LOCUS       DAJSKS010000001        92758 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJSKS010000001 DAJSKS010000000
VERSION     DAJSKS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 92758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 92758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.001"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            43..1101
                     /locus_tag="ON396_000001"
     CDS             43..1101
                     /locus_tag="ON396_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCR3112143.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            1105..2139
                     /locus_tag="ON396_000002"
     CDS             1105..2139
                     /locus_tag="ON396_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HCR3112144.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(2241..2957)
                     /locus_tag="ON396_000003"
     CDS             complement(2241..2957)
                     /locus_tag="ON396_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR3112145.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(2926..3711)
                     /locus_tag="ON396_000004"
     CDS             complement(2926..3711)
                     /locus_tag="ON396_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HCR3112146.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3702..4451)
                     /locus_tag="ON396_000005"
     CDS             complement(3702..4451)
                     /locus_tag="ON396_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR3112147.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4749..5273
                     /locus_tag="ON396_000006"
     CDS             4749..5273
                     /locus_tag="ON396_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR3112148.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5289..6788
                     /locus_tag="ON396_000007"
     CDS             5289..6788
                     /locus_tag="ON396_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR3112149.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6876..7478)
                     /locus_tag="ON396_000008"
     CDS             complement(6876..7478)
                     /locus_tag="ON396_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3112150.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7582..8538
                     /locus_tag="ON396_000009"
     CDS             7582..8538
                     /locus_tag="ON396_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCR3112151.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8843..9799)
                     /locus_tag="ON396_000010"
     CDS             complement(8843..9799)
                     /locus_tag="ON396_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3112152.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            9973..10533
                     /locus_tag="ON396_000011"
     CDS             9973..10533
                     /locus_tag="ON396_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112153.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10526..11350
                     /locus_tag="ON396_000012"
     CDS             10526..11350
                     /locus_tag="ON396_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCR3112154.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11360..11977
                     /locus_tag="ON396_000013"
     CDS             11360..11977
                     /locus_tag="ON396_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR3112155.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            11995..12678
                     /locus_tag="ON396_000014"
     CDS             11995..12678
                     /locus_tag="ON396_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR3112156.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13361..14644
                     /locus_tag="ON396_000015"
     CDS             13361..14644
                     /locus_tag="ON396_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3112157.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            14660..15547
                     /locus_tag="ON396_000016"
     CDS             14660..15547
                     /locus_tag="ON396_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3112158.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15564..16400
                     /locus_tag="ON396_000017"
     CDS             15564..16400
                     /locus_tag="ON396_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3112159.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16403..17026
                     /locus_tag="ON396_000018"
     CDS             16403..17026
                     /locus_tag="ON396_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112160.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            17023..18315
                     /locus_tag="ON396_000019"
     CDS             17023..18315
                     /locus_tag="ON396_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3112161.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18674..18771
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18887..19468
                     /locus_tag="ON396_000020"
     CDS             18887..19468
                     /locus_tag="ON396_000020"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR3112162.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19471..20778
                     /locus_tag="ON396_000021"
     CDS             19471..20778
                     /locus_tag="ON396_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCR3112163.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21194..21682
                     /gene="purE"
                     /locus_tag="ON396_000022"
     CDS             21194..21682
                     /gene="purE"
                     /locus_tag="ON396_000022"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR3112164.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21675..22799
                     /gene="purK"
                     /locus_tag="ON396_000023"
     CDS             21675..22799
                     /gene="purK"
                     /locus_tag="ON396_000023"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR3112165.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22812..24107
                     /gene="purB"
                     /locus_tag="ON396_000024"
     CDS             22812..24107
                     /gene="purB"
                     /locus_tag="ON396_000024"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR3112166.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24287..24664)
                     /locus_tag="ON396_000025"
     CDS             complement(24287..24664)
                     /locus_tag="ON396_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112167.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            24920..25648
                     /locus_tag="ON396_000026"
     CDS             24920..25648
                     /locus_tag="ON396_000026"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR3112168.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            25648..25902
                     /gene="purS"
                     /locus_tag="ON396_000027"
     CDS             25648..25902
                     /gene="purS"
                     /locus_tag="ON396_000027"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCR3112169.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            25904..26575
                     /gene="purQ"
                     /locus_tag="ON396_000028"
     CDS             25904..26575
                     /gene="purQ"
                     /locus_tag="ON396_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HCR3112170.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            26576..28798
                     /gene="purL"
                     /locus_tag="ON396_000029"
     CDS             26576..28798
                     /gene="purL"
                     /locus_tag="ON396_000029"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCR3112171.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            28783..30222
                     /locus_tag="ON396_000030"
     CDS             28783..30222
                     /locus_tag="ON396_000030"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR3112172.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            30245..31297
                     /locus_tag="ON396_000031"
     CDS             30245..31297
                     /locus_tag="ON396_000031"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR3112173.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            31294..31872
                     /gene="purN"
                     /locus_tag="ON396_000032"
     CDS             31294..31872
                     /gene="purN"
                     /locus_tag="ON396_000032"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR3112174.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            31869..33404
                     /gene="purH"
                     /locus_tag="ON396_000033"
     CDS             31869..33404
                     /gene="purH"
                     /locus_tag="ON396_000033"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR3112175.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            33420..34694
                     /gene="purD"
                     /locus_tag="ON396_000034"
     CDS             33420..34694
                     /gene="purD"
                     /locus_tag="ON396_000034"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR3112176.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            34816..35730
                     /locus_tag="ON396_000035"
     CDS             34816..35730
                     /locus_tag="ON396_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR3112177.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(35863..37428)
                     /locus_tag="ON396_000036"
     CDS             complement(35863..37428)
                     /locus_tag="ON396_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR3112178.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(37421..38872)
                     /locus_tag="ON396_000037"
     CDS             complement(37421..38872)
                     /locus_tag="ON396_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3112179.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(38886..39902)
                     /locus_tag="ON396_000038"
     CDS             complement(38886..39902)
                     /locus_tag="ON396_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3112180.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            40122..41210
                     /locus_tag="ON396_000039"
     CDS             40122..41210
                     /locus_tag="ON396_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HCR3112181.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            41264..42790
                     /locus_tag="ON396_000040"
     CDS             41264..42790
                     /locus_tag="ON396_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR3112182.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            42792..43997
                     /locus_tag="ON396_000041"
     CDS             42792..43997
                     /locus_tag="ON396_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3112183.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            44254..45231
                     /gene="guaC"
                     /locus_tag="ON396_000042"
     CDS             44254..45231
                     /gene="guaC"
                     /locus_tag="ON396_000042"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR3112184.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            45495..46919
                     /locus_tag="ON396_000043"
     CDS             45495..46919
                     /locus_tag="ON396_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR3112185.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            47380..48489
                     /gene="pdhA"
                     /locus_tag="ON396_000044"
     CDS             47380..48489
                     /gene="pdhA"
                     /locus_tag="ON396_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCR3112186.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            48492..49469
                     /locus_tag="ON396_000045"
     CDS             48492..49469
                     /locus_tag="ON396_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCR3112187.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            49494..51137
                     /locus_tag="ON396_000046"
     CDS             49494..51137
                     /locus_tag="ON396_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR3112188.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            51145..52551
                     /gene="lpdA"
                     /locus_tag="ON396_000047"
     CDS             51145..52551
                     /gene="lpdA"
                     /locus_tag="ON396_000047"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR3112189.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            52713..52988
                     /locus_tag="ON396_000048"
     CDS             52713..52988
                     /locus_tag="ON396_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCR3112190.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            52994..53761
                     /locus_tag="ON396_000049"
     CDS             52994..53761
                     /locus_tag="ON396_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HCR3112191.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            53929..55758
                     /gene="typA"
                     /locus_tag="ON396_000050"
     CDS             53929..55758
                     /gene="typA"
                     /locus_tag="ON396_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCR3112192.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            56019..57791
                     /gene="recQ"
                     /locus_tag="ON396_000051"
     CDS             56019..57791
                     /gene="recQ"
                     /locus_tag="ON396_000051"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCR3112193.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            57905..58207
                     /locus_tag="ON396_000052"
     CDS             57905..58207
                     /locus_tag="ON396_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCR3112194.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            58210..59373
                     /locus_tag="ON396_000053"
     CDS             58210..59373
                     /locus_tag="ON396_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR3112195.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            59399..62827
                     /locus_tag="ON396_000054"
     CDS             59399..62827
                     /locus_tag="ON396_000054"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCR3112196.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            62848..63972
                     /locus_tag="ON396_000055"
     CDS             62848..63972
                     /locus_tag="ON396_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112197.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            63962..64399
                     /locus_tag="ON396_000056"
     CDS             63962..64399
                     /locus_tag="ON396_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR3112198.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            64383..64721
                     /locus_tag="ON396_000057"
     CDS             64383..64721
                     /locus_tag="ON396_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCR3112199.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            64840..65400
                     /gene="rsmD"
                     /locus_tag="ON396_000058"
     CDS             64840..65400
                     /gene="rsmD"
                     /locus_tag="ON396_000058"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCR3112200.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            65397..65888
                     /gene="coaD"
                     /locus_tag="ON396_000059"
     CDS             65397..65888
                     /gene="coaD"
                     /locus_tag="ON396_000059"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR3112201.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            65878..66921
                     /locus_tag="ON396_000060"
     CDS             65878..66921
                     /locus_tag="ON396_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HCR3112202.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            67080..67808
                     /locus_tag="ON396_000061"
     CDS             67080..67808
                     /locus_tag="ON396_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR3112203.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            67924..68169
                     /locus_tag="ON396_000062"
     CDS             67924..68169
                     /locus_tag="ON396_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR3112204.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            68166..68384
                     /locus_tag="ON396_000063"
     CDS             68166..68384
                     /locus_tag="ON396_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR3112205.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            68520..69059
                     /locus_tag="ON396_000064"
     CDS             68520..69059
                     /locus_tag="ON396_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCR3112206.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            69052..71325
                     /locus_tag="ON396_000065"
     CDS             69052..71325
                     /locus_tag="ON396_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCR3112207.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            71363..72409
                     /gene="holA"
                     /locus_tag="ON396_000066"
     CDS             71363..72409
                     /gene="holA"
                     /locus_tag="ON396_000066"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR3112208.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(72498..72752)
                     /locus_tag="ON396_000067"
     CDS             complement(72498..72752)
                     /locus_tag="ON396_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR3112209.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(73152..73421)
                     /locus_tag="ON396_000068"
     CDS             complement(73152..73421)
                     /locus_tag="ON396_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112210.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(73891..74346)
                     /locus_tag="ON396_000069"
     CDS             complement(73891..74346)
                     /locus_tag="ON396_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR3112211.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(74484..76412)
                     /locus_tag="ON396_000070"
     CDS             complement(74484..76412)
                     /locus_tag="ON396_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCR3112212.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(76609..77442)
                     /locus_tag="ON396_000071"
     CDS             complement(76609..77442)
                     /locus_tag="ON396_000071"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR3112213.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(77634..78338)
                     /locus_tag="ON396_000072"
     CDS             complement(77634..78338)
                     /locus_tag="ON396_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR3112214.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(78361..78987)
                     /locus_tag="ON396_000073"
     CDS             complement(78361..78987)
                     /locus_tag="ON396_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3112215.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDVERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            79313..81689
                     /locus_tag="ON396_000074"
                     /pseudo
     CDS             79313..81689
                     /locus_tag="ON396_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
     gene            complement(81713..82204)
                     /locus_tag="ON396_000075"
     CDS             complement(81713..82204)
                     /locus_tag="ON396_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HCR3112216.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            82391..83311
                     /locus_tag="ON396_000076"
     CDS             82391..83311
                     /locus_tag="ON396_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3112217.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            83465..83809
                     /locus_tag="ON396_000077"
     CDS             83465..83809
                     /locus_tag="ON396_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCR3112218.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(83999..84499)
                     /locus_tag="ON396_000078"
     CDS             complement(83999..84499)
                     /locus_tag="ON396_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3112219.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            85027..85110
                     /locus_tag="ON396_000079"
     tRNA            85027..85110
                     /locus_tag="ON396_000079"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:85061..85063,aa:Leu,seq:cag)
     gene            complement(85434..86072)
                     /locus_tag="ON396_000080"
     CDS             complement(85434..86072)
                     /locus_tag="ON396_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3112220.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            86407..86841
                     /locus_tag="ON396_000081"
     CDS             86407..86841
                     /locus_tag="ON396_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR3112221.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            86864..87184
                     /locus_tag="ON396_000082"
     CDS             86864..87184
                     /locus_tag="ON396_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112222.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            87325..87516
                     /locus_tag="ON396_000083"
     CDS             87325..87516
                     /locus_tag="ON396_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112223.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(87587..87844)
                     /locus_tag="ON396_000084"
     CDS             complement(87587..87844)
                     /locus_tag="ON396_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HCR3112224.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            88002..88265
                     /locus_tag="ON396_000085"
     CDS             88002..88265
                     /locus_tag="ON396_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112225.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            88289..88477
                     /locus_tag="ON396_000086"
     CDS             88289..88477
                     /locus_tag="ON396_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112226.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(88560..88745)
                     /locus_tag="ON396_000087"
     CDS             complement(88560..88745)
                     /locus_tag="ON396_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112227.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            88960..89331
                     /locus_tag="ON396_000088"
     CDS             88960..89331
                     /locus_tag="ON396_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR3112228.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(89481..89553)
                     /locus_tag="ON396_000089"
     tRNA            complement(89481..89553)
                     /locus_tag="ON396_000089"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(89518..89520),aa:Gln,seq:ctg)
     gene            complement(89599..90027)
                     /locus_tag="ON396_000090"
     CDS             complement(89599..90027)
                     /locus_tag="ON396_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112229.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(90087..90845)
                     /locus_tag="ON396_000091"
     CDS             complement(90087..90845)
                     /locus_tag="ON396_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCR3112230.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            91140..91337
                     /locus_tag="ON396_000092"
     CDS             91140..91337
                     /locus_tag="ON396_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112231.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            91443..91925
                     /locus_tag="ON396_000093"
     CDS             91443..91925
                     /locus_tag="ON396_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112232.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(91983..92579)
                     /locus_tag="ON396_000094"
     CDS             complement(91983..92579)
                     /locus_tag="ON396_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR3112233.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT        30156 a        15935 c        19782 g        26885 t
ORIGIN      
        1 tttttttatt gtgaataaat gaacataatt aggaggagaa agatgaactt atttgacatt
       61 tttcgatcga ctttgacaga tatgtcgatt gtcagtgcaa tcacttcaac agtttttatt
      121 atattattag ggtttgtttg tagaaaaaag gggatttttt ctgaatctgt tggaaaaatt
      181 ctatcgaaag tcgtcttaac agtggctttg ccagcactgg catttcgagc tttcatgcaa
      241 gatatcaata cagaattact gatgcaagga atcaatgtct tgatttgggg attagttatt
      301 tatattattc ttattctagt ttcaaaacct tttttcttaa gctataaagg ggataagcaa
      361 acaacgttgc gtgtcctgac gatctttggt tctacgacct ttttcggaac gcctatcgtc
      421 agtgcgattt atggtcctgt cggcgtgatg tattcctcaa ttttcaatat tggctaccga
      481 atctttttgt attcttatgg atacataaaa atgagtggct tgaagatgga agctaaaaat
      541 atcaaagcga tgttcgtcaa tccaatcatt atcgctactt ttgccggcct tttcatttgg
      601 ctgttccaag ggtatctgcc acaagtgtca gttacaggtg cagaaggaga gataagccaa
      661 gtagcgtttt tgcgaattga ccagactgca ccgtggctat ttcagccgat ggattattta
      721 gctggtttaa gttcaccatt ggcgtggcta gcaatcggcg caacattagg tgaagtaagc
      781 ttcaaaaaag ctgcatctga aaaaacttca tggtattaca gtctagtcaa agtggtattt
      841 gtacctgcat tgaatatcgt gattctagca gttttgacgg caacgaatat tctaccagtc
      901 aattttgaag cactggcaac aatcgttatc atgatggcta caccagctgc aacagtggca
      961 gcagcctatg ctatcagttt tgatagggag gcacttttag cctccaatgc atccttactg
     1021 tcaactattt ttgctgtggt actgacacct gtatggatcg ttgtattaca aatgattcat
     1081 ggcataggtt tattttcata agaaatggag gaagaaagta tgagaatcgc atgttacggt
     1141 gttcgtccga acgagataga tttttttgaa aaattgaata tttaccatta tgatctcagt
     1201 ctttatgaag aactgttgac acatgacaat atcgtgtcag caaaggacca tgatgctgta
     1261 ttgttgaggg gaaattgtat agcagatgag accaatcttg caaaaatgca ggaatatggg
     1321 attcgttatg tttttacacg gactgtggga gtcaatcata tcgatctaca agcggcagct
     1381 gattttggta tgacagtagc ccgtgtacca tcgtattcgc caaatgcgat agcagagtta
     1441 tctttgactt ttgccatgat gcttttgaga aatactgcgt atacgacgat ccgaacatct
     1501 ttcaaagatt ttcgagtgga tgagcaaatg tttagtcgtg aaatcagaaa ttgcacagta
     1561 gggatcatag gaacaggcag gatagggttg acggaagctg ttttgtttaa gggactagga
     1621 gccaaagtga tcggctacga tattcatcag acagaagctg ctaaaaaagt attgacgttc
     1681 cactcgcttg aagagttatt agccaaaagc gatattgtca gtattcatgt acctcatatc
     1741 ccaggtgaaa acgatcaaat gatcaatgaa ggatttttag ctcaaatgaa aaaaggtagc
     1801 atcttgatta atacagcccg tggagagctt caagataatg aagctatttt aagagctttg
     1861 aaaagcaatc atttagctgg ttttgcaacc gatgtttttg caaatgaaac agaggttttt
     1921 ttccgatcct tcaagccatg ggagacgatt ccagaccctg ctatccagca attagtagaa
     1981 atgtacccta gagttcttgt aaccccgcat gtaggttcaa atacagatga ggcattatca
     2041 aatatgattt cgacaagttt cgagaacttt cgtgaaataa tagaaactgg aaaaacgaaa
     2101 aatgaggtat ctcttcctaa agcccgtcaa ttaagataag aaaacatgga tattcgtcaa
     2161 taaaatgttt aaaaatagtc caagtaaaca gttgttactt ctgtatactt ggactatttt
     2221 gtatgggata attcgatgac tcaacagtaa tctgtgagct ttttccgccc attctttgtc
     2281 aattcataac aggttagcgg ccggccgatt tccaaatatt tgattctttc gttcaatata
     2341 tcttgttcta ctaaaaaatt caaatatttc ttgacggata tacgtgacag tgagcttttt
     2401 tgagccagtt cttttgttga aaaaatagta tcttctttta gaataaatgc acttattttt
     2461 tttaaagtca gttttgacag acctttaggc aaatcgatga atgatggtat ttcgtgaatg
     2521 ggagtggtgt ggttgaatac ttgatcgatc aatttctgat cggctttttc agtcgtttgg
     2581 attgtctgag agaaagcaag atacttttcg atcgccagct gaaaacgttg caaagtgaac
     2641 ggtttgatca aataatctaa ggcaccgtag cggatcacat tctttatggt ttcgatatca
     2701 tctgcagcag tgatcaaaat cactgggata tcatttcctt gctgtctaag ccaagataaa
     2761 aaatggatac cactttcgcc aggcatgtat acatcaagca gcagcagatc tgcgtttttt
     2821 tgtctgaaat atttttttcc ttcttctaca tttctgaaac tttcgattac gttcatatta
     2881 gggatgcttt ccacaaaagt acgattcaat tctgcaacca ttggatcatc ttcgattatc
     2941 aggacatttg tcatcatttt gatcttcctt ttttggtaat tctagataga cgattgtacc
     3001 gccatttgtt tgtttggaaa attctaaaaa tccaccgtgg cgtttgacga ttgaatggat
     3061 gagattcaat ccaaacccac gattgattcc cttcgtcgaa aaacctagcg taaatatttt
     3121 ttctgagatt tcttctgata tcccgacacc gtcatcgcag acggtcacga tgaatacttc
     3181 agcttctgtg tcatagttta aatgaatccg gacctgtccg tttctttcat gtttttgtag
     3241 cacggcttct tttgcattat caagtaagtt ccctaacact tgcaaaagtg aatggatgga
     3301 gtcatccatc tccattttag ggataaaaga atcgctttct aacagcattt caacagattg
     3361 ttctttggct tcgtttattt tgcctaataa aaaaccggca attgcaggta ctttgatcag
     3421 acttgagact tggccaatct cttttttata attttgattt agctgctgga taaaatgggt
     3481 gacttcttca tacttcttca aatcaatcaa accagaaatg acatgaagtt tattcatgaa
     3541 ttcatgggtt tgagctctca tggagtttat atattgtttc gtccccccaa gctcctccat
     3601 taatttcatt acttctgatc gatttcgtaa agttgcgact gctcctaaaa aacggtcatt
     3661 gctaaaaaga ggtgtaacgc tagaaatagt ttctatccca tttaaataca tggattgagt
     3721 ccgaacggtc gtttgatgct caatcgtctc tttaaacaaa acgtcatata gattatcact
     3781 gatttttttc ctacttcgaa atccaatagc tgctctgttt ggtagaaaat gttttttgca
     3841 ggtgtattga tcaagaggat agttttatca gaagagatgg ctaagattcc ttcatctact
     3901 tcattttcga taatcgtttt ttcacttaac tgttctgcta tttcttgtgg ttcaagtcca
     3961 agcagtactt ttttcatttt ttgggcaaga aaaacggcgc ctatgatgcc tacgctaagt
     4021 ccaataatca atgaaaaaac aatcattttt tgtccatctt tgacttgatt gtcaatcgtg
     4081 ttgtttgtta agccaacaca aactgcacca ataatctctt gatcataata aatagggacg
     4141 aaatagcgga agccgaaccc taacattcct tctttttcag agaaaaattc ttgtcctcca
     4201 atcacttttt tcacatcttc atgacttgag aaaggaagac cgatgtaact tgggtctgcg
     4261 tgagaatatc gaatgccttc agggtccatg acaacgataa aatctaaatt cgatgcaatc
     4321 atgatttcat ttgttattcc ttgaatcgca tgtgtcgggt caccagtctg cagctttttt
     4381 tggatgagtt tatcattggc aacgatttta gcaatgctag agattttatc tgacacacta
     4441 gatcggttca tcgaaagaat aaaattcctg atcaatatgg cagaaacaat caaagaaacg
     4501 aatgtggaga aacaaacgac aaggatgatc gtgttctgga gctttaatgg ttttttctta
     4561 aaaaaaggaa acataggcta cctcgaaaga atttattgtg aaaattttaa catgaaaaca
     4621 aaaatttaac aaattgtcaa cgataaacac aaccttccta taaaaaacac cactgagtcg
     4681 gtaaaagaag ctatacagaa ttttcaaaca ttggtatgat agacgtagaa atggaaggag
     4741 gcgtgtatat gataggggga atcgcacttg agacgattta ttggtataca ttgctgattt
     4801 gtgcaggatt ggcagtccta ttgattattt ttggagatgt attcgatttt gatggcccgc
     4861 ttgatccgat gcttattatc ccttggttaa ctttcacaag cttatttggt tttctaggtg
     4921 agcgtttaac agatcaatca tctttatttt tatttattgc tagtggaggc atatcgatag
     4981 tgttggtatt tttgcttaat ttctatatat tgatgccgat gaaacatgcc gaatcaacga
     5041 tgtcgtcttc tgaaaaaact ctggaaggac aagtagctgt tgtgatcaca ccgatccctg
     5101 tacgaggtat gggagaaatt cagttgaaaa gtgttaccgg ttccattagt cgtccagctt
     5161 gtttttacag cccccaagaa cagacagctg agcggggaac gaaagtattg atcattgaga
     5221 tcaaggaaag aatctgttat gtcatgcctt atgaaagcat gataaaagct taaaggagcg
     5281 tgtgttaaat ggaaatacca gttgttttta tcgcgatcgc aatcgcagct ttgattttac
     5341 tgatgttgct gatcgtcttt gtagcgaagt accagacagc aaaaccggat gaagcgttga
     5401 tcatcagtgg aagttattta ggaagcaaaa atgtgcacat tgatgaaagc aataacaaaa
     5461 tcaaaattgt gcgtggaggt ggcgcattcg tcttgccagt ttttcaacgt tcgaatcgga
     5521 tcagtctatt atcgagtaaa ttggacgtct ccacccctga agtgtatacg gaacaaggcg
     5581 taccggtgat gtgtgatggt acttcgatca tcaaaatcgg ttcatctgta gaagagattg
     5641 ctacagcagc agaacagttt ttaggcaaga caagagaaga actggaaaat gaagcaagag
     5701 aagtattaga aggacacttg cgctcgattc ttggttctat gactgtagaa gaaatctatc
     5761 aaaatcgcga taagttcagt caaagcgtac aagaagtcgc ttctgtagac cttgcaaaaa
     5821 tggggttgat catcgtttct ttcactatca aagaggtcag agacaaaaac ggttatcttg
     5881 actctttagg gaaaccaaga atcgcccaag tcaaaagaga tgcagatata gcagaagcgg
     5941 aagctctaaa agaaacgcgt atcaaaaaag ctcaagcaga aaaagaatca caagcagctg
     6001 aattacagcg acaaacagag atcgcagaat cattaaaaga aaaagaactg aaattagcaa
     6061 cctacaaaca agaacaagat gttgctaaag caaaagcaga ccaagcctac aatttagaaa
     6121 gtgcccgagc acaacaacaa gtaatcgaac aggaaatgca gattaaaatc gtagaacgtc
     6181 aaaaacaaat cgaactggaa gaaaaagaaa tcacacgtcg tgaaaagcaa tatgattcgg
     6241 aagtgaagaa aaaagcagat gccgatcggt acgctaaaga acaagaagca caagctcaaa
     6301 aagtcaaaga agtgactgaa gccgaagcag aacgattccg tgtggaagca ctggcagaag
     6361 cggaagccaa taagacacgt ttagctggac aagcagaagc cgaagcagca ctagcaaaag
     6421 gaaaagcaga agccgaagca aaacaaaaga tcgccaatgc cttcaaggaa tatggcgaag
     6481 cagctgtctt gagtatggtt atcgatatgc taccgcaatt gatgcgcgaa gcagcacagc
     6541 cgctaggaaa tatcgaaaaa atctctgttg tcgatacagg aagcagttca ggagaaacca
     6601 gtggagcgaa ccgagtgaca aattatgcca caaatttgct ttcttctact caagaaacac
     6661 taaaagcgac aacaggactt gacttgaagg aaatcgtaga gaatttgtcg acacgtggga
     6721 ctactagcca agtaaatatg tatgaagcgc caaaagaggc agaacaaaac tcgatgaagg
     6781 aagaataaag tagtacactt caccgttcaa tcaaaaatgg aggaagactt tttaaagttc
     6841 ttctctccat ttttttattt tattgcaaaa gaaaattagt cctcgctatt tttcttttgt
     6901 ttttggatct tagcttctat ctcttgtaaa tttgttctta gcaatcgttc tttcttttgg
     6961 agttcgacca aatcttctga tagttcttcc ttcttttctg tttcaggagg acgaagaaag
     7021 aaattgatac cgattgcaat gaatgtccct ataaaagtat ctagtacacg ttggatagca
     7081 tacaaagcgg attcgccttg agggatgctc aacgcaatca gcaacaacgt agctattgcg
     7141 gaaatgatac cggaattatt gttaatacca tctgacacga cgatcacaat aataactaga
     7201 ataggaagca agatcaattc taccaaaaaa tcattgtgaa aatagctttt gacaaaaaaa
     7261 taaatgatag ctaatgtacc acctaatgta ttgccgatga tcctagactt tccaaaagaa
     7321 acggttgtag tcaagtcctg acgcaaagaa aaaacagcag ataatgcagc aatcatcggg
     7381 attcctcgtt gagtcaaatg aaaaaacaag atacataaaa aaacagctaa ggcacttttg
     7441 atcgtacgca tacctagtcg aaaacggcca aattgcatag agattctcct ttttggtctt
     7501 tttactatca tacctaaact tttgtctgat agctagtttt tcattattca agtataatta
     7561 aaataaaaaa gggggaagta aatgcgtata ggaatgcgaa cagtcaaaac agtgatcagt
     7621 gtcttctttt cgatggtcac agcgggtagc ttatcgctgt tgtattggcc agctgcaggg
     7681 atcattgcat tattaagtgt aggaaatacc aagcgctcta cattgcgtac gggcatgaat
     7741 cgaatcgcag cctttatatt agctacggca acagcactgt tcagtttttt tgctgttggg
     7801 tatactattt ttggcttcag tcttttttta ttcctgttca tcccgcttgc ctctcgcttc
     7861 aaattaaccg aagggatagt agtaaattcg gttttggtca ctcattattt agtggaggaa
     7921 aacatgtctt ggcagctgat cggcaatgaa gcgggattaa tggcgattgg attagtattt
     7981 gctttgcttg caaatgttta catgccagat cgtacgaagc agctgaagga aaaccaaata
     8041 cagatcgaaa aagaatttcg aaatattttg agacaaatgg cagaatttct tttatcagaa
     8101 aataaagaag atgtgcagat caaatgcgaa catttactga cattcattcg cgaaagccaa
     8161 gaagatgcca gagaacatca agaaaactac tggctcagac agccgctata ctacgaaact
     8221 tacttctcta tgagaagagc acaggccaat gtgattaaag acatgctgga aaatctagag
     8281 agaatccagc aaccagctta ttatggaaaa catatctatg gtttattgat ttatactgca
     8341 gaaacatttt cagagtcaaa tgatggcaga caaattttaa cacgtattga agaagtttat
     8401 gtattgtatc ggcaaatgcc tctgccgact agtcgccctg aattcgagga tcgagcagaa
     8461 ttgttccagt ttttgcagtc ttttaaaagt tttattgaaa tcaaggcaga attctcccaa
     8521 caaaaaatcc aaaaatagag gttgtgaatt cagctaatat tcaggtcaaa aagctgattt
     8581 cacaacctct tctttaaacg gtgttctcct gtcaactcct cggtcacaag tcacaatatt
     8641 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcggagc tgaacggtag
     8701 gctcacaacc tctttcttaa acggtgttca aaagctttcc tctgcggtaa taagtccagc
     8761 caaacgaaaa ataggaggag ctatttttgt ttggctgttc ttattacttg atgcagaaca
     8821 gctttttcac aaccttttaa aattacatat ttttccatag aacttgcatt tgatccaaga
     8881 tttcgatgac atcttttgtc aagaagagag agcgattggg caagtcacct tcaatcatgt
     8941 gtaccatatt ctttatttcg taattcattg cttcagatgt attcccgctt tcgatgaatt
     9001 cctttgtacc atctgtaaat aagatctccg cacgatcagc acgcgggtaa tcagcaatcg
     9061 tcacataacc gttctcaaag gcaacgatac cgacttttgg catttttgcc tgaaaagtca
     9121 agctgacagc agcaagttca ttttctttgt tacgcaagat agtgacggat tgttcgtcca
     9181 cgcctgtttc aaaaggaacc attgtagagg caactacttc aggttgagag ctcaaaaaga
     9241 aacgagcgaa agatacagca tacgtaccga tatccaacag agctcctcca gctaattctg
     9301 gattgaagaa ccggttttta gggtctggtt ctttgtaact gccgaaaggt gcttggatca
     9361 tttttaaagc ccccagtttt cctgtatcca ttattgagcg taactgctga taaagtggca
     9421 tgttgaagat tgtcatcgct tctgctaaga tcaaattctt ttcttctgct aagcgcatag
     9481 cgtcagccag ttctttttta ttcatcgtga tggctttttc acatagaacg tgtttgcctg
     9541 cttccaatgc tgccaagata tggtcgatat gctgtctatt tggtacagca atgtacacga
     9601 tgtcgatttc agcatcgtct aacatttctt gatagcttcc gtaagctttt ggaatattgt
     9661 aacggtgagc gaagttttcc gctttttctg aagttctgga agcaactgct gttagttcgc
     9721 tcgtctcttg attgaagctt tcggcaaact catgtgcgat ggtaccaaga ccaacgattc
     9781 cccattgata ttttttcatc tgaaaacgct ccttcatagt tggattgagt ttcttgtctg
     9841 tctattatta taacaaaaat cacgaagaat aaagaggaga ggacttgaaa cattaaaaaa
     9901 aatgaaagca gcgatttaag aggtgctttt tgatcaaaac ttaagtagaa tagttaaaag
     9961 gaggggataa acgtgcatca agtatatatt gatcttatta tgagtgtatt attagaacag
    10021 tttgaaactg aaaaaaattt tttagatgaa tacttgctga tcagtccaga acaatggcaa
    10081 aaatggaaag atggcgagaa aaatctgcca tctgaagcga tgcagaaagt aaaaaatctt
    10141 ttcactgatt atgaatggat gctaacgcaa aagattttgc gtcaaacgat tatttttccc
    10201 gaaaaacgaa acgttgccgt tgcagagtac aagcaaatca aaacacgcat cgcgcaaaaa
    10261 tggcttagca gcaaagcagc aaaagtcgag ctgatccctt tgaaaaataa tcagcaggct
    10321 ggtagatacc ttgacttgaa agtaagtatt caatatgatg aatggggcta tgatgatatt
    10381 ttgaattttc gtctgccagc agatgttcaa aaacagatcg aaggagaaaa aatcgaactt
    10441 ctcgattggg tgaatgaaaa tctggaagaa acttacgttc atgaggcgaa tggggaacgt
    10501 gaagaacaac gaaaggattt ttcaagtgaa taaattttta ggatttggag cagttgtgat
    10561 tatgatcgca gcaatgggct atgttatttt tagcgatgat gattcgcatg ttcaagccgg
    10621 caatcaagca gtcgaacgaa caagcaccaa aacaactgct agtaaagaaa agaacagtca
    10681 aacaaccagc agcaaggtaa aggacaaaga aacaaaagat cgtacgtcca aacaattgcc
    10741 agatgttaaa acagacgatt ggaagctagt tttggttggt ccagatcaca aattagaaaa
    10801 agaaattgat gagtctactc aattggcatc attaagtaac ggatacttga tcgacaaacg
    10861 cattgctcca gaatatgaag catttgccaa ggctgctgaa gcagctggat ttccattagt
    10921 gatggtctca gcttaccggt ctgtatcatc ccaacaacaa gttttcgaac aaaatgtcca
    10981 agatgtgatg agtcgtcaag gtgtatcaga agaggaggcg acaaagatta ctaaagaaac
    11041 gattacggag ccaggataca gcgaacacca cacaggtctg gcagtcgatg tcgtcgatca
    11101 aaactggtac aacagttatc cttctactgt attggatgca agttacggag atcagcctgg
    11161 agctaaatgg atcgcagata atgctgcgca gtatggattt atcgttcgtt atccaaaagg
    11221 acacgaagat ataactaaga tcacatatga accttggcac ttacgttatg tcggtaaaga
    11281 aaacgctgag tatatcacca aacaccaatt gacgctagaa gaatatctga atgaattaaa
    11341 tgaaaagtag gaattgatca tggcgaaaaa aaagtggaaa aagcaaaagc gcaagaatat
    11401 ccgttggcca gcagtggttg ccggtgtagc agtgatactc ttagcctttg ttttttcctt
    11461 gaagaatctg tcttctcctt ttactgataa tcagcaagaa aatgaagcgc aatcccatca
    11521 gcaatttatt gaacggcttg ttccgcatgc ccaagaacta caagatggtt atggagtgct
    11581 tcctagtatc attttaggac aagctatatt agaatcaaat tggggaaaaa gtaccttggc
    11641 tagcaaatac aataatcttt tcgggatcaa ggcttatggg gatcagaaga aagtaagcct
    11701 ggaaacaaag gaatttgtta acgaagaatg gataactatc caaggagact tcaaagtgta
    11761 tgattcatgg gaacagtcca tggatgacca tacacaactg ttcgtccaag gagtagattg
    11821 gaatcctgcg ctgtatgaaa aagtgattac ggccaccaac tatcaggaag ccgcacaagc
    11881 attgcaagat gcaggttatg cgacagaccc cggttatgcg caaaaaatca tccaggtcat
    11941 cgagacgtat caattgaatc agtatgaccg gagatagcaa aggagaagtg gctgatggca
    12001 gaacaattta cgccagaaat catgacggaa ttgagaaaag atatcgtaag agtaccggaa
    12061 atcattttag aagcaagcgg gatcaagata tttggaaaat tgatccgttc gatcatcttt
    12121 actacggata ttgcgatcat acgtaataca aatgcgaatg cagtaattgc tgtgtatcct
    12181 tttacacctc atccagcgat tactaaaagc attattgagg cagctgacat tccagttttc
    12241 tccggtgtag gtggtggttt gacacaaggt gctcgttctt cctacatgag catgttcgca
    12301 gaagcgcaag gttcgttagg ggtcgttttg aacggtccta cgcctttaga gacagtaaaa
    12361 gaagtttgtc gagtagttga tattccagtt atcagtacgg taacgtcgaa atatacagaa
    12421 atagatgaaa agttagcagc tggtatttcg attatcaata tcagtgcagg taaagaaact
    12481 gcagaagcag tacgccattt tcgtaaaaaa tatcctgaac ttcctatcat tgcgacaggt
    12541 ggtccaacgg aagaaagtat tgctgagacc attcaagcag gagcaaatgc catcacatat
    12601 acgccaccaa gtagcggaga gctttttagt cgtaaaatgg aaaaatatcg ggaaactgag
    12661 aaaaagaatc atgaataaca ggtgagaatc aaaagacgag gaagagcaga tagtggctct
    12721 tcctcgtctt ttgtactcca gtactatttg ttcagaattc aaaaaaaaga gaaatttttt
    12781 tgttttttta cctgtaatgt acataagaag agttaaaacc ttttgttatt attagcattc
    12841 taacaaaaaa atgtcttgat ttattgaagc gctttctata gaatgaaaaa cgaataaaaa
    12901 ctaaaaaagg cacccgacaa tttagtagca gctaaattgt cggggcttga aaagtcagcg
    12961 ggaactgacc aatcaagttg ccatagtcaa tgcttgtgca tcgacgtgta tattgtacct
    13021 aatttcatac agaattacta gttatttttc tgttttttag ggaagagcgc acacgaagaa
    13081 gcatgcttat ttagctatgt caaggatatt gggcagttgt ctgatatcct tttttctttg
    13141 cgtaattatc ctcccatcat tgaagcaaga agtgttttta gcgatgaaag aagaatcagc
    13201 actatctaat ttttcttgaa agtattgagt tgttcatcct aggcagaacg ataaggcaag
    13261 tcttggtttt tattcccctt gaaaaagatc gtttaataaa tatgttctgt ataaaaatac
    13321 agtatattga aaaataacaa tggaagtaga ggtgcagaga atgaagaaaa gaatgagaat
    13381 catcttagct ttaagtgtta tgttattctt cttgacggct tgtgggcaag aagatgacaa
    13441 aaaaatcacg ctgaacatgg cttggtgggg ttcgcaagtc cgacacgatg caacagtaaa
    13501 agtaatcgag ctttatgaag aaaaaaatcc ccatgtgacg atcgattatg aattttatga
    13561 ttatgaagga tattacacaa aattaggtac actagcagca tcagatgata tctgggacat
    13621 tttccaactg aacaatcaat ttcctcaata catcaatcag attgaaccat tagatgaata
    13681 tgtggagcaa gggataattg atatttctaa tgcagatgaa tcctatttag caacgacgac
    13741 ttacgacggg caattagttg gactgtctaa cggggtgaat tcgtttgcga ttgcttacaa
    13801 taaaaatata tttgaacaat taaatatccc tgaaccagct tcaaattgga catgggatga
    13861 atttgaacaa atcagctatc aaatcactga agaattagga atctatgcta tttcaagatt
    13921 cgaagatttt atagctggct gtattattca aataccacaa gttgagaaag gattgaattt
    13981 ttataatcgg gaagatctat ctaacagctt gggatttgaa aatccagact atctaacaga
    14041 ttttttccag ctgcgtaaaa atctggtaga caaaggggcc catccagaac caggtggtgg
    14101 agctgcaagt acgacggatc tgtcttttca ggcggttagg gatagagaag cggcaatggt
    14161 gtttctatca agtaaccagt tgacagaaat attgtcaggg gctcctgaag atatggagat
    14221 tcagttgatt aatccacctc gcagaaaagc agatggagaa tctggtatcc ctttacgttc
    14281 ttcgcaaatg ctgagtatgg caaaaagctc agcccataaa gaagaagctg ctaagtttat
    14341 tgatttcttt caaaacagtg aagaagccaa tgaaatttta aaaggagaac gtggtgtacc
    14401 aatcatgtcg aatattcgag aacagctcgc tcgtgcagat gatgcaactt tgcaagcgac
    14461 atttgattat ttagaaatga tttccgatat ggacaacggc gaagtgaatg tctttgatag
    14521 tgctttgaat ccggaaattc aagaccaata caatctttta gttgaaaaag tcatttatga
    14581 agaagtaaca gcagaagaag cagcagagag tctttatgag tttgcgtcaa acattctagg
    14641 atagcagcag gaggaaggca tgaaaaaaga aaaactaaaa cggttcttgg atcaagacaa
    14701 tgtggtcggt tacgtatttg cttctccatt catcctagga tttctattga taacagtgat
    14761 accgatgatc ttgtcacttt attattcgtt caccgattat cgtttgggaa atccagtctc
    14821 atggatcggc ttggataatt atataaagat gctgagtgat gcccgttttt tgaactccat
    14881 ccgagtcact tttctctatg tatttgcatc tgtacctgtg aaactgatct ttggtctatt
    14941 gattgcttat cttttaacga ggaatatcaa aggaacgact ttctttcgtt ctttgtatta
    15001 tgtgcctagt ttgatcggtg gcagtatttc tatcgctcta gtttggcggg agttgttttc
    15061 taaaaacggg ttagtgaata ttttcctgga acgattaggc gcacctgcgc tttcctggtt
    15121 tagcgatcaa aaactggtca tgatccctct tgttttgatg tctgcttggc aattcggttc
    15181 ggcaatgatc atctttgctg caggtatcaa agagatccca ggctcgtatt atgaagcggc
    15241 agagatcgat ggagcgaaca aatggcagac gttttacaag atcacgttac ctcttctttc
    15301 gccgatcatt ttgtataact tggttatgca gacaatcgcg gcgtttatga cttttacgca
    15361 ggcatttatc attacccaag gtggtccgaa tgatgccacg aacttttatg ctctttatgt
    15421 gtataaccaa gctttttctt ttaattctat ggggtatgct agtgctattt cgtggatcat
    15481 gctgttgatt atgggagctg tcacctttct gattttcaaa tcatcaaaaa aatgggtcgt
    15541 tgtgtgaatg agaggaggaa gaaatgaata atacagtaac gaaatggttt caatacggtt
    15601 ttattttgct attaggtcta gtcatgattt atccggtctt atggatgatc gcaggttctt
    15661 ttaaaacgga tcaagagatt atgagtggtt cgctgaattt gattccagag acttttcaat
    15721 gggaaaatta cgcaaaagga tgggcggggt ttgctggaat ttcatttttt actttcttta
    15781 ggaattcgtt tgtcatctct gcagtttcga cgatagggac agttctgagc tcgacttgta
    15841 ttgcttacgc attatcgaga atcgatttca aaggaaaaag attctggttc gtcgtcatgt
    15901 tagtaacgat gatgattcct gcacaagtga ttttgattcc gcagtttatt atttacaatc
    15961 ggctgaattt ggttgggacg tatgtgccgc tgattttacc acattttttt ggacaagcat
    16021 ttttcattta ccagatcatg caattcatgg tcagcatccc caaagagtta gatgaatcag
    16081 cgattattga cggatgcagc aagtatagtg tctttaccaa aattattttt cctttgctga
    16141 aaccttcgat catcacgacg atcatcatcc agttttattg gaaatgggac gacttcatgg
    16201 gaccattgat ttatttaaac aagccgagat cttacacggt atcgattgcc atcaaattat
    16261 ttgcagacgc tggatctagt acgacttacg cgagtatgtt tgcgatgtct acgttgtcgc
    16321 tgctaccggt ttttctgatt ttcttattct tcaataaata tcttgtccag gggatcagta
    16381 caagcggtct gaagggctga atatgaaaaa gccaatagaa gagatgtttc tttacaagct
    16441 caactactat gtctgtcgga tttttttaac cagtcttatc agtaatgctc ttttcgcttt
    16501 agttctagga tgtttgttca ctttaggatt agtgctgtct gctcgtgttg tctatctgat
    16561 ttattttttg atgatgtatc cttcttatta tctatctgtc cattacttgg cagaaaaaaa
    16621 ggtatgttgg aggaaaggaa aaatcaaacg gatgtgtttc atcattacgt tgtttcagct
    16681 gtttgtcatt atttttgcag ttgattggta tttattagtg ggaaagattg gagcttggta
    16741 tctgattcct tttctattgt gtctgttgct ttttgtatcc catagttttt ttagtacgct
    16801 aattgatatc tgcaaagaag ataaagcaga tagtcttgtt tatttgatca aaaggaatat
    16861 ctggctgttg tcaaaaaacg tcaaggggtt tctgtggtat ttatgccttt tgtatggact
    16921 gttctggtgt atacagcatg attttttact cactgcatta ctgtatccag ggctgccatt
    16981 tgtcacagta ttttgcttaa aagaaaagga gaatgacttt aaatgaagaa aaaatatgta
    17041 caagtcggac ttggtgggag agcgcgtttt ttttatgagg caattgcctc tacgtatcat
    17101 caaacttgtg aactagtcgg tttttgtgat gtatctcaaa caaggatgga ttatgcaaat
    17161 cggatacttg tgaacaagta tggacagaaa aaagtaaaga catgtatgcc atcacaattt
    17221 gagcaactac tggatgacgt acggccggct gaagtcatta ttacgtcagt agatcgaacg
    17281 catcatgatt atatcattcg tgcaatgaaa aaaggatatg atgttatttg cgaaaaacct
    17341 ctgactatcg atgaggaaaa agctcaagaa ataataacgg tccaaaaaca gacgggaaga
    17401 agcctgcgag tcacttttaa ttatcgttat gcaccgtatc acacgaagat aaaggaactg
    17461 atagaaagtg atgtgattgg tgatgtgttt tctatccact tcgaatggct gttaaatacg
    17521 caacacgggg cagattattt cagaagatgg catcgaaata agcacaatag cggcgggctt
    17581 cttgttcata aatctaccca tcattttgat ctagtgaatt tttggttgaa cagccgtccc
    17641 caaaaggtat ttgcattcgg tggattaaac ttttatggga tgcataatgc ggagaaacgc
    17701 ggagtagaaa tattttacag tcgatgctat ggaagcgaac tagctaaagc tgatccattt
    17761 gctatagacc tgtcagccaa tgaggaattg cgagaaatgt atttagaagc agaaaaagac
    17821 agtggatata ttcgtgaccg cagtgtattt tccgatgata tctctattga agatacacta
    17881 ggcgtaacgg tcaaatatga aactggtgcg attttgacgt attcgctaaa cgcgtatatg
    17941 ccgtgggaag gattcaatgt ttctttcaat ggcagcaaag gacggatcga aatgaaagtg
    18001 atcgaaaagt cttacatcaa tgcaggtgga gataaaaagg atgaaggagc agccgttctt
    18061 cagtccatcc atgtctatcc gatgttcggt gagccatatg aggtaatcgt cgaacaagaa
    18121 gcgggcggac atggcggagg agatccgcgt ttattagatg atctatttgg tgaagaaaaa
    18181 gaagaagatc cgtggaatcg ggcagcaact cacgtagatg gtattttgtc gattttgaca
    18241 ggaatcgcag caaatcattc tattgcttca ggaaaagcag tagtgattga cgagcttgtc
    18301 agcttaaaag aataagaata gatttacgga aattacttcc cgtagtttat aaaatcttgc
    18361 cttattgccg ataggctgtc ttttgaacac tatttattcg gaaaaagggg ctatgacatg
    18421 ctttttgtca tgccttcttt ttatgtgggc tgctttttta gataggagaa aataaaaata
    18481 actatttaaa cccgaacaaa aaggaataat gcattaaaaa tcattagatt ttacaaaaaa
    18541 ataagtaaac cacgttgtca gtttcagaaa gtagtgttag actgttttca atatttgaaa
    18601 caacattttt tattcaaata tggcaaactg ccttcacgtg caaaacagtt gcaagaaaat
    18661 gaatttacac tattattagt tttacatacc tacttatata tcgtcataat atggatgaca
    18721 gtttctagcc agtaccgtaa atgctggact ataagtaaaa gattggctat ttcacccttc
    18781 ttttcttatg ataaagtgga gggtgttttt ctttaacaga aacatagtag gtttttgcac
    18841 aaaagcggtt atttcggtat taataaatga taaaggagca aataaagtga aagaattagt
    18901 tgaacgtatt caaaaagacg gacgtatatt aggcgagggc gtattaaaag tcgacagctt
    18961 cgtcactcac caagtagacc ctgtattgat ggaacaaatg ggaaaacggt ttgcagaagt
    19021 atttaaagaa caaggaatca cgaaagttgt gaccattgaa gcttctggga tcgcaccagc
    19081 tttatttgct gcaagagaat tagatgttcc aatgatcttc gctagaaaag caaaaagttt
    19141 gacgatggat gaagaattat tgacagcttc tgtttattct tttacgaaac aagtgacaag
    19201 tacgatttct atttctcgta aatttttatc ttcagaagac aaagtattga ttatcgacga
    19261 tttcttggca aacggacaag cggcaaaagg attgatcgaa ttatgccagc aagctggagc
    19321 gcaagtagaa ggaatcggga tcgtcatcga aaagtctttc caagatggac gtcagttact
    19381 tgaagaaatg ggattgagag tcgtttctct tgcgcgtatt gcttccttga cgaatggaca
    19441 agttgagttt cttgaggagg acgcataatc gtggaaaaag aaacacaaaa tggaaaagct
    19501 gcggttttag ggctgcagca tctgctagcg atgtatgcag gagcagtagc ggtgcctctt
    19561 ttgatcggta caggactagg ctttaatcaa gaacagatga cttatttgat ttcaattgat
    19621 atctttatgt gtggattagc gacattattg caattgacag ttaaccgatt tttcggaatt
    19681 ggcttgcctg tcgttttagg atgtgcgatc caagcagtag ctccactgat cttgatcgga
    19741 accaatgaag gagtaggagc tatctacgga tctattatcg cttctgggat ctttgttgtg
    19801 ttagtttctg gtgtcttttc taaaattaaa aaactattcc ctcctatagt aacaggaacg
    19861 gttatcaccg ttattggttt gactttgatt cctgtagcaa tcgagaaaat gggtggaggt
    19921 aatgcaacag cagctgattt tggtgacaaa acaaatctgc tacttgcatt tgtgacgatt
    19981 ctcctaattg taggcgtgca aatgctggca aaagggttca ttcgttcaat cgcggtattg
    20041 attggtttag tcggaggaag catcttagct gctttcatgg gaatgattga tctgagcgcg
    20101 attggagatg cgccgttgtt ccatgttcca caaccctttt attttggtaa accaactttc
    20161 gacgtatggt cgattttact aatgatcatc atttccatgg tcagcatggt agaatcaaca
    20221 ggtgtatatt ttgcacttgg tgacattact gggaaaaatg ttggagaaga agaattgaaa
    20281 aagggatatc gtgcagaagg acttgccgtt atcttaggcg gtattttcaa tacattccct
    20341 tatactggct tttctcaaaa tgtcggattg gttcaacttt cgggaatcaa aacaagaaaa
    20401 cccatctatt tctctgcatt cttcttgatt gttttaggat tattccctaa aatcggtgcg
    20461 ttagcacaaa tcatccctga gccggtactt ggtggaggaa tgttagtcat gttcgggatg
    20521 gtggcagtcc aaggaatccg tatgctgttg gaagttgatt tcagcaatga taaaaatcta
    20581 ttgattgttg ctgtttctgt tggatttggg ctgggtttca atgttacgcc gacattattc
    20641 agtcaaatgc cgcaaacatt gcaaatgttc actggaaatg gcatcgtaat gagtagcatt
    20701 acggctatca tattgaattt gatctttaat ggattgaaaa aagatgaaga aatattggaa
    20761 gaagccttca agaagtaata gagcaaaaga aaggttgtga aatcagcgtt ttgacctgat
    20821 tgttagagaa acaaacggaa aaatagttcc tcgtattttg ccgtttgttt gagctaatac
    20881 cgagggtcag ctgattgaac accgttgaag gaagaggtcg tgagcctgcc gttcatctcc
    20941 gagtcacaag gaacaatttc aaaaaaaagc ttctcatgtt tgttgaatat tgttccttgt
    21001 gaccgaggag ttggcaggag aacaccgttt atccataaaa aggaatgtga cctaactttt
    21061 gtcacattcc ttttttttat ctttaataaa cacgaacaat aaaatgtttt tctttgttaa
    21121 cattcgaaat ttctttgaca tcaaaaaaag tgattgttaa aatatgaatg tagaaaaact
    21181 gggaggaaaa acaatgacag caaaaatctc tgtgattatg gggagtactt cagattggga
    21241 aacaatgaaa gaagcatgtc aaatattgga tgaattcggt gtgtcttatg aaaaaaaggt
    21301 cgtttctgct catcgaaccc cagatttgat gtttgaatat gcacaaaacg ctcgaaaaaa
    21361 aggaatcaaa atcattattg caggagcagg gggagccgcg catctgccgg gtatggtcgc
    21421 atcaaaaaca actttgccgg ttatcggtgt accggtccaa tcacgaacat taaatggttt
    21481 ggattcgctt ctttcgattg tccaaatgcc tggaggagta cctgtagcta ctacagcaat
    21541 cggaaaagcg ggagcaatta atgctggact acttgcgata cagatgcttt ccatgtatga
    21601 tttagaattg gaggctaaat tggctgaaag acgttcaatg ttggctaaaa ctgttataga
    21661 aagtagtgat caacttggtt aaacctttgt tgcctggttc aacgatcggg atcgtcggtg
    21721 gcggtcaatt aggtcgtatg atggcgatta gtgcaaaaga aatgggcttt aaagtaggtg
    21781 tgctggatcc tgtgagcgat tgtccagccg cacaagttgc agattggcat attgaaagtg
    21841 tctatgatga cacctttgcg ctgaaagagc ttgccagaag aacagatgtc attacctatg
    21901 aattcgaaaa cgtcagtgta gaggcgttaa atgcgatttt acccatgtca tttattcctc
    21961 aagggacaga cttgcttgcg attacgcaag accgcctgtt agaaaaatct tttttggaaa
    22021 cgaataacat cgtgattgct ccttatgcaa cgatcgtcag tccgacggat atccaagatg
    22081 cgattgacgg gatcggctat ccttgtgttt tgaaaaccac tcgaggcggc tatgatggca
    22141 aggggcagta tgttttgaaa agtcgggcgg atctagctcc tgccatggac cttttaagag
    22201 aaggaacctg tgttttagaa gcatggatcc cgtttgaaaa agagatttcg atcatggtgg
    22261 caggaaatgg tcaaggagat ttcacgactt ttccggtcgt ggaaaacatc caccacaaca
    22321 atattcttca tgaaacgatt gctccagccg cgattgatca ggatgtgatc gaggaggctg
    22381 agcgtattgc ccgtgtgatt gcagaagcag tgagcctttc aggtgtttta ggggtagaaa
    22441 tgtttttaac aaaaacaggc gggctatatg tgaatgaatt agctcctcgt cctcacaatt
    22501 ctggacatta ttctattgaa gcttgttcta tgagtcagtt cgatgcccat attcgaggta
    22561 tttgtggttg gccattagga gaaaaagtac gtttgataag caatgcagta atggtcaatg
    22621 ttttgggaga acaactgctg gaaagctatc gcttgattga gaataagcca gactggcaat
    22681 tccatttcta tggaaaaaaa caagcaaaaa aaggtcgcaa gatgggacat atcactatct
    22741 tgacagaaga catccagcaa acgctggaag aaatcaaaca aacgaatatt tgggattaga
    22801 ggagaaaaaa catgttagaa cgttatacac gccctgaaat ggggaatatc tggacggaag
    22861 agaacaaata ccgtgcgtgg ctcgaagtcg agattttagc agatgaagct tgggcagaac
    22921 ttggtgaaat cccgcaagaa gatgtgaaaa aaattcgtga gcatgcatca tttgatatcg
    22981 atcggatctt ggaaatcgaa aaattaacac gccatgatgt cgttgcgttt acacgagcgg
    23041 tttctgaaac attgggagaa gaaagaaaat gggtccatta tggtctaaca agtacggatg
    23101 tcgtagatac tgcttatggc tatctcatca agcaagcaaa tgatatttta cgggaagact
    23161 tgaaacgttt tacggaaatc attggcgaaa aagcaaaaga acacaaatat acagtgatga
    23221 tgggtcggac acacggtgtc catgctgaac caactacttt tggattgaaa cttgccacat
    23281 ggtattcaga aatgaaacgg aacatcgaac gatttgaaca tgcggcaaaa ggagtagaag
    23341 caggaaaaat ctcaggagca gtgggaacgt tcgcaaatat cccgccattt attgaagagt
    23401 atgtatgtaa acaattgggg atccgtccac aagaaatttc tactcaagtt ctccctcggg
    23461 atctacatgc tgaatatttc tcagcgatgg cgcttattgc tacaagtatt gaacgttttg
    23521 caacagagat acgcggattg caaaaatccg aaacacgtga agtagaagaa ttttttgcga
    23581 aaggtcaaaa aggttcttct gctatgccgc acaaaaggaa tccgatcggt tcagaaaata
    23641 tggctggtct agccagagtg gtgcgcggac acatggtcac tgcctatgaa aatgtcacat
    23701 tatggcatga aagagatatt tctcattctt cagcagaacg aatcatcttg cctgatacca
    23761 cgattttatt gaattacatg ttaaatcgtt ttggcaatat cgttaaaaat ctaacggtct
    23821 ttcctgaaaa catgaagcgg aatatgtctg caacatttgg tttgatttat agccagcgcg
    23881 ttatgctgaa attgatcgat aagggaatga caagagaaga agcttatgat cttgttcagc
    23941 caaaaactgc gtacgcttgg gacaaccaag tgcagttccg cccgctgtta gaagaagatc
    24001 caaccatcac atcggtatta tctaaagaag aaatagacga tgcctttgat tatcatcatc
    24061 atttgaaaaa cgttgacgtg atattcaaac gagtaggttt ggaataaaga agagtgagtt
    24121 gcaaaattca attaaaatag aatagcaaaa ataaccaaaa aagaagcaga tccgttagcg
    24181 gttctgcttc ttttagttta ttatattttc ttatcaataa taggaacctg attttaaaag
    24241 cggctacctc tttaccgtgc ctctttacat acaaaaaata gaggaattaa gcgtaaatgg
    24301 cttggctatt ataactaaaa tgctggttat ggagatgttg cagcaagttt tcgagaaaag
    24361 cgtattccat ctcttgtcca ttgaataaga tctggatacg ataatgattt ttgaaacgct
    24421 tctgttcatg aatctgactg attgaaagtt cgcctttgtg ttctgtctga taaagaaaag
    24481 tccttttacg gcattcagca cagcgcttgg ttgctcggcg gatttttcgg aatgtataaa
    24541 aactagtatc tttattgcat aaataaacgt ctttgatcga cagcaaatca ttatatagac
    24601 aggaacgttc ttcagctgtt aattctgata aaaaaagctg accttgtttt attgcatctt
    24661 ccatcggcaa gtgcctcctc gaacccatta tccaaatttt tagttaattc taatatacca
    24721 tcattaagta taaatgtaaa cgcttgttga tggtataaga ttttcgtttc tgataaaaca
    24781 cgaacattaa aagaatctaa tgccttaaat gtacatattt ttgttgattt ataaaaagat
    24841 gattgctaaa atttaaagtg aaaaaacgaa caaaaaacga tgtatttgct tttaatgtaa
    24901 ataaaaagga gagacgtaca tggagaagaa agaacttttg tatgaaggaa aagccaaaaa
    24961 aatatatctt acttctgatg agaacttgat ttggatagaa tatttagatc aagcaactgc
    25021 gttgaatggc gcgaggaaag accagataag tggaaaagga gaattgaata atcaaatcac
    25081 aagtgcaatt ttccgttatc tgactcaaag aggaataaaa aatcatttta ttgaacaatt
    25141 gtctaaaacc gaacaattag caaaaaaagt acaaattatc cctctggaag tggttatccg
    25201 aaatattgca gcaggaagtt tctcaaaacg tctaggtgtt cccgaaggta aagaattcat
    25261 caaaccaatc attgaattct actataagga tgaccagttg gatgatccat ttatcaatga
    25321 agagcatatc tatttcttac aacttgcaaa gccggaagaa atcagtgagc tgaaagccca
    25381 agcgttggct attaatgaaa ttttgaccga attatttgct aaggtcggtt tggttttagt
    25441 agatttcaag ctagaatttg gccggtcgaa tggacaaatt ttgcttgcgg atgaagtaac
    25501 accggatact tgccgattat gggatgaacg tacgaatgaa catatggaca aagacgttta
    25561 ccgcagagat ctaggtgatt tgatcccagt ctatcaagaa gtatataacc gtctatcgga
    25621 actaactgaa gaaaaggagc gagtatgatg tatttcgtaa aagtatatgt aacctataaa
    25681 gattcagtgt tagatcctca aggggaggca gtaaaaaaag cagtccatcg tatgggatat
    25741 gaaacaatcg aagatatccg aataggaaaa tactttgaga tacaacttgc tgatgcagaa
    25801 gaaccaatcg atacagtgat tgaaacgatt tgtgacaagc tgcttgcaaa tgtaaatatg
    25861 gaaacatacc gatatgaaat caaaaaaatg gaggaagtat aacatgaaat ttgcggtcat
    25921 cgtttttcca ggttcaaatt gtgacgcaga tatgttatgg gcagttcgcg agatcatggg
    25981 cgctgatgcc gaatttgtac ggcatgatga gtcctcttta gaaggatttg atggggtttt
    26041 actgccggga ggattctcct atggtgacta tttgagatgc ggtgcgattg ctcggttttc
    26101 tacaatcatg gaagaagtga ttcgttttgc tgaagaaggg aaaccagtat tcggtacatg
    26161 caatggattc cagatattga cagaagcagg acttttgcca ggtgtactga gacgaaatga
    26221 gtctttgcat tttatttgca aaactgtacc attgaaagtc aaccccaaga cggcctttac
    26281 ttcagagtat gaagccgacg aaataatcta tcttccagtt gctcatgggg aaggaaatta
    26341 tttctgtgac gaagaaacac ttcaagaact aaaaaaaaat aatcagattg cctttactta
    26401 taatgacaac atcaacggca gcgtggaaaa cattgcaggg attacgaaca aagcagggaa
    26461 tgtgttagga atgatgcctc atccagaacg tgcagtagaa gacttgctag gatcagaaga
    26521 tgggaaacgg ttctttgctt ccattttaaa aaacttcgga aaggtgaccg tatagatgaa
    26581 aacattgaaa gaaccgacag ctgaagagat caagaaaaaa agaatctatg ccgaatgggg
    26641 attgactgat gaagaatatc gaatgatcga agaagatatc ttaggaagaa tgcctaatta
    26701 tacggaaact ggactgtttt ctgttatgtg gagcgaacat tgttcctata aaaattctaa
    26761 accggttcta cgcaaatttc ctactagtgg acctcatgtg ttgcaaggcc ctggagaagg
    26821 cgcaggcatc gtcgacatcg gtgatggact agcagttgtc ttcaaagcag aaagtcacaa
    26881 tcatccttca gccgtggagc cttatgaagg agctgcgaca ggagttggcg gaatcatcag
    26941 agatattttc agcatggggg ctcgtccaat tgcgttgcta gattcattga ggttcggcga
    27001 attagataat ccaagaacga aatatttact agaagaagta gttgcaggta tcggcggata
    27061 cggcaattgt atcggcatac cgactgtagg cggcgaaatt gcatttgatc cttgttatga
    27121 aggaaaccca ttagtcaatg caatgtgcgt aggtttgatt gaacacaaac atatccagaa
    27181 agggcaagcc gctggcgtcg gtaattcgat catgtatgta ggtgcaaaaa caggtcgcga
    27241 cggtatccac ggagcaactt ttgcctcaga agaatttaat caagaagaag aacagcaacg
    27301 ttctgctgta caagtaggcg atccttttat ggaaaagctg ctgcttgaag cgtgtttgga
    27361 attaatcttg catcattcgg attctctaat aggtatacag gatatgggag cagcaggact
    27421 tgtttcatct agtgcggaaa tggcttcaaa agctggctca ggacttatat tgacattaga
    27481 tgaagtccca caaagagaaa ctgggatgac accttatgaa atgatgttat cggaatcaca
    27541 agaacggatg ttgatttgtg ttaaatcagg agaggaagaa aaaatccaag aactatttca
    27601 gaagtatgat ctagatgcag tcactatcgg taaagtaaca gacgacgggc aatatcggct
    27661 atatcatcaa ggagaagaag tagcaaatct gccagtcgat gcattagctg aagatgcacc
    27721 agtttatcat aaagaaatga aagaaccagc tcgaatcgta gaatttcagc agttagctcc
    27781 ttatcagcca gttatcgaag aacctggaca agtattgctt gatctgctgc agcaaccgac
    27841 gattgcttca aaacgttcaa tctacgaaac ttatgattca caagtacaaa ccaatacagt
    27901 tgtccgtcca ggaagcgatg ctgctgtgat gagggttaga gggacgaata aagcgttagc
    27961 tatgaccaca gactgcaacg cgcgctatct gtatttggat ccaaaaatcg gaggacaaat
    28021 agcagtggca gaagctgcta gaaatattat tgctagcggt ggtaagccct tggcgatcac
    28081 cgactgcctg aactatggtt ctcctgataa accagaagtt ttttgggaat tatcgacttc
    28141 agctgacgga attgctgctg cgtgtgaaac gttaggaact cctgttattt caggaaatgt
    28201 ctctttatat aatgaaacgg atggtcaagc aatctatcca acaccaatga ttggtatggt
    28261 cggattgatt gaggatcata aacatatcac tactcaggaa ttcaaaaaat caggggatct
    28321 aatctatata ttaggtaaga cgtttgcaga ttttgatggc agtgaattac aaaagatgca
    28381 gcttggaaga atcgaaggtg tgatccgaaa ctttgatcta tccattgaaa agagaaacca
    28441 agagttagta ttgacagcca tccaaaacgg attgattgaa agtgctcatg actgttcaga
    28501 aggtggcttg gcaattgcac tggcagaatc agctttcaaa catcagctag ggatatctgt
    28561 acagtttgaa ttgtcctctg ctcaattatt tgcagaaact caatcaagat ttgtgttgac
    28621 agtcgcaccg gaaaataaga cacgattcga agaaatgatg ggagatgctg ctgttttagc
    28681 aggtaaggtg acggatgaag cgataatcga aatttcagca actgacggtc agatcaaaat
    28741 agaaactgca gtagcaagaa agtgctggga ggatgcaatc gtatgtctta tgaagtgaag
    28801 agtttaaatg aagaatgcgg gatcttcggt gtttggggtc atccagacgc cgctagtgtg
    28861 gcatacttcg ggctccatag tcttcagcat cgaggacaag aaggtgcagg tatcgtgtcc
    28921 aatgatcgag gaaagcttaa ggggcatcga gatttggggc tgctttctga ggtattcaaa
    28981 gatcaaagaa aattgcagca gttaacagga gaagctgcaa tcggacatgt acgttatgca
    29041 acagcaggca atggaagtgt ggacaatatt cagccttttc tattcaaatt tcatgatcag
    29101 tcgatcggct tagcgcataa tggtaacttg atcaatgctc gttcccttag aaaagagtta
    29161 gaggaaaatg gggcgatctt ccattctaac tccgatacgg agatattgat gcatttgatc
    29221 cgacgaagtg aagaaacaga ttttttaaat aaattaaaaa gtgctttgaa tgaagtcaaa
    29281 ggtggctttg cctatttatt aatgacagaa acggcaatga ttgccgcttt ggacccaaat
    29341 ggttttcgtc cattagcaat cggacaaatg aaaaacggag catacgtgat tgcctcggaa
    29401 acttgtgcat tagaagttat tggggcacgt tttataagag atgtccagcc tggtgagata
    29461 gtgatcatca atgacgaagg aatccagatt gattgcttta cgaaggacac gcagctttct
    29521 atttgttcga tggaatatat ctattttgcc agaccagatt caaacatcgc agggatcaat
    29581 gtgcatactg cacggaaaaa tatgggacgg aatttagcga aagaatcgcc agtaaaagca
    29641 gatatggtcg ttggcgtacc taattcttct ttgtcagccg caagcggtta tgcggaagct
    29701 agtggaatcc cttatgaaat cggattagta aaaaatcaat acattgcccg aacatttatc
    29761 cagccaacgc ctgagctaag agagcaaggt gttcgaatga aattatctgc tgtacgagga
    29821 gtagtggaag gtaaaaaggt gattatggtc gatgattcaa ttgttcgggg tacgacaagc
    29881 agacggatcg tccatctttt gaaagaagca ggggcaaaag aagtccatgt gaggattgct
    29941 tctcctcctt tgaaatatcc atgtttttac gggattgata ttcaaacgcg taaagaactc
    30001 attgcggcaa atcacacgat tgatgaaatc aaagaatgta tcggtgcaga ttctttaggt
    30061 tttttaagtg aagagggtct gatcgaatcg atcggtctgg aaaaagatgc tccgtattca
    30121 ggattgtgta tggcttattt caacggagat tatccgacgc cgttatatga ctatgaagaa
    30181 aaatatcagg aatcactaaa agaaaaagta tcgttctttt aaaagcagac caagaaggga
    30241 gtatatgaat tcaatggcaa atgcatacgc aaaagctggc gtagatgtcg aagctggata
    30301 tgaagtagta gaacggatca agaagcatgt caaaaaaacg gaacgtttag gtatgatggg
    30361 cgctatagga ggattcggcg gttgttttga tttatcggtc tttcaattaa aagagccagt
    30421 ccttgtctcg gggaccgatg gcgtggggac gaaattagca gtggcaatcg aagcagacaa
    30481 acacgataca atcgggatcg actgcgtagc gatgtgtgtc aatgatatct tagcacaagg
    30541 cgcagagccg ttatatttct tagactactt agcaacgggg aaaaatgatc cgttacgttt
    30601 ggaaaatgta gtaaaaggtg tagcagatgg atgtgttcaa ggaaatatgg cgctgattgg
    30661 cggagaaaca gctgaaatgc ctggtatgta ctcaggtaat gattatgatc tagccggatt
    30721 tgctgtaggg atagctgaaa aatctcaatt attgacagga gaaacgatca acccaggaga
    30781 tgtgttgctt ggaatagcat ctagcgggat tcattcaaat gggtactctc ttgttcgcaa
    30841 aattttcttt gagcagcagc agttttcttt atcatcaaaa ctgccagaat tgaaaaaaac
    30901 acttggcgaa gaattgcttg agccaacgaa aatctatgtt tcttccttgc ttccgctgat
    30961 caaagaaaag aaaataaaag gagctgcaca tatcactggt ggagggtttg tagaaaatat
    31021 cccgcgaatg ttaccagaat ctatggctgc acaaatcaaa cttggctcat ggccgattct
    31081 tccaatattc ggctgtttac aaaaatatgg tgaaattcct gagatggaga tgtatgaaat
    31141 cttcaatatg ggcatcggta tggtcctggc agtcgaagaa tcgaaagtaa aagaaatagt
    31201 acacgaaatt gagcaagctg gcgaaaaagt ttatgaaatc gggaaagtga tcgccaaaga
    31261 aaaacatcgt gtggtgttta taggagaaga acgatgagaa tcgctgtttt tgcttctgga
    31321 aacggaagca actttcaagc gctagccgat tatctatcga aaaaaggatt ggaatcttcg
    31381 attgactggt tgttttgtga tcagcctgaa gcatatgttt tgaaacgtgc aactgcgttg
    31441 agtgtaccgg cagattgttt ttcacctaaa gaatttgatt caaaaaaaga gtatgaagaa
    31501 gcaattttgc ataaattgaa agaaaaaaag atcgatttga tcgtattagc tggatatatg
    31561 cgaatcattg gaccggtatt gctgaaaaat tatgataaaa gaatcatcaa tatccatccg
    31621 tctcttttac ctgccttccc tggtttgcat ggcatacgtg atgcctttga agctggagta
    31681 aaagaaacgg gtgtcacaat tcattatatc gaccaaggtg tagatactgg accaatcatc
    31741 cggcaggaaa aagtgagaat tgaacaggag gacacgttcg attctttaga agaaaaaatc
    31801 catagagtag agcatcgaat ctatccagaa gtgattagtg aaattattga aaatggaggc
    31861 tatctgaaat gacaagagca ttgatcagtg tatcagataa aactgggatt gtagcattcg
    31921 cccaaggttt gattgaacaa ggagtagaaa tcatttcaac aggaggaaca aagcatgcgt
    31981 tggataaagc tgggatcaaa acgctttcga tcgaagaagt gacagggttt cctgaaatga
    32041 tggatggccg tgtcaaaaca cttcatccta aaatccatgg tggtctttta ggaagacgcg
    32101 atctccctag ccatatggag gcaatggaaa cggaaaatat ccaaccaatc gactttgttt
    32161 gtgtcaattt atatcctttc aaagaaacga ttatgaaacc agatgtcgaa atagcagaag
    32221 ctattgaaaa cattgatatc ggtggtccta gtatgctccg tagtgcagca aaaaaccatg
    32281 aatttgtcac ctctgttgtt gatccatctg actatgaaga agtactttca gaattacgtc
    32341 agtatgggca tacttcactt tctttcagac aaaaactagc ggcaaaagtc tttcgtcata
    32401 ctgcggcata tgatgcactg atcgctcaat acttaacgga ttgggtggaa gaaaaagaac
    32461 cggaaaaatt gacacttacg tatgaacgaa aacaaacgct tcgttacggt gaaaatagcc
    32521 atcaagccgc tgccttttat cagtccgctc ttctggaaaa atattcgatc gcagcagcaa
    32581 aacaactcca tgggaaagaa ctttcttata ataatatccg agatgcagat gctgcgctac
    32641 ggatcatgaa ggaatatgac gagccaacgg tagtcgcctt gaaacacatg aatccttgtg
    32701 gtattgggac agcatctacg attttagaag cgtgggaagc agcgtatgaa gcagatcctg
    32761 tttcaatctt tggtgggatc atcgttttga atcgagaagt agatctgcca acagcagaac
    32821 agatgcataa gctgtttcta gaaatcatca ttgccccttc ttatacacaa gaagctttgg
    32881 aattactgca aaaaaagaaa aacatccgat tactgacagt agactttgcg caggaagaaa
    32941 atcaaaatcg agaagaaacg gtctcagtct taggtggact tcttgtacaa gatcaagatc
    33001 tggcaataga aaatgaagaa gagtggaaag tcgtgacaaa acgtaagccg actccagcag
    33061 aagcaaaagc attagtgttt gcttggagag ctgtcaaaca tgtgaaatct aatgcgatcg
    33121 tactggcaaa cgaacgtcag acagtaggaa tcggagccgg gcagatgaat cgagtcggtt
    33181 cagtaaaaat tgcgattgaa caagcgcaag agaaaatgga aggtgcagtt ttagctagcg
    33241 atgcttattt tccaatggat gattcggttg aatatgcagc caaacatggt ataaaagcaa
    33301 tcattcagcc aggaggaagc atcaaagacc aagattctat tgatatggcg aataaatacg
    33361 atctagcaat ggtctttact gatgttcggc attttagaca ttagagagga gtcggataaa
    33421 tgaacatttt ggtgatcggt gcaggtggtc gtgaacatgc aataagtaaa aaacttttag
    33481 ccagtccgaa ggtggatacg gtgtattgtg cgccaggaaa tccggggatg acaaaagatg
    33541 gtatcaattg tgtcgctatc agtgagcgag atcattcagc gttaatccat tttgttaaga
    33601 aagaggctat tgattggagt atcgttggtc ctgaagttcc tttgttaaat gggatcgtcg
    33661 atgattttat ggcggaagga ttgaaaatct tcggtccgac aaaagcagca gctctgatcg
    33721 aaggttcgaa aagttttgct aaacaattaa tggatactta tcaaatcccg acagctaaat
    33781 ccagaagttt tgaatgttac gaaaaagcag cagcgtatat caaagaaaat ggtgcgccaa
    33841 tcgtagtgaa agcggacggc ttagcagctg ggaaaggtgt agtcgtggct accacagtga
    33901 aggaagcgct ggatgcggca gaacaaatgt tgaaccagca tcgatttggg gatagtagca
    33961 aaaaaatcgt cgtagaagag tttttggcag gagaagaatt ctcccttttg gcgtttgtcc
    34021 gtaataccaa ggtgtatccg atggtcattt cgcaagatca taagcgggca tttgataacg
    34081 ataaagggcc aaatacaggc ggaatgggtg cttattcacc agtaccgcaa atttcaaaaa
    34141 agatgattga aaaagctgtg gaagaagttt taaaaccagc ggctttggga atgaaagaac
    34201 taggacgctc tttcacgggt gttttatatg cgggtttgat tgctacgaag gaaggaccaa
    34261 aagtgatcga attcaatgcg cgtttcggtg atcctgaaac gcaagtcgtt ctttctcggt
    34321 tgaagagtga ctttgcgcaa gtaatcgatg atttattaga gaatcgtaca gtagagttaa
    34381 agtggcagca agaaggatac aatgtaggcg ttgtggttgc agctgccggt taccccgaag
    34441 aatatgagac aggaatggtt ctaccagatt ttctggaaga agagctttct gtttactatg
    34501 ctggagtttc ctcaaataac ggcaaactag ttggagcagg aggacgtctt tatttagtag
    34561 aagcatacgg tgagacgatc aaagaagcac agaaaaaaat ttacaccgcg ttaatggata
    34621 aaacgacaga tggaacattt tatcgtacag atattggaga aaaagctgcc aatagcgaac
    34681 tgatcgtgaa gtaaatcagc tgcttccatt tataaaaaat acaacaaaca taatcggaag
    34741 cgccgatttt agtgaagcct tttcataaag agtgtggtac acttttgttg tgtatataaa
    34801 taaggaggag ttcagatgaa aaaaattcaa ttaggtactt ctgatcagaa agcagctgca
    34861 gtgatcttag gctgtatgcg tatcaacaat gcggaagatc cagtaaaagt gattgagaca
    34921 gcttatgaaa acggcattga tttttttgac catgcagata tttatggaaa cggtgaatgt
    34981 gaagaaatct ttgcagatgc tctagcaaaa acatcaatca aacgagaaga tctttttatt
    35041 caaacaaaat gtggaatcgt tccaggtgtg atgtttgatt tttctaaaaa acatatcatt
    35101 gaatctgtag aaaaaagttt gacccgtctg aaaatggatt atgtggatgc tttacttctt
    35161 catcgtccag atacattggt agaaccagag gaagtagctg aagcgtttga tgaattggaa
    35221 cggtcaggaa aagttcgtta ttttggcgtc agcaatcaaa aaccaatgca aatcgaattg
    35281 ctgaaaaaat atgttcgtca accattagta gctaatcagc tgcaatttgg cttgaaacat
    35341 actggaatga tcgatcaagg gattcatgtc aatatgacag atgaagcaag tatcgatcat
    35401 gacggtagtg tacttgatta ttcccgattg catgatatga cgatccaagc atggtcacca
    35461 taccagtatg gattttttga aggtgtattt atcgggaacg aaaaattccc tgaattaaac
    35521 caaacattag aagaactagc agctaaatat caaacgacac caactggctt ggcttctgct
    35581 tggattttac gccatccagc caatatgcaa gtgatcgccg gtacgatgaa cgtagggcga
    35641 atcaaggaaa tcgctcaagc ttcagaaatt aatatgtctc gggaagattg gtacgaattg
    35701 taccgtgctg ctggaaacat tttaccttaa agattgtcat aagcaggtca aaagggctat
    35761 aaattaaaaa gacagaataa cttgaaagct gttttcagct tcgagatatt ctgtcttttt
    35821 ttattgcaaa tggaaagagt atcattgtac ggatttcgtt tattagttat ttttgttttt
    35881 aaggaaaagc tgtgtccgat attcttttgg agaaatccct tgttttttct taaataaata
    35941 actgaaatag ttagggtcat tataaccaat ttcaaaagca atatctgaaa tacgatagtc
    36001 tgattgctgc aataattttt ttgctaattc gattctttgt tctgtcagat aatcgatgaa
    36061 tgtttggtta aacgattgag aaaaaatcgt actgaaatga gagggactta atgcaacttg
    36121 ttgagcgacc caagttaagg acatatctgg atcggtaaaa ttttctttga taaaatccaa
    36181 tgcatggttc aaaattggct gatatttgtt ccttgaaggc tgtgagggca gagcagttaa
    36241 gaaagaaatg aacatataaa ctgtcgaacg gtaaagcgag tcgtcggcgc tgattgctaa
    36301 gcgttgactg gtggatgtgc aatgaaccat ttcttgcggg attttctttt ctttcaggca
    36361 gacatgctgg agtagcttag tcaagacaaa taaacgatat aatcgtgttc gagttggtgt
    36421 gccttgtggt ttcgtcaagt gttcgatgta cttagatact tcgtttttcg ataatgaaga
    36481 caaatcaagt gagaggtcaa agtaaaagac agaatcaact ggaagtttcg cttcttgaat
    36541 caaatcctca tagctgataa tttgttcttt aagggaactg gcggtttcaa gtatcatctg
    36601 cgtgatctca aaagatgcag gtaattcgct gattctttct gcgacaggac caatggcgac
    36661 agaaaaatta tttttttgtc ccttttctaa ttcatggacc aacgtttggg ctacctgata
    36721 actggtctcg atcacatttt cttcaagaga atcaactatt aaaaatttga tgaatcgaga
    36781 ggaaacagca gagaagatca cttgttgatt ctccctaaat aaaaacttca aataatcact
    36841 gagatgagcg taatcttcaa agtctgtact gacttggttt gctgctaata atacggtcat
    36901 ttttttccct gctaaagagc gattgaaaga ttcagcaagt ttcaatgttt cactgatcgt
    36961 aagtgagcct ttgaacaaac cgttcaagaa atgatttttt ttcaattcga aaacaagatc
    37021 agaagaagtg tgcgtttctt tttgctgcaa atctaaagca gttactgctt tttctattgc
    37081 ttgtttcaat tcgctacttt taatcggttt aagcagataa tcatctgcct gtacctggat
    37141 tgctcctttc atataatcga aatcgtcgaa tccactaata aatatgagac gagtccaagg
    37201 aagtagtttc cttgcttctt ttgaaaaagt caacccgtcc atgaaaggca tttggatatc
    37261 agtcagaatg atgtcgggct gtaagtcgat aattgaagac agtgccatct caccatctcc
    37321 agcttcgccg acataagtga ttggataata agaaccaagg acagttattt gttcccgcaa
    37381 gttattgcga atgaggtatt cgtcttcgat gataaaaact ttatacatgt ttgtttcctc
    37441 caattgtttt gggagataga gggatcatca gtgtgacagt tgttccttct cggtatttgc
    37501 tgtcaatcgt gataggtttt tgatcatttc cgtaataaag aaggagacgc ttgtttacgt
    37561 tgtaaagtcc gtagccgata ctgaaatctg aatcgatccc tttggctaac tctttacgta
    37621 tttctgccag tttttctgca gggatgccaa tcccgttgtc tataactttg aattggatca
    37681 tctcatcaat tttgcatgtg gtaatacgaa taaatccttt gtgacggacg tacttcgttc
    37741 catggtatac agcattttca atcaatggct gcaagatcat ttttaaaata gaaacaggaa
    37801 aaagtgtttc tggtatatcg atcttatagt caagcatatg gccgtatctt atttttaaga
    37861 tgactagata atcattgata tggctaattt ctttttcaag aggaatccag tcttgtcctt
    37921 tgcttaaaga cgtacgaaaa aagccggaaa gtgcatgggt catagtcaga gcttcttcaa
    37981 agcagtcttg ttcgatcaaa gaaataatgg catccaaact gttataaata aaatggggag
    38041 taatctgagc ttgcaataca cgaacttcac tttgtgctaa atggtattgt ttcaaagcgt
    38101 tctcccgtaa agactggttt agatcttccg ccattttatt catgctagcg gtaagtgttt
    38161 gtaattccag tgtttccggc agggttaaac gaaagccaag gttccctgaa gaaagttgtt
    38221 ctgacatctc gatcagctga ttgatcggtt cttggacctt tctctctaaa aagcgacgat
    38281 tctttttcgc atagaaaaaa atggtactaa gaactccaag ttccactatt gttaatagaa
    38341 tgagcgagcg gacaagtgtc gtattttttt tgcttgctaa gtcgatctcc acacgaacaa
    38401 aatcttgtaa gatatctgac agcagttgag tgacagaatc tacctgattg atgatctctt
    38461 cattggtatc cataaaatct tttgccaaga tattattgat cagctcgttt tgataatctt
    38521 ccagtgtatc gatcgtacgt agcgatacat ctaacgtact aatttcttta agtgtatttg
    38581 tattattttt gctctctcga atatcattcc taagttcttc taggatattt tcctcattga
    38641 actcatctac ggttacttgg ccagaaatca agtcccacat actttccaaa actttttgat
    38701 caaccttcaa agcaattgca tctgcttttt ggatatttgc gatcgtctgt tgataaatca
    38761 aaagctgttg cgaatataac agtatgctca gtagcaaagg gataacagca aatagaagca
    38821 tggctttgtt tgcttgggca aaaatctgtt ttatgcttaa attttttgtc atgatacttc
    38881 tccagtcaat aatttctggt tgggatgtta gctatattct gctgggaaaa gacagtctcg
    38941 gaaataaaaa tatctttagg taatgttttt cctgtttgaa tatttccaaa ataacgagta
    39001 acggtattgg ctacatacca gccgacatca ggtttacact cgactacaca attgacgatt
    39061 cctgagcgta gcttctcgat catatcttct tgtccatcaa tagttacaat gatcaaatct
    39121 tctccaggtt tgattgatgt ttcttttatc gcagctaaag caccgtgagt catttcatca
    39181 ctatggctgt ataatacatc gatttcggat aaatcatttg tttctaaata tcgtttcatc
    39241 tggtcttttc ctttcatccg aataaaatca ccgacaattc ggtgaacaat agtcatcgga
    39301 ggtttatggt cgatggttcc atcttttttt agggttggtt tacgattgat tgattccaaa
    39361 aatccttcgg agcgtagctt ggtcggtgtg gagttctctg aaccagcaag ttctaggatt
    39421 ctgatttcct gtttctttga atttgtgaag tgattgctga caaataaacc cgcccgattt
    39481 ccttctgctt tgaaactggg gccaatgtgg gtgacgaaaa gctcgggatc attcgtttga
    39541 atatgtctgt cgacaagtat gacagggatc cctgctcgct ttgcttcttt taatacagag
    39601 tcccacccgg attcttgcaa aggagtaaaa atgatcatat ccactttata ggcaatgaac
    39661 gtccgtatat cttggatttg ccgttcctga ttcatgtagc catttcggta gagaacttgg
    39721 taatcttctt tttcaagcgc agtgataatt gattcagtat gctttttccg ccagctactc
    39781 tccgttcctg attgcgaaaa accaacgact agtgggtcag gtgcttcctt tgtattaggt
    39841 gtacacccca tcatttggta agcgattaca acaagggcta aaaaaaataa aacacgtttc
    39901 actttaaatc actccatctg gatttgttag tactatctta tcagataaaa gggaaaatca
    39961 aagtcaaaag gaatcaatcg aaaaaaatcc aagaaactta aaaaaatcca agacaaaaaa
    40021 agtacaccta aaaaatttaa agtaaatccc ataaaaaaat caatgttaac gctttcttta
    40081 ttagctatac tcggtgtatc aattgaaatg ggggataaaa aatgaaaaac aaatttagaa
    40141 agattgttgg gactatcgct attttgaccg caagcattct attaagtgcg tgtggcaatt
    40201 caggatctgc agatgatagt acggggtatg taggaatcgc tatgccgacc aaatcagcag
    40261 aaagatggat cgctgatggg aacaacatgg tctcggaatt agaaaaactg ggttataaaa
    40321 cagatcttca atatggagaa gataaagtag aaaatcaagt agcacaaata gaaaatatga
    40381 ttacaaaagg ggtagataca cttgtgattg cttctatcga tggttcggct ttaactgatg
    40441 tgttagctaa agcaaaagaa gcagatatca aggtgatcgc ctatgatcgt ttactgatga
    40501 attcagaaaa tgtcgattat tacgcgactt ttgataattt tggagtaggt gtatcgcaag
    40561 cagcttatat tgaagagcac ttaggattga aagaaggaaa aggaccattc acgattgaat
    40621 tatttggtgg ctcgcctgac gacaacaacg cactaatcaa ttacaacgga gtgatgtctg
    40681 ttttacaacc atatatggac aacggtcagt tggtcgtgcc atctggacag acgagtttca
    40741 gtcaaatcgc tacgcttcgc tgggatggtt ctacggcgca agcaaggatg gataatctgt
    40801 taagcgcaaa ttatactgac caaacattgg atgctgtttt gtcgccatat gatccaataa
    40861 gtttaggaat catttcgtca ttaaaaggcg tggggtatgg gagtgaaagt aaacctttac
    40921 ctgtgattac tggacaagat gcgacagttg ctggcgttaa atcgatcatt gccggggaac
    40981 aaacacaaac gatctttaaa gacacacgta tcttagcaaa aaatacgatt gaaatgatca
    41041 aagcaatcag tgatggagaa gaggttccgg tcaatgatac tgaaacttat gataatgggg
    41101 tgaaaactgt ccctacgtat ttagcaaata cagtatctgt cgataaagat aattatcaag
    41161 cagagctgat cgacaccgat tattataaag aatccgatct gaaaaattaa gaggaaccaa
    41221 acaaggaact aaagagatct aagtcagaga gaggataaga attatggcgg attgtatttt
    41281 aaaaatggca gatatcacca aaaaattttc tggaatccga gcgttagatt gcgtgaatct
    41341 gatgattgaa cgtggggaaa tccatgcatt gtgcggagaa aacggagcgg gaaaatcaac
    41401 attgatgaat gtcttgagcg gcttgtatcc atatggctcc tatgacgggg aaatctttta
    41461 tgatggaaag ctgtgtcaat ttaaagattt gaaagatagt gaagaaaaag gcatcgtcat
    41521 cattcatcaa gaattagcac taagtcctta tctttcaata aaagagaata tttttctcgg
    41581 aaatgaacaa gcagaaagag gcgtgattga ttgggaactg acagaaaaga aaacatatga
    41641 actgctgaat aaagtcggtt tgtctatcga cccgaatctt ctagtatccc agattggcgt
    41701 agggcaacag caactagttg aaatagctaa agcattatca aaatccgtcc gtctgttgat
    41761 tttggatgag cctaccgctg cattgaatga agaagaaagc gcgaatttgc tgagattgat
    41821 ccgagagttg aaagagcaag gtgtaacctc catcattatt tcacataagc tgaatgaaat
    41881 cgtggatatc gcagatcgaa tcaccatttt acgggatgga cagacgattg agacactaga
    41941 aaatgaaatg atcagcgaag agcatattat tcgcggaatg gtgggcagag atttagttga
    42001 tcgttatcct gaaagaaaac cggatattgg agaagtttat tttaaagtag aagactggac
    42061 ggtctatcac cctcttgatc acgagcgaat cataaacgac cacatcaact tgcatttgaa
    42121 aaaaggagag attgtaggaa tagctggact gatgggagcg ggcagaactg aattcgctat
    42181 gagtctattt ggacattctt atggaagcaa gatatccgga cgtatattaa aggaaggggc
    42241 agagatatca gttaagaccg ttccttcagc cattgcaaac ggactcgctt atgtctcgga
    42301 ggaccgaaaa tctttaggac tgaatctgct gatggatatt cgggaaaacg tgacgctgag
    42361 cagtttggat aaaataagtc aaaaaggagt attagataaa gaaaaagaag taactgaggc
    42421 agaaaatttc cggaaaaaga tgcgtataaa aacaaactcc atccatcaaa ttgtcagtag
    42481 tcttagtggt gggaatcaac aaaaagtcgt tttatccaag tggctgatga cacaacccga
    42541 tattcttttt ttggatgagc caacccgtgg gatagatgtt ggggcaaaat atgagatcta
    42601 cacgattatc gaagaaatgg cagcaaatgg caaagctgtc tgtcttatct cttctgaact
    42661 tcctgaaata ttagggatgt gtgatcgaat ctatacgatg aacgaaggac gtattacagg
    42721 agaagtctcg cgagaagatg cagatcagga agtcttgatg aaattaatga cgaaagaaga
    42781 ggtgggttaa gttggaacag gcaaaaaatc ttacggataa aaaagagaaa aaactaaatt
    42841 tgaaaagtag agcagctgat ctttttggtc gctacagcat ggtgatcatt ttagcgggac
    42901 tacttattgc gtttcaattg atgacggatg gtattttctg gcgtccattg aatataacta
    42961 accttgttct gcaaaatagc catatattag tactagctgc agggatgtta ttagttgtgc
    43021 tcttaggtca tgtggatttg tcggtagggt cagtgatggc gtttgttggt gcaattgcgg
    43081 gcatgatgat ggtcaatcgc caaatgaacc cgtggatgac agttatcgca tgtcttgcag
    43141 taggcgggct gatcggcgca tggcaaggtt tttgggtagc ttatgcaggg ataccggcgt
    43201 ttatcgtgac tttagcagga ttgctgatgt ttcgtggact gacacaagtt gttctaggag
    43261 gccaatctct agcacctttt ccagctgttt tccaaaaaat gtctacgggt tatttaccag
    43321 acttaagcga aggaagcagc cttcacttct tgacattgat tttaggaatg atcatcacga
    43381 tcgtgttagt tgttggtcaa tggcgaacaa gaaatcgtcg gaaaaatcat ttatttgaag
    43441 tggaatctcc aacagctttc ttggtaaagg cggtatttac agcagtcgtt gtactaggaa
    43501 taacttatat ctttgcctct tatcaagggt atccgattat tttgatcatt cttggtttga
    43561 tcgttgcagc atatgggttt ttgacaaata aaacagtagc aggtcgacag atttatgcga
    43621 cgggcggaaa tcgaaaagcg gctgaactat cagggatcaa aacaaagcga atcacgttct
    43681 gggtattcgt caacatggga gtcatggcag ctttagctgg attagtttta gctgctcgat
    43741 tgaatgcggc aacacctcaa gcaggtacat cgatggaact ggatgcaatg gcggcagtat
    43801 attttggtgg tgcttcgact tccggcggca ttggaacaat catgggtgcg attgtcggtg
    43861 gcttggtcat gggtgtattg aacaatggca tgtcgatttt aggcgtcggg gtagactggc
    43921 agcaggcaat caaaggattg atcttgcttt tagccgttgt gctagacttg tataataaga
    43981 aaaaacgaac aacgtaggag aacgccggtt attcagaaaa aagactgtga caagacattt
    44041 gtcacagtct ttttttcgct aaaagcgaac attcattgtt tcgtttgtat agactaaaaa
    44101 taatatatgt ttttttacca ttcgtataaa ttctcttttt gctcttgatt tttttgctta
    44161 attgagttaa attacatagg gcgctaaaaa aagccttgat taaattaata atgttcgtgt
    44221 tttgctggat gtgaacatca ggaggataat gtaatgaaag tatttgatta cgaagatatt
    44281 caattaattc ctaataaatg tatcgtaaat agccgttctg aatgtgatac aacggtcaca
    44341 ttaggtaaac atacattcaa aatgccagta gtgcctgcca acatgcagac gattattgat
    44401 gaaacaatcg cagaattctt agcggaaaat ggttatttct atatcatgca tcggtttgat
    44461 gaagcagctc gaattccgtt tatcaaaaaa atgaaaaagc gtggattgat tacctcgatc
    44521 agtgttggcg tcaagaaaga ggaatattcc tttatagaga agttggcaga agaatcgcta
    44581 aatcctgatt atatcactat cgacatcgcg catggacatg ctaactcagt tattgatatg
    44641 attcagcata ttaaaaaata tttgcctgaa acttttgtga ttgccggtaa tgttggtaca
    44701 ccggaagccg tccgtgaatt ggaaaatgcg ggcgcagatg caacaaaagt cgggattggt
    44761 ccaggaaaag tatgtattac aaaaatcaag acaggctttg ggactggagg ctggcaactg
    44821 gcagctctta gatggtgtgc gaaagcagct cgcaagccga tcatcgctga cggtgggatc
    44881 agaacacatg gcgatattgc aaaatctgtc cgttttggtg cgacaatggt catgattggt
    44941 tcattgtttg ccggtcacga ggaatctcct ggcgaaacaa aagtcgaaaa tggaatcgtg
    45001 tataaagaat actttggttc tgcttcagaa ttccaaaaag gcgaaaaacg aaatgttgaa
    45061 ggaaagaaaa tctggatcca acacaaagga tctttgaaag atacactagt ggaaatgcaa
    45121 caagaccttc aatcatctat ttcttatgct ggaggaagag atctggaagc cattcgaaaa
    45181 gttgactatg tcatcgtgaa aaattcgatt tttaatggag acacaatcta aaaaataaat
    45241 aaaatattag gatgttgtga aaaagttgtc ctgcattaag taataagaac agccaaacaa
    45301 aaatagttct ttatatgttt tgtttggctg gaattattat cgcagataca agcttttgaa
    45361 catcattatt cagaataagg gactgtgata agatttttgt cacagtccct tatgttttgc
    45421 aaaaaaagtt tttctttttt ctcgtaaatt cggtaaaatc aaaagaaaaa atagacaata
    45481 gaaaagagaa gcttatgtgg gaaaaagtag agaaaaaaca aggaccgatt tatcgtcaaa
    45541 ttatggaaca gattatgaga gaaatcgaaa acggaaggat caatcctgga agtcaacttg
    45601 aatcagaacg aaaactaggg caactttttg gtgtgaatcg ttccaccgtt gtccatgcat
    45661 tggaagagct tagggagctt ggggtactaa cgagcaaacg tggcagcgga tggtttgtta
    45721 atcagactga atgggggaaa ttcgctgttc caagaatcga ttggcggcaa atgatctctc
    45781 ctcgttatga gcagacggac tggtatgagc aaaaaataag agaagcaaag cagatccaaa
    45841 aaacatcgtt tcttgattta tacgctagcg agatgccaga tgagcttttg ccgaattttg
    45901 agtttccttc ttattctctt gaagaaatct tagcggaaga aaaagaaatg gatcttttag
    45961 gctatactcc gttacgtaac aaaatcaaac ggtatttgga aagtaaattt gaattggcat
    46021 tttcccccaa ccagcttctt gtgactggtg gcggtcagca agcaatcttt ttgattttac
    46081 aaacgattct ttcagctgga gaggggatag ctgtagagtc accttctttt ttttaccgat
    46141 tacctctttt caaagcttca ggaacgcgtt tatttgggat accgatggat gaagaaggga
    46201 tcgacttaga aaaactgaaa gaatcgatcc ataaaaataa attgaaagca gtgcttatca
    46261 acccgaattt tcaaaaccct acaggaaaag tgatgagtca aaaacgacga gaaaagctag
    46321 tagaattgtg tcgacagtat cggcttccaa ttattgaaga cgatgtattt acggatcttt
    46381 catttctaaa ccaagaagaa gtccttccta tacgaacgat tgatccggat aatgtgttat
    46441 acgtcggttc gttatctagg gttttaggaa aaacaacgaa aatcggttgg gtgataggac
    46501 ctgagacttt tatctcgcaa ctagctcaag cgcaagaaat gatggagttt tcgatgaata
    46561 tccttacaca aatcacagtg gctaatgtat ttaatgaagc aattgatttg aaaatgcaag
    46621 atgtaagaaa aaagttgaaa gaaaaaagcc gactgataag cgcttgggga gattcacaat
    46681 cgtttttcaa gcttcattca attttaggtg gttactatgc ctggattact tgggatggga
    46741 aaaaactaga ccccgaacta gcagaaaaag tagtagatat gggtcttgga attgcaccaa
    46801 gtctactttt tgggcaagat ataaatggaa tcagaatcaa ttacagtagg ctggatgaaa
    46861 accaaatacc gttgttttat tcaaagatga aaattctaga aaagtggtta acagaataat
    46921 ttcacaaagt aaatagatta aagaatataa cagcttggat ttaaatttca agctgtttct
    46981 tttttttcgg aaaaagaata cgatataaag aaactaagaa aaaaccatta tttgtacgtc
    47041 cgttcacgat gtgtaaataa aataaatgtt tgcaacaaaa ctgtataaca ccaacgtttt
    47101 taaatagaaa cagatggttg taagtttgat tagaaaatag aaaaaatgca gatgaaatat
    47161 aaaacgcttt ccttattgtg attttattca ctaatgctgt taatatgcgg attggcataa
    47221 tttcctttca tcgtgaaaat ttgtgctttt agcttgtatt tctcataaat ccatgataat
    47281 ttattaatga agacggttaa atagtgatac agttttattc actgtttcaa tcgcagaatg
    47341 aagagatttt cttatagatg agagaggaat gtgtgacaaa tggcaaacaa aaaagcaccg
    47401 atcgacttcc aagcactgct agatgaagtc aatgcagatt tcccagtata tcaagcactt
    47461 gatcaagatg gaaacgtagt taacaaagat ttagtacctg atttatccga tgatgaactc
    47521 gttgaattaa tgactcatat ggtatggtct cgtgtattag accaacgttc aacagcgctg
    47581 aaccgccaag gacgcctagg cttttttgca ccaactgctg gacaagaggc aagtcagctg
    47641 gctagtgctt acgcatttga taaagaagat gttcttcttc caggttatcg ggatgtacct
    47701 caactagtaa aacatggttt acctttatct caagcttttt tatggtctcg aggacatgct
    47761 gcaggtaatt cttatccgga agaattgaaa gctctaccac cacaaattat catcggtgca
    47821 caatatgtac aagcagccgg tgttgcatta ggtttgaaga aacgaaacaa gaaaaatgtc
    47881 gtcttcactt atactggtga tggcggttca tcacaaggtg atttttacga agcaatcaat
    47941 ttcgctggcg cttaccatgc aaatgccgta ttttatatcc aaaacaacgg gtatgcgatc
    48001 tctacacctc gtgaaaaaca aactgcagca aaaacacttg cacaaaaagc tgtcgcagct
    48061 gggattcctg gtattcaagt cgatggaatg gatccgcttg cagtatatac tgtatctaaa
    48121 atggcacggg aatgggcgat cagtggaaat ggaccagtcc taatcgaaac actgacttat
    48181 cgttatggac cacatacact atctggtgac gatccaaccc gctaccgtac aaaagacaca
    48241 gatgacgatt ggcagaaaaa agatccattg atccgcttcc gtaaatattt gacggaaaaa
    48301 ggactttggt ctgaagaaaa agaagaacaa gtcatcgaag caactaaaga agaaatcaaa
    48361 gcagctattg ctgaagccga caaagtacca aaacaaaagg tttcagattt cttgaaaaac
    48421 atgttcgaag tatctccaca atcaatcaaa gaacaaattg caatttatga agcgaaggag
    48481 tcgaaataat catggcacaa aaaacaatga tccaagcaat cacagatgcc ttagcacttg
    48541 aactagaaaa tgacgagaat gtcgttgttt ttggtgaaga tgttgggaaa aacggaggag
    48601 ttttccgggc aactgaagga ttgcaggaaa agttcggaga agaccgagta ttcgatactc
    48661 ctttagctga atctggaatc gcaggattga gtttcggtct tgcgttagaa ggatttcgtc
    48721 cagttcctga aatccaattc tttggtttta ttttcgaagc aatggacgaa gtagtagctc
    48781 aaatggctcg tacaagatat cgaatgagcg gcactagaaa tctacccatc acaattcgtt
    48841 cgccatttgg tggcggtgtt catacaccag aattgcattc ggataattta gaaggtttga
    48901 tcgctcagtc tcctggtatt cgagtagtga ttccttccaa cccttatgat gcaaaaggac
    48961 tattgatttc ttctattaga agtaacgatc cggttgtttt cctagaacac atgaaactat
    49021 atcgctcatt ccgagaagaa gtgccggatg aagcttacga agttcctttg gataaagcag
    49081 cagttactcg tgagggaaca gatgtatcta ttatcacata cggagcaatg gtgcgtgaag
    49141 caatcaaagc agcagacaat cttgcaaaag aaaatatatc tgttgaaatc attgatcttc
    49201 gtacagttgc gccattagat gtagaaacga tcattcaatc cgtggaaaaa actggtcgcg
    49261 tagttgttgt ccaagaagca caacgacaag cgggcgttgc cgcgcaagta gtttctgaga
    49321 tctcagaacg cgctatcctt tcattagaag ccccaatcgg acgagtttct gcacctgata
    49381 cagtcttccc atttggacaa gcagaaaatg tctggttgcc aaatgcaaaa gatatcgaag
    49441 acaaagtaaa agaaatcgcg gaattttaag aaaagaaggg acgaaacaaa gtcatggctt
    49501 atcaatttaa attgccggat atcggtgaag gaatcgcaga aggtgaaatc gtcaaatggt
    49561 tcgtcaaacc aggagatacg atcaatgaag atgatacatt actagaagtt caaaatgaca
    49621 aatcagtaga agaaatcccg tcacctgtta caggaacggt taaaaatgtg attgttccag
    49681 aaggtaccgt agcaaatgtt ggggatgtat tagtagaaat cgatgcacca ggacacgaag
    49741 acaatgaagg ggacagtggt gtagccgcag aatcccaaac tccagctaaa ccggctgctg
    49801 aacccactgt tgatacagaa tcagctggat cgtcaagcga aggagtcttc caatttaagt
    49861 taccggatat cggtgaagga atcgcagaag gtgaaatcgt caaatggttc gtcaaaccag
    49921 gagatacgat caatgaagat gatacattac tagaagttca aaatgacaaa tcagtagaag
    49981 aaattccgtc acctgtcaca gggacagtta aaaacgtgat tgttccagaa ggcactgtag
    50041 caaatgttgg ggatgtatta gtagaaatcg atgctcctgg acacaatagt gcaccgtcaa
    50101 ctagtgcgcc atcagctgaa gcaccaaaag aaaaagtgga aacatcaggt tcagcaagtg
    50161 tcgttgaagc tgccgatcca aataaacgtg tattggctat gccatctgtt cgtcaatttg
    50221 cccgtgaaaa agatgtagat atctcacaag taactgcaac tggaaaaggt ggcagagtaa
    50281 caaaagagga catcgaaaac ttcttggcag gtggtccaag cagtgcacca gcgaaatcag
    50341 aagcgccaga agcggcagca ccgaaagaag cagctcctgc agcagaaagc aaaccagctg
    50401 caccagcaaa accattcaag tccaacttag gagacttgga agaacgtgta gcaatgacgc
    50461 caacacgtaa agctatcgct aaagcaatgg tcaacagtaa gcatacagct ccacacgtga
    50521 cattgcatga tgaagtagaa gtgtctaaac tatgggataa tcggaaacga tttaaagaag
    50581 ttgctgctgc aaatggtacg aaacttactt tcttgccata tgtcgtgaaa gctttaacag
    50641 cgactgttaa gaaataccca gtattgaatg catctatcga cgatgcaaac caagaaatcg
    50701 tttataaaca ttactacaat atcggtattg caactgatac ggatcacgga ctatatgtac
    50761 caaatgtgaa agatgcagac cgcaaaggaa tgtttgctat tgcagacgaa atcaatgaga
    50821 aagcaaaatt agctcatgac ggcaaactat ctgcagaaga tatgcgcaat ggaacaatta
    50881 cgatcagcaa tatcggatct gtaggtggcg gttggtttac accagtcatc aactatccag
    50941 aagtagctat tttaggtgta ggaacgattg cacaacaacc aatcgtaaat gctgaaggcg
    51001 aaatcgttgt cggtcgtgtg atgaaactgt cattaagttt cgatcaccgt atcgtagatg
    51061 gagcaactgc gcaacaagca atgaataata tcaaacgtct tttagctgat ccagagctat
    51121 taatgatgga aggatgataa acaaatggta gttggagatt ttgctgtaga attagataca
    51181 gttgtaattg gtgcgggacc tggtggctat gtagctgcca tccgcgctgc tgaaatggga
    51241 caaaaagtag caatcattga aagagagtat attggcggtg tttgtttgaa tgtcggctgt
    51301 attccttcaa aagcactgat agctgcagga caccattatc aagagtcact agattctgaa
    51361 atgtttggag taactagtga aaacgtcaaa ctagattttg caaaaacaca agaatggaaa
    51421 gaaaacaaag ttgtccatac gttgacttct ggtgttggtt tcctattaaa aaaacataag
    51481 gttgaaacaa tcgaaggaga agcgttcttc gtagatgatc acacactacg cgtcattcat
    51541 ccagattcag ctcaaactta ttcgttcaat aacgcaatca tcgcaacagg ttcccgtcca
    51601 atcgagattc caggatttaa attcggagga cgtgtgttag attctactgg cggattagca
    51661 ttgaaagaag tacctaagaa atttgtgatc atcggtggcg gtgtgattgg tgccgaattg
    51721 ggcggagcgt acgcaaactt aggagcagaa gtaacgattc ttgaaggctc accacaaatt
    51781 ttaccaactt acgaaaaaga tctagtaaaa ttagtagaag atgatttcaa gaaaaaaggc
    51841 gtaacagtcg taacgaatgc aatggcgaaa gaagcagtcg ataacggtga cagcgtgaca
    51901 gtgaaatatg cagtggacgg gaaagaagaa tccgttactg ctgattatgt gatggtcact
    51961 gtaggtcgtc gtccgaatac agatgatatg ggcttagaac aagcgggtgt cgaagtagga
    52021 gaacgaggat tgatcacggt ggataaacaa ggacgtacga acgtacctaa catctatgcc
    52081 atcggtgata tcgttccagg ggcggcatta gcacacaaag ccagttatga agcaaaaatc
    52141 gctgctgaag caatctctgg caaaaaagta gctgtagatt ataaagcaat gccggcagta
    52201 gccttcactg atcctgaatt ggcttctgta ggtatgacga tcaaagaagc aaaagatgct
    52261 ggtatcgaag caaaagcata taaattccca ttttcaggga acggtcgggc attatcgctt
    52321 ggtaaaacag aaggattcat ccgtctagtt acgacgatcg aagataacgt gcttattggc
    52381 gcgcaaatcg gtggagtcgg cgcaagtgat atggtctctg aattagcttt agcaatcgaa
    52441 tcaggaatga atgctgaaga tatcgccttg acgattcatc ctcatccatc tcttggcgaa
    52501 atcgtcatgg atgcttcgga attagcatta ggtttaccaa tccatatttg atttgaataa
    52561 tacaggcgaa acgcaactga aaaatcagtt gcgttttgtt ttttagaatt atcaataata
    52621 ttattatgta aacttaaaaa tatgacaact tcttggttta ttaccacttc ctatgctaca
    52681 ctgaagagga atatgaaata ggagagttga agatgcaaga atatcagtat ccacttgatt
    52741 tagattggac aacagacgaa atggtcattg taatgaagat gtgggaagat ctggaacgtg
    52801 cgaatgaaaa agggataaac aatgaagctt ttttgaagac ttatcagcag tttaaaactg
    52861 ttgtcaaatc cattggtgag gaaaaacgat tgggacggga atttgaaaaa gcttccggtt
    52921 attctttata tcgtacagtg cagcaagcaa aaaaaaatcc aaacaaaact ttgagaatga
    52981 acgggtgaga taaatggaac agatgattga tcatatcaag tcctggcttg aagaagctgg
    53041 tgagaaaatc aaatcttctc aacaaacatt aacaatctcg caaaaatcaa atcgaacgga
    53101 tctagtaaca aatgtcgatc gaaacatcca gaattttcta atcgaaaaaa tccattcctt
    53161 tgatccagag gcgaaaatat tggcagaaga agaagggtgt aatcaattat cggatctcga
    53221 tggaagagtc tttatcatcg atcctattga tggcaccttg aattttgtga ttgaagggga
    53281 aaatttttgt attatgctcg ctgtttatga atccggcgtt ggaaagcttg gtttcattta
    53341 tgacgtgatg agaaatgaac ttttttgggg tggaaaagga ataggcgtgt ataaaaatga
    53401 tgagccattg cctagaccag caaataaatc cttatcggaa ggcttacttg gtgtgaatag
    53461 ttatcttttc ggtcataata aatatggaat ccgagaaatt gcggaaagca gcatggggac
    53521 acgtatgtac ggatgcgctg gtcttgaact agtggcaatc ttgaaaggaa accacattgc
    53581 ttatatttca aaccttagcc cttgggatta tgcagcggga aatgtcttat tggaagagtt
    53641 tggtatggta acaagcggtc tttttggtac tcctttgaca tttgaaaaaa gggaatacta
    53701 tttagcaggg acttcttgtg catatgcaga aatcgcagac cttttgaaaa aagaaggctg
    53761 aaaaaaaggt agaaatcatt cacaaaaatg ataaaaacga gcaaaagatt aattttctcg
    53821 aaaaaatgaa aacattttac tatatttcat gaaaaaagtc tgctataatt agcagtcgag
    53881 taaaatttgc cctgtcataa agaaccaata aaaaaggagt tagttacatt gaattataga
    53941 aacgatatcc gtaacgtcgc aattattgcc cacgttgacc acggaaaaac aacattagtt
    54001 gatgaactat taaaacaatc agatacatta gacgcacata cgcaattgca agaacgtgcg
    54061 atggactcaa atgcgctaga aaaagaacgt ggtatcacga ttttagccaa aaacacagct
    54121 gtagactata aaggtattcg cgtaaacatc atggatactc caggacacgc ggacttcggt
    54181 ggcgaagtag aacggatcat gaaaatggtt gacggcgttg tgttggtcgt agatgcgtat
    54241 gaaggaacaa tgcctcaaac acgttttgta ttgaaaaaag cactggaaca acatatcaca
    54301 ccaatcgttg tcgtaaacaa gattgacaaa ccttctgctc gtcctgagca tgtcgtagat
    54361 gaagttcttg aactattcat cgaattagga gcagatgatg atcagttaga ctttcctgtg
    54421 atttatgcat ctgcactaaa tggtacgtca agcttgtcag atgatccagc agatcaagaa
    54481 ccaaccatgg cacctatttt tgatacaatt attgaaaaaa tccctgctcc tgtcgataac
    54541 agcgacgaac cattacaatt ccaagtttca ttacttgact acaacgatta tgtaggtcgt
    54601 atcggaatcg gacgtgtatt tagaggaaca atcaaagtag gagaccaagt agcattaatt
    54661 aaattagacg gaacagttaa gaaattccgt gtaacgaaat tatttggttt ctttggacta
    54721 aaacgtctag agattcaaga agcaaaagct ggagacttga ttgctgtctc aggtatggaa
    54781 gatatctttg tcggtgaaac agttactcct gtggatcatc aagatgcgtt acctatcctt
    54841 cacattgacg aaccaacatt gcaaatgact ttcttagtta ataattcacc atttgccggt
    54901 cgagaaggta aattcgtaac cgctcgtaag attgaagaac gtttgatggc agaacttcaa
    54961 acagatgtat ccttacgagt tgaaccaaca aactctccag atgcatggac agtttctggt
    55021 cgtggagaat tgcatttgtc tatcttgatt gaaaacatgc gccgtgaggg atacgaattg
    55081 caagtatctc gtccagaagt tattgaaaaa gaaatcgacg gtgtaaaatg cgaaccgttt
    55141 gaacgcgtgc aaatcgatac accagaagaa tatatgggta gtgttatcga gtccttgagc
    55201 ttacgtaaag gtgaaatgca agatatggtc catacaggaa acggacaaat ccgtttgaca
    55261 ttcttaacac ctgcccgcgg tttgattggt tattcaactg aattcctttc aatgacacgt
    55321 ggttacggaa tcatgaacca tacttttgac caatatctgc caatgcttcc tggtcaaatt
    55381 ggcggacgtc accaaggggc gcttgtttcg atcgatacag gaaaagcaac gacttattct
    55441 atcatgagta tcgaagaacg cggtacagtc ttcgtagaac caggtacaga agtatatgaa
    55501 ggaatgatta tcggggaaaa cagccgtgat aatgatttga ctgtcaacat cacgaaagca
    55561 aaacaaatga caaacgtacg gtctgctact aaagaccaaa catctgtcat caaaaaacca
    55621 aaacaattga cgttggaaga atctttggaa ttcttgaatg atgatgaata ttgcgaagta
    55681 acaccagaaa gcattcgctt gagaaaacaa attttagaga aaaatgctcg tgaaaaagca
    55741 tctaaaaaga aaaaataatc tgtaaaagga tgaagagaga aaccagcttg ttataaaaaa
    55801 tagctggttt ctttttttgt ccaactcttt tctgtggaga agtcatcttt gtcaatggac
    55861 ataaattcag aaaatacgcc gaaagacgtt taaaagactt ttaaaaaact tatttctaga
    55921 gagtttttgt ttcataataa gataatgttt cacgaaagga gatcaacttg tccttcgaag
    55981 aaaatcgaag taaaatggca agagaaagga gcgggaagat gaacgactta ttaaaacaat
    56041 actttggcta tgatgagttt agacctggtc aaaaagaaat tatccagaaa gtcattgatc
    56101 aggaaaatgt attaggaatc atgcctaccg gaagtgggaa atcaatctgt taccagcttc
    56161 cagctttact attagatggg cttactgtgg ttgtgtcccc tttaatctct ttgatgaagg
    56221 atcaggtgga cgcggcgaat caattaggta tacccgccac ttttatcaat agctcgttag
    56281 atggttacga aacagctaga cgcttccaag aaatcgatag acagcagtac cgtttacttt
    56341 atatcgctcc tgaacgtttt atcatgccgg attttatcca agcaatgaag cgatggaacg
    56401 tccgtatgat cgctattgac gaggcacatt gtatctctca gtgggggcat gatttccgcc
    56461 caagttattt acagatggcg aaccagctag atcaactgcc aaatcgtcct gtaattgttg
    56521 ctttgacagc tacagcgact gtgcaagtag ctgctgacat aaaaagactt ttaaaaattc
    56581 cagaaaacaa ccatattcag actggttttg aacgagaaaa tctacgattt caagtaatta
    56641 aagatcaaaa aaaagaacaa tatctaatcg aatatttaaa gatcaacaaa aatcaatctg
    56701 gtatcattta tgcagcaacg agaaaagaag tagaccgtct ctatcatttg cttaaaaaat
    56761 tcgatttttc cgtgggtaga tatcatggtg gtctaaatga gaatgagcga acagaaatgc
    56821 aagaagcctt tttatacgat cgcttgcagc tgattgtagc gacaaatgca tttggtatgg
    56881 gtataaataa aagcaatgtc cgttttgtta tccattatca aattcctggc tctctagaag
    56941 catactatca ggaagctggt cgggcgggaa gagatggtct ttccagtgaa gccatcctgc
    57001 ttttctctcc tcaagatatc caagtccaaa agttttttat ccagcagtcc caaagagaag
    57061 aagggcaaaa gcaaaaagaa tatgaaaaat taaaagcgat gacagaatac gtgtatattg
    57121 agtcctgtct tcagcaatac atcctaaact attttggcga gacaagcagt ccgtgcaacc
    57181 gttgtggtaa ctgtttagat gacagagaaa tagtagaagt aacgacagaa gcacagatgg
    57241 ttctttcttg cttaaaacgt atgggtgaga actatggtaa acaaatgctg atgaaagtct
    57301 tagcaggatc gaaagaacaa aagctgcaag ctttaggttt tggacattta tctacctatg
    57361 gactgatgaa aaaccaatcg caaaaagaaa cgatgcagtt aattgaatat ttgatatcga
    57421 atggctatct tctgaccatc aatggtgagt atcctgtttt gaaagtaaca gaaagaggga
    57481 tacaagtatt gaaaggacaa gaatctgttt atcgaaagga acccaagaaa gtccagcagc
    57541 tatcggatga agaaaccgat acactatttg aagtgctgcg ggaactgcga acggatctag
    57601 catcagaagc aggcgttccc ccgtatgtcg tattttcaga ttccacattg aaagaaatga
    57661 gccgaatacg tccatcaagc agattggaga tgcttcagat aaaaggagtt ggccaaagca
    57721 agcttgataa atatggcgaa gcctttttat ctcggataaa aaatgcggac tctaacgttc
    57781 tggataagta gaaagaacga atacgtaaaa atgaataaaa acaactggtt aaatctgttt
    57841 tttttgctcc ttatgaaaat caaatatgtt ataattaagg aattagaatg ggaggtaagc
    57901 acagatggat tctttgtcga aaaaacttgc gatccaagta ttaaatgatg aagcagaacg
    57961 gatcaaacga ttaatccgaa atcaaaacaa cagtctctgt atctcacaat gcaaagcctt
    58021 cgaagaagtg gtagatacac agatgtacgg cttttcccgg caagtcggtt ttgctgtccg
    58081 tataggagta atcggaaaga acgagggcca gctgatgtta agtgagctag aacgagaatt
    58141 gaatcagtta tatagtgagg tttaccaaga aatttatgat aaaaaagaag ttggcaagga
    58201 ggaatagact tgcctaagaa agtgaaaaaa aggcatctat tagactatag catcttgatt
    58261 ccctatttaa ttttatgtgt attgggattg atcatggtat atagttctac gtcgtatcta
    58321 ttattagaaa acggacaaaa tccgtccgca tcagtcatta accagagtat tttttgggtg
    58381 cttagcttga ttgctatcgc actactttat aaaatgaaaa cagatgtttt gaaaaaccag
    58441 cgtttgatca tggcagccat cgcagttttg acgattttgc tgctgattgt tgtgtttttt
    58501 ggtaaggaga tcaatggggc gaaaggttgg ctgcaaattg caggattctc gatccaacct
    58561 gccgaatacc taaaaatcat ttctatctgg tacctgtctc tgactttatc taaaagacaa
    58621 aacagtgtac aaaaagattt tcttgggact gtgaaacgtc cacttgcaat ggttatcgga
    58681 cttactgcat tagtcgctat tcttcccgat tttggtaatg cggcagttat ctttttgatt
    58741 attttggttc tgcttttagc cagcggagtc aattatgtgt atactttgat tgtaggagta
    58801 ggaggctttt gtctaagtac ttttacgatc tggctgatca atatcacaaa cggaaagatc
    58861 ttacccggac gtttacagta tatctataat cgatttgcta tttaccaaaa tcctttctca
    58921 gacgaattaa ataaagggca tcaactagtc aacggctact atgccatgtt caacggagga
    58981 ctatttggca ggggattagg taacagtatc cagaaaaaag gatttttaca agaagcacaa
    59041 accgatttta tttatgcaat cgtagtggaa gaactgggag tgattatggg gattttgata
    59101 ttagcgttgc tattctttat gattgtgcgc attgtccttg taggtatccg ttcaaaagat
    59161 ccgttcaatt cgcttttgtg cattgggatc ggcgcaatgt ttttgatcca agtatttgtc
    59221 aatcttggag gaattactgg cgttattcct ttgacaggga tcacgttccc atttttaagt
    59281 caaggaggct ccagtttgct gatgctttcg atttgtgtcg gctttgtcct caacatcagt
    59341 gcggatgaaa aacgcaagag cttaggttta taataaaaaa agatgtagga gtggttcgat
    59401 gaaaaaagtt ttagttgcta acaggggtga aatcgctgta cgtgtatttc gtgcttgtac
    59461 agaattagga attaaaactg ttggaatcta tgcagaagaa gatgaatatt ccgtacaccg
    59521 tttcaaagcg gatgaagcat atttagtagg acaaggaaaa aaaccgattg atgcgtatct
    59581 agatattgaa gggatcattt ctatcgcgaa agaatgcgga gctgacgcta ttcatccggg
    59641 ttacggtctt ttgtcggaaa acttgaattt tgcgcaacgg tgcgaagaag aaggcatcat
    59701 ttttgttgga cccaaactgc atcatttaga tatttttgga gataaaatca aagcaaaagc
    59761 tgcagctatc gaagcgggga ttgcttcgat tccaggcaca gatggaccaa tcgcttctat
    59821 agacgatgcc ttggagtttg cgaaacaata cggttatccg attatgatca aggcagctct
    59881 aggcggcggc ggacgcggca tgcgtgtagc tcacgatgaa aagagtgcaa gagagggcta
    59941 cgaacgtgcg aaaagcgaag caaaagcagc ctttggtagt gatgaagtgt atgttgaaaa
    60001 atacatcgct aatcctaagc atatcgaagt acagattctc ggggatacgc atggaaacgt
    60061 catccatctt tttgaaagag attgttctgt tcagcgccgt caccaaaaag tagtagaagt
    60121 agcgccttgt gtctctatga atgaacagca acgtcaaaaa atctgccagg cagctgtcca
    60181 attgatgaaa tatgtcgggt atgtcaacgc aggtacagta gaatttcttg ttgaaggaga
    60241 tgatttttac tttatcgaag tcaaccctcg cgtccaagtg gaacatacga tcacagaaat
    60301 gattacagat attgatatcg taacaacgca actgttgatt gctcaaggtt tggatctgca
    60361 taaagaaatc ggattgcctc agcaagaagg aattaaatta aatggttcag ccattcaatg
    60421 ccgaatcact acagaagacc cgttgaataa ctttttacca gacacaggga aaatcgatac
    60481 gtatcgttca cctggtggtt ttggcgtacg tctggatgtc ggaaatgctt acgcgggcta
    60541 tgtagtgaca ccgtattttg attcattgct agtaaaagta tgtacgcatg gtgctacctt
    60601 tgaaacagcc attcagaaaa tggaacgttg cttgaaagaa tttagaatcc gtggagtgaa
    60661 aacaaacatt ccatttatgc tcaatgtcat cacacatcct gaattccaat caggaaatgc
    60721 aaaaacaacc ttcattgata gtacagcaac attatttgaa tttcctcgac tacgagaccg
    60781 cggaaacaaa acgatgaaat atatcggtga aattactgtg aatggttttc caggtatcga
    60841 aagtggcgaa aaaccatttt atgaagaacc acgtatgcct aaagatctaa tcacaagaac
    60901 tgattacgta acggctaaga atgtattaga tgcgaagggt gccgatgctt tggtcgaatg
    60961 gatcaaagga caggaaaatt tgttgttgac ggatacgaca tttagagacg cccatcaaag
    61021 tctgctagca actcgtgttc ggacaaagga tttcaagcag attgcacgtt tgactggaga
    61081 aggtcttcct gaactctttt caagtgagat gtggggggga gcaacatttg atgtggctta
    61141 ccgctttttg aatgaagatc cttggcaacg gttgagaaaa attcgttctt taatgcctaa
    61201 tacgttgctg caaatgcttt tcagaggttc aaatgccgtt gggtattcta attatcctga
    61261 caatgtattg gtggaatttg tcaaagaagc agcagcgcaa ggaatcgatg tatttcgaat
    61321 ctttgatagt ttgaactgga ctccacaaat ggaaaaaagt atccaagctg tacgggatac
    61381 tggtaaaatc gcagaggcag ctatttgcta tactggcgat atcaacgatc caagtcgggc
    61441 aaaatacaat gttcagtatt ataaagacat ggcaaaggaa ctagaacaac tgggtgcgca
    61501 tatcattgca atcaaagata tggctgggct gcttaaaccg caggcagctt atcgtttgat
    61561 tagtgaattg aaagaaacga cagatctgcc aatccatctg catactcatg ataccagcgg
    61621 gaatggaatc atcacttatt ctgctgcttc aaaagcagga gtagacatcg ttgacgtggc
    61681 aatgagtgca atgagtggga atactagtca gcctagcatg agcagcttgt attatgcact
    61741 ggtcaatggt ccacggctgc cagaaatcac gattgaaaat gcacagaaat taaaccatta
    61801 ttgggaagat gtgcgcatgt attacaaacc gtttgaaaac ggcttgaatg ctcctgaaac
    61861 agaagtatat atgcatgaaa tgccaggagg acagtattcg aatctacagc aacaggcaaa
    61921 agctgtcgga ttaggtcatc gctgggatga aatcaaacaa atgtatcata ctgtcaactt
    61981 gatgtttggc gatatcgtta aagtaactcc atcatctaaa gtagttggag atatggcact
    62041 atttatggtc caaaatgatc ttacagaaga agatatttat gaaaaaggcg aaacactaag
    62101 tttccctgaa tctgttgtga ctttcttcca aggagaacta gggcaacccg ttggcggatt
    62161 tcctaaaaaa ttgcaaaaga ttattcttaa aggcagacct gcattgaatg aacgtccagg
    62221 tcttttggct gaatcagtcg acttcaacga agtaaaaaaa gaattggcag aaaaaattgg
    62281 ctatgaacca aaacaagacg aagtattaag ctatttgatg tatccgcaag tctttttgga
    62341 ctatcaaaaa gcatataatc aatttggcga tgttacatta ctggacacac caacattttt
    62401 ccaaggtata cgtttgggtg aaacaatcaa tgtacaaatc gaacgcggaa aaacgcttat
    62461 cattcgttta gatgaaatcg gagaacccga tatcgaaggg aatcgcgtac tgttcttcaa
    62521 tttgaatgga caaagacgtg aaatcactat caaagataat tcgatcatca gtacggtcca
    62581 aacccgattg aaagctgaac cgacaaaccg agaacaaatt ggagcaacga tgtctggttc
    62641 tgttttggat gtattagtga aaaaaggcga taacgttaaa aaaggtgata cgttgatgat
    62701 tactgaagcg atgaaaatgg aaacggccat cgaagcacgt tttgacggag aagtcgcaca
    62761 tgtatatgtg tcttcaggag acactatttc gtctggtgac cttttgattg aagtgactga
    62821 gaaataataa gcgaaaggtg gggaaacatg aaaagactgg gtttgttttg gctgattctg
    62881 ctattagtta ttgtggcatt ctacatggaa ccagtcttgt ttccgccaaa acttgaagtg
    62941 aaggaaaact ctgatcaaca aatatcaaac caaatgacaa cgtggaaata ccaggagctt
    63001 aaagcaactg gtttttctac ttatatcgga aaaccgatcg atcagctgga aaaggatttt
    63061 ggccaagcct atgatcgatt aactagcgga tttggttttg aaacaagata ttatgaaaat
    63121 aaagctagtc atctcacatt tgaagcgaac atccaagaca gcagagtcac aactgttaaa
    63181 gtactgcaaa cagggcctgc tgatatcgcg ccgttttatt tagggatgac gatgcaagat
    63241 cttacgaaaa tcactactat ctatactaat ttcacttttg agtaccatga tacagaagtt
    63301 ggaatcgaac taatggaaga ggatatgaat tatcgtccgc taattgcttt tgataacgac
    63361 acttttgcta ttttattttt tgatcaaaca acaaacggcc tattttcagt tgtgtattta
    63421 gataaagata gtcttttgaa actattgcca tatcaagtgt ttggtgaagg ccttccacat
    63481 tatcaagcag ataaatcggc tgattgggaa tcaatcaatc gcgttaaaga acgaaaaagt
    63541 acgactttat taaatatgct tagaagagaa gacgaacttc ccatatacaa tcaaacactt
    63601 gcctttcaaa accacagtca gctgctacta catgattttt tgacaaggcc tgaggaaata
    63661 ttgtcagagg aacgtatggc tgattggcaa aaatcgctag gcagtgctca aacgacagtg
    63721 aaattctcac tgacgaatga tgaattggag aagttaatca gtaaacaaaa attagaaaat
    63781 actagtgggc tatttattca tccggcgatt gacgctactt tttcgttttt atattggtac
    63841 agcgatcctt acaaccatga ccgtttcatg gacccgggaa cggattctgt aggcattgct
    63901 ttttctaaag aaaatatgtt agtcttattg caagaagaaa acggagaaag tagtgatagc
    63961 agtgatcgtt aacgaagagc tgtttcaact ggaggatcaa tgcctccaag tcgctgaaat
    64021 gataaaaaag agtaaaacga tggcttctta tcttgaacat aaacagtcaa tggaagaatc
    64081 tgcaaaagtt tgccagttga aagaagcgtt cttgtctaag aaagaagcat ttgaaaatgt
    64141 agcggcatat ggtgagtatg cacctgatta tcgtgaaaaa aaacgagctg ttcgaaaagc
    64201 aaaaagacag cttgatatgg atgagcatgt tgcagctttc cgatatagtg aaacacagct
    64261 gcaaagtctg ttggatcaaa ttgtccaagt acttgccgaa gaagtttcta atgaaatcaa
    64321 agtagatgca ggtagcccgt tttttgaaac gaaaaaacac tttggatgcg gaggtaattg
    64381 tcatgcaatt agacaatagt gaatttgaaa taacaaaacg acgcgggctg atcgtatggg
    64441 tctacagctt aaaacaatta aagaatctga aaaagttcgg cttgttgcat tatgtctcaa
    64501 gaagaatgaa atatgtcgtg ctttacttaa atgaagagac attggaagaa acagaagaac
    64561 gaatccgtaa attacatttt gttcgaaacg tagaacgttc ttaccgaccg gatatcgaaa
    64621 tgaattttgc cgaaaaaata ggaaacaaaa cagtattaaa agacgaaggt ttcgaaattg
    64681 aagagctaaa tacagaaatc cgattagctg aacatcttta aaaatctctt atgagcagtt
    64741 tttaaataaa aaataacttg gataacgatc gatttttgat cattgtccaa gtattttttt
    64801 ggtatactag taccagtacc atttctaagg agtgagtaga tgcggataat atcaggaaat
    64861 tatggcgggc gccgtttaaa aagtttagca ggggcgaaca ccagaccaac tacagataaa
    64921 gtaaaagaat ccatattcaa tatgattgga ccatattttg atggagagac agtattggat
    64981 ctgtttgcag gaagcggtgg tttagctatc gaagcagtat ctagaggctg cagtcatgcg
    65041 gtatgcgtag acaaaaatta ccaagcgcta aaaatcatca aagaaaacat cgaaattacg
    65101 aaagaaccag agaaatttac cacaataaaa cttgatgcgg acaaagcaat cgaacggcta
    65161 gcagcggaga aacaacaatt cgactacttg tttcttgatc cgccatatgc aaaacaaaaa
    65221 ataatcgatc aaatccataa aatggaagaa ctgggacttt tttctaaaac cgcagttatc
    65281 gtatgcgaaa cggataaaac agtcgaattg ccagaaactg tggctggatt ccatcaaata
    65341 agaaaacaga tttatggcat aacggccgta actatctata gaaaagaggg aaatcaatga
    65401 aacgtgcatt attcccagga agttttgatc cttttacaaa aggtcactta gatacggtgg
    65461 aacgtgcggc taaattattc gatgaggtag taattggtgt ttttatcaat acttcaaaga
    65521 aaagtttgtt tcccccagaa gaaagaatga cattgatcac aaaagctgtt tctcatcttc
    65581 ctaatgtaaa agtcatgcat caagaaaatc aattgacggt agaaacagca aaagagattg
    65641 gtgcagatgc gttgatcaga ggcatacgaa gtatcaaaga ctttgaatat gaacgagaga
    65701 ttgctcagat gaatcatcat ctatatgatg aattggaaac tgtcttctta ttagcaaaac
    65761 cagaatatag tcacgtgagt tccagtattt taaaagaagt actgcacttt ggcggagatg
    65821 tgagcagcta cttaccgcca gtgatcaacg aagctttagc tggaaagcgt gaatcaaatg
    65881 aaagctaaac aatggttgaa aacagtactg attatcatat ttggaatgtt gatcattgga
    65941 tttgtagtag ttcccttgcc gtattatgta gaatcaccgg gagccacgat cgatctgaaa
    66001 gaactaatta cggtcaatga taaggaagat aaaggttctg ggtctttctc tcttacctct
    66061 gttggtatca ggcaagctac aggtttctta gctttaaaag ctaaaatgac cccttttgaa
    66121 gaattgatca gtgaagaaga attgactggt ggggcgacaa atgaagaata taatcaaatg
    66181 caacagtatt atatggattc ttcccaaaat gcagcaatcg aacaagcgtt aaaacttgca
    66241 gacaagcctt ataaaatgga attcaaaggc gtatatgtca tgagtgtaga gaaaacatcg
    66301 aatttctttg gcaagctttc agttggagat acagtaacaa aagtagacgg gaaaagcttt
    66361 caaagtacag aagcatttat ggattatgta aaaagccaaa aagttggaca aacaatcgaa
    66421 atcagttatt tgcataatgg tgaagaaaaa acagcttctg gtgatctgat cgagttacca
    66481 acagataaaa aggcaggaat aggtatcacg cttactgacc ataccgagat cgaatcagat
    66541 acagatgttc gtattaattc tggctctatc ggtgggccat cagcgggatt gatgttcact
    66601 ttagaaatct atgaacaagt cactggtaaa aacttgcgac atggtaagca aattgcagga
    66661 actggaacga tcaacagtga aggagaagtt ggccgaatcg gaggaatcga taaaaaagtg
    66721 gcaagtgcgg ataaagctgg cgtagagatt ttctttgcac cagatgatac gatttcagac
    66781 gatgttaaga aagaatatcc tgaaataaaa accaactacg aagaagcaaa agctgccgct
    66841 aaaaaattga attcctcaat gaaaatcgta ccagtaaaaa cagtacaaga agcgcttgac
    66901 tatctagaga aaatgaaatg aaactaaata attaaagagg ggttgtgaca cgagttctgt
    66961 cacaacccct ctttttcgtc aaaaatcttc acgataaaaa taaaaacaaa agaaaaaatt
    67021 gacgtaatta gatgttgaag ggacgatttt gccagagctc actgtttctt caattttaaa
    67081 tgaaaggagt aatgaatgat ttttactggt cattctttga aaaaagaaca atgatagaaa
    67141 aaatcagcaa gaaaatacta gcttcaccca aattgatcat ttggatgtct atgatagttc
    67201 ttatcttttg tatcgcaagt ggttttttat tatcaaattt gctattttat agagaaacag
    67261 aagagactgc tgcaaaagaa atactttctt cgtttagttc cagtggagaa gatccatcag
    67321 ctgaaggaaa cgatgtagaa gaggatgctc gtccaaaggt catgtacacc gatatcaaag
    67381 gaagtgtgaa ggagcctggt atctattcct tttcttcaga agaacgggtc tatgatgtac
    67441 taaagcgtgc aggtggatta ttagaagaag cggatagcga tcgaatcaac ttttctgcga
    67501 agatagaaga tcagcaagtc ctatatatcc ctgctgtagg tgaagagctg ccagaacatc
    67561 ttgatcagtt ggcatcctcg gaaggaaaac agcctacagc agattccgag cctcccaaaa
    67621 taaatatcaa tacagccact ccatcagaac ttcagcagat tcctgggatc ggatcagtca
    67681 aagcgcaaga aatcattcga tttagagaag aaaacggatc cttccagaaa gtggaagacc
    67741 tgcaggagat ttctggtatc ggtgaaaaga cagttgaaaa actaaaaaac ttcgttacaa
    67801 ttaaatagaa tgattcaaaa tcgactggca cgagcaataa atggacgcta atggatacta
    67861 agaataatcc aattctaaga gcttggcagg acgaggaggt agtatttttg gataggaaat
    67921 agcttgattg ttcgcaaaga aacattaaaa aaaccaatgc tgaatgtcta tctccaaaat
    67981 aaaatatctg ggattcatat tatgaatacg gctgtcagcg gcaataacag tcaagctcta
    68041 cgagaacgct ttgccaaaga tgtacttagc tatacagcag ataaagtatt tattctcatt
    68101 gggacgaatg atctagcaga gcataaacaa ctttctaaag aaacgtacca aaaaatctgc
    68161 agtggatgat agttcagctg aaaaaagact atagttctga tcatgctttc tttatcacgc
    68221 caccagcagt cgatgagaca aaacaaaaat atcggaataa caagttgatc aaagaatata
    68281 tctctgttct cacagaagta gcaaaacgcc atgattgtga agtatcgatt tttatacgga
    68341 atttcttgaa caaatggatt cgaagaattt ttataatggg ctaatggatg acggacttca
    68401 tttcggcagt gaagggtagg atcttttgtc agacaggatg attcgttata tttaaagaat
    68461 attttgttta agcaatgagt actttttaat caacttgaaa aaacggagga atataaaaca
    68521 tgttagaaaa atcttcaaaa gcatgcgata cgaataaaga aaaagttact gataagtcac
    68581 ataatgaacg cattccttgg gaccagtact ttatggctca ggcagtactg ttatctttac
    68641 gtagtacgtg tacacgttta gaagtaggag cgacactcgt tcgagaaaaa aggatcattg
    68701 ctggcggcta taatggagca gtcagtggag atgtccactg catagatgaa gggtgttacg
    68761 tcgtggatgg acactgcttg cgaacgatcc atgcagagat gaatgcactc ctacagtgtg
    68821 caaaacttgg tatcccgacg gaaggttcag aaatctatgt cacacatttt ccatgtttgg
    68881 cctgtacaaa ggctttgttg caagctggtg tgaaagggat caattatctc catgactatc
    68941 gaaatgatcc ttatgcccaa gcgttgatcg aacaattagg tgtatctgta caccaagttg
    69001 ctttagactc acagcatttt tcaaaacttc aaaatgaatt aacaggaaaa catgtctagc
    69061 gttttagaaa ggcatgttcg aaatcggttg atttttccgg caattacgtc gatgattatc
    69121 tgtttgttgt attacactga caattggggt tatcgtttgc ttcttgccgg aattttttta
    69181 tggttttcgc taaaagaagg acgttttatc gcgtacttgt gtattttgtg tagcataacg
    69241 gtatccattt cttataggct acaagagtat tcatttttag aggaaaaaac aacatctgcg
    69301 caaattactg tatttacaga tacgattcgt gcaaatggta attttttgac aatggaagga
    69361 aggttatcag agaataaaca aaagctccag ctttcttatc aagtaaacac agaagcagaa
    69421 ttgaaaacat ggcttaactt ccgaggaaag ggaatagaat tgactgctga aggcgagttc
    69481 atctctccag caaaaaagcg gaatctttat aattttgacc aagaagctta cttcaggaac
    69541 catagaattt ctggacggtt ccaaattcaa aagattgaac agatcaccat gcaaaatcac
    69601 tggcgaaact ggcttcagag aaaaagagga tcttttatct cctttgtcca atctgaattt
    69661 ccgcaaaaaa caagtgtata tattaattct ttattatggg gctataaaga tgcaggattc
    69721 agagaaccag aagaaatttt tagggagagt ggtttgctgc atctgttcag tctatcgggt
    69781 ctacacatcc aattttattt agggtggctg tattatttat ttcgacgtat cggatggcca
    69841 ctacacgtca gtattattcc gttgagtcta ctaactatct gttatgtttc tattgcagga
    69901 agttctattt ctgttttgcg tgctagtttg ctgtttttat tgcgattgct agtcaaattg
    69961 ctgaataagc attattccac catggactta tttgcagttg tcttttggct tcttttacta
    70021 tttgatcctc gatttctttt tcatgcgggt ggacagctta gttttttcat gagttttttg
    70081 tttatcctga tttcttttga tggagattgg ttgaaaaaaa cggcctctca cgtcctgttt
    70141 actatgatca cgatgccgtt gattgtatgg catttttatg agtggcctat tttaggtagt
    70201 ctttttacat tactgattgc accggtattc aagtttcttt ttcttcctgg tgtgttattt
    70261 ctcacactct tcaatcgctg gctgccggaa actttacttt tgctgataga agagggatta
    70321 atcttatttg agcgaataat cgatttttct tcagggtcgt ctctcattat aggacgtttg
    70381 ccccttgtgc tatctgggat gtatattttc ggattactga tttgtatgga taggttgaaa
    70441 gaacaacctg tgaaacactt gttttatgtt ggtctttttt cttttatttt tttgactttg
    70501 ccatttttac gcttttcttc aacgatcgct tttatcgatg tcggtcaagg agacagtatc
    70561 tttttgcaaa gtccttttcg aaaagaaacg attttgatcg atactggagg tcagttacac
    70621 tttgaaagag aaaagtgggc gcaaggtatg atacgtcctc ctgctgaaag aaatatcgtt
    70681 ccatatctaa aaggtcaggg gataagtgta atcgataaac tcatcttgac acacgacgat
    70741 acggatcatg tgggagaatt acccacgtta tctcagcatt ttactatcaa aaaaatttat
    70801 atcggttggg gcgctggacg aaatgagcca ttacaaaggc atttatctga agctcaaaaa
    70861 aatggtactg agatcattga aatcaaacat ggggacagga tcagcggtta ttatgatctt
    70921 tatgtattga ctccttttga aaagggagag ggaagaaatg aagattcgat tggtctatgg
    70981 atggaatata acaataggcg ctttttattt ttaggagatt tgaatcaagc aatggaaaaa
    71041 caattgcttt tgatttatcc taatctgaaa gccgatgtag tcaaattagg tcatcacggt
    71101 agccgtacgt caagtaacac agattttctt tcccatattg aagcaaaaca tgggattata
    71161 tcctgcggcg taaataatcg ttatggtcac ccgcacagcg aagtattgga gacactagaa
    71221 gaaaatcaga taaaaacatg gcgaacggat caacaaggaa tgatcaccta tcgctggcat
    71281 ccagtttttc atcctcatgg gctagtggaa acaatgatag actagtaatt ctcatctgct
    71341 catggtagga taatttttga aagtggtgag taatttgagt atacaagcag agttacaaaa
    71401 aatacgtaaa gaaaaacttg catcttgtta cttgatttta ggaacagaaa agtttctcca
    71461 agatcaagtt cggacagaaa ttgtcaaaaa aatccaatta gagggcgaag atgacttaaa
    71521 ttttttaagt tttgacatgg aaaatgcaag tttagatgag gtagtagctg aagcagagac
    71581 gttgccgttt ttcggagatc aacggttagt attcgtagag aatccttatt ttttaacagg
    71641 tgaaaaagta aacaatggaa tcgaacaaaa tactgattta ttgacggctt atttgaaaga
    71701 accattagaa tcgactgtat tagtattctt tgcgccatat gaaaagttgg atgaacggaa
    71761 aaaagtaacg aaacaattga aaaaaacagc aattatcatc aatgtccaac agttgaatga
    71821 aaaagaagta cgtcaatatc tgatgaatac cttagagaat tcctcaatca aaatggaacg
    71881 aagtgcaatt gatttattct tgaggttgac cgacttagat ttatccaaaa tgatgagaga
    71941 gttacagaaa ttggtccttt atggacaaaa tcaacagcaa atcacggtaa gagaagtaga
    72001 agaattagtt cctaaaacgt tagaacataa tatttttgac atgacccaat atattttaaa
    72061 agggaagacg gaacaagctt tgcgtttata cgaagattta gttatgcaag gggaagaaac
    72121 aatcaaaatt aatgcgattt tgctttctca attacgttta tttttacaga caaaatttct
    72181 tgtaaagatt ggttatcagc aggcaaatat tgctgaaaca ttgaaaatcc atccatatcg
    72241 tgtaaaactt gccatgcagg aagtaaaaaa attcgatgaa catttgctgg tacagttgtt
    72301 tgatcaatta gttgagatgg attatcaaat caaaacggga caaatcgaaa aagaattaag
    72361 ttttcaacta ttcgttttgc gcacagggca gttgttaaga aatcgataag aagaaagacg
    72421 agggcaaagc atgcgggaag gtcgtttgtt tttgtccttt ttttgacaaa taaaaaaatc
    72481 ggccgaagcc gattctttta tttagcgatt tttttgctta aacgagattt gtcgcggtta
    72541 gctttgtttt tgtggattag accttttgat tcagccatat caatagcttt aacagcttct
    72601 ttatataacg catctacgtt gtcagcacct gaagctacag catcttcaaa ttttttgatt
    72661 gcagtacgca tagcgcttgt ttgagatgaa tttttcacat tagcgttttc gcttgtacga
    72721 acacgtttga ttgcagattc aatgtttggc attagtttca cctccgataa cttaagttct
    72781 tacgcgtgtt accgcataat tcaacattca ttattatacc taaaggcagc gtgctttgca
    72841 atatgaacat gacaagtttt tttccttaac agtcttttat taggattttt tttcagtaaa
    72901 aacggatagt acaagctgaa aaaaatcaaa aagacgctat tgtgggaggc aatagcgtca
    72961 aaccaaacta gatttttatt tatctattta aacaaagttt gaatgggaat gtttggtact
    73021 aagttattat ataacaaaga gaaccgtttg caaagttttt tttgaaaagg gatacgattc
    73081 cctgttgttc tttgtttttc tattaagaaa ctgcagttat tttataacca gtatcctttg
    73141 atcagtaaag gctattctgg tgtaccgatt attttaatgt attcatcgat attttttatc
    73201 aacatttttt ttatgaccaa ctggttccga cggttccatt tgccacttac ttccagcaca
    73261 tatttattta ggggcaaacg taagatatat tttgccaaat agcgatcttt aagcaggcca
    73321 ttgatcacac ggtcctcaag ctgcaagttg atcccagtca gcatcattgg ctgttcttgt
    73381 aaaacgtacg tatcaattaa aagaccttca aaagtttcca caagcgtctt cctttctttt
    73441 caaagtaacc tgaaaacagg cactaattct tccattttga tattcgttga agtggatgcg
    73501 atcattcttt cttttgtcca ctgtaatcta gtataacaaa aaagagcaga agagccaaaa
    73561 agaaaaaaga gcatcgaatt ctataggaag tctcattggc acggcctaaa ttattacaaa
    73621 acattgatta aaaaaagccc ctgcatcgca ggagcatccc ctatatcgtt ttaataggtc
    73681 aatcaaaaag cagtatcaaa cacattttgt atcaaatcag gaaataatag ataagctaag
    73741 cccagaatca aaagtaccgc tagaagaaac cagccgattg ttcctatgaa atactttaat
    73801 ttttcgaaca ttgtatcacc tcgttgtcag tatatcacgg atcagtaaat tgggggtgtt
    73861 aagaatctcc taaaatagac aaaatttgtt ctattttcct aaagcaaata cttgtaagat
    73921 acagtaagtg ataccagaag caatgaccgg tccggcggcg attcctctta agaagacaac
    73981 acctaaaatc gtgccgaaaa caagtgcaac agtgatttgt ggatcagaag caatcaatcc
    74041 tacacccttg gaagataaga cagccactag cccaccgcaa aaaacagcta cccagccaat
    74101 aggggattta aaggcttcta atagatcacg tagaccgatt tctcctgttg cgatagggat
    74161 tagaatagcc gctgtgatga tcgtcacccc aatattgatt cctttgcctt gaagccaagg
    74221 gaaaaaggaa cgggaggctg gtatgagctt cataatcaaa acaaaagctg ccgctatcat
    74281 cagactttga tttttagcta aaattgctac aagtaaaata attcctaaaa aaagccaact
    74341 ttccatgaaa tatattcctc actttcttcg aaacaatcat aacatgtttt tagaaagtta
    74401 caaaagtagg aattcatcct tcaagaccta ttcataagct gtttttttca taaaataaac
    74461 aaaatcataa aaaaacactt tgcttatttg acagaagaca gccgcgcttt gtatagtgga
    74521 caagacttac atttttttga agaatcagaa agatcaggta aaggtcttag taaacgcaag
    74581 ccattcagaa taaccaatat tgtgcttcct tcgtgtccta caactccaag aggtagattt
    74641 aaaaattgca ggaagttaga agcaatcaac aagagaataa caagaatcga aaaaataata
    74701 ttttgtttga tgatccgccg catttttaaa gatagccgat gactgtagac aaatttattt
    74761 aagtcgcttt tcattaagac gatgtctgcg atatccatag cggcatctgt cccttgcccc
    74821 atagcgacac ctaaagaagc ggctgctaaa gcaggcgcat cattgactcc atcaccaacc
    74881 atcgcattta cttggtaaag ttcattctct tttttgacta gttgtgtttt ttcttccgga
    74941 gtacagcttg catagtaatg ttcaatccct acttgttcag caatggcctt tgctgtgcca
    75001 gaatggtcgc cggttatcat acttgtatgg atgttttggg atttaaagta ggaaattact
    75061 tgttgggtat ttgcttttgg aatatctagc agacctaaaa cagcaattaa ctgctgatct
    75121 ttagaaagat aaatcacggt tttgccttga ttttccaaac gttcgatcat ttccgcaatc
    75181 tctttgctga ttatcatcgt ctcaggatca tatgcgtgct tgccaacttt ccattgcgca
    75241 cctaaatagg tagtttgtaa tccaaaacca gtaatttctt ctattggtaa gtattggatt
    75301 tcttcaggta ttgtgcaatc gaaatattgc aaaatcgcat gagcaagtgg atgcgtcgtt
    75361 ttttgttcca tagcaacaag aagttgttgt gccaaatcct tttcatcaaa gaaaaagctg
    75421 tcagttacaa ctggttttcc tttagtgagc gttcctgttt tatcaaaggc aatcgctttt
    75481 aattcagaaa gcttttctaa atggataccg cctttgatta aaatcccatt ttttgcagaa
    75541 tttgaaagag cagctagaga agctggtgta gcagatgcga caagcgcaca aggagaagcc
    75601 acaactaata ataccattcc tcgataaaag ctttcatccc aactccaacc taggaaaaag
    75661 tgggggagaa ggatcattag aggaacagca agcaaaatca attttacata attattttcg
    75721 attcgttcga taaatgaagc tgtttgagtt ggcgtattct gcgcttcttc tactaattgg
    75781 ataatcttag caaagacagt ttcggaactc gtttttgtta ctcgaatcgt gatgggattt
    75841 ccttgattca gcgttcctgc atacatctca tcgcctaaat atttttctac aggcacagat
    75901 tcaccgctta atgcagactc atcgatcgtt gcctgtgtgc tttctaaaaa gccatctatc
    75961 ggaacagctg ctcctttagc gacaaatacc aagttatcca cctttagttg agaaacatct
    76021 acttcttgca tttgtccatt ttcttttaat aattgggctt tttgtggttg aagattcatc
    76081 aaagcagtaa tttctttttg acttttatta gttgtgtatt cctctaatgc accgcttaaa
    76141 caaaagatga atgtcaacat tgctccttca aagtaattac cgatcaaaca ggcaccaata
    76201 gcagctaaag ccatcaataa atccacattc agtgtccgat cattccacaa ttctttcaag
    76261 ccttccgatg tttgtttata tccaccgatg ataatggcac tgatataaaa aagtggggtg
    76321 atggtaagag agaatcggtc gaagataaaa ccgaagatca taaataaaag gcagccgatt
    76381 gttgctgcaa aagctttttt ctgattagtc atcgttatta gacctccttg tcaaaacaag
    76441 gatagcataa taaaaatgta attgataata ataacagcta aatgagaacg ataatggtta
    76501 tcatttggtg attaagcttg atgttgtttt tattatttat aatgattatt attaaagaaa
    76561 ataaaaaacc agaacatttc tcaaataaga gaagtgttct ggttttttct acattgcaat
    76621 aaaccaataa ccaatcaaaa tgataccaag gataatacga taccagccaa aagcggtaaa
    76681 gtcgtttttc ttcaaatagc ttagtaagaa tttgatagct aagacggaaa ccacaaaaga
    76741 tacgaatgtt cctacaagaa gaacaacagt ttcttccatt ttgaatgaat tcccttcagc
    76801 taaatatttg acgatcttca agccgcttgc gccaaacatg acaggaatcc ctaagaaaaa
    76861 tgaaaactct gtagcgacaa aacgcgaagc accaatcaag atagatccta ggatcgttgc
    76921 gccagaacgg gatgttccgg gaacaagtgc caagacttgg aataacccaa caagaagagc
    76981 tgcttggtac gtaaagtcat tcagagaagt ccaacgaggt gttttgtttt tgttgcgttt
    77041 ttcgacaata ataaaggcaa taccgtaaac gatcaaaaca atggcaactg gtaagaactt
    77101 gtggaaattt gcttctaacc aatcatccaa aggcaaacca atgatgatgg ctggtatgca
    77161 agcaacgatc actttaaacc aaagtgtcca cgtatctttt ttttctaccg ttgatttact
    77221 tggagaaaat gggtttaact tgtgaaaata aagaacgacg acagccaaga tcgcccctaa
    77281 ttggataacg acattgaaca tcgacataaa ttgtgaactg gcatccagtt tgatgaattc
    77341 atctgctagg atcaaatgac cagtactact gatgggcagc cattccgtta ttccttcaat
    77401 aataccgaga atcactgctt tgataatatt tgcaaaaaac ataaattcct tccttcccaa
    77461 ttaagataca tcttatagta taccgttatt ttttgagaaa cccaatctgt tttttaatat
    77521 taagaaaaac ttgagaaata aagcagataa gtacacgaag agaaaaaaga agacaaagcc
    77581 ctcgttcaga ctttgccttt tatgattctc tggtaaaaat taaaatatcc ccattataga
    77641 aacgagtcgc gactatgtgt tttcccgtta tcgatataag acgtatcatt gatgccgtgg
    77701 atagaaaatc gaccgtttgc ataagtgacc tttgttacgc tgccatttaa aagcccagca
    77761 cgaacaggct gtttaggatc gatcagatta agcaaaaagg cgatcgtcaa accatgagat
    77821 acgactaaaa catttccacc accatttttt tcgcagtgat gggcgatatc ttcaaatcct
    77881 gtccatacac ggtctcgcaa tgtttcataa gtttccgccc acccagcggt atctaagttg
    77941 tatacagcat tagcaatttg ttcaaaagaa acttctgtgg taaacatttc atcatatgtt
    78001 ttaaaattca atacacgagg gatcacaccc cacatttcca tgtcatatcc accttcaaga
    78061 gacccgaaac accattctct aatccttttt tcaataaaat aaggaatttc ttttccctta
    78121 gaatgttctc ctagaataat acgggcagtt tcgattgcac gaccactgtc actagaataa
    78181 gcaaatgaaa agtcgacatt ttttaacccg cgacctaaat aatgaatgcc ttcttgtcct
    78241 tgctttgtga ggggtgtatc acaccagcct tgtactcgtt caattgtatt gaacatcgtt
    78301 tttccgtgtc gtacgatata aagtgtagtt tctgtcattt tattctcctc ctaagtcgtt
    78361 ttaacgattg aaaaaaggat ttgtcctttt ttcctgttca atcgtcgttg catcgccatg
    78421 tcctggatag actgcaaatt catcaggtaa tacaaacagc tgggtccgaa tactatgaag
    78481 taattgttct aaattccctg tatacagatc ggttctaccg atacttcctc taaacagggc
    78541 gtcgccagaa acgacaaaat catcaaaaat aaagctgacg cttcctatag aatgccctgg
    78601 ggtagggacg acgcgaaaag acatgtttcc taaagtataa gttttcattt caaattcata
    78661 ttctgcaggc gagacaataa catcagcaat atcatcatgt ctaccgagtc cagaaagatt
    78721 caaaatcgga tcacctagcc atttttgctc tagtgggctg acataaactg ggatttgata
    78781 atacgcgcgt aaaggttcca ctgcaccgat atgatcgtag tgagtatggg taagcaaaat
    78841 cgctaccggc tgttggtttg tttttttgat ttcttgtttg attcgttcca catcttctcc
    78901 agggtcaatg atcaaaagtg cttcttcgtt gtagaccaaa taacagtttt cttcaatcat
    78961 tcctgttttt aatctttcga tatgcatgtg cattcctcct tgatagccaa aacttctttc
    79021 atccattagt ataacgcatt cattgctagg aaacaaaaac aggtacttat ttctctttac
    79081 ttatctgaat tatcagacta tatgaaaagt atttggatat tctaaagaga aggaaagtaa
    79141 gttttcatag cattttaata tgtccttgtt acattagatt catccaaaaa tccaaattct
    79201 atatcctata tccctgccta atcagcaggg tgttttttta tgtttggaaa gagagctctt
    79261 tttgctaaac taggtttttt caggtaatct tagagagaaa gaggtggtgg aaatgatcaa
    79321 acagtcaaaa atatctgtgc ggcacttagt agaatttatt ttgcgacgtg gaagtatcga
    79381 caatcgtaaa aaaagtaacc atactgcatt agaaggagcg agaatccacc gcaagctcca
    79441 aaaagatgcg ggagaaaact atgaaaaaga agtcttttta aaaacaacag ttcaattaga
    79501 tgaatattac ctgatcgttg aaggccgtgc agacggtatt ttcaaaagag acggtattta
    79561 ttatatagat gaaatcaaaa cctccgaacc acgatttgaa gatttagaac cagaacaagt
    79621 ggaacttttc tttcatcaag caagggtgta tgcctatatc tatagtcaag aacatgattt
    79681 acaagaaata aatctgcaat tgacttactt ccagacggca gaagaagtga tcacgagaaa
    79741 ggttgaacat cagacaagag agcagttaga aacttttttt aaaaagctga ctgaagatta
    79801 taaagaatgg cttgtttttc aagaaaactg gcgcactgta cgcaatgcct ctttgatggc
    79861 tttgaaattc ccccatgatg agtataggaa aggtcagcgt gaactagcgg tagctgctta
    79921 taaaacgatc cgtacgaaac agaaattgtt tgtagaagca cctaccggaa caggaaaaac
    79981 aatctctacg ttattccccg ctttgaaagc tgtaggggaa gaagagggag aaaagatctt
    80041 ttatctaacg gctaaaacaa taactagaca agtagctgaa gatgctatga cagcattaaa
    80101 agatacagga gctgaagtaa aaagtgtcac tttgacggcg aaagacaaga tctgcttttt
    80161 agatgagacg acctgtaatc cagatcagtg cccatatgcc aatggttatt acaatcggat
    80221 taatgaaggt ttatgggatc ttttgaatca tgagaatcag atcactcgtg aagtcatcga
    80281 aacttatgca agaaagcata cactttgtcc tttcgaacta tcattagatg tcagtatctg
    80341 gtgtgacgta atcattgggg attacaatta tttatttgat ccaactgttt atttaagacg
    80401 tttttttgaa gatgaaaaaa acgaggacta tctgtttttg attgacgaag cacataattt
    80461 ggtgaatcgt tcccgagaga tgtattcggc agaactatct tatgaaaaaa cgaaacgaag
    80521 taaggaagct ataccaaagg aattcaaaaa acttcatcgc cgtttcaaca aactattaaa
    80581 agaatttgat tcgatccgag aaatagcaaa agaagatcat tgggattatc atcatcaaaa
    80641 agcaccggct gaatctttag taaaggcagg ttatcaatta agcgaaaaaa ttaaagaatg
    80701 gctggcagaa tttcctgagc acccatcaca agaacaattg cttccttatt attttgattt
    80761 gcttcatttt ttgaaggtca gtgaatttta tgatgatcac tacgaaacaa cagtcgaaaa
    80821 aacgtatcgc gatctgatag taaaagagtt ttgtattgat ccaagcttgt ttcttgagca
    80881 gtcgttggat aaaggaaaaa gcagtattct tttttctgct agtttttcac cactctctta
    80941 ttatcaggaa acactggggg ggacaaaaaa gtctggctta caaactgccg agtcctttcc
    81001 cagaaggaaa ccagcaagtt ttcatcgcaa attacctaga gacgacttat cgaaaaaggg
    81061 aacacagtct ggcaaaatta gtagagacga ttcatgcttt tgctgatgga aaggtcggga
    81121 attatttcgt tttctttcct tcttatcaat atttagatct tgcggtggaa gcattcagac
    81181 agcaatatcc cgacagcaat gtgctaatcc aagaaacgga catgaatgag gaagaacgcg
    81241 aacgattttt aacaaaattt caaacaaatc ccgctgaaac attgattgga ttttgcgtac
    81301 ttggcgggat tttttcagaa ggaatcgatt tgagagggga ccgattgatc ggcagtatga
    81361 tcgtaggtgt aggtttgccg cagatgaatc atgagcagga actgatcaaa tcatattatg
    81421 atgagaagga acaccttgga tttgcttacg cttatcagct tcctggaatg aacaaagtct
    81481 tgcaagctgg tggtcgagtg attcgtggca tggcggatca aggaatcgtt gtattggcag
    81541 atcaacgatt ttctagtttt tcttatcgac gtttattccc acaacattgg caaacagcaa
    81601 gagaagtacg aagtgttggg gaattggaag aaggaataca gcgtttttgg ttatcaaaag
    81661 aagcagtaaa tcaaaaaaac gaggaatgac agacgtcagc ctcgtttttt gattaacgat
    81721 agttagtgaa ttgcaactcg attggtaagt caagtttacg taataaagca atcacagctt
    81781 gtagatcatc acggttctta cctgtcactc taatctgatc ttcttggatt tgtgctttta
    81841 ctttcagttt ttcatttttg atagctgtag tgattttctt cgcgttttca cgatcgattc
    81901 cgttgaccaa atcggcagtt tgtcgagctt tgccacctag agcgtgttct gattcggaaa
    81961 aatggatatt tttgattggc acatttcttt tgatcagctt accaaataat acatctttga
    82021 tctgttcgat tttgaaatca tcatcgctca atacaactag attgctgttc tctaatttga
    82081 tttctactgt agaatccttg aagtcaaagc gattggacaa ttcttttgtt gtttgctgaa
    82141 tcgcattttt tacttcttct gtattgactt ctgatacaat gtcgaagcta gcatcttttg
    82201 ccataggaac cctcctgata tacatataaa tatttaccat acatctatct tatcatagga
    82261 ttagggcaga ttcttacctg tttgctaaaa ttcttctcat ttgagttcga agtattttat
    82321 tttaatagat aaactgttat tcttacattt ggaataccaa agtaggggag aggtgcttgt
    82381 gagtaaaatt atgactgcgt atcataaaaa tgaaagatta aagaaaatag tggttatttt
    82441 gataaccgga ttgaccgctg cggtcgcttt aaattatttt ttgattccag ccaatgtctt
    82501 ttctgcaggg atgaatggga tcgctcaaat catttcctcg cttttatatg aatggtttca
    82561 tatcaagata gacacaggaa catttatttt tcttttgaat atccctgtgt ttgtattagg
    82621 gtttctcaaa gtaggaaaac gagcaacgat ccttagtttt gtgaatgttg tatgtgtatc
    82681 ggtgatgacg atgctgcttc cagtcggaca gatcactgat aatattttga tgaatgccct
    82741 agtaggcggt gttttattag gtcttggcgt aggattatct ttaaaaatgg gctttacaac
    82801 tggtgggatg gatatcatct ctcttgtact ttcacaaaca actggaaaaa cagtcggaaa
    82861 ctacatgctg attctaaatg gtatcattgt agtcatcgca ggcttcttct ttaactggga
    82921 aagtgccttg tatacgatta tttccattta tgcaatgact caagtaattg atgccatcca
    82981 cacgagtcat caaaaagtaa cagcaatgat cgttaccgtc aagccagaag aggtaacaga
    83041 agaactagca aaacggatgg tacgtggaat gacattactg ccgtctgtcg gtggctattc
    83101 aaaaaaacaa gggaacatga tcatgatggt catcacccgc tacgaaatgt atgatcttga
    83161 tcagatcgtt catgaaatcg acgacgatgc atttatcaat attttaccaa ctcattcagt
    83221 atttggccgg tttgcaaatg aaaacgaaca aaaaatgtat cgaacaactg gcgtattgcc
    83281 agaaataaaa aatcgttcac aaaaaaaatg aggaatcagg tttattttag aataaattta
    83341 atctattaca tctaataaga acatttttat aaatttaaac gagaatctta cttctaaatt
    83401 ctcatttaca agcagctaaa aaaagagtat gataaataaa aatcgtgtta aggagtgtcg
    83461 tacgatgagc gaagcaaacc aagaacggtc tggacaagaa atagaactaa tcaaagaacg
    83521 tattcttgct gccttggaaa tggtgattga tcctgaatta ggaatcgaca tcgtcaattt
    83581 aggcttgatt tatgatatag aattcaatcc tgaaaatgga gaaacagtca ttaagatgac
    83641 tctaacaact atgggctgtc cattagcaga tattttaaca gaatctatcc atgacgcatt
    83701 aaaagaagtg ccagaagtaa cgaaagcaga agtcaaacta gtctggtatc cagcatggac
    83761 gacagataag atgagccgtt atgcacggat tgcgttgggg atacgataga aatcttggct
    83821 gactagctat gagaagcaaa taggaaaaac aaaaaagcct tttatccttt ctagaagaaa
    83881 agagataaaa ggtttctttg tttataaaat tttgtctgct atattataag ttaattaaat
    83941 aaatgtgttc tattgataaa ggacattcaa atccctttcc ttttggaatc cttctttttt
    84001 acatcagttt gattttcgta tagtattctt tctcatcttc acgataaatc gcagccccat
    84061 ttttttgttg tgctttaaga ctgccaagat tttttttatt tacagacaaa agtgcctcat
    84121 caatgccgat tttttttgcc tcttgtagta ctaatgctaa tccttgtgtg gcatatcctt
    84181 ttttacgata attaggggag atgccgtaac caatatggcc agcgccgttt cttaatgttt
    84241 cgttcagttt atggcgcaaa ttaaaaattc ctacatactc atgttcatca ctttctaaaa
    84301 taaagacggt tgcgggaacg taactctcag gaagatcgat tcctaaagac gttttttcgc
    84361 atttttttac ataatctaaa aattcagcaa atgtatagcc ataagcattg ttgataaatc
    84421 cactctcatt tggtccaaag ctagtttgta gctgataagc tttctttaaa tctgcttccc
    84481 atagtttgat cagtttcatc gtattcacct ctttaagtaa ccttattcat ttgttttcta
    84541 ctatagcact attgttgtaa atagccatgc ctatttttat ttattgtgtc atatcgtatt
    84601 aagaatcatc gaacaataca aatatgaatt acaataaaaa aattatttga agttcgttca
    84661 ttcgttactt atgatctcta gaagaaagaa aacgtgaatt acttcagcgt ctagaattcc
    84721 agaaggatag taatcaacag aagaaataat caatatccaa atgaaagaag atagctgaat
    84781 cgagaaaaag aggaaaaaag aaatgaagaa aaaaataaaa tagttactaa tcccagagtc
    84841 gttcgtttaa cattgaaatc gatcatatgg ataaaacaga ttttttcacc aaccctcaat
    84901 agatgatgga gttgctcgga tgaattattt gtgtagatgg cataagggag ggcgtacaga
    84961 aatgaaaaag aacaaaaata tgttcttgac aaaaaacaca tgtaatccta aactaaaaga
    85021 tgtgatgcgg ctatggcgga attggcagac gcgctagctt caggcgctag tgagggtgac
    85081 ctcgtggaag ttcgagtctt cttagccgca taatttgttt ttttataaag agaaggtaca
    85141 cctggagcga tgagaaagaa aataacatat tatctaaaag aatataggaa aaattttaaa
    85201 caacggaacc agattcaaat aaatcaaaaa aaatttgtgt gaaggtggga cagtttaaaa
    85261 cctaatagat tgattcaaat agtagattta tagattctga tacaagaaat actggcaaac
    85321 gatagttaag gaataaagac taaacttaaa ctcaaaaaga ggtcatgcga aagaataaat
    85381 cgcatgacct cttactatat tttgaatgtg tttaaacgtt caaataatga atcttagtac
    85441 caaccgtttg cttcccagaa tgctttcgcc gcttcccaag aaccataacg tgaagtaaca
    85501 tattgttctg ctactctttc ttggtttgca gcagagtagt caccatttaa gtaagaagaa
    85561 tctaattggt aacggccgat gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct
    85621 ttttgtgcga tccattcttt tgcagagctt gtacttgctg gagtagcagc agtttctgtt
    85681 acagcaggag caggttgtgt ttcagcaaca ggcgctgctt gtgtttccgt ttgaaccgtt
    85741 tcttgtacaa caggttgttc agcttgaaca ggttcagctt ggactggtgc ttcttgaacg
    85801 gctgcttgag gttcagcagc tgcttgtgca gagccttctg ttgggattgt taattgttgt
    85861 cctgagtaga tcaggttaat atcagaaatt gaatttgatt ctgcaatggc attgatcaag
    85921 gagttatcac caacgtattt ttgagaaatc gttgatagag tatcacctga ttgaacggta
    85981 taagcttcgt ccgcatgagc agtcgttccc atgaagaatg cagcgccggc agccaaagtt
    86041 gttccaaaaa gtaaagtttt aagtgatgtc atgaaaaatc tcctttattc gttaaaaagt
    86101 tttatttgtt gtgttcaacg aggaatactt taacacgtga aaatatttca taggtaaaag
    86161 aagcatgaca atttatgaca aatccacgtt ttttgtaaca gaatgatgtt cgctcgatgt
    86221 gaaaaatgat gaggaatttc acataatgca gaaataatgg gattagaaca ggtgttctga
    86281 tgagaaaaga aatgtataaa taacttgaaa gaaattgctt ttgttcatca ttttatttac
    86341 atattgcgta tattttttgt atttttgctt gagatgttac aataaaagaa aaaggaggcg
    86401 gttccaatga gtaaaagata ccagaacatc atggtagcag ttgacggatc aagacaatcg
    86461 atcaaagcgt tcgctgaagc ccttgactta gcaaaggaca atgaagccca cttatttatc
    86521 gtgtcaatca tcaacaaagt tgagctgacc catagtgcct atgccttttc aaaaatctat
    86581 gcagatgaaa aacaaagaac agaagtagcc atgctgaaaa aaatcaatga tgccaaagag
    86641 tacggcattg ctgagatcca cgcaatagtt gaaactggag atccaagaaa tttgattgcc
    86701 aatgtcatcc ctggacaaga aaatattgat ttgatcgtta tgggagcgac aggaaaaggt
    86761 gctatacaac aagcattagt tggatcgacc gcttcttatg tcgtatcaca tgccccttgc
    86821 agtgtgttgg tcgtgaagta atgagataag ggaggaatag aaaatggttc gtcctcaaga
    86881 agtccgtgcg ccaaaagaaa aaatagaaat tttagcaatt attgaagacg gcacacaaac
    86941 aaagaaggga tactcgatcg ctttagtaaa gtggaaagga aaaaaaggtg ttgctctgag
    87001 atgggatgga gataaccaac aagataaagg attccctgtc acagctaacg gttatcatcc
    87061 agcgtggttt gttctgcctg ataaatttat ggaattatac agctatgatt atgcaagagc
    87121 cgcagcatca atcaaagcat tagataaatt aacagaagag cagaatgaaa tggaggaaaa
    87181 ataaaaaaga cgaaccaatc agcctttaga aaactgatta gttcgaggag taaaaatgaa
    87241 aaaaggttat ttgggatgaa actagaataa tgcgtataac ttaaataaaa cttaaagcaa
    87301 taaaatggca agagggtgag gcaaatggaa gcaggaaaag tattacagga agtaaaagca
    87361 ctatgtgcag ataaaaaata tgaagaagcg aaaagcctga tcgaaaagaa caaagaaaat
    87421 ttggaagaaa agtacccgat cgcacaagaa tttatagaat taaaacaagg aacgatcctt
    87481 caccgattca aacatttttt tggtataaca gaatagaaaa aacgattaaa gatcggcaga
    87541 aatttgctgc atctttaatc gttttctttt taaacatgcg agggattcaa ttctgattct
    87601 tatataaaaa atagatccaa ataataagaa aaattactga aagcgtcaat tcgatgatgt
    87661 gtgttattaa tacagacgtt gtttcccaat gcctaaaaga aaaaatttga taagtaaaat
    87721 atacagcagg taaagaggcc agtaaaactg aaaacttctt ttttgtccat tttatgtaat
    87781 agaaaagaat gcttatcata ataaaaaaag gaatataaaa aacaaattga ccgatcgcga
    87841 tcatactatt gcctccgata aataaagtcc atgatacaaa ataacacttt gtgcgaaatg
    87901 gcgcaagcag ttttgtggat ttatccggta aaatctgtat cgattttgaa attagaggag
    87961 aaacttgcta taatcaaagt gaaagaagga gtgggatttt catggatcat gtagtgttag
    88021 aaatcgattt gcatttgatc aataatattc gggtgattta ctatgttgta agtaacagtg
    88081 tcgaacaacg tgtactgacg aataaaataa atggtatttt agcaaagaaa gacgttcacc
    88141 gattcaataa tggtgaagga agttattata gtatcccagt agaaaaaatt atttatacga
    88201 cagtcaaagt cagagaagat ctagatacca aaaaagcata tgaacctatt tttacaactt
    88261 attgattgac agcagatgga ggaaaaaaat gaatccatat gaacaactta ttcaagattt
    88321 gatcgatgga aaaatcgaaa agattgatgt aaaaagagaa gaattaatgg cttttcgaga
    88381 agcgtggctg aagttggaga atcgtaaata tgtggtggga aaagcctcac tgaacggcaa
    88441 tgtgacttat cattacgatc cgacacgttt attttagtat gaagtattca gaaaagaacc
    88501 aaaggaggac gatgcagcag cattgtcctc ctttgatttt acaaaagtga aagttttact
    88561 tatttgaata gatttttaaa tttgtttttg acactgtcga tatttacatc ttttaaaaga
    88621 ttttgggctt tctctttata aggtcctaat tgttctttat gttcttcgat aaatcgtttt
    88681 gcgccatcga aattgccgtt cttaatcatt tccttgactt tttgaaataa ttcttcttga
    88741 ttcattttga acacatcctt tgctatcaac atgatttttt acatggacaa aacgagaaaa
    88801 actgatttat ccacacgtca ataatatcaa ataacgatca tttttactaa aaaaacactc
    88861 gattctcgct tctaaagtag gagaaaaatc tccaacttgg gacggatgga atttctttaa
    88921 ctgacccata tactgaatat ataacaagga ggtagaaaga tgaaagtgat taaatatttg
    88981 attggactgg cggcgattag tggtattttg ctatttggac tgaaatatta tactgaagga
    89041 agttatggag aaatccctgg tatacttgat cagctaaatc cattagttga aaaaggagag
    89101 gtctatgtaa agacacaaaa accagaggaa gtaaatgaat atggtacagc aaggtatgtg
    89161 cagaaagctg ctgatgccaa tgggaaagaa cgaacggttg aatttaatgg tctttctgtg
    89221 ttaaaagaaa atcattattt gaaaattagc aataaaggag cacacataga aacgtatgag
    89281 gaagttccta gagaaaaagt tccggagaaa gcattagcaa ttattgggta aatcagtttt
    89341 tgtattaaga atacgcccga ttttattatt cataatttaa ttatgtaaac taaatacaag
    89401 tagtaaaaat aatacaataa tccccctgca aaaagattgt tcttttacag ggggttcatt
    89461 ttttcagcaa gcacatcaaa ttgggatggc agggttcgaa cctacaattg cttgattcag
    89521 agtcaagtgc cttaccagtt tggctacatc ccatttattc aacaaaataa tcatagcact
    89581 ttcctaaaaa aacgcaagtt atttttcttg atcggatagt tgttgcttta ctcgtaaaag
    89641 gcggcgtact agtttctttt ctaatccagc aggaagggca atcagttctg ctactgacaa
    89701 gacggtgtcc aataactgat attctttgac aggttcattc ataatatgat agatgtttgc
    89761 taaacgttca atatattcaa agcgagtata ctctgttcga gcaattagaa atttcaataa
    89821 atcggtggct ttctcgtact cttgatctgc gtaataaaca agagattgac gatacaattt
    89881 gatttccagt tcattgaggg tagcaggtct gcgacgctcg ttccactcat taatagagac
    89941 tttaccttga acatttccct tgcagtcacg gacaaaaata atcggaattt catttggatc
    90001 cattttttta tgtgtgaatt tttccattat ctacgctccc ttcaaaggtt tggtatcttg
    90061 agtataaggg atttttgttt gaattactag cttttctcca aagttagcat ctgtccaccc
    90121 attgcagtga aaatcgccga aaaatcgttg atcggtactg ctcgattttt tacaccatag
    90181 ggatcgttga cataaatatt ttctttgtca tatccagtga ctacacaagc atgaacaaga
    90241 ggagtgactc ttacttctcc attatttgtg gtccaagtca taaaatcagt ttcatctggg
    90301 acttcaaaat tgatagtcac agaagtccag acaggatttc ctttagatag tgcttttttg
    90361 atatcatcga gttgagcatc ggtttttgga aaagtgtgat aatccgtttg gacgatttgc
    90421 tgagcaaggg aggcaactgg gggaacatag acgaccatag cccagtctcc gccactgata
    90481 tcaccaacaa atccttcatt aggatcgcca tgagtcccat ctgcattgaa aacagggacg
    90541 taatccaatt gctcagctaa ctggttttta gttgtttcat aaccataaaa agataaaagc
    90601 atagaaagcg aagtgacttc acagccattt tctaaagaag gagaatcaaa ctggctctct
    90661 aatggaacat ctagtaaaac ttctgctttc tcttttgtct ttttcgtttt ttgtgttgaa
    90721 cttttttgtg cagtgttcat tgtatgttct ggagaatgga tatgtaggta ataacttgta
    90781 ccgcttaata aaagagcagc tgtgccccct agaaacaata gttttttgaa cgtttttttc
    90841 ttcatttgaa atctcccttt tcagctgttt atctttccta ttataaatgt atttttattg
    90901 gaaatggaag tattatattt taatatgtag gaaataatta gtttttaagg aatatgtttt
    90961 aattttttga aaaaaattct tctaaataca acagctgttt taattgtgaa aacgaaaaca
    91021 gtacgctata atgcctgtaa ggttgatttt taactgtaaa taaaaatcaa tgagttaaat
    91081 ctccaaagaa atagcagtgg taatgtggag aaaagttcca gcagtttagg aggattttga
    91141 tgacggaaga aatgattaat ttaggagaac aatatgcatg caaaccaatt ggttttacaa
    91201 aaacggtcat tggtgaagtt gtatcaaaaa tgacaaactg cgccgtggtc aaagttgccc
    91261 aatgtgctgc agaggaccaa gaattattag atgaaaaagc aagcatggtt gttgcaaaat
    91321 atgatacatt tgaataaaga aaacaattgg acgtgcggcg tgctgcaagt ccaatttctt
    91381 tgagaaggta aatcagtatt cttttcctat tttctggaga agaaaaagcg aggcgagttg
    91441 tcatggtaac attctttacg gttctattgt ttttattcgt tgttgtgatg tttttatttc
    91501 tttggctggt tcgaaaagaa attatttatc ggactgtgcg aaatcgatgg gtctacttgg
    91561 tgataccctt cttagtgtca ctagtcattt ggtatacact catcagtcat cctacagcag
    91621 atgaattggc aaaagggatc ttgtcagcaa tgatttttat tagttttttg ttggacagta
    91681 gaggaatcac agaagaagga cttgttttga atagctttga caaaaaaggt gttccttttt
    91741 ctgagatcaa taaaattgta ttatatcagc caaaaggttc taaaatcgta aaaatgaatt
    91801 ttttccgaaa cggatggcga ggaccgatgc tgaaattttc tgcatcatta gaagaattag
    91861 ttccattttt gtcccaacgc ttaaacgatg aggcagagat tgatattatg attgatccag
    91921 aataaaaaca gaggttgcga caagatgttt gtcacaacct ctgtttttta tttcaaataa
    91981 aattatccaa atacgcggaa gcgatcggca tcgttcatgt attctttttc cccagccggt
    92041 gtttctggtg aaccgaatac taattgcgaa cgtagtttcc agtttgaagg gatattccat
    92101 tcttttgcaa ctgcttcgtc aataactggg ttgtaatgtt gcaagtttgc gcccaatcct
    92161 ttatcaacta atgctaccca agtattagct gttgcgattc cgtttgattg ttctgaccaa
    92221 tctgggaagt tgtctgcata taattcaaat tgttcttgta gaccttttac cacgtcagta
    92281 tctttgaaga ataatactgt accataacca tttttgaaac cagctaattt gttttgtgta
    92341 tttgggaaag cttctgctgg agtcaatggg cgaagtgctt cttcagtgat ttcccaaagt
    92401 ttttcatgtg catctccgaa aaggataact gcgcgtgttg attgagcatt gaaagcagtt
    92461 gggctttctt taattgcttc tttgatcaaa gttgttaatt cttcattaga aagtgaaaca
    92521 ttacgtccta gatgatagat tgaacggcgg tttttcaaag tgtcagtaaa agttgtcata
    92581 ataaaatttc tcctttgatt tgcgttttgc atttttttaa ttgattacga aatgaagtat
    92641 agcaccttac aaaaagtaag taaacagata tgctcacaaa aaataagcga agttcaaaaa
    92701 acaattttaa taagaaaaat cgttatgtaa ggattttctt agttatcgct ttcattgt
//
LOCUS       DAJSKS010000002        68372 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJSKS010000002 DAJSKS010000000
VERSION     DAJSKS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.002"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            164..1045
                     /locus_tag="ON396_000095"
     CDS             164..1045
                     /locus_tag="ON396_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3112234.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1058..2668
                     /locus_tag="ON396_000096"
     CDS             1058..2668
                     /locus_tag="ON396_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HCR3112235.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2745..3404)
                     /locus_tag="ON396_000097"
     CDS             complement(2745..3404)
                     /locus_tag="ON396_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR3112236.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3687..4352)
                     /locus_tag="ON396_000098"
     CDS             complement(3687..4352)
                     /locus_tag="ON396_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HCR3112237.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4499..5104)
                     /gene="dhaL"
                     /locus_tag="ON396_000099"
     CDS             complement(4499..5104)
                     /gene="dhaL"
                     /locus_tag="ON396_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCR3112238.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5132..6142)
                     /gene="dhaK"
                     /locus_tag="ON396_000100"
     CDS             complement(5132..6142)
                     /gene="dhaK"
                     /locus_tag="ON396_000100"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR3112239.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6117..6563)
                     /gene="dhaM"
                     /locus_tag="ON396_000101"
     CDS             complement(6117..6563)
                     /gene="dhaM"
                     /locus_tag="ON396_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCR3112240.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6666..6986
                     /locus_tag="ON396_000102"
     CDS             6666..6986
                     /locus_tag="ON396_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR3112241.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(7098..7967)
                     /locus_tag="ON396_000103"
     CDS             complement(7098..7967)
                     /locus_tag="ON396_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3112242.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(8063..9367)
                     /locus_tag="ON396_000104"
     CDS             complement(8063..9367)
                     /locus_tag="ON396_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3112243.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9364..9873)
                     /locus_tag="ON396_000105"
     CDS             complement(9364..9873)
                     /locus_tag="ON396_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112244.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(10048..12183)
                     /locus_tag="ON396_000106"
     CDS             complement(10048..12183)
                     /locus_tag="ON396_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3112245.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12266..13921)
                     /locus_tag="ON396_000107"
     CDS             complement(12266..13921)
                     /locus_tag="ON396_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3112246.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13923..14237)
                     /locus_tag="ON396_000108"
     CDS             complement(13923..14237)
                     /locus_tag="ON396_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR3112247.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14407..14667
                     /locus_tag="ON396_000109"
     CDS             14407..14667
                     /locus_tag="ON396_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HCR3112248.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14681..14995
                     /locus_tag="ON396_000110"
     CDS             14681..14995
                     /locus_tag="ON396_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HCR3112249.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15725..16279)
                     /locus_tag="ON396_000111"
     CDS             complement(15725..16279)
                     /locus_tag="ON396_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR3112250.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16405..18334)
                     /locus_tag="ON396_000112"
                     /pseudo
     CDS             complement(16405..18334)
                     /locus_tag="ON396_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18640..19356)
                     /locus_tag="ON396_000113"
     CDS             complement(18640..19356)
                     /locus_tag="ON396_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCR3112251.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19357..21180)
                     /gene="glpO"
                     /locus_tag="ON396_000114"
     CDS             complement(19357..21180)
                     /gene="glpO"
                     /locus_tag="ON396_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HCR3112252.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21194..22690)
                     /gene="glpK"
                     /locus_tag="ON396_000115"
     CDS             complement(21194..22690)
                     /gene="glpK"
                     /locus_tag="ON396_000115"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR3112253.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22826..24286)
                     /locus_tag="ON396_000116"
     CDS             complement(22826..24286)
                     /locus_tag="ON396_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3112254.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24276..25571)
                     /locus_tag="ON396_000117"
     CDS             complement(24276..25571)
                     /locus_tag="ON396_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3112255.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25903..26580)
                     /locus_tag="ON396_000118"
     CDS             complement(25903..26580)
                     /locus_tag="ON396_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HCR3112256.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26610..27701)
                     /locus_tag="ON396_000119"
     CDS             complement(26610..27701)
                     /locus_tag="ON396_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCR3112257.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27802..28236)
                     /locus_tag="ON396_000120"
     CDS             complement(27802..28236)
                     /locus_tag="ON396_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3112258.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28820..30277)
                     /gene="ascB"
                     /locus_tag="ON396_000121"
     CDS             complement(28820..30277)
                     /gene="ascB"
                     /locus_tag="ON396_000121"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR3112259.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30274..32169)
                     /locus_tag="ON396_000122"
     CDS             complement(30274..32169)
                     /locus_tag="ON396_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3112260.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32227..34002)
                     /locus_tag="ON396_000123"
     CDS             complement(32227..34002)
                     /locus_tag="ON396_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR3112261.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(34052..35548)
                     /locus_tag="ON396_000124"
     CDS             complement(34052..35548)
                     /locus_tag="ON396_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3112262.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35742..37364)
                     /locus_tag="ON396_000125"
     CDS             complement(35742..37364)
                     /locus_tag="ON396_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR3112263.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37692..39005)
                     /locus_tag="ON396_000126"
     CDS             complement(37692..39005)
                     /locus_tag="ON396_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCR3112264.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39250..39474)
                     /locus_tag="ON396_000127"
     CDS             complement(39250..39474)
                     /locus_tag="ON396_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112265.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39494..39916)
                     /locus_tag="ON396_000128"
     CDS             complement(39494..39916)
                     /locus_tag="ON396_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HCR3112266.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(40058..40768)
                     /locus_tag="ON396_000129"
     CDS             complement(40058..40768)
                     /locus_tag="ON396_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112267.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40786..41283)
                     /locus_tag="ON396_000130"
     CDS             complement(40786..41283)
                     /locus_tag="ON396_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3112268.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41320..41772)
                     /locus_tag="ON396_000131"
     CDS             complement(41320..41772)
                     /locus_tag="ON396_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3112269.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41907..42512)
                     /locus_tag="ON396_000132"
     CDS             complement(41907..42512)
                     /locus_tag="ON396_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR3112270.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42962..43582)
                     /locus_tag="ON396_000133"
     CDS             complement(42962..43582)
                     /locus_tag="ON396_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCR3112271.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43584..44453)
                     /locus_tag="ON396_000134"
     CDS             complement(43584..44453)
                     /locus_tag="ON396_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HCR3112272.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44476..44883)
                     /locus_tag="ON396_000135"
     CDS             complement(44476..44883)
                     /locus_tag="ON396_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR3112273.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44902..46191)
                     /locus_tag="ON396_000136"
     CDS             complement(44902..46191)
                     /locus_tag="ON396_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3112274.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46218..46520)
                     /locus_tag="ON396_000137"
     CDS             complement(46218..46520)
                     /locus_tag="ON396_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3112275.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46545..48638)
                     /locus_tag="ON396_000138"
     CDS             complement(46545..48638)
                     /locus_tag="ON396_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR3112276.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(49127..49891)
                     /locus_tag="ON396_000139"
     CDS             complement(49127..49891)
                     /locus_tag="ON396_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HCR3112277.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(50002..50871)
                     /gene="mdcB"
                     /locus_tag="ON396_000140"
     CDS             complement(50002..50871)
                     /gene="mdcB"
                     /locus_tag="ON396_000140"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HCR3112278.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(50896..51972)
                     /locus_tag="ON396_000141"
     CDS             complement(50896..51972)
                     /locus_tag="ON396_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR3112279.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(51996..52589)
                     /locus_tag="ON396_000142"
     CDS             complement(51996..52589)
                     /locus_tag="ON396_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HCR3112280.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52593..54269)
                     /gene="mdcD"
                     /locus_tag="ON396_000143"
     CDS             complement(52593..54269)
                     /gene="mdcD"
                     /locus_tag="ON396_000143"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HCR3112281.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54259..54558)
                     /locus_tag="ON396_000144"
     CDS             complement(54259..54558)
                     /locus_tag="ON396_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HCR3112282.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54660..56306)
                     /gene="mdcA"
                     /locus_tag="ON396_000145"
     CDS             complement(54660..56306)
                     /gene="mdcA"
                     /locus_tag="ON396_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HCR3112283.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56313..57224)
                     /locus_tag="ON396_000146"
     CDS             complement(56313..57224)
                     /locus_tag="ON396_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR3112284.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57344..58201
                     /locus_tag="ON396_000147"
     CDS             57344..58201
                     /locus_tag="ON396_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3112285.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58388..58750)
                     /locus_tag="ON396_000148"
     CDS             complement(58388..58750)
                     /locus_tag="ON396_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR3112286.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            59096..60535
                     /locus_tag="ON396_000149"
     CDS             59096..60535
                     /locus_tag="ON396_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3112287.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(60542..63094)
                     /locus_tag="ON396_000150"
     CDS             complement(60542..63094)
                     /locus_tag="ON396_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HCR3112288.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(63208..64539)
                     /locus_tag="ON396_000151"
     CDS             complement(63208..64539)
                     /locus_tag="ON396_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3112289.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(64505..65983)
                     /locus_tag="ON396_000152"
     CDS             complement(64505..65983)
                     /locus_tag="ON396_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3112290.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(66288..68123)
                     /gene="tdc"
                     /locus_tag="ON396_000153"
     CDS             complement(66288..68123)
                     /gene="tdc"
                     /locus_tag="ON396_000153"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCR3112291.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        19472 a        14573 c        11689 g        22638 t
ORIGIN      
        1 aagctagcaa ttccttgttt atgaattcat tgacagaaat ttataaaaag aatattatgt
       61 actaaaataa taaaaaaaag ttttataaaa aggtataaaa cctttttatt aaactattta
      121 atcttgttta gtatatattt ttagaaaaag gagtggggtt tttatggcag aaatcgtcaa
      181 agtcacacat ttgaaaaaga gctttggtcg tttcgaagct ttaaaagatg tctctttcac
      241 cgtacattca ggtgaagtcg ttggatttat cggaccaaat ggttctggaa aatccacgac
      301 gattcggaca cttttaggga ttataaaaaa agacggcgga acggcaacga tttttgggca
      361 agatgtatgg gaagatagtt tatctatcca tgaacgtctt tcttatgttc caggcgatat
      421 ctctttgtgg ggaaatttga ctggaggagc gatcatcgat ctcttcatga agcttcatgg
      481 aaaagggagt gctcaaaaaa aagatgaact gattcaacgt ttctctcttg atcctaagaa
      541 aaaagcaaaa aactattcaa aaggaaatcg ccaaaaagtg ggcttgattg ctgctttggc
      601 tgttgattcg gatctctatt tatttgatga gcctacttct ggtttagatc ctttgatgga
      661 aacgattttc caagaagaag ttgaaaaatt gaaagaacag ggcaaatcca tcctactttc
      721 ttctcatatt ctcagtgaag tcgagcgact agcggataaa gtcgtgatca tacgcgaagg
      781 aacactcgat gaagaaggaa cactcgatga attgcgtcat ttgactcgtt cgaccatcac
      841 tatagaaaca gaagcagaga atagtcactt agcacaattg aacggtgtcc atgactttgt
      901 gcaaaaaggt cacctcgctc aatttgctgc tgataacgaa gctttcgatg tgattttagc
      961 tgaagcgtcc aaattaggtg tgaaaaaatt tgaatctatt ccaccgactc ttgaggattt
     1021 attcatgaga cattatgaag gatgaggagg aagcgaaatg actgataaat tttcacgctg
     1081 gtccaccctt ttttctcaaa accttagccg tgactggaaa aagatcctga tatggctagt
     1141 cggtgtcact gctttttctg gtggttttgt ccctgcgttt gaagaattga cttccggaca
     1201 aggattagct ggattatacg aaaccttgca aaacccggca atgattgcaa tggtcggacc
     1261 aactccagct tcgaatgctg ctgagtatac tttaggagcg atgtatgcaa atgaaatgtt
     1321 gcttttttgc agtttgtttg ctgctgtgat ctctgtttta cacgtggtga gccaaacgag
     1381 aaaaaaagaa gaacttggtc tgattgaact tattcgttcc tttcgagtcg gacgtcaagc
     1441 aaactcttta gctgtcatcg ctgaaagtat tctgatcaat ttgcttttag caatatcgat
     1501 cagctgtacg atgatttttt tccaagcgga ttcgatcact gcagaaggtg cttggctttt
     1561 tgggctatct gtaggtagta tggggatttt ggggagtgcc gttgcactgt ttgctgcaca
     1621 actgatgcca agttcagcca gttcgaatgg tctgtcttta gcgtttttag gtggacttta
     1681 ccttctacga gcaataacag atgtagctga ttcctccctt tcgatgtgga atcctttagg
     1741 ctggtcttat ttgacctatc catttacaac taacaactgg attcctttgc tttatttgtt
     1801 tttgttcagt ctaatcttga taatcagttc atttgtttta gaggaacatc gagatttgaa
     1861 caccggctat atccccgaac gtgaaggaaa accgtatgct aaacgttctc ttttatcctt
     1921 gcgtggttta ttattctatc taaataaagg gatgacaatc agttggctta tcacttttgg
     1981 tttgatgggg gcggcatacg gctctatcta cgggaatatg caacagtttt tagaaagtaa
     2041 cgacttaatg aaacagatgt ttatccaatc tggcgtctca attgaaaaaa actttacaag
     2101 cacgatcatg gtagtcttga tttgtctagc tgctatttta ccaattgcta gcatcaatcg
     2161 tttatttaca gaagaaaccc acggacgtat gaatcagctg ttcgcaacaa aaatttctag
     2221 gaaccaactt tattggacca atatgttact agctattcta tttgctatat tggcaatcct
     2281 gattagttct ggtgcactag ggctttctgc tttggcttcg atgaaggatg cgaaagacat
     2341 ggatctattg atgtttctta aagccgggtt caatttcctg cctgccgttt tagtgtttat
     2401 cggaattagt ggctttctat tcggatggct tcctcgaatc ggtaaacttg cttatatcta
     2461 cttagcttac tcctttgcac tcaattactt tggcggaatt ttggatttac cagaatggtt
     2521 ttccaaaaca gctgtcccta gctgggttcc acggatgcca aacgattctt ttgatctttc
     2581 cgtgttcctt acgttatcat gtatcagcct tgcactgttc gtccttggtt ctattggtta
     2641 taggcgaaga gacttactag aaggataata aaaaagcatg tacaatttca tgctagcctc
     2701 caaatctggg tcaaaaatcc aacatttgga ggtcattttt ttatttacaa gtcttctaac
     2761 tgctctttat caatgatcca gacttctcga ttctttcttt ttacataacc attctcctct
     2821 aataatttaa attttctaga taaagtttcg ggggtcgtac cgataaatgc agcaagttct
     2881 ttcattttca ttggcaggaa aaagtgatcc ttccctgtcg ctttagctaa gtcaagcaga
     2941 tagacagcga ctcgttcttc tactttttcc atcatcaaaa aagttgtctg ctgctgtact
     3001 tttgttaatt tctcaacgtt gattgctaaa agtttcagac taagggcggg ataatgcagt
     3061 aagagttccg agaaatcttt ttgtctcaat atgcaaattt ccgttttttc tagtgcttcg
     3121 ccaaataaag actcatttgg aacgcctagc aactggctct ctccctcata tccgcccggc
     3181 tctattactc gtaaaagctg ttcctttccg ctagcagata attgatacac tttcatactg
     3241 ccggaggcca cgatcactaa ttgaggttct ccattcggcg caagaatctg ttctcctttt
     3301 tccacatagt gatgtttgac aagtgcgtgt actttttctt gatcttgctg tactaagtga
     3361 ttgaacaaag gaacaaatga aacacataaa tgatcatgac tcataatctc tcctccttct
     3421 aaacagctat ttctaataaa gaaacataac ttcccttatt ccaactgata attttctttt
     3481 atcatagcat aaacgtcaag cgaaccagat accagcaaat agagtggatt tctttttgtc
     3541 ccttgcttta ttttccttct tttgagacct taagtaattg attcctatca attttttaaa
     3601 tatttacttt ttgaataatc aaaaacaaac cttcactcgc aagccgagag taaggtttgt
     3661 ttttcgcatt gcttatttcg tgttgtctat tgaaattttt ccgcacctag cttttgatat
     3721 agtggtgcta gttcttgtaa ggtttcagcg gttatttgct caacgtgttc gatttcttgc
     3781 ggatagaaga atagtttacc catttgaaag gttcggtctc tcatcgcttt tttcaactct
     3841 tcttgtttct ttgtatcgga caaatagtca gacaaagcta agtggtcgtc aagttcatgt
     3901 tctggttgcg gccatatgta ataatccttg atatcgactg ttttgctcca ctctttcagt
     3961 atcgcaagca actggttata cgctgcaatg ctaccatatc gttcttcgct tttataatgt
     4021 tggtacatca aatagatttg gtattgtttt tgattcaaca aaactgctag acaggcattc
     4081 tcgtcttgac gagattcgct tcggtacgcg ctccaaaaat gactgcgtaa attccagcca
     4141 ttcgtccagc tttcgatttt aggtttgctc atgccgtaca tatcaggcaa aagtgcagct
     4201 gtctggagct gaatctcttt ccatttttgc cagtgttctt tatattcttt cttgatttgt
     4261 tcaatcttat ctgttggatg gctctctttg agctgtttga atgcgtagat tggtcgatca
     4321 aacaactgat aatctgattt tccaagttcc attgtttcgc ctctttccgt tatcttttgt
     4381 attctacctg aattttgcga tatggacaaa tagaggttgt gaaaaagctg ttctgcatca
     4441 agtcataaga acagccaaac aaaaaactgc gatgagcatc accgcagttt tattgaactt
     4501 aaggttgatt gatcacatct aaaagcgttg aaaataaaat ggcactagaa gcagctcctg
     4561 ggtcgatatg tccgattgca cgctctccta gataggaagc tcgtcctttt ttagctacca
     4621 agactttggt gtgttctttt gcctggtcaa tgatttcttt tgttaaattc cctgccttca
     4681 atgcctctgt gactggcagc caaacatcta acatcgtttt atcgtcagct tctgcctttc
     4741 ctcggctttg aatacccgct gttccctgct cgatcacttc gcctaatttt tcttgagaat
     4801 caatagaatc gacctctttt gtcgctttca tcatgttcat gaatgcagat ccgtataacg
     4861 gaccagaagc cccaccgact ttcgatatca tcgccataga gaatactttg aatgtatcgc
     4921 tgattgtttc aggatgatcg gcttgaaaag cttcttcata agctgaaacc cctcttccca
     4981 tgttatttcc atggtctcca tcgccaatcg gggtatctag atcactgaga tattgtttgt
     5041 tctgattgat tgcctctgca aattgcgtga gccattcttg gatttcttga acggttagtt
     5101 tcatattatc acccttttat cttgagattt tttaccatga aatcgtctgt acattgcttt
     5161 ctaaagaatc tttccattgt gtctccgtca agtcgatcag tgttagagaa agaccttgca
     5221 tatctagtga ggtcataaaa ttaccgactt ttcggaaaac tacattgacg cctctttctt
     5281 ccaacagagc taaaacatca ttcatgaaca cgaactgttc catcaaaggc gtcccgccca
     5341 ttccgttgac tagtacacct gcttcttttg gtaaattcga gtaatcatct aaaatcttag
     5401 taacaagttc ctttgccagt actttagaag gctgcatctt ttctcgacga tagcctggct
     5461 caccgtggat accaacacca tactcgatct catcttctcc tagttcgaat cctggtttcc
     5521 ctacttctgg tacagttgct gctcgaagcg ctagaccgat cgtcttagtt gcctgtacga
     5581 ccttttctcc cagttctttc aactcagcta gggaagcacc gtttcttgct gcatcgccca
     5641 cgattttgtg tacaaggaca gtacctgcaa ctccgcgttt tccagcagta tacgtactat
     5701 tttcaacggc gatatcatca tctacgatca cactttctac ttcaatatcg tccatagcag
     5761 ctaattcctt tgccatatcg aagttcaatg catcccctgt ataattctta acgatcagca
     5821 aaactccttt tccactatcg gcttccttaa tagccgtctg aatctgatct ggagttggag
     5881 aagtaaacac atcaccaagc accgctgcac ttaacatccc ttcccctaca aacccggcat
     5941 gggatggttc atgtccgctt cctccaccag aaaccaaacc aacttgttcc gctttctgat
     6001 tcttggctac aacacgggaa gattctacac gatgaacaag ttctggatag ctttttacta
     6061 atccttccag catttcttcg acaatattgc ttggatcatt catgattttt ttcaatttat
     6121 tcccatcctt tctttccggc attttgtgct tctgttaaga tttgctgagt atcatctgtt
     6181 ataccggcag tgatggctgc tgcaaaagct ccttccacta aaggcgcatc caccagatga
     6241 taacgttgct gctgctcttc ttccaacatg tcttttgcca gttccgagtt caataccgcg
     6301 ctaccgatat ccgcaaaaac aaaataacta gctgcatcgc ttgattcatt gactgcttct
     6361 acgattttca tcgggtctga tccgatctcg ccatttattc cgccgagaga aaaaatttgc
     6421 acctcttcgg atttttgcat ctgctcaatc atttctttaa taccatctgt tactttttga
     6481 ctatgagaaa taagcaaaat actttttttc acaagaatct tcctttcttt tacatagttg
     6541 ctaacgattg gtttcgcttt cattaatgaa caattcaagt atataataaa tttctaattt
     6601 tgggaaaata tgtgtagtgc ttcttgaagt ttgttatact ttaattaaaa gagaggagtg
     6661 atcgaatgat tacaacaaca acgaacagtg tagagggaaa agccgtagta gcttataaag
     6721 ggatcgtttt tggtgaggtc attactggga tccatgcatt taaagacttg gaggcggggt
     6781 tgcgaaatat ctttggcggc cgctcaaaaa gctatgaaaa cgaactaatg ggtgcaagag
     6841 aagaagcact tgccgaaatg gccgaacgtg caaaagcatt aggagctgat gcgatcattg
     6901 gcgtaaaaat ggactatgaa gtattaggca gcgacaatgg catgctgatg gtcacatgta
     6961 gtggaacagc tgtctccacg agataagtag gggacatctg ttttaataac taaagagaga
     7021 tgggacatgt agtggcactc aagaattaga tttttcagtc taacttcgag gtactctacc
     7081 ttcccatcct ctctttttta tttctttttg cggatgattc cgtaagctgc tgcgactaac
     7141 acacccgccc ctgctaagga ataactgaca gaagctgttt tttcatttgt ttttggcaaa
     7201 gtctgacttg ttgatttgga tggctctgtc gtttgctgaa ctgtttctgt cgtgcttcca
     7261 gaggtcactg atttttctcc tgccgaatgc aaaatatctt ttattcgttg gaaactagct
     7321 gctaagtcag ctttttttgc ttcaaaatca ttagcaactg tttgtgcttc gctgatcttt
     7381 ccgtggattg cttgactggc cgctgtgata tccgcttgag tttgacttag ctttccagaa
     7441 gctgtttgag caacctgatt taattgctga ttgaattctt ttgcttgttg gatcttcccg
     7501 tcaagatctg tttggacagc ttccgctttt gctttcgctt cggcaagctt tttctcaagt
     7561 tgtgcacggt catttcccac tttattttta agatcagcta aatcttgatc gattgctgct
     7621 tggtcttctt tcaatttgtt ttttgcagct tctaatgctt cttcgagttc ttttctttta
     7681 ttttccaagt cttgcaaatg atcgatttgg tcagaagagg tagaactatt tattgatgta
     7741 tcttctgaag atgaaatcgt ttctcctgaa ttatctgtcg gtacattgga agtcatttct
     7801 gtttccgagg caccgttttc agcaccagaa gagcttatgg ttgttgaagg ttctgtttga
     7861 gcaggttcag aagtttccgc aaaagctgtc tgtcccaata agaaaatact ggaaacagca
     7921 attggccaga ttaacggctt cattcttttt ttcatcactt ttcccatcct tactactttt
     7981 taactaattt cttattataa gcaaaaatgt ttggaagaac cataaacaca acaggctgtt
     8041 tgtctaaaaa acggtgcatc ttttattttt ttgtacgttc cgggcttttc catgtcccgc
     8101 ttccataacc taaaatcgtt ttgactgctg gaatgaaagt cgtcacgatc gttatcgcgt
     8161 tcactatcca gaacaaaagc atatatagtg gtgcgaaaag aaggtatttc aattttcttc
     8221 gtcgatcatc tacaatcagc gaagctagca actggaaaaa tcctgcgacc atttcaaagc
     8281 agatgaacac aaaactgaat gtaaaagtaa tataaatctg ttcgtagttt ccttgatata
     8341 cataatgccc gattaatcca gcaaaaagaa ctacggaaat acaaaagaat attgaccaga
     8401 caatactcaa tgtttgatca atgaagatga tcgttcttcc aatatgttta aaaggatgga
     8461 gcatcacttt tttgaaattc gtcagccaga cttctgttcc accttttgcc caacgttttc
     8521 tgtgtcgata tagcatcttc aacgtcacag gtacttccat gaaaaagata atctcaggag
     8581 caaaaacaga taaccagtca tttaactgat gatcccaagc aatactgatg tcttctgtcg
     8641 ctcgatcttg tctgaatccg cccacatcga tcaaggcttc ttttcgatac atcgtatttg
     8701 ctccactgta cgcataaaga ccaccgaaaa cagctgactg tgttcgttta atgatgccca
     8761 cgatacttga gaattcaacc gtttgagatt ttgcaatcaa tttggtacga ttttggacat
     8821 ccatatttgc agtgaccgca gcaatatgtc ttgcaccagg gcgaatgaaa taattcacat
     8881 acctaatcag tgcatctggc tccggtaccg tgtctgcatc attgcttaaa atcaatttcc
     8941 cttttgcaaa ggcaagtccg atattaaacg cgtgggcttt tcctttattt ttttcgatcc
     9001 gaaccacacg aagattagcg tattttttca tcagtctagc taatatttct ggtgtttggt
     9061 ccgttgaacc atcgtctgtc actaatacct catagttgtg ataattcaat ttcgtcatca
     9121 aatactcgat tgtatattcg atcacaattt cttcattatg ggctggcacc atgatcgtga
     9181 tgaacggttc gacactcaag ggaacatcta cccattcttt ttgcttatgt ttgtataaaa
     9241 acacataaca ccagacacca ataaaccata caaatccgcc taaaatcgga taagaaacga
     9301 gaaaaaaata cataatagac aaacaagtat ctaagaataa attacctgta tgggtgattg
     9361 agatcatttc agttccaccc cattttcttt atacaaatct gcaatgaatg tcgtatctag
     9421 atttttctct tcggagacag aataaaaacg gaccgtttct ctctcttctt ttgttccaaa
     9481 acgttccgta tacacttctt caagtaattc ttgtcttttt ttcaattttt catcttcata
     9541 ttcaaaagat ttatttactt ttttgtatag atttcgattg ttccacctag tcaatacaaa
     9601 tgaggtaatc aggaaactga aaaaaatcaa tacaaaaaag ccgattaaga aatacagcat
     9661 tttcttttct gataaaaaag cgtacaagga aaaatccttg gctctttctg gtctcgttaa
     9721 tgggaaaaag acccatataa aaggaagaac aacgatcatc catcctaaaa gagccactaa
     9781 cgtttgccaa atctttaacc agtaattacc ttttgaaaaa tacttatctt tcaccaaaat
     9841 tgatgcaact tcttctcttg ttactttttt catatcctca cctcacgttt ttttgttacg
     9901 acgtaggagt ttaccataca tttcatttct tttcacataa agtgttccta aatttttcct
     9961 aagaaaaaga acaaaacatt tctcatcagc aaatgaaaaa tattttgttc tttttatcag
    10021 ttgttctttt tattttttat ctcttaatta tgttgctctt tccaatacga caatgctttt
    10081 cattcctcta gatatcgccg tgtacatttg tctgcgtctt ctttgctcat ctccatcgtt
    10141 ttcaaatgga tataagatta catgatcaaa ttccagtcct tttgctagac tcaaaggaat
    10201 gatttggcag ttttctaatc catatgaatt ttttttaatc gattcttgta gcgtttgggt
    10261 ttcctcttct gtttgggtga taactgccgc tttttctcct tttagagaaa ccaaaatctc
    10321 agctagtatt ttcaacaatt cttcagagga tcgaaaagag cggtattcgg gttcttttcc
    10381 ttgcggtcgg atcgatacga tgtcgatctt ctcttgatcc acagcatagg ttttgaaaag
    10441 ctctgtgatt gctccgctag aacggtaact atttctaaga tcataccgat ggatcggcag
    10501 atggtagtcg tcgaaacaac gcataatatc agcaaaagta gtggaagaat taaagattgc
    10561 ctgattctcg tcacctacta aagtaaagtg agtttttgga aataattcga tcaatagacc
    10621 taattgtgcc agggtataat cttgtacttc atcaatcaat agatagcgaa atgctggtac
    10681 atttaccttt tcaacaagta aatgacgaat agctaatata atgactgctt catcaacagt
    10741 acgtgtgggt tgccaagcat aagctgagcc atatcgtttt tcataaattc tttcaaacaa
    10801 ctgatgctca tccacccatg ccatttcttc gatttgtctg gttatttttc gatatttttt
    10861 ttgtaaaagt ttccttgcat aagctacaat actttgttcc gaatcatcag aaatcaattt
    10921 accgaataat tccaactgcc tatcttctgt caacgagagt acctgattct gcacagcagg
    10981 actttttgct tgtttcaaaa gatgattctt ccattcgctc atcagtgcct ttttcgttgc
    11041 ttgaagccta tcgatcaatt taggatgagc aggaactttc tcatatattt tttcgatcgt
    11101 ttttttagaa aggactgttt ttccattttt agtaagtcca cggataaaat tcggatgatc
    11161 taaaaacaaa ggatctgatt gttttagatt atcgataaac tttttgctac gcaaacgttc
    11221 tgtctgctcc gatacatttt ctcccgtgat acgtttgaaa taagcctctt caccttctat
    11281 ttcctctgct gaaagctgtg aaaccagctg tatgatggtt agattcagtg gatttctttc
    11341 acctaatgat ggtaggacat tcgaaatata gtcgataaac cgatgattag gtgatagaat
    11401 cagcaagtca tcggatgtca tcgtatctcg atataaatag agcagatacg caatacgctg
    11461 catgatagca gaggttttac cacttcctgc tacgccattt accaagatat tttgactttt
    11521 cacatcccga atgattgtgt tttgttccga ttggatcgta gcagtgatag ccttcatttc
    11581 attggtctca tcttggccta agacttctaa taatacgtca tcttgaatag ctacagacgt
    11641 atcaaaaaac ttgaccaaac gatctctttc caaaagaaat tgtcgtcttt ctttaataac
    11701 cgtatcgatt ttattttgac gaaccgtata agaagatgga ccaatcatgt tattgtaaaa
    11761 aagttcggca attggtgaac gccaatcata aacaagattt tcatctgcat catctgtgaa
    11821 atttgctgta ccgatataaa aggattcttt tggctcatta tccagaaagt ctacaacgac
    11881 ttttccaaag tagggacttt tcaataaacg ttcgatcttt gctagctgat tttctgcgtt
    11941 tttctgtttg atattcatct gatcgatttc tctatttttc atttcaatca tcgaaaaagt
    12001 atccaggtta tctaagtaac tgtcaaaatt caatcgaagc tcgccagcca tttctttcaa
    12061 cgactggttt ccttctattt ttgccgtatc catcgattcc cgcagttcgt tttttttatc
    12121 aattagcttt tgatacacgt tcttcagata tgcttcttct aactgttttt gtgtgttgac
    12181 catacacatt ctcctctttt tttaaagtgg tggaaccgat tagtttacct cttttaagag
    12241 tcgaatgcaa gctttttaac gagtatcatt tgttaggata tatcagttct tcaggtcgga
    12301 cagtttgata taaagaaaat gcaccatcaa ggttttttac gtggtagcca gactgcttca
    12361 agattctttc cgctagatag ctacgtaatc cgctatgaca gctgacgata tattcttgtt
    12421 ggctgtctaa ttcatttaat cggtcccgaa gttcattcaa agggatatgt ttggcttcct
    12481 tgaatttgcc cttttccaat tcttctgctg ttcgcacatc taataaaatt tttccttttg
    12541 ctagttctgt cggcaattca tgccattgta cggtttcact aagcccttct gcaatgttca
    12601 ttgctgcata tcctagcatg tttactggat ctttggctga gccaaatggc ggtgcatacg
    12661 tcaattctaa ttccggtaaa tcgaagatcg tcaaatgccc tttgattgct gttgctaaaa
    12721 tatcgatccg tttatccaca ccttttgccc caacaccttg tgcaccataa atctcgccag
    12781 tctctggatg gaagatcaac ttcagtaaaa tatctgttgc ttctggataa tagcttgcat
    12841 gatcttttcc tgatacatga atgacttcaa aagacagttc gttttctttg gctgttcttt
    12901 cacttaaacc ggtggatgcg gcagtcattc caaacgttcg tataattgct gttccaatac
    12961 cgccttgatt tcttcgagaa attccggaaa tcgtgtccgc aacttgtcta ccttgacgat
    13021 tggctggtga agcaagagaa ataagtgcat cttttccagt gatttgctgt tttacaacga
    13081 tagcatcccc caccgcataa atatcaggaa tattcgtttg ataacgttcg tctactagga
    13141 tacccccgcg tatgcctaat tcgattccag cggtcactgc tagctggtta tcaggacgaa
    13201 caccaatgga taaaataact aaatcagcag ggatggtacg cccatcatcc agtcgcaatc
    13261 gtttcccgtg gtcttcaaat ccagcgacag catgagaagt aatcacttct actccttgat
    13321 gagacaattc tttttctata aaagctgcca tttcttcatc cagtgttgga agaatatgtg
    13381 gtgcttgttc gatcaccatg acttctaaac ctcttctttt taggttttct gccatttcca
    13441 atccgatgaa tcctgcacca acgatcacgg cacgctttgg ctgtttttct aatgcatgca
    13501 tcactttatc gatatctggt acatttctaa tagaaaaaac attgtcggct tctgttatcc
    13561 ctggaatcga tggaacaact ggttttgcac caggagataa aaccaatgca tcgtactgtt
    13621 caatgtgttt ttgaccctca tgtacgactt ctaccgtttt gttttcggga aagatggctg
    13681 tcacttcatg atttggacgc acatctaaac aaaaacgagc ggataatgat tctggcgttt
    13741 ggacaagaag attttcccgt tcactgattt ctcccgaaag ataataaggc aagccgcaat
    13801 ttgcaaaaga aacatacggt cctttttcaa aaacaacgat ttctgcatct tccattaatc
    13861 ggcgaagccg tgtagcagca gacattccac ctgctacacc tccgacaata acgattttca
    13921 tcttatttcc ctcctactgt tcgatcaaac cattgattca tgccaccacg tacattcact
    13981 acatcataac cagatttctt taattcttta gctgcttgtt tactcctcat ccctgattga
    14041 caaatcacat agacggtctt ttctttgtct ccatcatatc tgtttatact ttgtaatgga
    14101 acatttttag ctccttttat atgcccgccc cggtattctg caggtgttct aacatcaagt
    14161 aacgctggat tttccaaaag cttttccttt aattctggga ctgttatcga ttttattgat
    14221 tgaaatagca aattcatttt ctgcacctca tttacatttt tataaatacc tcatggggta
    14281 tattaaacag gagctttatt aatatgtcaa ccagaatgac tcttcatttt tttcactact
    14341 ttttatattt gcataaaata ccgataaggg gtattatcta attataaaaa tagaggagaa
    14401 tgtgttatgg agattgataa taaaaatatc ctgaaccgtt tgaaacgtac agagggacaa
    14461 attcgtggta tccaaaaaat gatcgaagac gaaaaagaat gtatggatgt catcactcaa
    14521 ttaagcgctg ttcgttctag tattgatcgc gtaatgggca tgatcgttgc agataatctg
    14581 aaaaattgct ttgagaaacc tgatagtaat ccagaagaac aagcggctaa actagaacaa
    14641 gcgatcaaga tgattattaa aaagtaaagg aatgatttca atgaaagtcg ttttccatat
    14701 tgatgaatta gaaaaatggt cagaaacagg gaaaaacgta aaaaacctga taaaagcttc
    14761 tccagaaacg acgattgtcg tatctgtaaa tggcatcgcg attaccggtt atctagactc
    14821 tgccaatgca gaattcttag atttgcaagg agtcgttttt cacgcttgcg ccaatgctat
    14881 gcgtgctaat catatttcag aaagttcttt acctgaacaa gtaatcgttg taccagcagg
    14941 tgtccttgat ttagttgaac tgcaatccca aggttatgct tatatcaagc cttagtcagt
    15001 agaggttgcg acagaagtga tcagcttcaa gaaatttcgg cttatttttc gaagaagcta
    15061 cttctggaaa accgtgaatc aaaggttgtg aaaacgctgt cctgcatcaa gtaataagaa
    15121 cagccaaaca aaaatagctt ctcatatttt tcgtttggtt gggcttatta ccgcagatgc
    15181 aagcttttga acaccgttta aggaagaggt tgtgaaatca gcggtttgac ctaagtatta
    15241 gagaaaaaaa cgggaaaata gctcctcata ttttgccgtt ttttgagcta ataccgaagg
    15301 tcagctgatt gaacaccgtt taaggaagag attgtgaaaa agtcgcttag tttcgagtat
    15361 taagaaaaat tttgtgaaaa tggatttcta catttttgca taaatttggc ttaataccga
    15421 gaaactggct tttgaatacc gtttaaggaa gaggttgtaa aatcagcggt ttgacctgag
    15481 tattagagaa acaaacggaa aaatagctgc tcatattttg ccgttttttg agctaatacc
    15541 gaaggtcagc tgattgaaca ccgtttacca agaggttgtg agcctaccat ttaactccga
    15601 atcacaagaa ataatttcaa aaacaacttc tcctgttttt ggttcggttg gcttattatc
    15661 gagaagtagc tactgggagt ctatttatca aaaaaaggaa tgcgacatag gtcacacccc
    15721 ttttttattt tctttgaaac atctttcggt actcgacagc atacaagaca aaagattgta
    15781 aaagaccgta agtgatgacg gcaaaaatga ctctcaccca cagcgttggt aattctggga
    15841 aaagcagcaa aaggatcatg ccaacataga tgacagcagt cgctactttg ccataccact
    15901 tcgctccact taatttgata tttttagata gcccgattaa tccagctaca aacataaagc
    15961 cttcttttat caagaataga ccaaacaaca gccaaagcca cggtcgatac catgccatag
    16021 acaaaatcag aaaaagttgt gtcaatttat ctgcaaacgg atcaagcacc ttccctaagt
    16081 ctgttacctg attatatctc cgagcaatga ccccgtctag ataatcagac aaacctgaga
    16141 caacaatgac cgctcctgct atataaaatg actgtctcag cacaaaataa agataaatcg
    16201 gcagcaaaaa aatacggaat atcgataaaa gattcggaat cgtttgtatt tctttacgcc
    16261 agtctgtgct taatttcatt ttatcgccct acctcctcag cactcttttc tttttatttt
    16321 agcttacacg gggaataaaa ccaatgattt cactgttttt atttttcttt acagacactg
    16381 atattatgag ctaatttgag ataatcactt gctgttttca tcgtttactc ttctttcttc
    16441 ctgtcgtttg cgcatttctt ttactagtac tttatgagct ttttttgatc ctcgctctag
    16501 gtcacgctgc cgtttacgtg ttttataatc gataaacaga ctgtctaaat cgtaacctgt
    16561 cgggtacaga tcttctgctg aacgcaccag acgaacacgc tgacgaggca cgtggattat
    16621 ttttttgtct aaatatacat caatcgtatc ttcacctata tcttcataaa ctaacgcttc
    16681 tttttggtat tcattgacaa atatccgatc accttttgca aagagctgac gttctttttt
    16741 attctccttc ctatttgtaa ctgctgtctt aggaagaaat tcttttttct gtgtccgata
    16801 ggctttttcc gccaagtaac gctctgcttg atggatcaat ttcatcgaca tatccatttt
    16861 atgagcaatc cacaaagctt ggctttctcc tgtttcttcc aacccgcaac tgatatttag
    16921 gacgaagtgc ttcccgatcg aaagccatag cagccggcac aaaatcttca tgatctccgg
    16981 cgaatttctt gatttctcca taatgggtcg tggcaatgat caatgcccct tgctcataca
    17041 tagattccat gattgcgatc gctagtgcag ccccttcatt tggctctgtt ccactgccaa
    17101 tctcatctaa aagtaccaac gtattccttt ttatcttttt caaaatagca gcgatgtttt
    17161 gcatatgacc agaaaatgta ctcaacgcat tttctaaatt ttggtggtca ccgacatcga
    17221 cgaaaatctc atcaaagaca gcaagctctg tcccttcttt tgccggaact tgcataccga
    17281 acatcgccat caaagtcaat aaaccgactg tcttcaacac gactgttttg cctccggcat
    17341 ttgctccggt gataatcaag cctcgatagt ctttaccgat ttcaacatcc aatggtaccg
    17401 catgctcagg taaaaatgga tgtctgcctt gcttgataat gatatgttcc gatttattga
    17461 ttctaggtgt gcttccattg atttctcggc taaattttgc ccgagcaaag atgatatcta
    17521 acaccgtgat cgtttcgatc aataaatcga tcacttcttc gttttctgcg atagcaccag
    17581 tcaatgccgc caggacttga tattcttcac tgatttcttc agcttttaac aactgatagt
    17641 gttcattcaa tttagaaatc acagttggtt caataaaaac cgtcgttctt ttatttgatt
    17701 catcgataat cgttccatct attttatttt tatagctggc tttgatagga atcgtatagt
    17761 attcgccttt ttgaacaatc attgcttctt ggatcatttc tttgtttttc ggatgacgca
    17821 agaatttcag taattttgac tggatttctt tttcgattag ttgtaattgt ttgcgtactt
    17881 tacgcaaatt acgtgacgcg tcgtcactga ctttattatt ttttattttc tggtcaatca
    17941 actcttctac atttatcaaa tcaggaagat gtttgctata agcaaacaac aagggtgtct
    18001 ggtattgatt cttatcaaaa aactttgtga tcatccgact gcttctaaga aaatccgcat
    18061 attcgatcaa atcggttggc tgcagaatca accctttttt gacttgttct gtcagtgcat
    18121 cgatcctcgg caaccccata aatggaacat gctgactgct ttctaagatc aagcgtgctt
    18181 ctcttgtttc ttcttgacga tccaagacag tttgtaggtt tgtcgagacc gtcatatcgc
    18241 tgattcgttt tttgctatag tttccaattg ctcttgcttg gatttccttt ttgatccgat
    18301 caaattgtaa ttgactaatc atttcgttgt ccatattctc tccttttctt gaagagatac
    18361 ctgtcccaaa ataaataagg gcctagagat aaataaaagg atagaacaca gcttaaaaaa
    18421 gtcatgtcct atccttatta gaatacaaag atattgtttt cctgacaggt accgtttcat
    18481 aagcacacca aataaacttt gtttattggc tattcgactt actgattaga taccagctac
    18541 actcacaaaa cataccccat tcttctaggt cttatttatt ttccacttaa ctatagcaag
    18601 acacattttg gttgtcaaac tttagtatgt tttatagatt tacaccatta atgaatagac
    18661 acctaccgcg ataattgctc cgatcattgg acctgcgatt ggtacccatg aatatgccca
    18721 atctgaagtt cctttattag ctattggcaa taccgcatgt gctaatcgag gaccaaagtc
    18781 ccgagcaggg ttgattgcat atcctgttgg ccctcctaat gaaagaccga tggctaagat
    18841 caatccacct acagcgaata cgttcgttcc aggtgcaaat tcgttttgtc caaaagctag
    18901 caacccaaga actaatacaa atgtaccaat taattctgtg atcacatttg ctggataatt
    18961 ccgtatagca ggacctgttg caaacgttcc taaaatcgcg ctttcatctt tcgttgcatt
    19021 ccaatgtggc aaataagaaa gccaaacaag aatggcccct gcaaaagctc ctaaaacttg
    19081 cgcaatgatg aaaggaacga caagattcca actaattgcc cctgtcatcg ccatagctag
    19141 agagactgca ggattcaaat gagctgggcc catgaatcca gaaatataga cagccattgt
    19201 tacagcgagt ccccaaccaa atgcgatgac aatccagcca gaagcctgtg ctttactttt
    19261 gtttaaattc acagccgcac acacaccatc tccaagcaaa actaggatca gtgtcccaat
    19321 aaactccccc atgatttgag tcatatcact gttcatttat ttctccccct ttagttcccg
    19381 cagatctgat tctgcaatca atgcttctaa acgtttagtc tgttgcgcct tctcctcttc
    19441 tgtccattca aaatatccag caattgcatc gatcacaggt tgcttgatct catcaagctg
    19501 atctcgttca aacaacagat gatttgtacg acgaatgaga taatcgcctg gggccaaaac
    19561 catttcttct tctaaaccat aacgtaaccg agctgactca gcgagactca aaccaggata
    19621 aggtgccatt tcttttgcta attcaaagat acgtcgggca ttagtcccgt aaaaatcagc
    19681 gatataagta gcatcttctt cctctaaacc ggctgctact ccttcttttg ctaattctgt
    19741 cactgtttct tctaatttag ttggatcgaa gtttccacct gaaatctgat attttttaga
    19801 gtcgatttct ttcgtctcta ttccgtattc ttcttttaat aattggcgta tcaatcgcaa
    19861 agctccttct gccatcttac ggtaatcggt gattttccca ccagataaag tcagcaaacc
    19921 atctggttct ctttctaaag aactacctct ggaaatcgta gaaggtgctt tttcatcacg
    19981 gctgttttcc aaatggttca acacgtcttc tacttcagca cgagaaactt tgttttcctt
    20041 atattcactt actgtatcga caactttatt gaagctcttg tctgaaatcg atccattatc
    20101 tccaccattg taatcagaac cagaattacc aatcaacagt ggacgaagtc ctgcccagct
    20161 tgcttcgata tcattcaatg tgatatttgc ttctggatag cgatgattga tcacatccaa
    20221 tagataatct acatcttctt gtgtgacttt cggatccgta aaatctcctt ggtaatccgt
    20281 atctgtcgta ccaaagtaag tcttgttttc tcttggaata gcaaaaacca tccgcccatc
    20341 ttgctttcct gtatcgaagt atgtgggttg cggtacaggc agttttttcg catctacaac
    20401 taaatggatc cctttagttg gacgcatttt aggagagact ggacgcgtaa aattcaagtt
    20461 tcgtacttta tctacccaag gaccactcgt attgatcact aattttgctt tgatttcgat
    20521 cacttcatct gtcagcaggt cacgagcttt gacgccaacg atttgatcgc catcatataa
    20581 aaagccaacc gctttcattt tacttactag ataagcccca tcttcggcag cctttttgat
    20641 attatcaatc actaaacggg catcattgtt gcggaaatct agatagacac ccgcgccttt
    20701 tagcccttct tttttcaaaa acggttctct ttccaatact tcttctggtg tcagcgtata
    20761 attctcatat ttggttcctg tcacatttgc cagtttgtca taaaggtcca ttgctacttt
    20821 gaccgagaac atattgaagg ttgttgctcc ttcgccctca tagattggca aaagcattgg
    20881 atctggtttt gggatgtgtg gggcaatact ttgtacgact gcacgttcac caactgtgtc
    20941 agctactact tccacatcaa atgtcttcag ataacgaata ccgccatgaa caagtttggt
    21001 cgagcgagag gaagtccctt ctgcaaaatc ctgcatttcg atcaatcctg ttttgattcc
    21061 tgatgctgct gactggactg ccacaccggc accagtgatt ccgccaccga tgatcaacag
    21121 atccaattct tcttgctgca ttttttgaat gctatcttgt cttgtcttgt ttgaaaacat
    21181 atggatttcc tccttattca aatgcttggg tagcttttac tgcttttttc caaccgttgt
    21241 aaagtttttc tcggcgctct tcctccattt gaacttcaaa tagcttgcca gcttcataaa
    21301 attcgcggat ctcgtctgta tctttccaat aaccgacagc taatcctgct aaaaatgctg
    21361 cccctagcgc cgttgtttcc aagttttctg ctcgttggat cggtacattc aagatatctg
    21421 tttggaattg catcagatat tcattgtttg ctgcaccgcc atctactttc aatacaggaa
    21481 tcttgattcc agtgtcatct tgcatcgtat cgatgatatc tctcacttga taagcaatcg
    21541 actgtaaagt agctttaatg atatcttctt ttgtcgtacc acgtgtcaag ccaaacatag
    21601 cccctcgtgc tgcctgatcc caataaggag cacctagccc aacgaatgca ggaacaacat
    21661 aaacctcatc ttcgcttgta gagcgtttcg ctgcgtcttc tgaatcgcta gctttttgca
    21721 acatttgaag tccatctctc agccattgaa tcgaagaacc agccacaaaa atacttcctt
    21781 ctaaagcata gtaaaccttt ccatttatgc cgtaaccaat tgtcgtcaat aaattatttt
    21841 tggataattg cggtttttca ccggtattca tgacaataaa tgaacctgta ccataagtat
    21901 tcttgaccat gcctggttcg aatgccattt gtccgaataa tgcggcttgc tggtctcctg
    21961 ccatccctga aattggtgct tcgcttccat aaaaatgata accttgcgta taaccataaa
    22021 cttctgagtt agaagtgact tttggtaaca tcactcgagg aatatttagt aaatctaaaa
    22081 tctcctgatc ccaatctagc tgatggatat tgaacaacat cgtccgactg gcattcgagt
    22141 aatcagtaac atgtgcttgt ccatcggtca gtttccatac taaccaagaa tcgatcgttc
    22201 caaacatcaa ttcgcctttt tcagctcgtt cttgcgcgcc ttctacatga tcaagaatcc
    22261 agcgtatctt cgttgctgag aaataggcat cgatcactaa ccctgttttt tcgtggaaaa
    22321 attcttgata gccttcattt tttaagttat ctgcaattcc agctgactgt ctggattgcc
    22381 aaacaactgc atgatagatt ggacgtcctg tatctttttc ccatattact gttgtttcac
    22441 gctggttcgt tatgccaatc ccagcaatct gatttggttt gatccctgat tcgatgaacg
    22501 ctccggcaat aactgactgt acagaattcc agatttcatt tgcatcatgt tccacccaac
    22561 tttcttgcgg gaaatattgc gtgaactctt tttgagaact gccgatgtgt cttcctttct
    22621 tgtcaaagat aattgctcgt gagctagttg taccttggtc aatcgccatg atatacgttg
    22681 attctgtcat tgtgattccc tccattatat gcaaacgctt tctttaacta ctcttatcat
    22741 actactgttt taaaaaaaga aactgtcaaa attttattct ttttttcttt ttttgacagt
    22801 tttttattat gaagctattt tttaatcaat tcttttcttt tttcagcgtc tctattcttc
    22861 ttttcaatcg aataatgtcg taaggtgacg caaattctga cagataatat tcacactgtt
    22921 ttttgtttct ctggcaaaat gtgatcacca aatcatattc tttaccttct tcataacttc
    22981 caatctcaat accgccgatt cccctcagtt ctctgatcag atattgttga aaagcaatac
    23041 gataaatcgg cagactttct aaatcgattc caatcactac tggttttgca atataaaaat
    23101 cgatcatcaa aagaacattg gcatactgga tcatcaggta actaagctca ctatcttcag
    23161 ctcttttcct cggcaattgc tgcaccgctg tttcctgtaa tttcgccagt aactgtgtta
    23221 aggaatgccc gagcattttc tgctgctgtt ccaaaagccg atcacgctca tagacttcga
    23281 tctttccaac aaagaaaaac cattcattgt tgatctggga aatttggtag cctactgcct
    23341 tttcttcttt gattttcaaa cgacgaggac gataatgcca taacatcgtt tcccgaatgt
    23401 aagtatctaa aacagcggtt ggtaactttt tgctccgtgt caaatcatac tgataaaata
    23461 tttcttcgtc tactatcgaa aaagaaacaa aaaagctgta aaacatcatt gtttcgtata
    23521 cattcagttc tgctgctgtt cgactaagat gcatcacgat gattggatcg attgcttgat
    23581 aaagccggtc cgactgatag agtaatttct tttctttcaa agttgcgtac gtactttttt
    23641 cattcaatag tctttttttc gtaatcccga gccagcaagc aatctgtgct tctgctgatg
    23701 ccgtaaacgt tgtttctaat acacggtcca atcctagaat gaacggtctt ttttctggtg
    23761 tattttgcaa aaaaagcggt cgctgatctt ccgggatata ttggaatagt tcataataaa
    23821 aatatcgtat ctgtagctct tctccgtata attgaccgtt tttgatttgt aatccaaatt
    23881 cctctaagag ctggttcaat tctttgatct ttcgaaaaac agaagactca ctgatattaa
    23941 acgctgtcgc taatcgaacg atggatactt gctgatgttc taataaatag acgagcatct
    24001 taaacttcaa gctttcctgc aataaaaaca atagaatttt ttccaaactt acatcttcgt
    24061 ccaacacaag cagcgcctga tactcatttc gctgtacttc tacttttttt tccatcattc
    24121 ggccaatcaa ttcaatatct tccaaatatt gatcaaacga ggcttttgac agttggacat
    24181 gctctctcaa accattgata gaggctgttc ccccttgcag aacaaggttt cgcaaaatca
    24241 ggatctgtaa ctgctctttt ctttctaata atgcttcaat cttcataggc agtattcgcc
    24301 acctttgacc acaccatatc tggcacacgg tattctggct ggaaaaatag ctcatgttct
    24361 tctaactgtt caaacgtcca tccttcttct actgcaattg tcagttggtc aagaaggtgg
    24421 aacatctctt ttttgctttg gagctgtgct ccaagaatct ttttttcaga atcaaataga
    24481 aatttgagtt taactttgct ttgatttatt gctgtaagct tgtagtctac gtatcctgtt
    24541 tcaatttttt ggggataaaa aatgctctct tcttctgtca aacctacgct tcctaagaaa
    24601 tagccaaacc actgattgcc gactggacga taggttccct gatcctttgt ctgtgagcca
    24661 ctaattgtct tgctgacatt tcgtgctgta cggacagcgt tatttactag agaaacatac
    24721 attttttcat ttgttggacg aaaagttact tggatcgcat cgccaatcgc ataaacgtct
    24781 ttttgagacg tttgcaagta gtcatctacc cagatcgttc catcatcatt caatgttaat
    24841 tgttcttccc acatctgatt gtcgggacga gaatgattag ccagcaaaac aagatcatag
    24901 ttctcctttc ttttttccgt ctctacaata gcggtttcat tttcggtcaa agacacaact
    24961 tgttcttcta aaaaaagtga aagcccttga tttttcattt cctttactaa cggttctgtc
    25021 atttcaggat ctagataacg aaaaagaatt gactttcggc tttcgtaaag atggatttgt
    25081 ttttttcttg atgccaaccc ttcagctatt tcgattcctg cttgtcctgc accaataatt
    25141 gcaattttct ttgcattcat gatcttttgc tgaagagccg ttacgttttg tatcttttta
    25201 actgggtgaa taagcgttga ttctgcagaa atatttcgaa acttttgatt tgaaccagtc
    25261 gctagaatca aacgatcaaa atccaatttt tttcctcctg ctaaaacgac ttttttttct
    25321 ttcaatccta ttactgtctt ttctgtgtaa acatggattc gatagcggtc agtcagttct
    25381 tctttcgtga tccaagaaag ttgtttttca ctggtaacct ttccttttaa tatcaaattg
    25441 aacccgctgg gaatccatcc taacttcgat tgcttttcaa tcaaatatat ttcacttgtt
    25501 ggcatcaatt ggcgaagata aatagccgca tgaatccctg caaacgaccc gccgacaatt
    25561 acaatgttca tctgcattcc tctttcttat aaggtgttct actgctaact tctcaatcag
    25621 aaaacacagt attcataact cgctttcaac cttatgatac atagcgatgc agcagaatca
    25681 accctctcca tcataccgtg ttctaggtct gcttctcaat aatgtactag tggacctaat
    25741 tgaagagtat catttctgaa gagatgtttc aaaaaatgta ctcttataaa agaaaaaaac
    25801 tcgcctttta cgtacctctg tccaaatagg aaacagaaaa gaacgaaaag acgagtttta
    25861 gcgtgtgtaa tgtaatagtt agaatagaca catcatttat ttttaattct ttaatgcttc
    25921 aacaactgct tcgcctaatt gatgagcaga ttgaggattt tgccctgtaa gaagccgccc
    25981 atcaatttga accacttctg aaaaagctgc tccagactga tagtttgctc ctttttcttt
    26041 caaagcatct tctgctaaga atggtacttc attcgttgta ccatttgctt cctcttcttc
    26101 attactgaac ccagtcactg tacgtccagc taaaatagat tttccagact catctgtaac
    26161 tggcaacaat cctacaacac cgtgacaaac agctgagacc agcccttcat ttcgatagat
    26221 ttgtgaagcg actttcccaa gatctattgc ttctggaaaa tcccacattg ttccgtggcc
    26281 accagcataa taaaccgctg cataatcatt tgcatccaca tcatcaggac gtaaggtatt
    26341 tcccaaggcc cgatttctga aatcttcatc ttcataatag gcccaatcga tttcatccat
    26401 attcaaaaca ctttgcggtt ctaaaggtac atatccgcct ttaggactga caaaatctgc
    26461 atcgatccca ttttttacta atacatcata aaaatgagtc agttcactta accacaaacc
    26521 tgtaggacgc tcgatctttt cgtacttttc tacatttgtt aatacgatca atactttcat
    26581 tttcccatcc tctttctata aaaattgcgt tattgataac gtgctttcaa gaaattatat
    26641 aatgggcttg gcaaataaag ctctttatat gcttcttcaa aaggtttcgt tacaaattca
    26701 tcctcacggt tttccaataa tttctctgcc cagtcaggat cgacgatcat ttgctgtcca
    26761 attgctgcaa gttctgcata tttcaatacc tgctctacat cttcgcgatt gcggatccca
    26821 ccgacaccaa ctaaagggat ctttccttga atcgtgttat ggaccacttc aagaatcgat
    26881 ttatcgttgt atttatcaga acgagctatt cgatcataca tatttaatga aacatgcaga
    26941 taatctagtt ttgactcacg taatcgttca agaagccaga tcgtgtcttc aaaacggata
    27001 cctggtgttt catattcttc tggtgagaaa cgatacccta cgatgaatgg ttttttagca
    27061 tattgttcaa ccgctgagaa aaccgttgtt aataattctt caataaaacg ataacgattt
    27121 tccaagctac cgccccattg atcctctcgt ctattcgaat gcggagagaa aaattgttgg
    27181 atcaaatacg tatttgctcc atgcaactcg attccatcaa atcctgattc aatcgctcta
    27241 cgtgtggctt tgccaaatgc ttggattgtt tcaacgattt cttcttcact cattgctcgt
    27301 ggtgtttccg catcaggacg ttttgctgca actgcacttg cagatacagg ctgttctcca
    27361 gataaagttg ctcgacttgt cattcgtcca ccgtggaaaa tctgaataat cgcttttgcg
    27421 ccttctcctt ggattgcttt cgctagctca cttaatcgag gaatcatttc atcatgagcg
    27481 atgctgagtt cacctggcca gccttttccg atatcttgga catttgcagc accagtaata
    27541 acagcaccaa gacctttaga acgttgttta taataagtga tctcatcttc ggtgatcact
    27601 ccattgaaaa agctgagttg tgtcgtcata ggcgacagca tcaatcggtt cttcaattga
    27661 actcctgaag gtaaagtaat tgattgatca aattgtgaca ttgtcattcc tcccataatt
    27721 cgctctgatt aacctatcga gaatcatcta tcattaaacc actcgattag ttgtacatgc
    27781 aactaatttg cttaaaaaat tttatttttt ttgtaactta tctgttgcat cattcattgt
    27841 ctgggctaat tctttacttg cttcatttcc caaaactgct ttgtatgact gatgaagttc
    27901 ttcccagcta ccatcgactt ccttagcaaa ttggcgtcct tgttctgtaa gataaactaa
    27961 tgacaagcga ccttcgctaa ctgttttgac gtatccttta tattctaatt tttcaacaaa
    28021 acgagcaatc gttgatgggg cggaatagag catttgtgca atatcttttt gtggtattcc
    28081 atctttttct tttaatatca ataaaataaa accataagtt ggtgccagac ctgtccttct
    28141 aaacgcttca tcagctaatt tattcaacat gcgatctagc tttttcacgc taaagaaaag
    28201 acattcatct aacgcttttt caattggctt ttccatttca tcccctccat tggcgtacat
    28261 atcatagtat gatttttatt ttttgtcaaa aaacacggaa taatgtccgc ctcctctgtc
    28321 tagatttgaa aaaaagaagt agccatcttc aaagaatttc ggcttgtttt cgacaaaact
    28381 ccttctggaa caccgtgaat cagaggttgt gaaaaagtcg tttagttttg agtatgaaga
    28441 aaaattttgt gaaaatggct ttccacattt ttgcataaat ttggcttaat accgaaaaac
    28501 tggcttttga acaccgttta tatcagaggt tgtgaaaaag ccgtcctgca tcaagtaata
    28561 agaacagcca aacaaaaata gttcctcata ttttgtcgtt ttttgagcta atgccgatgg
    28621 tcagctgatt gtacaccgtt tattgagagg tcgtgagcct gccgttgaac tccgagtcac
    28681 aaggagcaat ttcaaaaaac agcttctcat gttttatgac tatggtgact tgtgatcgag
    28741 gagttggcag gagaatactg tttattcgta aagaaggggc tgtgacaaga cttttgtcac
    28801 agtcccttat ttccttttat tataggattt cgccatttga ttggatgact tgtctgtacc
    28861 aatcaaatga ttttttcttt aaccgtttct ttgtgccatt cccttcgtca tcttgatcaa
    28921 catagatata tccataacgt tttgacattt gtgaagtaga agcgctgacg atatcaatgc
    28981 agccccacga cgtataacca aataaatcta ctccatcttt gatagcttcc ttcatctgct
    29041 caatatgctg cttcaaatag tcaatgcggt aatcgtcttg aacggttcca tcttcttcta
    29101 ctttgtctat agctccgagt ccgttttcag ctatgaacag tggtttttga tacctatcat
    29161 ataattgatt caaagcaatt ctcaaaccct taggatcaat ctgccagccc cattcacttg
    29221 atggaagata cggattttta attcctccag ttattaagtt tccttggacc ccttcccaat
    29281 tttctggatg agcactgact gcactagaca tgtagtagct aaaagaaaca aaatcaacag
    29341 ggtaggctgc taagatttct tcatcaccta cttctatttt taagttgaca cctttttctt
    29401 caaacattcg ttgcgtatac gctggataat atcctcttgc ttgaacatca ctaaagaaca
    29461 aagaagttct ttgctgctca attgtttttt gaatatcatc cggatcacaa gaatacggaa
    29521 caagaagctg atagctgatc atacaaccga cttgtgaacc tttgataatt tcatgacaag
    29581 cttttgtagc taaagcactt gctataaact gatggtgtgc accttggtat ttcgcttgtt
    29641 ccaaatgggg atggttttct tcaattactc ctacgctaac aaaagggtga tgttttacac
    29701 agttgatctc attgaaagtc atccaatatt ttactttatt tttatagcgt ttgaatactg
    29761 tttcagcgaa acgaacgtaa aactgaataa gctttcgatt aggccatcca ccatattctg
    29821 taaccaaata taacggcatt tcataatggg aaagagaaac gactggctca atcccatatt
    29881 ttagacattc gtcaaacact tgatcataaa aaaataatcc cgcttcattg ggtgtttctt
    29941 catcaccatg aggaaaaatt cttgtccatg cgatagacat ccgatagcat ttaaaaccca
    30001 tttcagcaaa taaacggatg tcttctttaa agtgatgata gaaatcattt ccgtgtcttt
    30061 taggataata atacttgctg ttgggatcct gcgccgcttt tacttgctga tgcgtcatca
    30121 tatgccattg atctgtccat ttttctttag gtaaatcgct gtcgaatgta tacacatcag
    30181 acacagataa tcctttttca tctacatccc atgcaccttc acattgattg gccgctgtcg
    30241 ctccgcccca taaaaatcct tctggaaatt ttttcatatc gatcccccta aacttgctag
    30301 ttttcttgtt tgattatttt tcttatgact agtaatgata tttttgaatc tactactatt
    30361 tgtaacaata accattgtgg tcggatcata tccctctttg attaattctt gcaaatctac
    30421 ttccatcaat aaatcgcctt tacgtacttt ttttccatca gatactgcac tttgaaagta
    30481 tttgccattt aaattcactg tatcaatacc gatatggatc agtatctctg ctccttcttc
    30541 actgactacc ccaattgcat gtttcgttgg aaaaactgtt gaaacttccc cgtttacaga
    30601 actgtagatt tttccgtccg ttggaatgat cgcaaaccct tctcctaaag atccactagc
    30661 aaatgttgga tcgtttactt cagataaatc aattatttca cctgctgcag gagtacttaa
    30721 cactatatcc tttgaggatt cttcttcgat ttcatccttg tagccgaaaa gaaaagttag
    30781 catacaggaa ctgatcatcg ctaagaaata actagcaact agccaaacaa atccttcacc
    30841 aatataaatc gggaaagaca aaatacttct tgtagcaact gccaaagcac cggctcctgc
    30901 ggcaccagct actccaccaa atatagctcc acatatacaa gcaatgtaaa atggtcgttt
    30961 atatttaaga gtcacaccat aaacagcagg ttcagtgatt ccaaataatg ctgtgataaa
    31021 tgcagataaa gataaacttt ttactttctt atttttcgtt ttcaaaaata ccccaagtac
    31081 tgctcctgct tgcgcagcta cggcagtcat acatgctgga cctagcgtat cacgaccgta
    31141 gaaagaaaga ttgttggtca ttactggtac aagtccccaa tgtaacccaa aaatcactaa
    31201 cacttgccaa aatccgccga taacagcacc agataaaaca gggctaaagt tataaacagc
    31261 taaatatcca gctgcaatca gctgacttgc ataatctgca attggaccta caactaataa
    31321 aataattggg accatcacga tgatacaaat gaatggtgtc aataaatttc gaatactttc
    31381 gtgcaagctt ttgcgaatct taggctcaat ataagaaaga acccatacag ctaaaattgc
    31441 tggaataacc gtacttgtat accttgctag aatcacgggc atccctagaa atcgcaaggt
    31501 tatgctatca ctatatgcac tgacaatcgt aggataaacg agagctgctg caataaccat
    31561 agcgatgaaa cgatctgtat tgaacttttt tgcagcagta tatgccaaaa atataggcaa
    31621 gaaatagaaa aatgcatcag ctgctgcatt caaaatgata taagttcctg attcagtagt
    31681 taataaattc aaactcgtac ataaggcgag tactcctttg atcaaaccac ttcctgccaa
    31741 gacgcctaaa gtcggggcaa aaacagcggc gatcatgtct ataaaagagt ttaaaatgcc
    31801 ttttttctca gttcctctct ttccactaga tgcatcatct tctagtttca tttgtttgga
    31861 taatacgtca tacacttcat ttactgcatt tcctactacg acttgaaaca ttcctccagc
    31921 ttcgactaca gtgatcacac cttctagttg ctctaccttc tccttatctg ccttcttatt
    31981 atcttttagg gtaaagcgta accttgttgc acaatggtaa acattgctga cattttcttc
    32041 gcctccgaca aatcggacaa tatctttcgc taattgtgaa tagtccattt ttacttcccc
    32101 cttgttaatt aatgccgtag caatccttct tacaagataa gtatagttct tagaaaacgg
    32161 tttctccatt caattagtac cccttctaat agcgaacgaa actgtacaat agtgtgtatg
    32221 gtttacttag ccgagcaaat cactcactgc ttgaaagaaa acaagtatat cgtttctctt
    32281 atagtcaaag gagacaaaaa aaacacgctt gatcttcctt tgctgatgta taagtggtat
    32341 ctcaagctga tactctgtga tttttgtttc tacttggctt ttgataagac agcacaacaa
    32401 agtctcggca tttaacggaa gcataactag gtcagaacta ggagagatta gttgctgaat
    32461 ggaatctatc tttttatgga tttttatttg attttcttct ttgtagtaat gatcaagcag
    32521 ttttgttttc tttttttctt caactatggt cagcttttca cgcaaaatct gataaatctc
    32581 ccaagattca aactcatcta gaacactagg tagataaatc aaatcatccc actcaaaaat
    32641 aagctcttgc tcagtagtca aaattgcatc atattccgtt attttgtttg attgattttt
    32701 gaaaagatac actggttcta tccggcattc tttgatccac atgttcatac tttgttgaat
    32761 ctgttttttt agtgtattca aaatactaat tggctggtta ctaacaatca gtatctcccc
    32821 ttgttttcga aaaggattca acgcttcggc aagatacaaa acaaaataag cgctttcagc
    32881 taaattggga cgtaattgaa aatgtattgg gaagaaacgc cgaattaaat aaatttctaa
    32941 aggataacgg gagatgatct tttgtacaaa atgattcatg gtattatgtc cggcatccat
    33001 cctcgttttc atttgtttga tatgggttag caaaaattct gtttcatgga acaataacga
    33061 aacaggaaga tctaaaaaac gaataatcgt ttgctcaaat gctcttagat tcttctctat
    33121 ttctttgttt ttttcatcat taatcatcaa ataatttaac tctaatagtc gattttcaat
    33181 tgcttgtttt tcttgattgg aaaaagaata gggtagttct gatgataact tttttgtgag
    33241 ggtctctacc atcggaataa ataatgtata cctgtttgtt ttttctaaaa gttcttctct
    33301 tcgaagaatg gtcaaaacga gataccgaga aaatcgtatg aagtcctctc ctgaaacaat
    33361 ataattatat gatataagaa cctcttttaa tgtcttttgg acagtcggca tgatattgga
    33421 aaaaattgta ctgatttgtt gaggaaacct tgcgtgttgg atcaccgttt ctgttccaac
    33481 aagagataag aaaattcttt ttgaattttc ttcagcaatg atttttagtc cttttgtcga
    33541 cgaaacttcc agttctaatt taggattgcg ctcgatccaa cgcaaaatcg tttttatttg
    33601 taatgatacg gctgtttttg aaagaaagaa aatgtctgct atcttttgca tactgacata
    33661 ccctttttga aacaataagt acgtcacaat agttgaagct cggaaatcca tgctggaata
    33721 gattttatgt ttcgtcacct cttgtatgat ttgctgttta gtttcagatt caattgattc
    33781 taaaaaataa cctgaacgtt tatttgactg gataatgatt ttcttctcgt agatagcaag
    33841 catatttttg atttcagttt ggatcgtttt cttgttggta cctaaagctt cagctaattc
    33901 ttcgcttgtt atccaatgat tcttatttct taatactaca agaatttgga gttgtcgttt
    33961 atttaagtcc atttgcttac acctcctctt tgttataaac atacaggaaa aacctctcaa
    34021 atgacaatag tcacttgcag aggtttaaaa atcatttgaa gaaagatcta tattcaaggt
    34081 ttttaccatt agactggata atttgtttat aatattcaaa actatctttt ttcaatctcc
    34141 taaagcttcc gctcccatca ttttgtctat ccacataaat aaagccatat cgcttcctca
    34201 tttcccctgt tcctgccgat acgatatcga ttggtcccca ccataagtaa ccaatgattt
    34261 ggcaaccatc ttttattgcc tcagcaattt cctgaagatg tatttttaga tactcacaac
    34321 gttttggatc gtggattcga ttcaattcat ctggtgcttc atctagtcca atgccatttt
    34381 caactatgaa taatggtaat tcataacggt ctgtcaattc attcatgaca taacgaaacc
    34441 ctagaggatc aattggccaa ctccaaggtt cgggagattt ttccttcaaa aagggattat
    34501 cgaaaccatt tgctccgcca gtatctacgc gcatttctgc ttctttttta tagacagcgc
    34561 tacgataata actaaatgca atataatctg ccgtgttagc ttgcaagcac tgttcgtctc
    34621 catctaacat tactggtgca cagtcttctt ccttccatgt cctgtaaata taagctggat
    34681 aatgccctcg gcacatgaca tcggagaaaa accatgaatt gcgtttaaat tgcagtgaac
    34741 caaatacatt ctctggatca cagtcatatg cataagtcgc taatgcagaa aagctacaca
    34801 taatacccat cttagcattt ggatcgattt tctttaataa ttgagtagca cgggcgttcg
    34861 ctagaaacat atgatgacag gcttgataaa tcactttttt tgttagtgta gcaatgggtt
    34921 caagtttatc tctagcaggg aatgtaacca atccagcagc tgcataaggc atacgaaaag
    34981 cattattgat ctcattaaag gtcatccaat atttcacttt cccttggtag cgttcaaaga
    35041 cggtctgcgc ataaaattca aaaaagtcga ttaattttcg attttgccac cccccatact
    35101 ctcctaccaa tgctaagggg gtttcataat gactaagagt aattactggc tcgatatcat
    35161 atcgatttaa tgtattgatt agtctgtcat aaaatacgag accagcctca tttggcttct
    35221 gttcgtctcc tcttggaaat attcgtgccc atgagatgct tgttcgaaaa gcttttaaac
    35281 ctaactcctt cagtaaacga atatcctctt caaatcgatg ataaaaatca atcgcttcat
    35341 gagaaagata atggagtgat tcgtctaaag caatctccca aatctttttt tcttcattcc
    35401 actgaatact aggatgctgg ttcatgatcc cgataagcgt atctgtcaca ttaggtaatt
    35461 tgccgtcttc caaccatgca ccttcacatt gattagcagc tactgctccg ccccataaaa
    35521 aattttctgg aaatatttcc tgatccataa ctatctctcc tcgctatcaa caatttctta
    35581 cttgaagtat agctcgattc atcaaaacaa gatattctaa ctatttcggc agaagagaag
    35641 aaaaaaattg aatgctactc ttcattttta atatttatat ataacaaata cataagacga
    35701 tccccgaaag aaaacactcg ttaaccatct aagcaaacga tttattttac ttccaacaca
    35761 aatgcttcat aaggtcgtaa atcaactttg ccgtctggta actgtttcag aggataattt
    35821 gacacaataa cccttaaagc ttcacgtgga atagtgaacg tctgcttttc actggacata
    35881 ttacaaataa cgagccattg ctggtcttca tatatgcgag aataggcaaa gacatgctca
    35941 ggcgtctttt ccactaattc atagtctccc cagacgatga tgtcatgttt tctacgcaaa
    36001 tcgatcaatt tcttgtacat ataaaaaaca gaatcttgat ctgctaacgc ttcttctgca
    36061 ttgatttctt tataatttgg attgactcga agccatggaa ttccagtagt aaacccagca
    36121 tgttcacggg cgttccattg cataggcgtc ctagcattgt ctcttccttt cgccttgatt
    36181 gattccaaga tttcctcttt tgtaaatcca gaagaaatcc tttcgttata catattgatt
    36241 gtctcaatat cttttgcttc cgcgatatca tcgattgggc aattcgtcat gcctaactct
    36301 tctccttgat agatataagg ggtgcctttc atcatatgga gcaagatagc aaacatcttg
    36361 gcactttctt ttcgatacac atcgtcattt ccccaacgag aaacaattcg cggaagatcg
    36421 tgattattcc aaaataaact attccatcct tcatcaccta aagatgtttg ccatttggat
    36481 aatacttttt tcagttcaga gatactcaag ggtttcaaat cccatttgtc tttgccttct
    36541 tgctgatcta atcctatgtg ttcgaattgg aagaccatgg ataattcatt tcttttagga
    36601 ttggaataaa gtttagcgat ttcggtagtt gctccccatg tttcgccaac agtcatcaga
    36661 tttttgtcac caaaagttgc ttgattcatt tcctgaagat actcatgcag tttaggtcca
    36721 ttacttgtga tttctttatc cggttctttg ccaatcatat cgatcacgtc catacgaaag
    36781 cccccaatac ctttatcgat ccagaaattc atcatttgat aaatctcttg gcgaacctgt
    36841 ttatttttcc aattcaaatc aggttgacgc ttgctataca agtgcagaaa atattgcccc
    36901 gttttttcat ccaactgcca agcagaacca ctgaatattg agcgcaatct attaggctct
    36961 tttccattga ccggatcacg ccaaatataa tagtcccgat aaggattttc tttattttta
    37021 cgggcctcaa tgaaccacgg atgttcatca gaagtatgat tcaccaccag atccatgatg
    37081 atacggatat ttcttttctt tgcttcttca atcagttctt ccatttcttc catcgtaccg
    37141 aattctgcca tgattgcttg ataattactg atatcatacc cattgtcatc attcggagac
    37201 tcgtatactg gagaaagcca aattgcatca atacctaaat acgccagata atccaacctg
    37261 gaaataatcc cccgcaaatc tccaatacca tcttgatttg aatcttgaaa acttcttgga
    37321 tagatttgat aaacaactga atggtgccac catttttgtt ccatgatttt cctcctataa
    37381 atggatgttt ctattttttg cttaatcatt tattttagtc tctctcacta aaaattgtaa
    37441 cgctttcaaa tagctattgc accttgttat cggtaacaga ataaaacaaa aaaacagctg
    37501 aggaaattcc ctcaactgtt ttttgaaagg attgatttat gcagggaaga atgctgattt
    37561 cacaacttct ttataaacgg tgttcactca gctgtccctt ggtattagct caaaaaacgg
    37621 caaaatataa gaaactattt tttcgttttt ttctctaata ctcaggccag aacgctgatt
    37681 tcacaacctc tttatttcac attcaatatc cacatgaatc ccacaaatac aaagaacaga
    37741 ccatacatga ttggagatac ttcttttcct cttttagctg ctaacatggt aattggatag
    37801 aagataaaac ctaatgcaat cccatctgaa atactataag tcaaaggcat gccgatcaag
    37861 atcaagaatg aagggatcgc aatctctaag tttccccatt cgatttgtcg caaagattgt
    37921 gccatcaata ctcctaccat aatcaatgct ggtgcagtta cttgtgaagt aacaactgat
    37981 agtaatggag aaaagaacaa gcctaacaag aagaaaatcc ctgtagtaat agctgtgata
    38041 cctgaacgtc ctccaacggc gataccagct gatgattcta caaatgcacc aacaggagaa
    38101 gtaccaaata aggaacccgc aagcatagct gttgaatcag aagctaaagc tttccctaca
    38161 cgaggcatct tgttgtcttt catgaaacct gcttggtttg ctaatccaac taaagttcct
    38221 gctgtatcaa agaaagtcac aagcaagaat gtcagcacta cgacccacat ttgaagagaa
    38281 ttgatgtccc ctacatgttt taatgcaact aaaaatgttg gtttcaaact tggtgctgcg
    38341 gcaacaattt cagaaggcat tggaatcaaa cctgtgacca gacctaaaat cgttgtagct
    38401 gccatcccga tgaagatgcc acctggtaca cgccgaacaa gcaagatagc agttacgact
    38461 aatccaaaaa tcgtcaacca agtagaacca acgttgagtg atcctaatcc tactaatgta
    38521 gattcattcg aaacgatgat cccaccttcg ctcagaccaa ggaaagcgat aaatagaccg
    38581 ataccgccag aaatcgcata tttcaaatct gctgggatcg catcaatgat catttcccgc
    38641 agcttgaaga ttgtgatcaa aataaaaatc aaagaagcaa cgaatacgcc ggctaatgct
    38701 gtttcccatg gaatccccat tcctaaacaa acagaataag caaaaaaagc attgatccca
    38761 agagcaggtg ccgttccaat tggataacgg gctaataccc ccattaaaac acagccgatc
    38821 gcactggcta atgcagtcgc agtaaacact gctccttcat ccattccaga agcacctaat
    38881 acagttggat ttacaaataa gatataagcc attgagataa atgttgtaaa tcctgccagt
    38941 acctcacgtt ttaccgtggt gttcaatttt tctatttcga agtaggaaag tattttttcc
    39001 ttcatcttcc atccccctaa atccgaacat tataaataaa gtccgttaaa atttcgattt
    39061 attaataaaa caacgtgttt tatcatatac taaaacaaaa cataagtaaa caaaaaattc
    39121 ggattttaag caaaatccga acgttccatc tgattaaatc cctttatctc aaagttcatg
    39181 aacattcact ttggattaca agaatttctt tattgtgtta ataaagtttc ttcattctag
    39241 tctcaaaaat caatagtgtg atttgatttt accatttcgt ttctgttctt ctttgatgaa
    39301 ttcttttgta tcgacaggat tcagattatt tagtccgcca aaacctgtat cgaccatttt
    39361 tccccagcct tttgtcttct tttttcctga tttgagattc tttgctcgaa gcttctcact
    39421 atgttttgct cgccgatact tcaaataacc ttctaatctt cccattagat ccactgtttt
    39481 tcctcctttt tattcaatca gacgactcag cttttcctat aacaaaataa ttgccttcag
    39541 ggtcagaaaa attgaaagtc aagatgccat tgttatctgt gatttctcct ggttcttcca
    39601 tgttctcgta gatagtttca aattcatccg taaaaaatac taaagaaggc ggtggtccta
    39661 atacctctgg tgaaaacttc tgcacaaata cttttgggaa tacgcctaat tctaattctt
    39721 ttgagatgct gagtacaata ctcttaaatt cttctggcaa ttcgattgtt tcagaaattt
    39781 ctgcaccaat tttttctaca aagaatcggc aaatcatctc tacgtcattg acataaagca
    39841 tcacacgtgt tttttgtatc atcatttgac ccttctttct atttgatttt ctttcctgtc
    39901 tataaactga ttccattata tcacgaaagc gcaaacacct tgtgtttgat actattcaag
    39961 aaaaataaag caatagtttc taggattgct ttcctccaca aacttttttt acaaaaaaaa
    40021 aacggcaatg aatgccgctc ttcttatttc gattctttta taattctgct tctgattcat
    40081 ctttggttag tgtgtgtcta acactagtta cgccaaatgt ttgatcgatt ttttccttca
    40141 acgcttcgat tgcctgtgcg ccacgtgccg cttgttcagc attgatggat tcaatgacca
    40201 gcaccgctgc ttgtgtttct tccgtcagca tcgttctttg ggcttcacgc cagttgagac
    40261 agcgacagat cgctccttgt tcatcatagt agatgatttc ttccggaagt gccggagcat
    40321 cggaatctgc acctaaaggc aggaatgatt cgccaccttg ggcttttcct aaatgcagat
    40381 ttcctgcgat cttttcgata tcttctccgc cgcaaggtac gccaaaagac aaggaaatac
    40441 tattatagat atctacaagc ggattgattg ggaaaaattc ccgttcttga tttgcacgtt
    40501 tcagtaaagc ttcaatagaa gaacgtgcac cttttttagt cttgaatgtg gtaaatgcct
    40561 gacgccactg agcaatgact tcgttttggc tgaatgtttc ttctgtaagg taatttcttg
    40621 cttcttttgc tgcctcattc aataattgat gatacgtttc ttcactttct tttgttacgt
    40681 ggttatcgat accatttacg gataagattt cgatggttgc ttcaggaaat acttcccaga
    40741 aattggtatc cactattact tttttcatct gttcacacct cttgattaga ataattgctt
    40801 gatgaataga tagtctgttt ggggatcatc tcccatataa aaaacatgtt cgctgtattt
    40861 gataaatcct tttttacgat aaaaagcgag cgcaacatcg ttgtgttccc aaacaccgag
    40921 ccaaatggct gttttttgct tttcttttgc tttttcaata gctgtatcta gtaaaaatgt
    40981 accgatacct tgatgtttaa atttttttcg tacatagatt cgctcgattt ctaaaaaatg
    41041 atcagccatt tcctcagatt gactcgttcc tgtatttagt ttcaggtaac ccgcaggctc
    41101 ttgatcgata ctcactagga aaaaatcggt ttctttatct tttagctctt ctgtaagttt
    41161 ttctagtgca taagcttgtg tcaagtaatc agctaaatgc tccggtgaat tatcttttcc
    41221 aaatgtatct gtaaatgttt cgatactaat ttgttgtagt aagtctacat ctgaaggcgt
    41281 catttttcga atttcgtatt tattcacggc ttcttccttt caatagatcc ttttattgcc
    41341 ttttttgaca aaattccaat cttcgctaac attcttgcat actttattta aaagatattc
    41401 caattgtttt gcttcttcgt cagaaagacc atttaatgcg actgcattgg agtagttgtt
    41461 ttctcgaatg atgaaaggat agatttctgc tcctttttta gtcggataga gtttcttgat
    41521 ttttttattt tctttatctt ccaaacgttc gatcatccca ttggattcta atttcttgat
    41581 tgctcgggca gtcgttgtac gatctacttt gatcatttct gctagttttt cttggatgat
    41641 tccaggattt tcgcaaatac gtactaaata aagatactgt cctcttgtta aatcgacttc
    41701 tttgaattcg atattgctaa tagaatctag cgctcgggca atcattccga tgtctcgaag
    41761 gatttcagcc atttcatccc acctttattt tgtttgtaag actagttcac ctctttttgt
    41821 tgcaaatgca actattttca ataaaaaaat caagttcttt tttaataact agtaaagtat
    41881 attcttttct tcttttacga aaaagctcaa tcacaataga tctggttttt gttttctaaa
    41941 ataagtaact gtttcttcgt tgttcgcttt tctgatagct ttggcaactc ttctatacta
    42001 aaaaatccca tgttggaagt ttcattattc tcaataaact tggcctcttc accgtggatc
    42061 gcacaagcaa aaacaagttt ataatactga aaaagctgtg gaatatcttt ttgtttattc
    42121 gtatcaaaaa ctgctcgcaa ctgagtagtt tctactgtta atcctgtttc ttcatagact
    42181 tctttacgca cattttcttc aggagacaat cctatttcag caaatccacc aggaagtgac
    42241 cattctttcg tccgtttatc ttcgattagt aggaccttgc catctttttt gatataggca
    42301 cgaacatcta cttttggtgt tggatgtcct tctccaatcg ttgtgagcgc ttttatttct
    42361 tcgaaagttt cttttcctat ttcactgatc agttccaatg aaatcgtccg gatctcctca
    42421 aaacgctctt tatcaaatac atcttttcca taatatagac cagcttgaga aagtgcgagt
    42481 agtcttttgt attgagccaa cttcttgttc attctcttcc cttctttttt agcaaatgta
    42541 tgactatgta atcaagtata gaagactggt ttaattacgg caaataaata taatgaaatc
    42601 tgcaataaat aaaagaatgt taaaaaactc gagaacaaaa gacattcgac ttctctgggg
    42661 ctgtgaataa atggcgttca aaagcttgca tctgcggtaa taagccccac caaacgaaaa
    42721 atatgacgag ctattcttgt ctggctgttc ttattaattg aggcaggaca gctttttcac
    42781 aacctcccca ctaaacggtg ttctcctacc aactcctcgg tcacaagtca caatattcat
    42841 caaacatgag aagctgtttt tttgaaattg ctccttgtga ctcggagctg aacgacaggc
    42901 tcacgacctc tcggtaaaca gcgttcaaaa gccaattttt cgatattaag ccatttcttt
    42961 gttacttatt gtcattgatt tctttttcta atttatccaa tagtggagtg taatccactt
    43021 gagtatagtc tgttacatca aattcatata gtgttcctaa ctggaatttg tcatattttc
    43081 ggtcggcgat gatctttttg aatgcttctt tcgtaatatg attggtccct ttcgttcgat
    43141 cttccaatac gtcaccatag tgataatgat ccatgatata gctcaaatga cgatctgatt
    43201 cacgatcacg tttgtaccga cgttgccata acacttcaaa gtctgctgtt aggcgaatcg
    43261 tgatcacctg atatccgtat ctatctgcta attcttggag tcgtgctttt tgcttatcac
    43321 taaaaggata ctcggacaca atcactttct ttcctgcttc catatacaaa tcaagtgctt
    43381 tgtaatagaa aggataaact ttcgtccttt cttgttccgc ccgttcttcc aaattgttaa
    43441 acccgatgga ttctgcatag tattccttga tttcatcgag cgtgatggca tacatatctg
    43501 gaaatcgttc ccgtagttta ttcataagat atgtttttcc tgtaccagga ctaccagcca
    43561 gaagaatgaa atactttttc atctcattta tttcctaagt taattggact ttccaagttc
    43621 ataaacagtc caatcttatg cttgacaatt tcttttactg cttcatttgc tggaatcaca
    43681 ttatggcgaa gagacttgtt gatttctgtt tcactaaagg tttctttagc tactttcgtc
    43741 cagttgacta aaagctccgt tccaacattg aatttgcgga tcccgttatt gatcaattca
    43801 ggataatctt cttctttcac acctgttcct ccatggataa ctagggggac atcaactact
    43861 gcatcgattt ctttcagcaa gggaatgttt acttctgttt ttgatttgaa ttgtccatga
    43921 ttcgtaccga tagcaatagc taatgcatcc acgcctgtag cttctacaaa tttcactgcg
    43981 tcttccggtc ttgtatatac cttgtcattt tcagcgacat ggattccttc ttctgtaccg
    44041 ccgattgttc caagttctcc ttctacggaa acaccgtgcg catgggcata ttctactact
    44101 gcttttgtct tcaggatatt ttctttgaaa ggcagatggg acccatcata catgacagaa
    44161 gtatatccgc tgtcgattgc ttctttgata tcatcaaagt ctcgggcgtg gtctaagtgc
    44221 aagactgcat caaccatttc ttcttctgca atagctgtca ttacattgac taacacatgc
    44281 atgccgatat atttagcggt atcgacactt gtttgaataa tgatcggcga acccatttct
    44341 tttgctgcgc ctagcatagc aggaagcatt tctaggttat gcgtattaaa agctccaact
    44401 gtaaagttca actcttctgc tgtttttgtt acttctttca atgttgtata catcttttat
    44461 tgccacctta tctttttatt tgtttccttt gatcgttact tgttttgcaa tttgtcgcat
    44521 ctcatccatt ttgcccatgt agtaatcgat cccttgatca tcaccagcac gagcagcatc
    44581 tgcacgcttc tggttataga ggctcatcag ttttttatac ccatctccga tcgtctgttt
    44641 attttccaaa ctcttttcta aatattggat ggcttgatcg ttcacataca attcagaaaa
    44701 tttaagtcca acttgttcat aaagtttcgc taatttatgc ttatcttggg tagtggaaat
    44761 ctcttgtttc aattgtgtaa tctctgattg aatcttttcg gtcgcttctg gcgatagggc
    44821 tttttcttct tttgccagaa cttttttcgg ttctttttct tttttcttaa aaaatccaaa
    44881 catcattttc cctactttca attaaaatgc tttcaaaact gggcttaaga tccaggcata
    44941 taagagtgct gcggctactg tatctacatc ggttgctgta gcattgttga atcccatcga
    45001 attaaagatc gttactagca aagcgggcaa taatgtaata aagaaaccat gagcaatacc
    45061 gccaatgatc gctcctcggc gtccgccaac tgcgttgcca aatatccctg ctgtccctcc
    45121 ggcaaagaaa ttggttagca taccaggtaa aatcatcgct aagccgaaca tgggcaaagt
    45181 aaacatggca ataatcgtac caatcgttgt cgtaatgaaa cctaaaatga cagcatttgg
    45241 gctataagga aagaaaactg gacaatctaa ggcagggatc gcatcaggaa ctaatttcat
    45301 agctatccct cgaaatgccg gcacgatttc acctaacagt agacgtacac cagctagcaa
    45361 tacatacaca cccactacga attggatggc ttgtaaaaag gcaaacatga tatagttttg
    45421 atctcctgat aattcagaag cgaatggttc gcctgcaaat aaaacagtga tgatatatag
    45481 cgggaccatc acgaccatga cagacaaata agtatcttgc agaaattcga aatgtgtcgg
    45541 caacttgata tcttcgatcg actttttatt ttcgcctttc tcaccaaaaa gcttagcaac
    45601 accggcttca aataagtacc cgatcgtaca aaaatgacct aaagcaatat catttgatcc
    45661 agtgatttta cgaatgatcg gttgcgctac tgccggcata gcaatggcga aacaagctcc
    45721 gataaaacca ccgaccaaga tcaatacgat ccctcgcaga ccggcaaaat agccaaagac
    45781 tgtcgtcatc gttgccatcc ataaaattgc ttgaccagtt aaaaaaatgt acttccattt
    45841 tgtgaaacga gccaaaataa tattgaatac aaaaatggct aagaacgtca gtgcaatatc
    45901 tctacctaaa cccaaatcat tcattgcttg accattgata gattcaatag aagggatgat
    45961 cccttgcata tgaaatcctt cagtaaagat tttcccgaaa taagttaaac ttccaacgat
    46021 gatgctcgaa ccggcactca acacttgaaa tcctaggagt gtctttaacg tgccagagat
    46081 gacttgtcct gctgatttct tctgcagtag tagacccaac atagcaatca atgcaatggt
    46141 gatcgatgcc tgagtcaaaa tattttcgat gataaaattg ataacgccca tgtttttacc
    46201 ctcttttctt tttgtattta gataactccc ttatcttgca gaactggggt gattttttct
    46261 tcaatttcat tttttgaaac aatatttttc aaatagatca tcgccgtttt aggatcaact
    46321 gcgaactttt cgaactgcga acgaaaattt tctgctgtga tgatcacatc tggtttcatg
    46381 gaaactgcgg aagaaatatc acaatggtcc agtttggctt ctactcctaa tttgttcaaa
    46441 acatcctcag cagacatctg tgctgcaaaa ctacttccta aacctgcacc gcatacgaat
    46501 aaaatgttca atttgttcat ctttgttctc ccccgtatca tttattattt ctcaactact
    46561 gaaaaaagca gttgtttgaa atcttcgata tttgtgcatt caatcaatcg atccaacttc
    46621 tcttcgttgt tgatcaattc aattagttct ttcatgatgt tcagatgcga gtaggagtct
    46681 actgctgcca aacaaaaaat aatctttact ggatccattt caggattacc gaaatcgatt
    46741 ggttgctcaa tcgtagctac gctgactcct aatttattga ccccatcttc tggtcgggca
    46801 tgggctagtg ccatgtgttt gccaatcacg atatacggtc cgtaatcatt cactgcacga
    46861 atcatcgctt cgacatagcg ctcttcgaca atgttttctt taaccaatgg tctagcagct
    46921 agttcaatcg attcctccca agtagtggct ttttctttga tcatgatatt gctgtccgtc
    46981 aaaatatctt ttaacatagg ctggatctcc cttttgttga tttctaaatt gtttgtttcg
    47041 aataattttt ctaacttgcc atagatgatt cgatctactc ggccaccact ttctttcatg
    47101 acatctatta tttgagtgaa taactccgtc gattcatgac tgttaggctc taaccgctgt
    47161 agttcttcat ttcttttcaa aaagtcttct ataaccaaga tattcgtctc agttaaaatc
    47221 ggatcgacaa ctaaaagcgg cttatttcga taattcaatt gaaaggtgct gaaaaccaga
    47281 ttgacatcca atttatctac taattcgatt tcttttgaac ttaaaactgc agtaatctcg
    47341 atctgaggaa acttttcttt cagattctct gctaacagat ttccagtagc catcccatgg
    47401 ttacagacaa cgactgattt ataaacataa ttcaagtctc gcttgattac actggcgatc
    47461 gtcgaaaagt ggatgacaag aaaagctatc tcgtcctcca gtatcttgcc accaattact
    47521 tcttcgatcg taggaacaaa atgagtgact gcgcggtaga ttgggaaata atttgttcgg
    47581 atgttttctt tcaatggatt catgacttgg atatgattct tgattcttac aatcagtgca
    47641 gccatgtgct tatagagatt ttcgaataaa gcttcttcct ttagatggaa aggtacgtgt
    47701 gtctcgttct ctacaaattg gatcaattgg atcgttagta attgagcttg tacccactcg
    47761 attgaattgg agatatctct agcattgaac gtctcaatag tgaaacgaag ataatttctt
    47821 tctacttgcg ggacattgtc caattgaaat tcctcgatca tgtggtcaat aaatgcagaa
    47881 aagctactgt cttttatttc tgtatttttc caattaatgg cagagataaa ttcatgtttc
    47941 ctgattcgct ggatccagat caatgtaaaa agcaggattt gttttttata aatatcatct
    48001 tcttgtttca acaacttatt ggaatagatc tcctgtattt ttttgatttc caccaaagga
    48061 aaataatgga gaaagatttt ttgagccggt gttaattgtg catcttgatt cataaagaag
    48121 tcggtcattc ccagattctg attgataaaa tcatagatca tcgtacggat agatcgttct
    48181 gaacctttat aaaccagtcc ttgctttcca taactgacga tctctatccc ataactgacc
    48241 agtgttgctc gtaaccgtct catatcttca tctaatgtac ttttagagat ttgaaattct
    48301 tcttcttttt tgtttagatg gatcgggatt cggctgtaag caacggctaa aactagatca
    48361 aaaatacgtt cgcgccgatt caaaaagccg tctttcacat cttttatttc agtttctaat
    48421 cgagcttgct tttccttttc cagcttcaac aaaaaacctt tgttgcgaat tgtacggatc
    48481 tccggaagct ttttactgct caaccagata ttcgcttcaa gaacttcctg acggatcgtc
    48541 cgctgactga cattgaactc acttgctaga tgttttgctt ttactggata ttgggctgtc
    48601 atgattctgt gaagtatttc aattgttctt gttttcatag aaatgatctc cttctgattt
    48661 caagtatatg gttccgctta cataataaaa agtagatatt tctgccactt gaagtggaaa
    48721 gaaagtaagc aaagcaaaca aaaaataagc catatgcttt ttcatctcct caacgaaaaa
    48781 gacatacgcc tattattaca aaaattttgc ccatctatac aaaaaagagg ttgtgaaaaa
    48841 gctgtcctgc atcaagtaat aagaacaact aaactaaaat agtgcttcct atttttcgtt
    48901 tggttgttct tattatcgca gatgcaagct tttaaacacc atttagtgga gaggttgtga
    48961 cagagatgtt cagccttgag cgataagaag cgattttgaa aaatcgcttc ccatatttta
    49021 caaaatcgtg ccttattgcc gataggctat ctctggcaca ccgtttattc agaaaaagga
    49081 gctgcgacaa ggcttttgtc acaactccgt tcataaatat ttcctgtcaa tccagcataa
    49141 tcggctgaat aaaatcggct tgccatcttt tttctttctc agaaaaatca aaataccata
    49201 tctgagaaaa atactgtcgt tgtgtcttac ttcctgagat caatcgccca tcttttcgat
    49261 cgactgaata atcacagcat ctgaaatcaa ttcggatggc aaaactattt tcgtggatct
    49321 gtcgataatt tcctacttcc tgaatagtta tttttttagt atgatttctt acaccgtctg
    49381 ctttgttttt ttgcagaact ttctgatgtt cggcaaacag cttgtctgta taacagcttc
    49441 tggctttttc aggagtttct tcttcccaag cggattggat cttcatgaat atcttttgga
    49501 tttccagaaa ctgtgctttc ttttctttag ccgttcctga catgcgggac aatatctccg
    49561 ctttcagcct tttcttttgt cttttattct tgacgataga aaaagcagaa acccctccta
    49621 ctgacaaaac agctaatgca agaaatcccg tttgctcata actagaagga cctgtacctg
    49681 gaccataata cccataagaa tctctgccgt aagaacgact gccaaaataa ctgcccccat
    49741 atgaacgacc accaaatgaa cctctgcccg aacttcgacc gctagatgta cctccaccat
    49801 gaccacccgc gacagcgaat ccttgggtcg gcagagcaaa caaacatagg cccactaaca
    49861 aaatgattgc taaataattg atttttttca ttgtcttcct cgttatcttt tattacaaaa
    49921 aactattttt atgatagcct tgctaaaagg aagagtcaat aaccagatgc ataaactgtt
    49981 tttttagcaa ttttgtctta tttagtcatt gagtaacatc ggacaaatgt atgtgtcggc
    50041 agtccataat tcctctatct tcagcatgaa caagcttgca gacaagagat ctgcgcttcc
    50101 tccaggagag atttggtttt cctgacaaaa gtgcaacagt gctgtaaatg gacgatttgg
    50161 taaagcactt tgactagcag catgagcaag tttttgcatt tccactaacg ttttctgatc
    50221 actgcgatgc aatacgcatg tgtcatctag cgaactcatc aaaactaaca acatatgcat
    50281 ttgtttctgt acatatgaat cattatctag ctccttagct gcttgaattg caagcctgat
    50341 atgcggatac cctaatatcg cttcttcata tgcaccattt acgccatatt tttgcttcac
    50401 cctatgacca tgagtggttt ttttatgacg atattttgta tcaggaaaag cagcaagctt
    50461 gctgacatca tcaagtattt tcgataaaga aaaaggctgt tccatactga ttttttgtgc
    50521 aatgacactg atcaatagcc ccattgccca aatcgctcct ttatgggtat tgatgccatt
    50581 tgtagcaatc agcattgcct tttccgcttt tcgcccaatc tcagcaattc tttctcttaa
    50641 ctcctgatca atagcatgat gccatgctat ttgagccatt tcttcaaaaa aaggagttag
    50701 cgtcttagca gacttaagaa ataaagcata gtccatatcg gaatgtgcac ccgcatccag
    50761 tgcatccact aatccaggtt ttggacttaa tgttacttct gcgatcaatg cttcctctac
    50821 caacgcagcg agttgctgag tcactgcttt ttttcgttcg tcatttatca tgacactcac
    50881 tccttcttcc tactattact tgctgaaacc ttttttgata gctacccaaa tgatatgaag
    50941 taaccagctt aaagcaaatg cacaacttgc cgtcattaga taagctacaa gaatcatgac
    51001 tcctgaaggt aactgcagcc aactatcttt aaacaataat aacagtactt ggaaaacaaa
    51061 tacattggct aaatacgtac gataagcata aacagacaac catttgaaca atggcaattt
    51121 tggcgaatcg tttacaatca ttttggaagc gcctatgaag acagcaaaga tgatgactaa
    51181 actgtataaa ctttggatcg tattcaagta aggtgcattc gcaaagctca agtctcctgg
    51241 atgtgcgagc aatgaacgga cagaaaagaa cgcgataact aaggcaacag gtaagaaaag
    51301 ataacgtact ctgtccaaat attgatagac tttttcgtga taaacaaatg cggctacccc
    51361 ataaatcatg aaactagcaa caaaccgatc taataagtac cagctgtcat gataaggccc
    51421 agtcaatact tgtgtttgat agaacagata ccatgcgaca tataaaatcg ttgaaccaat
    51481 caatactggc catacatatt ttttattagc aaatactttg taaccgagcc aaacaaaaaa
    51541 cggcatgaac aattggatct gcagcatcat aacggtatac cataaatgag gcgctccatc
    51601 acctgtgaca tatttcagta aaaagcttgc agtatccgta tacggagttt gttgttggac
    51661 tgctggaaat agccaaagat aagccgtcgt ccatagaaga tacggaacga ctagttcaaa
    51721 aaacttttct ttcaaaaatt cccaataaga caaatgctga cttgttttta ccatattata
    51781 aacaattgca aaaataaaca ttggtgctgt gtatttaacg atgacgtaaa aggtaccaag
    51841 aaaagctgta tttgttgcgc tgtggctgtt tgccaaagca aagctcaata tcgtttgcat
    51901 cataacagcc gttacagctg atactttctg gaagtcactt acgttgattt tgattttttt
    51961 gttttcaaac atctctttct ctctcttttt ctttattacc aaagttcttt ggttaattta
    52021 ggtccttcga ctgttttcaa tagaattttt ttctctggct ctctcaaata ctcttttagt
    52081 gaaaaaccgc cttgccgagt cactacttgg gtatcgaatt gctcaaaaaa agcaggatgc
    52141 gattgaatta cctgaattgg caattcaatc ggtgaatttg cgtacagcag caaatcaaaa
    52201 tcactggttt cttttactgc cggaatccca gtcgataatt caaatcctaa gctgccgccg
    52261 attccccatt tacatcctga taatatgttt ctagcagcat gaaattgctg ataaacagga
    52321 taatccatga ttttctcttg tctatacgat tcttgttcga tgatgtccca cggatgaatg
    52381 acttgtttga cggcatgttt tgagatttct gttgaccatc tctgattgcg ggcaggtcca
    52441 cgtaaaccaa tcggtagtat ttctgcgtga tccgtcgacg cacgacgaac tgttccgaac
    52501 atcttttcag ttagaacgac ccattcaggc aaggtgaacc cttctatttc atgtttatca
    52561 aaaaggacaa tatcatgtgc ttgaatcatt ttttatgcat cccattcttc gtccattttg
    52621 gcacggactt tatttgtttc gacacgtccg aatgttttgg ctgtttcgtt tgtgtatcga
    52681 tttgtcagca tcgtgtcatt tgtattgcgg acatcttgaa tggcttcatt gattgcttcg
    52741 atgactttgc tgacattttg ttctgaagca gcttggtcgg tgatatcttc taggaaatga
    52801 tagatcgctc ctagttttgt ataattattg atatcgtaag cgatagctgg aactttttca
    52861 gcagctgctt cgatttcagc aaccgaacgt tttgtcacac gtgcagcact ttctttagac
    52921 atcgcttgga cggtaatttc tttattgttt aaagcaatca agcggcttga ttgtaatcca
    52981 tgtgctaaaa atgcacctga aatagcattt cctgcaatca atccaataac tggatgtcca
    53041 gcttgacgga gttttgcata agcagcagca ctattagcca aagccacatg gataccgatc
    53101 aattcttcgt tatagccgta tgcttggctt ggtacatcga tcacaacgat gattggccgt
    53161 ttcacttttt tgtctttatc ttcttcgtaa acgtgatcca aaatttgtcc catgcggaaa
    53221 ccttcaacta aaccaacttc accgtttcgt acacggtaca tcgggttttt aggatcaggt
    53281 acgattgatg tcacgatggc ttcttctatg aatttacttg atttaccata atacacagta
    53341 ctaatagaag aagttggatt tttcatgcca gttagtgccg caaaccaatc gtaaccaaca
    53401 cttgctgcag ctggttcatt tccttctgct acttctggta agtcaattga ttttgcttga
    53461 tggttagcaa ttgcttcatt gtaatcgtca atggccattg gttttgaaag atctaaagaa
    53521 tcaagtaatg ataaataaaa tgctgagctt tcactgcgat gcgtgtcttt tcgttcgtcc
    53581 attgctgcga tgacagcagc tttgattgct tctacactat cagtcactaa ttcatcaatg
    53641 atttttgatt ctacccgttg acgagatcct aatgtattcc aaatcaagtc tttgtcacta
    53701 gaatcaaatt ctcgaacgcc tgcttcttgt tcaataactt ctggtccatt caaaccgact
    53761 ctcgcttttt ttgttgcgat taaataactc attaatgctg aagtaatcga cataccgcca
    53821 aatgatccta cacgtcctgg aatcaaaccg atgactggta catattgttt tagagcaatg
    53881 attacatttc cgatttctga aatagataac agtccgtagt ttgcttcttg caaacgaaca
    53941 ccacctgtat ctagtacaat gactggaaag atctcatttc cttttttatt ttcttctaac
    54001 acatgttcaa gtgcagcaac gatttttgca ccggaaactt caccgatacc gccaccttgg
    54061 aattttcctt ccatagcgat tactactgct tgtttgccat tgatcgtgcc acggctgaca
    54121 acgatcccgt catcactttc agggacgatt ccttgtgctg gtaaacttgg cgcgatgatt
    54181 tgatcgaatg gatcaatcaa ttcacgtcca ttgtcaagta gagcgttcac tcgttctctg
    54241 gcgtttaatt ctacaaaact atttttcatc tgaaagttcc tccattgctt gcgtcaatcg
    54301 taggtacaca actcctggtg tagcaccgaa gtcattgatc acgatatctg ctgaaattgg
    54361 ataacgatca aaaaatctcg ttaatacatt gttccatact tcgccgaagc catcgcttcc
    54421 tgtacaaatt tttacagtag attctgtttt ttctgttgga aacatcaaga tttccaaatc
    54481 tcctgaaccg accactccta catggatttt cttattgatc ggctgtttcg ttggatattt
    54541 ataagctaat tgttccatgg atctctcccc tttttgtaaa acagagcata cacgttctgc
    54601 ttattcgtct ttcttcttcc atctgggtaa atggcgacaa aaggtttttc tcgccaattt
    54661 tatgaccaag ttctgaattt cattggtggt tcatacaggc catctgacca ttcgatcaaa
    54721 tcatcgatgg tttgtgcagc taataatgaa cgtttcgctt cactacgttt gatgcctaga
    54781 tcttctggta atttcacgat gccggctgca cgcaagctat ctagtgattc tttcgtgcta
    54841 gtcattccaa gaggtgtaac accgccaatt gctgcaattg ctgcttgtcg ttcttctttt
    54901 gtttttgctt tgtacaaata agcaattcct tcttctgtca cgatatgtgt agtatcttcg
    54961 ctataaatca tcactggtgc attggctaag cctgcttgtt tttgaacagc tagtgcatct
    55021 aattcttcga caaagactgg ttttttattt gaaccatatg tttcaaccat ttgaacaact
    55081 aatttttggc ctttccctaa tggatcatca ttgtcgcgaa gtgattgcca tgctggtgta
    55141 gaatgacgac gaccgccagg attatgtccc atgttaggcg caccgccaaa accagataaa
    55201 cgtccttttg ttacggtaga agagtttcct tcgtaatcta attgcaaggt tgaaccgata
    55261 aacatatcga tggcatattg tcctgcagct tgtgataatg ttcggttact gcgcatcgtg
    55321 ccgtctttac cgacaaaaaa gacatctggg cgagcagcaa tatatttttc cataccaact
    55381 tcgccaccaa aactatggat gctttcaacc caacctgatt cgatggctgg aatcatcgtt
    55441 ggatgtgggt tcaataccca gttcttcgcg attttccctt ttaaccctag tgattcacca
    55501 taagttggca gtaacaattc gatagctgct gtattgaatc caataccgtg attcaaagat
    55561 tgtacgccgt gttctgcgta gattcctttg attgccatca ttgccattag aatttgcaat
    55621 tctgtgatgt tttgaggatc tcgtgtgaat aaagcttcta attgataagg tttatctgct
    55681 ttcacgacaa cgtctaccca gtcaccaggg atatctacac gtggtaaaga atcgacaact
    55741 tcgttaactt gaacgatcac gataccatct ttgaaagctg cagcttcgat gattgttggt
    55801 gtttcttccg tattgaaacc tgtgtacaag ttgccgtctt tatctgcttt atctgcagct
    55861 actaaaacaa catttggaat caaatcgatg aataagcgtc cataaagttc caaataagta
    55921 tggatatcac ctaatttcat tgtgccgtct tcaatcattt gggcgacacg caagctttgt
    55981 gcaccagcat aagagaaatc gatcttgctt gcgattcctt tttcaaaaat attcaaatgt
    56041 tctggtcgtg agatactaga catgatcaaa tgcagatcat tcactttttc tgcatctact
    56101 ttttcaagac tttctgaaag aaaacttgct tgtttttgat tgtcgccttc taatacaact
    56161 ttgtcgccgg gagcaatcag tttttccatt acttctacga ttttttcagt ttctacatac
    56221 ttgccattga gcaagttgct gattttttcc aatcgctgcg cttttttttc gcgtttagaa
    56281 gcccagttgc ggtttggttt ttccataatt agtcatctcc atttttgata tagaaacgtg
    56341 gcgtcatcaa taccatgttg gtctagattg atcgtacgga tccctttatc tacaccaaat
    56401 tttgtatgaa tcagctttgt caaagtattg ccggggggaa attcaatcgt cagatccatt
    56461 cctaattctt ttgccaagtc catcatttgg ttccactgca cagggaaagc taggttttcc
    56521 aacaaatctt tacgaatctc ttctgcgtcc tgagtggctc ttccatccgt attttttata
    56581 tacaaacatc tagcatcaga tagatggaca gactgcatat acggcttgaa tttcttgacc
    56641 gtatcattca tcaagagaca gtgagatggc acagggactt tcagcagctt cgctgtttgc
    56701 gcttggtgcc gtttggcttc tgccaacgta acggataaac cttcgatact cccagataac
    56761 gtatgttgca tcggacaatt ttcatttgac agataaactg gatggtcagg atcaaatgtt
    56821 tttgctacaa tttcctcagc ttcgcttctc gtcacaccta ctactacgcc cattgcataa
    56881 ccgcttggat aagcttcttt cattaagact gcgcggtgat agactagttc cgctgcattc
    56941 tcaaatctca aagatcgaca agcaacagct gcacaaaaag caccaatcga atgtcctgct
    57001 gcaatatcag gctgaatacc gtttttttcc atttcatcta aataaaatac tgcttttgtc
    57061 agtaatgcta gctggataaa aaccgtatct tgatagtttt cttctttatc aactaaccga
    57121 taccccgtca cattttctaa acgttccatg tacatttcag ggatatccgc taacatccct
    57181 aacttctgac tgccttgtcc aggaaataag aaacctgtac tcatgagcct cgcctcctca
    57241 gcacttactt tttccactgc tatcttagtc aatttctgct ataataaaaa atgccaaaaa
    57301 aagaaaaaac cataacaaaa aggtatggat gggatggata attatgaaat tgcttcatct
    57361 gcagtatttt aaaaaagtat tcgaaacaaa aaatgttacc caagcagcca aagaattatt
    57421 tatttctcaa cctgccttga gccgtgcgat caaacatttg gaaaacgaat taggtgtccc
    57481 tttgttttac catagcggca gaaacattga gacgaccgtt tatgcagaag aattttatcc
    57541 atatgcagtc aagacattag acacattaga agaaggaatc aaattattgt cttcggtcaa
    57601 tgagaagatc gttacttccg tcatattgta tttagaggta gcctctgttt ctattccgaa
    57661 cttggtccga atttttatgg aaaagcatcc tgatatccaa ttaacgatca tgcaacatca
    57721 tttaccagat gatattaccg atcctgtact gtacatcact tcggagatga agcctggcat
    57781 gaccaatatt cctatcatga aagaacctgt ttatgtcgcc attcctaaga aacatcctct
    57841 tgctaaaaaa gaacatctgc aattagaaga tatccaacgc acccccatct tgatgctgtc
    57901 aaagagcaac gccttccgtc atacactgga tgcagcactt gaagcgaaaa gtatcgaatt
    57961 gacgatcagt tcgacgacag atgatcctgc gacattacgt tctattgtca agcaaggatt
    58021 agctgttagc tttttcccaa aagtttcttg gtcttacgat aaaaatgatc ctttcgtctt
    58081 gcgggaaatc aaagatttgc ccttgacacg aacaatttat ttatcttcta gcttcacaga
    58141 agatcatcct ttgatcaaaa tgattgcaaa aacgatgcgg gaattttatt taggaaaata
    58201 aaaaaaaaag agctgggaca aaattcttgt cgcagctctt ttttcgaaca aacggtgttc
    58261 cagaagtaat ttcttagaaa ataagccgaa atttcccaaa aatttgagaa gcaattttcg
    58321 taaattcctt cttatttctt gaagctaaac atttctgtca caacctcttc tctattgtcc
    58381 tagacattca ttgcgttgat ttaaaacttc catcaatcaa atctgctagt tcatctagct
    58441 tttctatttt acgtagatca atagagatcc aatgttcttt attcatgtga taggctggaa
    58501 aatacccctc ttttcctcgc aaaattccgc caagttcagg atcgaccttc aaatcaagaa
    58561 tattgatgat cctatggtca cttgcatcat catagatttt ttcttctgga acagacatca
    58621 ggaccccaaa ccattttttg cttttttgat gtctgaatac gcaataatca ggaaattttt
    58681 taaaaatata ttctggagac aaattgtatg tttcattgat gtgatcgatt agttcttttt
    58741 tcgttagcat ctgcttccat ctctttctta ttgattcgct ttattcaaag ataccacgta
    58801 agaatagaaa aatgaataca agtagttgct aatcaaatac ttcgatattt ttctttttta
    58861 tcaactctta aatgttcaat ccgcctgtgt tggtcttgct accatctact attaaaatgt
    58921 ttattattgt ttgcccacct attttttgat tttagtggta tcatttttca agttcatgat
    58981 gaaattctta tcatcagtaa atgctccaga aaaataaaca atgattgaaa aatcagttga
    59041 aacttgacca ccgtttattt tctccgtatt cttagaatag aagggagtct gattgatgta
    59101 ttcactaatg aaaaaaatca ttaccgaaaa agatattcga cgtcatgttt ctttagtaga
    59161 acaattgctg aatcatacaa agataaccgt aaatgaatta gctgaaatca tagggacaac
    59221 cgaacgaacg attttttctg accttcaatc tattcgctct cagctgcccg aaggctggga
    59281 catcatcagc gatcaagcag gaatcagttt gcaaaaccag caaaatttat taacaaatga
    59341 cctttgggag atttttttca aacaatcagt cagtgtcgaa ctgctgaaaa atctgctttt
    59401 tacaaaaaaa gttgctgtcc ctgattttct agccgaccat gggctttctt atgggacatt
    59461 gaaaagacat gtgactaaaa tcaatcagcg tttagcaagt tacgacttgc agatcgattt
    59521 gacgaaatat acagcttgta ttcttggaaa agagcgtgcg attcgtactt tttatcatcg
    59581 attgctgatt cctttcactc ataacaacta ttttttcgag gattattcga tccatgaatc
    59641 ccattatttt caatttttaa gaaacctcag tcaaactgaa ctagcggttg aaacagaaga
    59701 gattttcgga acatgttggt tctttatcaa tacgatccgt ataaaagcga attgccgatt
    59761 agattcttct atccatacca acagcacact ctcctccctg tatgactctg cattgaaaaa
    59821 attgtattta acggaaggta tttatcttaa agacactgag ctttcttttg cttctttctg
    59881 ctttctggaa agttggaatt acaacaataa ctatggacaa gaaaccgctc gctgcctgca
    59941 tcattctcca tttttagagg tcctcgaaac gtttgtagag gaactagctt cagagctgtc
    60001 tcttgatcaa ctaaaaaaaa catctctgac tgataacttg gctctcctca tattaaaata
    60061 tcatgaatcg ccgatattga ttgaagcatt ggaccgtcaa taccatcgtt ttttagaaac
    60121 atatgatcag caatatgcac acttgtatca acagaaagag aatttactga acgatctaaa
    60181 aaaagaaata gaaatcaatg aaccaaatta ctttctccaa ctcttgtctc tactgatcca
    60241 acaaacgatt ttttctatca aaccgaattt gttcaatgtc tattttattt tccaaggaga
    60301 accttcatgg aaggcgttct tgcagcagga attaaaagat tacttaggaa aacgtgtctg
    60361 cttcttacct atcgaactaa ccgatctttc cactatttct tttgaaaaaa cagatatctt
    60421 gatttccaat ttccctctag attctctaga tatccctatt gtatacattt cttctattcc
    60481 aacgaaaaat gagctcaatc gtttgactga actcactttc aaaacttttt tatagtaacg
    60541 gctagcgact gtattctcta gctgtttttt cttttttgcg aaacagtttg ttcatgatcc
    60601 ctttttctat ttctacaact aagaatagaa gaatcacatt tgctccaatc aataactgtt
    60661 gtgtcatcgt taaacttact gtaccgatca gctgctggcc gactggtaga aaaataacca
    60721 tcccttgcaa taaagcaagg atacctaatg aaagaaaaag cgctttgttt tgcaaaagac
    60781 cttggttcaa tgacggcttg gacaactctc tgcaattgat catgtaaaca gcttgagcta
    60841 agacgatatt ttgcagaaga atggtttgct gtaagctttg cccttcaaac tgcatagcca
    60901 ataaataggc aggaaccatg atcagtaggg agacatagat gatccgaaac aaatgataac
    60961 cagataggat tccttgcttt ggatctcttg gtgggcgctt catgatttct ggatctggcg
    61021 actcgaaccc tagtgcataa gataaagtga ttgtcgttac catattgacc cataaaatct
    61081 gtactgggga caaaggtagc gggcgattca gcataagtgc ccaaatcaag atcaatcctt
    61141 gagctaatgc agttggcaag aagaaattga tggttttttg caaattatcc aaaatccgcc
    61201 ggccctcttt tacggctttt gcaatcgtat ggaaattatc atctcctaaa accatatcag
    61261 ctgcctgttt gcttacttca ctacctttga ttcccatagc aattcctaca tcggatcttt
    61321 tcaaagcagg tgcatcattt acgccgtctc cggtcattcc gacgatttct ccatttttct
    61381 gcaaagcttt aacaattcgc agtttatgtt ctggtgtcgt tcgagcaaag acatcgacta
    61441 ttggcacttg ctgaatcaac tcttcatcag acataagatc caaatcgatc ccttctaaaa
    61501 ctttggcagt atgttttaag ccaatttgtt ccgcaatcgc ttttgctgtg tctttgtgat
    61561 cccctgtaat cattttcact gagatccctg cttgttggca ttcctttaca gctttgactg
    61621 cactttcttt tggcggatcg atcaatccgg cgatccctgc aaaagttaat tgggttaaag
    61681 tctcatgcgt gagttcctgt ttcgaatcta cttttttata agcaaatcct aaaacacgct
    61741 gaccttttct cgcaaattct gcagctgtct gactccatgc ttcttgttct gcagaagaaa
    61801 gagttgattt agcaaataat acttctggtg cacctttgac gtaaatcacc gctccttttt
    61861 cctccgcatg catcgttgcc atatatttgt attcagaact gaacggaatc tttgcttcta
    61921 tttttttcaa aggtagattg gctgtttctg tgtgtcgaat cagcgcaact tctgtcggat
    61981 tgccacttaa attttctgtt ttctgttctt cttttgtttt taattcctga cagtttttca
    62041 taatggacaa cacgcaggct tcttcttttg cagctacatc cacaactgtc atttcatttt
    62101 ttgtcaatgt tcctgtctta tctgaacaga tcactgtcat agaacctaat gtttctaccg
    62161 aaggaagtcc tttgatgatc gccttttctt ttgccatttc atgtactcca acggatagaa
    62221 tcatggtcaa aacagctggc aatccttcgg gtaccattga caccaccaaa gcaatcgtag
    62281 ccgaaagaag aatatgccaa tccataccat atcgaaatgt cgtaaaaata actaagaata
    62341 ggctcaaaca aagaatgcct tgaaaaattt gtttgtttaa tcggttgatt tttttaatca
    62401 gaggcgtcgt ttgttgttcg accgattgca atgcttgatt gattttaccg atttctgtcg
    62461 tgtttcctgt ttctacaacg acaccgactg cagaaccaga ttgtacaagc gttcctgaaa
    62521 aacccatatt tttctggtcg ccgatcgatt cttcttctgt caaaacggca tgactttttt
    62581 cagatgcttc tgattctccc gtcaaaatcg attcttccac taataaatca tgtgcttcga
    62641 ttaatcggat atctgctgga acgacatctc cggcacgtaa gacaatgaca tctcctttta
    62701 ccagttcttt cgcagggatt ttttcttgca tcccgtttga tagtactatc gcttcttgtc
    62761 ccatcagctg cttcaaacca ttcaatgatt cttccgcttt tctttcttgc cagtaactaa
    62821 cgagtccatt gacgatcact acgaataaaa taatacttcc ttcaatatat tcactagaag
    62881 caaatttcaa aaaagaggca atgatcaaca cgatcatcag cagatcagta aaatgctttg
    62941 ctattttttt ccatcgtttt gtctgctgcc ttgcttttat ttcattatat ccttgatcag
    63001 tcaggcgttt ctgtcgttct tcttgcgaca agccttctgt tgatgtttga tagtttttca
    63061 atacatcttc taccgtcaat ctgtaccagt tcatcctatc ccctcctgtt taattgatac
    63121 tgttattata cgcagggaag ttttttcagg cgtatcgatt atttcagggg ggtctgaaat
    63181 aattcgtttt gcaaaatgta tctgggccta actgatattt ctattgctta ttcttttata
    63241 tacaaaattt aagaatacaa accagataac caataaaaaa ggaataaagc gtgtcgtgct
    63301 ttcgaataac aagctgaaag caattgaagt aaaaaacacc aacaaagcaa gatctttata
    63361 tctttgtgta gtgttccgtt tcttttcttt aataaattga acatgtgcac acaagatcga
    63421 agaccacaca aatagaaaac aactcgttgt catgcctgcc aaaagctgaa aaaacaatcg
    63481 actatcagct acaaaaaaat gtagacaaag accggcaaaa aataacattc ctgtaattaa
    63541 aataccgtgt aaaggtacgc ctttcttaga aagcgcacaa aaccaatggg gcaaactttt
    63601 ttcttttgct gtttgatata aaatcctgct agtactgtag actgcactat ttgctgagga
    63661 caaagaagca accaaaatca ttagattgac aattgctgct ccgccttggc aatggatcac
    63721 ttgcagcata gtcactaaag gactttcttt taaatctaaa caatgccatg gaacaacaaa
    63781 taaaatcgac aaaatcgttc ctatatagaa aaagagaata cgccaaggca gacagttgat
    63841 tgcttttttc aaattctttt gcggatcact tgtctcgcca acggttaagc cgatcatttc
    63901 aattccagca aaactgaata aaaccatagg aaatgagact accattccag caaagccaaa
    63961 cggtagtaat tcatcgatct gtccaaaatt ttctttgatt ccgatagact gggtatgaaa
    64021 atctgagaca ctcaatccta tacccaataa gataaaaagg aaaatcccca agatcttgat
    64081 gattgagaaa aaagattcca attcaccaaa ccacttgact gcaacaaggt tgactgccat
    64141 taagatcatt agtgcgctac ctccgcttat caaagctggt acatgtacaa accagaaatg
    64201 gatatacaat cctattgctg ttaattccgc aagtccagct ccaagccagc aaagccaata
    64261 agtccaattg acaaaaaatg cccacttttt gcctaaataa tagtcaacga aatcactgat
    64321 agagttaaat gtactatttg ctaaaaataa atcacccatg gcccgcatca aaccataaat
    64381 agccaaaccg catatcaaat aaacgagaat aacagctggc cctgctgatg caattgcctt
    64441 tgctgaacct aaaaataatc ctgttccaat tgcgccacct aatgcgatta actggatatt
    64501 tcgattagtt agagtccgtt tcattgccgg tttttccatc aagttcacct ttttcttttt
    64561 caagcagcat ggcgacttcc tgttcccaag aaggtttctt tattttttca atacagagtg
    64621 gtactacaaa caaaatgaca ctgatggctg tgactgcaat tagattgatc catgttccag
    64681 ctttcatcgt cagtccgaca gaaaatccga ttgccgcaaa taaaacaata acgacagtcc
    64741 aaactccaag attgctcttt ttctcttttt ttccaattct aaatggtcga ttcaactttg
    64801 catgtgtata acgcaatctc aaaactgcga tccccatgat gacatagacg atacagtaga
    64861 taacagtagt tgaatttacg agcatcaaaa atgcttgatt gacgccagga atcagcaaat
    64921 aaaaaagtgc aaagattgag ataatgacag actgagtcaa aataacggat ttggatacgt
    64981 caaattgatt cgtcttccaa aaattgaatt ttggtggata cgcacctttt ctagcacttt
    65041 ctgtcactgt ctttgctggt cctgaggccc aagcagataa ttggactgct acaccaatga
    65101 agacaagtaa actaaacaga ttcacaataa gatgtggcaa accaagaatt tggcaataaa
    65161 tcgaaataga ttgcgcgata ttattcaatt ccatttgtcc ttttggtacg acatttgcta
    65221 caactaaagc attcaaaata ttgacgaaaa atataaaaat taacgcggca aatactccgc
    65281 gaatatacgt tttaactgga ttttccaatc gagtgatata cacagaggac atttcgatac
    65341 cagtaaaaat aaacatgatc ggcgcaaaat aaacaaatga ggatgcagtc gtagtatctg
    65401 gtattaattt gtcccatgaa aaactgccta atgttccggt tggtacgatc ccaactttga
    65461 tccacgcagc aaaccctaat aagagcatca tcgtcaaagg cacgtacaat cctaaccaaa
    65521 caccgacttt tccgccgatt tttgccatat caaatttcaa attcaaaaaa gtgatgatcc
    65581 aataaacgac taaaatgaca actaatgtaa atttgctgtt caaaccaagc ggcacattat
    65641 cgataatatt accaaacaac ggagcgagtg ctgaagcgac catcaccatc ccaggaaaca
    65701 tttggaccca taacagccat gagacaacga acccccattt ttttcctaga gcattcgata
    65761 cccaaagttc tggaccacct ttttggggaa acattccagc aaattcacca gagatcaatg
    65821 agattggaaa agcgtacaat aatactgcaa ccagcatgta aaaaatcatc gtccagctcg
    65881 ttgctgctac atctggaata tttcgtacac ttgctacaag ggcgcaggtc attccgataa
    65941 aggtcatggt cgtgatttgc tttgatgaac cagtatcttt catctttatt ccttctttca
    66001 atagcgacat atgtttttgt tttcttttat ttttgttcaa gaacagaaat gacaaggttt
    66061 catcgattca gtacaaaacc cagtatcacc gaaacatcca ttgaacgatc ttagtgaact
    66121 gggtttgata catgaaacga acgatccaac ttatagaacg ttttataaaa aagaatatct
    66181 tggcgaggaa tatatgaaaa acagggggaa atacaattgt tttgatgaag caaataattg
    66241 tctgttctcc atatgtctct tgttattctt tcacacaaaa atcagttcta ttggatgatt
    66301 tcgtttagtt tcttttccat tgctttggcg attttttttg tataaaaatt gaaaattcga
    66361 tcatcattca aataaggagt catgatagcc gcacgtagca gagtgatttt ctgttctttt
    66421 ttccattctt ctttcgttag acccattctt tcaacaaaac gaatcggaga atcaccgtaa
    66481 tcttcttgtg taaatgtagt atgagaagta ataaaccgtt caccataaac atcgccatcc
    66541 atataagaag accgatcgaa cattttttca tttaattcat tgatagcgtt caaatctgta
    66601 cagccttgtt ctttgaatac ccaattgacc atattaaaat caggatgatt caatggatag
    66661 acttcgattg tttttccttt aacagtaaaa gtcagatgat cgagaaattc tctgaatctt
    66721 tgagctgcct cgattgaagc cccaatcaat tgaccatatc cggtcacatt caatggcaat
    66781 acacgatgtg ctgtccagac tgcggcggcg gttgctccag cttttgatcc ttccaaaata
    66841 taagctccta acatatctgg agcctttact gatttttcaa acacatatgg cgcgaaataa
    66901 gaaatgatat ttctcatatt tttatgtttg attacgattc ctccagcagc ataagggaca
    66961 tagcccatct tatgaggatc tatcgtgaca gaatccgctt ctgaaatagc tctgaaccct
    67021 tcatatacct ctttgtcaat cgttacgcag tggtggaaaa catgatgttc ttcaaagaat
    67081 tcagctaagg aagcatacgg cacaaattct cctgcttcgt ttagaaacag tgaacgcgca
    67141 tagccgccat atgcggcatc aacatgtaaa tagaaataga tcccttcatc ttttaacctt
    67201 tctcttaatt ggacgatttt atctacactg tctacttgac cttcttctgt cgttcctacg
    67261 acagcaacca cacctagaat tgggattttt tgtccagcta attcacgaat tgttttttcc
    67321 aaagcattaa tatccattcg ataatcctct tgaactggga tagcaaccat ctgatctaag
    67381 ccgactccgc aaatatctaa cgccttcatc caagaatagt gttttgtttg agggaccagc
    67441 catttcccta atctttggat attttttccg ctacgggaag aagctgcttt gacctcatcc
    67501 atttcttcgt ccgtaaagct ttcggtcatt tccaaaattt cttcaacaga taaattcaaa
    67561 agttcccact cagacatttt tttcactttt tcaggcagca cttccttgac agctaaagga
    67621 atcgatttga tgcaacgggc gtaccataat ccttcaagat tagcaataga accatctgct
    67681 gcaatgtgtc cccatccgtc tgtcatattg aataatgaag caaaatcctg tcctacttct
    67741 gcttccattt gcgaagttgc catagaagat tccaatgcaa cattatttgg gttccacagc
    67801 atcgcatagt tgtaagctaa taaagcaggc atcaatgttt cagcattcat ttgtccccag
    67861 taacggcctg ctgaatgcca tgggatcgat cctgcgcgta atcgctgact gacttcatcc
    67921 aagacctcta tcatatggtc gcgggtggct agataatctg gagtgagctt atctccttcg
    67981 ctaattgcag gcatatctga aggaatataa ttttctctcc atccaagatg ttcatctacc
    68041 atcttgttca acaacatttt ataaactggt ccattttcag ctttgtcgcc tataaagacg
    68101 gccttgatat ccatatcttt catttttctt ccctccatat tttgttggtc aatgtcttgc
    68161 agatacatgt gtttatctgt aacacctctt cactatagcg ccaaaaatga gaattttttt
    68221 tttataacat caaatccacg tagattttta caaacaaaaa taaataaagt cggatttttc
    68281 tgataattca acgcaaaaaa gcgaacaatt tttcaataaa tcagtcttct aaaatagata
    68341 acctaaaaaa agcgaacaaa caaataaaat ga
//
LOCUS       DAJSKS010000003        56886 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJSKS010000003 DAJSKS010000000
VERSION     DAJSKS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.003"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(440..943)
                     /locus_tag="ON396_000154"
     CDS             complement(440..943)
                     /locus_tag="ON396_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR3112292.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1302..2327
                     /locus_tag="ON396_000155"
     CDS             1302..2327
                     /locus_tag="ON396_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3112293.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2650..3528
                     /locus_tag="ON396_000156"
     CDS             2650..3528
                     /locus_tag="ON396_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HCR3112294.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4004..4120
                     /locus_tag="ON396_000157"
     CDS             4004..4120
                     /locus_tag="ON396_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCR3112295.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5048..5386
                     /locus_tag="ON396_000158"
     CDS             5048..5386
                     /locus_tag="ON396_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112296.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5480..5710
                     /locus_tag="ON396_000159"
     CDS             5480..5710
                     /locus_tag="ON396_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112297.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6110..6481
                     /locus_tag="ON396_000160"
     CDS             6110..6481
                     /locus_tag="ON396_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCR3112298.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6755..7447)
                     /locus_tag="ON396_000161"
     CDS             complement(6755..7447)
                     /locus_tag="ON396_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3112299.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7450..8235)
                     /locus_tag="ON396_000162"
     CDS             complement(7450..8235)
                     /locus_tag="ON396_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3112300.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8472..9872
                     /gene="lacG"
                     /locus_tag="ON396_000163"
     CDS             8472..9872
                     /gene="lacG"
                     /locus_tag="ON396_000163"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCR3112301.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9897..10214
                     /locus_tag="ON396_000164"
     CDS             9897..10214
                     /locus_tag="ON396_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR3112302.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10227..11900
                     /locus_tag="ON396_000165"
     CDS             10227..11900
                     /locus_tag="ON396_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HCR3112303.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            11914..13545
                     /locus_tag="ON396_000166"
     CDS             11914..13545
                     /locus_tag="ON396_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HCR3112304.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(14036..15601)
                     /gene="guaA"
                     /locus_tag="ON396_000167"
     CDS             complement(14036..15601)
                     /gene="guaA"
                     /locus_tag="ON396_000167"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR3112305.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15660..16583)
                     /locus_tag="ON396_000168"
     CDS             complement(15660..16583)
                     /locus_tag="ON396_000168"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR3112306.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            16779..17384
                     /locus_tag="ON396_000169"
     CDS             16779..17384
                     /locus_tag="ON396_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3112307.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            17692..18708
                     /locus_tag="ON396_000170"
     CDS             17692..18708
                     /locus_tag="ON396_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR3112308.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            18849..20150
                     /locus_tag="ON396_000171"
     CDS             18849..20150
                     /locus_tag="ON396_000171"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCR3112309.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            20196..20858
                     /gene="deoC"
                     /locus_tag="ON396_000172"
     CDS             20196..20858
                     /gene="deoC"
                     /locus_tag="ON396_000172"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR3112310.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            20879..21277
                     /locus_tag="ON396_000173"
     CDS             20879..21277
                     /locus_tag="ON396_000173"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR3112311.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            21343..22425
                     /locus_tag="ON396_000174"
     CDS             21343..22425
                     /locus_tag="ON396_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3112312.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            22637..24199
                     /locus_tag="ON396_000175"
     CDS             22637..24199
                     /locus_tag="ON396_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3112313.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            24192..25325
                     /locus_tag="ON396_000176"
     CDS             24192..25325
                     /locus_tag="ON396_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3112314.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            25325..26281
                     /locus_tag="ON396_000177"
     CDS             25325..26281
                     /locus_tag="ON396_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3112315.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            26380..27546
                     /gene="deoB"
                     /locus_tag="ON396_000178"
     CDS             26380..27546
                     /gene="deoB"
                     /locus_tag="ON396_000178"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR3112316.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            27570..28388
                     /locus_tag="ON396_000179"
     CDS             27570..28388
                     /locus_tag="ON396_000179"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR3112317.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            28405..29112
                     /gene="deoD"
                     /locus_tag="ON396_000180"
     CDS             28405..29112
                     /gene="deoD"
                     /locus_tag="ON396_000180"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR3112318.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(29410..30096)
                     /gene="gpmA"
                     /locus_tag="ON396_000181"
     CDS             complement(29410..30096)
                     /gene="gpmA"
                     /locus_tag="ON396_000181"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR3112319.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(30268..30849)
                     /locus_tag="ON396_000182"
     CDS             complement(30268..30849)
                     /locus_tag="ON396_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3112320.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(30982..31665)
                     /gene="rpiA"
                     /locus_tag="ON396_000183"
     CDS             complement(30982..31665)
                     /gene="rpiA"
                     /locus_tag="ON396_000183"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR3112321.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            31984..32397
                     /gene="rpsL"
                     /locus_tag="ON396_000184"
     CDS             31984..32397
                     /gene="rpsL"
                     /locus_tag="ON396_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR3112322.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            32481..32951
                     /gene="rpsG"
                     /locus_tag="ON396_000185"
     CDS             32481..32951
                     /gene="rpsG"
                     /locus_tag="ON396_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR3112323.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            33045..35132
                     /gene="fusA"
                     /locus_tag="ON396_000186"
     CDS             33045..35132
                     /gene="fusA"
                     /locus_tag="ON396_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR3112324.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            35304..36491
                     /gene="tuf"
                     /locus_tag="ON396_000187"
     CDS             35304..36491
                     /gene="tuf"
                     /locus_tag="ON396_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR3112325.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            36898..37206
                     /gene="rpsJ"
                     /locus_tag="ON396_000188"
     CDS             36898..37206
                     /gene="rpsJ"
                     /locus_tag="ON396_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR3112326.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            37237..37866
                     /gene="rplC"
                     /locus_tag="ON396_000189"
     CDS             37237..37866
                     /gene="rplC"
                     /locus_tag="ON396_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR3112327.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            37894..38517
                     /gene="rplD"
                     /locus_tag="ON396_000190"
     CDS             37894..38517
                     /gene="rplD"
                     /locus_tag="ON396_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR3112328.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            38517..38807
                     /gene="rplW"
                     /locus_tag="ON396_000191"
     CDS             38517..38807
                     /gene="rplW"
                     /locus_tag="ON396_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR3112329.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            38846..39679
                     /gene="rplB"
                     /locus_tag="ON396_000192"
     CDS             38846..39679
                     /gene="rplB"
                     /locus_tag="ON396_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR3112330.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            39722..40000
                     /gene="rpsS"
                     /locus_tag="ON396_000193"
     CDS             39722..40000
                     /gene="rpsS"
                     /locus_tag="ON396_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR3112331.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            40023..40370
                     /gene="rplV"
                     /locus_tag="ON396_000194"
     CDS             40023..40370
                     /gene="rplV"
                     /locus_tag="ON396_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR3112332.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            40384..41040
                     /gene="rpsC"
                     /locus_tag="ON396_000195"
     CDS             40384..41040
                     /gene="rpsC"
                     /locus_tag="ON396_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR3112333.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            41043..41477
                     /gene="rplP"
                     /locus_tag="ON396_000196"
     CDS             41043..41477
                     /gene="rplP"
                     /locus_tag="ON396_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR3112334.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            41467..41655
                     /gene="rpmC"
                     /locus_tag="ON396_000197"
     CDS             41467..41655
                     /gene="rpmC"
                     /locus_tag="ON396_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR3112335.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            41680..41946
                     /gene="rpsQ"
                     /locus_tag="ON396_000198"
     CDS             41680..41946
                     /gene="rpsQ"
                     /locus_tag="ON396_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR3112336.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            42004..42372
                     /gene="rplN"
                     /locus_tag="ON396_000199"
     CDS             42004..42372
                     /gene="rplN"
                     /locus_tag="ON396_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR3112337.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            42409..42717
                     /gene="rplX"
                     /locus_tag="ON396_000200"
     CDS             42409..42717
                     /gene="rplX"
                     /locus_tag="ON396_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR3112338.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            42745..43284
                     /gene="rplE"
                     /locus_tag="ON396_000201"
     CDS             42745..43284
                     /gene="rplE"
                     /locus_tag="ON396_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR3112339.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            43303..43488
                     /locus_tag="ON396_000202"
     CDS             43303..43488
                     /locus_tag="ON396_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCR3112340.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            43524..43922
                     /gene="rpsH"
                     /locus_tag="ON396_000203"
     CDS             43524..43922
                     /gene="rpsH"
                     /locus_tag="ON396_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR3112341.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            43954..44490
                     /gene="rplF"
                     /locus_tag="ON396_000204"
     CDS             43954..44490
                     /gene="rplF"
                     /locus_tag="ON396_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR3112342.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            44651..45007
                     /gene="rplR"
                     /locus_tag="ON396_000205"
     CDS             44651..45007
                     /gene="rplR"
                     /locus_tag="ON396_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR3112343.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            45028..45528
                     /gene="rpsE"
                     /locus_tag="ON396_000206"
     CDS             45028..45528
                     /gene="rpsE"
                     /locus_tag="ON396_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR3112344.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            45543..45722
                     /gene="rpmD"
                     /locus_tag="ON396_000207"
     CDS             45543..45722
                     /gene="rpmD"
                     /locus_tag="ON396_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR3112345.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            45766..46206
                     /gene="rplO"
                     /locus_tag="ON396_000208"
     CDS             45766..46206
                     /gene="rplO"
                     /locus_tag="ON396_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR3112346.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            46206..47501
                     /gene="secY"
                     /locus_tag="ON396_000209"
     CDS             46206..47501
                     /gene="secY"
                     /locus_tag="ON396_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR3112347.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            47563..48210
                     /locus_tag="ON396_000210"
     CDS             47563..48210
                     /locus_tag="ON396_000210"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR3112348.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            48401..48619
                     /gene="infA"
                     /locus_tag="ON396_000211"
     CDS             48401..48619
                     /gene="infA"
                     /locus_tag="ON396_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR3112349.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            48652..48768
                     /gene="rpmJ"
                     /locus_tag="ON396_000212"
     CDS             48652..48768
                     /gene="rpmJ"
                     /locus_tag="ON396_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR3112350.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            48786..49151
                     /gene="rpsM"
                     /locus_tag="ON396_000213"
     CDS             48786..49151
                     /gene="rpsM"
                     /locus_tag="ON396_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR3112351.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            49179..49568
                     /gene="rpsK"
                     /locus_tag="ON396_000214"
     CDS             49179..49568
                     /gene="rpsK"
                     /locus_tag="ON396_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR3112352.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            49648..50586
                     /locus_tag="ON396_000215"
     CDS             49648..50586
                     /locus_tag="ON396_000215"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR3112353.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            50614..50994
                     /gene="rplQ"
                     /locus_tag="ON396_000216"
     CDS             50614..50994
                     /gene="rplQ"
                     /locus_tag="ON396_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR3112354.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            51266..52105
                     /locus_tag="ON396_000217"
     CDS             51266..52105
                     /locus_tag="ON396_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3112355.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            52081..52950
                     /locus_tag="ON396_000218"
     CDS             52081..52950
                     /locus_tag="ON396_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3112356.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            52940..53737
                     /locus_tag="ON396_000219"
     CDS             52940..53737
                     /locus_tag="ON396_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR3112357.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            53940..54686
                     /gene="truA"
                     /locus_tag="ON396_000220"
     CDS             53940..54686
                     /gene="truA"
                     /locus_tag="ON396_000220"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR3112358.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            55351..55588
                     /locus_tag="ON396_000221"
                     /pseudo
     CDS             55351..55588
                     /locus_tag="ON396_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(55732..56508)
                     /locus_tag="ON396_000222"
     CDS             complement(55732..56508)
                     /locus_tag="ON396_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3112359.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            56631..>56886
                     /locus_tag="ON396_000223"
     CDS             56631..>56886
                     /locus_tag="ON396_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR3112360.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIK"
BASE COUNT        18179 a        10157 c        12141 g        16409 t
ORIGIN      
        1 acttctaaaa actactgttt ttttcctttg aaatgccttc aaaaagtagg atcaggcaga
       61 atatactggc agaatcaagg agaagggagc ctaaataagg agaaaaacca tagaaaatat
      121 aaagtaatta cttataaaac aagcttttta tagaaaaata atatacctga gtagggtata
      181 aattaagacg gaattccttt ctagattgag atattgacta ggaagaatgt taagatttta
      241 agcctttaag ctaaaaaaaa accctttttg ttaaataagg ggaatatagc ctgtcagttt
      301 caaactgcaa gtcgtcctgc tgcaataata tagtagatta gagatgaaaa tagatttttt
      361 taaaccaatg actgaataaa aagaccgtca tagatacaca ttggaatgtg tctattacgg
      421 tcttttttta cgaacgattt tattgaagac ggacagttcg ggtcaaaaat aaaaaagcta
      481 cgatcagaaa taagaataaa atgtatccca aaataatggc tagacggtct aacttatttg
      541 tcgatagatg tttgtttaga agttgatagg cccataaacc tatcagtcct cctgttgtat
      601 tagttatcaa atcagtgatg tccgctacgc caataccgaa taggaattga atcaattctg
      661 ccaagaagct tacaaggaaa ataatcccta attttttaga aaaagaaagt ctcttgaaat
      721 tgacattcaa aagcacccca aaaggaaaaa agacaatcaa gttataagtg gtctcacgta
      781 ataccgtcgt attttcaaaa gaaaaagtag aaaaagggac gagattgaca ttgctgtaag
      841 aataagtgag aaattgtgga agattaaatg atagcttgaa taaaatcaac cataatagaa
      901 agaataaata aatccctaat aaaaattttg atacataccg catagacatc tcctccattt
      961 aaaaagtata ccatcgaaaa ccgctgaaaa atcataaaaa agtatgaagt ttaaggacca
     1021 acgtagttat cgtacgaaaa ttagaagctt ttttttgttg ggaatccggt ttctttaaca
     1081 aagctaagat agcgagttgc ttggaaaagt tgctatcttt tttctgtcaa aacgactcgg
     1141 aatgttcgtt ttttgttcta atttctccaa gttgtttact ttttgatact aagtttatga
     1201 aaaatcaaga aatagttgtt atactaggta atgctgacaa acgggattgg catttcatct
     1261 tggttcagct ttttttccaa ttactaggaa ggagggatag attgttcaag acttattttc
     1321 gtttcaataa aatacaagat aatccttttc gtatcatgaa tctgatgcta cttatcttta
     1381 ttgtattatg gttaggttac ataaatcatg attttttgat ttcaagtttt tcatcattag
     1441 ggatctttac ttttttctat tatcaaaaca taccgatgaa atatttgctg aaacgtatgt
     1501 ttctgatcgg ttgcggactg ttggttgctt tcgttttggg gatcttatcg acttatgttt
     1561 tatggcttga accatttgcc gtagctttgg ttgctttttc cagcagattc gtgttgcgat
     1621 tattccatat ttctaaacca ggtggcttgt tttttgcgat gctttctgcc atgggaacga
     1681 gcatgcagct gccaatcgct caattaccga tcgtctcact ttattttttt atgggtgtcg
     1741 tttttgccct gattgcggca gtgattacaa aattactgga ttcacggcct gagcagacaa
     1801 ttgaaaaagc aactttaaaa gaaagatttc acgaagagcc tttagtgatc attgattctg
     1861 ttttctacag cgctgcctta tttctttccg tttatgtcag tcacggcttg aatctgcata
     1921 atccttattg gttgaccttg tcatgtgcat cgattttgct agcagaaaac ttggatgcga
     1981 tgaagcatcg acaagtccaa tatctgattg gatcgatggg tgggttatgt gtgtcggcat
     2041 tcttgtcatt tgtgccgttt acgcagttgc agaccatttt tctgatcact tttctatatg
     2101 gaatcgcaca atttctagtt gctagaaatt atgcagttgc taatattttt cttaatccga
     2161 tggcgttgat gctatctaca cttattcgtg gagcatactt gatttctttg atcgaatatc
     2221 gttttttagg gatcgtcata ggaagtttca tcggtttagg tgttgcgtgg gtaatgacgg
     2281 ttggtttgca acattattta acggttgttc gtcaaaaatt agaatagaag gacaaatata
     2341 tgtgccaaaa gaggttatga caagattttt gtcacaacct ctaattcaag gtgttcaaaa
     2401 gccagattct cagcattaag tcaaattcac acaaaaatgt ggaaaggaac tttcaaaaaa
     2461 attttatatt tattctatca aaaaattttg tttatgatgg taactgaata aactttcctg
     2521 agatggtcaa tgctttcctt gaaagtctta cagatattct ttcttcatag aaagtttaag
     2581 ttttatagtt agtgtacaat aaaagtatgt ttataccata tggtgtccgg ataatcagtc
     2641 atttaaacaa tgaggttcaa agatatgaaa aggggaaatt tgatgaagaa aataagcaat
     2701 gaaatccaaa ttctggcaga aactttaact cttcccaaag tcgttataga gcaaatgatt
     2761 gtacaagcaa aaaacatcct tgatgaggtg tttgagcaat atagtcatga actaatggat
     2821 cctaataaag caaaggcgac tgcaaagaaa atagacaaag tctatgaaga ggaacctttt
     2881 gcagcattag ctatttatct atatgcagct tctcaaagtt ggaaaaaaat ttatcaacca
     2941 ttgaagattc caagaactat ctatttggca acaatgaatg cttttactag atttattcat
     3001 gaacatttcc aagtaacagg aagctatcgc tttgaccgtg gtttttggat ttggcattac
     3061 actagtggct tgatctttcg gattggtgaa ttggagtacg aaatgacata ttttccgaaa
     3121 gggtacacgg atctaagatt agaaggaaag cagtgccttt ctcttcatat tccttccgat
     3181 gcagatttat ctcagaataa gataagagat agttacgaaa gtgccattcg tttttttgaa
     3241 ctttattttc ctgattacca ttaccaagtg atgtacacag atacttggtt actatcaccc
     3301 aatcttacta aatggttgaa aaaagagtca aaaatctgtc tttttgcagc cgattatcga
     3361 ttattatcag ttgatgaaca ggacgatagt ggagtccctt ggatctttgg acgagtagat
     3421 gcccaaatac atgattatcc agaaagtacg agtttacagc gacaagcaaa agagcaattg
     3481 cttgcaggcg agcacattgg ctctggctta ggtattctta cttcataagt ttagtacttc
     3541 gaaatagttt aaaagagcgt gagacaaaag taaagaacac ttttgtctca cgctctaaac
     3601 acgtataaac ggcggaagca gaagcaactc cttcggaaat aagctgaaat tcacaaaaat
     3661 ttgaaaagca attttcgtga atttcttctt atttcttgga gttaaacgct tctgtcccag
     3721 cctctttttt agtgttctat cgaacacaca gtacgttcaa taggtaaact agctataaca
     3781 atagatatta aagttaacgg acaagagggc cttttttagg tagctaaaac gtaataatga
     3841 aaatttattt ccagagatag gagtcaatca gctagtctga tcgactgttt taacaagttg
     3901 actttcattt taacttttga taccatttaa aacacaaatc gtaattgttt cgatttgtgt
     3961 tttttagcat gactacaatc aaagaagaaa gtgggtcgtc catatggaaa aaacagatat
     4021 ctcaagtgca taccgccgct tgaaaagccc aaatatcaaa acacgaaaac gtgcgctgaa
     4081 gatcatcaaa gatgtgaaac ggaatagtgg gaaaaggtaa gaagaaggaa acttaattaa
     4141 aaaaatagcc gaaaaacgtt tttcggctat tttcaataat cggtttttgt gtaaggtttc
     4201 cgaaagaact gctgttgatt ttccattttt aacatatgat aaattggtag aatctttctt
     4261 ttcaaattga taccattccc taattccagt tatatagtta actacagaac cacaaagcac
     4321 gtacgtttat aaaagtatta tctgtagtta tttttcaatt ccctttcaac ctcttatttt
     4381 gaccagtagt attgatcact ttacctttta acacctttgt tcgtttttga aacattttta
     4441 gtcaagaatg gaaacgtaac tacataatca aaggttttgc gaaatatttc atgaaatatt
     4501 tcatgtagaa gcgagcaata cggttgcaaa taaccattat cgtttaataa gcagttacga
     4561 aagctcccaa tatctctaac gttcagctca ctccatttct tttcttcaaa aaaatcagtg
     4621 attcgtatct ctttctattt gtcgaaaaag ttaactttaa aaagtaacgc cgaaaaaata
     4681 tgcaaaaaaa taaaaaagca gttactattg ctagaataac atcaaggaat aataagtttt
     4741 ggttttcaga taatagtcga taagttgtgt attggaattt tagatacagt aataaagtat
     4801 ttttcatgaa attttgtgag aatcactatt acaagtatct ttaataaaat taatggttaa
     4861 ggaggaactt aaagtagaat gtaaatgaat agttgtagtc agtcgattgt gatactagac
     4921 tcaaagttgc aggaataagt ttttcaattt gtgattggaa gataaaaaat gaaaaagaag
     4981 ggcttttttt tatactttgt tgattgtagt gtttattttc cctctgttta gccaagtaac
     5041 attttctatg actcaaccag tttcaagtga ggaacatgat aacctttttc gtgctacaga
     5101 agagtttaag aaatatcagg aaaatattgt tccggagact ccattagcta gtgagccaat
     5161 tactgatatt gatggaaacc aaataggatg gtatatacag tacgaagtta tggtggtaaa
     5221 taatgactca atagtaaaaa atacagtagg cttagagcta acaccgattg aaaaggtgag
     5281 ttcattgtta acgtatattt atactgatga aagtgacgag actgagatca ttttaacgga
     5341 ttactcaaaa attgctactg ataaaaagta tatattgtgg atgtagacac tagtgaaata
     5401 ctttatgata tagaaattga tgaaaattta ctagatgtag cacaagaaat cgatcatcaa
     5461 aagcaggaag ctattcgata tgctggtaga aaagggaaaa gaaaatattt attacgtgaa
     5521 tgtagcaaaa gttagagaag atgaaaatga gtggaaagaa ttcaagagtc gctactcaat
     5581 taactcgact ccgacattta cagtttacag agaaggatcg atagaaaaaa ctgttttttg
     5641 gactaaagaa agtggaatgt ctttagctga agtagaagag tttttggatt atgttagtat
     5701 gcaacagtaa gggagttctt attatgaaaa attttgacac atattatgtt attgtttttt
     5761 cgatatcttt gctgttaata ttactaactt taatgcagta taaagagtat ccagagtatt
     5821 ttattttaac ttttgtatat ttagtaagta gctacacact tctatataaa ttcagaagtt
     5881 cttctagagt ttctaaagtt tgtttcttaa ttagcttatt gattctataa tatttttgat
     5941 ttatatagga aattaaaaat attatagaat caacactcag agaacgggtt ggtttttaat
     6001 agatttaata agtactgata atgaaatttc tcctggttgg gaaaacgaag ttggttaccc
     6061 tgtagaagaa ttttttcaag attactttgc ggagtatgcc gataggataa tgagagatta
     6121 cgtaacagat aaaactcaag aagtcatcaa tcaatcccag tcattaaatg aattatctga
     6181 taaggcgaaa gaagaagcag ttgccattat ttcttcaggg attagtgact atattgatat
     6241 gagtggtgaa atcaattatg gcgataaaga caaaattgta cgcatgataa aagcttttat
     6301 caaaactttt gatgtgaagg cagatcttaa gtttaatgat acttacctag taggaaaatt
     6361 aattaatatc cttgggacag agaaagatac ggaaacgttt atttcctctt tgtctatgtt
     6421 gtttacagat aagcattttt caactcagct tggtggagag gaactagtgg aaatctttta
     6481 accattacgc ccagcattaa taaaagtata tcaaaaaagt actcctgatg atttttaaga
     6541 ttggtttgag aaataatgtg aaaacactat ttttaatgtt aattatagat agagatgaac
     6601 gcgtcatatg gttaaaagaa tgaaaaagtc aaagatacac tgctatattt cactaaggat
     6661 atagcagtgt atctttcatg tcgtttcact ggcagttttt tataaaagga ggtgtgcatt
     6721 tcagttgctt ttctttggta gttagttgaa tcgtttaaga attaacggat gtggcagaca
     6781 ggtgctctgt ccaaaaacca gtaaaatctt tgatggttcc atctttattt agctgtgcct
     6841 gttcaaatac tgttttgttt ttaccgaatg gatcaattgc aataattgtt ccgatccccg
     6901 cacgtttggc agcaagtaat cctgagtaag catcttcgat aacaagacaa tcagccggag
     6961 ctaagctcag tttgtctgcc gctttaagaa aaatatccgg ttgcggtttc ccagggaaag
     7021 agccgtcgtc atagacaatc ttgtcccagt caaaccagcg ggctaaatcg aaaatatcaa
     7081 aataaaaagc aacattctct ttgacagtag ccgtggcaat cgttaatgga atctgttggt
     7141 cagtcagctg gtcaagagtc gcttcaagcc cttttgtcaa ctgaagctcg ttttcatttt
     7201 gcaagcaaag catacgataa tagctttctt tttcttctga caatacggcg atctcagagg
     7261 cggtcaaatg ctctgaaatg aaatgtttca aaatctccgt attcgtccga ccatgaatat
     7321 tttttaaaat atcctcgtct gataaaccat cttttgtatg atcatgtatc atatgcagcc
     7381 aagcagcttc atgcagatga gagtccaaaa acattgttcc gttgaaatca aatataatcc
     7441 ctttcatagt tacgctccta tagtggtatt ttcggttgaa tcagcaattt gcagatcgac
     7501 atattgtgca tagtgctctt gaacctcccg cgagatctgg cgatccgtaa tgagcagatc
     7561 gaaatcatgc aaattataaa acacataaaa atcatctcga ttgaattttt tattatcaac
     7621 gagtagatat ttcttacggg aattatttag agcgatctgc tgtgcttctc cttcttcggt
     7681 actgtaagta gagatcgctt cgttgtgaac gccatttgaa ctgataaatg cttttgtaaa
     7741 ctttagtctt cttaaattgt cgttggtgat cggtccgaca aatgcgccgg tatttttccg
     7801 gaaatcaccg ccaatcagta gaatttctgg tccatctgct tgagcaagaa tatcaaaaac
     7861 tggataatta tttgtaatga tccgaatttt cttgttcagt aaatgcgtcg ccaatagttc
     7921 gattgttgta cctggtccta agaaaatcgt ttctccctcg tgaatcaagg aggctgctgt
     7981 ggcagcaatt tctttttttt cttgaatctg cagagtggct ttttcagtgt gagaaagttc
     8041 ttgatctaaa gtgtaggaaa gactttgagc ccctccatgg acacgcagca gcttgcccga
     8101 tttttctaat tcatctaaat ctctgcgaac ggtcatatca gaaacatcta attgattgat
     8161 gatttcatta acggtaagga ttcctttttt attgaccatt tctacgatag taagcaatct
     8221 ttcttttttc aacattgtct tcacctctaa tttaaggata atctatcata taaacggaca
     8281 acagtcaatc taattcgaac aaaagtaaac atttagggtg gtttttatgt ttgttacaat
     8341 agattttatt gctgttttct ggtttttatg attatttgtg tttgtttttg ttgacaaatg
     8401 ttttggatgc gtttacaatg caatcagtta gaaagagcga aaactgatta tagatttgag
     8461 gagagatgta catgcttaaa ttaccagaag attttatttt tggcggagcc acagcagcct
     8521 atcaggttga aggagccact aaggaaggtg gcaaaggcgc tgttgcttgg gatgactttt
     8581 tagaggaaca agggcgattc agtcccgatc cggcaagtga tttttatcat caatatgcaa
     8641 aggacattga attgtgtgaa cgttttggtg taaatggtct aagattatcc attgcttgga
     8701 gccgcatttt tcctgatgga gctgggaaac caaatccaga aggaatcgct ttttatcatc
     8761 gtgtgttcga agaatgtaag aaaagaaacg taacgccgtt tgtgacgttg catcattttg
     8821 atacccctaa acgtttgttt gaccatggag attttttgaa tcgagaaaca atagaagcat
     8881 ttgtttccta tgccattttt tgttttcatg aattcaaaga agtcaaggtg tggagtacct
     8941 ttaatgaaat ctatccagtt gcaaccaatc aatatttgtt aggtgttttc ccaccaggaa
     9001 tcaaatatga ttttacaaag atcgtcgcct gtcttcataa tatgatggtt gctcatgccc
     9061 gtgtagtgaa ctatttcaaa gaaaacgaac ttccgggtga aattggtgta gttcactcat
     9121 tagagacgaa atatgcggca acagacgcgc cggaagataa gcatgctgcc tttttagacg
     9181 atgcattgtc gatccgtttc ttattagatg ctacctattt aggctattat tcaactgaaa
     9241 ccttgactgc tcttgatgag atatgtgaag caaatcaggc cagctatcat tttcctgaag
     9301 aagatttcgt tgaattgaaa aaagcatcca cgcgtaatga ctacttaggg atcaatcact
     9361 accaatgcca ttttgtcaaa gcttacgatg gagaaaatgc aatccatcat aacggtacgg
     9421 gtgaaaaagg gacctctgtt tataaagtga agggaatcgg agaacgtatt tataaagaag
     9481 gaattcctcg aactgattgg gactggctga tttatccaga aggtctatat gacttgttgc
     9541 tgcgaattaa aagcgattac ccacattaca acaaaatcta tatcactgag aacgggatgg
     9601 ggtataaaga tcagtttgaa gatggcatta ttatggatca gccgagaatc gactatttga
     9661 gagtctattt ggaatcctta agcaaggcga taacagccgg ggtaaatgtg aaggggtatt
     9721 ttttgtggtc gcttatggat cttttctcat ggacaaatgg gtataacaaa aggtatggct
     9781 tgttttatgt tgattttgaa acccagaagc gttacccaaa agaatcagct tactggtaca
     9841 agttggtcag tgaaaccaaa acgattattt aataagtgat aatggaggaa gaaaagatga
     9901 ataaagagga attacaaatg cttggttttg agatcgttgc atattcaggc gacgctcgca
     9961 gtacattatt gaaattattg aaagaagtaa gacaaggaaa ctttgaacat gttgattcgg
    10021 cgttgaaaga agccgacgaa aatttaaccc ttgcacataa ttcgcaaaca aaaattttag
    10081 cggaagaagc atctgggaaa gagatggaga tgggattcat ttttatccat gggcaggatc
    10141 atttaatgac cactttattg ctgagagatc tgattcaaga ctttattgta ctgtacagac
    10201 agaatcaagg ataggagaag aacgctatgg atggaatcat taaacaaatc gaaaaaggga
    10261 aaccattttt tgaaaaaatc tcacgtaata gttatttagg agccgttcgt gacggatttt
    10321 taacagcgat gccagctatt ttattttcaa gcattttcat tttgattgct gcggttcctg
    10381 agatttttgg ttaccaatgg cctgaatctg tctccacatg gctatggaaa gtctataatt
    10441 attcgatggg tgttgtaggg ttactcgttt cagcaaccac tgctcgctgc ttggctgatt
    10501 cgatgaatcg tcgcttaccg aaaaacaaag taatcaacac cacctctgtg atgctggcct
    10561 ctgtggtaag tttcctgttg ttaagcgtct ctccgattga aggtggattt tcaacggaat
    10621 atatggggac aaagggacta ctggcttcct ttgtcgctgc atttttaaca gttaatttat
    10681 ataagttttg tgtgctgaaa gatattacca ttaaaatgcc taaagaagtt cctggaacga
    10741 tttcgcaaac cttccgagat atttttcctt tctctttttc agtatttgcg gccgttttaa
    10801 ttgatttagg cattcgacaa atcttccaaa tctcatttgc agaagcaatc attacggtgc
    10861 tgcagccgct atttacagca gctgacgggt acttaggaat cgctattatc tggggagcga
    10921 tggcgctgtt ttggtttgtc ggtgttcatg gaccgtcgat cgtggagccg gcaattgcgg
    10981 caattatcta tgccaatgtg gaaaccaatc tgcaattgtt caattccggt gaacaagctt
    11041 ctaaagtact gacggtgggt ttagggaatt ttgtaggaac tatgggagga actggggcaa
    11101 cattggttgt tccttttatc ttcctactct ttgctaagtc taagcagttg aaggcagttg
    11161 ggaaagcttc ctttattcca gtaagtttcg ctgtcaacga accattgcta tttgcggcac
    11221 ccatgatttt gaacccttat ttttttattc catttttgtt aacacctgtt gtaaacgttt
    11281 gcctgttcaa gttttttgtc gatgtattgg gcatgaacag ctttatgtac gttttgcctt
    11341 gggcaactcc agcaccgatc ggtttggttc taggaacggg catgggtatc ttagcttttg
    11401 ttctagcagc ggttctaatc gtcgttgact tcctgatcta tctaccattt tgcaaagcat
    11461 acgatgctgt cttagtagag aaagaagcac aaaaagcaga ggtgatcgca accgaggaag
    11521 gtgcttctgc cgatgtacgt gaggaagtac ctgtggcagc gagtcaagtt atcagcgatg
    11581 caaataaaga aatcaatgtg ttggtacttt gtgctggcgc agggacaagt gcgatgttag
    11641 ctaatgcttt aaaaaaaggt gcatctgaat cgaaagcacc gatcagtgca atggcgggtg
    11701 cttatggttc tcattacgaa atgctgcagg attttgatat ggtggtctta gcaccgcaag
    11761 tcaattctta ctatgatgat atcaaagaag acaccgatcg cttaggaatc aagctggttg
    11821 cgacaaaagg agcagaatat atcggattaa cgagagatcc tgaaaaagct gttcagttta
    11881 ttctgaacca atttcaataa aggaggagcc ataatgaaaa tgggaatttg gttgcgtaaa
    11941 gctgttttat ttgcagctgt gttaggggct ttgggggctg tttcaaaaac tgttgaagca
    12001 tctgccagcg aatttattcg cggagcagat atttcgatcc tgtcagatat ggaaaaatct
    12061 ggtgcaaaat attatgaaga cgatatttca aaagatgcct tagagatttt aaaaaataat
    12121 ggtgtcaact atgttcgact gcgtttgtgg caggatcctt ataatagtaa tggagaaagt
    12181 tatggagcag ggacaaatga tctggaaacg acgattgacc ttgcaaaacg agcaaaaaat
    12241 ctgggactta aggtgctgct ggattttcac tacagtgatt tttgggttga tcctgggaaa
    12301 cagaatcttc ctaaagcatg gcaaggtctg acctttgaag aaatgaatac ggcattgtac
    12361 gactatacaa agaacgttct gtcggaaatg aagcagctgg acgtttatcc tgatatggtt
    12421 cagatcggca atgaactgaa tagcggaatg ctctggcctt atggcaagtc ttggggagaa
    12481 ggcgggggag aatttgatcg attggctgct tttttgaagt ctggtatcca agctgttcga
    12541 gacactcaac ctaaaacaac tccagtaatg ctccacttag ctgatggtgg cgatacagga
    12601 gcatttactt ggtggtttga tgaaatcacg agtcgaggtg tgtcatttga tttaattgga
    12661 gtgtcgtatt atccgtattg gcacggttca atgaatgaac ttcaaaccaa tatgaatgcg
    12721 atcagccaac ggtatggtaa agaagtgatc gtagttgaaa cagcttatgg gcatacaaca
    12781 gcgaatgccg atactatgcc aaatgctttt ggtgaagccg aagcagctgc ggggggatat
    12841 gaaccaacac cgacaggaca agcagagtat ttgctggatc ttgccgatcg gattcaggca
    12901 gtaccgaata atcgcggggc gggcttcttc tattgggaac ctctttggta caacggaaat
    12961 gtgagttggg caacacaagc aggtatgaat tatctcagtg ttcaatccac tatgggaaac
    13021 gaatgggaca accaagcgat gtttgatttt gctggaaacg ccttgccaag cttgcgtgcg
    13081 tttaaacaag caggggcgca aataaatcta gttaaaaatg caagtttcga aagcgatgga
    13141 ccaacgacca gtccttccaa ttggcaaacc tggtttcaac aaggaaccgc taagggtacg
    13201 atcaaaacgg aagcgggcac tttttttggg gactacaaac taactttttg gcaggatcaa
    13261 gcatacgagg catcagctta tcaaaatgtt agtggcataa gcaaaggaac ttacacattg
    13321 agtgcctatg taatgtctgg gggagagttt gatactaatc agttatatat caaaaattat
    13381 ggtggagcgg aactaaatac tgcggttgtt aaaacaagtg aatggacaaa aattacaatc
    13441 aaagacattc caattacaac aggtgtttgt gaaattgggg tttatagcaa ggcttcagga
    13501 aatgcttgga cgaacgttga ttttgtttca ttggtgaaac aatagactgc ggatcgtttc
    13561 gattcaagat cggaggttcc cttcagccac atcgttagcg tagaaggcag gaatcgaacc
    13621 tgcacctcgc ctttgccaaa aataaccaga aacgatttcc agtcattttt accaaaacga
    13681 gtaaagcacc agacttcatc tacactttgt attatgctaa agagagttca acgatttcgt
    13741 aagaagcaat gaaaaaattg caaaaagttg tgcagataac ataaagtttg tttaagaaag
    13801 ggtaacctta gaattccgtc gtgaatatga tttgagcact tagatggata gaaaaaagtt
    13861 ctcggaaaac agttcgtttt tcgagaactt ctttttatga taagaatgaa acaaattaac
    13921 ttatattata gtctcttttg gtagggaacc atcttatttt taatgaaaat caagcatttt
    13981 agctattgaa gaatgttgat ttatagggat tccccctcca tattatgttg gctctttatt
    14041 cccactccac tgttgctggt ggtttagaag tgatgtcata cacgatacgg ttgacgtggg
    14101 ctacttcgtt tacgatacgt acagagattt tttgcaagac ttcccatggg atacgggcaa
    14161 agtcagcggt cattccgtcg attgaagtta cagcacggat accgactgtg tagtcgtaag
    14221 tacgtccgtc acccataaca ccgacagagc ggatccctgg aagaacagtg aaatattgcc
    14281 agatatcacg gtcaagtcca gcagctgcga tttcttcacg caagatagca tcagattcgc
    14341 gtacgatttc tagcttgtct tcagtgattt cgccaagaac acgaatacca agaccaggac
    14401 cagggaatgg ctgacgccaa acgattgaat caggcatgcc taactgagtc cctaattcac
    14461 ggacttcgtc tttgaataat gtgtttaatg gttcgatcaa ttcaaattgc atatcttctg
    14521 gcagtccacc tacattatgg tgagatttga tcgtctgtgc tgtttctgta ccagattcga
    14581 taacgtcagt atacaacgtt ccttgtgcta ggaaagagac gccttcttca ccagcaagtt
    14641 ttgttgcttc gtcatcaaaa acatatacga attcattccc aatgatttta cgtttttgtt
    14701 ctgggtcaga tacacctttc aacttgtcta agaagcgatc tcttgcattg actttgatga
    14761 tgttcaagcc gaattttccg cctagacttt ccattacttg ttccgcttcg cctttgcgta
    14821 gcagaccgtg gtcaacaaag atagatgtca attggtcacc gatcgctttt tgtaaaagaa
    14881 ccccaacgac actagaatca acgccacctg aaagaccaag caagactttt ttgtcaccaa
    14941 cttgttcacg gattttttct acttgcatat cgataaagtt atccattgtc cagttacctt
    15001 cacagtgaca aacatcaaag gcaaagtggc gcagcaattc gattccgtat tcggaatgac
    15061 gtacttctgc atggaattgg ataccataaa aattacgtgt tgtgtcttgg atagaagcaa
    15121 tcggacaatc tttgcttgtt ccgactgttt caaagccttc aggaacttta gttaccaagt
    15181 caccatggct catccatact gtttgttgtt ttggtgtttc tttaaacaag acagcttgat
    15241 cgcttgttac ttctaacaag gctttcccat attcgcggtt tttagcaggc tctacggaac
    15301 cgccaagttt gtacgtgatc aattgcatac catagcaaat ccctaataca ggaataccta
    15361 aattgaagat ctcaggatcg atcccaaatg cattctcatc atatacactg tttggtccgc
    15421 cagaaagaat gattcccttt ggattcatcg cttttacttc ttctgcagta atgcggtggc
    15481 tcaataattc ggaaaagact ccaagatcac gaatacgtct tgtgatcaat tggttatact
    15541 gactaccgta gtctaatacg ataatctttt caacgttcgt catatcggca acgtttgtca
    15601 aatttattac ccctatccct ttaaatttta tctcaaaata gtcaaactat tttaaaatgc
    15661 taatattttc gcatgaaaat cgaatcgatg atatgatttt cagttttatg gagaattata
    15721 tcggctcttc ctcgtgtagg caagatatat tcctgtaaat ttttcaaatt cacatttttc
    15781 catacatctt ttgccatttt aaaggcttct tcacgatctc cgatagcaaa acgataataa
    15841 taattctctg gattttggaa agccgtatcc agtaacgcac caaaacgatc caaataccat
    15901 ttttcgatca gcacgggatc ggcatcgaca tagacagaaa aatcaaaaaa gtcgctgacg
    15961 tagatttgct gattcgcagg taattgaagc gtattgatcc cctcaacgat caagatatca
    16021 ggctgctgga tcaattcgta cttgtctgga atgatatcat acacatcatg tgagtaaaca
    16081 ggtactttta tctcttcctg accagatttt acacggttca agaaatcgat cagcatctcc
    16141 atatcataac tttcaggaaa tcctttacgg tccatgattc cacgctcttt taatatacga
    16201 tttggataaa gaaatccgtc agtcgtgatc agctgaacgt ttcttcgttt gaaggtacga
    16261 gacaaaatcg tctgcagcag tctggctgtt gtactttttc caacggctac acttccggcg
    16321 atcccgataa taaaaggcgg tacttctacg tatttatgaa gaaataaacc tttactcaag
    16381 aacaaagatt caaattcttt catataaaga taaatcagat gagcaagagg tacatagata
    16441 tctcgaacat cctgcatgga aatgcggtca ttcaaacttt tgatattatc tagttcatct
    16501 tgtgtcaaag gtgcttgccc attacgataa aattctcgcc attcttctct tgccacacgg
    16561 tagtagttca ttggatcatt catattagcc tcctgcgttc tcccatcaat ggaactattt
    16621 taacacaaaa taaacgcaaa ccccaccact taagagagat tgccgatctt tttgtataat
    16681 ctttataata gaaagaaacg gagtcttatt cttgacttgc gcacggatac ataaaaggat
    16741 agactgttgt catgaaagaa atgagaagga tggaagacat gacaaatcat tactattctg
    16801 aacgcccaga tacggcgcac gattttgaac aatgggcttt tgagttaaaa ggcagaacct
    16861 ttcaatttgt aacagattct ggtgtatttt cacgtgaaac agtggattat ggttcacgtg
    16921 tcctgattga tgctttcgat tgggaagagt tacctgatga aggaaagatc ttagatgtgg
    16981 gatgcgggta tggaccgatt gggttagctt tggcatttgc gacacagcgg tttgttgaaa
    17041 tggtcgacat taatgaacga gcggtaagtc tagcgcaagg aaacgcaaag cgtaaccaaa
    17101 tcgaacatgt tgatatccat caatcgaata tctatgaagc agttcatgaa gaaacctatg
    17161 cagctatcgt aagcaatccg ccgatccgtg ctggaaaaaa agttgtacat gaaattttga
    17221 caggcgcata tcctcggttg aagaagggcg gtacactgac aattgtgatc cagaagaaac
    17281 aaggagcgcc gagtgcgcaa aagaaaatgg aagaaacatt tggtaatgca gaaatcgtca
    17341 caaaagataa aggatattat atcatcaaaa gtgtgaaaga ataactagct gaaaaatagt
    17401 ggagcgacga aagagtggaa ctgggcgtgc atgtacgggt acgtatcagt tctttttttg
    17461 ttgagaagtt tttaaaaaca aagaactatt ttttcttcaa aaagaaatag taggaacaga
    17521 gtcgttgttt tttacgataa aataaataag atacagaact tgtatagaag gctgccaagt
    17581 cggtagataa aggaggtttt tatcaaaaaa catactttcc ttttttctct attcttttct
    17641 tgggggtttt ggtacactga tagggaattt agatagaagg gagccaatag gatgaagctg
    17701 accatcagag atatcgcaga aatggcaggc gtatcagtaa caactgtctc acagattttg
    17761 aataacaaag gaagcagatt tagtgataag acaagaaaaa aagtgttgtc gatcgtgaat
    17821 gaacatcatt ataagcccga ttatttcgct tcaaatgtga tcaatcgaca ttcaaagacc
    17881 atcggaatga tcgtccctga tgtcactgat ttcttcttct ccaaactgat cgaaggtgta
    17941 gagagctatt tgaatccttt gggctatgtg atcatgttat gcaattctcg tcatagccaa
    18001 gaaaatgaga tcaagtattt acaagagctt tcccaccgct cagtggacgg aatcttgctg
    18061 gccacaccaa atattttacc tgagaaatat gcattagaca gcggttttta tcaaaaaatg
    18121 ccaatcattt taatcgatcg cggattgaat cgtcgggata acggacggct tatcgtaaag
    18181 gaatatgagg gcgcctatca agctgtttcg ctgtttattg aaaatggcca tacgaagatc
    18241 ggcatgctga aggaaaccac tggatactat caattagaag aacgtttcaa cggatatcgt
    18301 catgcgctga aagatcatgg cctgcctttt aatgggaaat ttgttgaaca tggagagttg
    18361 actgtccaag gtggatatac tgcatcaaaa aaattactca aacataaaga tatcacggct
    18421 attttttgcg ggaatgatgc catggcgatc ggatgctatc aggcgatcga tgaattgggc
    18481 aaaaaaatcc cagaagatat ctcagtcata ggattcgatg gattaaagct gtcagaatac
    18541 atgattccgc aattgacgac agtgcagcag ccaagttttg atattggatt ttatgctgca
    18601 cgtttcctga tcgatacgat cgaatttcct caaagaaaag ttccgaacaa agtgttcgaa
    18661 acaaaattaa ttattcgtga gagcgtaaaa gtattgacaa agggctaact cggcggtata
    18721 ttgcttatgt aagcatttac aaaggctagt tagtgctttt tttattttta gtaaaccagt
    18781 ttactaaagt ggtttacctt tcctaaaaat aatgtataat accgaatgaa gacaaggagg
    18841 atcaggcaat gcgaatggtg gatctgattg aaaaaaagag ggatggaaac gagctgtcga
    18901 aagaggaaat cgagtacatc gtcacgaact atacgaatgg aaagattcca gattatcaag
    18961 tcagtgcatt attgatggca attttttatc aagacatgac aaatgaagaa atcacgaact
    19021 taacattggc gattgctaat tctggtgatg tcatcgattt gagttctctt gaaggaataa
    19081 aggtagataa gcattcgaca ggtggcgttg gtgacacgac cacattgatt ttagcccccc
    19141 tagtagctag tgtcggagtg acagtggcta aaatgtctgg acgcggtcta ggttacacag
    19201 gtggtacact agacaagcta gaagcaattc ccggatttca gatcgagctt tcggatgaag
    19261 aatttgtacg cattgttaat gaaagtaaag tagcggtaat tggtcaatca ggaaacttgg
    19321 caccggcgga caaaaaattg tatgccttgc gtgacgtcac agcgaccgtt gattctcttc
    19381 cgctgattgc cagttcgatt atgagtaaaa aaatcgcggc aggagcagat gcaatcgttt
    19441 tggatgtgac cactggagac ggtgctttca tgaagaacat cgaagatgca agacgtctgg
    19501 caaagaccat gacgagcatt gggaaactgg ctaaccgaga aacagtggcg gttatctcag
    19561 atatgtcaga acctcttggc gaagctatcg gaaatagtct agaagttgtc gaggcaatcg
    19621 aaacattaca aggaaatggt ccagaagatc tagtagaaat gtgctacgcc ttaggaagcc
    19681 aaatggttgt tcttgctggc aaagctaaga caaccgatga agcaagaacg ttgcttcaag
    19741 aagctctgga atcaggtaaa gcgttagcaa aattcaaaga aatgatccaa aatcaagggg
    19801 gagacccagc gatcgtggaa catccagaac gaattttgac tgcacgctac acaatggagc
    19861 ttcctgctaa acaaagcgga gttgtgtcaa aaatcgtggc aaatgaatta ggaattgctg
    19921 caatgatgct aggtgctggg cgtaaaacca aagaagaaga tattgatcac gctgttggat
    19981 tgaaactgca taaaaaaatt ggtgacactg tgacaaaagg tgagtcacta ttgacgatct
    20041 atagtaacga cgaagaaatc acttctgtca ttgaattatt atataaaaat attgaaattg
    20101 gtgaatcggc aatgaagcca acgttgattc atgacatcat cacagaataa aggaaaaaag
    20161 cggactttac cgttttaaag aaaaggggaa aagaaatgga actaaaccga atgatcgacc
    20221 atactatttt gaaagcagac gcatctaaag aagctgtgat gcaaattatc gaagaagcaa
    20281 aaaaatacca tttttattct gtctgcatca atccagcatg ggttgcgcta gcgaaagaac
    20341 aattacaagg tgagccagta gccgtatgta cagtaatcgg gtttcctcta ggagcaaaca
    20401 cacccgagac aaaagcattt gaaacaacag atgccatcaa taacggtgca gatgaagtcg
    20461 atatggtcat caatatcggt gctttgaaat caaaggatga tcaacaagtc caaaaagata
    20521 tcgaagcagt tgttgaagca gcaaaagatc gtgcgcttgt caaagttatt attgaaacat
    20581 cgttattaga tagagaagaa atcatccgag cttgcgaaat agcaaaagct gcaggcgctg
    20641 attttgtcaa aacatcaact ggtttctcaa caggcggagc aaaggtagag gatgtccgtt
    20701 tgatgcgtga aactgtagga ccagaaatgg gtgtcaaagc ttccggcggt atccataacg
    20761 aagaagaagc aatggcaatg atcgaagctg gtgctacaag aatcggaaca agcgcaggag
    20821 tagcaatcgt atctggttca actggtgaag gctattaatt ttttcaaagg agaagctcat
    20881 ggcagaactc aaacaagaat ggatcaacac agctattgaa gcgttagata aagcctatgt
    20941 tccttattcc cattttccgg taggcgcttg tctcgtaacg gaatcaggca agatttatca
    21001 aggcatcaac attgagaatg catcatttgg attaacgaat tgtgccgagc gcacagcttt
    21061 tttcaaagca gtatctgaag gagaaaaaac atttacgcat ctagtcgtag cggggcacac
    21121 ccccgatcct atctctcctt gtggtgcatg tcgacaagta atggcagaat tctgttcccc
    21181 tgatatgccg gttacgctag taggagacaa tggtgtgacg aaggcaacga ccgttcgtgc
    21241 actgctgcct tatgcattta ctgaaaaaga tctataactc atgttcattc agtcagctga
    21301 ctgttgaaaa ataaaacaat tattttagga ggctttacag aaatgaaaaa agcaaaatta
    21361 tttggtttag gtgctgtcgc actagcagca gggttatttt taggtgcatg cggcaacaac
    21421 ggctctactg attcatccgg tggcaaaagc tcatcagaca caacaactgc agcattgatc
    21481 acagacacag gtggtgtaga cgaccgttca ttcaaccagt cagcttggga aggactagaa
    21541 aaatggggga aagatcaagg actttcacga ggaaatgatg gcttccaata tttccaatct
    21601 tcaaatgaat cagattacat cccaaatatt gaccaagcat tgaatgctgg attcaaaacg
    21661 atttttggta ttggttataa actaaaacca gcgatccaag aacaagcaac aaacaataca
    21721 ggtacgaact ttgttattat cgatgatgtc atcgatggat tagataacgt tgtatctgca
    21781 actttcaaag acaacgaagc ttcttatctt gcaggtgttg cagcagctta tacgactgaa
    21841 acaaacgtag tcggctttat cggcggtgta aaaggtgaag taatcgatcg ttttgatgct
    21901 ggattcaaag ccggtgttga tgcaggtgcg aaagaattag gaaaagaaat caaagtattg
    21961 aatcaatatg ctggtgactt ctctgctcca gataaaggac gttcaattgc tcaaggtatg
    22021 tatgcacaaa acgcagatat catcttccat gcttcaggcg gtacaggtaa cggtgtattc
    22081 caagaagcaa aatcattgaa cgaatcaggc gacaaaaaag tttgggttat cggtgttgac
    22141 cgcgaccaat cagacgaagg cgaatacaca ttaaacggcg aaaagaaaaa cttcacattg
    22201 acatctacac tgaaagcagt tggaacagta gtagaagatt tggcacaaaa atcagcggac
    22261 ggtaaattcc ctggcggcga acacactgtt tatggattga aagaagacgg tgttggtcta
    22321 acagaaggac aactttctga cgaagcgaaa aaagcagttg atgaagcaaa agaaaaaatc
    22381 atttcaggtg acgtaaaagt tccagaaact cctgaagaaa actaaaacca tttctatagc
    22441 cttgttagac acatgtttga caaggctatg gtttgattag aacaagaatt gttcatttat
    22501 ccaagctgcc ttgtgacagt ttggataagg gaacaactcc ctgaagtaat gtttgtttca
    22561 aaagaagaac gctcactaag cgtgaatctt agtcaatcgt tgccctagat agaaatcgtt
    22621 aggatgtgaa aaaaaggtgg ttcaagaaaa tattgtcata gaaatgcaga atattaccaa
    22681 agaatttgga acattcaagg caaatgacaa catcaattta caagtaaagg caggagaaat
    22741 tcatgcgttg cttggagaaa acggtgctgg caaatctaca ttgatgaacg tgctttccgg
    22801 attattagag ccgacatcag ggaaaatttt gatgcgtggg aaagaagtaa agatcacaag
    22861 tccgaccaaa gccaaccaat tagggattgg gatggtccat cagcacttta tgcttgttga
    22921 tgcctttact gtaacagaaa acatcgtgtt gggaagcgag cctagtcgcg cagggatgct
    22981 tgaccataaa aaagcacgaa aagagatcca aaaagtttct gaacagtatg gattgtcagt
    23041 caacccagat gcttacgttc gtgatatttc agttggaatg gaacaacggg tagaaatttt
    23101 aaaaacactt taccgaggag cagatgtact gatttttgat gagccgacag ctgtattgac
    23161 ccctcaagaa attgatgaat taatcgtgat catgaaggaa ttagtcaaag aaggcaagtc
    23221 gatcattttg attacgcata agttagatga aatcaaagca gtagctgacc gctgtacagt
    23281 tatccgccgt ggaaaaggaa tcggtacagt caacgttaaa gacgtaacct cacaacaatt
    23341 agccgatatg atggttggaa gaacggtttc attcaaaacg atgaaaaaag aagcgaagcc
    23401 tcaagaagtc gttttgtcta ttgaaaatct agtggtgaaa gaaaatcgtg gattagaagc
    23461 cgtgaaaaac ttgaacttag aggttcgtgc tggcgaagta cttggtatcg ctggaatcga
    23521 tggaaacggg cagtcggagt tgatccaagc tttgactggt ttgcgaaagg cagaaagcgg
    23581 acatatcaag ctaaaagggg aagacatcac taataaaaaa ccccgaaaaa tcactgaaca
    23641 tggtgtagga cacgtgccag aagaccgtca taaatacggg ttagtcctag atatgacatt
    23701 atctgaaaat atcgcactac aaacctatca tcaaaaacct tacagtaaaa acggcatgct
    23761 gaattattca gtaatgaatg aacatgccag agaattgatc gaagaatatg atgttcgaac
    23821 aacgaatgaa cttgttcccg caaaagcttt atcaggcgga aatcagcaaa aagcaattat
    23881 cgctcgtgaa gtcgaccgag atcctgatct gttgattgtt gcaaatccaa ctcgtggact
    23941 ggatgtaggt gcgatcgaat ttattcataa acgtctgatc gaacaaaggg acaaatacaa
    24001 agcagtgtta ctgatcagtt tcgaattaga agaaatttta aatgtttcgg atcgtattgc
    24061 tgttatccat gaaggagaaa ttgtcggtat cgttgatccg aaagaaacat ctgaaaatga
    24121 attaggtcta ttgatggcgg gctatacctt agaagaagca cgaaaagagt tagaaaaggg
    24181 ggctggcgca catgaatgat cgcagcgaaa aattccgtaa tttattagtg cctgtacttt
    24241 cagttgtttt aggattgatc ttaggcgcga tattgatggc tgcttttggt tttcagccca
    24301 tccaaggtta ttcttctatg ctagatgcag cccttggttc gcaaagaggg ataggtgaaa
    24361 cgttaaggca agcaacgccg ttgattttta cagcattagg tttttctgta gctaactcag
    24421 ctgggttttt caatattggg ctttctggac aagcactttg cgggtggatc gtcagtgtct
    24481 gggtggcact tgcgtttcca gatctgccaa aagttgtttt gcttccgtta tgcgtgatct
    24541 taggtgcctt agctggtgcg atggcagcgg ctgttccagg tttacttcga gcattttttg
    24601 gaacaagtga agtgatcgta acaatcatga tgaactatat cttattatac gtaagcacat
    24661 ttgctttaca tgaagtgatg cctgaaagct atcgctcaag tttggactct tccaatatga
    24721 tcagtcaaaa tgcctcactg cggttaccat ggttaacaca agcttttggc ggctctcgcg
    24781 taaactcagg attattttta gcaatcatcg cgcttatttt cgtttggctg ctgatgaaga
    24841 aaacgacttt aggatttgaa atccgttctg ttggattgaa tccttttgct tctgagtatg
    24901 caggaatgag tagtaaacgt acgatcgttt tgtcgatggt catttctggc gctttggcag
    24961 ggcttggcgg tgtggttgaa ggattaggaa cgtatcaaaa cttctttgtt caaacgactt
    25021 cgctatctat tggatttgac ggaatggctg tttccttgct tggttctgga tcagcaatcg
    25081 gtattctttt atcagcgatc ttgttcagta ttttgaagat cggtggactt ggcatgcaga
    25141 caggagcagg tgttccgttt gagatcgtca atgtatcgat tgctttgatt atcttctttg
    25201 tcgggatcaa cttcctgatt cgtttcgtca tggctaaagt cttccaaggg aaaaaacaag
    25261 aagagatcgt tgcgacaata gaatcgaaac caacgaatca aaaccatgaa gggggcgaac
    25321 tataatgtcg actatcgaat taattgcctt gatccttact tctacactgg tttattcaac
    25381 accgctaatt ctaacgtcac taggcggaac tttttctgaa cgaagcggga tcgtaaacgt
    25441 agggctagag ggaatcatgg tcatgggtgc ttttagttcc atcgttttca acttgacgat
    25501 gagtccatca cttggttcta tgacaccatg gatcgctatt ttagtgggtg gcgtcgtagg
    25561 tatcctattt tctctgattc acgcagtagc aacagtcagt ttgcgtgctg accatattat
    25621 cagcgggaca gtaattaatc tgatggcacc tgctttaggt gttttcttga tcaaagcatg
    25681 gtatggtaaa ggtcaaactg ataacatcac gcagaatctg gggtattttt ctttcccagg
    25741 attagcagat attccggtca ttggaccaat tttctttaaa aatacattct tacctgctta
    25801 ttgtgctatt ttgattgctg tcctagcttg ggtgatcatc ttcaagacga aattcggctt
    25861 gcgcctccgt tcagttggtg aaaatcctca agctgccgac acattaggta tcaatgtata
    25921 tgctatgcgt tattcaggcg tactgatttc tggcttgtta ggcggtatgg gcggtgctgt
    25981 ttttgctcag tcgatttccg gtaacttttc agctggtacg atcgttggac aagggttcat
    26041 ctccatggct gctatgatct ttggtaagtg gaatccttta ggggcaatgg gtgcctcatt
    26101 gttctttggt tttgctcaaa gcttgagtat cattggcgca caattaccag ttatttcttc
    26161 gattccgcca gtattgctgc aaattgctcc atatttgctg acaatcattg tgctggttgt
    26221 tttcttaggg aaagcttctg gtccaaaagc aaacgggaag aactatatca aatcaaaata
    26281 atcttttagc gaataaaaag ggtgggttac gggaagtgtt tttcgtctcg ccctttttgt
    26341 tttaaaggca ataaaaataa aaagaaagaa ggaataatca tgtttaaacg cgtacatctg
    26401 atcgtcatgg attcagtagg aattggagaa gcaccagatg ccgataagtt cggtgatgtt
    26461 ggttctgata cattaggaca cattgcgaaa gaagcaggat tgaacgttcc acatttagag
    26521 cagcttggtt taggaacgat ccgtcctctt gaaggtgtgg aatcagtgaa agatcatcaa
    26581 ggctatgcga cgaaattaga agaagtttct gtagggaaag atacgatgac aggccactgg
    26641 gaaatcatgg gattgaatat caaaactcca tttcgtgtgt ttcctaatgg attccctgat
    26701 gatctgatca aacaaatcga agaattttct ggccgtaaag tcgtatgtaa caaaccttat
    26761 agcggaacag ctgttattga cgattacggt gaacaccaaa tgaaaacagg cgatttaatt
    26821 gtatatactt ctgccgatcc tgtccttcag atcgcggcac atgaagaaat cattccttta
    26881 gaagaattat atcggatttg ccaattcgtt cgtgatataa caaaagatga tccgtatatg
    26941 attggccgaa tcattgcacg tccgtacgtg ggtgaaccag gagacttcac acgaacaagc
    27001 aatcgccatg attatgcgtt gaatccattc ggacacacag tccttgattc tttgaaagaa
    27061 gcaggaaagg acgtgattgc tgttggaaag atcaacgata ttttcaacgg acaaggaatc
    27121 actgaatctg tccgcacgaa aagcaatatg gatggcgttg accagctgct gaaagttatg
    27181 aaaaacgatt tttcaggaat cagctttacc aatttagtag attttgatgc attatacggt
    27241 catcgtcggg atgtagtcgg ctatgcgcat gcgattgaag aatttgactt acgaattcca
    27301 gaattgcttg aagcgatgga agaagacgac ttgctaatga ttacagccga tcacggaaac
    27361 gatccgacgt ttactggtac cgatcatacg cgagaatatg taccgttatt ggtttacagt
    27421 aaaaaaatga aagaacaagg ggcattgcct caaggatatt attcagatat ttcagcaacg
    27481 attgctgaga actttagtgt ttcttcgact gaaaatggcg aaagcttctt gaaattatta
    27541 aaataatctt tttaaatagg agaaagcaaa tgaccaagtt aagcgagatg ttacaagaaa
    27601 cgacccagtt tatccttgat aaaggagtaa aagaaattga ttttggtttg attctaggtt
    27661 caggattagg tgaattagca gatgaaatcg aagatgctgt aatgatttct tatgatgaga
    27721 tcccgttctt tcctgtttcg acagtagttg ggcatgcagg aaagctagtt tatgggtctc
    27781 tggcaggcaa gaaagtgtta gccatgcaag ggagattcca ctactatgaa ggtcattcca
    27841 tgcaaacagt gacctatcca gtacgtgtga tggccggact aggggcccat tcagtcattg
    27901 tgactaatgc atgtggtggt gtcaacgaat catttgtacc aggagatttg atgctgatta
    27961 ccgatcatat caattttact ggtgaaaatc ctttgatcgg tccgaatgaa gatgcaatgg
    28021 gccctagatt tccagatatg agtcaagctt atacgcctga ctatcaagca acagcaaaag
    28081 cagcagcaga aaaactaggt ttggatctta aagaaggcgt atatatgggc tattctggac
    28141 caacttatga aacgcctgcg gagatccgaa tgtctcggac aataggagcc gatgcagtag
    28201 ggatgtcgac tgttccagaa gtcatcgtag cagctcacag cggattaaaa gtgttaggaa
    28261 tctcttgtat taccaactta gcagcaggta tgcaagcgaa tctgagccat gaagaagttg
    28321 tagaaacaac tcagagagtc aaacaatctt ttaaagcatt ggttaaagaa acattgacat
    28381 tattgtaata ggaggaacaa taaaatgagt gtgcatattg aagcgaaaga aggcgagatc
    28441 gccgataaga ttttacttcc gggagatcct ttgcgagcta aatatattgc ggagactttt
    28501 ttggaagatc cagtttgtta caatcaggta cgcggtatgt taggctttac cggcaaatat
    28561 aaaggagaac gtatttctgt tcaaggaaca ggaatgggaa tgccatcagc caccatctat
    28621 gcccatgaat tgatccagtc gtatggtgta aagaaattga ttcgagtagg tacatgcggt
    28681 gcattgtcaa aagacgtcca tgttcgtgat ttagtactag cacaaggtgc agcgaccagt
    28741 tcttcgatga tcgaaaaaaa tttccaagcc tttcattttc ctccaatcag tgatttcaat
    28801 ctattactga aagcatatga gatagctaaa gaaaagggct atacggttca tgtaggaaat
    28861 gttctttcgg aagattcttt ctacaaagat gatttgacag agacgttcca attagctgaa
    28921 cttggcgttt taggagtgga aatggaagca gcggctcttt attacttagg cgcgaaatac
    28981 catgtgcaga cattaagttt gatgaccgtt agcgaccatt tgatcaccgg tgaagaaaca
    29041 acagctgcag aacgtcaatc tacgttcaat gaaatgatcg aagtaggatt ggaaacagcg
    29101 atagcagaat aagaaggaaa tgaaaaagct gccctgcatt aagtaataag aacatccaag
    29161 caaaaatagc tcctcatgtt tttgacttgg ctggacttat tattgcaaat gtaagctttt
    29221 gaacaccgtt taccaagagg ttgtgaatct gccgtttaac tctgagtcac aaggaacaat
    29281 ttcaaaaaac agcttttcat gtttgatgaa tattgtgact tgtgaccgag aagttggcag
    29341 gagaaaaccg tttatacgaa aaaagggact gtgacaaatc gtgtcacagt ccctttttta
    29401 tagtgttttt tataggtagt attttttagc aactgttaag tcatcattta attcataaac
    29461 aagtggttga cctgttggaa tttcaagatc catgatgtct tcgtcagaga taccttcgat
    29521 atgtttagct agggcgcgta atgagttacc atgtgcagct actaaaacag ttttgttgtc
    29581 tagtaatgct ggagcaattt catcttgcca gaatggcaaa gcgcgttcta atgtcacttt
    29641 caagttttct ccaccaggaa tatcgcgttt gtctaacatt gcgtaacgac ggtcatttgc
    29701 tgcagaacct tcgtcagttt cttccattaa tggaggaaga gtatcgtaag aacgacgcca
    29761 gatgtgcact tgttcgtcgc cgtatttttc agctgtttct tttttgttca aaccttgtaa
    29821 tttaccatag tgacgttcgt ttaagcgcca tgatttgatt tgtggtaccc aaagttgatc
    29881 tgaatattct aagatcaagt tacatgtttt gattgcacga gttaaaacag aagtgtaagc
    29941 aacatcaaat tcgattccag cttctttgat tttgcgtccg ccttcttttg cttcttcgat
    30001 tccttctggt gctagattta catcagccca accagtaaat tggttcaatg cattccattc
    30061 actaagaccg tgacgagaaa aaactaattt tggcatgttt agctctcctt ttaagatagg
    30121 tttatatttc tgaaattttc agttctgttt tattgtacaa ttttttggaa gagatttcca
    30181 tcgaaaaatt agataagatt ctatgagaaa acaagtagag aatataaaaa aacgaacagt
    30241 gcccacgcaa ctgttcgttt ttaggtatta gatgcataat tgctcgattt ctttttcagg
    30301 taaattgata tatttataac atgttccaac tgaaacaccg cattttgaag aaataaattg
    30361 gatcgtttct tttttttcat aatagagacg tcggattttt ttgatcgttt ttgcatcgat
    30421 ttttggtctt ccgccgattt ttcctttttt acgagcttca tctaatccaa ctaatgtacg
    30481 ttctttgatc aatgcgcact ccatgtttgc taggttttcc attaattgga aatagatttt
    30541 tcccatttcg cgcgtatcga ttccttcatc aagactaatc aagtgcagcc cgcgatcttt
    30601 gaataattgc gtgagttctg ttaattgccg cgtagatttg cctaatcgat gaagttggca
    30661 aatgactagt gcatcgcctt gttgcatgtc agcgatcact gactccaaat gaacagcagt
    30721 agaatcattt tgttccactt catatgtctc atggaaaatt tcttgacaac caaagtcaga
    30781 taattgatca acctgagttg ttaaatcaac gtcatttatg gttgtgcgtg cataaccaat
    30841 aattttcatg gtgattcctt tctagcagtg attttctaac tggatataac agtaaaaatg
    30901 tagcataaag tacgtcaaaa agatagtttt ttcgcaaatt ttccgctgtc tttttttatt
    30961 ggatgatcaa gcagtttttc tttatttacg acggattgcg tcaagaatct caacgccttt
    31021 ttctgagccg acaactactt tagaaaccat gtttaggaaa agcccatgtt ctactacccc
    31081 aacaagttca tctaaatagg tagctaagct ttctggctgt tcgatgactt ctagatgcag
    31141 atcaatgatg taatgaccac catctgtggt taagacttct ccgttttcgt ctgtacgcaa
    31201 gacgggttgg aatctttttt cttcaaaaat atgaagtagc tgttgggagc cataagggat
    31261 cacttcaact gggagtggga acttcccaag tttatcgact aatttagaag aatcaacgat
    31321 ccaaatcgtt tcttttgagt acgtagcgac tattttctcg aaaagcaaag cagcgccgcc
    31381 gcctttgatc ccttgaaaat cagcactgat ttcatcagca ccgtctattg tcaaatcgac
    31441 tacggggact tcatcaatac tctttaacgg tattcctaat ttttccgctt gttcttttgt
    31501 cgcatttgaa gtagtaactc ctgtgatacg taaaccttct tcacgcatac ggcgaccaat
    31561 ctcttctacc atataatagg cggttgaacc ggtcccgaga cctacgatca tcccgtcctt
    31621 tacgaatttc gcggcttcaa tgccagctaa ttgttttaaa ttcatctttg cgtccacctt
    31681 tttttagttt gagcatcgtg ctttcctcac tattttagcg tgatttacgg taaaaagata
    31741 ccattctttt ttattccaat aaagcttttt caaaaaaaat tgcatctctt gttgacatgt
    31801 tttttcattc gtgatattct aacaaaggtg ctttatctac aggtttctgt gaagaaacgt
    31861 gctgactgtt tatcaaagca tatttgataa ggatgcagac attgcattta ttttttatca
    31921 caaaaaaaga accacctgga tgtgtggaac tagacgcaaa tcgtgggaag gaggaaacaa
    31981 ggaatgccta caattaacca attagtacgt aaacctcgta aatcaaaggt ggaaaaatct
    32041 aattcacctg ctttgaacaa aggatataac agctttaaga aaactcaaac taacgtaaac
    32101 tcacctcaaa aacgtggggt agcaactcgt gtgggtacaa tgacacctaa aaaaccgaac
    32161 tcagctttac gtaaatatgc acgtgttcgt ttgtcaaact taattgaagt tactgcttat
    32221 atcccaggga ttggtcataa cttacaagaa cacagtgttg tattattacg cggtggtcgt
    32281 gtaaaagact taccaggggt acgttaccat atcgttcgcg gtgctttaga tactgctggt
    32341 gttaacgacc gtaaacaaag ccgttctaaa tacggaacta aaaaacctaa agcttaattt
    32401 taattattaa acaaatgaaa cttgcttcta ctatatagaa gtaattacta tgaacaatat
    32461 ttcggaagga ggagttacgg atgccacgta aaggtcctgt tgcaaaacgt gatgttttac
    32521 cagatcctat ttataactca aaattagtaa ctcgcttgat caaccgtgta atggttgacg
    32581 gaaaacgcgg gattgcagct aacattatct ataatgcctt tgacttgatc aaagaatcta
    32641 caggtaacga tccattagaa gtgtttgaac aagcaatgaa aaacgttcaa cctgttcttg
    32701 aagttaaagc acgtcgtgtt gggggttcaa actaccaagt accagttgaa gttcgccctg
    32761 aacgccgcgt tactctaggt cttcgttggg tagtaaacta cgctcgcttg cgcggtgaac
    32821 acacaatgga agaacgctta gcaaaagaaa tcatggatgc tgcaaacaac acaggtgctt
    32881 ctgttaaaaa acgtgaagac actcataaaa tggctgatgc caaccgtgcg tttgctcact
    32941 atcgctggta agattctctc tgtttggtcg gcttttataa gtcgacctca gatagtgtcc
    33001 ttatttttag caactagata gtaaagagag gagaaacaag gagaatgacc agagaatttt
    33061 ctttacaaaa cactcgtaat atcggtatca tggcccacat cgatgctggt aaaacgactg
    33121 ctactgaacg tatcttgtac tatactggta aaatccataa aattggtgaa actcatgaag
    33181 gagcttcaca aatggactgg atggaacaag aacaagaacg tggtattacg atcacttctg
    33241 ctgctactac agcacaatgg aagggtcacc gaatcaacat catcgacaca ccaggacacg
    33301 tagacttcac agttgaagtt gaacgttcat tgcgtgtact tgatggtgct gtaactgttc
    33361 ttgatgcaca atcaggtgtt gaacctcaaa ctgaaacagt ttggcgtcaa gcaacaactt
    33421 atggtgtacc tcgtatcgta tttgcaaaca aaatggacaa gatcggtgct gacttcttat
    33481 attcagtatc aactttacat gaccgtttgc aagcaaacgc tcacccaatc caattaccaa
    33541 ttggtgcgga agacgacttc acaggaatta tcgaccttgt aacaatgaag gctgaaatgt
    33601 atacaaacga tcttggaaca gagatcgaag aaacagaaat tcctgaagaa taccgcgaat
    33661 tagctgaaga atggcgcgaa aaattggttg aagctgtagc tgaaactgat gaagaactta
    33721 ctttgaaata cttagaaggt gaagaaatca ctgaagctga gttaaaagaa ggtatccgcc
    33781 gcgcaacagt taacgtagaa ttctacccag tactatgtgg ttcagcattt aagaacaaag
    33841 gggttcaatt actattggac gctgttcttg attacttgcc atcaccgtta gatatcccag
    33901 ctatcaaagg gattgaccct aaaactgatg aagaagtaga acgtcctgca gacgacagcg
    33961 ctcctttctc agctttagca tttaaagtta tgactgaccc attcgtaggt cgtttaactt
    34021 tcttccgtgt gtactctggt gttttacaat caggttcata cgtacaaaac gcaacaaaag
    34081 gcaaacgtga acgtgttggt cgtatcctac agatgcatgc caactctcgt tcagaaatct
    34141 ctgaagttta cgctggagat atcgctgctg ctgttggttt gaaagacaca actactgggg
    34201 acacactatg tgatgaaaag agtctagtta ttcttgaatc catggaattc ccagaaccag
    34261 ttatccaagt cgctattgaa cctaaatcaa aagctgacca agataaaatg ggtgtggctt
    34321 tacaaaaact atctgaagaa gatcctactt tccgtgctga aacaaacgtg gaaacaggcg
    34381 aaacaatcat cgctggtatg ggtgaacttc acttagatat catcgttgac cgtatgcgtc
    34441 gtgaattcaa agttgaagct aacgtaggtg cgcctcaagt atcttaccgt gaaactttcc
    34501 gtgctggcac tcaagccgaa ggtaagtttg tacgtcagtc tggtggtaaa ggtcaatacg
    34561 gtcacgtatg gattgaattt acaccaaatg aagaaggtgc tggtttcgaa tttgaaaatg
    34621 cgatcgtcgg tggggttgtt cctcgtgaat acattccagc tgttgaaact ggtttgaaag
    34681 atgctatgga aaatggtgtc ttagctggct atccattagt agatatcaaa gctaaacttt
    34741 acgatggttc ataccatgat gtcgattcaa atgaaactgc cttccgtgtt gctgcttcta
    34801 tggcattacg tgcagcagct aagaaagcta atccagtaat ccttgaacca atcatggctg
    34861 tagaagttgt tatccctgaa gattatttag gtgatgttat gggtcacgta actgctcgtc
    34921 gtggacgtgt tgaaggtatg gaagctcgcg ctggcggcca acaagttgtt cgtgcgatgg
    34981 ttccacttgc tgaaatgttt ggttatgcta caactcttcg ttctgctact caaggtcgtg
    35041 gtacatttac aatgacattt gaccactacg aagatgtacc aaaatcagta caagaagaaa
    35101 tcattaagaa aaacggcgga aaagctgaat aatttcttaa aaccggatag tcctgcaaag
    35161 agattatcca aagagactat atttttaata tagtttcgtg taaaataatt atcgatgata
    35221 tgggcatgtg gtgtaccttg tgtacatcat atacctatca gacaaataaa aaatatattg
    35281 tatttttagg aggacatttt aaaatggcaa aagaaaaatt tgaccgttct aaaccacacg
    35341 ttaacattgg tactatcgga cacgttgacc atggtaaaac tacattaaca gctgcaatca
    35401 caactgtact atctaagaaa aacggcggcc aagctatggc ttacgatcaa atcgatggtg
    35461 ctccagaaga acgcgaacgc ggaatcacaa tctcaacagc tcacgttgag tatgaaacag
    35521 atacacgcca ctacgctcac gttgactgcc caggacacgc ggattacgtt aaaaacatga
    35581 tcactggtgc tgctcaaatg gacggagcta tcttggtagt ttctgctgct gacggcccaa
    35641 tgcctcaaac tcgtgaacac atcctattgt ctcgtcaagt tggtgttcct tacatcgttg
    35701 tattcttgaa caaagtagac atggttgatg acgaagaatt actagaatta gttgaaatgg
    35761 aagttcgtga cctattaaca gaatacgaat tccctggtga cgatgttcct gtagttgctg
    35821 gatcagcttt gaaagctcta gaaggcgacg cttcatacga agaaaaaatt cttgaattaa
    35881 tggctgcagt tgacgaatac atcccaactc cagaacgtga caacgacaaa ccattcatga
    35941 tgccagttga agacgtgttc tcaattactg gacgtggtac tgttgctaca ggtcgtgttg
    36001 aacgtggaca agttcgcgtt ggtgacgaag ttgaagttgt tggtattgct gaagaaactt
    36061 caaaaacaac agttactggt gttgaaatgt tccgtaaatt gttagactac gctgaagctg
    36121 gagacaacat tggtgcttta ctacgtggtg ttgcacgtga agacatccaa cgtggacaag
    36181 ttttagctaa accaggtaca atcacacctc atacaaaatt ctctgcagaa gtatacgtgt
    36241 tgacaaaaga agaaggtgga cgtcatactc cattcttcac taactaccgt ccacaattct
    36301 acttccgtac aactgacgta acaggtgttg ttgaattacc agaaggaact gaaatggtta
    36361 tgcctggcga caacgtaaca atggaagttg aattaatcca cccaatcgct atcgaaaacg
    36421 gtactaaatt ctcaatccgt gaaggcggac gtacagttgg tgccggtgtc gttacagaaa
    36481 tcaaagctta attcacttaa gttttaaagt aaagcaacat ttattcggac gtaatcgctc
    36541 ggaacgaagg tcttaggatc tttgttccga gcttttttat ttattgcagg atcaggaaaa
    36601 aagacaggag tattataaat agaagaaaca cgaaataaaa gagtcagtat tttctaaatg
    36661 aaactttatg aaaacaaaat gttttttaca agaaaaagcc ttgctttgtg agtttatata
    36721 tattataatg acaaaggtgc gtttacttta gtgaattgca cagacgtaat cagaatgatt
    36781 tcgtcggcta agaaacgaga ggttgcgaca cgcccggacg ctttgccacg aacgagcgtg
    36841 acggaaattt tcgtggagct atgtctactt ttcaaaatag gcgaaggagg gaacaagatg
    36901 gcaaaacaaa aaattcgtat ccgtttaaaa gcgtatgaac accgtatttt agatcaatca
    36961 gcggataaaa tcgtggaaac tgcaaaaaga actggagcta gcgtgtctgg tccaattcca
    37021 ttaccaactg aacgcagtct ttacacaatc atccgtgcga ctcataaata caaagattca
    37081 cgcgaacaat tcgaaatgcg tacacacaaa cgtcttatcg acattgtgaa cccaacacca
    37141 aaaactgttg atgctttgat gaagctagac ttaccatctg gtgtaaacat cgaaatcaaa
    37201 ttataaaact aaaacaaaaa taatggaggt gtactcatga ccaaaggaat cttagggaaa
    37261 aaagtgggaa tgacacaaat cttcactgaa tctggtgaat taattccagt aactgttgta
    37321 gaagctactc caaacgtagt actacaagtg aaaacaatgg aaacggatgg ctacgaagct
    37381 attcaagttg gttaccaaga tatgcgtgaa gttttatcaa acaaacctgc gaaaggtcat
    37441 gttgcaaaag caaacacggc tcctaagcgc ttcattcgtg aattcaaaaa tgttgagcta
    37501 ggagaatatg aagtaggaaa agaaattaca gtagatgttt tccaagcagg agacattatt
    37561 gatgtaacag gtactacgaa aggtaaagga ttccaagggg ttatcaaacg ccacggacaa
    37621 agccgcggac caatggctca cggttctcgt taccatcgtc gtcctgggtc aatgggtcca
    37681 gttgcaccta accgtgtatt taaaaacaaa cgtcttgctg gccgtatggg tggcaaccgc
    37741 gtaactatcc aaaacttgga agttgtacgt gtcgatgcag aaagaaatgt tatcttgatc
    37801 aaaggtaatg ttcctggagc taaaaaatct ttaattacta tcaaatcagc tgtgaaagct
    37861 aaataattgt aggaagaaag gaggaactaa ggaatgccga atgtagcatt attcaaacaa
    37921 gatggaagcc aaaacggtga aatcacactt aacgaagaaa tcttcggaat tgaacctaat
    37981 gaaagtgttg tttacgatgc aatcgtaatg caacgtgcgt cattaagaca aggaacacac
    38041 gcggttaaaa accgtagcgc tgttcgcggc ggcggacgta aaccatggcg tcaaaaagga
    38101 acaggtcgtg cacgtcaagg atctatccgc tcaccacaat ggcgtggagg tggcgtagtc
    38161 tttggaccaa caccacgttc ttacagctac aaacttccta aaaaagttcg tcgtttagca
    38221 atgaaatctg tattgtcaga aaaagttgct gaaaacaact tggtagcaat cgaaggcttg
    38281 aacttcgacg caccaaaaac aaaagaattc aaacaagttc ttgctaactt atctatcgac
    38341 tctaaagtat tggttgttct agaaaaaggt aacgactttg ctgctttatc agcacgtaac
    38401 ttaccaaacg tttctgtagt aacttctgat aatgtgagtg tgctagacgt tgtatcaaat
    38461 acaaaagtgt tagcaacaca aactgctctt actcaaattg aggaggtgct tgcataatga
    38521 acttactaga cgtaatcaaa cgcccagtga tcactgaaaa atcaatgctt gccatggatg
    38581 acaagaaata cacttttgaa gtggacactc gcgcaaacaa aactttagta aaacaagcgg
    38641 tagaagctgc ttttgacgtt aaagtcaaaa acgtgaacat cgtgaacgta cgtgcgaaat
    38701 tcaaacgtat gggcaaatat gcaggatata caaaaaaacg tcgcaaagcg atcgttacat
    38761 tgactgaaga ttcaaaagaa atccaattat tcgaagctgc tgaataagca ctaatagata
    38821 actcattaaa ttaggaggga aaaacgtggc gattaaaaag tacaaaccta cctcaaatgg
    38881 tcgtcgtaac atgacaggtt ctgattttgc tgaaatcaca acatcaacac ctgaaaaatc
    38941 attgttacaa ccattgaaaa accatgctgg acgtaacaac aacggccgca tcactgtacg
    39001 tcatcaaggt ggcggacaca aacgccaata tcgcgtgatc gacttcaaac gtaacaaaga
    39061 taacgttgtt gcaactgttc aaacgatcga atatgatcca aaccgttcag ctaacatcgc
    39121 gttagtacac tatgaagatg gagtgaaagc ttacatctta gcacctaaag gactagaagt
    39181 gggtatgaaa cttgtttcag gtccagaagc agatatcaaa gtcggaaatg ctcttccatt
    39241 agaaaacatt ccagttggta ctgttatcca caacattgaa atgaaacctg gtaaaggtgg
    39301 acaattgatc cgttctgctg gtacaagtgc tcaagtactt ggtaaagaag gcaaatacgt
    39361 attgatccgc ttgaactctg gtgaagttcg tatgatctta gctgcatgcc gtgctacaat
    39421 cggttctgta ggtaacgaac aacacgaatt aatcaacatc ggtaaagctg gacgctctcg
    39481 ttggatgcgt aaacgcccaa ctgtacgtgg tagcgtaatg aaccctaatg atcacccaca
    39541 cggtggtggt gaaggtaaag ctcctatcgg acgtaaagca ccagtttctc cttggggtca
    39601 accagctctt ggttacaaaa ctcgtaacaa aaaagctaaa tcagacaaac ttattgttcg
    39661 tcgtcgcaaa aataaataat tactttgacc atgtgtcgca cattttgaga ggaggttcac
    39721 catgggtcgt agtttaaaga aaggaccttt tgtcgatgag catttaatga aaaaagttga
    39781 agctcaagca ggggttgaaa agaaaaaagt tatcaaaact tggtcacgcc gttcaacagt
    39841 tttcccaagt tttgttggat ataccatcgc agtttatgat ggacgtaaac atgtaccagt
    39901 ttacatccaa gaagacatgg taggacacaa attaggtgaa tttgcaccta caagaactta
    39961 tcgtggacat gctgctgacg ataagaaaac gaaacgctaa tttgagggga ggataaatcc
    40021 aaatggcaga acaaatcaca tcagctaaag caactgcaaa aacagttcgc acttcacctc
    40081 gaaaagcccg tttagtaatt gaccttatca gaggtaaaag tgtcgcagat gcaatttcaa
    40141 tcttgaaatt cacaccaaac aaatctgctg gaatcattga aaaagtattg atgtcagcag
    40201 ttgcgaatgc agaaaataac tttgacttag atgttgaaaa cttggtagta agcgaagctt
    40261 ttgttaacga aggacctaca atgaaacgtt tccgtcctcg tgcaaaagga tcagcttctc
    40321 caatcaacaa acgtacaagt catatcacgg tagtcgtatc agaaaaataa ggagggataa
    40381 tcagtgggtc aaaaagtaca tccaattgga atgcgtgtag gcatcatccg cgactgggat
    40441 gctaaatggt atgctgaaaa agagtatgct gagttcttac acgaagattt aagaatccgt
    40501 aaatttattt caactaaact tgctgatgct gctgtatcaa cagttgaaat cgaacgcgca
    40561 acaggtcgcg taaacatttc aattcacaca gctaaaccag gtatggttat tggtaaaggc
    40621 ggttctgaag tcgaaagcct aagaaaagaa ttaaacaaat taactggtaa aagaatccat
    40681 atcaatatcg tggaaatcaa aaaaccagat ctagatgcaa aattagtagg tgaaggaatc
    40741 gcacgtcaat tagaaaaccg tgttgctttc cgtcgcgctc aaaaacaagc catccaacgc
    40801 tcaatgcgtg ctggcgctca agggatcaaa actcaagtat ctggtcgtct aaacggagca
    40861 gatatcgctc gttcagaagg atactcagaa ggaactgttc ctcttcatac tttacgtgcg
    40921 gacattgatt acgcatggga agaagcagat acaacatacg gaaaactagg agttaaagtg
    40981 tggatctatc gtggagaaat tcttccaact aaaaaaaaca ctgagaaagg agggaaataa
    41041 tcatgttagt acctaaacgt gtaaaacacc gtcgtgaatt ccgcggaaaa atgcgcgggg
    41101 aagctaaagg cggaaaagaa gtagcattcg gtgaatacgg tttgcaagct gttgattcac
    41161 attggatcac aaaccgccaa atcgaagctg ctcgtatcgc aatgactcgt tacatgaaac
    41221 gtggtgggaa agtatggatt aaaattttcc ctcacaaatc ttatactgcc aaagcaattg
    41281 gggtacgtat gggttctggt aaaggggcac ctgaaggatg ggttgcacca gtaaaacgtg
    41341 gtaaaatcat gtttgaaatc gcaggcgttc ctgaagaagt agctcgtgaa gcgttacgtc
    41401 tagcttctca caaattacca atgaaaacta agatcgtaaa acgtgaggaa atgggtggtg
    41461 aatcgaatga aggttaaaga aatcagagaa ttaaccactg ccgaaatgct tgatcaagaa
    41521 aaacaattaa aagaagaatt gtttaatctt agattccaat tagcaacagg tcaattagaa
    41581 aacactgcac gtattaaaga agtacgtaaa tcgattgcac gcatcaaaac tgtgttgcgt
    41641 gaacaagcta agtaattatg gaaggaggcc attaagcgta tgactgaagg aagaaatcaa
    41701 cgcaaagttt atcaaggtcg cgtggtttca gacaaaatgg ataaaacaat taccgttgct
    41761 gtcgaaacaa agaaaaacca ccctatctat ggcaaacgca tgaactattc taagaaatac
    41821 aaagtgcatg atgaaaacaa cacagcaaaa gttggcgaca tcgtgagaat tatggaaact
    41881 cgtccattat cagctacaaa acgtttccgt ttgttggaag tagtcgaaga agcagttatt
    41941 atctaataac acgagatttt cctatataga ttgaatgcaa aatctgaaag gaggatacac
    42001 aacgtgatcc aagcagaaag tcgtttaaaa gtcgctgata actcaggcgc acgtgaaatt
    42061 ttgacgatca aagtccttgg tggatctggt cgtaaaactg ctaacatcgg tgatgtgatc
    42121 gttgctacgg ttaaacaagc aacgccaggt ggggttgtca aaaaaggtga cgtcgttaaa
    42181 gccgttatcg ttcgaacaaa atcaggagct cgtcgtacag acggttcata tattaaattt
    42241 gatgaaaatg ctgcagtgat tattcgtgat gataaaagcc ctcgtggaac acgtatcttt
    42301 gggccagttg cacgtgaact acgtgaaaac aacttcatga agatcgtttc actagcacca
    42361 gaagttttat aatcttgaga cacttatcaa aggaggtgcg aaacagttat gtttgttaaa
    42421 aaaggcgata aagttaaagt tatcactgga aaagacaaaa acaaagaagg cgttgtacta
    42481 gcagcgttcc cgaaaaaaga taaagttatt gtcgaaggtg taaatgtcgt taaaaaacac
    42541 caaaaacctt ctcaagctgc tcctcaaggt ggaattgttg agatggaagc gccaattcat
    42601 gtttctaacg tgatggtggt tgactctact ggtgtagctg gccgagttgg ctacaaagaa
    42661 gtagacggca aaaaagtccg tgtttctaaa aaaaccggtg aagttttaga taaataaaag
    42721 tttaaggaag gagggtctta ctgaatgaac cgcctaaaag aaaaatatct taaagaagta
    42781 actccatcat tgatggaaaa atttaactat agctctgtta tgcaaacacc taaagttgaa
    42841 aaaatcgtca tcaacatggg tgttggtgat gcggtatcaa acgctaaaaa cttagataaa
    42901 gcagttgaag aattaacttt gatcactggt caaaaaccaa tgattacaaa agctaaaaaa
    42961 tcaatcgctg gattccgttt acgtgaagga atgccaattg gtgcaaaagt tacattgcgc
    43021 ggagaaagaa tgtacgaatt cttagacaaa ttggtttctg tttcactacc acgtgtacgt
    43081 gacttccatg gtgtaagtaa aaaagccttt gacggtcgtg gaaactatac tttaggtatt
    43141 aaagaacaat tgatcttccc agaagtagat tatgatttag tagataaagt acgtggtatg
    43201 gacatcgtta ttgtaacaac agcaaactct gatgaagaat ctcgtgagtt gttgacacaa
    43261 ttaggcatgc cattccaaaa ataattaaag gaggcgaact acgtggctaa aaaatcaatg
    43321 attgctaaaa acaaacgtcc tgcaaaattt tcaacacaag cttatactcg ttgcgaacgt
    43381 tgcggacgtc cacattcagt ttatcgtaag ttccatcttt gccgtatttg cttccgcgaa
    43441 cttgcctata aaggtcaaat tcccggcgtg aagaaagcta gctggtaaaa agtaaattcg
    43501 cataagaagg aggtaaaaga tcaatggtca tgacagatcc aattgcagat tttctaactc
    43561 gtatccgtaa cgcaaacatg gttaaacatg agtctttaga agtacctgca tcaaaaatta
    43621 aacgtgacat cgctgaaatc ttgaaacgtg aaggtttcgt tcgtgatgtt gaatacatcg
    43681 aagatgacaa acaaggcgtg atccgtgttt tcctaaaatt tggtaaaaac ggcgaacgtg
    43741 ttatcacaaa cttaaaacgt atctctaaac caggtttgcg tgcttatgtc aaagctgacg
    43801 aagtaccaaa agtattgaac ggtttaggta tcgcaatcat ttcgacttct gaaggtgttg
    43861 tcactgataa agaagctcgt gaaaaaaata tcggcggcga agttatcgct tacgtatggt
    43921 aatcaagtag aatctacaag gaggtgtctt taagtgagcc gtatcggaaa taaagtcgtt
    43981 gttatccctg aaggagtaac agttactcaa gacggaaaca acgttacagt taaaggacca
    44041 aaaggtgaat tgactcgtac tttttcagca gatatcaaaa tgaatatcga aggaaacgaa
    44101 gtaactttca ctcgtccaaa cgacagtaaa gaaatgaaaa caattcatgg aacaactcgt
    44161 gcgaacttca acaacatggt tgtaggtgtc agcgaaggtt tccaaaaagc gttagaacta
    44221 atcggggttg ggtaccgtgc acaattacaa ggatcaaaac ttgtattgaa cgttggttac
    44281 tcacatccag tagaaatcac accaccagct ggagtaactg ttgaagtacc agctaacaca
    44341 caagtaattg ttaaaggtgc gaacaaagaa gaagttggcg aattagctgc aaacattcgt
    44401 ggaactcgtc ctccagaacc ttataaaggt aaaggtattc gttatgttgg cgaatatgta
    44461 cgccgtaaag aaggtaaaac tggtaaataa tcgtttagaa aaattgcata gaagccagca
    44521 gcaggaaaaa ggcatttggc tgaagtttac cttcaaaaaa atacattttt acttgtgccg
    44581 cagcttctat ttttcgaaac gtatttactg cttgaaatag tagtacaata aaaataaaga
    44641 ggtgacaatt gtgattacaa aaccagataa gaacaaaaca cgtcaaaaga gacatcgtcg
    44701 tgtacgtaac aatatctctg gtactgctga gcgcccacgc ttgaacgttt ttcgttctaa
    44761 caaaaacatc tacgctcaaa ttattgatga cgtagcgggt gtaacgctag caagtgcctc
    44821 taccttggat aaagaaattt caggtggaac aaaaacagaa gcagcacaag ctgtcggtaa
    44881 actagttgct gaacgtgcag ctgaaaaagg cattaaagta gtagtctttg accgtggtgg
    44941 atacctttac catggccgtg tgcaagcttt agctgaagct gctcgcgaaa atggactaga
    45001 attttagaag aaggaggaat accattcatg gtttatatcg atccaaaaca tttggaatta
    45061 gaagaccgcg ttgttgcaat taaccgtgta acaaaagttg ttaaaggtgg acgtcgtcta
    45121 cgttttgctg ctttagttgt tgtcggtgac aaaaacggac acgtagggtt tggtacaggt
    45181 aaagctcaag aagttcctga agcaatccgt aaagcaattg aagatgcgaa gaaaaactta
    45241 gttgaagtac ctatggttgg ttcaacaatc ccacacgaag taatcggtgc ttttagtggt
    45301 ggtagaatcc taatgaaacc tgctgtagaa ggttctgggg ttgctgctgg tggaccagtt
    45361 cgtgccgtat tagaattagc tggggtagca gatatcactt ctaaatcttt aggctcaaat
    45421 acaccaatca acgttgttcg cgcaacagtt gaaggtttga aacaattgaa acgtgcagaa
    45481 gaagttgcag aacttcgcgg caaatcagtt gaagaattaa tcggataagg aggacaacaa
    45541 taatggctga attaaaagtt actttaaaac gcagtattat cggacgtcct caaaaccaac
    45601 gcgatacagt taaagcgtta ggtcttacaa agattaacag ttcagtggtt aagccttcta
    45661 atgaagcaat taaaggcatg atcaatactg tttctcacct agtggacgtt gaagaaattt
    45721 aatttagaaa aatcataact agaacattaa ggaggtgcca aacctatgaa acttcatgaa
    45781 ttaaaacctg ctgaaggttc acgccaagta cgtaaccgtg ttggtcgtgg tacttcatct
    45841 ggtaacggaa aaactgccgg acgcggacaa aaaggacaaa aagctcgttc aggcggcggt
    45901 gtgcgtttag gatttgaagg gggacaaaca ccattgttcc gtcgtttacc aaaacgagga
    45961 ttcacaaacg tgaaccgtaa agactatgca gtcattaatc ttgatgtatt gaaccgcttt
    46021 gaagacggag ctgaagtaac tcctgttgct ttagttgaag ctggaatcgt gaaaaacgaa
    46081 aaagctggaa tcaaagtatt agctaatggg gaactaaaca agaaattaac tgtgaaagca
    46141 gcaaaattct ctaaagcagc tcaagaagca attgaagcgg ctggtggatc aatcgaggtg
    46201 atctaatgtt tagactatta aaagatgctt ttaaagtaaa agacattaga tcgaaaatcc
    46261 tatttactgt atttgtgttg tttgtatttc gcctaggtgc acatattact gtaccaggcg
    46321 taaatgctgc ttcattgcaa aacctttctt ctttgccatt tttcaacatg ttgaacttgg
    46381 taagtggtag tgcgatgcaa aacttttcca ttttctcaat gggggtttca ccatacatta
    46441 ctgcttctat cgtcattcag cttcttcaaa tggacatcgt gcctcgattt gttgaatggt
    46501 cgaaacaagg ggaagttggt cgtaaaaaac tgaatcaagc gactcgatat ctgacattag
    46561 tattggcatt tatccaatct gtcggtatca ctgctggatt caatcaatac gctcaattag
    46621 gtttcgtgaa taatcctaac gtagcaactt acgtaacgat cggtttgatt ctaacagccg
    46681 gtacgatgtt tgttacttgg ctaggagaac agatcacaga aaaaggtatc ggaaatggcg
    46741 tatcaatgat catttttgca ggtatcattt ctagattgcc tgaaagtatc agagaaattt
    46801 atgaagatta ctttataaat attgatcctt cagacatatg gaaatctgct atttttgtgg
    46861 ccattttact agtagcggtc ttgttgatcg tgacattcgt aacatttttc caacaagctg
    46921 aacgcaagat cccgatccaa tatacgaaac gcgtagctgg cgcgcctaca agcagctatc
    46981 tgccgttgaa agtaaatgca gctggagtta ttccagttat ctttgctagt tcgtttattg
    47041 ctacgccaaa tgctgtttta caagcattga gcggtagatt cggttctgaa aactggtaca
    47101 aagtgatgat ggagatcttt aactataata cagcacctgg tgctacgatt tatacgattc
    47161 tgatcgttgc cttcactttc ttctacgcct ttgttcaagt aaaccctgag aaattagcgg
    47221 aaaacttgca aaagcaagga agttatatac caagcgtacg ccctggtaag gggactgaag
    47281 agtatgtctc aagattactg atgcgtttaa gtacagttgg tgcagtcttc cttggtttag
    47341 tggcattgct tccaatcatt gctcaaatgg tttgggatct tccacaatca atcggattag
    47401 gcggtacaag cttactgatc gttatcggtg tagctttaga aactgcgaaa cagttagaag
    47461 gcttaatgct gaaacgtaaa tatgttggat tcattgagta agaaagcgtg ttgaggtttc
    47521 ccctcaacac acgttctcac acatttatta ggagggaaaa caatgaacct cattttaatg
    47581 gggctgccag gcgcaggtaa aggaacacaa gctgaaaaaa tcattgatgc ttatggtatt
    47641 ccgcacatct caacaggaga tatgttccgc gcagcgatgc aaaacgaaac agctcttggc
    47701 ctggaagcaa agtcttatat ggataaaggt gcattagttc ctgatgaagt aacaaatgga
    47761 atcgtgaaag agcgactagc tgaaccagat acagagaaag gcttcttatt agatggcttt
    47821 ccacgtacgc tagatcaagc aaaagctctg gacgcaatgc taaaagattt gaataaaaaa
    47881 attgatgctg tcatcgatat ccatgttggt gaagaaatct tggtcgaacg tctagccggc
    47941 cgatttatct gcagcaattg tggagcaact taccataaaa ttttcaaccc tacaaaagtt
    48001 gaagacacat gtgatcgttg cggtgggcat gaattctatc aaagagaaga tgataaacct
    48061 gagacggtta aaaatcgtct ggctatcaac atcaaaaata gtgaaccaat cttggcttat
    48121 tataaagaac aaggtttgct aaacactatt gatggtgctc gtgaaatcga tgctgtattt
    48181 gcagatgtac aaaaaatcat tgaaaaatag cacgattgcc cgtagccgac ttgcattatg
    48241 cagttactct tgtcaagttc aataaattat gatagaatat gtcagtccga cttctagaga
    48301 atcctctaga tgatccattt tgagaatttt gaggtgggaa ctgcagtttc cgccatttta
    48361 tcgtgtgaaa atgcgaaaca agtacaagga ggtactgtgc gtggcaaaag aagatatgat
    48421 tgaagtcgaa ggtacagtcg tcgaaacttt gccgaatgca atgtttaaag ttgaactaga
    48481 aaacggacac caagttcttg ctactgtttc aggtaaaatt cgtatgcatt acatccgtat
    48541 cttacctggt gacaaagtaa cggttgaatt gtctccatac gatcttaacc gtggccgtat
    48601 tacttatcgc tttaaataat tgtactccgt aaaactacag gaggtattat catgaaagta
    48661 agaccatcag taaaaccaat gtgcgaacat tgtaaagtaa ttcgtcgtaa aggacgcgtt
    48721 atggtgattt gcccagcaaa tccaaaacat aaacaacgtc aaggataatt ggaggtgcta
    48781 taaatatggc tcgtattgca ggagtagata ttcctcgtga taaacgtgta gtagtttcac
    48841 ttacttatat ctatggtatt ggtaacacta ctgctaaaaa gatcttagct gatgctggtg
    48901 tatctgaaga tgttcgcgtt cgtgaattaa cgaatgaaca aacagatgct atccgtgcag
    48961 aagtagataa attaaaagtt gaaggggatc ttcgtcgtga agtgaacttg aacatcaaac
    49021 gtttgatgga aatcggttct taccgtggaa tccgtcatcg ccgtggattg cctgttcgtg
    49081 gacaaaacac gaaaaacaat gcacgtactc gtaaaggccc agctcgcgct atcgctggca
    49141 agaaaaaata attcttaagt gaaggaggtt aaaacttcat ggcagcaaaa aaagtgaatc
    49201 gtaaacgccg tgtgaagaag aatatagaag caggtattgc acatatccat tctacattca
    49261 ataacactat catcatgatc actgatactc acggaaatgc attagcgtgg tcatcagctg
    49321 gttcattagg tttcaaagga agcaaaaaat caactccttt tgccgctcaa atggcagcag
    49381 aaactgctac aaaagcagca atggaacacg gattaaagac tgttgaagta acagttaaag
    49441 gacctggttc tggacgtgaa gcagcaattc gttcattaca agctacaggt ttagaagtga
    49501 ctgcaattcg tgacgtgact ccagttcctc ataatggtgc ccgccctcca aaacgccgtc
    49561 gtgtttaatg agtgccgctc attttgagtt ttatcagtaa cgtcattgaa caagacactt
    49621 ttcgttttga aaggggtaaa gataagaatg attgagttcg aaaaaccaag aatcgcaaaa
    49681 attgatgaag aaaaagatta tggcaagttc atcgttgaac ctcttgaaag agggtatggg
    49741 actactttag ggaattccct acgtcgtatt ttattatctt ctttgccagg tgctgccatc
    49801 actaatattc aaatcgatgg tgtactgcat gaattctcta ccatcaaagg tgttagagaa
    49861 gacgtcactc aaatcatctt gaacattaaa ggcttagcgc tgaaaatgta tggccaagaa
    49921 gaaaaaaccc ttgaaatcga tattaccgga cctgctacag taactgctgg cgatattatc
    49981 gttgatagtg atgtagaaat tcttaacaaa gatatgtaca tctgtagtgt ctctgaagga
    50041 gctacgttcc atgctcgctt aacagtgaaa cctggacgtg gttatgttca agcagatgaa
    50101 aataaaaaag aagatatgcc aattggcgtt cttccagtcg attcaatcta cacacctgtt
    50161 cgtcgtgtga actatcaagt tgagaacaca cgtgttggac accgtgaaga ttttgataaa
    50221 ttgacgatgg agatttggac agatggttca attgaaccgt tggaagcaat gagtttgtct
    50281 gcgaaaatca tgactgaaca tttggatatt tttgtgaact taactgatga agcaaaaaat
    50341 gctgaaatca tggtcgaaaa agaagaaaca caaaaagaaa aaatgttgga aatgacgatc
    50401 gaagagttag acttgtctgt tcgctcttac aattgtttga aacgtgcagg aatcaatact
    50461 gtacaagaac ttacaaataa atctgagcct gaaatgatca aagtccgtaa cttaggacgt
    50521 aaatcattag aagaagttaa agcaaaattg cacgacttag gtttaggttt acgtaaagaa
    50581 gattaaaacc ttttacgaag gagggaaacc aacgtgagtt atcgtaaact aggacgcaca
    50641 tctagccaac gtaaagcgat gttgcgtgat ttaacaactg atttattaat caacgaacgt
    50701 attgtgacta ctgaagctcg tgcgaaagaa gttcgctcaa ctgcagaaaa aatgatcact
    50761 ttaggtaaac gcggagattt gcatgcacgt cgtcaagctg cggctttcgt acgtaatgaa
    50821 gttgccagcg tgcgtgaaga aaatgaggac atcgtcattg aatcagcttt acaaaaactg
    50881 ttcaacgata ttgcacctcg ttacgcagaa cgtcaaggcg gatacacacg cattttgaag
    50941 acagaaccaa gacgcggtga tggtgcacca atggttgtct tagaacttgt ctaaaatcac
    51001 actaataaca tcactttata gatgattctt ttagagtgtt atgatgttgg aagatatttt
    51061 tccgagtcta gctcagcgct gctcctcaag cgaatatcgc ttggggagta agcactcatc
    51121 ataaagtgtt tttttatacc ttcaattacc tgtcatgaca gatatgtttg aaggcataaa
    51181 aaaacatttt aagcgagaaa taattgaaaa ttttgtcttt ttttgatagt gttagtagga
    51241 actttaggat gaagaaggag taatcatgga gccaattatt gaattagaaa agataaatta
    51301 taaataccag ccagatgatc ttcgccccgc attgaaagat gtctccttta caattgataa
    51361 aggtgagtgg atcgctatta ttggacacaa tggttcagga aaatcgacac ttgccaaaac
    51421 gatcaatgga ttgcttctgc cagaatcagg gatcgtgaaa gtgggaaatc aaatattgga
    51481 tgaagaaaat atttggacga tccgacagat ggttggaatg gtttttcaaa atccagacaa
    51541 tcagtttgtt ggttcgacag tagaagatga cgttgctttt ggtcttgaaa atcaagggat
    51601 tcccagagaa gaaatgctag tgcgtgtgaa agatgcactt gagaaagtta gaatggctga
    51661 gtttgcttca cgcgagcctg ctcgtttatc aggagggcaa aaacaacgtg ttgccattgc
    51721 cggcgtggtt gccttacgac ctgatatcat tattttagat gaagcaacca gtatgctaga
    51781 tccagaaggt cgtgaagaag tgatctctac gatcaagaag atcaaagaag aaagtcaatt
    51841 gaccgttata tctatcactc atgatattga tgaagcagct aatgccaatc ggatactagt
    51901 gatgagacaa ggcgaacttg tccgtgaagg tacaccaaaa gaaatttttt ctgctggtcc
    51961 tgaactgatc gacttaggtc tagatctacc atttcctgaa aaactgaaga gcgcactgaa
    52021 agaacgtggg gttgatgtgc ccagtgaata tatgacagaa gaaaggatgg tggattggtt
    52081 atggacatcc gttttgaaca agtagacttc acttatcagc cgaatacacc atttgaacaa
    52141 agagccttat ttgacatcaa tatgacgatc aaagaaaaca gttatactgc attagtaggg
    52201 cataccggaa gcgggaaatc aaccttactt cagcatttga atgcgcttgt taagccaaca
    52261 agcggtacag tccatattgg agaacgagat attcagccgg atacggataa taagaattta
    52321 aagcccatcc gaaaaaaagt tggcatcgtc tttcagtttc cagaagcaca gctattcgaa
    52381 gaaacggtag caaaagacat tgctttcggt cctaaaaact ttggtgtcag cgaagaagaa
    52441 gcactagtcc tagcaaaaga aacattagaa caagttgggc tggatgaaag ctatttggaa
    52501 cgttcgccat ttgaactttc aggagggcaa atgcgtagag tagcaatcgc tggtgtgctt
    52561 gccatgaggc cggaagtact tgtattggat gaaccaacgg caggacttga tccccagggt
    52621 agaaaagaaa tgatggagat gttttggcgt cttcataagg aacagcagat cactattgta
    52681 ttagttacgc atttaatgga tgatgtagcc aattttgcag attatgtcta tgtgttggaa
    52741 aagggacgaa tcgttaatag cggggagccg caagaggtct ttcaaaacat tgaatggcta
    52801 aaagaaaaac aactaggcgt acctactgcc accgagtttg cagaagaatt aatggctaaa
    52861 gggatgaact ttgctacctt gcctttgaca gctgaagaat tagcagacgc tatcgtccat
    52921 tatgctggag gaaatgccca tgatgaataa attgatattt ggtcgttata tgcctggaga
    52981 ttccttgatc catcgattgg atccccgagc taaattatta gccagttttt attttatcgg
    53041 aatcattttt ttagcaaata attgggaaac gtaccttttg ttggcatttt tcacattact
    53101 ttctgtcttc ttgtcaaaag tagatttgaa attttttatc cgaggcattc gcccattgat
    53161 ctggctaatc ctcttcactg tagctttgca ggtcttgttt acaagtggcg gagaaactta
    53221 ttggagttgg ggtgttttcc atatcactga atttggtgtg atgaacggcc tctttatttt
    53281 ctgtcgattc gtgttgatca tttttatgtc gactctactc acgcttacta caccgccttt
    53341 ggaattatcg gatgcgattg agtacttgct tcgtccattg aaagccgtac ggttcccagt
    53401 ccacgaaatt tcattgatgc tgtcgattgc tcttcgtttt gtgcctacat tgatggacga
    53461 aacagaaaag atcatgaacg ctcagcgcgc tcgcggtgtt gattttggtg aaggaaacct
    53521 agttcagaaa atgaaagcag tcattccttt gctgattcct ttatttgtca gcagcttcaa
    53581 ccgtgcggaa gatcttgcga ctgctatgga agcaagaggc tacaaaggtg gggaaggaag
    53641 aacgaaatat cgtgtattgc attggcataa tagagacacg atggttatca ttgcttttgt
    53701 attattaact gtgctactga tctttttgag aggataaacg ttcagtgggc cgctatctat
    53761 tcttttcttg ctgatctgtt tgcaagaaag gatgcgagca gtgtatgaca agaaatatta
    53821 gggtctgtgg cagactaagg tcacaggcct ctttctttta tctgaaaaga aagaacgaaa
    53881 aaattttata aaaaaatcat tattccagat aaccttttag gaacaaagga gagaaaaaca
    53941 tggtacgtta caaagcaatc attgcatacg acggaacaaa ttttaatgga ttccaaaaac
    54001 agccaaatgg gcgtactgtt caagaagaag tagaaaaaac attacaaaaa atggcaaatg
    54061 gaaaagaaat cacggtcttt ggttcgggaa ggacagatgc cggagtacat gcgattgggc
    54121 aagtaatcca ttttgattat ccagaggagc gtccgttaga acgaatgcgt ttcgcgctag
    54181 atacccagtc accagaggat attgctgtaa gacaagtaga aattgtttca gaagacttcc
    54241 acgcaagata tttggtcaaa gaaaagacgt atcaatttcg agtggatata gggaaaccac
    54301 gcagtccttt tcgacgccat tatgcaagtt attttcctta ccctcttgat ttggaaaaaa
    54361 ttcagcgggc gcttcctgat ttattaggga ctcacgattt cacctctttt tgtgcatcag
    54421 gaagctcgat agaggataaa gtacgtacga tctacgaggc taagatggag gtaaatgaaa
    54481 caaaagacga attgttgttt acatttaggg gaaatggctt cttgtataag atgatacgga
    54541 tcttagtagg aacgcttttg aaaattggca atggccgatt gcctgaagat agtatcccag
    54601 atattattgc gaaaaaagat cgtaatgcag caggacctac agcacatcct gaaggacttt
    54661 atttgtatga agtgaaatat gaataagagg ttgtgacaga agtggtcagc ttcaagaaat
    54721 aagaaggaat ttacgaaaat tacttctcaa aattttgcaa aatttcggct tattttcgaa
    54781 ggagctactt ctggaacacc gtgaatcaga ggttgtgaaa aagctgccct gcatcaagta
    54841 ataagaacaa ccaaacaaaa atagctcgtc atatttttcg tttggttggg cttattaccg
    54901 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaatcagcg ttttgacctg
    54961 agtattagag aaacaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
    55021 ccgaaggtca gctgattgaa caccgtttaa gaaagaggtc gtgagcctgt catttaactc
    55081 tgagtcacaa gtaacaattt caaaaaacag cttctcatct ttgatgaata tcgtgacttg
    55141 tggacgaggc gttgataagt gaatcatctt tttgttacct attttttctg taaccaaaaa
    55201 acgatggaac gttgttttga ttgcccttaa tgaggacagt cggaacaggt ttgtcatttt
    55261 tatatttaaa tggcacaaat ttttctaatt atttgtcatt tagcgctgaa aatttttgct
    55321 atttatctaa aaaagtgata actacttaag atgaagcaga atatttacgc catacggcat
    55381 acaaccgcga aaaatataaa aaaaagaaaa cgattgaacg cgtttttgca gtcgcaaagg
    55441 aaaagcatgg aatgcgctac atcaagtatc gagacttgga aaacaatcag gtgtatacgc
    55501 tgattgtttt tgctgcaatg aatctgaaaa agttgtccac ttggcagttg gaacatatgt
    55561 cttcatatct tttattatac aaaaatagca aaagcaccag tctacactct tagtaaagag
    55621 gaaactgatc cttttgtcta cattcttaaa gtacttaaac taagtacttt attttactct
    55681 ttaggattga tacagtagca tctatccctt tattaataat cttttatttt tttaaataaa
    55741 gatgttgtct ccatcttcag gcactaaaac tttttcggaa attccttttt ctttattgaa
    55801 agactttaat tcttctcttg acagtcccca atggtttaca gcttccatat gaacggcaat
    55861 aattgtggaa tctggtattg ctttatgcaa ttcatataaa tcgtctttcc ccataataag
    55921 agattctcca actaaaaaat gattatctcc agcatttgct actatgattt ctggattata
    55981 ctgatcaata gtttgtttaa cagcttcata ccaaactgta tctcctgtaa agtatagtgt
    56041 cttctcagtc gaatgtttga aaacaatacc gcaaacatta cctacataat tcaagatttc
    56101 tcctctgcca tgtttgcctt ttgttttaac caattgtata gattcaaaaa ctgtgtcttc
    56161 tcgtagtacc tccacatttt taaaaccata tagtcgaatt tcattcgcat cattttcatc
    56221 ttgcacaaat aattttattt ctttaggtag gacctctttc gctatatcgt cccaatgatc
    56281 tagatgtaaa tgtgttacaa taacagcatc aataccgttg ataatatctt cgactggcat
    56341 tggcaaatca actagaggat tcatttgatc ttttcttggt gatggaaaag gacctttcga
    56401 aataaatggt ggaagtgttc ctttttccgc taacattgga tcaattaaaa attttttacc
    56461 tccatagtct aagatagctg ttgcattacg aatgtgatgt atattcataa acgttgtctc
    56521 ctttttgttg atatgaaata tttttagtat aattcaattc catacaataa ataaaccgaa
    56581 caaaaaaagt cttttactat ttttacttaa tttatgaaag gttgtttacc atgttagacc
    56641 aaacagatat aaatatcatt gaggaactat caaaaaatag tcgaatcaca atgaaagaac
    56701 ttggagaaaa agtacatttg actggacccg ctgtctcggc gagagtgaca aaactagagg
    56761 aaagtggagt tattgaaaat tatacagtaa aattaaatat ggaaaagcta gggttttcga
    56821 ttcacgcttt tttaattata attacgcaaa atttaaatca taaaccctat ttatcattta
    56881 ttaaaa
//
LOCUS       DAJSKS010000004        50412 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJSKS010000004 DAJSKS010000000
VERSION     DAJSKS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.004"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..382
                     /locus_tag="ON396_000224"
     CDS             <1..382
                     /locus_tag="ON396_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HCR3112361.1"
                     /translation="IEKDAISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNI
                     EIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMK
                     KADYVTVSNDKDGVSQAFERILTI"
     gene            663..1883
                     /locus_tag="ON396_000225"
     CDS             663..1883
                     /locus_tag="ON396_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HCR3112362.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1880..4600
                     /locus_tag="ON396_000226"
     CDS             1880..4600
                     /locus_tag="ON396_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3112363.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4584..5528
                     /locus_tag="ON396_000227"
     CDS             4584..5528
                     /locus_tag="ON396_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3112364.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5597..6607)
                     /locus_tag="ON396_000228"
     CDS             complement(5597..6607)
                     /locus_tag="ON396_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3112365.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6787..7131)
                     /locus_tag="ON396_000229"
     CDS             complement(6787..7131)
                     /locus_tag="ON396_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR3112366.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7124..7558)
                     /locus_tag="ON396_000230"
     CDS             complement(7124..7558)
                     /locus_tag="ON396_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCR3112367.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7696..8433
                     /locus_tag="ON396_000231"
     CDS             7696..8433
                     /locus_tag="ON396_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3112368.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8426..9637
                     /locus_tag="ON396_000232"
     CDS             8426..9637
                     /locus_tag="ON396_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3112369.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9841..10623
                     /locus_tag="ON396_000233"
     CDS             9841..10623
                     /locus_tag="ON396_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCR3112370.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10693..11355
                     /gene="trmB"
                     /locus_tag="ON396_000234"
     CDS             10693..11355
                     /gene="trmB"
                     /locus_tag="ON396_000234"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR3112371.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11453..13207)
                     /gene="ade"
                     /locus_tag="ON396_000235"
     CDS             complement(11453..13207)
                     /gene="ade"
                     /locus_tag="ON396_000235"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR3112372.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13339..14268
                     /gene="tadA"
                     /locus_tag="ON396_000236"
     CDS             13339..14268
                     /gene="tadA"
                     /locus_tag="ON396_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCR3112373.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14225..15274
                     /locus_tag="ON396_000237"
     CDS             14225..15274
                     /locus_tag="ON396_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCR3112374.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVSA
                     AVLLPVYGSIEEVLP"
     gene            15271..15549
                     /locus_tag="ON396_000238"
     CDS             15271..15549
                     /locus_tag="ON396_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR3112375.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            15524..15994
                     /locus_tag="ON396_000239"
     CDS             15524..15994
                     /locus_tag="ON396_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCR3112376.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16011..16313
                     /locus_tag="ON396_000240"
     CDS             16011..16313
                     /locus_tag="ON396_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112377.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16330..16701
                     /locus_tag="ON396_000241"
     CDS             16330..16701
                     /locus_tag="ON396_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HCR3112378.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            16673..17032
                     /locus_tag="ON396_000242"
     CDS             16673..17032
                     /locus_tag="ON396_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112379.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17204..18211
                     /locus_tag="ON396_000243"
     CDS             17204..18211
                     /locus_tag="ON396_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3112380.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18236..19420
                     /locus_tag="ON396_000244"
     CDS             18236..19420
                     /locus_tag="ON396_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR3112381.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19857..21440)
                     /locus_tag="ON396_000245"
     CDS             complement(19857..21440)
                     /locus_tag="ON396_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR3112382.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22018..22161)
                     /locus_tag="ON396_000246"
     CDS             complement(22018..22161)
                     /locus_tag="ON396_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR3112383.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22383..22862)
                     /locus_tag="ON396_000247"
     CDS             complement(22383..22862)
                     /locus_tag="ON396_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR3112384.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22935..23219)
                     /locus_tag="ON396_000248"
     CDS             complement(22935..23219)
                     /locus_tag="ON396_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112385.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23297..23491)
                     /gene="ssrS"
                     /locus_tag="ON396_000249"
     ncRNA           complement(23297..23491)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ON396_000249"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23563..24849)
                     /locus_tag="ON396_000250"
     CDS             complement(23563..24849)
                     /locus_tag="ON396_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCR3112386.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            24987..25658
                     /locus_tag="ON396_000251"
     CDS             24987..25658
                     /locus_tag="ON396_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCR3112387.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25624..26112
                     /locus_tag="ON396_000252"
     CDS             25624..26112
                     /locus_tag="ON396_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HCR3112388.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26125..27072
                     /gene="prmA"
                     /locus_tag="ON396_000253"
     CDS             26125..27072
                     /gene="prmA"
                     /locus_tag="ON396_000253"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR3112389.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27073..27837
                     /locus_tag="ON396_000254"
     CDS             27073..27837
                     /locus_tag="ON396_000254"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR3112390.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28070..30283
                     /locus_tag="ON396_000255"
     CDS             28070..30283
                     /locus_tag="ON396_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR3112391.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30294..30740
                     /locus_tag="ON396_000256"
     CDS             30294..30740
                     /locus_tag="ON396_000256"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR3112392.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30911..32170
                     /locus_tag="ON396_000257"
     CDS             30911..32170
                     /locus_tag="ON396_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HCR3112393.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32259..33206)
                     /locus_tag="ON396_000258"
     CDS             complement(32259..33206)
                     /locus_tag="ON396_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR3112394.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33600..34892
                     /locus_tag="ON396_000259"
     CDS             33600..34892
                     /locus_tag="ON396_000259"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR3112395.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34907..36676
                     /gene="aspS"
                     /locus_tag="ON396_000260"
     CDS             34907..36676
                     /gene="aspS"
                     /locus_tag="ON396_000260"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR3112396.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36785..37660
                     /locus_tag="ON396_000261"
     CDS             36785..37660
                     /locus_tag="ON396_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3112397.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37926..39809
                     /locus_tag="ON396_000262"
     CDS             37926..39809
                     /locus_tag="ON396_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR3112398.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39884..40168)
                     /locus_tag="ON396_000263"
     CDS             complement(39884..40168)
                     /locus_tag="ON396_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR3112399.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40158..40892)
                     /locus_tag="ON396_000264"
     CDS             complement(40158..40892)
                     /locus_tag="ON396_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCR3112400.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41021..41644
                     /locus_tag="ON396_000265"
     CDS             41021..41644
                     /locus_tag="ON396_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCR3112401.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41836..42957
                     /locus_tag="ON396_000266"
     CDS             41836..42957
                     /locus_tag="ON396_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCR3112402.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            42964..46092
                     /locus_tag="ON396_000267"
     CDS             42964..46092
                     /locus_tag="ON396_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCR3112403.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46111..49305
                     /locus_tag="ON396_000268"
     CDS             46111..49305
                     /locus_tag="ON396_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HCR3112404.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49451..50158)
                     /locus_tag="ON396_000269"
     CDS             complement(49451..50158)
                     /locus_tag="ON396_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR3112405.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16454 a         8859 c        10507 g        14592 t
ORIGIN      
        1 tatagaaaag gatgctatca gcatttacaa attttttatt tttattccta atcgtcaatt
       61 gaaagctaat atatttaacc atttgaaaaa gatgctaaat gtgtctgtta ctgaatcggc
      121 acctgtaaat attgaaatcg tacctcaaaa tgtatctaaa ggtgtggtgt ttgatcgttt
      181 ggaatcaatc tacaatttaa agaaaccttt gagaatcgcg atcggagata gtttaaatga
      241 cttatctatg tttgaaagtg cagatattag ttttgcaatg ggaaatagtc atcaagaaat
      301 catgaaaaaa gctgattatg tcactgtttc aaatgataaa gacggagtat ctcaagcatt
      361 tgaaagaatt cttaccatat aaactaatta aggtagtata gaacatagaa ttctaaatac
      421 ggaaaaatgg agcataaatt ccagtacaaa tagtacttct tcagtgtcaa acgaagcgaa
      481 tataacatag atatttagga ttgtgacaaa aatcatgtca caatcttttt ttcgaataaa
      541 cttatttttt gaagtcggcc atttatcgta atttctttgc ataatacgaa caaattttcg
      601 gttattgatg aaaaaaaaca ttgaaaatgg tatgataaaa gaaaaaaagg aggaaatttt
      661 taatggtgaa atttatccat gcggcagatt tacatatgga tcggtcattt gaaggactga
      721 caactcttga taaaactgta caggaaaaac tattgaagac gaatctgacc gttttatcaa
      781 atatcattga acaggcaata ataaataacg ttgattttgt tttacttgca ggagataatt
      841 ttcatcaaaa tcgtccttca ttaaaaatac agaaacattt ttcagaacag atgaaacggt
      901 tagaaaagaa ccatatacct gtatttataa tattcggaaa tcatgatttc tatcaaaaag
      961 agcggtattg gttctccttt cctaaaaatg tccatttatt tacatccgaa acggtagaga
     1021 caaaaaaaat aacgataaaa agtggagaaa ctgtctctct cagtggattt agttatcgtc
     1081 aaccgtggat tcaaaaagac aaagtgatgg aatttccttc tagagaactc acagattacc
     1141 atattgggtt gtaccacggg gaacctggcg tctctcaaaa aggaaactat gctcctttcc
     1201 aaccctcaaa gatgcaagaa aagggatacg attattgggc tttagggcat atccatgtgc
     1261 caacggtctt aaatgaaaag caaacaattg tttatccagg tgcgcctcaa ggtcatacta
     1321 aaaaggaaca agcttctaca tctattctac tagttgaatt aagtaaagga agttgccaaa
     1381 tacatccgat taaagtagca gaggtctatt ggaagacaaa ggaaatatca ttgaggaaag
     1441 caagaacgac aaaagaggtc attgttcaca ttcgtcagca gctttcaaaa gtagaggatg
     1501 gatttagttt aattgagata aaattaaaag attatgacca tctcaataca gatgttttgg
     1561 aacggataca atcaggagag ctattagact atttgttaga ggaattctca gataggataa
     1621 atgatttctt tatttggcgt atttctctca tagagaatac tttttatacg aagaccccac
     1681 ttgctgcttc tgctaaattg atggaacagt tatttcaata ctatcagaca cctcaagatt
     1741 tccaacaaat tttaaatgaa gtatatagtc atcaagaagc ggctagaata ttgagcgaac
     1801 tgccggaata tcaagaagaa acacttgaaa aagccaagca gctgcttaat caagattttt
     1861 tatttgagga ggatcaggaa tgaagatttt aaggatcgag attgctgctt ttggaaaatg
     1921 gcgtcaaaaa agttttgact tttattcagg caatcagctg atctatgggg gaaacgaagc
     1981 aggaaaatca acgatttatc aatttattca agcaatatta tttggtttcc cttcaaaagg
     2041 ccgtaaaaaa aaggattata caccaaaaga cggctcagcc tatggcggca aaatatggct
     2101 taaacatcct gtatatggtg aatttgctgt cgagcgctac aagcagcaaa atcgcggaaa
     2161 atcgaaggtg tggctggggg atcaagtggg aagtgatgag ttactagaaa aactgatcgc
     2221 acctttgacg aaagagttat tccagcaggt atttactttc cagcaagagc agttgatgga
     2281 actagatcat ttgagagaaa aagagctaca tgatgcgctg atttctttag gcatcacggg
     2341 cagttctcaa gtattccaaa agcggcaaga atattatcag aaagcacaac aattattcaa
     2401 gaaaaaaggc caaaaattac taatcaatca aaaattaaat gaatggaaag agctgcaaga
     2461 aaaaattcat caaaaacaag aacaggaagc ttattttggc aagttgatcc aacaagaaca
     2521 ggaatacgag tcgcaacagc agcagttatt ggcacaatta aaagaggcga acaaacaatt
     2581 ttcattagcc agacagcaag aacttaattt ttctttatat gaagaatggc aatctctcaa
     2641 aaaaaacagc actacaggtc ttcctgatga gcaacttctt ttagatctgg agtctttctc
     2701 caaacggtat cagcaaacaa atgagcgttt ggaagaatta gaagcaacac tggaaaagca
     2761 tagcgggatg gatcagcagt cagccagata ttatttttat ttagagcagg agcaggaact
     2821 aaaagatctc cagcaacttc aatttacagc ggaacaatta aacgaagaat ggcaaagatt
     2881 acaaattgag actagagaaa caaattcaga attgaagatt ttggaacaaa ggtggcattg
     2941 gcagaaaaat ccgttaccgg aagaattttt atatgaagaa gaatggaagc atcttctggc
     3001 tgaagaaaga aggattgctg atctagaaag ccagatttct gtgcgtctcc aaatagctaa
     3061 agaacagcaa gagatgattg aagcagaagt gaatgacttg gaagagacct atccagcatt
     3121 attacaaaat cagtcagaga tagcaccagt ctcttcatct aaaccgtata tgctgatggc
     3181 tggcggagca ataatcattg caggtatatt tgtaccaatg cccttgaaag tgatcctttt
     3241 actaattggt gcaggcatga ttggctattt cttctacaaa aggcaaccaa aaattaaaac
     3301 taaaaatgat ttctctgaga taaaaggaat gtggcaagaa aaactaggac aacttgatct
     3361 agccttagct caattagctg aagcagaaga aaaacaaaag agaactggag aagaaaaaca
     3421 gattcttcag caagaagtat atcgttttgc ggatcatcgg cacttaggaa agatggatac
     3481 gctcaaactc atgaaagaat atggacagga agtcatagat tatcagaaat tatatggcgt
     3541 atttgagcgg aaacataaaa gagaacagga aatacaagaa aaactacgac aaatcgacca
     3601 aaaatttgag tttgttaaag aatggttacc gatacaagat aagacaattt cagaaaaaat
     3661 ggtattgctt tcccgtttta tacaagagat ggaacagatg aaatttgcca gaagctatca
     3721 gcaaaatact ttattgaagc aagaaatcaa tcaattgaaa aagaaacaaa aagtatggat
     3781 acaagaatac agcgaacaat tgactcaagc aggcttatct tatccttcgg aagtacctgt
     3841 ctttttacaa aaagccaaac aagccgaaca gcagatgaaa agatacaatg aattatcgga
     3901 aatgcttact ccactatttc ctgaagagct gacaaaagag aaattatctg taagactgcg
     3961 ccaactgaaa gaaaagcaat cccaactcca gcaagaagaa aaagaagcaa gtgaaaatcg
     4021 gcaacgactc caacttcaag tagaagagtt gcaaagagac ggcacattgg acgaacttta
     4081 tcaacaagct agccggcaaa aagcaaaatt acaggaatta ctggaggaat ggggagcact
     4141 tgaaatcgcc gggacattct taggtgatct ttcaacagag atgagtgaaa gacaattacc
     4201 ccagttgtta agagcagcag gtgattattt aggtatttta acaaaaggaa attatcagaa
     4261 gatattactg aaaaatgata cactgacttt aacagacgga tatacagaat ttcccatcta
     4321 tgagttatct acaggtacga aagatcagct gattatggcg atgagatttg cttacctctc
     4381 attagaagct aaccgttcga tatgcccgat catcattgat gatggctggc ttcattatga
     4441 tcatcagcga aagtatcaat tagctgaact tttagctaaa tttggagaaa agtatcaaat
     4501 aatttgtttt tcttctgata aagaaatggt aagctattac catgagatga accaaatcgt
     4561 aaaacaatta gaaggagcaa ataatgaaaa aaattcgtga tttagtaatc gatgagcagt
     4621 ttgaacaatt tgtcctgatc aaaagtgcag atgtaagaat cgctaaaaac ggaaaaaaat
     4681 ttattgcttt tactttccaa gatacttcag gtactatcga tggtaaatac tgggatgctt
     4741 cggaggagga aatcaataaa tttacagccg gcacagtcgt ttttctaaac ggtaaaagag
     4801 aggtttatca aggaaaccct caagtaaaga ttcttcatat gcgcttaaca agggtagatg
     4861 agccaggaga gcctagttta tacatggaaa gagccccttt gaagaaagaa gaaatggaag
     4921 aagaaatcaa tcaattgttg tttgagatca ccaatgcgca ttggaatcgt attgttcgct
     4981 atctacttaa taaatatcaa aaacaatttt ttgagtttcc ggcagcaaaa agaaaccatc
     5041 atgcatttgc cagtggatta gcttatcata ctttgacaat gcttcactta gggaaatcaa
     5101 tcgccgatga atatcctgag ctgaatcgag cattgttgta tgctggtatc attttgcatg
     5161 atttaggaaa ggtgattgaa ctttccggtc ctatgtcgac agaatatacg cttgcaggaa
     5221 atttgatcgg tcatctggta ttgattgacg aagaaataac taaagcctgt cttgcgttga
     5281 aaatcagcga agctgacgaa gatgttcttg tattacgtca tatggtttta gcacaccatg
     5341 gcttattaga atatggctca cctgtacgtc caagaataat ggaagcagaa atattacatc
     5401 aaatcgacaa tatcgatgct tcgatgcaga tgatgttgac ttcgattcgt caaacagaac
     5461 caggaaaata caccgatcgg attttcggta tggacaaccg aagtttctat gtaccaaaag
     5521 atttatagca aaaatttatt tttttcattc aaaacaaaaa aagcgagaca taagtcttcg
     5581 ctttttttgt tttcttttat tttgatgaat cagttgtttc ttcagttgaa gattcttttg
     5641 ttgaatcagt tgattttgtg tcagaactct ttgttgaagc tgtagtttca cttgagtctt
     5701 tcgtacttga tgaagttgtt gtgaatgcac tcaacacatt ttcaaaggca tcatctttga
     5761 tttttacatt tgcatctttt aattcttcac cgattacttt agttgtgaat gcattgtcac
     5821 ttagtttagt ttcagtagcg atatctttta gttgatcttt gtatttatcc atgtcattgc
     5881 ctttattttg gtttttcacc attttgacaa cgtagtattc tgtcgcataa gaagtaggat
     5941 ttgttgtagt aataacatcg gagatctcac cgtctttcaa tttgaaagct gcttctttta
     6001 cttctgcagg aatagttgtt gtcgttgagt cgaatttaac tttaccgcca tcttcttttg
     6061 tctctgtatc agttgatttg tctttggcta atttagagaa atcatctcca tcatccgctg
     6121 attttttcac atcttttgct tcatcttcac ttgataattt gatgatttgt gcttctactt
     6181 ctggatggaa agatttccaa gcagttttca aatcgtcatc tgtaatatct acgtgagctt
     6241 tcaagccagc ttcaaaagct aaattgttac gaatatattc tttataagta tctttcgtat
     6301 atccagcagc ttctaattgt gattcaaaag tatcacctaa tgatttcgct tgtttgtcgt
     6361 attctgcatc cacttgctta tcggttactt tatcgccata tttgttattg aatactttat
     6421 aaatgatcat ccgttgtact aaggattggt tcgatgattc aagttttgct tcatcataga
     6481 aatcagatac agtgatcgtt ccgcctttca tcgttgcgat atctttatta gtatcgcctg
     6541 aacaagctgc tagagttaaa acagccaacg cactagtagc tgccaaaata attgattttt
     6601 ttttcattaa aatttgcact cctgtaatta gttattattt ttatatatta actataccat
     6661 atcccatttc ctttaaaaac gttgtcgtgg tagtttcaaa caaaattcaa aatttcatca
     6721 cattcctagg aaatacgcaa acttttctca cttatatagt taaatctgtt gagaaaataa
     6781 tcattattat atttgttcag gatactctgg cagatcagct tttagttttt ctacttgttc
     6841 attgatttgt gtgatacgtg gttcggcttg gaatttaaaa tcttcgatat cttttttgat
     6901 tgcttcttgg aaagcaggaa tcacagattc agctgtttct tttgtttcga tcaatgcgct
     6961 tttaaaatga ttcaggctgt tatttaaatc atttgtcaga gttgttgctt cttccaattc
     7021 ttcgatcagt ttctttcttg tttcattccc gctcctgggc gccaataaca gacctccgac
     7081 agtgccagca gcagctccaa ataacagtcc tttgaaaaag tttttaatca tcgcaggaca
     7141 cctgctttac gattgtttct tgaatagctt ttagttcttc cgatccataa ctatcttgat
     7201 gattgacaaa gtgaatagaa aaatcatcct ctttgctgta gcgagggact aaatggatat
     7261 gtgaatgaaa aacagattga taggctagtt cacggttgtt attcaagata ttaagacctt
     7321 tcatttctgg aaaagctttt tccaatgctt gagcaatctt aggcacacgc gaaaaaacct
     7381 ctcctgcaag ttcgggatca tattcaaaaa tatctgctac atgacgttta ggcacaacta
     7441 atgtgtgtcc tttcgttgct tgagagatgt ctaaaaaagc atagaccttg tcgtcttcat
     7501 atattttata gcttgggatt tcttgattga tgattttgca aaaaatacag ttttccatat
     7561 aaaacatcct ttctataaag aataaatact tcagcttaac tttaccataa gttaaagcta
     7621 ttgtaaggaa aaaaacaaaa taccgtcatt ttcttcgtgg tataataaat aaaaataaat
     7681 ggcggaggaa aaagaatgag tttaattatc gatcatgtca caggggggta cggacatctc
     7741 cctgtgttaa aagatatcag tttcgatgta cagcctggag agatagtagg gttgattgga
     7801 ctgaacggag cagggaaaag tacgaccatc aagcacatta tcggtctgct ggaagcttca
     7861 aaaggaacga tagaaatcaa tcatctatcc ttaaagaaaa atacggaaga atatcgcaaa
     7921 caaattgggt atattccaga gactccttct ttatatgaag aattgacatt gagagaacat
     7981 atcgaagtca ctgcgatggc ctacgatata cctatggata tagcaatgaa aagagcagaa
     8041 aagttgttgg agacttttcg gttatctaat cgtcttgact ggttcccagc taatttttca
     8101 aagggaatga aacagaaagt gatgatctta tgcgcctttt tgatcgagcc ttctttatat
     8161 atcatcgacg aaccattttt aggcttggat ccactagcta tcaatgcatt gcttgaattg
     8221 atggtagaaa tgaaaaaaga aggagccggt attttgatgt ctacgcatat tttagctact
     8281 gcagaaaaat attgtgatcg ttttgtggtc ttacataacg gagagatacg agcagacggt
     8341 tctcttgaga tgttgagaaa agaatttcag ctgccggatt cttctttgga cgaaatctat
     8401 attgctttaa caaaggagca ggcacatgag tagttttttt caaaaacgtt tagcaagaca
     8461 tcaaaagaaa atgatgaaat atatgcgtta tgtattgaac gatcattttg ttttagtttg
     8521 tcttttcttg attggcggtg tgggattata ttattctaat tggttgaaaa ccctgcagcc
     8581 accgtttgca attggagggc tcataatctc tgtattttgg atgctctgtt tattcgtggg
     8641 taagatggct acttttgcag aggcagcaga tattgttttt ttacttccta aagaaaaaga
     8701 aatgcaaacc tatttggaac gagcttttcg ctattcttgc atttttccgt tgatcttttt
     8761 gatattggca tgcggattgg ccatgccatt agtggtggtt agtacggagc aatccttttc
     8821 gatgtttttc gtctatctga tcatattatg gagtttgaaa ttcagtcatc tgcaaatcaa
     8881 gagagcagaa ttgtttcgac tagatcaaca agttattcgt tattggaaat taggctggtt
     8941 tttttcttct ctgatctttt tgtttctatc attttattgg ctaatatggg caggaatagc
     9001 aggtgcatta gtacaggcaa gtctatatta ctggtttttg tggaagcaac aagaaaaagg
     9061 attggattgg gagaaaatgg tgcatgtaga agaacatagg ttgcatcaga tctatcaatt
     9121 tatcaattta ttcacagatg ttcctgaaat tacggcaaag atcaaaagaa gacgctattt
     9181 ggatcctata ttgactcata ttaaaagaga atcaaaaaac acttatcttt acttgtttgc
     9241 aagaagattg gcaagaggtt ctgattttag tggtctttat ttccgattag taattatagg
     9301 tgggttattg attgccggag tagtcgactt ttattttagt ctgattatcg gtttcttatt
     9361 tttatatttg atcggtttcc aactgttacc tttagcgaac cagttccgct atatggttct
     9421 cgttcagctt tatccaatcc ataagaatca gaagaagcag gcgattcagt atctactcac
     9481 atgggtatta attatcgcgt cggttatttt tggtttgatt gctgcagtcg tattagatgg
     9541 gcagcagcga tgggttccgc tagcggctta tttgattgtt actatgctct ttgttaaact
     9601 atatgtacca actcgtttga agaagatgtc agactgaaaa caaagctgtg tagggtaaaa
     9661 atgagaatga aaaatagagc gttgacagta agaatgctgt tcgttctatt ttttttgaga
     9721 aaagctggtt ttttgaatgg caattaaatg aaaaagaacg tatatgtgct tgacgtgtag
     9781 tgttcaggcg actagaataa taagtactgc aaaacaaaga gtaagaggac gggagaatgc
     9841 atgatggagt ttcagttaga ccaagagtgg cggttacgtc ccatcaaagg cgacacagga
     9901 aaaacataca taggcatgcg tgatgaggac aaagtcttta ttaaacgaaa cacgactccg
     9961 atgttggctg ctctttcaag ggaaggaatt acaccaaaac ttgtttggac aaaacgaacg
    10021 gggaatggcg atactttgac agcacaagag tggctggaag gtcgcttgct gactcctaat
    10081 gagattggtc aaagaaacga cgtgattgat gttttgtacc atttacatca ttcaaattct
    10141 cttaaaacca tgctgacccg tatcggtggt cgtgtcatga cgccggagaa gatgctgcaa
    10201 aaatatgaaa aagaattacc ggaagaactc cagaaaaatc aatttctgca aattgtccat
    10261 cgttttcttc gtgagcagat cccgtcaata gataaggaaa acttatcagt agtacacgga
    10321 gatgtaaacc atcgtaattg gttagtatgt aaaaattatt tatatctagt cgattgggat
    10381 tcaatcatgt ttgcagaccc tgcggtggat attgcaacga ttttaggaaa ttatgtgcca
    10441 ctgtctagct ggaatcagtg gctgatcagt tatggcatac gcccgactga cgaaatgctt
    10501 gaaaaatttc attggtatgc ggccatgaat ttgctccaag aaatcaccag atattgcaaa
    10561 cgtgaagatc atcgacggat gaacgaagaa attttacaat taaaacgaat tttcagtggc
    10621 tgaggtttgg cccaaactct gcggagattt ggccagacct ttttaattag aggaaaggaa
    10681 tggagataaa taatgcgtgt ccgaaatcgt ccgggagcag cggaaatgtt agcggctcac
    10741 ccgaattttg taattagtga tccaacattg tggaagggaa aatggaatga attgtttgaa
    10801 aacgatcatc caatccacat tgagattggt atggggaaag gccagttcat cactggaatg
    10861 gcaaaagccc atcctgaaat caattatatt ggagtagaaa tgcaggtcag tgttgtttcg
    10921 attgcactag acaagttgat cgaacaaccg cttcctaacc tgaaacttct acatgtggat
    10981 ggatcagcgt tgacagagta tttcgctgac agcgaagtag atcaaatcta tctgaatttt
    11041 tccgatccat ggccaaaaaa acgtcatgaa aaacggcgat tgacatataa gacttttctt
    11101 gctgtagatg aacagattct tcgtccaaat ggagaaatcc atttcaaaac agataatcaa
    11161 ggattgtttg agtattctct ggcaagtttt tctcaatatg gcatgatcct caaacaggta
    11221 tggttagatt tacatcaaag tcaatttgaa ggcaatatta tgacggagta tgaagaaaaa
    11281 ttttcttcaa aaggtcaacg tatttatcga gtagaagctc aatttcaaga taagaccaaa
    11341 aagaaaagtc tgtgaaacta actttctgct ttcataaaaa cattaatatc cataaataaa
    11401 tctcagaaaa tgaacattac agatgtaatg tattttctgg gatttatttg atttaaaatg
    11461 tatttatttc atcggttttt ccacaaggtg gaatcaattg gaagcgagaa aatgaaaata
    11521 atcctttgtc ggtgatcttc aattcgggta tgacagataa tgttaagaaa gaaagtgtca
    11581 aaaaaggatc aaaggtttta gctgctccta gttgatgtgc tttagttatt aattcattca
    11641 actgttcata gacgatcgaa aaaggttcct gtgtcatgat tccgcctata ggaagagaaa
    11701 cacaagctat ttcctgctgt ttgttgacaa cggtcatccc gccacctttt tgaatcaaaa
    11761 gattagcagc atacagcata tcctcgtcgt ttgtcccaac gacaactaga ttatgcgagt
    11821 catgggcaat cgttgtcgct atcgctcctt gtttcagctg aaatcctttc actatgccta
    11881 atccaatgtt tcccgtttga tggtgacgct cgatcaccgc aatctttaat tggtcttttt
    11941 cgattgaagg aatgaatcgt tcgccttgtc ggtccacttt ttctaccaaa tctttagtca
    12001 ctagactatt cgggataatt tcgatcacat gggcttgtga agaagaaaga agcagctcta
    12061 acgaatgtgg atttaatggt tgagcgttta gttttggcag acaacacgta tatgaatttg
    12121 aatgatcagc aaataaatgg tcttggagtt gcccagattg aacaactaat gttcccttat
    12181 agaaaacttc atgaattggc agcttggtaa gatcgtctgt taagaagaaa tcagcttgat
    12241 acccaggggc aatcgcacct agtgttttca aatcaaaaca ttctgctgca ttcaatgttg
    12301 ccatctgaat ggcttgtaat ggatcgattc caagtgcaat agcttgacgg acgatatgat
    12361 cgattgatcc ttcttttact aaatcattga tcagtttatc atcggtgaca aagaagcatc
    12421 ttcttgaatt ttcaggtgtg atcgccggta gtagcgatgc cagatctttt gcaacggtcc
    12481 cttctcgaac cattaaatac ataccaagat ccaaacgttc tttagcttct tttgcagttg
    12541 tagcttcatg atccgtccga atacctgctg caggataaac atttaactct tccatgccga
    12601 taccagctgc atgcccgtca atttttttct tatgctggtg catcaattgg atcttttcta
    12661 ggatgtctgt ttcattattt gctactgctt gatagttcat gacttcagct aagcctaaaa
    12721 cttcgggttt ttgtaaaaaa ggggctaatt tttttgcatt taaatttgca ccattggtct
    12781 caaaaggggt tacaggtaca caagaaggta gcataaaaaa gatattcatg agtgtttgtc
    12841 ttgcatcttc tatcataaac tcaagacctg tactcccagc aatgtttcct atttcatggg
    12901 gatctgtcac tacagtagta actccatgaa gcaaggatac ttttgaaaat tcgcttggtg
    12961 ataaaagtga gctttcgata tgcacatggc catcgatgaa tcctggcact agatattgcc
    13021 cattagcatt atgtacgctt tttccttcgt attcaccgat tcctacgatc attccttcgc
    13081 agattgctac atctttttcc atgatttctt tcgtaaagac gttgacgatc ttagcatgct
    13141 tgatcactag gtcagcaggc ttttttttac ttgctacttc gatatatttc cttaatgttg
    13201 tttccaatgg agtgcctccc aagataaatt tttataagta taacataaat tcggcttttg
    13261 ccaatatttt tttgtaaatt cattttattt gaaacaaaaa gatcaaaaag acgtatgttt
    13321 tttgtggagg ggagaataat ggaaataaaa cgattatcgg atcaaatgat aagtgaaagt
    13381 tttgccaaac atgcccaaga cgtatatata ttgccagctt cagaagtatt ttacatctat
    13441 tttcgctatg gaaaaaaacg aacattctgg aagaaagtct cttcggcaga tggacagcaa
    13501 ctgatctcaa gatttaagta tttgggacag atggatatcg gagaaaaaag aaaagctcag
    13561 ttaggtgcat ttacttattc tttagaaaaa caggaagttc gtctgcggtt gtcttctgta
    13621 ggagattacc ttcaacgaga gagtttagtg attcggctat tatacggtac ttttgaaaca
    13681 ccccatcgtt gctttagttc tcgagatttt gcaactattt tacagaaaac cgtcagacgt
    13741 ggattatacc ttttcagcgg accagtcgga tcaggcaaga cttctctaat gtatcggttg
    13801 gctttggaag aaaaattgca agtgattact atcgaagatc ctgtagagat agaagaaccg
    13861 ttttttctgc agctacagat caatccgaaa atagcacaga cttatgatac acttttgaaa
    13921 ctcgcattac ggcatcgtcc agacttgcta atcattggcg agataagaga tgaatcaact
    13981 gcacatgctg cggtaagagc agcactaaca gggcaccggg tttttgcaac cgtacatgca
    14041 cgcaatcttg aaggtacatt ggtcagaatc catgaactga caaagaaaaa agaagagcta
    14101 tctgaatgtt tagctggtgt catttatcaa gaacttttgg aagatgccaa tcaccatcct
    14161 tctgttttat ggagttatca atttatggag gaagaatcag taaaaaaatc atgggaggaa
    14221 agttatgaag aagctcagga aaatatttgg cagtcagaac tattctaaag gattaacaag
    14281 aagccagcaa aaccagtttg cacaaaccat gggagaactg cttttatctg gttttagtct
    14341 gcaagaatcc ctttatttat tactcacgat ccgttcgttt ccacctgtta ttttggcaaa
    14401 tatccaacat cagctaaagg aagggcaagc gtttagtgag gtactttcgc aaataggata
    14461 tggagcagag caactggtac agatcgagtt agctgaacgt catgggaatc tgccgacaac
    14521 tttgttggaa attgccaaac agtttcggct tgttgaagga tttcgcaatg atttgaaaaa
    14581 gctgcttgca tatccgatgc tattgctcac ctttttgcta ggtatcttgt ttagtatgcg
    14641 gttgttcgtt ttgcctcagc ttattggttc aggaatggtc atgcaagagc attggggtat
    14701 tcagctgata caagtctttc attggtatgt tctactattc atcctagcgg ttactctatt
    14761 ggctttacta gtaaggtatc gtctgatgaa gttgtctcct gttgatcaga gtgaatggat
    14821 aagcagacaa ttattgattg gaccgatata ttccttgtat caatcggctt atttgtcact
    14881 agagattgga aagctttttc atgaaggttt cgaattaagg caaatcatag tctgcttaaa
    14941 ggaaacgaaa caagaaagtc tgattcagca tcttgcctct aagttgattc aaggattgga
    15001 ggcaggtatg tcattggctg agcagttcca gaattatcgt tttctatcag aagaattcag
    15061 taaaattgtt ttacagggag aggcaaaggg aagtttaggt aaagaactga ttttttacag
    15121 cgaatggaca agaaaacagc ttttcaaacg tcttcatcac tggatgcatt ttattcagcc
    15181 aatcattttt ttgggcgtag cggctttgat tttattagtt tctgccgctg ttttattacc
    15241 tgtttacgga agcatagagg aggttttgcc atgaaaaaga gaaaaatatc ttattatagc
    15301 ggttttacat tgatcgaaat gctaatcgtc ttactgatca tcagtgtatt agtgttgttg
    15361 ttcgtcccta atttgtcgcg ttataggaat catgtagacc aagaaagtcg tgaagcgatc
    15421 atccagttgg tagacacaca aaaagagctc tatgcgcttc aaaataatgg aagagttcct
    15481 actgtggagg aattgttgaa tgaaggctat atcaaacgag aacatgcaga aatctatcag
    15541 cggccttgaa ggtaaggttt tagcatcagg atatacgttg tttgaatcac tcttactgat
    15601 tttattactt acaacgatcc tttcttttcc agttctcgca ttttctgcat ggaagaatga
    15661 attggcagtc tatcaatttt tcggccaatt tgaaaaacga atctattcta cgcaaaaaat
    15721 cgctattgtg aatcagatcc aaacaggctt ttattggaat agtgaagaga atcaaatcat
    15781 ttttcatgtt ccgaatccag ataaaataaa ttgggaagta ttagatattc ctgatgagat
    15841 taccttgaaa cgccatgctt ctattacttt tgctgctaga accgggaatg aaagttcact
    15901 aaaagcttat caattttatt gggaagaaaa agagcggacg attacgtatc aattccagat
    15961 agggagtggt cgctatacaa aaagaattga ataaaacagg atttgttttg ttggagtccc
    16021 ttacagcgct tatgatcgct tctgtctgtg tctacttagt gagctttggc cagctgtttt
    16081 tgcttcaggc agaaaagaaa agtggggagg aactgctatt tattcgaatg atttatgaag
    16141 atatcaagac gcagcggatg tatcacaaaa gaccagagcg aagggaagaa atgatcccat
    16201 acgatatcca aattgtcgat aataatggag taatcgaaaa agtgtttgtc aatatggaga
    16261 aagaagcgat cagtattgaa aaaacaagta attccagcat ttacgctgat tgaatgtcta
    16321 attggactga tgatattgag cagtctattg ctcacattca atcttttgat tcagcaaacc
    16381 atccaagtaa gtgaattttt gcagcgtaaa gatcaaaaag agtggctaat atttttagca
    16441 caattagaag aagaactcaa gagcagtcac aatgtctctg tgaaaaatca gcaactgcac
    16501 tacacgatcg aggataaaca gtatatcatc gaaagatatc aggcaatgat acgtaaaaga
    16561 agaacttccg gcggacatca acctatgtta acaagtatca ctgagcttca attattggaa
    16621 aaagaacaca caatttcttt ttatgtacac tttgaaaatg gagaagatgg atatgccata
    16681 tggacaaaaa acgatcagta gaaaatgtta taagggctct attttatttt ctgtcttgct
    16741 tttatttctt ttatgtacgt ttttattgat ggcagcatta gaaagctacc gtttgtcaat
    16801 ggatcttaat atacgaatca aaaattatta tatagcaaaa ataatgaagc agatggcttt
    16861 atcggaacag tctacgcttg ccgaaagttc ggaaggagtt ttttactaca caactggatc
    16921 tgtaacatat cagtgggtac aacagtccct attcttagag gtggaagtct caccgtttat
    16981 ctttcggttc acagaagaag taaaaaatga tacagatact agtacagaat gatggaatgt
    17041 tcgtgaaaac gccctctttt ctgttataac actgttttta ataaaaaaat gtttgccgtt
    17101 tcacaaataa aaaacagtga aaacgattga aattttgtca attgttcgat agaattaaac
    17161 aggtgaaaaa attgttataa tatatctaga aatgaggtgc accatgttac cagaaaaaat
    17221 ggaacaaggg tttaatcaaa acctggaagc tattcagctt ttacagaatg cattgggaac
    17281 ttccttttta gaagcctatg tcgaaaatgc agaaaattta atagatgatt atcaagtacg
    17341 tgtggtggat ggagtgccga caaaagagac aacgcaacgg atcacagctc tttatgaaga
    17401 gttgaagaaa ctttctttcg agccagaaga atggcgcaga ctgtctcaat tacttttatt
    17461 gaagggcagc cagacagaac atttgcaggc gaaccatcaa ctcactcctg atagtatcgg
    17521 ttttttattt gtttttctta ttgaacaact atacacaaat aaaaaagcac cggttaagat
    17581 tctggatatt gcggcaggaa tgggcaactt gttattaaca gttcttttaa acctatcgaa
    17641 tgcggggtac caaacagaag gaattggtgt ggatattgat gatacattgt tggctgtcgc
    17701 agcttctaca agtgatttga cacaagcaaa tgttcaatac tttcatcaag acgggttgca
    17761 ggaattatta atagatcctg tcgattttgc aatcagcgat ttgccaatcg gttattatcc
    17821 gaatgacgaa aaagcaaaag agtttttaac aagtaccgaa gaaggccata gttatgcaca
    17881 tcatttattg ctagagcaat caatgaaata tgtgaagccg gatggttttg gtttgttttt
    17941 gatgccgagt ggatttttag aaacggatca aagtgaagaa atcaaaaaat ggtttaaaga
    18001 agaaggttat cttcaaggaa tgattcaatt gccagatgaa ttgttccgta ataaacaatc
    18061 gcaaaaaagt attttgatct tacagaaaaa aggaccacaa gcacaacaag taaaagaagt
    18121 cttgcttgtg aaacttgctt cattaaaaga accagagaag gtgacggaat ttttcaacga
    18181 gtttaaaaac tggaaatcat ctgctctgta atcattcaac gagaaggaga gtcatatgtc
    18241 taaaacaatt gcaatcaatg ctgggagttc aagtttgaaa tggcaattat atcaaatgcc
    18301 agaagaaacc gtagttgcga aagggatcgt cgaacgtatc ggtttacaag attctatttt
    18361 taccatcaag tacggcgata atcaaaaatt cgaacaaatc atggatatcg aaaaccacga
    18421 agtagcagta aaaatgctgt tagatcagtt gattgaactg caaattttaa gctcttatga
    18481 tgaaatcacc ggagtagggc accgtgttgt tcacggagga gaaacttatg gggattcagt
    18541 agtgatcgat gagaaagtaa tggaccgcat tgctgaatta gcagaatttg ctcctttgca
    18601 taatcctgca aacttaatgg ggatcaaagc gtttaaaaag attttacctg acattttgag
    18661 tgtggctgta ttcgatacat ctttccactc aacaatgcca aagcaaaatt atttatatag
    18721 cttaccggtg tcttattatg aagatttcaa agtaagaaaa tatggcttcc atggcacaag
    18781 tcatcgttat gtgtcggaac gtgcggcaaa attattgaac aaaccgatcg aagaactaaa
    18841 aatcatcact tgccacttag gaaatggtgt atcgatcaca gcagtcgatg gaggtaaatc
    18901 catggatact tctatgggat tcacaccatt agctggggtg acaatgggta cacgttcagg
    18961 tgatatcgat ccagctgttc tcccatattt aatggaaaaa ttagagattg atattgatga
    19021 aatgatcaat gttttgaaca agaaatctgg tctgctaggc ctaacaggat tgtctagtga
    19081 tatgcgtgat cttgaaaaga actatgaaaa agaacatatt cgtttagctt atgacatttt
    19141 cgtggaccgc atccgaaaat acatcggcgg ttatgtgaca acgatgaatg gtgttgatgc
    19201 aattgtcttt actgctggta tcggcgagaa tgacggacat gtacgtagtg aaatcatcaa
    19261 aggtatgtct tggtttggtt gtgaaatcga tccagcgttg aatgaattgc gtggtgaaga
    19321 agtagatatc tctacaaaag aatcaaaagt aagagtatta gtgatcccaa cagatgagga
    19381 actaatgatt gcccgtgatg tggagcgtct gcgcggctaa gccaaagcat tcgttaggat
    19441 aaaagatgaa acgcatgcaa gagggaattc ttgcatgtgt tttttttgcg gagaagaccg
    19501 caaaatggta atttaacttc aatttccctt cttttttaca ggatcttctt agggaatttt
    19561 gcttggaaaa gtggtcaaaa gggaaaagac tggcatttta tttattgatt ttcaggaaaa
    19621 tcccaatatt ttattttcat tcatctttat aagggacatt acatatgtaa aaagaaaaaa
    19681 catttttaga tcagcctcgt gggctgtcga ctttattctt cgctttatgt ttagctagaa
    19741 aaccgatccg aaacattcat tgatcgaata ataaaaactt gattttccac ctgtactttg
    19801 cctgaatcgt gaaagcacag gtatgaaaat caagtttttt gttgcgaaaa aacgtcctag
    19861 tcaatcagtt ggccgagcgg ctggcggctg atctgctcct gatcaataac gagtgcttct
    19921 tggaagtgtt gctgcaaaaa gtagcggtca tggctgactg ctaggagcgt tccttgatag
    19981 tcttgcagca tttcttcaat ttcttctcga gcatagctgt cgaggtgatt ggttggttca
    20041 tccaaaatca ggacgttgat tttttctaaa aagagtttca gcaacgccaa acggattttt
    20101 tcaccaccgg ataaatcttt gatccgttta cccacatcgc tttgataaaa gccacttttg
    20161 gctaattgcc gtcgagcgac ttcttcgttt ccagtcaatg tgatggcgta agccagcaaa
    20221 cggctatcgg gatcggcaaa ctgcagctgc tgcggcaaat aaccgatttg caagctacta
    20281 cctaatttga tggtgccacc gtaaggcttt tcctcaccga gtaataattt gagtaaggtt
    20341 gattttcctg caccattgtc gccgatgatg gctaaatgat ctccacgata gactgcaaaa
    20401 ctgctgtctt tgaatagtac ttgatcagcg tactgctggt gcaagttttg aatgatgaaa
    20461 acttctttgc cagattttcc accattagac aagctttgca gccgattttt cgggggaggt
    20521 ggcggtttga cgagtgtcag tttcgccagg cggcgttcca gttctttggc ttttttgaag
    20581 aaggactcat tgtctccttc atgtgcccac tgacgaaaac gacgaatcat gactttcaac
    20641 cgctggactt ctttttgctg cagttcgtaa ttttttgtca gctcagccaa gcgagcttct
    20701 ttcaagactc gaaaacggct gtagtttccg ggatactcaa tcaatgcacc ttcttccagt
    20761 tcccatgttt ttgtggtgac ttgatcgaga aattgccgat catgagaaac gaccagaacc
    20821 gatttttttg tattctgtaa atagttttcc aaccagcgaa tactgttaag atctaaatga
    20881 ttggtcggtt catcgagtaa aagtaaatcg gcatcggctg ctaaaatttt ggctaaagcg
    20941 actttaactc gttcgcctcc gctaagttct gttaaccgtg cttcctgacg gtgtggaatc
    21001 cctaagcctt gcaagatcgc tgcaatccgg tcttctaata cgtaaccgcc tagttcttca
    21061 aagcgatgct gcagctcacc gtaacgggcc agacacttct ctaagtttgt atctggttct
    21121 gtcatcaatg cttcgtagcg actcatttgc tgtttgaggt cctgtatctc agcggcagaa
    21181 tggagcaagt agtggttgac ggtcgcttcg gaaactgtaa gttcttgttc gaccgtcccg
    21241 atctttaagc cttttttgcg actgataaca ccgctgtcga caccttctct gccggtcaag
    21301 atttgtaaaa aggttgattt gccagttccg ttgataccga ctaaggcaat tttttcacca
    21361 gcttcaattt gacaggataa atcctcaaat aatggattcc cgccgattgt ttttttaata
    21421 gaaattgctt gtatgatcat gatctctctc cttgagacca ctcaaaaagg ccatggttat
    21481 ccctggcctg tgttatttaa actcagatta tcatgagaaa aataccttga gatggccatt
    21541 gattttacgt gaattcaact gttttggaca aacttgtccc gtcaacagca actgtaaaac
    21601 caattgcatg gctaaatgcg atatggtatc gttggtaatt ttcattactt gttattttag
    21661 tcatgcgcat catagtattt tccctccttc gtgtagttgt cttttccagt gtacctgaaa
    21721 gtgtcagaaa gttcaactag cgctgacatt tacttaatat ttatcgatgt gtaattctgt
    21781 cagagagctt tttttatctg actgagggta atgtctcgtg gaccacatca ttgtattctg
    21841 agctaaacca gcgttcaggc gaaatcatat ggtgtttgat aaaaaacttt gtctacatga
    21901 cttccttttg ccatttgctt aataccgttg cattaaacag ctcttctctt tggaaatctc
    21961 accaacatcg cgttcaggag tatcgttgtg gttgcaatgg caagcggtag cctttcatca
    22021 attcttgaat gatttaccga atgtagcact tgccttctcg cggtacccga acccgaaata
    22081 aaattttagt ttggcgataa aatcggtatc cgccatcgat acttcatatt ttttcgtatc
    22141 agattcatcg gaaacttcca cattgacgaa ccaataagct ttggcatgct taggtcgttt
    22201 taaaccacac cagggcgact aatcgccctg gtgttcactt tttttatttt acttgaactc
    22261 attgactgtt ttccctttat tttaaacatt ataaagaccg atgagtgctt gccgcgtctt
    22321 ttgtttttat cacggttttt tgtgcaaaaa aacctgccga tagttggcag gtcgtctacg
    22381 tgttaagctt tggtgtggtc tttagctggc aattggtttt ctaaatcagt tcggacaaca
    22441 agcacatcgc aagctgcgtt tctaataaca tattcagaaa cagaaccaat aaataaacgt
    22501 tctacagcat tcaatcctgt ggctcctaac ataatcaaat ctacttgatt atcttctggg
    22561 atttctctgg caataatttg ttttggtgag ccatattcta cgacagaggt tacattattc
    22621 aagccagctt tcttcgcatt gctttcataa tctgccaatg tttgctttgc catttcagta
    22681 gcttgttctg caagcattcc atcaaaggat gaaacggttt ggaaagaacg tgtgtcaatg
    22741 acatgtgcta atagaagttc tccgttattt cggatagcta cattgaccgc ctttttaaaa
    22801 gctaattcag cttcatcaga accatctacg gcaaccatga tttttttgta ttgttgtaac
    22861 ataagcattc actcctcaag tcaatcgtat tgtactcttt tattgtagaa cactttaggg
    22921 aaaaagagaa ttatttactg ctttggttca gcaatttttt ttgcaaatcg gatactgaag
    22981 gtggcagaaa caataattac gatcagtaaa aagaccaagg caataaagcg ttgattgaaa
    23041 aaagctgtta aaatacccat tacgcctaaa acagcatatg cagcaccgta aaattgtagg
    23101 aatccttgat ttttatcatt tttgttgatc aatacaaaaa agatatcctg tttattaaat
    23161 aaataatagc taacgaataa taatactaaa gtggcgataa tcattaaaat cctaatcatg
    23221 aacaaatcct ccaaaataag caacaaatag aaaaagcaat ttgccaagag caaattgctt
    23281 tattctttaa aagatggtaa tctccgttaa tgccgttgtt tgtcctgtag tattgatccc
    23341 gcgaattcaa gcaggtgggt cccatggtcg tagcttcgaa ctaccaaatg ataaggcatg
    23401 ttaccagcag tgacacaaga cgggtcccaa gatatcaata aaaatgttcg gtcaacacac
    23461 aaagaggaca attcgaacat cacagatttc ccacctctat agtagcatat gaatttaaaa
    23521 agagcaatga gagagctcgc agaaacagaa ccgttttctt gtctagtttt ttggaggatg
    23581 ctttttccac tcttttatac ggtaatactg ttgacccaga gcctgttcat atttccctgt
    23641 atcttttggc tgataatact gagcattctt cagtttatct ggtaaatatt gctgattgac
    23701 ccaagcctga tcaaaatgat gaggatactg ataacccacg ccacggttga gtgatttcgc
    23761 acctttgtaa tggctatctc tcaaatgatc aggaacatct ccagctttgc cctctcgaat
    23821 atcagcaagt gctgcatcta atgccatgta ggcagaattg gattttggcg acaagcaaag
    23881 atcgacgacg acgtctgcta gtgggatacg tgcctcaggc aatcccaatt tttcagcagc
    23941 taatacagcg ttcactgtac gagccgcagc agcaggatta cctaaaccta tatcttcata
    24001 accgataacc atcagacgcc ggcaaatact tgccagatca cctgcctcaa ctaaacgggc
    24061 gagataatga agtgcagcat ccacatcact tccacgaatc gatttttgaa aagccgaaat
    24121 aacgtcataa tgagcatctc catttttgtc atgggtaaga gcttttcttt ggacacattc
    24181 ttcaatgatc gacagtgtaa gatgaatcct tccttcttta tctgaaggag tcgatagtgt
    24241 agccagttcc agcccattca atgcactccg caaatcgccg ttcgttgcac gcgaaaggtg
    24301 aagaagagca tcttcgtcta actggattgc ttgttgtcca aggccgcgtt ctttatccct
    24361 taaagcatgt tcgactgcca actggatgtc ttgttctgtt aatggtttga cttcaaagat
    24421 ctgtgtacgg ctgcggatcg ctggattgat ggtaatataa ggattctcag tagtcgctcc
    24481 gatcaagatg attcggccac tttctaaatg aggaagcaga aaatcctgtt ttgttttatc
    24541 taatcggtgt acttcatcca ataagaggat cactgtacca ctcatttttg cttcttccgc
    24601 tacaacttgt agatcctttt ttgaatctgt agcggcgttt agcatgcgaa atgcataatt
    24661 agtagagccg gcgatcgcac ttgcgatact cgtttttcca gtaccaggag gtccatatag
    24721 aatcatggaa gagagcatgc gagcatcgac catgcggcgt atgatttttc cttccccgac
    24781 tagatgctgc tgaccaacga cttcatcgat cgttcgaggt ctcatgcgat aagccaatgg
    24841 ctgttgcata ggattttcct ccttcttgtt tttacgtccc ttaattatat catatttagc
    24901 aagatgccta gaaagaacag ccttctgttg ttttgttttc atagcttatc caactaatat
    24961 tgtgtatgat aaagtaggtg agaaaaatga cggataaaca aaagatagat gaattttttt
    25021 ggaataaaac gacgccagaa gtggcacggg atttattggg gatgtatttg gagcatgaca
    25081 caccagaagg aagattagct ggatatattg tagacgcgga agcataccta ggtccagaag
    25141 acgaggccgc ccatagctat ggtttaagaa gaacaccgcg tgtgcgggca atgtatgaaa
    25201 aaccaggtac gatctacctt tacacgatgc atactcatcg gattttgaac atcattactc
    25261 agccagaagg gattcctcaa ggagtaatga tacgtgcaat cgaacccgcc gcaatgattg
    25321 atcaaatgag taaaaatcgt ggcggaaaaa ctggtccaga tatcagcaac ggtccgggaa
    25381 aattagtgga agcattagca attcctcaag aactttatgg tcagtctata gcagatagtt
    25441 cgttacgctt agtttttgag aaaaaaaaga caccaaaaaa gatcaatgct ttgcctagaa
    25501 tcggtatccc aaacaaaggt gtatggacag aaaagccgtt gcgttttgtt gtgtcgggaa
    25561 atccttatat ctctttacaa agaaaaaacc aaatcgagaa agattggggt tggaggaaag
    25621 aaaatgaaaa agaaggatct attaacatat ttagatgaaa taattgaaaa acaagtgaca
    25681 gactatgatg tagctttaga ttgggataca aaaaatcata cgatcgagat cgttgttcgc
    25741 ttgtttgcag aaaataaagc acaatttgag attgatgatg cagaagggat cgtttcgcaa
    25801 gaaccaatta tcgaatttga agacggcgta cttcttttta atcctcagaa atctgttttt
    25861 gatgaacgca attatctagc agtaatccct tatgaaggaa aaaaagggtt ggcaaaatca
    25921 gtagctgatt ctttagtgga atatctaaat gaagtattag ctcaagggca aagcgatttg
    25981 cttgattttt tggatgaaga cgatgacgaa gctgtattcg agcttcactg ggataatcaa
    26041 caactagcag aattagtaga agtaaaacag caaaatgaag cagatactta tttaccgtac
    26101 ccaagttatt aaggagtgac agatatgaaa tggaatgaag taaaagtaga aactgaaagt
    26161 gaagcagtcg aagcagttgc taatattttg atggaggcag gggcgagtgg tgtcgcgatt
    26221 gaagattctt tagatgttga acattttcag tcggatcaat atggtgagat tttagataaa
    26281 aatgatttta catcgctgaa agaaggcgca ctagtcatgg cttattttcc ggaaacgact
    26341 ttcttaccgg aaatcttgcc ttttatcaaa gatcgtatca cacaattacc tgagtttggt
    26401 ctagctattg gaaaaaataa agtaacagtt agtgaagtag aggaaagcaa ctgggcaaca
    26461 gcctggaaga aatactatca tccggtgcgt attacgcgaa tgctgacggt tgcgccaagc
    26521 tgggaaaact accagacaga agatcctcta gaaaaaatta tcacactaga tcctggtatg
    26581 gcatttggca caggcactca tcccacgact tccttgacat tgacagcact agaaacagtc
    26641 ttgcgtggtg gtgaaacatt gctagatgtg ggaactgggt cgggcgtgct aagtattgca
    26701 gcaaaatatc ttggtgcaaa agatgtctat gcctttgatt tagacgaagt agccgttcgt
    26761 tcggcgaaag aaaatatgga tatgaatgaa atagccaaag atgtccatgt atcagctaac
    26821 gatcttttga caggaatcga gattgaaagt gatgtaatcg ttgcgaacat tttagcagat
    26881 atcatcttat tgatgatccc agatgcttgg cgtttactga agcaaacagg cacgctgatc
    26941 gtttcaggaa tcattgaaga gaagaagcaa atggttctgg atgccatgtt agaacaaggg
    27001 tttatcgtgg atcaaatttt ccagcaaaaa gactggtatg caattatttt gaaaaaacca
    27061 gaggtggaat agatgcagcg gtatttttta aaagaatcat atgaatcaaa agactatttt
    27121 gttttagaag gggaaaacta tcaccacatt gtacgggtta tgaggatgga acccgaggat
    27181 gaagtttttt tggcattcaa tgatcaaata gccattgttg cagaaatcac ggagattact
    27241 gaagaggctg tttatctgaa agaaattaaa aaagaagaaa atcaaaagga actgccaagt
    27301 tcgattacca tagcgagtgg ctatcctaaa ggggaaaagc tggattggat cgttcagaaa
    27361 tccactgaat taggagcgca agcttttctt ggtttcccgg catcagcctc tgtcgttaaa
    27421 tgggatgcaa aaaaacgtat gaaacgacaa caacggcttg aaaagattgc tcaagaggca
    27481 gcggaacaat cccatcggca gatcgtgcca tcagtagagt tattggaaac gaaacaagat
    27541 ttatttgagc ggctagatga ttatgacgtg gtcttggttg cttatgagga atcagcaaag
    27601 caaggcgaaa aatcaaatgc agccaaaatt cttagtgaaa caaaaccaga tagtcgaatc
    27661 ttagctgtct ttggaccaga aggtggatta tcccgcgatg aagtcgatta ttttgtagaa
    27721 cacaaaggga tactttgtgg attaggacca agaatccttc gtacagaaac agcacctctt
    27781 tatttattaa gtgctgctag ctatcaatgg gaattaatga atccttcttt cagctaacgt
    27841 tcttcattaa taaaagagta tcgacagaaa gaattgaaaa aatagtcaac aatttgtata
    27901 atagaaacaa ttatttatac aatggatacg aaaaatcgtt tgaaaaagct tttaaaaagc
    27961 gaacatttcc gtaaaaaaac gtataactgc acggattttg ggacaattat caaacagata
    28021 tttgtcccga ttcccgtttt accgaaaaaa gaaaggggga tcgattcctg tgacaaaaga
    28081 agaaatttta acgggacccg gtgtcataaa gctcgttagt caatacatgg gtcccgaaca
    28141 tgtagcattt gttgagaaag catgcgaata cgcgactgct gcacatgacg gacagtttag
    28201 aaaatcaggc gaaccctaca ttattcatcc tatccaagtc gcaggtatat tagcagattt
    28261 aaaaatggat ccccatacag tggctacagg cttcttacat gatgttgttg aagatacaga
    28321 aatcacttta gaagatctga gagaagaatt tggcgatgac gttgctatgt tagtggacgg
    28381 cgtaaccaaa ttagggaaaa taaaatataa atcccacgaa gagcagctag cagaaaacca
    28441 ccgaaagatg ctgctagcaa tggctcaaga tttacgagtt atcatggtca aattagctga
    28501 tagactacat aacatgcgta cgttgaagca cctgcgagaa gataagcaaa gaaggatcgc
    28561 tcaggaaact ttagaaatct atgcgcctct tgcacatcgt ctagggatca gccggatcaa
    28621 atgggaatta gaagatacag cacttcgtta tctaaatcca aaacagtatt accgtatcgt
    28681 ccacttgatg cagacgaaga gagaagaacg tgaaaaatac gtaagtggta ctgttgaaga
    28741 tattcgaata gcgacagaag agctggggat ttttgcagaa atctatggac ggccaaaaca
    28801 catttattca atctatcgta aaatgaaaga tcagaaaaag cagttcaacg aaatttatga
    28861 cttgctagct attcgagtga tcgtagattc aatcaaggat tgttatgctg tattaggagc
    28921 aatccacaca aaatggaaac caatgcccgg cagatttaag gattatattg ccatgccaaa
    28981 agctaatatg taccaatctt tgcatactac cgtgatcggt ccggcaggaa atccggtaga
    29041 aattcaaatc cgaacacagg aaatgcatga aatcgctgaa ttcggggttg ctgcacactg
    29101 ggcctataaa gaaggaaaaa atgaaaaagt agaaccagat ggtatgacga aacaattaag
    29161 ctggttccat gagatactcg aacttcaaga cgaaagctat gatgcttctg aatttatgga
    29221 aggcgtaaaa ggagatatct ttagtgataa agtctacgtc ttcacaccaa aaggagacgt
    29281 tactgagtta ccaaaaggat ccggaccatt ggactttgca tacagtatcc atacagatat
    29341 cggtaacaaa accactggtg caaaagtaaa tggcaaaatg gtgcagcttg attacaaatt
    29401 gaaaaacgga gatatcattg agatcatgac ttctccaaat tcatttggcc caagtcgcga
    29461 ctggttgaaa ttagttgcta ctagcaaagc aagaaataag atcaaacgtt tcttcaaagc
    29521 ccaagatcga gaagaaaatg tgatcaaagg ccacgaatcc gtggtcaaat gtattacaga
    29581 tctaggattt acgcctaaag atattttgac gaagaacaaa ctgcaagaag cactcgatcg
    29641 ttttaattat caaacagaag atgatctcta tgcagctgta gggtatggag aagttagccc
    29701 cttgacgatg gccaatcgtc tgactgaaaa agaacgtaaa gaacaaaaaa tcgagcagca
    29761 aaagcaagaa gcagaagaaa tcatgaatca gccgaaaaaa gaacctgaca aaatgaaagt
    29821 acgtcatgaa ggtggcgttg tcattcaagg ggtagaaaac ttactaattc gtatcagtcg
    29881 ctgctgtaat ccgattcctg gtgatgatat cgttggttat atcactaaag gcagagggat
    29941 atccattcat cgtcgagatt gtccgaatgt tcagcctgac aaaccaaatg tagcagaacg
    30001 tttgattgaa gtcgaatggg aagatacatc gaatacacga aaagagtatg atgcagattt
    30061 ggaaatttac ggctataatc gttcaggctt attgaatgat gtacttcaaa cagtcaatgc
    30121 gctaacgaaa aatctcaaca gcgttgaagc acggacgaat aaagataaaa tggcgacgat
    30181 ccatttgacg gttggtatcc agaatttatc ccacctaaag agtatcgtgg ataaaatcaa
    30241 agcagtacct gatgtctaca gtgtacgccg gacgaatgga taggaggaca gatatgagag
    30301 cagttgttca acgggtaagt aaagctgctg tgtcagtcga tcagcaagaa attggaaaaa
    30361 ttggctacgg cctacttgtt ttattaggca ttcatgaaac cgatacgcaa aaagatgtgg
    30421 actatctggt aaaaaaaatt gcacaaatgc gtatttttga agatgaacaa ggaaaaatga
    30481 atattagtgt agaagatgtg ggaggacaaa ttttaagtat ctctcaattc acactgtttg
    30541 cggatacgaa aaaaggaaat cgaccaagct ttgtggaagc cgcacgacca gaaacagcta
    30601 ttcctttata cgaatcattc aatgaaggaa tcagaagtcg cggcatcaca gtcgctaccg
    30661 gacaattcgg agcagatatg tcggtttctc tcgtcaatga tggacctgta acgatcatta
    30721 ttgattctaa aaacaaataa agtaatctgg ttttcactag taaaaactat gaaacacgtc
    30781 gcttaagtta gactgataaa ctctaactta agtggctttt ttgtctgcaa aaagcgcttc
    30841 ttttgtcagc agtcggaaaa aaagttaagc ttaaattata caagttattt tttataggag
    30901 gttgctgaag atgaaagaag taccagtagt agatatcaaa atcacagatt taaaaaaatt
    30961 gtatcaaaaa ggatacaaca tgttggagga gttacgccat gaagcggacg ctcccgtcgt
    31021 caaagcaaag attttcaata aagaagctat cacgatctac ggatcatcag cagcaaaagt
    31081 attttatgat ccaaggaatt tcaaacgaaa aggtgctatg ccaaaattag tattgaagac
    31141 actttttggt caaggtggag tacagacttt ggatggagct gctcatcacc atcggaaaaa
    31201 tatttttatg gatctaatga caccggaaag aatggaagat tatcatcgta tcttagataa
    31261 gaatctgaca caagcacttg aagcacagca tggtcaattt gaactatttg atttaagtaa
    31321 gatggttttc tttacatcta tttgcgagtg ggcaggtatc aatttatcag cgatttctaa
    31381 agatgaggtc gaaaaattag cagaatatca aatctctatg atcagcggaa cgtttacttc
    31441 tccgattgat cacataaaag gtgtcgagaa tcgcaaaaaa tcagaaaaat gggcgcaagg
    31501 cttgattgaa gaagctcgac aaaatcctgt ggctggaaaa gaaaatgtgg cattatatgc
    31561 ttttgccaat gccacagatt tagatggtca gcttttacct ctggaagtgg cagctgttga
    31621 attattgaac atcatccgtc caacggttgc tctaactgtg tgggcggcat tgatgggaca
    31681 tgcacttttc agtagaccag atttgtatca gcaattaaaa aatgactttt cgacgttaca
    31741 agatccgttt attcaagaaa tgagacgcta ttatcctttc ttcccaatgc tacctgcgat
    31801 ttcgctgaaa gaagtagaag tagatggtta tcgtatacca gaaggcagtt gggttatttt
    31861 agatttatat ggaacagacc atgatgaacg aacagttgaa gcgccagatt cctttatgat
    31921 caaacggtat gtagggaaag caaaagatat ttcctataaa gaagaatatg agatgattgc
    31981 acaaggcggg ggaaattttc gacaaatgca tcgctgtgca ggagaatgga tcactcttca
    32041 tagtctacgt gtgttttccg atcagctagt aaataagttt gagttctctg tacctgaaca
    32101 agattggacg attccattca atcagtttcc gacttatccg aatagccgag cgctacttta
    32161 caagaattag attagcataa acttatcgaa aaaaaggaag atcaaacagt gctgatcttc
    32221 cttttttcat attactataa attgataagg tcaattgatc aaaatccacg aaatgcttca
    32281 tcaatggttt gataatcttc attggataaa tgcaaatcta aagcttggac attgtcagat
    32341 acttgctctg gacgcttagc tccaggtata actacagaca ctcgtggatt cttcatgtac
    32401 caagctaaaa caagttgcgc aggagtggct tggtatcttt tagcaatggg gcgaagtaca
    32461 tcgacagcgg ttaaaatttc tctgaaacgt tctccttgga aatctggatt tctttttcga
    32521 ggatctcctt caccaaattg tttttcctca ccaagttcat attttcctgt cagcaaaccg
    32581 gaagcaagag gaaaataggg aacaaaactg attttgtttt tttcaagata agggaataat
    32641 tctttttcag cctgccgatg gatcaaactg tatttatctt ctactacatc gacatatcca
    32701 tctgcattcg cttctttcaa ttgttctaac gtgaagtttg acacaccgac tgctcggatt
    32761 tttcctgctt cttttaattc gtgcaatgta gcaactgatt cattttttgg tgtgctttca
    32821 tccggaaagt ggatataaaa aatgtcgatg taatcagttt gcagacgttt tagtgcatct
    32881 tccaccgatt gtttgagaaa ctcaggagaa ttatcaaatg ttcgcccttt atttggtaca
    32941 tgagcagctt ttgtagcgat gacgacacgt gagcgatcat attctttttc cttcagtacc
    33001 tcgccaatca gttcttcgga acggccgttt ccgtaagcat aagcggtatc gatcaaattg
    33061 atcccgctat tcaaagctgt acgaactact tgttttcctg tctcatcttc caaaccgtca
    33121 aaaagattat gtcctccaac ggcatttgct cctaatccaa gctgttctgc gtagacttgt
    33181 gaatgtccga ttcttatttc tgccatagct gattcctcct ttttgtctag tctaaaacaa
    33241 aataccaaaa agctgaacta aaacgttttt gtgaggggaa atagtcagat atctcttgac
    33301 tttgagactg gtttttagta ggttataagt aatatcgaat gtcaatgaaa gagcgagtaa
    33361 gtttttgaaa ctttacaaga gaggatcatc gaggctgaaa atgatctaaa gtttcattag
    33421 cctaaagaca ctctggagat ggacgagtga aaagccgtcc cgcgtacgag cgttattcgt
    33481 aagaggaaag agaaatcttt caaaattagg tggtaccgtg tatgaatttc attcaccctt
    33541 gttcatgatg aacaagggtg atttttttat ttacatagct aagaaaggga gagaaaacta
    33601 tgagtttcca aagaccaaaa ggaacaaatg atattttgcc aggcgaatca gaaaaatggc
    33661 agtttgtcga agaaacagca cgtttattat tcagagatta tcagtataat gaaatgcgta
    33721 caccgatttt tgaacactat gaagtgatat cccgaagtgt aggggataca acggatatcg
    33781 tttcaaaaga gatgtatgat ttttatgaca aaggggaacg ccatgtgacg cttcgaccag
    33841 aaggtacagc accaatcgtt cgttcttttg ttgagcataa attgttcggc ccagagtatg
    33901 cgaaaccgtt caaaactttt tatatgggac caatgtttcg ctacgaacgc cctcaaaaag
    33961 gacggttacg tcaattccat caaatcggag tagaagcatt tggatcagaa aaccctgcaa
    34021 ctgatgtgga gagcatggtg atggcacttg atttcttcaa acagttgggg atcacgcaaa
    34081 tccggctagt catcaattca ttaggtgata aagaaacgcg tcaagcctat cgtcaagcat
    34141 tgatcgacta tcttttacct tttgaatcag agctgagtga agactctaaa cgccgccttc
    34201 atgaaaatcc attacgcgta ttggacagta aagacaaacg agatcaaaaa tttgtggcag
    34261 aagctccatc tatcttagat tatctaagcg agcctgctca aaaacatttt gataccgttg
    34321 tttccatgct ggatgcattg gctgttcctt atgaaatcga cagtaatatg gttcgcggac
    34381 tagattatta cacacatacg atttttgaaa tcatgagtga cgcaccaaaa atgggggcac
    34441 aagcaacgat ttgcgcaggc ggacgttatg acaatctagt ggaagaacta ggtggtcctt
    34501 caacacctgg cttcggattt gcaatgggta tcgagcgatt actcttgact atggaggcag
    34561 aggaagtagt tatccctgca tttaatgagt tggatgctta cgtggtcgca ctcggagacg
    34621 aaacaaatat cgaagcattg aaagtggtcc aagcgattcg aaactttggt ttttcagctg
    34681 accgcgactt catgaaccgt aaagcaaaag cgcaatttaa gacagcagat aaagcgaatg
    34741 ccaaattagt attggtgatc ggtgaagatg agcttgctag tcaaacgatt aatgtcaaag
    34801 caatggcaag ccgaaaagaa aaaagttttc cattagaaga gatatatgaa cattttgatg
    34861 aagtctatga tgaaatgact ttattcgagt aggaggaaaa aaataaatgg cacaaagaac
    34921 gatctattgc ggattagttt ccgaacaaca attagaacaa acagttacat taaaaggatg
    34981 ggtacaaaaa agacgagacc ttgggggagt aatctttatc gacctacgtg atcgtgaagg
    35041 aatcgttcaa atcgtcttta atcctgaaat caacaaagaa gcatgggaaa tcgcggataa
    35101 atgtcgcagt gaatacgtga tcgaagtgac tggggaagtt agaagacgcg gagaaaatgc
    35161 agtaaatcct aaaatggcca ctggtgaatt cgaagtgatt gcaagtgaaa tcgtgatttt
    35221 gaatcaagca aaaaccccgc catttcaaat tgaagatgat caagcaatca gtgacgaaat
    35281 acgtttgaaa tatcgttatt tggatctgcg tcgtccaaaa atgactaaaa acatcaaatt
    35341 aagaaatgcg acaacaaaag cgattcgtca ttatttagat gataatgact ttctcgatat
    35401 cgaaacacct tacctaggga aatctacacc agaaggcgca agagactacc tagttccttc
    35461 acgtgtccat gctggacatt tctatgcgtt gccgcaatct cctcaattgt ttaaacaatt
    35521 gctgatgaat gctggattcg accgttatta tcaaatcgtt cgttgtttcc gcgatgaaga
    35581 tttacgtgga gaccgtcagc ctgaatttac gcaagtagat attgaaacaa cgttcttaac
    35641 agcagaagaa atccaaactt acacagaggg attgattgcg aaggtaatga aagaagttcg
    35701 cggtatcgaa gtcacattgc catttcctcg tatgacctat gatgaagcaa tggctcgcta
    35761 cggcagcgac aaaccggata cacgttttga tatggaattg atcgatctaa gcgatacagt
    35821 caaagaagta gaattcaaag tcttccaaat ggctcttgaa aacggcggtg tcgtgaaagc
    35881 attgaatgct aaaggcgctg cagatcgcta ctcacgaaaa gacatggatc agttaggtca
    35941 atatgtagga caattcggag ctaaggggct tgcatggctg aaagtagaag aagacggatt
    36001 gaaaggtcca attgctaaat tcatgggaga agcaacagaa gcaatcatta aagcaacaga
    36061 tgcaaaacca ggtgacttgc tgatgtttgg cgcagataaa agtgaaatcg tcgctgctgc
    36121 attaggtgct atccggacac gtttaggaaa agaattagga ctaattgatg aatcgaaatt
    36181 caatttctta tgggtaactg attggccaca atttgaattt tcagaagaag aaggtcgata
    36241 tgtctctgct caccatccat tcacgatgcc aaaagaagaa gatatcccgt tattggctga
    36301 agaccctgca aaagtctatg cagaagctta tgatatcgtc ttgaacggtt atgaattagg
    36361 cggaggttct atccgtattc atactcgtga gcttcaagaa aaagtactag aaactctagg
    36421 ctttacaaaa gaacaaatgg aagaacaatt tggtttcttg ttaacagctt tagattacgg
    36481 attccctcca catggcggta ttgcattagg attggaccgt tttgcaatgt tattagccgg
    36541 agaagataat attcgtgaag tcattgcctt tcctaaaaac ggaaaagcag cagatgtgat
    36601 gagtgatgca ccaagtatgg tttcgccttt acaattgttc gaattgaata ttgatgtaac
    36661 gaatatagaa gaatagccga taaaaaggta aaaaggattt gtcgaatacg cttcttttga
    36721 caaccttgat ttaattgaaa aacactttat aatgaagcca taacatataa agcggggctt
    36781 ttccatgaaa aaaacattcg aaaattgggc ttatcatgat tacacgacca agctttcggt
    36841 atctatcgtt tatgcgttga tcgcatcagt cgcattgaat ttcttttatc agccaggaca
    36901 tatttattca agtggtatta ctggtctagc tcagattttg acgactttgt caaaatcact
    36961 gataggattc gaagtaccgg tatcaacaac tttgtattta ctgaatatac ctttgtttgt
    37021 attggcgtgg ttaaaaatcg ggaagaagtt tacaattttc acttttatca cagtcacttt
    37081 gacctccctt tgtatccata ttttaccgga aacagtccta tctaccgatc caatcatctg
    37141 tgcgttgttt gggggggctg tgatgggact aggtattggg ctagcgttga gaaacggcct
    37201 atcatcgggc ggtctagata tcgtgagtat cacgattcgt aaaaaaacag gaaagtcgat
    37261 tggttctatt tcgatctact tcaatgcatt gatcgttttt gttgccggaa ttctatttgg
    37321 ctggcaatat atgttttaca gtacgttgtc tattttcgtc agtggaaaag taactgatgt
    37381 cgtttatact aaacaaaaga agatgcaggt aatgatcgta acgaaacatc cagaaaaagt
    37441 agtaaatgaa atccaaaact gtatgcgtag agggatcacg atcatcaatg aagcagaagg
    37501 tgcttataag catgataagc agacagtatt gatgacgatc gttacacgat acgaactgcc
    37561 tctacttaga gaagcaatga aagcttctga tccacaagct tttgtaagta ttgcagaaaa
    37621 cgtccagata cttggtcgtt tctatgagga tggcatttag atcgagtttg acaaaaattc
    37681 atgaaatact gctgtagaca gacgttcgtt tgtctgcagt ttttttctac catagtagaa
    37741 tcagcaaaga aaaactgctt ttttatttga aattttctta aagttgaaaa aaagcatgtt
    37801 tattttttta aatttatggg atgatagaaa aaaatagtag agaaaggaca cgattctact
    37861 tgctattttc acttaatacc cgtatattac gaatgaaaaa agtttatgaa aagttggaat
    37921 attgaatgga aaaacaaaaa aggttaaaaa acagccgcta catcactggg tttgatgggc
    37981 tccgttcatt agctgtggtg ggtgttatct tctatcacct tctgccaact agtatgaaag
    38041 gcggctattt gggcgttccg atttttttcg tcgtatctgg ttatttgatc acggatctgc
    38101 tgcggcagga atgggaacaa aatggaaaaa tcaatatttg gcaattttat gtcagaagaa
    38161 tgaagagact ctatccggca cttgttttta tgcttgtgac agcttcggct tatatcactt
    38221 tgtttcagcg agggctgtta aacaatcttc gaggagttgt ggtcagcagc ttactgtatg
    38281 tcaacaactg gtggcaaatc aataacggtt tatcttattt cgatcgattt gctaatgagt
    38341 caccgtttac gcatatctgg tctttagcag tagaaggtca aaactatttg gtttggccga
    38401 tcttgtttgt attgttgatg gtatttgttc gtaaaagaaa atggatcttt tatactgtgt
    38461 taggtacatc cttcctctcc gccatcttga tgatgatctt gtatacacca ggaggcgatc
    38521 caacccgtgt ttattacggt accgacactc gtctgttttc tatatggttg ggaagtgcgc
    38581 ttgcgtttgt ttggccaagt acgcgtttaa agaaaaacat cccgaaacaa gcgaaaaata
    38641 ttctgaatat cgctggagga atctcgcttt tggcactgat tctttgcttc ttctttttag
    38701 atgatcatta caatttcatc tattacggag gaatgctcct agtgagtctg ttatgcgtag
    38761 tgctagttgc agtgacagca caccccggag caagtttgaa tcgatggctg acaaatccag
    38821 tatttacttg gattggcaaa cgaagctatg gcatttatct gtatcagttt ccagttatga
    38881 ttttttatga agcaaaggtg actgatattg ccgatcatgt tttgttgcat actttagtgg
    38941 aaatcacatt gattctaatc atcagtgaac tttcttatcg atatgtggag cgtccgctag
    39001 cacgttttga ttatggtcgt acattccaag tgatgaaagg atggttcagc aaaccgatca
    39061 tcagtaagag caaaccatgg gttgttccag gcacattact tgtagcaatt gcactcgttg
    39121 gtttcgttac ggcaccgaaa aacgctgtaa cagccgatca aaaacaactg caggcaaaaa
    39181 tcgctgaaag taaaaaatta gcagaagaaa ccagaaacaa ttcagaaaac accactagtt
    39241 cggttgacca agcgatcctt gataagtacg agttgtccaa ggcacaaggg aataaggcac
    39301 aaaaattaga actaacggca tttggtgatt cagttatgtt ggatgctgca acggatctac
    39361 aagaacttta tccaaaagta gtggtagatg gagatgtcgg acgacaatta tatgccagtg
    39421 taccgtacat tgaaaaatta aaggaacaag atttgttgaa agacactgtt ctgattggac
    39481 taggaacgaa tggctcattc tcggaagctc agtttgatag tgtgatggat gcaatcggtg
    39541 atcgaaaagt atactgggtc aatgtccgag taccgacgaa gcgctggcaa aatgacgtga
    39601 atgcgatgct tgaacagatg acgaaaaaat acgacaatat gacgctgatt gattggtaca
    39661 gctacagtaa tgaccatagt gactggtttt atgatgatca agtccatcca aatcctgaag
    39721 ggatgacaca ttacattcac ttagtcagcg aagccttgct aggaggaagt tcaagtacga
    39781 acagcagtga gacaaacgtg acagaatgat agctacaaaa aacttcaggc aaaaacattt
    39841 gtttttgcct gaagtttttt tatatttacc gtgtttttaa cattcatata gattcaggaa
    39901 gcaaaacggt tgagtgtgtc tgtaaataac gttctttttg ttttcgggtg agttttttga
    39961 atgcagcttc tgcttttgtt gcttcgcttc ttgaagaaaa tacttctgca tggatcatct
    40021 taacaggtct tcttttttga agacgagtat atttagcacc agtaccattg ttatgttcct
    40081 ctaatcgtct agaaagatcg gttgtatatc caccataaaa tgtctgatct ttacagaata
    40141 atacgtaaaa ataatgttca tttgccatag agcatctcac gaatctctgc agtgtattct
    40201 ccatccatat tgtaagtgat caatggtgga gcgactttga atccgtctaa tttgccttct
    40261 ttgattcctt caactaacaa aatatttgcc tcttttccag gttttgggta aacaaattgg
    40321 atccttttag gtgcaattcg atagtcttcc atgacctgaa tgatttctaa gaaccgatca
    40381 ggacgatgga ccatagtaaa gcgtccattt gtttttagta gcttacttga aatatcgatc
    40441 acttcttcta acgtcgtatg aatctcatga cgggcgattg ccaggtgttt attagggttc
    40501 gttttatttg ttggcagccc tttgaaataa ggtggattac agacgactaa gtcacaagaa
    40561 ttatgtcgaa cgacagataa gctgtctttc agatcgatgg tgtggacagt catttgttca
    40621 tttaaatggt taagcgcgat actacgtctt gccatgtctg ctagacgagg ttgaagttcg
    40681 atagcgtcga tagccgcctg ggttcgttta cttaaaaaaa gtccaactgc gccatttcct
    40741 gcacaaaggt caacaatctt ccctttttta ggtacacggg aaaagtgagc aagcagcaca
    40801 gcatctaaag aaaaagaaaa cactttggaa ctttggataa tcttgatatc ttctgcgtat
    40861 aattggtcga ttcgttcgtc ttcgtgaagc atggattccc tccaaaatga aaaaaatagt
    40921 cgtctgtatt atactatttt tattaaaaag aaccaaggtg gcttgtcaaa cctctatttt
    40981 ttatgcatac tatgaccata agaagggaaa ggaagagact atgttttatc gatttatgcg
    41041 cggggtagtt cgagtactat tagccttagt taatggaaat gcccgctatg agaataaaga
    41101 agttttaccg caagatgaga attacatact agttgctccc catcgtactt ggtgggaccc
    41161 attatatctg gcggtagctg cagcacccaa ggaattcagc tttatggcaa aagaagaact
    41221 gttcaaaaat ccagttctgc gatttatatt aactcgatcc aatgcttttc cagttaaaag
    41281 agaaaatcca ggaccaagcg tcattaaaac acctgtaaag attttgaaat cgacaaattt
    41341 gagtttgatc atgtttccca gtgggacaag acatgctaca gaacttaaag gcggaatggc
    41401 attgatttcc agaatgtcta aagcgaaaat cgtgccagct gtatatcaag gtccgttgac
    41461 acttggggat ctgttcaagc gtcagagagt gacagtgcgt ttcggcgaac caatcgactt
    41521 atcagatatc aagaaaacgg ataaagaagg tatggcggag atcgaacgac gaacacaagc
    41581 agcttttgac aaattggaca aagaagtgaa ccctgatttc aaatatgaag tcaaaaaaaa
    41641 ataaaatgat aagtttatgc gtgggcggtc ttttgaatct actgtcctgt tcagttcaga
    41701 ctttttcaat gaaaacgagg gagagacgtc aagtctgccc tctttttgcg ttccaatatg
    41761 taaaatagaa gacaggtgtc atgtaatgga tcaaagtgtt ataataatta gaaggacatt
    41821 ttttgaggtg attgaatgcg ttttttacat acagccgatt ggcatatcgg aaaaaaacta
    41881 caaggttatg acttgattga agaccagcgt catgtactat cttctatact agatatcgcg
    41941 aaagaagaaa aagtagaggc aatcgtgatt gccggagact tatatgatag gagtgtccca
    42001 gctgttgatg ccgtagaatt atacaatgaa ttgatggtcg attggaattt aaaggaaaag
    42061 atcccgattt ttgctatatc aggaaatcac gatagcagta cgagactttc agcgggtacg
    42121 ccatggttca gttattcgaa ctattttttg cgaacacagc tagcagaagc atttgaacca
    42181 gtcgagttta aaaacgtaca atttttcttt cttccttact ttgaaccaat tgcagcgaga
    42241 ttatattttg aagatgacaa tatccgaacg atcaagcaag cggtagccaa ggtgatagat
    42301 aaaatgcagg agctatttga ttcagaaaaa aaacaagtat tgattagcca tttttttgta
    42361 atgggaagtg taaaatctga ttcggaaacg aaaatcgaag taggcgggtt ggattcgatc
    42421 cctggtgaac tacttacacc gtttgattat gtagcactag gacatttgca taatcaagct
    42481 gctttgagac aggataatgc tcgctatagc ggttccccat tgaaattctc tttatctgag
    42541 atcaaccaaa aaaaaggcgt atggatcgtt gacctgacaa caggcctgga gttgaatttt
    42601 agggagatcg aacccctgca tgatattgaa gaaataacag cgagtttcaa agaattgctg
    42661 gatcctgcct tttatcagtc cgtcaatcgg gagaattaca tctatatcta tctcactgat
    42721 agaacgatca tcccgaatat gatgaaccag cttcggcaaa tatatcctcg tattttgggg
    42781 attgaacggg caaacggtcg agcagaacaa acaaagcgca aacgaaaagt actagaaatg
    42841 aaagatcctc aaaaattagc agaagcattt tttagtgaaa tgacggaaga gcagctaact
    42901 gcaaaccaag aaaaatggct cggagaaacg ttgcaagaaa tcatggaaat ggaatgatca
    42961 agaatgaaac cgaaaaaatt acaacttaaa aattttggtc cttttatcaa tgagaccgta
    43021 gatttttcac gtttgacaga agcaccgctt tttttgatca gcgggaagac aggagctggc
    43081 aaaacgacga tttttgatgg gatcaccttc gcactttttg gtgaaacttc cggaagactt
    43141 cgctctggaa aagagatgcg ttctttgttt gctacaccag acgaagaaac aagcgtgact
    43201 ttttcttttg aacatcagca gatgagctat gaaatcgaac gaaaaccaga acaagtttta
    43261 gcaaaacgaa aaggaaacgg aacaaaaaaa caggcagcca aagtaacgtt gaccattttt
    43321 gatgggaaag gaaaagaaat aaaacaaatc acaaaacgta cagaagcaga tcagttgatc
    43381 agagaactga tgaatctcga tgcgaaacag ttttctcaaa tcgttttact accccaagga
    43441 gaattccgta attttttggt ttcatcaagc agtgataaag agactgtttt acgtcattta
    43501 ttcggtactc aatttttcca acagttcaat gagcgattaa aagaaaaagc aaaacaacag
    43561 caacaaaaat tggatcattt agagcaagag ctgcatcttc tgcaaaaaag gtttgtttta
    43621 gtcaaagaag aggaaccagc agcagggttt gaagccgttt tagcccaatg gacaaaccat
    43681 caggcaatac tcgaaaaaca aatcacggaa gaaaaagaag ctctaacgga acttcaggaa
    43741 aaacaaaagc aactagaaaa ttcctactat cgttttgaaa gcttgaaaaa aagttatgaa
    43801 gaaaaaacga tattaataaa caagcaaaag gaattagctg ctcaaaaaga cgaaatagag
    43861 gaaaagaaac gatggattgg gtattatgaa tttacgcaac gattagtcga accaatccag
    43921 cgcctacaag aaataaaagt tgaaaaaata gaactgtcac aaaaaagtag tgagcaaaca
    43981 aagaaactag cgatgactac gcagaattat gaagagtggc aaaaaaatga agcaaaccgc
    44041 gtacgccgac aaacaaaaat caaagaagct gctcaaaaac tgcaaaacaa acagatgatg
    44101 gtgccaattg tcgaatcctt acgtagtaaa aaacaagacg cagaacaatt aacaatttca
    44161 ttggataaag aagaattgaa tgccgagaag ctgtatcaaa atctcgctat ctatcaggaa
    44221 agaactcgaa ttcttcaaga aaatttactg gatcaggaaa aactgcaaga agatcgtctt
    44281 gcttttggac agttaaaata tctagatgaa aagcagcaag aaatagaaaa acaaaaagca
    44341 aaagacactg ggtcgctcgc acagataaga gaagaactca aaaaagcaac tgaagaaata
    44401 acaaaaaata gtcagctatt gaaagaacag acagaacaag cagaacagct gaaaagccaa
    44461 tgggcaaagc agcaaattgc tcgtcttcag ctttttttgc gtccagggga gccatgtctt
    44521 gtttgtggat caacggaaca ccccatacaa caattaaaac acgaacagcc aaataaggaa
    44581 gatatcttaa aagtagaaca acaactggca gaagctgagt ctgctgtaga aagaactcag
    44641 caaatcattg ccaaaagtga agcacatgaa aataatcttc aacaacaatt atcagattta
    44701 gaacaaaaaa tcatggcaac gaatatacaa ttggctgaac aagaagaaca cttgcaacag
    44761 gctttgaaaa aggcatttcc tgagataaca gaacaaaaaa caaaaacaac tcttgtagta
    44821 atcgaacaat ctttagatag acgaaaaagc aagatggcca atgcaagaat agagctggaa
    44881 gaaataagac aacaagcaga aaagctcgaa caacaattag ctgtctacaa acagcagcag
    44941 acagaaaaaa ataatcagct ccagcaactg caaggagaaa tcaaaagttt agaacaacaa
    45001 ttgcaagata cggatagtaa ctgtttagaa gaggagatac gacgacttga aaaacagtta
    45061 gaagaagact cgaatcaact agctgttgag cagaaagcag gagtgcaatt gttgcaagat
    45121 cttcatacgc ttcggacaca gcttgatctt caacatgaac aaatagaaaa aatagatgaa
    45181 aaaatagaaa aggaacaaga aagaatagct aaaaagttag aaagccagtc gtatttttca
    45241 acaattgaag aaatctgtgc atttgcacaa cagcaagcac aatataaaga aaaccaacgt
    45301 acagttactt cttatcatga agagcaatta gtgacagctg accggctgcg tcaattagaa
    45361 aatatccagc taactgagac attcccagat acagaagaga tcagccaaca gttagaagaa
    45421 ataagacgta tgatcgaaat aaagcaagaa aagatatacg gtttacaaga acaaaaaatt
    45481 agtaatcaaa aaatctacga tgaatgtctt tcgatttatc acacaagcca gacgaaatta
    45541 gatgagctta cgcaattaca gcagctttcg caaacattca acggagaaaa tcctaagaag
    45601 actagtttgg aacggtatgt gcttcaggtt tatcttcaag aagtgttaca agtagcaaat
    45661 gatcatctcc aacgtttaac gaaaaatcgt tatcaatttg agctagcgga taccattggc
    45721 agttatcgag ggaaaaccgg attagaaatc aatatctatg atgatcaggc aggtatgtct
    45781 cgaagtgcgc ataccctgtc tggcggtgag agttttattg cagcactttc tttagcatta
    45841 gctcttgccg atgtgatcca gacacaagct ggtggtgtag caatcgatgc attgtttatt
    45901 gatgaaggat ttgggtcatt agatgaagaa gctttggaaa tggcaatgga agctttagaa
    45961 acgatcgaaa gtgaaggaag aatgatcggc atcattagtc atgtaagaga attgaaagaa
    46021 cgagtattac aacaaataag aattgataca gaaggtagcg gacaaagcaa aatacgctat
    46081 caacttggat aaaaagggag attggtaact atgaaatgga gtattccaga aaaagtcatt
    46141 gcacgtggac gtcaatactt gcaagaaggc cgagtgttat cggttgtccc tgaccaagaa
    46201 gaaaacgtgt ggcatgcaga agtcttgggc agtgaattgt atatggttga tttggatggt
    46261 acagcaaaag agatagatta ctgccaatgt ccctattggg aggaacataa atattgtaag
    46321 catacagttg ctgtggaact ttatttacgg tcgaaaaatc tatctcgtat catgaaaaaa
    46381 gaccagccaa tgctagaacc ggtaaaagca acaagtgagg gagaaatcat tgcaaaaggt
    46441 ttttctcgtc taaaaacaca aaacaatcct caagcggtgc aacctttgat tattgaatac
    46501 caagtagaaa cgattgagac taatcagtat cacccagagc tttctatttt ggcactttac
    46561 ttgcgtatcg gttcattggc atcaccgaaa aaaacatata tcgtaaaaaa catctatgat
    46621 tttttacaag cttacacaga aaaagaaact tacactgtta ataaacagta ccagtttcgt
    46681 ctagataaac gggcgtttca aaaagaagat caaaaaatac tcacgcatct tgcaggaagt
    46741 gctcagacgc atcaattatt aggtacgaac ggcttacaag tcaaaggaaa actagataaa
    46801 agatatctgc ttctcccagt agaacaagcc cgcttcctgt tagagcagat gaatcagact
    46861 acacgtttgc atctgcagat aaatgaacaa aacgatcaag gaatacaatt ttccgatcaa
    46921 aacaaacctt tatcattcaa agtggaaaaa tttggagaag attatttgct gcaagcattg
    46981 aatgattttg attttttctt tatgcattat cagtggggag ttatccaagg gacattttat
    47041 tctctgacga aatatcaaca aacggtttat caaacttgga aacaattgat tcgccgattg
    47101 gagaaaccag aagtagtgtt caagaaaaaa gaattaccgt ctttgtttaa agagattatc
    47161 cctctgctgt cagagatcgg cgacgtagct attgcccctg cagtagaaga ggaagtagca
    47221 gatatccccg tcgaatttat tttctttttc cgaaaagcaa aaggaaaaat ccaagctcga
    47281 gtcgatttcg tctatggtga agtcatctat tcgactgacc cgaagcatga ggtctcgaca
    47341 gaacaggctt ttcaggtgtt acgtgacaca gtagaagaga aacgagtact tgatctattc
    47401 agaatgtatc gctaccagaa aaatgaaact ggttatgaac gtccattgcc aagcggggaa
    47461 gaactatatg cctttttccg cacagaacta tctgtcttcc gtcagtttgg tgaagtcaga
    47521 cttggaagaa aattgcgaga gttgtattta gatgcgcata aacatcaacc gaaaatcgaa
    47581 gtaaccgaga ccggttcatg gctcgatatt cgattcgatg tgacagggat agaggagcaa
    47641 gaaatcgatc atgtcctgca aagcttgctg agaaatgatg ctttttatac attagaaaat
    47701 ggtcaggttc tttcttttga ttcagaagaa tttcaacaaa caagccaagt gttgcaacaa
    47761 ttacgggaat cgatacgtac agaagaagga acgatccatg tacctaaaaa tcaaggattg
    47821 atcattcaaa atcaattgga aaaaagcaat gctactttta gtgaatcgtt ccaaacgatg
    47881 gtacaagatc tcattcatcc agaaagatac caggcacagc ttcccaaagg attgaatgca
    47941 acgatgagag attatcaaaa gcaaggtttt cgatggttga agatgttagg tcattatcag
    48001 tttggcggta ttttagccga tgaaatggga cttgggaaaa cactacagac aattgccttt
    48061 ttactttcgg aaaaagaaga acggaaaagc ttctctgctt tgatcgtagc gcctgcgagc
    48121 ttaatttaca actggcaggc agaggtgaga aaattcgccc cttctttaag tatccaagta
    48181 atcaatggga ataaaaaaga acgtgaagaa ttactggcta aagacacaga tatccgagtg
    48241 acttcttatg ccagcttaag acaggatcta gctaattatc agtcgcaaaa aattgattat
    48301 ttgattcttg atgaggcaca aatggtcaaa aatagcagta cgaaaactgc acaagcattg
    48361 agagaattag ctgtacctca gcggtttgca ctgagtggga caccaatcga gaataattta
    48421 gaagaattgt ggtcattgtt tgcaacgatt atgccaggat ttttcccgac taaaacaaaa
    48481 ttcagggaac ttgccgcaga agaaattgcg caaatgattc gaccgtttat tttacggcga
    48541 gataaacaaa ctgtgttgaa agacttgccg gaaaaaacag agatgaatct atattctgca
    48601 cttacagaag aacaaaaaac tgtttattta gcttacttgc gtcaaatgcg tgaagaaatc
    48661 acatcaatgg attctgaagc attcaagaaa aaccggatcg ggatacttgc tggtctgaca
    48721 agactgcgac aaatttgctg cgatcctcgt ctgtttattg aagattacca aggcggttcc
    48781 ggcaagctcg aacaagtaaa agatctactg gtagctgcca aagagaacaa acgaagagtt
    48841 ttgctgtttt ctcaatttac agggatgctg actatcttgc aagaagagtt agccgagcta
    48901 gggatctcga cattttattt aagaggcagt acgaaaccac aagatcggtt aagtatggta
    48961 gacgcattta atgctggcga aaaagatgtt tttctgattt ccttaaaggc tgggggaact
    49021 ggattgaatc tcacgggagc agatacggtc atcttgtatg acctttggtg gaatcctgcc
    49081 attgaagaac aagctgctgg acgtgcacat cggatcggac aaaaaaatgt ggtagaagtg
    49141 tggcgtatga tctctgaagg aacgattgag gaacgcatgg attcactcca gcaagaaaaa
    49201 cgcgaactgt tccaaaaagt gatccaaggt aatgaagaac agttgacgaa actgactgaa
    49261 gaagacatcc gcatgatttt aagtgtagga gaaatcgagg agtaagagac tcgaaaaagc
    49321 gttcagtaat gggtaagaaa gcctttctaa agaagcgatt gtttatttga tttaaaaaat
    49381 agcaaggagg ctgggattcc attttatccc agcttccttg ctatcatttt tttatttcta
    49441 tttttaaagg ttatacaaag attgtcttca ttgtctctaa cagctgatca aaagaagaaa
    49501 tctcgatatc gcaaattgga gtctgttcaa ttttgcgttc tcgatggttg aaccaaagcg
    49561 cttgccagtt agcacttttt gcacctagaa catcattgtc gtaattatcg ccaacgtata
    49621 aggtctcttc tggaagcata tgaaattctt tctcagccag ctgaaaaatc tctttttctg
    49681 gtttttggaa acctgttgct tgagaaatga gcatattgcg ggaaggaatc caattgttca
    49741 attgaagttg cttcagtttt tttgtctgat ggtcagtcgg cccatttgtg ataattccta
    49801 atgggacatt cttttccttc aagaaatcga gggttttttt gacctcctca tgcaagcaga
    49861 tattatcaag ttcttcctcg taaatttttt gaaataagag gccatcttct tccgtaatat
    49921 gagggtagtc taaatctttt agtgattggc tgatccgatg tgctcgcata tattctagtg
    49981 tccaatctcc tgccattact tttgggaagt tttcatcact atgatggcgg aaccggatat
    50041 atagtggatg catatctgca tctgaaacaa gcggaacgac tcttttgacc gcgtttcgaa
    50101 atggctgctg ctgatcatac atcgtatcat caacatcaaa cacaactgat ttaatcaaaa
    50161 ttttacacgc ctttcggttt tcattgattt tttcacaaat ccttgactat tgtaacagaa
    50221 aaaattgtct aaaaaagctg ataaataaag gtttttctta ctatatttct atttccttga
    50281 cagaataagg tttttatatt tcagtttttt tcactaaaaa tcgtgaaaca cgtcaataag
    50341 tttgatgaac aaattaatca ttatggtaga agtcgattga tttattttaa tgcaatctct
    50401 gtggaatttt cg
//
LOCUS       DAJSKS010000005        44329 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJSKS010000005 DAJSKS010000000
VERSION     DAJSKS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.005"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            145..792
                     /locus_tag="ON396_000270"
     CDS             145..792
                     /locus_tag="ON396_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCR3112406.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            807..2333
                     /locus_tag="ON396_000271"
     CDS             807..2333
                     /locus_tag="ON396_000271"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR3112407.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(2382..5180)
                     /gene="ileS"
                     /locus_tag="ON396_000272"
     CDS             complement(2382..5180)
                     /gene="ileS"
                     /locus_tag="ON396_000272"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR3112408.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(5442..6149)
                     /locus_tag="ON396_000273"
     CDS             complement(5442..6149)
                     /locus_tag="ON396_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCR3112409.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            complement(6170..6952)
                     /locus_tag="ON396_000274"
     CDS             complement(6170..6952)
                     /locus_tag="ON396_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCR3112410.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            complement(7001..7288)
                     /locus_tag="ON396_000275"
     CDS             complement(7001..7288)
                     /locus_tag="ON396_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCR3112411.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            complement(7303..7902)
                     /locus_tag="ON396_000276"
     CDS             complement(7303..7902)
                     /locus_tag="ON396_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCR3112412.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            complement(7916..8593)
                     /locus_tag="ON396_000277"
     CDS             complement(7916..8593)
                     /locus_tag="ON396_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCR3112413.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            complement(8610..9851)
                     /gene="ftsZ"
                     /locus_tag="ON396_000278"
     CDS             complement(8610..9851)
                     /gene="ftsZ"
                     /locus_tag="ON396_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR3112414.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            complement(9874..11199)
                     /gene="ftsA"
                     /locus_tag="ON396_000279"
     CDS             complement(9874..11199)
                     /gene="ftsA"
                     /locus_tag="ON396_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR3112415.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            complement(11355..12575)
                     /locus_tag="ON396_000280"
     CDS             complement(11355..12575)
                     /locus_tag="ON396_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCR3112416.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            complement(12590..13678)
                     /gene="murG"
                     /locus_tag="ON396_000281"
     CDS             complement(12590..13678)
                     /gene="murG"
                     /locus_tag="ON396_000281"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR3112417.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            complement(13704..15065)
                     /locus_tag="ON396_000282"
     CDS             complement(13704..15065)
                     /locus_tag="ON396_000282"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR3112418.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            complement(15079..16041)
                     /locus_tag="ON396_000283"
     CDS             complement(15079..16041)
                     /locus_tag="ON396_000283"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR3112419.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            complement(16067..18259)
                     /locus_tag="ON396_000284"
     CDS             complement(16067..18259)
                     /locus_tag="ON396_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCR3112420.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            complement(18260..18661)
                     /gene="ftsL"
                     /locus_tag="ON396_000285"
     CDS             complement(18260..18661)
                     /gene="ftsL"
                     /locus_tag="ON396_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR3112421.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            complement(18666..19625)
                     /gene="rsmH"
                     /locus_tag="ON396_000286"
     CDS             complement(18666..19625)
                     /gene="rsmH"
                     /locus_tag="ON396_000286"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR3112422.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            complement(19646..20077)
                     /gene="mraZ"
                     /locus_tag="ON396_000287"
     CDS             complement(19646..20077)
                     /gene="mraZ"
                     /locus_tag="ON396_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR3112423.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            complement(20247..20615)
                     /locus_tag="ON396_000288"
     CDS             complement(20247..20615)
                     /locus_tag="ON396_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HCR3112424.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            20786..21742
                     /locus_tag="ON396_000289"
     CDS             20786..21742
                     /locus_tag="ON396_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR3112425.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21815..21931
                     /locus_tag="ON396_000290"
     CDS             21815..21931
                     /locus_tag="ON396_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HCR3112426.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(21986..23668)
                     /gene="recN"
                     /locus_tag="ON396_000291"
     CDS             complement(21986..23668)
                     /gene="recN"
                     /locus_tag="ON396_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR3112427.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(23686..24135)
                     /locus_tag="ON396_000292"
     CDS             complement(23686..24135)
                     /locus_tag="ON396_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCR3112428.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(24242..25060)
                     /locus_tag="ON396_000293"
     CDS             complement(24242..25060)
                     /locus_tag="ON396_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HCR3112429.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(25069..25959)
                     /locus_tag="ON396_000294"
     CDS             complement(25069..25959)
                     /locus_tag="ON396_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCR3112430.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(25961..26185)
                     /locus_tag="ON396_000295"
     CDS             complement(25961..26185)
                     /locus_tag="ON396_000295"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR3112431.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(26190..27527)
                     /locus_tag="ON396_000296"
     CDS             complement(26190..27527)
                     /locus_tag="ON396_000296"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR3112432.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(27528..28382)
                     /locus_tag="ON396_000297"
     CDS             complement(27528..28382)
                     /locus_tag="ON396_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HCR3112433.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(28479..28928)
                     /gene="nusB"
                     /locus_tag="ON396_000298"
     CDS             complement(28479..28928)
                     /gene="nusB"
                     /locus_tag="ON396_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR3112434.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(28921..29346)
                     /locus_tag="ON396_000299"
     CDS             complement(28921..29346)
                     /locus_tag="ON396_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3112435.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(29592..30656)
                     /locus_tag="ON396_000300"
     CDS             complement(29592..30656)
                     /locus_tag="ON396_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCR3112436.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(30828..31073)
                     /locus_tag="ON396_000301"
     CDS             complement(30828..31073)
                     /locus_tag="ON396_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3112437.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(31212..31586)
                     /locus_tag="ON396_000302"
     CDS             complement(31212..31586)
                     /locus_tag="ON396_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HCR3112438.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(31669..32583)
                     /locus_tag="ON396_000303"
     CDS             complement(31669..32583)
                     /locus_tag="ON396_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR3112439.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(32604..33407)
                     /locus_tag="ON396_000304"
     CDS             complement(32604..33407)
                     /locus_tag="ON396_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR3112440.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(33440..34429)
                     /gene="manX"
                     /locus_tag="ON396_000305"
     CDS             complement(33440..34429)
                     /gene="manX"
                     /locus_tag="ON396_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR3112441.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(34532..35023)
                     /locus_tag="ON396_000306"
     CDS             complement(34532..35023)
                     /locus_tag="ON396_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3112442.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(35214..38021)
                     /locus_tag="ON396_000307"
     CDS             complement(35214..38021)
                     /locus_tag="ON396_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3112443.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(38455..38748)
                     /gene="rpmA"
                     /locus_tag="ON396_000308"
     CDS             complement(38455..38748)
                     /gene="rpmA"
                     /locus_tag="ON396_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR3112444.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(38761..39105)
                     /locus_tag="ON396_000309"
     CDS             complement(38761..39105)
                     /locus_tag="ON396_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCR3112445.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(39132..39440)
                     /gene="rplU"
                     /locus_tag="ON396_000310"
     CDS             complement(39132..39440)
                     /gene="rplU"
                     /locus_tag="ON396_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR3112446.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(39460..39536)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            39722..40363
                     /locus_tag="ON396_000311"
     CDS             39722..40363
                     /locus_tag="ON396_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR3112447.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(40425..42056)
                     /locus_tag="ON396_000312"
     CDS             complement(40425..42056)
                     /locus_tag="ON396_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HCR3112448.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(42179..42538)
                     /locus_tag="ON396_000313"
     CDS             complement(42179..42538)
                     /locus_tag="ON396_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HCR3112449.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(42697..42770)
                     /locus_tag="ON396_000314"
     tRNA            complement(42697..42770)
                     /locus_tag="ON396_000314"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42734..42736),aa:Arg,seq:tct)
     gene            complement(43079..43762)
                     /locus_tag="ON396_000315"
     CDS             complement(43079..43762)
                     /locus_tag="ON396_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCR3112450.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(43890..44279)
                     /locus_tag="ON396_000316"
     CDS             complement(43890..44279)
                     /locus_tag="ON396_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HCR3112451.1"
                     /translation="MSPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIA
                     VENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTI
                     AFCLTGITAWNTFVELKKKRRRTQKFL"
BASE COUNT        12544 a         9621 c         7499 g        14665 t
ORIGIN      
        1 gaatgagtct tctctatcat ctttcgtttt ttgttgtaaa aacgtctaaa aatcctttgt
       61 tttttattta gaaaaatagt caattaacgt tttttttgtt acacttacta atgatggttt
      121 tttaccatga aaggatgatc gaatatgaca ccaaatcgag aagattattt gaaaatcatt
      181 ttagaactag gtggagatac aacgaaagtc aacaataaac aaatcgtatc tagtctagat
      241 gtctcagctg catctgtcag tgaaatgatt tctaaacttg tgaaagaaga gcttgtagaa
      301 cattcccctt atcaaggtgt acaattgact gcaagcggac tgcaaaaagc tagcagtctg
      361 attcgcaaac atcgcttatg ggaagttttt ttagttgaac atttagacta tagctggaat
      421 gaagtccatg atgatgctga agtattggaa catgtgactt cggaacatct tgcagatcat
      481 ttagaaaact atttgaatca tcctgaacat tgtcctcatg gaggatcgat tcctaaaaaa
      541 gaggaagttg tccatgaaga acgccgacaa acattggagt cctatcctgt cggtacaaaa
      601 gtgcgtatcg cacgagtgct tgatgaaaaa gaattgttgg attacttagt cactattgac
      661 ctgaatatca acgaagaata tgagatcgtt gacatcgcag cttatgaagg accgatcact
      721 atccaaaatg atagaaagaa aatccccgtc agctttaagg cggccagcac gatttttgtc
      781 gataaaatat aaaggagtgt atccacatgt ctaatgaaaa aaatgtattg tttacgatat
      841 tcggcggtac aggtgaccta gcccagcgta aattgtaccc gtctttgttc cgcctttaca
      901 gaaaaggaaa tttaggcgag cattttgctg taatcggaac cgctcgccgt ccctggagcg
      961 atgagcacta ccgtgaagtt gtcaaagaaa caatccgtgc attgaatcct tcagatgaag
     1021 aagcaactac ctttgcaagc catttttact atcaatccca tgatgtaaat gattcaacgc
     1081 attatcagac attaaaagaa ttgtctgacc gattagatgg acactatcaa ttaaacggta
     1141 accgtgtcta ttatttggct atggcacctc aattttttgg aacgatcgtt tctcatttga
     1201 aatcacaaaa tattatgaca aaaaatggct tcaatcgttt gatcatagaa aaaccgttcg
     1261 gttctgatta ccagtctgct tttgaattga accagcaaat cagagaagta ttcccagaac
     1321 aagatatttt caggattgat cattatttag gaaaagaaat gatccaaaac atctcagcta
     1381 tccgttttgc caataatatt tttgaggctc aatggaacaa tcgctatatc gacaatatcc
     1441 aaattacttt tggcgaaaac ctcggtgtag aagatcgcgg gggctactac gatcatagtg
     1501 gcgcacttaa agacatggtg caaaatcata ttttacaggt tgtagcatta cttgcaatgg
     1561 aaccgccagt agcattttcc gaaaaagaaa tccgtacaga aaagatcaaa gcattgaaag
     1621 ctatccgcat ttataacgaa gaagaagtgt tagaaaactt cgttcgcggg caatatgctc
     1681 aaggtgaatt ggatggtgta caattcaagg gctatcgcga agaagacaaa gtggatgctc
     1741 aatcagagac tgaaacattc gtcgcaggca aattcactat cgataacttc cgttggtcag
     1801 gtgttccttt ctatgtacgt acaggcaaac gtttgactga aaaagggact cggatcaaca
     1861 tcgtattcaa acaagttcct gttaatgtat tcaaaacatc tgtcgatgaa ccatgtgacg
     1921 atacgacact tccacctaat gttctaacga tctatattca gccaactgaa ggattttctt
     1981 tatccttgaa tgggaaagaa gtagggcaag gatttgaaac ggaaccaatc aaattggaat
     2041 ttagaaatag tgcagaaatg gttgaaaata gcccagaagc ttacgaaaaa ttactattag
     2101 atgcgttgaa tggtgatgga acgaacttct ctcattggga agaagtcgct caatcttggc
     2161 atatcgtgga tgtcattcga aaagcttggg accaaacaca accggatttt cccaactaca
     2221 aagctggatc aatgggacct aaagcagctt tcgaactact tgaaaaagac ggattcgaat
     2281 ggatctggca accagataac tggtatcgtg aacgtggaaa attaaacgat tgataacaaa
     2341 agggggtgtg acaaagaaca tgtcacaccc ccttttccct tttacttttc ttcgaatcct
     2401 tcagctacag cttcaggata gaagtcttca acgatttttg cacaacgacc gcatagatga
     2461 ggaagttttt catctactcc tacatcttta cgaacttggc gacagcgatc gcaagtttcc
     2521 ccgtctgctt tttccactgt aatagcaaca tcatcaaata ccatagcgtt atctggcact
     2581 tgttcatctg ctgccataac ttcaaagtag tctggtgaaa cgatcaataa ttgtgcaata
     2641 tctgcatcca aagcttttaa catttcacga acttgttgtt ttggataaac agttagtttt
     2701 gcttccaaag atttaccaat caatttgctg tttcttgctt cttccaatgc ttttaagaca
     2761 ttatcacgga agtccatgaa agcagtccag gtatccatca attcttcttg gtttgcaaac
     2821 tcttcataac caggaaactc gcttaactga acgtactcct cttcttcttt caggaatgac
     2881 cagatttctt ctgctgtatg cggaatgata ggagtcaata attttgtcaa agctacagct
     2941 gtttgataga aaacagtttg catgcaacga cgttgataat catcttctgc ttcaatataa
     3001 actacatctt tagcaaaatc caggtagaat gacgatagat caaccgtcaa gaagttcata
     3061 actgtacggt aaatatgcat gaagttatat ttgtcgtacc cttcttctcg gatttctttg
     3121 attacttggt tcaatcgaac ggtcatatat ttgtctacag aacgtaaatc attgtacgaa
     3181 acagtatctt ttttaggatc gaaatcttct gtattcgcta ataagaaacg catcgtattt
     3241 ctgattttac gatatacttc tgccacttga cttaagatat ccatagaaac acgaacatct
     3301 gcttcataat ctacactaga tacccataag cgaaggatat ctgcaccaaa ttgtttgatc
     3361 actttttctg gtgcaatgat gtttcctaaa gatttactca tcttacggcc ttcaccatct
     3421 aaagcaaatc cttgagaaat aactgatttg tatggtgcta caccattgat agccacacta
     3481 gtcgtgatac ttgagttgaa ccagccacga tattggtctg atccttctaa atacatatct
     3541 gctgggaatg ataattctgg tcgctgacgt aaaacagctt catgagaaga accagaatca
     3601 aaccagacat ccatgatatc tttttctttt gtaaattcgc cattaggaga acctgggtgg
     3661 gtaaatcctt caggaagaag ttctttcgct tctttttcaa accagataat tgatccatgt
     3721 tcagcaaata attttgccac atgttcgatt gtttctggtg tgatgattgc ttctccattt
     3781 tcagcataga aaatcggaag tggtactccc catgcacgct gacgggaaat tacccaatcg
     3841 ccgcggtcac ggatcatatt atacaaacgt gttttacccc atgggataac ccactctact
     3901 ttttctactt catccaaaat attctgacgg aatttatcga tagatgcaaa ccattgtgga
     3961 gttgcccgat agatcacagg ctttttcgtc cgccaatcgt gaggataact atgagtaaag
     4021 aaatctagct tcagcaaagc tcctttttct tttaatagat cagtgatcat aggatttgct
     4081 ttgtcataaa agataccttc aaatccaggt gcttcttctg taaacaatcc ttttgagtct
     4141 acaggtgaca ctacagatag tttatatttg ctaccagcga tatagtcatc ttccccatgt
     4201 cctggagcag tatggactaa tccagtacca gcgtctaaag taacatgatc gcctaaaatc
     4261 acaagtgatt cacgttcata gaatggatga cttgcagtca tatattccat ttcttgtcca
     4321 gcaaattctt ttagaactgt tacattttcc caaccgattt ctgattggac tgtttctagc
     4381 aaatctttcg ctacgacaaa cttacggcca tcggcttcca ccaatacata cgtaaatttt
     4441 ggatttacag caataccttg gtttgctggc aatgtccatg gtgttgtcgt ccaaatgaca
     4501 aaagctgtat cttcatcaag aatatctttg ccgtctttga cattgaaagc tacaaaaata
     4561 gatggagatt ttacatcttt atattcaatt tcagcttccg ctaaagaaga ctcacttgac
     4621 ggagaccagt aaattggttt caatcctttg taaatatagc ctttttcagc cattttacca
     4681 aatacgcgta cttgtgcagc ttcataagaa ggatctaatg taacataagg atgttcccaa
     4741 tctcctgcaa ctcctaggcg tttgaaatct gctcgttgct tatctacttg ggaaagtgca
     4801 tattcacgac atttttccaa gtattctgcc atggacattt ctttacgttt cacgccttta
     4861 ttagccaata cttgttcaat cggcaatcca tgtgtatccc atcctggaac atatggtgca
     4921 cgatagccag acattgattt tgaacgaatg ataatgtctt tactgatttt attcaatgaa
     4981 tgtcctaaat ggatattacc attggcatat ggaggtccat catgaagaac aaagctaggt
     5041 tttccttcgt tcaactcttg tcttttttgg tagattcgat tttcttccca ttctttttgc
     5101 cactcgcctt ctcggtttgg caaattacca cgcataggga attttgtttt tcctaattgc
     5161 aaggtttctt tcattttcat tcaaccatac actcctttat ttgttttccg gacacaaaaa
     5221 agcatgttca gccgcaaggg acgaacatgc cgtggtacca cccaaattta gttgcatcta
     5281 tctagtgcaa actctctgtg accaataacg aggtcagccg ctgcttctta ctctaatttc
     5341 ggaaaagcag gtctcaagta gaggatcttt caccatgcag tgtcttaccg aacttccact
     5401 ctcatcggct cgctttaaga ataacaaagt gcttttctgt tctactcgtt ttcattttca
     5461 ccaggcacga tcgagatatc aatagcctgt gtatcaaatg ccgtcatagg tgcttctatg
     5521 actcctgtgt ctgagtttac atcagtatca ttttcattgt caagctgttt tgctaaaact
     5581 tctttgatca cttcatggct gtcagtcacg tagctggaga atggttgtaa aatttcatcc
     5641 cattccggac ttttcacttg ttctaattga gactcaagca tcaagctgat acgttgatgg
     5701 aatactcgtg tttttttctt aagatcttca gtttctgtcg caagctgtct tgctttttct
     5761 gttgcatctg tcaagatttt tcttgctttt tcttctgcat cagttgttaa ttgatgtgca
     5821 cgtttttcag cgttcgcaac taactcatct gctttgtttt gtgcagaagt aacgatcact
     5881 tcggattcct tgcttgcact tgtttttact ttatcagccg tatcttgagc tacgatgatt
     5941 gattggttca aagcatcttt taattcattg aagtactcaa gtttttcttc agaatgtttg
     6001 actgcttttt ctaattctcg atttctttga gtcaattcct catagtcacg aacaactaaa
     6061 tctaagaaat catctacttc gtcttgatta tatccccgca tttttgtcgg gaatgtctta
     6121 ttttggatat ctaatggagt taaagccatg agtaaaccac ctttttattt tatttacgta
     6181 atacaccgaa cagtaccctg tacttttctt ttttcgtctt cccctcaagt tcttggatct
     6241 ggactctgcc ataacctctg actgaaataa tatctaatag ttcaagtaca aaatcaggtc
     6301 ttgtattttc tgtccagttc acttttactt taccagcttc gatcagctgc ttggaccgct
     6361 gtctggaaat attataaatc tcagagatca cactgtctag tcgcatggaa ctaactgttc
     6421 ctctttcttg tgtccagcta tctttcggca taatcatttc tgtgtatttg attggttcca
     6481 agcgtacagc aacctttcca atctttgtta cttgtgcagc aacaaaatga tccacctcat
     6541 ttgcaataaa tacttgccag cgatcaccgt ccgagatgat atcgccaaag taagagcgtt
     6601 tgatccccgt tcccactaaa gttccaagga ttttcccatg acttaggctg gcaaatttgc
     6661 taggataatg gatttcatac aatgctgttt caaaatcttc ctctacaggt tcgtaataat
     6721 cggggaaaat caaacatctt tttctttctg cctgttcata tccgccataa aacatgaaac
     6781 gcaaatcact gctttgtcga atcagtgttt ctaaaatata ggattgtctt gggtcaagaa
     6841 attcggtcag atagggtgca tattgtcctt gaacctgttc aatccaatcg cctaccgaat
     6901 cgataaatgt acgttcatct gcccgaaagt gctggtatac atttgcatcc aaaattttcc
     6961 cctcctatct attctgagaa taaagatttt gttgcttcaa tcaataaact aaatggacag
     7021 ttatccatat aacgatctgt gctaaacctt gtacggctaa gttcaagatc aggattgccc
     7081 ctaaaattgt gaaatccaag ggtccaatcc tcaaaggcag acgatcgaac aaactgatat
     7141 acggttcgca gatcttagct aataatcgtc ctagccctga ttgatacccg cctggaaacc
     7201 aagagagcaa tgcatatacc atcaagataa tgctatagaa ataggctgcc cgttgaagta
     7261 aatgaagtaa tagtgcaata gtataaatcg tagttcctcc tcttacagat caaacaaatt
     7321 cgcatctgca aaagattgag cggttgaact gtcaatctcc atcccagtcg gtgtacaaag
     7381 aaaaatctca tctccaacac gtttgatatc tccatctagt gcataaactg ttcctgttag
     7441 aaaatcaacg attcttctcg cttgtttttc ttctactaaa tgaaaattca ctaaggttgc
     7501 ttctccagca atgattcgtt tagcaatcgc catagcttca gaataagctc ttggttccat
     7561 tacagtgatt tttccagcaa gatttgcttt aggttgtgta gaggttcttg aagctgttct
     7621 ttctttcgct acagtttttg taggtgattg atgtagttca attacttttt tcttttcagc
     7681 cggctgaata ctttcatgcg ttacaggacg tgaagtcgcc gttttttgtt tttcatgagt
     7741 attcgcagca gatacagaat gagttgattg attctgtgtt gtattttttt tagttggctg
     7801 tacagccttt tttgttgttt caggataatc ttggtattcg tactcttcct catcagctaa
     7861 tccgaaaaaa ttagcaattg ctcctctgtt taaaagcggc atttttgttc ctcctttatt
     7921 cgtctttgaa tagcgctgtt cctacacgga taaatgttgc accttcttca atcgcaattt
     7981 gaaagtcttg actcatcccc atacttaatt cactacatgg tgcatgggca taaccttttg
     8041 cttgaacaag atcacgcaat tctttcagcg taccaaacac tttatggatt tgctcatcag
     8101 aagcttcttt aggcgccata gtcatcaacc caacaacctt gatttttgtg caggcagcta
     8161 aagattccac aaacggtaaa agctcatctg gtgaaattcc atgttttgat tcctcaccag
     8221 aaacatttac ttcaataaaa caggataatt ctttctctaa ccgcttctgg atttcttctg
     8281 ctaatcttaa actgtccaac gcatgaaagt aatcaatttc atttacaatc aattttacct
     8341 ttctgcgctg taaattacca attaaatgcc actttatttc aggaaaatca aataaatctt
     8401 gtttcttttc taataattta tctactcggt tttctgcaca attcataacg ccagctctaa
     8461 ccaattcttc agcttgagcg gattcaacag attttgttac agctactaat gtaacttctt
     8521 tcggatcacg atgaacaaga gcacacgaat cctgaatttc ttgcttgatt ttctgcaagt
     8581 tgtcagcaat catgtgctct tcctctcctt tattttttac gacggaaaaa cggtggcgta
     8641 tttaattcat catcgccatg agattttgtt tcttcgcggt tgaatgtatc aaattctttt
     8701 ttttcgatat tgtcaaaatt ggaatcatca actctaggac gaacattttg ttcacgtctg
     8761 atatcccaat cgccaaaact gttttcttct tctgaagaag ttggtttagc ttgatccata
     8821 tctaatacag tcttttgagc aggtgattgc atttgtgctt gtctagctgg tcggctagac
     8881 ttgcgttcct tttttgattc atcgatacct gttgcgatga ccgtcacacg gatttcatcg
     8941 cccatttctt cattgataga tgtacctaga atgatattta catcgccagt tgctgcattt
     9001 gctacgatat ctgaagcatc ttgtgcttcg aacaatgtca tatccaaacc gcctgtgatg
     9061 ttaagaagta cttgttcagc tccatcaata gatgtttcaa gcaatggaga ggagattgct
     9121 tttttcgttg cttctactac gcgatcttct ccactagcca caccgatccc catcaatgct
     9181 gtaccttggt tttccatcac tgttttcacg tcagcaaagt caaggttaac gtaacctggc
     9241 gctgtgatca aatcagagat accttgtaca ccttgtcgta aaacattatc tgcttcacgg
     9301 aaagcttcaa gcatcggtgt ctttttatct acaacttcta ataaacggtt atttgagata
     9361 attaataatg tatccacatt ttctttcaaa cgagcgatac cttctgcagc aaaacggcca
     9421 cgtttcggtc cttcaaaagt gaatggacgt gtcacgacac caacagtcaa tgcgcccaat
     9481 tctttcgcga tacctgcaac gattggtgca gcacctgtac cggttcctcc acccattcct
     9541 gcagtgatga aaatcatatc tgcaccgtct aaagcttcgc gtaaggattg ttcactttct
     9601 tctgccgctt tttgtccaac ttctggctga gaaccagcac ctaaaccacg agtatatttg
     9661 gggcctaatt ggataactgt ttcagctttt gaatttttta atgcttgcac gtctgtgttg
     9721 gctgtaatga attcaacacc tttaacgttt tcttcaatca ttcggtttac agcatttcca
     9781 ccgccaccac cgactccgat gactttgatt actgcgccgt cattgatatt attatcaatt
     9841 gagaattcca tatcttattc ctcccaggat gaattaatca aaaatatttg taaagaaacc
     9901 cttgatttta ttttttacat tttctccgga ctcttttggt tccggctcat cgtagattgt
     9961 ttcttcaggt gcttcatagc tctcatatgt gtttgttgtt tcttgttcaa ctaccatttg
    10021 acgagctgct gaggtttcac ccgtaacagc aatttttgcc aattgataca cttcactcaa
    10081 gtttgctgaa taatccacga tacttattac atttgtaaat accgggtttc tcagccccat
    10141 gtgatttgga acatacaatt ttacatttac gccgaagatt tcttgtgcca agtccacaac
    10201 accaggaaga ctagcagctc cgcctgtcaa tacgacgcca cctggcaact ctaatgcttc
    10261 gatttggtcc aatgcatctt ttgctttgtt gaaaatctgt tccattcgag ctgaaataat
    10321 ttctgacaag tagcgttcat cgactttcac tggttctgac tgaccaatca catctactgg
    10381 aaattcttca tctggtgatg tccgttctgg gtatgcatcc ccgtagttta ttttcaacgc
    10441 ttctgcattg ttaaatgacg tatttaatac aatagagata tctttagtga caaattcgcc
    10501 accttcttgg tcaagattgg taaacttcaa ttgcttatca tgcataacag ccgttgttgt
    10561 ttggccgcca cccatatcaa tgacgatcgt accaaaatct ttttctccgt cagaaagaat
    10621 tgattcagtc agcgctaaag gagtaatcac catttcgttg acgattaatc cggcattttc
    10681 cacgcattta cgtatgttat ggatgatcgt tttaggtcct gtaaacaaca gaccgtacat
    10741 ttctaatctt acgccgatca ttccacgagg atctttgatt ccctcgaacc catcgacagt
    10801 aaaatcttgt ggcaatattg acacgatttg acgttcagga ggtattgaac gaacaagggc
    10861 agctgaagcc acatttctga catcttcatc ggtgatttcc tttgaatctc cgttaactgc
    10921 aatcattccc tggcaatttt ctacttcaag catgtttgct ggcaaaccga cacttacgcc
    10981 tttgatctgg atacctgctt tttcttctgc ttgtcgtact gctcgttgta tcgcttggac
    11041 cgttttatcg atatcaacga taatgcctcg gttaatccct tctgattttg cgtttcctac
    11101 tccaataata ttcatttggc tgtcgatata ttcagccacg acaactttaa cagatgtcgt
    11161 tccaatatca aggcctacat acattcctgt ttttgccatg aatgggattc cctcctactt
    11221 actattccat atacaagcga acaactagcg tccgttttaa aatatactta ttaatagata
    11281 tattcactgc ttccaattct accataaatt actggttttt agaaagtaca ttcttacttt
    11341 ttaaagttgt tttatcaatt ttcatttaaa cttgacggag ttgtacttgg attatcttct
    11401 atttgacttg tttcacttga atcatttgaa tcagatgatc cgtttgtgct agacgatcca
    11461 ggtgtttgtg ctttttgcgt ttctgcctct tcacttgatt cgtttgtttg atccgcattt
    11521 tcttcacttt cagttgtttc ttccgattgg cccgattctt ccgtttcttg agtgctaccg
    11581 taaggatgcg agaagatacc aacttccatg tcgatcacac ctttttcatc catatctttg
    11641 gctacttgcg aataatattt catttggttc gcaagattat tgatatttac aataacttga
    11701 ttgccgtcat tcatattcaa ttggatcaga tttttattgc ttgctcttgg cgtgtacttg
    11761 atttcagaaa ttgctttttg taattctgat gataactgat tgtattgctt tgttaattct
    11821 ttgatttttg cagagtcttt gaactcttcc aagataggga gattttttgt aggatttgct
    11881 actttttcat ctaatactgt tccattttca atcactgggt agtattgatt gttttttgcg
    11941 atcaaagcaa tctctttata ttctgttaca tccaaatcga attcattcat ccctttgaaa
    12001 tgtatatttg ctgtttcgat tcttggctgt gcttttttaa ggcgatcgat ataagtagat
    12061 cgatgaaaat actgtcccca aacattttct cctaatgaca attttgtatc agagatgatt
    12121 tcttggctgt tgactgtctt attgcctgat actgcaactg cttgtaattt gctataagga
    12181 gaaacataat agatcaaaaa aatcagcggt atacccaata cagtgatgat caaagctaac
    12241 cgacgaaata gtactttgtg ccgttgactt tttaaattgg gcagccggtt caaaaaagaa
    12301 ccgttatatg gtccaccgtt tttcttttgc ttgatctctt caaaaaattc gatttttttc
    12361 tcggactggc tttcttcttg ctgttcctct tgaaccgttt tttcttcttc agaatctgaa
    12421 aggggtgtat ctaatgttat gtcttctttt tttggctctt ggtttttcat ctcttgttct
    12481 gccagatatt ttcggttagc cttttgccaa ggagtcaaat tgttttcatc agaagacgga
    12541 tgagacaatt tttttggttc ttttttctta ctcactaaga ccaccgcctt tattttttga
    12601 ttatttgttt tactaagtca taaagacgtt ttgaagcatc agggattcct tgttctttag
    12661 aagctcggca catttgcttt tgcaattctt catcacccat gatttcgttg actgtctgac
    12721 ttagtgattc acctgttagt tcattatctg caatcatttt tgctgctcct gcgtgaacaa
    12781 gactcattgc atttttagtc tgatgatcat tcgtaacata tgggctggga accaacactg
    12841 ccggtaaccc cagagctgtg aattctgcga tcgaagttgc accagcacgc cccaccagca
    12901 gatcactgga agccatgacc tctgccatat ttttgatata aggctggaca ctaatatttt
    12961 gtatttcagg atttatttta tttaggatct cttcataata tctatctcct gaagcataca
    13021 aaacttggta agaagaagga atatattcac ttaaaaaaac tgatactgct tgattgattt
    13081 taagtgctcc ttgactaccg ccaaaaatca agactgtttt cttttctgga tccaaatcaa
    13141 atgttttcaa aatatctgat tttttagtat ctgctacttc ttgtgctcga ggattaccta
    13201 caagaactgc tttgttttca ggaaaaaagg aagccgcatc tttaaatgaa agagcgatcc
    13261 gatccacata tcggcttaaa aatttattgg taacacctgg tacactgttt tgttcatgga
    13321 taactgttgg aatacccatt ttggctgcag cgtacactac cgctccggaa acatatcctc
    13381 cagtaccgat aacgatatct ggttgaaaat cactgaggat tttttttgct tgacggatac
    13441 tcttaagaaa caatcggacc gtcttgatgt tatgcatgga aatttttcgt ttaaatcctt
    13501 gaatctccaa ggtatggaaa ggcattcctg tatcagggac gattttgttt tctagacctc
    13561 gttttgctcc cacatacatg aattcagcat tcggttcttg agtctttaca taattgacaa
    13621 aggcaagagc tggataaata tgtccccctg ttcctccgcc tgtaaccaaa attttcattt
    13681 tttatcgctc gcttctactt tttctatttt aatcggttca cagcttccat gaatctgttt
    13741 cctctagttt caaaatttgg gtattgatcc cagctggcat tcgctggtga taataaaact
    13801 acatcaccat ctgtactgtt ttctaatgct aaaggaactg cttgttctac agaatcggct
    13861 gttactattg tctcgatgcc tgcttttttt ccagcatcca tcaacttttc ttttgtttgg
    13921 ccaaatacga ccattgcttt gattcctttt aatgaaggaa ttaattcatc aaatgtattt
    13981 cctctgtcta atccgccagc caataacacg accttagacg tttcaaatcc acttagagcc
    14041 attttagtcg ctaaaatatt ggttgctttt gaatcattat aaaattttct gccttggatt
    14101 tctcctacat attgcgtacg atgtggtacg ccatggaaaa aatgcaacgt ttctcgtata
    14161 gcttcatttg atataccata caattttgct acagaaatag cagctagtgc attttctaca
    14221 ttatgcttac ctggtacgcc aagttcagat atttccatta ttttttcttt tttatagtat
    14281 attgagtaat cgtcagcata cacaccgtct tccaacactt cttttgtcga gaaaggcagt
    14341 acgcgtgctt ttgttctttg cgctaattct tgtagttctg actgattcca gttaaggatc
    14401 agataatctt tttctgtcat gtttttttga aggttccatt tagctttgac atattctttt
    14461 cttgtaccat gataatcaat atgtgcttca tagatattgg taatcaccgc tatatgaggg
    14521 cggaaatctg tgattcccat gagttggaag ctagacaatt ccatcacaat tttatcttct
    14581 gattttgctt cttgcgctac accacttgct ggatacccaa tattacccgc taaacgtgca
    14641 attccttgat ctgctcctgc attcagtagt agtcctgtca tcgttgttgt cgttgttttt
    14701 ccgttcgttc ctgtaatccc aataattggg cattccgcta cttcgtaagc tagctcaact
    14761 tccgtaatta ccggaatacc catttcttgg gcttttgcta ctaaaggatg actgtaggga
    14821 atccctggat ttttgaccat caatgaaaat tcttcatcca acaattcgat tggatgacta
    14881 cctgttatta cttttatccc taaagaaagc agctcttgtg cttccggatt ttcgtcaaaa
    14941 ggttttccat cattaacggt gacaagtgcg cccaattcat gtaataattt ggccgcactt
    15001 actccgcttt ttgcgagtcc taagacaagc actttcttat tttcatatgt tgatattttt
    15061 ttcatttccc gtcatctcct accaaacgat ccataatgtg atcaacgaag tcacaatgct
    15121 gatcaaccaa aaaactgtat caatcttcca ctccgaccat ccgcacattt caaagtgatg
    15181 atgaattgga gacattttaa aaattcgttt tcctgtaagc ttaaaggaag taacttgaag
    15241 catcacactt gctgtttcca taacatagat caaaccaatc aaaagcaatg tccattcttg
    15301 gtttagcatt atagaaattg ccgctaaaag tccgcccaac gctaatgaac cgacatctcc
    15361 cataaaaatc ttggcaggtt ttctgttgta gacgaagaaa cccagtaaac ctcctaaaac
    15421 acttaagcaa atgattaata catcatactg atgctgatgc caagcaatta gaccgtaagc
    15481 tgcaaaagag atacttccta agccagcaac taatccatcg atgccatctg tcagatttac
    15541 tgcatttgaa aatccaacaa gccagaagag agcgaaaata ccataaatga ttcctaaagg
    15601 tagatctagt ccgaagaaat tcaatacacc tggataacct tcagaacggt aaacaagata
    15661 aaagatgatt ccccctagaa tctgtccaat caatttctgc ttcgagttca gtcccatgtt
    15721 tcttttcttg aatattttta taaaatcatc aagaaagccg attactccgt ataacgctaa
    15781 tacaaaaagc aagatgaaaa gagtaggagt tagctgtttt tgccaagctc caacccaaat
    15841 tcctgtcaaa atagagccga tcagaaacac taagccgccc attgtaggag ttccggcttt
    15901 gctattgtgc cactttggtc cctcctcacg aatcgcctga ccttgctttt tcatttgaaa
    15961 ataaccaata aatagcggca ttgcggcaat tgtcattgca caactgctga caatgggaat
    16021 caatgcttgt gtccattcca tgctcttttc tctcctattt ctcttcttat tcttctaaag
    16081 taaaactaag tttgtcttca gtgatttttt catatggagc aagttcttgt ttgacacaat
    16141 aaccatctcc atcaaaacta acttcaatac cgaggatttt ccccagcttc atgatatctg
    16201 cttttgacca tcctgttacg tcaggcatca gcttatcacc accagtatat aggatcagtt
    16261 tttctccaga gatcaactga tcaccgttgg cagtagattg tttttgtact tttttaccat
    16321 taccgatcac tacaggttgc agtccacttt tttgagcatc agctgctgca acgtccgctt
    16381 caagattacg ataatcggct acggatactt tttctgtttt tgtttctgta tctgaatctt
    16441 cttcagtttc tttaaaatcc attgcccgtt tcatcaatgg atttgcgatt ttagccaatg
    16501 ccattcgatc atatgtctta gggtgtttca ttgttagata caagacatag tctggatctt
    16561 cagaaggaac catctccaca atcgaataca ggtaggctgt atctcctgtt tgatatcccc
    16621 ctgtatcaga agcgatttgg gctgttccgt tttttgccga tacattgtat ccaggtacac
    16681 tatatacacc ataagcacta ccgtagtttt cgctttcgac aacatctctc atatattcac
    16741 ggactttttc tgttgtttct tttgaaaaag gttgccctag aacttctgtt tgagttgttc
    16801 gctcttctcc agtttgggga tccaccactt tgctgatata tcgaggttga atcattgtac
    16861 cgttattcgc aatagaagtg aaagctttca tcatttggaa attcgtcaca cctaccgctt
    16921 gtccataagc actcattgct cggtcgacaa tatttaatgt aggtaaagca ccgtttactt
    16981 catcatctaa tccagaatga gtactttgtc caaatcccag tttttgtaaa tagttatacc
    17041 atcgtccgcc taatcgctgt tccagtatga ccatcccgac gttacttgac catgaaagag
    17101 cttgacgcat ggtcagcaca cctttttcac caaagtcatg gtcattgatc gtagcatctt
    17161 ctacttggat ttttccagat tggaaggttt cattctcgtt gaattctcct tcttcgatag
    17221 cagctgcagt agtgaaaact ttcatggttg atcctggttc ataactatct tgtaccaaga
    17281 aattacgcca gatagcatct tttccagtca atccttccat tgtttcagga ttgaaagtag
    17341 gtcgttgtcc cattgccaag atttctccgg tttttgcttt catcaggact gctgtcaatt
    17401 cttctggctg atattcttca tttacttgat ccatcagtgt ttctaaataa ctttgtagtc
    17461 tgctgtctaa agtcgtgtag atatcttgtc cgtcgacagc tttttcttct tctgccactg
    17521 tacctggcag aggattttga aaattatctt tttgataaat aatcttacca tctttaccgc
    17581 tcaagatatc attgtaagca gactccaaac ctagtttacc aactaagcca ttctcatctt
    17641 tgtctggaac tgcgtatcca ataaaatgag aagaaaagac gccatttgga tacatccgag
    17701 cctgatggtc gacaaaataa agaccagcga caccttcttt tttcatgtcc gcttcgatgt
    17761 tttgcttggt ttcttgggag atatttttcc catagctacc aaattcaact tgatacagct
    17821 ctttttttgc gccgtcctct aaaatattca gtgcatcttt tttgtctata gaaagattct
    17881 tatgcaggat ttctgctatt ttatcaaaat tcttttcttc aacatacagt tttttatccc
    17941 cagaggtata tgtttttgag agtatcgctt taattgaata agaggaggca tcttctgcga
    18001 gcgccacacc gttacgatca taaatcgttc ctctttttgc ttttactact tcacttcctt
    18061 ggtacaactg ttttgttttt tcagctaatg aggtaccagc aacatgacct ccaataacga
    18121 tatacgagaa acggacggca aataaaaaga acaatccaat actcgtagca aataaaatga
    18181 tacctacttt tttgcgattg ttcatcggat ttagattttt cttttccata aaccgccgaa
    18241 ttttattttt aagactcatt tacttcactt tccttaaatt gtcgtcatta atagacaatc
    18301 ctttttcttc tgcgatttgt ttaatccgtt cagtacgaga aagttcgttt ttttcttgtt
    18361 ctaaactcgt tttttcttgc tttttatcat tgatttctgt ttgtattgac gtgatttctt
    18421 tttcagctcc gctgatattt gtgcgcagca taactgttaa aacacataaa gcaacaatag
    18481 caatcgccac gcttgccaca gcaatttttt ccaaagcaga aatctgcttt aatcgttttg
    18541 ccggtgattg tggaagaatg atttcttgat tcgaagaaga atcaggcatt tgttgttccg
    18601 gctgatccac caattcaggt aaatcatatt cgtaatcttg cattttcttt aattctgcca
    18661 tcattctact cctctttcat tcttattttt tctgcaatac gtaactttgc actgcgtgat
    18721 cgattgtttt ctgttagctc ttcctctgaa ggaaggattg gtttacgagt aatgactttc
    18781 agttctggtt ggaattcgtc aggtatgaca ggaagtcccg gtggtagatc ttgtaccgta
    18841 ctatattctt taaacatact tttgacaatt ctgtcttcca atgaatggaa tgtgattacg
    18901 ctgattcttc cattgacttt caataaccgg attgcctgtt ctaaagaggc ctcttcggca
    18961 ccgagttcgt catttactgc aatccgcaca gcttgaaaaa ttctttttgc aggatgcccg
    19021 ccttttcttc ttgcgggagc tgggatgact tgtttaatga tttcgacaag ctcaccagtt
    19081 gtttggattg gttttgtttt tcgtactcgt tcgatttcac gagcaatttg tttggaaaat
    19141 ttctcctcgc catagcggaa gaaaatcttt actaattcat ggtaggaata ttcattaatt
    19201 acgtgataag cggataaagg agctgactga tccatgcgca tatccaacgg tgcatcttga
    19261 tgataactaa atccacgttc tgcttcatct agctgaggag aggaaacccc tagatcatac
    19321 aaaatgccgt ctaccttatc tacatgctca ctcaagcgtt cttctaattc tctgaagttc
    19381 gctttgataa atgtgaccat accttgttcg acataagaag ctaaacgcag cttggcgtga
    19441 tcgattgctt tttgatcttg gtcaaacgca tacagatgcc ctttagaact tagttggctt
    19501 aataaatatt cactatgtcc ggcaccgccg agtgtacagt ctacataaac gccatccggt
    19561 ttgatgttca atccatctac cgtttctttc aacatgactg tatagtgttg aaatgtttca
    19621 gtcattagtc agcctcttcc tattcttata gtccgaaatc aatcatactt tcagcaattt
    19681 cgtcaaagtt ctcttctgct gctgcagaaa aatcttgcca tcgtgtctca tcccaaattt
    19741 caattcggtt agatacaccg atgactacgc aaccttttgt caaagctgca taatttctta
    19801 acgtgctggg tatattgatt cgtccttgtt tatcaagttc gcattcagtg gcagccgaat
    19861 agaagaatcg cacaaaagta cgtgcgtctt ttttagccaa cggcatttcg ttcagtttag
    19921 cttccagatt ttcccattcg ctcatcggat agccgaaaag acagccatcc aatccgcgtg
    19981 tcagaacaaa cttttcgcca agttcttcac gaagttttga gggcacgatt aaacgtcctt
    20041 ttgcatcaat attatgttgg aattcaccca tcaacattga aagcccccca ttcctccact
    20101 aactaaaatt agtctaccac aatcccccac tttctaccac cttttctggg aaatccggga
    20161 acaacttttt ttattcataa acattttttt aaaaatcaag ctgcaaaaag ccttttaaaa
    20221 gactttttac agcttttcta tttttttcat aggtaagaaa gtatgttcac aatgatcaag
    20281 acagcgtaca agacgattgt caacagaaaa accaaacgcc aagtcatctt gaaataacgc
    20341 ggataaataa tttcttcata ataatatgct tggaaaacag caatacaaat tcccattagc
    20401 aaaacagaaa taaagaaaaa cggtaagata gaaattgtcc ctgtatttct agacaatgca
    20461 tgcataccgc cccacaaaaa aggaatcgcc aaatcagggg cttttatttt aaaacgacga
    20521 tttaacgaaa ataattttac aagaaattgg caaccaaaca aaacgattac cgggtaaata
    20581 taccaaaaca agataagtgg tgtgaatgtg cccatgaacg taaactcctt ttttatcgtt
    20641 gcttcaagta tactgtatat actttacaca actgtttcat tttatttaaa tttatgcagt
    20701 tatattctat atcttacaaa cacattccat gtattccaat tccctatccg ttataataaa
    20761 agaaacaggc aacaggagga aaatcatgat caaatatttt acattagata atgagaaact
    20821 gcgtaatacc ggaaaggaaa ctgaccaaac gatctggtat tgtgtagaac gtcctaatca
    20881 agaagagatt catcagctga cacatcaatt tgaaattccg tcagattaca tcacttccat
    20941 attggacgat gcagaaaatt cccgtacaga agaattcaat caatcaaagt tgacaaagcc
    21001 agcgttatta ttgttacaat atccttttgc taaaacaagt cctagcggtt attttcaagt
    21061 ggatacatac ccgctctcta ttattttgac gcccaaaaag aagcttatca caattacgaa
    21121 tcatgaacca ctgtttttaa agaaagtctt tagccagacc tttccaaaaa gtgatatttc
    21181 gcctaatctc aatatcttct ttcaattcat ttggcaggtc acactctctt acaacagtta
    21241 tttagctacg cttcaatctc aatgcgatac tttggaagcc aaacttcaag tatccactga
    21301 aaacaaacaa ctttatcaaa tcatggatat ccaaaaaagc ctcgtttatt tcagagaagc
    21361 gacttctgct aatttagatg cattgaaaga actagcgcaa aataaacttt tccaagaaaa
    21421 tcatgctttg aaaaatcatt tacgtgatgt attggtggaa acagagcagg caatgactac
    21481 ttctcgcatt caattaaaac tagtagaaca aatgaatcag actttttcag caatcgtatc
    21541 gaataatctg aataatatta tgaaaatttt aacttcttta acgattattt taactatacc
    21601 gacaatcgtc ggcagtatct atggaatgaa cattaagtta cctattgctg aaagggatga
    21661 tgcctttata tggctagttc tattaacatt atttatttgt atcgtaacta cctattattt
    21721 aaaaaaaggg aagtttttat aaaaaagccc tcttgtggaa atggaaaaaa gattgtacac
    21781 ttgaatcaac taattaatga cgaggttgaa aacaatgcgt gaaaaaaaac aaacaagtac
    21841 ttttgctaaa gtaacaaaat ttgtcatttg gacaatgctg attttaacga ttggatctgt
    21901 tgtattgact gctgtaatga gtgtcatgta aaaataaaat atagctatca gctgctgaaa
    21961 aggacctgaa ccactgcgag gtggctcagg tcctttttgc gagtttcaat agttcttttg
    22021 catgttcgag tgtgagtttt gtaatctcac ttccagccag catacgtgca atttcttctt
    22081 ctctttcttt tgttttcaac tctgaaacag aagtttgcgt acgtccatca atcactgctt
    22141 tcacaatata atattgatag tcggcgactg cagcaacttg aggcagatga gtgatgcaca
    22201 aaacttgaga gtatttcgat atttgagaaa ttttatctgc gatggcctgt gcaactcttc
    22261 cgctcacacc tgtatccact tcatcaaaaa taatacttgt cacaccttgc tcagaagaaa
    22321 aaatagtttt caaagcaagc aacatccgag acagttcgcc accagaagcg actttcacca
    22381 aaggcttcag tggctctcct ggattggtcg taatgtaaaa ttcgatttgg tcaaatccat
    22441 catgattcaa ttgtctattt tcttctttta agaaacgtac ctcaaactca gtgttttcca
    22501 tatacagact tttcaattca tgcagaatgg atttttccaa ttcttttgcg atttttttac
    22561 gcgcttcatg aagatctgcg gcttgttgct gaagtagaag ctctttttgc tctagttcct
    22621 ctttcatttt gtccaattga ccttctgaaa aatcagaaga agctagctct tcggtgattt
    22681 cgtcatagta agccaatatt gactcaatgg attcgccgta tttacgtttc aactgtcgga
    22741 ttaattctaa acgttgcgta acttcttcta aacgactctc atctaactcc aataaatcaa
    22801 tctgacgact catatcgccg attgcatctt gcagcaaata gtaagcactt tggatatttt
    22861 cataaattgc ttcatactcc acatctagat gggcgatccc ttgcagttct gaaacggcca
    22921 agccaacacc atctacactg ttttgttcat tattcgtcag tgctccgtaa cctgcagcta
    22981 atccatcagc aattttttga taattgctaa gtttttctct ttcttctttt aatttctctt
    23041 cttctccgac ttgcaaatca gcagcagcta tttcttcttg ctggaaatgg agcatatcta
    23101 ttctctgaac ataggatttt tcattttgct gcacttttcg tacccttttt tccagctcac
    23161 gataagagat atattcttgc tgataactct ttttcttctg ttggaaagct acatctccaa
    23221 aacgatccaa taaagcaaga tgtgattcag gctggagcag ttcctgatgt tcgttttgtc
    23281 cttggatatc tactaaataa ctgccgattc ttctcagatt agctaatgtg ataatatgac
    23341 cattcactcg acaaacattt tttccagtta aggacatatc ccgtcgaaca atcagattgt
    23401 cctcatctgt ttcgatccct aattcaacca tcagttcaga gaatccttct tgttttggta
    23461 gttcaaacaa cccttcaagc acacattttt ctgctccttg acgtatataa tcgcttgaac
    23521 ctcttcctcc tgctaacaga cctaaagcat cgataataat ggatttaccc gcaccagtct
    23581 ctccggtcag cgcggtcatt ccttcgtgaa aagaaagccg caattccgga ataatcgcaa
    23641 agttagtaat actgatttct tgtagcatca cattcgctcc tcaacttaaa ggtaatgcag
    23701 gaagtctttc ttcaataaaa ctgcctggtc ttcagttcgt gtaatcatca aaacattatc
    23761 gtcatcattg ataatcgaaa ataattcttt cttgtaacgt ttatcaatca aatttgctgc
    23821 tgccgaagca tttcccggca atgttttcaa aataacaaat ttttccattt ggtcaactgc
    23881 cacgaaagcg tcttttaata gtttttccaa tttagtattt acatcttcgt ttgtttcagc
    23941 tggtatgcta taacggtagc cgccatctga agccggtacc ttgatcagtt tcagttcctt
    24001 tatatctctg gaaatcgtag cttgagtaac agatattcct ttgcttttca ggatttcaac
    24061 aaattcttcc tgctttcgga tatcttgctc actcaacaat cgagtaatca aacgatgccg
    24121 atcttttttt ctcattacgt cttcgctcct tagcatattc ttcttaatca atgatagctt
    24181 atttccttgg tataataaag aagaatccgc atacttctca cttgattttc acagttacaa
    24241 tttatttatg gtcaaactgt tcgtgtgcat ttgaaaccac ttctgccata ttaatagctg
    24301 aattgattgt tccgctttcg ggtactttcc tcaagtgagc caagaactcg atatttcctt
    24361 ctccccccgt gatcggagaa taacttaatt ccagcacatc atatccacct tgtgccgcaa
    24421 atgacaaaat gtcttctaaa actttttgat gggtctgagg gtcacgaaca attccgtttt
    24481 tccctacagc ttctctgcct gcttcgaatt ggggtttgat taaagcaatc acttctccct
    24541 cttcttttaa aatagcatgt aatggaggca gcatcagttt tagtgaaata aaagaaacat
    24601 cgataacagc aacttctgga attcctaagg taaagtcttc gggttttgca taacgaaaat
    24661 tcactctttc cattacgacg actctaggat cttgtcttat tttccaagct agctgattgt
    24721 agccaacgtc taaagcataa ctcatttttg cgccattttg caaagctgca tcagtaaatc
    24781 cgccagtgga tgcaccaata tctaacaatg tttttccttc aacagataga tcaaatgctt
    24841 tgagtgcttt ttctaatttt aatccccctc gagaaacata aggaagtttt ttacctttga
    24901 ctcttaattc actactcact ggaatttttt ctcccggttt atcaaaccgc tcgttttttt
    24961 cgttgtaaat caaaccagcc atcactgaac gtttggcttg ttctcttgtt tcaaataatc
    25021 cttgtttgac cgctaataca tctacgcgtt ctttttccat tctttccctc atttctccac
    25081 atcaaattgc ttgatcatct ggcgcatcag cccgccatca aaagaaaaaa cgtcctcttc
    25141 taatttgtct atgatcttgt tggcggataa cagctcaatc tccaacgcct cacgtgtctt
    25201 ttctaggcct aataaacgcg ggtaagtatt tttttccata cgttcatctt taccagctgt
    25261 tttccctaat gttttcgttg taccaatcac atcgagtaga tcatctctta tctgaaaagc
    25321 taaacctaaa tgttcggcta atcgttgaag tagcaataag atttcttcag gttgcttcgc
    25381 taatatgcca ccagccagca aggcataacg aatcaaatgc cctgtctttc tttcatgaat
    25441 aaatgctaat tcttctaaag aaagcttttt cgattcgccc tcgatatctg cagcttgccc
    25501 agcaaccata ccttgtgatc ccgcacaaac agctaattgc tgaagcagca gaagcttagg
    25561 agaattacct aagtgtgcca tactgattag ctggaatgct cctgtaagca aaccatctcc
    25621 ggccaatata gctaatgctt cgccgaatac tttatgattg gtaggttttc ctctacgaag
    25681 atcatcattg tccatcgcag gcaaatcatc gtggatcaag gaatacgtat gcaccatttc
    25741 taaagcagct gctacttgat agacgggaac atcaataggc tcatcaaagg aagcaacggc
    25801 cgttaaaagt aataagggac gtattctttt cccgcctgct ttcactgaat aactcatcgc
    25861 atcatagagt cctctatccg ttgtatgctc atctaaaaaa gacaagattt ctttttctac
    25921 agcaggtaga tgaatagctg aaaagtcagt taatctcatc tcagctttcc tcgtttccgt
    25981 caaaatcaac ttcttcattt gattctgtca tcattttcgt caaggtctct tctgctttag
    26041 ctaatgtatc ttggcaatgt ttgcttaatt ccattcctcg tttaaacgaa tctagagctg
    26101 attctaatgg aacgtctcct tgttctaact gcatgacaat tttttctaat tcttgcagtg
    26161 actcttcaaa agttggattt tccatatttt tattctcctt tgtctatctc ttgtacttgt
    26221 cctttaacga ttccatcttg ataatggatc gtcagcaaat ccccttgatg aatctcggtg
    26281 acacttttta ctacttgatc atttaacgtc gtatagctgt agcctcttcc catgattttc
    26341 aatggactca acaaatcaag agattgaaca gcttgagcaa aacgctgctg ctgttgtttc
    26401 atatattgtt ggattttctc ttctaagcgt ttttctagat aagcagtttc ttggcgagct
    26461 gctttaactc gataaagcgg agccacttgt tccaattggt ggcttaatgc gatcgtttgt
    26521 ttttctttcg catgataaat ttgctgtgta gcttgatata atcgctgttt caactggtct
    26581 aatttgatgg tctgaccttc atacaatctt tcaggctgcc gaaatacata ggaacgtcga
    26641 gcacgctcga accgttcttg tttgcgctgg atctggcgta caaacgcctg ctgcaatcta
    26701 gcttgccgtt cttgaatgcg catcaactct tctgacaaaa caggaacagc taattctgca
    26761 gctgctgttg gagtggcagc ccgaacatct gctactaaat cagcgatcgt ggtatctgtt
    26821 tcatgtccta cagatgaaat caccggcgta ttagcttgat aaatcgcccg agcaacccgc
    26881 tcttcattaa acggccataa atcctcaatc gaaccacccc cacgaccgat gatcattgta
    26941 tcgaaattgc ccatctcttc tactctctgg atattcctga caacattgtc cgctgcttgg
    27001 tttccttgta caactgttgg gaacaaaacc aattggacaa ttggatatct tcttttcacc
    27061 gtcgtgatga tatcgcggat aaccgctccg cttggactgg taaggaccgc gattcgcttt
    27121 gggaaacgtg gcagcatttt tttaggccct tgaaaaaggc cttccttcat cagcttttct
    27181 tttcgttctt ccaattcttg atataaagca cctattccat caggctccat gtgctctaca
    27241 tagatttgat aagaacctcc cgcttcatat aatgaaatac gcccaacgac aaggactttc
    27301 attccttctt ttggctggaa tcgtaatttt tggaatgctc ctttgaacat aatagcagat
    27361 atttttgcat ggtcatcttt taagctgaaa tattgatggg cattggcacg taaacggaaa
    27421 ttcgatattt cgcctgttaa atatacgcgt tctaaataag gatcggcatc aaattttctt
    27481 ttcaaatact tcgttaatgt agtgacagtt aaatattctt gtgccatcta ttcacctgct
    27541 ccaaaagctt cggcagattt tacggtttga tacatcagca tcgtgatagt cattggccct
    27601 acaccctttg gcacaggagt gatccagcct gcaataggct ccacttcatc aaatttcaca
    27661 tcaccgatca gcttaccgtt agcatcacga ttcatcccta catctatcac tacagcacct
    27721 ggttttacga attcttttgt gacaaaatgt ccacgtccta ttgctacgac taaaatatct
    27781 gcttcttgtg ccagttcagc cagattttcg gttttagaat gagcaacagt aacagttgca
    27841 tctgccatca agagtagctg agccatcggt ttaccgacga tattgcttcg tccgataact
    27901 actgctcgtt tacccgccgg atcgatagaa taagcttcaa acattttcat gatgccgtat
    27961 ggagtacaag gaatcttgtc cggtttaccc gcaaggagtc tgcctaaatt catcggatga
    28021 aaaccgtcta cgtctttttc agggcggata gctaacagga tcttttcttc atctatatgc
    28081 tttggcaatg gtaattggac caatatacca tgaaaactag gatcttgatt gtatttttca
    28141 atttctgcaa gtagttcgtc ttcagaaatc gtctcaggat aacgctctac caatgaatgg
    28201 attccgattt cttttgctac acgttcttta tttcttacat agatttggct ggctggattt
    28261 tcccctacca ataaaacgac taatccggga tggataccat tatctttcaa tttttggatt
    28321 ttttcagcaa tctctgcctg cattttttca gctaattctt ttccgttaat caattctgcc
    28381 atctaaccac tcccagatat tttttgtgtt ttcattctac catagaattt tcccgattta
    28441 taacaggttt atgatgattc atagaaaaaa gctaggactt acgccccagc ttctaattct
    28501 ttcaatacat tggataaaac accgttgacg aatttacgtg aacggtcatc actaaatgtt
    28561 tttgccaact cgattgcttc atttaaagca acagccgctg gtacatctgt cacataagtc
    28621 atttcaaaaa tcgcgatacg caagatcacc aaatccattt ttgagatccg attgatgtgc
    28681 caattgtttt ttaaatgttt ttcaatcact ttatccaatt catctttttt tgcacagaca
    28741 ccgccaacta gcgtatcaag ataaacagga acaaactctg attcatcttc gttgatcatg
    28801 tcgcggtggt ctaattcgat tgcgttgaaa atcgcatctt cttttgttag gtctgcattg
    28861 aagtctaatg gaaataaagc ctgcaatgcc atttcccgaa tctcgtgtct tgataattct
    28921 ttattcattc tcttcctctt cgtcttcaaa aagttcttcg attgttggtt gttctaattt
    28981 ttcaggaaca acagctacta catggatatt gacttcagat aaatcgatat ccgtcatgaa
    29041 aaggacttgc tggctgaccc gttcctgcat ttccattgct actttaggaa ctgaaacacc
    29101 atattctaga taacagtaaa gatctacttt caagccatct tcctcgtttg ctaagtaaac
    29161 accttttccg tgagctgcac gtcctaataa ttcagtgaca ttgctggcaa aagttccgcg
    29221 cattccgtat acgccttcta ctttagaagc tgcaatcccg ataattactt cgatgacttc
    29281 aggagcgatc acgatctctc ctaaatcttg gtttgcgttt aaaatcaaat ttttttcatc
    29341 tgccatttat ttattcctcc ctttgcctaa gcacgagata catccgaatt ctctatgatg
    29401 cggcatagtg atcacgtggt aagaaaactg ccgccgatca gctgcaaact gctatgaatt
    29461 ggagaaaaca cccaacttta ttatctctta cagcttaaaa gttcttctat agtgtatcat
    29521 taaatcgagt gattttctat aacaaagtct gtgaacactg atttttacgc ttacttcttc
    29581 tattgatgaa actataagat gatcaattct tttggtgaac gagtcaacac acgatttcca
    29641 tcttctgtga tcaacaagtc atcctcaata cgtactccac cgattcctgg taagtagatc
    29701 cctggttcat cagtgatcac atttcctggt acaaattgct tttctgcacg agaagaaacg
    29761 tttggtcctt cgtggatctc taaaccgatc ccatgtcctg tagaatgtcc aaatgcttcc
    29821 ccgtaaccat gttttgaaat ataatctcta gcaactgcat ctaattggac accagtcatt
    29881 cctggttttg cagcatcaat aactgctaat tgagcatcta agactatttg ataaatttct
    29941 tttaattttt ccccaggatc accaatagcg aatgtacgtg tcatatcgga aacgtaccct
    30001 tcgtaatagc aaccaaagtc caatgtgatc aagtcacctt gttcgatgat ttttttgctt
    30061 gctacaccat gcggcatagc cgaacgcaat ccgctagcaa cgatcgtatc aaaagaaaca
    30121 cctgaagcgc caagagaacg catataaaag tctagttgat ttgcgacttc aatctcagtc
    30181 atgcccggtt ggatcatttt caggatgtga tcatatgcca tatccgcaat atgacatgct
    30241 ttttcgataa tagcgatttc ttcttcatct ttcacttctc tgagttcttc gatcatctgt
    30301 gaaacaggta ctaattccgc atcgatgatt tcttctaaaa ccgaatattc taaaaatgag
    30361 actgttgttt cctcaaaagc taatgagtgc agttcttctt tgcgtactaa gtttgctacc
    30421 tcatcaaaaa taggtccgac atttttaata atttcaaacc cttgagcctg cgctgccgcc
    30481 tgctcggtat aacgaaaatc agtaataaaa aatgcttttt ccaaagtaat aacagctaat
    30541 cctgtcgtac ctgtaaagtt cgttaagtaa cgaagattgt acggactggt aaccaaaaat
    30601 gaatccaaat tttcttcttg catttttttg cgcaattttt ctacacgtag catcataatg
    30661 aaactccctc tttctttgca cgcttttttc cattatagca aacttttctt cgtacacgac
    30721 agttaaataa aaatttccgg aaaacttctt tttattttaa ataaataaag ccaaaaagga
    30781 gtcacagtct cgctgcaact ccttttagtt tactattttg taaaaactta tgatcgtttt
    30841 ccaaagaaaa atgagacaac tgctaccaaa atcactgcgc ctaaaataga tggaataagt
    30901 gccatacctg cagcttttgg tccccattcg ccgaaaatcg cttgtccaat acttgaaccg
    30961 actagtccag caatgatatt cgttacacag cccatcgaat ttcctttact ggtgatcgca
    31021 cctgcaattg ctccgatcac ggctccaaca atcaatgtcc ataaccaact cataatataa
    31081 cctccttctt tgctaatcaa ttttagcata tgggatttaa aggggacaaa taattggttt
    31141 tgctatttca tattttcttc tgcaaagtaa aaaaataaag aaaacctcct cttagaagtt
    31201 ttctttattt tttacttgcg tttaaacata ctacgaatac cacgtttcac tacatcaaag
    31261 aaactcaatg attgtaccat tctatcttca tctacatatt ctctgatcgc acgaagcact
    31321 tttttaggtt ctttggaaga aaatgtgtat gtaccatttt ttttcgtttg aatggcatat
    31381 cgaggaatcc attttccctt tagcatgact gatgctagta cataatccac ttcttcccat
    31441 ggaatttgga tatatttgcg tctatctcgc gcatggtaaa actcaaaacc attattacct
    31501 atcatgattt gcccgtaatc tgtcagaccc gtaaacgaag tagcatcaat taccaagtct
    31561 acttttgtat tgattgattg aaccattttc tccctccaga tatttgttta tgaacagtat
    31621 actatataaa aaaagctcgg gacaacgccc gagctatatt tttatggatt ataaaagtcc
    31681 gatgacgtgt ccaacaacac cgactacgaa taaaccaagg atgataacga ttggagaaac
    31741 ctttttctta agcaaccaca tacataacaa tgttaatcct aatgcagcta aaccaggaat
    31801 caattggtct aagttgtctt gtaatgttgt taccttttca ggagaaaggg ctaagccatt
    31861 attaacttgt tcaaatgcac tcttgattcc ttcaccacct gaaggcaatt tatcccattc
    31921 gatgtaagca cctttatcta attttacagt tgatacaact ggtaagaatt tgattgatac
    31981 ccatctttgt acaagggcag ctaatacgaa catccctaaa attgaagcac cttttgttac
    32041 gtcttgtaac aatccgccag aaaggtcatc agtaatttta gaaccagctt tgtagccaaa
    32101 ttcttgtgta taccacatga atgcccaacg aataatgttc caacctaaga agaagatgat
    32161 tggtccaagg atatttcctc ccatagctaa tgaagcacct agtgcaccta gcattggacg
    32221 tactgtaaac cagaacactg ggtcaccaac accagctaaa ggacccatca tacctacttt
    32281 aaccccttga atcgctacat catcaacagg cgcgccattc gcacgttctt cttccagagc
    32341 caatgtaacc cctaaaattg gagaagcaat gtatgggtgt gtattaaaga attccaagtg
    32401 acgttttaat gctgcagaac gttcttcttt tgttttgtat aattttttga ttgctggaat
    32461 catagaaaat gcccagccac cgttttgcat acgttcgtag ttccatgaac cttgaatgaa
    32521 ggtagagcgc cacgcaacag ctaaacgatc tttttttgat aatttgattt tttcttctgc
    32581 cattttattt tcctccttca agattaatag tcattcaaaa tgtcgcctaa agggtcacca
    32641 gagttgcttc cgcctccatt atttgatgaa ccacccattt tagaaaggtt caagtaaata
    32701 agagctaatg caacacctaa agcacctaga gcaatcaatg ttaattgaga aattgcagct
    32761 acaacgaaac caataacgaa gaatggccat acttcttttg tagccatcat gttgattacc
    32821 aaagcgtaac ctacagctac gaccattccc ccaccgatag ccataccatc tgttaaccat
    32881 gctggcattg attcaaggaa tgattgcaca gtttgtgctg ggataaatag caacgctgct
    32941 gctgggaccg cgatacgaac cccttgtaaa catacagcaa tgatgtgcca catttcaacc
    33001 ttacggatgt ttccttcttc agcagccgca tccatcaaat gaacgattgg aacagcgatt
    33061 gtacgtacga tcattgttaa gaacaaccct gcaacagcca aaggaacggc aatcgcgatc
    33121 gcagaaggaa cacctttaac accttgtcca cctaaaacta aaataattgc tgatgctact
    33181 gatgctaatg cagcatctgg tgctacagcg gcaccgatat ttgcccaacc taaagcgatc
    33241 atctgaagcg taccgccaag tacgatacca gcttcgaggt taccggtaac taaaccgatc
    33301 aatgtacatg ccactagtgg ttgatgaaat tgaaattcat caagaatacc ttccatacca
    33361 gctagaaagg caactaatac gactaaaatt attgaaataa tagacatgat aagcctccta
    33421 taaattttaa tattatttat tacgcttttg ctaattcagc ttttgctttt ttcaaaatat
    33481 catccatgtt atcgcgtgaa tcattaggta ctttacgtac atcgaatttc acaccttttt
    33541 gttcaagctt ctcaaacgct tcaacgtctt cttgtcccat agacaatact ttacttacaa
    33601 cgactttacc gaccgaatgc gccattgacc caacatttaa ttctttgatg tctacgccac
    33661 cttcaacagc tgtcaatacg tcttcaggat tttcaaataa aagcaatgct ttggtatttc
    33721 caaaacgagg gtcttttgct acttcgatca ttttgctaat aggaataaca tttgctttta
    33781 cacctggagg agcagcttgt tcgattagtc tcttacgcag atcatccttt gatactgcat
    33841 ctgaaacaac gataatacgg tttggttgca ctgcttttgt ccaagctgtc gcaacttgtc
    33901 catgtaacag acgtgagtca acacgtgcta atacatattt gatttttccg tctccgacca
    33961 cagttccttc tggaagtgct cctttaggtt gtgcgtcttc aactgctgct ttcggtgctt
    34021 ctgccggttc taattcttcc ggttttactt ttacaccgtc ttttgctgtt tcaatgatgt
    34081 gtgctgcaat ttcatgagca gattccattg aaaagcgtga tgcgtaggct tcaatcaaca
    34141 ttggtaaatt caaaccgctg acgattgccc atttatcttt gtgctcttcg aataatgtgt
    34201 ttgattgatt gaacggtgtt ccgccccata aatcaactaa gaataacact tcgtcttgat
    34261 tatcaaaaga agcaaccgct tcttccaatt tggctctcaa gtcatctgga ccttcacttg
    34321 gtttcaaaat cacggctttg acgttttctt gttcgccaaa gatcattgaa ccggattgta
    34381 agattccttc agcaaattgt ccatgacttg ctaggataat ccctaccata tttatacctc
    34441 ctatttactt ttgttgtaac aaattcaaaa atattaataa ttgctaacca ttacaacaga
    34501 attatttgac cttttccaac aaccattctt gctacacctt tttgattttc gctagtagtt
    34561 ccatcaaaag gattttcctg tcagcaggta cttttcgaat ctctaactcc acttcttgac
    34621 tgttcaagta acgaaaggaa tcaatatctt gttgatttac tgaaacaaaa ttggtaatca
    34681 ttgatttgcc ttcagtaaaa ctcataccgc cgatattcaa cgacttcaac tgtacaccgc
    34741 agcggacaac cgcttcaaca tcttggggat tggtgaataa aagcatcact tttgctcgtg
    34801 ataataatcc actatgatag atttgaatca ttttctgcac ggttattaca tttgctgtga
    34861 tccccggcgg tgcagcttct tttagtagaa actttctcaa tttatcttga gcgactacat
    34921 cgctgatcac aatgatacgt tcaacacctg tttgcttcga ccaaactgtt gcgacttgcc
    34981 cgtggattaa acgatcatct atacgtgcca atcgaatatc catctgcaaa actccttctt
    35041 cctcactact tcacagaaac ccctatcatt tctgtgtgaa caaccacctc ctagcacaca
    35101 ctactataag caaaaagcgt gccaactttt ttacgataca ctaattgaat ttaaaggatt
    35161 cttaaccaat acacaaaaac gatacactat aaaagtgtat cgtttttgtg tattcatggc
    35221 acagtgtatt cttttttatc taactgatta tggactagac gtagcagata atacatttct
    35281 tctatcggca cttttacttt tatctgatct tccaacagaa caactgaggc tgccaattgg
    35341 ctataaagag gatcattcgt tgtatttgtc aattcgtttt cttccacagt caatggttca
    35401 ttccgaataa tacgttcgat cattcctgct gtgtgcatca ctaggttgat tttgaatcca
    35461 tattcttcat cgccgattcc cgtcgtctga gagatttgtg tagaaaattg ccacattgga
    35521 tcgattaact tactaccatt aataaagatg aagttatcag agataaactc aacacacgta
    35581 tttttagctg actcttcatc tagggagatg aattcagttt cctccaattc tgattccatc
    35641 agcaactgat ccaaaatgac ttcgatattt tgatcaataa aacgttctaa aggaataaat
    35701 ggggcttgga tattcaaatc agtaatccca gtagtagcta tgatctgata ttctttttgg
    35761 ataactggca gttcttcttt cacttccaca atcgacaaag ctagaacttc taattctgtt
    35821 tcttgacgct tgtttaccgc tcgttcgatc aattctttga ttctttgtgc tgtaccttcg
    35881 ccagatgcgc agatagccaa aacagctttt ttcttatgaa taggttttgc ttcctgaaca
    35941 gattctacag aagatatttc tgcgtaacct cgaaaatttt tcaatgattc atgcaacata
    36001 tctagatctg tccctaatac tgagaccttt cgtacagttt ccaaaactag agaagttgtt
    36061 accatatcaa cagtacgtac tggaattcca gtgtctcttt gaatttgcga attaaacgac
    36121 gcaagggacc ccatatctac taagagtatg accccgcttc cttcatctac ttcttgtaca
    36181 ttgcgttcaa ttcggtccaa tgctgttttc ggatccatat ccaaaggcat gtctactgct
    36241 cgtacgttct ctgcatctaa caactgttgg acaacttgga ccatactgct tgctgtgctg
    36301 tttccgtgag ctgcaacgac tacaccgatt ctgccgcttg cttgatttgc ccgtaacgag
    36361 acgagtagaa cggtcaagta atagtcttca ctttctggaa tctccacttg aaaatacgtt
    36421 tcgatataac tacgcacttc tttcgcaaca gcatattcat ctggaaaatc aaaagccatc
    36481 tcacggatgt tatcattcgt atgccgttct tctcctaagt tgattttttt caaaaaagaa
    36541 ctgatatgca agctcatagc ataaataaag ttttgctgaa actcatatgg taaagatctt
    36601 ttgaccatta cataaatctg attggtcact tctatgattt tcggatctac aaattctgct
    36661 aatttgctgt ctgcattaaa agaaaaacca tggtctttat agaaagactt cagatggatg
    36721 ttgatatctg tagaaatgaa atgattgatt gcttcttgat ccaaaccatc cgacttcagt
    36781 aaagctgctt tatctccgat gatatcatat agattataag gtaactcata ggagtccgat
    36841 tggattttca tgatcgtatc atttggtgct acagttatct ttggctccaa tattttagaa
    36901 agctctgcca ttgcactgcg gttgcttgct aattgaatca atccgctacg aatcccttct
    36961 gtcaaatcat cgattgtgat cgagatttcg ggtgattgca tatgatttag aaatcctcgt
    37021 gcacatacta actggatatt tgacttcaat tgtcctatat ttccataggt cacactgccg
    37081 atcaatgctt ttgctacatc ttctgtcaaa gaaatctttc gctgcgtgcg gtttgcttcg
    37141 tgggcaatca tgatttttac taaatcgact ttctcatttg caggacgttt ttcaaaagcc
    37201 ggaagtttga tattgattgg gatacgccga acaaaagttt ctaatagaga agaacctggg
    37261 tcttctgtgg tcgcacctac gatccgtaca tttgcttcat gggatttcgt tgtttcccca
    37321 agacgactgt aagttccatg atccataaag taaaagatca tttcttgacc ttcaggcggc
    37381 aaccggtgaa tctcatccaa aaacagcata ccgccatccg cttgatcgat aatccccatt
    37441 ttttcttcat ctgcgcctgt aaaagcacct tttgcgtacc caaataaatg actcatcaaa
    37501 agctcagggt tattggcata atcagcacaa ttgaaaacaa tcagttccgt ttcctcatct
    37561 attacatgat gcgcttttgc aaaatgatac atcgcgtgtg caaaataagt ctttcctgat
    37621 ccggtaggtc ctgtaatcaa acaatttaat cctcttggcg gatacaaaat cgcagctttt
    37681 gcctgttcca ctgaattttt catgctgccg ttagctccaa tgatttttgc aaagatatcc
    37741 ttagtatcta tttggagtgt atttttttct tcagagaccg gcgtttcttt gtaactgact
    37801 acatgttttg taactggctt tacgtgcgta acgagtgttt gatatatgta acgtactggt
    37861 cgtccgtctg tctttatcag ttttccttcg cgcactagct gattcagatc tttactgcta
    37921 tttgttcgct gaatccctaa cacttcagca atttcctgtg ttgttactcc ttgatcatat
    37981 tctgcctttg ttaaatgagc ggttttttct ttgacataca tataaattcg gtcaatgcgt
    38041 tttgccaatg gtatcgctcc ttttctatag actattgtac ttaggcttca aaacataata
    38101 ctatgtattt atttctgttt cgtcaactat ttacctgatt ttgttttgtt gataacacgc
    38161 ggatagatag aaatgttcaa tttaaaagaa cgaaaaatcc cgatacgatt tttataatgg
    38221 aaacaagaaa aagaacactg tttaccatat aattatttca ctgatagcaa acaatatagc
    38281 ttccatcaat tgaaaacgat agttatcaaa acatcctgat tccaaattgg caaaaaaata
    38341 ttaagtgtaa aaagacaaag taaacttcgt agcctccact acaactttta tttttataaa
    38401 caaaaaaggc tgtgacaaaa gtcttgtcac aacctcttta atcataagat tcaattaagc
    38461 ttcttgagct actggataaa cagacacttg ttttttgtta cggcctttac gttcgaaacg
    38521 tactacgcca tcaacttttg caaataaagt gtcgtctcca ccgataccca cgtttacacc
    38581 tggatagatt tttgtaccgc gttgacggta aagaattgat ccacctgtaa ctgtttgtcc
    38641 atcagcacgt ttagcaccta gacgttttga ttctgagtca cggccgttag atgtagaacc
    38701 gccacctttt ttgtgggcaa ataattgtag attcatagat aacaacatgg tctgcacctc
    38761 ctatttatca ttaattgttt taatttggat atattctgag ttttcagctt ctatcgattg
    38821 taatcctaat aaaagatttt ctaatagaat ctcagcaata ttgttttgtt cttgtgtcat
    38881 tcctgaagtg acttcggtat aaagataacc gccttcttct tcattcattt caacaatcgg
    38941 ctcaaaaccg gctaatgagg caatcccgtt gacagtgctg atcgctaacg cagaaacacc
    39001 cgcacaaacg atatcacttc cataaggacc agcatcagca tgcccggtca aagtgaatga
    39061 aacgatctga cctgcgtcat ttcgcttaaa tgttcctttg atcatatata ggccttcttt
    39121 ctcaaacgct attaagcgtt gatcgcgtcg ataaccactt ttgtatatgg ttgacgatga
    39181 ccttgtttac ggtgtgaatg ttttttaggt ttgtatttga aagtaacgac tttcttttgt
    39241 ttgccgtgtt tttcaacagt accttcaaca gttgcacctg caacagttgg agcgcctact
    39301 tttgtagatt ctccgcctac taagataact tcgtcaaaaa caactttttc tcctgcttca
    39361 acgtcaagtt tttcaacata gattgcttgt cccacttcaa cttttacttg tttaccacca
    39421 gttttaataa ttgcgtacat gctataaagc acctccttct gatatactta gactcgccat
    39481 ccgaagtgac agcatatgct gtgcttatta cttgttctga gcggttgtag ctgtggagtc
    39541 cacaattaca acagtactag cttaccagaa cagcaaccgt tagtcaacta aatttaaaat
    39601 caaaaagtaa aacaaaaatt caagcaatac ttctgctttt ctatcctcac agttctacca
    39661 aataaaatag tcgcattgtg tttcttttat tcttataata aaagaaaaag gaggagcata
    39721 aatggaatac ccaaaagaac tgatacaaga agcttcttta agaatgaacg gccgtcctgt
    39781 agaaggattt atcgctgggc aaggacctct tcatcctaaa ctgatgctcg tgggagaagc
    39841 cccagggaaa acagagattg atacgcatgt tccttttagc ggtcaagctg gaaaagaatt
    39901 gatgcaggca ctttcttcaa ctggcttaac acgagaagag gtgtatatca ccagtgctgt
    39961 aaggagccgc ccgtatcgcg tgactcatcg gatcaataaa cggacacaac agtcagaaac
    40021 tgtttatcca aatcggacac ctactcgttc cgaagtattt gcgcatgcac caatattgga
    40081 ttacgaactg atgaaggtcc agccaaaatt aattgcgaca cttggaaaca ttggtcttca
    40141 acgtttactt ggcaaagaat atacggttac caaaaatcat ggtcaactct atacagggcc
    40201 tgtcttacaa ttaacagaac aaaaggatgc ctacgaatgg tcagaaaaaa actatcgaat
    40261 tcttccactt tttcatccag cagctatttt ctacaatcgc tcccttgtac ctgttatcca
    40321 tcaagactgg caagtactag cttcactttt acagcaatat taaaaagaac agacaagacc
    40381 ataaaatctt cagtttacag tcttgtctgt tctttttcat tgttttaatg gctaattgca
    40441 gtatatttcg catgtaaagc tgtcaacgca tgagcaactg tttgatgttg atgcgtgaat
    40501 gtatgttccc acaagtgaga aagttgtttt tctacttctg gctgcaagga agtatagata
    40561 atcgtttgtc ctctttgctc tgctttttct tgaaattcat caaaaactgc acgtgccgtc
    40621 aaatcaatac ttggtacgcc tcgcatggag aaaatgacac catcgtgatg atttagttct
    40681 tccaatttat ttttgagttt ttctgaagac ataaaaaaca gtggaccact aatatacact
    40741 actgtccagt tattcagctt ttccgtttct ggcagcccca ttctttgcca atcgatcgtt
    40801 tctaatgaaa tcgtgatagc agcgcttttg acaacaaaaa atacacagcc gccgatcacc
    40861 ccgatcacga tagcaatact taaatcaaag ataactgtac aaatcatcgt aaggataaac
    40921 aatagaactg ctgaccaata tttattagta aatagctcct tgatcgtttc ccattcattc
    40981 atacgccaag ccgtcacaat caaaacacct gcaagtgctg gcattggaat ctgtgacatc
    41041 actggcgcaa aaacaagcat ggagagcagt aagacaactg catgtatcat accagctaat
    41101 ctagtttgtg caccagattt gatggccacg cttgtacggg cgatggccgc agtagcagga
    41161 atcccgccaa agaaaggaag caacagattt ccaattcctt gcgccattaa ttcttgattg
    41221 ctatctaagg gcttatttgc cattcggcca gccgaagcac cacatagcaa gctttcaatc
    41281 attcctaata aagcaatgct aatagctgga actgccactt gttgcaaggc agaaaagcta
    41341 aaatctccca tattcaaacg attactactg atcaatgttt gtggaatctt acccacagtt
    41401 gctactggta aatggaacaa tagcataact gctgttgcca aaatgatagc cagcagcgaa
    41461 ctaggcattc gtttcgccca tttttttgga tagataaaca tccctaaaat gactagccct
    41521 cccatgatca aagaggctgt gtttatcgga aatcctatcg tttgataact acttagtttt
    41581 tcaataacgt ttgtaccttc cgagtgaaca ccaaataaat tatcgatctg acctaatgca
    41641 ataatgatag caatacctga agtaaacccc gtaattacgg gtgctgggat aaaagaagtc
    41701 agtgttccta atcggaaaag tcctgctgca agtaaaaata gacccgctaa aaaagtagca
    41761 agcaatacac cttgcattcc ctttgttgcc gcaatagaca tcaagatagc tgccatcgca
    41821 cctgtcggac ctgatatttg ataaaaaccg cctgataaac cgccaataac gataccggca
    41881 atgatagccg taatcaaacc tgcggccgca tcagctccgc ttgatacccc aaaagccaag
    41941 gctaaaggca atgctacagc agctactgtg atcccagcca aaagatcttt ctgaaatctt
    42001 gaaagatcat aacctgaaaa ttcttttttt agtgaggaaa cataattttt gaacacctat
    42061 ctaactcctt tttcatgaaa ttttctaaaa taccattcat tagaatatca tatttcatga
    42121 gataaagtaa tatttttcac aaagtttcct gttagataaa ttcttatttt ttctatattt
    42181 ataagaaaat agaaagcaat gccaacagag ctagcccacc ttgtttgagt aaaatccaac
    42241 gatcgctagt caaactgcca tataatgcaa caaaaaaaat agatattaat agcatacttg
    42301 tgatttcttt agatgcggaa gaaagataag ctccataaag tagtcccact cctaatagtc
    42361 cattgtacac tccttggttc ttaaacaatg tttgaacaga ttttctgctt aactcttctt
    42421 tactcatttt aaaaacccta cttgtcgtgt cagaatcagt tctcaccgtt tcgatataca
    42481 tgatataaaa aaattcaatt gcaactagag tcactaaaat tctagatagt atcgtcatat
    42541 cttcttcctc ttttctgatg agttttattt ttttgctcct tataattgta ctcgataagg
    42601 atgttttttt attttatttg cctttgaaaa caaacaatat tggcacaaaa aacaaagaaa
    42661 aaccgttaga aacctcagtt tctaacggtt ttttagcgtc acaggaggga ttcgaacccc
    42721 cgaccgtccg cttagaaggc ggatgctcta tccagctgag ctactatgac aagaatcagc
    42781 ggaacatcaa taattataga taattaaatt gcttctgtca atacgaaata gttatttaat
    42841 tagcaaataa tgaattattt tggcccaact actaaatcaa tcttttaaaa cgagacattc
    42901 tccttcagct gttctaaggt tatcaattga aaacaggagc tacattgact tcatgctact
    42961 cctgtttatt tagatttcct gtatccttta attgacttac aaatatagaa acttttacat
    43021 caagttcgaa ttgagccctc gattcaatcg attatctaca ggctgccata atcatgtatc
    43081 acaactcgat tgtttctgat tcctttgtca ttaagatcac accatcacct aatggcaaga
    43141 ttgtcgatgt caaatcggga tgatccatca caacacgaag aaattcattt aatttacgat
    43201 ggatagctcg tttgccacgg ggaatctctt catctgggag caagatcgtt cctccttgga
    43261 agatgtcatc taccatcaaa acgccacctt ttcttaacaa acgtaagcat tcaggtaaaa
    43321 attcaatata ttttgatttt gcactatcca taaaaataaa gtcatacgga ccagacaact
    43381 ccggcaagat atctgctgct tgaccttcaa gcaaagtcac tttatctgtc agtcctagac
    43441 gttcatagtt ttttttagct ttctcgatca tgacatcgaa acgatcaatt gtcgtaacat
    43501 gagcgttttc tccgattgtc gtggccatga cacttgatga aaaaccgata gctgctccga
    43561 tctccaagat tcgttcaggc tgaagctgtc caagtaaaaa tttcaaaaat acaactgttt
    43621 catggggaat aactggtact tctgctacat gagcttcttc ttccagtttg cctagctcac
    43681 ccggtaattt tttttgctgg gtacgcataa aatccaatag ttcttctttg actacaggac
    43741 gatgcatcat ttcattgcgc atactactcc tcctcttcac acattatacg aacaactgac
    43801 taaatctaca agctgcatga gtgttttctc gtgtttcatg ttcagaagag tcgttggatc
    43861 tctggaatac atttatgttt ttaacattct tacaaaaatt tctgtgttcg tctccgcttc
    43921 tttttcaact ctacaaaggt attccatgct gtgattcctg ttaggcaaaa agcaatcgta
    43981 aagaataacc cctgaatcca tgcaaatgcc gtttttgaaa ggactagtcc aatatatgga
    44041 atctgaaaga tgggactacc gataatattt ctctttttta ctactccagg ctcaatataa
    44101 gtctttgtat cagcttttgt ataaaaatct tcgtggttgt tttcaacggc tattacgcga
    44161 cgcgtcgaga ttttttttcc tgaattttca taatatgtaa taatatctcc gactaaaatc
    44221 tcatttgtat ttttcggtcg tacatatatc agatttcctt gtgcataata tggactcatt
    44281 tctttgtcct caataatata aggagtcaaa ccaaaaattc ggggaacca
//
LOCUS       DAJSKS010000006        42256 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJSKS010000006 DAJSKS010000000
VERSION     DAJSKS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.006"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            21..245
                     /gene="nrdH"
                     /locus_tag="ON396_000317"
     CDS             21..245
                     /gene="nrdH"
                     /locus_tag="ON396_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR3112452.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            404..2563
                     /gene="nrdE"
                     /locus_tag="ON396_000318"
     CDS             404..2563
                     /gene="nrdE"
                     /locus_tag="ON396_000318"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR3112453.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2613..3578
                     /gene="nrdF"
                     /locus_tag="ON396_000319"
     CDS             2613..3578
                     /gene="nrdF"
                     /locus_tag="ON396_000319"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR3112454.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3667..4806)
                     /locus_tag="ON396_000320"
     CDS             complement(3667..4806)
                     /locus_tag="ON396_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3112455.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5115..5828)
                     /locus_tag="ON396_000321"
     CDS             complement(5115..5828)
                     /locus_tag="ON396_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCR3112456.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6082..6783)
                     /gene="nagB"
                     /locus_tag="ON396_000322"
     CDS             complement(6082..6783)
                     /gene="nagB"
                     /locus_tag="ON396_000322"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR3112457.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7017..8549
                     /locus_tag="ON396_000323"
     CDS             7017..8549
                     /locus_tag="ON396_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR3112458.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8740..9942)
                     /locus_tag="ON396_000324"
     CDS             complement(8740..9942)
                     /locus_tag="ON396_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3112459.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(10024..10713)
                     /locus_tag="ON396_000325"
     CDS             complement(10024..10713)
                     /locus_tag="ON396_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR3112460.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(10819..11187)
                     /gene="rplL"
                     /locus_tag="ON396_000326"
     CDS             complement(10819..11187)
                     /gene="rplL"
                     /locus_tag="ON396_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR3112461.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(11240..11743)
                     /locus_tag="ON396_000327"
     CDS             complement(11240..11743)
                     /locus_tag="ON396_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR3112462.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(11947..12074)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(12153..12842)
                     /gene="rplA"
                     /locus_tag="ON396_000328"
     CDS             complement(12153..12842)
                     /gene="rplA"
                     /locus_tag="ON396_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR3112463.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(12946..13368)
                     /gene="rplK"
                     /locus_tag="ON396_000329"
     CDS             complement(12946..13368)
                     /gene="rplK"
                     /locus_tag="ON396_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR3112464.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            13711..14379
                     /gene="sdaAB"
                     /locus_tag="ON396_000330"
     CDS             13711..14379
                     /gene="sdaAB"
                     /locus_tag="ON396_000330"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCR3112465.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            14392..15264
                     /gene="sdaAA"
                     /locus_tag="ON396_000331"
     CDS             14392..15264
                     /gene="sdaAA"
                     /locus_tag="ON396_000331"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCR3112466.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(15355..16374)
                     /locus_tag="ON396_000332"
     CDS             complement(15355..16374)
                     /locus_tag="ON396_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCR3112467.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(16547..17452)
                     /locus_tag="ON396_000333"
     CDS             complement(16547..17452)
                     /locus_tag="ON396_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR3112468.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(17511..18056)
                     /gene="nusG"
                     /locus_tag="ON396_000334"
     CDS             complement(17511..18056)
                     /gene="nusG"
                     /locus_tag="ON396_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR3112469.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(18157..18327)
                     /gene="secE"
                     /locus_tag="ON396_000335"
     CDS             complement(18157..18327)
                     /gene="secE"
                     /locus_tag="ON396_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR3112470.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(18351..18503)
                     /gene="rpmG"
                     /locus_tag="ON396_000336"
     CDS             complement(18351..18503)
                     /gene="rpmG"
                     /locus_tag="ON396_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR3112471.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            18665..19309
                     /locus_tag="ON396_000337"
     CDS             18665..19309
                     /locus_tag="ON396_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR3112472.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(19365..19868)
                     /locus_tag="ON396_000338"
     CDS             complement(19365..19868)
                     /locus_tag="ON396_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCR3112473.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(19881..19927)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            20465..21430
                     /locus_tag="ON396_000339"
     CDS             20465..21430
                     /locus_tag="ON396_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCR3112474.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(21529..22701)
                     /locus_tag="ON396_000340"
     CDS             complement(21529..22701)
                     /locus_tag="ON396_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3112475.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(22688..23833)
                     /locus_tag="ON396_000341"
     CDS             complement(22688..23833)
                     /locus_tag="ON396_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3112476.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(23847..24771)
                     /locus_tag="ON396_000342"
                     /pseudo
     CDS             complement(23847..24771)
                     /locus_tag="ON396_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            24888..25967
                     /locus_tag="ON396_000343"
     CDS             24888..25967
                     /locus_tag="ON396_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR3112477.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            25999..26592
                     /locus_tag="ON396_000344"
     CDS             25999..26592
                     /locus_tag="ON396_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3112478.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(26702..26929)
                     /locus_tag="ON396_000345"
     CDS             complement(26702..26929)
                     /locus_tag="ON396_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3112479.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(27032..29620)
                     /locus_tag="ON396_000346"
     CDS             complement(27032..29620)
                     /locus_tag="ON396_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCR3112480.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(29640..30299)
                     /locus_tag="ON396_000347"
     CDS             complement(29640..30299)
                     /locus_tag="ON396_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR3112481.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(30348..30851)
                     /gene="trmL"
                     /locus_tag="ON396_000348"
     CDS             complement(30348..30851)
                     /gene="trmL"
                     /locus_tag="ON396_000348"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR3112482.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(31072..31938)
                     /locus_tag="ON396_000349"
     CDS             complement(31072..31938)
                     /locus_tag="ON396_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3112483.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNEVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            32047..32688
                     /locus_tag="ON396_000350"
     CDS             32047..32688
                     /locus_tag="ON396_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR3112484.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(32750..34111)
                     /gene="mgtE"
                     /locus_tag="ON396_000351"
     CDS             complement(32750..34111)
                     /gene="mgtE"
                     /locus_tag="ON396_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCR3112485.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(34129..35028)
                     /locus_tag="ON396_000352"
     CDS             complement(34129..35028)
                     /locus_tag="ON396_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR3112486.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(35030..35827)
                     /locus_tag="ON396_000353"
     CDS             complement(35030..35827)
                     /locus_tag="ON396_000353"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCR3112487.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(35805..36509)
                     /locus_tag="ON396_000354"
     CDS             complement(35805..36509)
                     /locus_tag="ON396_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCR3112488.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            36640..37215
                     /locus_tag="ON396_000355"
     CDS             36640..37215
                     /locus_tag="ON396_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCR3112489.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            37333..38010
                     /locus_tag="ON396_000356"
     CDS             37333..38010
                     /locus_tag="ON396_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCR3112490.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(38089..39897)
                     /gene="pepF"
                     /locus_tag="ON396_000357"
     CDS             complement(38089..39897)
                     /gene="pepF"
                     /locus_tag="ON396_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCR3112491.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(40073..41071)
                     /locus_tag="ON396_000358"
     CDS             complement(40073..41071)
                     /locus_tag="ON396_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HCR3112492.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(41297..41950)
                     /locus_tag="ON396_000359"
     CDS             complement(41297..41950)
                     /locus_tag="ON396_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCR3112493.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        12773 a         8798 c         7466 g        13219 t
ORIGIN      
        1 tgttagagga ggcactcacc atgaacgtaa aaatcttttc taaaaataat tgtatgcaat
       61 gtaagatggt caaacggttt ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg
      121 atgaacagcc tgaagcagta gactggttga aagcacaagg attccaaagt gttcctgtca
      181 ttacttctga tgcagaaaca gttatcggat tccgtcctga tcagttgcgt aaacttgcca
      241 gctaacatga ctaaaagaat caaaacgtga tacatggatt tgaagggctt gagacagcac
      301 cagtttttgt cgcaaacttc caaatccttt tttaaagctt aaaaacaatg caatgatttg
      361 caatcaagca tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt
      421 acaagatgtc agttatttca agctgaataa tgaaatcaat cggccagtca atggtcaaat
      481 ccctttgaat aaggataaag aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa
      541 tacgatgact tttctatcta ttatggaaaa aatcaattac ttgatcgatc aagaatatat
      601 agaagcagaa ttcattcaat tgtattcacc atcatttatt gaaaagctat atcaattttt
      661 ggacgagcaa catttttctt tcaaatcttt tatggctgct tacaaatttt attctcaata
      721 tgcgttgaaa accaacgacg gttccgctta tctggaaact tatgaagatc gagtagcctt
      781 caatgcgctt tactttgcga acggggatga agaattagca cttgctcttg cagacgaaat
      841 gatccatcaa cgatatcagc cagctacacc ttctttcttg aatgctggac gtaaacgtcg
      901 gggcgaattg gtgtcttgtt tcttgactca agtcactgac gatatgaact ctatcgggcg
      961 ctcaatcaac tctgctttgc aattgtcccg tattggtggc ggcgtaggga ttactttatc
     1021 aaatcttcgt gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt
     1081 tccagtcatg aaattattcg aagatagttt cagctattct aatcaattag gtcagcgtca
     1141 aggagctggc gtggtttatt tgaacgtttt ccatccagac attattgctt tcctttcagc
     1201 aaaaaaagaa aatgcagatg aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt
     1261 acctgataaa ttttatgaat tgacacgtaa aaacgaagat atgtatttat ttagtccata
     1321 tagtgtcgaa aaagaatatg gtgtcccatt ctcttatgta aatattacag ctgagtatga
     1381 tcatctagtt gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa
     1441 tgaaatctca aaattacaac aagaatctgg ctatccttac gtaatcaata ttgatacagc
     1501 gaaccgcgag aatccaatcg atggcaagat catcatgagt aatttatgtt cagagatttt
     1561 acaagttcaa acgccttctg tgattaacgg caaacaggaa tatgaagtat taggaacgga
     1621 tatctcatgt aacttaggct cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg
     1681 tcaatctgtg cgtacgatga ctcgtgcatt gacatttatt acagatgcat ctgatatcga
     1741 tgtcgtacca ccgatccaaa acggaaataa gctgaatcat acgattggat taggtgccat
     1801 gggcttacac acttatcttg caaaagaaca aatggaatat ggctcagagg attcattaga
     1861 ctttacaaat atttacttca tgctgttgaa ttattggaca cttgttgaaa gcaacaatat
     1921 cgctcgtgaa agaaaacaag ttttccataa ttttgaaaaa tcagcttatg ctgacggcac
     1981 ttatttcgaa aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt
     2041 gtttgaaggc atttttatcc caactgctga agattggaat gctttgaaac aagcagtaat
     2101 gaaagatgga ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct caatttcata
     2161 tatcaacgat acgagtgcaa gtattcatcc tatcactcgc atgatcgaag aacgccaaga
     2221 gaagaaaatc ggtaaaattt attacccagc gccttatcta tccaatgaga cgattccgta
     2281 ttacacttct gcttatgata tggacatgcg taaagtaatc gatgtttatg caatggcaca
     2341 acaacacgtc gatcaaggca tgagtttgac tttattcatg cgttctgaaa tcccagaagg
     2401 catttatgaa tggaagacaa caacgaagca aacgacacgt gatttgaata ttttaagaca
     2461 ttacgctttc cacaaaggaa tcaaatccat ttattacgta cgtaccttta ctgatgatgc
     2521 agaagaaatc ggaagcaatc aatgtgaaag ctgcgtgatc taatatcata gatagattcg
     2581 cttattcata ttttaattaa aggagcattc tcatgtcaga aacatattat gaagcaataa
     2641 actggaatga aattgaagat atcattgata aatccacttg ggagaagctg acagagcaat
     2701 tttggctaga tacacgtatc ccactttcaa atgacctaga tgattggcgt acattgtctc
     2761 aattggaaaa agatacagta ggacatgtat ttggcggttt gacactgcta gatactgtcc
     2821 aatctgaaag tggcatggat cagttgcgta aagatgtccg cactccccat gaagaagccg
     2881 ttttgaacaa tattcaattc atggaatccg ttcatgccaa aagttattct tctattttta
     2941 gtacactgaa tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat acgaatcctt
     3001 atctgcaaaa aaaagctgag cgtatcaatg aaatctacaa acacggtaca ccgctagaaa
     3061 agaaaattgc cagcgtcttt ttagaaacat tcttgttcta ttcaggtttt tatactcctc
     3121 tttattattt aggaaacaac aaattggcta acgtagcaga gatcatcaaa ctaatcatcc
     3181 gagatgaatc tgtacatgga acctacatcg gttacaaatt ccaattagga ttcaacgagt
     3241 tacctgaagc agaacaagaa acgttaaaag actggatgta caatcttctt tatgagcttt
     3301 acgaaaacga agaacgttat acagaagaat tatacgatcc aatcggctgg acagaagaag
     3361 tcaagacatt cctacgctat aatgccaaca aagcattgat gaacctaggg atggatccac
     3421 tctttccaga tactgccaat gatgtcaacc caatcgtaat gaacgggatt tcaacaggca
     3481 caagcaacca tgacttcttc tcacaagtcg gaaacggcta tttgttaggt catgtagaag
     3541 cgatgaaaga cgacgattat ttgattggat tggaataagg atttatttta attcaaaaat
     3601 ttttcgaaaa ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt
     3661 ttatgcttat tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg
     3721 ttccattctt ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc
     3781 atagacaggt atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa
     3841 taccaagatg atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat
     3901 caaatttcca tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa
     3961 tggagagata aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata
     4021 cgggataata ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa
     4081 tattctcatt gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct
     4141 gatataggca gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat
     4201 cgctttcgga aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa
     4261 tagcaaaata aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat
     4321 ttttgagaca cttgcagata aactgactaa tatgatattg aacaaattac taagagataa
     4381 atttgctgat gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa
     4441 ttcatttaac catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat
     4501 tgtctgttcc attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac
     4561 gaccaaaaac gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac
     4621 aacgattgga tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaactgc
     4681 cgaaagcatt ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc
     4741 gatgatcgtg agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga
     4801 ttgcatacat ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta
     4861 cgcgctgaga ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta
     4921 gacgagtact taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct
     4981 ttttccaaat aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa
     5041 aatataagga actattttta tttggctggg cttattactt gatgtaggac atctatttta
     5101 caacctcaga tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt
     5161 acggacaact cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag
     5221 atatttttgt acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt
     5281 acgatatttt tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata
     5341 aattggattt ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt
     5401 gcaatagcaa tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg
     5461 atccctaatc cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt
     5521 ttcgtgacga tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc
     5581 agccttgctt catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt
     5641 aatgcaccac tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact
     5701 aaaatatgcg tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct
     5761 ccgccaaata gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata
     5821 tcattcatct aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa
     5881 gtgcaatttt tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt
     5941 tatgaataaa cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat
     6001 ggggagctat ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc
     6061 tctttagcct actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac
     6121 aacattgtcg tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat
     6181 catccctttg attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact
     6241 tttcataatt gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc
     6301 gaagaatcgt ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc
     6361 gtcaaatgaa gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat
     6421 ttggatatcg attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag
     6481 atcttctgct tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg
     6541 gacatccatg aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc
     6601 taagtttaca gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa
     6661 agtaactggt gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc
     6721 tttgatgatt tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc
     6781 catagtgatt gctcctttat attttggtat agcccaatta tagcattctt tttttctttt
     6841 tacaataatt agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat
     6901 ccgtgttagg tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca
     6961 agtgattcac tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga
     7021 gtatactgac attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg
     7081 tcgatctgca attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta
     7141 aaagtacatt gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt
     7201 ggcaaaaaaa tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa
     7261 caatcgaaga atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg
     7321 cttttcttta ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga
     7381 aactccaaac ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga
     7441 tgagcagcgg gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg
     7501 gtggacaacg ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga
     7561 tcttagatga accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc
     7621 ttcaggattt tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga
     7681 tcaccaccca tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa
     7741 aacaagcatt gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc
     7801 aacaaaaaca aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat
     7861 caacgatggc aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag
     7921 cttcagcaag taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag
     7981 tggaaacaac tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga
     8041 ccatccgctt tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt
     8101 tattaaaaac actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata
     8161 acacaaaaat caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac
     8221 tacaatattt aagcagtcaa tttccaaaag cgactatcaa agaattacgt cgccaactag
     8281 ctaaagcagg gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg
     8341 aacaaactaa ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg
     8401 atgaaccgac gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga
     8461 aatttccagg aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc
     8521 aacgagtcat tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc
     8581 tgtgacaaaa gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc
     8641 ttcggtatta gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta
     8701 ttacttgatg cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt
     8761 tctctttcct ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc
     8821 gctaaccgtt acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc
     8881 agccgcacct ccaataaagt acataaacat aaatacagta ttatttcggt tgcttgctgc
     8941 ttcacctagc ccttgtactc gtgtttgatt ggcgacttga ccaaattgat tcccgacgtc
     9001 taataaaata atagctaaaa gcaaaatggc caaatggctt ccgccaacca ataaagcaat
     9061 aaagcttatt gtttgcataa caagtcccat atacacgatt tttctttcag aataagaatc
     9121 tgccagtcgt cccacgatag gtgcagcaac tgctccagac aaaccaaaaa tcgctaatat
     9181 ccctgcttcg aatgttcccc aattgtaggc gggactgctg atataaaaaa tcaacgtaga
     9241 ccaaaagatc gaaaatgttc caaacatgaa aaatccactg actgccgctt ctcttaatag
     9301 acgttgagaa gcaactaaat gcggcaaact tttaagtgaa tcaaggtatg aaacagctgt
     9361 actactttgc tggacagaac gcggcatttt ccagtaaagc agaagtgtaa gaattccgac
     9421 tgcacataaa gcaaataaat agatcatccg ccaagaaaaa agactggcaa caataccaga
     9481 taccgtccga gaaagcaaaa tccccacgag taaaccgcta agcaatgtac ccatgacctt
     9541 ccctcttgct tttggtcccg ctagtaccgc accgtaagga atgatgattt gcggcacgat
     9601 cgaaagtaat cctattaaaa aagaagcgca tgcaaataac gtaaacgaag gcgcaaaaaa
     9661 agcggccaac agagagacgg cagataacgc tgccattcga atgatcaatt ttggacgatc
     9721 caccacgtcc cctaaaggaa ctaaaaacag taaccctaac gcatagccta attgggtcaa
     9781 catcgtcaat atcccgactg cacttgtgct aacagataaa ctagctgcta cttttgttcc
     9841 aatcggctgg atgtaataca tattggcaac aacaacgcca caagtgattg ataatagtaa
     9901 aataagcgaa gttgttaaag gttttttttc attactcttc attgatttcc cccatttcct
     9961 atcttagttt acaacttatg gaaaagattg gaaatagaaa aatacaacta ttcgccacac
    10021 ttattatttg tgcttttgtt ttccttgttt tttcttgcga tctttataaa tgaagacaac
    10081 taaccctact agcacagtaa cacttgcaac caacgtgact gcagttttgt tatatccgtg
    10141 aaacagagct tctccaccac acgcatacaa cacagacgaa gggatgactc caatcacgat
    10201 cgacaatatg atttgctgta gaggcaattt catcgcatct gcagcaaaat tgaccataga
    10261 agatggaata accggtatca tatagcctaa cattacacct atttttggat gtttcatttg
    10321 tcggattgct tgcacccaat gattcgtttc ggttttctta tctaaaaatt ttaagcgatg
    10381 cataaggaaa attgcagtga tgttaccaag agcattaccc aaaatgttga tgatcgcacc
    10441 tgtcagtggt ccaaagctca aaccagcaat gactccaact actgaagtag gaatacctgg
    10501 aacagcacac atcaccgctg tcaatacgat caagagtatc gatgcaaaag tcccatgcga
    10561 acgaatatgt gcgagtagct ctgccttact cgctctcgga tttaaaaata attggatgtc
    10621 tgtctgatat tctaaataca accgatagcc cactaccagt atcagaatga ttcccaacga
    10681 gatcaatatc aatcggatta ttttcttttt catatttcct cctgaaaaaa atctatattc
    10741 attaaataat agtttaacat aaaaacaaaa aagcaccgaa atccaacata ggatctcagt
    10801 gcttttaaat agtaaaaatt atttaacagt tacagatgcg ccaacttctt caagtttagc
    10861 tttcaattct tcagcttctt ctttagatac gccttcttta actggtgcag gagcgccatc
    10921 aactacagct ttagcttctt tcaagcctaa accagttgct tcacgaactg ctttgataac
    10981 tttaactttt tggtctccag ctgcagttaa ttctacagtg aattctgttt gttcttcagc
    11041 agcagcagcg ccaccagcag cagctacagc tacaggagca gcagcagata cgccaaattc
    11101 ttcttcaata gctttaacta ggtcgttaag ttctaggata gttgcttctt ttaactcagc
    11161 aacgatgttt tcaatgttca atgccattat aatttcctcc gtttaattgt tcttttttat
    11221 ttaatgcttt atgcggcaat taagctgcat ctccatcttc tttgtctgcc actgctttga
    11281 cagcgtaagc cacgttgcgg actggcgctt gtagtacaga tagcaacata gatagaagtc
    11341 cttcgcgatt tggcaatttt gccaatgctg tgatttcttc caatgaagag actttacctt
    11401 cgatgatacc gcctttgatt tctaatgctt ttgcttcttt agcaaattcg tccatgattt
    11461 tcgctggtgc gactacatct tcgttactga atgcaacagc tgtaggtcca gtgaatactt
    11521 cgtccatgcc ttcaaggcca gcttttttag ctgcacgtga aagaatagaa tttttgataa
    11581 ctttcatttc tacgttagca tcacgaagtt gtttacgtaa acgtgttact tcatcaactg
    11641 ttaatccgcg gtagtcaacg actactactg atgcagctgc tgtaaatttt tcgcttactt
    11701 catcaacgat tgctgctttt tttgcgataa ttgcttcact catttttgag tttcacctcc
    11761 tgaattttga tggaagagtt ttttgcactg aaaaggcaag tcacttttat agttactttg
    11821 ctcaaccagt attattcatc acctattcac ttcgttcata gtgattctac tcgctttgct
    11881 cgaaaggaaa ctcctttcgc tttgctcaac cagtattgtt catcatctct tcacttcgtt
    11941 catagtgatt cacaataaaa aactctatgc caccgcagac atagagggac tattgaatat
    12001 attcaataaa cgtcctcggt aggaaattaa ggcttgatgc cacctactgt cttcggtaca
    12061 agttaattat taacctcaac tagcttagca tgctaaacta gttgagtcaa gaattatttt
    12121 tcagttggat caatcaacat tctaatattt aattagaaag aagcttggtc aacatggata
    12181 ccaggtccaa atgttgttgt gatcgttaca tttttcatgt attgaccttt agctgctgat
    12241 ggtttagctt ttagcaatac atcatggatt gctgcaaagt tttcaactaa tttttcatcg
    12301 tcaaatgaaa cttttccgat tggtacatgg atatttccag ctttgtccac acggtaagtt
    12361 actttaccag ctttcacttc gtttactgct tttgttacgt ccattgtaac tgtaccagtt
    12421 ttagggtttg gcattaagcc ttttggtcct aatactcgtc ctagacgacc tacagtagcc
    12481 atcatatcag gtgttgcaac aactacatca aagtcaaacc atccgttttg gattttttga
    12541 accatgtcgt cgtcacctac gaaatctgca ccagctgctt cagcttcttt tgctttgtct
    12601 cctttagcaa aaactaaaac agtttgtgtt ttaccagttc cgtttggtaa tacaacagca
    12661 ccacggattt gttggtctgc ttttttaggg tctacgttca aacggtatgc aacttcaact
    12721 gttgcatcaa attttgcaat gtttgtttct tttgctaaag ctactgcttc ggcaacagga
    12781 taaactttac ttgaatcaac tttttttaat gcatcttgca tttttttgct ctttttagcc
    12841 attttggttc ctccttgatt gtggttataa cggttgtacc tcccacgtgt tctatatctt
    12901 tcaatataga gccaactgag aagctgctgt cttagggtgt atagattatt ctacagtgat
    12961 ccccatgctt cgtgcagttc cttcaaccat gcgcattgct gcttcaacgt tagctgcgtt
    13021 taggtcttcc atttttaatt cagcgatttc tcttacttga tcgctagaaa ctttcgcaac
    13081 tttatttttg tttggttcgc ctgaaccttt ttcgatttta gctgcttttt tcaatagaac
    13141 agcagctggc ggtgtttttg taacgaatgt gaatgaacgg tcttcgtata cagaaataac
    13201 aaccggaatg atcaaacctg cttgatcagc tgtacgagcg ttgaattctt ttgtgaatcc
    13261 catgatattg atacccgctt gacctagtgc aggacctact ggtggagctg gagttgcttt
    13321 acctgcagga atttgcaatt taacgatttt ttctactttc tttgccacga gacatacctc
    13381 cttaagtccg tgatgtggtt aattggagat gcatatttct cctcccacgt tttatctgat
    13441 ctgaaaaaat ccagacaaat aatgtgcaaa acaatgcaca ctggtacagt atagcaaatt
    13501 tatttgtttt ttcaagattt ttttctgcta tttttacatt ttgcttgaat aaacgctaaa
    13561 tttattctaa aaaaacaaca atttttcgtt ctcatttctt cttttatgtt acacttgcgc
    13621 tagcatggtt caacagatga aagcaaacaa taacacggtt ttttagaccc ttgtacaaag
    13681 attcctacat aacaaaatga ggtggagaaa atgaaagatt taaggtatag aagtgtcttt
    13741 gatattattg gacctgtgat gattggtcca agtagctctc acactgccgg agctgctaga
    13801 atcgggaaaa tcatacgtag tatatttggc gaacagcctg attctgtaga tatttatcta
    13861 tatgaatcat ttgcaaaaac ctatcggggg cacggaacag atattgcatt agttggcggc
    13921 ctgttaggga tggaacccga tgatgaacgt ttagctgatt ctttgaaatt ggcatatgaa
    13981 gccggtatgg aagtcttgtt tgttcctaag agtgaaaaag ccgaacatcc aaactctgta
    14041 aagatgcttc taaaaaaagg aacacgaaag ctttcagtca ctggcatttc gatcggcggt
    14101 ggcaatatcc agatttctga attgaatgga ttcaaaattt ctttaagcat gggtactcct
    14161 accttgatcg ttgtccacca agatgttcca ggtatgattg ctaaagtgac tagtatactt
    14221 tctgagacca atacgaatat cggcactatg acagtgacac gggaatcaaa aggtgaaaat
    14281 gccattatga taattgaagt agatgatcct caagtagaag aaacggtaaa aagactcaaa
    14341 cagctgccaa atatcgacag cgtaaattat ttcgattaag gagctaaaaa aatgttttta
    14401 tcgattgaag aattagtaga tcaagcaaaa gcttatccaa gtgtagcaga attgatgatt
    14461 gcagttgaaa tggaaatgag tggccgttcc cgtagtcaca tcatcgagat catggaaaaa
    14521 aacctctcag taatggaaca atcaatcaac gagggaaaag aaggtgttac ctccgtcaca
    14581 ggaattaccg gtggtgatgc aaaaaaatta gatgattatt taaatgaggc agacttttta
    14641 agtggagaaa caatcttaaa agctgtacgc aatgctgtag ctgttaatga agtcaatgca
    14701 aaaatggggt tgatttgtgc tacaccgact gccggaagcg cgggtgttgt tcctggtgtg
    14761 ctgatggctg tccgagatcg gcttgatttg acacatgaac agcaattgga attcttattt
    14821 accgctggcg ctttcgggtt agtcatcgca aacaacgctt ctatcagtgg tgcagaaggg
    14881 ggatgtcaag cagaagttgg ttctgcaagt gcaatggcag cagcagcttt ggtttgtgca
    14941 aaaaatggga ctcctcatca agcagcacaa gcagtagcca ttacattgaa aaatatgatg
    15001 ggactgattt gcgatcccgt tgcaggctta gttgaagtcc cgtgtgtcaa acgtaatgct
    15061 ttaggctctt ctcaagcttt tatctctgct gatatggcat tagcgggtat cgaaagtgtc
    15121 attccgccgg atgaagtcgt tgctgcaatg taccaagtcg gacgtcaaat gccgtcgatt
    15181 ttcaaagaaa cagcagaagg tggcttagct gttacgccaa ctgctaaacg acttgcggaa
    15241 gagattttaa ataagagggc gtgataaagc tccttaattt cgagtattaa gagattagtt
    15301 atcttcataa caaaaaacga agagaggact aattgccctt ctcttcgttt tttattaatc
    15361 catcaatttc gtatttttta cttcatcgat cgtttttgtt cctgcaagtt gcatcgtgat
    15421 cgacaattct ttgttcaggt gttcaaaaac agatgttact ccttctgccc cacctagatt
    15481 caacccataa atcactggtc ggccaatcgc aactaagtca gcaccacttg ccagagcttt
    15541 gaagacatgc tctcctcgac ggacaccaga gtcgaaaatc actggtacac gtttgttgac
    15601 tacttcggca atttttggca atacttcaaa ggacgcagga cctccatcta gttgacgtcc
    15661 accatgattg gatacccaga taccatctgc ccctgcagag atagcgatta ctgcatcttc
    15721 tggcgattgt attcctttta cgataactgg aagatgggtg atttctttga ttgttttgat
    15781 gtcatctggt gtcaagcctt gttttgcagc tgcataaatt tctgcgattc cttttccttc
    15841 gccattacca cttgcacttt gttcgctata cgcagctaag ttaggcattg gtaatggaaa
    15901 ttggaattga ttgatgacgt cttcttcacg gtaaccaccg agcgttgaat ctgccgttaa
    15961 aatgatagct ttagcccctg cttctactgc tttgttcaaa atgaactcat tgaatctgtc
    16021 gtctttactc atatataatt ggaaaaactg cggtgcgcct ggcgctgcat cggctgcgtc
    16081 tttgattgtc gtatttgcat aagtgctgat ggaaaagatt gatccagcag cagctactcc
    16141 tttagctgta tcagcttccc ctttttcatg tgccaatcct tgggccgctg atggcgcttg
    16201 gataatcggt gtatctaatt caattccgaa aacagatgta tgcaaatcgg ctgagtcgat
    16261 tccgcgtaat atccgtggca tgattttctt tgtattaaaa gaagtcgtat tttctttcat
    16321 cgtccactca tcttcagaac caccgcgaat atagccaaaa gctcctgctt ccatgcgctc
    16381 ttttacacga ccttctagtt aggcaatgtt tacaatatcg attggatgtt cttcattact
    16441 tgtatgataa gtcatagtag atccttcttt catcttcttg attacatata aatcatacta
    16501 aacttacaaa aagtaagcaa acatttactc tcagatactt ttatcgctat tctttccaaa
    16561 taaatcgact tacaagctga tctacttctt ggttttcatg aagcatacta tgagaggcat
    16621 tttcacctgt cactactttt tcctctattt catttccgcg ttgcttcaac aacgcataga
    16681 ccgctaatgc actgtttaac ggtactgttc catcactcaa gtctgtttca tctagttgac
    16741 cagcaatcaa gaaaaatcga gtggttatag gaatattacc gatcatttgc tggtaatctt
    16801 gatagcggtt agaaacgaca gcaggcccct ttgctaactc atctgtcaga gactgcgcac
    16861 tatcatcgac aaaatcatta aaaggcgcgc caattgctgt taatgtctga acttgtggca
    16921 atgacgtatc ctgtccatac gttcctaaat agcgtagtgc actaactcct cccatggaat
    16981 gccccactat gttgacttta tctatgccgt agttttcttt cagatacaaa agacaagttt
    17041 tgatccattc tgcttgattc cactcgttat ttttattatc ttcaaacaat acttggacca
    17101 tcggattgtc tgcttttcca gacaaatctc ctgttgcttg tatttgtcca tctgttccta
    17161 ctgttaaaac caattctttt actgcttgct gatttttttc caaacgccgc aacatccctt
    17221 taaaggaatt ttctgtcccg ctgtagccgt ggaaaaaaag tgttggaata gtggtttttt
    17281 ctatcacttt actggattct gccgtttttt ctttatcaga aacttcagta gtatttatct
    17341 tcgtcgtctg gcaaccactt aacgcaaaca aactgataag gactaaagct gctgcagttg
    17401 cacgtttgca taaatttttg attggttctg tcatacacct cttcctttcc atatagaaaa
    17461 aagaaagact tgaaaggcca atcatatgat cgacatttca agtctcttta ttaatcgatt
    17521 ttatctactt gatcgaaatc aagttctgta ctggtttcac gtccaaacat atcgatgttg
    17581 actttcaatt tttctttttc tccgtcaact tctgttactt gtccttctaa tccagcaaat
    17641 gcgccttcga tgattttcac gacttcgcca acttctactt ctagatcatt ttgtcgcagg
    17701 ctcataccga tagaacgcaa gatatgattg acttcttctg gaagtaatgg tgctggtttg
    17761 cttccggctc cgtgtgaacc aacgaatcct gtcacaccag gtgtgttgcg gacaacatac
    17821 catgaagcgt ctgtcatgat catttcaaca agtacgtatc ctgggaatgt tttatgaacg
    17881 atttcttttt ctttcccatt tttgacttca gtttcttttt cttcaggaac aacgacacgg
    17941 aaaataaagt cggccatttt catactttgc gcacgagatt cgatatttgc ttttactttg
    18001 ttttcatatc ctgaatatgt atgcaagaca taccagtttt tttcaaaaga ttccattgaa
    18061 ggctcctcat ttcattgtaa aataaaaaaa cctcagtaca cccgaagttt ttctttcgct
    18121 tcattatagc actaaaagta cgttgtggct agcgagttat tttaaaatcc agccaaataa
    18181 gctttggatg gctgtatcca ttacgtaaaa catcaatccg aaaataatag aagtttcgat
    18241 gactactaac gtatctttac ggagttgttt tctgcttggc caagttactt gtttgatttc
    18301 atcttttacg ctttttagaa atttcatgag acaacctccc ttgctactgt ttatttcgtt
    18361 tctttatgta atgtatattg attacaatat ttacaaaact tattgatttc caaacgttcg
    18421 ccgctttttc cttcactgac tgattttgtg tagttgcgag acccacaaac ggaacaagcg
    18481 agtgctgctt ttttctttgc catttacttc tgcccccttg ctgagaatca gacttatact
    18541 tcataacagt atcacgtaac tttttacttg tcaatttgtg aaataaacta tctattgttt
    18601 tggataaaat tatgatatga ttaatttgaa attaagctaa aatgagacaa ttggaggtat
    18661 agcgatgcta ttaaaaacac ttgtatataa gaaaaaagat ctgacaaccg ttacagagac
    18721 tagtacatta gaagaagcat taaaaatttt agaaacttcc ggataccgtt gtgtgccgat
    18781 cttagattct tcaggtaata ttttccgtgg aaacatttac aaaatgcata tctatcgtca
    18841 taaagcaaat ggcggtgata tgacgcttcc tgtcactcat cttttgaaaa acgctacaaa
    18901 gttcatttac ctggatactt ctttcttcaa agtattcttt acgatcaaag agcttcctta
    18961 tattgctgtc cttgacagtg aaaatcattt ttatggtatt ttgacacata gtacgttgct
    19021 taatatgctc tctcaatctt ggagcgtaga acaaggaagc tatgtactga caattgcttc
    19081 cacaggaaag caaggagatt tggctgctat tgcaaaaatc atttcaaaac acagcagcat
    19141 tgccagctgt attactcttg atatcgaaaa agacgaatac attcgacgta cattgatcac
    19201 gctaccggct aacactgaag cgaaagtatg tgaaacgatc gtcaaccact tagagaaaaa
    19261 gaattataaa gtggtagaaa ttgaaaattt gcaaaaccaa gaagcataat caaaaagcga
    19321 aaaaagaaaa cagctaatct gctttctttt ttcgcttttt ataattagac acgcttttca
    19381 aaatcaagcg cgcgatctaa ataatacatc aatggtgctg aaacagccat gacgataaac
    19441 tcacttaatg caagcgtgcc ataggttgtc caaaatgctt ggccacctgt tggaactaac
    19501 atataagcaa tcagtcccat gctgatggta aagaagatcg tgttcaatac taaacgcatc
    19561 tttacatttt tcactttgtt ttgcaataaa gctgtcaggc ctaaagaaag caatgtttgg
    19621 ccgccaccaa aaaccacatc catgatacca aaaccaaaga aggcattata gacgatgacc
    19681 ccgcctaaaa ctccccaaag caatttacgg ttaaagacaa ctaaatgatt caaactttct
    19741 gaaatacgaa actggatcgg ccctgaggat acaggcgcaa ctaaaatagt taacgctaga
    19801 taaagagcca tgatgatgcc atttacaacg attacggata accgttgtct gcttttcgtt
    19861 ttttgcattt tctctcctct tttctcctag tttttttaac gcgggatggt tgatgaaccg
    19921 cggttatgtg aaacaaacaa taattataac agacttttca tttatttcaa tccgtcttat
    19981 ctgcttttaa aaaagcaaaa gcttaaacaa tgtaaacgtt tgctaaaaat cgtgggatat
    20041 ggtataaagt aagtgtaagg ggtcataaga attcccagta aagcagacga agtaggatct
    20101 gcaagaacag aattgttgtg aaacttaaat aaataaaaaa ggatgtggga atttgagcaa
    20161 cctcttacta gagaacggtc tagcaagcag ctgaagatct aagtggacat gccgaacctt
    20221 ggaacttctc cttacgtaag taatggatca tgcgcttgct aggctttttt gtgtttttcg
    20281 gtcttcggac ctttttcatt ttcctagact tctgctataa taaccaagaa ttaattttgt
    20341 aacagatttt tttgaaaaaa caagtttctt tcattcaaaa aatagaaaaa gcttgctaat
    20401 tattggttaa ccagcaaaca ggagtattaa aatatcttgt tttattgaat agcaggagga
    20461 aatcatgaaa ttccattatc atatattgat caatccatcg gcaggcagcg gaaatggcta
    20521 taaagtagca gaaagaattt taccggtatt aaaaaataag catattgact atacacttca
    20581 ttatagtgaa tacaaaggac acgatgctga gattgctgaa actttggcaa aagaaacatt
    20641 gattccttgg atagaagaag aacagaaaac aatcgatact ttcccccttt tgatcatcgt
    20701 aggaggtgac ggcaccttac atcaggtact tgatactttc tatcagatgg aagtagaatt
    20761 cccagttgct tatattccag caggttctgg agatgatttt gctcgagggg caggccttcc
    20821 aaaaaatccc aaaaaaggac ttcagctgat tttagcagct caatcaccag aaaaagtcca
    20881 cataatggct tatgaagaaa aaataagtga aaaaaaagga atagctgtta ataatttcgg
    20941 tataggatta gatgcagcta tcgtccatgc gacaaatcat tcttccacaa aaaagcgatt
    21001 gaacaaatac aacttaggat cattttcgta tttattctct attctccgtg ctttattcac
    21061 tcaaaaagga tttccaattc tggtagatgc aggaggaaaa caattaagtt tttcaaacgc
    21121 atttttatgt acagcaacga agcatccttt ttttggcgga ggaatcgcga ttgttccaac
    21181 tgctgatgcg tctaaaccag tcattgattt tgtcgtggtc gaacgaatca atttctttaa
    21241 aatcttgtgg ctcattcttt tacttataca gaaaaaacaa ttgaaatcga aatatttcca
    21301 tcactacaca acaaacagat tacgtatcgt ttcaacaaca ccgcaacatg gtcaagaaga
    21361 tggagaagaa atggaaaaac gaccttttga tatcacaata accacaacta aacagttgtt
    21421 ttggtgttaa taaaagaggt tttgtcagaa gctacatctg gaacaccgtt tattcggaaa
    21481 aaaggactgt gacacgactt ttgtcacagt ccttttttta ctatgcagtt aattctttgc
    21541 ccaattgggt tgccatcgat agtttctttc tttacccaaa ataaattgga tcattgaaaa
    21601 caccgcagca aaagcaacta agacacaacc attgaaccaa aaagattctg caaatgtttg
    21661 attgtaactg agtgtttcac tgatcaagtt attattttga acaaaccaat atgttggtgt
    21721 gaatgccgct attttgctaa ccatttctgg caagtattct gctggtacaa acaccccacc
    21781 gataaagcaa ctgcctgtga taaaaatatc tttgatccca ccgctggttt ctgagttttt
    21841 taccaatgaa gtgatacaag ccgaaaagct aatgatcggc aataaaaaca agaaagtatt
    21901 taaaatatga taccataccg ttatatccca cgaggtgtgg atcactaaca gaaccattag
    21961 aagtaagaag ccactgaaca acagcagcga gtaactaatc aaactaaagg ttactttgcg
    22021 attcaagttt cgtttggtaa tgggcgcact ctggtttcgt ttactgatct ctttgcgatt
    22081 aaatgctaga ttgatgacgc tgaaaccgct aaaaatggat agaaacaacc cataagacaa
    22141 gaaattaaag gttgtgcccg tcaacagctt tttcatccgc tgactatatc ccgcacttag
    22201 ctcaacttct ccagtaaccg agagtgccat ttgtgtctgt tccatagctg catcgattgt
    22261 tttatcttct tcttgaaaca gaatcagtgt ttgcaaaaag gtgttcactt gctgtgtaac
    22321 aactgtttta gaaaaggcat ctgggcgact ctgtatcttg atcgtcggca tctttcctac
    22381 ttcgacttgt tcggtaaagt ctgcaggcag atgtaagata atgtttacac gattaaaata
    22441 aagagcatca tcgatttctt tttgtgaggt tccatccaat gtaacgactg tttccttgtc
    22501 attcaaatat ttacgaaggc catcgactac aggcgactct tcttcactaa tcaaggcgat
    22561 tttcgcatct tgcagagagg tattttcaac atcaatattt tgcgcaaaaa agaatgtggc
    22621 aacaacagtg ataattacac ctatcagtaa attcgattta tttgctttaa gcaatgtcca
    22681 aatcgtttta taaagcgtca taacgcaccc ctctttccat ccaagcattt cctaacgcaa
    22741 aaaggaaaat aaatccgatc aaccatagaa gattggtata aaaactagcc aaatcagtgt
    22801 agaaataaag tttatacaaa ctttcgctga tcaagttgac gagatttaat tgaccaacaa
    22861 tcggaatatg aagatcaatc caatacttca cttgttccga ccccatcata ccagcaaaaa
    22921 agctcatcgc aatcgtcact gaaatcaaga tgctatcctt ttgcaccaga ttcatttttg
    22981 gtatcaaatt accaaccaat gtacctaaca aaatcgcaca caacgacccc agagcggtaa
    23041 caatcatcaa ccattgccaa cgttcaccaa agtctacttg atagataaaa cgaaagacag
    23101 ctaacataat ataaatctct gtcaaaacaa tcgtaaagct tgctgccaaa ttagcgattg
    23161 aaactaacga ttgctttcta ggaatcaagg acaagcgaat accatttgac gattgatttg
    23221 cttgctgatc ctgtacattg cggacccccc acatcgtccc gtagagaatc gccattccaa
    23281 tcagagtgaa gaaataaaaa cttttgaaac tcatattacg acctgcatga acttcttgaa
    23341 aagatacttc ttgttcaaaa acagtcatga tgggcgcgag atcaacgttc tccacttctg
    23401 ccactttact cgaaattata ccaatacgct gtaaaaattg atccatgacg tttttcatca
    23461 acgtctgact cattcctgct tgacccacat ggagcgaaac agatgaacca tcaatgagat
    23521 aaaaaccagc caaatcatct gcatttagct tttcattcgc ttctacttcc gatagttcct
    23581 gtacttcaaa taatggttta tcattgactt cgacttgttc taagactgtg gtaaaatttt
    23641 ccgaaagcgc tgtttgatcc gctttgatta caattccagt attaatcgtt tcgagattgc
    23701 caccttgatc aatctcgcca aaggccgcca taaacatcaa tcctaaaaga attggaaagg
    23761 ccaatgtcca aaacaacatg gatttatttc gcagcagtat ttttgtgcga tatttataaa
    23821 aatgccaaaa cattatttcc cctcctctag tctctcaaat ccttgccagt aatctctaaa
    23881 aagacatcat tcaaggacgc ttctttggtt gctatttttc cgtaactgat cttttcttct
    23941 tgtaagattg taagaatcgg caacaatact gtatttagtt cagtagttgt tacttggagc
    24001 tgaccttttt tataagcgat tccatcagaa gctattgttc gctgacaaca cgttttctgg
    24061 gagtaatggg atgtcgatcg tcaaacgatc cgccgtccgt accatatctt ttagagattc
    24121 tttggttcct ttagcaatca cctgtccttg atccatgatc acgatctggt cgcaaagaat
    24181 ttctacttct tccatgtaat gtgttgtata gacaatggtt gctccttgtc gattcagctc
    24241 cttgatactt tctaaaattt tattccgact ttgggggtct acagctactg tcggctcatc
    24301 taaaaaaatt aaccgcggct tgtgggcaat cccacatgcg atgttcaacc gacgtttcaa
    24361 gccaccacta agttctcctg gacgaaacga aacatatttt tctaatccca ctaattgaat
    24421 cacctcttca atcaaggctt tccttttctc tttttcggaa acatataaac cgcaaaaata
    24481 atcgatattt tcttgcacac ttagttcgtc aaagaccgca acgtcttgag gaacgacccc
    24541 aatcattgcc ttgacagcat aattttcagg agtcattgtt gtcccgaaca atttaatggt
    24601 tcctttgtca taatttaaca aagaaagcat acagttgatg gcagtcgatt tcccactgcc
    24661 gtttggtcct aacagaccta agacttctcc ttcttgtact tgcagatcaa aatgattcaa
    24721 tgctacaaaa tctccatagc gtttgactaa tttatcaatt tcgataatca tcatgaaaaa
    24781 ctccttccaa atttcttgtt gatctgacta taataaagtg caagaaaaga aactagtgac
    24841 gattggctaa accacacatg acatttgtca caatgaagga gtctgctatg cataaatctc
    24901 ggctactcaa cgaaactagc tttctactgg ttatctcatt gcttttgctg gtaagtcaag
    24961 gattcgttgc tatgcaagtt atctatttat tgatcgccgt tttattcact ggtgtcagtt
    25021 atttactgcc tacaaagcca aattggattt tccaagttat tctatttctt ttatgtgcct
    25081 ttttacctga tcttgtcctg tttctgccag cgacgatgcg agtgatcgcc cctgaagaaa
    25141 gccaccttga ttacttgttt ttgttaggtt ttttgttgat ttcgacggtt ggtaatcatc
    25201 cccccatcaa ggtcgccttt gtcgtaattt tgctgttttt ttccttttac ttacgaaaaa
    25261 aagatcaacg aacgaccgat cttgagaaaa agtacattgt ctccaaagat actcattggg
    25321 aaaaacaaca agagttatcg gaacaaaata aagcattggc cctgtcacaa gaaacacaat
    25381 tagccttgca aattgtggaa gaacggaacc gaattgcccg agacatccat gacaatgtcg
    25441 gtcatctgct gtcaagtgcg attttacaaa ttggtgcgct tgaaatgatc aatcaagaac
    25501 caaaactaaa agaaccatta gaaaatttaa gtgccaccgt tcacactggg atggatcgga
    25561 ttcgtcaaag tgtccatgac ctccatgaga catcgatctc ttttcaacac tccttagagt
    25621 ttctgatcgc tgattttcct tcttctgtta cgattgaagg aaacttgttt gaaggattga
    25681 atgaaacgca gcaaaactgc ttcttaatgg tcatcaaaga agccttaacc aacagtatca
    25741 aacatagtca tgccaaaacc gttgttttac attttcgaac gttgccggcc ttttatcgct
    25801 tgcaaatcac gaatgatggc accagcccac aaaaaaattc tgctcccggt attggtttga
    25861 aaacgatgcg tcaacgtgtt caagaacaaa agggacaagt ccatattttc caagaaaacc
    25921 agcaatttca gttaacgatc attttaccaa gggagggaca ccaatgataa cagtagtcat
    25981 tattgagatg atccatttgt gacgaaatcg ttacaaacca ttttagaaag cacgaaagag
    26041 atccgtgtgt taggcattgg tcattgtgcc aaggatgcgc tagtcttata cgaaacccat
    26101 cgtcccgatg ttttgctgac ggacatccgc atgcctaaac aaacgggcat cgatgctgcc
    26161 aaagaaattt tggctaaatt tcccaaagcc atccttttgc ttttgacgac attcaaagat
    26221 gaagaataca tccgcgaagc attttccatc ggtgtcaaag ggtatttaat caaacagaac
    26281 ttgcaagcga ttattccttc tgtcaaatcc gcttataatg gacaggccgt atttggtaat
    26341 gaaatcatcg agactttcac tcaaatgatg aagaataaac cgacgattga tagtcatgct
    26401 tatgcttctt ttagtgaacg tgagcttgca atcatcaaag aagtagctgc cgggaaaaac
    26461 aataaagaaa ttgcagatgc cttgtacttg agtgacggca ctgttcgcaa ttacatcagc
    26521 caattactag aaaagctaga tttacgggat cgcacgcaat tggctatcta tttttatcag
    26581 catttgcaat aagtcttatc taacacaaaa aaacctgaat gatgctcctt cgcatcattc
    26641 aggttttttt cacatcttct attcaattgt cccgcaaacc atttcctcac gattgaaaga
    26701 atcaaggatt cgcagggctg ccaggattcg ttggattgtt cggcgtgtta ggcggaaaaa
    26761 taggcgcctt gtctaacgtt ttttctagtt ttgattcttc acgatcgtct cgttctacat
    26821 caggactttc attcaatttg aattcttttt ccatctcatc taacttttta aagtcattgc
    26881 cagattcttt tgctaatttc gctaatttat cttcggtttc tttagccata aagtaacgcc
    26941 ccccttttga tttcagctta gcatatcaca ctgaaaaaac aaaagaaaac gctaaccaac
    27001 gatcattttt agctatttct catctttaaa atcaagcatt ttttgccggt ataaaatcgt
    27061 agggagaact gttttccata ccaatcatcg gatctactaa ctggttatcg atcacttctg
    27121 ctgtaagaac gaaatcttta gaaacagcag gttctacctc ttctttcttt tcttctccaa
    27181 caaatgaaga aaacagatct ggttccactg tttccattac ttggtttttc gtttcattcg
    27241 tcccactgtc atcttctaca ggtagctttt cttttacaac ccgtggctct tttgtatagt
    27301 tcccatcttc aaatggatca tttccgacca aagaatcttc ataagctgct agtttggtcc
    27361 ggagagtgat attcatttta tcattatcca tgatccttga tttcaacgta gtcatccggc
    27421 ttgctgattc attcgctaca caagtgacat aggcttgctc ttcccctaac aaaatcaaca
    27481 gttccttact gcgagtcact gccgtgtaga gcaaattacg ctgaagcatc cgctgatatt
    27541 gatggaccat gggcaaaata accatcttga attcactgcc ttgtgattta tggattgagc
    27601 aacaataaga aagagtgatt ttattccatt cgtttctttt ataagtgacc tcatttgcat
    27661 caaactggat aactaattca tccactttat cttcagaatc ttttgccaag atgatacccg
    27721 tgatttctcc catatctcca ttgaatacat tcaattcggg agtattgact aattggagga
    27781 ctttgtctcc tatccgataa acggtgtcgt tccaatgtac ttcctttttt ccaggtttcc
    27841 ctggattgaa tatctcctgc atcattttat ttaacgcatc gatccctgca ggtccccgat
    27901 acattggtgc taatacttgg atatcttgtg cacggaatcc cttatttttc gctttttcca
    27961 caattttaga gatcaagggt tcaatttgat aagcatcact tcggaaatag gaacgatctt
    28021 tttgattctt tgtgaaatct tttggcagac gtccttcttt gatctcatgg gcaagaggaa
    28081 taatactaga accatcacct tgccgataaa tctgattgag ttccattttg ggaatttgtt
    28141 cgatttccaa aagatcatgg agtacttgac caggaccgac agagggaagc tgatctttgt
    28201 ctcctactaa gataacttgc atattcgtcg gaatcgcttt gaataatgtg ttagccagcc
    28261 atgtatcgac catagacatc tcatcaacaa tcaaaagtcc gccttctaat tctttcgtac
    28321 tgactgaagg tgttttttcc cgtccattca gccccagaag acgatggatc gtactggcag
    28381 gcagacctgt cgtttcgttc attcgtttag ctgctcggcc agttggtgct gcaagtagaa
    28441 taggaaaaat ttcttgggta tattttgaag gatccaattc cagtccgttc aactctgcaa
    28501 aaagattgac gattccgttg atcacagtag ttttccccgt acctggtcct cctgttaaaa
    28561 taaatagagg ggaacggatt gcttcagcga tagcttgttc ttgcgaaggt ccatactgta
    28621 tgccgaatat tttttcgata cgccgaatat tcttctgcac ttctttttca ggataatgga
    28681 tttctttttt acgactgaga aggcgctgga tcgaattacc gatcccccat tcagaaaagt
    28741 acaaactgtt ttcgtaaaga ttagtctctt cttgttgtat ctttccttct tcaacaagtt
    28801 ggataattac agtagcaacc tgatcaggct ggatttcaat tgggcgactt gtttctaaaa
    28861 tacggatcgt ttgctgaagc aatgtctccg ccggtacgta tgtatctcct gtttgaactg
    28921 catgctgaaa aatctgatgc aagatagctg ctcgaattct tctcggtgaa tctgctgcaa
    28981 taccgatttg ttcggctaga ttatctgcac gtttgaaccc gattccttct atgtcttcga
    29041 ctagctggta cggattttct tcgatgatat ccaaagactc gttcttatag gcctggtaga
    29101 tactgaaagc taactgacta ccgaacccgt aacgattcag tcccatgatc acttgatcca
    29161 tcccgtggtt tagccgaatc gtctcagcaa gcatttctct ttttggcttc gtcaaaccgg
    29221 agatttgttt taataccgaa ggatcagcaa tgattttttc gatagcatct tctcccagca
    29281 attctacaat tttttctgct gttttcttcc cgattcctgg gaatttttcg ctggaaagat
    29341 acgctacgat cccatttgca gttgttggct tttcttgctc atatctttcg agtttaagct
    29401 gccttccgta tttaggatga tccactaatt ctccataaaa acgataagtc tcattttctt
    29461 gaatatcgcc gaaacttccg gtgactacaa tctctttttc acggtaatca gctgtggttt
    29521 cactgatgtg taccaaaagc actttgtaaa aattactagg attttgaaaa aaaatcgcgg
    29581 caactgtacc aacgaaataa ggcatttctt ctggctgcat cggaagccgc ctccttcttc
    29641 tatagtaaag gttctggtgc tgtttcagtt cctaaaaaat ctgtttggtt ccatactttc
    29701 agttcatatg gcaataatct gcaaggctct tcagtctcta aaatcgtcaa actactattg
    29761 aacaatccac ccatttcacg caactcactt agctcttttc ctgtcatcca ttgaatggct
    29821 gcagttaaag aagctccatg tcctacgaat aaaagtgggc catctttatg ttgggcaacc
    29881 gcatctgcta caacagtttc gatacgtgtg attgccgatt caatcgtttc tccgtgaaaa
    29941 ggagaaggat catacagatc taaatgatta cgcaaattcg gcagattatc cggatatttc
    30001 tggtacattt catcgatcga ctgaccttct aaagtaccca gccctagttc tcttagctta
    30061 tctgtataaa aaatctctac cggacgagca agctctttgt tgataccttc tgctgtatca
    30121 cgtgctcgct ttgctgtact ggaataaatt gctttaaaag gaacatattt tacctttttc
    30181 ccaaatgctt tgatttcttc gtaactttgt ggcaaaagcg gagaatcgcc attgctccct
    30241 tgaaaacgtc tttcctgatt ccattgtgtc ttcccatgtc gggtaaaata tagttccata
    30301 cgcagcaaac tcctgtcttt cttttatttt actctataat aaactgtcta atccagcttg
    30361 tcattttcat agtgatgcgt caattctaaa ttagggaatc cttgctggcg aagtgcttca
    30421 tacactatca ttgcagctgt attcgataaa ttcaatgagc ggacatgctc atcattcata
    30481 gggatgcgta aacacttttc ttcgttttca cgcataaatg tttctggcaa accggttgtt
    30541 tcttttccaa ataaaaagaa gtgctcttct ccatcagcaa aattttcgtc actatatact
    30601 ttatgggcaa attttgagat caaatgtagt ttacgattac ctaaaaatgt taaaaaagct
    30661 tctaaattac tgtggtaagt aatatttaca tcattccaat aatccaaccc tgcccgtttt
    30721 agatgtttgt catcagttga aaaacctagt ggctcgatta aatgaagcgg agtattcgtg
    30781 gctgcacacg tacgggcaat atttccagtg tttgcaggga ttaatggttc aaataaaacg
    30841 atatgattca tcaggatcat tcctctcata tttatgttcg tttctatttt ctgacaattt
    30901 gatttttgca taaaaaaaaa cgtatcaact cggtgtcagt cactgcgagt cgttacgtta
    30961 gtgaagctct tctcactaac ttttatcaga acaggaacct gataaaaacc aatgaaaaaa
    31021 caacttgttg ctaatataac accctcaact tttttttgca acctctactc attaaaaact
    31081 gctagaaaaa cgttttctct tagttccttt taataattct atgtcaattg taatcttctt
    31141 caaaggattc ttatataagt ctgctttgat tcgctcttct acctgccatt ctaaaggtga
    31201 catggccaaa agatcttttt gattttcctc tggtatctca aattcatagg ttacacgctg
    31261 acgttcagtt aatttcattg cttcagcaaa cttggctact acacgttcat tggaataact
    31321 ctgtttttca ggttgattcg gataaaaagc ctgacgtagt tcctttaaat aattttcttg
    31381 tggcaccacc ttcaacactt ctccatcttc tgagagtaca cgttggaatt cttggtaatg
    31441 agaaggcgaa aagatattta aaacctcgtt gaatgtttga tcagcaaacg gcaaattggt
    31501 caaatcagct acacaccaaa atgcttcggc agattgttct gtagctgcgt agactccttc
    31561 ttttgaaata tcaaatccaa cagcattttg aatctttttc aattttgaaa gttcattcaa
    31621 gaagctacct tctccgcagc caacatctaa tagcgagact tcttcaggta aatgctgcgc
    31681 aatagtctca ataacgggct gatacatgcc agattggatc attctacgcc ttggaacgaa
    31741 cattccttga tcgtattctg ttttaaccgc atgggataaa aaataaagtg ttcctttttt
    31801 ggaaaggtca aaacggtgat tatttttgca tatcaagcca ttatcagact tttttacttc
    31861 ttcttggcaa accggacagc ggaacagctg atgattttct gccaaaaact ggcgtcctcg
    31921 gtcaattttt tttaacatag ttccccctca tttctgtttt atcttaccgt tagatgctca
    31981 ctttggcaac tgctaaaaat atgatacaat gcctagaaga aagtgaaaga taaaggagaa
    32041 gattagatga tcaaagaatt ttgcgctgaa aactacacat ctattcctct agcaattgct
    32101 agaggagccg atcggatcga attgtgtgac aacttagcgg tcggtggcac aaccccgagt
    32161 actggtgtga tcgaagaaac gttaagttat gctggggaaa aagatatccc cgtaatgacg
    32221 attattcgtc ctagaggggg cgacttcgtc tataacgata tcgaattgaa aatcatgcat
    32281 actgatttga ttgaagcaaa gaaattaggg acggatggcg tagtatttgg ttgtcttact
    32341 ccttcaggct ggctagacga agaggcctta gaactattga tcgaaacagc agaagggttg
    32401 cagatcacct tccatatggc ttttgacagt attcctgaag atcggcaatt cgaagcaatc
    32461 gattggctgg tagaacatgg tgttgaccgt atcttgactc atggcggtcc tgctgacaaa
    32521 actattgtag aaaatttccc acggcttaaa gaattgattg cctatacaga tggccgctta
    32581 atcattttgc ctggtggtgg tgtcactgca gagaatgcag cacatgtagc taaagaattg
    32641 aacgtaagcg aagtacacgg aaccaaaatc gtggatcttc agttataatc ttataaacaa
    32701 aaaaacattt gcaacaggca taatgcttat tgcaaatgtt tttgtttttt tacataaaat
    32761 agcccataaa taaagaagca atattgaaat aaatcagtac actcgtcaaa tcgctcaaag
    32821 tcgtgataaa gggaccgctt gctactgctg gatcaaatcc tagacgatcc attagcatcg
    32881 ggattaaact acctgcaaga ttcgccactg tgattgcaaa tagcatagcc atccctatga
    32941 caaaccccaa gataaaatta tgtttccaaa aacctacgat tagaaaaatc gtgatccccg
    33001 taacagctcc tgtgacaacg ccggtcaaga cttcagccaa aacaagtcga acaaatccat
    33061 gatctttctc atcagaaatc gcaagtcgac gaacagccac agctagcgac tgggttccag
    33121 cattaccggc agttcctgta atcagcgaaa tgaagactgc taaaatactc gcttcactta
    33181 ccaagtcttc atagtgactg attaaagaag cggtcgacat acctaaaaac aacagagtga
    33241 tcagccaagg caaacgtcga gacgctgcct ttactggatt ttcattaatt tcttccacat
    33301 tgaccccggc aagtcctgaa taatcgctag cagcttcatc atcaataacg tcgatgatat
    33361 catcgactgt gacgattcct aacaaatgat catcataatc ggtaacagga agagctaaga
    33421 agtcataatc tctaaatgtt tgtgccacat cttcttggtc gtcgcctaca tgaactgaaa
    33481 gcactcgttc gctcatcaga tcagaaatca ttgcgtcgtc atcattcacg attaggtcgc
    33541 gcagagaaat aacccctact agctgacctt cttgattaac gacatagatg taatagattg
    33601 tttcagctac atctgcttcg ttcttcagca cgtacatcgc agagcgaaca gtttgattgg
    33661 ctacgatcga aacaaattca gttgtcatga tcgcgccagc cgtttcatcc tcgtaatgga
    33721 gaagttcctt gatttcactg gcatcgtctg ccgacatcaa acttaaatat ttagcaattt
    33781 gctttttatc cagcgtatta agcaagtcaa cagcattatc tgtatacatc tcagaaagca
    33841 tatcagctgc atagctagga cgcatttcat ctaggtattc tttcatatgt tcgttgtctt
    33901 cttcaatcac atcgaacata tccgctaatt ctttaggcga cagataggaa tagatcagct
    33961 gcctgttctc ttctgtaaga gattggtaga attgcccctg ctcatagata tgcatctcta
    34021 aaaaactgtt tctgaatgcc tgaatattat tttcccttaa atcttgagtc aactgggcaa
    34081 atctttcctc taattcttga ttttcatcca aaccaatctc acctcgcctt attcttcaaa
    34141 ccgtctcaca actgcctcca tgtctgcggc taaaggctgc ttgaactcca gcaactcttc
    34201 tgtaaatgga tggtaaaaac gcagatcata acaatgaagg gcttgtcttt ttattcccag
    34261 atccattcgc ccgccataca tatcatcacc gagcagggca cagccaattg cggaaaaatg
    34321 aactcttatt tgatgtgtcc ttcctgtatg cagctggata tcaactaacg cgatttctgt
    34381 tagtcgcttc tctatccagt actcagtctg tgccgaacgt ccttcatcag agaccattcg
    34441 cttcaatagc gaagaataat ctcgggcgat cggcaaatcg atatgtccat gtgccgataa
    34501 atctgaaagc cttccactga ctaaggcctg atattttttc acaaacttct tttcacgaag
    34561 ttgcatatct aattttgcgt gcgcaaatcc atgctttgca aaaagcatca aaccagatgt
    34621 atcccgatct agtcgcgtca ccacatggat tacttggttt tcgtaacctt gttttttata
    34681 ataagccttt acacgattag ccattgtccc atttggatgg tattgggcag gaatagacga
    34741 aacgcctgct ggtttattta ctaccagaac gtgttcatct tcatacacga tatcgatcgg
    34801 tgtttcatcc aaaagtacgg tttcatgttc tccttcagca ggaatcacaa tggttacctt
    34861 atcatcctta gctagagaaa acaagacatt ttctacttga tcattaactt tgatttgacc
    34921 gccttgaaat tttattttcg ctaacagacc ctttgagatc cctttttctt ttaaaaaata
    34981 ttttacttgc tgagaatgat ctttctcata atcccatgta aactgcatat caatcctcac
    35041 taataaaggc atctttcaca cgatgccaaa aatgcatatg tcgataagaa gcaaaatgga
    35101 tccgttcttc tgctattcga taataaattg agcggatatt tgcttttgcg acattcaatt
    35161 gatcaaccgt tacaagataa tcttctgtat tttccaattt tatttctagc cattcatcat
    35221 gggcaatcac gatcggagaa cccaaagtac gaaacactcg attattcagt gaagcaatct
    35281 ctgccaactg aaaggcattg atactaggat ggatcacagc accgccgaca cttttattat
    35341 atgctgtcga accagtaggc gtagaaatcg tcaatccatc tcctctaaaa ctttcaaaca
    35401 gctcgttttt gatgtataca tcggctacca tcgtacgatt ggcccgttta atcgttgatt
    35461 cattcagtgc taaaaaatgc tggtccggtg tttcatccaa ataactgatc cgaacatcca
    35521 gcaagggata actaacactt tgttcccggt tcgttcgtaa gctgtctact agttcttcca
    35581 gctcgtaatc ccgccaatcc gtgtaaaacc ctaaatgacc tgtatgcacg cccaagaaac
    35641 gcacatcatt cagacaatga ctgtagtgat ggaacgccga caataatgtg ccgtctccgc
    35701 cgactgagat gaccaattct ggctcatgat catctcgttg aatccctgct tgttctaaaa
    35761 gtgcgttcaa acgttctgtc acttctatag tctttgcttc tttattatga acgatcgcta
    35821 ctttcatccc ctgcctcctc tctttcgctt tctttcttca aaagcgttcg atagtagaca
    35881 tcatctgatt ttcctcttcc atgggagaat aaccgctgtg cttcttggat ttcttcacgg
    35941 atactggaca tctcttcatc caactgatag gcagcttctg ccgcacgctg aagtcgttca
    36001 ctcatttctt ctggaaaaac gccttgatat ttataattca gcgaatgctc aatcgttgcc
    36061 cagaaattca tcgcaagagt ccgaatctgg atttccgcca aaattttctt ctcaccgctg
    36121 atcagctgta caggatactc gatcaccaga tgataagaac ggtagccact tgctttttta
    36181 tttgtgatgt aatctcgttc ttggagaact ttcatgtctt ttcttttacg gatgatctca
    36241 acaacttgat ggatatcttc tacaaactga cacatgatcc ttaacccggc aatatcagac
    36301 atttcttctt ctaaacgtcc cattggaata tgccgtaatt gtgattttgt tacaatacta
    36361 tcgactggct tgactctccc agtgacaaat tcaatcggga catgtcgatt ttgttcacgg
    36421 aactgtttgc ggatcccccg cagtttcact ttcaattctg atacagtttg ttcatatggt
    36481 gctaaaaatg cttcccaatc tcgttccata gctgatacct ctacttcatt aacattcatt
    36541 gtttattcta ccatatatgc aaagtttttt cgtactcttg atttatgcat tgaccaaaaa
    36601 aagaaaatta ggcaaaatag acaaaaagga gtcgatcaaa tgacacaaat gcttgaaatc
    36661 gaatataaat ctatgctgac aaagcaagag tacgagatat tcatcgctca ctatcaatta
    36721 acagataaag atttccgtgt acagactaat atttatttcg ataccgttga tgaacagctg
    36781 aagaaacaaa attgtggcct acggatccgt ttcgttggtc atcaagctga atatacatta
    36841 aaaacacctg ctgaaaaagg gcgtctcgaa acaacggata cctttataac agaagaagca
    36901 gaagcattta tcttcgaaaa acgtctgcct cgttcaggcg cagtttttca aaaactttct
    36961 gatttgaata tcgatcctgc ttcattaatc caaaccggaa agcttacaac aaaacgggct
    37021 gaattcccta ttgaagaagg acttttagct attgatgaaa gctggggaga caatctacac
    37081 gattttgaac tggaactaga ggtaggggat gcatctgtgg gaaaaattgc ttttataaat
    37141 tttttaaaac gtttttcttt accttatcgt cctgctaaga acaagatcca acgtatgctt
    37201 gaagctaaaa attagaaacc tttcattttc acaaggcact tgagttcatt tttttacttt
    37261 tttctgttaa gttataggat gtgaaagaat ctagtcacta gttgtataga atctattttg
    37321 ggggagattc atatgatcga aatctattta tttgttaatc ctttagggga atactgtttc
    37381 gaaatggaac aacagttatt acaatttatc gaagaagagt acgggacacc atcaaaagaa
    37441 aagatgcaat ttcgctttct tcctttggtc aatttgcaga caattgggga tgtcatgcaa
    37501 caaaaaggaa tttctcagaa caatcttgag gaacgtaatc acttattctc aatgacttat
    37561 tctgcagcat tggactgcaa agcggcgcag tttcaaggaa agaaaaaagg ccgccgtttt
    37621 ttgatcaaat tgcaagaagc agtcggctgt aatgacatac catattctca agaacttgct
    37681 gaatccatat ttgaagaaat cggcggcgat ttgaatatgt ttaaagaaga tcgtcagtct
    37741 gacttcgtta aagaagtatt cttttctgat caaaaaattg ctcgggaaat gggcatcaca
    37801 aaacaccctt ctgcagtcgt ttacaattat gcctgcgacc gtgattttgg tgttttagta
    37861 gaagtaaaag gtacaagtag cctttggaga atcacagagc tgtgtaaaac aacagataac
    37921 tcatatcaag tttttaatgg tgaacattta catacaaatg attctcagtt ccattcaaga
    37981 actagccatt tacacttgct ttctaattaa aaaaatacgt cgactccctt acaagagagc
    38041 cgacgtattt ttttcgtttc tattcgagaa aatcaaaaat agaaagcctt ataaacgatc
    38101 gatcaattct tctaattcat ttagacgttg ttcaaacatc gacattgcat cttcaatata
    38161 tgcagcctgt gtcatatcaa ctcctgcttt tttcattact tccacaggat aatcgctgtt
    38221 acctgctttt aggtaagcca agtagttctc tagtgcctct ggttcctggt ttagtatttt
    38281 cttcgccagt gctgaagcag cagaaaagcc agtcgagtat tggaaaacat agtaattata
    38341 ataaaaatgc ggtatccgtg accattcgaa tttaatttct gggtcttctt cgactgctgg
    38401 accataatat tttgcattca atttaccata actatcactt aggtattcac tagtcaatgg
    38461 cacacctttt tcatcttccg tatgcatgaa atgctcgaat tcagcgaatt gtgtctggcg
    38521 gaaaactgtt cctttaaacc catccaagta atggttgagt acataagccc gtacacgagg
    38581 gtctttttct gtttccaata aatactccgt taggatattt tcattcgttg tcgaagcaat
    38641 ttctgctaaa aagatggagt agtcgccgta cacataaggc tggttcgaac gagtgaaata
    38701 actatgaaca ctatgtccca tttcgtggac aagcgtaaat agctgatcca gcgtatcatg
    38761 ccaattcaat aaaatatatg gatttgtgtc atagcttccc gaagaataag catcgcttcg
    38821 tttcccttta ttttcgacaa catcgatcca acgttcagag aatgcttttt ctacgatggc
    38881 catgtattct tcacccattg gtttcagtgc ttctaaagct ttttcttttg cttcttcgta
    38941 cgtaaaggta attggagctt cccctaagac cggtgtataa agatcataca tgtgcagttt
    39001 ttccacttct aataaacgtt tccgtaattc catgtatcga tgtaacaaag gcaaatgctt
    39061 gtttaccacg tctaccaaag tatcgtatac actttcagga atatgattat tgctcaaaga
    39121 cgcttctctg gcagagctgt aattacggac tttcgcttta aaattatgtc cttttatatg
    39181 tgtgcctaaa gtagaagcaa atgtatttct aaattgttcg taaacactgt acaatccctt
    39241 aaatgctgct tcgcgcaccc ttcgatccgt gctttctagc aactgaccat acacgccatg
    39301 agataattgg actatttcac cattttctcc ttcaatcgtt ggaaaaacta gatctgcatt
    39361 attcaaaaca gcaaatgtat cacttgaagc atcaaagatt tcacctgctc cagcaagaag
    39421 agattcctgc tcagcagata agacatgggc tcgattatct actatttgct ggatataatg
    39481 gcgatagact tccaattttg gttcttcttt gaaatattgc caaatctggt catctgacaa
    39541 ttgcaatatt tctggttcaa accaggaaac ggcttcacta actttcgaaa acagactgct
    39601 tgctcttgca taaagcgctt ggtaatctgt atttccagtg tcttgatcgt ttttaaggtg
    39661 cgcatataca taaatgactt cagtttggcg ataaaccctc aatacgaatt caatcgcatt
    39721 taaaaattga gaagcgcctt gatcaagtgt tcctttgtgt ttttcagatt gttttaactc
    39781 ttctgataat tccaaatatt tctcatcaaa ctcttggtcg cttgaaaaga tcttggtcaa
    39841 gtcccaagtt aaattttcag gcaattcttc tcgttttggc aactgcttta cttccatgaa
    39901 atccactcct tttcttccct tagctctatc tatcaaaata gatcatagtc taacatccac
    39961 atgtcacagc atctaacttt tctactcatt catgtgtctg cttccatcaa ttttagcata
    40021 actaaatctt gttttacata gaaaaccgat tcctagccta tttttatact tactattttt
    40081 tcaaagaaga caagtgtatg cattcgttaa aaaactgctg atagaccatc tcttcatcaa
    40141 tcataggaaa aagccattcc cttttatgtt cttttatttg gcaaagccat gagttgaaat
    40201 caggaacttt ctggttttcc aaggcttctt cgtacaacat tcgataaaga aaaacctgtt
    40261 cttgtaaata aaagaaaaac cgactttttt gatagatcca cgatgataga tgcaaaagat
    40321 gtttatgacg caagtagcat tgttcttgta tggatactgt attcttttgc ttgtgaataa
    40381 gttggcgtgt cagccatttc ttcccatctt cactcacttt ttctattttt ttcatttggt
    40441 tcattttaat ttcgttacta gtgaatattt ctttaaggtc cctactagaa aaagtccagc
    40501 agctactgca aaaggaaata tgccctgata cagtctctgt aatatgatga tacaaataaa
    40561 gcttttgttt cttgagatca gctttccagc aatgcactcc tctttttcta ttatagtagc
    40621 agcaactttt ttctatcaat gagaattgcg attgtccaag aaaagaatta ccaagtatcc
    40681 accatacttg gtaactgtta ttcaaataat tttgggttct ttcaataaat ctctggtgag
    40741 acagtctaga acattggatt tctattgcta gattcgccaa caaaatatcc ggcctttgcc
    40801 gcaacctcgg caaataggct tccaaaaata ctggttctac tgactgtccc agccaatcca
    40861 tgaagcattc ctttaactgt aagtgctctt ttgattctct ttcactaaag accgaacagt
    40921 tatctgattt ttgatgggca aaatgagcac attttttctt tccctttttc aaaatcactc
    40981 tttttcgaca gccacgacaa taatatttct ttcctttttc tgcatgttta gcaaaacagc
    41041 acttattata ttcattcatt gcaagcagca tttttctccc tctgaactta tttacgtcta
    41101 agaatttatt tacacgaaaa aagagggcgt gaaaaagcta ttctgcatca agtaataaga
    41161 acagctaaac aaaaatagtt cccaatattt ttcgtttggt tgggcttgtt accgcagatg
    41221 caagcttttg aacacattta tacgaaaaaa agactgcgac acaatttttg tcacagtctc
    41281 ttactataat gctgatttat acaaaataaa atttcgttcg ctcgatagca ttacgcgcca
    41341 tcagacattg tccatattca ttcaatgttt ctggtgtacg atcgctcttt tcagcaaact
    41401 ccaaaattcg agcaatctga ttctctacat ccgcttggtt gagattttcc atccagaagt
    41461 gaacgactag gtaatagcgc tcttcgaata aatacaaatc agtccaagca gactctagtt
    41521 cgacatctgt tgcaagttgg atcacttgtt caaaatcttc aaaagcaaaa gttgcttgtc
    41581 tttcctgttc ttctagggcg cttccagata attcttcctc cggatcgctg ccaataatct
    41641 gctgacgaag aaagtcactc acatcttctg aatcaacagt agaatcttct gattgagaaa
    41701 tttcttcatc cggaagattt ttactgataa acagttctaa tccgtctcct tttggcaaaa
    41761 cttggaaagt gacagcttcg cttcctcgga actcctcttc gatatcaact tcttccaaaa
    41821 tactataaaa gaaattttcg atttctttgt gattgccaag taaatccaaa aatgaaacgc
    41881 cccgttcctc taaatcttca tgaccaatta gtacacggat cgtattttca ttaatatgtt
    41941 ccatttccat tttgctacac ctcactttgc cacaatgttt aatatagatt cattgtagcg
    42001 aaatcagctt aaatgtaaag aaaaaaaatc tcgctgttaa gcgaatttaa taaaaccagc
    42061 ggatttttca acctttcaag ctatcttatt taactataac atttttggca gacgaatact
    42121 atatagccga cttttcagct aattttccct ctctattttt cgtgcaatca tcatcttcta
    42181 ctaccttttt atacatctaa tcaagaatac tttttgtaaa aacaaaaaaa cttggaaaaa
    42241 ctatctttgt aatagt
//
LOCUS       DAJSKS010000007        40431 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJSKS010000007 DAJSKS010000000
VERSION     DAJSKS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.007"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(54..788)
                     /locus_tag="ON396_000360"
     CDS             complement(54..788)
                     /locus_tag="ON396_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCR3112494.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="ON396_000361"
     CDS             1115..1525
                     /locus_tag="ON396_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR3112495.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="ON396_000362"
     CDS             1518..2219
                     /locus_tag="ON396_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCR3112496.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="ON396_000363"
     CDS             2219..3805
                     /locus_tag="ON396_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3112497.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="ON396_000364"
     CDS             3824..4045
                     /locus_tag="ON396_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCR3112498.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="ON396_000365"
     CDS             4042..4803
                     /locus_tag="ON396_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HCR3112499.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="ON396_000366"
     CDS             5183..5692
                     /locus_tag="ON396_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCR3112500.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="ON396_000367"
     CDS             complement(5767..6306)
                     /locus_tag="ON396_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCR3112501.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ON396_000368"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="ON396_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR3112502.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="ON396_000369"
     CDS             7319..8170
                     /locus_tag="ON396_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR3112503.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="ON396_000370"
     CDS             8198..8833
                     /locus_tag="ON396_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HCR3112504.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="ON396_000371"
     CDS             8853..9626
                     /locus_tag="ON396_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3112505.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="ON396_000372"
     CDS             10034..10831
                     /locus_tag="ON396_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCR3112506.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="ON396_000373"
     CDS             10809..11222
                     /locus_tag="ON396_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR3112507.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="ON396_000374"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="ON396_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR3112508.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="ON396_000375"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="ON396_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR3112509.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /gene="maf"
                     /locus_tag="ON396_000376"
     CDS             15999..16559
                     /gene="maf"
                     /locus_tag="ON396_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HCR3112510.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ON396_000377"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="ON396_000377"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCR3112511.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="ON396_000378"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="ON396_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCR3112512.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="ON396_000379"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="ON396_000379"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR3112513.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="ON396_000380"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="ON396_000380"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR3112514.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="ON396_000381"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="ON396_000381"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR3112515.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="ON396_000382"
     CDS             21906..22484
                     /locus_tag="ON396_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3112516.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="ON396_000383"
     CDS             22536..23018
                     /locus_tag="ON396_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCR3112517.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="ON396_000384"
     CDS             23135..23509
                     /locus_tag="ON396_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112518.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            23939..25117
                     /locus_tag="ON396_000385"
     CDS             23939..25117
                     /locus_tag="ON396_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HCR3112519.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIE
                     SFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE
                     "
     gene            complement(25207..25473)
                     /locus_tag="ON396_000386"
     CDS             complement(25207..25473)
                     /locus_tag="ON396_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112520.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(25466..25789)
                     /locus_tag="ON396_000387"
     CDS             complement(25466..25789)
                     /locus_tag="ON396_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112521.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            complement(25801..27057)
                     /locus_tag="ON396_000388"
     CDS             complement(25801..27057)
                     /locus_tag="ON396_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HCR3112522.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            complement(27054..27449)
                     /locus_tag="ON396_000389"
     CDS             complement(27054..27449)
                     /locus_tag="ON396_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112523.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            complement(27492..27743)
                     /locus_tag="ON396_000390"
     CDS             complement(27492..27743)
                     /locus_tag="ON396_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112524.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            complement(27743..28138)
                     /locus_tag="ON396_000391"
     CDS             complement(27743..28138)
                     /locus_tag="ON396_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112525.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            complement(28493..29494)
                     /gene="ccpA"
                     /locus_tag="ON396_000392"
     CDS             complement(28493..29494)
                     /gene="ccpA"
                     /locus_tag="ON396_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCR3112526.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            29704..30807
                     /locus_tag="ON396_000393"
     CDS             29704..30807
                     /locus_tag="ON396_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCR3112527.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            complement(30870..31472)
                     /locus_tag="ON396_000394"
     CDS             complement(30870..31472)
                     /locus_tag="ON396_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR3112528.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(31475..31915)
                     /locus_tag="ON396_000395"
     CDS             complement(31475..31915)
                     /locus_tag="ON396_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCR3112529.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            complement(32040..32978)
                     /gene="galU"
                     /locus_tag="ON396_000396"
     CDS             complement(32040..32978)
                     /gene="galU"
                     /locus_tag="ON396_000396"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR3112530.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            complement(32993..34018)
                     /locus_tag="ON396_000397"
     CDS             complement(32993..34018)
                     /locus_tag="ON396_000397"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR3112531.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            complement(34062..34892)
                     /locus_tag="ON396_000398"
     CDS             complement(34062..34892)
                     /locus_tag="ON396_000398"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR3112532.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            complement(34907..35845)
                     /locus_tag="ON396_000399"
     CDS             complement(34907..35845)
                     /locus_tag="ON396_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCR3112533.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            complement(36012..36362)
                     /locus_tag="ON396_000400"
     CDS             complement(36012..36362)
                     /locus_tag="ON396_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR3112534.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            complement(36362..36682)
                     /locus_tag="ON396_000401"
     CDS             complement(36362..36682)
                     /locus_tag="ON396_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HCR3112535.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            complement(36979..38547)
                     /gene="liaX"
                     /locus_tag="ON396_000402"
     CDS             complement(36979..38547)
                     /gene="liaX"
                     /locus_tag="ON396_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HCR3112536.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            complement(38647..39414)
                     /locus_tag="ON396_000403"
     CDS             complement(38647..39414)
                     /locus_tag="ON396_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCR3112537.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            complement(39411..40184)
                     /locus_tag="ON396_000404"
     CDS             complement(39411..40184)
                     /locus_tag="ON396_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCR3112538.1"
                     /translation="MHGDEWQLVAKKNDGWLFFFTSIFIGAMLLIGGKQGLFALGSLI
                     INIVLLLLLLLIFTKTEGISLLNKSILFVVIGMIISILALDGWNRSSIIKISAAVTSV
                     FLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATL
                     EELTKRQPEITAKQLWLSGRQVGRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKE
                     TIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTVRRLKR"
BASE COUNT        12492 a         7758 c         7915 g        12266 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgac
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcaccgggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatggaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaaca atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt tgagagtata aaatattttg tgtaaatgaa aaaatccata caaaaaagga
    23881 agtcccttct gtagaataaa gttaacgaca accaattcac agaaaagagg acttccctat
    23941 gaatgatttt actacagaaa ttgtgcaaac tctagtcact aaaggcgatt taaatgaatt
    24001 attccgttcg cacttagaaa aagcgataaa cacactccta cggactgaat taacggcttt
    24061 tttagattac gaaaaatatg atcgcactgg ttttaattca ggtaattcga gaaacggttc
    24121 ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca ttggaaatac ctagagatcg
    24181 taatggtgag tttaaacaac aaactttacc agcctacaaa agaacaaacg atacattgga
    24241 aaccactatt atccatttat tcgaaaaagg tgttacgatg tctgaaattg ctgatttgat
    24301 cgaaaaaatg tacggtcatc actatactcc acaaaccatg tccaacatga ctaaagttct
    24361 gactgaagaa gtaaatgcct ttaaatccag agccttaaat gataagtatg tcgctatttt
    24421 tatggacgct acttacattc cactaaaacg tcaaaccgta tccaaagaag cgatttatat
    24481 tgccattggt atacgagaag acggcactaa agaagtactg agttatgcga ttgctccaac
    24541 tgaatcaaca tacgtttgga atgagctgct acaggatatt aactccagag gagttcaaga
    24601 agtcttgctt tttattacgg acggcttaaa aggcatgaaa gatactatcc atcaaattta
    24661 tcctaaagca aaatatcagc attgttgtat ccatgtatct cgtaacatcg ctcataaagt
    24721 acgtgtcaaa gaccgaaaag aaatctgtga tgactttaag gctgtttatc aagctaactc
    24781 aaaagaagaa gcgaatacct tcttatccgg catgattgag aaatggaaga aaaactatcc
    24841 taaagtgacg cagtcactca tagaaaacca agacttatta actttttatg attttccacc
    24901 tagcattcgt agaaccattt actcaaccaa tctaatcgag tctttcaata agcaaattaa
    24961 aagatacagc cgtagaaaag agcagtttca aaatgaagaa tcactagaac gctttctagt
    25021 cagcattttt gatacataca atcaaaaatt tctaaacaga agccataaag gttttcaaca
    25081 ggtaaccgat acattagttt caatgtttac tgagtaacta attattttgc aggaggacaa
    25141 tttatttaca caaaattatt gacgctccca aaaaccattt tcacaaaatt tttctatata
    25201 actttactaa cgaaactgtt ttaatttctt ttcttgtctt tggaataatt cagttagttt
    25261 ttctgcaaat tgccctttta ctgatccttt caagtgtaca tgtcggttag aaaattcttg
    25321 agcagcttct tctgcagtca tttggtagtt ttgatctgtc aatcgttttt ctgcccaaat
    25381 ttgctgagat atccccatac caactgaacg tacatttgat cgatgtagat ttttctctaa
    25441 ttcttttagt aattgcatta aaaattcatt cataagatga tttctccata gttatttgtc
    25501 tgattttttg acgataggaa cgttctagtt catcttgctg ctgatagagt tttcgtcgtt
    25561 ctacttgtag ggcttgttga taatgctcct gataattcga ataacttcgt cttgcttgaa
    25621 acagaggctc attatctaaa gctaagttct ttaatacatg atgcagtcgc tgctcctctt
    25681 gctccatcaa tcgtaatcct tgattcttaa agtggatcaa ttcatcttct tgagcttcta
    25741 attgtcttcg ttcacttcga taatctcttt ccaattcttc tagtttcatc tacttctcct
    25801 ttaattgaat tgctgtgcta attctcgatc tcgtgccacg atttcattga ttttgtcttg
    25861 gatctccttc gccaatgtat caaaggcctc ccccatacgt cttgccttat tgattcgtag
    25921 cagatagact tctttcgcag aatcgactaa gttttctttt gtggcgccca cactagctaa
    25981 tgcttctatg atttcctgtt cagtcaacga aggggctaca acacgtgctt gccgcaagcc
    26041 ttcttgccat tttttctcta gctcaaaaat tcctctttga ttgatgcgga taacttcttc
    26101 catagcgctt ttcatttttg ccgataactt ttccactact aacctagctt gtgtgtcatc
    26161 taagtaaatt tgttcattaa ctgtcagccg gccgccactt aatcgtagtt ttttacgtat
    26221 gttgttcagt tcttgcaact gataggaaaa caactgggtc gcttggtttt ctttctgttg
    26281 ttttcgagct ttaacattgt tttcattttc aggaataatc agctttccat tttcatcaaa
    26341 cttccaagag gttaaactgt gggaagcaag cagattcaag aatgctgttt ccatactgat
    26401 ccgaacttca gcacctgtgc cattaccacc aaaattaccg ataaaatcgg cgtgatttcg
    26461 gtaattggtg agcataccgg gattttcttc cacccatttt tttgcttctg gtgataagag
    26521 atcatacggg tccggcccgt tgaagccgac atttttccat tgtttctctg ctgctacata
    26581 taatgctaag aatccaccta atgaatggcc ggtagtagaa atagcagctt ctccatattc
    26641 cttttttact tcattagcaa atgcaatcgc tgaattgatt tgccccgctg ttttcaatcc
    26701 actaacggat aaaatcttgt ttgttgttgc tttttcagaa aaacttgctc ctggtctatt
    26761 cccgtagaga ttcatgatac ttcttgcatc cgtttccaaa tctttccaat cacttaagtt
    26821 cgttcctgca aaagcaatca ctacttgaga aagatccact ttaccgttct tgattggcgc
    26881 tacagacatc gcctgcatgc cgtccaatga gtctttgatt tgtaggattt gaaattccat
    26941 accagcaaat tctttaacag agttcttttt gtatccctca ttgtagcggg gatatttggg
    27001 atctagccaa taaaccttct cactaatatc attgtaatgc ttatcgttta ttgtcatact
    27061 aactcctcct gtccgttaga aaaaattact tttactcttt ttatagtgac tcctgtcttt
    27121 tgtaccgctc tgttctcaat gccaaaacca ccaagttcat ttctctcctt ccaaaaacta
    27181 taatcaaaat aaacagattt cccttcatta tttgtcattg tcacaagtgc accataactt
    27241 ccggtaacag ggtttttttt aaattcttct accttcactt ccctgatatc cgcaaaggta
    27301 tttttcagaa ctttgactga ctgtttttct actgcgagta aatctttctg cctttgtgct
    27361 tctcttttgt ccatatagac tttccctccg atccctaata tgacgattaa taaaatggct
    27421 aagctgagtt ttcttatttt ccttgacaag aacgtgcctc ctttcttttt atgtaagtat
    27481 tgacaccttc attatttaag tacgcgaaag tttgcaggaa tttttttatt tttgaaattt
    27541 ttctccaatt tgctatggac gattggcgct acagccatcg cctgcatgcc gtcgaaggag
    27601 tttttgattt ttaggatttt atacatagtg ccgttaattt taaaacttaa attttcttgc
    27661 atttcaggta catagtctct atgtttagga tctagccagt aaacagcatc acttaattca
    27721 ctatattgtt tgtcagtaat cattaaatac tttcctcatt tccattagag taagttactt
    27781 taactttatt gagcgtattt ccttcttttt gctttttttc atcaactatt ccgtaacttc
    27841 ctatctcatt tctttctttc caaaaactat aagaaaaata aatcgattga tcttgtttat
    27901 tagtcattag aataactatc cggtatgaac ccgtcatttc atttcgagca gatttttcaa
    27961 tcttcacttc cttgatatcc gcaaaggtat ttttcagaac tttgacggac tgtttttcta
    28021 ctgcgagtaa atctttctgt ctttgcattt ctcttttgtc catgtaaaat ttccctccga
    28081 ttcctatagt aagaatggtc aaaatgatga ctatggtctt tcgtcttttc ttaggcaaat
    28141 agaagtctcc tttcgggtta tatatgatgg attgtttctc ctttaagata agtacgcaat
    28201 cgagcctgga tttttttgga ttttttagag ttacttatct acttttctac tttctttttt
    28261 atcacgtttt cattttaaag taattttgat agtagtgaac aaaatgtagc atctacgcac
    28321 agattgtgtc gtatacgatg gacgtaaggt ttgtttccgc cattttctct ttttttaaag
    28381 tcacaaaaaa aaaggggtta tgacaaaatt ttgtcataac cccctttctc gtataaacgt
    28441 tgttccagaa gttgaccact tttggaacaa cctttaaatg attgatcttt tattatttag
    28501 ttgatccttt ttcttcgatt ccataaggca agataatcgt tttttcttcg acttcttctt
    28561 tattcatcat tttcgtcaat aggcgcatgg ataccgctcc taaatcgtat aatggttgtg
    28621 tgatacttgt caaacgagga cgagctactt ctgtcagtaa agaattgttg cttgtgatga
    28681 tttcgaattc ttctggtact tttacgccgc ggtctaataa accatccaag ataccaatgg
    28741 ctaattcatc gtctgaaaca tagacagctg ttgcaccgct attatggatc cggtcaacta
    28801 gagacagacc atctttaaag ttgtattgtg cctcaaaaat caatccttcg ctatatggta
    28861 gattgttttc gcttaatgct tctttatagc cttttagacg gttttgacca ttgataggat
    28921 caatcagcgc accgctgaca aaagcgattt ttttattgcc gctttttgct aatttattta
    28981 ctgcatcttt tgttgctgac acataatcaa tgtttacgct acctacttgt tcatctggat
    29041 caatggaacc agctagtaca acaggcgttt ttgaacgtga aaattctcca cggatttcgt
    29101 ctgtgatatg gtgacccata aagatcacgc cgtccacttg cttagccaat aaattattca
    29161 atacattgac ttctttttga tcgttgccat ctgagtttgc taagatgata ttgtatttgt
    29221 acattgttgc cacatcgtcg ataccacgtg ctaaagaggc aaagaacata ttgctgacat
    29281 caggaatgat taccccaact gttgttgttt tcttacttgc caagccacgt gcgactgcat
    29341 ttggacgata atccaagcga tcgataactt ccaagacttt ttttcttgtt gctggtttga
    29401 cgtttggatt gccatttacg acacgtgaaa cagtagccat cgaaacattt gcctctctcg
    29461 ctacatcata gatagtgatt gtttgctttt ccatttgtgt tctcctattc atattttata
    29521 tgttttctga aaatacgtct tacatttcta aaataggata tcaaataatc tcaacaattg
    29581 caaccgtttt atctctgttt tcatgaaaat ttttactttt ttcagtttta agaacgttgg
    29641 tcttttaatc aatcagtggt acaattattg aatacaaaat taatttagaa agtaggttgc
    29701 caaatgaata acgtaaaaat caatgaacta cgtcaatgga tggcacaaca ccaagtagaa
    29761 ttggcttata tcagcgaccc tggccacatc gcttactttt ccggatatga aagtgatcct
    29821 cacgagcgtg tactagctct ttttatccct ctggaacaag atcctttcct tttcactcct
    29881 gctctggaag ttgaagatgc tcaaaacagc agctgggcgt acgatgtacg tggctactta
    29941 gacagcgaaa atccttggga gatcatcgga aaagagcttc gcgcacgcta tgggcaacct
    30001 ggtaatgtag gtattgaaaa gaatgctctg actgtcgatc gcttcgatgc tatctctgat
    30061 atcttgacag gtacagattc ttacaccgat ctaacttcta ttatccagcg tctgcaactg
    30121 atcaaaacgg tagaagaaaa agacaaactg atcgaagctg gtaaatgggc cgatgttgcc
    30181 tttgaaattg gcttcaaaac agtaaaagaa ggcgtaactg aacaagcaat tatcgcagaa
    30241 atcgaatacc aattaaaaaa acaaggtgtt tcacaaatgt ctttcgacac attagtcctt
    30301 gcaggagcaa atgctgcaag ccctcatggt acaccaggaa gcacgaaggt ttcaccaaat
    30361 gaactagtgt tattcgatct tggcgttatt tggaatggct attgcagtga tgctactcgt
    30421 acagttgctt atcaaaaacc aactgagttc caagaaaaaa tctataacat cactcttgaa
    30481 gcacagcttg ccgcacaaga ggcagttcgc ccaggtgtga cagcaggtga acttgatcaa
    30541 atcgcacgaa acgtgatcaa cagctatggc tacggcgaat atttcaatca ccgtttaggt
    30601 cacggaatcg gaacaaccgt ccacgaattc ccttctcttg tagaaggaaa cgacttagtg
    30661 attgaagaag ggatgtgctt ctctcttgaa ccaggaatct atattcctga aaaagtaggg
    30721 gtacgtatcg aagattgtgt gtatgtgaca agtgatggtt gtgtgccatt cacgactaca
    30781 ccaaaagaat tacttgtttt agagtaatga aaataaaaaa ggtgagagct ggtccgctct
    30841 cacctttttt attctagaaa tgagtaatct tagtgattgg tagtcgaatc tgtcattgta
    30901 tctttcattt cttttttagc ttcttttgca tcttcttttg ccattttcaa catttctttt
    30961 gtgtcatctg caactggttt tgcttctgac attccttcat tgaccgtctc tcctgcttta
    31021 tctgcagcat cttttacatc gatcatgata tcttctgaag tatctttagc aatactacct
    31081 aattcgtctg ctggcttttt cagatcttct gctgtttttt tgaaacgatt agataaatca
    31141 gctgattgtt ctttgaggtt taacaagata tcatctgatt cacttcctgc ttcctctgct
    31201 gattcactta ccttctgctt aacattgcct gctagatccc ctgcttgttg agaaagacct
    31261 tgcgcttttt cttttgcaat acctgttaac tcagatgatt tttcttttgc catatctgtg
    31321 taatctgaag ccatgtccat caaatcatct gcttgagcag ccaattcatc ccgaagttct
    31381 ttaccagatt tgggtgctaa caataatgcg gcgattgctg cggctgtacc tccaactact
    31441 gcacctaata aaaatccacc ttttttagcc attcttattt tcctcctgtc gtttcagtat
    31501 gttttttgtt tttaaaaaat ttcatcgctg ttccaccgac ttttccggca acagtcgctt
    31561 tagctgtcgt ttttccgatt ccgccgactt tcgtcacgat gtttttacta gaatgattaa
    31621 gttccgatac actttcactt aaatcagcaa ctgctgtgaa caatggatcg atcgttgcga
    31681 cttttccatt gatatccaag agcagctcat tacttttgac tagcaagcct tctacttgtc
    31741 tagataaaag atctacgtct tgtgtgacga tctcaattgt cgtatttgct tctgcgacag
    31801 tctggtctac tttttctaaa gttttggaaa cttgcaacaa tacgcgaacg atgaaaacga
    31861 caagtatcgc aaaggcgatt gctgcgatca atcctgcaat ttctcctcct gacatgatca
    31921 aactcccttc ttgtctctct tttctataag gatagcattt tcgtgttttt taaacaaata
    31981 aaaacgagct tcaactactt tttattcaat tagcagctga agctcgttag aaaaaaagat
    32041 tattttttct tttcttcttt gatttctgtt attccttttg gattatgatt gatcttgctt
    32101 ccttctgctt ctaatgtttt acctaaagac atgatgtatt cacgcaaagg tgcccctact
    32161 tctggatgtg tcagaccata ttcaatactt gttttcataa aaccaaattt gtcaccaacg
    32221 tcataacgtt tacctgtgaa tttacgggca aatacgcgtt gtgttttatt caacgtatcg
    32281 attgcgtcag ttagttggat ctcatttcca gcacctggtt gttgttctga taatacatca
    32341 aagatttctg gagtcaataa gtaacgccca ataatagcta aatcacttgg tgctttgctt
    32401 ggatctggtt tttcaacaaa gtttttgaca ttgtataacc catcttccag tacttcccct
    32461 gggttgatga ttccgtattt tgaagtttca tcgtgaggaa cttgcataac ggcaatcgta
    32521 gaagcgtgtg tacgctcata gtcatcgatc aattgctgtg tcaatggtac ttcgtcttcc
    32581 attatatcat cgcctagcat aacgacaaat ggttcatttc caataaaggc tcttgcttga
    32641 agaacggcat gtcctaaacc ctttggatga gattgacgga tgaaatgcaa gttgacatct
    32701 gtcgtttctt ctactaattt caataattcg gtctttcctt tttcacttag gtttgtttct
    32761 aattcaatat ttgaatcaaa gtgatcttcg attggtcgtt tagcttttcc tgtaacgatc
    32821 aagatgtctt cgatacctga tttcaacgct tcttcaacga tgaactggat tgttggttta
    32881 tcaacaattg gcaacatttc ctttgccata gcttttgttg ctggtaaaaa tcttgttcct
    32941 aatcctgctg ctggaataac tgctttttta actttcatat acattcgctc cttcaaaaaa
    33001 ttgttaatga gaattcattt tctttttttc catcacgtaa catgatttct tttgcggctg
    33061 ttttgatttc tttttgttca tacagcactt catagattgt ttgagtgatg ggcatttcga
    33121 tttccatcat ttgggctagt tccatcgctg cttgggtagt attcacacct tcaacgacca
    33181 ttcccatatt ggctagcacc tcttctaggg gctgcccttg tcctaacaag ttgcctgcgc
    33241 gccagttcct agaatgaaca cttgtgcaag taacgactag atcgcctatt ccgcttaatc
    33301 cgataaaggt cagcgggttg gcacccattg ctacacctaa acggctgatc tcagctaatc
    33361 cgcgcgtcat gattgctgcc tttgcattat cgccaaaccc taagccgtga atagcgccag
    33421 caccaatagc aataatattt ttcaaagctg cccccatctc tacaccgatc acatcaggat
    33481 tggtatagat ccgaaaataa ttattcatga acagctcttg tacatatttt gctgcttttg
    33541 catctgtact tgcagctgtg atcgtagtga tgtcatgttt tgctacttct tctgcatggc
    33601 ttggtccaga aagaacaaca acagcttttc ttttttctgc tgggatttcc tctaacaaga
    33661 tgtcagagat ccttttatgt gttccctgtt ctaatccttt acttgcatgg atgatcactg
    33721 gctttgtatt caatttaggc actagttctt ttgcaacaga acgtattgct ttcgttggaa
    33781 tgacgaatag caccgcatct gcgtctttta ctgcatcttc catattggtg aatgcttgga
    33841 tggaatcggg tagataatga tctggtaaat aacgttgatt cgtgtgttgg gtgttgattt
    33901 cttctacttg tgaagcacga tgtccccaaa tccgaacgtc atgtccgttt tcggcgagga
    33961 cttgtgccaa agcagtaccc caagagcctg gtcctaaaac cgctactcta taattcattt
    34021 taccactcct gaaatttttt tcatttttaa cgctctaaaa atcagataat ttgttgattt
    34081 tttcctaatg agcggtcata atccggtaaa tttccttttt ttctgcgcca aataaataga
    34141 atcaatgtac cgacaaataa cacagcagat aataattgag aaatacggat cacacctaaa
    34201 aagtaaagac tgtctgtacg taatccttca ataaagaaac gcccgaaact ataccacatt
    34261 agataacata aaaggacttc gccttttttc aaaaggtgtg gtttctttcg taaaaacaaa
    34321 agcaaaataa atccaatcaa actccaaagt gattcataaa gaaatgttgg ttgacgatag
    34381 atcccttcga tatacatatt atcaatgatg aattgcggta aatgcagact ttctaagaag
    34441 cttcgcgtcg tgtctggtcc atatgcttcg tggttcatga aatttcccca acgaccgatt
    34501 gcctgtgcta ataatacact tggtgcagcg atatctaaaa aagtccacaa agaaataaaa
    34561 tgatatttag taaaaaaata cagcgtgatc gcgcctgcaa tcagtccgcc ataaatagct
    34621 aagcctcctg aacggatatt gaatatttgg attggatcag ctatatactg gggaagatca
    34681 aataaaacat aatataagcg agcgccaata atggagatag gcaatcccca caacataaaa
    34741 tctgtcacat catctgcttt taatcctacc cgaacagcct ctctgctgct aagccacata
    34801 gcgataatga tacctgatac gatgatcaat gcataccagt agataggaat ccctacaagc
    34861 tcaaaagcaa ctcgattgat ttgtgctaac atattgtcac tccttgctag tcgttgcctg
    34921 agttttcttc aatcaagcga cttaatcttt cttcaaatgt tttcgttgcg tcaaagccca
    34981 ttgtacgtgc acggaaattc atcgcggcta cttcaatgat gattgcaacg tttcgtcctg
    35041 ttttgacagg gatttttatt tgtggaatgc ccacttccgc gatttcaacg gtcgtgtctt
    35101 cagagcccag acggtcgtat ttcttatctt ttgcccatgc ttccagataa acagccaact
    35161 ggacttgcat agagccacgt accgcactcg caccaaaaag attcattaca tcaatgatcc
    35221 cgattcctcg gatttcaatc agatgttcca ggattttagg cggttctccg atcaccgtta
    35281 attcatcttg ctgataaaca tctactcgat cgtctgcgat caatcgatgg ccgcgtttga
    35341 tcaactccaa cgctgtttca ctttttccta tcccactgtc accttgaatc agtacaccta
    35401 atccgtaaac atccactaaa acaccgtgga cacttgttcg aggggcaagg cgaccatcta
    35461 aataactgga taattctcct aataaacgag aagttgagat gggagaacgc agtacagcta
    35521 tcccattttc atttgctgcc tggatcattt cttctggagc tggtaatcct cttgaaataa
    35581 taaatgcggg catatcttca ctacataatc ttctcatgat gatcaaacgc tcttctggga
    35641 tcatccgttc cgcaaaactg atttctttac tgccaaatag ctgtaaacga ttatgggaat
    35701 aataattaaa ataaccagtc aactctagtc ctgggcgtga gatgtcccca gttacgatga
    35761 cacggtctaa gcttttctcg tcgcctgtca cgatttccaa agataatttt ttcactaatt
    35821 ggctaacttt tacaacttct gacattcatg ctacctcatt tctattagag tgttctctat
    35881 cattttatca ttgtctagcc aaaagtacta ccaattaaaa aaaacgtttt tatttaatta
    35941 ccaatgtaat agaaaacgca ttcctgattc taaataaaaa aactgcgccg atagttcggc
    36001 acagctttct tttattttct actgttatgt tcgctgacaa ttacattgac aagcgacatc
    36061 aagatagcta ctaacaacgc tgcaccgaat gaagaaaaac taaaatgttc tgctcccaca
    36121 aaaaaggagg tcattttaag aatcgctgca ttgataacaa acgtaaacaa tcccaatgtg
    36181 actaatgtaa agggcaggga cagtatcgtc aaaatcggtt tgacaaacat attcaacaca
    36241 gacaatacga agctggcaag aacggccgtc caaatactgc tcacatggac catcgcagga
    36301 aaaagaacag ccaatgatat gaatgttaac gtattaacta aaatacgttg aaagtacgac
    36361 attaaaagtc actccattcg tcatcatcga ttttttccgc ttctttgcgt ttttttgttt
    36421 gttcacgagt ttgttcacga taattgtttt tttcataata tgcattgtta tgtccatagt
    36481 ttgttcgtgc agaacgcgca gatgggataa tcaaagctaa taaaatgtat aaaataattg
    36541 gacttccaaa agcaactaag ctcaggaata cataaagcac acggataaca gtcggatcaa
    36601 tcccgatgta ttctcctatt cctgctaaag tccctgtcaa aacaacattt ttattagatt
    36661 ttgtcaactt ttttttcatc atgtctcacc tctgttctac tattttaaat gaagaaaatc
    36721 gttgtgacaa ccttcttctt aatcggtgtc tttctgcaaa attctcggtc acaaattacc
    36781 atattcataa aacgtgacga gctttttctg aaattattcc ttgtgactcg gcgataattg
    36841 gcaggttcat aatctcttgg taaacggtgt tcaagagata gactatcggc aataaggcac
    36901 aattttgtaa aataggggaa gctattttac aaaatcgctt cttattgctc aaggctgaac
    36961 atctctttca caacctcttt atttatctgt atcttttaaa tagatacttc ctgttgtcgt
    37021 tgaaagttgc acttgcgcga tctcatcatc tgaaacacga cggaattgca acatttggtt
    37081 tgtccgttct tttttctcac gaactacttc gtaattggac agacggctat tgatacttcc
    37141 aagacttgtt ttagcatgtc cttccaaacc gatgccatct ggtaaagcga ctttgacatt
    37201 tccgttcact gaacttgctt ctactttttt caaattatct tctttaatcg taagacgtac
    37261 atcaccattt actaaagaca cagaaagatt ttctgctgtc gcaccaaatg tcactgtacc
    37321 atttactgtt tcgataatag aatctaagat gttcccgttt cgaatatcga tattgccgtt
    37381 tacaccttct acttccagca tcgttgcagt taattggtcc acgatgatgt taccattcgt
    37441 agattttgta taaatatctt tagcttccaa tgtttcaatc atgatgttgc catttagaag
    37501 tttaatagct gcgtggtcgt atactcgttt aggtaagtag aaaacaaggt ctgcacgcac
    37561 acgtttattt ggaatttgga aggagatatg atcttcatta acctcaatct ggctccgttc
    37621 agagaatgct tcaaatggtt cagcacccat ttttccgtaa agtttgatct tagcttcaac
    37681 tttcacatcg ttgctgtccc acgtttttaa cgtcacgttt ccgtttgctg ccttgatatc
    37741 aaggatactt gctgcaggag cttcatagta aaattcatgc tcaaatttcg ttgtcgcaat
    37801 cccagggaca cgtaagctga catctttcca ttccatgttg tcattcactg tttctgaaac
    37861 agtttggaat gttttcttca agaatttgcc taactgtgat ccagcttctg acattttttc
    37921 gcctacttga ttcagtgtat ctgttgcttg atctttccaa tcatctggca attcaaactt
    37981 atcagtaaac gtttcttttt tctcagacca ttgattctta cggatatctt ttaattctgc
    38041 ttctaatttc aattgttctt ctaacagtgt atcgccacta gcttctaact ctttgatttc
    38101 tgcctctaac gcctgacgct gtgctaattc ttcttctgtc aactcgccta attcttcttt
    38161 tgtattcagc tgcatcaatg cttcctgttt ctcattgcgt gctgccttat tgtcagcgaa
    38221 ttgagcattt acttcatcta gacgcgccga agttttattc gcttcagttg ctagttcatc
    38281 taagatcttt tctaagtttt cttgatcttg tctttctctt tcctttggat caactggctc
    38341 agaaatttct tcttcgccgt tttcaagttt atcgatcaat tgacttgcct tatctttttc
    38401 tttatgacta gcagttacct tgtctgcttc ttttttgatt tgcttttcgt ctttttcagt
    38461 tgccatgctt tctaataaat ctaagccttc ttcagtcgaa aggattcctt ttttcactaa
    38521 atctaaaata cgttctcttt ctttcatgga aattgcctcc ttaagcaaaa ttgtttgcaa
    38581 gcttttcctt acatccatat tatgcactga ttcagtaaaa atgtcatagg acttaagttg
    38641 taacttttac tccatcttga gttgttcttt cttttttgta aagagatatg ctgtagtcac
    38701 gcaggttaaa ggaagaacaa ttagtgcgga caatgtggta agaatagcca tcgtatattc
    38761 tagaacaaat gatttttcat tgatcacttg ctgaaaagga atgtctaaat caataaacca
    38821 gaggatcaac gctaaagaac ttcccatgaa tgcaaaaaga agtgtcatta ctgttgacgc
    38881 caatattttt ttagcaatag cgaaactaga atggcgcaat tggtaaaaag ataattgcgg
    38941 atttgcctga tatacttcga atgtcccact agaaattgct acactcgcat ctaaaacagc
    39001 accactaaga ctaatcagaa gcaacgatct cgtaagtaat ctaaaatcaa tcgctaaagt
    39061 cttatctaag ccagataatt cttctagttc taccgatgta aaaccttgga tcgctaaaaa
    39121 atcagtgata ggaagcaata gtaggaaaca aaataaaaag atcagtatac tcacaaaagc
    39181 tgcacgcatt tttagattat aaccgttgat aaaaaataag ataaccacag caatcaataa
    39241 aaatcctatg accgacaatg ccagtatcgg ccacttctga tgataaccga ataacaacag
    39301 gaaaaagatc cctaaattga tgaataagcc taaaagaata tacatacctt gcgtaccgcc
    39361 aataagtagc acgctgattg ctaaaatccc aacaagtata ataagtacat tcatcgtttc
    39421 agcctcctta cagtcagaaa aaagaggacc gagatgggga tcgataatac tattcctaag
    39481 ctgccagcaa tgacgcgtaa aaattccaat gataaatgct gttctatcgt ttctttgaaa
    39541 gaccattgat ttcctaaata taaaagaagc gatggaactg acccactgat atagacgaac
    39601 aaaaggatat tggtcatcga gccaatcaca tctcgaccca cttgccttcc tgaaagccat
    39661 aactgtttcg ctgttatttc tggttgtcgc ttagtcaatt cttctaatgt cgcaatgact
    39721 gtgataacgg tatcgagtgt tcctccagct gttccaatca gtacgctaga aagaaaaaca
    39781 gaacgatacg gtcgtgtcag ataattcagt tcctcatagc gcagtccttg gtctttccaa
    39841 tgattcattg tcaaaagaca aataaaaaat gctaaaaaaa cactcgtaac tgcagctgaa
    39901 attttgatta tactgcttct attccatcca tctaaagcca atatgctaat aatcatccct
    39961 ataacgacga acaggataga tttatttaat agagaaatcc cttctgtttt agtaaagatg
    40021 agcaacaaaa gtaaaagtaa aacgatattg atgatgagac ttcctaaagc aaatagaccc
    40081 tgttttccgc caataagcaa catggcgcca atgaagatac ttgtgaagaa gaaaagccag
    40141 ccatcatttt tttttgctac tagctgccat tcatctccat gcatttggac tagatacatt
    40201 tgtctcgctt ttatatttgc tgtttctatt tgattttttt gataaggaac cgataaataa
    40261 atcgattctc ctttttgatc cgagttcatc agttttcctg taatcaattg atgatcctct
    40321 tttattcgta ctttttctat ctcgatgaca ggtacttcat ataaagcggc ttgatgctga
    40381 ataacccatg aagtaagcac acaaataaaa aatatagtga tgatgctaat t
//
LOCUS       DAJSKS010000008        36925 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJSKS010000008 DAJSKS010000000
VERSION     DAJSKS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.008"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            45..1412
                     /gene="nhaC"
                     /locus_tag="ON396_000405"
     CDS             45..1412
                     /gene="nhaC"
                     /locus_tag="ON396_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCR3112539.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1693..2712)
                     /locus_tag="ON396_000406"
     CDS             complement(1693..2712)
                     /locus_tag="ON396_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3112540.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(2734..3741)
                     /locus_tag="ON396_000407"
     CDS             complement(2734..3741)
                     /locus_tag="ON396_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR3112541.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(3734..4399)
                     /gene="pgmB"
                     /locus_tag="ON396_000408"
     CDS             complement(3734..4399)
                     /gene="pgmB"
                     /locus_tag="ON396_000408"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR3112542.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(4392..6686)
                     /locus_tag="ON396_000409"
     CDS             complement(4392..6686)
                     /locus_tag="ON396_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCR3112543.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            6946..9111
                     /locus_tag="ON396_000410"
     CDS             6946..9111
                     /locus_tag="ON396_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3112544.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            9415..10248
                     /locus_tag="ON396_000411"
     CDS             9415..10248
                     /locus_tag="ON396_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR3112545.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(10350..12050)
                     /locus_tag="ON396_000412"
     CDS             complement(10350..12050)
                     /locus_tag="ON396_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112546.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            12557..13717
                     /locus_tag="ON396_000413"
     CDS             12557..13717
                     /locus_tag="ON396_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3112547.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            13733..14176
                     /locus_tag="ON396_000414"
     CDS             13733..14176
                     /locus_tag="ON396_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3112548.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(14659..15189)
                     /locus_tag="ON396_000415"
     CDS             complement(14659..15189)
                     /locus_tag="ON396_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HCR3112549.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            15294..15845
                     /locus_tag="ON396_000416"
     CDS             15294..15845
                     /locus_tag="ON396_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3112550.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(15998..16471)
                     /locus_tag="ON396_000417"
     CDS             complement(15998..16471)
                     /locus_tag="ON396_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3112551.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            16609..18081
                     /locus_tag="ON396_000418"
     CDS             16609..18081
                     /locus_tag="ON396_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR3112552.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            18078..18788
                     /locus_tag="ON396_000419"
     CDS             18078..18788
                     /locus_tag="ON396_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HCR3112553.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            19140..19424
                     /locus_tag="ON396_000420"
     CDS             19140..19424
                     /locus_tag="ON396_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HCR3112554.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(19741..20124)
                     /locus_tag="ON396_000421"
     CDS             complement(19741..20124)
                     /locus_tag="ON396_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HCR3112555.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(20126..20710)
                     /locus_tag="ON396_000422"
     CDS             complement(20126..20710)
                     /locus_tag="ON396_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR3112556.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(20757..22232)
                     /locus_tag="ON396_000423"
     CDS             complement(20757..22232)
                     /locus_tag="ON396_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HCR3112557.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            22413..22724
                     /locus_tag="ON396_000424"
     CDS             22413..22724
                     /locus_tag="ON396_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112558.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(22839..25175)
                     /locus_tag="ON396_000425"
     CDS             complement(22839..25175)
                     /locus_tag="ON396_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3112559.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            25317..25985
                     /locus_tag="ON396_000426"
     CDS             25317..25985
                     /locus_tag="ON396_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3112560.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            25988..27031
                     /locus_tag="ON396_000427"
     CDS             25988..27031
                     /locus_tag="ON396_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3112561.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(27268..28149)
                     /locus_tag="ON396_000428"
     CDS             complement(27268..28149)
                     /locus_tag="ON396_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3112562.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            28308..29036
                     /locus_tag="ON396_000429"
     CDS             28308..29036
                     /locus_tag="ON396_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR3112563.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            29192..29509
                     /locus_tag="ON396_000430"
     CDS             29192..29509
                     /locus_tag="ON396_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3112564.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            29502..30110
                     /locus_tag="ON396_000431"
     CDS             29502..30110
                     /locus_tag="ON396_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCR3112565.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            30165..30956
                     /locus_tag="ON396_000432"
     CDS             30165..30956
                     /locus_tag="ON396_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCR3112566.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            31094..31582
                     /locus_tag="ON396_000433"
     CDS             31094..31582
                     /locus_tag="ON396_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HCR3112567.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            31633..32505
                     /locus_tag="ON396_000434"
     CDS             31633..32505
                     /locus_tag="ON396_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCR3112568.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            32681..33043
                     /locus_tag="ON396_000435"
     CDS             32681..33043
                     /locus_tag="ON396_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR3112569.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            33052..33804
                     /locus_tag="ON396_000436"
     CDS             33052..33804
                     /locus_tag="ON396_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3112570.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            33818..35620
                     /locus_tag="ON396_000437"
     CDS             33818..35620
                     /locus_tag="ON396_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3112571.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            35897..>36925
                     /locus_tag="ON396_000438"
     CDS             35897..>36925
                     /locus_tag="ON396_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR3112572.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKIN"
BASE COUNT        11440 a         6547 c         7630 g        11308 t
ORIGIN      
        1 ttttttcatg gcagggcctt ttcatttgaa ataggagatc gacgatgaat aaaaatattt
       61 ctttaaaaga aagtatcgct gtattgatcg tgttgttggc actcctagga gtactgatca
      121 tcggattcca gctctcgcca catatcccga tccttgcagc tttcatgtgt ttgttgtttt
      181 atggcagatt taaaaagttc acatgggatg agatccatga cgggatcatc aaaggaatca
      241 ctccagggat cattccaatc ctgatttttc ttttgattgg tgtcttagtt gcttcatgga
      301 ttttatctgg aaccatccct acgattatgg tttatggatt taaacttgtt tctgtgcgtt
      361 tctttttacc gacagcattt cttgtttgtg caattgtcgg agtgactgtc ggcagttcat
      421 ttacaacgat ttcgacgatt ggaattgctt ttttggggat cggtcattta cttggattca
      481 atgatgcaat gactacaggc gcagtcgttt ccggtgcctt tttagggaat aactgctcgc
      541 ctttatcaga tacgaccaac ttggcagcgg gtattggagg agttaatcta ttcgaacata
      601 tcgctggtat gcgttatacg gatttcccag cattcgtcct ttcgttgatt ttttatacga
      661 tattaggaca taacagtcat tcagccgatc tagctagtat caatgaaatg atccaaaaca
      721 tgaaagcgag cttttggatc tctccttgga cattgattcc actagttatt ttatttgggg
      781 gggcaatcaa aaaaattccg gctatcccta cattactagc gggatcgaca gctgctctcg
      841 ttctttcttt tatccatgag aaaagtttga cggtcggaaa agcagcagac atcttgatga
      901 atggttatgt cgcaaataca cctgatccaa agctagatga actgctttct cgaggcggga
      961 tcatgagtat gttaggttct gctagtttga ttttacttgc attggcatta ggtgggttac
     1021 tgatcaaata tctgatcgtt gataccatcg ttcaagaatt aaaagaaaag atggaccgac
     1081 cttctcgttt agtcgggttt actgccttga gctgtatcgg aatcaatctg atcgttggag
     1141 aacagtatct gtccatcatt cttccaggag aaacattcaa gcgttctttt gatgacgttg
     1201 gcttatcaaa aacctatttg acacgaacac tagcggattc aggtgcgacc gtcaattctc
     1261 tcgtaccgtg gggagtcagc ggcacattca ttatgggaac gatgaaagtt agcgcgttgc
     1321 attacttgcc attcgccttt ttctcgattc ttgcacctgt ctttacaata ttaggcggtt
     1381 ttttattgaa tcgtcgacgt gtaaaaaatt gaaggaagga aaaaagaggc tgggacagaa
     1441 gcgtttaact ccaagaaata agaagaaact caagaaaatt gctttttaaa tttttgtgaa
     1501 tttcagctta tttccgaagg agttgcttct gcttccgccg tttatacgtg tttagagcgt
     1561 gagacaaaag tgttctttac ttttgtccca cgctcttttt tacagataaa caatgtgtta
     1621 gaagtagtat cttcttttac ataaaaagct gaccatagga taaatggtca gcgtatttag
     1681 tgagtcagct atttagtatg ggttttctaa aggaacgact gtctctcttt tgatcaagcg
     1741 atgagggatg accaaacgta cgccactgct caattcttca tggatcaatt cgcgcagctt
     1801 ttgcatagcg acacgtccta attcggagat atcgatatcg atacttgtaa ggtaagggtg
     1861 tgttaatgtt gagaagatcg agttgttgaa gctaatgacg gagagtgtct ctggaatgac
     1921 atagccatac atttgcgcca actgcatcgt gcgaagggcg aagatatcgt caatgaccac
     1981 caatgctgtg gcttcgtttt ctttcaacgt tttctcaaag gacagataat cctccggatg
     2041 ctctagtact acactggata acgcaggaag accagccatc atcattgctt tttgataacc
     2101 gaaatatcgt tcgaaataca aactttcatg agtcgtgttt gtcacaaaca ggatattctt
     2161 atgtccattc tggatcaaaa attctgttgc ttgtttacct agaagctggt tatcattatc
     2221 tacataaacg atttcgttct catgatgata aggctgtccg atcaaagtga aagggatgtg
     2281 gttttggtat aaataatcaa tgacagtatc ttgactatct gaatacacta gaataaaacc
     2341 atctacttgt ttttgtaaat gcatacgctg gacgttctct aacagcaatt caaaattttt
     2401 agcagtagcg attgctgtag tcatatcata attccgcgct tcttcgttga ttgcttccat
     2461 gatctcaaga taaaatgggt taccgatccg ctcttttgaa tcaagaggag gtaagattac
     2521 gccgatggca ctggaaatgc gttttcctag attgcgggca gtcatgttag gaacataacc
     2581 tagttcatcc ataatttttc ggactttctt ctttgttgtc tcggaaatac taggatgatc
     2641 gttgatgaca cgagagacgg tcgaggtggc aacaccggct tttttcgcca catcttttac
     2701 ggtaatagcc atgataaaaa cacctttctc ttgttattgt gcagagaacc gataaatcgt
     2761 ctgactgatc cgttcttgac caggatggag gttgatcgat ccaaatttgg ggaaatgaac
     2821 gatatcaggg atttcttgcg gttcgattgc taatccgtag tttgaatgca tctgttttcc
     2881 attgacagag aaatcagctt cgaatccagt cgtagaaaat aataccatac ttttattaga
     2941 agttgctata gtcatttggc gaccagaatg cttctcgaat aattgtaagg aagttttgga
     3001 aagcctagga tgatgcaaaa gaaatacatc atctagtcca tctggataac atgcaaggat
     3061 ctcttgcagt gttggggatt ttctaaagtc gattggcagt ttttcttttt ttgtccaatt
     3121 tccagtaggc agtttttctt gatctaattc aagcacacca tctaaaaaga ccgttaactg
     3181 atgttcatag atgtctcttg ttccatttcc tgaaaggttg aagtaggcat ggttggtcgg
     3241 attgcagatg gtttcaacaa ggctactagc agaggttgtc atctctaatt cgttttttgt
     3301 taaacggtaa gtgatcgtag cagtgattgg tcctggatag gtagaaaatt catcttttaa
     3361 gtaaaagacc acacctattg atgatggagt tttgaaaact tctacttccc aaaattggaa
     3421 agaccagcct ttcgtgccac cgtggatatg gtgtgatccg cagtttttct ccaatctatg
     3481 ctcaccccaa gaagcatccc ggatccttcc ggctacaggt ccgacagttg ctccaaaaaa
     3541 gcttttgtct gttagaacat cttcgaaacg atcgtaagac aataaaacat tttctgattt
     3601 tccttctttg tctggtgcga agatttggtg caggcgggca ccgtaattga gaagaacgac
     3661 ttctaagtag tcgttcttca atgtgatttt ctttatttca gtctctggaa ctggtacgac
     3721 ggagatctct ggattatcca tggtcatgcc acttttcttt caagtaatcg atcgtaagaa
     3781 aagtcgtatc agggacaagc gggatctcct ctccaaggtc ttcagcacgt cccacgccaa
     3841 ttggttgagc gccacttgag aggatggctt ggataccggc ttgcgcgtct tcgatcccga
     3901 tacattcttc tgcttttagt ccgacagctt ttgctgcaag gaggaagata tctggcgcag
     3961 gttttccgtt tgttgcttct gccgggtcgg caatagcatc aaagtaaggt gtaagaccca
     4021 ttttttcaag taggaatggc ccgtttttac ttgcagaagc aagagcaatc ttgatttttt
     4081 cttcttttaa agaatccagt aaaggcacga ccccggggaa gacatctttt ggttcgatcg
     4141 tctggatcat ctctaaataa taatcgtttt ttcttttcgc taattctgca aattcttcct
     4201 ttgaaaaaga gtgttctttt ccgccataag ctaaaagcaa agagagtgag tcttctcggc
     4261 tgacgccttt tagctgttcg ttgaatgtgt ggtcgatcgt aatgccgatt tcctgaccaa
     4321 gctttcgcca agcacgataa tggaattcag cagtatcggt aatgacaccg tctaaatcaa
     4381 acaatacccc tttaaacatt tgcttcctcc tttcttagcg ttatttctag acgatccgct
     4441 aaagaataag actgttcata gacgtaaatt tctagaggag aaccttctaa taactcgaaa
     4501 gtaacttgtt catctgtgac tgagatgaac aataaacgtc cgcgataatt gatatggaaa
     4561 gaataagctt tccaagtttt tggcagaaat ggtgcgaatc tcaactgatc atgggcagtt
     4621 ttcatttgag caaatccttg gacaatcgac aaccagctac cagtcatgga agtgatgtgc
     4681 aagccgtctt ctgtgtcgtt attgtaatta tccaaatcca agcgagctgt tcgctggtac
     4741 atttctacag ctttgctttc cattcctaat tctgctgcta aaatcgaatg gatacttggc
     4801 gaaagggaag actcatggac agtcattggt tcatagaaat caaagttttt ttgcttttct
     4861 tcttttgtaa aagtatcatt gaagaaataa atcccctgta gtacatctgc ctgcttgatg
     4921 aagcaagatc taaggatttt gtcccaagac caatgctgat tcagaggaag atcgcttgtg
     4981 ggaatatcgg atacaggcat gagatcttta tctaagaatg tgtcatgctg gacaaagatt
     5041 cctaattctt cgtcaactgg gaagtacatg ttctctgcga tatctttcca tttggctagt
     5101 tcttcttcgc tgatagtgat agcagcttct ttttgataag ttaaataatt ttctcgcgtg
     5161 taacgcaaga cccaagctgc gatcgtattt gtataccaat tgttattgat attgttctca
     5221 tattcgtttg ggccggtcac accgtggatc atgtatttat ttgctcgttt tgaaaaatgg
     5281 acacggtctg cccagaagcg tgcgatttct gctaaaactt ccagtccttc atttttcaaa
     5341 tattccgtat cgccggtgta attcgtataa ttgtagattg cataggcaat cgcgccattt
     5401 cggtgaatct cctcgaatgt gatttcccac tcgttatggc attcgacacc agtgaaggtg
     5461 accataggat aaagcgcgcc ttttaatcct tgttgctgag cgttgtggat tgcttgcggc
     5521 agttgattat gccgatattt cagcagattc ttagttactt caggtttggc taaagcaaga
     5581 taaagaggga cagcatacgc ttctgtatcc cagtaagtgg ctccgccgta tttttctcct
     5641 gtgaatcctt tggggccgat attcaatcgt tcatcttcgc cgtagtaagt agagaatagt
     5701 tggaacaaat tgaatcgaat accttgttgt gcttcttcgt ctccttcgat gaccacatcg
     5761 gcaagcttcc atcgttctgc ccaagccgct gtttgctctt cttttgcttg gatataattg
     5821 tttgtctggt tcgttaattc tgagacagct ttttctgctt gctgcgcttc tggaatatct
     5881 ctactagtaa gaacgataat ttctttttcc actgaccatg tctcaccttg agataattct
     5941 gcagacaagc aaccatgtaa tgctaaagta tcttcgtgat agtcagggat gactgatttt
     6001 ccattaacga aatggcgcat tgctgcagta acagtaaatt gttcaatccc aaagttgttt
     6061 ggaatagttt tagtcgtcaa atagccgatt tcttctatag ttccacgatc aatctcattc
     6121 caaaaatgtt catcatagtt gctgtcttcg ttttgaacat ttccatccaa ttttgatacg
     6181 agtttgactg ttccgctacc ttcaagcatt tctatagtta aatagatata agctgattct
     6241 tttttgacaa tgctcagaaa acgttcgaag gaaatgcgta ctttattagt agaagtagtg
     6301 attgtgaatg tacgagataa tacaccattt ttcatatcta attcccagta aaagtcttct
     6361 ggttccaaag aagccaagtc gatcagctga tcgttgatcg aaatatctat cgcgataaag
     6421 ttcaaggcgt tgatcacttt tccaaagtat tctggataac catttttcca ccagccgaca
     6481 cgtgtcttat cgggatacca gacacccgcc aaataagtac cttgatggtg gtcccctgag
     6541 taagtttctt caaagttccc gcgcatcccc atataaccgt ttccaatact tgttaaagat
     6601 tcttgcagtc gaagattttt tttatctaat tgtgtcgtgc tgattttcca tggatcaata
     6661 gcaaatagtc gtttgatctg tttcattttt ctcctcctaa tgagctgttt tatacgttca
     6721 taataaacgc aaccgattgc acaagtcaat agacaaaaga aaatatttca tagaaaaaag
     6781 cttaaaaaaa taaattttta ggagaaaatt atagaaggga acacacgtat tataggtttt
     6841 atagaatggt atagacagaa aaataaaaaa taaagcattt acaaaaaaga aaacgcacgc
     6901 tataatacgt ttaacgaaac cgattgcgta aaggagctaa acggaatgaa aaagatattt
     6961 agttttgagt tttggcaaaa atttggaaag gctttaatgg ttgtggtagc agttatgccg
     7021 gcagctgggt tgatgatcag tatagggaaa tcaattccct tgatcaatcc tgattggacg
     7081 ccgcttgtca caacgggcgg agtaattgaa aatattggtt gggcaattat tggtaatctt
     7141 catattttat ttgctttagc aataggagga agttgggcaa aagaaagagc aggcggagca
     7201 tttgcagctg ggatctcatt tattttaatc aatcggatca ctggggctat ctttggtgtc
     7261 acaagtgaca tgcttgcgaa tgaagatgca tttacccaca ctctgttcgg tacgaagatc
     7321 atggttaaag gattttttac aagtgtattg gaagcaccag ctttgaatat gggagtattc
     7381 gtagggatca tcgctggatt tgtcggggca atggcttata ataagtacta taattataga
     7441 aaattgccgg atgctttgtc atttttcaat ggaaagcgct ttgttccgtt tgttgttatc
     7501 ttatggtcaa caatcgtggc attgattctt gctgtggtgt ggccaaacgt acaggctgga
     7561 atcaataatt ttggtttatg gattgcagaa tcacaagaaa gcgcaccgat tctagcaccg
     7621 tttttatttg gaacattaga acgtttacta ttgccatttg gattgcatca tatgctgacg
     7681 attcctatca actatacaca gcttggcgga tcgtatgaga ttttatcagg cgcacaggcg
     7741 gggacacaag tatttgggca agatccgtta tggttggctt gggccactga tttagtgaat
     7801 cttaaaggtg caggggatat gtcgcaatat gagtttgtgt tgcataactg gacgccggct
     7861 cgtttcaaag taggacaaat gattggttct tcaggtatct tgatgggaat ggcttttgcg
     7921 atgtatcgta atgtcgatcc tgataagaaa gcaagatata aatcaatgta tttttcagca
     7981 gcgttggctg tctttctaac aggtgtaaca gaaccattgg agttcatgtt tatgtttgct
     8041 gctgtgccat tatatgtcat ttattcagtg atccaagggg ctgcatttgc gatggcagat
     8101 atcttgccat tgcgggtcca ttcatttggg aatatcgaat tgctgactcg gacaccgtta
     8161 gctatcaaag cgggtctagg tggagatctt atcaacttcg tgttgtgtgt gatcgctttt
     8221 ggtgttgtta cgtatttctt agcaaatttc ttgatcaaaa aattcaactt tgctacaccg
     8281 ggacgtaacg gaaattacga caatgacagt gaagaaaccg ttgtatctaa cagcggaaca
     8341 ggaacagcag atcagcaagt ggtccagatc attcatttgc tgggaggaaa agaaaatatc
     8401 aaagatgtcg atgcttgtat gactcggtta cgcgtaagtg taaatgatcg agaaaaagta
     8461 gggactgaag aagcctggaa acgagcaggg gcgatgggat tgatcgtcaa agacaatgga
     8521 gtccaagcag tttatggacc aaaagcagat gtcttgaaat cagatatcga agatctgctg
     8581 caatcaggag cagagattcc agagccagaa atggtttcta caggaaatgg acaagcaggt
     8641 agtaagcaat acttaggtat agaacaagaa ctagtttccg cagcttctgg agaagtgatt
     8701 cctttatctg aagtaaaaga tccggtattc tcacaaaaaa tgatgggtga cggctttgct
     8761 gttattccaa catcaagaga agttgttgcg ccgatcgctg gaaaagtgac aagtattttc
     8821 cctagcaaac atgcaattgg gctggaaacg aaagatggta tcgaagtttt gatccatatg
     8881 ggaatcgata cagtccaaat gaatcagcca gcttttgaga ttgctgtcaa agaaggccaa
     8941 gaagtaggaa caggaacgaa attagctgag atggatcttg atgtgatcca aaacgaagaa
     9001 aaagatccaa cgatcatgat cgtatttaca aacgataaag tagaagaagt agtgatcaag
     9061 caattaggta cagtgacagc tggtaaagtg atcggctcca ttaaaatgta aggagaggtt
     9121 gtgaaaaagc tgtcctgcat caagtcataa gaacagccaa acaaaaatag ctcctcctat
     9181 ttttcgtttg gttgggctta ttaccgagaa gcagcagttt gaatactgtt tagtggagag
     9241 gtcgtgaaaa agctgccctg caccaagtga taaaaacagc caaacaaaaa atagctcctg
     9301 gtccattgag cttattactg aggagttagc aggagaacac tatttgctgg agaggttgtg
     9361 acaaaaattt tgtcacaacc cttttgtttc gaaatgaaga caaggagaaa accaatgaaa
     9421 ttcatgacac tcaacacgca tagctggctt gagccagaac cagagaaaaa attgcaagaa
     9481 ttagcagata agatcctgtt agaagattat gaagtgattg ctttacaaga aatcaaccaa
     9541 ttactggaat ctgaagaagt agaacctgcg aagctaatga agttctgtcc ggtaaagaat
     9601 caagtaccta tccgtaaaga taattttgcc taccgtttag ttcagctttt aaaggaacac
     9661 ggaaaagagt atttttggag ttgggaaatg agccacatcg gttatgataa atatgaagaa
     9721 ggaaatgcac tgcttacgaa aaaaacattg gaaagtcagg tccttaccgt ctcccaattt
     9781 caaaagaaag aagattatca gacacgcaag atcttgattg ggaaaacaaa gatagatgat
     9841 caggacattt ttgtctcgag ctgccacttt tcttggtgga cagataaaaa aagtggcttt
     9901 tattttgagt ggaaaaactt ggaaaactac tttttggaaa ctcgcgtacc actttttttc
     9961 ctaggtgatt tcaataatcc agtagactct caaggttatt atactgtaag agaaagttgc
    10021 cttcttcttc aagattcgta tgttgtcgcg aatgaaaaag gaaaagcagc tactgtggaa
    10081 aagaaaatag atggttggga acaaaataca gaaaaattgc gtatagattt tattttcgtc
    10141 cctgaaggaa tgcaggttaa aaaatatcaa cggatttttg atgggatcga tagtccaatc
    10201 atcagtgatc actacggggt ggaaatcgaa ttagatgtga acgaataaaa aatataacat
    10261 ataacataaa aaaactgatt aaaataaaac tctccaaacg attataccgt ttggagagtt
    10321 ttttgatcgg atgtatttat cccttttgcc taagcacgta aaagcttgta tctatgataa
    10381 ataaataaac atagcaaggt aaaccagcca atcaaagtaa gagaaagaat gaaatagaaa
    10441 tagattggcg tttgataatg aagcgtgagc tcgttgattc ctttttgctg cttcacgatt
    10501 ggggtcccga tatctgttac ttcataatca gtcaatgttt ttccattttg ctgtagcaca
    10561 gtccgatcgt agacgataac agggatatga acagttttgt ccccagtgtt tttccacttg
    10621 attgtcaaaa gaccgcctgg atgtgttttt ataaaagttg tttcgttttc tagtatttct
    10681 tcgttatacc ggtcgtattt attgtctttt gtctccttat agattggcag atagtcaggt
    10741 gtggtttttt ggaaaacgaa caagctggta gacatatctt tgctgaagaa tgttttcctt
    10801 gcttcttccg gcgtatcgaa tagataagta tgtcgactga caaatgtttc attttcccac
    10861 ttttccagat gctggtataa gttctggatc gtctgtgctg aaaaaataag aatgccaaat
    10921 atacttaaaa gacggaacca cttttgttca gaccactggt tcagtgaaag ggcaaggtaa
    10981 agtagaagca atagcgtaaa tggcacgaaa aatcgaaatg gaaactggat caaactaata
    11041 aaaggcacat ttttttcgat caatgtcgtc catggaatca agctagtaga aagaaaaaag
    11101 aaaacaagac ttgtgtacaa cacgattttt tggaataagg tgttcttttg ttgaaagaaa
    11161 ataaaaagca aaaaacctga taatatcaat ataggaaagg ccttagggta atggagccaa
    11221 taactgccat ccagatctat cgccttttga ctcatttctc tgttaacaaa gggaggaaga
    11281 atcttattcc cactgtaaac aatagctaat cctgcccaaa tatttgctgt cagaacaaaa
    11341 taaagcaaga ccgacttaat cagttgaagc agtgcttttc ctttttcagt ctggttaaaa
    11401 aagtaataaa gataaaaagg gatatacatc aaagctaaca tgatcgaaga cagtaaatgg
    11461 atctgtgtca taagtcccac tgagacagcg actttgataa cagggaactt tttatgaagg
    11521 acgaaatcag caatagggat catacataaa ggataaaaac aagtccccca gctagtgaat
    11581 ccttggttga tagaccagta ttgaatagaa tacgtactca tgaataaaac ggctactggt
    11641 acacttaccc aatttcgaac attgacatat ctcagtaaaa tcagcataga aaatcctgaa
    11701 ataagataaa gaatgaaatt tgaaagaatc tgataatgaa accagctacc tgcaagcaac
    11761 acgatcaatc cttgtaaata agcgaaaaat gggccgtaaa cggcattcac gatcctgcca
    11821 ctttgctgga atccataaag ggaaggaaaa taatggaaat ccccatattt aatttgttgc
    11881 gcggcatcat agaaccgatt gtaatggaat ggggcatcag cacccaaaat tagtttatgt
    11941 gtgaataaat gaggcatgag aaatgccaaa gcaatgaaaa aaattgctaa aaatgggcca
    12001 tattttttca attgtcgaac tcctttgcaa actttagata aattcatcat atatcctatg
    12061 cgagaggtag gcaagaaatt agaaaattca tgaaaatatt tatgaatttt acaagaaaaa
    12121 cataagtgaa agttctaagg agagaggatg cgacataagt tttgttgcat ctccttgttt
    12181 tgaaacagaa tttttcttaa tactcgaaac aaagtagctt tttcatgacc tctcggtaaa
    12241 cggtgttcaa tcagctgctt ctcggtaata agctcactag cctagaaaat atgagaagct
    12301 attttctgac tagcgctctt attactcaaa gcataacgca gttttcacaa cctcttcctt
    12361 aaacggtgtt caaaagcttg catctgcggt aataagccca acccaacaaa aaatatgagt
    12421 agctattttt ttgggctgta cttattactt gatgcaggac agctttttca caacctcatc
    12481 ggctttcatt ttctattttt ttcctgaacc catataataa aagaaagaac aaaaaaactt
    12541 gcaagggagg acacatgtga agcagagtaa gcaaaaacag tctcgtcgac ttttgtttcg
    12601 tttgttttta ttaatatgta tcgtcgtagg gttactgatc gctttatatc cattttatgt
    12661 ggattcttta aatagtttta ttgatcaaaa acgtatcgaa caagtacaaa aacggaccgc
    12721 agctgaaatt gaagtgcagc gaaagaaaat ggaggagaaa aaccaacgat taactgacca
    12781 aggattcaat cctggtactg atcctttcaa tgaaaagaac agaaatgaaa gtacaacatc
    12841 cagccaatta gaggaatggc tgatcggaag tatcaatata cctaagatcc aattaaatat
    12901 ttcactttat gatcggctca atggtaggat ccttgaaaat ggagcaggcg tcttgcaagg
    12961 aacctctttc ccgcttggtg gaaattcgac gcattcagtg atctctgctc acagcggttt
    13021 acctaatcga cggttattta ccgaattaga tcgtttagaa aacggagaca cgtttatttt
    13081 gactgttttg ggggagaaat tggcttatca agttaaaaat atacaagtgg tactgccaga
    13141 tgacacgtct gttctaacga tttcagaagg agaagactta gttaccttgc ttacatgtac
    13201 gccgtatatg atcaacacgc atcgtttgct agtgacagga catcggattc cttataacga
    13261 atcggtaaaa aaagaagaag gaaaaggaaa tcaggaaaga accatgcggc aatggtttat
    13321 tcttgcagga acgataatag ctgtagtcat actgttagtg tttatcggac gactaatcta
    13381 tcagtataga ttaagtaaaa aagtgcttga tttttcattt attattagtg attcgtcagg
    13441 gaaaccggta aatggtagtt cattcatttt gaaacataag aaaaaaacac ttactagaaa
    13501 tggtgtgccg ttttcagtac aaagcgacca tcatgggaaa gtcaagctgg atcaattgcc
    13561 tggtggaact tatcgaatcg tttcagctga tgatcctaaa gttgctgttt cttttggtat
    13621 tagaaaagta aaacaagaga agatatactt ttttgagggg cgaaaacttg taaaagagct
    13681 tcaaaaaaat ggtttttatt ttaaaatcaa tgaataagta aggagagaaa aaatgatccg
    13741 cacaacaaca aaattgtctg ccaaagaact ggaatctgtt ctatcgattt ggctgcatgc
    13801 taacttggat tctcatgaat tcattccatc aacttattgg gagaatcaag tagaatctgt
    13861 taggaaagct ttaccagatg cagtgattta cttagcagaa atagatcaag aaattattgg
    13921 ttttgctggg gtgattgatc agtttatcgc aggaatcttt gtgaaaaggg aataccggca
    13981 acaaagaatc ggtacgcaac taatgaaaaa aataaaagaa accaataaaa cactgacttt
    14041 gacagtctat caaaaaaatc aatcagctat tcatttctat ctttcacaag gttttacaat
    14101 tcaaacacag caaaaagatg aagcagtaaa tgaggaggaa cttgtgatga aatggaatca
    14161 aaacgctgat ttttgatgaa cagaataacg aaataatgct cccagcctaa ttttttgctg
    14221 ggagcattat ttcattttga agtcactaat ttaactgtgt gaatatgcag ttgttacaat
    14281 ctttgataaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
    14341 tgaggaacta tttttccatt tgtttctcta atactcaggt caaaacgctg atttcacaac
    14401 ctcttgataa acggtgttca aacggctgct tctcggtaat aagcccacta gcctagaaaa
    14461 tatgaaaagc tattttctaa ctagcgttct tattactcaa agcataacgc tgatttcaca
    14521 acctcttgat aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa
    14581 acatgaggaa ctatttttcc gtttctttct ctaatactca ggtcagaacg ctgatttcac
    14641 aacctctctt tcacaacctt attttactcg tttataattt tcatcgaagt attttccgct
    14701 acggatatca tctggcattc catcgtatgg ttcttcgcta ataacatctt cattgtctat
    14761 acctgcatca cccatataag tgatcgtagc aaattcagga acaagtaatt ttggataagt
    14821 ttcatatttt tcacgagctg cttccatttc tgcgatatgt tcgttttgga aagtaacagt
    14881 gatttctgga tgttcttcaa cccatttagg gaaaaagatc attccgtgaa gtttcttttc
    14941 atttggcata aagtccaatg ccacgtctgt ccctgttggc tctgtccaag tgattttgaa
    15001 tacgccgtcc gtcaatttaa cgatatcagc ttcttggtct tttacccaac ggcctttgac
    15061 cattccgcca tggatacggt aatcaacagt atggtcattt ttggcatacc attcatattc
    15121 ccaaccattg tcatacgtat agataaagtg agttccaata aaatcttcca attctttaaa
    15181 ttctttcatg tttaagcatc tccttataaa ttaaaacatg taataaacta catgttttcg
    15241 attactcatt tatgatagaa tggatttaaa gatttgtcaa ggagggaaaa ctgatggcaa
    15301 gaaaaaatac acttcgatat attttgttag ggttattaag taagaagaag atgacaggat
    15361 atgaattgaa tcaatcgttc aaaaatgaaa taggagaatt ttggcaagca aaacatagtc
    15421 agatttatcc tgaacttgca aaaatggaag agcaaggaat cattcagcat caagtagaga
    15481 tcacaggtga aaaattggaa aagaaagttt atgaattaac agaagaaggg aaggaacaat
    15541 tggcagagtg gattcataca cccaccaatg aactaccggt taatcgagac gaatttgttc
    15601 tgaaattgta tttcgtcaaa gacgtgaatg atccggcatt agtagaaatt attcagcagc
    15661 agcgtgacct tcatgaagaa aaacgacttc atctattaga gcgaaaaaaa acgatctttc
    15721 ctactgaaaa agaaaaacat gaaaattacg gacactacct gattttagcg catgcgatca
    15781 atcgggaaac agaatatgca aattggctaa cagatgtatt agaagaaagt aaccgaaaaa
    15841 aataaaaaaa tatccgaaca gtgatggaga attcctttct atatgaaaga aacgcagatc
    15901 accgttcgga tattcttatg ttcaaacagg ccttttttca agtatttatg tagcatctac
    15961 ttattaaaaa gcttggtact taggagatta gtggcgttta gagttctttt aacaactgtt
    16021 cgtagtcagc tttttcatcg acaggaagga gcttgtaagt taattcgtct gtctcgttgt
    16081 catgaatgat gtacatctta gcattctctt ctttgaagtt accgatttgt aaaatcgtat
    16141 agcgttcgtc gatcaattcg atatctttga ttgcttttga aaaaacttta cggacatggt
    16201 gcattttctt ttggcggatt tctgcctttt ctgcagcttt tgacaaagta aatccgtcat
    16261 ctaaatattt tttgatcagt tctactttta tgactgtagg taaacgatat tttctaggac
    16321 aattcgcatc agcttcgatc gaacgaatga atcccttttg ttcccaatac cttaattgac
    16381 gtggagacac acctacgata tcacttaatt cactgatgcc aacaaccaat tgatcatttt
    16441 ccaacaaacg tttaagggaa gccccaacca tttttgtcac tccttttgtc ctcttgcttc
    16501 ccttaattat atccgtatta acttatgtgt caagtaggtt gacaaatagc gaatttagga
    16561 ctaaagtatt ctagtagttt cagaaaaaat tcagaaagaa ggagaaatat gagtaacaac
    16621 caacccgtag atatttacgg aaaaccatat aatcggacac ttttagttgt tgtcttatta
    16681 attggtacat tttgtaccgt tttgaatcag accttattga cgacagcttt tcctacattg
    16741 atgaaagctt tcgatatttc agcgtctgat gtgcaatggt taacgactgg tttcttgcta
    16801 gtcaacggga tcatgatccc aataacagca tggctgatca ataaattcag ttcaagaaat
    16861 ttatatcttt ctgccatgac gatctttttg atcggaacga ttacttgctt tacagcacct
    16921 aattttagta cattactaat cggtcgtttg atccaagctt gcggtgtggg agtatcgatg
    16981 ccattattgc aaaatatcat gctatctatt ttcccgccag aaaaaagagg atcagcaatg
    17041 gggatgtcag ggatcgttat tggacttgct cctgctttag gccctacatt atctggttat
    17101 atcattgata actaccattg gcgtgacttg tttggtatgg tactgccaat cgttgttctt
    17161 gtcttggttt tagcattctt cctaatgaaa agtgttattc cattgtcaaa tcctagcatt
    17221 gatattttat ccgctatttt atcaacgatt ggattcggta gtttacttta cggattttct
    17281 agtgtaggag atgacggctg gggaagcttg aaagtaattg gtttcttagt tgttggtgtg
    17341 attttcatcg ccttgttctc atggcgtcaa ttacatttgg aacatccatt cttggaactt
    17401 cgtgtattca aatcatctac attcacgatt gcagctgttt tagctggtgt aacaaatatg
    17461 gcgatggtag gggcagaaat ggtcgtacca ttgtatattc aaaatattcg tggagaatca
    17521 gcattccatt ctggcttgat gttgttgccg ggtgcattga tcatgggggc tatgatgcct
    17581 gtgacgggtg cgattttcga taaatacggc gcgaaacgtt tagccatcac agggatgttc
    17641 attttgacag cggcaactgc accatttgct ttcttgacaa aaacgactcc agttttatat
    17701 atcgtgatcc tttatgcgat ccgtatgttt gggatctcca tggtcatgat gccggtaacg
    17761 acttctggta tgaatgcatt gccaaataac ttgatcagtc atgggacagc ggtaaataat
    17821 acattccgtc aaatcgcaag ctcgattggt acagctattt tgatcagtgt gttgacgaat
    17881 gtgacaaaag caaatatccc aagcaaatct gtattggata atacgccatt agcttataaa
    17941 gatcaggcaa tcaatgccac actgtcaggt taccatgccg cgtttatcgt agcgatcatc
    18001 tttgggattc ttggctttat tattgccttt ttattgaaga aaaaagaacc tgttctaatg
    18061 aaagcggggg ataatgcatg attattggag ttttgattgt cagcttgctt gcttttgctt
    18121 taacgaacgt gttcgctaaa aaaacatggc aaaccttttt atctttgatt ttcggactaa
    18181 tcttcgtcgc ttcactaagt ttgatcgtcg ctaatttgtc caatcacttt ggaatggaaa
    18241 aagtaacgga aacaaaaaca gaaaaaattg tttccagtgc tgattcccaa ggagcagata
    18301 tgcttttgta taaagcttta gggaatggaa aagaaaaagt gtatctttac cgaacaaatg
    18361 aaaaacaaga aaaaccaaaa gcaactggta cggataacga aacaaataaa gtagaaaaga
    18421 cagacggcga tgctgaaaaa gtgacgaaaa ctacttattg ggaatacaaa aacgacatgt
    18481 ataaattctg gttcaatatt gctgataaca atcacgaata tgacagccga gtgaatacat
    18541 tcaaaatccc agagacatgg gtagaattaa gtacggacca agcagccaag ttagctgaat
    18601 tagtgaagaa acagcaaagt acgatggaaa gtgaagcaaa agcttatgtt caagacggta
    18661 tggtaaaagc aatgactgaa aatccaaaaa tgagcaaagc agagcaagaa caacgtacga
    18721 aagccttagc tgctgaattc caacaacaag cattcgcaaa attagtgaaa gaagcaaaag
    18781 gcgaataata ttttgaacac catgtaggag gttgtgaaaa agctgctctg catcaagcag
    18841 taagaacaat cagataaaaa tagcactcca tatttttcat ctgattgggc ttagtgccgt
    18901 agatgcaagc ttttgaacac cgtttaagga agaggttgtg agcctgccgt ttaactctga
    18961 gtcacaagga gcaatttcaa aaaacagctt ctcatgtttg ttgactattg tgacttgtga
    19021 ccgaggagtt ggcaggagaa caccgtttat tcgaaaaaag gaactgcgac aagacttttg
    19081 tcacagttcc ttcttttttt tgctatgcta tactagatgt aaaaaatagg agaaataaaa
    19141 tgagggaaat ttattctttt gaatcaaaaa tctatgcttc cgaagtcggc aagggcgggg
    19201 catatattat ttttccgtat gatataagaa aagaattcgg tgctggaaga gttaaagtcc
    19261 aagcaacttt tgacaatgtg ccgtatgaag gcagtatcgt gaatatgggc gtgaaaaatc
    19321 cagatggttc agtctgctac atccttggac tacgaaaaga tattcgtaaa aacatcggaa
    19381 aagaaatcgg tgatattgta gcagttacag tgaagcaaaa ataaaagagg ttgtgacaaa
    19441 agacgtgtca caacctcttg ataaacggtg ttcaaaagcc agtttctcgg tagtaagtca
    19501 aatttatgca aaaatgtgga aacccatgtt tacaaaattt ttcttactac tcgaaacgaa
    19561 gcggctttat cacaacctct tgataaacgg cgttcaatca gctgtccctc ggtattagct
    19621 caaaaacggc aaaatatgag aagctatttt tccatttttt tctctcatac tcaggtcata
    19681 acgctgattt cacaacctct tctttcataa tggtttttca ccatctttat aataaactgg
    19741 ttatttgata tggaaatcta ttttttccaa ccaatctaaa atcttgtaac ctaactcttc
    19801 tgacatcgga aaagaggcgt tgatttgtgc attggagata tggtcaacat ggataccgga
    19861 agtgatcgta caacttcccg gaacggcagt tttgatgatc acagctattt tttcggctaa
    19921 gacatcgtct ttatggaacc gaccatcgtg gctagggaaa cgtacagttt gcatctctgt
    19981 atcacgagaa aatgtagtga ctgttccgat atgtggtgta tctccgcctg ttaaagtgat
    20041 caataagtct ttaccgatta ctttaacagc agcttggatc gtgaaaccta gtttggttac
    20101 ttcaactatc gtttcttttt ccatattaat caccctgcca acgttttgaa tgctcattta
    20161 caatttcaaa ttgatccaaa agtttcattg tatggtgatt aacaagatct tggatcgttt
    20221 gaggctgatt ataaaaagca ggaattggtg gtatgatttg cacacctagt cgtgctaatt
    20281 tagtcaagtt ttccaagtga atggcattta atggcgtttc acgaggaaca atgactaact
    20341 ttcgttgctc cttcattgtt acatcagcag cacgtgcaat caggttgtcg cctaaaccaa
    20401 cagagatacc tgcgactgtt ttcatacttg ccggtacgat gatcattcca tcggtcaaaa
    20461 atgatccgct ggcgacagca gcacctaaat ctttgtttga ataatggtaa tcaaacaggc
    20521 tttcgaattc agttagtgat aagtcggatt ccagctttaa gttctgtttt gcccatggac
    20581 tgatgattcc gtgtgtttct atcatcggat attcttttag tttcttcact aggttgatgg
    20641 cataaatggt gcctgaagcg ccacttacac caatcacgat ttttttcttc cgttgtgcca
    20701 attgttccat tttccgcact cctttttatg atgtcaaaac agttgcagat cattcattat
    20761 ttcaaatatt tttcccaatc ttctacttct tggaattttg cacgtacaaa atgttctttc
    20821 atatcgaaag gaactgtacc gtcaaagata gtttttgcac ttatcccacg gaaacgaata
    20881 gagccaggat tatattctgg taattctgat gggtccaatg gatggttcct cattcctggg
    20941 atcacgatga tatctttatc tccttggaaa cgagtgttga aagtccaaac tacatcgttc
    21001 atgtcgaaaa tatcaacatc ttcatcgact aaaatgactg tcttcaattc tttgaaggct
    21061 gataaagcaa gaatagctgc ttgacgctga ataccttcat ccgcttcgtt ttctttacgg
    21121 atttgcatga tggtcatcaa tttaccacca cctgcaggag gattgtagac atttactact
    21181 tttcctggaa tagctttatc gaccaaggca agaatacttg cttctgtagg aataccggcc
    21241 atagatacgt gttcttcact gggaccaatc gttgtttgca tgattggatt gtcttttcgg
    21301 tgagtaactg ctttgacttt cactacttgt acagctggat tagcatcccc agtatagcca
    21361 ggaaattctg gcattgcttt tcctgagttc gtgttgatat cttcttgcat tgtttcattt
    21421 ggcatgattt ctgcttcaat gatgaattca gcacgagcaa tacccacttc atcgactgct
    21481 ataccatcaa cgatttgaat aggttcgtta cgcaaagctc ctgcacacca tagttcattg
    21541 tagccaagtg gagtggtagg tggttcgaaa gtcgtgccaa tggcaattgc tggatctaaa
    21601 ccaatattga tagtgatagg cattggttta ttcaaggctt cgaattgttt ttggaagtga
    21661 ccaatgtggc gtccgccagg catgatgtac ataccgatcg tatctttatc ttctagaacc
    21721 atacgatgga tcgttacatc gctcattgtt ttttctggat ctgatcctaa gactacaccc
    21781 attgtgatgt atggaccagc atcttcagga gtatttgtag gtgcaggcaa aatgttgcga
    21841 atatcgaagt ctgcatcggt agctagatga acgacttctt gtgctggagc gtctgctttg
    21901 tctactttca caggtttgat tggattttct acagcgtctt tcaagaaacg accaagtgtt
    21961 cgataatcat ggtgcaaaat ttcacctaca cgtttacgac tagccattgt tccgatgttc
    22021 acacgtgtgc cggggaaacc tttgatattg ttgaaagtca tagctggtcc ttcttgagtc
    22081 ggacgtttta ctgtaccgcc agcaccgata taacggtaaa caccagataa ttctgcttca
    22141 ggatcgattt caatatctgt ttcgtgatat tgacctggaa gtgtttttag ttcttcgatg
    22201 acttttctta aatcatacgg ttgatctgtc atgagagatt cctccttagt tgtctttacc
    22261 tatctatagt aaaaggtgaa aaagaggaaa acaattcaac ttgttcggta aagtattcta
    22321 caatggaatg gatgcgtttt tttgataata gaataaacta tgatatactt atcactaaaa
    22381 gaaagcgcat acttagtgtt ggaggaagga aagtgaaaaa aatggaggag tcatttccta
    22441 aagcagtaaa agtggaaaat atagcgaata ttttgaaagt tacatttgaa aacggagaag
    22501 taaaatacgt gaaatctcat tggacagaag agataaccga tgccttgcaa tttggaaaaa
    22561 aaggaagagg aaaaagaaaa aatcttctcg cactttctcg taatatgtgg attgggactg
    22621 aagtaacgat tgaagcagat ggaacagtat tcataaatgg aaaagacagg tatacgccag
    22681 aagagttgtg gtacaaagga aagaaaagta taccagaatt ataaaaaata attaaaaaaa
    22741 acagcgcgaa acaaggaaca tattacttcc tcgtttcttg ctgttttcta ctatttcagc
    22801 gctcttatga ctcaaatcta aatgtggttg tcacaacctt attctgcacg caaagcgata
    22861 gcggcgtctt ttttcgctgc cattcgtgca ggaagatgac cgccaagcat cgtcaatata
    22921 gtagagacga tcactagaat aatcgcatgg acaggattta gttgagcaac attttttaga
    22981 tccgtcatgt tgtataagat gctattgata gggaacgtag ctagccatgc aataaacaca
    23041 cctaatatcc cagaagaaat acctaaaata cacgtttctg cgtcaaatac gcgagtgatg
    23101 tcttttttac gtgcacctaa agcttttagt acaccgattt cttttgtccg ttcaatcaca
    23161 gatgtataag taatgatact gatcatgatc atacttgtta ctaaagagat ccctgcaaaa
    23221 gcaataagga cataagtgat agcatccatc aatccgccag tcagttcagt catcgttcca
    23281 gctaaatcag tataaatgat tttatcttca tctgatttgc ctttattgta atcatctaga
    23341 taatccaaga tcttttcttt gtcttcaaaa ttgttcggat aaatcatgat acttgaaggc
    23401 aagctgtctc cacctaaata actaagcaat gtttgtttgg cattttcgtc aactttttca
    23461 gtagttaaga catttacacc actgtctctt tgagctttga cgatttctga ttctttgttt
    23521 tcgttgacga tttgagtcgt taattgatcg ctataagcga ttcctggaga taagagattc
    23581 attgtagaag aagatttcac gcgtaggatc cccgagatct tcaattcatc agaagcattc
    23641 tgatacatcg catcataatc tgtattaggg ataaaattcc cagttggaag ttttgtatag
    23701 taagtattgt tgtttactaa tttgaatgtt gtaccaacga tatcatcaaa atcaagtttt
    23761 tcgtcttctt tcacatcaaa tccaagattc ttcaatgcgt tgatattagt attattattg
    23821 ccgtctacga tcagcacgac atctgttgct gaggctggat aacttccggc aagtaaagaa
    23881 taattgtctt ttaaaaagtt ttctttgcta gtgtcaagct gtgtagggaa acttgacact
    23941 cccacacctg tcattgctga catcgtacta gataaagaaa gagattccgt atcaggattt
    24001 tgattcgaga agctgacagg ctgtactttg ccatttacat cacgtaaaag attgattcct
    24061 gtagtacggg taaatccgat attattactt aagttaggat cgatgtctgt tacgtaatcg
    24121 acgtatgttt gatcaatctt attcgtgtgc tgtgctttat cttcttcgct gacttttgca
    24181 gtaatcgttt tgctgtctgg ataatccgct ttgcttgcac ccaatccggc atcgtcacga
    24241 gtctggttag tcgttacctt agaaatagta atcggaaatt tcgccatcgt ttcagcttgc
    24301 gtgttatcga tttgtttttg gaatccagaa gaaagggaca gaacgatcgc gattccgatg
    24361 atcccaatac tggaagcaaa ggaggtcaag aaagtacgtc ctttttttgt tcggatattg
    24421 ttgaaggaaa gctttaacgc tgtccaaaaa ctcatctttg ttctgcgtaa gttaaattga
    24481 tcatcttttg gtcgttcgat gtgaggatgg gaatctgtta agatcttacc atcagaaaat
    24541 tcaatgattc gatctgcgta ttgatgggcc agttcaggat tatgtgtgac catgatcact
    24601 aatttttctt tcgataactc ttggatcaat tccatgatct ggatacttgt ttctgtatct
    24661 aacgctccgg ttggttcatc gcacaaaagg atatctgggt cattggctag tgctcgagcg
    24721 atggccactc gctgcatttg tccaccagat aactgatttg gctttttatg catatgattt
    24781 gtcaagccga ctcggtgcaa tgcatcttct gcccgttttc gtttttcgtc tttagaaaca
    24841 ccacttaatg tcatcccaag ttcaacgttt tcaataatgc ctagatgacc aatcaagttg
    24901 taactttgaa aaatgaaacc gatagagttg tttcggtagg catcccagtc actgtcctta
    24961 aagtctttag tagactttcc gttgattacc atatcaccgg aatcataatt atctagtcca
    25021 ccaatcacat tcagcatagt cgttttacca gaaccactag gacctaaaat cgctacgaat
    25081 tctttttgtc tgaaagcgac agacacacca tccaatgctt tggtggtggt ttcgccgact
    25141 ttataatatt tttttatatc ttttaattga agcataatca cccttctccc gtattgaaat
    25201 attcggtata ctaataagta tatcagaaca atgatcgtag tatacgaaat aaatgttaac
    25261 tgaagatgaa ctacaagaaa aaatctagaa atttttcaaa aaacagaggg agatcaatga
    25321 atcgtatttt agtagtagaa gatgatccgc aattacagtt gctttaccgt tctgtgttgg
    25381 aacgagcggg cttttcagtc atcaccgcat tgaatggaga ggatgcgctg aaaaaactag
    25441 aagcgacacg tatagaatta atcattacag atatcatgat gccgaaaatg gatggctatg
    25501 acttgctaga ctcattacgt agcaccagat ttgacacacc tgttctaatg attacggcaa
    25561 aggccgattt tgaggataag aaacgcgggt tcaaattagg agcagatgat tatatgacaa
    25621 aacccatcga tgtcaatgag atgatcctac gtgtggaagc attgcttcga cgttcaaaga
    25681 tcaattacag cagtagcttg ataatcggac aaacaaaatt agagctagat gcttatacag
    25741 tgttacaaga tcagcattcg ttgattctgc ctcaaaaaga atttctgctt ctgtataaat
    25801 tactgtctta tcccaacaag atcttcactc gccaacagtt gatggatgat atttggggat
    25861 tagatacgaa tacagaagaa cgaacgatcg atgtacatat caaacgttta aggacccgtt
    25921 ttgaaaagac ggaagatttt caaatcatca cagtacgggg actaggctat aaggcggtta
    25981 tttaaagatg aaaaaaaagc tattttcaca attatggatc tattttgcta gtattgtctt
    26041 catttcgatc ttagtgacac tgctgtgttt tctcggtttt attttcttat tgtctcgaca
    26101 ttctatctcg cctgctgaat cggctggaaa accaacgtta tacccgttat tcgtctttgc
    26161 ggggttcagt atggtcgtgg gtacaggaat atcgattttt gttgggcggc gtatcctgca
    26221 tccaatcagt gcgctaggga caaatatgag tctggtagca acgggagatt tttcgattcg
    26281 aatggatgaa cagcagaaag tagcagaagt acagcaactg tataaagatt tcaacgttat
    26341 ggtccaagaa ctcaatagta tcgagacact gaggaatgac tttgtgtcca gtgtatccca
    26401 tgaattcaaa acaccactag caacaatcca aggatatgtt caattgttac aagccccaaa
    26461 tctctctgac gaagaacgcc aaatattttt atatcggatt atcgaaagta tcacgcagct
    26521 ttcacaatta acagagaaca tcttaaaatt gaacaaactg gaaaaccagc gtatccaact
    26581 ggaaaagaaa gagtatcgat tagatgaaca aatcagagaa gtaatcgttt tttttcagcc
    26641 taaatgggag aaagaacagc tagaattgga tatcgaactt gctgcagtaa attacacagg
    26701 caatgaagag tttttgtacc aagtgtggct gaatatcatg gataacgcca taaagtacaa
    26761 tcaaatcaat ggacagattc atatcaaact atttgagaca gccacagaga ttgtactgga
    26821 agtgaccgat tcaggtgtag gaatgaatga agagaccaga gatcgaatgt ttgaaaaatt
    26881 ttatcaagga gatacaagtc gtcaaatttc tgggaatggg ctaggacttt cactggttaa
    26941 aaaaattcta gaactgcatg atggacgtat tgattatagc agtatcgaag gagtaggaac
    27001 gacagcaatg atacggttaa gtaaacaata ggaaaaagaa taaaacccct aaaaattcta
    27061 ttgaaggttt ttaggggttt ttagtcgttt ttttgtgatt agggaaatgt tcttacggtt
    27121 cttttggcat aaaaatcgct gctttcttat tgaactacga gaaatgttag aggtgtcatt
    27181 gaaacaagga catgatgcaa gaaagagagc gaaagtcatg cgtcgctctc tttttttggg
    27241 tggatagatg gacagaaaat gtagggctta gttttcttct tgatttaaat actcccaaaa
    27301 tgtatctgaa gccaaagaat gctcaccgac tttacgagta aatgcaagat aactctctgt
    27361 tgttggagaa atagggagaa tcacagtatt ttcttttaat agattcttga ctgttttttc
    27421 cataacaata gcaatcccca tgtcatttgc aatcatattg atgatcaagt cgattcttgc
    27481 tcctttataa gagatttttg gttcaaatcc agcttcgcga cacagatcta gtacaggttg
    27541 atacaagttc gtagtggggc caaggagcaa aaagttctcc tttttcagtt cgcttagatc
    27601 caatttcttt ttactagcta aaggatgatt tttaggtaga actgctacaa aatcgtctgt
    27661 ttcagtaggc aaccactcaa tcgaacttgg tggggcctgg aaacttctag caaagtggat
    27721 cgtgtcacta tgttcattgg cgtcattgta agttaattcg acactttcca cttctttcaa
    27781 ctggactgta aattcaggat ggcgtttcaa aaactcggtg attttggaaa aacctttata
    27841 attgatcatt gtcggtatcg ttaagatatt taatgaaaga ttgttgtctt tcgtataggt
    27901 ttcaagcact tcttgcatag catggtattg ttctacgaca ttttttacat atgggagcaa
    27961 taagcttcca gcttcggtca attcgatttt tctatgggcg cgttcgaata atttgacgtc
    28021 taattctgtt tctaacgaaa gaatctgttt agaaatattt ccttgggtgg tgaattgtcg
    28081 atcagcagtt ttggtgaaat tcattgtttc ggctaaatca agaaaaactt cgaatttatg
    28141 gatatccatc atattcctcc tacttattcc aataaagaat actttaacac aaaaaatgaa
    28201 ttggtgtaat cttctctgtc tgttacaatc tttcttgcag cgaaagcaat aattacagaa
    28261 atttttctga tttttttcgc ttcgtagaaa aagagaggag ttttttgatg ccaactggag
    28321 ttttgatcaa tgtaggttcc gttttgtttg gcggactaat tggcggatta gtaggaaata
    28381 aattaagtga acattttaaa gcacagctga cgatggtttt tggtgtgtgt tcgatgggga
    28441 tgggaatcta ttccattgcg ccgatgaaaa atatgccggc tgttattttt gctttagtga
    28501 taggaacagc gattggtttg attgttcatt taggtaatgg gataaataag ggggctgcgc
    28561 tgatgcaagt acctatttct aaaatcttcc ctagtgaaaa gcttggtatg acccacgatg
    28621 aatttatcag cacattagtg acggtcattg ttctattttg tgcaagtggt acggggatct
    28681 atggaagttt agattctggg atgacaggag acagtacgat tttgatttct aaatctgtcc
    28741 ttgatttttt cacagcagct atttttgcgt gtaatttagg gtatgtcgtt tctgtgatag
    28801 caattcccca attcattatt ttttatattt tgttcttatt ggcaaaattt atttatccat
    28861 tgactacacc agacatgatc ttagatttta aagcttgcgg cggattcttg atggtcgcta
    28921 ccggttttag aatgatcaac gtgaaaatgt tcccagtagc tgacatgata cctgcaatga
    28981 tcgttatcat gccactgagc tggatgtgga caaattggat tatgccactg ttataataaa
    29041 aatcaaagag aagccatttt ttgctgacta aaaaggatca aatcttttag tcagtttttt
    29101 tgtatttgac aaactgattt tgaaactata ttatatgttg tataatatag attgaaataa
    29161 aatattacac ttgagaatag aaggtgacgt aatgcacatc cagattccaa ctgttcttct
    29221 tgatggtact gtgctagcag cattaagcaa ggaagatatg tatgggtatg ccttaacaaa
    29281 aatcgtacaa gaaagtctct ctgtcagtga atctacacta tatccggttt tgagaagatt
    29341 gaaaaaagaa ggatatcttg agacctatga tgaacctttt gaaggcagaa accgtcgata
    29401 ttactcgttg acaccaatgg gaagtcgtca tttagaagaa attattgatg aatggattgt
    29461 ttttcgagat tctttagacc ggttattgga ggagagcaca aatgaatgaa tatttgaatg
    29521 aagttgtgga tcatcttcaa gaaattcctg aagacgatcg ttttgatttg atccagtatt
    29581 atgaggaata ttttttagat tctggaaaaa cattagatca gatcattgat gaatatggta
    29641 caccaaagca gtttgcctta aaattaaaaa tcagttactt ttcagaagaa gaacaagcag
    29701 aaaacgagtt agtgaaaaat ccaaaaagac aaattcgcct gatttggtta atcattatcg
    29761 gtttatttgc ttctcctgtc ctcattccac tagcactggg gtttatattg gttattgggg
    29821 gactattggt tgctttacta gcgctcattt ttagtatcta tgttttatgt atcagtatct
    29881 taggtgttgg cctattcatg atcatctcag gaatcagtgt gtttggacaa tcgattctga
    29941 gtggattatt ctttatcggt gtcggtctgt tcgccacagg aagcgcagta tttatttcgc
    30001 cactgttgct gaaaggaaca aaagggcttt tttatgtcat gatgaacttt gttaaatgga
    30061 tgggacgacg ttttattaca aaaagagatc tgcattcagc gggcgtttga aggaggacac
    30121 ataaattgag attgaaacat tttttgatta ccggtattgg tctgatgctt actggtggcc
    30181 tcctagcagg tgcaacttat acattaggcg ctcaaaagag tctgacttgg gataatggtc
    30241 ctcggttgat cgatatggtt actgagacaa aaaaaatcaa ggatgcaaac aaaatcgtca
    30301 tccatgcaga aaatcaaaca gtttcggtca tacgcggatt ggattttgag ataaaaacga
    30361 cttatgataa gacagaaaag ccagaaataa aggagaaaaa tggcgtgttg actgtgaatg
    30421 cagtcaaaaa atcaacaggt gcaatcatta gtttagaaga cacaagtgca caaatcacta
    30481 ttgttgtccc tgcagatcat gaattatctg aattgacaat cgaagggaat aatagtgcaa
    30541 ttgatatcaa cgaagtatca atcaaaaaag tgaatactaa gacacatgat ggctgggtat
    30601 cgttcaatga atttacagga ggaacaataa ctgcgaaaga tgaattaggc tcacttcgat
    30661 tagatgaggc caatgtggat aacttgatcg tcacaggtga aaacagcaac ttccatctag
    30721 aagacagttt gatcagcaaa gaaggaaaag tcatcctaaa cggtggcaat ttatctttgc
    30781 atgagatgga aaacagtggc tatactatcc aaacgtctgg tgaatcttat attgaaaaag
    30841 actaccagcc aataacaaat actttgttag aacaaggaca gaaaaagcta aagatcactg
    30901 ctaaagacac atcgatcaat attacgacgg atcaagagga caatgaaaca gattgatcct
    30961 gtactcaact ggatatttta agaaaaaaca gcaaagatcc ttccaatctt tgctgttttt
    31021 catcgcttaa cgcacataac tatagtaaaa taagaaagaa aatatctaaa aagaaaacag
    31081 ggtgaaagag tatatgaagg tagaagaagg tcaaagagaa aagttaaaaa cagaaatgga
    31141 aagattacat acttatatca ctcaactaag ccaaaccttc tatgatccag acaaagaaaa
    31201 agtgatggtt aattacccga acaattcaga aggtcgccaa ttagagcaag tgtaccatga
    31261 agttttcaaa catttattga cggtgaagaa agagctggat tattattctt taccgattat
    31321 cgatacagga atattgaagt atgatgggaa gaaggaacga tttatcttta aatctgttcg
    31381 ggaagatttg cctctttcag cagggatgga tctagaggtc ttggtagaag actattttac
    31441 agaagaaaag cactgggttc gaacaaaatt ggattattta ccgcaggcag ccggagggac
    31501 acaggcaagc ggttggtaca tcactgaaga caaagagtta gaactagaag gtgtaatggc
    31561 gcgtattcga aaaaaaaatt aaaaaagtaa cattagatat ttattgaagg ataaaggagc
    31621 gcaggaagta gcatgagaga aaaaagagca ttttatatca atggttatgc agggattgtc
    31681 ggattggtcg tattagcttt agttggattg tttttcttct atttaggtat gtggcaagcg
    31741 aaagtttggg cacttttttt aagtattttt ctttggttga tcacgttatt gttgttaagt
    31801 tcggcaactg tagtcagtcc taatcaagca aaagtaattt tgttttttgg acaatattta
    31861 ggtacgattc gagaaaatgg ctttttcctt acgatacctt tggcacaaaa aatgactgtt
    31921 tcgttgaaag ttcggaattt caatagttct gttttaaaag tcaatgattt ggatggaaac
    31981 cccatcgaaa tctcagcagt agtagtcttt aaagtcgttg atacagcaaa ggcattattc
    32041 gatgtagcgt attatcaaga ttttgtagaa atccaaagtg agacagccat tcggcatatc
    32101 gctagccaat acccgtatga tacattcaat gagaatgatt tgacgcttcg aggaaacacg
    32161 acggcagtgt ctgacgagtt gcaaaaagag ctgcaagagc gtttagcagt agcaggtgtc
    32221 gaagtaatcg aaacacgctt gaaccattta gcctatgcaa cagaaatcgc tagtgccatg
    32281 ctccaacgtc aacaagcaaa agccattttg tctgctcgac aaaccattgt tgaaggagct
    32341 gtcacaatca ctcaaatggc cttagagcaa atcgaagatg gacaagaaat caatttcacc
    32401 gatgatcgga aagtccaatt gatcaataat ttacttgtct caatcattac agacaaagga
    32461 acgcaacctg ttatcaatac aggcgacatg aaagaccgta agtgacatac tttttgtaag
    32521 ataggaagaa ggagtttagg acggattatt gtcttgaact ctttttttag ttgttttaaa
    32581 gaaaatgaca aaattgaaag aagaatgata gttgattcga aggcagaatt gttcgctttt
    32641 ttctataatc agtatataaa gtaagagaga aggggaaaaa gtgaaaaaac ttctgatcgg
    32701 tctacttgtt ttcatcgttg tggtacttgc aggggtgaaa atcgcagatt acgtagttat
    32761 gggtggagaa gaatactatg tacaaattac gacagatggt acaaaaacag tttcaaaatc
    32821 agactctgga caagaaacag tcaactatag atatatcctg cctggctata cgaaagaggg
    32881 taaggaaaaa gaactggact tcaagggaca attgactcga ccgcttcgta aaggagcata
    32941 tttaaaaatc acctggaaca aaaataaagg tgtcacttcg tatgaagaag tcgaaaaaaa
    33001 agacgtgccg aaaacagcac tggaaaaact aattaaaggg tagggaaaga aatgaacaaa
    33061 atagtcgaag tacagaatgt aacaaaagta tacggaaaaa acaatgagaa aaagacgcaa
    33121 gcattaagcg gcatcagctt cgatgtagaa aaaggcgaat ttattgggat catgggcgct
    33181 tctggttctg gtaagtcgac acttttgaat attttatcca cattagataa accaacagat
    33241 gggcatatcc gaatcaatca aaaagatgtg accacattaa aaggaaatca actagcagat
    33301 tttcgtgcga atgagattgg ctttattttc caagatttca atttattaga aagcttgaca
    33361 gcacaagaaa atatcgctgt tcctctttct ttacaaggcg tcagaccaaa agaaatcaag
    33421 caacgtgtcc aaaaaattgc agaacgttta tccatctctc atattttgga aagctatcct
    33481 tctgagattt ccggtggaca aaaacaacgg gtcgccgccg cacgtgcatt gatcacacaa
    33541 ccaacgattc tattagggga tgagcctaca ggtgcgctgg attccaaaag tgcccgggat
    33601 cttttagata cgatggacga attgaataca aaagatcatg tatcgattct tcttgtgacg
    33661 catgatccgt tttctgcaag ttattgccaa cgtattttat tcatcaagga tggcggtatc
    33721 catcaagaag tgaaacgtgg agatcaaagc cgtgattcct tctatcgaga aatactatct
    33781 attcttggaa acttggaaca gtagggggag attgactatg ctttggaaat tatcacttac
    33841 tgggatcaag agccgtttaa gagactactt ggtgttattc tcaggattag ttatggcatc
    33901 tgctattttt tacatgtttg aatcaatggc aagcaacgaa gcttttttga aaagtaatac
    33961 gatcgttgcg gctacagtta tcattttccg attcggttct gttttactag ggatcatcac
    34021 attcgtttat attttatatg caaattcctt tttgatgacg atgcggcaaa aagactatgc
    34081 aatgtttatg atgctgggag caaaaggcag aaaaatcgca cagatgattt tttctgaaac
    34141 attcttagta gggatcgcag ctactttagt gggttcagct gtcggtgtag gattgacaag
    34201 tatcgtccac cgattattag tggatcagtt agatctcaat atttcgcatt ttacaccttt
    34261 tagtattcac gggttgttga tcactgtttt gttttttgct gttttgtttt tattagctgc
    34321 tgttgtcaat gctttttcga ttgtcaaaaa accgatttta gcattgattc gtgcggatca
    34381 gacaccaaca agaatcaagc agcataaatt cttgtttttc cttgaagtag tactgggaat
    34441 tgcttttcta ggctgcggtt actatatgat gaaacacgtg gggacctatc aagtctttgg
    34501 tataggggta gccttagtta cgatcgtatt aggaacttat ttcattttcc attctgtgat
    34561 cattttcttt ttatcattac taaagaaatc agaacagatt tcattgaaaa aattgaacaa
    34621 tttcacattg tcacagttaa actttcgtat tcgtgaatat acacaaatgc tctcaatggt
    34681 cgctatgctg tttgccttgg cactgggcgc tttgacggta ggattaggtt ttaaaaatga
    34741 aattgcagac ttgacagaaa acaattctgg atatgatcta gtgatgaata atcctcgggc
    34801 agaagatcag caaaaaatca aagaactttc accaacactt tctgcttctt atactcaaaa
    34861 agaagatgcg ggaacgattt actataatgc aagtgaattt gcggctcaac cattgatcat
    34921 gataaaacat gaagatggaa caccgccgaa gatcacgaaa gaaaaagttc ctgctaaaaa
    34981 aatgaatgaa ctgactgtac aagaggaact tcgaagctat gaattgccgg aacagcaaga
    35041 aaaagaaatc aaggtagtca gtcaatcaga atttgatcaa ctgaacttgc cacaatccac
    35101 tttacagcta gttcaggtaa aagacttcca agcaaacctg aaaccaatcg aaacacttgt
    35161 tgctcaaaat aaaatgaaca atccatcttt gcaaaaagta gagtacagca accagaaata
    35221 tgaaatgtat gaagtgtata atggtctatt ctctggtttt gaatttatgg gattcttcct
    35281 aggtttagct ttcttgacca tgcttgctag ctgtttgatg ttcaagatct tgtcaggaag
    35341 caagagtgac attgctcgtt atgaaatgct tgaaaagata ggaacacgac gcgggttgct
    35401 taaacaatcg atccgtagag aagtaggcgt gttattctta gcaccaggaa tcttaggtgt
    35461 agtccatgtc ttattcggat tacaaatgtt tggtctattc atgcaaaatc catacaaaga
    35521 tatttgggtt ccgtttacga tattcttcgt attgtatttc atctactatg tactgacgac
    35581 atggttatat actggaatcg tattgaaaaa gagattgtga aaaagctgtc ctgcatcaag
    35641 taataagaac agccaagcaa aaatagctcc tcatattttt cgtttggttg ggcttattac
    35701 cgcagatgca agcttttgaa caccgtttgt tcggaaaaag aggttgtgac aatatttttg
    35761 tcacaacctt tttttcacgg tcttttatta gaatatagta aattttttct cgttactcac
    35821 ggaataagct gttaagaaaa cggtttccat gtatactgga atcagtgcaa atgaaagaaa
    35881 agagggtgca aggaacatgg aaatcaatga acaaaatttt ggcgaaaact atcacttgat
    35941 tacgatcacg aatcaaaatg ggacatcttt gtctgtcagt gatcttgggg cacgggttgt
    36001 tcattttcaa acaataatcg ataatgaaaa acgagagttg atcttaggtt ttgatgaagc
    36061 aaaagaatat ctagaaaaag acccttatat cggtgcttca atcggacgta tggctggacg
    36121 gattacgaat ggcacattca aattagatga tgaaacgttc gaagtaaaaa cagacccgga
    36181 cactggtcat tgtttacatg gtggtgcacc tagctttgaa gccaagcagt ggcattatac
    36241 agtcatcaat ggtgaaaacg aagcatcggt cattttctat cagactagcc cagatatgga
    36301 aaacggattt cctggaactt tagatgtaga agtacggtat acgctgacga acgatgatac
    36361 atggcgagtg acggtccaag ggatcagtga ccgcaaaaca gtattcaatc ctacaaatca
    36421 tgtctatttc aatttaaccg gtgatccagc acagtcaatc gacgaacata tactttggtt
    36481 gaatagccga ttatttgcta cgctcaataa agatacaacg cctacaggag aagtagtttc
    36541 agtaagtggc acagcatttg acttccaacg tcctaaaaaa ttaggtgagg tctttgccag
    36601 tgattttcgc caatcaatga tagtgggcgg catcgatcat ccatttttgt tgaaaaaaca
    36661 atctggttta ctaaatgctg cggaattgac aagcccagat gaaaaagtat cgatccaagt
    36721 aaaaacagat gcttcaggaa tcgtgatttt tacagcaaat tttggagaag atggaccgga
    36781 aatgcgcggg aataaattag taaatcatgg cggtatcaca tttgagactc aagaactgcc
    36841 aggagcggag cgacttgaaa gttttggaga tgtcactctg gaacctaacc aagtacgcga
    36901 gtttgtaacg gaatacaaaa taaat
//
LOCUS       DAJSKS010000009        35400 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJSKS010000009 DAJSKS010000000
VERSION     DAJSKS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.009"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..1128)
                     /locus_tag="ON396_000439"
     CDS             complement(<1..1128)
                     /locus_tag="ON396_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HCR3112573.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYK"
     gene            complement(1225..1725)
                     /locus_tag="ON396_000440"
     CDS             complement(1225..1725)
                     /locus_tag="ON396_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR3112574.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            complement(1738..1959)
                     /locus_tag="ON396_000441"
     CDS             complement(1738..1959)
                     /locus_tag="ON396_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112575.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            complement(1964..2101)
                     /locus_tag="ON396_000442"
     CDS             complement(1964..2101)
                     /locus_tag="ON396_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCR3112576.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(2098..3282)
                     /locus_tag="ON396_000443"
     CDS             complement(2098..3282)
                     /locus_tag="ON396_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR3112577.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(3538..3792)
                     /locus_tag="ON396_000444"
     CDS             complement(3538..3792)
                     /locus_tag="ON396_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112578.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(3817..4959)
                     /locus_tag="ON396_000445"
     CDS             complement(3817..4959)
                     /locus_tag="ON396_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HCR3112579.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(5019..6371)
                     /locus_tag="ON396_000446"
     CDS             complement(5019..6371)
                     /locus_tag="ON396_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR3112580.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(6442..6786)
                     /locus_tag="ON396_000447"
     CDS             complement(6442..6786)
                     /locus_tag="ON396_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR3112581.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(6796..7170)
                     /locus_tag="ON396_000448"
     CDS             complement(6796..7170)
                     /locus_tag="ON396_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR3112582.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(7183..7497)
                     /locus_tag="ON396_000449"
     CDS             complement(7183..7497)
                     /locus_tag="ON396_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR3112583.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(7611..10838)
                     /gene="fss3"
                     /locus_tag="ON396_000450"
     CDS             complement(7611..10838)
                     /gene="fss3"
                     /locus_tag="ON396_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HCR3112584.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            complement(10917..11579)
                     /locus_tag="ON396_000451"
     CDS             complement(10917..11579)
                     /locus_tag="ON396_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112585.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            12196..12426
                     /locus_tag="ON396_000452"
     CDS             12196..12426
                     /locus_tag="ON396_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112586.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            12432..12731
                     /locus_tag="ON396_000453"
     CDS             12432..12731
                     /locus_tag="ON396_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112587.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(13244..13317)
                     /locus_tag="ON396_000454"
     tRNA            complement(13244..13317)
                     /locus_tag="ON396_000454"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13281..13283),aa:Ile,seq:cat)
     gene            complement(13506..14525)
                     /locus_tag="ON396_000455"
     CDS             complement(13506..14525)
                     /locus_tag="ON396_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HCR3112588.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(14522..14746)
                     /locus_tag="ON396_000456"
     CDS             complement(14522..14746)
                     /locus_tag="ON396_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCR3112589.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(14743..15036)
                     /locus_tag="ON396_000457"
     CDS             complement(14743..15036)
                     /locus_tag="ON396_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112590.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            complement(15073..15210)
                     /locus_tag="ON396_000458"
     CDS             complement(15073..15210)
                     /locus_tag="ON396_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCR3112591.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(15212..15643)
                     /locus_tag="ON396_000459"
     CDS             complement(15212..15643)
                     /locus_tag="ON396_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112592.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            complement(15657..16151)
                     /locus_tag="ON396_000460"
     CDS             complement(15657..16151)
                     /locus_tag="ON396_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112593.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            complement(16151..16600)
                     /locus_tag="ON396_000461"
     CDS             complement(16151..16600)
                     /locus_tag="ON396_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112594.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            complement(16604..17221)
                     /locus_tag="ON396_000462"
     CDS             complement(16604..17221)
                     /locus_tag="ON396_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112595.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            complement(17221..18129)
                     /locus_tag="ON396_000463"
     CDS             complement(17221..18129)
                     /locus_tag="ON396_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HCR3112596.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            complement(18142..20901)
                     /locus_tag="ON396_000464"
     CDS             complement(18142..20901)
                     /locus_tag="ON396_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3112597.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLFN
                     AAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYER
                     PAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSG
                     YLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINW
                     ARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISSI
                     VIPPK"
     gene            complement(20898..21602)
                     /locus_tag="ON396_000465"
     CDS             complement(20898..21602)
                     /locus_tag="ON396_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3112598.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(21614..23923)
                     /locus_tag="ON396_000466"
     CDS             complement(21614..23923)
                     /locus_tag="ON396_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR3112599.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(24118..24582)
                     /locus_tag="ON396_000467"
     CDS             complement(24118..24582)
                     /locus_tag="ON396_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112600.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(24582..25208)
                     /locus_tag="ON396_000468"
     CDS             complement(24582..25208)
                     /locus_tag="ON396_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3112601.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(25215..25580)
                     /locus_tag="ON396_000469"
     CDS             complement(25215..25580)
                     /locus_tag="ON396_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCR3112602.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(25570..25908)
                     /locus_tag="ON396_000470"
     CDS             complement(25570..25908)
                     /locus_tag="ON396_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112603.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(25898..26224)
                     /locus_tag="ON396_000471"
     CDS             complement(25898..26224)
                     /locus_tag="ON396_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR3112604.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(26202..26483)
                     /locus_tag="ON396_000472"
     CDS             complement(26202..26483)
                     /locus_tag="ON396_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR3112605.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            complement(26485..27849)
                     /locus_tag="ON396_000473"
     CDS             complement(26485..27849)
                     /locus_tag="ON396_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR3112606.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(27862..28431)
                     /locus_tag="ON396_000474"
     CDS             complement(27862..28431)
                     /locus_tag="ON396_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR3112607.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(28403..29590)
                     /locus_tag="ON396_000475"
     CDS             complement(28403..29590)
                     /locus_tag="ON396_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR3112608.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(29654..31381)
                     /locus_tag="ON396_000476"
     CDS             complement(29654..31381)
                     /locus_tag="ON396_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR3112609.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(31378..31830)
                     /locus_tag="ON396_000477"
     CDS             complement(31378..31830)
                     /locus_tag="ON396_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR3112610.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(31942..32322)
                     /locus_tag="ON396_000478"
     CDS             complement(31942..32322)
                     /locus_tag="ON396_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR3112611.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(32319..32705)
                     /locus_tag="ON396_000479"
     CDS             complement(32319..32705)
                     /locus_tag="ON396_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112612.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(32707..32985)
                     /locus_tag="ON396_000480"
     CDS             complement(32707..32985)
                     /locus_tag="ON396_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112613.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            33033..33761
                     /locus_tag="ON396_000481"
     CDS             33033..33761
                     /locus_tag="ON396_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HCR3112614.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(33907..34383)
                     /locus_tag="ON396_000482"
     CDS             complement(33907..34383)
                     /locus_tag="ON396_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HCR3112615.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(34343..34690)
                     /locus_tag="ON396_000483"
     CDS             complement(34343..34690)
                     /locus_tag="ON396_000483"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112616.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(34677..34862)
                     /locus_tag="ON396_000484"
     CDS             complement(34677..34862)
                     /locus_tag="ON396_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112617.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(34863..35135)
                     /locus_tag="ON396_000485"
     CDS             complement(34863..35135)
                     /locus_tag="ON396_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HCR3112618.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(35132..35335)
                     /locus_tag="ON396_000486"
     CDS             complement(35132..35335)
                     /locus_tag="ON396_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112619.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
BASE COUNT         9998 a         7619 c         5612 g        12171 t
ORIGIN      
        1 cttatagaaa atattcagtt tcacaattgt atccttcaaa aatattccag agccattatg
       61 ttcaatttgt gcaaagttct gataatcact ttgtatttca gaaaattgat caagttctgc
      121 ttcctttcca aggacataga ccaagaatct tgtaatacaa gatttgcctg ttcctcttcc
      181 tccaataatt gaagttaaat tatcgctgaa ttcgactaca acttcttttg tgctaagagt
      241 ggtcccagaa aagcttaatt tttccaataa aacttcattt tcattaatgg agaaattaga
      301 aaaatcagaa tgaactcgag tttcgcctag cattaaagca tcgcgaagtg atcccaagtc
      361 aggttcgtcc ttcattttta tataggaata tcttgtgcct attccccaaa gtccatgttt
      421 tgaatttcct ttcgagtgtg ggttatcaga aaagctaaga aatgtcatat tatttttttc
      481 tgcctcaaca actgatttat accaagattc caaagtatct tttccaactc taccatcata
      541 tctggcattc acactatcat agatagtgtc acgcttagat gagggaatca tttgtgagcg
      601 attttcaaaa aattcttttt gaaccgtctg tactgcatat atattttctg attcaaatat
      661 ctttcctaga tttccaaatt ttgctgaaga aattccatta aactcatcta tatgtgcagg
      721 aataacgagc aagccctcgt gctcagcaaa atcaataact tctaaaactg atttcattga
      781 gcaagctgca gcgctggccc aatcctctct ttttataccc atcttggtta agaaatcaga
      841 gatatcattt gtacatgcac ttttatcaaa taggactagt aaatgagttc cattttctcc
      901 acacgttaat tccactccag gaaaaaaatc taagccattt tcgatagcca ctttcttata
      961 ttcatcaata ttttcccctg tattgtgatc agttaatgca acacaatcga gtcctttgtt
     1021 taaacattct tctacccatt ccttagcagt aaccttttta tcctgaaagc actgactcgc
     1081 agttgaatga atatgaagat cacatttgta ccacttattg ccaaccatta gtaacacccc
     1141 tatcaacaaa tattttctat ttcagtatat caaatagaaa agtaaaagta cgatagaaat
     1201 ttctggtcaa ttctaacaaa tgatttacat tagttcaaaa acccctcgac tcgtaaccaa
     1261 ataattcccg ccaatctcca agtctcgacc acacgcatca aaatcaatat acgtcttcaa
     1321 gtgttctggt acttcgccta aatcaccagc ttctacaatc aagtaatacg ctacatcact
     1381 catgtcatta caatcgggat aatagatgat atcatccaca tgctctacca tttcctcgaa
     1441 agaattgaag aaggcatgtt gaatctctga agcgacctca ccaattggcg ttcctttaag
     1501 ctcttccgct aaaccacaca accgattaat ttcctcaatt ggtgtatact catcaatctc
     1561 aaaaggcagt tcataatcat gaattgcgta ctcttcatat tcgtcattta atccaatccg
     1621 ttctttcact tcatcaaaat cgacgggtgg ggtaaaccat gcccccacca actcaccttc
     1681 attgtatttt ccaaggttcg caatataaac acgtatctgt tccattgtga ttccccctca
     1741 ggcaccgaca attcggttaa acaaattgag taagacatcc ttcacaccag aagcattaaa
     1801 gaccagacct accgcaatca aagcaatcac caagaagcca atcagtttgg aaaactcgcg
     1861 tttaaatcct aaaaacaaac caatgaccac aatcgctagt aagactaaag attgagcatt
     1921 ggttaaaaac cattgataca aattttgacc gaaattcatt tcatcacttt cctttctttt
     1981 ccattgctcg gtccgccgct gcacttgcat gcatcaagca catcacagta acaccaagca
     2041 tactgccacc aaacaccaat aaaatatctt tcacaatttc catcacgatt cctcttttca
     2101 tataatcaaa ttttcaattg ccatactttg ttgctccaaa atctttttgt gccggtctga
     2161 tagtcgagta ttatttacca tatcctgaat gtacgttgtc tgattcaacc gatccaactc
     2221 cttggccatc ttccacgtag gggcgacttg tcgtccaagc cagttcaacg ttcgttcaat
     2281 ggtataaggc ttcggttcag aagttaattg aatttccttc tgattccgtc caatgaagcg
     2341 cccccattgt tcattcaagg gccagttagt tcgccttttg ccttccacct tatccgcaaa
     2401 tcgcaagtac cggttaataa tggaaaatgt ggtactttca atactgcgac cttttaacaa
     2461 atcttgaatt gcatgataag ctcgatcatt tttcagtcgg atttcaaaac gatttttcgt
     2521 ttcggtctgg tctaaaggaa tccctaactt gatatattgc tcatagtctt tttcgtacaa
     2581 acagaaatag acctcactct taagactgcc aatatacaaa gtattgccca taccatcttt
     2641 atcatcaggt ttcaacagtt ccccggaacg atagcttttg aaggtacgaa acaaggagat
     2701 acattcttcc ttctgacatt ttttcgttaa atcaggaata tctaacaggc caactcgatc
     2761 attgattgcc aggtctaagc gtttcatcac gccaccggct tttagacaat cttcgaaaaa
     2821 gtcataccag ctacgctttt gggcgagtaa aaaagtttca aactgccgac aaccacgacc
     2881 ctttaattct aaaaggacgc ctttatcttc ttcatgtgac aacatcacca caatatctcc
     2941 cataacatat tgttcttgat aggagtaaaa ggcgtaatct tcgtggagca tataatcaaa
     3001 tttcaaatgg agaatctctt gaatgatttt cctcgcatcc gttgtgggaa agcgaatcct
     3061 tacatagtca aacagtaacg ttaaaggtaa ctcgggattg aaactttcca gttggtcaaa
     3121 aattttcctc atggtttttg tcgatgcggt tacgccacca ttttctatct gatttaagta
     3181 cgggcgacta agtccacaag tcgtagctaa tttattttgt gaaacgccat attcattacg
     3241 ccggtctttt aatagccgat aatctaaatt cctttgtgcc aagttctcac ctccaaattt
     3301 aaggtgttaa ctttttcaaa tcagttaaca tctacacaaa ttgcttatta tccttgatag
     3361 gacagccatt ccttatcgtt gcttgcttat tttttgtgtt tttgtacccc cctgttagat
     3421 acggggggtg agacatcatg gccggctaac gccgtccata aacaggcaca gcaccacgga
     3481 ggatttctgc ttcgcaaatc ccccgcggtc agtgcctatc agtggtttat ttggccgtta
     3541 ctccgcggta ataccctcaa tttccaactc tgcttttatc tcaaacaagt attggcggat
     3601 atcaggttct ttagcaaagt ccgcaattcg ttccaattca ctgatgactt gattcagctc
     3661 cattcgagca tccgtttcct catcaattac atacggatca tcagggactt tcaccccgtt
     3721 tttgcgaata tcaaaattga gccggtacag ttcttcttcc aattccggat ctgactgaat
     3781 cttgccaatc acccagtaga ttccccaatc aactcatcat aaatcatctg tttttacctc
     3841 ctttaatctt ctagcaatat caaaaatgac cggaactgtt acagaatttc cagcctgctt
     3901 atacaactga ctatctgaat tgacttgaga agctcgatca aaagcccagt ccggaaagcc
     3961 ctgaagccgc cagcactccc taggtgttaa ctttcgaatt tgatactgat ccgtgacgac
     4021 tgcttgttgg tctcctgtta ataaggtatg cacactttgt tttcccagac ggcctcgacg
     4081 acttgtggaa ttaggaaaag ccaggttcac actgttccct ggcttcacct cttgatattc
     4141 tgtttcgttt ttccgaagca tgatcttcgt tccgccttgt ttcgttgaca aagtaggcgc
     4201 tgttccagtt atctcataaa cccgattgcc ttgttcaaaa tttcccagta acatacccgc
     4261 tacagaaatt ccatcatcta ccggcaatgc gattttgatt ccttcatcac cggtcaaggt
     4321 aggcgctacg tcatctgacc catagacttg tccccccatt cctcgattgg aagggttgat
     4381 atttaataag tttctgatgt tttgatcaga tgttggagac gatccaatga taggaaatac
     4441 ttgttcggta catgcgtctc gagaatgtcc gataaggaag atccgctcac ggttttgggg
     4501 aacgtaggcc tttgagttaa gcacttgcca ttccacatca taccccaatc catccagcgt
     4561 tcggaggatc gtctcgaacg tagcccctcc ttcgtgattg agtaatcccc ggacgttctc
     4621 aaggaataaa aacttaggtt ggagaatagc ggcgaaccga gcgatttcaa agaataaagt
     4681 acctcgagta tcggaaaatc cttgccgctt tcccgcaatt gaaaaagctt ggcacggaaa
     4741 tccaccgcaa aggatatcca ctggtccgag agattgaata aactcgtctg atacacttgt
     4801 gatgtcatgc atttccacct ccttacttgt atcatggatt gccttataac tctggcgagc
     4861 aaaatcatca atttcacaaa agccgataca ctggtgaccc gcttgttcca tacctaagca
     4921 aaagccacca atcccagcga ataaatctaa aaatgtcata aatcacctcg taaaaaaagc
     4981 ccaggattct tgacactcct aggctacata ctattatttt atccttccgt attaacgttt
     5041 aatccaccaa tgtcgctaat ttcccttaag aaatcatatc cttttggtac aagtggcgta
     5101 taaaactcac taatcacact ccctcccgta tctacatagc ctcgaccttt gatacgcttc
     5161 ataaagaatt ttttatcaac ctcgccaaac atcatggaat atcccagttc actcatacga
     5221 cccaaagcca cccagaaatt aaattgatcg cgaatcccgt ctcctaaata tttcgcatcc
     5281 ggtctttgac aagctagaat cagaaagaaa ccagattgac gacctaacat cacgatttgt
     5341 ttgagcttat tcaaaatcac cgcactttcc ttcgtcgtta acatttccat ataaaccacg
     5401 tattcatcaa agattaaaaa gtttggtgga agtccaagaa acgcataatt ctctcctgtt
     5461 ttgtagttgg gcatttcttt cattgccttg ctacgagtca tcatgcgctc atagaaatct
     5521 tccacacaag cagaaatttc ctccttttgg gaatagacat gaggcattac cgtacctaaa
     5581 tcggctaagt ccgcattttt gggatcaaga acaaaaagct cggcatcaga ttttaataaa
     5641 gcctcgatga tagtgagtaa aaagtaagtc ttcccaccac ctgtgccgcc agcgattaac
     5701 atatgaggta aagaatcata agcccatact tgatttttca tcaatcgcaa agccccactt
     5761 tctgccacta cttcgtctat cccaattcga ttggcaatta tatcatacag taaagtgtac
     5821 tccacatagg agtctttgag ttccttttct actagttcac aatatagacc actttctaac
     5881 ttcttttcca gctttaaaag ctgatcttga tattttccca gtgaaatttg aacgcgaata
     5941 gaaagtaaac catccttcat acggtaatag attttgggga agtaactgat tgtctctttg
     6001 gttcgactac tattgaggtc tttgaaaaat ccttcactct gagtttgttt tacttcgtac
     6061 caatgatttt ctagaatcat ccgggctagc ttttgtcgat gccacaaact gcgccaatga
     6121 tcctgaaaaa agtgtatata aagcaacatc gtgcttcctg aaagaacgag acttcctagc
     6181 aaatagatta aatcttttat agaacgaacg atttctatag tagaaagtga tagcttagaa
     6241 atatcaaatt gtctaagctc ttgaaaataa aagacgctca tccaaccgat gaacagagcg
     6301 agtagccagc caagaacaaa gtgataaacc aagtgctgat ctcctgcccg tatacgatgc
     6361 cctttgtaca tttttatggt ttccacctcc aatcgaaaaa agcttcatag gatttcattg
     6421 atccaatttc aagcagttat gctagtcatt tactgctata gggagttgtt tcgtttccac
     6481 tagttgcttt aaaaaaactt caatggcatc cggcatttcc acaccaattt cttccaatac
     6541 ggtttcggct tctcgacata gactttcatt tacttcgata accataaaga aggactcttc
     6601 ttcccctaac caattcagta catcaaaatc ttcttcggca tcgttaccta cttccagtaa
     6661 cccttgatgc ataatcagaa acattttttc atcataggtt aatgctttag tgagttgttc
     6721 ctccgttagc tcaccttttc catattgatc gagacaatct aaaatatgtt caacattaat
     6781 ttccatacgc ttcacctatt tctttccttc attttggttt gtagaatttc cctgatctgg
     6841 ttgtcgtttc aagactaaat catcggcctt aatgaaccaa ttgacagtag ttccttctcg
     6901 gaaaacataa ttggcgaccg tatccatgat tggattgacc aactctacct ctgcgtcata
     6961 ggcaaactcg cgtaagggta catttgctgg gatactaact tggatcattc gaccttgcgc
     7021 agaggatttc aaattataag tccgttcttt tacctcgtta ctaaccgttc catcctcatt
     7081 ttgcttccgt acttctcggc gtaatgcgga aaatcgtaat ttcccaaacg ttagtcctga
     7141 atctacaaca ataccttctg ttaatctcat cttcttttcc tcctatgctt tcactaaatc
     7201 atcgacatac aacacgtagt cggtaaaccc acgattctca atctcatagc ctttggcaac
     7261 caaatgggga ttcacggctt ttagtggttg atcttgatca aaatcctttt cgccggcttc
     7321 cgcagctacc accacttcaa tatcatcggc tcgttgctta ctggaataca aatgataact
     7381 acgaccaatt actgtagtat tccgcccata tccttcagtt aaaacttcgc cttcgccggc
     7441 ataccttatc tttccaaagg tttcggccat atctggcaca acaaatttca attccatctt
     7501 cttttcctct tttcattttt agttttggat acaaaaatag cgcgaccact ttttttagga
     7561 ggcggtcgcg cacgttggtt tttcctattt agttgtttca tcacatcacc ttagtttcgg
     7621 cgattccaga aataataccc agcagtcgca aagatcaagg taaacccaat aaacagcaga
     7681 acattagaat ttttctcccc tgtttgtggg aatgtcttta ctgtaggact agattcttga
     7741 gagtcacttg gaacagttgg tatttccggt tgttctggtg cacttggtgt tgtcggaact
     7801 tttttaggtg ttaaagtttg agttttttct tttagatcct cattgtgttt cccattgatg
     7861 tttccgtctt tgccatagtt aatttctttg aaggtaaaac ttgtaccttc tggatattta
     7921 cttgtgtcca ctttttccgc aagtacggtt ttagtgaatt ctttgtcatt cacttcataa
     7981 tcaattttgc cagatttcca aatttctttg gtcgtattat ccggaagtaa tgcatacaga
     8041 attgtttcaa aagcttcttt tgagccatcc agtacatcat gggtaatcga cacatcatcg
     8101 aacatatcca ccacatcccc atgagtaaag gtttgcgaac catcttctag gtgggctttt
     8161 gtttggatgg aaacatggcg ttccaccgta caattgacag tttgggcttg attgttcaag
     8221 ctagcatctt tcgctactgg atcatcacct gcttcatagg gtttcttgtt ttcatacaca
     8281 tagttgaata agacgatggt tttatccaaa acttgttctg cggttaattt atgcaaaatt
     8341 ttccaagttc ctgttttctc tttattcgcc aacgttgcgg ttgtttcggc aattaccact
     8401 ggtttggctt tttcatcttt ttcttgggca gcttttgtcg cttccacatc aatggcttgc
     8461 gctaccacat accagtcttc tttgatttca tgcaagttat agctcaattt atcaactagc
     8521 tcggtagaat caaggtctgt caattctttg cctccgtctt tccaagtccc aagagaagtt
     8581 aagctatctt cttcttcagg taaatcatag agcatcaaac gattcaagct aacagaaaat
     8641 gctttattcg tcagtttctc gtaaggaacg gtcaccgttt taattggttg tttttgtcct
     8701 ttttcggtaa tcgtaaaaat gtattcactc ttagtgtaat ccgctttatt ttctttaaaa
     8761 gtagaacgga tttctaatgg cgtaattttt tgaaatcctt ctggagcctc tacttcttca
     8821 agtaaataat ccccataagg aagattttca aacttgccat agccatcaaa acctagttgc
     8881 tcgttataag ctgtaattgc ttcatcttcg gcatctgtaa tctcactggt tccttccaat
     8941 ggcgatactt taaaggtcag atcattgaag ccagtttcgg cagtcccagc agctgatcca
     9001 gcaaatttaa agaaatcaaa gccaaagcga atgacttgtt cttttgccgt aacatctcgg
     9061 gtaatcacgg catttttttc gttatcatcc acctttttga tggaaacagg gtactttgtt
     9121 tcatccaagg tatatccttc aggtgctttg gtttcttgcc agtaatactc attaatcgct
     9181 aagtgtttaa cagcaacttg attcttttca tctaaagtca aagtcaccgt ttcatcagaa
     9241 gctttcgttc ccttcactaa ctctggttta aaagcctcac tccatttgac cgcttgacca
     9301 tcttttgcag taaagagagt atattcagct cctttaaact cagctttacc ttgagtttca
     9361 ttaccggtat ctttgtcttc ttttgtcaaa gtggtttcac cagtgatttc ttggttttgc
     9421 ccttttacgt tactggtaac aagagccacg gtttgattga catagttcaa ttcgactttt
     9481 actggtttaa aggtattcac aaagccatta ctagctttgg tttcggtcac ataataggtt
     9541 cccaattcca acgcattggc aatctctttt ggtgtttccg catgaccttt ttcatccgtg
     9601 gtcatttctt ggacaatttc accggttgga ctgtctttgc gaatggcaaa cgtatttccg
     9661 gctaatgagt atttgtcgtt ccaaagatca gttcctgttt ccacccccat tttattcaaa
     9721 atgatttgac ctttttcgcg agtatttttt gaagtcacag aaatagtctc accagattta
     9781 atggttgcgg tgattggtgt agtgtcaatc gtataaggag ctggcactga tttttcagta
     9841 atggttactt tcgcgccatg aggaatctca tccaatgtcg caatgccttc tttatcggtg
     9901 gtcacgtctt ttgcaggtaa tgtttttcca aagtctaaat ggaaaacggt tcccggtaca
     9961 acatttcctg tttctttatc gactttttta atcttcaaag aaccctcagt ttccacatca
    10021 attttgattg tgtaggtatt gggcttatca attgcccccg ccatcaaagt ttgttgacca
    10081 gccattttat aagctaccgg tgttcctgta ttagctgact tctttagggt taattcacca
    10141 ctctcttttg aattggcatt tggagtaatc actagttgat tgccgttcac acgataatca
    10201 atatttgcga tattctcgac aacttcatca aactccgata aattcaagcc gttcgtatct
    10261 gtcacagtag tcgattgacc taacacggtt ttcactgtgg agttatggaa gcttggtttc
    10321 ttttgataat ccgcaatggc ttgattgatt tttgactcaa tggctgcgat atttactgca
    10381 ctaccatcag aacgtttaat tgagtgaagc gtgtagccat taacttcttg ccaaatcatc
    10441 ttctgggcca caatatgtgt atccacatcc gtaccagctt ccttccataa aacggaaact
    10501 aatttcgctt tctccgacat atcaggcaat ggatttttct cataaccagg agtgaactca
    10561 ttgtaaatcg gcacccctgg ctcaatacag aaaatatctt gcttcacacc attgtaaact
    10621 gcatagtgcg gaacggtaac ttctgacatg ggacgaccat cgctaaatgt accttcgaca
    10681 gcatactggt gagccaaatc caattccaca gttaccgttt gtggatgagt aatttcatca
    10741 gcaaaggcaa tcgttgggcc aagcgatcca ccaattgttt ggcataatag actcagtgtt
    10801 gcgagcaaac gccaattctt aaatcttggc tttttcataa aataaatctc ctttcgtttt
    10861 tgggtaataa aaaagacgtc caaattttaa ttttgaacgt ctgatttaga gtgatactat
    10921 ttagttttct ttgtcttgga taggttgttt ctctgcttat aaattttgat tcttcgataa
    10981 tcaaatccaa tctctgccat caattcctcc caatttttgt tcgttctttt cataattgta
    11041 tcaactgatg gtagttttgg ggattgaact agttttcgaa actttgtcat tgataaatca
    11101 tcctgtagtt ttaatcggat tatttcattt tttagttcta acagcagttc gaaatcagta
    11161 aatcgagatc catcattttt ttctttaaaa agtggacgta tatctcctaa catcttttta
    11221 atggtactaa gtgaaggaat attcttccct actgattttt gttcgtacat tcgttgagag
    11281 gttatcttct cgcacttaat aaaggcttct actatttcca ataattcttg ttctgacatt
    11341 ctagcccatt ttccataccc agtattttct cgtcctatac taatcaataa attctcccat
    11401 gatccatatt ttttgttaat gaaccacata cttggtgcag aatttggatt ttctgatagc
    11461 tttttctggt attgacggct agatgtaatt ccgtgttttt cgatgaattc attaattgtt
    11521 aaaatcagtt ttaactcact ttgatttttc caaatgatac gtccatcatt ttgcttcacg
    11581 ataaaaactc ctaatagtta tttagataga agcctatcac atcaactttt tagtagaaaa
    11641 gtacatgttt tgtctttttt gctaatagct gaccctttga gaaacttggg ttcaaaagtt
    11701 gctaaattcg gtaaatgttt tggtattttt cgtattatat ttgtcgtcaa gctgactact
    11761 aaattttgcc ctattttgac gacactgata gtcaatgtcg gacaatctag gacaattttt
    11821 aagggaatat gattgaaaaa tggtccaaaa cagccaaaaa tggcttaaaa ctgtgataga
    11881 gaaaaaagaa aaagcccttg atatacaagg acttttcaca aatttcagat ttccaaggaa
    11941 gttttccgtg tttatcttct gatttctttg atacgagcag cttttccgtg taatgcacgt
    12001 aaacaaaacc actatattct attatgtatc taataccctc aaaacagcta taaatcaaca
    12061 ataccgacat ataataaaca ctatgttcaa tctgagatat atcaaaggta aacgtatttt
    12121 tgcccccttt ttgcccctca aatatcgctt tacaacgaga acgtaagttc gtataatcgt
    12181 tttgaggtga tttttatgat ggaagacttt attagaaaaa atatcagtga tgaatatgca
    12241 gacttttatg agcaaagcag caaaaaagat aaattccaga tggatgtttc aatcttagct
    12301 atattagctt tttccgaaaa taaccaacct gtaattgcga aaaaagaaac agtattatct
    12361 gaaggcaaaa taaaaactcg atatatatta gaggtagaaa ctaagtttaa aaatagatcg
    12421 gagtaatggc tatgcttttt aatgagacac aattatggtt taaatttgat ccttcgaata
    12481 gatttatcaa agatttttat aaggtgtggg attcagaagt tttctttcta gcaatcgaag
    12541 atagcttatt aatcaatctc tactattcta ataagaacta ctttaaaatc cctgctgcaa
    12601 aaactagaat gaagaaagat gtatactttt tgtttgatat cgtgactgac gtgccggacg
    12661 cacgtagcga tcatcggcgt tatgactata taaagtatac tttcgttgat ccagaaaggt
    12721 ataaagatta aagtaggcta cctaaaaagg tagcccggaa cgaattttat caccatactt
    12781 gtgaaaggag atatttttta agttagtatt aagattgtgt aatatgatga tatctatatt
    12841 ttatagtatc agtgctataa aatcaaaaat aagtcactaa ttaactacca ctccaattat
    12901 aagccttttt tctcactttt tttcaaaaat atggtatgct ttttaatggc ttcaaatata
    12961 aaagagttta aagcgtaaca cacttatggg gaagtggttt gaggtgcgct ttaaactctt
    13021 ctttattatt atctcacaat ttaacccaaa tgtctttcta tttaaaaatc aaagtaaaac
    13081 ttttcaaata tacagaaata taactatgtg aaacatcctt tcattaatcc ataaaaggat
    13141 acataaaaaa gccactcatt tgagtggcaa tgaagaaaag ctttagcttg tataatactc
    13201 ttcaaaaaat tctaacacag aacgattcaa atggctacgt taatgtaccc tgtaggactc
    13261 gaacctacga ccggacggtt atgagccgtc tgctctgacc aactgagcta agggtactgg
    13321 ttgttgccac ataaagccat aaacaatcaa ccagtagaat gtgtggcaac aaacctgtta
    13381 tcgcatagct tggagtgtga ctattatggg tgatagtgaa gatatgcgat aacatcacta
    13441 ttttatcgaa tgatttttat agttgtcaat atagttatgt aatgctcctc aacgaggagc
    13501 tatttttatc gtttaggaat atttaaatac caacgtttgt catggaaatc ttgcgcaccg
    13561 cctttagtgt ttccctctgg atcattcgtt gcccgcatca tgacatagac tttcttatta
    13621 gggaagttac gcatgttaaa agatacatga tagccaacat ttccagaagt attataagct
    13681 tgatttacat ctgatctata aattccatca gctcttactc gagctaattc tttcccagta
    13741 ttgtaatcca taatgaagat atactcgtat ttatagttag caatgtgcca tccagctaca
    13801 tgcaaatttg cattttcgat ttctccaaac tgatcaatgt gggcgtaatt cgttccatct
    13861 gtcagtgtag gattagctgc accagctcgt gttggatcaa tgacaggctt gttttcagaa
    13921 gttgttggat tttcatcggt aaatccatga gctaaatcat atgctaattt ttctttactt
    13981 acgcccattt cagaaagata accgtaagga tctgtatgat cgccccagat gttttgtgtt
    14041 acccataaat gcgatttgat tcccggttgg ttataaggag tgtccaacgt taatggaata
    14101 ccatatttca ttgctgaatc tctagccaat tcaacgtatg ccttgtagtt tttctcaaac
    14161 gttgctttat catgtgtgtg ttgtaactca atctgcacag gactgttggc attagcatac
    14221 gaaccagcac cgtactgtac ataaccaggt tgaccgactt gataaacaat tccgccgtct
    14281 cccacaatat aagcagtata agcgctagtc catgaacgtt gcatatactg cgcttcattg
    14341 cgtcctgttg ctgtttcatt agccgtttca tgcagtaaaa tatactgatt atttgctact
    14401 tgtgagctac cttcatttgc gcccaaatta aattcattgt tgatagtata ggcaaatccg
    14461 ttaattggca ataaaaaaag agccgttaac aggctcatcg cagtaatagt aattttcttt
    14521 ttcatttgtt tcctccttct tcgctttcag ccgagaacat tttgtaggtt cgatttgata
    14581 cacccaacac actccctaaa aacgcgccaa aaccagtaat gatgacaaca cagatatctg
    14641 tgtactgcca attgagcgct ttaccaacta accccacgaa agtagctagt gcgggaataa
    14701 ttaccagtgc gaaccatttt agtacttcga acgttttatt attcattttc ttctctccct
    14761 aaacaaagtt ttgatttgtt gcgtgtgttc caccaatttt tctgtatgtg tatctaatct
    14821 ttcatcgtgt ttctttagtt cttcatgaat catcaatcga tctgatttgc tcgattctaa
    14881 atctttagtc agcaaatcta aattgtggct tacttttgaa agagtctcag taattttcga
    14941 gaaagatgca gcaatcggtc taattactaa taaaatcaaa gaaacgatag cggttattga
    15001 tcctgctatc attccccatt cccctaaatt aatcatgtga caactccttt accttaaata
    15061 aaaacgcatc aattaagatg cgctcttatc tttattaatg attttatctg cttcttcgtc
    15121 tgtaatgcat aatggaacga aaaccattac ttgttcgtta gtgaaacagc cccagtcata
    15181 cattagtttg atatcttcat atgaatacat gttacttccc accttgtaat tgttttttga
    15241 tttcttcgat ttccttggta ttttgtacag aagcaagcat cgtttttgaa ttcaactgcg
    15301 ctaaactttc tgacttttct ttcaataaga cattttcttg cttgattgct acattgttta
    15361 gcattgcttt agaatttagc tgtttcaatt catcattttc tgccttcagc gtttcgtata
    15421 atgccttaat attagccaat tcatctacgc tatcaacagc attccctttt ttctcttctt
    15481 cagtagctaa ctcaacccac tgttttttat caaaatcaaa tcttggtctc cagttaggaa
    15541 ctggtggttt aatctctgta cagttttcag ggatattttc ttgattgttc aaaatgattt
    15601 gctcgaatcc gtaaggcttg attgatttgt aaactacttt cattagattt cctcccttaa
    15661 attgaataag tgataacaaa tgaataagct gatccataac ttgaatttct tctccattta
    15721 atggctccgt ctgcgccaat agatagttga gcactgttca aagtagaacg gtctattgac
    15781 ccaaccagtt gttcaaaact aattggtgtc cgatagcctt ctggaacagt taaaataacc
    15841 gaatcattcc cgccactact ttttccgttt aaagccacaa aatatataga aactgttttt
    15901 ccttcacgat aaagctttgc tgtccctgta ttaccgttag tgacaaccag atttaccact
    15961 tcatatctgt tatcatcagt cgtcaaaaca cgtttattat tgattgttac agaacctgaa
    16021 aaattcttat gtcctgcaat ggcttggtct ccatctgtct gcaccaatgc ttctttacca
    16081 tcaatagctt ctacatgcgt ttttaagtat ttagcaactc cgtcttcttt taattgaaca
    16141 atatcagcca ttaaaccgtc cccactttct caaatgtaat attcgctaat ccatcaagtt
    16201 tcactttatc ggttgctgac attaaacctg ctgtcgtagt tgtagcatta cctggatttt
    16261 tctgtgctcc agctgcaatt ccatccaact tagttttatc tgtggacgac atcaacccat
    16321 ttgctgtagt tgtagctaca gctgtagttg tggcatttat tccaggatca cctttatctc
    16381 cctttggtaa aacaaaatta aatctagctg cagatgatgt tcctacattc gtaacagaag
    16441 cggttgaacc actagaaacg gttcctatgg taattgttgc tgcttggcca ggatcgcctt
    16501 tatctccctt caccgttgtt ggtttgcctt ctatagcatt ccaatgagtt tgtggataaa
    16561 cctgtacacc gctttgtttt atttttacga tatctgtcat tttctatacc tccccgattt
    16621 tttcaaaagt aaaatttgga attctttcgt ttgtgtaatt ttctgcttga tttacagctt
    16681 cttggaattt ttgatctaca tatgactgat taacaccacc agtcccacta ccacctgtag
    16741 aactaattgt tccatcttct gcaattgata tatttgcacc tgcttttaat atttttagag
    16801 attctaattt tccctttaat tcttcagaga aattgaagtc tgtttgctta gttgcagata
    16861 aaacaccttc ttcagtaact tctaagagtt ccccaacttt tatacctcct aattgttcag
    16921 gagtagcaat cggcaaaata tatgttccac cttctccatt tacgatcttt tgaaacattt
    16981 cagcagtaag aataccatcg cttgtctcgc ttgcataagg tagttcagta attgcattct
    17041 ctaatcctaa atcagctttc gttaaaatga ctgcaccata tttaccatta accgaaagaa
    17101 cttttgattg tcctgatatc attttttcta agcctaaaac tgcagaaaca tgtgtaattg
    17161 gcataaattg acgtttaaca ccagattcat cagtctccat cattctcttt actttaacca
    17221 tcaaatcacc ccaactttct caagagtgaa aacattctgt tttggatcat caacagttgc
    17281 aataatcaaa gctccttctt caattggaaa ttctactgtt tcaatttttt cgacttcgtg
    17341 attatctgaa aagagatcgt cttgaagaat atcagtcact tcaatttcac cgtattcaat
    17401 cgtgaaaagt gctgctttca ttttttgata caaataattt agatcggata ggaaacgttc
    17461 agaaatcgat tcatggcgta caccttgaag atctacacgt gcatccatta attcggctaa
    17521 cattactcca cctggatcga tagacttcaa aatatccttg attgattcaa accatgacaa
    17581 ataatctgat tcttgtcctt ctctccaatc ttggaaacta ttctcctgtt cttctctcca
    17641 gcgatcaaat tcttctttcc tttcattcat ccagtccgta aaatcgcctt tattttcatt
    17701 aataaaatct gtcatatcag caattaaatc ttcaattgat tgccaataag aacccatttc
    17761 tccttctgtt tttgaaacag cattgatgac aaaataggag aagtcttgag ttgttccaat
    17821 caagtcttct cctttaaata taatgaaatt ggctgtttgt cgatgcaaac actgcatgga
    17881 atatttatca aaaatatatt tgattttccc ttttttagca tccacaattc ttgtttctaa
    17941 ttggactgga tattttccgc caacaactga ttcaaaatat accttacatt ctgataaatc
    18001 atatggaaga ccattttcga caattgtagc ttccataact tcagtgtttt tattgccttg
    18061 tcgaacttga atcatcccca cgtaattata gggttctgtt gtacttaata tgacattcca
    18121 ctttgccatt aaatcacctc cctattttgg tggtatgaca atggaagaaa ttgcacttgc
    18181 actataatac tggcgatcta actttccaca aatcatacct aactctgtgt tttgttcata
    18241 agtttgcata cgaccattag ctaagcctcg gataacgcca gtatgtccat aagttccgtc
    18301 tgcaaaccat gaacccactt gtccacctct tgcccaatta ataattgcac caactactaa
    18361 ttgatcatat ctagggtttt gaatcacttt ccaaccaaca gcagaccaat cataggcaat
    18421 accaatatca gatgctgcag aagtatttcc tatcacatga gttaaaccat attttgttcc
    18481 agcacccatg ccacaaccac ccagatatcc tgaatattct gcagacaagc cataacactg
    18541 accattgcca attcgttgtc caatcaaaga ctctaaatgt tttagtcctg cttctccagt
    18601 agctcctcct ggttttaaat ctttaaattt gttataccag ttaactgcat aatcttgacg
    18661 ttcaggatgt gtcgctgccg gacgttcata gtttcgttca aaagcatatg ctgcttgtct
    18721 tggatcagta caggccttaa atccatcaac cgtcgtaggt tgtactacgc ccatccattg
    18781 tccatttgta aatgtccaaa taagcaatcg aacttgtgca tctaagctcg ttattggttc
    18841 tttaatacct gcagcattaa ataagttttg aacataaact tttccatccc atgttgctgg
    18901 accaacaaga ggatatgaag aaccatccca ctgaactaat ccgtatgctg gtccacctat
    18961 ttgaacagta tcaggatcaa aagttcctcc tgtttcttgt tgaatgttcc ccaatattcc
    19021 acatgcagat tgtttcgtaa atccgttatt acacaaaata tcgtatattc tccaagctct
    19081 cttttctgca tctgttttta attcatcagg atacccacca ggtgtttctc ctccgccaga
    19141 tggaccgcca ttttgtcctg gtatcacttc tttacctcta acagtaagtt ttccctgaat
    19201 atctaaatca ccgaaataat atgcttttcc gtttcctaat aacacaaatc ctttttctgc
    19261 agttggagaa attaaaatgt attttccatc gccatttgta cgtattacca aagaattttc
    19321 ctcaagaggt gtaggtgtta cagcatttgg aaatggattt cctgctgaat ctgttgttcc
    19381 gatcgttcca atggattctt ttctattcca gaattccata ccttttttgg ttagctccat
    19441 gatttttttg ttttcattcc aaatttgaag cgtgcctttt acaagtttta acacatcacc
    19501 ataagcatta aaggaagttt cgaatacttc tgcatttatt gttcctactt tgatgaagtc
    19561 agcaacaatt tctccttttg atgtgatagc aataccgaac gggccattta ctccgttgtc
    19621 tgaataacct aagccattta aattccaacg ccacactcgc tttgcattcg ccacatttgg
    19681 agtatccatg ataagaatct ctgatggagc tttttctgga cgaaaaacga catgcccacc
    19741 agaattacca gtgatccatg ccgtcgcatt caacacattt tgaactaacg tttctgttcg
    19801 attgtcgatt ttctttttca attcttgtgc ttgagtattt actgccgaag tgtacagtga
    19861 taaatcatta cctaaaacaa tatccttata tttcccaagc gttgggaaat aagtgtattc
    19921 gaccatacgc tcttttatct cgatatctag ctctttcgcc ctaacatgag cgacatcacc
    19981 aaagtgtaaa gacgctagct cttgatacat gtcaccgtat tcgagtgtgt gttccaacgc
    20041 caccatactg actgtatgag tagcttttgg ctcatggata cgatcattgt caaataaggt
    20101 tttgccccat ttgatcaatt catcaactgt tttacaatct ccgttttcac gtttggcaat
    20161 acgtcgattc tcgttggtta ctccatcaat ttctaaataa ccatattcga taggttcttt
    20221 gtcttcatca taatcgttat caggaacacc gccaaccaaa tacaaactat tgacaattga
    20281 ttcttcatca atttcttctt cgattgcttc taaattgatg ccaaaatcaa tccgaaaacc
    20341 attatctgat ccaatctgtt ttaccagttt caaatcatag ttatccatat caagttccgc
    20401 gctagtcacg ccagtcaaat tttgattacc gttatttgaa ccaatgattg cttcaactgg
    20461 cgcgacttgt cttgctgtaa attggtgcgt tgtccctaca ttcgaaagat agttaaattt
    20521 ttgatcaaat gccaaactgt ttttaagatt ggtcatgatc tgagacccat tgccgttatc
    20581 cgtaaacgac ttgacaataa agttcttatt ggccataaaa ccaatatgcc gagctgttac
    20641 agaaacggag gtcaaattct ttttgacatt ataaatttca aaatattgcc atgatccatc
    20701 tggtaccttc gcttttataa aatttccttt ttttaaataa gagcgatact gtccgcttct
    20761 tgaatagtta ccataaaaac gatattggcc attcagcaca cgattgattt ctggcaaatc
    20821 ttcccaatca actaacgacg ctccgttcac gctcaaatca tctggcattt ttttataagc
    20881 ataaataaat tcttgcgtca cagatacaca ctcctattcc aaaaccgcac ttctttaaat
    20941 tttccggata tttttacctg attccattca ggctgcaata ctggccaatc accacgcgta
    21001 aataaattca aaccttcttg tattgctttt ccaagctgag tatcaaccac gattgttgca
    21061 gctaacgtat taagtattgt taggctttta tcaccgacag cgatcgttat atctcctccg
    21121 ttcgattcta tttccagata gggatgtgca atttcgtcac catgatcaaa aatatccata
    21181 acactagaat gaaaaatttt aggtgcttca ccgatttttc ttttgagtgg ctgacaacga
    21241 aaagtaacat cgaatgtata aaagaagccc cactcgtttt cgaaagggac ttctttatcc
    21301 atgctgcaga tagcctctag atacttgtct ggatcattat gtgtgattaa ttggctttta
    21361 ccagtgagcc atcgtttgac ttctctcagt ttcgaatgtg gaatagtgat cccttctatt
    21421 tccaaatcaa aaggttcata gtcatcaaac gtctccgtca attctccgct tcggccctga
    21481 atcgtgtaag tttcgtatcg cttgttcggc ataatatctg gaagctctgc ctcaatgatg
    21541 caattcatat caattactgc attccgattt ttccaaataa aatttggttc atctggattc
    21601 ataaattgtc gactcaaata ggaacacctc ccaaatcacg aatagcctgt ttgtttgctt
    21661 tagcaaattt acgattcata cgatctaact cagatggatt gtttgcatcg actttgccaa
    21721 tgtgtatatg ctgttcaatg ctacctccag aaactcttcc actgatccct ttgcgttttt
    21781 cttcgtctga taatggagta actgtggttt taccattctt ggcagtcagt aactcaggtc
    21841 cagcttcacc aacaatcgct tgtccattga ttagatgacc gccttgtgct aaataaggca
    21901 gttttgaaat agaaaatgat tttccaccta caccaggaac ccattttggt atctttatat
    21961 tattcaatcc accaataaat ccattgatca atccaatcat ggcattaata ggagctttcc
    22021 ctacagcaac aataccatcg aaaatacctc caaaaatatc aactagacct tgccatgctt
    22081 ttgaccaatt ccctgtaaat actccagtga taaaatctag aaaaccactg aaaattcgtt
    22141 ttccagcatt ataaaaatta ttgaaatttg ctataacacc atcgaaaaat ccaccaaaat
    22201 aaccacttaa aaaattaaat atttcaacag ctacatctga aacacctttg aagaaagaat
    22261 tcactccatc atgaaaccat ttaacgttat tatatgccca aattagtcca gtaactaatg
    22321 cgccaatagc tataactgct aatgcaaacc agcctcccga cattccaaat aaaccagcaa
    22381 gtccttgcca aactcctata aaacttttaa cttccccaac gatttttgta acagatccca
    22441 taagtgaacc aagaacgatt agtactggtc caactgctgc cgctatacca gcaattgtta
    22501 tgatccaatt ctttgtatct tgatctagag acccccacca tttagagaat tcctgtaata
    22561 aggaagttgc tttttcaaaa aaaggcaaaa ggcttacttg gactgcttca cctacgtcag
    22621 ccattgctaa ttttgcattg ttcatagctt gatcggcttg atcaattggg tcgagagtag
    22681 catcgaatgt gtctcctaca gctcccccac tttctccagc agtttttgct aaatcttcca
    22741 ggttcaaagt acctctacga attgcatcgg ccattctagg accgccttta gtcccgaata
    22801 cttctgctgc tgcattgatg gcttctgttt cagaacctgc attttttacc ttgtcttgta
    22861 gttcttctag tccttgactt aaagattttc cgtctttcgc ataagccact gttgctttag
    22921 ataaactact taaagctgcg cttgaatcaa caccagattg ctcaaattgt ccaagtaaag
    22981 tgaccccttc accaaagctc aaccctaatt gtttaatttg tggtgcgcca tcaattgctt
    23041 tttgcatcaa gtcgtctaca gattgaccag tattctgcga tgttttcgtt gttacatcaa
    23101 ggacagaatt caaatcatca tattctagtc cataagcatc aatagcttgt cgagcagata
    23161 ttgctgattg cgaaacatct gtatcattga tttcagcgta ctgtaggaga taattggttg
    23221 aatcttccag ttttttatcc atgaatccaa attgtgtatt tacctcacca atagcttccc
    23281 caaccgtttg taaaggtaaa tgagtatttg aaccaacgtt tttgaaagac tgtgaaagtc
    23341 tatcagcttg atcacctgtt gctccggttt ttgtaatgat ggtatcaagt gcctcgtcaa
    23401 cttcaccaaa tgctgcaagt cctgctgctc ctgccgctac aataggcgcg gttactccaa
    23461 cagacatttt ttcaccaacg ccttttactt tctcacctgt ttcttcgatt ttctggagtt
    23521 tttttgctgt atcaacagaa acgtcacctt gttctttaag tgcatcgttc gtctgttcta
    23581 atgctgtacg aagtttattt tctccggttt cagaattgag taattgttta taaagttttt
    23641 ctgattcagc agaatattca ccggtttctt tgacagattt ttcatattcc tctctcagca
    23701 tttgagatcg ttgttcagcc aatcctaatt gtttttctaa tttttgttta gccgctcgca
    23761 atttttctgt ttgagttgca tccttgtcca tcgcagacac ttggtttttg tactcggcag
    23821 cggctaagtt catttctttg ttgatttctt tgattgtttt cgaataactg acttcgccat
    23881 tcgttttaaa attaagaacg acatcagatt ctttaccagc catttatctt agcgctcctt
    23941 tctaccacca aggcgattta tccatagtta cagattgagg tggttcaaaa tcggtgtttt
    24001 gttgtaacca ctgtaaataa gatttgagcc acaagttagg tgtagacttt aaaaagaaac
    24061 tctcactcca attcaataga gtgagagcaa cgtaaatata aaaactccaa ggagttccta
    24121 tctcttccga ttctttttgt ttttctttct ttttgcttgc gctttttgaa agtcttgtgg
    24181 cttcttggat ttttttaggt cttccacctg aaatgtctgg ctagcaaaaa tttccataca
    24241 ggtactataa gcagacaaca cctctccact cattcccaaa aatttgaaaa tagtttctgg
    24301 atcttcctct aaaccaccag tacgcagcat ggcataaatc aaggcacgca tgatttttag
    24361 atcactaggc gataagttag cagaagaaat ttgtccttct tttttagata gcatggcgtt
    24421 catatcagat tcaaattgcg aataatctcc accatacaca tctgctataa attccatcgt
    24481 ttgcatagta aatgaaatag ggaattctgc gccttgaata attacaacag cagaattttt
    24541 taagtcttca acgtgaattc cataatcaga taaccgagcc attaacccgc acctccaact
    24601 ttagccaatg ttttccactg ttcttcatcg tatacaggtt gagcaatgaa tttattaaat
    24661 aattctagag aagctccgtc tctatttgaa tcaaaacttg catacatcac attattgtat
    24721 ttcagtccga tggaaaccag atttgcagtt acatcgtcaa tttttgtttc ttcctctgca
    24781 gtggcatact cttcgtcaat cacattggat agttgtgtat taggatacca aactgctttt
    24841 ttaccgccat tttcaatatt tccaataaat ccaaaggcga agtacggaaa ttctcgtgcg
    24901 gtatttttgc taaacgttac tccgctttta gcaactaatc ctttgatttc atccatcact
    24961 tctatcggaa tacccacgtg atccaacgca agttcatgtt ttgtttctcg cgacacacga
    25021 cgaaacattt tgctggatgc ccatttttct aatgctgttc cattcccttt gacaccgatt
    25081 tttgtggcaa ttggcaatcg taaaacttca ctataagtcg gtgcagttcc aacagaatct
    25141 ggcgttgcca tcatcgcaat taaaatgtcg tccaatcctt caaaataaaa agtatcttgt
    25201 tttcccaatt aaaatcaccc ttcccataaa tcaagtattt ttctagtcat aatttcttca
    25261 attttttctt tgttttgttc atacgtacca cttgcaaaat gttgggcacg ttgatttgtt
    25321 gtgccatttt cggcaaagcg ccagtaaaag gccgtctctt caaattgaac tttcacttga
    25381 tcgttttcaa ctactacttt tatttgatcg cgcatatgtt tcttttttaa gagcgactta
    25441 ggaatattag gaagcaactg ctttagataa aagttagctg cttcttctaa agattccaac
    25501 gataattttt tcggatcaac ttttgaaagg tttcctaaat aatccgatat ttcagaaaaa
    25561 ccattcttat tacttggcat tttccacaca cctcacatat gtgtaatagt tggttacggt
    25621 atcgtcgttc tcatcacctt gtatacctgt aaaatctgaa tatggaatgc cagcattttg
    25681 cagcgcttgt tcaataacaa ctaaatcctg ttctgttcca agtgtaaaaa aagagacttg
    25741 gtaataaggt aattttttat agactttacc ggatgccatt tttttgctgt tactcacatt
    25801 tgagtacaca atgtatggat acatcgtccc taatctggct ttgtctctga acactgctaa
    25861 ctttgttgat ttcagcgctg ttttcaattc atcaaagcta atcgacataa gctaaactca
    25921 actccatttc tcttgcatcg ggattcgtat aaatgcgagt aatgttatac gttacagaat
    25981 caattttgag cgcacttaat ttctctgtga tggatttatc ccatctgact ttaattcgtc
    26041 tgacaacgtc tgtcttggct tgctgtgata aatatttttc ttgagaagtc acaccgagtt
    26101 cttcatagaa tattagacgc ttaaattcgt aaattgtagt tggacgatcg tttccgtctg
    26161 ttcctgtttt gatgtctagc aattcggctt tccatctgag attattggtc tgtctcttcg
    26221 gcattttgaa tcgctccttg cacgataaat ggcgtcatgg cattcatagc tttgtcgagt
    26281 tcatcctctg aaactctata ttcataggca atgccggcaa ccatcaaaat aagatattct
    26341 tgttggccac cagttgctgt tttgacataa tcttttgcca tatttaaata aaaagagagc
    26401 aaagaatcat ccatgccctc ttcaaaatga atatgtgatt tgaatttttc ctctaaagac
    26461 aattctttag tttgctcttc catcttaacc accaactggt tttgtaattt cgtagcgata
    26521 tactgccggt tcaaatggag aataaaccaa ttgaccatct agcaagttgt aaatttggaa
    26581 tccaatttga tttttaccag agaatttttc aacaagtttt tgaatttcca aggcaccaat
    26641 aacttcttga attttaaatg cagaaaaatc gccaaaatat aaaactggcg tgtctggttc
    26701 acccttttta tctgctgcat ctgtccaatc cacaggatag ccaactaatt ggtaaccaat
    26761 tccaccttct gcttgtgtga atggccgcaa caaaggaaat ccatcatctg ttttcatttt
    26821 ttcaatggca gtcaaagcag ctcgattaat aataaagcgt ccctttttca tcacttctgt
    26881 cactggtgta tttttaaatt cgattaatgc atcatataat ttttgcccag cacctgaagc
    26941 agttagatct aaaggttttt taaatgctac agcctttttg gctaatgcac caggattttc
    27001 atttccagcg tcatcaccat tgaacatata attgatttct ttacgcacat aagctttttt
    27061 caattcttcc acaacaatat cttcaactgg aacaccagac atttttaata attttttagt
    27121 tactgttgcc aaagcatcga attcggcagg attaagcaaa atttcatcaa actgaatagc
    27181 tgtttcagca atatcagttg aacgctcttt cttgtttaca ttcgcatctg ctttcttcac
    27241 aagaattgga tatttgacat ctcctgatgt tcgcaccact gttccgtatt tacgaagtaa
    27301 attttcttct tgagcataag taataacttc agatgcaatt acttctggga cagtaactga
    27361 accgttgcca gcttcaatcc ctaaagctcg agcttctgct tcagaaatat ttccagctac
    27421 aaaattagca aatgcttttc gtagttgttg atctttttgt ttcttggtca tttttgcacg
    27481 tgcctccaat ccgttcttaa ttgatccaag taatccatct cgttgctgtt gagtaatcat
    27541 tccagaacga ttttctgaac tatcttcact gtctgaatct tcttcttgat cgttgtcttc
    27601 accagaacgg ctttcgtctg aatccgtact attcgattga tcatctgtat tgtcgtctgt
    27661 ttcatcagtt ccagaatctg cgcctaattc gtctttaatt ccgttcaatt catcaataac
    27721 actgtcaatt tcttctttca cggcttctaa atctgcttcg cgtaattctc ctgattcaac
    27781 tttttcacgt aattcagtca atcgttgctc acgacgtgcc ttcatttttt tcaataattc
    27841 tttatccatg tatttttcct cctacgcttc tagcgcttga ttaatttttt tgattaattt
    27901 ttttctttct tcgacgtttt tttctaattc atcacgactt cttaatgcag cttctgtatc
    27961 ttcgtatgct ggcaaaggca caatagaaac ctcgtataat tccacttcat ggattgttcg
    28021 aagcattggt tcagaattgt agtcccacgt ttcttcagtc ggcacaaaac caaaactaca
    28081 ttggttgatg tctcctcgtt ccatagattt aactaagtcc cttgctaccg ttgtgtccgg
    28141 taaatcaact tcgaatttca aaccacgttc atcttcttca aggcgaaggg ttccgctttt
    28201 agtacgtcct agcacattgg accaatcgtg attgaacaaa caacgaacat ccgaattact
    28261 aattgccttt gagaatgcac caggaacaat cacttcgctt aagtcatccc ataaaagtgt
    28321 ttgactgttg aatacagcag cgtagccgct aatggtcctc gtatgagttt cttcatcgga
    28381 acgtgttgaa agattggtga tgtcaatcgt gcgaatttcc ttcttcttca tttccatcac
    28441 ctcctttcaa gtcttgatca ttcgttggta aggaatcatc tgttgcattt ttctggccaa
    28501 tcctagataa gtcatttgaa atatagatag cttgtgtttc tggagtattc tgtttaggaa
    28561 aaccaagcat ttctgccacg ttatctggac ttgtaatccc ggtacgaacg atgttgtagc
    28621 caatatttgt ttttgttgag taaggaacaa aatccaaaat attaattttc cattccactc
    28681 tatagccaga attaggcata aaaaaaagag cggtgtaatg ttcgctcttg tttttcaata
    28741 ttggtttgat tgctttgttg tgcagataca tcatcgcttt ttcaatatct gtcttcatca
    28801 gcgattgata cgtatcaaca ttgattccta gaaattttcc taagtctttt ttgtaaacac
    28861 ctaaataatt caaaattgcc gcgtcatcaa caggactttt taatgtctcg atggaatatc
    28921 cttttcccaa aggaatcatc ttaacagaat gatcactctc attttgcgtt ccttccagtt
    28981 gatccaatat agctttgaca atttttgttt gggcgctatt atttggattg atgtgggcgt
    29041 ctagtttaag catgaacgca agtaaaccgc ctttagtata tttgtccgtc aaaacttttt
    29101 cagcgctcag aacgccttct agagtgtttc ttgcaagatc aattattcca gcacctttta
    29161 atgaatcagt tccgatgttc ttaatgtgtc gaatcatttg accaggtatt ttttgaccat
    29221 tcatttcaaa ttcttcttga agtcgttcat tgattttagt tgttacaccg tacgccaaat
    29281 gaagctgatc cctatccgtt aatgggaatg tctcaccatt gatcaataaa gtatttgttt
    29341 ccaatttggt aaattcgaat cctgtcaaat aatcattggg actcttcaaa atatttagta
    29401 agtgatgatc tttgacttcg ttgccgtcgg gaccaataac aattggtgtg gccaatgcca
    29461 cctgattaga tatgtcttga actaattcat aaacatcaga agattccatg atagatgaat
    29521 ccgtcacata tctttgacca taacgtgtgt agtgtccaaa aatatcctcg atatatccac
    29581 gtttttccat aaaagaatag actgcattcg ataaccgatc acgtaatttc aatttttttc
    29641 accgcctttc tatttatcta tagatggaac ttaggtaatc atctaactca tctgaatcaa
    29701 tatctgtcat ttgattcatc gtttccttat gaccacacaa aaacgccacg aacccatcaa
    29761 tctttttctt tgattgacgt ttacttggcg ctttttgtcc attgatgtta gtaattgcta
    29821 caacgttcaa agtgcaataa aggaataatg gattatcaaa ttgaattcgt ttctcataaa
    29881 acaaccgttc gacatcgtca aaaggagcat tcaatacttt aggatattga gcaacttcaa
    29941 cgcattccaa tcctaagttt tctaatttct caacaagttt gtcactcatc gctggatcat
    30001 aattcacttg ctgaatatca tataaattca tgcagtcctc tatgtactgc aaaatctgat
    30061 cttgatcaat catttttccg tcgcaaaatt caacgaaacc ttgttcagcc aaatcactgt
    30121 aaggaacatt atcttctttt tctcgaaact ctaaatcttc attcggaata aaataaagtt
    30181 gctttacttt aagaaccgct ttaccatctt catcccatgt tgggaagttt aaagatacac
    30241 aggtcaaatc tcgtgtacgt gataaatcta aaccgatata acatggttcg ccacttaaat
    30301 caccaagttc ttgtgtggta actaaacatg gctctacttg atcctgttca aagaaattat
    30361 ccgcgccgtt cacaaataca tccaagtgtt tcgttagaaa ttcagctttg gaatgagctg
    30421 accgttgcgc tgttttaaat gccgattcta aagcagataa atcgactgat attccccagt
    30481 taggattgca catttcccag acttttctat ccgtccaatc ataattttta tttggctcat
    30541 agattagaac aaagtttgaa tcattatcat cacgtttcaa cacttctttt gcttcgcgat
    30601 atacacgcat tccaacagac gacgaacctt tcccagcagt tgagatatta aacatcaatg
    30661 gctgtggtaa agatatctga gcagacttaa aattgtcata ctgttccatt ttctcctgtt
    30721 tatgcaattc atcgttcaat acaaaatatg gattggatcc ctcgatatta tcaatattct
    30781 tggtttgaac aataaattta tttgagtaag ccatatcttc atgaatatag tcataagtaa
    30841 tactagaaac agttcccttt ggccccctaa atattttagt tccttcaagt aggattggat
    30901 tgtttaagat tgtaactgca aaaggtttgg cagcatattg cgcttgagca aaatcagatg
    30961 cgcaagcata gcaatcaact gataacgccc cttctccgta cattgcatat cctaatgaac
    31021 cgacagcaat caatgtttta ccattctttt ttggaatctg gacataagcc tcacgagtaa
    31081 cgcgtacaat ttggcctttt tcattttctt taacccaacc ataaatccaa gaataaataa
    31141 atttttccca gggttctaaa agaaacggtt ttcccaccat ttctcctttt gtgtgaacaa
    31201 taaatgattc tacccaatcc atcatttcat tcgcgcgatc tacatcaaac caaatatctt
    31261 ttcttttttt ccactgatac cagcgatcta ttgctaaacg aacggtctta ggatacttct
    31321 taggtttctt tcttacttct tttgcaaata aatcagcata atttacacca ggttcaatca
    31381 tttttcagca cctgccttct tacgccattt atttctgtgc gctgctagtt catctactgg
    31441 ctttacttct gggcgtgtaa tttcttcatc ttttcttgcg gtcgatcctc cagcaattag
    31501 tctaccagtt tttgctttgt ttgttaatcc taataaatct agagctttca tcttcttatc
    31561 tgcccaagtt tctacttgtt gtgccaatgg atgttttgat tgattagtag ctcctgattt
    31621 attcgtgaat ttttgcgtct ccggaaatcc tttttccttc cacaataaat atttgtgttg
    31681 gtaaatttca aaaatatcca agtatgattc gattaatgga tcaagagtga tggtgtacaa
    31741 atcagacaaa ttcattattt ctaagatacg agctttttca gcacttactt tttcatcaat
    31801 aatcgcttta cgttgcgctt tagtcgtcat ttttgtatac accccccttt gttttttgaa
    31861 aaatttgacc taacgatgcg cgtgactccc cgctacccta tctccccacg cgaaaaaatt
    31921 ttgaaaatga ataggggggc ttcaattgaa atacgaagga aaaacttttt tgtcttccgt
    31981 ttcgttttca acaattggat gacattttga acataatagc atgagattgt ttggatcaag
    32041 cttaagcagt tcgttgtctt tgattggtac aatgtgatgg acgtgtgctc tctttccaaa
    32101 tatgaactga ccacaccgct gacaatgacc accttctctt tcataaataa attgacgcat
    32161 atctttccat gcttgcgttc gatagaatgg tttgttctca tgatgataaa cagactttgc
    32221 ttgctgcttc ttcttgcgtg atctgcttga tctcttgtgt tcagtacagt agataccctt
    32281 tgctattttg ttcgtgcatc cgtcaaactg acagtatttc attctacttc acgaataaga
    32341 tttatgatat cgccttttgc acggatagca ccaggaatat caataccatg tttcttagca
    32401 tatgcacgca attcttttgc agtcatattg tccagttcat ccgtatcttc agtggactga
    32461 tcattagtag cttcgtctcc atcaaaatta gcagcagtgt ttccatcgct atcaagaatt
    32521 tcactgctat gagctatgag ttcgccattg acagcaacaa atgatttgcc atcactcaaa
    32581 attccttttt cttcttttac ttcaaaattt ggttcttgat ctttcggaac aactactgtc
    32641 cttttctttt ctgaatccca atactctgtt ccactaatgg atgttctaat tttgatcatt
    32701 gccattttaa ttctctcctt tgtaatttgt acggattact tttgcgccca ttcgctcata
    32761 ccattcaatc gtttcctgca gattgggtag agtgtgtgac aataaagaaa taactaacgt
    32821 attcattcca gttctgtaat catcattctc cacttgcaca tgtgcatgat taccattcca
    32881 attttctctg tacggactat tctctgcttc aattgctttc ttataaactt tagccaaact
    32941 tgattctact tcaatactaa gtaccgcttc atgaaaatca ttcatttgtt tactctccta
    33001 tcagatattt tgtggtatga tttataaaaa agatgcgagg tgttttaatg cttcaagaag
    33061 ttaatattaa gtatcgtgat cgtcaaaata cagagacaat ttatttgcca aatagttgtc
    33121 cgcattgcgg aaatataatg actccgtatg tgtattttgg ggtttcatca tatagtgcgt
    33181 tcgataatga tagaatcttt ggcgtattag tacagtgtat aaatgatgag tgcaacaaat
    33241 tttatagttt acaatttata aatacgttta atgaagatga aggatattct gaaaatattt
    33301 taattaaaga ttattaccgt ccgccgatta aaatagattt accagaaaat attgaaaaag
    33361 tttctcctac atttgttgat atttatactc aagccacaca agctaaatct gagcaattgg
    33421 aacaaattgc tggagttggt tatagaaaag ctctagaatt tctaattaaa gattatgcaa
    33481 tatctaaaaa ttcagaggat gaagataaaa taaaaaaaat gttattaggt aaaacaattg
    33541 aaaattattt gaatgatttt cctaaactcc aaagattagc caaagctgct acttggattg
    33601 gtaatgatga aacgcactat gttcgtagac atactaataa agatgttcat gatatggatg
    33661 catttattaa atcagcagcg caatttatcg cggctgatta tgatgctgat ttagctgatc
    33721 aatttataaa tggaaataac tttatgccaa ctactaatta aaagaataaa agagccacag
    33781 ataccaatta ataaaacggt tgggatatct tggctctttc atattttttt gacactaata
    33841 gaatatcatg ctgctaacaa tgtttacata gctacaatga ccgaacattt agtgaacagt
    33901 gtaattttag aataaagaga gttgttcttt gtacttctcg ctattgtaaa aatcaatttc
    33961 gttttctttt ttcaatcggt cagcttgtct gtgttcatac tcatctaaaa agttcaatgt
    34021 ctttctaatt tctgcatgtc gctgtctgac atacgatgag ctatacccaa cagattcagc
    34081 aatatcttct aagctcattt gatcaacata tttcatctta acaatttgat tatcgatacc
    34141 agaaaagcta tctatcagca ataacatttc ttctttttgc tcgatcaaca attccaattc
    34201 attttctatc ttttggatat tctcttctaa agaagaagtt ctcgaattct tttcgatgcg
    34261 gacgtttgct aaatcaccac agacccatcg attcaattca agcttacttt tattaagatt
    34321 ccactttaag taaagaatct gctcatctag ttcttggtaa tcttttaacc aatgaaatct
    34381 cacaaacgcc accccttatg gtagaatagt attgcgctgc tatctcgaaa ggggtagctt
    34441 ttttattttt tcttagccgt tccattctgg attgcctttt gttccaaacg tcgttttttc
    34501 ttcttaatct ttgattttgt ttttcccata caattctcct ttccgaaaac acattttgtt
    34561 cgatttttaa tactaaataa aagttggcca gaatttttcg ttcgtcttta ctcatctttt
    34621 tttgaataag attactagca taaaaatagt tagtaccacg aaaatgaaca ggtaaatcaa
    34681 ttacgttcac cttcttcatg ccagttatca gccactagaa tctttgcgtt agtgccgcca
    34741 ccgttttctt ttttgtattt ttcaaatgct ttataagcag ctgtttttga tttatagatt
    34801 tttccggaac cgactgggaa catccatgtg ccctctgtga tagctaagat gtatactttc
    34861 atttaattca cccctttaat ccccaacact acatatccgg acttctgcat gtaatcagta
    34921 atataagtga tctctgcttt gtatgagtca ccagtgtaac taaatgttgg tctcgttagt
    34981 ccaggtaatt catgtttaat gtatttttta tattcacgta gtattaactg atcaccaact
    35041 ttgaagtcac gatcattctt gcggatctcg aattgtttgc gtccgctcgt cactgcttca
    35101 aaatattctg gtagtatttt tagttcgtgg atcattctgt ttcctccttc aaaattcgct
    35161 catcatcaaa aagtgaatct ccacaaaatc cttctcccaa atatttatcg ttcatgtata
    35221 agtcaaccaa atatttgtga tctgcttctc gatattcatt aatattaacg atctttattt
    35281 tgtaaacatt accttcgtcg ctcactatct tgaattgttc tctgactttc accaccgttc
    35341 ctcctctttt tgaaaaaatt ttagtgtttt tgtaagaccc cctaaatcag cttttttatt
//
LOCUS       DAJSKS010000010        35013 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJSKS010000010 DAJSKS010000000
VERSION     DAJSKS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.010"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(134..262)
                     /locus_tag="ON396_000487"
     CDS             complement(134..262)
                     /locus_tag="ON396_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR3112620.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(265..1638)
                     /gene="rlmD"
                     /locus_tag="ON396_000488"
     CDS             complement(265..1638)
                     /gene="rlmD"
                     /locus_tag="ON396_000488"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR3112621.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1749..2804)
                     /locus_tag="ON396_000489"
     CDS             complement(1749..2804)
                     /locus_tag="ON396_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR3112622.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2814..4244)
                     /gene="gatB"
                     /locus_tag="ON396_000490"
     CDS             complement(2814..4244)
                     /gene="gatB"
                     /locus_tag="ON396_000490"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCR3112623.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4241..5713)
                     /gene="gatA"
                     /locus_tag="ON396_000491"
     CDS             complement(4241..5713)
                     /gene="gatA"
                     /locus_tag="ON396_000491"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCR3112624.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5713..6018)
                     /gene="gatC"
                     /locus_tag="ON396_000492"
     CDS             complement(5713..6018)
                     /gene="gatC"
                     /locus_tag="ON396_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCR3112625.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6124..8160)
                     /gene="ligA"
                     /locus_tag="ON396_000493"
     CDS             complement(6124..8160)
                     /gene="ligA"
                     /locus_tag="ON396_000493"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR3112626.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8177..10411)
                     /gene="pcrA"
                     /locus_tag="ON396_000494"
     CDS             complement(8177..10411)
                     /gene="pcrA"
                     /locus_tag="ON396_000494"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCR3112627.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10566..12125
                     /locus_tag="ON396_000495"
     CDS             10566..12125
                     /locus_tag="ON396_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HCR3112628.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12236..14167)
                     /locus_tag="ON396_000496"
     CDS             complement(12236..14167)
                     /locus_tag="ON396_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3112629.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14185..15099)
                     /gene="pfkB"
                     /locus_tag="ON396_000497"
     CDS             complement(14185..15099)
                     /gene="pfkB"
                     /locus_tag="ON396_000497"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR3112630.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15096..15848)
                     /locus_tag="ON396_000498"
     CDS             complement(15096..15848)
                     /locus_tag="ON396_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3112631.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(16007..16216)
                     /locus_tag="ON396_000499"
     CDS             complement(16007..16216)
                     /locus_tag="ON396_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112632.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16227..17555)
                     /locus_tag="ON396_000500"
     CDS             complement(16227..17555)
                     /locus_tag="ON396_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112633.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17691..17846
                     /locus_tag="ON396_000501"
     CDS             17691..17846
                     /locus_tag="ON396_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112634.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17920..18507)
                     /locus_tag="ON396_000502"
     CDS             complement(17920..18507)
                     /locus_tag="ON396_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR3112635.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18590..19840)
                     /gene="clpX"
                     /locus_tag="ON396_000503"
     CDS             complement(18590..19840)
                     /gene="clpX"
                     /locus_tag="ON396_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCR3112636.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20087..21376)
                     /locus_tag="ON396_000504"
     CDS             complement(20087..21376)
                     /locus_tag="ON396_000504"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR3112637.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21530..22462)
                     /locus_tag="ON396_000505"
     CDS             complement(21530..22462)
                     /locus_tag="ON396_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112638.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22619..23278)
                     /locus_tag="ON396_000506"
     CDS             complement(22619..23278)
                     /locus_tag="ON396_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR3112639.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23281..24306)
                     /gene="sppA"
                     /locus_tag="ON396_000507"
     CDS             complement(23281..24306)
                     /gene="sppA"
                     /locus_tag="ON396_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR3112640.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24319..24609)
                     /locus_tag="ON396_000508"
     CDS             complement(24319..24609)
                     /locus_tag="ON396_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112641.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            25018..26823
                     /gene="glmS"
                     /locus_tag="ON396_000509"
     CDS             25018..26823
                     /gene="glmS"
                     /locus_tag="ON396_000509"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR3112642.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26895..28250)
                     /locus_tag="ON396_000510"
     CDS             complement(26895..28250)
                     /locus_tag="ON396_000510"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR3112643.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28274..29443)
                     /locus_tag="ON396_000511"
     CDS             complement(28274..29443)
                     /locus_tag="ON396_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112644.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29440..30327)
                     /locus_tag="ON396_000512"
     CDS             complement(29440..30327)
                     /locus_tag="ON396_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCR3112645.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30516..34208)
                     /gene="nifJ"
                     /locus_tag="ON396_000513"
     CDS             complement(30516..34208)
                     /gene="nifJ"
                     /locus_tag="ON396_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR3112646.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            34407..34928
                     /locus_tag="ON396_000514"
     CDS             34407..34928
                     /locus_tag="ON396_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCR3112647.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         9449 a         7604 c         6307 g        11653 t
ORIGIN      
        1 tcattctgac ccagatttga ttttgcacaa ctcaacagtc cttattggca aatcgccaaa
       61 ataatgtaga ctaactaaaa agactaagaa acaataacaa aagttaacac ttcttagtct
      121 ttctttagca cgtctattta ttaaagattt tagggccttt tctcatgttt ttacgatcat
      181 tcttaggtgt ttccatttgt ttggatcggc ctccaccttg tttctttttg atcaattctt
      241 tcattttttc tttttggtcc atttttattc tcctttaacg aatgaagcga ctaattcgat
      301 atgatgggtt tgcgggaaaa gatctacagg ctgaactttc ttcacggcat aacctgcttc
      361 tgcaaataac ttagcatctc ttgcaaaagt tgccggatta catgagatat agacgattcg
      421 ttctggtgac atttcgattg ctgattcaat aaaatgatga tctaatcctt ttcgtggagg
      481 gtcaactacg atcacagatg gtttgatccc tttttcttgc cattttttca taactttttc
      541 tgcttttccg acttcataat gaacattttc gattttatta tttaaagcat tgaactgtgc
      601 atcttcaatt gcttctggaa taacttccat cccatacacc ttatccactc tgtctgctaa
      661 agacaagcca atcgtaccta ttcctgaata agcgtcgatc acaatgtctt ttttatctaa
      721 ctcggcaaac tcgatggctg tttggtacag atactctgct tgttgagtat ttacttgata
      781 aaatgacttt gctgagattc gataggtttt tcctaatagc tgatcttcga tatagtctct
      841 gccatataaa actttttctt tgtctcctaa aatcacattt gttttttctt cattgatatt
      901 ttggatgacc gaaaccactt ctggcaattc ttcatgaata gcttcagcaa tttccttgga
      961 tttaaagaat ttttctttac gagtaactaa aacgaccatc atttcatggg aataatgacc
     1021 acgacggata atgatgtgac gaagctgtcc ttcatttttt tcttcattat atcctcgtac
     1081 ttggaaccgt tgcaagatat ctctgactac tttgattgct tgatcgatga caggatcttg
     1141 gatataaaaa tcttcgatag ggactagatc atggctattc tttttgtaaa aaccggtggc
     1201 taactgtcca tcgatacggc gaacagggat ctgtgcttta ttacggtagc cgaatggttc
     1261 tgccattccg accgttggaa gtacaggaac ttctggcatc ttagcaatct tcgccattac
     1321 atgctctact tgtttttgtt tgaagacaag ctgttgttca tacttcaaat gggctaaagg
     1381 tgcgatacct atgcgaagta atgcattgtt ttctacaggc tgacgatcag gtgattgtgt
     1441 aataaatgtt tcgacttttc catatccaaa cttattgcct gcttttacta ctagtatttc
     1501 tacttcttct ccagggagtg cattttcgat aaataatgga taaccatcga tttttgctac
     1561 ccccattcct tcataactca agtcgacgat ttcgactgta tatcgttcgt ttttctttac
     1621 tatttgctgt tctgtcatat tttgctcctt taagcctatt tttcaatcct actgaactct
     1681 actataaatc agctaattac acaactaaaa ccggcactga ccttactacg ttcagcaccg
     1741 gcttttgatt aattcttttg attaggtgtt tcgtctgcta tttttccgtc accgtcgata
     1801 tcttcatccg ttaaacgttc gacttctttt acgaattctt tgctgacaac ctctaattct
     1861 tcttcatctg tacctgtgat tgcttcatca ggaatctgat cggtattggc aaagaactca
     1921 atgtgttgat gaagattttg gaaatgcatc ggtgcttccc cgccgtattc accatctaag
     1981 ttgatcatta ctggtcgatt gatatcatct tctgtttcaa cgaacaaact gcttgttttg
     2041 gtatagatta ttcttggatc atcgacatgt cgaccgccat tcagcatgag tgctgcaata
     2101 tgcaagatct caaagatatt tgctgtcttc acaatgatta aagaaaactt cccatcatcc
     2161 aatttggcat ctggcgcgat ctgttcgaat cctccaacag agttcgtcag ccctaggaaa
     2221 aacatcgaag catttccgat atattcccct tcatcatact tcaagcgcat tttgattggc
     2281 tttactcgag ggagcatctc tgctcctttc gcaagataag caagataacc aaaaatactc
     2341 tttagttctg atggaacttc ataagtcaat tctgtcaaat agccacctgc agcgatattg
     2401 atgaagtaac tgttatcaga aaggccgatg tccattttta cggtttggtg tttttttatc
     2461 acttctgctg ctgctttaat attatctcta ggaattttta atgctcgagc atagtcgttc
     2521 gttgtaccag ctgggatgat ggccatttta ggacggtgtt caagcggagc gattccatta
     2581 accacttcgt tgattgtgcc atcgccacca gctgccacta ctagatcaaa acctagttct
     2641 gcgactctac atgcttcatt cttggctgaa tctggttctg gtgtagttgc ataggcgctg
     2701 gcttcaaatc ctgcttcttc taatgcctgt aggatatccg ccaaattttt tttcaataat
     2761 tcttttcccg aggtggggtt ataaattacg cgtgcttttt tcattttcag cctctatcgt
     2821 ttcgctaatt catctttaag tagcttattc acgacaccag gatttgcttg tcctttagtt
     2881 gctttcatga tttgaccgac taggaagcca accgcacgat cttttccatt tttgaaatca
     2941 tcgacagatt gttggttgtt atccaaaatt tcattaatga ttggcagtag tttgttcgga
     3001 tcagataatt gcacccaacc atttttttca actacttctt gggcttctcc gccatttgtg
     3061 atcaattcac ggaagacttt tttggcgatt tttgagctga tcgttccatc ggcgatcaaa
     3121 cgaatcattc ctgctaagtt ctcaggtgtc aattttgtct cagctaattc cagtttctca
     3181 ctgttcaagt aagcagaaac ttcacccatc aaccagttag atgcttgctt ggcatctgca
     3241 ccattttcca aaacagcttc aaaaaaatca gacatttctt ttgtaagtgt caataccatt
     3301 gcgtcatact ctggaagtcc aaactcacgg atatagcgtt cgcgacgaga agctggcatc
     3361 tcaggcaaac tagcttttac tttttgaatc cattcgtcat cgatcacgaa tttcgggata
     3421 tcaggctctg ggaagtaacg ataatcacta gacccttctt tgacacgcat caaaactgtt
     3481 ttattcgttg tttcgtcaaa acggcgtgtt tcttgttgga tttctcctcc tgaaagaaga
     3541 acttttgctt gacgtttttc ttcaaaagct aaaccttttt tcacgaaaga taaagagttc
     3601 aagtttttca attctgcttt cgtaccgaac tcttcttgtc cataagggcg aagagaaata
     3661 ttggcatcac agcgcataga gccttcttcc attttcacat cgctgacttc tgtaaattgg
     3721 ataattgatc gcaatgcttc tagataagcg tatgcttctt ctggtgaacg catatcagct
     3781 tcagaaacga tttcgatcaa aggcgtgcct tgtcggttca aatcaacgta agaataaccg
     3841 ccaataccat gcatgttttt tccagcatct tcttctaaat ggacacgttc gatccggatt
     3901 tttttctttt taccttctac ttcgatttcg atccatccgt catgaccgat tggttgatca
     3961 aattgggaaa tttgatatgc ttttggatta tctgggtaaa agtaattttt ccggtcaaaa
     4021 tgagtatctt tagagatttc acagtttaat gccaaagctg ctttcatccc gaattccagc
     4081 gcagctttat tcattactgg cagcacgcct ggataacccc agtcgatcac gtttgtattg
     4141 ctgtttggtt ctgcaccaaa atgagctggc gctggagaga aaatttttga gttggttttt
     4201 aactcgacgt ggacttctag tccaatgact gtttcaaaat tcattcgtct ttccctccta
     4261 aaatagctgg ttgttgttta tggaattctg ttgcttgttc aaacgcatat gccgcttgat
     4321 acatcgtttt ttcatcaaaa taattaccga tgatctgcaa tccaactggt aatccttctg
     4381 ataaacctgc cggtacagac atacctggaa gtccagccaa gttgacaggg atcgttaaga
     4441 tgtcactcat gtacattgtg attggatcgt taatgttctc accgattccg aatgcaactg
     4501 ttggtgaaga tggcccgatg atcaagtcat aatctgcaaa gactttttca aaatctcgtt
     4561 tgatcaatgt acggacttgt cctgcttttt tgaagtaagc gtcataataa cccgcgctta
     4621 aggagaatgt acctaacatg atacggcgtt ttacttcttc accgaatcct tctgaacgtg
     4681 agttgacata tacatcatct aatgatttca cattttcaga gcgataaccg tagcggatac
     4741 catcgaatcg ttgcaaattg gaacttgctt ctgatgaggc aatgatataa taaacagcca
     4801 caccgtattt tgaatgaggc aagctgactt cttccacaac agcacctaat gaacggaatg
     4861 tttctgctgc tttcaataca gcttctttta cgccaggggc cacaccttcg cctaagtatt
     4921 ctttaggtaa agcaatcttc attcctttga tgtcgcctgt cagtccctct gcaaaatcag
     4981 gaactgaaac gcctgaagaa gtaccgtctt tttcgtcata accgctgata gctgtcaatg
     5041 ctaatgcatt atctttcacc gtacgggtca ttggtccgat ttggtcaaga gatgaagcaa
     5101 aggcaatcaa accaaaacgt gagacacggc cataagtagg tttcatccct acgatgccgt
     5161 taaaagctgc tggttggcgg atacttcccc cagtatcact acctaaagaa actggaattt
     5221 gtccagctgc aactgctgct gcagaaccac cagaagagcc accaggtact tttgttgggt
     5281 cccaagcatt ttttgttttc ttgaaataag aagtttctgt acttccaccc atagcaaatt
     5341 catccatgtt tagcttacca acagggatca tatctgcttg atagatttta tccatgaccg
     5401 ttgcatcata aattggttca aaattgtaaa ggatcttaga tgcagcggtt gtcaagatat
     5461 cttttgttac gatattatcc ttgataccaa ttggaatacc agcaaggacg ttgctttcat
     5521 caatcccttt ttcatctaaa gcttttgcct gttccaatgc tttttcttcg ttcaatgtaa
     5581 tgaatgcctc gacctcaggc tctgtttctt tgattcgttc aaatgttgtt tttgtcaaat
     5641 cttgtacagt gatttctttc gatacaagca tatgatgcag ttcttctaga gaacgattgt
     5701 gtaattctgt cattatgcgc cagcctcccc attatcgatg attgcgggta ctttgataaa
     5761 gccgtcttct gtttccggca catttttcaa taattcatcg cggttccatc cagcttgcgc
     5821 ccgatcttgt cgcatcacgt ttactgtttc aaccacattc gacgtaaatg gaacaccttc
     5881 cgtatctact tcttccagca tttcaaccat atcgatgatt ttacctaatt ggtcggtaaa
     5941 acttgcaagt tcttcatctg cgaacgcaag ttttgctaat ttggcaacgt gtttcacttg
     6001 ttcttcacta atcgccatac taccccttct ttctaaacga gttaaaaact ttgctctcta
     6061 ttttaacaca agagtgccgt gtctgaaatt gtttattgaa atttgtctga aaaaactcat
     6121 ttttcattct tctactgagc gacttttagc caatgcatct gccatttgat cttctgtcca
     6181 aacttcgatt cctaattctt gtgctttggt cagcttgctg ccagcatctt ctcctgctac
     6241 cacgatatcc gtttttttcg atacgctacc agtcactttc ccgccaagat tttcgatcgt
     6301 ctcttttgct tcttcacgag taaatcgtgt tagtttacca gtaaggacta ctgttttttc
     6361 tttaaaagga gactcgactt cttgcaattg ggcatttcga agccctttgt attcgaaatt
     6421 gacgcctgct tgttttaatt cattcatcaa ttcatggact tcttcgttac tgaaataagt
     6481 gactacactg tcggcaatga tatcaccgat cgtatcaagc gcaataatcg actcatagtc
     6541 acttttactc aatgtttcta gatcaccgaa atgttctgct aaaatttttg ctgctttcgc
     6601 tccgacatgt cggattccta acccgaaaat caaccgctcg accgaattat ctttgctgtt
     6661 gtcgattgct gtcaaaatat tatttgctga tttttctttt attttgtcca acgtcaataa
     6721 ctcttcttct gtcaatttgt aaagatcagc aacatctgct actagttttt tatcatacat
     6781 ctgttctagt acgcgcggcc ccaacccatc gatattcatc gcatttcttg aaacaaagtg
     6841 gttcaaacct tctttcattt gagcagggca tttaggattg atgcaccgta aggctacttc
     6901 ttcatccaaa tggacaagtt cacttccaca aactggacaa tgtgtaggca attggtattc
     6961 ttcgctgtct ttaggtcgtt tatctaacac gacttgcgaa acttctggga tgatgtctcc
     7021 cgctttataa atcagaactg tatctttcaa gcggatatcc ttcaacttaa tataatcacc
     7081 gttatgcagg ctggcacggc ttactgtcgt tcctgctact cggactggtg tcatgatagc
     7141 tgttggcgtt actacccctg ttcgaccgac tgtccattcg atgttttcga ttagcgtttc
     7201 aacttcttct ggcggaaact tgtaagctgc tgcccatctc ggtgctttga tagtaaaacc
     7261 aagttgatct tgtagcgaga attcattcac tttgatgacg attccatcga tttcataggg
     7321 caaatccaca cgtttgtcat gatattcttc aatgtatgac cacacttcat caatcgaatg
     7381 acatagacgt ttttcccgat tcgtgtggaa tccaattttt tctagctctt ctagcgcatc
     7441 atactgggtt tttgcttgca taggaccgaa atcagctact gtgtataaaa aagtgtccag
     7501 atttcttttc gctgtgattt tagaatctaa ctgacgcaaa gaaccagctg cggcgttccg
     7561 aggattcgca aagatatctt ttccctctgc ttctctgtct tggttcaatt ggacaaatga
     7621 ccgttttggc atgaaacatt cgcctcttac ttcgatgttc attggttctt ttaatttgat
     7681 tgggaccgac cggacagttt ttaggttttc agtgatgttt tctcctacag ttccatcccc
     7741 gcgagtcgca cctctcacga aatttccatc ttcataacgc aaagaaacgg aaagtccatc
     7801 tatttttagt tcacagcaat agtcaactac acgtcccact gctttttgta cacgttgatc
     7861 aaaagctatc agctcttctt tgctgaaaac gtcgttcaaa ctgtaaagcg gaatatcatg
     7921 agtgactttt tcaaaacctt ctaatacttt tccgcctact cgttgagtgg gagaatcaga
     7981 cgtgatcaaa tcaggatatt gttcctcgat tgctactaat tctgcgtatt ctttatcata
     8041 aacatagtct tccactgttg gtttatcttc tacgtagtat tcgcgagacc attggttcag
     8101 tcttgtacga agttcttctg ctcgtttagc agcttcgtca atcgtcattt catgttccat
     8161 catttaccct ccactactaa gctttttcaa ttggcgcaaa ggcagccaaa agacgtttta
     8221 ctccttgact tgggaaagcc acatctagtt ctagatcctt cgacgtcccg cttactcgga
     8281 cgactgttcc tagaccccat tttttgtgtt tgactttatc tccaggtttc cagctgttat
     8341 tttcccctcc agtagcactt ttagagggaa caaccgctgt ctctggctgt ttatagctat
     8401 atgctgtttt tttagcgaat acaggcgttt ttttaggcgt aggacgcatg ccttcgattt
     8461 ccagtaactc ctgatcgatt tcttctacaa aacgagaagg tcggttatat tgcgtccgtc
     8521 catataatgt tctggagaaa gcatttgtta aatacaacgc ttcttctgct cgcgtgatcc
     8581 ccacatatgc gagacggcgt tcttcctcca gttcgctttc ttccatcaat gctcgagata
     8641 aaggaaaaac cccctcttct agaccaatca ggaatacaac tggaaattct aatcctttgg
     8701 ctgcatgcaa agtcatcaat gtcacttggg aagcatcttc ttctaaattg tcaatgtcgg
     8761 aaacaagtgc cagatcattt aagaagaccg ttagtttttc ttctggtgca tcagcgtctt
     8821 cttcattttg ctgttcgaat tgtttgtcga attcttgtgt gaccgttaaa aattcctcca
     8881 agttttcgat gcgtgcttgt gcttctaatg tattttggat ttttaagtct tctagatatc
     8941 cgctacgatc caaaacctct ttcgtcaatt ctgtcactgt caagtatggg atcatctgag
     9001 tgacttcttg gatcatctct ccaaatgcac ctaattgctg tcctgctttt ccaccgatat
     9061 ttgccagatc cacattttgc gctgcttcaa gcagtggcca ttcatgaaga gaagcaaaac
     9121 tgcgtagttt ttcgatagat cctggtccga tgcctcgttt gggcgaatta acgatacgct
     9181 caaaactgat tgaatcttga ggatttacca gcacattcaa atacgctaaa atatctttga
     9241 tttctttacg atcgtagaac ttgtgccctc caaccatttt ataaggaata tttgctttca
     9301 acagagtttc ttccattaca cgagattgtg cgttcgtccg gtacaagatg gcaaagtcgc
     9361 cgtaattccg atgattgctt cgaatctctt cttgcatccg gtctacgata aaacgggttt
     9421 cgtctcgttc attatctgca cggtagtaag tgatcttgtt tccttctttg ttttctgtcc
     9481 acaagttttt tggttttcga ttgctattgt tttcaatgac ttggtttgct gcgctaagga
     9541 tgtttttcgt ggagcgatag ttttgttcta gcaaaattac tgcagcatct ggataatctt
     9601 tttcaaaatc caagatgttc tgcatatctg caccgcgcca gccgtaaata ctttgatctg
     9661 catctcccac gacgcacagg ttcctaaatc tacctgccaa taaattgact agcgtatact
     9721 gtgcgtgatt tgtatcttgg tactcatcta catgaatata atggaatttg ttttgataat
     9781 acgtgagtga atcaggatgt tcttcaaata atcgaatcgt attcatgatc aggtcgtcaa
     9841 agtccataca ttgattatat tgaagctctt tttggtaagc agcataacat tttgccgcaa
     9901 tctcttcgta ataactgcct tgcatttttg caaaatcctg aggggtctgc aggctgtttt
     9961 ttgcttgaga gattgttcct aatatacttc gaggatcata ttttttcgga tcaatgttca
    10021 gttcttttag tatccgtttc atcaaggtca gctgttcaga agaatcgatg attgtaaagt
    10081 tccggttata cccgataaaa tcaacatctc ttcttaaaat acgcacacac atcgagtgaa
    10141 aagtcgatac ccagacatct tcgcctccag atgcgagaat cgcattgaca cgttctttca
    10201 tttcttttgc agccttattg gtaaaagtaa tcgctaggat gttccaagga ttgacttctt
    10261 tttcctcaat caaataggca atgcggtgtg tcagcactct cgtctttcca cttcctgcac
    10321 ctgccattag cagtaatggt ccgtcagtgt gaagaacagc ttctttctga cgggggttca
    10381 tcccgtttaa taattcagct ttggatgaca ttaacaacat cctttctttt atcaatcttg
    10441 ttaattatag cattttcttg gctttcatta aactagctag ttcaaaatca tttgtttctt
    10501 tttatggatt tttccttgtc aattcgttga tttcgcgcta aattaaaaga aagcgaggcg
    10561 tttgaatgga aaaaaaagga aaaatcaaga aattagataa gactcagata tattttattc
    10621 taaaaggaat atttgttgga ctgctgacag gaatcgtcgt tagcctgttt cgattatcta
    10681 tcgaaaagct aagcgattta gttcgaagta tctatgaaat gagtcgggaa cagccgattt
    10741 acttaattgg cctagctatt tgctgcatcc ttgcagcatt tttcgtgggt tatctggtca
    10801 aaaatgaacc ggatatcaag ggaagcggga ttccacaagt agtgggacag cttcgcgggg
    10861 aattatcaat gaactggttc tctgttttat ggaaaaagtt cattggcggt gtactttcag
    10921 ttggtgcggg cctttttcta ggtagagaag gaccttctat acagcttggc gcatccattg
    10981 gacaaggagc tggacaattg tttcggagtc catcttcaga agaaaagatt ttaatttcta
    11041 gcggtgcaag tagcggactt gcagcagcat tcaatgctcc tatcgcagga ttactctttg
    11101 ttttagaaga aatccatcac tccttttctc cacttgtttg gctgacatca tttgcttcag
    11161 caatcactgc taacttcgtc tctctttact ttttcggctt gagacctgtt ttatatctag
    11221 gtgaccttcc ttctttgccg ttgaaatatt atggttcctt agtgatctta ggagtacttc
    11281 taggcatctt aggttttatt tatcagaatg tcttgctggc tttgccaaaa ttttacaacc
    11341 gattgccttt accagcttat tttcacgggt tcgttccttt cttgctgatt ttgccaattg
    11401 gttatttatg gcctagtact ctaggcggag gcaatcaaat cgttttagct tttggacatg
    11461 cttccttgcc aatcattatg attatcggtt tgtttgttct tcgattcgtt ttttccatga
    11521 tttcttacgg tgggggtctt ccagggggga ttttccttcc gatattgaca ttgggggctt
    11581 taatcggtag cttttatgga aacattatgg ttgatttcgg gatggatcca atctacatca
    11641 aggattttgt cgttattgcc atggctggtt atttcacagc gattggaaaa gcaccgctga
    11701 cagcaattat tttagtgaca gaaatggttg gctctcttaa tcatctgatg cctttaggat
    11761 tggctgctct tgttgcttat atcgtcaatg accttctcgg cggtaatcca atttacgaat
    11821 cattattgga acgtctactt ggaaatactc agaccgcctc actaaaaggt gcaaggacaa
    11881 cttttgattt tccagtaacg gcagagagtt ctttagacgg attaatggta cgagacttca
    11941 attggcctaa aaatatgctg ctaatctcta tacgcagagg aaatcaggaa atcttgactc
    12001 acggtgatac gatcatgaat gtaggagata ttttggttat cttgactgat gaagcaaact
    12061 tgccaagaat caagcaggaa atcgaggcta aatcttctat cacaaatttg aagcaatcat
    12121 tttaacgaac atggaaaaac tattttacaa caactattca tctacactac ttgattctat
    12181 atgattctat aaaaaaagaa gaggctggga aaacccagcc tcttcttttt tatttctatg
    12241 cttctgctac tgcaggagct tttctcaata aaccataaat cacaccagat acgactgctc
    12301 cgatagcaat aaacaccaaa tacatgaccg ggtgactcag taagaataca acgaaaatcc
    12361 cgccgtgagg agccagtaat ttgattccga aagctccgac taatccgcca gttaaagcag
    12421 aaccaataat aaaacttgga atggcacgaa tcgggtcagc tgctgcaaat ggaatcgatc
    12481 cttccgtgac aaacgatagt cccatcacga tgttcgtcaa accagcatct tgatctgttt
    12541 tgctgaattt attcttaaac aaacgtgtag caacgaaaat tgccaatgga ggtaccattc
    12601 ctccagccat aacagaagcc atcacgacac ttccgccagt tgcaacagtc gtagctaaag
    12661 ttgctgtacc aaacacataa gcggctttgt tgattggacc gcctaaatct gccgccatca
    12721 ttccagctaa taatgcacca agtaaagcag cattcgaacc actcatactt gtcaagaaac
    12781 cgttcaatgc atcattgatt gctttcatag ggacattgac cagcaacatc aagaaaccag
    12841 taatcagtaa gccaaacact ggataaaaga ggatcgtttt aattccatcc aatgattttg
    12901 gtaagttctt gaataattta cgtaaaaata cgatgacata accagctaag aatccaccaa
    12961 tcagtgcacc taagaatcct gcaccgcctg tattagctaa tgcaccagca gcaaatccaa
    13021 cgatcaaacc agggcggtca ccgatacttg aagcaataaa tcctgctaat accggcagca
    13081 tgaaaccaaa tgcagcttgt ccgatttggt tgaaccagct agcagcttga ttgtaattcc
    13141 ctagatttga taattgatca tgtggcacgc caataaattg gtcgatcatg aaagaaagcg
    13201 caatagcgat tcctccacca ataacgaaag gtaacatgtg tgatacgccg ttcatcaaat
    13261 ctttgtaaat tctttgtccg atagtaccat ccgcatttcc ttcttctgaa ctgctttctt
    13321 ttccatcacc atggaatact ggtgcagtac cactaagggc tagattgatc aactcttctg
    13381 gtttacggat accatcactg acaggacgat tgatcagttc ttttccgtca aagcgattca
    13441 tctctacttt tttgtctgct gcaacgatta caccttctgc acgggcgatg tcttcagcag
    13501 tcaatcggtt tttgattcct tctgaaccgt tcgtttcgac tttgatctcc acacccattt
    13561 ctttcgcttt tttcttcaaa gcatcttctg ccatgtaagt gtgagcgatc cctgttggac
    13621 aagctgtaac ggcaacaacg aattttcttt ctggatgagc agaagcttct gcagcttgtg
    13681 tttcttgttc tgctttttct tctgcttctt tttgctcttc agctgtttgg aacaagtctt
    13741 ggacttcttc tggtgtattt gcttctttta attttcctac gaaatctgca tcaatcagca
    13801 atcgagatag tgctgctaat gcttgcaaat gtgtgtcgtt tgcgccttct ggagctgcga
    13861 tcatgaaaaa cagataagtt ggctgtccgt ctaaggcttc ataatctact cctttttggc
    13921 tttttgcgaa taaaactgtt gcttctttaa ctgcactgtt tttcgcgtga ggcatggcga
    13981 ttccatcacc aagacctgta gaagtttgtg cttcgcgggc tagaattcct tctttatatg
    14041 tttcgatatc ggaaatccgg ccggcatcaa acattttttg gaccatttca tcgattgcgc
    14101 cttttttatc agtcgcttgc aaatccatga tcatcgcttc tttgaccatt aaatccttga
    14161 tattcatgat tatcctcctt gaaactaatc aatttttgtg atttcaactt ctggcagcaa
    14221 ctcatcaatc aattcgcgag ctgccaagtc atcagaaaaa gctgttgcgc ttccgcaagc
    14281 aactccccat ttaaaggctt cgattggatc tttcgtacgg ctgtaagccc caataaagcc
    14341 agcaatcatt gaatcccctg cacctactga atttttcaat gggcgaacta atacattcgt
    14401 tttataagtt ccttgttccg tgaagagcaa agcaccgtct ccagccattg agatcagcac
    14461 attctgtgcc ccttcagcta gtaatttttt cccatacgga atgatttctt ctacagattg
    14521 gaaggttgtg ttgtaaagct cagctaattc gtgattgttt ggtttgatca acaacggttt
    14581 cttaacaagt gcatttttta ggtcatcacc tgttgtatca atcacgaaat ctgcaccttt
    14641 ttctcgaatg atatcaatca gttcttgata aaatcctttt cgcaggctag ctggcgcgct
    14701 tcctgacaat acgacgatat ctccttgtcc tactgttcct aatgcttgct tcaactcatc
    14761 gatttcttca tctgaaatag atgggcctaa gccattgatc tcagtttcag tgtctgattt
    14821 tagtttgata ttgattcttg tatcttcttt tacggttgtg aacaaagact ggatttgttc
    14881 tttttcaagc cacttcgaaa taaattcgcc agtgaaccct cctaaaaacc ctagtgctgt
    14941 agattgagaa tcgatccgtt tcaaaatacg ggaaacattg atcccttttc ctccaggtaa
    15001 cttgaagtct tccttcattc ggttcaaatc gcctagtttt aacccatcta cgcgtacgat
    15061 ataatcaatt gatggattaa gtgttactgt gtaaatcatt gtattgcctc cttaattgtt
    15121 gtcttttcac ggaattcatt aacgaattct aatggacaac gctcggttat gatcgtggct
    15181 gcttccaaag gtgcaacaga ggtgaaggac aactggttga atttggtatg atcaatcaat
    15241 acaaatgtct cttcggaatt tttcatggct aaacgtttaa cagctgcctc ttctggatcc
    15301 ggagtcgtga atccttgttc aagatgggca ccgttcattc ccataaatgc tttattaaaa
    15361 tgaaaatttt ttagttgttc tatcgcatta gatcctaaaa cagcattagt tgccagcttt
    15421 atcgttccac ctaaaataat ggtagcaagc tccaaatcaa ctaaatttgc cgcatgttga
    15481 acggaatttg tcactactgt gatctgtttt ccttgcagaa aaggaatcat ttctaacgta
    15541 gtagagccag cgtctaaata aatgacatct tcgtcattaa tggtttcagc tgccgatttg
    15601 gcgatttgct gtttttcgtg aacgtttttg agtgattttt cactcatatt ctgctcaaaa
    15661 ttcaataatt gctctttctt agctccgcca tgaactcttt ctagaaaccc actattctcc
    15721 agctcttgta aatcacgtct tatcgttgat tcagaagcat tcaaaaggtt tacaagatct
    15781 tgtgatttca cgattttctg ctgttcaagc aagtctagga ttttttgtct gcgttcctct
    15841 gtaagcattt tcacgatcac ctcaatacaa ataatagcat attttttttc tactagcaat
    15901 ataaatcagt caaaaacgtt cataaaaatt ataaatcagt caaaaaaaaa taaaaaacca
    15961 gtcagaaaga ctggtttttt atttttttgt ctatcatcaa gccgttttat cgttttttat
    16021 tttcgcttct tgtttttttc atataaacag aagcaggttt tctttcctta tgaagaagat
    16081 aataataaag tcccatcccg ccaaacatga ccaaaaaacc gatgactgat cgaataggaa
    16141 aattaggaac aaaaaagaga cagacggata ataatgatgc cggaaccatt attcgataat
    16201 agagttgttt atccattttc ttcacctcat attttttgta aagtaatcaa ttgttctaat
    16261 tctcggtaat ctaaaaattg cacacgcaga tgctgctcac ctttcttgac atcgaattgg
    16321 atacttcgcc gctttttctt tattcgtttg aaggtaacat cagagagtct atgtttgatt
    16381 tcctttaacg gtaattctga cagggattct gaaggaacat agattcttaa ggataaaaga
    16441 tactcttgat atttttcatc tgttacctct acttctagat attccctaat ataataacaa
    16501 taagcttcga ttccattatc caaagcaaca gtcggatcga taactttata gcacatcatg
    16561 cgtgatcgat tctcgtcaac ccaaggaaaa tacgcctgag catttagtgt tccgcgaaca
    16621 cgcctttctt tataatctcc ttgttggata tcataacttt ttgttacaaa aaatgaagca
    16681 ttctttttag gaacaggttg catttgtgca ttctgatact ggacagattg gatcgtatgg
    16741 tcagtgtaaa tgtattcaac gaatggcggt ttcaataaac cgacaccatt gtcccaagca
    16801 agatgatcaa acccaagctg gtccctaaaa taggattgaa acatttgatt gtttggcttt
    16861 tgccagttaa aggggtctcg ataaagctgg atacatatga cttcgttttc taattccttc
    16921 actttttcta cttgttggat gtcgagaaaa atcggtttag ggatgatttg atgataatag
    16981 cgactataat acatttccca acacgaagtg aaacataaac cttggtaaaa aagatcgtat
    17041 cctggaaatt ggttgcaatc gactactact tccccgtttt gttgtttcat tttaggtaaa
    17101 tgttcgattt catccttttt ctcaaacaat agccgttgag agatctgttt tgtattgtga
    17161 tagataaact cgctataagc tcgcatatag gcgaacaccc catgagcaat catttttttc
    17221 tgaatgaagt ctcgaatcaa aaattgcttt tctttgaaaa cagagataga caatacattt
    17281 tttatcaata attgattatc ttccaagcga aaactacatt cgttctcgtc atttcctaca
    17341 actaaccaac tttcgtctct taccgatgct agcattattt tttcttgaag ctttcgaaga
    17401 tcatacggat ccgcttgttg tgtgaaagag cggataaatg ttggctggat caacagacgt
    17461 gtcaaaatag cccaatcgct tatttcctgt tttttttctt cttcatttaa gtctgtgagc
    17521 tggatagtca tcaataattt atcttcgcca atcattttct cacctctttc aaatcttttt
    17581 ataatttaat tataaccaat gataaagaaa aactaaaact ttatgtctta gaaaccaatt
    17641 tggacgatta catgatagac taagtaaaaa gaaacaaaga ggtgtttaca atggggttga
    17701 aatttgaacg tacagataat ttagataaac ttttcgaatc atttgctgtc gatccggaaa
    17761 aaacagttgc accaaaagaa gagatcgatc gatttctaaa accacagcca caaaatgaaa
    17821 agaaaaaaga caagaaacaa aagtaagtta agctcaaaaa aagacttacc agccaaactt
    17881 ttatgaaaga gtttgactgg taagtctttt ttattctttt tatgattcaa ttgcttgttc
    17941 gattgctgcc cacgcttctt cttttccctg ttttgtaacg gaagaaaaga gaataaacgt
    18001 gtcattgcga tcaaaatcca acgctttttt gatttcgctc tcatgcttgt tccatttacc
    18061 acgagggatc ttatctgcct tagtggcaac aacaattaca gggatatcat aatacttcaa
    18121 aaattgatac atctggatat cttcttgaga aggcttatga cgcaaatcta ctaaagaaac
    18181 gaccgcacgt agctggtctc tagtcgttaa atatgtctcg atcatctttc cccattttgc
    18241 tcgctcagtt ttggatactt ttgcataccc atatcccgga acatcgacaa aatgtaatgc
    18301 gttctcgatc aaataaaaat tcaatgtctg ggtctttcct ggcttaccgg aagtacgggc
    18361 taaattcttt cgatcaatca atgtgttgat aaaagaagat ttccctacat ttgatcgtcc
    18421 tgccagagct atttctggca attgtgtatc tggatattgt tttggtgcca ctgcacttat
    18481 gataatttca gcttgatgga ctttcatggt ctctctcctt aacaaggtga tgaataaagc
    18541 aagcggctgt cgcaaaagcc aaaatgactt ttacggcagc ctcttttcat caacccgctt
    18601 ttttctctga atctttatag attactgttg gctcaccaga caattcagct gcttcttttg
    18661 tgataatcac tttttcgatg ttttcatttg aagggatatc aaacatcaca tccatcatga
    18721 tttcttcaat gatggaacga agtcctcgag caccggtatt gcgttcgatt gcttttttcg
    18781 caatggctct caacgcttca ggttcgaatt caagttcggt attatctaga gaaagaagtt
    18841 tttgatattg tttgactagc gcatttttcg gctcagtcaa gatccgtacc agatcatctg
    18901 ttgtcaattt ctctaaagct gccataacag gtaatcgtcc gataaactca gggatcaatc
    18961 cgaattttag taaatcttca ggaatgatat gctgcatgac actttcgtct tcagaaagtt
    19021 tttggttttg tgtaccaaat ccaatcgtct tttcgcccat acgattcttc acaatcgttt
    19081 cgatgccatc aaatgcacca cctacgatga acaatatgtt cgtcgtatca atctgaataa
    19141 attcttgatg tggatgttta cgtccgccct gaggtggcac acttgcaaca gttccttcta
    19201 gaattttcag caaggcttgt tggacacctt cgccggaaac gtcacgagta atagatacat
    19261 tttcgctttt acgcgcaatt ttatcgatct catcaatata gatgatccct ttttctgcaa
    19321 gttccacatt gtaatcagca gattgaagca atttaagcaa aatgttttct acatcttccc
    19381 ccacataacc agcttctgtc aaacttgtcg catctgcgat tgcaaaaggt acattcaatg
    19441 tttttgctaa tgtttgtgct aaaaatgttt taccagaccc tgtaggtccg atcaagcaga
    19501 tattgctttt ttgcagttca acatcatctg tgttttcttg tgcttgctga ttgacacgtt
    19561 tatagtgatt gtatacggca acagctaacg agcgttttgc gcgatcttgt ccaatcacgt
    19621 agttattcaa tacttcaaga atttctagtg gtttagggac ttcagtgaat tcgcgtacag
    19681 cttcttcata aaattcttca tcaatgattt ctttacatag gtcgatacat tcattacaga
    19741 tgtagacccc tggaccagct acgatctttt tcacttcttc ttgggttttc ccgcaaaaag
    19801 aacagcgtac ggtctcattg ctacttggat tgtcatacat ggtcttcacc ccttaacttg
    19861 caaaaagtat gcaagaaact atcaaagact ttacagtctt ttactatcat aacatagttt
    19921 agtaaaaaga aaaagtgttt aacaaaggat caactgacct atacctttgc attttttact
    19981 cttaacaaaa aataaggaac tgtgacaaaa tcgtgtcaca gtcccttatt tcgaataaac
    20041 aattttctag aagtagcttc ttcagaaatg atttattact tgatttctat tctttcgctt
    20101 ctgtcgctgt acctgtgatc aattcgattg cttttttcat tgtcacatca tgttccagca
    20161 tatcttctgt caacacgcgt ttgatttgtt cgattggcat gttgtatgtt tctgataatt
    20221 cttggatttc tgcattgatt tcttcttctg ttgcttgaag attttcagca gctgcaattg
    20281 cttcgatcac aagatttgtt cttgtgcgca tttctgcttc accttcgaat tgtttatgca
    20341 agtcttcttc tgtagaacca gttaattgat agtacatttc aggagagata ccttgacgtt
    20401 gcatgttgtt caagaattcg tccattgaac gatgtacttc atcatgaacc atcacgtgtg
    20461 gcaattcaac gatttctgcg ttttcaacag ccatacggat cgctgcttca tcttttgcat
    20521 cgtccgctgc tttttctttt gtttctacta attctttgcg atatttttct ttcaattcgt
    20581 ctaatgttga tacttcgtca tcaacatctt ttgcgaattc gtcatctaat tcaggcactt
    20641 cttttgcttt tacttcatgg attgtcactt tgaagactgc ttcttttcca gcaagatctt
    20701 ctgcttgata atcttctggg aaagttacat ttacatctag actttcacct gatttatgac
    20761 caactaattg atcttcaaaa cctggaatga atgagcctga acctaattca agtgagtaat
    20821 tttcgccttt tccgccatca aacgcttcgc catctacaaa gccttcgaag tcgattacta
    20881 ctgtatcgcc attttcagct gcagcatctt ctttgataac taattcagct tgtgcttctt
    20941 gttcacgttt gatacgtgca tccacgtcag catctgttac ttcacggtct tgtttttcaa
    21001 caactagatc tttatattgt cctaatttta cttctggttt cactgttact tcagcagtga
    21061 ttacccagtc ttgacctttt tccatgcttt caacatcgat ttttggttga gaaacaggat
    21121 cgatgccagc ttctttgaca gcagcttcat aagcttctgg taatacagca ttcaatgcat
    21181 cttcatataa agcttcttca ccgtacatac ggttgaatac ttgacgagaa actttccctt
    21241 tacggaatcc aggtacgttc aagctttttt tcactttatt aaacgcagtt gttaatcctt
    21301 tttggatttc gtcttgcgca atcgtaaatt ttaaaacacc atcattcgtg cctttttttt
    21361 caaatgttgc agtcatttaa ttccctccga gtttagaaat tcattttata catcaaaaac
    21421 gtttcatgca tacctttaaa tagtacacta accctttcaa attgtaaaca tattgaatgg
    21481 atagaatcct tgtttttcat aacattttta tctttttcac tgataaaatc taatggaaga
    21541 agatcatttg ctgcaaaatt ttttctcttt tttttaaaaa atcttgtttt tcatcttctg
    21601 taccttcata catcgttttt cctgtacacg cctctacagt cagttgaaac cagcgcttcg
    21661 ggtcttcgat gtatgtatca tagacaggat aaacaattgc agattgcaac gtgaattctt
    21721 cttttaacac ggaactaagc agcgcatctt cattcccgta acgatctgaa aatacacata
    21781 attgctggta acttgcctgc tgctgcggct ttggcagatc ttttggtatg actttgacta
    21841 acttttcttc aatcgtcaaa tagaaaactt gatggcattc gttcaattgc ctaagtgtct
    21901 ccagtaattt tgcccttgct aaaatatgga cctctggcga tacaataagt ttactcgctg
    21961 tttggataaa ctcatgataa ggaagctgtt cgatctcatc aatagacgca agctgacgtg
    22021 caggttccaa cgaaggaagt tcctccgatt tattgtggag tgccttgatc ttctgcctct
    22081 ccatttgtcc ggataattct tctgcaaatt ggattttttc aatgacccgc tgctcgaaag
    22141 cttcataaaa atctgtactt gctatcaatt ttctagcata taaaaaatct tgtgtactga
    22201 ttaacaagtc aaaataaaat tcagcaaact cctcattttg aagatactgt ttttcatgca
    22261 agagtgcttg cttcaatgct tcttttttct cgtccaactc gtaaagacaa aaaacaagca
    22321 ggctattagc ctcaaagctt tctttcaact cgtaagctcg ttgaaataat tcttttgcct
    22381 gctgatattg ctggtttgct agtgcttgtt gtgccactcg cattaaattc tcatattttt
    22441 ctggaaaatc aatcggcttc acgcagtgtc ctcctcttgc tcatattcag ctcatgttca
    22501 atagtagcaa aacaaaaaaa gcatgacaat taaaaataac tgaagcaccc tctaaaagct
    22561 agattttaga tctgactttt agaaggtgct ttcaactgtt actgcttttt attgctcatc
    22621 agctgttgac ttcctggttg aacgctttga tcagattgat cgtaacaaca cttgtattag
    22681 tgaaaaaatc gcccagatgt tgctttttct gattaaagac tgctggaaga taccctaaca
    22741 tcagcaaagg aaattttaga atgaaccggc aagcaccttc tctaactaaa acggttgacc
    22801 agctaagttc ttcttcatca aagctgatga ctcgaatacc aaagatcatt ttcccaatcg
    22861 tttggccatg attgaatttc gtcaataaaa taaaatacgc tagataaata agtaaggctg
    22921 tgaatccgta gagactcaga taagaatctg aaaaattaag ccaacctaga tttttggcaa
    22981 taccaagtgt taaacgcgta atagctccga tacacaaaag atcgaccaag aaggcaaaca
    23041 ttcggataaa aaaacctgca tagaaatact taggaaaatc atttgctttt ggcggttcat
    23101 ttcttgtgtc cgtatacttt ttccatttac gttgtttctt tttgatttct tcgggcgtca
    23161 aaggctctct ttcaaaagag ctcagtacct tttgggaaaa gttggagtca ggtcctacag
    23221 caggctgctg ttgcttatct ttttcttgtt ccttattttc atttgaaagc tctgacatgc
    23281 ttactcacct ccgtaataat acattgcttt tggtgattcc gctgtcccta gattctcaag
    23341 aacggataag atctgactag tctcagaagg cttcaatccc tggaattctg ctagtttact
    23401 tcctaaccat gtactagcaa acccagatgg cgtggaagaa tattctacta gttccgcatc
    23461 ttcaagctgt tgttcctttc gtatagcttt taaagcatct tcaggaaatc ccaattcatc
    23521 gactaaacct acttcttttg cttggacacc atcataaatc cttccatcag caattttttt
    23581 gacttcttct tcagatttat tccgtccctc gctgacgatt gctacaaaac gactatatgc
    23641 gctgtcgata tatgcttgaa gcaccgcttc ttcttctttt gtctctggtc gcgttcctga
    23701 acccatatct ttcaacgctc cgcttttaac tgttgtatct tccacaccca gtttttctag
    23761 aagtcctgaa taattcaatc ctgacatgat cacaccaatt gagccggtca ctgtttcttc
    23821 tgttgcaaag attttatctg cttgagcaga aatatagtag ccaccgctag ctgccatgtt
    23881 tttcatgctg acatacattg ggatatcatg ttctttacga atcgtatcca atttcttagc
    23941 gatttccgca ctttcgtaga caccgccacc aggagaattt acttctaaaa agataccttt
    24001 gactgttgga tcttcttcaa tcgccttcag ttgttccata aaatttttat gattgtaacc
    24061 ttcagaagca aataaacctg aagaccctcc cgcagcaatc gttccatcta ctgataattt
    24121 tacgattttt tgcgtactgt ctccctcttc caacacattt ggagctagtt cttcatcccc
    24181 atataaccaa ctgttcaatc cctcaatttg atcgttttcc tgaggcttag tcaaactagc
    24241 tgaaactaac gaaaccacaa ataatcctgc agcaatcaga actgctgccc aacgtctttt
    24301 attcattctt tctcctttct aatacaagtt ttttatgacg atcacagttt ctgactgctc
    24361 agaagtgtcg aaccgctctg ctttttcttc ccgcgcatac ccggaaaacg actcttttgt
    24421 taattcatct tctaccgtaa actcacgagc ttgagcaact tccaaggcat caggtgcgat
    24481 ttcttggatg ctatcccagc cagtatctaa aaatattttc gtatcatctg atacaccagc
    24541 tgatgcttcg aatttggaca attgtttttt taacattccc agtgtcatga tctcaatttt
    24601 tgaatccatt tttactcctc ctctatccga ttactttcta ttgtaccgaa ctcctgaaaa
    24661 agatgataga taaaaaccca ttattttttg acataaaaag gacatttgac aaagaatttg
    24721 atatatacca atttattttt tattcttttt ttgttgacaa gaacattcgc aaaaggttat
    24781 gattttaaat gtttccagat aaaaaacaaa aaggaggata gttatacctt ttaggatagc
    24841 gccaggtctg tgtaaacaga tgacgaggag agggtttatc gaaagattcg gcggatggcc
    24901 ctagggactg cactctacag aaaatccaca aaaaatgtct gcaaagataa aacaaaagga
    24961 ttttcctgca gctggaagca ttcatacgaa ttcattatag aaagtaggat gttttttatg
    25021 tgcggaattg ttggaatgat tggattaaaa aatgtaacac caggattgat tgacggtcta
    25081 gaaaaattag aataccgtgg ttatgattca gctggtatct ttgtctcaga tggcacaact
    25141 gattatctgg tcaaagcaca aggcagaatt caaaatttga aaaacaaaat aaatattgat
    25201 actacaggaa atataggtat tggtcataca cgatgggcaa ctcacgggca accttctgaa
    25261 gaaaacgccc atccacatac ttcccaaagt ggacgcttcg tgctcgttca caacggcgta
    25321 atcgaaaact ttgaagaact taaaattgct tatttagcaa atgaccactt tattggtgag
    25381 accgatactg agattatcgc tcatctaatt gaaactttcg cgaaggatac tacaacgcaa
    25441 gaggcgtttt taaaagcttt gcgggtcatc aaaggttctt acgctttcgc tttgattgat
    25501 cggacagcac ctgatgttat ttacgtcgct aaaaataaaa gtccactatt aatcggactc
    25561 ggcgatggat tcaatgtgat tgccagcgat gcaatggcta tgcttgctca tacgaaggaa
    25621 ttcgttgaga ttgaagatga agagatggtt accgtcacat cagaaaaagt tattattcaa
    25681 aattttgcag gtgacatcgt agaacgttct tcattcgaag cacaagtaga tgcttcagat
    25741 atcgagaaag gtacctatcc tttttatatg ctgaaagaaa tcgacgaaca gcctattatc
    25801 atgcgtcgta tcgctcaaaa atacgtaaca gaagacaatc gtgtctctct tgatcaaaag
    25861 ctggtcgata cattgtctga tagcgatcga atctatatcg ttgcttgcgg aactagttac
    25921 catgcggggt tagcaggtaa acagactttg gaaaaattga cacagattcc tgttgaggtt
    25981 catttagcca gtgaatttgg ctataacaca cctcttcttt caaaaaaacc attctttatt
    26041 ttcttgagtc aaagtggcga aacagcagac agccgccaag tgctggtaaa aatcaaccgt
    26101 ctcggctacc cttcacttac gattacaaat gttgctggtt ctactctttc gcgggaagca
    26161 tcctttacac tgctattgca tgctggaccc gaaatcgcag tagcatcaac gaaagcttat
    26221 actgcacaaa tagctgtact aactttatta gccaaagcta tcggtgataa aaaagaattt
    26281 gaaacttctc ttgtattcga tatcactcat gaattgagtt tagttgctaa tgcgatggaa
    26341 tcggtcatcg cccaaaaaga ttatttagaa gagttggcag aagtatatct cagcgataca
    26401 agaaatgcct tttacattgg acgtggtgct gactacaacg tttctcttga agctgctttg
    26461 aaattgaaag aaatcagtta tatccaggca gaaggttttg ccgctggcga attgaaacat
    26521 ggaacgattg ctttgatcga agaaggaaca ccagtgatcg gaatcatcag tgaagaagtg
    26581 actggtgcgc atactcgagg aaacctgaaa gaagtcgaaa gccgtggcgc taaaacacta
    26641 gttattgtct cagagggatt agaaaaggaa agtgaccagc tggtgctacc aactgtccac
    26701 ccttatctaa ctactctaac cacagtcgta ccgactcaat tacttgcata ttacgcaacg
    26761 ctattacggg gattcgacgt tgacaaacca cgtaatttag cgaaatcagt gacagtagaa
    26821 taataaaaga acaaggacca gtgtcagaca tctgacactg gtccttgttc ttttattttt
    26881 tatgaaaata tctcttaatc cagtccgatt tcttctttga ctactgccgc gattttatcg
    26941 acatagtaat taactttctc atcagtaggc gcttctgcca tcactcgaag taatggttct
    27001 gtaccggaag gacgaactag gattcttcct tctgatccca tttcagcttc tgcctcttca
    27061 atcacttttt tgattgccgg aacatccatc gcaccgtttt tatcacttac gcggatattc
    27121 actaattttt gcggatagat tgttacctct gctgcaagct ctgataattt cttacctgtc
    27181 tgtttcatga tattcagtaa ctgaattcct gaaagcatcc catctccagt tgtgttaaaa
    27241 tctaagaaaa tcatgtgccc agactgctcg ccgccaaagt tataatcgtt tttgcgcatt
    27301 tcttctacaa cgtaacgatc accaacttgt gtaacgacat ctttcaaacc gattccttcc
    27361 accgctttgt ggaatcctaa gttactcatg acagtcgtaa cgatcgtatc ttttttcaat
    27421 cgattttttt ctgccaagta tttagcacag ataaacatga ttttatcacc gtcgacaatg
    27481 tttccaagtt catccaccgc aataatacga tcgccatcgc catcgaaagc tagtccagca
    27541 tctgctcctt tttcaacgac catctcagct aaacgttctg gatgggtaga gcctacgccg
    27601 tcattgatat ttaacccgtc tggatttgtg cccattgtat aaaaatctgt ttcaagatcg
    27661 gcaaacaaac gattaacact tgttgaagtt gctccgtttg ctgcatcgac acaaatggtc
    27721 aaaccggaaa ggtcaccgtt gatcgtttgt acaaggaatt gagaatattt caataagcct
    27781 tctggaaatt cttctacagt tcccaatcct tcagctgatg gacgaggcaa ttcatccact
    27841 tcagcatcta ataatgcttc aatttctagc tcttggtcat ctactagttt aaaaccatct
    27901 gcaccgaaaa atttgattcc attatcttgt gctggattat gagaagcaga aatcatgacc
    27961 cctgcgcttg ctttttgcaa acgtgtcaga taagcaacac ctggtgtaga gatcacacct
    28021 agctggaaca cttcgatacc caccgataaa agtcctgaaa tcagcgcttc ttctaacatt
    28081 tgacctgaga ttcttgtatc ccgccctact aacacgcgtg ggcgttttcc ttcttcttca
    28141 tgctggctta ggacataacc tccgcaccgt cccagcttaa atgcaagttc tggcgtaagt
    28201 tccttgttgg cttcaccgcg tacgccgtct gttccaaaat atttacccat agttgtaaaa
    28261 ttactccttt aacttaattt tctgaggaat ttgttctgct catttgtgtt gattcgcttg
    28321 tttctgatgt ttcttctgta cttgtggtcg agctgcttgt cccagattcc gaactggaag
    28381 aaatggttgt tgtcgatggt tccgttcctg aatcagcaga tgaaatactt ctactcgtac
    28441 ttgtattttc ttgtgtccga ctgctcgtcg aagcagagct actgcttgac gtttgcaaca
    28501 cgggtgtaat ctccacacta actgattcgg gcttgatact taacccctct tctactggta
    28561 tttcaatttt acgagttgtt cgatgattga tacctgagac attgatcggt acagcaatac
    28621 tgtcaccgat cgatgcaagc aaatcgctag agccgcttac ttctgcttcc agttttgaaa
    28681 aactgaaatc atagctttct accccggcag ctggtgttcc ttgctgtatc ccataaagtc
    28741 gtaccgactt gcttggcttg atcgaatcag ctctcactgt tacttgcact ggatcagaga
    28801 cgatacttaa tacttcccct gctgcattca gcgcctgtat tgggacctca ctattaatcc
    28861 catcatctgt gaccatcgaa gcgtccacat cagctactac tcgtttgatt tctgccaatg
    28921 ttttgtcacc tgtagtgata gagacttttt taggactaac ggaagtatca tcaagttcat
    28981 atccagatgg tgtgaacgtt gaaggaatca acgtttccac atcgaaatct tttattacct
    29041 ttttctcaac cgttactgta atagtcgatg gttcgatctt tgctgtgaca gcactgctca
    29101 aattttgaat ttttagcgga acttcatgtg ttccttcgcc cagcttagtg atatctgctg
    29161 tgacgcggaa catccgtgta tcttcgtttg cctctgcatt caactgaact cggtttgcac
    29221 tggaaagttt gacagtcact gcattttcat atccatggat atagtagcgc tttgaatcat
    29281 aagaaatctt gatcggcacc cccgtaactg tttgattata cgcttcattt ccagaaagcg
    29341 tatttggaac ggactggcca tttgcattga agtacagcgc aatactgaac agcaatgcaa
    29401 taaaactgta aagaatgctt gtccgtttct cttttgagat catttgcgat cccctccctt
    29461 agtcataccg tctaggaaag attgaatcac gtttttcttg ttttttgttt tctcttcagc
    29521 aattagttct ctacgcaaga ttgccaaata ttcttgctgg gataagccag ccatcagttc
    29581 attatctaac gtgatgctga cgtccccggt ttcttcagag acaacgatgg taatcgcgtc
    29641 actgacttcg cttataccga cagctgcccg gtgtcgagtc ccgaattctt tagggatcaa
    29701 catactttct gatagaggaa gataggcgct tgctactgcg atctttcctt cttttacaat
    29761 aactgcaccg tcatgcagcg gtgtattagg aataaagata ttgatcagca gctccccagt
    29821 gatatctgca tctaaagcaa tccctgtctc aatatactca tctaatccag tatgacgttc
    29881 gatagtgatc aacgcaccga tttttcgctt ggacatatat tggatggctt tgtcaaaaga
    29941 caagatcatc ttctcatctt cctgctgttc gctttttgat tgtttgaaaa gcgaactgcg
    30001 tccaagatgt tccagtcctc tccggatttc cggctgaaaa atcacgatag cagctatcac
    30061 cccgtacgta atgacttggt tcatcagcca agacaaggtg tgcagaccaa tgatttccgc
    30121 tacgattcgg ataacaataa aaatagctac acctttcaga agctgaatcg cttttgtgcc
    30181 tcgcagcagt tggatcagtt tgtatacaag ataccagaca accagaatat caagtatgtt
    30241 caccaaaaaa ttcagagaga acaagtcacc ggaaaacatt tgctgccaat agtccaaatt
    30301 aaaaagttcc ccgatttgaa atgacataaa aagcctctct tctcttttga gatttcctct
    30361 ctagtatatc atatgtttta attttagagg ctgatatgct cgtatttttc ttgagaaaaa
    30421 cgcataattt tcaagaaaat tgcaagtatg cgttttattt ttctctgcaa taaatatttt
    30481 taaacttcag agaattattc attatatttt ttctgttatt tttccttttt ttcttttgta
    30541 gcttgttcca tttttttacg tcgttctgct ctggcagctc ttctagcagc tcttctttct
    30601 tctgcttcaa ggtccgtcgt tttttctttt ctagtcttaa cgggtacatc gatagattcc
    30661 tctggttcaa gtcgtttctt gattttttct aattctcccg tcagtgcagc atagttgtaa
    30721 aaacgtgttt tagcgtcgtg gacagtcttt tcaaataaag cttttgcaag atttggattt
    30781 gcgttatcta gcgaggaaaa gcgaacttgt ttcctcatga aatcaggcat agcagagaaa
    30841 tcaggttttt tgtagtccaa agtcattggg ttcttgtttt tttcgaccat tgtagggtta
    30901 tatcgatata atgaccaata accagaagaa acagcttctt ttgcttcttt aagcgattct
    30961 ttcattccgc caagcaagcc atgtgtgata caaggcgtat aggcaataat cagagaagga
    31021 ccaggatact tttctgcttc ttcaaatgcc ttgatcgttt gcatttgatt ggcacctgaa
    31081 gcaatttgcg ccacataaac atttccatat gtcatcgcca tcattcctag atctttcttt
    31141 gaagcatact ttccactagc cgaaaattta gcgattgccg aagctggtgt tgcttttgag
    31201 gtttgaccgc cagtattgga atagacttcg ttatccaaaa caaggatatt cacgtctgct
    31261 ccgctggcaa gtacatgatc gatcccgcca aaaccaatgt cgtaagccca tccgtctcca
    31321 ccaatcatcc attggctgtt ttttacgaat agatcctttc tcttgtatat atcctccagt
    31381 tcagggaaaa ttgatcgttc gctttcgatc gcagcttgca gttttgctgc tctttgctgc
    31441 gttccttcac tctcaaacat gtgtactaac cagtcttcca tcaattctcg tagttcttct
    31501 gatacttttg gcaatacttc tttgattgaa cttgccaaag cctcgcgacg agtctgtgcc
    31561 gctagaagca taccataacc aaattcagca ttatcttcca ataaagaatt tgaccatgca
    31621 ggcccttgac cacattcatt ggtcgtataa ggagaagcag gagaagcacc gccccaaata
    31681 gaagaacagc ctgtcgcatt cgcgatcatc attcgatcac cgaacatttg tgtcaataat
    31741 tttacataag gtgtttcgcc acagcctgag caagcgcccg agaattcaag aagcggttga
    31801 ttgaattgtg tagacaagac agtattcttt cctttgactg gatttgcttt ttgtcttaat
    31861 gtcattgcaa aagcccaatt cacagcttgt tctttctgtt cttcataagg cttcatcact
    31921 aaagcttttt cttttgcggg acaagcctct acacacagac cacaacctgt acagtcttcc
    31981 actgaaactt ggatacggta ttttacgccg tctgcgcctc gcatttctct cacgataaat
    32041 ccttctggcg cttcttccat ttcctcttca tctgctaaaa atggacggat ggcagcatga
    32101 ggacaaacaa aggcacattc attacacatc gtacagcgat cactaatcca ctcaggtact
    32161 tctaaagcga cgccccgctt ttccatagaa gccgttccta aaggcatttc gccagttgtc
    32221 attccatttg ctacaagtgt accaacagag agttgatttc cttcctgtgc attcactggt
    32281 tcaagtattt cttgtatata accagtgttc cttttattga ttttttcagg catctccaac
    32341 gttctccaat ggtctgggat cgcgacttga tgaagcagtt cggtcgtccg atcgattgct
    32401 tgtatgtttt tttctacgat agccattgat ttatgtccat agcttttcag ggcttcttct
    32461 tttagcagcg gaagcacctc atcaaaagga atgatgtcag ctaatttaaa aaaagccgtt
    32521 tccattgccg tattgatccg gcgtcccaaa ccgacttctt gggccaatct catggcattg
    32581 atcgtataga atctaatgtt attttcagcc aagtaacgtt ttaattttaa gggcagatgc
    32641 gtttctaatt gttcgtcgct ccaaagagta ttcaataaaa aaatgccacc tggcttcaat
    32701 cctttgacca agtcatagct atgcaggtaa gctggtgtgt gacaggcaac aaggtccgca
    32761 tgctcgacaa gatacgctga acgtatcggc gtatctccaa aacgaagatg agagacagtc
    32821 agaccgccag attttttcga atcataataa aaatagcctt gcgcatattt atcggtgtga
    32881 tctccaataa tcttgatagc tgatttattt gctccaactg ttccgtcaga tccaaatccc
    32941 caaaacttgg cctggaatgt ttgcggttct gtcaaatcca gactttcctt tggttccaaa
    33001 gattgataag tcacatcatc cacgatccct atggtaaacc gttttttgcg tatgcttggg
    33061 tcttttttca attcatcaaa aactgctgag atttggtcgg gagtcacatc tttagacccc
    33121 aatccgtagc gaccaccaat aacagccggg cgaagttcag aatcatataa cgcactttga
    33181 acgtctaata agagaggttc tccacctgcc cctggttctt tacttcgatc aagaacagca
    33241 attgttttta ctgtatgagg caacttttgc acgaatgttt ccactggaaa aggacgataa
    33301 agatggatat tcaaaaaacc tgttttccgt ccgtttgcat tcaagtaatc aatcgtttgt
    33361 tcaatcgtct gtgcaactga tcccatagaa acaatgactt cttccgcatc gtctgctccg
    33421 taataagtca ccaagtcata agaagttcct cgtaatcggt tgatctcttt catatatttt
    33481 tgaacgattt caggtaattt atcataatag gggttgatcg tttcccgttg ttgaaaatgg
    33541 atgtctggat tctggttcgt accactaaca gtcggatgat tgggattcat ccctcgttta
    33601 cgaaatgaag ctaatttctc ctgattgatc agaggagcta attcttcata ttccaaaact
    33661 tcgatttttt gaatctcgtg actcgtacgg aatccatcaa agaaattcaa aaaagggact
    33721 tctccttcaa tagaagcaag atgggcaaca ggtgctaaat ccatcacttc ttgtacactg
    33781 ctttcagcta acatagcaaa tcctgtttgt cttgcagcca tcacgtctcc gtgatctccg
    33841 aaaatattca acgcatttgt cgtcactgcc cgactagcaa cgtggaaaac acttggcagt
    33901 agttccccag caatcttgta catattcggt atcatcaaaa gcagcccttg tgaagcggta
    33961 tatgtagttg tcagtgcacc agttttcaat gatccatgga ccacaccagc tgctcctgct
    34021 tcggattgca tttctactac ttttactggt tgaccgaata cgttttcttt tcctgctgct
    34081 gaccattgat ctactaattc agccatggtc gaactgggtg ttattggata tattgcagca
    34141 atctcggtaa atgcataaga aatatatgcg gctgccgtat tgccatccat tgttttcatt
    34201 tttcgcatga ttcatcatcc ttgttcatat tctctcttat tatctgttcc attataacgt
    34261 aaaacatgcc atttttttct tttatttata aaaatatttt acatgtatct atttgaattg
    34321 gtcatctaat ttttagacaa acagtctaaa tcgttgcctt tttttgtcag acatgtataa
    34381 actataagcg aaagaggtgt tttaacatgg atatatcagt gattgatgca acgaaaacaa
    34441 atacacaaaa aggaatcctt tatggttcat cgaatgctcc taaaaaaatg gtcgaattca
    34501 tcaacttagc ttgtccgtat tgccgacaat ggtttgaaga atcttatgag ctattagaag
    34561 aagcagtcca aagcgggcaa ttgcagcgag tgatcaaatt gttcgataaa gaaaaagaaa
    34621 gcttacagcg agggaacgtg atgcaccggt atctcacaat cagcgatggc caaaaagcga
    34681 tcaaagagat caaacaaata ttcgatactc aagacgagtg gaaacatctt tctctccaag
    34741 aagtagcgga ttttgcagta gataaactaa agctgacaga acaaaaagac gaacagctga
    34801 gccaagctgt catcaacgaa gctgagcaag cacatattcg atttgttcca actgtcatct
    34861 tgggtaaaga aatctttgat gaatctattt caatagagga attaaaagaa ttgatccaaa
    34921 caaaataaaa aaataaggag cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta
    34981 aacacgtata aacggcggaa gcagaagcaa ctc
//
LOCUS       DAJSKS010000011        32588 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJSKS010000011 DAJSKS010000000
VERSION     DAJSKS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.011"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(42..230)
                     /locus_tag="ON396_000515"
     CDS             complement(42..230)
                     /locus_tag="ON396_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR3112648.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            500..862
                     /locus_tag="ON396_000516"
     CDS             500..862
                     /locus_tag="ON396_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3112649.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            914..2596
                     /locus_tag="ON396_000517"
     CDS             914..2596
                     /locus_tag="ON396_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HCR3112650.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2713..4749
                     /gene="recG"
                     /locus_tag="ON396_000518"
     CDS             2713..4749
                     /gene="recG"
                     /locus_tag="ON396_000518"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR3112651.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4798..5799
                     /gene="plsX"
                     /locus_tag="ON396_000519"
     CDS             4798..5799
                     /gene="plsX"
                     /locus_tag="ON396_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR3112652.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5921..6163
                     /gene="acpP"
                     /locus_tag="ON396_000520"
     CDS             5921..6163
                     /gene="acpP"
                     /locus_tag="ON396_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR3112653.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6431..7435
                     /locus_tag="ON396_000521"
     CDS             6431..7435
                     /locus_tag="ON396_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3112654.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7436..8380
                     /locus_tag="ON396_000522"
     CDS             7436..8380
                     /locus_tag="ON396_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3112655.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8380..9342
                     /locus_tag="ON396_000523"
     CDS             8380..9342
                     /locus_tag="ON396_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3112656.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9369..10283
                     /locus_tag="ON396_000524"
     CDS             9369..10283
                     /locus_tag="ON396_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3112657.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10309..12090
                     /locus_tag="ON396_000525"
     CDS             10309..12090
                     /locus_tag="ON396_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3112658.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12438..13124
                     /locus_tag="ON396_000526"
     CDS             12438..13124
                     /locus_tag="ON396_000526"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR3112659.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13144..16725
                     /gene="smc"
                     /locus_tag="ON396_000527"
     CDS             13144..16725
                     /gene="smc"
                     /locus_tag="ON396_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCR3112660.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16734..17543
                     /locus_tag="ON396_000528"
     CDS             16734..17543
                     /locus_tag="ON396_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3112661.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17554..18552
                     /gene="ftsY"
                     /locus_tag="ON396_000529"
     CDS             17554..18552
                     /gene="ftsY"
                     /locus_tag="ON396_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR3112662.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18739..21006
                     /gene="gshAB"
                     /locus_tag="ON396_000530"
     CDS             18739..21006
                     /gene="gshAB"
                     /locus_tag="ON396_000530"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HCR3112663.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21095..21547)
                     /locus_tag="ON396_000531"
     CDS             complement(21095..21547)
                     /locus_tag="ON396_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HCR3112664.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21697..22647
                     /locus_tag="ON396_000532"
     CDS             21697..22647
                     /locus_tag="ON396_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCR3112665.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22640..23599
                     /locus_tag="ON396_000533"
     CDS             22640..23599
                     /locus_tag="ON396_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCR3112666.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23596..24351
                     /locus_tag="ON396_000534"
     CDS             23596..24351
                     /locus_tag="ON396_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3112667.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24374..25327
                     /locus_tag="ON396_000535"
     CDS             24374..25327
                     /locus_tag="ON396_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3112668.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25971..26267)
                     /locus_tag="ON396_000536"
     CDS             complement(25971..26267)
                     /locus_tag="ON396_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HCR3112669.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26622..26978
                     /locus_tag="ON396_000537"
     CDS             26622..26978
                     /locus_tag="ON396_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR3112670.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26988..27887
                     /locus_tag="ON396_000538"
     CDS             26988..27887
                     /locus_tag="ON396_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCR3112671.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28404..29135
                     /locus_tag="ON396_000539"
     CDS             28404..29135
                     /locus_tag="ON396_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR3112672.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29280..31307
                     /locus_tag="ON396_000540"
     CDS             29280..31307
                     /locus_tag="ON396_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCR3112673.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            31415..32512
                     /locus_tag="ON396_000541"
     CDS             31415..32512
                     /locus_tag="ON396_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCR3112674.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT        10874 a         5672 c         7005 g         9037 t
ORIGIN      
        1 aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt
       61 cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg
      121 cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg
      181 ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc
      241 tcctttgcct tgatttggag caatagaata attcagctca taccagacta acatatcata
      301 tagaatggaa atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt
      361 gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg
      421 ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac
      481 ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat
      541 taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt
      601 aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa
      661 ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac
      721 gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa
      781 atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg
      841 tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag
      901 gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca
      961 ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc
     1021 tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc
     1081 agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt
     1141 gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc
     1201 taagcaaatt ccagatgttg tctcattaaa tgcttccgat cttgctgcag ccttcactca
     1261 tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt
     1321 agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga
     1381 agtaatgaca gctgttgtaa aaggggcaaa gcgtgcttta gctaaaactc cagacttgct
     1441 tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta
     1501 tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc
     1561 tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat
     1621 ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg
     1681 acctacagtc gatagtacat tcgattacga agaattccgt aactatttag atggtatagg
     1741 agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa
     1801 cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga
     1861 caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc
     1921 gaaaaaagaa acaccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt
     1981 ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtgggc aaacaatgaa
     2041 tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt
     2101 cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat
     2161 cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt
     2221 caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt
     2281 tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa
     2341 agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac
     2401 agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat
     2461 cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga
     2521 aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct
     2581 attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa
     2641 aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaagggagg
     2701 gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa
     2761 aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat
     2821 tatcctttcc gctacgaaga tattcaggaa agaaatctaa acgagatcca agatcaagaa
     2881 aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa
     2941 aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt
     3001 aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa
     3061 tgggatgcaa agcgtaaagc attgaacggg atgaagattt taggttctca aacaatggat
     3121 gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta
     3181 atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt
     3241 gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa
     3301 gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt
     3361 caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc
     3421 ctttatgatg tccaaaaatt gaaagaattt actcaaaagc taccttttga actgacaaat
     3481 gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag
     3541 cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac
     3601 gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa
     3661 caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgctt
     3721 acggggtcaa cgaaagcaaa agaacgtcgt gagctccttg aaaaactaga gcaaggggaa
     3781 attaacatcg tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta
     3841 ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt
     3901 gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta
     3961 gctattacag catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt
     4021 atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg
     4081 aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaagaatca
     4141 gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca
     4201 ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc
     4261 atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta
     4321 ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg
     4381 gcacagctgc atcaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc
     4441 ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc
     4501 accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt
     4561 ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat
     4621 gttttggagg tagccagaga agaggctgca gccctttggc agataaagga ttggcagatg
     4681 cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat
     4741 ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg
     4801 aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg
     4861 gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa
     4921 atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc
     4981 aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt
     5041 gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc
     5101 gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc
     5161 ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt
     5221 ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt
     5281 tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca
     5341 gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca
     5401 agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta
     5461 gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg
     5521 aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc
     5581 gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat
     5641 ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc
     5701 cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt
     5761 ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcaat
     5821 ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg
     5881 taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt
     5941 taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga
     6001 tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt
     6061 agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac
     6121 agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa
     6181 gaaggataaa aagctgcata cagcttttta tccttctttt tttaatcttt ttgacacgta
     6241 aaagtcattt ttctgataat tctggcgata aacagaaatt atgttgcaaa gtaaagcgat
     6301 tttctatata attattaata attcattaca caaagataag aataaccttt gttataaaac
     6361 gctttattct gtgtaatgtc cttttttatt aagaaaaact tccatttagg aagcagagag
     6421 gggtattcga ttgtccgata accaattatt agaagttcag aacctacata caggattccg
     6481 actcaaagat gactattacg atgcagtaga tgacgtttca tttactttag acaaaaacga
     6541 gattttagca atcgttgggg aatctgggtg cgggaaaagt accttggcta ctacgattat
     6601 gggattactt gatcctaata acacaaaaat tacaggtgag attttatata acgatctgaa
     6661 tttaatcgac ctgaacgaaa cgctctataa taaaatccgc ggaaatgata tcggaatgat
     6721 tttccaagat ccgttgtctg cattaaatcc tctcatgaga atcgaagacc agatcaaaga
     6781 aggactttcc tatcacacaa agatgaatgc agaacagcgt caagctcgtg cactagaatt
     6841 gctagaacaa gtagggattc ctaatcctgc gagagttgga cgccaatatc cccatgaact
     6901 atcaggcggt atgagacaac gtgtcatcat cgcaatcgca atcgcctgca aacctccaat
     6961 cgtcattgca gatgaaccga cgacagccct agatgtaacc atccaagcac agattcttga
     7021 tttactgaaa gaccttcagg aagaaacaaa aacagggatc atcttgatca ctcatgactt
     7081 aggagtcgta gcagaaatgg ctgatcgagt ggcagtgatg tatggcggac agttcgttga
     7141 agtagcaggt gtaaaagaac tgtttgatca tcctaagcat ccttatacac gctctttact
     7201 gaactctata ccacaagaag gaaatcatga tgcagaactc catgtgatcg aaggtgtcgt
     7261 tccttctctg aaaaacatgc ctcgtaaagg ttgtcggttt gcaccacgta ttccatggat
     7321 accagcaagc gatcatgaag aagagcctat ccttcatgaa gtcgcaccga accacttggt
     7381 acgttgctcc tgctataagc atttccactt tagagacgaa aaaggagagg tgtagatgac
     7441 agaattaatc acaatcaaag acctgaaagt tcattaccct attcgcagtg gattcttcaa
     7501 tcgagtaacg gatcatgtat atgctgtaga tggtgtggat tttattatcg aaaaaggaaa
     7561 gacatatggt ttagtgggag agtctggctc tggaaaatct actacgggaa aagccgttgt
     7621 gggattagaa aaggtaacag ccggacaaat catgtatgaa ggaaaagatg taacgaagcg
     7681 aagcaaccgc aaaaaaatgg gttataacaa ggatgtgcag atgatcttcc aagattcaat
     7741 gtccagtttg aatcctaaaa agcgagtgtt ggatatcatt gctgaaccca tccgaaattt
     7801 tgaacggtta agcgatcaag aagaaaagaa aaaggtaaaa ggactgctag atatcgttgg
     7861 gatgccggaa gatgcgctat ataaatatcc tcatgaattt tcaggcggac aacgtcaacg
     7921 tctaggagta gcccgtgctg ttgctaccaa tccaaaactg atcgtagccg atgaacctgt
     7981 atctgctctg gatctatctg ttcaagcgca agtattgaat ttcatgaaac gaatccagca
     8041 agaatacggc ctcagctatt tattcatttc acatgattta ggtgtagtaa aacacatgtg
     8101 cgacaatatc gcaattatgt acaaaggtcg atttgtagaa atcggtacaa gagaagacat
     8161 ttataatgac ccgcgacata tctatacgaa acgtctgctt tcagctattc cgcaaatcga
     8221 tgtagaaaat agggaaagcc acaaagaaaa tcgacgaaga gtagaacaag agtatatcca
     8281 aaaccaaaaa gaatactatg atgcgactgg tcgagtctac gatttgcgta cgatcacaac
     8341 tacacacaaa gtggcgttga aagatggagg tgcaagctaa tgtggaaaac gattctgcga
     8401 cgtgttttat tgatgatccc gcaagttatt attttaagtg ttctgatctt cttattagcc
     8461 caagcgatgc cgggggatcc atttaccggt ttgatcaatc caaatcagga tccggcagtg
     8521 atcgaacaga tgcgacaagc agctggcttg aatgacccat ggtatgtcca gtacttccgt
     8581 tggatcggaa atgctttgca aggcgatttt ggtcaaagtt tcttatacaa acgctctgtt
     8641 gctgacttga tcggcgaacg gatcatcaac actttatatc tatcgatctt gacagttgtc
     8701 cttacttatt tgattgctgt gcctctgggg atgcttgctg gccggtatca aaattcaatc
     8761 ttggataaga ttgtcgtaat ctacaacttc tttagttttg ctgttccttt atttatcttc
     8821 ggcttgatca tgctatttgt ttttggttat cgtttaggct ggttcccaac aagtggatca
     8881 caaacactag gtttgcaagg tggatttatc actcaatgga tagaccgttt gaaatatatt
     8941 ctgttaccat caattaccca agcatttttg gcgactgccg tcacaatcca atatctgcga
     9001 agtgaagtaa tcgattcgaa atcgctcgat tttgtcagaa cagcccgttc caaaggggta
     9061 ccgacgaata aagtattcag tcgacatatt ttccgaaatg cagcgttgcc gatggcttct
     9121 caaatggggt atgaaatcac ttcgttgatt gctggttctg tagttatcga aaagattttt
     9181 ggttatccgg gagtcggaaa actatttatt gattccatcg gccaacggga ctataccgtg
     9241 atcacatcct tagtattgat tttaggaatc gcgacgctag tggggacatt actttcagat
     9301 atcatcatga gtatcgttga cccgcgtatt cggattcaat aaaaccaatc agaaaggaga
     9361 atggatcgat gagtgaaaaa aacaatacag cagttcaaga aagtattcct cctatgggga
     9421 tacgaatgat tgctcgagaa tttaaaaaag ataaaattgc tattttttca ttaggtttgc
     9481 tagtggtttt attattagtg atctttatcg gtgcgatgct gaccgatcaa gacaaagtga
     9541 tgtatgttag tatcttcgac aaatacgctg caccaggaac agtttcgaca caaggacaaa
     9601 agttcattct gggagcagat gaaggcggac gtgatgtatt aggacaattg atcataggtg
     9661 ctagaaactc tgttacgatc ggatttgcga tcacgatcct tacttcgatc atcggggttg
     9721 gattagggat catctctggc tactacggag ggatcattga caacatcttg atgcggatcg
     9781 ttgattttat catgattcta ccgatcatgc tgatcattat cgtcttcgta tcgattattt
     9841 ccagatacaa tgtctggtcc tttatattca tcatgagtgc attttattgg gtagctaaag
     9901 cccgattatt ccgaagcaag acattatctg aagcaagaag agattatgtc agtgcctcaa
     9961 aaacaatggg aaccaatgat tttaaaatca tgttccgcga aatcatgccg aatctaagtt
    10021 cattaatcat tactaatttg acgatgaact ttgcggcaaa catcggtatc gagacaactt
    10081 tgactttctt aggtttcggc ttgccggcaa atgtacctag tttaggtaca ttgatcggtt
    10141 atgcaagtaa cggagaagtc ttagtgaatc ggcaatggat ctggttaccc gcatcaatcc
    10201 ttattttagt tttgatgttg agtatcaatt atgttggaca agcattcaag cgttctgcag
    10261 atgcgcgcca acggttaggc tagtatagaa gcgaaggggg aaataggaat gaagaaaacg
    10321 atttttggat ttgcagcgct gatgtctgct gtcgttcttg cagcatgcgg gggcggaggt
    10381 acagatacct ctggaaacaa ctctggaggc aacggcaata acaaagagga attaactttt
    10441 ccaattgcaa caaaaaataa tgatgatgca atcgaagacg gcgaattaga agctgcggtc
    10501 gcaacggaca gtcagtttaa aggattgttc cagtgggagt tttaccaaga ttcatatgat
    10561 gcagctttta tggctccttc tcatgaaccg ctatttgcaa acgatgacga tttcagaatc
    10621 acagacgacg gagctgcaac gcttgatcta gatgaagata cgaaaaaagc gacaatcaaa
    10681 gtaaaagaca acgtcaaatg gtcagatggt gaagatgtaa cggcggacga tgtgattttc
    10741 ccttacgaaa tcattgggaa taaagattac actggtatcc gttatgacga cactttccgt
    10801 aacatcgtag gtatggaaga atatcatgat ggaagtgccg acacaatctc cggaatcaaa
    10861 aaaatagacg acaaaacagt tgaaatcgaa tataaagaag taaacccaag catgcttcaa
    10921 tcgggtggtg gtatctggtc atatgcagca ccaaaacata cgttagaagg tgtagcaatc
    10981 aaagatatgg aatcaagcga tcaagtacgt aaaaatccag tgacttttgg cccttattac
    11041 atgagtaata tcgttgcagg agaatcagta gaatacttgc caaacgagca ctactggaat
    11101 ggtacgccta aattgaaaaa gatcacgatg aaatctgtac cgatggcaag tgcaactgaa
    11161 gcattgaact ctaaattata tgatatgatt ttccaaatgc caacagatac ttacgatacc
    11221 tataaagaaa ttgctggcta tacaaactta ggccgtgaac aaacttctta tacttatcta
    11281 ggattcaagt taggtaaatg gaatgcagat acaaagagtg tggaatacaa tccagattca
    11341 aaaatggctg ataaatcatt acgtcaagca atgggctatg cattagataa taatgcagta
    11401 ggtgaacgtt tctatgcagg tttgagaagc aatgcgactt cattgattcc tccagtattc
    11461 cagtcatacc atgatgatga gatgaaagga ttcacacaag atctagacaa agcgaaaaaa
    11521 ctattagatg atgctggcta caaagatgtg gatgacgatg gtttaagaga agacaaagat
    11581 ggaaataaac taacaatcaa ctttgcttcg atgtccggtg gggaaacagc gcaaccattg
    11641 gcagattact atatccaaca atggaaagaa attggtttgg atgtccaata cacaacaggt
    11701 cgtctgatcg aattcaattc tttctatgat aaattagaaa acgatgatcc tgaaattgat
    11761 gtttaccaag ctgcttggaa tacaggaact gatccaaatc caagtggact atggggacca
    11821 aatgctgcgt tcaactacac tcgttatgaa tcagaagaaa atacaaaatt gatcaacgat
    11881 atcagttcaa gcaaagcatt tgatgaagag taccaaaaag aagcatatca aaaatggcaa
    11941 caatatgcat ttgaagaagc atttgcgatt ccaacgttgt tccgtaatga agtattgcca
    12001 gtcaacgacc gtgtcaaaga ctgggattgg tcttatgatg taccaaaccc atgggcaaat
    12061 gtttctgtta cagcagattc acgttcataa tcactaaaaa tgaccgaaaa agggcgatgt
    12121 cctttttcgg ttttttgata tttttctgtt tatttctcta atattcaggt caaggcgctg
    12181 atttcatgac ttctccaata aacggtgttc aaagtccagt ttctcggtat taagtcaaat
    12241 ttatgcaaaa atatggaaag ccattttcac aaaatttttt cttcatactc gaaactaaac
    12301 gaatttttca caacttctcg tcaatcacta ggattattaa agctttactt accttttgac
    12361 cgaaccattt caaatttgac aaaaaaccag tataataagg aagtatcgaa aaaatatcca
    12421 taaaaggaat gtggaaaatg gacaaccagc tgacaaaaga actaaaagaa aactataaca
    12481 tcgtttttca cgatctaaat ttgcttgaac aagcttttac ccattcatcc tatgtgaatg
    12541 agcatcgcaa tctgcaatta tcggataatg agcgacttga atttttagga gatgccgttt
    12601 tagaactgat ggtttctcag tatttattcc gtttatatcc tcatttgcca gaaggaaaat
    12661 tgacaaaaac acgagctgcg atcgttcgcg aagacagctt gtcgaaattt gccaaagaat
    12721 gtcactttga tcaatatgtt ttgttgggca agggagaaga gaattcagga ggacgtacgc
    12781 gaccagcatt gctttgcgac ctattcgaag cttttttagg cgcgttatat cttgaccagg
    12841 gattcgacac gacccatgca ttcatcgaaa aagtgatttt tcctaaagtc aaagcaggtg
    12901 ctttttcaca tgagatggat cataaaacaa aactacaaga agtcttgcaa aaatcaggtg
    12961 atgtatcaat cgaataccga ttgatcaatg aagaaggtcc agctcatgaa cgagtatttt
    13021 ggatcgaagt ttatgtagat gatcaattga ttggcactgg acaagggaaa tcgaaaaaat
    13081 tagcagaaca agctgcggcg gaaaatgcat tggcagcact gtaaaagtaa gtgagggaaa
    13141 gctttgtatt tgaaacgaat tgaaatagcc ggttttaaat catttgcaga caagacagtc
    13201 attgattttg aaaatagcgt cacagcagtg gttggaccaa atggcagcgg aaaaagcaat
    13261 attacagagg ctatccgctg ggtattagga gaacaatctg ctaaaagtct gcgaggcgga
    13321 aaaatgccgg atatcatctt tgccggttcc gatacccgta agcagctgaa tattgcagaa
    13381 gtcacagtga ttttagacaa tagtgatcat tatctgccat tggagtacaa tgagatcagt
    13441 gtaacccgcc gttatcgacg gactggggaa agcgagtttt tcatcaataa acaaagctgc
    13501 agattaaaag atatccaaga attgttctta gattctggat taggaaaaga atcgttctca
    13561 atcatttctc aaggaaaagt agaagcgatt ttcagtagta aaccggaaga ccgtcgtggg
    13621 atctttgaag aagcagctgg tgtcttgaaa tacaagcagc ggaaaaagaa agcagaacag
    13681 aagctttttg aaacagaaga caacctcagc cgcgtccaag acatcatcca cgaattggaa
    13741 gaacagttaa ctcctttggc cgcccagagt gaggcagcaa aggagttttt acggctaaaa
    13801 gaaacactga ctcaaaccga tgtctctttg atggttgctg aaatcaagac agcaaaaaaa
    13861 gattgggaca acaaacaagc acagcttgcg aaattcaatc tagaattagg caaactatct
    13921 gagtcgatcc aagaacaaga atccattttg gcgaagcagc gtaaagaaaa tgcgcaagcc
    13981 gatcgtctga tcgaaaaaaa tcagcaagta ttattagatc tttcggaaaa actgaagcag
    14041 acagaaggac aaaaagatgt tttgcaggag cggacaaagc atacacaaaa aagctcgcaa
    14101 gaatatcaaa cttcattagc agaagcacaa aaaaaggtta aacattttga aaagcttcaa
    14161 gaaagcttga tgaaagcagc agcagaaaaa gaaacagaga tccaaaaagc tgaagcaaat
    14221 ctcataaaaa cacagcaaga attagaaaaa taccaaaaat caacgaaaga attattggca
    14281 gaattaagag atcaatatgt tgatttgatg caagaacaag cagctgtagg aaacgaactg
    14341 aagtatctag aacgtcaata tatacaagaa acagcaaaaa gcaagcagac gttagccaag
    14401 caatcggaag tagaagcatc agtagaccga ttgatcttac aaaaagaaga attgacacaa
    14461 aagcaggcgc agctaaaatc atctttaact gaaacaaaag agaagttaga aatgatccaa
    14521 caaaacggga aaaagttcca agaagcatta gcaaaagaac agcctaaaat gtatcaatta
    14581 atgaatcaag tccagcagct acgtgctcgt aagaaaagcc ttcaagaaat ccaagaaaac
    14641 tattttggtt tttaccaagg ggttcgattg gtcttgcagc ataaacagca gctttcgggt
    14701 attgtcggag cagtggctga actgatcgat gttcctgcag atttcacgtt agctatcgaa
    14761 gcagctttag gtggggcagc ccagcatgtc atcgttgaaa atgaaaaaga tgctcggcaa
    14821 gccatcacgt atttgaaaca gcaaagagga ggaagagcaa cattccttcc actgacgaca
    14881 atcaaaccaa gacaattacc ggctcatata ttgacacaag cagcagctgt agaagggttt
    14941 atagggattg ccagtgaaca agtcagttat cccgatcaaa tccaaactgt ggttcaaaac
    15001 ctgctaggaa cgatcctttt agccaaagac ttaactagtg ccaatgcaat cgcgcaaacg
    15061 atccgctatc aatatcgagt ggtttcctta gaaggtgatg tgatgaatgc cggaggatcc
    15121 atgacaggtg gagcgaataa acgaggaaac caaggcagtt tgtttgtaca aaatcaagaa
    15181 ttgaaacagt tgactagtga atttgaagaa gcagataaac aactccaagc acaagaaaaa
    15241 aaagttcagg agctgcaaca agaaacagct cgtttagccg aagaacaaga agttttgcgt
    15301 acacgaggcg aacaattacg ttttgaagaa caagaagcga caaatcagct acaaaacatc
    15361 ataaatgaac ttgaacgttt tgaaaaagaa aagcaaatct caacttttga gacacgtgaa
    15421 ctgcagcagt ttatcgaaga ttacgaaaag caaacgaacg aattgaaaga caaacaaaca
    15481 gatcttgaat cgcaacgaca aaaaatcgac gaagaaatca aatcgttaag tcaagaaagc
    15541 gaccagatgg aagccagaag agcacaagta caaagtcaaa aagcacaaga acaagcagat
    15601 cttgctgtct tgaaagagca gtttaatcat ctgcagatcc agctccgcgg tgcccgagtc
    15661 caaaaagcag aggcaaccga acgtcaagaa gcgatagaaa agcagttggc gacattgaca
    15721 gcagactttt ctgatcatga agtcacagaa gaaagtttgg aaaaacaaat taacgaatta
    15781 tctgctcagc gagaaacatt aaaagccgag ttggcaaaag ccaaagagca gcgagataga
    15841 aggcaaaagg agatcgataa gcttgaagca gtcttggcag agcgtaatcg tgagcaaaaa
    15901 gcacgactgt cggaacaatc caaattagaa gtccaaaaag accgagctga aatgatttta
    15961 gataatcatt tgacctatct gcagtcagaa tatcagctga catttgagaa agcttcccaa
    16021 gactatcaag aaacaaccga tatcgaagac agtagaacaa aggtttcatc cttaaaagaa
    16081 cagattgaaa aactgggacc agtaaactta aattcaatcg aacaatacga acaagtcagt
    16141 gaaagacata cattcttagc aacacagcga gatgatctgt tagcagcgaa aaaccaattg
    16201 tttgaaacga tggatgagat ggatgatgaa gtgcggacaa gattcaaaga agtatttgaa
    16261 gctatccgtc aagaattcaa aattgtcttt ccaaatatgt ttggcggcgg taaagctgaa
    16321 ttagtactga cggaaccaac tgatttgctg aaaacaggta ttgagattga agtacaacct
    16381 cctggcaaaa aactgcaaag tctaagtttg ctttctggag gagaacgagc acttacagcc
    16441 atcgcgttgc tcttttccat tatccgtgtc tgtcctgttc cgttttgtat cttagatgag
    16501 gtagaagcag cgttagatga agccaacgtg aaacgttttg gtcgttactt gagcgatttc
    16561 caagatgaca cacaatttat cgtagtcaca caccgcaaag gaacgatggt cgcagcagat
    16621 gtactgtacg gagtgacgat gcaggaatcc ggtgtatcaa aaatcgtttc tgtacggatg
    16681 gaagatatca atgaagaggc gaaaatcaac gaaacgaaag tgtaggaacg aaaatgatca
    16741 aattagtagc aatcgatttg gacggtacat tattaaatga acaaaagctg atttcagatg
    16801 agaataaaca agcattagca caagcaaaac agcaaggggt caagatcgtt ctatgtacag
    16861 gacgcccctt agctgcgatg gcgcattatc tgcaagaatt aggactggta gatgaaggtg
    16921 actacagcat cactttcaac ggcggtttgg tacaaaaaaa tgatactggt gaaatcatgg
    16981 aaaagaaagt gatggaagta tcagatatcc aacgcttata caaactggct cagcaattgg
    17041 atctgccact agatgttttg tctgacaatg tggttttaca gctgcctagt gccccaagga
    17101 agcaatcctt gtacaatgtg ttgaataagc tgctgcaatt ccaaccagcg gatataagcg
    17161 atattacaga tgaatttgtg cttaataagg cagtaatcgc ttatgaccaa gaagaattag
    17221 atcctaaaat caaagaaata ccagcagaat tccatgaaat gtatgagatc atcaaaacaa
    17281 gaagtatgct tttggaattt atgccaaaag gtgttacaaa agcttatggt atttctttat
    17341 tggcaaaaga tcttggtttg aaacaagaag aaatcatggc gatcggtgat gaggaaaatg
    17401 atttaccgat gatccaatat gctggactag gcgtagcgat ggagaatgct gtgcctttcg
    17461 tcaaagaagc agcagatttc gtgacagcca gcaatgaaga aaatggcgta gccaaagtga
    17521 tccaaaaatt tgtattagag tagggggagt caaatgggat tttttgataa aatcaagcgt
    17581 gcttttactg gcgaagagaa aacagaagaa gttgaagtac aggagaagta cgacaagggt
    17641 ttagaaaaga cgcgtaaaac atttggtgaa cggatgaatg agttgtttgc caattttcga
    17701 acagtcgatg aagacttttt tgaagaatta gaagaaacac ttatcggtgc agatgtaggt
    17761 tttgaaacag cgatacgaat caccgaagca ttacgtcaag aagtgaaact cagaaatgcg
    17821 aaaaaatcgg cagaagtcca aaatgcgatc atcgagaaac ttgtggatct ctatgaagcg
    17881 gaaggaatca atgaagtaaa tgaactaaat atccaagaag acggactaac agttattttg
    17941 tttgtcggtg ttaatggtgt agggaaaaca acaagtattg gtaaacttgc ccatcagttc
    18001 aaaaacgaag gtaaaaaagt attgatggca gcagcagata ccttccgcgc tggagcaatc
    18061 gaccagttag ttgtctgggg cgaacgcgct ggtgttgaag ttgttcgtgg caatgcagga
    18121 ggcgatccag cagctgtcgt attcgatgct ttggaaagag caaagacaga gcaggcagat
    18181 gttttactag ttgatactgc aggccgactg caaaataaag tgaacttgat gaacgaatta
    18241 gaaaaaataa aacgagtaat ccagcgggaa ttaccagaag caccccaaga aacacttctt
    18301 gtattagatg caaccactgg tcaaaacgcg atggttcaag caaaacaatt caaagaaaca
    18361 acagatgtga ccggtcttgt tttgacaaaa ttagatggta cagcaaaggg cggtatcgta
    18421 cttgctatcc gtaatgaatt gcatttacca gtgaaattgg tcggtcttgg tgaaggaatc
    18481 gatgacttgg agccgtttga tccaaatgat tttgtcgttg gattgttcaa aggattacta
    18541 aaagaagaat aacccatgtc taaaaagcag gagctgtgac aaaagttgca gctccttgtt
    18601 tgaatgattg tttttaacat agctgtgttt ttgatgcctt catttttata cttatttgta
    18661 agcggttctc aaacaatttc gttctttttc ttagactttt tgattataat tagattcata
    18721 tgaattggag gtcacttaat gatgaatttt aagcaattat tattgcatgt caatgcgcgc
    18781 ccttttatcg accaagctcg atttggtata gaacgggaag gacagcgggt tgatcttgca
    18841 ggaaatctag caaaaaccga tcatccagca atctttggcg atcgatccta tcacccctat
    18901 atccaaacag attttagcga aacacaaaca gagatgatca cccctgttac cgattctatt
    18961 cccgaattat ttcagtatct cgctgctgtt tatgatgtga ctgctcgttc tataccgaaa
    19021 gaagagatga tctggccatt aagtatgcca cctgccttac cggaaaaaga cgaagagatc
    19081 attattgcaa aattaaaaaa tttcgaagat gtcttgtatc gacgttattt agcaaaaaaa
    19141 tacgggaagc gaaaacagat ggtaagcggt attcatttca attttgaatt tggcgatgag
    19201 ttgctaagaa cattgttcag ccatcaggaa gaatttcaag atttttccga attcaaaaca
    19261 gaactttatt tgaaaacagc gagaaacttt atgcgctatc gctggatgat cacttattta
    19321 ttcggtgctt caccaatgag tgagaagaac tactttttag atgaatcaca tccgcaagaa
    19381 cctgttcgca gtattcgcaa cagtgcgctg ggctatacga atcatccaaa tgtgaaagtt
    19441 tcctatgctt ctatgaaaca gtatttagca gatatcgagc gaatgatcga agaagggaaa
    19501 ctttcggagg agaaagaatt ttatacaccg cttcgtttcc gaggcggaaa gaaagtcgca
    19561 gatttagcaa caacaggtgt tcgttatatt gaattgcgga atatcgattt gaatccttat
    19621 gcaagattgg gtatcaatcc agagcaagtt cggttcttac aattattcct gatgtacatg
    19681 ttatggacag aagaaaaaga agactgtgac caatgggtag cagaaggtac gactcgaaat
    19741 aacaaagtag cactagaaca accatcagat caaacagaat ttcatcaaga aggtagagaa
    19801 attcttgaag gaatgaagca aatgctggtt gaattggatt ggttggattc tctttattta
    19861 gtagaggaag cattgactca aatggatcat cctgaacaaa cgttagcagc gaaactctat
    19921 caagaagcac agctatcgag tcagcaagaa gttgctgtgg cattgggaca tcaatattat
    19981 aaagaaagcc atgaacgtcc ctatcagttg gccggttttc gcgaaatgga actttctact
    20041 cagatattta tgttcgatgc catccaaaaa ggtgtccaag tcaaagtatt agatgagtcg
    20101 gatcaatttt tgcgtctgca attccaagac catgtagaat atgtgaaaaa tgcaaacatg
    20161 acaagtaagg acagctatat cgtcccactt atcatggaaa acaaaacagt aacgaaaaaa
    20221 gtcttaaaag aagctgggtt ccgggtacca ggaggcgcag aattttcatc catggaagaa
    20281 gcggtaaaag cttatccgag atttgccgag caggcatttg tgatcaaacc taaatcaacc
    20341 aattacgggt taggtatcac gattttcaag gaaggagcta gtctggaaga ttatcaagca
    20401 ggtttagcca ttgcttttcg tgaagacagt tcggttttag tagaagaatt tatgccggga
    20461 acggaatatc gtttctttgt gatcgacgga gaagtccaag ccatcatgtt gcgagtccct
    20521 gctaatgtta ttggtgacag tatccgaaca gtgaaagaac ttgttgaaga aaaaaatagt
    20581 gaccctttgc gtggaacgaa tcatcgtgca cctttagaat tgatccaatt aggtgaatta
    20641 gaacaattga tgttgaaaga acaagggttg acgatcgaat cagttcccca agccaatcaa
    20701 attgtttatc taagggagaa ctcaaatatc agtacaggtg gtgattcgat cgatatgact
    20761 gatgaatttt ctgaagctta caaaaaaatc gccgtttctg cggtagaagc actaggagct
    20821 aagattagtg gtatcgattt gattatacca gataaagaaa tcgatcctac aactgataaa
    20881 aaagcatatg ggatcatcga agcaaatttc aatccagcca tgcatatgca tgtttatcct
    20941 tttgcaggaa aaggcagaag attgacgatg aatgtattga aattgttata tcctgaagta
    21001 ttttaaaaga aaaagctttt gacagattgt cctatttgat gtcaatctga tcaaaagctt
    21061 tttttgcatg taaaaattta tgtgtttttt cgttttaaaa taaacgatcc cagaagcttt
    21121 ttttcttttt tggtttttct ttttcagctg gcgaagagtt ttcttctggg tatttttttt
    21181 cgatatcaat cacaggttca tttactgctt cttttgctgt taagagcaat ccgaattcct
    21241 caggatttgc agtgacgtaa tcgttgacta cagtgaaacg gctattgttt ttcgttgcta
    21301 gctggatata ttggttttgg acagaaaggg gtagagcccc attcaagagt atggaagcat
    21361 ctggatgttt tttgagttct tttaaaaagt tttctttgtt ttttggttgg atcatttcag
    21421 caacagtcat actaagatag cagcgttccc tgaatgtccc taaatattta tgctgttctt
    21481 cagggttcac taaaggtgta ccataaagtc ccttatccaa atggcttttt acatcatctt
    21541 gtgccataaa aaataccctc cttcttctct ttgtctttct tacgattata gcatattccc
    21601 aataatacct aaaacatatg aaaaagcggt tgcttttgtt caacgattca cttaaaataa
    21661 gaatgaaaat cattctcaat tagaaaggtg gattggatgc gctacggacg actagtagca
    21721 ttattagtta ttttaagtag tatttcattg ttcatcggag tatcgaatgt acaattgtca
    21781 gaactgctcc agttagatgg gcaaagctta caaatcttgc tagtgactcg tttgccacga
    21841 acgatttgtt tgatccttgt gggagcgacc agtagtattt gtgggttgat catgcaacat
    21901 ttgacccaaa ataaatttgt ttcaccaacg actgcgggca cgatggatag tgcgcgttta
    21961 ggtattttag tggctatgat tttcctacct ggtgcatcct tgattgtccg atccttgaca
    22021 gcattttgct ttgcttttgc aggaacattg ctttttttat cccttacgcg attgttcctc
    22081 cagaaaaatc aattgatgct tccgttgatt ggagtcatgt tagggaatat cattggttca
    22141 gttgctactt tttttgctta tcaattccag ttggtacaaa atatgacttc atggcttcaa
    22201 gggaattttg cgactgtgat acgaggaaat tatgagttat tgtatgctgt tgttccattg
    22261 ttgctcttgg tttggctatt tgcttaccga tttacgatca ttggtctggg cgaatcgttt
    22321 gcagtaaatc tgggaataga ttaccagaag atgcagtttt taggcattgg cttagtatca
    22381 ttggctagtg cagtcatgtt gatcatggta ggaagcattc cttttatcgg agtgatcatt
    22441 ccgaatcttg tctctagaat atatggagat caagtgcaca aaacaattgg cgtaactgct
    22501 ttagcaggta gtttgttctt agtattttgc gatcttgtcg ctcgcgtgtt gatttttcct
    22561 tatgaaatac cagtcagtgt gatcgttgga atcatcggtg gggctatctt cctttatctt
    22621 ctgataaggg gacgacgata tgcttaaaaa aaagccatct atttttattg gtatgcttgt
    22681 tcttgtttta tttgttacag gaatgtactt gacgatccat acatatggaa actggcattt
    22741 tgcatgtgca ttgaggagta agaaaatttt tgcatttatc cttgtctcgc tagctgtcag
    22801 cagtgccaca atcagctttc agacaattac gcataaccag tttttgactc cttcagtatt
    22861 gggacttgag tcgttatatg tccttgtcca aacggtcctt ttcttcttta tcggaggagt
    22921 gacgatgctt cagcagacag gtacagtttt tttcttattg aacgttgggc tgatggtcgg
    22981 gctgagtcta ttgttatttt tctttctttt gaaaaaaatg agagcgaatc tttttttatt
    23041 actgatgtca gggatgattt taggtacgct ttttggtagt gtcagtacct ttttacaagt
    23101 catcatggat ccgaatgaat atgatttgct tcaaggcaga ttatttgcaa gctttggcaa
    23161 tatccagagt cagtatcttt tgcctgcttc tttgatcatt atcggctgct ggtacctgtt
    23221 atttcgatat cgacatgtgc tagacgtttt ccatctcgga aatgaacaag cagtaaacct
    23281 cgggatagac acagatcgtt ttgaattttt gattttatgt tgtgtcagtc ttttgacagg
    23341 gacatcaacg gctcttgtgg gtccagttac ttttttaggg tttattgtcg caaatatcag
    23401 ttatcgtgtc ttttctactt atcgtcatgg tcagctattt atcggaagct ttctggtagc
    23461 tatgctgttt ctgacaggag gacagctcgc agtagagcag attttcggct ggaatacaac
    23521 agtgagtgtc gtcgttgaat ttgtcggagg aatttatttt gtcacaaaat tgattcgcga
    23581 aaggaagagt atcctatgat tgatttacaa aaggtcaata aactgtacca tggaaaatca
    23641 gtggttaaag atgtacagtt aacgattcct gaaaagaagt tgactgcttt tatcggtcca
    23701 aatggtgctg gaaagtcaac ggtgttgtcg atgatcagtc gtttgatacc aaaagataca
    23761 ggtgaaatct atttagacca taacgaagta aaggcttgga aatccaatga attagccaaa
    23821 aaattatcga tcctacgaca atcgaatgaa ataaatgtcc aaatcaccgt gaaagaatta
    23881 gtagaatttg gacgatttcc ttatacgaaa ggaaaattga cgacaaaaga cgaggaaatc
    23941 gttcaggaat cactggaaca ccttgggtta gaagaaatag cggatcaatc gattgacact
    24001 ctttcaggcg gtcaactcca acgtgcgtat attgcaatgg tgctagcgca agacacggat
    24061 tatattttac tggatgaacc tttgaacaat ctagatatga attttgcggt tcaaatcatg
    24121 cagattttaa aaaatttagt acaagaaatc gggaaaacaa ttgttattgt tcttcatgat
    24181 atcaactttg ctgcaagtta tgcagacgaa attgttgcaa tgaaagatgg acagttattt
    24241 acgcaaggaa cgacccaaga aatgataaaa aaagatgtat tggatgaatt atacgaaatg
    24301 gacatacgga tttgtgagat tgaaggccgc agattctgtc tttatttcta aaactaacat
    24361 taggagagaa gagatgaaaa aattagtagt aggtatcttg attctagcat catttggttt
    24421 agcagcatgt ggaaatagca acaacaccag tcaggcagac acaaaaaaaa gcagcacaca
    24481 aactgaaact acattgacga taacagatag taatggagac caaatcgagg tgccaaataa
    24541 tccagaaaaa gtcgtcgtat tcgacaatgg ttctttagat acgatggatg ctcttggtgt
    24601 tggagataag gtagttggag cagctacaag cagccttcct gagtatcttt catcctataa
    24661 aaaagtagaa tcagcaggtg gaataaaaga accagactta gaaaaaatta atcagcttca
    24721 gccagatcta atcatcattt ctggccgaca aagagatttt caaaaagatc taagtcaaat
    24781 tgctccgacc attttccttt ctttagatgc gaaaaatcca tgggaatctt tccagcaaaa
    24841 tgtcactgct ttaggggaga tctttggtaa gcaagaagaa gctaaaactc aattagagga
    24901 attatctagt gcaatcgatc agacgaaaaa gaaagcagaa gctactgaca aaaaagcact
    24961 agtcacactt gtgaatgaag ggcaattatc agcctacgga tctggttcta gattcggttt
    25021 catccatgat ttatttggct ttgaacaagc agatgatcaa atcgaagcat ccactcacgg
    25081 tcaaagtgtt tcttatgaat atgtgctgga aaaaaatccg gatatcttat ttgtagtaga
    25141 cagaacaaaa gctattggtg gagacgacag caaagatgat atttctgcca atgaactggt
    25201 agcacaaacg aatgctggta aaaatcagca gatcatctcc cttgaaccag atgtctggta
    25261 tttaagcggg ggcggtttag aatcaatgaa actgatgatc gaagatgtga atcaagcatt
    25321 taaatagcat aaataacaga ggattaaact ctcatactat gtaacaataa tgtttcttac
    25381 attcttaaat agcaagagcc tatcctcaaa gaagttatca aaaaccatcc gtaaaatcgt
    25441 attgatgatt ttacggatgg tttttattta tttgagaaat cttttttcaa cgtgaattca
    25501 ttatctacat catacgggtt gattctagaa tggggagtgg caaaaaatag tgtgatccat
    25561 tttttcagca tcaagatcgc ctcctttgat ttgaatcaaa ggtacaaagc gaaacttaga
    25621 taaaacttaa atttagatat ttttattttg taccgatctc ttctttaaac gatgttccat
    25681 ctgctgcttc tcggcaataa gtccactaga ctagaaaata tgagaagcta ttttctgtcc
    25741 agcgttctta ttactcaaaa caaatcgcag ttgtcacaat ctcttctaaa cgatgttcca
    25801 tctgctgctt ctcggcaata agcccactag actagaaaat atgagaagct attttctgtc
    25861 cagcgttctt attactcaaa gcaaatcgca gttgtcacaa tctcttctaa acaatgttca
    25921 atcagctgac cttcggtatt agctcaaagc actgatttca caacctctat ttaggcatgt
    25981 gtctcagcaa gcggttgata gttgatgatc tgcatttttc gataaaaatc ggcgggaagc
    26041 ttactccacc gttttagtaa ttgatagaaa taactctctt ctgctttgat tttatactcg
    26101 cttaatatca atccaaaact attcgttttt tttgaagcta attttttata aatatccaat
    26161 gctttggttt tatcttcatt gacaaagagt gtttttactt caatataaga accctcgata
    26221 ttttgtgttt cttcgataaa atagatgttt tttatatatt ccttcatagt gattcctcca
    26281 aagaagtctt ttatttgata agtttactct accgaagagt tgttaataat ccgtgaacaa
    26341 aaagtaattc tttttccaat agttgttaaa ggttttttat tttttatagt attttttgtt
    26401 atttaagaaa ggaataaggt taaacaaata acaggttgct ttttgggtct ttttccgttc
    26461 aagaaactga acgaagaaaa gggaaagttg tctcttttgt gataagagaa atcataataa
    26521 acgaatgact tactttccaa taaattgaga acagtcagaa ccagacaaat tagttgcatc
    26581 tgatgaataa agtgattatt ctatgacatg aggtgataca gatgaaatta gaagtgacaa
    26641 aagaagcaca agaagtattg aagaataaat tagaagcaga cgatcaactt ttattagata
    26701 tcgaaaacgg tgacggtcca tttgcagaaa gccaagtcag ttgccaattg gatacggcat
    26761 ttcgattgat cattgtcaaa aaagatacac aaacagatct aagtctttat tctgtagtgg
    26821 ctgatacacc agttggacca atcaaaatca aagacagtgc aatactttac atggatgatc
    26881 catccacact tgctttagaa ccaacttata atagtttaca attaaaagga ccaagcggct
    26941 tgagaaaagg caacttgcaa gttgttcgaa aagactagag ggataaaatg ggaaaattgg
    27001 cgatcatcag cgacctacat gtggacatca ataaattcgg cgaatcagag ctgatgcagc
    27061 tttggcaagt tttgcaaaag cacaagatca ctcggcttca tctagccgga gatacggcta
    27121 ataaagtaga ccgttgccta gctgttgtcg aatttttcgt caataaagga ttatctacga
    27181 catttcattt cggcaatcac gagttggcag atgtgcccaa tgaacagatg atggaccatt
    27241 ttccagaccc acatttttta aatgaacggt atattgcttt aaatgagcag accgttcttt
    27301 tagggatgaa tggctggtac gactatcagt tttccgaatt gaaagattac gaggcaatcg
    27361 tacgaatgaa gcgtttgtat tggtatgatc ggttcatcca gcgtgggaaa acagatctcg
    27421 aggtcaatgc agaaatgctt gcagctgcaa aaaatttgct agaccagctg gaagcaaaaa
    27481 aacttgaggt gattttatcc actcatttcg ttcctaaacg ggcatttatc atgtatcaat
    27541 ctgctcctta tgagcgttgg aacaaattaa atgcattttt aggatcagaa tcattcggag
    27601 aattacttga ccgttattca aatatcaaac aagtagtttt tggccatacg catcgtcgct
    27661 ttgaagataa atccatcggt cacacgattt acagctgcag accttttggt tattattatg
    27721 aatggcagct gacccgagat tttgtcttga aagaacagtt gctagacagc tatgatccta
    27781 tgaaattacg tgtcctcttg cgtgcccatc acccagcttt tcaggcctat caaacacaac
    27841 atttgcaaga ggaatttgaa caagcgatga caatcgttcc gtattgagac agcattagta
    27901 atgtgtaaaa gaaaatacct tgtaaaacat aaggataagt aagagcagta gatccattta
    27961 tcgtggatat gctgcttttt ttgcggaaaa taatcagcga atgtttattt gatcggatga
    28021 tttgtaactc gcagagtact agatgcaagc gtgtattctt tttttgaacg ataatcacaa
    28081 aaaaaaatat aatatacatt ttttatttaa ttataattaa tattgattga agtctaggca
    28141 gtcaagtcac attttgatag ataggagggg gacttacgaa ttattttaaa ggctatgaca
    28201 gctgtatttc tagattgtgt ttgcatcaag ttgcaataaa aactagaaat caaatcgggc
    28261 tgataatcat ttataactaa tcgatacata ggaccaaata taggtctatg ttcttttatt
    28321 tcataagtat cgtaaaacag aattttgcta tggattaatc caatcgactg atatgtttag
    28381 taaataaaga aagaggaatg tcaatgatga aaaaagaaat cgtattaaca gcagcaatga
    28441 tgttattcag catggtagca agtacgactt ttgtttctgc aactgaagta tacccaaagg
    28501 aatataacac agaaggaacg attacatttg aagcaggaga tgagggtgta actcctcctg
    28561 ttgatccaga gaatcctgat cccaataaac ctgtcgatcc atcagatcct ccaagccctg
    28621 gaacaggagg ggcattatcg attgattatg gatcaaaatt caaatttggg actcaaaaaa
    28681 tttctactgc ggataaaacg tattatgcag ctgctgatgt gatgaacgat gggtctagaa
    28741 aaccgacata tgtccaagtg acagatagga gaagtacctt atctgggtgg aaattaagtg
    28801 tctctcaacc agaacagttt aaaactgcca gtggggatga actagttgga gcacagctga
    28861 aatttacgaa agggcaggct gtttctttgg ttgatccaac atatacgccg caaacggtga
    28921 attctgaatt aacgttgaca ccgggaggaa acaatacgtt agcaattaat gctaaatcag
    28981 gaacaggtgt ggggacttgg gtctatcgat ttggtgcaaa tgaaaatgaa aatcaagatg
    29041 cggtacagct ttcggtaccc ggtaaatctg tcaagttagc gcaacaatat tccactaaat
    29101 tagtttggac attagaagat acacctaaca actagtttac tgaaaagaac tgttaaaaat
    29161 aaatacgtag tcatattgtc ttgcgaggta atcgagcaaa aattgtcaaa ggctaggcta
    29221 aatttgtttg gatacgaata gttcatctag gaatttaacg atcaggagga aaagatatat
    29281 tgaaaaagag gaggatcgtg ctcattttaa gcagtctctt agtgactaat atctttccga
    29341 tcatagggta ctctttggaa ttgaatcagt tggagcatgg aaatagaaga gacgaagaaa
    29401 gcaaactcac ttcgacattc gacagtttaa tagaatccaa ccagacacaa gaaagcagtg
    29461 aggatttaag tatttcgagt agtggagaga ccactgacaa cgtagaagct gagggttcgg
    29521 acaaacaagg actagaagat gcaccaattt ctgcggttga gctgcctgac gatcccaata
    29581 ttatttctat cgataaaatt tttcaaacac ctatcggtac tagcacgtcc atattggaaa
    29641 atgggaagtt actgcagttg aacccagcag aaaggtctca aagaggagct atttggtcaa
    29701 ataagaagat tagtttactc tcggatttta catttaaatc ttatttatac ttaggaaatc
    29761 aacgagatag agcgggagat ggcatgacct ttacattgac tggcgatcct agaatggcta
    29821 gtaatcccag tcaagttatt ggatcgccag gtatgggaat tggctcatat tctactcgtt
    29881 ctggccaacc ttacattgcg aatgcgctct cgattgagtt tgatacgtat aaaaatagtg
    29941 gttcttctga tcgaatggat cgagagataa ctgctgataa tagagggcat ggacatgtcg
    30001 cttttgtcac gcctaaacca aataataata attattctgg agagcatact ggcgtgacgg
    30061 tagctcctac atttctttct aatggcacat ggcgcttgtt aataattaaa tgggacgcgg
    30121 agcaaagaag tcttacttat gatttggaag gtgtcggtcg ctcttctcgg attatacctg
    30181 atctcattag gcagtttggt tctacagaag taaattgggg gtttacttct tcaacaggta
    30241 ctttttttca agaaaatgct gtcgctgtca cgcgacttcc atcgaatgtc acatcaacag
    30301 ctgatgtaaa agtcaacgat agtgattatg gggccatcgc tgaagctgga ggaaacgata
    30361 agataactat cagaaataca ttgaaggtcg agggggattt tcttgaatat aagaaaatgc
    30421 agactagtat cgacctccca caagaattaa ctattcccga gcatatcttg atcaatggaa
    30481 atcaaattga tgccaaagat atcttgataa ccggaaatcg agtcacgcta aatctgacag
    30541 attattttat tgctggagat acgttaataa tcgaattgga gacaactttg caggaagtaa
    30601 ttcctgaaaa agtactcact atgcattttg aataccttga aaacgggaat ttgattgagc
    30661 aatcgaatga aataaaccta aagatcgcaa agctgaagga gatagatatc catgtttatt
    30721 ataaagataa agacagtcag attgatattg ctgaaatgaa aacaatcact gggaaaatag
    30781 gagagaagta taaagaaact cctattgata ttgaagggta tgtttttgac tcggattcag
    30841 cgaatacaga aggcatcttc tccgaaaata cagaggatat ctatttttat tacagaaaag
    30901 gcaaacttta tctctcaact gctcctcaat atgtcaattt tggaaaacat aaaatatcca
    30961 gtagaccatt aagtgaattc ggacagtttt caggtgggtt gaaaattata gatgaacggg
    31021 cttttggaag ttggaaatta caattgaaac aagagaaagt tcttacaaat ggagattttg
    31081 agcttccaga agctctttcc tttgttacct ctgaaaatac aacggttata gggacatccg
    31141 ctgttacgat tttcgaaagt gatcaaaagg gtgaaagcga tttgtcctct cttttagatt
    31201 ctacagctca tcgtggaatc agaataaata ttcctgtaga aaaccaaaga atcggaacct
    31261 ttgaggggaa actatcttgg atacttgcag atgtcccagg aaattaacag gaagagaaga
    31321 atataacaaa attattgagt ttcaagaagg gcacaggtac cataggctct tgtgtcccac
    31381 agagacaaga ttttgttata gtcttctctt acatatgatt aaaggagcga ttttcttgag
    31441 accaaaacaa caattattta taacactcct agttatttta ggggcacttt ttaacaatat
    31501 atcagtgtca gctagtgcat ttaatttttc agtgacgccg ataacttcag agaatcaaat
    31561 cgacaagaga aaaacttact tcgatcttca acttgatccg gatcaggaag tagaagtaaa
    31621 agcggaattg agaaatgata cagaaaaaga agtgaaaatc gatatttctg tgaatagcgc
    31681 aaccaccaat tcaaatgtga tggttgaata cggaaaaaac gagattgaaa aagacatgag
    31741 cctgatcttt gatctggtag attatgtgag ctatccacag atagtgacgc taaaacctaa
    31801 aagtgtccaa accgttgctt ttcatgtcag gatgccgaat gagcgatttg atggtgtatt
    31861 ggctggtggg atcacattcc aagaaatcca gacggaaaag gaccaaacag aaactaagga
    31921 tcaaagctta tcgattgaga atgaatacac ctatattatt gctttgttga tgcaacagac
    31981 actgaacgaa gttgctccga accttactct tcatgaagtt aaaccagatc aaatcaatgc
    32041 aagaaatgtc atcttagcaa acgttcaaaa tgatcaaaaa acctatatca atcaagtagt
    32101 tattgaaaca aaaattacaa aaaaaggaca ttctgaagtt ttatatcaag aggaaaaaga
    32161 aggattgcag attgcgccga atacaaattt ttcttttcca actgctttaa atggtcaacc
    32221 gctaacccca ggtgaatatc accttacaat gactgtttta ggaaatgaaa atgaaaatgg
    32281 aaaattttcg agaaaaaaag gaaatactac gatcaactat accaatcaat gggtatttga
    32341 aaaagatttt acaatagacg gaaaagtagc caaggaattg aacacgacgg atgttacgat
    32401 taaaaaagac aataattgga tttatatctt agtagcttca cttttcttat tgtttatttt
    32461 attatgcata cttttgtttg ttttgagaaa aaaagaagag aaagaaaagt aaggttaaga
    32521 taacaaatag taaattttta ttattgtagg atggttgggc tctttttata acaatataaa
    32581 atatacca
//
LOCUS       DAJSKS010000012        32372 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJSKS010000012 DAJSKS010000000
VERSION     DAJSKS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.012"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..391)
                     /locus_tag="ON396_000542"
     CDS             complement(<1..391)
                     /locus_tag="ON396_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCR3112675.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQ"
     gene            470..901
                     /locus_tag="ON396_000543"
     CDS             470..901
                     /locus_tag="ON396_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HCR3112676.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(1000..1233)
                     /locus_tag="ON396_000544"
     CDS             complement(1000..1233)
                     /locus_tag="ON396_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HCR3112677.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            1416..3086
                     /locus_tag="ON396_000545"
     CDS             1416..3086
                     /locus_tag="ON396_000545"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCR3112678.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            3233..3619
                     /locus_tag="ON396_000546"
     CDS             3233..3619
                     /locus_tag="ON396_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112679.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            3606..4304
                     /locus_tag="ON396_000547"
     CDS             3606..4304
                     /locus_tag="ON396_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR3112680.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            4309..4773
                     /locus_tag="ON396_000548"
     CDS             4309..4773
                     /locus_tag="ON396_000548"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR3112681.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            4770..5681
                     /locus_tag="ON396_000549"
     CDS             4770..5681
                     /locus_tag="ON396_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR3112682.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            5916..6455
                     /gene="pyrR"
                     /locus_tag="ON396_000550"
     CDS             5916..6455
                     /gene="pyrR"
                     /locus_tag="ON396_000550"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCR3112683.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            6469..7752
                     /locus_tag="ON396_000551"
     CDS             6469..7752
                     /locus_tag="ON396_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR3112684.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            7765..8691
                     /locus_tag="ON396_000552"
     CDS             7765..8691
                     /locus_tag="ON396_000552"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCR3112685.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            8710..9990
                     /locus_tag="ON396_000553"
     CDS             8710..9990
                     /locus_tag="ON396_000553"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR3112686.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            9995..11071
                     /gene="carA"
                     /locus_tag="ON396_000554"
     CDS             9995..11071
                     /gene="carA"
                     /locus_tag="ON396_000554"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR3112687.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            11076..14261
                     /gene="carB"
                     /locus_tag="ON396_000555"
     CDS             11076..14261
                     /gene="carB"
                     /locus_tag="ON396_000555"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR3112688.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            14315..15091
                     /locus_tag="ON396_000556"
     CDS             14315..15091
                     /locus_tag="ON396_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HCR3112689.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            15088..16020
                     /locus_tag="ON396_000557"
     CDS             15088..16020
                     /locus_tag="ON396_000557"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HCR3112690.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            16017..16733
                     /gene="pyrF"
                     /locus_tag="ON396_000558"
     CDS             16017..16733
                     /gene="pyrF"
                     /locus_tag="ON396_000558"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR3112691.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            16740..17369
                     /locus_tag="ON396_000559"
     CDS             16740..17369
                     /locus_tag="ON396_000559"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR3112692.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            17357..17671
                     /locus_tag="ON396_000560"
     CDS             17357..17671
                     /locus_tag="ON396_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112693.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            17709..18350
                     /locus_tag="ON396_000561"
     CDS             17709..18350
                     /locus_tag="ON396_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3112694.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            18393..19085
                     /locus_tag="ON396_000562"
     CDS             18393..19085
                     /locus_tag="ON396_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HCR3112695.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            19082..19984
                     /locus_tag="ON396_000563"
     CDS             19082..19984
                     /locus_tag="ON396_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3112696.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(20142..21848)
                     /gene="efbA"
                     /locus_tag="ON396_000564"
     CDS             complement(20142..21848)
                     /gene="efbA"
                     /locus_tag="ON396_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HCR3112697.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            22038..22661
                     /locus_tag="ON396_000565"
     CDS             22038..22661
                     /locus_tag="ON396_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112698.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            23111..24160
                     /locus_tag="ON396_000566"
     CDS             23111..24160
                     /locus_tag="ON396_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3112699.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            24157..25038
                     /locus_tag="ON396_000567"
     CDS             24157..25038
                     /locus_tag="ON396_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3112700.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            25041..25811
                     /locus_tag="ON396_000568"
     CDS             25041..25811
                     /locus_tag="ON396_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3112701.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            26090..26944
                     /gene="aroE"
                     /locus_tag="ON396_000569"
     CDS             26090..26944
                     /gene="aroE"
                     /locus_tag="ON396_000569"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR3112702.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            27122..28285
                     /locus_tag="ON396_000570"
     CDS             27122..28285
                     /locus_tag="ON396_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR3112703.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            28402..28812
                     /locus_tag="ON396_000571"
     CDS             28402..28812
                     /locus_tag="ON396_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3112704.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            28986..31214
                     /gene="topB"
                     /locus_tag="ON396_000572"
     CDS             28986..31214
                     /gene="topB"
                     /locus_tag="ON396_000572"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR3112705.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            31237..31542
                     /locus_tag="ON396_000573"
     CDS             31237..31542
                     /locus_tag="ON396_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112706.1"
                     /translation="MFIPKSENIAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTL
                     ILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            31702..31938
                     /locus_tag="ON396_000574"
     CDS             31702..31938
                     /locus_tag="ON396_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3112707.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            31935..32334
                     /locus_tag="ON396_000575"
                     /pseudo
     CDS             31935..32334
                     /locus_tag="ON396_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
BASE COUNT        10588 a         5640 c         7125 g         9019 t
ORIGIN      
        1 attgtttatg gagagcctga cgtgcaagat tatctgctcc tttatttttg ttttcgggca
       61 accattgcag gatcaataaa gaaaattcgg atagaagttc ttggattctt tgaaggtagg
      121 gaaggaagag ctcgttcttc gttgcgtttt tgtcgaccgt agaaactagt gttttgctgt
      181 ctgaaaaaca gaaaatcgtt tcattctgta agtcgttttt ctttagaaat tctaaagttt
      241 ttaaaaagac ttcgaactct gcttgatggt ttgtccctat agaaagcggg atagatagct
      301 gttcttgttt accttgatag acaatcaaga ttccacctcc actaggacca gggttgcctt
      361 ttgtagaggc atcaatgtat acttttaaca taaaaagaga ttctcctttt tgttcaaact
      421 aatgttatgg taagattata tcattactga atcggtgaga ggttagagaa tgaaaaaaaa
      481 tattcgatgg cagcctgaac tggcacatac gatcatctat tggtcattga ccttgatcat
      541 tctgtttttt agtctgattc tttctttaga aaacacaggt ccttattgga aaagcaattt
      601 tgttttaggt atttttcttg tttttctatt tctaggtttg cggcgttctt ttattttaaa
      661 atcaaactct gtgattatac gttatgctgc tttttggaaa gaccgagaaa tatctgtcgg
      721 acagattcga tctttgacgc caactaatac tggggtgcaa atgatgttgc aagatgagaa
      781 agccccatct gttttcatga tgcgaaaaaa agatcaagta gtcctttttg atcattttga
      841 aaaaaacgga cccaaatcag aatcaattcc ttatccttat acgaaaaaaa gaaaaagcta
      901 attttaaaaa tcagaaccta aaaaaacgaa tttttacagg aaaagaaaag agtgagacgt
      961 acggttcatc ccgtttagcg tctcactcta tttgtttatc tattcatgca tactagtatc
     1021 ggtagtctga acgttgcctt tcttgacatg ggcagcttgt gttcctcgat tcccttcgat
     1081 gatctcaaat tcgaccactt gattttcttc caaagttttg aatccctctt cttcaatcgc
     1141 agtaaaatgt acaaaaattt cttctgtgtc atcataactg ataaagccgt atcctttttt
     1201 gttgtcaaac cattttacaa ttcctgtggt caaagcactc acgacctttc taaagtctga
     1261 tcatagcctt cattataaag agtatttata agtacgtcaa ataaattaaa aacctgtaat
     1321 tttatgtttt ttttctgaat aaaaaaaggt ataattggca tatcttaaca aagaagatat
     1381 tttgttgaat aaagttcgtg aaaaggggta atcaaatgaa gaatgatctg caaatttcac
     1441 aagaaacaga gctagaaccg attgttgaag tggctagtaa attagatctt aaggaagacg
     1501 atttggaact gtatgggaaa tataaggcga aaatcaattt ttctggaatc aatcgatcaa
     1561 aggataacgc tgaggggcat ttgatcttag ttacttccat caatccaaca ccagctggcg
     1621 aaggaaagtc aacgatcact gtagggttag gagacgcgct caataaaatc aataaaaaat
     1681 ctgttattgc attgcgagaa ccctcacttg gtcctgtgat gggaatcaaa ggtggtgctg
     1741 caggcggagg atatgcacag gtattgccta tggaagatat caacttgcat tttactggtg
     1801 acatgcatgc tatcacaaca gctaataatg cattatctgc tcttttagat aatcatattc
     1861 atcaaggaaa tgaattgaac atcgatgccc gccgagtgat ttggaaacgg gtagtcgatc
     1921 tcaatgatcg tgaactgcgt aaagtaattg ttggcttggg tgggccaatc caaggtgttc
     1981 caagagaaga tgggtttgat atcacagttg ccagcgaaat catggctatc ttgtgtcttg
     2041 cggcagacct tgaagattta aaggctcgtc ttgcaaggat cgttgtcggc tatacatttg
     2101 accgtcagcc tgtgactgct ggagacttga aagcagaagg cgctctggca ttactgctga
     2161 aagatgcaat caagccgaat cttgtacaaa caatttacgg aacaccagct tttgtccacg
     2221 ggggaccgtt tgccaatatc gctcatggct gcaatagtat tttagctacc aagacagcat
     2281 tgagattagc agattacgtt gtcacagagg caggatttgg tgcagatctt ggcggagaga
     2341 aatttttaga tatcaaagta cctaacttga aaaaagcgcc ggatgctgtg gtgattgtcg
     2401 ccactgtccg tgcattgaaa atgcatggcg gaatgaaaaa agatgaactt aaaaatgaga
     2461 atcttgatgc tttgaaagta ggttttgcaa atttaaagcg ccatatacgt aatatggaac
     2521 aatatcagct acctgtgatc gtcgcaatca atgaattcgt aacagataca gacaatgaac
     2581 tcacactgtt ggagcacttg tgtgaaaatc aggggattct tgccaagaga gcgagtgtat
     2641 gggcagatgg agcagaaggc ggcgttgatt tagcaaaagc tgtagtacga ttgattgata
     2701 gaaaagaagc taattacaag ccattatacc gcctagaaga aacgatacaa gaaaagacag
     2761 aaacaatcgt caagaaaatt tacggaggaa atggtgttgt tttcagcaaa aaagcgcaaa
     2821 aacaaataga agagtttacg aagaatggat gggacaaact gccgatttgt atggcaaaaa
     2881 cacagtattc attttctgat gatccttcac tgctaggtgc accggaagat ttcaccatca
     2941 cgatccgcga aatcatccca aaattagggg caggattttt agtagccttg actggagatg
     3001 ttatgacgat gcctggtttg cctaaaaaac ctgctgcatt gaatatggac gtgacaaatg
     3061 acggagaagt gttgggattg ttttaagctt gattataatc taccatcgaa ggagctgtat
     3121 tttgactaca gcttcttttt ttagacgata gggaattaat tttgtttaca ctaggatcat
     3181 tctttttctt tattgtattt tgagctatac taaagcataa taggagggta tgatgaaaac
     3241 gacatttata agaaaaaccg gtttttacgg tatgggtagc tcgattcagc taagaatcaa
     3301 caatgaaaaa cgcagtgttc tcagtcatga acaaaagata acgcttgatc tggaagtgcc
     3361 ctttacgatg caggtcacct tttattggct aaaaagcccc gtttatacag tgaaagaagc
     3421 aaagcctgct tatgtgatca caatgaatta cttgattctt caaatctatc cgtttttatt
     3481 tctagcaact gggttggggg ccgttttcct acaaaattta tttttcagtt tttttgcagt
     3541 tattgtgttg attgcgttct ttttctatat aaaaaaccgg gcatatttga tcaaggagga
     3601 aaattatggc aaactctgat ctttttttaa tcagtttcaa tcgaatcgaa aaatggatga
     3661 gagacgagat gggaaatgca cgaaatatgg gatttaccga acttgtcaga cgcctagctc
     3721 aaagaaaaca attaatgatc agaaaatacg aagatgatct tttacagctt gctcagttaa
     3781 ggaatgcgat cgtccatgat cggatcgcag tcgattttgt cattgcagag ccaaatgatt
     3841 ggacagtcaa gcgaatccag ctgatcgaac aagaactgat tcagcctgaa accgtgctgc
     3901 ctcgatttgc aaaacgtgta acaggttttg aacaagatct tccgttactt gaactgttaa
     3961 agatagtagc agaaaaaagg tattcacaat ttccgttata caataaagga aaatttgtag
     4021 cattgattac tttacgtaat atcgggtttt ggctagcaaa agaaagccaa aaaggtccgg
     4081 ttgatttaac aaataaacga gcgttggatt tgatcatcca aaacggaaag tacacaaatt
     4141 atcattttgt tccagctaaa acacatattt acgaggttga agcgatgttt cgtgagcaag
     4201 ggactttgga agccgtactt atcactaaag atgggaatcc cgatggtaat ctgctcggga
     4261 tcgtacgacc tcgtgatatt tataaacaaa tagaaaagga ttgatttttt gttaattatt
     4321 tattggatat tcagcgcttt gctagtagga gtggatcagt tggtcaaatg gtggatcact
     4381 gataatctag cgcttggaga gacgaaaaat cttataccag gtattttgtc actgaatcat
     4441 attcgaaaca ctggagccgc atggagtatg ctagaaggga agatgtggtt ctttactata
     4501 gtgacactca ttgcagtcgt tgtgattctc acattgatga ttaaaaatcg tgaaaaaggt
     4561 aatcgctggt tcatgattgg attgagtttg attctagctg gtgcgattgg caattttatc
     4621 gaccgtattc gtcttggcta tgtagtagat atgttccaga cagattttat gagctttccg
     4681 atttttaacg tagcagatgt gacacttgta tgtggagtga tttgtatgtt aatttatatt
     4741 attttagatg aaaaggatca aagaaaaaaa tgacagaact taaaacgacg attcaccatg
     4801 aatctggtag gatcgataaa gtattgacta atttattcac cgattacagt cgttcgcaaa
     4861 ttcagctgtg gctgaaaaat ggagcggttt ctgtcaatgg acagccagtc aaagcaaatt
     4921 acaaagtaaa acaaggagac gagattactg tcttgatccc cgaaccagaa acattagatg
     4981 tggtcccaga aaatatcccg ttgacgatcg tttacgaaga tgaagatgta gcggtgataa
     5041 acaaacctca aggaatggtc gttcatcctt cagcaggtca tacgcatggc acaatggtca
     5101 atgcgctgat gtaccatatc aaggatcttt ctacgatcaa cggcgttatt cgtccaggta
     5161 ttgttcaccg aatcgacaaa gatacttccg gactgctaat ggttgccaaa aatgaccagg
     5221 cacacgaatc attagctcag caattaaaag ataagacctc tttgcgcaaa tatgttgctt
     5281 tagtccatgg agagattcct cacgaaaaag gaaccatcaa tgcgccaatc ggccgttcga
     5341 aggaaaaccg gaaaatgcag gcgataagag aagatgggaa gtcggctgtc acacatttta
     5401 ctgtattgga acgtttcgaa ggatttactc tggttgaatt aaaactagaa accggaagaa
     5461 cacaccagat ccgtgtgcat atgaagtata tcggttatcc gttagctgga gatccagtat
     5521 acggaccgaa gaaaactttg aaaggcaatg gtcagtttct ccatgcgcaa atgttaggtt
     5581 ttacgcaccc gactacaaac gaaaagatga tctttgaagc acctcttcca gaagtttttg
     5641 aaaaaacttt agaaaaatta agaaaaaaca ttgcatttta atttagataa cggtatagta
     5701 attaacaacg aaagcataaa acttaataga attcctttaa gtttagtccc gtgaggctaa
     5761 gaagggtggg agcaaggata cgtatggtgc gccataagta gattgctcat tgacatagta
     5821 tagctatcag tgtatcagta tacgctcagt ctaaacattc ctcctgtcct cttagggcag
     5881 gagttttttt atgcaaaaaa gtagggggaa tgaaaatgca agcaaaagaa gtagtagatc
     5941 aggtaaccat gaagcgagca ttgactcgaa ttacttatga gattatcgag agaaaccata
     6001 gtatccaaga tatcgtattg gtaggtatca aaacacgagg aatctacatt gcatcaagaa
     6061 ttgccgaacg cctgaagcag ctagaagata tcgatattcc tgtaggagaa ctggatatca
     6121 ctttataccg agatgacaaa aaagaaaatc cagaagaacc ggaacttcat tcttctgaca
     6181 tcccagtttc cttagaagga aaagaagtta ttttgattga tgatgttttg tatacaggac
     6241 gaacgattcg agcagcaatg gacgcagtaa tggattttgg cagaccaaga aaaatttcat
     6301 tagcagtcct ggtcgatcgt gggcatcgag aattgccaat ccgtgcagat tatgttggga
     6361 agaacattcc aacatctcgg gcggaagaga ttttagtaga gatgcaagag cttgatggac
     6421 aagatcgcat catgattttg aaagaggaag attagaggag agagaaacat gtcggagaaa
     6481 aaaggatttc ataatgatga cgtggtgtta gatatccacg atcgcccaac cccaggacat
     6541 tgggtaggat taagtttaca acacttgttc acgatgttcg gagcaaccgt gttagtcccc
     6601 atcttagtag gtatcgatcc aggtattgcg ctagtcagtt caggactagg tacattggtg
     6661 tacttattta ctactaaagg aaagatacct gcgtacttag gaagcagctt cgctttcatt
     6721 gcagcgatgc agcttttgat gaaaacagat ggttatccag cgattgcaca aggagcagtc
     6781 acaactggat tagtctattt gattgttgcg ctgatcatca aaaagatcgg ttcagcatgg
     6841 ctggataaaa tcttgcctcc aatcgtcgtc ggtccagtca tcatggttat cgggttggga
     6901 cttgcggcaa atgcagcaac aaatgctatg tataatcagg gcacgtatga tttcaagtat
     6961 attgctgtag cgctgatcac attaggatta acaattttct tcaacatgtg gctaagaggt
     7021 tttctcggat tgattccgat tttactaggt attattagcg gatacatcgt agccttgctg
     7081 tttggaatcg tggatactaa gccaatcgta gacgctgcct ggtttgctgt tcctaacttt
     7141 gagattccct ttgtacaata tgaaccaaag ctatatcttg gagcaatcac aacaatggca
     7201 cccatcgctt ttgtgacgat gacagagcat atcggccatc ttatggtatt gaataaacta
     7261 actaaacgta atttctttga agaaccgggt cttcataaga cgttgacagg tgatggatta
     7321 gcacaaatcg tggcagcctt tgtcggaggt cctcctgtaa caagttacgg tgaaaacatt
     7381 ggtgttctgg ctatcacacg tgtccatagc gtgtttgtga tcggtggagc tgcagtattt
     7441 gcagtcattt taggattcgt aggaaaatta agcgctttga ttttaagtat ccctggacca
     7501 gtcatctcag gaatcagttt tatcttattt ggagtaattg ccgcaagtgg aatgaagatt
     7561 ttaatcgaga acaaaatcga ttttgataaa aagaaaaatc tgttgattgc ttcagttatc
     7621 ttagtagtag gaattggcgg actcgtcttt gaagtaggca catttacatt gtcggcaatg
     7681 gcattagcaa ctgtacttgg catcatttta aatcttatct taccagaaac ttcacgaagc
     7741 gaagaaaact aaggaggaaa cattatgatc atcaaatcag aacgtatcag cttaaagcat
     7801 ttattgaccg ttgaggcact tactgatcaa gaagttatgg gcttgatccg cagagggcag
     7861 gaattcaagc aaggtgctaa ttggactccg caaaagcgcc agtactttgc tacaaacctc
     7921 tttttcgaaa atagtactcg cacacataaa agcttcgatg tagcggagaa aaaactaggg
     7981 ctagaagtga ttgaatttga agcaagcaca agctctgtcc aaaaagggga aacactatac
     8041 gatacagtac tgacgatgtc agcattaggc gtagacgtag cggttatccg tcatggtgat
     8101 gagaactatt atgacgaact gatacaaagt aaaacaatcc agtgttcgat tatcaatgga
     8161 ggagacggaa gcggacagca tccaacccaa tgcttgcttg atttgatgac catttatgaa
     8221 gaatttggtc attttgaagg tctgaaagtt gccatcgtag gtgatatcac tcactctcgc
     8281 gtagctaaat ccaatatgca gatgttaaaa cgtctaggcg cacaagtatt cttctcaggt
     8341 cctagagaat ggtacgacga tgaattcgaa gtgtacggac attatatgcc attggatgaa
     8401 ctactagatc aagttgatgt catgatgatg cttcgggtgc aacacgaacg ccatgatgga
     8461 aaagaaagtt tctcaaaaga aggctaccat caggaacatg ggttaacagt tgagcgtgcg
     8521 aagaagttgc aaaaacatgc aatcattatg cacccagcac cagttaaccg tgatgtagaa
     8581 ttagctgatt cattagtgga aggattacag tcgcggatcg ttcagcaaat gagcaatgga
     8641 gtattcgtca gaatggcaat cttagaagca gtgctaaatg gaaaagcgta attataggag
     8701 gatgaaaaaa tgaaaacact cattaaaaac ggtcagatca atacacgaaa aaatatgacg
     8761 acaccagcag aaatttggat agaagatggg cgaatcaaag caatcggtac aggattctca
     8821 gaagccgaat ttgatgaagt gtttgatgca aagggacaat tgatcacacc tggtttagtg
     8881 gacgtccatg ttcatttgag agagccagga tttacataca aggagacgat cgaagctggg
     8941 actcgctcag ctgctcgtgg cggtttcact actgtatgcg cgatgccgaa tttaaatcct
     9001 gtacctgata cggcagaaaa gctgagacaa gtatatgaca tcattagaaa agatgcagtt
     9061 gtgaaggtat tgcagtatgc gccaatcaca gaaaatctgc gaagtgagaa attagttgat
     9121 caagaagcac tgattgaaga aggtgctttt gctttcacaa atgacggtgt aggtgtccaa
     9181 acagctggga cgatgtatct ggctatgaaa gaagctgcaa aaaacaataa agccctagta
     9241 gcccatacag aagatgaatc tttattattt ggcggcgtta tgcacgctgg caagaaggca
     9301 gaagaacttg gtcttcccgg gatcttaagc gtaaccgagt cttctcaaat tgctagagat
     9361 ctgttgctag ctgaagcaac tggtgttcat taccatgttt gccatgtttc tactaaagaa
     9421 agtgtccgtg taatcagaga cgcaaaaaaa gcaggaatcc acgtaacagc agaagtttca
     9481 ccccatcatc ttattttgat cgacgaagat attcccgaag atttcggctt ttggaaaatg
     9541 aacccgccgc taagaggacg ggaggacaga gaagctttga tcgaaggctt gcttgacggt
     9601 acgatcgatt gcatcgctac agaccatgcc ccccatggat tagaagaaaa aagccaaagt
     9661 ttcatgaaat caccatttgg tatcgtagga agcgaaacag cgtttcaact gatctatact
     9721 cattttgtag aaactggtcg attcacgttg gaacaagtga tcaattggtt ggcagttaaa
     9781 ccggctgaga tttttggttt gaatgcagga acgctaacgg ttggtgcccc cgctgatgta
     9841 gcagtttttg atattacaca aacttgcacg atcgataaag aagacttttt atcaaaaggg
     9901 gagaatacac cttttatcgg ctggaaagta aaaggcgaaa cacaaatgac ttttgtcaac
     9961 ggaaaacttg tgtggcagaa gggagaataa aagtatggaa cgtttgctta tattagaaga
    10021 tggaacagtt ttcgaaggaa aggcttttgg tgcagatgct agtgtcttcg gagaaatcgt
    10081 ttttacaaca agtatgactg gttatcaaga aactatcact gatcaaagtt ttaacggtca
    10141 aatcattaca tttacctatc ctatggtggg taattatggg gtaaatagag atgattatga
    10201 atcgatttct ccaacatgca aaggagtagt agtaaaagaa catgcacgtg tagcttcaaa
    10261 ctggcgaaac caaatgacgc tagattcatt tttgaaacaa aaaggaattc ctggaatatc
    10321 aggtatcgat acgcgcgccc ttactcgcaa aatccgtgaa gtcggaacga tgaaagctag
    10381 tatagttgat gctggagatt ccttcgaaca tgcatttgat cagctgaaag caaccgttct
    10441 agcaaccaat caagtgcagc aagtctcaac taacaaacct tatccaagtc caggaacagg
    10501 tcggaaagtc attgtcattg acttcggctt gaaacatagt attttacgag aattgagtaa
    10561 aagaaactgt aacctcacag tacttcccta taacacagat gccgagacga ttttagcgct
    10621 atcaccagac ggagtcatgc tgacgaatgg acctggtgat ccaaaagatg tgccagaagc
    10681 aatcgaaatg atcaaaaaaa tccaaggaaa agtaccgatt tttggtattt gtctcggtca
    10741 ccagttattt gctttagcca acggtgcaga tacctataaa atgaaattcg gacatcgagg
    10801 attaaatcac ccggtacgag aagtagcgac cggaagaatt gattttactt ctcaaaatca
    10861 tggctatgca gtcgatgaag ccacgattga tccagaaaaa ctcatcgtaa ctcatgtaga
    10921 agtaaatgac ggaacagtag aaggagtcaa acacagagat tatcccgctt tcagtgtgca
    10981 gtttcatcca gatgctgccc ctggtccgca tgatgcacta catttatttg atgaatttat
    11041 ggaaatgatg gacgcaggaa aggagcagta aacagatgcc gaaacgtaca gatatcaaga
    11101 aaattatggt gattggatct ggtccgatca tcattggtca agccgcagaa tttgactacg
    11161 caggaacaca agcctgcctt gccttaaaag aagaaggata tgaagtggtt ttagtaaatt
    11221 ctaatcctgc gacgatcatg acggataaag aagtggcaga tcaagtatat atcgaaccga
    11281 tcacacttga atttgtatca aggattcttc gaaaagagcg tccagatgct ctccttccaa
    11341 ctttaggcgg acagacaggc ctcaatatgg caatggagct ggcagcatca ggtattttag
    11401 aagaattgaa catagaactt ctaggaacaa agctttctgc gatcgatcaa gcagaagatc
    11461 gtgatctatt caaacagttg atgaaagaat tgaatcagcc aatccccgag tctgaaatcg
    11521 tcaatacagt agaagaagct gtagattttg caaataaaat cggctatcct gtcatcgttc
    11581 gtccagcctt tactttaggc ggaactggtg gaggtatgtg tgaaaatgaa gaagaattac
    11641 gtatcgtatc agaaaatgga ttaagtcttt cccccgctac ccaatgtttg attgaacgaa
    11701 gtattgcagg ttttaaagaa attgaatatg aagtgatgag agattcggct gataatgcaa
    11761 tcgttgtctg caatatggaa aattttgacc cggtgggtat ccatacaggt gattctatcg
    11821 tatttgcccc tagtcaaaca ttgtctgacc atgaatatca aatgcttcgt gatgcttctc
    11881 tttcgattat tcgagcactt aagatcgagg gaggctgtaa tgtccaactc gctcttgatc
    11941 cacatagttt taattattat gtgattgaag tcaatccgcg tgtttctcgt tcatctgcgt
    12001 tagcaagtaa ggcgactggc tatccgatcg ctaagctagc tgctaaaata gcgcttggat
    12061 taacattaga cgaaatgaaa aatcctgtta caggaacgac ttttgcagaa tttgaacctg
    12121 cattagatta tgttgtagct aaaattcctc gctggccgtt tgataagttt gaaaacggag
    12181 aacggctttt aggcacgcag atgaaagcaa ctggtgaagt gatggctatc ggtcggaata
    12241 tcgaagagtc tttattgaaa gctgtgcgtt ctttagaaat cggtacacac catatcgaac
    12301 ttcctgagtt ggcagacatc acggacgatg acctaatcca aaaagtggtt cgagcacaag
    12361 atgatcgcct gttttatgta gctgaagcta ttagaagagg gtacaccata gaagaattag
    12421 cggatttgac aaaaatcgat ttattctttt tagataaact gttgcatatc gtagaaatcg
    12481 aacagcagtt gaaacagcag ccgttggatg acaagatatt gaaaacagcg aaacaaaacg
    12541 gttttacaga caaaaaagtg gcggagctgt gggaaataaa cgaagaagcc gttcgccaga
    12601 tgagaaaaga aaatgatttg attcctgtat ataagatggt tgacacttgt gcagcggaat
    12661 tcgaatcaaa aacaccttat ttttatagtt cctatgaata tgaaaatgaa agttcacgat
    12721 cagaaaagcc ctcaattcta gtactgggat ctggtcctat ccgtatcgga caaggggttg
    12781 aatttgacta tgcgactgtg cattctgtca aagcaatcca gcaggcagga tacgaagcaa
    12841 tcatcatgaa cagcaatcct gaaacagttt cgactgactt ttctatttcg gataaattat
    12901 attttgagcc attgaccttc gaagatgtga tgaatgtgat cgagctagaa cagccgatag
    12961 gagtaatcgt tcaatttggt ggtcagacag caatcaactt agccgaacct ttgactgctg
    13021 ctggtgtaaa aatcttaggg acaacgatcg aagacttgga tcgtgcggaa aatcgtgatt
    13081 tattcgagca agcgctgaaa gagttggata ttcctcaacc tttaggggat actgctacaa
    13141 gtaaagaaga agccgtggtt atcgcttcga gaattggtta tccagttctt gttcgtccaa
    13201 gctatgtatt aggcggacgt gcgatggaaa tcgttgaaaa ccagcgagat ttagaagatt
    13261 acatggaaca tgctgtgaaa gcatctccag aacatccagt actagtggac cgttacttgc
    13321 ttggtggtga atgcgaagtg gatgcgattt gtgacggtga aacagtactc atcccaggga
    13381 tcatggagca tattgagcgt gctggtgtcc attcagggga ttcaatggct gtgtatccac
    13441 ctcaaacatt atctgaatcg atcaagaaaa cgatcgaaga ttacacgatc cgcttagcat
    13501 taggtttgaa ttgtgtcggg atgatgaata tccagtttgt catccatgaa aatgaggttt
    13561 atgtgatcga agtcaatcct cgagcaagtc ggactgttcc attcttaagt aaggtgacag
    13621 gaattccaat ggcacaaatt gcgacgaaag ctattttggg agagaaacta aagacacttg
    13681 gttatgaaaa cggactttat ccagaaacaa aagaagttca tgttaaagca cctgtctttt
    13741 cttttacgaa attgcagaaa gttgatactt acttagggcc tgaaatgaaa tcgactggag
    13801 aagtaatggg atctgacaga caattagaca aagctttgta caaggcattc gaagcttctg
    13861 gattacactt gcctgaatat ggagccgtat tatttacgat tgcggacgaa acaaaagaag
    13921 aagcactaga attagctaaa cgttttacag aaattggcta tagtctgata ggaacgaaac
    13981 atacagctgc ttatttcgaa aaaaatggtc tggtcgttga aacagtagcg aaaatttctg
    14041 aggaagcagc agaaaaaaat gtagtcgact taataagaga tggtaaaaca caagtggttg
    14101 tcaatacgat tgataaagat cggggaaatg cttctaaaga cggtttcatc atccgaagag
    14161 aagcagttga acatggtatt ccattgttta cttctttaga cacagcagat gcgatcgttc
    14221 gagtgatgga atcccgtgct ttttcaacta accctattta acggcgagca catgttaaga
    14281 caaaaagacg aaaaaccgtt gcaggagggg acagatgaaa caagaaatga tggaaatcgt
    14341 tcagcaaaaa cagctggcac caaagatttt tgaaatgaca ttgaaaggtg atctagtaaa
    14401 agaaatgcag caaccaggac agttcttgca tattctcgtg ccgcgtgcag acttgctatt
    14461 acgccgaccg attagcttga atcatatcga ccacgaaaca aatacttgcc gtatcattta
    14521 tcgggtggaa ggtgagggga ctagagtctt ctctactctt tctgccggag accatttaga
    14581 tgtcatgggt cctttaggca atggttttga tttgtccaat cttcatacag gagacgaagt
    14641 atttataatc ggtggaggaa tcggtgtccc tcctctatat gagttatcaa aacagttagt
    14701 cgaaaaagga attaagccaa cgcatttttt aggttttgcc acacatgaag tcgtttatta
    14761 tgaaaaagaa tttaatcgtc taggagaaac aagaatcgct actgacgatg gaacatacgg
    14821 tatgcacgga aatgtcggta atttattgtt aggaacgaag acggaaccta cagctgtttt
    14881 tgcatgtggt tcaaacgggt tattgaagac agtggaacag ctgttttctt cgcatcaaaa
    14941 tgtccagtta tcattagaat cccgcatggc atgtgggata ggggcttgct atgcttgcgt
    15001 ttgtcatacg ccggaagatg aaacaggaac aaaaagtgtg aaagtttgtg acgaaggacc
    15061 gatttttaaa ataggagagg taattatatg aatgagaatc ctttagcggt caaacttcct
    15121 ggcctagaat taaaaaatcc tatcatgccg gcaagcggct gtttcggttt tggcaaagaa
    15181 tacggaaaat actatgattt gaatcagctt ggatcgatca tggtcaaggc aacgactcca
    15241 aatgcccgct ttggcaatcc cacgccaaga gtggcggaaa caccatctgg catgttgaat
    15301 gcaattggtt tgcaaaatcc tggtctagac gttgttatgc ataaacttct tccagaatta
    15361 gaggagtaca atgagttacc catcatcgca aatgttgctg gtgcttgtga agaagactat
    15421 gtagaagttt gtagcaaaat tggcgaagca ccaaatgtga aagcaatcga gctgaacatt
    15481 tcttgcccaa atgtcaaaca tggcggaatt gcttttggaa cagattctga tgttgctttt
    15541 caattaacac aagcagtaaa gaaagtttct tctgttcctg tctatgtcaa attgtcccca
    15601 aatgtaacag acatcgttcc aatcgctcag gcaatcgaag ctggaggggc agatgggttt
    15661 acgatgatca atactcttct tggtatgcgg attgacctta agacaagaaa accgatttta
    15721 gccaatcaaa caggtggact ctccggacca gcaatcaagc ctgtcgctat tcgtttgatt
    15781 caccaagtag caagtatatc cagccttcca atcattggga tgggcggcgt gcagacagtg
    15841 gatgatgttc ttgagatgtt tatggctgga gcaagtgctg tagctatagg gaccgcgaat
    15901 ttcaccgatc cgtatatctg tcctaaacta attaaagagc tccctgtgcg aatgagcgaa
    15961 ctaggcatcg aatcgctgga aagattaatc aaagaagtga gagaggaacg tgaacgatga
    16021 atcaactaga ccaacgacca attattgcat tagatttttc aactaggcaa gaggtagaag
    16081 actttcttcg atttttcccg aaagaagaaa aattgtttgt gaaaatcgga atggaattgt
    16141 tctaccaaga aggacctgaa atcgtacgtt atctaaaaga ggctggtcat aatgtatttt
    16201 tagatctgaa acttcacgat attccgaata cggtcgaaaa agcaatgcgg ggattggcaa
    16261 agcttggtgt tgatctcacc tgtgttcatg ccgctggcgg tattcgtatg atggaggcgg
    16321 caatgcgagg attagaagag gggactccag aaggtggaaa acgtccgcta cttctagcta
    16381 tcactcagct tacttctaca agtgaagaag aaatgcatgc cgatcaacta atcgaagttt
    16441 ctttagaaaa aagtgtgatc cattatgcta gctgtgccaa aaaagcagga ttagatggtg
    16501 tggtttcttc tgcttgggaa gtagaagcga tcaaagaaat ggcaggagac gaatttgttt
    16561 gcttaactcc gggtataaga ccagaaggaa cagtcgcagg agatcagaca agagtagtca
    16621 caccttctca ggcaaaaaag atcggctcaa cttttatcgt tataggtcga ccaatcactc
    16681 agtcaactaa tccttataaa gcatatcaaa cgattcaaac agaatggagc taacctaaaa
    16741 tgaatgttga acaaagtatc gcaaaagact tattagaaat tgaagcagta tttttaaatc
    16801 caagtaatcc ttttacatgg gcaagtggaa tcaaaagccc aatttactgt gataaccgaa
    16861 tcacgatgag ctatccaaaa gtccgaaaag agatcgcaaa aggattagca agcaaaatca
    16921 aagaagctta ccctgaagta caagtgattg ctggaactgc aacagcagga atccctcatg
    16981 ccgcatgggt agcggaaata ctggatttgc caatggtcta tattcgaagt aaggcgaaag
    17041 atcatgggaa aggtaaccaa atcgaaggac gtatatttga aggtcagaag atggttgtga
    17101 tcgaggattt gatttctaca ggcggaagtg tcttggaagc agctgaagca gcaaaaagag
    17161 aaggtgcgga tatccttggt gttgcggcta tctttacgta tgagttgcca aaaggaaaag
    17221 caaatttcga aaaagccgaa atccctctta tgacgttgac caattattct gtattgattg
    17281 aagcagcttt agaagatcgt tatatcgatg agcaagaatt aactctgcta aaagaatgga
    17341 aaaaagatcc tgaaaattgg cagatttaag cgcgcttgat gaagatagag aaatccgatt
    17401 tgtgtctcca gaatcatcaa ttggaatagc aaaaatccaa aaactgattg cgagaagcat
    17461 cttttcatca ggtatgaaaa gaaacttcta tcgtgatcag tttttgattt ttttaaaatt
    17521 catttttcta agactggtta gcagaaagaa gagaataatc tcttctcttt tttctattat
    17581 atatagattt tctctatttt ttggtaaggg agctgtttca aaaatggtaa agagcggaaa
    17641 tgttgcgaat atcagttatt taatgatatg atattcggaa aaagaaatct caaagggagg
    17701 taacttttat ggggttgaat gaatttattt taggattgaa caatttagag acgatcacaa
    17761 gggcacctgg attttttaaa tatacagagc atacagttgc agcacattcg tatcgtgtag
    17821 catctattgc gcaagttcta ggagacattg aagaagcgaa tggacaaccg atcgattgga
    17881 aactgctcta tgaaaaatca ttgaatcatg actacacaga gcgttttatt ggtgatatca
    17941 aaacgccggt gaaatacgca agcaaagagt taagagggat gctgcaaaca gttgaagaaa
    18001 agatgacgga cgaattcatc aaccaagaga ttccagctga gttccaagaa gtttatcgcc
    18061 gtcggttgtt cgaaggaaaa gatgaaaccg tagaaggaga actattagcc atttcagata
    18121 aggttgacct tctttatgaa tcttttgatg aaatcatcaa aaataacccg gaaaaagttt
    18181 ataaggagat gttcattgag gcagtgacta cgattaaaag ctttgaacat cggccttctg
    18241 ttcagtattt ctttaaagag atatttccgg aactattgaa ccaggaattt tatggaaaag
    18301 aggcgttttt agaaaccgtc tcaacgattg taaagaaaga agaaaaataa aaatgatatg
    18361 agatgcaggg ggaagtatag ttgaaaaaga atatggatgt cgaatggggc tattctggag
    18421 acaaaggtcc ggaaaattgg gcgagtttat gtgattggtt tgcgagaggt gcggaatttc
    18481 cattgcagtc gcctattcat ttgaccaagg gggaagaaac caaaatagaa ggagacacgc
    18541 tttcctttca ttatcaaaag caacggttta ctgacaagga attcaaaaac acgattcatt
    18601 tagtaccttt tgaccaactc agttatgtag agttcaagaa agaacgctat tatttgacag
    18661 atattcattt ccacttgcct tctgaacata tcatcaacgg aaaacaggaa gatattgaat
    18721 ttcattttgt tcatatgaac agcaaaaaag agaatctagt tgtgggtgtc atgtaccagc
    18781 taatggtaca ggaaggttgg ctgtataatc aagaaaatgg ggaaagttgg aacttagaaa
    18841 aacatgagca ttgggtaaat ccggatgtgt tctttccgga gcaaaaaagc catttccatt
    18901 atattggctc attgacaact ccgcctacca gcgggccaat ccaatggttt gtaatggatc
    18961 atgtaggaaa actgaaccaa gagtttcttc tccccttcga tggtcagtat gctcgaccga
    19021 acaaccgacc aatccagccc ttgaatggaa gagaaattta ttatttcgag gagtttgagc
    19081 aatgaatatt cagcagatga aatacgtggt cgctgtagca aacaatggta gtttcagaga
    19141 agcggcaaag aaaatgtttg tctcccagcc aagtctttca gctggtatca aagaattaga
    19201 aaccgagtta gggatcacgc tgtttactcg aacgaatcga ggggcatatt taacagaaga
    19261 agggcaagac ttcttaaatc gtgccgagaa aatattggtc cagcttgata cattagaaac
    19321 tcactatcta acgagtgaaa agagagacaa tttttcgatc gctgcccagc attatgattt
    19381 cttaggtcct ttgaccgcac agctactcga ggaattcaaa gacgagatca aacaatttcg
    19441 gatcatggag acgacaacag cgagagtgat cgaagaagtc aaagagcagc acagtgagat
    19501 cggcattatt tatatgaatg agcataatca agctggtatc agaagatatt tacaacaagg
    19561 agagctaagt ggccgcagca ttggaaaatt caaaacgcat atttttttaa ggcaaggaca
    19621 tccattagct gaacacgcag agatcaccaa agaagaattg ttatcttatc ctcaagttcg
    19681 ttttacacag gatggaaata atttcccgta tttttatgaa gaccttatcg agatccctga
    19741 tcaagaaaca gtgatttata cgagtgatcg aggtactttg atgaatatcg ttcttgaaac
    19801 tgatgcttac gcttcaggat ctggtattgt gattggaggg attcgcgagc acttgcggtt
    19861 aatccctttg gcagaaggcg aactaaatga attttatatc atttattcgg cgaaacgtcc
    19921 gctcagtgaa atcgctcaaa gatttatcaa agagcttact cggttactgg accagcagag
    19981 ctaagaaaag cagatgagaa cgctgtttat tgaacaccgt tgatcaaaaa agaggggttg
    20041 tgacagaatt ttgtcacaac tcctcttttt gtagcaaaca atttataact aattgctgtt
    20101 gtgggaaagt acgcaaatat gattctagaa acactcattg attacctgtt ttgtttcata
    20161 gctgtaattt tttcttcttc tggtgtcacg ataatcgtct tttggttttc ataaataaca
    20221 taaccaggct tggcgccatt tggttttcgg atatgcttta cctgtacaag atcaaccggc
    20281 acttgggcag agtatcggta ttttgaaaaa taggcagcta actcggcagc ttctgtaatt
    20341 gtttcatctg agggcttgtc acttttaatg atgacgtgcg aaccgggtat attttttgca
    20401 tgaagccaat agtcggtttt tttagctgtc ttcattgtca gctggtcatt ttgaagattg
    20461 tttttaccaa caagaatgag cgttccgtca gaagaaaagt attgatcagg ctgagatggt
    20521 ttcttacgtt tttgtttttt taagttcttc tttttcaggt atccttctga agtcaattct
    20581 tctttaattg cttcgatatc cattgggccg gcaatttcca gttgagataa gacagattca
    20641 agatattgga tttcatcttt ggcttcttgg atttgttcgc caatcagctt aacagcgttc
    20701 ttcagctttt ggtatcgatg gaaatatttc tgtgcatttt gatttggtgt caatgccgga
    20761 tctagtgcta ttttgattgg ccgatcttct tcataataat taggtaaaac aacttctttt
    20821 gcgccgcgtg gtacttgtgt cagaaaagta gtaagtagtt cgccatttct acggtaattc
    20881 tcagcgtttt cggattcttt gagtgtttgt tcacgttttt tcaatttatt tttatttcgt
    20941 tttagttcat tttcgatttt tcgaatcagt tctccgcctt gttgttttgc ccgatctttc
    21001 tctgcttttt cgtgataata gctatctaat aattgactga gtgttggata agctgttaca
    21061 gcagatgcct gttcggacaa tacctgataa gcaatcggtg tgaaaaaatc tttattttcc
    21121 gtttcgtaaa aagtaggaat aggctgatgg ctgatggctg agaaaaattc gtcccataca
    21181 accatctttt cattaggacg ttccagtaat cttttggtca gctcttgagc gatatcgaaa
    21241 ccgattccct gaaactgacg ttggattgct tttggatcta gttcagtttg ggaaagccgc
    21301 tgaaagattt tttctttctc ggagctaaat gggttcaaga cctcttgttt tggcggagca
    21361 acatattcta caccaggaag caaagaacga taagtgtttt gagagcttcc aatgtgtttg
    21421 atcgcatcca gtatttttcc agtttctcga ttaactaaga cgatcgtact atgacgtccc
    21481 attaattcaa cgatcaatat gatattttgt agatcaccca actcgtttcg tttagcaaag
    21541 tggaaatgga tcactcgatc attttcgatt tgttcgatgt cttctaaaat agcgccatcc
    21601 aaatattttc gaagcatcat cacgaagtta ggcggattat caggattctg ataatcgatt
    21661 tgggtaatct gtacacgagc ataacttggg tgcgcagata ataataatcg atggtttttg
    21721 ccgcgtgaac gaatcaccag cacgatttca ttttcataag gctgatggat tttcgagatc
    21781 cggccagata gtaacgtttc acgcaattcg ttgaccatcg cgtgggtaaa tacaccatca
    21841 aaggacatgg ttcattcctc atttcttttc ataagtatat tcattataac gaaaaaaaga
    21901 agaaaaacca atttggccga atagtaaagc aaaagtctag aaaaatcttg gactaattac
    21961 ataaaaaaca gtgtttctat cgttgaaatc caaattttta ggtataataa aaaataggag
    22021 aaaaagtaga aaagaaggtg tttgttattt gggaagtagt taaatcgaga gtacaagaaa
    22081 tgaatgaaca tttctcagtg tcattcgcag aaaatccaag taagtatttt cgacgtcttg
    22141 tttttctgct catcttatca ttgctgttaa cagttgcaat gtttttattg ccggaaaaaa
    22201 tccgagaaga gcaagtaaat agtctcgttt attcgtccat accaaccagc aaattgaaac
    22261 cttttgatta cagcaaagct gatcagaaaa tcaaacaaag ccgggcgata tcggtgatgt
    22321 tcagcaaacc gaacgggaaa acctatcagg acatgcttga tgtttttaat gatcctgagc
    22381 ttatggaaga attgaatcga ccgatctttt attatccgat cgtttatgat gtacatgaac
    22441 tggaacaaaa atataacata cgaacggatg aagtgacatt tattttcttt gatggaggaa
    22501 aagaagcaaa tcggataact gcgggtaagg gaggaataac tgattttgat aaggagttga
    22561 tccctgaact gaatcgactt ccgttagcca atatcaaaca attagaagaa gaattggata
    22621 gaacgggagt aacttcccaa aattccgaga ttgctaattg agacattggc gcgatgaaac
    22681 ggtgggtcta tatagcgcta tttttaatta tcagaacaag tgatgtttga tcttcaaaat
    22741 aacaaataaa aaattaaaaa atatagttga cgaaattcaa aaattcgatt agacttaaat
    22801 caattcttaa ggaaagggtg acctgtaatg aacttatcaa aacgtatttc agcagaaaac
    22861 aaaaaaaatt ggcgcagctg gcgtagataa gttgtattaa tggtgagact ttcccataga
    22921 tacaactttt tagagtgcat ttctaaagct tgtatctatg ctagacatga acattgactg
    22981 ccgcatagat tagaatctat gcggtttttt gtgtaaagac aatgaccgct ttggccattg
    23041 tctttttttg tagactggat acttgcctga ttataataaa taaaaagatt gactttgcag
    23101 gaggaaaaaa atgaaaaaca aacgattaat aacagttgta gtaattattt tagtttattt
    23161 agcaggtgca tttatttatg gaaaagcctc aggcgaaaaa gaaagtactt cgacgaacca
    23221 gacaaaaaaa acaacagttg ttggggtatt gcaatacgtg agccatccgg cattagatga
    23281 gatttatcga ggaatcaaag atggtctgaa agaagaagga ttaaaagaag gagaaaatct
    23341 tgagatcaaa tttcaaaatg gacaagccga tcagagcaag ctagccacaa tgagtcaaca
    23401 gctagtacaa tctgatccag atgccttgat tggaatcgca acacctgccg ctcaatcatt
    23461 agcaaacacg acttcatcga tcccagttgt gttaggtgct gtaacggatc ctgtgggggc
    23521 tggattagtc gcttctttag agaaacctgg tggtaatgta acaggtgttt ctgatcagcc
    23581 gcctgttact tcacaaatca agcttgcttc tgatttgttg ccaaatgcaa aaaaagtcgg
    23641 aatcttgtat gcttcttcag aggataattc aaaataccaa gtagcacaag cagaagaagc
    23701 tgcaaaaaaa gaagggttag aatcagtaaa atatgcggtg ccttccacta atgagattgc
    23761 acaaacggtt caagtgatga gccgacaagt cgattttatc tatgttccat tagataatac
    23821 cattgccaat gcgatgcaaa cagtggtaaa agaagcaaac aaagcaaata tccccgtcat
    23881 tccatctgtg gatacaatgg tggaacaagg tggactagca acaatcggga tcaatcaata
    23941 tcaattaggg cttcaaagcg gtaagatggc ggcagaactt gcgagtggaa aagaaaaacc
    24001 ggaaacgacg cctgtttata catttgatca aggggataca gtgatcaatc aatctcaagc
    24061 tgatcgctta ggaatcattg ttccccaatc gatgaaggaa aaagccaaaa tcattacaga
    24121 tgactcgcag caagaaacat ctaaggagga tgacaaatga tcgtatcagc tatttcacaa
    24181 ggcatgcttt gggcaatatt aggattaggg attttcatga cttatcggat cttagacttt
    24241 cctgatatga cgacagaagg gtccttccca ttaggtgctg ctgtctgtgt gacagctatt
    24301 actcatgggg tagcgccgat tgtagctaca ttattaggcg taggtgctgg catgatagca
    24361 ggactcgtta cggggttact ttatacaaaa ggaaagatac cggttatttt agcagggatt
    24421 ttagtgatgt caggattgaa ctcagttatt ttgtttgtga tgcgaacacc aaatctttct
    24481 ttattgaatc atccgattct acaagacagc ttccatcgct ttgggttacc tgattacttt
    24541 gacacagtcc ttcttggctt catctctttg ggcatcttga ttgctgtact gctattcttc
    24601 ttcaatacag atttgggtca aggctatatt gcgacaggag acaacgaaac gatggctcgg
    24661 tcgttaggga taaaaaccga tcgtatgaaa attttaggtt taactttgtc gaacggtgtc
    24721 attgcattat ccggtgcgct gattgcccag aacgacggat atgcagatgt aaataaaggg
    24781 attggcgtaa tcgtcatcgg tcttgcttcc atcatcatcg gtgaagtgat cttccaagaa
    24841 ttgacgctag cagaaagatt gattgctatt gttgtcggca gtatcattta tcagctattg
    24901 attttagtag taatcaaact agggtttgac acgacttatc tgaagttgtt ctccgctatc
    24961 atcttagcta tttgtctgat gatcccgcaa ttgaagaaag cactgaactt aaaaacgggt
    25021 tttgagaagg aggcatagta atgactgata tattgacaat caaaaatggc agaaaagtcg
    25081 taaacaatgg aatgaatgaa gagaaagtac tattggatga aattaacctt acaatcagag
    25141 aaggggactt catcactgtc cttggcggga atggtgctgg aaaaagcaca ctgttcaata
    25201 cgatcgctgg tacgcttcaa ctcagtcaag gatcgatctc ttttaaagat cagctgatca
    25261 caaaagattc agaagaaaaa agagcacgat tcctttctcg agttttccaa gaccccaaga
    25321 tgggaacggc tcctcggatg acagtagcag aaaatttgct acttgcacaa aaaagaggtc
    25381 aaaaacggaa attccgttta cgtaacttga aaaatcaaaa agaagatttt ttcagactat
    25441 gtcaagaagt cggtaatggc ctagagcaac acttggatac tccagcgggt gaattatctg
    25501 gtggtcaacg ccaagcatta agcttgctga tggcgacgat cacagctcct gagttactat
    25561 tattagatga acatactgca gctttagatc ctaaaacagc taaagcatta atgcgattaa
    25621 caaatcagcg gatcaatgaa caaaaattga cttgtctcat gatcacgcat cgaatggaag
    25681 acgccttgaa atacggaaat cgtctgatcg tactccaaaa agggaaaatc gttcaagata
    25741 tttctgaaaa agaaaagcaa catttgacaa tgggcgacct tttgcagttc tttgaagaag
    25801 tagtggattg atttcgacag aaaaattcct ttcagtagaa ggacttgaaa actcattgac
    25861 atcttccttt gattcatcct atacttaaaa caataaacaa ttaaacatat ttgggtgttt
    25921 tgtaggatca cgtagtcaat gattttaaca attaaataga atattctttg ctctgacaag
    25981 taaacgatga gaaaagaatt tgctactttg ccacctagac gtttcgtact ttttcgtatc
    26041 aaacgctaag tggctttttt tattgaagta agaacaaagg aggaataaga tgatttctgg
    26101 acaaaccaaa ttagctgggc tctttgctga tccggcttct cacagtttat ccccattgat
    26161 gcacaatact gcttttgaag caaacaacat cgatgcggtg tatttagctt tccaagtaaa
    26221 tccaatgaat ttgaagcaag cagtcgaaag tattcgaact tttgatatgt taggtgtaaa
    26281 tctttcgatg ccgaataaaa cagcggttat tccttatctg gatgaggtaa gccaggaagc
    26341 gcaattgatc ggagcagtga atacgatcgt gcacgaaaag ggtcgtttga ttggctataa
    26401 tactgatggt gcgggcttca tgcaatccgt gcaagaagct gggatttcga tcaaaaagaa
    26461 aaaaatgact ctactgggag ctggaggtgc agcaaaggca gtcactgttc aagcagcgtt
    26521 agacggcgct gaggaaatcg tagtttataa acgaaacaac caaacattcg ctcaagtaaa
    26581 agaaacattc gaacaactca gctatcgaac ttcttgtaag ataaaagttc gtgactttgc
    26641 cgatacgcat caattaagaa aagacttgaa agaaagctgt cttctagtca atgcaacaga
    26701 tatcgggatg ggaaagaaaa tcaatcagtc accgctctta gatataagca tgatgcatga
    26761 gaatctagcg gtttttgatt tgatttatgc acctagagaa acacgtttat taaaagaagc
    26821 aaaaaaagta ggagcaaaag gatacaatgg acttggcatg ttgatctacc aaggagcaat
    26881 cgcgtttgaa ttatggacaa aggaaaagat gccgatccat gtgattgaaa agctgtttga
    26941 ttaaaaaagg ataatagata aagatctttt attctcgcat aaacggtctc aagaaaagta
    27001 atcctttatg tatcgaagca tttatttcgt tattttttat taaattatca aaaaatgata
    27061 ggcttttgtc gattggttat cgtatccttt atttatcagt aagaaacgag gaggaaccac
    27121 aatgagtcgg atggatcgct ataaggacat ccataagaag gcgaaacctc tgaagcggga
    27181 tacggggagt atttttgcta gaagagaacg aaaacgaacg cgtatggaaa atgaggaaga
    27241 tgctacccgt atttatgaaa gagagcagca ttcatttgaa catttgaacc aatacgagca
    27301 acagatatac agagatgcac caagagcaga agaacaacaa aaaaataaaa aatttttcaa
    27361 tcggcaaaag aagaacaaac cgttaaaacc tcgaagaaag cgatcgtgga aaaaaatcat
    27421 tctatcgatt ttgcttgttt tgatccttta ttcaggtatc tcatttttta ttggtaaata
    27481 tgtagctgaa catgacacaa gcttagaggc agcgacttca gaaacctttc atgggcaaaa
    27541 agcgacgaat ggtgcgcata atattttgat ccttggaagt gatacacgcg gagaagatgc
    27601 cgggcgagcc gatacgatca tggtcttaca gttggatggt ccttcccata agccgaaact
    27661 gatttctttt atgcgtgaca gcttcgttac gatcccaggg gtaggacaaa ataagatcaa
    27721 ttcagcttat gcatacggag gagccgatct agtgcgtcaa actttagtag aaaattttgg
    27781 aatcgattgt caatactatg cgaaagtcga tttcaaatcg tttgaaaaag tgatcgatgc
    27841 cttattcatg aacggagtga agatcgatgc agaaaaagat ctgaatctgg atggtgtaga
    27901 tattaaaaaa gggggccaga agatggatgg acatgttctt ttgcagtacg cacgtttccg
    27961 aatggatgaa caaggagact ttggccgtgt cagacgccaa cagcaagtaa tgaacgctat
    28021 tttcagccaa ttgaagaatc cattgaatct gatccgtgca ccttatgcag ctggtaaagc
    28081 tttaggatat acatccacag atgtcactgc ttggttcttg acgaaaaacc tcttttcgat
    28141 tgcacgagga gctggtggca ttgatcgaat aagtgtgcca gtagacggtt cgtggaatta
    28201 tgggcaaagt gattatgctg gcagtattct tgaaattgat aatcaaatga atcaatctgc
    28261 tattacagat tttttgaata aatgatcata aaaaagatca agaagaagct gcgcaaggct
    28321 tttgtcatga atcaatcaaa cgattcactc gtaaaaaacg ctttcttatg gtagatttaa
    28381 ccaagagagg agctgtttac attggatatc tatctgaact tcaaaaatga gtcgcgtgaa
    28441 gccatcgctt tttatgaaaa agtttttcag actgaagcat caggtatcac cacatacggt
    28501 gaaatgcctg gaatagaaca aatgaacttg ccagaagaag caaaaaatct aatcacgaat
    28561 gcacggctga acatttatga tacattagtt atgtttgcag atacgccaga aggaattggt
    28621 cctgaattag ttgtgggaaa taatgtttct ttagtcatcc agacaagaaa tgaagaagaa
    28681 acgaagcgcc tatttgaaag cttagctgag ggtggtaaga ttgagatgcc actggatgct
    28741 gttccgtggg ctcagttgta tgggatggta actgatcgat ttggcatcaa ctggcagatt
    28801 aaccaagagt gacagctaaa gatgaatatt ttctggaaac atcaagcagc aaacggatca
    28861 tcgtttgttt gcttgatgtt tttgtgtcta tttcgactat acggtatctt ttttttattg
    28921 ccagatgcga aaatatgttc cataatagag aagtccgcaa aaaattaagt aacggaggtc
    28981 agaagatgaa gacggtgata ctagctgaaa aaccttcaca agcaaaagcc tatgccgaaa
    29041 ccttccaaca gcaaaagcgg catgaagggt attatgaaat cagcgatcca ttgtttgctg
    29101 gtgaagtaac catcacttat ggttttggac atttggtaga tatggttgct ccaggagctt
    29161 atgaagagcg ttgggcaaaa tggtctttag aaaatttgcc tattttccca gaaaccttcc
    29221 gttacgaagt tccaatggac aaacgtgcac aattttcgat cgtaaaacga gaattgcagt
    29281 cagccgatac catcattatt gcaacagatg gtgatcgaga aggtgaagcc atcgcgtggt
    29341 cgattattcg acaagcaaac gcattttcta aggaaaagaa ctatttacgt ttatggatca
    29401 attctcttga aaaagaagca atctatgaag gttttaagaa tctccaacct ggaaagaact
    29461 attttccaaa atataaagaa gcgcaagcaa ggcagaacgc tgattggctg attggaatga
    29521 atggaagtcc attatatagc ttgctacttc aacgaaaagg gattgatggc agtttttctc
    29581 ttggtcgtgt acaaacaccg acactctata tgatttatca gctgcaagaa gaaatcaaac
    29641 acttcaaaaa agaaacgtac tttgaaggag aaggtttaat tacgacaaac aaagggcagt
    29701 tttctggaaa aatcgtacca aaaaaagcat ttaaaaccca agaagagtta acgaaggaaa
    29761 tagaaaaact tggagcgcat ttaggtaagc aaaacggacg gattcttgaa gttacgaaaa
    29821 aggaaaagag gttgcatagt ccacgtttat tttcgctatc tagtttgcag acaaagatga
    29881 atcagctaat gaaagcaagt gccaaagcta cacttgatgc tgctcaagga ttgtacgaaa
    29941 gcaagtttct aagctatcca agaacggatt ccttttacat tacagaaaat gagcatcagt
    30001 atctggttaa acatctgcaa aattataaaa aatttttagg aatagaagaa gtacagacaa
    30061 ctgagatcaa agcaaagaaa cgatatgtcg atgcgaaaaa agttcaagaa catcatgcaa
    30121 ttattccgac aaagacgatt ccgactcctg cacgctttgc gaagctttcc aaattacagc
    30181 aagcaatcta tttgcaagtt ttgcgcacta ctgtggctat gtttgctgca gattatatct
    30241 acgaagagac gaccgtaatg acgggcgtac agcaattaaa gctaaaaagc atcggaaaga
    30301 tcccattaca gcaaggttgg caggaaatct tacgcatcaa gtcaaaaaag aaagaagctc
    30361 agactcttcc tcctatcgta gaaaatgaag aagcagaagt agaactgaaa agtatggaga
    30421 aagaaacaca acctccaaaa ccctataccg aaggaacgtt gatcacagca atgaaaactg
    30481 cgggaaaaac gctggataat gatgaagcgc aagaaattct caaagaagta gaaggaatag
    30541 ggacagaagc tacacgagcc aatatcattg aaaatcttaa gcagaagcat tacatcgaag
    30601 tcaataaaaa cgaaataact gttacgccaa aaggaatcac tctttgtaag gcagtagcca
    30661 atgaaccatt actgacgagc gcagaaatga ctgcgcgctg ggaagggtac ttgagaaaaa
    30721 ttggtaaaca agaggggaca ccatctgttt ttttagagaa tatcaaaaaa ttcatcctac
    30781 atttattggc agaagttcct aatcaaatcg acaaggtaga cttttcagat gagattagcg
    30841 ggacacgtca gataaaagcg atggagaaga aaaacgatca attaggaaga tgtccaaaat
    30901 gtaaaaaggg aatagtcatg ctttatccta aaatcgctac atgcttgaat ccggaatgcg
    30961 acttcaaatt gtggccaaca gtagctaaaa agaagctgac taaaacgaat ctcagagatt
    31021 tgctttcaaa aggaaagaca agtaaagttg tcaaaggttt tactggtaaa aaagggaaat
    31081 tcgatgcagt tttagtatta aaagaagata tgacaatcgg tttttctttt ccatggatcg
    31141 aaaacagtgg aacaaaagca gttgatggaa acaaagaaac atcaagaaaa aataatgaaa
    31201 acgaagtaga gtaaatgaaa acaatgaaat agcggaatat ttattccgaa aagtgagaat
    31261 atagcaatca cgattgtggc tgtgtaccaa gcatttctaa agtatcctga ttacgggacg
    31321 aagttgttgt cattgacgca caatcaaaca gcatattcac caacgttgat tttatcgact
    31381 ttgactgcag gagctaaaag cgctacttat ttttttcata aaacgacaag catgctaaag
    31441 cgaaagaaac agcgaaaaca attgttggtg gtggtagcgg ctatgatcat tatttatttt
    31501 ctaccagtta ttacacaagt atttcatcaa tattttggtt aagaatacga gagtttgtga
    31561 caaaagtcat gtcacaaact tttttctttg tcattttttc gtaagaaaaa gcaaaataaa
    31621 agtatataca ttgattaaaa gtatatactt ggatataatg tacttactat agaatgagaa
    31681 tggtatagag ggtggcgaaa tatgaaaaaa atgaaaaagt ataaaattag aaaatctggc
    31741 aatagcgatg taacaactat cccaccagaa gtaaaagaat tcatgggtgt gcagacaggt
    31801 gacgcaatca gttatgtttt tcaatctgat ggttctgtac gaatgatcaa agcacaagaa
    31861 gaacctgata ttgattcatt agttgattcg atcatgaatc aatatgaaga cgcgttaaaa
    31921 gatttggttg atttatgagg tatctttcga ctaaagaaat tatcaaaatc aacgcgaaag
    31981 ttattttacc gtcactcgcc aggcgaaatg atcggtgtaa aagatgcaaa tgcattagat
    32041 atggccgtca aacagccttc acaagcagta ttcaatcaag aactctatcc tgaaatctat
    32101 gaaaaagcgg cgattttagc aattaattta gctaaaaaac atccttttca taatggcaat
    32161 aaacgaactg ctcttgttgc catgttgttg tttcttcaat tgaataattg tccagttgct
    32221 ttttctcgtc aagaagctgt tgattttatc ataatgatta ccactagttc attggatttc
    32281 gattcactaa agtccaagat aaccagttat ttacgacaaa ccgcaataaa atgaacgaat
    32341 taaaaacttt atttctcaat tagatcagca tg
//
LOCUS       DAJSKS010000013        32048 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJSKS010000013 DAJSKS010000000
VERSION     DAJSKS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.013"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            5..244
                     /locus_tag="ON396_000576"
     CDS             5..244
                     /locus_tag="ON396_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112708.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            311..520
                     /locus_tag="ON396_000577"
     CDS             311..520
                     /locus_tag="ON396_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112709.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            580..1251
                     /locus_tag="ON396_000578"
     CDS             580..1251
                     /locus_tag="ON396_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR3112710.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1304..3280
                     /locus_tag="ON396_000579"
     CDS             1304..3280
                     /locus_tag="ON396_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3112711.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3293..4048
                     /locus_tag="ON396_000580"
     CDS             3293..4048
                     /locus_tag="ON396_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3112712.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4045..4869
                     /locus_tag="ON396_000581"
     CDS             4045..4869
                     /locus_tag="ON396_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112713.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            5170..7269
                     /locus_tag="ON396_000582"
     CDS             5170..7269
                     /locus_tag="ON396_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3112714.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7307..9364)
                     /locus_tag="ON396_000583"
     CDS             complement(7307..9364)
                     /locus_tag="ON396_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HCR3112715.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9478..9690)
                     /locus_tag="ON396_000584"
     CDS             complement(9478..9690)
                     /locus_tag="ON396_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112716.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9877..10344
                     /locus_tag="ON396_000585"
     CDS             9877..10344
                     /locus_tag="ON396_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112717.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10341..10583
                     /locus_tag="ON396_000586"
     CDS             10341..10583
                     /locus_tag="ON396_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HCR3112718.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10601..10903
                     /locus_tag="ON396_000587"
     CDS             10601..10903
                     /locus_tag="ON396_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112719.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            10975..13029
                     /locus_tag="ON396_000588"
     CDS             10975..13029
                     /locus_tag="ON396_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112720.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            13029..15641
                     /locus_tag="ON396_000589"
     CDS             13029..15641
                     /locus_tag="ON396_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCR3112721.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15638..18169
                     /locus_tag="ON396_000590"
     CDS             15638..18169
                     /locus_tag="ON396_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112722.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18221..18553
                     /locus_tag="ON396_000591"
     CDS             18221..18553
                     /locus_tag="ON396_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112723.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18554..19147
                     /locus_tag="ON396_000592"
     CDS             18554..19147
                     /locus_tag="ON396_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112724.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19310..21004
                     /locus_tag="ON396_000593"
     CDS             19310..21004
                     /locus_tag="ON396_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR3112725.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21692..23569
                     /gene="ltrA"
                     /locus_tag="ON396_000594"
     CDS             21692..23569
                     /gene="ltrA"
                     /locus_tag="ON396_000594"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR3112726.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23700..24077
                     /locus_tag="ON396_000595"
                     /pseudo
     CDS             23700..24077
                     /locus_tag="ON396_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24077..25282
                     /locus_tag="ON396_000596"
     CDS             24077..25282
                     /locus_tag="ON396_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR3112727.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25298..25942
                     /locus_tag="ON396_000597"
     CDS             25298..25942
                     /locus_tag="ON396_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112728.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            25953..26759
                     /locus_tag="ON396_000598"
     CDS             25953..26759
                     /locus_tag="ON396_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112729.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            26781..27011
                     /locus_tag="ON396_000599"
     CDS             26781..27011
                     /locus_tag="ON396_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112730.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            27557..27832
                     /locus_tag="ON396_000600"
     CDS             27557..27832
                     /locus_tag="ON396_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112731.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            27874..28296
                     /locus_tag="ON396_000601"
     CDS             27874..28296
                     /locus_tag="ON396_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112732.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28315..29907
                     /locus_tag="ON396_000602"
     CDS             28315..29907
                     /locus_tag="ON396_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112733.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            29919..30254
                     /locus_tag="ON396_000603"
     CDS             29919..30254
                     /locus_tag="ON396_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112734.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30401..30595
                     /locus_tag="ON396_000604"
     CDS             30401..30595
                     /locus_tag="ON396_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112735.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            30627..>32048
                     /locus_tag="ON396_000605"
     CDS             30627..>32048
                     /locus_tag="ON396_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112736.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKK"
BASE COUNT        11821 a         4978 c         6534 g         8715 t
ORIGIN      
        1 gtagatggcg cgaccagttt tagaggcgat ggatatggat actttgacaa aaggaaggta
       61 tgtacagctg ttagctcgtg gttattcacc taaagaagta ttcaaagcct tatctaaagg
      121 aacagatatg gaaaaagaaa gattgcgaaa agaattcgat tattggagaa cccataatgg
      181 aatcaaggat ttgaaaccag cacggccagt aattaaacga aagaaaaaga taacccaaga
      241 ctgaagtgta gagcgtatgt gatcataggc ttttttattt ttgcaaaaaa caaggaaggt
      301 aggaaaactc atgaattatg aagaaatcga aaacagaaaa aaagtgtcga aagaaatgga
      361 agaaaagtta ctaaaaacga tgaagcaaaa acaccttaaa agattatctg tcgcgcaata
      421 tatcaatgat atgcagttaa caggaaaaga gaaagcttgt ttgttaggtt caatgaaaaa
      481 ctttgaacag ttgagacgaa cttatagaat tcatttttaa aactttttct atggaaatga
      541 gaaaaagttt ttttgttttg tctgaaagga ggcagatgga tgaaaaaatg gttatttggt
      601 tttttaggag tcactttaat cgtcttttgc agtgtgtttg gctatgttag ctaccaaaaa
      661 catgaaggag aggtgtttaa gcaaaatata gaaaaaaaga tgcccgtaga tcaaatcaat
      721 gcacatgcaa agagttataa ggaggatgca acgaacgtca ataatgatat gtctttgggg
      781 caaatgttgt ccatacaaaa agaagcaatc gaaatgggcg tcaataaaca agtatttgca
      841 caaattcaga ttcctgcact tggtttagca ttgccaattt ttaaaggggc aaatcaatac
      901 actttgagtc ttggagcggc tacttatttt tatgaagatg ccgaaatggg caaaggaaac
      961 tatgtcctag caggtcataa catggaaatg ccaggtgttt tattttctga tattcaaaag
     1021 ttgtcattag gggaggtgat ggacttagtt agtaacgatg gcgtctatcg ttacaaggta
     1081 acgcgtaaat ttatcgtacc tgggtacttt aaattaatcg atggcgttcc tgaagagaac
     1141 tctttcttgt ctttgccaaa gaaaggagag aaacccttat taacgttgtt tacttgtgtt
     1201 tatacgtcac aaggaaaaga aagatacgtg gtacagggag agctacaata acaaggtttt
     1261 aatcaacgcg agtcgttgaa atatgaaatg aggagagtaa aaaatggcaa taaatcggta
     1321 tgtaaaaaaa gctggaatgc ttgttggaat gttaggaatc attgcaggct gctttcaggg
     1381 gtatcaagta catgcagaag atgtcacgca aaaaacacca ccagagaagg tcaatatcac
     1441 ggttcataaa ttaatgtacg atcaaggaac gcaattgaat gttgatattg acgggatcaa
     1501 aaacgatggt tatacgcacg atcaatatcc tacaggtgta acgaaataca acaaggcaga
     1561 ttatggtgat gttgagttta cgttaggaaa tattacggat caagtattac cgagagaaga
     1621 ttctgatcta accaacgcga aagtggacga gattgtacaa gatgtagaag acaagggatc
     1681 taacagtgag tacgtcaaga atgcaaccaa caaaattact tcagctgtag atgaaaatgg
     1741 tgagatcact tttactgatc aaccagccta tgtcaattcc aaagggaatg tatatgtggt
     1801 ttatgaaagt aaatccgctg ctggattagt cacacaaaaa gcaaaaccaa tggttgtgat
     1861 tgcgccaatg acagataaaa caggatcagg atttttaaaa aatattcatt tgtatcctaa
     1921 gaacattgtt tctaagttga gttttgaatt aacgaaattt ggtgacgatg gcgcagacca
     1981 aagcaaacaa acccttttaa aaggggcaaa atttgagtta tacaagggcg aacctggaaa
     2041 aggaacaaaa ttgggtgatc ttgtatcgga tgatcaagga aagctgactg cgactgattt
     2101 gacgttagga aaatactatt ttgtggaagt tccttcggaa gttgtagtgg gatcagatca
     2161 agaaccaaca gcagatcaat atttattagg cgcagacgct cgcaacgatg cgcacaataa
     2221 attaaccttt gaaattacga atgatggcgt gacaagtgac ttaaaagctt catacgtaaa
     2281 ctataaagca ccagtgatcg ataaaacagt aacaaatgga acaggacaag aacattcttt
     2341 ccaaattggt gacgcagtaa attatcaagg aacgattcat attccaacag acattgcagg
     2401 tggcgcagac ggtatcacag taaacggtgt gaaaagcgaa acttctcctt acagtgtatt
     2461 taaatgggga gatacagcag gtaaaggact ttcttatgtt gctgaaaaag cagatattaa
     2521 agtaacaaat aatgatggtt cagtcgtatt aaaagaaaat actgactata aaatcgaaaa
     2581 ttctgatagt ggttttgtga tcgattttat cgtaaataat ggacaagtaa gtgatacagt
     2641 tgcgaattta catggtcaag atctaaaaat gacttataac atgtttgtga atgatagtgc
     2701 tgaaatcgca aatccattaa caaatagcgt ggatttcgtt tataacaata atccatttaa
     2761 tcaggaagaa caccacgaaa aaacgaaagc tgacgttgtg acttatggcg caaaattctt
     2821 aaaagtcgat agcggcctat ttggaacagg tatcaaagca acaccgcttg aaggcgcaga
     2881 atttgcagct aagaatgcag aaggtaagta ctacggtgga cttactgata cagataaaga
     2941 tggtgtcaaa gaagcagttt gggtagacga tgtagcaaat gcagcaatcc taaaatcaga
     3001 taaagagggt cattttgaaa tcactggttt aactgaagga gattactcac tggaagaaac
     3061 aaaagctcct gaaaactatc aaaaattaac aaaagagatc tctttcaaag tagacaaaga
     3121 ttcatacaaa gaagaaaacc gaatcacgat caaaaataat caaaaagctt ccgtaccaat
     3181 gactggtagc aacggtttcc aatcttacgt attgatctct tgcttgttgc taggagcagg
     3241 gggactttct gcagttgttt actttaagaa gaaagcctag aaggatcagc taatgattat
     3301 tcgtaagccc ccaaaaagaa gagtcatgca caaactgatg atattttttt ggttgctgtc
     3361 ggcagtagca gtctatttaa ttccagttgt ggaaacaaat gtagcgaaat ttcaacaaca
     3421 gcaaagaatt ttagccattg aaaatcttga aaaaacagat tcttctttgg atgttgcgaa
     3481 aatagagtta ggcgatcctg ttggtgttct tacaattcca agtatttctt taaaactacc
     3541 aatttatgac ggaacttccg acaaaatttt agagaatggt gtagggatta cggaaggaac
     3601 tggcgatatt acaggaggaa acggtaaaaa tcctttaatc gcggggcaca gtggacttta
     3661 taaagataat cttttcgatg atttgccttc agtaaaaaaa gggcaaaaat tctttattaa
     3721 ggtaaatgaa gaacaacatg cctatcaaat ttatcgaatt gaagaagtta aagcagaaga
     3781 attacaaaaa aattatttag attatcttat gcctgaaaaa gaccaagata ggatcacgtt
     3841 aatgacatgc acaccaaaag gtgtcaactc acaccgcttt ttagtgtatg gtgaacgagt
     3901 ttccttcagt aaagaagatt taaaactaca gaaaaaagca aaaaatagga aatcaatttc
     3961 attggattgg cgattagcac tgctattcgc ttttttttgc gttcctatta tttttattta
     4021 taaaaaaata aggaggagat cggaatgaat aagagaaaat ggctgtttgt ttgtgtagca
     4081 gctctctttt ctactgtagc ttattcgcaa ctgtctttcg ctgacagcag tgatagtagt
     4141 accactcaaa caatagataa ttcaattact gcaccaagcg aaacggacag ttcggagaat
     4201 actacagaag aaacggcaga ttcaaatata agctttactg aatcaacgaa tccaacgtca
     4261 gaaagttctt cagagacttc agaaaagccg caagacgtag aagatacgca agtgatcaag
     4321 caagccattc atattcaaaa aattatttct tcgaatcaaa tcaacaatca aggcagtcaa
     4381 gtagcccagc aagaaggtat caatggcgcg aagtttattg tctacgatat cacgaatatc
     4441 ttagaagaaa tgtcacaaga tgataatgag aaaaatcaat ctgtagaaat aattgagtca
     4501 aaattgaaag atcgagcaaa aaaactttca tacgaccagt tgaaaaaagt ggctgaagga
     4561 gaaaccaaaa cgattgatga tcaggaaggc gtgattgaat tttccattga agtacctgca
     4621 aacaaaaaac aagcatacta tatcgttaat gaatcaagcc ctgaaaacat ttcaaacagt
     4681 gaagatattg ttttattgac acctgtttca aatgaaaaag gtgaattttt gacggatgtt
     4741 tggttatacc ctaaatcaga gaaatcagag ccgaaagaag aaattaaaaa agttgtgtca
     4801 actggtgtaa agaagaactt ttttgaaaat tgttgggaat tttttgctca attatttttt
     4861 gataattagg atgaaatgat gaaaataata agaatattag tttttaatct atccttttta
     4921 gccgcaacaa tattgtgttg cggtttaatt ttttctcaat tatcaattta tccaagatat
     4981 caacgattgt tcattcattt tctaaacatt caatgtgctt ccatgccatt agaggtgatt
     5041 aaaaagctct tgatcaatcc aatttttatg gttagtttgg ttcttcttac aggattggta
     5101 acgtgggtat taggaaaaaa agaatgaaaa gcttacgatc gtttaaaaaa atattagatg
     5161 gagggcatta tggaaaataa aattcattgc actacgggaa gagtaattac tttagtgttg
     5221 tttttgttcc ttcttctttt tgcgagggga actgcagtcc aggccttaga aaaagttgat
     5281 ccgagtcaaa tcaaagaaaa taataatctg gtgctttatc atgatagtga ttcattgaga
     5341 aatatcgaag taactgggat caatggattt aagatactag atgagtattc aactaaaaaa
     5401 caaaaagtac ttgcttggcc aaaagtcaaa gatttgaaaa gtacgtcaaa aatcgttgcc
     5461 cattatcaaa aagtaggtac ttatctagga aaagaaattg attgtgacgt gactttttct
     5521 aattttgtag cgcacgctgg ggttaaccaa gacggattag ctaaaacaat taatggcaaa
     5581 aagtattata attatttgct ctttgataaa aatatttatc gaggattttt catgcagtca
     5641 atcaatgtca tgaatgcaaa actagaattt aagtatgcag gaacaaaaac aaaagtagct
     5701 ttgagtgaac cacagccaga aattcaatca aaaatgagat cgatgagagc agctggtatt
     5761 gatttaaacg atacatttct atccatttac agtttaaatg gacagtatca gcaacgtgtg
     5821 tcttggtata atgacaataa tgaatctgtc ggtgaacgta atattgatat gagagaattt
     5881 gttgggtatg aaaaaatggg tagcttacct tattttgtca caacagatac agcatgtgca
     5941 gaatacaaag ctcctgcgtt atcaacaaac aatttaactt caaaagtagt gggaggacgt
     6001 gcagaaaagg cttatagctc gaatgatcat ttcaccgatg ttgtaggagc tgatacttat
     6061 cacagaagtg gtgtaacgta tacgcttcaa ggcgcttccc caacattcat gattggcgca
     6121 aatacgaata gtatgatgtt tagctttgat actgcattgc tatggacacc acaaccatcg
     6181 aagcctacaa aagaagtgtt taacaaagct aatactgaag aggcagcaca caatattgac
     6241 aaaaaagtga ttccacaagg atcagatgtt tactatcata ttcaccaaaa gtttgatgca
     6301 ttaacagtca acacaatgaa caaatacaaa tcatttaaaa tcactgatac ctttgacagc
     6361 aaaaattttg atatggtatc ggatgggaaa aactatgatg gcgcattgtg gtatgttgaa
     6421 gcgaatggta cttggaaaaa agctgataca ggaggaaagt taactgtatc aggtaataca
     6481 gtcacttata ctgcttctga cagcttttta gctaacgctg caaattacgg tagggattat
     6541 attttgcagc tccatatgag agcaaaaaat gatgcaaaag gaattgcacc aaatgtggct
     6601 actgtaacgt ttaacaatga tccgaacaaa ggaacacaaa aaacaaatac agtagaaaac
     6661 tatttctctc ttaaaccaaa taaaaaagtg gaacaagatg ggaaagatgt tgatggtcga
     6721 aatgatggta aaaaaggaac gcctacagct cctttaaatg caggatctga agtacaatat
     6781 ctagtcacac aaaaatggca tacaaaaggt gtagacattg cctcagatca ttacaagcaa
     6841 tttagtattc aagatccagt ggaagatcgt ttgacgtata aagaaggttc tgcgcgagtg
     6901 atagatatga gtacaggaaa agatattact tcagaaggta cactaaccta tgatagcaat
     6961 tcaagaacgc tgaagtggga agcttccgct gatttcttaa gtaaaaatct tttagatgga
     7021 cgagaaattc agctgatatt tacagctaaa actcctttga atgaagaatt ggatatcgac
     7081 aaccaagcaa aagtttcagt agaaagtatt gagaaagcaa ctaatgtagt aacgattggg
     7141 gtaaaaccta acttacctca gatcattgtg ccaaaaactg gatcgaatca tttagtgcta
     7201 actgttattg gaagtatatt atttcttact ttagcatcat tgggctatgt agttttaaaa
     7261 ctcaaataaa tataaaaaca aggctgagaa tcagccttgt tttcttttat ttgaagattg
     7321 gattcgcact aataaataca ttatctacgc caattctacc agcagagtca actacaaaat
     7381 tatgacctaa tccgagaata ctttggttat tataaagtaa atttgcatta tgggcagggc
     7441 tatttttcca tcctaccatg gcatcgatag caccatcgtc tgataaaagg tcatcaatat
     7501 acagcccagt ttgacttatg ttttctccct tagcaccgta gcagcctaag ctttccaata
     7561 aatcttggaa agtaccacca ccagggcgat agtgactgaa gctgacttgt aattctttag
     7621 ctcgaatatc tgcagcctgt tgaacaatat cattgaattg cattggggca acaccatttt
     7681 gttggcgata atcatttaac caagcaagtt ctttttgacg cataaagtta cgtaattcgt
     7741 ctaatgaaat ctttttatat ttgaatgtga ttgttttatt ttgatcgacg ttattaatgg
     7801 atcgttggcc gtttttatcc acggtaacgg taggatcgtc atttctgcac aagaaataat
     7861 tttgatcagt catgtttttc caaggagtag caaagctatc cccttcgtat actgtttttg
     7921 attcactaga gagaacagcg ttggtatcag catttacatg attaacagtc acattaaatt
     7981 tacgagtgta gttgaaaata taattttgac tttcagtcac attgtttaag gttactgaat
     8041 ttgttggttc tacccgatcg tctaaaacat atccttgaat agaaggagca gttaaagtga
     8101 cactgtcacc gtgagtgaca ttgactatct gttctttcaa aatattaact cgtccttcgt
     8161 ttttgtagtt gttattgcac aacaaattga tttgatgcac ttgattataa ttgaatgtga
     8221 ttgttgtatt acctgtgatt gttttgcttt gagtatcatt accaatcact ttgtagctaa
     8281 aattatcttt agcgccttcg ctatatttaa atgcttttgc cttagcagtg taagtagaac
     8341 cagaagttac ttttgcttga tctgtagcaa tcacttgacc tgtttgattg tcaatatggt
     8401 taatagtaac cgtatattct tttggtgttt caggtttgtg ttgcaaccct ttaaatgcag
     8461 catcaagact atttttagca ttgtttactt gtgtttgaga aaccttagaa tcgtctagta
     8521 cttttttagc attggataaa gcagtctgaa aattattcca agattcttct gtaaaatctc
     8581 ctttagcagt tgattttact cggttgtata aagcttgtaa ttcagcttta ttcacagtag
     8641 gaactggttt tttctttaat cctttgtaag cagaatctaa tttatttttg gcactatcca
     8701 cttgcttttg agtcgctttt gagtcattta acactttctt agcattgttt aaagcggttt
     8761 ggaatgcgtt ccaggtattg tcggtatagt cacctttttt cgttccctta acgctcgtat
     8821 aaagagcttc tagagccgat ttattcactt tgggtttaga ggtgtcagcg ccataccaac
     8881 cgataccttc gtatttccag ccatagtctt tggttaacca ttgtacttct ttccaacttg
     8941 cagtgtagtg atgtgagcca gattttacac caggaaggaa aaggcggtag atcggtttat
     9001 tatttttttc ggcagaacgc caggcaggcc cttcataagt ccagccgtaa tttttagtca
     9061 accatttcac ttcatcccaa ctagctgtgt aatggtgatc acgaagacct ttattgtaca
     9121 aacgatagac aagatggcca ccgctagtcg gtgcttccca ggcggggcct tcataatttc
     9181 cccattttat ctttactaga tggtcacgtt cttttttgct agaggtgtaa aaatgttcat
     9241 tattatttgg attgtaaagg cgatacatga cgttagttga agcagcgtca acagatccag
     9301 caagactaag tgaggcagca aatgttccaa tagctaaaac agtttttgga ttaaataatt
     9361 tcattttttc aactcctaaa tttaattaaa aatgtttaca tgattattat atatcggcta
     9421 accgatatag tcaataaaaa ataagaaaag ccttatataa ttttggcttt tcttatttta
     9481 tttgttagtt gaagtattaa tatcaacgat ttcttcagcc aatttattta atttcatagc
     9541 atttatgaat ctcatgttgt ctatactaga gcgtttgttg gcaaggtcac ttactgtagt
     9601 taagggtacg ccagattcta tggatataga acgaactgat ttaccactat caaacagcaa
     9661 ccattcgatt ttctttttat cagcgtacat ttcaatgctc cttcttgttt tttctttatt
     9721 atagctcttt tttgtaatga atcacaatat caagagaaaa attttctata gaaaggtgga
     9781 tttttatgag tattcgttct ccgtgtatcc aaatgaggtt tgttgctact ttcgttattt
     9841 gatatactaa aaataaacaa cgaaagaagg aataaaatga agaaaaagca actatggttt
     9901 gtctctatgt ttgcaatatt ttttattatt ttaagtggat gttcaagtaa taagaaatcg
     9961 gaagctaaag aagataatag tttattgcaa ccaggaactg tttggaaaga agaagtaggt
    10021 ggtcttattc ataatttaaa aattatagat gatactacct gggaatattc tgaaagtgta
    10081 tggcatccag atcctgtaca aataacagtt aagaggcaaa aagactatga aggtttagaa
    10141 agatacaaaa tagtagattc tgcaggcatt agggaattta taaacaaacc tgattcatta
    10201 tttattgttg ttccctatga gaaggatggc gtaaaaaaaa taatttttct tggatctagc
    10261 aaggatgaga agcgaacgaa agagaaatta attcatgatg gatcagagtt aaataaatac
    10321 aaattacaaa agactagtga gtgattgcta gtctttttat ttatcctgaa ggaggaatca
    10381 cagtggcaaa agttaaaaca ccattcgaaa aagccgcgtc taaaatctta gaacgccgtg
    10441 gtgtttctga aatacagtgg aaaaaagaag ttcttgaaaa agcacaattt gacctattac
    10501 gaggaaaaga taaggactta gaagaatata tctatcaacg tgaacgtgaa aaacttgtat
    10561 tcgatgaaat tattaaacat taaaaggagc gaataatctt atgtttgaat caattacaat
    10621 gaatttttta actcaaccga aagtagtagc tgcagttgat atttttagag cattacaaac
    10681 tgccgcaact agtttgaaaa ataatctgat gatttttgcg gctgtagctg ccgttgcctc
    10741 cattgctctt ggaggtattt tcttcttctt tgggcgaaga ggagctgaaa acgggaagac
    10801 acacgtaggt tatgtattgt tgggggtagg agttatttta gctgccgctt ctattgtagt
    10861 ttcaatgttc tcaatgtttg gacaaacccc acaaggacta taaaaaaaac aagtaggagg
    10921 agtaaaaagt acgtggctac tttttactcc tttttttatg aaaggagaat attcatgttt
    10981 tttacgattt caggaaatgc aaaattggat aaagagttaa taagaaaagc aacgaagaaa
    11041 gtgggaaagc gtacttcctt tttaccgaaa acaattaaat attctgtcat tgaaacggaa
    11101 gaacagctac atgtgttagg acaagtgtta gaacaagaga atcaatcttc tgttaaattg
    11161 actctcacgt tacatgataa agaaggaaaa atagaaaagg ctggcacaga atacgttttt
    11221 ggcccactgg attatcaaat ttcagaaggt ttttatccta cattgataga tttaatccgc
    11281 cttgatgtgt ttaaagatac ggatgaaact atgagtgtgg aagagtctta tcgaaacgaa
    11341 aaactgctac aagttttaga agaagagtta gagagcgacc aaagaaaagt gaatcaagtg
    11401 agtgaagtcg aagtaaatga agaagtacca tgggaacgac caatctttcc aaagaaggag
    11461 tcaaaagaaa aagagacagt ctatgttgac gaacaagctg atattccgac tcagaaatcg
    11521 atatttgagg aagagaacga tgtgatgggt caattggagg tagatccctt tgaagaaaca
    11581 atggaggatg aagagacgga cgaagtagaa gttgatccag ttgaagctaa tgaagagtca
    11641 acatggactg aaggggattt tcaggaatat attgaaccac ttatcagtga agaagtagaa
    11701 aaaaacgaaa cagagataca aaaggcaact gtatttgatt atattttgga tcaatatccg
    11761 ccagagcatg aatggatgaa agaaaaaaac aaattatttt taaatgagct ttattcgaaa
    11821 tatcaattac cccaaaccta tcaagcattt ttagagagta aagaagaatt aatctcacag
    11881 ggtaaaggca cacttttaga acagttagag tctgtccaat caacaaattg gcatgagctt
    11941 gcacaagaag atttattagc cacctttgaa gaacgtcaac aagaaacaga tcaagaaatt
    12001 aatgagtatg cgacggaaca gtcgtctaat tgggagaaag taaaagagga actaaataaa
    12061 gaagaacagc gagtgattga agaagaagta caaaaagtca aagagcggta cgaaaagaaa
    12121 cgcgacaatg cttattcctt gtgtgtagaa aagatcaaca attttactaa gacgaatcaa
    12181 gaagcgttat cgaaagaaaa agaaaacttg ttaaaagaac gtattaaaga acgaaaagaa
    12241 gagttctacc gcggtttacg tacagataag ctaaagatta gcaaagaatt aaatgaacaa
    12301 ctgaataagc tatatcaaac aacgactgaa gttcttatgg aaaaacacga acagatccag
    12361 caagaattac aagtacaaat tccaaaatgg aaaaaagagt atgaaattca acaacaagaa
    12421 ttgaaagaac aagaagaaaa acaacggcaa aaagaaaaag agcgtgaaca attagaaata
    12481 agaaaacggg aacaggatct tgcggaacaa aaaatggcga tagaaaaaca acggttagag
    12541 caagaaaagg aaaaagaagc agctgctcgt aaggaacgtc aagaacaaat ggatacattg
    12601 cgtcaagcac agttgaatta tctagcacaa cctcaaccag tagctgctgt tcaaacgcca
    12661 gtgaacaacc aagcaaattt aactccttca ccccagcgtt cctttaacgg atggatggtg
    12721 ggatgtattg caagtttaag cttgcttcta ggtggaggaa cagtatttgc ttttaatcat
    12781 atcaatcaat cttcacaggc tgattctgta gccacaacgg cgtcaatcaa ggaagaagcc
    12841 caggaacaag caagaaaaga gtatgaacaa caattagaag aattattgaa tcgtaccaat
    12901 cagtcaacaa cagagtcaag catggaaaat agcaaagaga gtgatcctag taaggctaaa
    12961 gaaggatcaa gcgattcaac tgtacactca aacacttccg aaagttcaga atcaaatgag
    13021 gtgaaataat gcgtattaga gaaacaacga gacgaaaaga caaacgattc aatttagaaa
    13081 agaataggta tctttctcat ttctatcgct atactccttc aggagaagag ctaccagaaa
    13141 aacatgacaa acgtatttct caaaagattt tttatagtgg cttagcttat cttcctttaa
    13201 actatgttgt tacaagaata ccagaagcat ttcatcgatt tgagattatt ccaggaagtt
    13261 tacaaggagt gaatattttt cagttacaag aaatgccgca gttattccat tttgattcaa
    13321 ctggtttttg gaatcctttg accccaacaa caagtttagg cacacgttta gttgtttcta
    13381 cgatttgtgt tgttccagct gtacttatgg gattgaaaat ggacatgtgg tttcgtccgc
    13441 tagcagatgg acaaaaaggg gataatcgat taatgacctt aaaagaaatc caacagattt
    13501 atccgcaaat acctgaaaca aaaactactt ttccaggtta tggaggtatt ccaatcacac
    13561 attacaaaaa atattggtac attcaaacag atactgttaa tacgtgtatt gttggtacct
    13621 ctcgatcagg gaaaggtcag attgaagtgt tagccaccat tgacaattta tctcgtgcag
    13681 aaaaacaaag ttcaatggtc gttaatgatc caaaaacaga attatatgtt ggtagtaaag
    13741 atgaactaga agctagaggg tatgatgtat atgcctttaa tatcaccgat ccactgcaat
    13801 caatgagtga taatccattg gcactcatcg tgaaatactg gaaacgagga gacatagata
    13861 ctgcaacaca gttaaccaat actttcacaa atacgattta ttatgatgcc aacgcttcag
    13921 ataacaaata ctttaacgaa aacgcgcaga aagctgtcaa tgcgcttatt tttgcgttgt
    13981 tagaacaagc tgatcaaaca ggagattact ccaaattgac accgaataat ctcgttgagc
    14041 ttctttctga gattggcggt tttaattatt atccagatcc agacaatgaa ttcaaacagt
    14101 tgaatgcctt agatgaattt atgaatcagt tgccacctgg aaacattgca aaaaaacaat
    14161 atggggcaac gaaaattgga tctgataaag cgaagggaaa tattttatca acggcaatca
    14221 cgggattgaa tccatttact ctaacgaaaa ttgcaaagat gacttctcaa tcaacgattg
    14281 attataaatc ggttgggttt ccaaaatact tagatttgaa attacatgaa agtcttttaa
    14341 atcaacgagt cgtagtagaa ttttatcata agggaaagaa atttcatgaa gaacgtgtca
    14401 aagtaggcta taaaggattt agtgaaatca attttgattg tcatttagtt gcaggtgatc
    14461 aagtttgttt aaaatacgat catcaaggaa aagaatatgt ggcttggtat gaattttctc
    14521 aaagagtatt gaaagacgaa gagggtaata tcgtttatcg gaaaaaacga ggagaaaccc
    14581 atttgccaga attagaaaaa gaagccgttc taacattaga tgaggaaaaa agtaacttat
    14641 tagtagagtc tattcggatg cactataccg ataaaccaat ggctattttt atgcttacac
    14701 ctgattatga tccatctaac catatcattg ttagtatttt tcttagtcag ttatataaag
    14761 aattatctac gcaatgtgtt aagactaaag gagataaatg tcaccgcagg attcatttta
    14821 ttttagatga atttgggaat atgccaccaa tggataatat ggaaggaatc atgacggtca
    14881 cagctggccg aaacatgttg tgggatctgt ttattcaatc ttatcagcaa ctttatgcga
    14941 agtatggaga acaaaacgca gcgattatca aagaaaattg tcagacacat atttatatta
    15001 tgtcgacaga tgataatacc atcgaagaat tttctaaaaa agtggggaat aagacggttg
    15061 aacaagagaa cagtaccatc aattcattag gtatgaatac tcatatcaat cgcaatgttg
    15121 attcagatcg tatcttaact cctgaaagaa tttctacttt tttagaagga gaaacgattg
    15181 tactcgctcc tttaaaacga caagatttga aaggtcataa aatacggccg tatcctattt
    15241 ttaatcatgg ggaaacgaat cttccttatg cttatcaata tattgaagat ttcaatccat
    15301 cgtgggatat caatgatata gacatacatt gtacacatgc ggacttggat cttaaggctt
    15361 tacaaattga ttatgattat ttcctaccag atgaatatgc tagaaatctc tatcatggac
    15421 tgcatgatca attagaagaa aaaaacgaag aaaatatgtt ttataaaatc gttcaatctt
    15481 ttgagaatga gacggctaaa aaattgttaa ttcaatttta cgaagaacaa gcagcagatc
    15541 gacttcaaga atatgttgaa aagttgatca aaaataaaga aatttctgaa atgattggga
    15601 aacggttgtt attggaatct gaaaaggtgg tgaaagaatg atcattggag gaattttttc
    15661 tggcatagcc gactggtggt caggcttatt cggagaaggg gatgcaaacc ccaaaaccgt
    15721 aacgaaagtc ttacaagatt atgggaatta tctgcagtat caagatgtat ttagttggat
    15781 ttggaacact tttctgtggg gattgattaa aggattatat aatctcaata ctttactaga
    15841 aaaaaccatt tatcaaagct ttgatttaaa agacttattg aacgccgctg gggtaaatgc
    15901 actatttcaa agtttgatta gtaaggtatt ggctcttttt tgtgtcgtca cattaattta
    15961 tttaggcttg aaatttttta tgagtaaaca cccaccaaaa gtcaaaaata tcttggttaa
    16021 tttatttatg gccatgatct tgatttttgg tggtagttct ttaatcgatg aagggttaaa
    16081 tatcagtaaa agcttttacg gagacgtcac tgcagcaaat aaagcagata aaacgggatc
    16141 acctgctttt caaattattc aaaataatgt gatcgatgtc agtacgttac tagcaactga
    16201 cccgaataaa gtagaatccc taccaacaga taaaagaaat gctttaacag cgaaaaattt
    16261 taaaacagcg aatatcaatg ggattattac tcctgaacaa gctaaaggta tggcaggtaa
    16321 tgataaaagt gggttatcag atatacaaaa ggatcggtta aaggcattac aataccgatt
    16381 ggaactagat gataatggga aagaaattcc tgttcaaatt agtgacgaag gcttagcaaa
    16441 atatgtctat acttcagggt atcgtcgata cagtagtaaa ccaggtatta ttttagctgg
    16501 tgaatcttct ttggcagtag cctatttatt tatcctattt acgatcgtta cttgcattat
    16561 tgagctggta tttaaaaaat tctatttagt gattgccgcg tcaacggact ttgagacagg
    16621 tcaacggatg aaaagtgcga tagaagatat ttctcaatcc ttcttgttat tggcgtttac
    16681 gattttagaa ttacgaatct ataccttaat gctttcaggt atcggtgatt tgcatgcggc
    16741 aggaaagatc aatggattct tgtatgtcgt tgctttgatt gtactaacca ttgcgctatt
    16801 caaaggctct caatcagtta cgaaaatttt cggcgttgat acaagcctga aaaacggcag
    16861 taattctctt cttagcatgt ttgccttagg aaatattgcg aaaaatgtgg gtagcggagc
    16921 taaaaacctt gcaagtgctg gtaagagtgg tttaagccat ttaaatgata ttcgtaaaaa
    16981 tggatttggc catcgaaaag atgttacccc tgataacggg gaagaacaat cagacgataa
    17041 agctacaact gtacctaata gtaagaaaac agtttccgaa atgttcggga aagcgaaaaa
    17101 tggtgtttct aaagccgcag aaggtcttgg ttacgtcaat gaacgtgggt taaaaggaac
    17161 agctgaagat cttacagaaa aagccaaaga caccttagaa gataaattag acggcacgag
    17221 agctaaagaa ttggctgaag ctgtgaagaa tccatccgaa gcactagaaa aacaaaaaga
    17281 aaaagcttca gaagtcaaag aaggactaaa agatgcgtat ggtgaaggac aagtcaatgc
    17341 agcagtgaaa gctaatacaa caaccgaaaa agaaaatcaa gaaggtatag tgattccttc
    17401 agtcaaagat caccaagaag cgttgcactc taatgaaaat tcgattgaag agaaaaattc
    17461 catagaagaa agaaattcag aagaaggaaa aaatggacag ttaccaaaaa ctgatccaga
    17521 acaatatcgc tataaaccta acagtgacct tgaaaatgag aagcgaattt cagatttatc
    17581 aaaagtaaat caagaagaca aagcagaaaa tgagggacag ttaccaaaaa ctgatccaga
    17641 acaatatcgg tatagatctg ttaactccga aaatgaaaaa cgcatttcag atttaccaaa
    17701 acgaaatcaa gaagtacaaa cggatgagga ttccatcgtt ctgccaaatg ataatggaat
    17761 cgaagttgga actgaaaaat caacgaaaat tggtttacct aaggcacaaa atttaccaat
    17821 gaaagatagt cgtttgaatc aattcaagga taatgatcaa agttttgttc atcaatcacc
    17881 tgcaggtact gttcaaatga aaccaagtcg tttgcctgaa aatggaaatc tgacaactgt
    17941 ttcttacaat ttatctgaaa gtggtgagac agggaatgcg tccgttcaaa atgttgtgac
    18001 aagagaagct gcaccaaaac ctattcaaaa cgttcataca ggttacaagc cagcaaatgt
    18061 ggcatttact gcaccaacaa aaataaatgt gacaagagaa acaaaaaaca ggaacaatgc
    18121 attgaaagaa caactattga gggatatcaa acaggatatc aaaccataaa aggcctacaa
    18181 gttaggcctt ttttctatgt atgaaaagga gaaatcattt atggataaat atagtaacat
    18241 gaaaaaccct ccaagattta gtggagaatg gaagtttttc gcttttttct ttttagatct
    18301 attaacgctt ggtggctgct ttttttgggc gaaaatggtc aataatcttg tccacttacc
    18361 actctttttt gcactcttga attatggctt atggattgcg atagcgttag tgattgtttg
    18421 gcgaccctat aataacccag gtcaacgaca aatttttgcg attatcgagt ctattttttt
    18481 tgcggacaag aaccattatc atgcgttaga tcctaaccga aattatcatg gtacgtataa
    18541 ggaggatgaa taaatgcgag tcaaaggaca aaattcagtt gaaaggaaat ccattttgca
    18601 gtttggaaaa ccagaagagc gctctatctt ggacttattt ccatataaat cctgtacaga
    18661 agagggagtc ttaattacta gagaagatca ttttcaacgt tattatcgtg tagtttctac
    18721 agacgtggaa ggattaaatg aacaagagaa actagaacga atgaatcagt tgaccaccgt
    18781 catgcgtacg tatgtgccaa caattaagtt agttagtttg accacagaga cggatctaac
    18841 ggaacaaatc gtgcaaaagc ggcaattatt agataaaaat cgtatggaac agtccttagg
    18901 aagaaatcta caacgattaa aaaaatatga aaagaaatta gtcgaagaaa tccaggaatt
    18961 gaaacgagca gaacaagaac gtcctgactt aagcttcttt ttcgtcattg aaggaaagac
    19021 aatcaaggat ttacaaacca gaaaccgtca gttaatgcga tctagtggca ttttagggtt
    19081 aaaaccactc tacaaaaaag aattgatcaa gattctgtat cgaatgaaca atatgaatga
    19141 tgagtaggaa tggagaagag agaatgaaat atccgttgat atggttagaa aaagatgttc
    19201 cgaaggaaga gaccactaga aaatttcctc acactttaag cctgggtaag ttgggaaaag
    19261 ggcaatcttt taaccaagta aagaatcaaa caagaagagg tgactgtaca tgttagaagc
    19321 aattaaaaca gctttttcac cacaaaatga tcaaaaagta cttgatccat taaagcaact
    19381 aggaaatcgg gaaatagaaa ttgtaaaatc caaagggtat gatttagatt ttttaaaaca
    19441 agtgatgcca caaggtgggt taaattttga tgaagaatat gtgacgtatg gtaatggtta
    19501 ttcacgatgt gtgacagttt atcactacac aaaaaatcct acgctttttt ggttggccaa
    19561 tctcatgaac aatcctcata cagtggccac actcgatgtt agcacagatg aaaagcaaaa
    19621 agtgattgat caaatcaatc gtgccttaga tgaattaaat gatcgatcag ataatgaacg
    19681 aaaagcgact gaccgaaata aaagcgcgga agaatacctg gaactagcgg aatatgctag
    19741 aaaattgact tctaatgggg aaatttctaa gcaagtcaaa gtgagagtac atgtttacgg
    19801 aaacacgatt caagaagtag atgaacgagc gcaggaactg atcgaaaatc tcaaagggat
    19861 ggattatcgt gcggtgactt atctgcataa aaccaaacga gattggcaat cgatgtttac
    19921 catgttcgat gaacaagctg aatggttcgg aaatatcaaa gggcaatcac taccatcgtt
    19981 aaacattggc ggaggtttcc cgttccacca ccaaacgcta aaagatccag gagggacaca
    20041 atatggggaa accatgacag gcggcccatt tgtctggaat gcgacgtata aaaatagtgt
    20101 acggttgtct actaatacct tgcttctagg aatgatggga gctggtaaat cgaccgttct
    20161 taaaatgatc gcggaagccc atttagctgc aggagacttt atttggggct ttgaaaaggg
    20221 aaaagacttt attcctttct taaaggaata taacggtatt atggtgcgtt tagacggatc
    20281 tgacggaatg atcaatcctt tagagatatt tgccacacgg acgtatgatg aagcatccag
    20341 tttatatgac gatggatcag tcaaagattt gaagatcaat gaagcagcgt cctatcaaac
    20401 acacttagac aaagtggtct atcaggtaca gttagtgagt cctcagctaa aaggaacgat
    20461 gaaagcagaa tttaagacct acttaaatcg tttttatgaa gagtatggaa cagtaccacg
    20521 cggctttact tcctcaaatt cgcgttcaaa tactgatacg caagtcacag ggaaagatcc
    20581 agaagcttat ccgacgttca aagaattttt agacttttta ggacaactgg aattaccagg
    20641 tgcttcccaa gagaaaaaga atcgaaaaga agaaatggaa tccatcgtag aatccttatg
    20701 tgagacgtat ggcatgattt ttgatggtca tagtacgatt cgacaccttg accaacaaca
    20761 gttggtcttt tttgacattg actctattac aggcttagat cgaggggtac aacaatgtca
    20821 aatgtacatg gctatgacgt tgatttggaa tcaggcattg attcaaggaa gaagacaacg
    20881 acgtttaatt aaagaaaaac aaatggaaga acaagacttc aaacgcttct tggcgatgat
    20941 tgatgagtgc cataatggtg cgacctgttc tgcagtgaaa agctgtgggt tgcaagtagt
    21001 ctaaaaacta ttgtaataac tcttataacg agaagtcaaa tgagtgggta acgccacaaa
    21061 gggcttccac tgatactccg acatgcaaca agtagtttat gcagaccaac gttgtgtgaa
    21121 gctcggtgaa gtcggctgaa ggataccgta aggtaaaaga aatagtgaat accacgaaag
    21181 ttctctagag gtaaagggtg tatccgataa gtcgggtgtc caaaaaaact taatatggtg
    21241 agtatgtaca aacgtactga cgaactagcg aatgtactgt ccgaaaggca tagctcctag
    21301 aaatgggaga gacagtgagc aaaaaatcac tcattgtgct aacttgtggt tgagtaaaag
    21361 tcgttgttat gaaagaccat gctgcgttac aggcgtagga gaagttaggg ggcaaaagct
    21421 agcatctaag ttaaggcatg tggatagata taagggaaca ggggaagcca tcaacatgga
    21481 gagtgcatac tcgatgaagt ggtagtaaga aatatgtgaa tgacaaataa cttactttag
    21541 tgatggtgaa agtagagtca cgactgatga aggttctgta atggaactgg aggaacaggc
    21601 tcgattcgtc tgtaaaagtc atgaaacccg ttcaaccatg ggattctcgt atgactaaaa
    21661 aggtcacgag ccaattggag cgtgataaac catgacaaaa gaaacaagtc caaaagatag
    21721 gaagttgcgg catgcagaat attatggcat gacggaaatc ttcgataaga tatactccaa
    21781 aagtttgaag ggagataatt tcaagaatct aatgcagatt atcatttctg ataacaacat
    21841 tgtcctagca tatcgtaata tcaagagaaa tcacggtagt gtgacaagag gagaagatgg
    21901 agtaacgatt aaagatattg aaaatctcaa ccgtaatgaa ttcgtagaaa ttgttaggaa
    21961 gcgattcgca cattataacc ctcggaaagt aaggcgtgta gaaattccta aatataatgg
    22021 taaattaaga ccgttgggta ttccatcaat atgggataga atcgcgcagc aatgcatctt
    22081 acaagtatta gaaccaatat gtgaagcaaa attcaataat cacagctatg gtttccgccc
    22141 aaatagatca gcagaacacg caatagcaga ttgtatggcc cgaatgaatc aaagtcacat
    22201 ggaatatgtg gtagatgtag atatcaaagg tttcttcgac gaagtcaatc atactaaact
    22261 catgagacaa atctggtcta tgggtatacg agacaaacag cttctcttaa tcattaggaa
    22321 aatgttaaga gcacccgtag tacttccaaa tgggaagttg atatacccaa caaaaggaac
    22381 accacaaggc ggaatactta gcccattact tgcgaacatc aatctcaacg aatttgactg
    22441 gtggatagca aatcagtggg aggaaaaaga tgccaaagag ctaaaacatc attttaggaa
    22501 aaatggagct tgggataaag gtaatatcta tcacaagctc agaaagagta caactctaaa
    22561 agaaatatac ctagtgagat acgcagatga ttttaaaatt ttctgtagaa attataatga
    22621 agcgaaaaaa gttttctatg caactaaaat gtggttacag gagcgtctaa agttaccaat
    22681 atctgaagaa aagtcaaaaa tcacaaatct ttcaaaagaa gaaagtgaat ttttaggctt
    22741 tacgatgaaa ttagtgacca aaggtaacaa aaaggttgtt aatacgcata taggtacaaa
    22801 agcacaaagc atgattaaag tgaaactaaa aaaacaaatc aaacgaattc aaaaaagtcc
    22861 taatagcaaa gataccttta tctatattgg agtctataac agtatggtta ttgggattca
    22921 taattacttc aaaattgcga ctcattgtag tgtagcaatg cgaaaaattg caagagaact
    22981 cagaccaagt tttgaaatac gactacgaga acaaggaatc tcaaaagaag gacaatacgg
    23041 aaagtacgaa ggatacaagc cttacctacg gtcaggacaa atacgatata taagagggtt
    23101 tccaatatta ccagtagggt atattcaaca cagacatccg atgtgtaaaa aaagaaatat
    23161 taataaatac accgaagaag gtagggaagc cattcataag aagcaacaaa ccgtagaaga
    23221 atggaagata tcgtggttgt gtagcaatcc agtgcttagt aagagagcaa cgattgaatt
    23281 tgcggacaat cgaatttcaa aattcatcgc tcaaaaggga agatgtgcag taacaggtga
    23341 ggagcttatc ttatctgaaa tgcattgtca tcatattatc ccttatcatg aaagcaaaag
    23401 tgatagctat gaaaacctag tgattgttac tgaggaggtt catcgggtaa tacacgctac
    23461 acaaagtgaa acgattgagg aacttctaaa atatctaaaa ttaaatccaa aacaaaaaga
    23521 aaaactcaat gagttgagat taaaagttgg taacgaagaa atatcttaat aaatatttgt
    23581 aggacgggaa aacagaatgt cttttataga cgagggaacg ccgtatgcgg tgaaagtcgc
    23641 acgtacggtg tggactgggg gaaaagacgg agataatatc aaactcttac ctatcagtat
    23701 cgattaatcc agattttatt gaaacggtaa attatgtcac aaactttcaa cgagaaatgc
    23761 gtaaagtaga agcaatgact gttttagcta cacaatcccc acaagaaatg gtgccagaaa
    23821 atatgtcttc tgataacttc tcgaaactga agaaagtctt tgaattttct tctacgaaga
    23881 tctttatgcg tatggatgaa tctattttac gtcatataga aggattagtg ggtaaatcaa
    23941 tgacgaactc agaattagaa tccattcctc aacaaaatca aggagacgct gtgatcaatt
    24001 ttggctcaaa agaaaccatt cgtgttcatt ttagtccgaa caaacgtcaa ttaaaattgt
    24061 ttgatggagg aaaataatgg aaaaccaaaa tgaaagttta atcaagcagt atgttaagcg
    24121 tcgtgcaaag cggcgcttat ttttgtggtt gtttggtact tctgcaggtt tgattactat
    24181 tttgatcact gtgttcgtga ccctatttct aatacttgcg gcgggctcga ttgataattc
    24241 ggattcagat tcaagcagtg gtggcgaagc ttttacagga gaatattcgg aaggcttacc
    24301 gatttataaa gaaatcaaag gaagaggccc tttttctgat gagattgcgc aatatgccgt
    24361 aggtgcagca gtgaaatata aattattacc aagtgtgatc ctttcgcaat acggttacga
    24421 atcagcgttt ggtacatcgg cgtccgcaag aaatgatctc aactattttg ggattacttg
    24481 gtttgatggt tgtctgtttc ctaaaggaac tgcacgagga attggtggca ttgaaggtgg
    24541 ctggtacatg aagtttccca actcaaaagc tgcattttcc tattacggct ttatggtagc
    24601 cacacaatcc aattttaatg cttgtgtagg aaataaaagc cctggcgcaa gcttattgat
    24661 cttaggacga ggcggttatg ccgctgcagg aattacggaa gatagtccgt attatacagg
    24721 ttgtatgtca attatcactt ctaataaatt aaccgaatat gatgaattcg ccatcaaaca
    24781 ttggggtgaa ggtggcaata acaacggcac gattactggt gaatggacga atccattccc
    24841 tggcagcagt ttagataaaa gttccttcag cggtggtcaa ttatttggaa cgaatccagg
    24901 cggtgaattc cgtccgaacg gcttccatga tggattagat tttggttcag ttgatcatcc
    24961 aggaagtgag attcatgcgg ttcatggcgg aaaagtcgtg tacgttggca atcctggtat
    25021 ttcaggtttg ggtgcgtgtg tgatcgtgat taattacgat ggcttgaata tggtgtatca
    25081 agaatttgca aatagtacag gaaattctcg agtgaaagta ggcgatcaag tgaaagttgg
    25141 tcaagtaatt gggatacgag atacagcaca cttgcattta ggatttactc gaatggattg
    25201 gcgtcaagca caaggacatg cattcattga tgatggtaca tggatcgatc cgttaccatt
    25261 tttgaatagc agtaaaaaat agatcaggag aaaaaatatg gaaaataaaa cgttaaaaat
    25321 cgtcttaaca gctgcaatta gcgttagttt aatcggcaat attgcgttgt tttggcagct
    25381 gaaagaggaa caagcagcaa atacctataa aattgaagaa gtggctaaaa acgcgtctga
    25441 gaagctcaaa aaaaattcct ctgaggcaca ggcgaagatc gacaagttaa atcaaaccat
    25501 caatgagaaa aaccaagaaa tcgatcagct gaagcaacaa gatggcacaa aagcaaaaga
    25561 ggaagtaact gaagcacaaa gaaagacggc agaagagttc ggtcaattgg ccattgataa
    25621 atcattaagc agaaatgaat taacggaaaa actaaaagat gtcgcaaccc aagaagtgat
    25681 cgacaaatta agtcctacag acgatgatca ccaacatgat gattacggaa gttactcctt
    25741 tacattaggt gacgtgcaaa cttacgctca aacaagcagt gtaaaagagg aacaaaattt
    25801 tgtcgtattt gttgattata caatcggcaa tccgcaattc aaagatgtca aaccacaaaa
    25861 aataaaaggt ggattaactg tcactgaaaa acaagtagat ggtcaatgga aagtcataga
    25921 ctttagttat ttcactcgtt aggaggaaga aaatgagtaa gaaaaaagga gaaagacaac
    25981 gtttcaaaaa acgaaaaagt tatcttagat ggctgggaat ctttatgatt ctcggctttt
    26041 tgattcttgc tagtccgatt tatacgaatc agaaggtaga tataccagat gaggtaagca
    26101 cgaacgcctt tttaaacacg aaagagctga caatggtttc aaaagaaaaa ggagcgaacc
    26161 agcaattagt ttttgaattt gcgatcgatg atgaagatca aagcttgtta aaagaactgg
    26221 ctaatcttaa ttatcgcgta gaagtaaaga cagctaaagg ggattatcaa gcaatacgag
    26281 ccaaagtgat taaggtgtct gatgattatt ttgcagtgaa gctaacgcat gtaccagaag
    26341 aatatatcgc catgcgttta accattattc ctgaaaaaat tgatccaaaa gtggatatgc
    26401 aagaaccaca agatctgatt ttttatattc acgaagataa ggtcaaagat cacgtgaaag
    26461 acgaagatta tgaacaacat gcgatcaatt acaaagtgaa aggatacaag aaagaacaaa
    26521 aagcagctca aaaacaaatg gaatctttcc aagcaaccat tgatttgaat gaagaattag
    26581 tgaagaagct aaaagatcaa ttgccttatc aagtcgcaga tgatcaggaa aatactgaaa
    26641 acaagattaa tagctaccag caagaaattg aaacatcgaa aacgcaactg aaagatcaag
    26701 aagagatcat tcaaaaactt caagaaaaaa ttgatcgtat gacaaaagaa aagatttaga
    26761 aggacaggtg gagtgaaaaa atgaagcgaa caaacttggc tgacaaagat actttcgctg
    26821 agcagaaagt attgattaaa gcctatctca aagttgtaaa agctaaagaa atatgtcgag
    26881 aagtgaattt atctaacaca acaatatctg agttgaagaa tggtcgaaag aatttagata
    26941 ccttaaaatt cagatcattt gaagctctat atgtagctgc taaaaaatat tttacagctg
    27001 ataacaaata aaaaacagga gtattttatg tgaaaaaagt atataaaaaa tgcttatgaa
    27061 tcagctatat caccttaacc atttgttaat gagatgggag aaaacattaa ccacgattct
    27121 aacgcaaaca tcaagcacat aaaacattta acactcctcc tttctggctg aagatttcaa
    27181 ccaaacagga agacagcgta tcaattatag catacacttt ttcttaaatt catttttcat
    27241 atgacaaaat atttccttct aatcgacaaa gttagaagga ttttttattg tctaaataca
    27301 caaataacct taatttttaa agagcataag gaaacgtgat atcactttct cacgaaaggt
    27361 gttaggtagg ctgtgcctac cacgtttcta agaaagataa atgatatcaa aattttggat
    27421 ttgtgtgtag atttgtgggt aggatgaaag cgaatgtggg cagtaaaata agcgtttgtg
    27481 tgtagcaaaa tcaaaaaatg tgtagggata tgtgtagcta cacatatccc tacacataat
    27541 aaatttggag gaaaatatgg acttattaat tagaaatatt gaagaaaagt atattaataa
    27601 aattgacaaa cgttgtgaag aacttacggt caagactggc aaaaaatgga gtcgaaatca
    27661 atacctaaaa gttttgatcg aaaatgattt tgatcacgct cttctgaatt ataaaaaaga
    27721 ccaatttgat cggttgttag aaaaatttgt ggatattcaa acttataata cgaaaacttt
    27781 agaagaatat attgcaacaa ataatcaatt aattggtttg ttaattgaat aataaaaata
    27841 gtattgtgaa tttataaaag gaggaaatca attttggcaa aagcaacaag aaaacatgta
    27901 tatctttacg atatgcatat cgaagtactt gatgaaataa tttctactca tgaagatata
    27961 tctaattata gtgaagcagt cagacatttg tgtatgatgt atgatccaaa aatcgaagaa
    28021 aaaataaatc caattcaaat taaattgaat gcaatgggaa aagaaatttc tattttaacc
    28081 gaaatggttt cagagtttgc ggatcttcat ttgaaagata caggaatact aattggtaca
    28141 aattcaactg tttatcaaga agcagaagaa cgtgtggaag gaaaaattaa atccaatcaa
    28201 accaaaaaat attcttctaa gttaaagaaa ccagtgaatg aatctgttca taccagagta
    28261 gatccatttg atccaacgaa gattctaaaa agataaaagt agaaggagaa cgtgatgaat
    28321 aaatcgcctg cagttattct tacttgtcaa tttaccttgc ccaataaaaa atctttttca
    28381 acatacattg attatatgac aagagaaaaa gcactggaag aaatggaacg acgtacgccc
    28441 gaagaagaac aggaactgca acgaatcaag aatgctttag aggattttta tattcctgaa
    28501 ggagaaactt tttcggaaag taagacggat aaagaattca gtgaaacgtc gaatgaagct
    28561 caatcgttga tggaacatgg aatcaatttt gacaagttag aggaacaaga ttttaccaaa
    28621 tatctttctt atatgacccg tcactatgcg ttagaacaaa agaaagaact gacgcaaagg
    28681 gaacaaaaag aataccaacg gttgaaccaa gcaatgaaaa aatatcaaac agagccacat
    28741 aaaaaagctt caggaaatct tcctggagtt ttttctatgg cttcagacga agtgaaagga
    28801 agtgatctga aggagatcaa acaaatcttc caaagaggac aacaaaaagg ctcaatcatt
    28861 tatcaagatg ttatctctca cgacaattcg tatcttgaaa aaatgggctt gtacgatccc
    28921 aaaacagata ccttagatga agaaggtttg aaagcagctg gaagaaaaat gatggagaca
    28981 ttattcgaga aagaacaatt gaatgaaaca ggttactgga tggcaacgat tcaccgtaac
    29041 actaagcata tccatattca ttttgcagtt gttgaaaaag aaaatacgcg taaaccgtat
    29101 actgagattg aaaatggtgt gagttatatc gtaccgaagg ggaagagaag acaagcaaca
    29161 ttagacgcaa tgaaaaccaa ttatgtacat gagttagaac gtttctctta tgaaaaggat
    29221 cgaatgatcg gtatggagaa acgaaattta ttgacacgaa aaagtgattt gcgtaacacc
    29281 ttaacaaaag tagtaaaaga accagcacgt tacgatcgac acgccatgaa cttattaaat
    29341 gaagtatata aaaccctacc tgaaaaacgt tcggaatgga attatggaga tgaaaatcga
    29401 accaagcttt caccatttac aaggggaaaa cttgatgagt taaccaaata tgttttaaca
    29461 aaagaacctg gatatcaaga atatatacag atttcaaaag cattgaaaga agaagccaaa
    29521 agcctttatg gggaaagcaa aagagaaaca aagaatgcag aaaaaaacgc cctctttgat
    29581 ttaaaaaagc gcacaggaaa tgcgatttta acagaactga agagacagga tagtcaaata
    29641 aaaccattaa tagaaagtgt caatcagatg caatctattt acaatgaacg atcctttcga
    29701 acactcaaac agttgccaac aaatgaatac aagtatagtt atcaagaatt cgtaaagcgt
    29761 aggaaacaat tccaacaaag agttgtcacg cagcgaagct tgcgtcaatt aaatcgaaca
    29821 attaatgcac atgaagaaaa ataccacgca agtaaagcat atgaagagca acagcgaaga
    29881 atccaaatgg aacaagaaag acaataggag gaagagaaat gaaacaatat ttagatcaat
    29941 ggaaagtcat agaaggatca ctaaaagaag aacgaatcga acaacttcct gattgtttag
    30001 aaaaagagca tttgtttcag ataagcgaaa tgttgagaaa cgaacagttt gatccaaatc
    30061 aatttttagt tgttgagtac ccagctactg gtgtgtattg ttgtaatcat gtgaagggtg
    30121 aaaaatattt tattattcaa gaatacgaag gtaagttagc gccttattat actacttggg
    30181 aaataaatga agagggaatc aataatttcc catgtgaaag cattgaagag tctatttctc
    30241 tcacggaatg ttaaataaat agagacgtaa aagagtcctg aacgtgattg tccaggactc
    30301 tttttcaatg aattgttttc cgccaattca ctgagtggtg cgattcaatt ttattatatc
    30361 aaaagaaagt gagattccaa agtgaaggga ggaagaagaa atgactcaga caacgattgg
    30421 tgttgtggtt gaaaaaagca gggataatct cgtttttgtg acagaaattc aaacaggtag
    30481 agcttttgta gtcacagaca aagctgcaaa agcgtatcaa aatggcaata ttttaacgtt
    30541 aaatctgact acgaagatgt tcgttgatgc agtagaaaat tatccatttg tttagacaaa
    30601 cgatatttta caaggaggaa acggacatgg cagaagaaaa agaaccacta gataatagtc
    30661 ggttgggaaa gtccaaaaga aaattagtac ggttgcaaaa cgaattaaat aatcaatttc
    30721 aaaagctatt tgatcaccaa aaattgacaa atgggcaacc aatgaatgac aaacgaaatg
    30781 gtcgttcatg gtttaaccag caagaaaaaa tagaaaataa aatacaaagt ttgagagaag
    30841 agatcaagca acaagaaaaa caggtagaaa aacttgaaag acaagaagag ctaaaggaaa
    30901 tgggctataa caagtacggc ggactggata tgacgatcga taatattcca agaatcaaag
    30961 aagaaatcga acgctttgaa aaaggtgaat cgactttttc agccgccacg atccgaaaat
    31021 atcaacggaa gttggaaaca ttagaacagc tgaaagaaaa atcagaaaaa ggaaaagaaa
    31081 atatcctccc tgaagtacaa gcgatcattg acagcggacg agtgacgcaa tggaaaaaaa
    31141 atccaaccat ttattttttg aaaggctatc gaaaagttgc tttagaactg aatgaaaacg
    31201 ggggatttga ggaatcaaaa aaataccaag ccacgtcgga agaggaaaaa gcagctgttc
    31261 gtgaattact tgaagaggct gcaccaactg attctctgaa ggagaaagaa aaagatcatt
    31321 tctatcgagt agaattcaac gaaaatgatc cagaaaaagg attgaaaagt tatggtgggg
    31381 agatcgtaac attagaattg atcgatcaac ttaaaacgtt ggataaccaa tacgctgcag
    31441 aagcaggtta ttataaattc tattttgaag aagtgaagaa cggagaagta gtagatcaga
    31501 tccgaatgga tctaggggat ggcgtcaaca gtaatcaccc catctattta gagttagcaa
    31561 aattgtgcga acaagtacct gagaaggaac aaaaacaatc ggtcaaaaat gaacctgttt
    31621 cgaaagaaca aatgcagtcg aaagaaacaa aaacaaaaca aaagggctat caaaaacgaa
    31681 cggcagaaga gatcaaacaa gaagttaaat ccctttcgca acaggcactg gatgcggtga
    31741 aaaagcatac ccattcttct gaagacgtca aagaactttt gaactttatg tctcgttttc
    31801 cagaacgatc ctttagaaat caattattga ttgaggaaca atttccaggt gcaacggctt
    31861 gtttaggtcg tgccgcgttg aaaaaagaag gaatctatat caaaaaaggg gaacaaggga
    31921 acaagatctt tgtcagaaaa acggtcaaag gattttatga gaaaggacgt ggctttgttc
    31981 gagaaacaga agctacacca gaagataaaa aacgaatcga aagtggtcaa atcgaagtga
    32041 tcaaaaaa
//
LOCUS       DAJSKS010000014        31455 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJSKS010000014 DAJSKS010000000
VERSION     DAJSKS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.014"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            212..604
                     /locus_tag="ON396_000606"
     CDS             212..604
                     /locus_tag="ON396_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3112737.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ON396_000607"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ON396_000607"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCR3112738.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="ON396_000608"
     CDS             3165..4775
                     /locus_tag="ON396_000608"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCR3112739.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="ON396_000609"
     CDS             5067..5936
                     /locus_tag="ON396_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCR3112740.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ON396_000610"
     CDS             6101..7372
                     /locus_tag="ON396_000610"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR3112741.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ON396_000611"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ON396_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR3112742.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ON396_000612"
     CDS             8763..9026
                     /locus_tag="ON396_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCR3112743.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ON396_000613"
     CDS             9471..9920
                     /locus_tag="ON396_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3112744.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ON396_000614"
     CDS             9977..11395
                     /locus_tag="ON396_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3112745.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ON396_000615"
     CDS             11395..12255
                     /locus_tag="ON396_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR3112746.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ON396_000616"
     CDS             12429..13202
                     /locus_tag="ON396_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3112747.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ON396_000617"
     CDS             13349..13714
                     /locus_tag="ON396_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HCR3112748.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ON396_000618"
     CDS             complement(13862..15070)
                     /locus_tag="ON396_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3112749.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ON396_000619"
     CDS             complement(15089..15367)
                     /locus_tag="ON396_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112750.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="ON396_000620"
     CDS             15521..16216
                     /locus_tag="ON396_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR3112751.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="ON396_000621"
     CDS             complement(16658..17455)
                     /locus_tag="ON396_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCR3112752.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ON396_000622"
     CDS             complement(17452..18636)
                     /locus_tag="ON396_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCR3112753.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ON396_000623"
     CDS             complement(18661..19908)
                     /locus_tag="ON396_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCR3112754.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ON396_000624"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ON396_000624"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR3112755.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ON396_000625"
     CDS             21888..22202
                     /locus_tag="ON396_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR3112756.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ON396_000626"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ON396_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR3112757.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="ON396_000627"
     CDS             22887..23531
                     /locus_tag="ON396_000627"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR3112758.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ON396_000628"
     CDS             23546..23875
                     /locus_tag="ON396_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCR3112759.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ON396_000629"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ON396_000629"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR3112760.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ON396_000630"
     CDS             24863..25687
                     /locus_tag="ON396_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCR3112761.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ON396_000631"
     CDS             25680..26027
                     /locus_tag="ON396_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCR3112762.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ON396_000632"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ON396_000632"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR3112763.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ON396_000633"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ON396_000633"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR3112764.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ON396_000634"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ON396_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCR3112765.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ON396_000635"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ON396_000635"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR3112766.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
BASE COUNT        10111 a         5635 c         6634 g         9075 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgt
//
LOCUS       DAJSKS010000015        31351 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJSKS010000015 DAJSKS010000000
VERSION     DAJSKS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.015"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(125..1228)
                     /locus_tag="ON396_000636"
     CDS             complement(125..1228)
                     /locus_tag="ON396_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCR3112767.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(1450..1920)
                     /locus_tag="ON396_000637"
     CDS             complement(1450..1920)
                     /locus_tag="ON396_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HCR3112768.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2177..3496
                     /locus_tag="ON396_000638"
     CDS             2177..3496
                     /locus_tag="ON396_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HCR3112769.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            3701..3988
                     /locus_tag="ON396_000639"
                     /pseudo
     CDS             3701..3988
                     /locus_tag="ON396_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4047..4487
                     /locus_tag="ON396_000640"
     CDS             4047..4487
                     /locus_tag="ON396_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112770.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            4499..5167
                     /locus_tag="ON396_000641"
     CDS             4499..5167
                     /locus_tag="ON396_000641"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR3112771.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            5231..5854
                     /locus_tag="ON396_000642"
     CDS             5231..5854
                     /locus_tag="ON396_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HCR3112772.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            5910..6296
                     /locus_tag="ON396_000643"
     CDS             5910..6296
                     /locus_tag="ON396_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HCR3112773.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            6509..7900
                     /locus_tag="ON396_000644"
     CDS             6509..7900
                     /locus_tag="ON396_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCR3112774.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            8511..8879
                     /locus_tag="ON396_000645"
     CDS             8511..8879
                     /locus_tag="ON396_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR3112775.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            9059..10000
                     /locus_tag="ON396_000646"
     CDS             9059..10000
                     /locus_tag="ON396_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR3112776.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            10069..10800
                     /locus_tag="ON396_000647"
     CDS             10069..10800
                     /locus_tag="ON396_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCR3112777.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            10944..13124
                     /locus_tag="ON396_000648"
     CDS             10944..13124
                     /locus_tag="ON396_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR3112778.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            13144..13626
                     /locus_tag="ON396_000649"
     CDS             13144..13626
                     /locus_tag="ON396_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCR3112779.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            13642..14403
                     /locus_tag="ON396_000650"
     CDS             13642..14403
                     /locus_tag="ON396_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR3112780.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            14534..15868
                     /locus_tag="ON396_000651"
     CDS             14534..15868
                     /locus_tag="ON396_000651"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR3112781.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            16175..16660
                     /locus_tag="ON396_000652"
     CDS             16175..16660
                     /locus_tag="ON396_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HCR3112782.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            16653..17336
                     /locus_tag="ON396_000653"
     CDS             16653..17336
                     /locus_tag="ON396_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR3112783.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            17462..20107
                     /gene="polA"
                     /locus_tag="ON396_000654"
     CDS             17462..20107
                     /gene="polA"
                     /locus_tag="ON396_000654"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR3112784.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            20152..20988
                     /gene="mutM"
                     /locus_tag="ON396_000655"
     CDS             20152..20988
                     /gene="mutM"
                     /locus_tag="ON396_000655"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HCR3112785.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            20952..21581
                     /locus_tag="ON396_000656"
     CDS             20952..21581
                     /locus_tag="ON396_000656"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR3112786.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            21633..21965
                     /locus_tag="ON396_000657"
     CDS             21633..21965
                     /locus_tag="ON396_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR3112787.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            22277..22768
                     /gene="nrdR"
                     /locus_tag="ON396_000658"
     CDS             22277..22768
                     /gene="nrdR"
                     /locus_tag="ON396_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCR3112788.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            22791..24164
                     /locus_tag="ON396_000659"
     CDS             22791..24164
                     /locus_tag="ON396_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCR3112789.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            24164..25090
                     /gene="dnaI"
                     /locus_tag="ON396_000660"
     CDS             24164..25090
                     /gene="dnaI"
                     /locus_tag="ON396_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCR3112790.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            25219..25659
                     /locus_tag="ON396_000661"
     CDS             25219..25659
                     /locus_tag="ON396_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR3112791.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            25803..26504
                     /locus_tag="ON396_000662"
     CDS             25803..26504
                     /locus_tag="ON396_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCR3112792.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            26501..27622
                     /locus_tag="ON396_000663"
     CDS             26501..27622
                     /locus_tag="ON396_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCR3112793.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            27637..28869
                     /gene="pepT"
                     /locus_tag="ON396_000664"
     CDS             27637..28869
                     /gene="pepT"
                     /locus_tag="ON396_000664"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR3112794.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            29001..29909
                     /locus_tag="ON396_000665"
     CDS             29001..29909
                     /locus_tag="ON396_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HCR3112795.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            29944..30216
                     /locus_tag="ON396_000666"
     CDS             29944..30216
                     /locus_tag="ON396_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HCR3112796.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            30227..31273
                     /locus_tag="ON396_000667"
     CDS             30227..31273
                     /locus_tag="ON396_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HCR3112797.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT        10381 a         5579 c         6663 g         8728 t
ORIGIN      
        1 gtatgaaaaa atatttcaat tttatttttt cgtttcaaat tattctatta gtgacatcct
       61 tgaactataa aaataggagt acggcatcat ctgccgcact cctatttttt tcgtcatttg
      121 tttcttaaat aaaacgattc tcacgcaatt ttattttttt gatatcttct actgtttgtg
      181 ttccagctaa ttgcatgacc atttccaatt ctgttttaaa gaaatcaaac acttgatgga
      241 ctcctgtact tcctcctaaa gacaatccat aaataacggg tctgccaata gcaacaagat
      301 ctgcacctga tgcgattgct ttgaaaacat gctgtccgcg tcgtacaccg ctgtcaaaca
      361 cgattggtac acgtttatct actgcttctg ccacatattg caacgaatca aacgctgctg
      421 gaccaccatc tagttgacgg ccaccatgat tagataccca gattccgcca gcaccagatt
      481 ctaatgaacg atagacatct tcttctgact ggacaccttt cacatagact ggcaaatcag
      541 aatgagcagc aataaattct acatcttttg gactaagctt ctgtttggaa gatttgtaca
      601 cagcatccat cgtttggcct actcctgatt gataagcctg aacgatcggc attggcagtg
      661 ggaaggtaaa cccatttctg cggtctgttt cacggtttcc gccaactgtt gcatccgctg
      721 tcaatacgat tgcttttgct ccattacgct tggccacttc aagaatatcc aaattgatcc
      781 catcatcttt gctcatataa aattgaaacc attgcggtgc tttctccccg cctgcttctc
      841 ttatctcttc taatgtacaa gaagcgtaag aacttgctgt atagatcgtc ccaaaatctg
      901 ctacaccttt tgcagaagct ttctccgcct tcacatgagc taagccatga gcagcaactg
      961 gtgccatgat gattggtgca gtcagcgttt ctttgtcaaa atgtgtagtt gtatcgggta
     1021 attctacatc tcttaataca tgaggaataa tcagctgatg attgaatgcc cgttcatttt
     1081 cttgataagt aaataagtcg ccagcaccac tgctgatgta accataacca ccagctggaa
     1141 tcacttgtga agcggctatt tctaaatctt ccatattgat aaaatccact attccttcat
     1201 ttgtacctgc ttgatacgtt ttttccattt caatgccttc cctctataaa aaattgaatg
     1261 aataatatga tagcgtttac acactttata ttactcttcc gtattgatac aggcaaataa
     1321 tctgtttaca tttcagtcat tccagttgtt actactctga aagaaatgtt tagaacacaa
     1381 atagataaaa aacgatggga ttttgacaaa agtcgtgtca aagtcccttt tacgaaatac
     1441 tgtggatgcc tattgaaata agtgcgggtt gtttctttgt atttcaatcg tttcttgcca
     1501 ttcttcgttt aattttggat taccgttttc ttccttgaac gcataaggtc ccaatccaaa
     1561 aagctcagtt agtatttgat tcacgattcc atatagctcg gtgtgatcgg acacatgatt
     1621 attgaagatc atctcattat caccacgttt gaatctcaca ttcgggctct catgatgaac
     1681 ctctatgcag ttttcccctt tttttaactg acggatttct gaaaacatct cataaatatc
     1741 ttctccaata aaaccttcag caagcgccag actgtccaca agaccgccta ctgtcaactc
     1801 agagattatc gctttgttgg caccaagctt taatgtatag cttgttgctt ctactacgcc
     1861 gcatgcattg caaccacctt ctgatagttc gctttttctg atcaattgaa cttctttcat
     1921 tttctgttcc tcctcttagt tttctactta tgtatttttt atcacctgac gtaagtgtat
     1981 tttatcgtca cgatcacgtg atttattctt cagagattct acctgaataa ttcatacttt
     2041 taattaaaac catgtgaaac tgcctcacgg cagcttacaa ttactttttc atcttcaccc
     2101 gttaagtgat gaaaaaagaa cccctttctg ctatggttaa agtgactaaa ccaaccaaaa
     2161 gaaaggagtt cttttcatga ctagcttaca caaaaaccaa gtaaaattca attcaaatat
     2221 cactatttct catacaggtg gtcaattatc gagtgattcg ggtctcgtcc tagtgaaaga
     2281 gctgatgaac atctttggtt tttctgaact ggctaagcaa cacattcaca ttgaagacga
     2341 acgagcgtat tttactcatg acaacttatc catacttgag cagttcatta tgcaattaat
     2401 tgctggttac tcagctgatt ctgcagctaa tctcctgaga caagatcctg tgtttaaagc
     2461 tgttttagat aggaaagaac tcgcttctca atcttcactt tcccgatttt tagatcgact
     2521 atctgaggag aatatccatg aacttcaagc tttgaaccaa gaacttatcg ataaagcacg
     2581 cctcatccgt aatgatacgg aattaatcat tgatttagat tcgacccatt cagatacatt
     2641 tggtcaccaa gaacaaacga attataatac acactaccaa acctatggtt accatccatt
     2701 ggtagctttt gacggattga ctggtgactt cttaaaagct gaactacgtt caggaaatca
     2761 atatacatca aaaggcgtaa aggagtttct cacaccttta ttagagcact ataatcactc
     2821 tctaccaaac actgacatct tggttcgtgg agacagcggg ttcgctacac ctggtgtgta
     2881 tgattcgtgt gaatcaaaaa agagtcatta tgttattcga ctgaagaata atcgtagact
     2941 aggtcaaata gctgagaaat cagtacttta tggcgataat caaaagtggg aagaacgaga
     3001 agttcagtac ttctcgacca cttatcaagc acaatcctgg tcacaaagtc gtcgcgtgtg
     3061 tatacgctca acacgtgaag cgggcgaact actctttcga catgaattta tcgtgacgaa
     3121 tctatcagaa aatgtttctc ctgataccat cttttctatc tatgccaaac gtggcacaat
     3181 ggagaacttc attaaagaag cgaaagctgg cttttacttt gacaaaacag acagtcctcg
     3241 ctttttggag aatcatgtcc gaatgatgct cagcttgata gcttacaact tagtcaactt
     3301 cttaagaacg attggctttg aagaagtcca aaagggaatg accattcatt ctattcgatt
     3361 gaagtttctg aaagttgctg ggaaattagt ccaaacgggt agacgagtct atctcaaatt
     3421 atctagttat catgtgtatc agaatgaatt ctacagggtc tttgctcgcc tgaggcgagc
     3481 cagtcaatgg atctaatcca acaatctaac gattttttta ctgggaagtt atccagggag
     3541 aagtatgctc aaaatgcctt acacccaaaa tttattcctc aatatttttg aacagtgaat
     3601 gtttgaagta actttttagt caaaatcagt tgatagggat attagtgata catatttcaa
     3661 taaaatgtgc caaagaatta aagctatgaa ttattcaggt ctaaaaagct ttttaaaagt
     3721 gatcagtaaa aaacggaatc tgatcaaaac gacacagctg ttgaaaaaat atccaggtct
     3781 gcttcacacg tctattcaat ttcctcaatt gattacgatg atccatcgtt ttatacgtat
     3841 gcaacagcta ccatctaaga agctgtttct tccattgacg gacacatttc gagtagtcaa
     3901 cagagaagat aaaaagaaag tgattgtgat tgactttacc agaaatcgga atgtccttca
     3961 ccaagttgca atggaaaagg ccaattaagg ctttttcttt ttggattttt ttgtcataat
     4021 aaatattaga tgatggagga gataatatga tgcaaacatg gttagaaagg ctaatacgat
     4081 ttcagcagaa ctatcacaaa cttatgcagg gaagatatgc gaaatttgat cagttgaatc
     4141 gcggtctatt gatcgctgcg cttgtcttta gtttgctaag tcgttggttg ccctatagaa
     4201 tcggacaatt gttgttcatc gtctttttcg gatggatgat atttcgtttc ctatcaaaaa
     4261 aaatctaccc cagactaaat gagaatcaac gttatttgaa atatcttgat agagtcaaag
     4321 aaaaatatca tcagataaaa aacaaaaagc aagtcaaaat ggatcgaaaa agttatactt
     4381 tttttgagtg cccgaattgt catcaaaagc agcgtgcacc taaaggaaaa ggacgaatac
     4441 gtgtgacatg caaaacatgt ggtataaaat tcgaaacgaa tgtttaaggg agaaaagaat
     4501 gaaattagta ttattacgtc atggcgaaag cgaagcaaat tttgaaaatt actggacagg
     4561 atggctagat gtcgccttaa cagaaaaagg aaaagaacaa gcagcagttg ccggtcaaaa
     4621 aatgcttgaa gccggacttc aattcgatgc cgtttatacc tctgttttga aacgtgcgat
     4681 tcttaccgga caagtcgcac tagaagaaat gggtcagtta tggcttccta taataaaaac
     4741 atggcgtcta aatgaacggc attatgggga tcttgtgggt aaaaacaaag ataagatgaa
     4801 agaaatatac ggaaaagatc aagtaaaaac atggcgcaga ggatatacgg aagttccgcc
     4861 gttaaccgca gaaaatagat ttgaccgcag atatgatcac ttggatccgc atttgatccc
     4921 atacggagaa agtctggaaa tgacagtgaa tcggatcatt cccttatggc aagatcatct
     4981 agcaccaaaa ctaaaagatc ggcaagattt actagttgtg ggtcatggta atagcatccg
     5041 agcactgaca aaatatttag aagatgttcc tgaagatcaa atggatacga tcgatatccc
     5101 aaatgcacaa ccaatccagt atacattagc agatgatttg acaattctgg acaaaaaaat
     5161 actttaaaga acacctttat ctaaaagtga gagatagaaa aaagaagcaa aggaatggaa
     5221 caaaaggaac atgaaaaatt atatatggga ctttgatggt acattgtttg atacgtatcc
     5281 cgcaatggta gatggtgcat ggcaagcact aaaagatttt ggtatcagta tggacaaaaa
     5341 agaaatctac ttcaaaatga aaaagtattc tactagttat ttgatcaatg aatcaaactt
     5401 gaatgccaga gagtttaatg aattatttca ccgatatgaa aaagaaagta cggaagtttc
     5461 ccgtccattt ccagaaacga aacaagtact ggaaatgcta aaagacaacg gcggtcgtca
     5521 tttcatcctg acccaccgtt tgacagaatc aacatggggc ctgttaaaag aacatcgatt
     5581 ggcacatctg attgaggaag tagtagggat cgatcaggac tttcctcgta aacctgatcc
     5641 agcatcttta aattatttga tcgatacttt tcatttagaa agaacagaca cgatgatgat
     5701 cggcgatcgt cgtttagata tcgaagcagg gaaaaatgca ggagtcgcta cttgtcttta
     5761 cgacatcgat cattttctgg gtgagatccc ggcagattat gtggtaggaa atctgaatga
     5821 aatccttacc ttgcagagca tcattaaaaa gtagtatccc ctttcaaagg agatatgtta
     5881 taattttcat aaaggagtga taaaattata tggaaatcag aagacaaaaa gaagttgtag
     5941 aagcatttca ttatgatatg agattgccga accaagaagt agaaacagat ttgaaagtag
     6001 gcttctcgcc tttaaaagaa acgatcgaaa actatccgaa agaacatagc atcgtaggag
     6061 cacgcttaga gttccgttta gtgtttgata catatgtttt atctggaagt gtgagtcaga
     6121 tcaatcatat cctcaatcgg aaaattgaag gtcaaaatga tgtcacacaa gaagaagtgg
     6181 acgagctagt agcaccgtta ttcgatatcg tacgccgaat gtcttatgag gtgacagaga
     6241 tcgccttaga tcagcctggg gtaaaattga attttcaatc agaaaaacaa gaatagacag
     6301 taaaaacata cctgcgatag attaaagcta tcacaggtat gtttttttat tactaattgt
     6361 aagcaaaaag aatgtcattt cgataaaaat cgtctaaaat ataaaagtca ctgaaaaaaa
     6421 ttttttcagg aggatggttg aagaatcatt ctcgggaacc tttacttcct tcgatggact
     6481 tgtcattaat aatatgagga ggagaaaaat gacaacactc atcacagttt tagtggttct
     6541 agtttttgcg gcattattat atgtgttcta tcgtatgcaa aagcgccata caaaattttc
     6601 aacacgcgtc tttacagcat taggagttgg tatcgtcttt ggcggtctgc ttcaattgat
     6661 ctttggtgta gacgacaaag tcgttactca atcaatggaa tggatcggga ttgtaggtca
     6721 aggatatgta tcattcttgc aaatgctagt tatgccttta gtgtttattt cgatcattgg
     6781 tgcattcaca aaaatgaaag tttctgaaaa gctagggaaa attagtgcga ctgtattgac
     6841 ttctttactt ggaacaacag ctattgcagc attgatcgga attggttcaa caatgctgtt
     6901 cggactccaa ggagctaaat tcacacaagg agctgcagaa acagcacgaa tctctgaact
     6961 ggcaacacgt caggaaacag ttgaaaactt atcgattcca cagcagattt tatcctttat
     7021 ccctaaaaac gtttttgctg atttagcagg tagtcgtgca acaagtacga ttggagtcgt
     7081 tattttcgcg gcatttgtcg gaatcgctta tctaggcgtg aaaagaaaag cgccaaaaga
     7141 agcagcattc ttttcagatt tagtcgacag tctgtataaa atcattatgc ggatcgtgac
     7201 gcttgttttg cgtttgacac cttatggagt atttgctttg atgacaaacg ttttggcaac
     7261 aagtgatttt gcagctatcg tcaatttagg taaatttgtc gttgcttctt acgtagcatt
     7321 gcttgctgtt ttgattgtcc actcactgat tttgattggt gcaaaagtca atccaatcaa
     7381 ctatttcaaa aaagcctttc cggtattgag ctttgcgttt acttcacgtt caagtgctgg
     7441 agctttgcca ttgaatatcg agactcagac aaaagcttta ggagtagatg atgcaacagc
     7501 taatttctca gcaagttttg gtttgtctat tggacaaaac ggctgtgctg gcgtttatcc
     7561 agctatgttg gcaacgatcg tcgcaccagc agctggagtg aatgtcttta gtttggaatt
     7621 tattttgatg cttgtggcaa ttgtaacaat cagttctttt ggtgtagctg gagtcggtgg
     7681 gggcgcaaca tttgcttcac tgatcgtttt aggggcaatg aacttgccag tagcaatcgt
     7741 tgggttagtt atctctgttg aaccattaat cgatatggca cgtacagcag taaacgtcag
     7801 cggtagcatg gtagctggta tcgtgacaag tgcacgcatc aaagatttag atcgcgatgt
     7861 catcaatgat gattctaaag tgatcgatgt gaatgcatag tcaaaaagag gttgtgatag
     7921 aagtggtcag ctttaagaaa taagaagaag tttcctaaaa atagtttctc aaatttttgt
     7981 gaaatttcgg cttatttccg agaagctact tctgaaacac cgtgaataag aggttgtgaa
     8041 aaagctgccc tgcatcaagt aataagaaca gccaaacaaa aatagttcct catattttac
     8101 gtttggttga gcttattacc gcagatgcaa gcttttgaac accgtttaag aaagaggtcg
     8161 tgagcctgcc gtttcactct gagtcacaaa gagcaatttc aaaaaatagc ttctcatgtt
     8221 tgttgaatat tgtgtcttgt gaccgaggag ttgtcaggag aacaccgttt accagaggtt
     8281 gtgacttgtt tttagtcacg acctcttttt ttatttattc catataaata cacattattt
     8341 attccgaaaa gtgaaagcgc aaataacaaa aacaaccaac ttatttatta ttaaagaaaa
     8401 gcgtatttaa gtaggacgat tctgttgaga gctttcgtag attttcccat tttcctatgg
     8461 taaactgaca aagttgagaa tgttagaaca gatcgtttag gaggaaacag atgtttacga
     8521 atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg ttttggagag
     8581 cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata attgaagtag
     8641 gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt gagtcccatt
     8701 cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat atctttggac
     8761 tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag cttggagaag
     8821 aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca ggtaaataat
     8881 agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt taattcaaat
     8941 aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca aataaatcgt
     9001 caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga agaaatacat
     9061 ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag aggaatttgc
     9121 caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc tagtagctga
     9181 agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct atatgaaagc
     9241 aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa gcgaaggaca
     9301 tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa agctagaaga
     9361 ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat ttaaagaagc
     9421 tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat atgacttggg
     9481 acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc cagactggat
     9541 ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta ctggtggcat
     9601 tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg tcggacagct
     9661 gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat tatgggacgg
     9721 caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag ataaacaaat
     9781 cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt gtagcaatcc
     9841 tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt taggagcatg
     9901 tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg gaatctctcc
     9961 agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag ttttgattgt
    10021 cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat gaaatcattg
    10081 atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact tattcactat
    10141 tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg aggtcctcgt
    10201 acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc catttacctc
    10261 cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga ctggtatgcg
    10321 ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc aggggcatcg
    10381 gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc agaactgatt
    10441 aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt tgtccatgcc
    10501 ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt tatttggata
    10561 agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt tggacatacg
    10621 attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca ggatcataaa
    10681 atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt ttttgacaag
    10741 gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca tggaatctga
    10801 ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt ttatagagaa
    10861 tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa agaagcaatt
    10921 tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc attcagcttg
    10981 tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt aatttgttaa
    11041 atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact ggaagtcttg
    11101 atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac ttagaaaaac
    11161 gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc gagttagaag
    11221 cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt ccatacaaac
    11281 aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca ctcgcgaagt
    11341 ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat attgacaaag
    11401 aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt attgctgaac
    11461 aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat aacaaaggta
    11521 tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat gaaatgtatt
    11581 atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct actaatcgtg
    11641 gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca attttagctt
    11701 acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc gtcagagaag
    11761 cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa ctgcgcaatg
    11821 aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac ctccgtaatc
    11881 ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg gcttatcgta
    11941 ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc gaagttattt
    12001 atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct tttatccaat
    12061 taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc agtcgggaat
    12121 ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat tacgctatcg
    12181 tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa gaatttccgc
    12241 atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa gatcctttag
    12301 ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa catgacgttt
    12361 ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt aaccaagtag
    12421 gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga ttgaataaaa
    12481 caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc gcacgaactc
    12541 aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc ggattcttgc
    12601 gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa agttattcta
    12661 ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt acagaagaag
    12721 cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta tcagtcggag
    12781 aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat atgcgtgatg
    12841 agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg aaaccaggga
    12901 tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt gatatcggtg
    12961 tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc aagcatccta
    13021 cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc gatacgaaaa
    13081 aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga ggagtaacaa
    13141 aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa aagcttccag
    13201 cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg cgggcgctat
    13261 catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc agacgaagag
    13321 acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct agctggaaga
    13381 gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg agggttgcgc
    13441 tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg ccaagattgt
    13501 tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg tggaagatgt
    13561 catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc acaaaaaatc
    13621 agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa agactggaat
    13681 cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact gacggcacct
    13741 acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc acaaatgtat
    13801 ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc gactagaatc
    13861 ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag tgaaagtgaa
    13921 atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt tctgatagaa
    13981 tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct tcattcctta
    14041 ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg caggactcga
    14101 gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc tgtgccattt
    14161 gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga actgaatcat
    14221 caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg tctccatgac
    14281 gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga tatatcagag
    14341 gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt ttctcgtact
    14401 tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat tttcaatcgt
    14461 ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg ctagataaga
    14521 gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta tcaaaggttc
    14581 aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc aaggatcaga
    14641 cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca cgattctgcc
    14701 atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg catttcctga
    14761 ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc gctatcacga
    14821 ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct ctcacggaaa
    14881 aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa caagttatct
    14941 aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat ttgaggcctg
    15001 tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga ccaatatcga
    15061 ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt tccaaacaat
    15121 ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt tacgcaaatt
    15181 aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata tccaagcacg
    15241 aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg atgaatttgt
    15301 tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt taggtgttat
    15361 tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa tgctgacttt
    15421 cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg tagtcgatga
    15481 ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc aaaaatatcc
    15541 agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga ttgcattgat
    15601 ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg atatttttgg
    15661 ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga aaatcaaaaa
    15721 aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc atgatgcagt
    15781 agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg aaaaactttt
    15841 aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta taaaaataga
    15901 aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat gataaacggt
    15961 gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg agaagctgtt
    16021 ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct ctgtaaacta
    16081 taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt ggtacaataa
    16141 tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc cgagaaaact
    16201 aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag aatctattga
    16261 agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt tgtatatcca
    16321 acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag tgatgctgat
    16381 ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac atgttgtcaa
    16441 tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt ttcagtttga
    16501 ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag cggagaatac
    16561 gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag ttcctgaatt
    16621 ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca tcaatgtcag
    16681 gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt gcccttagtg
    16741 cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact tttatcaata
    16801 attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta atggttctag
    16861 gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc tactctcttt
    16921 tgaatggcat aacattagcg gttactcttg catactacga tattggaaca gtaacgcaag
    16981 ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta acaacaaaaa
    17041 gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg atcattgcac
    17101 tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc tccctactgt
    17161 tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt aatatctatg
    17221 tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg aagctgtatt
    17281 tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac aactaaaaaa
    17341 gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg ctttttcttt
    17401 tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag gagttaagaa
    17461 gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc gcgcattctt
    17521 tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata caaatgcaat
    17581 ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga ctcatgttct
    17641 ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg agtacaaagg
    17701 tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac gtgatttgtt
    17761 gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg atattatcgg
    17821 tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt caggtgatcg
    17881 ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa aaggtgtcag
    17941 cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt tgacgccaaa
    18001 acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc ccggtgtgac
    18061 taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag tcgaaggaat
    18121 ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga taaatgacaa
    18181 agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc ctgtggatgt
    18241 ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc cgttttataa
    18301 agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag aagtaaaaat
    18361 ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta ctacggatat
    18421 ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg tcggagtagc
    18481 gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg aaaaccgagc
    18541 gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg cgaaacgaac
    18601 ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg atgtcttgct
    18661 aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa tagctgctca
    18721 ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg cgaaaaaagg
    18781 tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag ctgcaatcaa
    18841 tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag atcttttccg
    18901 gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg ggatcaaagt
    18961 agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa gagagatcga
    19021 acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa agcaattagg
    19081 agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa ctggatattc
    19141 cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag aggatatcct
    19201 cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc tgaaagtgat
    19261 ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa caggacgatt
    19321 aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag ggagaaagat
    19381 acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg actattcaca
    19441 aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag ctgccttcct
    19501 agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg aaaaggcaga
    19561 ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta tcgtgtatgg
    19621 catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag cacagcaata
    19681 catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa gaatcgtacg
    19741 cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat atctgccaga
    19801 catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta tcaatacacc
    19861 aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg ataaacgatt
    19921 aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac ttgtcttcga
    19981 ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta tggaacaagc
    20041 tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt ggtacgaagc
    20101 aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa aatgccagaa
    20161 ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg aaaaacgatt
    20221 caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc catctttgag
    20281 gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt tttaattttt
    20341 catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata tcaatttacg
    20401 aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga ggatggaagc
    20461 caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga aaaaggagca
    20521 agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga tagcttctta
    20581 ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc attgctgcta
    20641 gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt atgggaagcg
    20701 aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga acaattacgt
    20761 ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac gattcgcagt
    20821 tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt ctatcagcaa
    20881 actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt ggcacaaagg
    20941 gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga tttgttttag
    21001 gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt cagtctcatg
    21061 gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct ggaacgcttg
    21121 ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat gggcaactag
    21181 atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa aaacttgatc
    21241 agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct ctaagttcca
    21301 atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat gaccactata
    21361 tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt ttgatgaaac
    21421 gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt ccaatcgaag
    21481 aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa aaacttgtgg
    21541 gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg tcaattttgc
    21601 tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat caaaaggata
    21661 aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa aatctaatcg
    21721 atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac caagcaagaa
    21781 tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat ttgactcaag
    21841 aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt gaccacattc
    21901 ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc gaagagaagt
    21961 tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt tcatctgctt
    22021 caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct cagtaatcaa
    22081 ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt acgcatgaaa
    22141 gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt ttgtgatttg
    22201 tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata aaatgcgatg
    22261 caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac gagtaatcga
    22321 cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg agaattgttc
    22381 gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa tcaagaaaaa
    22441 cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc gttcagcaga
    22501 aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg aaaaccgcgt
    22561 acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg tgatggaaga
    22621 tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc gccagttcaa
    22681 agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa aagaaaataa
    22741 taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc ttggaaaatg
    22801 cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct ccgctgagag
    22861 aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga gaagccttga
    22921 gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt cctgaaggga
    22981 tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat gatgcaagaa
    23041 aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt gatctgggac
    23101 ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc aaagactctg
    23161 ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag cttacccaat
    23221 tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa aaatttttag
    23281 atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc gaatcgctga
    23341 ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg acattcgatt
    23401 ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat gatccaatca
    23461 tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca cttggcgaac
    23521 tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa ctgcaaaaag
    23581 ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt gcggtttctg
    23641 aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt cagctgataa
    23701 aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca gaaaaaggcg
    23761 gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa tcaggtcttt
    23821 ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat aatgctagct
    23881 taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa atcgccacag
    23941 cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa tcaaaacaaa
    24001 agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag cttcctgatt
    24061 gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca gagattgacc
    24121 gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag acgtaggcaa
    24181 agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg aaatgatcgc
    24241 tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag aagaattgac
    24301 acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag aaaagcgtaa
    24361 atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc tgacgttgaa
    24421 ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac ggcaaagaca
    24481 agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc aatcggcttc
    24541 ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag ccctagattt
    24601 tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc tagtaggaag
    24661 ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag cgattgctgg
    24721 attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac aggcaatcgg
    24781 aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt tgatgatcga
    24841 tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg gtgttatcct
    24901 tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta caatggatga
    24961 attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa aagcgaaacg
    25021 gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc gcaaccgacg
    25081 caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa agcaggtcag
    25141 cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta atgcagaaac
    25201 ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt tggtctattt
    25261 tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga ttcgattgat
    25321 gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat atttgtcgga
    25381 acgattgaaa gtatcactca agtagaagca gaagaagaaa cgcttcagtt gaaactcgtc
    25441 gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa tgacggagtg
    25501 atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa aatcggtgac
    25561 aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc accgcaagtt
    25621 cctggagatt cgattattga tgtttcattg aacaaataac caataacatt ttttcaaaaa
    25681 taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata tgggaatttt
    25741 tttattccta gtctccttat tttatttgga aaatgctata attatctaga aggagtgaag
    25801 ctatgaacca tttagattta tctcagcgct tagccgcagt gggtgacttt gtccctgaat
    25861 ccgctcgttt agcagacatt ggatcagatc acgcttactt acccgttgca ttgatattaa
    25921 gaggaaaaat cgaatatgca gttgctggtg aagtcgttca aggtccttat gaatcagcac
    25981 gtaagcaagt caaaaaagat ggattagaag accgtatcac cgtacgcctt gcaaatggtc
    26041 tagatgctat ccagccacaa gatggtatta cagctgtcac gattgcgggt atgggaggtt
    26101 cactgatccg agatatccta gaagctggca agttgaatca acgcttaagc ggacaagaac
    26161 gtttgatcct tcagccaaac atcggagagc gtacattacg atcttggctg caagaaaatc
    26221 agtataaaat ccaatcagaa aaaatacttg aagaaaataa aaaaatatat gaaatcattg
    26281 tagccgaaaa agaacaaaaa ccagttactt acaatgaaat ggagctgatg ttcgggccat
    26341 ttctgcttga agagaaaagt gagatcttca aatcaaaatg gacaagagag cttcaacaaa
    26401 gaaaagctat ccgtgaacag ctcaaacaag cagctaatca agaggcacgt caaaaacaaa
    26461 tcgagcagga aatccaaatg attgaggagg ttttaaagcc atgacaatca gcggacgcac
    26521 tttcatccaa aagtttgaaa gctattgtcc tcagtggcta gctgaagaag gagacccagt
    26581 cggactgcat atcggaacat tggataagcc aatccaacga gtaatgatga cattagacgt
    26641 tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc gatttgctga ttgctaaaca
    26701 tcctccaatc tttcgtcctg taaaacggct ggttactgat cagccacaag aaaaaatgta
    26761 tgcagatttg ttgaaacatg atattgccgt ctatgcagca cacacgaaca tggatattat
    26821 ttgggatgga ctgaatgact ggttttgtga acttctcggt attgaagtgg agagttattt
    26881 agtaaagact catgagattc attataaaaa actggccgtt tatgtacctg tggatcatgc
    26941 acagaaaatg cgtgaagtat tagctgcagc tggtgctggt acacaaggtg attataccgg
    27001 tacaagtttt acctcgattg gacacggtcg atttactccg gaagctggtg cacaaccagc
    27061 gattgggaaa gtagggaaga cagaacaagt tcaagaggca aaagttgagg ttattttgcc
    27121 ggaaactatc gaaaaacaag tgattcaagc catgcggtca gctcatcctt atgaggaacc
    27181 tgcttatgat cttttcgcta tagatgaacc agtcgaaatg tttgggttag gacgagtagg
    27241 tgaattgccg caagaaattt ctatagaggc atttgtagaa caagtaaaag aagcgttcca
    27301 actagatgga ctgagaatag tccagccaaa aaatgcaaaa tcctctgtga agcgcattgc
    27361 gatttgcggt ggaagcggtg aaaaatttta tccgcaagca atcgcccaaa gagcagatgt
    27421 ctatatcacg ggagatatct attatcatac ggctcatgat atgcaaagcg ctgggttgat
    27481 tgcaattgat cccggtcatt atattgaatc gttatgcaag caacgattta tcgagaaatt
    27541 tgaatcttgg aaacaagaag aaaattggga tattaacttt ttcgtttctg aaacaaatac
    27601 aaacccattt caatttaatt aaaaggagag aatactatgt acgaaaatct tttgccacgt
    27661 tttttacgat atgtcaaaac agaaacacgc tccgatgcaa caagcaaaac gactccatcg
    27721 actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag aattagaaga gttaggctta
    27781 gctgatgttt tctataatga agataacgga ttcgttatgg gaacattacc aagcaatgtt
    27841 gatcatgaag tgcgttccat tggatttatt tcccacatgg atacagcaga ttttaatgca
    27901 gtaggagtca atccacaaat cattgaaaac tatgatggcg agtctacgat cgtactagat
    27961 gcggaagaac gctatacatt gaatacaaaa gattttccaa atttgaaaaa ttatgaaggc
    28021 cacacattga tcacaacaga tggcagtaca ttactgggag cagatgacaa atcagggatt
    28081 gcggaaatca tgactgcaat ggagatcttg atcaataatc cttctatccc tcacggtacc
    28141 atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg gagcggataa attcgatgtt
    28201 gagcaattcg atgtagattt tgcttataca gtcgatggtg gtcctttagg tgagttgcag
    28261 tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc aagggaaaaa tgtacatcca
    28321 gggacggcaa aagatacaat gatcaatgct ctacagttag caatcgattt tcataatcag
    28381 cttccagccg atgaagttcc agaaaaaacg gaaggatacg aaggtttctt ccatttgatg
    28441 gcattggatg ggaatcctga agaagcttct atgagttata tcattcgcga tcatgatcgt
    28501 gaaaaattcg aagcacgcaa agcacaaatc acacatatcc aagaagagtt gaatcagcga
    28561 tttgatcaag aacgaatcaa agtagatatg tatgatcaat attacaacat gaaagaaatc
    28621 attgaaaaag atatgagcat cgtggaatta gcgaaacaag caatgatcga gcttggtatc
    28681 tctccagtga tcgaaccagt tcgtggagga acagatggtt caaaaatctc ttacatgggt
    28741 attccgacgc caaatctatt tgccggagga gaaaatatgc acggacgatt cgaatttgtt
    28801 tctttacaag taatggaaaa agcaacagac gtgattgtaa aaattgcaga attgaacaca
    28861 aagagttaaa atcgaaaaag aacagcttat gacaaggcgt tcgtcccaat gatggaatgt
    28921 catctgtcta aggctaaagg gcaggacaat ggaattggtt gtcccgccct cttttttgaa
    28981 gagtaaatag gaggtaaaga atggaaaagc tgctagtttt ttataacaaa agttcaggga
    29041 aagatgaggg tgagaaatta gcacgctggt ttcaagatta tgtaaaagaa aagcagccag
    29101 aattatatgt agaactagct gaaactggcc catcgattga tgatgcggaa ttagtcaaga
    29161 aagcagaagc catagaagct gatactttag taattatagg tggagacggc acgattcatc
    29221 atatcgtcca agcgttccaa aaaacattat ctcaatacca tgtcggatta cttccaggtg
    29281 gaactgtcaa caaccttgca cgtgttcttg agattcccct caaaaaagaa gaagctgctg
    29341 acgtgatcct ggaaggcaat gtacggaaaa tagactatgg tcaagtcaat gatgaagtca
    29401 ttatctctac actgacaatc ggaatcttag cagatacagc agtttggatc agtcaagaag
    29461 aaaaacagcg ttacggctcg tggatcttta tcaagaagtt ttttaagtta ttgctgaaga
    29521 agaaaaaata tcatttggat ttagaaacag acgaggagca ttggaaagga aaatcccaac
    29581 tgttgacagt cacgatgtct aactccgtgg gcggttttac aaattttgac gactcagcga
    29641 ccctagatga tggaaaattt catgtaacga tcgtaccttc cttagatatt ttccgattta
    29701 ttttttattt gcctagaatc atgagaggca aattctatac gatccccggc attacttatt
    29761 tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc tgtgggcact cgaacggatg
    29821 gagacacgac agatgatctt ccagttgaat tatctgttgt atcaaaagga ttaacagttt
    29881 attcaccaaa gaaagaagaa aaagcttaga aatagtagtt aacagaaagt tggaagaaga
    29941 gtaatggatg tattgttaca caatcctttg tggttttacc ttttcattgt gaatttttac
    30001 ttattttgtc tgatggggta tgataaacat caggctagaa ctagcggctg gcgtgttcct
    30061 gagaataatt tgatatttgt cagcctgtta ggcggtggtt tgggaggatt gatctctcga
    30121 aaagttttcc atcataaaac tcggaaaaag aagtttacta taggattcgc tgcaggtgta
    30181 cttgttgatt tgattctcat ttattttttc cattaaggag caggagatgt cgtctaaagg
    30241 aacgaaaact aaaatcattc tgaatctgct tttgctgatt gtgatttttg ccattattta
    30301 ttatttaatc aatcaatcat ttgcagatat ctttgccgaa ttgctgtcta ccagtgttgt
    30361 tgttttggca gcggctgtta tattagggac agtttaccaa atcgctgaag ggcgctcaat
    30421 caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg aaagatgggt tcttgacctc
    30481 tagctatatt gcgttttacc gaatcgtttc gtttggtaca ggaacgctga tctcggagat
    30541 ttatttttat aacaaaaaag gaatgaagct atcccaggga gcaggggtga cagcactcca
    30601 tatgattatg tacaaactgg cagtgatcac ttatgcatta gctggcttgg tcatccaatt
    30661 ctctcttttt tattctaatg gtccatcaat gatctggttt atcatcgctg gtatcctttt
    30721 gacgatcgtg attgttgcct ttttattggc gatgtccgtc agcttgaatg ttcaaatatt
    30781 ttttgtcagc ttatcgaata agttatttaa aagtgagcgt ttgcggaaat gggtggatac
    30841 atgcaatcag cagatctact ctttaaggga aacggtccaa accattatcc aagacaggac
    30901 tgcgctattg cggatttatc tatggaatat gttaaaactt gtattttggt atgtacttcc
    30961 ttatttgatt ctgattgaaa accatcctaa aatagatttt ctactgacca tttcgtttat
    31021 cagctttgca gtagtgcttt caggggttat tcctacgcct gctggtattg gttcctttga
    31081 atttgtctat ttgttattat tcagaccagt tgttggaaca gttgacgcag tttcttcgct
    31141 tttactgtat cgtttcagtt cctttatctt gccatttctc tatggatttg tgtatgtgat
    31201 tgctgaacgg cgaagagtga tcaaagaaga aataatagaa gtaaaaaaag agaataaaga
    31261 aaatcaaggg taggtctttt ataagatcta ccctttttat caaatcaaag aaaaacgtta
    31321 tggataagat aattttacgt ttgtttggct c
//
LOCUS       DAJSKS010000016        31017 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJSKS010000016 DAJSKS010000000
VERSION     DAJSKS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.016"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..331)
                     /locus_tag="ON396_000668"
     CDS             complement(<1..331)
                     /locus_tag="ON396_000668"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112798.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(345..602)
                     /locus_tag="ON396_000669"
     CDS             complement(345..602)
                     /locus_tag="ON396_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112799.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(797..1339)
                     /locus_tag="ON396_000670"
     CDS             complement(797..1339)
                     /locus_tag="ON396_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112800.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(1360..2451)
                     /locus_tag="ON396_000671"
     CDS             complement(1360..2451)
                     /locus_tag="ON396_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR3112801.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(2459..4669)
                     /locus_tag="ON396_000672"
     CDS             complement(2459..4669)
                     /locus_tag="ON396_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112802.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(4685..7213)
                     /locus_tag="ON396_000673"
     CDS             complement(4685..7213)
                     /locus_tag="ON396_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3112803.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(7265..7675)
                     /locus_tag="ON396_000674"
     CDS             complement(7265..7675)
                     /locus_tag="ON396_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112804.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(7688..7906)
                     /locus_tag="ON396_000675"
     CDS             complement(7688..7906)
                     /locus_tag="ON396_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112805.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(7903..8895)
                     /locus_tag="ON396_000676"
     CDS             complement(7903..8895)
                     /locus_tag="ON396_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3112806.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(8974..9885)
                     /locus_tag="ON396_000677"
     CDS             complement(8974..9885)
                     /locus_tag="ON396_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3112807.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(9904..10248)
                     /locus_tag="ON396_000678"
     CDS             complement(9904..10248)
                     /locus_tag="ON396_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112808.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(10268..10824)
                     /locus_tag="ON396_000679"
                     /pseudo
     CDS             complement(10268..10824)
                     /locus_tag="ON396_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10905..11405)
                     /locus_tag="ON396_000680"
     CDS             complement(10905..11405)
                     /locus_tag="ON396_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112809.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(11406..11951)
                     /locus_tag="ON396_000681"
     CDS             complement(11406..11951)
                     /locus_tag="ON396_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112810.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(11948..13204)
                     /locus_tag="ON396_000682"
     CDS             complement(11948..13204)
                     /locus_tag="ON396_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR3112811.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(13565..13876)
                     /locus_tag="ON396_000683"
     CDS             complement(13565..13876)
                     /locus_tag="ON396_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112812.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(13899..15209)
                     /locus_tag="ON396_000684"
     CDS             complement(13899..15209)
                     /locus_tag="ON396_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCR3112813.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(15823..16275)
                     /locus_tag="ON396_000685"
     CDS             complement(15823..16275)
                     /locus_tag="ON396_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112814.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(16277..16642)
                     /locus_tag="ON396_000686"
     CDS             complement(16277..16642)
                     /locus_tag="ON396_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112815.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(16741..21024)
                     /locus_tag="ON396_000687"
     CDS             complement(16741..21024)
                     /locus_tag="ON396_000687"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HCR3112816.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(21171..21410)
                     /locus_tag="ON396_000688"
     CDS             complement(21171..21410)
                     /locus_tag="ON396_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112817.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(21500..21772)
                     /locus_tag="ON396_000689"
     CDS             complement(21500..21772)
                     /locus_tag="ON396_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112818.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(21855..22106)
                     /locus_tag="ON396_000690"
     CDS             complement(21855..22106)
                     /locus_tag="ON396_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112819.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            22269..23228
                     /locus_tag="ON396_000691"
     CDS             22269..23228
                     /locus_tag="ON396_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3112820.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            23398..24078
                     /locus_tag="ON396_000692"
     CDS             23398..24078
                     /locus_tag="ON396_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR3112821.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            24168..25202
                     /locus_tag="ON396_000693"
     CDS             24168..25202
                     /locus_tag="ON396_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCR3112822.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            25216..25551
                     /locus_tag="ON396_000694"
     CDS             25216..25551
                     /locus_tag="ON396_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112823.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            25535..28015
                     /locus_tag="ON396_000695"
     CDS             25535..28015
                     /locus_tag="ON396_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112824.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            28071..29630
                     /locus_tag="ON396_000696"
     CDS             28071..29630
                     /locus_tag="ON396_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3112825.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            29627..30991
                     /locus_tag="ON396_000697"
     CDS             29627..30991
                     /locus_tag="ON396_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3112826.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
BASE COUNT         9440 a         6415 c         5064 g        10098 t
ORIGIN      
        1 atgaattcat tccccaaagg ccttttaaga ttgatacagc agtccgttca cttcttttag
       61 acttgtcata caagtctttt aaatgatccc atatcagact agtttgtcta tctagcttca
      121 atccattaaa cgctaatgaa cggttcattt tttctattac tttagataaa gctacagaaa
      181 tttttatacc ttcacaacgc gcatcttgga tttcaataat agtatcatca attagttttt
      241 gaatttcttg cctatcaaca tattcaatct tagaaagaga atcttttaaa gctactaatg
      301 attcaacgat ttcttttttc tcttcagaca ttttcttcct ccttctagtg acccgtaaag
      361 ccatgtagag cagagtccat tgaaccagta attacactat ctacaacgcc attccaattt
      421 actctacatt ttccattttt cagatttgtc ggtccatacg gatagccttt ttgacattca
      481 atgttcggat tataccagat aggattatac cattcagccg cagaagcaga ttcagtcgat
      541 acaaacaatc ccacaccaac aaatccaata cacatcacac tatagccaat tagttttttc
      601 atctcgtatc catctcctaa aaaatagaat agttcttata gttattcata agacatattt
      661 tttccgtttt tgttttatcc tttctgtcct ataacaactt ttttcaagta aagaatgaca
      721 tagtttttct taaaaaaaca gtctgttttt ttctatttct tcggtgtata ataaatatga
      781 gtgttcattt tttattttaa tacgcactcc gtacataata aatcttgatc gcatcaatct
      841 tttgagcttc ttgatctact ttcaattgaa gtaatacaaa ttgagtaacc ttattcattg
      901 tttcttctcc aaataacaaa tattgatccg gattctctat atcttgtgtc acctggtaaa
      961 gtttcccagt tgttttcatt tgtacatgcg ggtctacatc aatactattg tctttaatac
     1021 aacgatctgt catatagtcc ctcacagctt gattgcgttt ataaacatct gtgaaattac
     1081 tccattttct agcaaaatct tttaagaaat tttttgtcat ttcatctatt tgcatttgtt
     1141 gcgctgtctc gtcttctaat gaaccaattt gactgttact ttcagtactt tgaagagttc
     1201 cacttgtttc ttttactgct tcgattgttt cttctgtcgt tctcaattgt gttttttgtt
     1261 tttcagaaga aaaccaatga caaccagtaa gacctatcaa acataaccaa ctaaatacta
     1321 aaattatccc ttttttcacg tttctagtca ctgcctttct tatttcacct tacgaaccag
     1381 actacgaatt tttgtttgat tgtacgtgcg ttgataccga gccataatcc gtccttgctc
     1441 ggcattctgt tccacactag agaaatgttg tccgccatat gaaacatctg ttataattcc
     1501 ggtgtgacca tagattccag gagacaatgc accacctgct tgccaattca ccacatctcc
     1561 tgcttgtaaa tcacttggtt ttgggttcat gattacttgc caaccatagg ttgtccagtt
     1621 ataatcttcc cctattaaca tcgcaaacat gtgtccagaa cccattaact gtgggccacc
     1681 taattgagat acataccatg ccgtaccacc ataacattcc ccattattta cggatcgtcc
     1741 aatgatccga tccaaaacct tcgcattgcc tttacttttt cctgcaataa gcgaagtttt
     1801 ttttgttggt aggctattcc aatgcttgat cgcaaaagta tcatattccg ttaagttata
     1861 ggacgttatg atactcattg ctcctcgata atacggactc ccttcggtaa tacctgctgc
     1921 cgcatagcct ccacgaccta gaatcaataa acaacttcca ggatctttat tgcccacaca
     1981 ttgattaaag ttgctttggc tcgccaccat aaagccataa tagtcataac aagcttttaa
     2041 gttcggaaac ttcatataga aaccaccttc agaaccacca attccacgtg cagaaccttg
     2101 tgggtaggga caaccaggaa accaggtaat cccaaaaaag ttcgtatcgt ttcttccaga
     2161 ttgtgactgt ccataagctg attcatagcc taattgagaa agaatcacac tcggtaataa
     2221 ctgatacttt acagctgcac caactgctgc ttgcgcatgt tcatctgtga ttggaccacg
     2281 tcctttaatc tctgaaaaaa tcggtaatcc ttcggtatat tccccattaa aagcgacagc
     2341 tagacctgta gcttctcctc catgctcaga aagttctcct gaaaaaaaga atggcaatag
     2401 aaaaaaggga agaataaaga aaagaagaca acctagacac cctttcttca tttcttcctc
     2461 atcctttctt cgataatggt ttttggactg ttcgtccttt tggtaaagta aaatcagttc
     2521 ctggttttgc gtccaaaatc ggcagtgttt taggatcctt ttctattttt tccgattgct
     2581 ctgttggctt atctaaagaa gaaaatgcag gttgtaacga ttttggtgcg gaaatcgacg
     2641 gtttactttc agacgaacgt tgccccggtt gttgcacaga acgatttcct gatagagtgg
     2701 ctaccgacga attaggaaca gagtattggt tcaataactg ctgcattgct ggataactcc
     2761 catttttcgc ctgatttttc acttggcgaa tctgttgtga aagagaacca gcagctgtta
     2821 attgtggcac gggtactttt ccagaaggca acggaatcga ttgaccaaca aaagcgatac
     2881 ctttatttaa caatggtttt ctttgattat tcccttgttg cttactcaat tgtgccaatg
     2941 attgtacctg ttgttttggt cttactgaat tcatggacga atttggaatc aaaccatttg
     3001 aaagaccaga attccactct gttttcgatt tggtagaaga catttcaggc tctaccccat
     3061 gataacgaac atgacgacca ctataaggca aggcttgatt tgaacgccca cctagttcaa
     3121 agggattaac cacttgcctc ccttttaccg attcttgtgg tgtacgtttt tgttgaaact
     3181 tttgaaatcc atgtatcgct gcagaagcga gtggattcgt tgctcgatac acattccctg
     3241 ctaatcctgc acctttgatt gccaaatttt gtccagcagt tgatagttga ccaggcagtt
     3301 gtttcatttg agacaaagaa tgacttgcta tgtttttcgt gttgtttata gtttgatctg
     3361 cagctcctaa ccctagtttc tttaataaat ctttccgttt tacaatgagt aaaattgata
     3421 ggagtgcttt gacaaacata tttcctaaaa aaccaaaaac agtattcatt ggaattaaat
     3481 cgtctactat gttatagatc aaaaatgcca ccaaaataat cacactgtaa aaagattttt
     3541 gaaacatcaa gcccaaaaag gactttaagg tattcatgag catataatca aatactggaa
     3601 caaaagaagc gagtaatgaa aaaggaatca caagtaataa acacaacacc aacatttgaa
     3661 agataaaacg aatcatgcca atcactacaa gtggtaatcc aatacttaga ttaagcaatg
     3721 gcaccataga accaacgact gctttataaa cagctttagt tggttgaata tagacacgat
     3781 aagccttctt tttccctcta tcttctcctg cagcagtggt cacttttgtc tcaatatcag
     3841 ataattcttc acttgacgct tccttggcta gaaattcttg tggttcaatc tctgcttgat
     3901 acaattcttc taaatttgtt gtcccataat ttaacaatat atacggatca acaacagccg
     3961 ctttataata aagttggcgc actttttgaa tggcttcctt ttgtgtcgtt ggcactaaaa
     4021 caccattgac atcttttgtc gccaattcat ctagactatt tgtagcagaa aaaataaagc
     4081 cttctacctc cacggacaaa tcatttaacc ctttggtaaa agcttctcct ttactaggta
     4141 cgttactacc atagccaaac caaaccattg aaaaaatgac aacgaagaga aatcgaataa
     4201 acaccatttt tgcgtaatgt ttccctctaa atatacgaag agtaaataga tataacacat
     4261 acaagacgac aatccccgca ccaataggat cgtatatttt tgaataaata gtatccgaaa
     4321 aagtgaaaaa ctggtgaatt aactcgttca ttgcagctcc ttcatagagc ttctcaatga
     4381 caaaatcaag gccttgccaa atcaatttac ttaaactaaa gaacatattc gtcatcattt
     4441 ttagaaaatc tgtgccattt gccccaaata acattttgcc taatgaaaat tcactatcag
     4501 ataaataaga agaaaaggta tcaatatgaa atttcccaat ctcatagatc gattgatcca
     4561 cctgcttttc tgccgcatac accaatccac caacaaaaag cagtccgaat ccaatcgtta
     4621 aaaaaaacaa aacgcaccat ttcttttcct tatgctttat ataagccatt gtttcacctc
     4681 atttctattg catgattaac gtatcctgtt cttctacttg agagtgactt gttttttccg
     4741 ttgtttgtaa tgcagcggtc atttcttcaa acaaactgtg tacattgagt tttcctacac
     4801 gtccataggg atctaacatc caacactgtc ctttaatcat gtggctcatt tcagctaagt
     4861 tcgcttcatt tacttccaaa cgtaaatgat tcaaaatctt ttctcgctca ctgtcttcat
     4921 caaaagcaaa gacgacacca aaattccctt tttctttatc tgaacctaag tcaccaataa
     4981 attgtgtatc taacactaat tgattcatct gggaacgccc cacacgtgcc attgctgcaa
     5041 taatactacg tcctgcgttc gaattattaa atacccatgc ttctgtaaaa taaatcgctg
     5101 ttttcttact actatctcgt tttccaaaca tttcacaaaa acgacctaaa ctaatcatca
     5161 aacacaaaga ctttcgatcc gcatctgtat atagttctgg tcggacattg tcatttggta
     5221 atttcaaatc ttggatctct aaaatcgttg ttttggcatt aaagtctaat cccgggtttt
     5281 ctccataaga aaatcctaaa cgtaaaatcg aatcttctac cgtcaagcgt aaaaactcac
     5341 catattctcg aacctctttt tgttccattt gttctaaata gtcgaccaca tgcatcaagc
     5401 caacttgttg tccggcttct ttttgaacta aaacttcttg gattcccttt aataccgccc
     5461 cttttaaaag gtgattggta cctaacggac ttaactcatc aatcatatcc tgggctactt
     5521 gtttcgcatc tactcgggat aaacaaacaa ttggatctaa tacaccataa ttacggggat
     5581 ttgtagcatc taacgtgaca taactcattt tttgcaaatg ttctacaaac aaagggtaat
     5641 gtttctgata atacgggtct tgaatgagtt tcgtaaacca tttgcgcttt tctcctttgg
     5701 gatccacaaa taaactttgt acattcataa acgaactata caaaaagagc aagccaacta
     5761 aaaaggtctt ccctttaccg gtttttccag ttactgctac atgaggcgca tcaaacaacg
     5821 cacctttgat tccttccgca atggctagaa gattcaaaaa gacaaaacga tttgacgcat
     5881 aaattaattc ttctaatggt gttttttctc ctcgagaacc accatcccct aatgtatctt
     5941 gccgaccaat ataccagccg gattccatac ctaattctga tactgctgaa aacaaccatt
     6001 cgccaactgc ttctacagtt gttcggtgct tccaatgtaa ctgccgtccc aatggttgcc
     6061 ctaataaaca tttatagaac aattctcgtt gatcattttt tgcacggtgg atatggataa
     6121 tcgtatgaag ttgttgaata accgttgcac tattttgacg aacttcttca accgtttgac
     6181 cgtatacaat aaaacatgcg atccaatcga cgacaggttg cccattatct actcgttgct
     6241 ttaaatgttt tactaatcct cgaacagtta ataagcgttc actaggttct tcatcatttt
     6301 gaaccaattc cttatgctct gttttgaacc gttccgatag ccaattcagt tttaaaccaa
     6361 tacctccctg tcctttcaag cgtggatagt gaattttcat tcgcaattct gctgggaaag
     6421 aaagatcctg agcaaattga aatagatcac aatttctcat attttcaggt gtttccccaa
     6481 taggcaacaa tgctaaataa ctactcccta actcatcttc taattgaaga aggcctttat
     6541 gttccttcgg tatacgtaca tacgttcctt gattgtcttt cagtacttcc caattatttt
     6601 gttcttctac tttgtgagcc attcctcgaa tatattgcag acgattgaga tacaacagct
     6661 ctttttttac taagggacga atagaaaagc tataaagtaa ttgtgtcact tcttttacat
     6721 aaggatcact ttcagaaaac aaagtttcca aatcatctac ttctaatcca aataagcgtg
     6781 ctaaacttcc tgctaaatct aaccagatta gatttattac atcatgaaaa ctctccaccg
     6841 ttgctttttc tttgactttg attcctaaaa taaatgaagg acgatagact tgaccttctc
     6901 gttccaacaa tgtcactgtt tcttttgcat aatacgcacc aattgcttga ctatcgggag
     6961 aaaatccctt tcctaatgtg tcaaacttcg tccgtaaatt cattgttcgt ggatacatca
     7021 ccaactccac atcttcataa tctgctaaaa gcaacagtaa acgagccatc atttctttta
     7081 tttcctgtgt cttttttggt gtgttttttg aaattgttcg tggaaaaaca tgatagtagg
     7141 cccagataca atcctctcca tttaacaaac aatttttctt gaatgctttt actggttctt
     7201 ttacaagcac caatgaattt cacctcccaa taaaaaagga acaaccagtt gattgtccct
     7261 ttttctaatc ctctaaataa acatacttat tcacaaaaag taattcttcc aatgcattgt
     7321 ttgatacctt gtcccagtta taatacacac aatcaccatt catcatacga atcatataac
     7381 aaaaccaatc ccataaataa aaatgcactt tttttccatc aggttttagt tttgccagtg
     7441 ctaacacaca taaatagggg agtcctatat aaaaaacaac tcctgctggt ggaaaaaaag
     7501 aaatgaccca accaataaat gaacgtaaaa ataaatattc aaagattacg accaacaaaa
     7561 aagttgctgc attgactaat gcaactggaa atggaagacg ccaattgctt gtaatctgtt
     7621 gtacgcgata aggttccgca taaacaatcg aataatcata aggttcccaa tccacttcac
     7681 tatcctccta atccataaat aatttttcaa tgattttacc aaacgcatca aatacacgtg
     7741 ttggatcatt taagaagaaa taaactgcgg cacctgctgc gatagatatt aatgcatttc
     7801 ctacttttga tttcataaaa tttcgcaatc ccagataaac aaccaccatt attaaaaaat
     7861 agccaccttg cgttaatata aaatctacaa ttcccttcag tgtcatttcc gttcttctcc
     7921 tttcctatcc aaccaattat gtgttaattt ttcaacataa tactgatttg tttttttcgt
     7981 aatgacaatc gtcatttgtt ctttttgtaa aagtgacgta tcaccatctc gaaagacgac
     8041 cgttgctttt acaataaaag gttttttcgt ttctttataa cgcaatactt tcatctctac
     8101 ttgatcaact ttagctgttt gattgagtcc ttcaggttct ttcatcatgt atgacatatc
     8161 ttttgcattg tttttcgcat acttatctaa gaaatcttcc acaaatcctt caacatcttt
     8221 cgcttccgac gcatcaaccg tttcgtataa tgtgtcatca taagtaatca ttcgttgttt
     8281 ttctgtattc tttggtagtg ccgtaaaata aggataagca ataatacgac agcccttgtt
     8341 tgaaatagaa aagggaagat tgagtacttg tgtgattgtc acacttgttt ctttcttact
     8401 ctctggattc atcgagcgaa tatcataagt tactttataa ctagcagttg gaatggtttc
     8461 aatttctttt aatccaataa acgaagcagc agttaatttt cgaacacctg aaacacttcc
     8521 tggttcttga ataaagtttt gtgcataaaa ctcttttttt aatgtttctt gacgttgttg
     8581 ttcttgttca tgattatcga atgtattcat atacaaagca acaaaacgat tcataaagga
     8641 ttcaatttca ggtgtgtacg ttaattctga ccgactttgt acttctttct tttgtaattg
     8701 ttggatttca tctttcaaaa acgacacttg aaccttcgtt ttcatggatt gaaaaaatgc
     8761 caagccccct gaaaaacaaa gaaataaaac caaacaccaa atccatttca ctgctttttt
     8821 tgaatggatt ttcttttgct tcggtacttt ctctgtataa actttttctt tccgccgtag
     8881 ttgtcgtttt ttcataagct actccttcta gtatgttttt actagtacgt acgattgaca
     8941 acgataagct tgtttttctg aaaaatagtc cattcaatta ccctcatttt ctattccttt
     9001 tctctttctt ttatatccaa caaatccagc aatacttgtc aacactcctc ctagaacaga
     9061 aagtgcaaca tgtatttctt caccagtctt tggtaagact tttgtttttc catctgctgt
     9121 tttgatttca atattgccac ttggaagttt ctttacagga atcgacaaat cctttaccaa
     9181 attcccttca ctatcttgga ttaatggaat accatctttt acaccaacaa ttgtttgatc
     9241 acctgctact tttaatgggg tggttggtgt tggaatatgg gtcaattcct ctacattact
     9301 agtgacaatc ggtacactca ttccttgaga agtattcgtc gtaatttcac cgcttggggt
     9361 taccgatact gtgacttctt ttgctggaac aataggttta ttttcttttg gaattgcttc
     9421 tgatttttca acaggttgtt tcggttcttc tttttgttct tcactggtag acgacatcgt
     9481 tggctgttcc gtacttgacg aatcagtatt cttttcagaa gaactggttg ttcctgattg
     9541 ttccggagtt gtgactatac ttgaatccgt tgttgttgaa ctatgcacac cttcttcttc
     9601 gatcgaatca gtcggtaaag ttgattcagg aaactctggt tctggtgtca ctggctcttt
     9661 ttcaggtttc tctgactgca attgttcagg ttctgctggt ttttccgttg ttcctccatt
     9721 tagctctgtc tcaacatctg gtaatccttc ccctggcaat gtttctacaa tttctgactt
     9781 aggtgtttct acagtttcat ctgcataaac caatgaactc cctaattgaa acgaaaataa
     9841 tgaaattcct agtgttgtta cgactgctaa tgttgttttt ttcatgatta ttcctccaat
     9901 tttttatgca attcgtgttt tgttcataga cgccgcacta atattatctg aacgataaaa
     9961 gaactcccgt ccgttttctt ctaacccaac atatggatta tttttgaata attgaatcaa
    10021 tagttcactg gtgttgtatt ttggatagtt tgcaaaaaaa taatactgtg cttgtcgccc
    10081 atccgaaaat tcaacaagta attcataacg ataccgcttc aatccttttc gttgttttaa
    10141 aggatctcta taccaacttg tccatcgttc cattcgtgga tagagataga cacgatacac
    10201 atagaacaat gccataccac ataccaccaa aaataataat cgccacataa aaattccccc
    10261 ttttcttcta cctttcttga atcatcgaac attcataccc tctatagaga tttgaaaaat
    10321 ccatcatcaa ttgttcatat aaacgttgtt gcaaatagcg attgtttcga atacataatt
    10381 cttcatagcc atcaatcact tgatcttctt tttcatagca tcttgtagct ctatgacgct
    10441 cagcttgcat gacataacca aactctgcac catatcgttc tactgttaat aactgttgac
    10501 tacgcaataa tgcaatataa caatcacaaa aatgttcaac cgacatttga tttttctcac
    10561 gtttgtgaaa taggtattct ttagaaagca cattattcgt taaacaatct gataaatagc
    10621 gataatctgg cggtcggtta ttttcatctg tgaatttttc ccataataaa ctgattttta
    10681 ttcgttcagc ggctagatag tcttgttcgc gtggataaaa ttcatcaatc gcacaaagtt
    10741 ttttatcacc tgaataaaca aatccatata atgcgaaacg aactcttcct tttcaattgg
    10801 ctctcctaaa aattccagtg acataccatc ttctccttcc taaaaaaaat aaaaaagcag
    10861 aaaccgattt ggcttctact tttctttcct tttatagctc ttttttacgg aagaatccgc
    10921 aaatgacatt ttttcaaaaa atcaatcggc acaagttcac gaaaacttgg agcaattttg
    10981 gatagaatca cttccccatc ttgtaaaata caatcataaa aatcatcagt tacttgatac
    11041 ttacgcttct tacaataatt gataaaatag tagcgatcat tttctatttc cattcgttgt
    11101 tctactaagc gcatacgaac atcatcatag aaataagcca caaatcgccc ttcaagttca
    11161 tgtttttcaa tgggatactc ttctggtaac aatctatttt ttcgtcgttt atacattgct
    11221 ggatacctta gaagaagttc atctaaacgt ctgtcagtat atggtaactg acgaatcaac
    11281 gattctaatc gttctttata cagatagaat aattggcgca agcaatagtg taccgcctct
    11341 ggattttcta atactgttgg atcacgtgac caatacgcac caacataaat cataattttt
    11401 ttcatttaga caatcacacc agcaatacta atgaccgatc cacaataggt agaacaagtg
    11461 gcatagcgac caatttcctc taattctatc tcaatctctt ctaaagaaag atatgtctgt
    11521 tcttctgttg gctggcgact catttcccaa gtgacagtca ccaatgaaat ttcttccata
    11581 agaggttttt cgctgctaag tgctgccaca cagttttcat atatcttcat tttatcgcag
    11641 ccttttttaa ccgctaaaat cgcaggtatt ggcaatgaaa atcgataaaa gcgaatgaat
    11701 tctagccttc ctttccttcg gctaaattcc aactcttcga gttcttgttc gtatccctta
    11761 cgaacttcta ataagcgtgc aatttcttgc cagttctctt tttcctgata acgtccagct
    11821 tttaccgctt gttcaatata aggaactcga aaacgaaaat aagacaaatc aaccatcttt
    11881 tcatttaatg cttcgattgc agcctttaaa tccaacaggc gcagccattc tgttgtcgta
    11941 tatgcattca tgcaattttc cctcctgacg ttcccatcca ttctagatag ttttgttgct
    12001 cgtcatttag ttcggcatta tcaatccctt cttgtacaaa gtctcttcct aaaatacgat
    12061 ctgcttcttg taacaacttt aatgtcggag ctacttgttt caaaaaccag cgttctgaac
    12121 gatctaatga gaatttttct ggctttactg taaaatccaa ttcttccatc gaacgaaata
    12181 attttgccca tcgtttatca acaatccgtg catcactttc cagtctatcc caaacaataa
    12241 gttttgcatt aattaaacca acgccaatct ctcctaaacg tttcggtgaa tcacctaaaa
    12301 taaatgcttc tactgcttgt gttgctttct cattggctaa ccgtaactca tatcgattat
    12361 aaataccata tttcgttaat gcttctaatt ctgaaatttt ttctcggttc gcaatttcaa
    12421 aacgtttctc ataaaaatta aaaaagagag gactctttcg cgatccaaaa tacaaagata
    12481 atccttcact tcgaaaatag ccatcatcta aaaaacaacc gccaatattc tggaatctct
    12541 taaagtccat ttctaataaa cctgcttttt gtttttctag taattcaaag agatcgaaat
    12601 gttcttccgt ttccaatccc caaagttcat ctaacgcaat atcgaatcgg ctacattgca
    12661 cacgtgtata accagcttcc aactttcctt gatttttcca aatggaatat agccatgttc
    12721 gccaatctgt ttgttgttca cgcatgactt cctcatattc ccggcaacct tgtcctgaaa
    12781 attgcacaag tacattttct gttacagttc gttttgaatg ccagaacacc cagatattcc
    12841 cacgaacaga aagacagtca aaaagataca agcccgttgg acgtgtttca aattctgtca
    12901 gatccattcg taatacacgt tgaatcactg ttttgtagtc tgtcccaaaa aaggaaagac
    12961 gcaaataatc aatcattgct cgaagatcta ttccgctttt catatacaat cgaaagcgtt
    13021 ccttgattcg ttgttcaaaa tcctctggga tttttctttt cccttgttcc atctttctaa
    13081 tgtaatcttt tgagcagccc atcaattgcg caaatcttgc ttgtgatagc ggctttttat
    13141 ctccacttat attatgaatt gcacttaact tctttctgta gctttttagc tcaatcggtg
    13201 tcattttctc gctcctttta taaaataaca aaatcgattt tttttaaccc tttagaaaca
    13261 ccgatatatc aactagtccc cctcgttttt tgggaaaagt agtacaactg ctgaccttag
    13321 ataatcactt gatacaaaag ggtttataaa acttttttct atgtgttatg ttatttctgc
    13381 cccccatatt atatatcggg gggctttaca caaagaggcc gtgcgcaagc gcacgacctc
    13441 tttgtgagct gcacacgctg ctagcgagtg cgcattatag gcccatgctt cgcagcctta
    13501 atgcgcaccg ccaggcagag tgcggctacc agactacgtc tagagccctc ccgatagata
    13561 ataactacaa aattaaatga tctaattttg ttatttcact atatttttca ggagaaaaaa
    13621 cgcgataaat tgacaaagtt aaatcctctt ctaaatctac tcgataatga tacaatcgag
    13681 tcaatcgttc tatatctttt ttcgttagca atacttgatt aataactttt cctgtaaaaa
    13741 cacgttcttt gcgcaaaaag cggtaatcag ttacttgttc aatacgattc aaccaatcat
    13801 aaaacgttct ggaagaaaga gaaggattaa tcattcgtat tgctgtagct actctataat
    13861 aacaactctc tgccatttaa cttcacctcc ttagttgatt acaaaatatg cgcatcagaa
    13921 aaacttgttc cttttggatc ttttgctaaa tacatttggc gctctagttg ttcttgtaac
    13981 aactgattat catctcccat cgtttgatct tgactatcct cttttactgt aaaatcatag
    14041 acaatcattt cagtaaattt ctcaaattct ttgataaagt gataatcttt gtaaacaaat
    14101 ggcgcaaaaa actctacagg tacatcaaat cctggaaaag catatccacg accaggttga
    14161 tctttgttgt aaaatggctt atttttaaaa tcttctccaa aagtcattaa ataaccaatg
    14221 ggagacaatc gaccaacaga aatacggaat gttaaattat cccgaacacc acttggaaaa
    14281 tattctgcat ctggtcgttg cattgctaaa ataatgtaca tccctgcttg acgacccttt
    14341 aatactaatg gctgaatcaa tttatcgact ctatttctcg cttccatatc caacgaacca
    14401 taaaaagctg cccattcatc aaaaaagata aaatgcggtg gtaacccata ataagcataa
    14461 tttttccctg gttcatattt aggatctttc atggttttca tataggcata ccgttgattc
    14521 atttctttta atgcatttcg taaacaaata atcatttctt catttatttt gtaaaacaca
    14581 tgtcctttga atacatcgac gtcacctaat gctgctaaat ctgccgcttt tccatcgcaa
    14641 atatctactg tcccaacact taaacaagct tgaattaaag aatacaataa ataggttttc
    14701 ccaccaccga ttgcaccagc aatcaacata tttggtgatt gatcatatac ccaatccaaa
    14761 gttttcatta aatgaatacg tccattttct gctatacatt gatcaatagg aatccgacca
    14821 gaatctgaaa ggaatacata attgattcgg gctttttctc gttcttcacg aattaaatca
    14881 cgcaaaaata cttcagataa caaatgaccc atttttaaaa atcgttcgtg aaaacgccca
    14941 ccatctaaag gaaaagaaac cactaattct tgtttctttt gataaaaata aacttttggt
    15001 aatctcattt tttcccttgt ttgtttctca ctaacttgtt tctttgtata aacaaaccga
    15061 ttgtcaatta acattcgaac aagtaactga cgccaataaa cagaggcaaa atatccttct
    15121 tttagtcgcg accaatgcat caaccctaaa attaacccac taaccagcaa caaatacaac
    15181 acaatactta ttccaggaat aagatacatt tgccaattcg aaaacgtact taaacgtgga
    15241 taaaatactt tccaactaaa ccaacctaac agaatcaaaa atggaaacaa aaagaaacca
    15301 taatagcaaa accataaata acgattgaaa tagcgaattc gccgaccacg atacttgaaa
    15361 aaatgagctt tcaattgtca cattcctcct ttctgttttt acttttcctg tgctaaaaac
    15421 ttttgacaaa aagcaactaa tacaggaata tcacatttag taaaaaaaca taatcgaaaa
    15481 agatttaatc gatttttata agggtgtgca cacatattac atattcgtac tatacgaatc
    15541 caaatcactt cctgatgatc ttgtttccac tggtacggta atgaaaaacg aacaagatca
    15601 cgtaaaaaca tacactgccc aataaccgca caaatatatg catttgtttg tacaaaaatc
    15661 gttggattgt ctccaaaata ctcaatcaat tgttggatat tttgataaca agcacaatct
    15721 ctcaaagtat ttgattggtt ttatctgaat tcaaaatctc cccttccttc tatcttagat
    15781 aaaaagaata ggaagactcc ttcctattct ttcaaaaaaa ccttattttt tctctttttc
    15841 cttagaatca acacttggtt gtttttgatc taaacctttt tcttgtgatt gtttcatatt
    15901 aggattattt gttggagctt ctactttctc taatcctttc gcataaatca ctaaatctga
    15961 atattcacga ttgccatctt gtgctggaat atataccaca tgatattctg gatcaattaa
    16021 tttcacagga tcacgtcgtt taaaaccagt caaatctaca tctgctggaa atttaactcg
    16081 tcgtgcaatc tttggttcat ctggatttcc aaaaactaat tgtgctcgct gtgatcgaat
    16141 ttctcccgta taatcgcctg tttcattatc aaatacacga tcagactctt ctaatgcgcc
    16201 aaagcgtacg gctcctaaac aagtttcatc taatgcttct gtaatgtcat aattatttgg
    16261 aaattttaat cccatcttac aaaacctccc caatttttac accagttaaa aagacaccaa
    16321 catcttgata gccagttgtt cgacgctctg tttgtcccat atcatttgtt tcacgactat
    16381 tcactcgttc tttacgaaaa acaaaataag cttcttcaaa tgaaatacgc acctcttttt
    16441 ctggtaatcg taatctttta tcctcttcag ctgtgagata aacaaaaaaa gcaggctgtt
    16501 cttctcgcga acactctgct tgtaattgaa catatcgccc agacacacca ttaatttcct
    16561 ttggtggatt aggttcccgt gaacccaata aaaatacttg acctaaaaac tctttcttca
    16621 tacgcaatgc tttttctgcc ataaagtttt tcctcctctt aaaaaccaaa ataaattcta
    16681 aatatttcta cattacaatc aaacaagcgg ttaaaaatcc taaccgcttg ttcctcatga
    16741 ctaatctttg gctttacgta ctttataaag tgttatacct aaagcaatga ctaatactac
    16801 taatccaaga accataagta tactacttgt ttgttcacct gtttttggca acattggtac
    16861 aacagtagct ggtttaaccc gttgttctgg tttcactggt tgcggtggtt ttggcgcttc
    16921 cacaatttga accgtttgat ctttgtttgt tagatctaca ttgtgtttcg ccacttcttc
    16981 aaacgtttct ttctcatctt ccgttttcgc ttggagccat tccgcaaaac tcaagccttt
    17041 tcctgcaaga ttgcgaccgt ccactaagaa ttcaaataag aattccatca tttctttatc
    17101 ggccgcaaag actttttccg catgagtgcc ttcaaatggt gtgccgtctg gtgttaagta
    17161 gccttgaaca attaagcgat agatttgacc aatcgcaaga ccgtctactt tgacccattc
    17221 aagcacagtt tgatttggca aggccaagat ctcttttccg ccatttttat tcgtcgcttt
    17281 cgtcgtaatg gttggattac tgattttgat ggtttggtca ttgtcggtaa tatctgcgtg
    17341 agaagccaat aatacgcctt ctctaaacag atcttcaaag accacgactt ctttgcctcg
    17401 caacgcacgt gcgtctagaa taaagtctag attgactgta ccatccgttg tttctgccac
    17461 aaacgttaag gtacttgtga cttcttttcc atctactagc actggttttt ccgttacttt
    17521 gtccattaga acaccgttga ctgtgtatgt ttttccaacg accaaatcgt ggtatttcac
    17581 agcgtctgta atggtgactt tcgttaatgg attaacctct ttcaagccag tgggtttatg
    17641 tgtagccgtt gtacgtacac ttgggttagt tactcgtacc gtttgccctt tgtcgttgat
    17701 gtctttatgg tttgctagtt cttcatcatt tctaaaaaga ctttcaaaca cgacgatttc
    17761 acgaccattt atacctgtca aatcaaaggt aaatggtacg atcaccgttt cattttctgc
    17821 tttggcaata aagccaacac tcgtttctac cgtatttcca tgcacaagca agggttcacc
    17881 ggttgcttta tccattaaga tccctttgac caagtatgct tttcctggaa gaatctcggt
    17941 aatttcaacg gtatcgttta aggtaaacaa tcctgttggg tctgcgactg tcaatccgtc
    18001 ttcaaagaca gccgtggtat gaacggttgg attattcact ttcacacttt gggcttcatc
    18061 gttgatatcc gcatgtgctg caacttcaat gccatcacgg aaaagttttt caaaaacaac
    18121 gatcgtttga cctttcagaa cactagcatt aaccacaaat tcaaggtctt ttgtgccatt
    18181 gactttgtct gcaataaagg ccaaggtact tgtgatttct ttgccatctt gtaaaattgg
    18241 cgcattcgtt gccttgttca tcaatactcc tgaaatggtg tattcttttc cgacaaacaa
    18301 gttggtataa gaaacactgt ctttaatgac gatcgtttct aatggatcaa ccactttttc
    18361 accatcggct ttatttgacg cttgtgtttc tagttttggt ggaatcaagt aattacgtac
    18421 agaaaccact aacgtttccc catcatctgg cgcattttgt ttgaatgcaa ccgtgaatgc
    18481 ttgtttatct gtatttggtt ggaaactttc taatggggca ttttccaaga agtaatagtc
    18541 atcggcaact agacctttca ctaaaatttc cccgttttga tcagtggtat attcaccaat
    18601 tgcttgattt ttaccgttat acaaggtaaa gttcacacct gcgagcggcg ttttcttctt
    18661 gttccattct tcatacttga ataattttac acttttccgt gcttgacggt tcgcaaaatg
    18721 gatcactttg atcggttctg tatcattggt aatcgagaac actaatgggt tttgattcaa
    18781 aacgtaattt acgggggctt tgatttcttc caaacgatag cgatcttttc cataagtaag
    18841 tgtaccgtta gttaggaaaa agcctttttc attggtttta aagatatccg ttgtttctgt
    18901 atcatttgga atctccattg tgacataacg attggctaaa gtatcaaaaa tcttgaattc
    18961 tgtattagca agtggaatca cttttcctgt ttcttgatcc actttcacca ctttaagacg
    19021 ttgttcaatt actttatctt ctaaaacata atggaaagtt tgcccatttt cagaaattgt
    19081 gacttcaagt ggttcaaata gtttataccc ttcttttcct ttggtttcag aaagaatgta
    19141 cgtatcaaag acaacggtat ttttaccaaa agtagcataa ccatcattgt ctgtcacaac
    19201 ggttttcacc actttgccag ttgtttttga cgtcaaagtg atttcaatgc cagctaattt
    19261 tggtttgtta tctggattcg ttgaatcgac taaatcttta ttagcaactt tcactaaatc
    19321 aatggttcct ttgatcactt gatcttttgc ggtcgcattg acagtttttc caggttcaac
    19381 aacaatttca attgcttcat cagaaagcac ataacctttt ggcgctttga tttcttttaa
    19441 aacatatttg ccaggatcaa gatctttaat ttctgcctgt actttgccgt taattttttt
    19501 caaggtaatt tctttaatct ttttcccatc tgcttgatac aaaccataga ccgcattttc
    19561 taaagaagct gcaccttgtg ctttcacacc agtttccgca tcttctttaa acacacgaat
    19621 gtttccttgt tttagaattt gtaaattaat aacagacgca ttaggttctg ttggatgtaa
    19681 cacaccaatt gtttgagctc ctggacgacg ataggctaat ggcgcacctt gaactttaat
    19741 tttattcaat tgaatttttg ctttatccgg tgcctctttt gtgactgtaa tccatacttc
    19801 attgccgtga atagatacat tgacaccttt tggtttttga atgtctttca catatttcaa
    19861 aaaatcattt ttattcttta ctttcaacgt ttcgccaatt tttaccgtat gtgtttgatt
    19921 attaaaagtt gctttttcac caaagttatt aatcaaacgt tgaatttctg cttttcgacg
    19981 atcataatca acacggatat ttttgattgg gatttttgtg cccaaagctt cccaaatcat
    20041 aaattgtgcc gcaatgtatt gttcatcgga tttgtcttta tttgtgacat atcctaaata
    20101 agagatcaag cttgtttttt tccgtacatt tgaatttttc aaatagtttt tcaaagcagt
    20161 agctgtataa acttgacctt ctgcggctga gacaccaggc tcaatacaaa acgccacttg
    20221 tccattaatc tcaaaacgaa ccatataatt taaagtgaag tttagattgt tcggactaga
    20281 agtaacacca atattttcgc cccaatcaat tttccgacca cctaaaacag ctgcattggt
    20341 tgcacggcga acacgaaccg aacgattggt tggtactgtt actaacagtg taggtgtacc
    20401 tgtttttgga atgttctgct cttttttctt ttgattttca atactagccg tagattcttt
    20461 gtttggtttc gttggttgat tttgactagc tgcggttgaa gagattgttt tttcaacgtt
    20521 ttcctcattt tttccttctt tgtcagcagt agccgctggt tgttccactg tttcttctat
    20581 tcgtttcttt tcttccgctt gtttctcaga agactctggc tgcacagaag gcgttgcttc
    20641 ggcagctttt tctccttcta gagtggacgt ttctgaagca ggggttgtaa ttgaggcagt
    20701 ttcttcaact ggttgtgaag taccaggact attccctggg tgttgtgttt ccccaatcgt
    20761 ctgatcgaga gtcgttttgg aattgaccgt tggcgcttct gtcacagggg cctgttcaac
    20821 agtggcttca ccagctggga ccccggcttc tgctggttgg acttctgtca cttcagaagc
    20881 gacttgggca atggcttcca tcgtaggttg tatagccgtc gtttcttctg catgtgcttg
    20941 agcagttccc accaagaaac agaattgaga aaaagccacc aatgcgagtg ctttcaacca
    21001 attcattttc gtttttgcat acacaagaat tcctcctttt cctgagaatt ttatataata
    21061 aaacaaaacg ccgttaccaa aagtgataac gacgtgaact gtctcttcta tttttatctg
    21121 gcgctcttat ctgaacacat ttcatcaatg attttaccct cccactataa ttacagaaca
    21181 aaaaaagaac ctgcttcgtg gaaatcaact gtccatttcc caattggaat accagctttt
    21241 ttggcaactt caccatcacc aaatctcatg ccataccaat tactaccttc atccttaatt
    21301 tctttccaac catatgacat agcttcatct aatgaatcaa aaatcatgac actattgcca
    21361 attgttacag gatctggctt aagatcttct ggaagagaaa tatcaatcat accataatga
    21421 tccgattcct cagtattatt gtcattacta gctactggtt tggttgttct actggcttat
    21481 tttgatacaa caagtactct tattcttctt ttacttctcc gttttcaatt tcccattcaa
    21541 accactccgc aaagtcttgc ttaaaaccat ctactcgata cctattttgt aaataggcat
    21601 cttctgacgt cgtaggatct aaataggtag gtttattctc tggacgagca tagacaccta
    21661 cccatgtttc gtaatctcct tcaaaataac tatctaccaa atagcctttt tttctaagaa
    21721 tctcccgaac ttgattttta attattgata cttgttcaaa accatcactc attctatcac
    21781 ctcaaataac tctagattct cataaatatt cccgacaatt tcaatcctat caatgatctc
    21841 taacaaatcg ctacttatgc ttccccattc gtataagaat ttcccttccg caaaataaat
    21901 tttccataaa cttcttcatg atcgtcccaa ccaatatcac tctcgaaaaa ttccacgttg
    21961 ttcttatctt ttagctcagt aaatgccata agttcaacat gttttccaac ctcgaaacaa
    22021 tcacaccacc catcaaccgt tacacgtgtg ccttcaatac ctaagaaact ttgtcctcgt
    22081 gtattaaaca ctaaaacttt ccgcattttt tgctcattaa ttaatcacgc gcgaaatcta
    22141 ggaatcacaa cttattcgct tcttctttat aaatcgccga ttgtaaaatt aagctagaca
    22201 actaaaaaaa gtcatttgtg ctacactcaa aatagttccg acgaaagaat tataaggagt
    22261 gagtacaaat gacctataac catcttacac caactgaact cgtaatgata gaagcttatt
    22321 tcaatcaatc acaacctgtt tcaaaagtag ccaatctgtt acaacgttct cgacaaacta
    22381 tttataaggt ctatcgtttt cttaaatctg gtggaagcgc aatgagctac tataaacaat
    22441 ataagaaaaa caaacgtaac tgcggaagac atccgattgt tttacctgat gagcaacagg
    22501 aatacattca aaaaaaggtt gttcaagggt gggctcctga tgtccttatt ggtcgagcag
    22561 agtttcctat tgcttgttcg gtacgcacac tgtatcgaat gtttaaagat aaaacctttg
    22621 acactcacga tttaccaatg aagggaaaaa gaaaaccaaa cggtcataaa gaaaaaagag
    22681 gaaaacaagg gtttcgccgg tcaatccgag acagaaagac agactatacc cagtttgatc
    22741 atgaatttgg tcacctggaa ggtgacacta tcgtgggtgg aaaacataaa agcgcggtca
    22801 tcacattagt tgaacggcta tctaaagtca tcattacctt gaaacccgaa ggcagacagg
    22861 ctaaagacat tgaaaatagg ctgaatcaat ggtttcgttg tgtcccaaaa aacttattta
    22921 aatccatcac atttgattgt ggaaaagaat tctcaaattg gaaaaagatt agtaatacca
    22981 acgatatttc aatttatttt gcggatccag gaacaccttc tcaacgagga ttgaatgaac
    23041 attcaaatgg cttattacgt aaagatggtt tgtataaagc tatggatttt aatggtgtat
    23101 ctgaaacctt tatccaatcg gtggcatcga aacgaaatca tattcctaga aaatcattac
    23161 actatcggac accaatggaa gtatttttga gttacgtcac aaaagatgat ttgtctaact
    23221 taatttgaca aacgaaactt aataaaaaag gaagaatatt catgtaaacg attttattgt
    23281 ttttttctct tttatacaaa aagggtcata atttcaagta agcggatgaa ttcatgagga
    23341 ttttttgcat actgtctcta ctatacttag gtcactaaga aaaaggagtg atttattatg
    23401 aaaaagaaca tgattgggac attattactt tcaacacttc ttctaggcgg cttggcaaca
    23461 ccagcttttg ctgagggtca agcgacttca aaaggagata ttacctttac tgaaccaaca
    23521 aataccgtgg agcctcttaa ccctactgat ccttcaaaac cagtagagcc agcagatccg
    23581 gaaaatccag caactggcca gacaggtcct ttaacactgg atgtagttcc cgaattgcca
    23641 tttggtacac acgagattga aagcggaaca aaaacgtatc aagtagacgc ttcaaaaaac
    23701 gatacacctt atctccaagt atcagatcgt cgaggtgtag gtgcagatgg ccaagcacaa
    23761 gggtggaacg tgacagtttc ggtttcagat tttgtaaacg gttcacaagt tttgcaaggc
    23821 gctgaattag attttggaac atccactgtt aaaagtacgt cagataacga atccactgga
    23881 ccaactagtc aaactgtaac tgggttgagt aaagcaagtg cagcgacacc aatatttaca
    23941 gcagcaaaag atcaaggtct gggaacatgg ctttctgttt atgatccagc aaatatcaca
    24001 ctgaaagtgc cgaaagcagc agctggaaca ttcacggctg atttgacttg gaacctcgtg
    24061 gctggcccag tagcatagag tatgaaataa attcaacaaa ctgcggtgaa aatactatag
    24121 gtgaaggatt ttttaccgca gttgttgttg aaaggatgtg gtggacaatg aatcaaagta
    24181 aacgactgtt tgtttcgttt ttttctattt taagtctatt tttcattttt ccgtcaatca
    24241 gccaagcaaa ggattcagca ggagactttg gaatcaaacc ggtatttcca gagaaccaaa
    24301 tagataaagc aatcggttat tttgatttat tagtggcacc ggaacaaaat cagacactag
    24361 aagtaattat cagcaattct tcagatgaag aacgaacatt tgaggtctct gtcaatccgg
    24421 cggtaacgag tgatggtgga acgatcgatt atagtcaaaa aaacccaacg ttagatgaga
    24481 cattgccgtt tgatgtccgt gacgttttgc tcattgcgaa aaaggaaatc aatgtatcag
    24541 cacatgcaga aacaactgta cctatagaag tgaaaatccc agcaaaaagc tttaagggac
    24601 gtgtattagc gggtattcat gtttcaccta aagaagaagc tgaaacagaa aatgctacag
    24661 aaggagcaca aatcaaaaat cgtattgctt ataatctcgc agtcgtttta caagaaagtc
    24721 aagagacgat agaaccggat ctgaaacttt tatcaggaga tttggacgag gtcaacgcaa
    24781 agccaactgt tcagcttcgt ttccaaaatc ctcagcctag gattatcagc aatttgatat
    24841 ttacgagcaa gatcttttat gaaaatcagc tttatattga aaatacgtct aatgcatttt
    24901 tagttgcacc aaattcgaat tttcacttga atcttgattt agctggtgat aaagcaaaag
    24961 ccggcgatta ccgcgcagaa atcatagcaa aatcaggcga cagtaatgaa tggcgattta
    25021 cacaaaactt tacgatcaaa aaagaaaaag cacaaaaagt aaatgaaaat tccgtttttg
    25081 cagtacaaga acaatctttc ccatggcttt atgcaggatt ggttgctgca gttctagtgt
    25141 tatggttgat tttgctgttt ttgtgggtcc acaagaaaaa gaaaaaagga gaaacaacat
    25201 gatgaggtga tttcgttgaa aacatcgatc ttattgggag ggattattgc aagtttgctg
    25261 ttcttcagct ggcaagagcc agtccaagca gataccaaaa gttcaggtac gattacattt
    25321 tatgatctaa gtccaattcc gcatccaagt ggttcagtca atggcgcatc aaaacagcag
    25381 acacagctac cgatttataa agaatctaat caacatggcg tattcccgga gacgaatgaa
    25441 caaaagatcc gttttcttta tttgatcatt ggtatagaat tatggcttct tgcgtggttg
    25501 ttgttatgga gaagaaagga aaaggaggat gaacatgttg ctacaaaata aattttcatt
    25561 ttttcgctcc ttctttttta cttcgctttt tgttttaagt atatttatct tttcaccttc
    25621 ggcagaagct gttggagcaa cgcctcctgc catttcttat gatccataca cgtatgaaaa
    25681 tatcgtacct ccaaatggtg gacgtagtgc agtcaatggc gggaagcttg tccaactaac
    25741 tggggctaac caattgccta aattgactgc tacttatttg aacgatggtt caaatgatcc
    25801 ttggaaaggt ggcacggtcc gttttcattg ggaaagccat gtgaaactaa caaatgtact
    25861 tgatttggtg aatatcactg cacaaggatt tgcatttgtg gcgatcatca atttgccgga
    25921 acatgtatca agcagtgatg tattaaaagc aattgactgg aacaacgcct acttgtatat
    25981 tgccggtgcc cccatcaagg ttactagcgg aagtttatta tcagcaggaa gccatacctt
    26041 gcgttttggg atggggtcgt gggatacaaa tactctattg acagatatta ttgctattat
    26101 tacgcgaggc gaattcaacg tggacgatat cccgcttcaa tttgatgtta cagctaatgt
    26161 cgcgaagatg acagaaaacg gagataaata tgatacgagt ccaaacaaaa ttttgaccaa
    26221 gggtaaatta caaccttcta tgtctaaaaa ggccgttttt tctgttgatt tttatgattc
    26281 gaataatatt atccaagatc gtatgttcaa ttcgatttta gctccagctg caggtagatt
    26341 ttatcctaaa ttaactggcg gaaacttgaa tccaggagca tatgcatttt ctgcaagtgc
    26401 agagataaat acgtggaata gttacatctc accaagtgat tcaacaggaa aatataatac
    26461 atttgctgat accacagaat acgtggatgg aaataaccag aaccttccag gctctaccaa
    26521 tagtcggaca ttgacgatgg ggctgactga aggacgtttt gatcagttgc catttagtcg
    26581 ctttgatcgc gtagtcaact attttacagg aaaaaacgtt acacaaggag caatactgac
    26641 tcatactcca gcaagcacaa gtggattgaa tcagacagcc aatatcgtgt acagtgggaa
    26701 agatgcgcaa ggcggattgc tttctcctgt tgctttaaag gtattggagc agtacaagct
    26761 agacggaacg gttttaccaa caaacttaac agccgatagt cagtggggaa agctcctttc
    26821 ttctgcatcg taccaagtca cggccaattg gtcagcaact gaactaaaaa agggttcgat
    26881 ccattacagg ctttattcga aaggaaccca aaaattagcg acaggatgga cagatcaact
    26941 tttccaaacg atccaatcaa ataacggggg tgtaaataca gctacttcta cactacctgc
    27001 attggcttca gggcaatatt attttgatta tcgcttggtg gacgatgtac ttactaacat
    27061 gtatccgtct tttgcgtaca aatggcagtc tgaacaagct ggaatcacca ctcttcctca
    27121 aataacagtg gctaattttc catctgtgag tgcagatagt cagctaaaaa accttagcag
    27181 acaacctgag acaggagatc ccctgtctgc tctatcagga gatacgattc aagaaaatgt
    27241 gacattcacg ttaacaaaat ctggggatgc gataaccgac aaaaaaatca cgatctcact
    27301 tcctgaaaat actacttata tcaatggttc cttaaaatta aatggtacaa ctatcgcaga
    27361 taccggcatt caacaaggag tttctgtccc agcagatcta ttgagcaaaa taggagatac
    27421 cattcatctt acttacaact atcaattaaa tactgtagat acttctttac aatccgtcag
    27481 catactgaca aaggcagctg tactttccag caatattaca ttagcagatg gtgctaagct
    27541 tcctaaccca gttgtccaaa catctgctaa aacaattctt gtccccaaac aagaattaac
    27601 attagttaat gtaccagatg attttacatt tggtaacgat ctccccaaac cgttgaagac
    27661 aagttattat gaatcaaaag gcgatttttc ttttgatgtg cgggatactc ggcttccaag
    27721 cacgtctcct tggcaactga caggtacatt gacttcttta tttaagaatg atcaaggtca
    27781 agaattaagc ggaacaaaat tatacttcaa tcattctgga tcaaagcaat tgattcagca
    27841 aggtcaaaac acgttgattt atgagagtga tggaacagct aaaggagagg ttctcgttga
    27901 ctttcccgat accgatggtt tactcttaga ggtaaatagc agtacgaatg ctcaacctgg
    27961 cgcaacgtat caaggcatgg tcacatggga actgacagct gggccaacca gctaagtgat
    28021 tagaatttgt atggatctgt gataactgtc acagatcctc tttttcaagg atgagaagga
    28081 aacttctgct gttaaaatca gaaatgcttg aaggagtgga cttggaactt ttcagtttgc
    28141 ttgaaaaaaa tgaacaacgg caaatcaaac ttttccaact tttattagag gaaaaaggag
    28201 aggtttccgt taataacgta cttacccgct tatcagttga tcgggctacc ttgaaagaag
    28261 atctgtttta tttacaagct aacttggaaa atatcaaaca attggaaatt gttatccaag
    28321 aagggaatat tttattaatc agacatggaa atatagcagc agtcgatgtt tattatcatt
    28381 gttacttgcg ctattctaca aaatatcaga tacttcttta tctgttggaa aatggctcat
    28441 ttgatcggca gaaacttgtc atggaactta atatcagttc ggctactttg tctcgcagag
    28501 tgaaggaatt aaatgattct ttaaatgagt ttcatctgaa aataaaaagc gggcggctga
    28561 ccggtccaga aagtcaaatt agttattttt atttccaatt attttggttt ggacgtccct
    28621 atcttgaaaa caaaaaagaa tacagtgatt tatcaggaaa cactttttta gatctgctgc
    28681 aaaaagagtt tagttttgct attacagaag aaggacgatt gaaatttgaa atatggcttt
    28741 ccattatgaa gaagcgaatg cgctgtacga aaaaatccaa accagattat agcaatcttc
    28801 ctgttggatt gtttcaaacg acacaatttt tattggacct ccatacattt ttgacccgtt
    28861 acttctttca atatgccttt ccttggaatc aaaatgaaac gttggttttt tatcttttca
    28921 tgatcagtaa ctttacatta gatacacata actcgcatgt ccaggagttt ttgataagcg
    28981 aaaaatacaa agacgacgta gtagctcaat tgaatcgaac gtttaaacaa tcgatagaag
    29041 aatatttctc tttttatgtt tttccagaag agatttcgca aaagattgcg ttgacagtga
    29101 tgcaaaatca cgctcatttt gcctatttta gaggatggat gaatattttt ggccaagaag
    29161 gattaaaaca gcggttaact gaagtagaag ataaacaatt cctggtttta tgtgaaagat
    29221 tggtcagaca aacatttgct attgttgatt ttactgaagt agacgaggaa aatgcccgct
    29281 ttgaattatt tgctcgctat gcatctattt tacggacgct gatcaagcag ttagaattac
    29341 gtctgcaaat tgcttatgat cttccttatg aaaggctaat ggcggatatc gtgaaagaaa
    29401 tcatccagga acaattgctg aatacgatca aatatgattt agtcgaatac gagaaagaaa
    29461 agcagtatga tatcattttg actagccaat tgaaagagta cccgtcacag aagtccgcta
    29521 aagtttttgt attcactagc aacgaatata attatgattt cccctatttg aaccaatttt
    29581 taaaagaatg ttatttagaa aaattgaatt taaggatgaa taaaacatga aaatcgtgat
    29641 cattggtgct tcccatgaag gcatcacagc tgccctaaat ctaagaaaac ttcaaccgaa
    29701 agcagaagtt ttactgatcg atgaagatca taaagatgga ctaggctatg tttcaaatgg
    29761 aatcaacctt tatttaaaag gaaagatacg tagtttagct gaagttgccc ataacatgcg
    29821 gactcttcaa gaaagtgggg caaaactgat caccgaatgg agagtaactg aacttgatcc
    29881 agacaaacat cagctcatcc tcgcatccaa agaaggaaaa aacgagacca taacctatga
    29941 taaattaatc gtagcgacag gttcctctcc tgtcaccttg tataaacaaa ttgaagctga
    30001 aaatgtctac acctataaaa atctagtgca atctaagcaa gtacttgccg ctttaaaaga
    30061 agcaaaagaa gtagtgatct tcggtgctgg ttacattgga ttggaattag cagatgcttt
    30121 gagaaataaa ggatacatta ttcatctagt tgattatatg ccgaatgttt tatcaagata
    30181 tttcgataag gatatgatca acagttttca aaatcagctg caaactaagc aaataaattt
    30241 ttatccaaat gaattcttga tcgattggaa aaaatcagaa gagaaagtag tatccgtaca
    30301 acttctgtct caaacaataa aggcagatat ggtcattttt tctgcccaaa ctcgtcctaa
    30361 tacgacattg ttaaaagaaa aagtggcttt gtatgaggat gagactgtga tagtcaatga
    30421 atatctgcag acaagtgatc cggacatcta tgcgattgga gatatcgtcc cagtcagttt
    30481 tgacaaaaat aaacggcatc tctttttacc tttagttact cgagctgtac atatggcaag
    30541 agcagtagcg ctaactttga gtggtcagcc aaccgcttac gacctcagac aaaagataac
    30601 agcagcagtc attatagact actttttagg aactgtaggg ctgacagaag atgaagcgcc
    30661 attcttggaa caaaataccg gttcgtgttc aggagaattt gatctatttc cacaatacga
    30721 cgaagaaaac aaaacagtaa atgcaaagct gatctaccat ccggatacat tagaaataat
    30781 tggcggacag ctgataagcc aagaattctt attatcagat ttgaacctgc ttgcggatat
    30841 cgtgaaacac aaaactacga ttccacagct agcagttgag gattttggat ttctgtctga
    30901 atacacctct cgttttcact acctcaatga gttagctttt aaagtactag ctaagagagc
    30961 aaatagcaat agagtagata aaagactgtg agaaatatca cggtctttta ttgtgtg
//
LOCUS       DAJSKS010000017        29494 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJSKS010000017 DAJSKS010000000
VERSION     DAJSKS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.017"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..750
                     /locus_tag="ON396_000698"
     CDS             <1..750
                     /locus_tag="ON396_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR3112827.1"
                     /translation="KKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVLKIL
                     DTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETIHLG
                     VLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLEKHP
                     LIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAFSVS
                     MPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            850..1494
                     /locus_tag="ON396_000699"
     CDS             850..1494
                     /locus_tag="ON396_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR3112828.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            1501..2508
                     /locus_tag="ON396_000700"
     CDS             1501..2508
                     /locus_tag="ON396_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR3112829.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            2524..3366
                     /gene="kduI"
                     /locus_tag="ON396_000701"
     CDS             2524..3366
                     /gene="kduI"
                     /locus_tag="ON396_000701"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR3112830.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            3386..4189
                     /locus_tag="ON396_000702"
     CDS             3386..4189
                     /locus_tag="ON396_000702"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCR3112831.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(4438..5502)
                     /locus_tag="ON396_000703"
     CDS             complement(4438..5502)
                     /locus_tag="ON396_000703"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR3112832.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(5499..6584)
                     /locus_tag="ON396_000704"
     CDS             complement(5499..6584)
                     /locus_tag="ON396_000704"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCR3112833.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(6597..7574)
                     /gene="mvaD"
                     /locus_tag="ON396_000705"
     CDS             complement(6597..7574)
                     /gene="mvaD"
                     /locus_tag="ON396_000705"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCR3112834.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(7567..8511)
                     /gene="mvk"
                     /locus_tag="ON396_000706"
     CDS             complement(7567..8511)
                     /gene="mvk"
                     /locus_tag="ON396_000706"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCR3112835.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            8827..9486
                     /locus_tag="ON396_000707"
     CDS             8827..9486
                     /locus_tag="ON396_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR3112836.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            9571..10476
                     /locus_tag="ON396_000708"
     CDS             9571..10476
                     /locus_tag="ON396_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR3112837.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(10478..11110)
                     /locus_tag="ON396_000709"
     CDS             complement(10478..11110)
                     /locus_tag="ON396_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR3112838.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    11261..11386
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            11430..11954
                     /gene="infC"
                     /locus_tag="ON396_000710"
     CDS             11430..11954
                     /gene="infC"
                     /locus_tag="ON396_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR3112839.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            12026..12226
                     /gene="rpmI"
                     /locus_tag="ON396_000711"
     CDS             12026..12226
                     /gene="rpmI"
                     /locus_tag="ON396_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR3112840.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            12279..12638
                     /gene="rplT"
                     /locus_tag="ON396_000712"
     CDS             12279..12638
                     /gene="rplT"
                     /locus_tag="ON396_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR3112841.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            12890..14227
                     /locus_tag="ON396_000713"
     CDS             12890..14227
                     /locus_tag="ON396_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HCR3112842.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            14275..16404
                     /locus_tag="ON396_000714"
     CDS             14275..16404
                     /locus_tag="ON396_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HCR3112843.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            16379..17068
                     /locus_tag="ON396_000715"
     CDS             16379..17068
                     /locus_tag="ON396_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112844.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(17476..18089)
                     /locus_tag="ON396_000716"
                     /pseudo
     CDS             complement(17476..18089)
                     /locus_tag="ON396_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(18225..18419)
                     /locus_tag="ON396_000717"
     CDS             complement(18225..18419)
                     /locus_tag="ON396_000717"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112845.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(18469..18909)
                     /locus_tag="ON396_000718"
     CDS             complement(18469..18909)
                     /locus_tag="ON396_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCR3112846.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(18913..19758)
                     /locus_tag="ON396_000719"
     CDS             complement(18913..19758)
                     /locus_tag="ON396_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCR3112847.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            19907..21325
                     /gene="pepV"
                     /locus_tag="ON396_000720"
     CDS             19907..21325
                     /gene="pepV"
                     /locus_tag="ON396_000720"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HCR3112848.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(21382..22329)
                     /locus_tag="ON396_000721"
     CDS             complement(21382..22329)
                     /locus_tag="ON396_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3112849.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(22552..22902)
                     /locus_tag="ON396_000722"
     CDS             complement(22552..22902)
                     /locus_tag="ON396_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCR3112850.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            23102..24181
                     /gene="pepA"
                     /locus_tag="ON396_000723"
     CDS             23102..24181
                     /gene="pepA"
                     /locus_tag="ON396_000723"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HCR3112851.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            24325..24645
                     /locus_tag="ON396_000724"
     CDS             24325..24645
                     /locus_tag="ON396_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR3112852.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            24667..25131
                     /locus_tag="ON396_000725"
     CDS             24667..25131
                     /locus_tag="ON396_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR3112853.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            25336..25941
                     /locus_tag="ON396_000726"
     CDS             25336..25941
                     /locus_tag="ON396_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCR3112854.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(25980..27269)
                     /locus_tag="ON396_000727"
     CDS             complement(25980..27269)
                     /locus_tag="ON396_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCR3112855.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            27697..28176
                     /gene="rlmH"
                     /locus_tag="ON396_000728"
     CDS             27697..28176
                     /gene="rlmH"
                     /locus_tag="ON396_000728"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR3112856.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            28261..28770
                     /locus_tag="ON396_000729"
     CDS             28261..28770
                     /locus_tag="ON396_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR3112857.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            28870..>29494
                     /locus_tag="ON396_000730"
     CDS             28870..>29494
                     /locus_tag="ON396_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112858.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPI"
BASE COUNT         9320 a         5485 c         6147 g         8542 t
ORIGIN      
        1 aaaaagcctt atggaactgt attgatcaaa gcctctaaaa tactagacta tttagcggaa
       61 aaccaagatg tctctttgca gacgatcgca aagggtgtgg gaatgacctc ttctacagtc
      121 ttgaaaatac tagatacttt gttgatgatc ggctacgtaa ataaaaattc agagaaaaac
      181 tatcgtttag gtgcaaagct tattcgctac gcgaataaaa acattgaaca aatcgatttg
      241 gtagagttga ctttaccgtt tttagaacag ctgcagcaaa agatcgatga gactatccac
      301 ttaggggtat tggacaacaa tgagatcttg tacgtgaaca aactagatcc aaaacatcaa
      361 acgatccgca tgtcttcaaa aatcggaatc acccgaccat tatacaattc tgcgatgggc
      421 aaagccgtat tagcagaatt cagtgaagag cagtacactg attatcttga aaagcatccg
      481 ttgatccctt acacagaata tacgatcacc aatgcattac ggttgaaaaa ggaaatccaa
      541 acggtaaaag aaacccacat cgcatttgac gatgaagaaa tcgaacgtga tattttttgt
      601 ataggagcct caatcgtgaa aaacgaacaa atcattggtg catttagtgt aagtatgccg
      661 aaatatcgtt taacagatga gacaaaagaa caaataatca gtgccctcaa gcaaacaaaa
      721 agcgaaatcg agcaaagaat tcaaaaataa tgaaaaaagc gtttgcaatt tttcttttct
      781 gccgtataat atgggtatag atttccaagt tataaaataa aatttctaaa taggaaatag
      841 gtggagaaga tgaaaaaaat tgatatttta tctcgattga aaaacgccgg agtgattgcg
      901 gtggtacgcg gaaaaagcaa agaagaagct ttgaatgctt gccacgcaat catcaaaggt
      961 ggtctaactg gtattgagtt gacttttaca gtccctcaag cagatcaagt aatcaaagaa
     1021 cttttgtcat tctacaaaga ccaaccagaa atagtgattg gcgcaggcac agtattagac
     1081 gctgtcacag ctcgcttggc tatcttagct ggcgcagagt atattgttag cccttccttt
     1141 gatcaagaaa cggcagagat gtgcaatctg tatcaaattc cttacttacc aggatgtatg
     1201 actattaccg agatcaaaac agcgctgaaa agcggggtag atatcgtcaa actatttcct
     1261 ggaagcgctt atggaccaag tatcatttct gccttcaagg cgccgatgcc tcaagtcaat
     1321 atcatgccaa ctggcggtgt gagtctggac aatatgaaag agtggttcga tgctggtgtc
     1381 gtaacagtcg gagtaggtgg gaacctttta gcacccgcag ctacaggaga ttttgacaaa
     1441 gtaacggaag tcgcgcaaca gtatgcagca aaaatgaaag aaatcaagag gtagctgctt
     1501 atgggaaaag tagtgacact cggagaaatc atgttgcgat tgtccacaga gcagggaacc
     1561 cgtatcgatt caagtgaacg attcgctgcc cattatggcg gaggggaagc aaacgtagca
     1621 atttctttag ctaattacgg acatgaagcg atttttgcta caaaagtacc tgataacgca
     1681 ttgggaaaag cagtcaaaaa gcacttgcaa tgttatggag tagatacgaa acaggtatta
     1741 tctggtggat caagactagg tacttattat atggaaactg gtagtggcga acgcgccgcc
     1801 tcagtgatct atgatcgtgc cggatcaagt tttgctgaaa tggatcattt ggaatggtcg
     1861 ctagaggagt tattccaagg aatagatata tttcatgttt caggaatcac cccagctttg
     1921 tctaaaaaat ggcaaaaaat gacgaaacag gtgatagaag ctgcaaaaaa agctggctgc
     1981 ctgatcagct tcgatatcaa ctatcgtgga aaattatgga cacagcaaga agccagcgaa
     2041 gtaatccatc agcttttacc tttagtagat atctgctcag ctggaaaaat ggatgctctt
     2101 tatttgttag gtatcgagaa agcgccagaa gcagaagaac aacctctaat ttattattat
     2161 caaaaaatgc aggaaaagtt cccgaatatt caggtgtttt attcgaccaa gcgtaccgtt
     2221 cattcagcaa gtgaaaatga attgaagggg acattgtgga tgaaccatga atatgtggaa
     2281 tcccaagtcc atagaatcac cccaatcgtg gatcgagtag gtggcggaga tgcttttgca
     2341 ggtggcgtgc tccatgggat tttatccaat atgtctccgc aagaaatcat tgattttgct
     2401 acggcagctt ctgccttgaa gcatacgatc catggggact gcaaccaatt tagtcaagaa
     2461 gaagtcgcac aatttttagc atgcggctct ggaaaaatca ttcgatagaa ggagtagaag
     2521 aaaatgcaag aaatggaaac acgttataca cacagtccag aagatatccg tcattattca
     2581 acggaacaat tgagaaaaga atttttagta gaaaaggtat ttgaaccagg gaaaatcagt
     2641 ttgacttata cgcacaacga tcgaatgatc tttggtggag tgacacctac tgaaaaagaa
     2701 ttagaaatca cattagacaa agaactaggt gttgattact tcttagagcg tcgtgaacta
     2761 ggtgtgatca atatcggtgg accaggattc atcgagatcg atgggcaaaa agaagaaatg
     2821 aaaaaacaag acggatacta tatcggaaaa gaaacacgac atgttgtatt ttcttctcaa
     2881 gatgctgaaa acccagctaa attctatatt agttctgttc cagctcatca taaatatcca
     2941 aatgtaaaaa tcagtatcga ccaagtaaaa ccaatggaga ctggtgaagg cctgacactg
     3001 aatgaacgta aaatctacca atacatccat ccaaatgtat gcgaaagctg tcaattacaa
     3061 atgggttata cgattttaga accaggaagc gcttggaata cgatgccttg tcatacacat
     3121 gaacgtcgta tggaagctta tgtctacttt gattatgcca atgaagatac acgtgtgttc
     3181 cacatgatgg gcaaaccaga tgaaacaaaa catttagttg tagacaatga acaagccatt
     3241 atttcgccaa gctggtcgat ccattcaggt gtaggtacaa gcaattactc attcatctgg
     3301 gcaatgtgcg gagaaaatat cacctataca gacatggata tggtgcaaat ggatcaatta
     3361 aaataagcat aagggagaga aaatgatgga atttagtatg gatatgtttc gtttagatgg
     3421 aaaagtagca ttagtcaccg gtgctgtcta tggtattgga tttgagattg cacgctcttt
     3481 agctacagca ggagcaaaaa tcgtatttaa taacttgaat caagaatcag ttgatgaagg
     3541 tatagcgcat tataaagaag caggcatcga tgcaaaaggt tatgtctgcg atgtgaccga
     3601 tgaagaagcg gttcaagcga tggttcaaca gatcaaagaa gatgtaggtt cagttgatat
     3661 tctagtcaac aatgcaggga tcatcaaacg gacaccgatg atcgaaatgg atgcggcaga
     3721 cttccgccaa gtaatcgacg tggatttaaa tgccccattc atcgtttcaa aagcagttat
     3781 tccggatatg atcgaaaaag gcggcggtaa aatcatcaat atctgctcca tgatgagtga
     3841 attaggacgc gaaacggtca gtgcttatgc tgcggcaaaa ggcggattaa aaatgctgac
     3901 gaaaaacatc gcttctgaat acggtcaata caatatccaa tgtaatggaa ttggaccagg
     3961 ttatattgct acaccgcaaa ctgctccatt acgcgaaaca caagaaaacg gtgaacgtca
     4021 tccattcgat caatttatcg ttggacgtac gccagcagca cgctggggcg agccaatcga
     4081 tttggctgga ccatctgtct tcttggcttc tagcgcatct gatttcgtta acggccatgt
     4141 cttgtatgtg gacggaggca tacttgccta tatcggaaaa caaccttaaa agagctttag
     4201 tacactgttt agtggagagg ttgtgaaatc agcggattga acgaataccg aaggacagct
     4261 gattaaacat catttattcg aaaaaagagg ctgggacaga agcatttaac tccaagaaat
     4321 aagaaggaat tcacgaaaat tgcttttcaa atttttgtga atttcagctt atttccgaag
     4381 gagttgcttt tacttccgcc gtttgtacgt gtttagagcg tgagacaaaa gtgttcttta
     4441 cttttgtccc acgctctttt ctgtgcgtct tccatacttc actaagtcga tccctcttgc
     4501 ttcgcaccaa tccttcactg gtccggaaaa gatcagagat tgttggtgta gggccgcggt
     4561 attcgttgcg ccgaccatag tataaagcag acgaagctct tcttgccatt gcttcatcag
     4621 tataatagtt tcttctacgc cgtgattcat cagatgcgtc agtacggttc ctgaagtacc
     4681 cacggcattt gcgccgagac aaagggcttt aaatatgtca taggcattgc ggatacctcc
     4741 ggaggctagg atttccatag aagtgtctgc ttcatttgct tcaagcaatg aagagactgt
     4801 cgattgcccc cagtcagcta aataactcag ttcccgcttg gagcgacggg cgttttcgat
     4861 ctgggtgaag cttgttccgc ttcgaccgct gatatcaact gtatgaactc ctaaagcagc
     4921 tagatcattg agcgtttctc ttgtcatgcc aaaaccgact tcttttacga tgagggggac
     4981 gtcaatggct gtttgagttt cttgaataag tactttccaa ttggtaaaat cacggtctcc
     5041 ctcaggcata accaactctt gaggtgcatt caaatgaatc tgtaaggcat ctgcgtgaaa
     5101 caatcggata gcttctttgg ctctttcgac agaagtccct gcaccgatgt tggcaatgat
     5161 tttcccgtga ggatattctt gtcgcataat cgtataagta tcggctaaag aagggacttt
     5221 caaggcagcg ctgactgaac cagtggcaat catcaaatct gcttctcttg cgacgattgc
     5281 aagatcttgg ttgatttttt ttgttttttc gcttccgcca gtcatcgcat tgatataaaa
     5341 tgggctgctt agtgtaaacc caactgcctg tgtacttaga tcgacatcag aaactgctat
     5401 ttgcggcaaa gggttatgga tgatccgtac gaagtcaaat tcgttttttt gtttatcatg
     5461 aaaggcttta gctagtgaaa catgttcgtc ttttcgattc atcggttttc ctttctttga
     5521 tcgctataga catgtaacgg cagtggagtg atttctgctt tttcccatgc actcattaaa
     5581 ggaagaatgc cagatttctg gtcaacaatt acgattccgc aatcgccccc acctgcacca
     5641 gaagattttg cggctccttc atactgttca gctaaattac acaatttgtt caaagcaggc
     5701 gtttcgatga cgaccccagt gattgcagat aaatcatgca gtaattgtcg gttttttcga
     5761 atcatctgtt ggatcaacgt tacattattt tctttaaacc ctttgatcat ttcgttgaca
     5821 cattctgtac tattttttaa gaaaagctga taagccacca ttttatcttc tctcgaacgg
     5881 tgaacttgat cgaccaaatc agaagtagag gcagggctac ccgtccaacc aatcaataaa
     5941 cgtaaatctt caggagcaat caatggctca atggatagac ctggccaatc cagggcaagt
     6001 aactcactga tagaatgctg agtttcttgt tcttggagcc aaggatggtc gaaggttgag
     6061 aaagcgatcc agccaccata acagctagca gcgatgtcgc cgcaggaacc attatcttga
     6121 actgctaaat tggctagtgc tgcaatcttg aaaatctcca actgagataa attcaacgcg
     6181 taaaaaacat tcaaggcttt gaccgttgca acggtcacgg caccgctaga acccaatcca
     6241 tatttccggc catttgaact gtctaactca cttgttactt tcagatcata aaaggataaa
     6301 agaatgtttt tttcttgtgc atacttttca gtcaagcgaa tagcagcaag gatataatga
     6361 aaaggatttt cccgaatatc taaaactaat tctccgttgc gtcttgtcca acgtacaggc
     6421 atcccactat attgtgcaga ttggatactt ccgacttttc gtgcagattc tacagtgact
     6481 gtcacgaatt gatcgactgc agcgataact gctggatggc ctgtttcaac aactgcatat
     6541 tctccggcaa tatagagttt gcctggtgca gatacttcaa tcataaaaat ctccctttat
     6601 tcaataatcg caattcctgg accagcatgt gcagtaatta cttgttcttt gctaaataat
     6661 ttggtaaatg tttcttgtac ctcagataaa tggctgtttt ccactaaaac tttgacatta
     6721 gggccagcat ccatcgtaaa gtagcatgga atgccttgct ttctcaattg tcgcaccgca
     6781 tccatcgctt ttaagctgtc aggagaccag taagtgaatg gaggctgggc agctaaagtc
     6841 gttccatgca tttttagtcc gtttctttcc atcgtttctc cgagaagttg gaaatctttt
     6901 gttttgattg cttgtttcaa ttgatataga tccccttcaa cggaatctaa ccagccttga
     6961 taaaaactgg atgtttcgac tgtccgacgc atcccatttc tgctggacac ttcttttttc
     7021 tggtcgttga tcaaaacgaa aaccattgct aaatcgtctt cgaaagaatc ggaagggact
     7081 ggcttagcgt aagaacttaa gtcgtcatgg cctttttccc attcgacgaa accaccgaaa
     7141 atacttcggc aagctgaacc agacccgcgt cgagcaaatc ttgaaagaga gagatcatct
     7201 aatccaagct taagagcagt attgcaagcg cctgctaaag cagctagccc gctggcagag
     7261 gaggctaatc cagctgcggt aggaacgaaa ttctgactga ttactgaagc ttttagtgag
     7321 aggccggctt ctttacgaaa aaggtcaaga aactgactga ctttttttgt tgctttttcg
     7381 tcttgcaagg tgccatctag ataaaattca tctaccatat agctatcaga aaagatgact
     7441 tctgtctctg tataaaatgc atccaatgtt aacgaaaggc tgttgttcat tggaaggatg
     7501 agttcttcat ttttcttacc ccaatatttg attagagcaa tgttggtata tgcgcgtgct
     7561 ttgcctttaa acataggtat gtactcctaa tccttgtatc caagtttcag cagcacccgc
     7621 atcttcaagt gcttgactga tttcttgggc ttttgttttt gtttgtgcta atgcaatcat
     7681 acagccaccg cgtccaccgc cagttaattt ggcacccaat gccccggtat cttttgctgt
     7741 ttggatcaat agattaagaa aatcattgct gattgtcagc ttttccagaa gtgattgtga
     7801 ttcatccatt gtctgtgcca acgtttctgg tgaattttca atgattgctt gttttgcttg
     7861 cttcgtcaag tatcctaatt tttggatcat ttgtccagtt tcatgaggct gggtttcaaa
     7921 gaggtgagcg acatctttga cggcttctct tgtttgtcct ttgattcctg tatcagcgac
     7981 aatcaaaaaa gcatcgatgt tcaaagaaaa gtagtcgaaa ggatgccctt ttgtaaaata
     8041 aatcggctgc aagctgctag tagcggctgc atcgattcca ctaggattac cgtgggcgat
     8101 tttttccgaa agctggacat tttctaatag tatttcacga gacaaaggaa atgctaagta
     8161 atcataaaaa gcacgagtga ctgctgtggc gactgcagcg cttgatccca ttccacgttc
     8221 agcaggaatc gtgctttcaa tggtcagttg caagtttttt tggatgtcat gctgtttttt
     8281 taagtgaatg aataattttt tgatattttt taatgcatga ggagcttcgg ctaaatttcc
     8341 tgaatagtag gaagaaacca gttgatcgtc cattgcatcc agctcttcaa ggaatgcggt
     8401 gacttttgtt gcatagaaag gaaaggcaat cgctggttct ccataaacaa ccgcgtgctc
     8461 gcccatcaat atgatctttc cgcttgattc tccttggcca tagtttgcca taatctaact
     8521 ccttctcacg taaaagtgat ttttaagcaa aaaataaagc gcccaaaacg agttgcgctg
     8581 cttcacgaat ttttttgtcc actaagggtc tcaaaaaaca ttcctccttt attttactaa
     8641 aaccatctca gaagtaaaac taagaatacg ttttctaaag aaaaaacact tttttcttaa
     8701 gaattttttg actttttttc ttctttgact agaaaaaaat acaattgaca ctactagata
     8761 ttgggggtat atttaaaaag gttggagtta aacacaatat ttagtgatga agtgagctgg
     8821 aggtaaatga gcgtgatcgt tttagcaggt acaattggtg ccggaaaatc aagtttaaca
     8881 gaaatgatgg ctgaacactt tgatagtcag gcattttatg aatcgattga tgataatgaa
     8941 gtcttgccct tattttatgc aaaccctgaa cagtatgctt ttttgctgca aatttatttt
     9001 ttgaataaac gttttgcaag catcaaacaa gcaatgaaag atgacaataa tgtattggac
     9061 cgttcgattt atgaagattc actgcttttc catctaaatg cagatctagg aagagcaaca
     9121 gaaacagaag ttcgtgtgta tgatgaactg ctggaaaaca tgatggaaga acttccctat
     9181 gctgcccata aaaaacatcc ggatttatta gttcatattc gtgtatcttt tgatacgatg
     9241 ttggagcgta tcgaaaaacg tggtcgttcg tatgaacaat tgtcctttga tccaagtcta
     9301 tacgattatt acaaagaatt gaacagacgt tacgatcaat ggtatgaaga atacaaagaa
     9361 agtccaaaaa tccaaatcga tggagaccgc tataattttg tggaagatcc tcaagcaaaa
     9421 gaagaagtct tgaaaatgat cgaagaaaaa ttagcagaga ttcgccaaac caaagtagcc
     9481 atttgattta cagttgctca aataaagggt aaacctttat ttgagcgact tttttcttta
     9541 tactggaaac aaatgtcagg aggatagagt atgcgggaaa aacactattt taatcaagac
     9601 cgtaattttt ggatcaatgt acagcaaaac gatgtgctag ctgttaaaaa cttaaaagaa
     9661 gaatatggca ttagtgacga gatgctgact tattctctag ataaaaatga acgggcgcgc
     9721 gtagagtacg attcttttga tgaagcgtta ctgcttgtat caaatgttcc gcaccagcaa
     9781 aaagtcgata atcattatga aactagtcct atcgctttta ttttaaaaga agacggtttg
     9841 tttacattta ccacgccgaa taccgagtac gtgattcgac taattcgatc gcttttggaa
     9901 cgaatgccag acatgagtgt atacagttta ttgtttcgta cattgttttt gatttctgat
     9961 tccttttttc ctttaatcga ggaagtgaat agtgaacgtc agcggcttaa tctgaaatta
    10021 agagaaaaaa caacgaacaa gaatctgctg caactttcag atttagagat cggtttggtt
    10081 tacttagtta caggaacaaa acaaaatgta gtacttttag aacaaatcaa ggcattggct
    10141 atttatcgca agttgtcaga aaaggaaaaa gaagagttgg acgatgcgct gatcgaagca
    10201 cgacaagcag ttgaaatgac gaacttagct tcgcagattt tagaccagct ctctggcact
    10261 tataataatt tgctgaataa caacttgaat gacacgatga aatttttgac ggtctggtcg
    10321 ctgatcttga ccgttccaac tattgtgaca ggattcttcg gaatgaatct ccagttaccc
    10381 tttacccatt ccgttttcgg atggggaatc gccttgatca tcagtttggt cttatctatt
    10441 tggatgctta ttgcactgtg gcggcggatt cgttgatcta gtctttgatc tgcaaaccaa
    10501 gggcttgggc gatcacaagt gcttgctctt ggttgacgat tagtcccgcc aaagaatctt
    10561 gagataaagc gattgctgtg aagaaattag tcgtaaagtc taatcccttc agagaagttc
    10621 gaaaccagtt cgaaccggtc aactgattgt cagacaaaaa aaggtttttc caagtgacct
    10681 cataaaattc gctgtcttct aattgacaat gatcgaagga gacagctttc aagtttgtac
    10741 tactaaagga agccatcgaa gcaatacaat cagtgaaagt acagtctcga agataacttt
    10801 cggcaaaatt cgtacctgtt aatttacatt gatgaaacac gacttggtgg aaactgccac
    10861 caatccactc cagattggaa aaatcacatt tttcaaaaat gacattactg cattcaaaat
    10921 gacgtaaaac tgttttttgc atcgtaagtt tttggatatg actctctctt aataccatat
    10981 ttttggcatc gagatagctt tggtcacccc cgttgatcag aagaccagag aagatctcct
    11041 catcttctaa ataaaactct ccctttgtaa gaagaggaag agaaggtgga ataggttcga
    11101 tttttttcat gtgggttcac ctcgtttttc tttagtatag caaagaagcg ggaatcactg
    11161 ctagaaagtc tgctaatcta atcattttca gaaaaggaag acaaactgta tgaaaaacat
    11221 tgacaaaaag attagttttt gctatactat gatggttgag aaataaaagc agaagcaccc
    11281 gcttctcgcc ttagttgata atgtcactgg gctagatagt tttcactggt tctattaggt
    11341 gaagtacgcg cgggttctgt aagagaactc gtttttttat tgccgtttat tcggtaagct
    11401 tttctttctc tcataatatt tggaggtgaa tgaccatagc aaaggatatg atggttaacg
    11461 acggcattcg tgcacgagag ttacgattga tcggttcaga tggtgagcaa ttaggagtta
    11521 agacaaaagc agaagcattg aatattgcag aacaagcaaa cttggatctt gtgttagttg
    11581 cccctggtgc gaaaccacca gttgcgcgaa tcatggacta cggtaaatac cgtttcgaac
    11641 aacaaaagaa agaccgcgaa gcgcgtaaaa aacaaaaagt gatcaatgta aaagaagttc
    11701 gcttaagtcc tacgatcgac ttgaatgact tcaatacaaa acttcgtaat gcacgtaagt
    11761 tcttggaaaa gggcgataaa gtgaaagctt ctatccgttt caaaggccgt gccattaccc
    11821 ataaagaaat tggtcaaaaa gtcttgaatc gcttagctga agaaactgcg gatattgcaa
    11881 cagtggaaca aaaagcgaaa atggacggac gcagcatgtt cttgacgctg gcaccgaaaa
    11941 acgacagtaa gtaatttgaa tgactaactg gctgttggac agccgatagt cggataacga
    12001 aagattttga ggaggaaata tagtcatgcc aaaacaaaaa acacaccgcg gatcagcaaa
    12061 acgtttcaaa cgtactggta aaggcggatt gaaacgcttc cgcgcgttta caagtcaccg
    12121 tttccacggc aaaacaaaaa aacaacgccg tcaattgcgt aaagcaagca tggtttctag
    12181 tggcgacttc aaacgtattc gccaacaact agcaaaaatg aaataagaag tacaaataac
    12241 agaatctgaa gatttatttt tattaggagg aattatacat ggcacgtgtt aaaggtggaa
    12301 cagtaactcg taaacgtcgt aaaagagtgc taaaattagc aaaaggttac tacggttcaa
    12361 aacacacttt atttaaaaca gcaaaagaac aagtaatgaa ttcatacaac tacgcatatc
    12421 gcgatcgtcg tcaaaagaaa cgcgacttcc gtaaattgtg gattgctcgt atcaacgcag
    12481 cagctcgtat gaatggcttg agctactcaa aattaatgca cggtttgaaa gttgctgaaa
    12541 tcgacatcaa ccgtaaaatg ttagctgact tggctgttaa cgatgcagca gcatttactg
    12601 cattggctga ccaagcaaaa gaagctttgg ctaaataagc taaacaatta taaagaggaa
    12661 agaagataac gaaaagttat gttctttcct ctttttttat ttacaatcct ccatcaaatg
    12721 aaacaggaca ataacgtata agtctaaaat agcataatta aggtttttta attgaaaact
    12781 gaacatttat ggtcaccctt taatgaaagc gctaactaca taagttagaa tgagtttgac
    12841 tgatatatta gtttgcttat cagaaaaaca aaatggacgg aggaataaca tgagtgatat
    12901 tattattggt attttgttag ttatctcatt ctttttcatg gtctggtact gtgtaaaagg
    12961 ctacaacttg atggtcggct ttgccatcat ggctactgta tggatgggat tagcgctagt
    13021 cggaaacacg ttttcaccaa acccggcgat ggaagggcaa ggagtaattg atgtgttgac
    13081 gcatatctat acgacggggc cagctgagta cgccaaatcg atcttggtaa atgtcttctt
    13141 tggtgccttt ttcgggcgtg ttctagtaga tagcggaatc gcagctaccc tgattcgaaa
    13201 ggtagttgaa cttggagggg acaagccaag aatcacgatg agcttactat gtattgtaac
    13261 cgctgttata ttcatgtcga tgacaggaat cggaccagtg atttctattg cagttattgt
    13321 gttgccaatc ttgatgtcat taggtatttc agtaccggtt gcactgttct catttatggg
    13381 ctcgattatg gctggaatct ttgctaatat cgtcaatttc aaacaatatc aaacgattta
    13441 tgctggtttc aatccagcgg ctgaaagcta tacttataat gactatttcc aaattggtat
    13501 gattgggatg attgtaagct tagttgtggt actaacggtc gctaatatat caatgaacaa
    13561 aaagaaacgc tatgcaatgg cagcaaacgt accagctgaa ggtggcgacg caccaatgat
    13621 ctcatggtta gccgtattat taccagtttt aggggtcgtt cttttagatc ttccaatcat
    13681 cttaggtttt attctagcag gtatctgggc attgctgttt actggaaaat tgcgcggagg
    13741 ttataaagaa atctgtcgtc agtttgcgaa attatttaca gatggcgcag tagatgttgc
    13801 accaatggtt ggtttcttga tgaccttagc gatgttcaat aattcagctg cgtatgcttc
    13861 tccttatttt tcagctattt ttggagattg gataccgcaa tcaccattag tcttggcaat
    13921 cgtatttgct atattgacac cactaggatt tttccgcggt ccaatgaatc tagtcggttc
    13981 aggctcagct attttagctg tcgtattagc tgttaatcca acgatgtcgc cagccttttt
    14041 gttcccgtta tttgcaatca caacgattgc tcctcagcat ttagatatta cacaatcttg
    14101 ggttgcttgg ggattaggtt atacaaaagt aacttctcga gaatatatga agaaatccat
    14161 cccaactggt tggatcatcg gagcgatctt atgcctgatc acgttcttat tgtacgggaa
    14221 cgcctagtaa acaaaacgtc gataaaaacg attagatgaa tggaggaaaa gaaaatgaaa
    14281 cgttttgtgc gaatgggaat tgatgttggc ggtacacata cgaaagcagt cgcaatcgat
    14341 aatgcgacgc acgaaatcat cggaaaatct tcagtgaaaa caacccacga tgatgtccgt
    14401 ggagtagccg caggtgtcgt tcagtctttt caaaattgtt tgcgggaaaa taatatcagt
    14461 ccagaagatg ttgtgtttgt tgcacattct accacacaag cgactaatgc actgattgaa
    14521 ggagacgtag caaaagtcgg cgtaatcggg atggcaaaag gtggattaga aggtttttta
    14581 gcaaaacgac aaactagatt gaacgatatc gatttaggga ataagaaaaa aatagagatt
    14641 gtcaatgcat tcttacctgt caaacactta aatgtagacc gtgtttcaga aacgatttct
    14701 tctttagaaa gagaaagagc ggaggtcttg gtttcttcta tggcatttgg agtagataat
    14761 ggggaaccag aacgagttgt ttatgaagca gcatcggtaa aatcgatccc tacaacgatg
    14821 gcttctgaca tcacaaaatt atatggattg acaagaagaa ctcggacagc agcaatcaat
    14881 gcatctattt taccaaaaat gctagatacg gcaacctcta cagaagattc cgtacgagaa
    14941 gcaggggtca atgtttcttt gatgattatg cgcggagacg gcggagtaat ggaaatcaat
    15001 gaaatgaaaa aacgacctgt tttaaccatg ctttccggtc cagcagcatc agtcatgggc
    15061 tctttgatgt acttgagagc gtctaatggt gtttattttg aagtaggcgg aacaacaacc
    15121 aatatcgggg tcattaaaaa tggacgtcca gcaatcgatt attcaattgt aggtggccac
    15181 ccaacatata tctcttcatt agatgttcgg gttctaggtg tggcaggcgg ttcgatggta
    15241 cgggctaatc agtcaggaat catcgatgtc ggtccacgtt ctgcacatat tgccggtcta
    15301 gattatgcag tatttacgga gacagaaaaa atcaaaggtc ctaaggttga atttttctct
    15361 cctaaagaag gagaccctgc tgattacgtc aaggtagtga tggaagacgg agaagaagtg
    15421 actattacaa atacatgtgc agccaatgtc cttggattag tgcaagaaga acatttttct
    15481 tatggaaatg tcccatctgc acgaaaagcc atccaagcat tagctgatta ttgtcatacg
    15541 acggttgaag acatcgcaga acagatcatg gaaaaatcct atgctaagat tgaaccggtg
    15601 attttagaat tggcagacaa gtatcacttg gagaaagatc agatttctct tgtaggtgta
    15661 gggggcggcg ctgcctcatt gattacttac tttagtaata aaatgggtgt gaaatattcg
    15721 attcctgaaa acgccgaagt gatttcttct attggtgtag cacttgcaat ggtccgagat
    15781 gttgtagaac gtatcatccc ttcgccaagt aaagaagata tacgatcgtt gaaaaatgaa
    15841 gcgatgaata aagcgattga atcaggcgct acaccagaat cgatcgaagt ccacgtagaa
    15901 atcgatccac agacttcaaa agtaacggct atcgctactg gttctacgga agtgaaagca
    15961 accgatctaa ccaaggagat cacaacagaa gaagcactag agcttgctgc agaggatatg
    16021 cgattaaata aaaatgaagt ttgcttattg gaaaatacac ctttctttta tgtatgcggc
    16081 gaacagaatc gttcaaaaaa tgctggaagt cttcggatca tcgatcaaaa aggatttatc
    16141 aaggtccaac gtggacatgc ttcatgtatg aaaacaactg cagccaatta tatgacagct
    16201 gtcgaacaac tttgggaaga tatggcagtt tatcaaactg aattgattgc ccgaccagaa
    16261 ttttatctct gcttaggagc aagagtctct gattttacag cgacggatct ggaacaacta
    16321 cagcttttga tggatctgga agtatctact atggaaccgg aggaagaagt gatcgtcgtt
    16381 gccggaaata tcaaacaaac ataagttaga agagttacta aacgaattac aacagatccc
    16441 cgaagaaatt tggggattct accagtttcg gcgagattta ttttggaaaa agattccttt
    16501 gtccaagcag aaaattctta tacaacaatc aatcgattgt ggtatcgaaa cggcatgtag
    16561 catcaaaaaa aagtaccctt ttgccgatgt aggagaaatt tgtgaacaaa tggctgttcc
    16621 aatcgtttcc tgtgaatcag agcaaataaa cgaacgaatc acttttgcaa cttatgcaga
    16681 agatgaaggg attcgattga tgacggagcc gttagaaaaa ttaaaatgtt caggactagc
    16741 ttcgatctct aaagaaacag cgcaagcttt gattattgga catgaactat ttcaccacat
    16801 cgaagcttct gttaagggaa tttatacgca aagcgaaaaa attgtgctgt ggcggttgcc
    16861 tttttataca catcaatcaa acattcgtgc attgagcgag atagcagcga tgagtttttc
    16921 aaaagagatg aatcaaagcg atttttcgcc ttatgtatta gaggcagtct tactttggcc
    16981 atataatgaa attcgtagcc aagggatctt agaagaaata aaaaaaatag aaaaaagatg
    17041 tacggagtat gatttagatt acaaataaag agactgtgac aaaagtcgtg tcacagtcct
    17101 ttttttccga ataaacgatg ttctcctgtc aactcctcgg tcacaagtca aaatattcaa
    17161 caaacatgag aagctgtttt ttgaaattgt tccttgtgac tcggagatga acggcaggct
    17221 cacgacctct tggaaaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaag
    17281 ttaaaaacat aagaagctat ttttacttgg ctgttcttat tacttgatgc agggcagctt
    17341 tttcacaacc tctcaataaa cggtgtgcaa tcagctgacc ctcggtatta gctcaaaaaa
    17401 aacggcaaaa tatgaggaac tattttgccg tttctttctc taatactcag gtcaaaacgc
    17461 tgattccaaa acctcttatt tgatgggtct gtcctgaata tcttcaaaat aatcggggta
    17521 attatgaatc acgtattctt gctcctcgat aatcatatgt aaatgcatcg tgcaaagaaa
    17581 tccgacttca tcaaagttat gatcaatcat tgattgaagc attgtctgat gttcatctaa
    17641 tactcgaccc cagtctaatt ctgaaatcgt caaacgtaac catctgaaac ggttcaagtg
    17701 cgtactataa ctttctagcc aatcccaaat ttctcgtttt ccagcaattt cataacaagt
    17761 tcggtgaaaa tcagtatcca aatcaaaaaa agctttttta ttttgagcga ttactgcctg
    17821 atcggttttt ccaacttttc ccgcgtataa cgggctaatg aagcagatcg gatatcgatt
    17881 tttgaaacat aagtgccgga ctgtggaatg gtttttacaa gctcttgatt gcgcagttgg
    17941 atcagtgctt ctcgaatcgg tgtacgcccg atagctagtt gttcttctaa cactttatcg
    18001 gagattttac ttccaggtgc taactcggaa taaatgattt gtttacgtat agattcataa
    18061 gcttgctgtt gcaaattatt gataaccatt taaccttcct ttctatgcag atataatcta
    18121 ttataacata aatcgcttac aaatcgtgtg ggcataaaaa cacagtctgc aaaaaaagcc
    18181 ctgataggca tgcctattag agcctcgaaa agaaaaatat tgaatcaatc tggtcgtacc
    18241 tcttgtatcg gtttgacacg accgccgatt cttttagcaa aagcttcgga ttcttttttg
    18301 tctttgaaaa tgagcagatt gtctaagttt ttttcccgag tgcgacagcc agttttatcg
    18361 atgtaaccgt gcattacttt aacgacatac atggggatca cctgcttttt tcttttcatt
    18421 atacgaacta ctcgacacaa tacaagaaaa atgcttagac ggtctttatt aagaatttac
    18481 atcatccaaa gaagcgtacg cttggaataa ctgatctaac tccggattga ctttgctcag
    18541 cttcactaat cgttgcgaat cagaaagtaa aaaacagtgt ttactactgc cagtagtttt
    18601 taattttttg tcttcagttc ccacaatttc gtagctaaaa tccaaccgtg tccccgtata
    18661 cttttggata gttggatgga tgctcacttt ttctccgtaa tggatcattt ctttatagct
    18721 gcaatttact tccagaacgg ggatgatgat cccagattct tcgatttttt tatagggaaa
    18781 ccctagatgt accaataagg ctacccgagc ctcttcaaac cagcggatat aattgctgtg
    18841 atgaataatc cccatttgat ctgtttcata ataaaaaggt tctcgcacat aacctgtaaa
    18901 tttctccatg actcatgcct cgttttccat tgatttttta caaaaataat tcatccattt
    18961 cggaagtgct tcgctgattt gtgtcggcaa aacaatatat tgttcttttg ccagctggtc
    19021 tccaatgaaa ctatgaagat aaatagctgc tgcaatcgtt tggtcatttt gaggaaactg
    19081 ggctaaaaat cctgtgatca tcccagctaa agtgtcaccc atgccgcccg ttgccatagc
    19141 agcagaacct aaaggattgg tgtagaagtg ctggtgacca taaatttttg tctcatgact
    19201 tttcaaaacg agcacagcac cgatttcctg ttgcttcttt ttgttttctt tatcccgttg
    19261 ttcttgtatc ggtagagaac tcaaccgttg ccactccatt tgatgaggtg taagtacagt
    19321 ttgttcggga tagggtagca aaagatgatt agcagcaagt aaacttaaag cagagccatc
    19381 aatcaccagc cattgttctt ttgattggtt ttctagtact tgtttgaaac gttccaaact
    19441 cttagaagaa gtacccaatc cgggaccaat caagagtaca tctgcttgct ttattacgga
    19501 aagaaactgg tcagtatccg tccaaactac aatcattgct tctggcagat gactgtgaag
    19561 agcactccga tttttttcat cagttactac agtcgtgaga cccgctccgc tgtgtacggc
    19621 agctaaagca ctcataatga ttgctccgcc atattgttca ttgccgccaa tcaaaacgat
    19681 acgtccaaat gttcctttat gtgaaatttt tggacgaggt gtgatgactc ttttcactag
    19741 ttgttcgttt atcagcatct aaaaacctcc ttttactcat tatagcgtat atttttttta
    19801 gagaaaaaca gataagaaaa ggaattgtca gattttttga actatttttt tctgtaattt
    19861 gtggtatcat gaacacgaag acttaaataa gtggaggatt aactttatga caattgattg
    19921 gcaaaaagaa gtagaagcac gtaaagaaga cttgcttgaa gacttgaaaa atcttttacg
    19981 tgtcaacagc gagcgcgatg actcaaaagt aacaccagat gcaccatttg gacctggacc
    20041 tcgcgacgca ttgaaacata tgttagctta tggcgaacgt gatggattta aagtaaaaaa
    20101 tgtcgataac tatgcagggc atattgatct tggcgaagga gacgaaacgc tagggatatt
    20161 tggacacatg gatgtcgttc ctgcaggaga tggctgggac acagatcctt atgaaccagt
    20221 aatcaaggat ggaaagatct ttgcacgcgg atcaagcgat gacaaaggtc caagtatggc
    20281 agcttactat gcgatgaaga tcattaaaga acttgacttg aaactatcaa aaaaagttcg
    20341 cttcgtagtc ggcagcgatg aagaaagcgg ctggggcgat atggcttatt atttcgaaca
    20401 tgaagaagaa ccagattttg gtttctcacc agatgctgaa ttcccaatca tcaatggtga
    20461 aaaaggaaat gtttctttag ctttacgctt caaaggcgac aatgcaggag attatgtctt
    20521 gaaatctttt gtttccggac ttcgcgaaaa tatggtgcca ggaacagcaa ctgcagcact
    20581 tcaagtacca agtgcagatg cagctatcgc gatggaagaa gcattttacc aattcatcga
    20641 agcaaatcca gtaagcggaa cgattgaagc ggacaatacg tatgtaaaaa tcgaattagt
    20701 tggtaaaggc gcacatggtg caagcccaca atctggtatc aatgcaggtt ctttcttggc
    20761 attgttctta gataactatg aattcctagg atctgctaaa caatttatcc atgtagcagc
    20821 tgcttatgtc catgaagatt tttatggaga aaaactagga gtcgcttatg aagatgaaaa
    20881 aatgggcaaa ttgacaatga atgctggact atttgctttt gaagaaaatg gtacagagga
    20941 agcgaacttc atcaatatga atttccgttt tccaaaaggt gtaacagtag acggattgca
    21001 aagtgacatc gaacaaacag tggggcaaga aggcgcaaca gttactcgag gtgctcgcgt
    21061 aatggaacca cattacgtac caatggatga tccattggtg gctacattat tgcaagtcta
    21121 tgaagaccac actggagaaa aaggctacga acaaatcatc ggtggcggaa cttacggacg
    21181 tttgttgaaa cgtggcgttg cttatggagc gatgttccca ggatatactg atacaatgca
    21241 tcaagcaaac gaatttatga gtcttgatga cttgttcaga gcaacagcaa tctatgcaga
    21301 tgccatctat cgtttggcga aataattaat taaaaagagg atcaagaatt gcaaattatt
    21361 tttgcagtct tgatcctttt tttactttct actgtggtat tcatgataaa catgatatag
    21421 attcaattca tattctttct tagcgttcgt gacgactgta tccaaaatca accctgttac
    21481 taatgaaaga agtgctaaag tcatcaatcc agtacttaaa atcgcagaag ggatgcgtgt
    21541 aataaagctt gtcagcatat attcgcgaat cacaggaatc cccgtgacca atccaaataa
    21601 aaagaagaag atggtccaaa tcccaaagaa aagcaaaggc ttataatctt taaacatttt
    21661 aataatcatc atgatcactt tgaatccgtc agagaatgta ttcaattttg attcgctgcc
    21721 ttcaggacga tcacgataat cgattgggat ttcaacgaac ttgaattttt tatctaaagc
    21781 atggatcgtc aattccgttt cgatttgaaa accagaactc ataataggaa aacttttgac
    21841 aaagatacga ttgaatcctc gatagccggt catgacatca agaatttttg ttttatacat
    21901 tctagtaatt gtatttctaa caaggttatt tccaaaatcg tggaaaggtc tcttattctc
    21961 atcaaagtag gttccatttg ataggcgatc accaatgacc atatctgctt caccagaacg
    22021 taatttctcg agcaaaccat gtacagcttc tgcaggataa gtatcatcgc catctaccat
    22081 taaataatag tcggcatcga tatcaaagaa catttggcgg atcacatttc cttttccttg
    22141 acgaggttct ttttttacga ttgctccacc ttgaacggcc aattcgtatg ttcgatcggt
    22201 cgaattatta tcatagacat aaatatctgc ttctggtaat tctcgtttaa agtctgcaat
    22261 gactgttgaa attgttgctt cctcattgta acaaggaatc agaacagcaa ttttttcttg
    22321 ttgttccatt tttcactctc cttaactata gaaaacaaaa gctaaattaa aaatacctca
    22381 taatcaaagg aaatgctagt ttttcccgct aaatatcatt tatttaaaaa gaaaatactg
    22441 gaatatatgt ttggaaagct ctatctaaaa aaccacccac tacaaaagaa ggacatgata
    22501 cctctctttt gaatagtggg tgcgtattcg ttctctcact tttttgacgt attaatctgt
    22561 tgcacctttt tttcttgaaa tttctaatac ggttcctgta tgagcatctg ctaaaaattc
    22621 ataataaaca gtttcgccat cttctattcg agaaagacct ccccgataag cactcgaccg
    22681 aacggcgaat ttcctaatag gttccttttc aaaagagatc caagaacctt cgattgcacc
    22741 ttcttttaga aaagatgact tgatattttc aagtacttgg tctgctgaca atactttttt
    22801 gcgatgatac caaatcgttg aagctacccc gctcaataat cctagaccga ttccaactgc
    22861 taacccgcct ttaaaatagc ttagttcgct ttcttcatac atgtggacat acctccttct
    22921 tctcacttgt tactccttgt gcttatttta ccacactctc atattttctt tgaaataaat
    22981 aaccgctacc agaaaaaata agctataata aatacgatcg gtctgcagaa aaaatcatat
    23041 tttatccgca gatcaatata agtctgagag gagtttagtc tgaaatttag acgaatagca
    23101 tatggaagaa aaaacatttc aacgaatcaa agagttaaca gaattacaag gaacaagcgg
    23161 ttttgaacaa gatgttcgcg cttatatgga aagtcatatg gaaccgctgg tagatgagtt
    23221 acagttagat ggtttgggcg gcatttttgg tctgcgccat catgaagaag cggatgcacc
    23281 ccgtgtaatg gtcgcagcac atatggatga agtcggattc atgctgacac aaatccaaga
    23341 aaacgggctt tttaaagtcg tacctctagg cggctggaat ccttacgttg tttctgctca
    23401 gcgttttaca cttaaaacct ctactggtaa gaattatcca tgtatttctt cttccgttcc
    23461 cccacattta ttgcgaggaa caagcggaca aaaatcagta gaagtaaccg atattttgtt
    23521 cgatgctggg tttgaatcaa gagaagaggc aatgagttac ggtgtccttc caggagacac
    23581 aatcgttcct tacgctgaaa cgatcaaaac agccaacgga aagaatatta tcagtaaatc
    23641 atgggacaac cgctatggct gtacaatggt attagaagct ttagaagcgt tgcaaaatga
    23701 aacattaggt catacattga ttgcaggcgc gaatgtccag gaagaagtag gcttgcgtgg
    23761 ttctaaagct tctgtcaata aattcaagcc agatctgttc tttgcagtag actgctctgc
    23821 agcagatgat acagtgacga aaaatggcac attcggacat ttaggagaag gtacattgat
    23881 gcgtatccaa gatccaggtt tgatcatgct tcctcgtttg cgcgagtact tgctggatat
    23941 cgctgaaaca aacaatatcc cttatcaata ctttgtttca aaaggcggta cagatgcagg
    24001 tgctgctcat acacaaaacg aaggaatccc aagtacagtg attggcgtag tgggacgtta
    24061 tatccatacg catcaaacga tgttcagtat tcgcgatttt gaagctgcac gagaaatgtt
    24121 gattcaaaca ctgaaaggtt tggataaatc aacagtgaat acgatcgtat acggaaaata
    24181 atgcgatagt gaaactataa aatctggcaa aagattgctt tttgccagat tttttttata
    24241 aaaagaggat caatgtttgt tagttcgaaa gaaaggtgta taatttaatt gaaaaataca
    24301 taaacagaaa gtagaggaca aagaatgatt atcccaaata gtttagaaga attagcaggc
    24361 tacgtagaaa ctggaaagag tgtatttttc tttactgccg gctggtgcgg agactgccgt
    24421 tttatcaaac cgcaaatgcc tgaaattgaa aatgactttt ctgattggca atttattgaa
    24481 gtagaccgtg acaagtacat agatgtagcc gctgaatgga acatttttgg tatcccaagt
    24541 tttgtcgtga tccaagacgg aaaagaatta ggtcgtctag tgaataaaga tcgtaaaaca
    24601 aaacaggaaa tcgaatcgtt cctagaaagt gtaacaaaag gctgacatct gttaggaggg
    24661 gtcaagatga atcaagaata taagaatatt ttagtaggta tcgacggtag tgaacaagca
    24721 cttgctgctt tcaaaaaagc ggtagaagtg gctcgccgta ataatgggaa agtatatgta
    24781 gcaaacgtaa tcgatcagca attttataac tttatgggct atgcacctat tgatcaaaat
    24841 ctagtcgatc agcaaactga aacagccaag gaaatgatcg aagaatgtaa agaatatggg
    24901 aaatctgttg attacacaaa tatagaagga attattgcat atggttctgt tcgtgaatcc
    24961 atggcagtca aattgccaaa aaaatatgag atcgatctaa tcatggttgg gcagtcaggt
    25021 ctaaatgcag tcgaacgctt tatgacagga agtgtagcca gctacgtgat acgtcattca
    25081 ccttgtgatg tcttgatcgt ttctgacgaa gaagaacagc caagtaatta agcaagtgga
    25141 acttgtacga cctttttaat caattaacta aaacagtaaa gggactgtga caaaagtctt
    25201 gtcacagtcc cttgttacga ataaatggtg ttccagaaga ggcttcttcg gaaataaagc
    25261 atgaaaaaaa tttcatgcag tgaatgcaat tttttgttaa gataaatctc agcaaaggat
    25321 aatggaggaa agaacatgat ttttgcttat aacaaagcac acgtcggaga caccttgatg
    25381 gttattatag ccgacgataa aggaacagaa aacactgttg aacgcaaatg gaatgttgct
    25441 cggatcaaag atgaacaagg tcaagttgtt gcttggaact ttttccatat ctccgatcat
    25501 ctaacaatcg aagggaacgg ccaagtaagc ttaacagatg aggaattagc gaaattgaat
    25561 cgattgatca aagaagcagg attcgaagaa cagcttgaag ctgatcatga accgaagatc
    25621 gttgtcgggt acgtaaaaac atgcgtccca catcctgatt ctgaccattt atcgattact
    25681 gagacagaag tagacaatgg tacagtattg caaatcgttt gcggtgcacc caatatcgaa
    25741 gctggacaaa aagttgtcgt tgcaaaacca ggagctatga tgccagatgg aatgatgatc
    25801 tggccaggaa ctttgcgagg ggtagatagc tatggaatga tctgttccgc tcgtgaattg
    25861 catttgccaa atgctccagc aaaaaaagga atcttagtat tgcctgagga tgccgtagtc
    25921 ggagaagcat ttccaaaata aagaggttgt gacaagtctt tgtcacaacc tcttttttat
    25981 taattttcac tattttgatt ttgttttaat gccgattgat cgatcgtcaa ttcaacagta
    26041 gttgtttttt ctttcgagcc ttcatagtaa gtgattttga cagaatctcc cacttttttc
    26101 ttatacaaag cagattgaag ttctacacca gaagaaactt ctttgtcatc gatcttagta
    26161 atgacgtcgt attgtttcaa tccagctttt tcagccggtg tagctgtttg gacatttgtc
    26221 acgatgacac cgttagttac agatgaagga atcttcaaga tttgttcttg ttgctgagta
    26281 gaaacggcag ataagtcgac cattgtgata cctagagctg gacgtgtcac ttttccgtct
    26341 ttttccagtt gattgatgat attcactaca tcgttacttg gaatagcaaa tcccattcct
    26401 tctacgctta cattagaagt agattcagaa gtacttgcga tcttacttga gttgataccg
    26461 ataacttgtc cttcgatatt aactaaagga ccaccagaat ttccagggtt gattgctgca
    26521 tctgtttgga tcgcattgat attgactgtt tcgttcgatt cattcgtact tgtcacttgt
    26581 cggttcaagg aagaaatgat tcctgaagtg acagaatttg catattcaga tcctagtggg
    26641 gaaccaatcg caatcgcagg ctcaccaacc ttcagagcac ttgagtcacc aaaagaggca
    26701 actgtttcga ctttgtctga attgatcttc aacaccgcta aatctgaata agcatctgtt
    26761 ccaactaatt cagcttttac cttcgtaccg tctttcatca agacttcaag accttgctgg
    26821 ccatctacta cgtgattatt cgtgactaca taagcagtat tcccgtcttt tttgtagatc
    26881 acgccgcttc cttcactata agcttccaaa ttactatcat ctgagctgct actttcttct
    26941 tgctgcccga atagtccgcc aaaaccgctt gattggtttt gactttgtaa gttgataaca
    27001 gaaacgacag cgtcttgaac tttatctacc gctttggtga tatcagaatc tacattgact
    27061 ttgacatttt ccacaactgt ttcaccggca gaattctgat tgccgcttgt agcagtagaa
    27121 ctattgttgc cagtgcccat cgctgcataa aaaatgccag ctgtcagtag tcctccaacg
    27181 atccctccaa caagccccag acctaactta cgccagaggc tattattttt actttttttc
    27241 atctttggtg tcacattttt tctatccatg tgttcctcct ccaattacat tcatttatct
    27301 actgttagta tagtcttatt tgttgacttt agtctacttt gagactgtga actttttatg
    27361 aaaaacttta ggaaaaatca ttgttttcag atgaaaatat ttgattttct ttttccacag
    27421 atactgtgta aaactacgcg gataggattg gaaaaataag ggataaaaca tatccacagc
    27481 ctgtgtataa agtggataaa ttcttataaa acgtatgttt gcctaatgaa tgtgatgaaa
    27541 taaggtaaaa taacactgtt ttctgtggat aaacctgtgg atgttgtgca taactgttgg
    27601 ggaaatcttt ttaacaaaaa tagccaaaat aaacatatag acgaaaaaat tgattataat
    27661 aaacaaagtg agcatgaaga ccaaaggaga gaaaacatga atatcaaaat tatctcagta
    27721 ggaaaattaa aagaaaaata cctgatccaa ggtatcaatg agtatgtcaa acggctgaat
    27781 gcctatgcaa aaatcgaatt gatcgaagtt cctgacgaaa aagccccgga gaatttaagc
    27841 gaagcgcaaa tgagacaagt aaaagaaaaa gaaggggaac ggatcttagc gaaaatcaaa
    27901 gaacaagaat acgtctatgc cttagcaatt gaagggaaaa atcctacaag tgaatctttt
    27961 gctaaacaaa tcgaccaatt aggtatccaa ggaaaaagcc accttgtttt tgttatcggc
    28021 ggctctttag gactcagcga agccgtcatg aaaagaagca atgctcagat ctcttttgga
    28081 aaaatgacgt atccacatca attgatgcgt ctgatccttg tcgagcaaat ctaccgtgcc
    28141 ttccggatca atgcgggggc gccttatcat aagtgatgcg gtttttatca ttgatcctaa
    28201 aaatcttagc aagacaaaaa gtacgtaaga aagtaaagac taaatacttc ggaggaacaa
    28261 atgaataaca atgataggtt attgcgattg cgttatgcga ttgatttgaa agatacggat
    28321 ttgattaaag cttttgagct aggcggtgta ccactgacta aagaagaagc tcaggcggta
    28381 ctgaccaaag tacaggataa gaacaaagat aatgcagaaa ataatgtgta tgaaaaaacc
    28441 atcaacaacc aagtttttga cgcattccta aatggcctga ttctcttggc gcgtggtccg
    28501 caaaaagata agccgatagt agacacgccg caaaaaagca aggaaatcaa atatatcaac
    28561 aatgtgttgc taaaaaaatt aaaaatcgcc ttgtctatga ccactgatga tattttagac
    28621 gtttttgctg aggcagaaat ctatccatca aaaggcgaaa tcggggcatt tttgagaaaa
    28681 gaaggccaac gaaattttaa gccttgcgga gataaatata tgcgtaattt tctcaagggg
    28741 ttaggaattt ataatcggag aaaagtataa agaaatttca tgagaaatca aaacaatggt
    28801 tgttttgatg agagctagtt tttgtatgag attaaaatat tatcttaatc gaggagctgg
    28861 attggtttta tgaatgatga atataagaat gatgaagata agatgctttt tgaagagata
    28921 gagaatcgat gtaggctcaa ttttgagctt cggggaaaga tgtctttaat acaacaaaaa
    28981 aagtatttag cgaataaatc agagtttact ctgggacatg ttgaaaaatt aatctcggat
    29041 tggattagta gtagaagcga atttacaaaa ataaaacagc cgattaaatt tgacatgaaa
    29101 aaacttttgt taaataagag tgagataggt aatagagatc aatatattag agctaaaggg
    29161 caagaaataa ttgattcatt aggtgaaatg aggtcataca attatttgta tgtaacacat
    29221 agagctgatg gaatggtcat tactgtgggg aaatcttctt ctaatgatat ttttttagat
    29281 ggagatcttt tttatcaact caatacaaat cacttgtctg gcactgaaaa tataatttta
    29341 aggacggaat atggcaatga aatatttgca aaatatgatg agattttaaa aaattatttg
    29401 gactgggctt ggataattcc ggttgagtca ggagatgcta agaaactgga gagattattg
    29461 ggcgatgaac taattaataa aaaagtacca attt
//
LOCUS       DAJSKS010000018        27846 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJSKS010000018 DAJSKS010000000
VERSION     DAJSKS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.018"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            191..1450
                     /gene="lysA"
                     /locus_tag="ON396_000731"
     CDS             191..1450
                     /gene="lysA"
                     /locus_tag="ON396_000731"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR3112859.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1437..2237
                     /gene="dapF"
                     /locus_tag="ON396_000732"
     CDS             1437..2237
                     /gene="dapF"
                     /locus_tag="ON396_000732"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR3112860.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2240..3121
                     /locus_tag="ON396_000733"
     CDS             2240..3121
                     /locus_tag="ON396_000733"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR3112861.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3118..3834
                     /locus_tag="ON396_000734"
     CDS             3118..3834
                     /locus_tag="ON396_000734"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR3112862.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3840..4535
                     /gene="dapD"
                     /locus_tag="ON396_000735"
     CDS             3840..4535
                     /gene="dapD"
                     /locus_tag="ON396_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCR3112863.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4551..5552
                     /locus_tag="ON396_000736"
     CDS             4551..5552
                     /locus_tag="ON396_000736"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR3112864.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5549..6910
                     /locus_tag="ON396_000737"
     CDS             5549..6910
                     /locus_tag="ON396_000737"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCR3112865.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6870..8021
                     /locus_tag="ON396_000738"
     CDS             6870..8021
                     /locus_tag="ON396_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCR3112866.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7996..9165
                     /locus_tag="ON396_000739"
     CDS             7996..9165
                     /locus_tag="ON396_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR3112867.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9340..10719
                     /locus_tag="ON396_000740"
     CDS             9340..10719
                     /locus_tag="ON396_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3112868.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            11000..11797
                     /locus_tag="ON396_000741"
                     /pseudo
     CDS             11000..11797
                     /locus_tag="ON396_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11986..12654
                     /locus_tag="ON396_000742"
     CDS             11986..12654
                     /locus_tag="ON396_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCR3112869.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(12854..13084)
                     /locus_tag="ON396_000743"
     CDS             complement(12854..13084)
                     /locus_tag="ON396_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112870.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(13211..14593)
                     /locus_tag="ON396_000744"
     CDS             complement(13211..14593)
                     /locus_tag="ON396_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR3112871.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(14749..15936)
                     /locus_tag="ON396_000745"
     CDS             complement(14749..15936)
                     /locus_tag="ON396_000745"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR3112872.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(15943..16974)
                     /locus_tag="ON396_000746"
     CDS             complement(15943..16974)
                     /locus_tag="ON396_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR3112873.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(17020..18444)
                     /locus_tag="ON396_000747"
     CDS             complement(17020..18444)
                     /locus_tag="ON396_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3112874.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            18619..19524
                     /locus_tag="ON396_000748"
     CDS             18619..19524
                     /locus_tag="ON396_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR3112875.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            19615..20292
                     /locus_tag="ON396_000749"
     CDS             19615..20292
                     /locus_tag="ON396_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR3112876.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            20289..20459
                     /locus_tag="ON396_000750"
     CDS             20289..20459
                     /locus_tag="ON396_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112877.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            20573..21469
                     /locus_tag="ON396_000751"
     CDS             20573..21469
                     /locus_tag="ON396_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3112878.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21524..22894)
                     /locus_tag="ON396_000752"
     CDS             complement(21524..22894)
                     /locus_tag="ON396_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3112879.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            23132..23878
                     /locus_tag="ON396_000753"
     CDS             23132..23878
                     /locus_tag="ON396_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR3112880.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            23875..24714
                     /locus_tag="ON396_000754"
     CDS             23875..24714
                     /locus_tag="ON396_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR3112881.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            24715..25665
                     /locus_tag="ON396_000755"
     CDS             24715..25665
                     /locus_tag="ON396_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCR3112882.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            25806..26558
                     /locus_tag="ON396_000756"
     CDS             25806..26558
                     /locus_tag="ON396_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112883.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            26577..27728
                     /gene="queG"
                     /locus_tag="ON396_000757"
     CDS             26577..27728
                     /gene="queG"
                     /locus_tag="ON396_000757"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR3112884.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         9144 a         5051 c         5840 g         7811 t
ORIGIN      
        1 gtataaatag cttctaatag ttcgggtatt ttgtttttat aaccattttt attcttattt
       61 ttaaaaaaat atttcgaaat atactagaca attcagaaaa ttttgtttaa tattattcca
      121 ataaaattca aatctttatc aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag
      181 gaatgaaaaa atgacagaat tgataattgg cggagtcaca gcaagcacat tggcagaaaa
      241 gtataagaca cctctgtatg tgtatgatga agaaaagatg agacaaacga tgctgtcatt
      301 caagcgaggc tttgtatcga aacgttttga tacaaaagtg ctttacgcct ctaaagcatt
      361 ccaaacgatt gagatgctgc atttagttcg tgaatatgga ttcgggctag atgttgtcag
      421 tggtggggaa atctataccg ctttgcaagc tgattttcct agagatacta tctacttcca
      481 tggtaacaac aagacgccag aggaactgat ctatgcactt gaaaatgatt tgttgcatat
      541 tgttgccgat aatttgatgg aagttgaatt attagcagaa ttaagccaac gctatttaaa
      601 aaaaatctgt atcatgcttc ggttgaatgt cggagtagaa gcacatactc atgaatatat
      661 cgtcactgcg catattgatt caaaattcgg catgagctat gaaagtaaag agtgccaaca
      721 agcccttcag ttgataaaag aaagcagcta tctagagtta gaaggatttc atgctcatat
      781 cgggtcacaa atttttgatg ttactgcttg gttagctgaa atcgacaaat tgacgaatta
      841 cttaaccgat tttgaccaac ctctttcctt gaatcttggc ggagggtttg ggattcgtta
      901 tacacaaaat gatcagccga tcccagtcga agaggcacta aaaaaactga ttgaatatac
      961 ggaagaagct ttggaaaaac gaaaactgac cattcgtcaa ttactgatcg aaccgggtag
     1021 aagtattgtt ggagaagctg gtacaacact atacacgatt ggtttcatca aaaagacacc
     1081 acataaaaaa tattactttg tagatggcgg gatgacagat aatatccgtc cagctctgta
     1141 tcaggcagaa tacgattgtg atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt
     1201 gacgattgca ggaaaaatgt gtgaatcagg cgatgtggta attaaagaag cttatttgcc
     1261 gaaagcagaa tcgggtgatc ttttagctgt ttattccacg ggagcttatg gatactcgat
     1321 gagcagcaac tacaatcgag cgacacgacc agcagttgtt tttgtcaata atggccaatc
     1381 aagactgatt gttcgcagac aagcattttc tgatctacta aggggggaag tttcctatga
     1441 tccttcataa atataatggc tgcggaaatg attttatctt attagattac gcagaagaca
     1501 cagattatag tcaattagcc gcttctttat gcacaaaaga aaaatatgat atagacggat
     1561 tgatcgccgt aaaaatcgat ccgttggaaa tgatctatta caacaaagat ggcagccgcg
     1621 cgccgatgtg cggaaatggc attcgctgtt ttgctcgata cgtaaatgat cagaacatga
     1681 tgaatgaaca ggaattcaac gtagagacat tagcaggcac aagaaaaatt acggtgttgg
     1741 atccctcacc tttttattgc agcgtagata tgggagtgcc agatgattct ccggaaaaaa
     1801 tcaaagctaa tcaaacttat ccaataaaaa accaggaaat agagatcaat ggtgaaaaag
     1861 taacgatcac tagtttattt ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc
     1921 gtgaggaagt ctggagaaaa agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa
     1981 aaaccaatgt caactttgta gaagtagtca atgaacaaga attgatcgtc cgtacctatg
     2041 aaagaggagt aggttggaca cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg
     2101 ctaaggaaac tgggaaaatt tcagcagatc aggtagttgt tcacttggaa caaggagact
     2161 tgatgatctc tggtgaggat gcgatacaaa tggctggccc agcagttcat gaatggacaa
     2221 gaatattgga ggaatgaaga tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa
     2281 tgaagaggga tcagtagatt atgctggttt ggaaaaattg atccaattcc atttagatga
     2341 acaaacagat ggattattag ttttaggtac aacaggagaa tcttctacgc tcacacaatc
     2401 tgaggaggag cagatccttc aactgacagt aaagaaagtg gctggccgtg tgccggtgat
     2461 tgcaggagct ggaacgaata atacgaaaga aacgattgaa aaagcgaagc attttgcttc
     2521 tttgggggca gacgcacttt tggtcatcac cccttactac aacaaaacaa gtgatgcagg
     2581 actagctgct cattttacag caattgcaga agcctcaccg attccgctta ttttgtataa
     2641 tgttccttct agaacaggca tgtcgattcc aatccatgta ttagtaaatt tagcagaaca
     2701 tcctaatatc attggtttga aagaagcctc aggagatatg gcttatgtga tggatgccgc
     2761 tcggttgatt ggagaagaat tcttccttta cagtggaaat gatgatttga tcctaccagt
     2821 aatgagtgta ggaggaagcg gtgtaataag tgtatgggcg aatatccaac ctaaaatagt
     2881 ccatgaatta gtaaaagata cgcaggacgg aagatggcag caagcaaaag aaaagcaatt
     2941 aaatgcactt gaactgatcc atgccttgtt cagtgagacc aatcctattc ctgtcaaagc
     3001 agccatgtca ttattagact taccttccgg acctctacgc ctgcctttag tttctctttc
     3061 aaaagagaaa aagaaacaat tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg
     3121 aaaatcggac tgattggcag tggacagatg ggaaatagag tcgaagaagt tgcacaagaa
     3181 agaggagata cggtccttct tttacggacg gcacctaaag aagcaactac gcttgctttt
     3241 acggaactgc cagaactgct tattgatttt tctcatccag ataatttaga gaaaatcctt
     3301 tcgtatagct tatcatatca acttccgtta gtcattggca cgacaggtta tacacaatca
     3361 cagttccaag aaataaaaga acatgccaaa catgtacctg tgatgtatag tgctaatttt
     3421 tcgttcggca tcatggtgat gaatcaacta atcaagcaag cagctgcaat gttgcaaggc
     3481 tggcagatcg aactgattga aaaacatcat agtcgtaaaa aagatgcacc atctggtact
     3541 gcaaatatgc tgcttcaaac aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg
     3601 caagggaaaa aaagagaaga aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta
     3661 cctggtgaac atgatgtatt gtttgctgct actgatgaaa tatttacgat caaacatgaa
     3721 tctttttcta atcgaatctt tgctgcaggc gcagtagaag cagcggactg gttgaaagat
     3781 cagtcaccag gttattacaa attagaagat ttattaatgg acaaaggggt gtaaaaaacg
     3841 tggaagctca agaaatcatc cgctacattc aaacggcatc taaaaagacg cctgtgaaag
     3901 tctatctgaa tgtaaccgag ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa
     3961 gcagcttcgt tgtttttggt gactatgaga cgattgctcc ggttttagaa gctgaatcag
     4021 aaaaaatcat tgaaatcgaa atcgaaactg ctgcaagata cagcgcagtt cctttgctag
     4081 atattaaaaa aattaatgct cgtatcgaac caggtgctat tatcagagat caagtgtcaa
     4141 tcggcaacaa tgccgtgatc atgatgggag cgattatcaa tattggtgca gtaatcggtg
     4201 aaaacacgat gattgatatg ggtgctgtct taggtggacg agcgacagtg ggcaaaaact
     4261 gccatattgg tgcaggagct gtcttagcag gtgtgatcga accagcatcg gccaaaccgg
     4321 ttatcgtaga agacggcgta ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata
     4381 tcggaaaaga tgcagtcgtg gcagcaggag cagtggtttt agaagatgtg gcagcagaaa
     4441 ctgttgtcgg tggaatacct gcacgtgtat taaaaataag tgatgatcag acaaaagaaa
     4501 atacagcatt gattgctgcg ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa
     4561 tagatacctg tattgtgggg gcaaccggat tagtcggcca aacgatgctc aaagtgttag
     4621 aagaatatga ttttccagta ggacgattga aattattagc ttcaaaacgt tcagccggta
     4681 aagtttgtgt attcaagaaa aagaaacaca ctgtacagga attgacagaa acttcatttg
     4741 aaggatatga tttagcgcta ttttctgcag gtgcttctgt atccaaagag tttgcgccaa
     4801 tcgcacgaag tcagggacta tttgtcatag ataattcctc agcatggcgg gaagattcgg
     4861 atattccatt aatcgttcca gaggtcaatc tggaagacta tacattgaat cggctgattg
     4921 caaatccaaa ttgttcaacc attcaagcgg tacttccgct taaagcttta caagaagcct
     4981 ttggtatcca gcgagtcaat tattcgacct atcaagcagt ttctggttcc gggcaaaaag
     5041 gcattgaaga tcttcatcgt acgcaaaatg acgaggaacc agttttgtat ccttataata
     5101 ttagccaaac ggtgattccg gaaatcgatc agcctttgga aaatgggtat acaaaagaag
     5161 aacagaaaat gataaatgag acccgtaaga ttctgcatca gcctgctttg ccagtatcag
     5221 caacctgcgt acgtgtgcca atcgaaaatg gacatggagt ctctgtggca gttcaactgc
     5281 aaaaagagtt tacgacggaa gaaattagag agtgtctcag ccagtttcca gggattcaat
     5341 tagaagatga tctagcagca cataaatatc cgacgagcat tcttgctaga ggaacagata
     5401 tggtttatgt tgggaggatc agaaaagaca cttctttaga gaacggattg ttgttctata
     5461 cgactgccga taacatacgc aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt
     5521 taatgcagaa aatagcggag gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg
     5581 cggaacatcg atgtctcatg aagagcaatt caaaaaagtc cgtacaattg ttctttcaga
     5641 tcccaatcgc caagtgatcg ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa
     5701 agtaacagac ttactttatt tgatttatgc ttaccttcag catcatgttg attggacacc
     5761 cctttggaaa aggatttgtg atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt
     5821 acctattgaa gatgaactag cacgcataca gaaacaactg gaagacgaaa agataactga
     5881 agattattta gtaagtagag gtgaatatct gactgctcaa ctgatggctg agtatcttgg
     5941 ttaccagttt attgacgcaa aagacctgat tttctttgac tatgatggga aaatcgatca
     6001 ggaaaaaaca gcacaggaaa tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc
     6061 agggttttac ggggcgaacc ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga
     6121 cattaccggc gcgatactgg cgaagatgtt gcaagcggaa agttatgaga attggacgga
     6181 tgtttcagga atcatgatgg ctgatccgcg aatcatcgat catcctaaaa tcatcgagga
     6241 aatctctttt agagaactga gagagatggc ttatatgggg gctaacgttc ttcatgaagc
     6301 agctgttttc ccagttcaag aggctgatat ccccatccaa ataaaaaata ccaatgatcc
     6361 tttcgctcaa gggacaaaaa tatcgaatca cgaaatagaa aaggaaaatg gactgacagg
     6421 aatagctgga cgcaaggatt ttctttccat cactttattc aaacgtcata tgtccgatga
     6481 aatcgggttt atctggaaag caatgagcat ttttgctaaa catgagatat caatcgaaca
     6541 tattccttca gggatcgaca atatcggagt gatcgtctca gcagaagcga ttgccgataa
     6601 gttatttctc atcacgaaag aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat
     6661 cgaagactta gctttgatct ctgtcgttgg gggtccgcat aaagaattga ttggattatc
     6721 tgggaaagtg ttatcgatat tgaacaaatt agaaattcgc acatcgatct tatcacaagg
     6781 agcacaggag ttgaatctga tcatcggtgt accgaataat caatatgaaa cagtagtgaa
     6841 gggtatctat gaagggatgg tgaatacgga tgctgaaaga atatcgaatg gcactccatc
     6901 aaattcctga aatcgggttt catgaattca agacaaaaaa atatttatat gaacaagtaa
     6961 aaaacagcga ggccgttatc catgaaatcg gcgaaacagg actactcctt tattttgata
     7021 atcagcaaga agaaacgatt gcttttcgaa cagatatcga tgcactgcca gtcaaagaag
     7081 aaacgaatct ctcttttgct tctaagcatg aaggttatat gcatgcatgt ggtcatgacg
     7141 gacatatggc gatgctttta gggttgactg actttataac taaacacatt gaggatctta
     7201 tttataatgt agtactgatc tatcagccat cagaagaaat ttccggtggc gcacaaagcg
     7261 ttattcagtc aggattgttg gataaatatg aagtacaagc aatatttggc tttcatctat
     7321 ggcctggact agctaaggga gaagttttct ctcgaccagg acctttgatg gcacaaagca
     7381 gtgaaacaga cattatcatt aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa
     7441 tcgatagtct ggaggcagct gtacgatttt tgaagaatgt ttatgatttt gaccggcaat
     7501 taccggcaga gtggaatcat ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa
     7561 atgttttagc tggagaagtt attgtatccg gaagtatccg ttcttacagt tcagatatgc
     7621 aaaattattt gaagagggga ttagaccatt tagcagaaca gtttcactct acagctacta
     7681 tttcttttcg ctataatgac ggctatccag ctgtaatcaa ccatagaggc ttatatgcag
     7741 cactgttaga ttcaaacaaa ctacatgaat tagatgctcc agttcttcaa gccgaagatt
     7801 ttggcatata tacgcaacaa tatccatgtg tatttttctt tttaggtgtc ggtgataccc
     7861 ctgctttaca tgatccgacg ttcgattttg atatggatgt tttagaaaat ggtgtagaat
     7921 ggtacaaaac gattcttttt acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag
     7981 aggagcgagt aaataatgat gaatttatcc aatcgattta gtcctaggct ttctagaata
     8041 gaagtatcaa aaatacgaca attcgatcag caaatatcgt cgataccaga tgtgatcaaa
     8101 ctcaccctgg gagaaccgga ttttccaact cctgaacatg tcaagcaagc aggaatagca
     8161 gcgattgaag aagacttttc tcactatacc ggaatgagag gattagagga actgcgggaa
     8221 gcggcttgta tatttcagca acagcgttat ggattaactt atgatccgca aacggaagtt
     8281 ttgacgacag ttggtgcaac ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa
     8341 ggagacaagg ttcttattcc agcgcctgct tattcaggat atcagccatt agtggaacta
     8401 gctggtgctg aattgatccc gatagatact tctgacacag ggtttgtctg tcagccggaa
     8461 caatttgaac gagcatttga acaatatgga tcagctgtca aagcagtcat tttaaactat
     8521 cctaacaatc ctactgggac gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt
     8581 ttaaaaaaat atccggtatt tgttataagt gatgaagtat atgctgaatt gacttatagt
     8641 ggaacgcata tgtccatagc tacttatctt ccagaacaaa caatcgtgat aagcgggtta
     8701 tccaagtcac atgcgatgac tggttggcga gtgggcttta tttttgctca aaaagccctt
     8761 attgacgaac taattaaagt ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa
     8821 aaagctgcgg ctgaagcgct aataaatggg gtgaaagata gcgagaatat gaaagagcaa
     8881 tatcaaacac gaagagacta tctggtccag caattggcgc ctttaggatt tgaagtgacc
     8941 cagccaaacg gtgcatttta cttgttttgc aaactcccag ctgccattca aactgactct
     9001 tggcagtttt gtgtagaatt agcggaacag gcaaaagttg cgtgtatccc aggaattgca
     9061 tttggcccag aaggagaagg ttatatccgg atttcttatg ctagcagcat ggaaaaatta
     9121 catgaggcct gcaaacggat caaagacttt ttaacagaac agtgattcat agattttatc
     9181 aaaaaaacaa tcgttaattt tgcggtatat tctgcaagga gacgattgtt ttttaaattt
     9241 agttcaaatg aacgcgtttc ttttgattca taactaatgt aatgttaagt ttaaattgtt
     9301 tagaatgatt ttaaaagaat attcaaggag gtttcatcaa tggaaaaaat caaagttgtc
     9361 attgtaggtg catctcatgg agggcatcaa tcgatcttgg aactattgac acgatacggt
     9421 gaaaatgtag atatcacgct atttgaagca ggagactata tttccttcat gtcatgtggt
     9481 atggagctgt atttagaaga caaggtaaca gatgtaaatg atgtacgtaa tttcagacca
     9541 gaaaactttc cgcaatccaa tgttcatatt ttaaataatc acgaagtaaa aacgatcgat
     9601 gccgataaaa aaacagtaac ggttactagc gcatcagata acgaaacaaa agaatatcct
     9661 tatgacaaat tgattttgag ttctggtgtc aaaccaaaca atctgccagt accaggagca
     9721 gatctagaaa atgtctactt gatgcgtgga tacaattggg caaccaaaat caaagaaaaa
     9781 ttaaatgatc cggctattaa aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa
     9841 gcagcagaag caagccgaaa agctgggaaa gaagtcgtat tgcttgatgt gatcgataga
     9901 cctttaggca cttatttaga tgctgagatg acagatattt tagaacaaca cctaaatgaa
     9961 aacggtattg aagttttgac aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt
    10021 gaggcaatca aaacaagtga aaaagaaatc ccagcagatc tagtcattca agctgccggt
    10081 gtgaaggcaa atacagaatg gttaaaaggg atcgtcgact tagatgaacg tggctggatc
    10141 caaacaaatg aatacttgca aacgaatctt ccagatgtgt atgcagtagg agatgctaca
    10201 cttgcttatt ctatcccagc acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt
    10261 agagaagcac gttatgtagt aaaacatctt tttgaagaag taccaaacaa accgttcggc
    10321 ggagtcgtag gttcctctgc attaagtgtc tttgactatc attttgcagc aagcggattg
    10381 aatagcttta cagcagcaaa agcaggagta gatgtacgag tgtcttatta tgaagatacg
    10441 ttacgaccaa aatatgtacc agaagaacaa ggaaatccaa aagtgtccat ccaattattc
    10501 tcggatccaa tgacccatca attgctagga ggagcagtat tatctaccta tgatgttaca
    10561 ggtcaaggaa atgtattggc actagcgatc caaaacaaac tgactttaga agaactagca
    10621 gaagctgact tcttcttcca gccaggcttt gaccgacaat ggagcatgtt gaatctagcc
    10681 gctcaacaag cgttaggaga agaaccattt gtagaataag aaaacgaaat atttgaaaag
    10741 aggttgtgaa aaagctgcct tgcatcaagt aataagaaca gccaagtaaa aacagcttct
    10801 tatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10861 ttcaaaaaag ggactgtgac acgatttgtg taccgacccc caaaagttag accaaaaaat
    10921 ctaacttttg gaggtcggta ttttttatgg ttaaatatac ttttgaactt aaattaaaga
    10981 tcgttcatga ttatttggat ggcaaaggcg attggaccgt aaaaatccag atcaagagat
    11041 tgaagcggaa atgcttaaaa ttcgggagaa gcacaaggat tatggctgtt tacggatgac
    11101 caatgaacta agaaaccgcg gtttctctat caataaaaag aaagttcagc gcttgatcaa
    11161 gaagctagga ataaaagtaa cggcatacac gcgtaaatca cgtcgctaca attcttatcg
    11221 aggaaaagtt ggagcagtag ccaaaaatcg gatccatcgt cgtttctaca ccagtatctg
    11281 ccatcagaaa atcacgacgg atacaactga atttaaatac catgaagtgg atgccaaagg
    11341 cattatccgt caaaagaaat tgtatctaga tccttttatg gatatgttca atagtgagat
    11401 tttatcctat cgtatctctg aaaagcccaa tgcattagcg gtaatggaag gtttggagga
    11461 agctatccag accactaacg attgtcccta tcgtcgtacg tttcactcag atcaaggttg
    11521 ggcgtatcaa atgaaagctt atagaaacaa attgaaggag tacaaaatct ttcaaagcat
    11581 gtctcggaaa gggaactgct tagataattc acttatggag aatttttttg gcttattaaa
    11641 acaagagatt tttcacggga aagtttataa ctgtttcgag gaattaaaat cagccatcga
    11701 tagctatatc tattactaca ataatgaacg gataaaacag aaattaaact ggcaaagtct
    11761 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
    11821 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
    11881 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
    11941 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
    12001 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
    12061 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
    12121 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
    12181 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
    12241 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
    12301 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
    12361 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
    12421 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
    12481 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
    12541 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
    12601 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
    12661 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
    12721 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
    12781 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
    12841 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
    12901 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
    12961 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
    13021 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
    13081 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
    13141 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
    13201 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
    13261 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
    13321 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
    13381 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
    13441 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
    13501 ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg
    13561 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
    13621 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
    13681 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
    13741 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
    13801 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
    13861 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
    13921 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
    13981 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
    14041 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
    14101 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
    14161 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
    14221 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
    14281 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
    14341 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
    14401 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
    14461 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
    14521 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
    14581 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
    14641 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
    14701 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
    14761 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
    14821 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
    14881 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
    14941 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
    15001 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
    15061 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
    15121 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
    15181 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
    15241 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
    15301 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
    15361 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
    15421 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
    15481 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
    15541 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
    15601 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
    15661 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
    15721 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
    15781 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
    15841 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
    15901 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
    15961 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
    16021 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
    16081 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
    16141 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
    16201 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
    16261 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
    16321 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
    16381 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
    16441 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
    16501 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
    16561 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
    16621 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
    16681 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
    16741 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
    16801 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
    16861 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
    16921 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
    16981 gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc
    17041 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
    17101 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
    17161 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
    17221 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
    17281 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
    17341 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
    17401 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
    17461 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
    17521 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
    17581 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
    17641 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
    17701 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
    17761 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
    17821 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
    17881 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
    17941 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
    18001 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
    18061 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
    18121 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
    18181 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
    18241 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
    18301 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
    18361 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
    18421 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
    18481 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
    18541 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
    18601 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
    18661 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
    18721 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
    18781 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
    18841 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
    18901 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
    18961 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
    19021 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
    19081 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
    19141 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
    19201 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
    19261 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
    19321 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
    19381 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
    19441 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
    19501 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
    19561 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
    19621 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
    19681 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
    19741 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
    19801 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
    19861 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
    19921 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
    19981 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
    20041 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
    20101 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
    20161 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
    20221 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
    20281 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
    20341 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
    20401 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
    20461 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
    20521 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
    20581 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
    20641 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
    20701 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
    20761 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
    20821 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
    20881 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
    20941 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
    21001 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
    21061 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
    21121 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
    21181 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
    21241 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
    21301 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
    21361 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
    21421 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
    21481 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
    21541 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
    21601 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
    21661 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
    21721 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    21781 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    21841 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    21901 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    21961 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    22021 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    22081 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    22141 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    22201 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    22261 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    22321 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    22381 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    22441 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    22501 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    22561 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    22621 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    22681 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    22741 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    22801 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    22861 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    22921 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    22981 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    23041 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    23101 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    23161 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    23221 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    23281 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    23341 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    23401 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    23461 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    23521 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    23581 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    23641 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    23701 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    23761 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    23821 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    23881 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    23941 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    24001 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    24061 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    24121 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    24181 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    24241 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    24301 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    24361 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    24421 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    24481 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    24541 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    24601 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    24661 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    24721 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    24781 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    24841 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    24901 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    24961 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    25021 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    25081 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    25141 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    25201 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    25261 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    25321 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    25381 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    25441 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    25501 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    25561 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    25621 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    25681 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    25741 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    25801 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    25861 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    25921 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    25981 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    26041 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    26101 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    26161 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    26221 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    26281 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    26341 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    26401 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    26461 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    26521 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    26581 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    26641 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    26701 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    26761 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    26821 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    26881 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    26941 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    27001 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    27061 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    27121 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    27181 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    27241 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    27301 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    27361 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    27421 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    27481 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    27541 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    27601 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    27661 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    27721 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
    27781 actggttttt ttgtattttt tacatttttt taagagaatt tgataaaaat caaaaatttt
    27841 tttgct
//
LOCUS       DAJSKS010000019        27716 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJSKS010000019 DAJSKS010000000
VERSION     DAJSKS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.019"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(248..2140)
                     /locus_tag="ON396_000758"
     CDS             complement(248..2140)
                     /locus_tag="ON396_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3112885.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2137..3864)
                     /locus_tag="ON396_000759"
     CDS             complement(2137..3864)
                     /locus_tag="ON396_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3112886.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3880..4530)
                     /locus_tag="ON396_000760"
     CDS             complement(3880..4530)
                     /locus_tag="ON396_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR3112887.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4734..5030
                     /locus_tag="ON396_000761"
     CDS             4734..5030
                     /locus_tag="ON396_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR3112888.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5202..5822
                     /locus_tag="ON396_000762"
     CDS             5202..5822
                     /locus_tag="ON396_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR3112889.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5914..7080)
                     /locus_tag="ON396_000763"
     CDS             complement(5914..7080)
                     /locus_tag="ON396_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3112890.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7092..7445)
                     /locus_tag="ON396_000764"
     CDS             complement(7092..7445)
                     /locus_tag="ON396_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR3112891.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7573..8595
                     /locus_tag="ON396_000765"
     CDS             7573..8595
                     /locus_tag="ON396_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCR3112892.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8614..8775
                     /locus_tag="ON396_000766"
     CDS             8614..8775
                     /locus_tag="ON396_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112893.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9034..9468)
                     /locus_tag="ON396_000767"
     CDS             complement(9034..9468)
                     /locus_tag="ON396_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112894.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9578..11437)
                     /locus_tag="ON396_000768"
     CDS             complement(9578..11437)
                     /locus_tag="ON396_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR3112895.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11555..12715)
                     /locus_tag="ON396_000769"
     CDS             complement(11555..12715)
                     /locus_tag="ON396_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HCR3112896.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13022..14446)
                     /gene="araA"
                     /locus_tag="ON396_000770"
     CDS             complement(13022..14446)
                     /gene="araA"
                     /locus_tag="ON396_000770"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR3112897.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14462..15160)
                     /locus_tag="ON396_000771"
     CDS             complement(14462..15160)
                     /locus_tag="ON396_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR3112898.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15117..16778)
                     /locus_tag="ON396_000772"
     CDS             complement(15117..16778)
                     /locus_tag="ON396_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HCR3112899.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVSGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16996..18396
                     /locus_tag="ON396_000773"
     CDS             16996..18396
                     /locus_tag="ON396_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCR3112900.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18648..19727)
                     /locus_tag="ON396_000774"
     CDS             complement(18648..19727)
                     /locus_tag="ON396_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3112901.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19813..20772)
                     /locus_tag="ON396_000775"
     CDS             complement(19813..20772)
                     /locus_tag="ON396_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCR3112902.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20784..22295)
                     /locus_tag="ON396_000776"
     CDS             complement(20784..22295)
                     /locus_tag="ON396_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCR3112903.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22317..22880)
                     /locus_tag="ON396_000777"
     CDS             complement(22317..22880)
                     /locus_tag="ON396_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCR3112904.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22886..23749)
                     /locus_tag="ON396_000778"
     CDS             complement(22886..23749)
                     /locus_tag="ON396_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3112905.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23763..24659)
                     /locus_tag="ON396_000779"
     CDS             complement(23763..24659)
                     /locus_tag="ON396_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3112906.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24834..26123)
                     /locus_tag="ON396_000780"
     CDS             complement(24834..26123)
                     /locus_tag="ON396_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3112907.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26095..27612)
                     /locus_tag="ON396_000781"
     CDS             complement(26095..27612)
                     /locus_tag="ON396_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCR3112908.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT         8091 a         5844 c         4791 g         8990 t
ORIGIN      
        1 tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagga
       61 agaggttgta aaatcagcgg tttgacctga gtattagaga tacaaaacgg aaaaatagct
      121 tctcatattt tgccgttttt tgagctaata ccgaaggaca gctgattgaa caccgtttat
      181 tcggaaaaag ggactgtgac acgacttttg tcacagcccc taaaaaaaat aataaatttg
      241 tactattcta tgaagcttct tcttcagaaa actgactgtt ataaagatct gcatagaatc
      301 cgtctttttc catcaatgta tcgtggtttc ctgtttctac gatcgaacca tgattcatca
      361 caataatgtt gtctgcatcg cgtattgtag acaagcggtg agcaacaacg aaacttgtcc
      421 ggttttccaa taggcggttc atcgcttttt gaatcagtat ttctgttcgt gtatctacac
      481 tagatgttgc ttcatcaagg atcaggacat ctggatttgc taagaatgcc cgcgcaattg
      541 tgattagctg acgctgccct tgcgaaatat tgcttgcatc ctcgtttaat acagtgttat
      601 atccttctgg caatttacga acaaaatcgt ctacgtgagc agctttagca gcctcaatga
      661 tttgttcgtc tgttgcatcg tcgttcccat atttgatatt gtcatagata cttcctgtaa
      721 acagccaagt atcttgcagt accattgaaa actgtgcgcg caattcatca cgtgaaagat
      781 cacgggtatc tacaccatcg taacgaatac tacctccaga aacatcgtag aaccgttcaa
      841 gaaggttgat cagtgtcgtc ttccctgcac cagtcggacc tacaatagcg accatctcac
      901 caggtttgac atccaagtta aaatcagtca ttagcaagtt atctggtgtg tagccgaatt
      961 gaacattttc aaaacgaact ttgtatggtg tatctttttc ttctggaagg ccagattttt
     1021 cattggacat ctcttcttca tccaacactt caaatacccg ttcggctgaa gcaactgttg
     1081 attggatagt gttcatcaaa cttgcaagtt gagtgattgg ttgagagaac tgtgttgtat
     1141 attgcaagaa cgcttgaaca tcccctaatg tcatgttccc atttgcaact tttactccgc
     1201 ctagtacagc gacgaacaca tagcctagat ttttaacgaa attcatcaac ggcatgatga
     1261 ttgccgaaat aaattgagct ttccaaccag cggcatacag attctgattt tctttttcaa
     1321 atacttcctg gtctttttca gtatgattga atgttttaac aatggtatga ccaccatatg
     1381 tttcttctac ttggttattc aataaaccta aacttttttg ctgatctgcg aaaaacactt
     1441 gtgatttcgg cgctacgatc atcacgatga tcaagcttaa agggatcatc aataacgcga
     1501 ctaacgtcaa ttgccagctg attgaaagca tcatcacgat gaccccaatc aatgtgactg
     1561 cactagttac aaattgagtc aaattttgct gcagcgtgct tgcgatgtta tccatatcat
     1621 tgattgctcg agacatgata tccccatttg tatgtgtatc ataataggaa atcggtacac
     1681 gattcatttt ggcttccaaa tcatgacgta attcataaac tgttcgttgg gaaacacgtg
     1741 tcataataaa ttgctgtaaa aagttgaata cagcagaaat caaatacatc acgattactg
     1801 tcgcaatgat ctgcgcgatt ttatcaaagt ctatcggaaa ttttgttact tgttggcctt
     1861 gttgcatctg ttgagcgcca gccattacac ctttgaatat ttctgtggtt gctttcccca
     1921 agacctttgg tgtttggatt tggaagatca cggctgctat tgctagtaca aatacagcga
     1981 tgatcgcaac catacgttta gacatatagc caaacaatcg gcgtaccgtt ccccagaaat
     2041 ttttaggctt ttcacctttt acaccgagat ttctgccagg tccaccatgt cctggaccgc
     2101 ctgctggtcc atgagaggga cgtgtgtttg ttttactcat gctaagtcct cctctcttag
     2161 ttgtgaatga acgatttctt gataagtttc gtttgttgct aatagttctt catgtgttcc
     2221 tttgccaacc agttttcctt catctagcac aaggatcata tctgcatcca ttactgtact
     2281 tactcgttga gcaactaaaa cggtgatact ttctttcata ttcgttttca atgcttggcg
     2341 aagattggca tccgttttga aatccaacgc tgagaaggaa tcatcaaaca catagatatc
     2401 tgctgttttg actaatgccc gtgcaattgc cagacgctgt ctttgtccac cagagaagtt
     2461 tcctcctcct tgttccacat ggctatctaa gccatcttcc atctctgaaa caaaatcttt
     2521 agcttgtgcg atttctaatg ctttccaaat catttcatct gttgcatccg gcttaccata
     2581 ctgcatattg tctcggatcg ttccagagaa cagaactgct ttttgaggaa caaagcccat
     2641 taattctcgc aaattatatt gagtcatgtc actaatgtcc gttccattta tttggatcga
     2701 accagattcg atgtcatata atcgagggat caaattgact aaggtcgttt tacctgaacc
     2761 agtcccccct atgattgcga cgatctcgcc cgacttggct tggaaatcaa tatcttccaa
     2821 tgcaagtttt tctgctccat gatatcgata attgacatga ttgaaggcca atgttgcgtt
     2881 ttttccagca aaagaaactg ttttaggatt ttcagggtca tggataccga ttttttcatc
     2941 caacacttca ttaatacggt cagctgaagc ttgtgcacga ggcaccatga taaaaatagc
     3001 ggaaagcatc ataaagctga tcaagatctg catcgcataa gtcataaatg caatcagatt
     3061 accgacttcc aaagtcatat ctgctatgta gtgtccacca aaccaagtga ttgctacgtt
     3121 tgtaccgctc atgatcaatg tcatcattgg catcatcaat gcgacgatcg tattaacttt
     3181 gattgccgta tttgtataat ctttattcgc taaatcaaaa cgattttctt caaagcgtgt
     3241 cttatcgaat gctcgaatca ctctgacccc tgtcaatcct tctcgaaata ccaggtttaa
     3301 acggtcagtt ttcttttgca tacttttgaa taatggaaca gcaaagtaca tgattccgcc
     3361 gatcaaaata atcattactg ggatgacaac taagaagatt tttgtcaact gatgatcttt
     3421 ttgataagca agtatgctcg cgccaatcaa cgtgattggt gcattgatca tcatacgtaa
     3481 aaacatttgt gtaaccattt ggatttgagt cacatcattg gtcgttcttg tgatcaaaga
     3541 ggccgttcca tatttatcaa acgcatcttt tgtcagactc tctgattttt tatagacatc
     3601 cgtacgcaat tgcttaccca gtttctgtga ttcacgagta gcaaaaaatg tatttgcgat
     3661 agcagctaag atactgatca aagaaaagcc gatcatcaca aaacctgtat gccaaatata
     3721 atcgacatct ccttgcgcta cccctttatc aataatattg gaggtaagtg tcggcaaata
     3781 taaatctgcc agtacttgga tcaccatgaa aactgcagct gcaattgctg aagtcaagga
     3841 gatccgtttt acgattttta gcatctagtt cccttctttc tatttatata ctccgttttt
     3901 cagccaagaa agtttcaaat tgaattcact gattgcttgc tctactgagt aatcttcaga
     3961 tgttgaaaat aagcgatacg aatgcgcaat tgttgtaaat acagtattca tcactaattg
     4021 gatcagtaat tccaattcat gatcattttt tactttcagc ttcgtccgat cgatcaattc
     4081 aagcaactct tttgcatctt gtttgtgttc tttatgtgga tcgtgaggcg ttttttggcg
     4141 actcgtatga ggagcgactt ttcttgaggc acggtaatcc atgttcataa acaagttacg
     4201 ataaaaagaa gcattctcgc cttccaaaat ctcataaatc attttagaaa aataggattc
     4261 gaatcctttg aatagatcac catcggattc tttcattagt tgtaacatat ctttagagct
     4321 gtcacgtctc attgtctcaa agtaataaaa ataaagatcc tctttatctt caaaatactg
     4381 atagaaactg cctctaggga tatccgctaa tttgacgata ttagcaatcg aagcttcttt
     4441 taagggtact ctagaaaact cgattctcgc agcctcgatt agtctttttt gtttttcttt
     4501 aggcaaatgg aaaaatgtct gtgtcggcat atggatcctc ctttctttca agatgacaca
     4561 ttgtcatatg acgctgtgtc attataacac ctcctacctg ttttgcaaga catttgcata
     4621 gtttttttca ttatcatccg atacattaaa aaagtgataa gaggaatcct tccatttgaa
     4681 ttaatgagag ttgcccatta aaatagccac atactaaaca aaggatgaga taaatggccc
     4741 aatcaattag tgtccaagac ttcaataaat tgcctctcga tgctgacagc atcgttttag
     4801 atacaagaaa taccttagat tacacaaaaa accacttaag ccactcgctt tctatccccg
     4861 ctgctatctt gccaagacaa ttaaaaaatc tagatccaaa gaaactttat tatattctaa
     4921 gctatacagg caggcgatca gaagcattag ccgatcaact tactgctaat ggttttcgtg
     4981 ctgtttatat cattggtggg atggaagctc ttcagtcgag tgttgcttga atattgcttg
     5041 ttagattcaa aagaggctgt gacaaaaaat cgtgtcacag cctcttttat accctgtctt
     5101 tttttagtta tttattcttc aaccttatca atatgacttg atagcctttt ctttttctca
     5161 tagttataac aatcatagca ataaaaggag aaggtaagca tatggcatct gttttagtag
     5221 tcaaaggtca tcccctaact gcagaagaat cgcgcacagt taaggcattg acgtcttttt
     5281 taacaagtta taaagaaaat catccagatg atgaagtgac tgttttggaa ctttatcgcg
     5341 atgatattcc ggaaatcgat gaagaattat tatcaggttg ggaagcatta cgtgctggtg
     5401 cagaattcac ttccttatca gaaagtcaac aacaaaaaat tgctcgtttc aatgaattga
     5461 ctgatcagtt ccttgctgct gataaaatcg ttatcgcaaa cgccttatgg aatctaaata
     5521 tcccaacaaa attaaaagct tggttcgata ccgtcaatgt agctggaaaa acgttccgct
     5581 atacagaaaa cgggccagaa ggattagtga caggtaaaaa agcactgcac atccaatcta
     5641 atggcggtgt atacaacgga caagactttg cctctcaata tgtaaaaggt attttaaact
     5701 ttgtaggcat cgatcaagta gatcagctat tcatcgaagg aatcgattat gaccctgacc
     5761 gtgcagatga gttgatgcaa aatgcattag ataaagctgc tgctttaggt aaatcatttt
     5821 aaaaattgag ataaatgcat acaaaagaga cagaggaaaa aatcttcaaa gcagcatttg
     5881 taccttggat attttcccat ctgtctcttt ttgttattct ttctgctttt tcaacggatt
     5941 ttcttcataa agagaagaga ttgttgtgta ccattcaaaa acatgagtaa tcactacttt
     6001 agctgctgca taaacaggaa tccccaagat cacaccgatg attccgaaca atttcccaga
     6061 agtcagcaga actaataaaa tcgttactgg atggattgct aactgattcc ctagtactaa
     6121 tggagagatc aagcgtcctt ctattgtctg ctctactgca aaaacgatca gtactttgat
     6181 gatcatgaat ggacctgcta caatggctaa aaagatagcc ggaatcatcg ctaaaaatga
     6241 tcctaagtaa gggatcaggt tcaaaaaacc cgcaataatc cccaacgtga cagcatactc
     6301 taaaccaatg accgcaaaac caatcataaa catgattgcc acagcaaaag ccacagttag
     6361 ttgtcctctt atataagaag aaacttgact attcatttca ctcagcacat tcaacgtcgg
     6421 tttttgcatg cgtgtcggta aaaatttgac aaaataaggt gctaattgtt taccgtcttt
     6481 caatagataa aataagataa acggcatcgt cagcagagcc actaaaatag tcgctaccgc
     6541 gccgacaaaa cttccaagac tctgaactgt actcttagat atatcttgga tcatatcgcc
     6601 tgccgaattc atgaacttat cacctgctgt ttccaactgt tcgcgaaatt ggttgaacag
     6661 cgggtcccgc aaaatctcgg ttaatttatt atcaatcgta tctacatatt gagggaagtt
     6721 actgatgaag ctcactgttt gttcctgaat cttagggatg atcactacgc tcccccatgc
     6781 gattagtgca acaaccaaaa caaacagccc aatgatactg taaagccttg gtacttttcg
     6841 cttttcaaag taatcaacta ccggattcat caagtaatac aagattcctg ctaaaatgat
     6901 aggcagacct acgattgcta aaaattgcca gattggtgca aacaaatatg aaacttttgt
     6961 aaataaaaag acgatcagca atattaacaa tacaacaagt aaggctgtga ctacttgatt
     7021 atttaaaaac cagcgccaaa accacgatac cggtctgttc ttcttatttt ttccttccat
     7081 aattcctcct gttagttata aaaaatttgg cttcccaaac caatagcagg cagtctatgc
     7141 ggatgaagat agattccatt tgcaatttgt ccttcttctg gacaagcatc aaaaatcaat
     7201 gatacgtgag ctactgtctc taaattccca ttggccatcg gaccgacata atcgatcgta
     7261 tatacctgat catcaaaagc aattgttccg cctgctttca aagaaaaagg ttcgtctttt
     7321 gatacttctt gaatcaccga atattttcgt aaggcagctg ttgctgtttt atcgaacaaa
     7381 atcagcatgg gttcttcctt gtttattgct tctgtcccaa tttctgtaat ctttgcctga
     7441 atcattgcac tctctccttt aattgctttc aaaaacagta taacacattc tcattcttta
     7501 gccgtgtgga ataattatgt gatagaataa aaacgaaaac tatttcatta caattaggag
     7561 gaatcagctc atatgatcga aaagataatt ttaggaacct atacgcgtcg cgaaagtaaa
     7621 ggaatctata cgatcgacct tgatacagaa aaagaagaat tagccaacct ttcattagct
     7681 gttgaagaaa atagccctac ctatgtagca aaaagtaaag ctggcaatct ttacacagtt
     7741 actagtgtgg atggattagg tggtgccggt gcgtacgata aggattttca ttttttgaat
     7801 gcggtaacag agtctggcgc tcctttgtgt tatgtagcgg ttgatgaagc acgtcaatta
     7861 ctatacgggg cgaactatca taaaggtgaa gtaaatgtct accatatatt aggagatggc
     7921 gaactgaaag cagcagattc aatattccat caagaagcga caggtcctca taaaaaccaa
     7981 gatcatgcgc atgtccacta tacggatctg actcctgacc aacgattagt cgtatgtgat
     8041 cttggtacag atcgtgtgta tacttatgat gtgtccgaaa acggcaaatt gaatcttgta
     8101 actaccttca ctgcagagcc aggaacaggc cctcgccatc ttgtctttca tccaaatgga
     8161 caattcgctt atttatttgg tgaattagac agcacagtcc gcgtgctttc ttataataaa
     8221 gaaacaggtg cttttgaaga attacagaaa atatcgacct tgccagaaga attcgacggt
     8281 gaaaatggag gggcagctat ccgcatttca aatgatggca agttccttta tgcttctaac
     8341 cgaggacata actcgattgc tgtattcgca gtttttgaag atggttccca cattcaaaac
     8401 aagcaaatca tctcaacaga aggggatttt ccccgcgatt tcgcactaga tccaagcaat
     8461 aattttgtcg tgtgtgccaa tcagaacacc gataatctga cattatatcg ccgtcatcct
     8521 gaaacaggat tactggaatt actgaaaaaa gatgtctatg taccagaatg tgtctgtgtt
     8581 tattttgatc gttagtaaga aaaggagaat gaaatgaatc agcaagacgt attagacaga
     8641 ctacgggaag aactaaatat tcctttcttt gatgggaaaa tcgaagataa agagtatagc
     8701 gaagaggaat atcagaaact caaagccgat ttacagaact actttgaaca atatgtacag
     8761 aatgtagaaa actaatcagc tttccatact atctgaaaag aacatcagga caagactttt
     8821 ccgtctatcc tgatgttctt ttctacaaat aaaaaaccgc cgagatggtt agactcgacg
     8881 gaaaaggagt atttactcga ttttgagtaa gtacaaggtt gttgttggtt ataggtatgt
     8941 cattatcata aaggattttg atcactctat cataaaggta atattacatt tatattagat
     9001 aactctagtt tttattgtta tcaaagattt cttttatttt ttcaacaaca aattagtcat
     9061 tttttggatg gcagctgtca attggtctgt cgcttggtct tgttttgctt cagatgtatt
     9121 gaatagatct tcatctaccc ctgattcaag atcgatactt tggatcattt cttcacagcc
     9181 tgctacatat gaaagataag ctttttcaaa atttttatga atgcccattg cttttgctgg
     9241 tggacgcaaa gtactgactt tcttaagcat tgcccgatat ttttttgttc cttcttcaaa
     9301 taacaggatg atctcttcaa gagtcgattt gtccagttca cttgtttttc cttcatcaat
     9361 tgcgtttctg atcacttcat atttcggatg cattccttct ccgattgctt ctgtttcctt
     9421 gacgatttga tcgatggact gcacataata gccaatattc ggtaacatgt tcgttttcct
     9481 actttctcca ttgttgttat tatttattct acttgttttt atcctcaaat gacaagtact
     9541 tgaaaaaatt taatggtttc tatacagctc gccattctta ttcgtgctca aagaaaccat
     9601 ctgctaaact atattgtttc cctgcgacaa tgtcatattg gatcaattga tattcgtcgt
     9661 aaatctcttg ttgcgagcga ttgaattgcg cttctttata ccactgtccg ttttgatagt
     9721 acagttctcg ttcaaacgcc ggaagttcct gttctaatga taaaagcagc tgataaaagc
     9781 catttgttgt ctgattcgtc tgttccatta gattcgctgc aaaataaaac ggactggtga
     9841 ttgcatcgtg agtcgtttgc ttttctaatt ctcctttact gtcgaacata agaaattgtg
     9901 tttcatgcaa tgtttgtttg tcattttttg cttggatcgt atctgaatag atccctggaa
     9961 gatgatcccc ccaaaaaaca actaaagtcc ttctagaaag attcttcaat tcttccgtga
    10021 actgcttcag tgccgtgctg ctataggaaa tatcttgcag ataattctct aagtcaagtg
    10081 tcccacttcc atctgaagtt tttgcaggat agtctagatt atcatacttt ccatcgtagg
    10141 gcatatgtgt ctgcatcgtg actaaatgaa tgaactgagg tgttttttct tccttgagaa
    10201 gtgtcattac ttctttgtaa gctgactcat cagaaatata tggattattc tctatcgtat
    10261 ccgtataggt catcgtacgc tcgctgatga actggtcaaa tcctaatgtc tgataaacat
    10321 cctctctttt atacatcgaa gtattgtatg gatggatcgc tgtcgtctgg tatgattgtg
    10381 cgtttaacgc tgaaacgata gaaggaagct gatccatttt tggtacaagc atcgtgtaag
    10441 gggtggtgag ttgcgcatta aataacgcca tagaaaaacc agtcaatgct tcaaattcga
    10501 tatttgctgt tccaccgcca tagttttgag aaagcatatt gccactatat gtttgatcag
    10561 ctacttcata ataatctgcc aatggctcac cagatacttc tacgccgttt aatcgactag
    10621 gatctgagaa actttcactc atcacgaaca cgatgttcgg cgactcttcc tctacagttt
    10681 tatttttttc atcggctagc ttctggtatt tttcagtgat ttctttgatc ttttctttcg
    10741 aatatccttc aggttcatcc attggttcta ccttcagatt gtacaaaaac ccaccgataa
    10801 aaccagtgtt atagtagttc attttctggc tgtaaggtat ccatagtgcc gttttattat
    10861 atgcttttcg caacaaatta tcgggattgt tgaaattact gatataccct agcagtccaa
    10921 ttgccacaag cagtgtcgtt aatcggatca attggaatcg ccttcctttt ttcaggcttc
    10981 gaaacatgta ccagcaaaac aatcctatca cggttcctgc ggcagctaag atcactgtaa
    11041 acagcatagt gccggtcata tctttcagca atccgatttc agtgatcatc ttcaaatcgt
    11101 ctggataaat cggttcttgc cgataactca ttttcatata atttgcaaaa cctaatacac
    11161 cgatggctac gacatacgta agggaaccta aagggacaga tcctgctagt gcaatcagaa
    11221 aaataagaat aatcagcaag acgagacagg ctaagaagaa tttttccgta tgccaagaaa
    11281 aagcaaattt caacgcaagg tctacggata gatcattttg acaccactgc aaaaataaat
    11341 tactaaaaac aacaagaaaa aagatactga tgattgctgc tgttcctttt aaaatctttt
    11401 tctgtttggt cgaaagtctg cttatatatc ttttcaaatg cttgctcctt catttctatc
    11461 aataaaagaa tgttgcttct cttattttag ttgtacgaaa ttcgaaagta aagaacggaa
    11521 agaagaatta atgaaatcca aatattctct ttctttatgg cctttctggt ttctttaata
    11581 agcggacctt tcgtactcca gaaatttttt ctagctgttg tttcacttga ttgagctgtt
    11641 caaaatcagt actgtctatg tcaatcaata tgtagacatg ctcattctta tggttacgcg
    11701 atatatctgc gatcgctagc tcgttctcat tcagcagaga aaatatctct gctaatatat
    11761 tggttttctt ttgataaaat atagtgaatc gaaacggcgc ttggaaaaac attcgtgcgg
    11821 atggataatt gaccgcttct ctaaccgtac cgaacaaaag ataatctttt aaggcttcaa
    11881 ctgccaaacg tccgctgtct gccaaagctt cctgagtcga tccccctaga tgaggcaaca
    11941 taagtatacg gtcattttct aagaactctt cttctggaaa atcagtcaag tatttagcca
    12001 gatgttgttt ttccaatgct tgcataacag catatttatc aacaatttca cttcttccta
    12061 cattgataag tacagcatct tttttcattt tttctatatt cgcctgatcg atcaatcctt
    12121 ttgtatcttc tgtcaacggc agcatgacta cgatgtaatc cgaggtagaa agaactgttt
    12181 ctaaatctgc ttgccgaaca taatccaact gggcatcgcg gatagaataa cccaagacat
    12241 ccatccctaa gctatagcaa cttagcgcta cttttgtgcc tatcgctcca agaccaagaa
    12301 gaccgatggt tttgccttgc aattctcgtc ccacataagc acttcttttg ttttctgcct
    12361 gttctaaaat attaggacct gttagtgttt gtaccatacg acttgcttcg attatcggac
    12421 gggaagataa gagcaaacag cacaaaacaa gctcttttac tgcattagca tttacacctg
    12481 gtgtattcat gacgatcgtc ccgttttcag aagctttttc aacgttgatc gtgttcaccc
    12541 caacaccagc tcttgaaatt gctagcaaat ctggtgtaag ccattcatct ttcactttcg
    12601 tactacgaat aactattcct tcggctggca aatcatctat tattttgaaa tattcccctt
    12661 gtaacttttc tatctcttgt tctggaatag catcaatcaa atgaacgtga tacataaaac
    12721 cccctcctta ctaacaagta tctcgaaatt tctgcaatta gacaactgtt taccatttga
    12781 cttcctagaa gcaaaaagag cagttagagc agagtaggct gagtgataaa aataaggcta
    12841 tgacaagtta ctgccataag ccttattttc gagagcaaaa tattgagggt gagatatctt
    12901 gataaatggt gttccgttag ctgacattga taataagact aaatttcaaa aaagttgaaa
    12961 agcaaatttc gtcaatctct tcttatttct taaaactaag tggctttttc acaacctctt
    13021 tttatctaat aaccactgtt tccaactcaa ctaattttgc ccacgtttca atttgatctg
    13081 ttgttagatt tagcgagaca acggtatgat gaccgccacc tgcctgaatc catgaatgga
    13141 caccttcatg gaaattcggt ttagttttcc ataacacacg tgcaacaggt aaattaggtg
    13201 cttcagtcgt tggttcaaag gcttctactt cattgatcag cagtttataa tgcgtaccga
    13261 aatctgccat cgatacgact accccttctc ctgctttccc atcaaataca agacgcgccg
    13321 gatcttctct atctcccatt gataaaggag aaacaacgat tcttggtttc gtcgcagcaa
    13381 gcgtcggatc tacttccatc atatgggatt ccaagattgc ttcttggcca ctagctagct
    13441 cgtaagtata atcttccatg aatcctgtat ctttccctcg agccatgatt ttcagcaacc
    13501 gatcaatcgc tgcagttttc cagtcgcctt ccccagcaaa accgtatcct tctgccatca
    13561 aacgttgtac agctagtcca ggtagttgtt tcattccata caggtcttca aaattagtcg
    13621 taaaagcgtt atagcctcct tcttctaaga aacggcgaat accgatttcc tgctgagctt
    13681 gtacttttac atgttcctcc catgtctttt cttcataatc accatagtcg aaggtataca
    13741 attccttgta ctcttcaaat aattgatcga cttcttcgtc tgatacgcga tcgattactt
    13801 gtacaagatc tccgatacca aaataatcaa cagtccaacc aaattgaatc tgtgcttcca
    13861 ctttgtctcc ttctgtgaca cccacgtttc tcatgttgtc accaaaacga gccactttta
    13921 ttccaaaact ttcattatag gctactgcaa catccatcca atcagcaatc tctttttgga
    13981 tctcaggtcg tttccagtag ccaacgacga ttttattttg tttatttaat cgagcattga
    14041 tgaacccata ttcgcggtca ccatgagcgg attgatttaa attcatgaaa tccatatcga
    14101 tcgttttcca aggaatgctt tcgttatatt gcgtagctaa atgaagcaat ggtttttgta
    14161 gaagctttgt cccgcggatc cacatttttg ctggtgagaa agtatgcatc caagtgatca
    14221 cacctgctac ttcttctcga taattgacct ctttcatgat ttttgtgatc gtatcagcag
    14281 taactgctaa ttcttgaagg cggattggat aaggcaattg gccattttca ttcagttcat
    14341 cgaccatttc ttgtgcatgt ttctttactt ctcgcagtgc ttcttcccca tataaatgct
    14401 gtgatcctac aacaaaccaa aattcttttt ctccaatatt caacataatt agccactcct
    14461 tttatttgtt ttgtccgtaa tacgcatttt ttccgtgttt tcgtaaataa tgtttatcta
    14521 aaatccgttg aggcagctct tctgaaaatg aattgagctg tctggtaaat aaattcatct
    14581 tgcatacttc atctagtacg actgcgttca ttaccgcact ttgcgcatct ttcccccaag
    14641 taaatggacc gtgtccgtgt aaaagtaccc ctggaattgc taagggatca attttgcgtt
    14701 ctttgaatgt ttctatgata acttttcctg tttcatattc gtacccgctg tcgatttcct
    14761 gttgtgtcaa aaatcgagca caaggtaccg atccataaaa agtatctgca tgcgtcgtcc
    14821 ccatcgcagg aacatccaat cctgcttgtg cccagatcgt cgcccatgtc gagtgagtat
    14881 ggacgattcc tccgatttga ggaaatgaac gatataaaac agcatgagta ggcgtatccg
    14941 aagagggatt gagtttacct tcaatgatat gattgtccaa gtccacaacg accatgtcgt
    15001 ctgctgtcat ctcttcataa ctgactccgc taggtttgat aacgaaatat cgtgtttcag
    15061 gatcaaaagc gctcacattt ccccatgtat atttgacgag tccgtgttta ggcaagtcaa
    15121 gatttgcttg gtatacttct tctttcagtt gttccagcat tttctatcac tcctttttct
    15181 tgcaattgat gaatcgctgt tttttcaatg tcgatcccct cacggtaacg tctcataaat
    15241 atctgatagc ctgctatttc ttctgctgaa ggtgtgatgg tgattccttg gtcttgtcca
    15301 aacacgtctt ctgccaagaa gttttctaaa gacttcccat gtcgatccat catataggct
    15361 gctaataacg cgattcccca cgctccacct tctccggctg tgtccatcac tgtaactggt
    15421 gcctccatag cagatgctag aatccgctgt ccaacttctg gtgttttaaa aatcccccca
    15481 tgccccacta aacgatcaat ttggacattc tcagatttca atatatccat tccgattctc
    15541 attgcaccaa aagcactggc taaatggatc cgcatgaaat tcgctaaatc aaaacggcta
    15601 cctggtgtac gtacaaataa tggccttcct tcatttactc ctgtaatgtt ttcaccggaa
    15661 aaataaccat aagaaagtaa tcccccgcaa tccgtatcgc cttctagcgc tttgttgaat
    15721 aataccgtga ataatttatc tctgcttatc tccataccca aagaattggt aaattcttca
    15781 aaaatactga cccaagcatt gatatccgaa gagcagttat tcgtatgaac catgcctact
    15841 agttctcctg aaggtgtagt gaccatgtcg atttccggat ggacattttc tagttctttt
    15901 tctaatacga tcatagcgaa ggcggaagta ccagccgaaa tgttccctgt ccgtttggct
    15961 acactgttcg tagcgaccat accggttcct gcatcccctt ctggcggaca aaccgggatt
    16021 cctggtctca aatttcccgt agggtcgatc agataagccc ctgattcgct taacttgcca
    16081 gcttcttctc ctgatactag aactttaggc aaaatgtgat ccaagtcaat cgctacgtgc
    16141 gctttttcga ttaactgacc aaaaatattt ttcatttctt gatggtaggt tttcgttgaa
    16201 ggatcgatag gaaacatgcc tgatgcatct cctacgccta gtactttctc accagtaagc
    16261 tgccaatgga cataacctgc aagcgttgtc aaaaaattaa tttctttcac atgttcttct
    16321 ttattcaaaa tagattgata caaatgagcg atactccatc tctgcggaat attgtattga
    16381 aatttttccg ttaatattcg ttctgcttct tcagtgattg tatttcgcca agtacgaaat
    16441 ggtactagta actgttcctt gctatcaaat gctagataac cgtgcatcat tgcactgaac
    16501 ccaatcgacc cgattgtcgt aagattctcg ccatagattt cgaatacttc tgctgccatt
    16561 tttcggtaac tgacgcgcag tcctttccaa atatcttcta atgaataggt ccagatacca
    16621 ttttctagtt tgttttccca ctcataacta ccagcggcaa taggagtaaa agatgaatca
    16681 atcaatacag ctttgatcct agttgatcca aactcaatcc ctaaagatgt acgaccatct
    16741 atgacatcta acatactttg cgcatttact tccttcataa tgatttaact ccctccttct
    16801 cgtcaaacct gatcaaaccg tctttttagt agcgcttaca atgattattg tatacttttg
    16861 tacgtacatg tcaagcggtt atttacaaga atacaataaa aaacacttgt tttgtacgta
    16921 cataagttat aaaatgaaat tgcttacaaa atctgatcaa ccgccttctc gaaattctag
    16981 aaggagagaa tacttatgaa aaaagaaaaa aaagtttcct catcgtttat ttattttttt
    17041 ggcgcatttg gcggcattct atttggatat gatattggtg ttatgaccgg cgccttacct
    17101 tttttacagc atgattgggg tttggcaggt aaagcttctt tgataggatg gatcacgtct
    17161 tcggtcatgt taggtgcgat ccttggcggc tctttatctg gtcttctttc tgataagctt
    17221 ggacgacgaa aaatgattct tctttctgca cttattttta tggctggttc tgtcctttct
    17281 gcatctgccc ctcacaatgg ttcttatttt ctgattgcag caaggattct tctaggttta
    17341 gctgttggcg ctgcatctgc cctagttcct gcttatatgt ctgaaatggc tcctgctaga
    17401 ctaaggggaa gattgtcagg gatcaatcaa gtgatgattg cttctggcat gctcctttcc
    17461 tatgttgcag attatttgct aaaagtgttg cctgaaacga tggcttggcg cgtgatgtta
    17521 gggctcgctg ctgtccccgc gctgatttta tttttcggtg ttttagctct accagagtca
    17581 ccgcgttttc ttatgcaatc tggccgcttg gaagaggcaa aaagagtatt gaactatatt
    17641 cggacaccaa aagaagctga acaggaattc gaacaaatcc agctgaatgt caaacaagaa
    17701 aagacaaccg gaacttcttg gcatactttg tttttagaaa aatatcgttc cttagtcatt
    17761 gccggtattg gtgtagcagt attccagcaa tttcaaggag ctaatgctat tttttactat
    17821 attccgctga tcgtagaaaa agcgactggg catgcagcga gcgatgcatt gatgtggccg
    17881 atcatccaag gaatcatttt agttgctggt tctttgctct ttttggttat tgcagacaag
    17941 ttcaatcggc gaactttatt gaaaataggc ggtagcgtga tgggactttc attcatccta
    18001 ccagctgttt taggaactgt actggatgct catacaaatt cgttgttgat tctcttgttt
    18061 ttatgtattt atgtcgcatt ttattcttgc acatgggctc cactgacatg ggtaatcata
    18121 ggcgaaatat tcccactagc tgtccgcggg cgagcatctg gcctagcctc ttcttttaat
    18181 tggattggtt ctttcctagt tggcttgctt tttcctgtga tgactgcttc tatgtctcaa
    18241 gaaatcgttt tcggcatttt tgggatcatc tgctttttag gggttttatt tattcaagag
    18301 atcgtaccag aaactcgtgg gaaatcttta gaggaaatcg aacaaagtgc atctaaaaaa
    18361 acttatccta aacgtatttc tatccatcat tcttaaagag gttgtgacag aagtggtcag
    18421 cttcaagaaa taagaaggaa ccgcttagtt tccagtcaca aggaacaatt tcaaaaaaca
    18481 gcttctcatg tttgatgaat attgtgactt gtgaccagtt ggtaggagaa caccgtttag
    18541 tggagaggtg gtgaaagaaa cgttcagcct tgagcgatga caaaagctct cacaaacatt
    18601 gcgacgagta accgtaggag caataagagg cggtaaaaac ccgtctctta tttttcgttt
    18661 tctttcactg tttcccgttc aactagtaca ggttcataga gaatatttgg ctgtgcctgt
    18721 cctttttcaa tcgttgtgac gatccaccgt gcggcatctt gtcccaactt ttcttttgga
    18781 tgcgtcaatg ttgtcaattt tttattccct aaatggctta aagaagaatc gtctgttcct
    18841 acgatcgata ggtcatctgg aatatgatag ccattttctg tcaacttatc tagcattaaa
    18901 ctagctattt gatcgttata gcaaatgatg ccatctaaag cttgttcttc tttcaaccgt
    18961 tgtactgttt tttcagcgat tgtttctcgt gtttccgtgg tgaaagtaat gatatcttcc
    19021 gtcaaaaaac gcacattata ctgttcacac gcgctgataa aacctttcat cctgtatttt
    19081 ccttgaagat cgtcgatttt tgtgatgaac aacagacgct gatgtccttt ttttatcaaa
    19141 tattctgctg ccataaaacc gactttcaca tcatctaaac aaatataaga ggcatttaac
    19201 tcttcataag cagcattgat catcaccatc ggaatgcctt gttctctcaa agcaacataa
    19261 agtgctaagt ttgggttata ctgattactt ttcgtaggct caacgatcaa accatctacc
    19321 ccataagcaa tcattttctt caaacactct ttctccaatt cgtgatcgtt attcgtactg
    19381 gctaaaagca atgaataccc agattctcgt aattcatttt cgattcctcg gataatcgac
    19441 ggaaaaatat aatcagagag atatgtggtg atgacaccga tcgtttttgt tctgttcaat
    19501 ggtgtttttt ctttcttata gctgtcatct acatatgtgc ccgacccttt ttctttacgc
    19561 agatagccct catttaccaa cacagcgatt gcttgccgaa tcgtatgtct gctgacgtca
    19621 tatatttctt gcagcttgaa ttctgaggga atggttgtat ttacttcgta ttcctcacga
    19681 aggatctttt gttttatctc ttctgcaata tcttgatatt ttgccatttg ccttgcccct
    19741 ttttatttgt acgtacatat atcataacga gtctgcaagg tttattcaag caaaaatcaa
    19801 ggtttcttgt gctcataagg catacggaac aggttcacca aattctggaa atccttgctc
    19861 attccacgta aagcattgcg cgttcgcgtg ccgattagga ttgtctaacg gatccccttt
    19921 ttggttcttc tctggtcgag catgatagat caaaatatca aagcacccgt cttctgaaac
    19981 cgtaaacgag ttgtgaccag gaccaaattg cttatttttt tcagaagaaa cgaatactgg
    20041 ctccgataat ttatgccatg attcttttaa taaaagattc ttgtgctcat cagcccacag
    20101 caggcccatg gcatagtttt catctgttgc actaccagaa tacgtaatga atattttccc
    20161 attgcgagta aggactgctg gaccttcatt tactgaaaaa ccccgttttt cccattcata
    20221 ctcaggtata gaaaggagga tctgcttccc ctttaacgtc caaggattat ccatttcgga
    20281 tagataaaga ttagaattcc cagtaatttc aggatcttgt tgggcccaca cataatatag
    20341 ctttccttga gaagagaaag ttgttccgtc taaactgaaa gtttcccttt tcgtttttat
    20401 ttgtcccttt tctatccaat ctgtattcat tggatctata tgctgatttt caagtacgaa
    20461 catgcggtga tgatgagatt catcacgtat agttttatga ttgcttgcag caaagtaaat
    20521 ataccatttt ccattgatat aatggacttc aggtgcccag atcaattcac tcatcggtcc
    20581 ttcttcatga gcctgccata caatcaggct tgcagcatgt tctaattcat tcagtgactt
    20641 tgcccttctt aattcaatca cttggtagcc tggaaccgat cctgtaaaat aataataacc
    20701 atctgtatgt ttatataccc aaggatctgc ccgttgccga acgataggat tattataaag
    20761 tgtcttcatc ataacaaatt tcctcacatt ttctttattc taattacatt ccaagagtat
    20821 ttttctatcc ctacatttaa ttcgtcatgt ttttctattg aaaaatagtt tatttcaggt
    20881 tttatttttt ctggttcttc acgtgtattt ttgtccaaca gatcgctact ggttaatgtg
    20941 tagtgctctc cttgatctag caaattgaaa tcatgttcaa tcgttatctt ttgttgttca
    21001 tttgtgcgat tgatcaagaa aatagtcagc tgattgccgt tatctacgat cacactgtcc
    21061 acataaggaa cattttttat cttttcacaa tcataaacag ggcaaatgat ttgttgtttc
    21121 aatacttgac ctttagcata tttcgataag tgttgcatca cataataagt tggattcctc
    21181 catgcttgtc ctttagcatc tgtcagtatt aaaggtattg tgtttactaa caatgattga
    21241 caactaatct ttacgcgatc tgcgtgtcgt aaaatagcca gccctaatga accaaataat
    21301 aatgtatctt ccatagtaaa gtaacaccaa tcgatgggac taccgacttc aaaatcccta
    21361 tgtctttttt ccggttgggc atgtacattc cattcatcaa acgctagata cattgttttc
    21421 tcacttctca aatatccttt tacataatca caagtagcaa cgatttcttc aatttgacga
    21481 tcgaatcgat aagcttttgc aaggtatgtc gctgtatcat cacgctcatt tttaggaggt
    21541 ttagtcaagt cttcttcatc atacttatca atatagttat gcaaagctaa ataatctaca
    21601 ttctcataag cttcttctaa tgcgatacga tcccattcag ggtacgatgg taaacgtggc
    21661 gtagagcttc caactaagat tgtttcaatt gaatcatcca ctaacttcat gacttttgag
    21721 gcttcatttg ctaacctacc atattcataa gcactcttag cccccacttg ccatggaccg
    21781 tctagctcat tccctaaaca ccataattta acgccaaaag gctttttttg cccatttttt
    21841 attcttaaat cactccagaa ggttcctttt ggaaaattac aatattctac taaatttttt
    21901 gcttcatcaa ttccttttgt tcctaaattc accgtgaata tcggtttcgc ttgtacatga
    21961 ctgatccatt taaaaaattc gtggagtcca aaagaatttg gttctatacc tcgccatgct
    22021 aaatcaattt tttttggtcg ttgttctttc ctacctacag tatccaccca atcataacca
    22081 gatgtaaagt ttcctccagg atagcgaatg acacctaatt ctaattcctt tacataagag
    22141 aggacatctt gtctaaaccc gtcaccatca gctgtttcat ggcccggttc ataaatccct
    22201 gtatagacga cacttcccat atgctcaaca aatgagccaa acagccgaga actgatctct
    22261 cctaccgtaa aatcctctct gatttttact ttcacttctt cgtccaacag ctttccctac
    22321 ctttcatttc ctatagtcaa gataatagat acaagtttcc atacgccacc agcaaagata
    22381 aaaataaaag gagggaaaag aaatatgagc agtacaaatc ctaataacaa tacgcaacaa
    22441 agaatatttc tcaaaaaatc aaaaaatact ttccatgcac tttgttcaaa aatttgtaaa
    22501 aatgtgatat ctttgttttc agaaatcaca tatatccagg ataaaagaaa catggaataa
    22561 ataagtaaaa ctacatatcc actaattgaa agtacgattg tctgttcttc taataattga
    22621 ttaacaagaa aataaataat gatcgttaac agactaagga acaagttctt accaaaatgt
    22681 tgctttattc cttgaaagaa aaaataaatg atatttcctg atccctcacc tttccattgc
    22741 gccattacat aaaaaacagc acatgtagca ggcacgatag taataacagg caaacacacg
    22801 attacccaga gaatacctaa taagatgtaa tcagaaacca caatcaactg tccataaaat
    22861 ttagtctcaa ctattttcat cttcctcact ctttcattcc agacatagct gttccttgaa
    22921 caaaatattt ctgtgcaaaa atataaacca ctagtaatgg cagcattgaa ataaccgttc
    22981 cagccattac aggtccataa aaagaaatgt tctgccctgt aaataaagct aaccctgctg
    23041 gtaaagttct catttttgaa ctacttgtca atatcaatgg ataaagcaag tcattccaac
    23101 tattcatcag cgtgaaaatc cctaatgata aaagagtagg tttgctcaat ggcaacatga
    23161 ttttcaaaaa aatattaaac tcacttaatc catcaatgcg tgctgcctct tccaaagaat
    23221 ctggtaaagt aataaaaaag gaacgcatca tgaagatacc aaatgcagtt gtcatacgag
    23281 gaatgatcaa acctgcataa gtatcaagca tgccaagcaa attgacttca ataaatagag
    23341 gaatcatgaa gacttggaat gggatcatca ttgtcagcaa aacgaaatag aacaaaactg
    23401 attttccttt gaaacgcata cgagcaaatg cataccccgc aagagaatca aagaatacag
    23461 aagtgatcac tgtccctcca gcaaaaatca ctgtattttt gatgtaatct aataaaggga
    23521 tcgttgtcca aaccttaata tagttatcta gggtatagct atctgcaaat attctaggcg
    23581 gaaatgagat aatgtctttt tcgtgtttaa aagaagaaat aatcagccac aaaaaaggaa
    23641 acgcaataac caacaatata actagcatac taatcgttcg aatgattttt ttaccactaa
    23701 ctgcttttcg actattcaca gtaactgtaa tcgagctttc tggtgccata caggtttccc
    23761 tcctatttca tttgtttttc tttccgtgac atgtacacat tagtggagat agctatagtt
    23821 gcaacaatga taaacaaaac cgaagacagt gctgatgcat agcccaattc ataaggtgct
    23881 gtaaatcctc gactgtaaat atattgaaca gctgtttctg tcttaaattg tgggccacca
    23941 gcggttgcaa catatacttg gtcaaacact tgcatcgaac caatcagatt cgttaaaata
    24001 caaaaaccta aggagggaac gatctgaggt agcgtaatgt taaaaaactg ctgtcgattt
    24061 gttgcaccat ccatttgagc agattcatac aaagagggag agattccctg aatagcagtc
    24121 aagagaatcg tcatagtaac accaaaattc ttccaaacag tcatgatggc taccgtcggc
    24181 atagcaaggg ttggatcgcg aaaaaattga gggatcggca agccgacttg tgaaaacaag
    24241 taaggaatcg cccctacatt cggatcaagc aacatagaaa aaataatcgc aattgacgtc
    24301 aatgaacaaa taactggaat gtaaaatgtc gaacgacaaa aacgattgaa tctggtattt
    24361 ttagataatg ctactgctgc taataaacca acgataattt gtgttggcgt ttcgagtacg
    24421 gtgaaatata aagaatgaat caacgaatta actgctcgtt catcactaaa tatacgtgtg
    24481 aaattttcaa aacttacaaa atttcttgat gtaaagaaaa tattcaaatc tgtaaaacta
    24541 atagcaaatg ttcctaaaag cggtatgaca gtaaataata ataaaactaa aaaagatgga
    24601 gttaaaaaca aatacgcagc acgatcagga cgattccaat atttggtgaa tccgttcata
    24661 tagtccacct catctttcat tttgataaaa tatacaatct tggtttaaac gaatctgaat
    24721 aagatatccg aacaatcgct gtgatttttg caataacacc tatctgcaaa tactaggtcg
    24781 atcattcaga tatctgtatc ttgccaacag tctatcctgt ataaagttct gtactatttt
    24841 tgcagtaatt gattgatggc atcatctgca tcgttcatga gtgtctcagg atcttctcct
    24901 gctaataatt tttcaatcag tggattgatg atatcgttat tgatcgcaga gatttgggta
    24961 aaacctggta aatatggctc tgcatagtcc atttgtttcg ataattcaga tacaatagga
    25021 tcatttttta cttcttcatc ttttgctact gaatccaagt aggcaggaaa accattttcg
    25081 atactccatt ttttaccaat ttcagttgta ttccaatatt tcaaaaactc atagacagct
    25141 tcttttttat tattatctgt acttgccgga atggcatatc ctacgccatc taaaatagct
    25201 gtttttttgt ctgaatcact cccttgtggt aaggtagtca cattataatc aatctcgttt
    25261 gctttcaatc cgttatttaa ccatggccca ttgatttcaa tggctagttg accagcattc
    25321 attaaattgt ccatttcggc gccagaaatc gattctggac cagtcttgtc tacatggatc
    25381 attttttgga tcgcttttaa cgttttaagg ttctcaggag acgcaaattc agacttagta
    25441 taatcatcgt tcactaattt accgccattt gctaacatcc agttatataa cggtgcagtt
    25501 ccatccttat tgaagacaaa acctgataca tttttactac tatcagttaa ttttggtgcc
    25561 atttctgtca attcttccca agtagtaggt ggttgttcag tatctaatcc agcttgttta
    25621 aacaaatcat tattccaata catatacatg ccttgtacct gcatggggac aaaatactgt
    25681 ttaccttcta cttcacctaa ttcaatagcc gtttctttaa aatcagattt atctactcca
    25741 tcatattccc agaaatcgtc taatggttgt accgctccgt tttctacata ctgtgcaaaa
    25801 tctggataat ttaatgcaat aaagtcaggt gcttttttag aagaaatcgc tgtaggaagt
    25861 ttttcattca aattggccca agtcatgata tccatttcaa ttttgatgtt tttatcattt
    25921 gttttattaa attcattaac gatttcttgc aatatttcac catcagatgc agtaaaccca
    25981 ttccaaaaag tcaaagtcac ttctttatca gaatcagatt ctgctgaact tccgccaccg
    26041 caagctccta aaatacctgt tgcagctacc aacaaagagc caattaacca ttttttacct
    26101 ttcatttttt gttcctcctt catctgataa acgacatctg accacattcc aagatagcgg
    26161 tttcaataaa caattgattt tcccatcttc agcttttaca tttgttgaat gacttggttt
    26221 tacttgttca cttttaggaa gattagttgc ttttgtatca aatccactca tttcagaaaa
    26281 atcaatcact ccctctaatg ataattcaga caagtgaaca gttagattca tttcttcttc
    26341 tccacggtta actgcaaata tgacaagttc tttgtcattt attacggcta tactttctaa
    26401 atacggcact tcttcaaaat cttccgtcgc atagtttgct gaacgaattt gtgggcttaa
    26461 cacctctcct tgtccataca cagcagcttg cataaatgga taatagatcg tctgtctcca
    26521 agcttctccg tttgtttctg tcctgattgg tgcaataacg tttaccaatt gagcaagaca
    26581 agcaatcttt actctatctg catgtttcaa taaagtgatt aatagacagc ctaatagtaa
    26641 tgcatcttca aaattatagt tatcttctag taaaggagga gcaatctgcc aaggcgctgc
    26701 ttgctcatct tgttccgctg aatgatacca aatattccat tcatcaaagg acaggttgat
    26761 ctgcttctta ctatgtttct tcgccttgac tgtatcgcac atcgctgtca cttcagaaat
    26821 gaaccgatcc atgtctaagg aacgtgcaag ataatgagaa agatcatttt tttggttgcc
    26881 ataatattga tgcagagaca aataatctat ttgatcgtac gcttgatcaa gaactgttaa
    26941 ctcccaatca ccaaatgtag gcatttgccg attcgaactt ccgcataaaa ccacctcaat
    27001 tgaatcatct acgagtttca tcgccttagc cgtttcctct gctaaccgtc catattcttc
    27061 cgctgtttta tgtccaatct gccaaggacc gtccatttca ttccctaaac accatgtctt
    27121 aattccaaaa gggctctctt gaccattcga acgccttaaa tcactccaat aagttccttt
    27181 aggaaaatta caatattcga ctaaattacg agctgcatct actccacgag tacccagatt
    27241 gacagccata ttgatttcgg cccccacatg ctgacaccag ttataaaatt catgaatccc
    27301 aacttcattc gtttcaattg tccgccaagc taaatccaac cttctaggtc tatttgctat
    27361 gggcccaata ctgtcttccc aacgaaatcc tgacacaaaa tttccaccag gatatctaat
    27421 catgggaatg ttcaattctt ttaccaagtg aatgacgtct tggcgaaatc cacttttatt
    27481 agctgatgga tgatctggct gataaatccc ttcatatact gctcgtccca tatgttcaat
    27541 aaatgaactg tacaaccgcg gatcaacttt gcctacacgc ttgacactac taatcaacat
    27601 tgtggcttcc ataactgacc tcctttaatt tatacatgaa taatacacct cgttgaaagc
    27661 gttgtcaata atataaatgt taaacttact tattttttat cgattttaat taacca
//
LOCUS       DAJSKS010000020        27682 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJSKS010000020 DAJSKS010000000
VERSION     DAJSKS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.020"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(235..1179)
                     /locus_tag="ON396_000782"
     CDS             complement(235..1179)
                     /locus_tag="ON396_000782"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCR3112909.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1475..2419)
                     /locus_tag="ON396_000783"
     CDS             complement(1475..2419)
                     /locus_tag="ON396_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3112910.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2640..3854
                     /locus_tag="ON396_000784"
     CDS             2640..3854
                     /locus_tag="ON396_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3112911.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3876..5891
                     /locus_tag="ON396_000785"
     CDS             3876..5891
                     /locus_tag="ON396_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HCR3112912.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5974..7347)
                     /locus_tag="ON396_000786"
     CDS             complement(5974..7347)
                     /locus_tag="ON396_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR3112913.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7373..7657)
                     /locus_tag="ON396_000787"
     CDS             complement(7373..7657)
                     /locus_tag="ON396_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3112914.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7659..9674)
                     /locus_tag="ON396_000788"
     CDS             complement(7659..9674)
                     /locus_tag="ON396_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR3112915.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9853..10158)
                     /locus_tag="ON396_000789"
     CDS             complement(9853..10158)
                     /locus_tag="ON396_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HCR3112916.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10247..11278)
                     /locus_tag="ON396_000790"
     CDS             complement(10247..11278)
                     /locus_tag="ON396_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3112917.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11485..12360
                     /locus_tag="ON396_000791"
     CDS             11485..12360
                     /locus_tag="ON396_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3112918.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12512..13354)
                     /locus_tag="ON396_000792"
     CDS             complement(12512..13354)
                     /locus_tag="ON396_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3112919.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13446..15323)
                     /locus_tag="ON396_000793"
     CDS             complement(13446..15323)
                     /locus_tag="ON396_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR3112920.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15320..16741)
                     /locus_tag="ON396_000794"
     CDS             complement(15320..16741)
                     /locus_tag="ON396_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR3112921.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16744..20133)
                     /locus_tag="ON396_000795"
     CDS             complement(16744..20133)
                     /locus_tag="ON396_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR3112922.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20390..21781
                     /locus_tag="ON396_000796"
     CDS             20390..21781
                     /locus_tag="ON396_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3112923.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21854..22501)
                     /locus_tag="ON396_000797"
     CDS             complement(21854..22501)
                     /locus_tag="ON396_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR3112924.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22509..23195)
                     /locus_tag="ON396_000798"
     CDS             complement(22509..23195)
                     /locus_tag="ON396_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3112925.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23201..24274)
                     /locus_tag="ON396_000799"
     CDS             complement(23201..24274)
                     /locus_tag="ON396_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3112926.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24496..25170
                     /locus_tag="ON396_000800"
     CDS             24496..25170
                     /locus_tag="ON396_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3112927.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25175..26515
                     /locus_tag="ON396_000801"
     CDS             25175..26515
                     /locus_tag="ON396_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3112928.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26664..27650)
                     /locus_tag="ON396_000802"
     CDS             complement(26664..27650)
                     /locus_tag="ON396_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HCR3112929.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8062 a         5241 c         5029 g         9350 t
ORIGIN      
        1 tttgccgttg cggaaggatt ttttaattaa ccacagataa acattgataa cgattcgctt
       61 attgtaatca acagattaaa tttcatatgt tcatatgtac ggatacattt tcggaccgat
      121 taattttaac cattctgttt atttgcttac ctattagata tcaataaaaa actgcttaac
      181 agctatttca gctgttaagc agttttagga tttttagttc atattcattg tttcttataa
      241 tacagtgttc aatacttctc ttaaagcact tgttgatttg ctgaacattg ctttttcttc
      301 ttcgttgatt gataattcaa caacttcacg aacaccattt tcgtcaacga ttgatggcac
      361 acctgtaaag atatcttttt cgtcgtattc accagttaaa taagcagata caggcaatac
      421 agcttgttcg ttattcaaga tagcttttac gatacgtgca gtactcatac caataccata
      481 gtaagttgct tttttacgat caatgatctc ataagcagca ttacggactt tatcagcaat
      541 cacatctaat tcatcttggg caatacgatg gtctttttct acaatttcaa acactggttt
      601 accaccgaca gttgtatgtg accatgcagc aacttctgaa tcaccatgtt cacctaagat
      661 atagccgtgg acgctgcgtg ggtcaacttt taatttcaat gcgatttctt tacggaaacg
      721 agttgtatcc aatgttgttc cagtaccgat aacacgtgat gttggcagac cagattcgtt
      781 ccaagcgata tatgtcaaaa tatctactgg gtttgaagca acaacgataa taccgtcgaa
      841 tcctgatccc atgatttctt ttacgatttg gcgcatgatt gacgcatttg ttttaactaa
      901 atctaaacga gattgaccag gtttttggtt gataccagct gtgataacaa cgatatcagc
      961 atctttacat tcttcatagc cacctgacca tacagccaca ttttcatcgc cccaagccat
     1021 accatctaaa aggtccaatg cttccccttc tgctttttct tgattcacat cgatcaatac
     1081 taattcatta gagattcctt ggttgatcat tgcataagcg atacttgttc caacaaaacc
     1141 tgtaccgacg attactactt tacgacttgt ttttttcatg aataaacagc tccttttcat
     1201 aactatttgt tactttcatg aaaagtatag tcttgtttat tcattttatc aacatgttaa
     1261 ttataatagt tgttattata atttttgatg gaaacgaatt cctgaatacg cataacagat
     1321 aaatgacaaa tgtttacacc tatatcccct gataatacaa ccaaaagttt agaactgaaa
     1381 ataaatccaa aaaaattcgc tgcaacgata taataagaca gcaatcgcac ctaaaacaat
     1441 tttccttcat atgctttagt gaaagatttt cttcttaatg tttatttctc cattcattgg
     1501 gagtacatgt tgaatacttc ttgaataatt tgaataaaga agtcgtttgt ttatatccga
     1561 tacgattagc gatttcttcc aacgaaaaac tcgtttcttg aatcagttcc aacgcaaagt
     1621 cgaatttttt ctttagaacc agttcttgaa aggaagcatc aaaatacatt ttcaatttat
     1681 tactaagata gaccggattg tagccaaaat gagctgctgt ttcttttaac gtacaagaag
     1741 taaaatgctt atcgatataa tctaatactc taagcatctc attttctgcg catccatctg
     1801 ctgatgaaaa gctttgttgt tctaataaat gagtcaattc gatcagtaat agtgatgtat
     1861 agagatttaa cgaacggatc ttatgactgt atgtatcaaa ataaatcaac agcatatttt
     1921 caatcaaatg cgtcaaacga cttgtttttc cgccgcgaaa atgaatgaat tgtgtatgtt
     1981 cccctttttc ttttgatgca tttatcaaaa aagaagaaag tagattattt gtgcttgcca
     2041 aatgattcaa tatctctgta gataaggttt cttcttttaa taagatgttg attaaaatat
     2101 cctcttgccc tgtgtattcg attctttgag tagcttcttt atcaatgatc accacgtcac
     2161 tttcttccat ctctatcctt tctccattaa taatttgtaa acatgtaccg ctatacatat
     2221 aattgatttc aacaaaatca tgtgtatgct cgggaacttc cgcatatcga cggtgtttac
     2281 tgatatgaat atttccctgt tgaaatagat cccctcggtg gatttttggc ggtgtctgaa
     2341 gatggtaagc taagtttaaa tcattgatat tcattccttg ttcacgctgc agagtttcga
     2401 ttgattcttc gttacgcaaa aaaaatgtct aattcagcta gttccaattg actccctcct
     2461 ttttgtaagc ggtttctttc gcttattagt tcacgctgta ctgctattat atgcaaagct
     2521 atttttggtt tcataaagac tttctatggt tgggacatga acttcctgtg attccgctat
     2581 tgagagaata accacttcat actatatttt tgataagcgc ttacaaaaat gaaaggaaca
     2641 tgcttatgaa taaaaagatg atgaaatttt ctctgctgtc agtctctctt attttgactt
     2701 ctgctggtgc agtcacgtct gcaattccca gtatgtcagc tacttatgcc acgcgttctt
     2761 tatctgctgt tgaattatta acgactgctc cctcttttat ggtcactatt tttgttttga
     2821 ttagtagttt tattgctagg aaaatcggac aaaaacaaac tgtgatcatc ggattagtga
     2881 ttgcatcgat ttgtggtatc ctccccctct tctcacaaag ttacagtatt gtcttagctt
     2941 caagagctgg actaggtatt ggctttggat tgatcaattc tttggcagtc agcatgattg
     3001 ctgcctttta tcacggagat gaacgagcct ctatgattgg ttttcaaagt gcatttcaag
     3061 gatttggtgc agctttaatg acatttgttg caggacagct agtacagttc ggttggcaat
     3121 ataccttttt agtctacgcc atcagcttac ctattcttgt cttgtttgtt ttgtttgttc
     3181 ctacacctga aacaactgaa cttcaatcgc atggcacagc cccaacaaga caagtaatca
     3241 aaacgaatcc taaaatgatt agttatggta tttttctctt gctggtcatc gtggtatata
     3301 atgctgtatc gattaaatta gccacagtcc tgacgactta ttctctagga aacgaaacta
     3361 atgcagcgaa tatcttaaca atcatgcaat tggcaagtat ggctgccgga gtttgttttg
     3421 gctggcttca gacaaaagca catcgctata tgcttacact ttctttacta ctgatgggaa
     3481 tcggctttgt attgattgcc actgtgataa acctgtatgt gattgggttt ggtgcgatcc
     3541 taacaggaat ttctttttcg ttattcattc cttacttatt caaccaagta agtatccaag
     3601 cgcccaagta cgcggagagt ttcaatactt caatcttatt ggttggcgct aatttaggta
     3661 ctttcattgc accatatggt ttgctctttc tctcttttgc ttctcttggg agtaaaacac
     3721 ttgctgtttt cgtcaatggc agtatcttgc tgttgattag cgcgcttatt agccttctag
     3781 tagtacacag gacgaataga aagacagtga taagagtaga tgaagaaata aaagaacaat
     3841 tgacaacaaa ataaatttga tggaggtcgt gtaaaatgac aaatgaaaaa cataaagata
     3901 gatggctttg gtatcctgga gactttgaga tccgccatgg actgctacaa aatttccaac
     3961 gggaagaacg aggttttgac tggccagcgt actggtacat ggatgattgc catcggaacg
     4021 tcaagtttaa gagatattac tttcttgatc aaccttccat gtttaaagtg acgatccaag
     4081 gagttgggta tgtagaaatt aatggacaaa aacatccatg tggaaaatgg ctgacttgtc
     4141 cagctggtaa agcaaaaatt cgtatctttg taggacatac atcagggcta ccagcgatgt
     4201 ttatcgaggg agacgaagta aaatcagata ttggctggac agcaagtaat tttattgaag
     4261 aatatccagc tggctggagt ccgctttatg tcgatatcgc aaaagatcca aatcaaatct
     4321 attatcaaaa agaatgcgtc catccagtca ctgaaacgga gattggcgga ggaatcttat
     4381 tcgattttgg gcgagcagtg aatggctgtg tagaactaat atctctggat gaccatatcg
     4441 aatctatcac tctatgttat ggagagtccg atgtagaagc tatggatatc gattattgct
     4501 actataagca aataggaatg acaagaaaaa tgaaagctcg taaacgcgct tttcgctatt
     4561 tgtttatacc agaggtttct actggaatgg ttcaacttca agtttttcat gaatttttat
     4621 ctctttcgaa acaagctgct ttttcaagcg attcttcttt actgaatcag atctggtcag
     4681 tatcagaaga agccttcaag ttatgtagtg atttgttttt tatcgacggt atcaaacgag
     4741 atcgctggat ttggtctgga gatgcgtatc aatcattcat gatcaatcaa tatctttttt
     4801 ttgacgagga aatcaataaa cgaacgatat tagcattaag aggacaaaat gaaatcaagc
     4861 agcatatgaa tactatcgtt gactattcga tcttgtggtt gattggtatc gaaaaccact
     4921 atatgatgac gaaagactat gaatttttac gcatcgtcta ccctaagatg caatcattaa
     4981 tgagatactt gatggagcaa acgaatgaat tagggtttat ttatgggaga gcaaaagatt
     5041 ggatatttat tgattggtct gaaatggata aagaaggaac gcttgctgca gaacaagtct
     5101 tattactgaa agcctatcat tccatgatca cttgcggaga tatcttagct gaaacatatg
     5161 gagagaacga aaattatcat actcatctct atcaagaaaa atatcaacta ttgaaacaaa
     5221 acctatttga ctacttttgg gatgaagaac ttggcgcttt tatcgattgc tatgaatctg
     5281 ggagaagaaa tgtgacgcgt catgcgaata ttttcgctgt tttattcgat tttgctaaca
     5341 aagaccaaca gcaatcgatc ttaaagaatg tcttattgaa tgacactatc acacagatta
     5401 ctactccata tttcaaattc tatgaacaag atgctttatg caaactcgga cagacgaata
     5461 tcgtctacca aacgattctt gattattggg gagggatgtt atctcatgat gcggtaactt
     5521 tctgggaaga atacgaccct actcaaacag gaaatgaaaa gtacagtatg tatggagatc
     5581 cttatggaaa aagcttatgc catgcatggg gagcaagccc aatctatctt ttaggacgct
     5641 attttatcgg tttgtatcca acaaagccag gttatgaaga atttatgata caacctaatc
     5701 tagattcatt tgaaacactg aattgtacgc tacctatcaa agatggaaag atttcgcttg
     5761 aaaaaacaaa agataagatc gtgattactt ctgatagaac aggcggaaca gtgaaaatga
     5821 atggtaaaag ttatcctctt ctagcaaaac agccacttgt cttaacaagc tattcaaaaa
     5881 taaatgcata aaacaaaaaa ctgcttgcga catatcagaa gactttaaat cttcttctga
     5941 tattgtccaa gcaattttta ttttgaagtt tgcttaattc tgttctttag aaacaatgtt
     6001 tgttcttttt ccggcaaaaa gaaagctaga taatacgaca agtaaaaaga aagcgatgat
     6061 tccaactgtc aaggctattt gaccgtattc tcctagtttc ccaagtacaa gtcctggtac
     6121 tagataatca gtatcggcaa atgtagaaga agcgatttta agatctccta atactggaag
     6181 gataaataat ggcagccaac taatcaataa gctattgaaa gcagaaccta ataccgcccc
     6241 tcttctaccg ccagtcgagt tgccgaagac accagaagca gctccgcaga agaaatgtcc
     6301 tactacccca ggaatgataa ctgtagtttg caagcctatc atagcaaaca tggatagtgt
     6361 tcctacaaag aagctaacaa aaaagccaat taatacagca ttaggtgcat atggaaaaac
     6421 gataggaaca tctaatgctg gtttagaatc gggtacgagt tttttagcaa tcccttggaa
     6481 tgctggaacg atttctgcca acaccattcg aaccccttga aggacgataa caaacccagc
     6541 tgtgaatgtt ccagcttgga tcagtgcaaa gattacagcg ttgtctccat tgcttagttc
     6601 tctttcaaca aatccaggac cagctaacaa agcaagtgcg acatatacga tcgacatcaa
     6661 gagcatgatc gatacagtag aatctcgcaa aaagctgaaa cttttaggaa tctttatgtc
     6721 ctctgtagaa cggtctttgt ttccaaaaaa ctttccgatc catccactag aagcataacc
     6781 gatatttccg gtatgcccta acgcaacttg attgtttcca gtgatgttct tcataaacgg
     6841 ttgacaaaga gcaggcgtaa tagttaatag tgttccttcg aagaccccgc ctaataagat
     6901 catccacaaa tttgcagtaa accccgcgct tactaaaatg actgctgtta gacaagaaac
     6961 ataaagcatt gcttgacctg taagaaaaat gaatttatat cttgtgaatc tagcaagtaa
     7021 aatattcacg atcatcccta ctagcatgat caaagcagta ggtgtaccgt attgttgcaa
     7081 agcaatcgcc actactgctt cattgctagg aactactcct tgagtatgca gagctgtttg
     7141 gaacatcgaa gcaaaagggg ttagtgaatt actgattaaa ccagcaccgc cgatcagtac
     7201 taggaatccg acaaatgttt tgatagttcc ttggatcact tctgaaaatt tcttcttctg
     7261 aagaacgaga ccaagcagtg caatcaaaga aacgaggatg gagggctgac tgacgattga
     7321 cacaaacaaa ttcaacaatt tatccattga tagttcctcc cttgaatcat ttttattctt
     7381 ttgacaagat cttcgttact ttttctttta cttcttcgct gtcaatgatc gattgcaaag
     7441 gtacgagctt ttcatcaggt atagaagtta gcgtatcttt caaagaatgc tcaatgaaga
     7501 aataatcagc catatcgcta gatacacttc caacatccgt atgacttact tcaaactcgt
     7561 ccacatctgc tccaagatct gcaagtgctt gtttgatgtt catctctacc ataaaacttg
     7621 ttcctaatcc actttgacat actgcaaaaa ttttcatatc attcgtctcc ttttcttatg
     7681 atcgctaata tttcttcttt tgttgatgcg tgtaataaat cttgaagatt ttgtttatta
     7741 gaaagtagct ttgttaaatt tgccaaagcc cgaagatgtg cctcattatc gatagcagct
     7801 aaacagatga acaaactaat gggatgggct ggattatcaa gcagatccac aggttgttga
     7861 agtttcagca aagacatccc tacttcgttt acgccatctt ctggacgagc atgaggtaat
     7921 gcgatgtgat cgccaatatg gataaacgca ccatactgct ccacgcgctc gatcatagct
     7981 ttgatataac tttcttctat tttattttct gataatagcg gctgtgcggc aaatgtgatt
     8041 gcttcttgcc agttttttgg ttgatccgat aattggatca tttcttctgt taataattca
     8101 gatagcatag gacgttgatc ctccttcttt tctaaaagtt tgttcatttt acgatttaat
     8161 acttgataga gcttttcttc gtctacgcct tcttttaatt cgatataagg cagtagcgcc
     8221 gacaacaatt cttgggcaga aggcattgaa aatcctggta tcagccaatc tctttgtact
     8281 tgattgatca gctgattttt ttccaagtca gtcatgattg gatgcagaac atagagagac
     8341 tttttcttat cagtaagtgt gagaggtaca gtagaaaaaa taatatcgta agaagtttcg
     8401 tcaacctcgt gtaagcggtc agctgaatta gtctctgtaa aaaagatcat cgggaataat
     8461 tgctgaagtt cactttttaa gatcaatgaa gagctaatgc cacttggaca gacgatcacg
     8521 gctcttattt ttctatttct ttcttctgcg tccgcttttc tgatctctcc gccaaatagt
     8581 atagtgaaaa aaccaatctc ttcttcaggg atgctcttcc ctattcgtct ttctaacgga
     8641 agtaatgctt ttcttgtcat ttcgtagatg gaagcatatt gatgcttaat ttgatcgatc
     8701 atcacattag gtaaataaaa agaataggaa atacgaaaat aggcagggac caagtgataa
     8761 aagacattca tcaataattc acgaaacgaa gcaaattcta cggccatcag gaattccatg
     8821 cgatggatga cttctgaaga acattccagc aaaaaatcaa gtgcaggctc ctggacatct
     8881 ccttcaacga tcgatgctaa gcaaagagag aaaaatataa tctccgaatt ttgatgttcg
     8941 gaatagccaa ggccagtcaa tacttgtcga ctaatgtcag tgactgcaag agaagccaac
     9001 agttcagcat cttttaccag ttccatggat gtttttccaa ttcgaatggt tgtcaaactc
     9061 aaataataag ctaattcatc cattcgattt ggtgcgaatc gaagatctga tagtgaaatc
     9121 gttttatcta gcaaatctct aactttggcg taataatcaa tggaaagggt tgcggttaaa
     9181 tacgtgagcc caaatcttcc agcaagtgac tgcatcaaac gagcagtgcg attttttgct
     9241 tctcttctta atattgcttc tgaccctagc agcctaaaac cagttttcct agtatacgac
     9301 agctgacaat cttccacttc taattcattt cttatctttt tgatatctga caggactgtc
     9361 cctttagaaa cctgaagaaa atcttgaaaa tgaaaaacag aaacagacgt tgcagtcgaa
     9421 taaaccatca aataaagaag atctcttcgc tcttgttcgg tatacacagc ttcaaatcga
     9481 gtaatatcca aaaatttttc tgcccagtcg attgctcgga atttctttat acggatacgg
     9541 gtatcatcca catattcggc aaaaggagtt aattgtttga tcgcctcgct tgtatttact
     9601 tcgatgatct ctgctgcttt ttctagactg atttctcttg gtgttttcag gagataacta
     9661 attttgtagt tcacagcgct gttcacctcc tacttatatt ctagtatggt agcgctttta
     9721 aatcgagggg tgtttggtac aacttacagt tggatttttt ttacgactgt accgaacgat
     9781 ttctatcgaa tggatacaga aaaaaagcag gtgaccaaat gtctcctgct tttatgaatt
     9841 gatttcgtcc gatcaatgat gatcatgttt attttttgag gtcagttctt caaatttttc
     9901 tttgacatct tcaaccgctt tttcgatatc tttttcaaag tgattgggct cattaggctt
     9961 tgtttcctct tctacagaat ttgaaccaga taaagctgtt tctttatttt ctgtttcacc
    10021 agagtttgtg ccagctaatg attcttcttt gtcttctgtt tcaccagaat tagttcctgc
    10081 taaagccact tcttcttctg ttttaggtgc cattgaacct cttaatcgtt tgtgtttttc
    10141 accgtcataa tttgtcatgt tggttcctcc tagtagttct atactttttc atcgtacaat
    10201 aagacagttt atgcgtcaaa aaacaaaccc gattcttaca aatatgtcat atttttcctt
    10261 ctgattcttg aatgcttttg attccttctt ccaaaatttt catttgtaag attgtttctt
    10321 catctttgat gactttttct gcgccattaa ctaatgtctc gtaaatccct tcgtagacac
    10381 ggctgtaatc accaacctcg gaaattactt tttcttcatg ataactccct tgttcatcta
    10441 tataggtcaa tacaccataa tgttcgggca aatcgatacc aaagtcttca tgatcaggca
    10501 tgtaaaacat tttcagatgt tcttcttgtc gatcttttgt ttgtttcacg aacatccctt
    10561 tttttccgta aacgacgaaa cttggacgtt ctttgatgcg gaaataactg gattttatcg
    10621 aaattttcat atttccatag aaaaaatcca gatcgaagta atcattcatt cgtcctttcc
    10681 ctaaaagttg acgtacatca tagctgatat catctggttc gccgaaataa gaaagaactt
    10741 gatctactgt atgacaagca tgtccatata aatagctgtt cgcagtagaa aattcatgcg
    10801 tattttctgg tacttccggt ctgaaataat cataatgcat ttctacttct aaaatatccc
    10861 ctaattttcc gctttcgatt actttttgga ctgtcaagaa gtcagaatcg aaccgtctgt
    10921 tttggtaaca ttggataaat agaccttttt cttttgctaa tgcaaataac tcttctgctt
    10981 cctttgacgt ttctgcaaaa ggtttctcta ctagtacatt cttaccatgt tcgagtgcca
    11041 tttttccata ttcataatga aattgactag gtgtagtgat gacaactaat tggatctcag
    11101 gatcatccca aagatcagac agctgatcag tatattcgat gtcttcgaat gttttccaca
    11161 ctggttcaga tggggaaaaa atttttttga tattgatttt gttcgtaagt tttcttgaaa
    11221 aaggcaaatg atagcgattt gtactctttc cattgccaag ataagcgatt gtaagcatga
    11281 gtggtgcctc cttttattga tgttttcagt atatcctaga aaagcgcaga aagtatttat
    11341 aatagctatt ctggtaaaaa agttcagaaa aacacttgtt atcattcggt ttggtgaaat
    11401 taatgttcaa agtaaggata aaaatcaaaa agatagtagc ggaaccaagt gtttatcagt
    11461 aagatagaat ttggaggtca gatcatgctt attgaagaaa aactaacaaa acaagagttg
    11521 tttaccacga cagagaaacg gattgtcgat tacatccgta ggaatatcga agctgcagtc
    11581 tacatgacga ttgaagaatt agcaaaagct acgtatactt cccattcagc tatcattcgt
    11641 ttatgcaaaa aaatgggatt cagcggattt aaagaattcc gtttcgagct ggggcgggaa
    11701 gttcatcaat tgattgctca gttcaatcaa acgattgatc ctaattttcc tttttcaagt
    11761 gaagatagca ctcagcaaat tgcaaagaaa atggcggatt tatcgatcca gtcaataaaa
    11821 aaagcacaat tgcagattga acatgaagat ttagatcaaa tcggtaaagt attgaacaag
    11881 gcacagcgaa tctttctttt tgcaaaaggt gattctcaaa tcacggcaag gaaattccag
    11941 aataaaatgg tcaagttgaa taagtttttg atcatggctg aagaatacag tgattcttct
    12001 tggaatgcag ccaatttgac tagcgaggat tgcgccattt ttattagcta cagcggtcgc
    12061 atccatcatt atgaacgaat aatgacctat ttgaagcatg tagaggcccc taccttgctg
    12121 attaccggaa atcagcattc agaaatggcc aaacaggctg atatgtgtct tgttatatcg
    12181 caggaggaat atgattttgc taaagtagcg actttctcct ctcaaattgc cttcgattac
    12241 gtattaaata cgttattctc agtgatatat tctcaaaatt ttgaagagaa tatccgcaac
    12301 ttgaaaaaca aacaagaatt gatgcaaaaa ggccttctta aagaatatcg gaaaacttaa
    12361 gaaaagatcc tcatttagta ataagaaaga tagtgtttga taggctctct ttgatatcat
    12421 cataaaaaca ggctgaagtt ttctttttca aacctcagcc tgtttgttta cggaacgatt
    12481 cgaatagata tattatagaa atgaggaata gctaacttct gtttcttatt ttgaacgatt
    12541 gacgaaaaag atatataaga aggaggaaaa tcagtataca tgcagctaac aatccataca
    12601 ggaaaaatcg tttgttctcg gatactttct gtacttcttt ttgaacttct tttgcatgat
    12661 aaggaacccg gtgccctctg acaagtaagc gatgactgtt gaccatataa ggtgtacaag
    12721 ttaggagtgt cacgaaatcc tgcccttttt cgattctcag atcttttgta tcagtcggtt
    12781 ctaccgtctt gatctgatcg acttgataag cgagtgtccg tccattgatt tcgatataga
    12841 aagaatcacc taaagtcagt ttcggtaagt ctgtgaacaa ctttgcttga ggcagtcctc
    12901 gatggctgga aataacggca tgtgtatttt ctccccccgt aggataagac gttccttcca
    12961 ataatgaggc acctttttct agcagcacag tattcgtttc atcaaaaatt ggcaaatgga
    13021 cattgatctc agggatcgtc aaaataccga tcgtatgttt ctcaaaatag gttctatcct
    13081 ttttcgcagg ttcttttttc ttagtaaagg gatctgcacc tggatttcca ccttcttttg
    13141 ctaattgctg gtttttcttt gtcatgtttt cttggatttt agccatgact tcttcatttt
    13201 ctttgaccgc ttgttgctga taatgagaga tgatctgctg atccagataa ttattcagcg
    13261 cgtcgctaac aaatggataa gctagtatcc ctattcctga gagaagcaag aaaagcatca
    13321 gcagatctag aagtttcctt ttctttggtt tcatttgtgt tctccttgtg atgactgttt
    13381 gataataaaa gaaaaaggag catttgcgta atccgctgct cctttttcca attcttgcaa
    13441 aaagactaaa tctgactgtg cttgcgtcta gcaaagtaca gtccagcaat cagtagaagg
    13501 actactcctg caccgatata cacatagatt cccttaccgc ctgttgaagg aagtgtacct
    13561 ttgtgtttat ttggtatttt ttcaggagaa atcaactgtc ctgctgttac ataagattgt
    13621 tcatcgatag taaatgctac acggtttgtt aatggaacat atttttctgg cgctttcgtt
    13681 tcttccagat agtacgtgcc atattttaga cctgtcacat cgattaaacc gttacttgtg
    13741 gttgtaaaaa ccgttgctga ttctttcgcc gatacccagc tgacggcttt tgtggatggg
    13801 tcgatcgata aatatttcgc tgtgtcacta tcttgatcac gaacgacgaa ttctgctcca
    13861 gcaagtgttt ggtctgatgt aacgtcacca tctactttaa caaatcgttt gcccccagta
    13921 acgacatcga ctgacggtgg tgtttgatca tttgtatgcc cgttatcgac atttgcttgg
    13981 ttagaaaatc ctttggttgg atctgctttt tcgttcaaat gcatatagta aacgaatttc
    14041 aatgtaccgc caggagttaa tgaaggaata taattcggat caactgaaac cgtgaatccg
    14101 tctgtttgct cagtgacaga ataatttact gggtcgattt ctttatttcc atcatataag
    14161 gcataagcat acgttcctga agaatcatta tcaaatgtta aagcagcgtc atgagtatcg
    14221 ataagtttga atgttgtgta cttgttttgc gttccttctt tatcagcgat acctaatggg
    14281 atattgacag aaatctcata ttgaattttt tcaccgattg cgacatcttt tccattcaat
    14341 tgaggtgttg ttttatcaac tttagacgta tcatttttga tggtcttttc tactggtgtt
    14401 tggctatttg ccaggatctc aaaaggcgtg attgtttgct tttcaatcat ttccgcatta
    14461 tcaggagctt ttacttcttc taaattatat tttccaactt ctaaatgatt aacgatcaac
    14521 tggccttttt caatagatgc ctcggcaaag tcattgttgc cgatgtcata agaatgacca
    14581 gtaatgaaat gtttggcttt ggtttgatca gttgtccaag tgtacaatcc atctgtgaca
    14641 ctttggatgt attttttgac gcttggtgtt ccttcttctt tagaaataat aaattctgct
    14701 ccatttagtg cttcgttttc ggcagtaccg atttttgtaa ctttcaacgt tccatcatta
    14761 ccgactgtat ttttagggta gagatggata gtatctagtt cttctgtccc gtatttataa
    14821 gagccatctg cttgtttgat catctcatat acagggaaag ctaaaaccat gtttgcggca
    14881 gctgacactc cgtcttttgg ttcttctttg atcgtgtaga ctgcatcttt cccattttgt
    14941 tttttaggta aagataaagt gacatttcca tcagcatctg tcgttcctga agcaactggt
    15001 gtaccaggag tcaaagactg gactgcttgt tttgctgcat ccacggacgc tcctttatct
    15061 cgttgcgcat aaaattcttg agtgacgtta taaactgaaa atgaaatatc ggctaatcct
    15121 tggtattgat cgaattcgct catttctttc ccgctgtttt ggattaaagg atcgggtaaa
    15181 tcagtcattt tctttttatg aaggatgact tgagctgctg tctcctctgc aaacactttt
    15241 tttgcgccga agctgtttgt gagtaatggt aaaataagga aaaggactcc taacataacg
    15301 tttatttttt tatgattttt cattttttca ctcctgttct ttttttgata aacaaccaga
    15361 ttgcaaagct tgcgaatacc ataccaacaa cgcctaaaag tgtatttttc gtttcgtttg
    15421 tttttggaag aaatggacgg ctattttctt ttttattgac tactttcaat atactgtccg
    15481 cctctttatc ctcagaaaga aaagttgaaa tggtaaacgg aatttctgtt tcacttaaaa
    15541 tataaccaga aggtgcttcg atctctttta atcgataact tcctgttttc aacccggaaa
    15601 ttgaaaaagc gccattttta tcggaaatca ggtgaagcgc atctgattcg ttctttgtcc
    15661 acttgtatcc gttggctgtt tgctggaggt aatttcctgc tttgttttta ataaggaata
    15721 ctgcgccagc taatcgttta tctggtttat ttgtctcgat tttgacaaat tgcttactcc
    15781 ctgtatatac agagactttc tttgtactga caccaaaacc aaaatccagt gttccgttgt
    15841 tgttgatttc cttgttcgcc tgtgctgtac tgcttagctg catctgataa gaaatggtca
    15901 gtttttttcc agcatatttt tccaacgaag ggatcgaaaa taaaacatca aatccgtgag
    15961 gtttcttgtg tatgacaaag ccttctgtca gcttttctcc attcgatgaa atcgttaaac
    16021 tattaggtaa aaacgttaat gcttcatccg cactatctga caattcgaac ttttgatagt
    16081 caaggatatt tacaggaatt gtcgtatgca aggaataagt gattttttca ccaatcgtaa
    16141 aatcatttct aggctcttct aataccttct caaaaggtgg atcagggtag tcgttttctt
    16201 catttttagg atacaaatgg atagttgaaa gtttttgatt gttttgtcca tgtacaggaa
    16261 gaacaactac catattctct gccttttctt tgacgacttc tggtactttg gattcaatga
    16321 aaagatacgc tttatctctt ttttcttgat ctttagcggc cagagaaaaa gaagcgatac
    16381 catcttcgtt gttaagagtt gtagttgttg cttctgcggt aaacataccg gaagacgcac
    16441 cgttttttgc gatctctgct tgtgcttctt ctaccgtttt gcccttttcc cgtagatggt
    16501 aaaaagaatc tgtgacatca taaacttgga atgtgacacc atttaatcct cgatacgttt
    16561 gtaataaagc tttttcttgt ccgtcatttg gatgattttt cggcagttgc ccgtttggga
    16621 aaagcaattt gtgtaaagta atctgaacca tttctgtttc tgtttctgtt gctacctgag
    16681 taaaagctgg tttaaatagt aacaagaaga gacacatact aagccaacca agttttttca
    16741 tatttagctg ccccctcttt cttttttcaa aaaaagatac caaatagaaa acgcacaacc
    16801 aatcatccct actagataga ttcctaaacg gccaattcct cccgtttcag gtaatggtac
    16861 ttttgcctga ttcgtgatgt ctaaagaaat ctggttgttt ttggctcctg gtgacagaac
    16921 agattcatga tcttgtccat ccacttgaat tgacccatct tcgtgtataa ctatagtaac
    16981 tggctctttt agaccttggt atccttctgg tgtaaaagtt tcggtcagcg tataagttcc
    17041 aggttttaaa ttttcgaata gaaaggtatc tgtttcttgt ccatcttttg gcgattctaa
    17101 gtccatttct ggtccctgca gtctgaattt tgctcctttt aatgtctgtc cgttgtcttc
    17161 ttttttgtgt accgttagat caaacgcttt tagtcgattt tgatgagtga gcgtccatac
    17221 attatttgtc atctcggtat tttttcccgt gtaaatgatt tttccatatt tatcgatttg
    17281 gaattcataa tttcccggcg aagtatcata tccggcagga ccagctgttt caacgactcg
    17341 atatttacca ggtgtgagtg aatcaaaact ggcaagacct ttttcattcg ttgtttggct
    17401 gtcaattggc tgataagtac catttgcttc ttttttctgt agggaaaatg ctgctccttt
    17461 aagatttatc tctttgtccg tcccttgcat cgcatactta cgtactgcta caggaacttc
    17521 aacaaaagga ttttcaatag tcaactggat cgtatcatcg gaagtagtga ctgtttttcc
    17581 atcaatggtt accgtaccat cagaagcgat cttgatttcc caagtagtct ttttgcttaa
    17641 tccatgccct tctggagctt ttgtttctgt cagcgtatag gtcatttctt tttgcaattt
    17701 gacattttct ggaagagaat aggttccgtc gccatgatct gttagtaaag tatcaataga
    17761 atctcctgtt aatttgaaaa cagcgccaat aagatccttt tcacctgtag aggaattttt
    17821 cgttattttc aagtttaacg gtgtgaattg tttagtattt ttccatgtca ttgcatctat
    17881 ttgttgagaa tcgtatcctg gtacaggtaa ttctttgatt gtttggtaac tgaatgcttg
    17941 accttgatta ttgtattgag gtagtgataa tatctcttga taactttctc cgctatttgc
    18001 agataatttg tcaatgtctg cacgttccca cgtatttgtt gtatctttag ctggtttaat
    18061 gattcgaata tatccgtttt tccaagctgc agcatttgtc gtatgttccc gttgaatctc
    18121 aaaagtaact tgatctggac gatcagctag attgttgtca aattcttccc ataacttttt
    18181 gatgtgaaga ctgactccgg gagctttagc agatggtata ccgaattcag ctaattcatt
    18241 ggtatcaatg tttggctgga aagttgtcct gccgttcatt tgataccaga aatttggatg
    18301 gaagtcctca ttttctgttt ggattctcac ttggtaatgg atttggattt cttggtcttt
    18361 tcctaaatag atctgattgc tcttgatggt atttccttct atggaaatag atggagtgac
    18421 cgttgtagga cttgtcccca cactcttgac tgaaagtgta ccaggctgat aaatgaatgg
    18481 ttctgcgatt ggatcattta tttgtccatt gcttacagtt gctgagatct gtacagcttt
    18541 tttggctagg tattcagaga tatctgttgc atgatcagct gattcatagt aaagatcgcc
    18601 tttttcatct gatgaaacca tttgtcgcat acgagactct acttctgctt ttgagagacc
    18661 agctgccgga tcgctttgaa gttggatgcc aagaccatgt atttcgattc ctcgttcttt
    18721 gagtgccatc gcttctccga tggttgcgat aaacgtactg tcgattcttc tggatagatt
    18781 gttttggtca ggtgcatcat agattcttga gattagagaa gtatttcccg gccgatcttg
    18841 cgtattagaa aactgagttc cgtaataatt gctgcttgat tgtgcgtgta cccgctgtac
    18901 tttataggaa aatgttggta caccatccgt cagcaaaacg atcacttttt tatgtccatt
    18961 tggaacggat agcatgcttc ctgcatctct tagtgctttt tgtgtaaaag taccaccatt
    19021 tgtccgtgaa ggtgtaatgg attttacttg atttttcact gaatcaaatg aacccatctg
    19081 tactgcaccg ttactgtagc tatatccttc gcttgagtag ccgacatatc ccatattgat
    19141 tttgtctgtg ataccgctat ctgctaaagt atcgacaaaa cgatcgacac caatctttac
    19201 ttcaccgatc cgattattgt cgttcatact tcctgaccag tctacgacca atacgagatc
    19261 aagaggcgtg atatcttttt gtacatttcc acgagcattc aaataaacat caaacaatcc
    19321 gggtgtactt gtttcctttg catactttcg tatcgcatag tctgcttgat taccggttcc
    19381 tccatattcg atatacgaat gggtccgatc attaggatct ccattccaag aatttatacc
    19441 atcccattga tttgttccgt ttttattgcc ttgatgatca agaacatttg tatttcctgt
    19501 aggttgccaa ctggctttag gataagtgcc ctgatcatct gttgtatatt ctgtaggaat
    19561 cgtttgatta gatggcacac tcacagctgg caaggataat gtaggcaatg taaatgggct
    19621 gacagaattt tcgttttctt ttgatgattc ttcttctatt tcgtctggct ttggtactga
    19681 cacatctgta tctttcttat tttctgtctc atcaataatg tcatattctt tattatcgac
    19741 gatcagcttg tttgcctggc tgtggtaaag tgctggatcg attggaaaac tcagatcgat
    19801 agtttgttta gtgtttgatg agatttccaa cgtaattttc ccgctattta cctgataagc
    19861 atagccatct ccttcttttc tttccacatc acttaagtaa ttttcgacta gaccaatttc
    19921 tttttctatc ttttcagcac tggtgttatt cgcatttagc ttgatgttca gtgctttttt
    19981 tccagactga tccgaaatga cttcagctga aattgtttct aaaactgttg gatcatctgt
    20041 agtgtctgct attgcagata atggcacaaa gtttgataaa ctcaatatta tcagcatgaa
    20101 aataactttc agtttcttcc ctgtggtcgt catttttccc ctcctttttc tttttttctg
    20161 ttttgacgtg aatagtgaaa attattaaaa taaacaggcg ttacataatt ttatttattt
    20221 taatatgata attattatac gaagggcgct aaagggaatc acaccaattt tcataccaat
    20281 tggtttcatt tatgaagttg attccttcga ttaatgagcg tttcattgaa agaaaagggt
    20341 tgagggagaa aaacatatga tcaggttgtt ggaaggaaac tatagaaaga tggaaaaaat
    20401 cattcgaatt ctgtccattt ctgacaagtg gatgactaaa aaagaacttg ccaaacgaat
    20461 tggtagttca gaatctacat ttgtaaggta tatcgaagag atcaaaatcc gctggggaac
    20521 ggatgtaacg attcaatctt cacaaaaatt ggggtatcgt cttgaacatt tgaatgtacc
    20581 agcttattta aatgttctga tggaaatgtc gcaaagttct acaaacagtc gtttattaaa
    20641 tgagattatt gtcaatccgg gtaaaacgat tgagtattat tgtgaaaaaa tttccatcag
    20701 ccggtcttct tttacacgta agttgaaaca atgcaatcaa attttagaaa gctatttatt
    20761 aaaagtgatc gttgaccaag gttttcagtt aactagcctt aaaagtgagc tccagttgcg
    20821 tatctttgtt acctttttct ttttaagtta ctacggaaga cgcacgttgc catttccaat
    20881 cgaaaaaaag aagatcaaag aaattatggt caagaataat tgtcagatga atttgtttca
    20941 ggaagaaagc ccatatgagc aatctttctt tattatgtat tttgttgtgc atctcatgag
    21001 agaatcccaa ggaactccat ttgaatggaa agctggagac tcttatgtac agttgataaa
    21061 tatcgataaa aaagacttct taatcttgaa aaatctctta ggaaaattga agtacagcag
    21121 ctatataaaa gtagtgaact attttgtcaa tacgattttt tcagaatttt cggagaaaga
    21181 aaaaaatgaa atcagtgagc agatacgaaa aaatatgtcc tactgttcgt tttcatccgc
    21241 tgattgcaaa gaaaaaaacg aagaatatgt tgttcaatta ttaacgcaaa tttatttttt
    21301 ctcccgtatt ttcccatacg atacgactca tttgacgatc cgtgcaactt actttgcaca
    21361 gcagatgcaa caccgccaga tacgtctata tgctggattt cgtgcagaac ttcaatttta
    21421 ttccactcta ctgaatcaga atctgattta ttatttatct agctatatct tttggatgct
    21481 tatcggtaat cctgaaattc accattatac ttctgataaa aaaatactga ttatcagcga
    21541 cttaagtctt cgacatagtc aatatattga agaatatatc agtgatattc ttgctgtgca
    21601 taaaattcac tctgagacaa ctgctattac tgaagatcag ctgtcgaaat acaatcttct
    21661 cgagtatgat ctgatcgtta cgaatcaacc cattttgaat gcccatgttc ctcatatatt
    21721 gattgatgat agtgtgtcat tcgcaaatga agaagaactc atccgcttat tcgagttatg
    21781 aataatcaaa aaacagctat gtataaacca ccctgataaa aatttcagga caaatacaca
    21841 tagctgtttc tttttattgt attccttcag caatccaacg agatttatag cgaaagtctt
    21901 ttaacaactc ttcttttgtc caattatttg caatgagatc cgtcaaagtg ttcattacga
    21961 gggtcatgga aaagtatagg aaactaactg cttcttccga cgattgaatg gagaaatgat
    22021 tgattcttaa taattccagc catttttcgt tcattggaga atcagccgat agttctctac
    22081 gtttggttgt ctgctgatca tttcgctggg ttaacaagta taacctttgc caatagatac
    22141 tttcgtgatc taagtcgcag caccactcta aatatttttc gatccccaaa aagaaatgtt
    22201 gtttgttttc gctaatgaat tttaagatat cttgatggac tatgtgagaa ctgcttttga
    22261 tcgtatacaa ataagcatct tccaaatctt gaaaatattt ataaaaagca ccgcgcgaca
    22321 tccccatctt gtcaacgatt tctgagactt tgacttggct gatgtgctgt gagcaaaata
    22381 tctccagtaa taggtcatcg attctctgtt tctttgcgtt cgataaattg aaaaaagttt
    22441 cttttggcat ccacatctct cctttatctc tgattatacg tggatagccc ttcattgtca
    22501 tgaaggattc attccttctt tttctcttct tctgtcaagt acccatcttc cattcgataa
    22561 acgcgatcac tccattgaat catacggaca tcatgagtaa ccataatagt cgctttttgt
    22621 ttctcatgag cttctttaac tagtaatttg acgacttcat atgcatgatc tgtatccaaa
    22681 ctagctgttg gttcgtctgc taagatcaag cttggctcat tatataaagc gcgagcaatt
    22741 gccactctct gtctttctcc accagaaagg tctttaggat aactattttt aagtgactgg
    22801 atatctaaag attgcagcaa ggaatcgatt tttgttgaat cttctttttt tgaagtaact
    22861 tggtcaacta gttcaaattg ttcctttacc gttaaaaaag gaatcaaatt agagctttgg
    22921 agaataaatc ctatttcttt aaagcgtaaa tctgctcgtt ttttttctgg aagctcagtt
    22981 agtgagtgac cgttgatcac tatttctccg gaagtcggtg tttgcaatcc tcctgaaatg
    23041 gtaaggaaag tacttttacc tgaaccagaa ggaccaatga tgctgacgaa ttctccttcc
    23101 tttacataaa aatcaattgc tttcaaagct gagacctttg tatgaccaga accaaatgtt
    23161 ttggtaactt ttttcatcgt taatattttt ttcattttta ttaacctcct attgctgtaa
    23221 ttgggtctac tttggttatc gtacgtatcg aaaacaatcc gcctagcatt gcgacaatga
    23281 tcaaaatccc actatatagc aaccattgtg accaaaaaac agcaaaaggc atcgcgcttg
    23341 gtaaaatcag actagtcaaa taagcaaaca aaagagcaag cagaacgcct accaccccaa
    23401 caataaagga ctgtgctact aaagatttag cgatgaagaa atttttgatc ccttgtgctt
    23461 tcattactcc aaaaatagca gtcttctgaa gtgtcatcac gtacatgaaa ataccgacca
    23521 cggcagcaac cactaagaac aaaaagtata tcatcgcatt taaggtcata ttttgggcag
    23581 aatagccagg aatactttca atgaatgtcc caatagttaa taactgtaga cccttcgatg
    23641 ctttttcttt cgaaatttta gctgaatcct ttattacgat cccattgatc ggctgttctt
    23701 cttcagaagc aaacggctgt tgtccatact tcaaagcagt ccacgtatct agggacgcat
    23761 agatgacggg agaaactgtg taataagttt caggaaagat accgacaatc gtcaaaggat
    23821 cgtcgtaact gccaattttg atcgtatctc caattttata gcctgaatca gccagatttt
    23881 gagagatgat gatttcattc tttgttttaa attcatgccc tttcgtgagt tttggtaata
    23941 aaaatgcttc tttcgttgtg ccgaaaactg taacattttc tttgtttttt cctttgattg
    24001 ctccgctgta caatccgatt ggtgctttct ctcctccttc aatgtatttc agatctcctc
    24061 ttgttaattg tgaagctgca aatacttgat ttgcatcctc cgacaaaaca atctcttgtg
    24121 cgtcccaatc atcgattgct tttttaaact cttctgaaag tccattcgct agtcctgaca
    24181 acatgaatac gacatacgca atcaagaaca taataccgac gatcaagcca tatcgtaatt
    24241 tagaatagcg aatttctttg atagctagaa acatttgttt ttcctcccta tgatagattg
    24301 atagtgacaa aatgtcactt gttgaatagt gtaactctaa agtgacaatt tgtcacttgt
    24361 gttgtttttt tcttatcaat aaattgtcta agacatcttt cgataaaacc ttaacatgag
    24421 tcataaaaaa tttatttaag ccttatttat gctttccatg ataaactaaa gtgaccaaat
    24481 caatgaggtg ggaaaatgat taaactatta gtagtagaag acgataaagt attatccaat
    24541 aatataaaag aaatactcgc tgcaatcggc gagataacac aagtttatga tggtagtgaa
    24601 ggactgttcg agttggaaag tggagtttat gatcttgcaa tcttggattt gatgatgccg
    24661 gaaatgaatg gatatgaagt gttagcaaaa atacggaaag aaaaaataca gactccagta
    24721 ttgattttga ctgcaaaaga tagtttggaa gataagattg aaggatttga acgaggtgca
    24781 gatgattatc taacaaaacc tttctatcga gaagaactga tcatgcgcgt gaaagcatta
    24841 ttgaaacgct cgctaggtct ctttgatgaa aatgttctga agtataagga tatcagttgt
    24901 gatttaaaaa gcaatgaagt cttgtataaa gaacaagttt tgcccataca aggaaaagag
    24961 tttgaattgc tcgtttattt tttacaaaat aagggaatca ttttgacaaa agagcagata
    25021 tttgaccgga tctggggatt tgattctgat acgacgatta cagttattga agtttatatg
    25081 agcaacttac gaaaacatct gcgaagtact ggattggata aagaaatcaa aaccttacgt
    25141 aatgtcggtt atatactgca aagggtgtga tgcaatgaat ggagatatta cacgaaagca
    25201 acgtagtcgc ttttttgcga caaatgtagc agcatttgct tttatctttt ttcttttggg
    25261 attcattact ttacagatat taaaccaatc tgcatacaga gaaacagatc tgtcattaga
    25321 gaaaataaat tttgattctc gaacagtaca attagagata caaagatatc agcaaggcga
    25381 tcccttttta aacccgactc aaacggaaca aaacagaaca aacatggaac agccattaat
    25441 ggaaccatca aatgaccgtt ttaacactca agtgatcttg tggtctaaaa atggtgagat
    25501 attgaataaa gcagctattg gtgggcgttt caaccaaatc aaagaattaa aaatggatac
    25561 gaaaaatctg aataccatcc aaaatattga attaaatgaa gacagtggct catctgccct
    25621 ctctttccgt tcgattacga aaaaagcgga gacgaatcaa gatggcgttg cctacatcca
    25681 aattttagaa aatacaaatc aaatcaagaa ttcattagcg acttttcgca cgattatgat
    25741 tttgtgtatg attgtttttt ggattctctc tattggtatc agttattact tgtctagtct
    25801 aagcatgcgc cctattattc tttcttggag aagacaaaaa gaatttgttg agaatgcttc
    25861 tcatgaattg cgcacaccgt taacgattat tcaaaatagt ttggaacatc tttttaccaa
    25921 gcctaatcat actattattg aagaatccga aagcatcgct caagcattgt cagagacgag
    25981 gcgcctcact ggattgactt cggatttgct gacaatcgct cggaatgatt ctgatcagca
    26041 gctgctttca aagcaaatga tcaatacaca agaatatatc gaacaacttg ttaaaccatt
    26101 tcaagagatg gccatcatag atggaaaaga gtttattctc cataacgatg caacattaga
    26161 agtaccaatt gatgaaaaaa aaatccatca agttttggtt attttgctgg ataatgcatt
    26221 aaaatatacg aatccagaag ataagatcat tttggattca tctgttaacc ataaagaatg
    26281 gctgattcac gtaaagaata ctggcccttc tatcagtgaa gaagatcaag caaggatttt
    26341 tgatcgattt tatcgggaag atcaatctcg ggcgaaagag actggcggat acgggcttgg
    26401 cttggcaatt gcaaaacagg taatcgaaca gcatcatgga cagatttctg tatccaatta
    26461 tcttccacaa ggagtcgatt ttaaagtcca attgccgttg aagcctaaaa aataatgctt
    26521 atccctaaaa aagggctgtg acaaaaatct tgtcacagcc cttttttgtt gcgaataaac
    26581 ggtgttccag aagtagcttc ttcggaaata agccgaaatt tctttaagct gaccacttct
    26641 gtcacaacct ctttagctta cgcttacgat tttttattgc ggtcttcaat gtgtacatca
    26701 ccgcttgtgt caatatccaa ttcttctcgg ctaacatctt ctgtcacttt ttcaacatct
    26761 tcatgttctt ctttatgaat cccaacttct tcacgaataa ctgtatgttt gcttacatct
    26821 acttgctctt ctttgattgg gatagtaatc gtgtcatctt ccaatttttc gtctgttccc
    26881 tgtgaagatt gatctgttac tggtttgcgt tcgatgacga tttcttcacg tttgaccggt
    26941 acttctaccg tttcagtatc gttgaccaca tgtttgtgga tgtcgacttc tccagttgtc
    27001 acatcatgtg tatttacatc taatttttct tcttttagtt gaattgtatc atcatctaga
    27061 tttggtttgc gatcgatgtc ttcagtcttg cttgtaccca ttgtatcagt ctcgccaggt
    27121 acgtccatcg gatcagttcc tggtgtcata ttcaatggat ccgttgtatt ttgaaagcct
    27181 gtcgtgccac ctacgtccat tggatctgtt ccagtgccag tttctaatcc agtccccatt
    27241 gtgcttgctg ggctaacagt atcttgtgta tccattgtat cttctgttgt ttctggctga
    27301 cgatagcctt ttactgtgat gacaaatttt ccatcagaaa tatctcctct atattgatat
    27361 aaaggatcat tttctggtgt agccgtctca gaatcatagg tatcaaaaga aaaagtatct
    27421 tttatttttt cccataaaga acggtcttct tcaccatcca tccgattttc atctgtttca
    27481 acatctatac cagacaatcc gtcaaatcct agtctagatg ctgcttcatc agtagtataa
    27541 ataacgatat catctcgttc atacccttcg ttacgcaacc gttcaattac tgataatgct
    27601 tcttccctcg tactatagct tccgatgacc gttaaatttt tctcttccat gtaaattcct
    27661 cctagcaaat agatttatga tt
//
LOCUS       DAJSKS010000021        26990 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJSKS010000021 DAJSKS010000000
VERSION     DAJSKS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.021"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            113..1183
                     /locus_tag="ON396_000803"
     CDS             113..1183
                     /locus_tag="ON396_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3112930.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            1198..1866
                     /locus_tag="ON396_000804"
     CDS             1198..1866
                     /locus_tag="ON396_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3112931.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            1886..2464
                     /locus_tag="ON396_000805"
     CDS             1886..2464
                     /locus_tag="ON396_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3112932.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            2865..3776
                     /locus_tag="ON396_000806"
     CDS             2865..3776
                     /locus_tag="ON396_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR3112933.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(4154..5071)
                     /locus_tag="ON396_000807"
     CDS             complement(4154..5071)
                     /locus_tag="ON396_000807"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCR3112934.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            5273..5710
                     /gene="zapA"
                     /locus_tag="ON396_000808"
     CDS             5273..5710
                     /gene="zapA"
                     /locus_tag="ON396_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR3112935.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            5768..6316
                     /locus_tag="ON396_000809"
     CDS             5768..6316
                     /locus_tag="ON396_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCR3112936.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            6431..8791
                     /locus_tag="ON396_000810"
     CDS             6431..8791
                     /locus_tag="ON396_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCR3112937.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            8878..9192
                     /gene="trxA"
                     /locus_tag="ON396_000811"
     CDS             8878..9192
                     /gene="trxA"
                     /locus_tag="ON396_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCR3112938.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            9374..11152
                     /gene="uvrC"
                     /locus_tag="ON396_000812"
     CDS             9374..11152
                     /gene="uvrC"
                     /locus_tag="ON396_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR3112939.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            11297..12196
                     /locus_tag="ON396_000813"
     CDS             11297..12196
                     /locus_tag="ON396_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3112940.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            12189..13451
                     /locus_tag="ON396_000814"
     CDS             12189..13451
                     /locus_tag="ON396_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3112941.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            13612..15510
                     /locus_tag="ON396_000815"
     CDS             13612..15510
                     /locus_tag="ON396_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HCR3112942.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(15840..16559)
                     /locus_tag="ON396_000816"
     CDS             complement(15840..16559)
                     /locus_tag="ON396_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HCR3112943.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            16896..17228
                     /locus_tag="ON396_000817"
     CDS             16896..17228
                     /locus_tag="ON396_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3112944.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            17215..19218
                     /locus_tag="ON396_000818"
     CDS             17215..19218
                     /locus_tag="ON396_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HCR3112945.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            19237..19707
                     /locus_tag="ON396_000819"
     CDS             19237..19707
                     /locus_tag="ON396_000819"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HCR3112946.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            19728..20312
                     /locus_tag="ON396_000820"
     CDS             19728..20312
                     /locus_tag="ON396_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HCR3112947.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            20327..21313
                     /locus_tag="ON396_000821"
     CDS             20327..21313
                     /locus_tag="ON396_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HCR3112948.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            21303..21614
                     /locus_tag="ON396_000822"
     CDS             21303..21614
                     /locus_tag="ON396_000822"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HCR3112949.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            21636..23417
                     /locus_tag="ON396_000823"
     CDS             21636..23417
                     /locus_tag="ON396_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HCR3112950.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            23410..24786
                     /locus_tag="ON396_000824"
     CDS             23410..24786
                     /locus_tag="ON396_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HCR3112951.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            24793..25428
                     /locus_tag="ON396_000825"
     CDS             24793..25428
                     /locus_tag="ON396_000825"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HCR3112952.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            25458..26813
                     /locus_tag="ON396_000826"
     CDS             25458..26813
                     /locus_tag="ON396_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HCR3112953.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT         8913 a         4567 c         5898 g         7612 t
ORIGIN      
        1 aaaaattcat ataatgaaat agcggaagaa ttttttgagc ataatattcg ccaaaaattt
       61 atcaagtgat aaaattgttg tataaaatga ttatttcagg aggaggagtt aaatgttttt
      121 agcattaaat gaaattatgc attcaaagtt acgttatgcg ttagttgcag gggtgatgtt
      181 tttgatcgcc tacctggtat tttttttgac tgggttagct tatggcttag ctcaagataa
      241 tcgaacagca gtcgacaagt gggaggcaga ttcgattgtg ctttcgaaag atgcaaattc
      301 aaatctaggg atgtccatga ttactaagaa gatagcagaa gaagtcgaag gtgggaaagt
      361 ggcgtatctt gcccaaacac ctggagtcgt gactagtaaa gacagtacag aagaaggaaa
      421 aatcaatgtt agcttcttcg gtatcgataa aaatcaattc atcatgccca atcttgttga
      481 aggaaaagct tttgataacg atgatgaggc agtaggcgat attagtttga aagaagaata
      541 tggtctagcc ataggagata cagttaagtt atctggtagt gacaagacgt ttaaactaac
      601 tggctttacg gatcatgcca aattcaatgt ctcaccagtt ctctatacaa ccatcaatgc
      661 ttaccaagaa atccgcttcg aaaaagaaga tacaagtgaa aatgcacgga ttaacgcaat
      721 agttgtcaga ggtaaaatca gtaatttacc tgaagattta gaacagatca aaatttctaa
      781 attcatcaat gaattacctg gatataatgc gcaagtattg acttttggat ttatgatcgg
      841 atttttgatc gttattgcgg ctattgtgat cgggattttc atttatgtac tgacgatgca
      901 aaagattaat atctttggtg taatgaaagc acaagggatc acaggaggat ttattgcgcg
      961 ttctgtagtg gcacaaacat tcattttatc atttgttggt attcttcttg gtctattagg
     1021 aacagtggga acatcccttg ttctacctga tgcggttcca ttccaaagca attggctgtt
     1081 ttttggcgtc attagcttgt tgatgttagt agtagctgtg ttaggcgcac tattttctgt
     1141 tcggacaatc gtaaaaatcg atccgttgaa ggcaattgga taagaaggag gaagaacatg
     1201 aaagcaatag aatttaaaga agtagaaaaa agtttccaag atggcgacca agtagttaaa
     1261 gcattgaaag aaacaaccat gagtatcgat aaaggggaat ttgttgcaat catcggtcct
     1321 tctggttcag gtaaaagtac ttttttgact ttggcaggcg gattgcagac gcctagcagt
     1381 ggctctgtat ttatcaatga tcaagaattc agtaatcaaa aagaaagcga acgcgttaaa
     1441 ctacgtttta aagaaattgg ctttattttg caagcttcca atctggttcc ttttctgaca
     1501 gtaaaaaagc agttattatt agtagataag attaaacatg aacatcgtca aaaagaagca
     1561 aacgaattat ttgagcagct tggcgtggat aagttgatca acaagtatcc agaagaacta
     1621 tctggtggag aacggcaaag ggtcgcaatc gctcgtgcat tgtacaatga tccgtccatt
     1681 attctagcag atgaaccaac tgctagtttg gattcagaaa aggcacgaga agtagtcgat
     1741 atcttggcaa aagaatcaaa agacaaaaat aaagcaacga tcatggttac tcacgataca
     1801 cggctgattg acaaatgcga tagaatattt atgatggaag atggtgtatt gaaagaaaaa
     1861 gaataaccaa aaagcaggtg gaaaaatggt ccatcgatac aacaaagata cacagcaaaa
     1921 aattttgaaa gaagcgactg acttgtttat gtccaaggga tatctcggca cttcaacaag
     1981 agaaatcgca caaaaagttg ggatcaccca gcccaatctc tatcattatt tcggtgataa
     2041 agaaaaacta tatacggcag ttttagaaaa ccacttaaaa gatgttggaa aagctttgag
     2101 agaaatcgtt caaacaagcg aaacgggatt tcaagtaacc ttgacaaaaa tggcgcagtt
     2161 tttgattgag acacatctgg tcgatttgtt tctgatgctt cacgatcttg aaagcaatct
     2221 ttcaaaggaa acaagagatc atcttttctt tctatggaag aaaaattatc gtgaaccttt
     2281 tgaagaaatc ttttcagcaa accaatctgt gctaagaaat gggatttcaa aagaaatcgc
     2341 agcaaggcat tttttcttag tgttagcacc ttacatcact cggtcgaaaa gacctgtgga
     2401 agaaccgcta aatgtggcgc aattgatcga tttatatttg aatggtgtat taggagaagc
     2461 gtaaagctgt tatataggga gaaaatagtt aggtgaagaa gcaagatatg acgaagatcg
     2521 ttatatcttg cttctttata taggtagttt ttcataaata caaatttgtt attctctcat
     2581 gattatcagg gtatattttt atttattatg tcggtgttcc aggaagtgtt ttaatttaat
     2641 aacgaagaaa ttaattgttt gtagaatgaa ggaaactatt tattgggtag atgaatcacc
     2701 acgaatagtc aaaaggagat ttcaggatat gccaaaaatg aaaaaaatca ttttagcagt
     2761 aatgttattt tctacggtta tatcttttac agcccctttt ttgcttactt aagctgattt
     2821 taagttttgg cccttagtct ggatctacga ggtgagagtc aagaatgaaa aaaataattg
     2881 gagcttttgt atttgcaatc attatcatta ttatctgttt tcaaatagat gtttttggtc
     2941 aaaaaaagat agttggacaa acggtcagca gtacgaacag tacggttaca aagacaaagg
     3001 aatcatttga tgatacagaa caagagacat taatcaaaat cggaaaaaat gaccgtacac
     3061 tatatacaga tgactcgcta actaccccac ttgcaactat aaacggcgga gagctggcgg
     3121 agtttctttc tgaaacaaaa aatgcttatc agatcaagac aaatgatggc tatactggat
     3181 atttagccct cgaagatgga accaaagtca caaagaatat acaaacaaaa ccggaagagc
     3241 tatctgatgc agtgattgta ttagatcctg ggcatggcgg aaatgatact ggggcattaa
     3301 gcaatgatga acaaacagaa gaaaaagata taacattaag tacagccata aaagtaaaaa
     3361 aggcgctaga ggatgctgga gcaacggttt atttgaccca tactacagat gaattagtac
     3421 agcttggaga tatatgcgac tacagtgaag aaaaaaaggc agatgtcttt atcagtttac
     3481 acgcagactc tacggaatat gcgaatgaag caactggaat cacgacatat tattactatg
     3541 gacaagagga aacattagca cagacaattg ctgactcctt cactgatctg cctctggatt
     3601 caagaggaat ctccactggg aactatcaag ttcttcgaga aaatcttcaa ccttctattt
     3661 taatcgagat gggttatatg aataatgata gtgatttaaa ggaattagta acagacagtt
     3721 atcagcagca gattgctgca agtttgacac aagcattgac cacttatttt cagtaataaa
     3781 taagcccagc caaacaaaaa tagctcctca tatttttcgt ttggccgggc ttattatcgc
     3841 agatgcaagc ttttaaacac cgtttaagga agaggtcgtg agcctgccgt tcaactctga
     3901 gtcacaagga gcaatttcaa aaaacagctt ctcatgtttg atgactattg tgacttgtga
     3961 ccgaggagtt ggcatgagaa caccgtttaa gaaaaaggtt gtgaaatcaa cgttttgttc
     4021 tgagtattag agaaacaaac ggaaaaatag ttcctcatat tttgccgttt gttgagctaa
     4081 taccgaggat cagatgattg aacaccgttt attcggaaca agggactgtg acatgacttt
     4141 tgtcacagtt ccattatttt tttagtagct tcaaagcttt ttctgtattg gcaaaatgaa
     4201 gtttggcata tttttcaagc ataggcatgc cgcctttctc caatagtttt gccgctactt
     4261 ggtcagattt cgttccagct cctgacggaa gggtgattcc agcttcagct gatgctttgt
     4321 ctaactcttc caaaaagctg gcacgactga tgatagaagc agcagcaaca gctaaatgat
     4381 attgttctcc tttagtaacg aagtacagtt tttcttgtaa ttgatttttt tctaaacgcg
     4441 catatttttt aaaatttgct tccggcgtaa actgatcgat caaaatcgct tctggttttt
     4501 ctggctcgat tttttttagc agcaaatgga tcgcttgatt gtgcaaagcg actttcatcc
     4561 gaacggcatt gtattttggt tggatctcat tgtatttttc tggagataag acaagcagtt
     4621 tataggggat ggtttttttt aagatgtcag caagctggat gatttggcta tcttttaact
     4681 cttttgaatc tctaacacca agtttacgta attctgcaag ctgatcacgt ttcacataag
     4741 cagcacatac tgttagcgga ccgaaatagc tgccatttcc gacctcatca ctacctaaaa
     4801 cggataattg actgatattt tttggcaaag aagaggctgt ttttgctggc ttggcttgtt
     4861 tatcctgtgc ctccccccat ttatcagctt ctattgctgc attcgtacct tgaaacatca
     4921 cttttccgga tgtataagcg gtaatggtcg tcccgtcttt ttttgcagca aagctagtat
     4981 aaggaatctg tttatttaac aaataagagc gatagtatgc tttcatttga ctaagttggt
     5041 cgtttgttgc tttgataatg acgttgctca taaaattctc ctcgttttta tacataaaac
     5101 tataccgcaa ctcacgactt ttcgttttct aagagattgc gagacatagc atttttttaa
     5161 agtttatcat ataatagtta agtatgtgaa aaatatttgt ggagaacggt gaaagaacca
     5221 acttcatggt acactaaata aaactttcat caaataggag gaaatgattc gtatggcgca
     5281 cgaaaaaaca cgctacaaag cggtgattgc caaccaaaca tatactatta tcggacggga
     5341 aaccaagcat catatggata tcgtaacgaa gctgatcaat gaacagttag cagaattgaa
     5401 acaattatca ccacaaatgg ataatgaaca agcagcaatc ttgatggctg tcaatgcgct
     5461 ttcggatcaa ttgaagaaac aagaaagaat attggagctg gaagaagaaa cagcagaatt
     5521 aaagaaaaaa atgatcaaat ttacagagct tgaaaatcgt gtgaaacgca ttgaagcaat
     5581 cgagaacgaa gctcgggaag tattgaaaga aaatggtcaa gcagatcata ccattaaaaa
     5641 tcatgtggaa gcacaacaga ttttaaacga gaaacgaaaa ggacaaatta aacaaaaatc
     5701 ttctaattaa gatcctttta tcaaaataag aaaaatttaa aaaagttaga agagaaagga
     5761 acgtagcatg ctgagtttgc tgattttatt tttattgcta attgcgtttt tttctggagc
     5821 acgtcgcggg tttgctttgc aaatcatatt cgcgattgga tatgtattgt catttattgc
     5881 tgcccagcat ttttacaaac cattagcaag tcatttggaa ttatacattc catacccagc
     5941 tgtcacccca acatcaaagt tagcattttt tgatcaggta tttgcctttc acctagatga
     6001 agctttttat gcaggaactg cttttctgat tattttgcta attggctggc tgcttacccg
     6061 atttgtgggg gtttttgtcc atggattgac gtatgtgccg atcttgagac aggtagactg
     6121 gattgcagga ggcatcctgt cactaatcat ggcttacgtt actatatttt tgattttgca
     6181 gttacttgcc tttgttccat tagatgtggt ccaaaatcaa ttcggcgagc atagtttcgc
     6241 tcgttttatc gttgagagaa cgccatttct taccaacaag atgtatgagc tttgggtaac
     6301 gaatattttg aattaaggaa aatagtgctg gaaaatattt cgttttttgt acagagtagt
     6361 ttatttaaga aggctgcgta gcgtatgtcc ggcacagctc ttttttcata gtgaaagata
     6421 aggtgataaa atgaataacc gaatccttga aacattggaa tttgaaaaag tcaaacagat
     6481 ggtcagacaa tttgtagtga ctgcacaagg aaaagaagaa ctggcagaat tggtaccagt
     6541 gagtgagaaa caaacgatca caaactggct tcaagaaact gaggatggat tgaaagtgca
     6601 acgtctgcga ggcgggatcc ctatacctaa aattgaaaat atccgtcccc atatgaaacg
     6661 gattgagatc ggagcagact tgaatggagt ggagcttgca caagtttcca gagtattgtc
     6721 tacgacaagc gaattgaatc gtttcattga tgatctttct gacagtgaaa tcgaatttgc
     6781 acgtttgtat atgtgggcag atcagttagt cacgatccct gttttgagcc gccgtttaaa
     6841 ggaagcaatc gatgaagacg gacgcgtgac tgatgacgct tctcctgagt taaagagcat
     6901 aagacagaac attcggcgaa gtgaacaagc tgttcgtgaa cagttagatg ggatcgtccg
     6961 tgggaaaaat gcaaagtatt taagtgatgc gatcatcacc atgcgaaatg accgctacgt
     7021 tatcccagtc aaacaagaat accgcggtgt atttggcggt gtagtccacg atcaaagtgc
     7081 ttcaggtcag actttattta ttgaaccaaa acaagtggtg gatttgaata atcgtttgcg
     7141 tcaataccaa atcgctgaac gaaatgagat tcaacgtatt ttaagtgaac tttctgctga
     7201 acttgttccc catcgtcaag aaatcatcca caatacttat gtgattggta aaatggatct
     7261 gatgaatgca aaagcacgat ttggtaaaga agtaaaagcc atcgtgccgg gaatcagtga
     7321 agacaatcat gtcgtcttga aacaagcaag acatccactg atcgatcagg aaaaagtagt
     7381 ttctaatgac atcacaatcg gaaaagacta tcaagccatc gtcattacag gacctaacac
     7441 aggtgggaaa acgatcacgt tgaagacttt aggtctgtta caactgatgg ggcaagcagg
     7501 attaccgatt cctgcaggtg aagaaagtca aatcggtatt tttgaggaag tatttgcgga
     7561 tataggagac gaacagtcga ttgagcaaag tctatcgacc ttttcttctc atatgacgaa
     7621 cactgtagat attttatcaa aagtcaatga aaaaagtctg gtattatttg acgagctcgg
     7681 tgcaggaaca gatccgcaag aaggtgctgc tctagcaatc gcaatcttag atgatcttgg
     7741 taaaaagtct gcttatgtga tggcaacaac gcattatcct gaattgaaag tttatggata
     7801 taatcgggca aatacgatta atgccagcat ggagtttgat gtggatacat taagtccaac
     7861 gtatcgtttg ctgattggcg ttcctggacg aagcaatgcg tttgaaatct ctagccgatt
     7921 aggtcttgat acagaagtta ttgatgaagc gaagcagttg atgaatgatg agagccaaga
     7981 tttaaacgaa atgatcacag atttagagaa tcgccggaaa atggcagaga ccgaatatct
     8041 ggaaatgcgt cattttgttt ctgaggcaca agaacttcac gatgatttga aagaagctta
     8101 cagctatttc tttgaagaac gtgaaaaaga aatggaaaaa gcaaagaaaa aagcaaatga
     8161 agtcgtgtca gaggcggaag aaaaagcaga aaaaatcatt gctgatattc gtaaaatgca
     8221 acagcagatt gggcaaggaa atgtgaaaga gcatcagttg atcgatgcaa agacccaatt
     8281 agcaaatcta catcaagaag aaactttgaa gaaaaataaa gtattgaaga aggcgaaaga
     8341 acaaaaaacg ttgaaaccgg gagacgaagt gttggttacg acttacggcc aaagagggac
     8401 gcttttacgt aaaaacggga atcagtggca agtagaaatc ggtatattaa aaatgaatgt
     8461 gtccgaagac gagctgacgc ctgttgctcc tcaaaaagag ccgacacaac gagttatcca
     8521 tgctgtacga tctgaatcta gtagtcacgt gccgaatcaa ttggaccttc gaggaaaacg
     8581 atacgaagaa gcgctaagtg aggtagatca atatttggat tctgccattc tagctggtta
     8641 tcctcaagtg actattgtcc acggaaaagg aacaggcgca cttcgcaagg gaattactga
     8701 ttatcttaaa aaccatcgga gcgtcaaaag ctttgagttt gcaccggcaa accaaggcgg
     8761 aaatggtgcg acaattgtta aatttaagta aagcttacta aaggtaagca gatggttcgc
     8821 tatccaagta tagaaatgct atactagtca tagattttaa aaatggaggt ctttgatatg
     8881 tcacaagtaa ttacagataa gacatttaat gaagaaacag ataaaggact tgtattgatt
     8941 gatttttggg caacttggtg tggtccatgt cgtatgcaag caccgatttt ggatcagttg
     9001 gaacaagaat atgatgaaga agaattcaga attgctaaaa tggatgtgga tgaaaatcca
     9061 gaaactcctc aacaattcgg tatcatgagc attccaacat taatgttgaa aaaagatggt
     9121 caagtagtag aaaaagctgt tggtgttcat tcaaaagaac aactacgtca aatgatcgat
     9181 caatatttat aaaaacttac agcaaaaata agctgagggt gggactgaat ccagttccgt
     9241 cctcggtttt tttgcggttg agttaaggtt attcaaatta atattttttg agtataatta
     9301 cgttgtcagg ctggattgtt tttttgacag acatgctata ataactttaa ttgaatatga
     9361 aggggagtac cagatgaacg aacgaatcaa aaacaaatta gctttacttc ctgatcagcc
     9421 cggctgttac ttgatgaaag ataaaaatgg aacgatcatt tatgtcggca aagccaaaat
     9481 attaaaaaat cgtgtccgct catattttac tggtagccat aatacgaaaa cggaacggct
     9541 tgttagtgaa atcgaagact ttgaatacat cgttacagaa tcgaatatcg aagcattgct
     9601 cttagagatc aatctgataa agaaaaatga tcctaaatac aacattatgt taaaggatga
     9661 taaaacctat cctttcctta agataacgaa tgaaaaatat ccccgtttgg tgattactcg
     9721 taaggtacta aaagacaaag cactgtattt tggaccatat cccgatgtcg gtgcggcgaa
     9781 tgagacaaag aagatcctcg atcgattatt tcctctgaga aaatgcaaac ctagtcagac
     9841 gaaggagcct tgtctttact atcatttggg acaatgcctg tgtccttatt atttcgacgt
     9901 agatccgaaa gtctatacga aaatcgttga tgaagtgaaa aactttttaa acggaggaca
     9961 cgaaaaaatc gagtcagaga tccatcagaa aatggaaaaa gctgctgaaa acatggagtt
    10021 tgaaaaagct gcagaatacc gagaccagat ccgtgccatt gagacaatca tgacttgcca
    10081 aaaaatgaca aatactgatt tactggatcg agatgtattt ggttatgcga ttgataaagg
    10141 atggatgtgt gtccaagtgt ttttcgtaag acaaggaaag ctaattgaac gagatgtttc
    10201 aatgtttcct ttctataacg aagcagaaga agatttcttg acttacctcg gtcagtttta
    10261 tcaggaaaat gagcatttca ttcctaaaga agtcttgatt cctgatacga tcgacaagcc
    10321 cagtgtagaa gctttgttag ctacgaaagt gctgcaacca aaacgaggag aaaagaaaaa
    10381 attagttgaa ttggcgagta agaatgcctc agttgctttg aatgaacggt ttgatctgat
    10441 tgccagaaaa caggaacgga cgatcggagc agttgaacgt ttaggaaacg cgatgaatat
    10501 cccgacccca atacgtatcg agtcttttga taactctaat atcatgggga cgaatcctgt
    10561 ggctgcgatg gtcgtattca ttgatggaaa accggcaaga aaagaatatc ggaaatataa
    10621 aataaaaaca gtcaacggac cggatgatta tgcatcaatg agagaagtca tctatcgcag
    10681 atattcgcga gtgattcgcg aagggttgcc tttacctgat ttgatcttga tcgatggagg
    10741 aaaaggacaa gtagatgtgg caaaagatgt cttagaaaat caattaggaa tcgatattcc
    10801 tattgctggc atggtaaaaa acgataaaca taaaacaaat gagttgctat tcggtacaga
    10861 tttggctgta gtaccgcttg agcgaaattc accagagttt ttcttactcc agcgaatcca
    10921 agatgaagtc catcgattcg caatcacttt ccatcggcag ctacgcagta aaaacagttt
    10981 tgcttcccga ttagatgaaa ttgaagggtt aggaccaaaa cgaaaaaaaa tgctgcttaa
    11041 agagttcaaa tcgttgaaaa atatcacagc agccacgaca gaagaattac aagaaatcgg
    11101 actgccgaaa aatgtcgcac aaaacgtatt tgataaatta catcaggaat gaggaaaggg
    11161 cactttacaa agaaagtaaa gtgtcctatc attttatgta caacaaaaag agcgtttacg
    11221 ttttgttggt aaataataaa tcgcttacgt ctggtgtcta aggatatatg tgatttcttg
    11281 aagaaggagt gaagaaatgt tagaagtcaa ggatcttgtt aaaacttttg ggaactacac
    11341 agctgtcgat catgtctctt tccaaatccc cgatggaaaa atcatggggc taattggcca
    11401 aaacggggct gggaagacaa cgacttttcg tttgatcttg gattttttga cacaagacca
    11461 aggagaagtg ttatggaatg gccatcatct tggggagaaa gattatgatg tcatcggtta
    11521 tcttccggag gaacggggac tttatccgaa agtaacgatt caagaccaac tgctttattt
    11581 tgcagaactt agagggaaat caagaaaaga gattgaacca aaaatcgact tttggatgga
    11641 aaaatttcag gtaaaaggga aaaagacgga taaagtaaaa tctttgtcta aaggaaatca
    11701 gcaaaaagtc cagctgatcg ccacgttaat ccatgaacct aaattgatca ttttggatga
    11761 gccattcagc gggttagatc cggtaaatgc tgaattgcta aaagatggaa tattggaatt
    11821 gaaaaaacat ggttcatgtg tcattttttc tagccacaat atggataatg tagaaaaaat
    11881 ctgcgatcat ttgatcatgt tgcgaaacgg aaaaatggtt ttgaatggaa aagtacatga
    11941 gattcgtgaa tcatttggcc gtacgaagtt attcttggaa tctggtttaa gccaacagga
    12001 agtagaagaa atcgaaggcg tgaaaaaagt gatccttcat gaagacaata cgttagagat
    12061 taccttagaa gatcctgaag ctgggaaaga aatctttact cgtgcgactc agttcggcta
    12121 cattcctatg tttaaccagc aaccgccaac tttggaagag attttcaaat tgaaagcagg
    12181 tgaaatcgat gcgtaagttt tggattatta ccaaagatgt ctatttaaaa aatgttaagt
    12241 cgatttcttt cttgatcatg atattagtcc cttttatcat gctgggtatc atttatgtag
    12301 caggaaactt tgctcaacaa aatagtgatg tcaataaaat cggcgtattg gctgataatg
    12361 agcaaatcag tcagcaaatg agccaagtca aaacagatga ttttgagttc aaagcagttg
    12421 attctgaaga aaaagcacaa aaagagcttt cggaagaaaa aatcgatgcg tatttagtag
    12481 tctcaactga gaatgataag ataaatggga tgctttatag cgaaaattca ttaggacaaa
    12541 gtacacaact actgatacaa caacaactga ctggcttgca atcgatgctt cgagcgagtc
    12601 aattaggcat ttcaccagaa gaagtagcta gcttgagcca acaagcaagt ttgactcgac
    12661 aaaaagtcaa ttttgattca aaaggaaaaa tgacagtcgg agaagataac tcgacagtcc
    12721 aatacgtcgt aagttatgta gcaacgattg tcttgttcat tattattttg acgtatgcgc
    12781 agatcattgc acaagagatc gcttctgaaa aaggcacgcg gatcatggaa gtgatcctgt
    12841 ccagtacgac tgcacaaaag catttttacg gaaaattaac aggtgtttta cttgttgcat
    12901 taacgcaaat ggcattatac ggtattattt ttggtatagg atacagtcaa ttcaaagata
    12961 tggaaatggt caaaaatata ttagacggta tctcattgga taacttgatt ggaccattct
    13021 tgtggtattc tcttctattc atgatctttg gcattttgat tttttctgtt ttagcagctt
    13081 tatgtggctc tttggtcaac aaagcagaag atacagcaaa agcaattttg ccagtgactt
    13141 atctatctct aggcggatac atgcttggat tgatactagg agcttcagat ccaaacaata
    13201 tcgttattcg gatcacttcg tatattccat tcttatcttc atatattatg ccgattcgct
    13261 tagcaaatga gacagtcggt atgggtggag cattgatttc cttgttggta cttgtagccg
    13321 taacattctt attgatgatc ttgtctgcaa acatgtacaa atcgaatgta ctcgtttata
    13381 gcgaaggcgg attatggagt tcattgaaac aatcgatttc aattatgagg aatgaacgaa
    13441 aaaaagcata aaacaatcct aaaatacaga aggaagctgg acatctattg ttcccagctt
    13501 ctttttgtat agacctattt tatttatttt actatattgg atatatattc attgaaaata
    13561 gtgtataaca actttataat attgagtata ccaatttttg gaggcgtttt aatggaccgt
    13621 tatttagatt tactttcaga aaaattcccc tcaacggagg atgtcatcac ggaaatcatc
    13681 aatctcgaag caattttgca gcttccaaaa ggaacagagt tatttttgag tgatatacat
    13741 ggcgaatttc ctgcttttga tcatattctg cgtatcggtt caggaaatct taaagaaaag
    13801 atcaaagacc ttttttctga acaaatgacg gaaacggata tcagccaatt cactattttt
    13861 acggcttatc cagaatatgc attaacgact gattggtaca aagaacaaga taaatcgcag
    13921 ctgatccatc atttgatcga tcttttacgg tttacgacag tcaaatatac ccggtcaaaa
    13981 gttcgaaaag gattgccgaa agaatatagc tatatcattg aggaactctt gtatatcgat
    14041 gaccgtatca atggaaaaaa agactatgta aaaaaaatca ttgaacaact tgttttgatg
    14101 aaggaagaag aaaaattttt aacgaaactt gcacagacga tccaaaaatc tgtcatcgat
    14161 catttgcata tcgttggaga tatttttgac cgaggaactc aagcagccaa agtgatggat
    14221 cgaatcatgg atttttcttc tgtagatatc caatggggaa atcatgatat tttgtggatt
    14281 ggtgcgtatt gcggctctga ggcatgttta ctgacacttt tacggattgc agcacgctat
    14341 aattatttat ttgaattaga gaaagaatat gggttgaatt tacgtccatt gttttctttt
    14401 gctcatcaaa cttatcagaa gaatccagta tttgcgccta aaaacagtaa aaatctatcc
    14461 gaaagagagc aagaagaatt agaaaaaatc catcaggcac tttctatcct tcaattcaaa
    14521 tcagaacatc agttattgga tcgtcgacct gaatttaaaa tggacgatcg taaattgctg
    14581 gaaaaaatcg actatgaagc atcaacgatt gatttagaag gtcagctcta tgagttgaaa
    14641 ggaacttgtt ttcaaacgat ccagccagat gatccaaaaa aattcttgga agaggaacaa
    14701 aaagtgattg atagtttgat gtattcattc cagcactctt tgagaatgca aaaacatatg
    14761 acgtttttga tcgaaaaagg gggaatgtat ctcacgaaca atcagcatgt gctgtttcat
    14821 ggctgtatac ctgtagacga gaagggacag ctcttagcat ttgaattaga ccaatcttat
    14881 cgcggaaaag aactgctggg cttttttgaa aaacagataa ctgaaagcgc aaaagatctg
    14941 actgcaaaag aagatttagc aaccgatttg atttggtatg cgtggagtgg gccattttcg
    15001 cctttattcg gcaagagtaa aatggcgact tttgaacgtt attttctcac agagcctcat
    15061 acgcacgtcg agaaaagcaa tccttattat cacttgcgag atgaagagtg gttcagtgaa
    15121 aaaattttag cagaattcga cacaaaagaa gaagggtcag caatcattaa tgggcacacg
    15181 ccagtaaaag taaaaaaagg ggagtcaccg atcaaagcaa atggaaaagt atttgtcatt
    15241 gacggaggtt tatccaaagc ttatcaaaaa acgacaggaa ttgccggtta ttcactatta
    15301 tgcaattctt atggtttcca aatcgtgaca cattatccgt ttttgcctat tgaacaacaa
    15361 tttgcaaaaa aatccgacca aacaaatgtg aaaaaagtaa tcgaacagca actaccacgc
    15421 aaactcaatc gtgatacaac aaaaggtatg gaactgcagc agcagattac ccagctcaaa
    15481 aggctgttgg actatcgaaa ggacgattga gaagaattcc agtgaacatt gttccataga
    15541 ctggagactg agacaaaagt cgtgtcgcag tctctttttc cggataaacg gtgttcttct
    15601 gccaactcct cggtcacaag tcacactatt caacaaacat gaaaagctgt tttttgaaat
    15661 tgctccttgt gactcagagt tgaacggcag gctcacgacc tcttggtata aacggtgttc
    15721 aaaagccagt ttctcggtat taagccaaat ttatgcaaaa atgtgaaaag ccattttcac
    15781 aaaatttttc ttaatactcg aaactaaaca gctttttcac aaccccctca gaaagtctat
    15841 taaaattcat tcacaaagaa atcgaataag cgctgttcag atggatgtac acggtgggta
    15901 agttccggat gccattgaac accgaggatt ttatgcatat catctttgct ttgaaccgct
    15961 tcaacgagtc catcgttaga aaaagcgatg gcttttaata aaggtgaaag atctttgact
    16021 gcctgatgat ggtaagaatt cacaaaatat ggatcgggga gaaggttgct taacaaactg
    16081 tctgaaacaa tcttgacttc gtgagtcgta aattgcgggg cagtgggctg ttgttcatgt
    16141 tttactgacc actttggata taatgaaaga tcttgataaa gtgtaccttc caaaacgaca
    16201 ttcaatagtt ggagtccccg gcagactgca aaaattggtt ttctttgttt caatgcttcc
    16261 aagatcagtg cctgttcaaa ttggtctcgt tgaatatttg tttccgttag ctttggatga
    16321 ggatcttcca tgtaaagttg tggactgaca tcttgaccac ctgcaagaat aagtttgtcg
    16381 atttgtccaa tcagttgagg agctgaatct ggagacatga ctggcagaat aagaggcaat
    16441 ccttgagcat ctaaaactgc atccacgaat ccttgcggtg tatagctcac ttgatttcct
    16501 tggaaagtat ctgttgctcg aatcagttga tttccggcaa tgccgatgat tggtttcatt
    16561 tgtattcccc ctaaagtaga cttattgtaa caaggattta aaaaaaatta aactatgtaa
    16621 gacttgcccc tatacttatc tgttcttgct gtgttactat ctgtaaagag aaatttcgtg
    16681 tgaaattcat gacgatagtt gaaaatattg cttaatttgt tataataaaa agtaagcaga
    16741 aataaaaagg ttgggcgtaa gcttcaagga tctagttctt caaggggtga gaaggacatt
    16801 ttattgaagg ggcctgccta tactaagtta tctttagagg tacatacttt caatgaaaaa
    16861 agaaagcatg tacctctttt ttaaatcgaa aggaagtgaa gcaagtgtta gaagctttgg
    16921 aacaaatccg aaaagcagaa gaagaaaatg aaaagcgtgc tcaaacactg cggcagcagc
    16981 tgaagatcta tgcaacagaa aaagagcagg aattggaaac gatccaaaca aactatcgac
    17041 aacaactaaa acagttggtg gaagaaaaag aacaatttga aatacaagaa ttagaaaaag
    17101 aaaaaatgaa attgaaagca gaagcagaca aggtgcaagc aactttgaaa gatcaatatc
    17161 aaaagcatca gagtcaagcg atcggtgcga tcattgaaag ggtgaaagaa acttatggcc
    17221 gtcactaaga tggaaaaagt gaccctcatt tcagataaaa gaaatcagga gaggatttta
    17281 caagcagtac aaggaatcca aaacgttgaa atccgggatc tgtatcaaaa tagaacaaac
    17341 aaccagtggg tggagaagta ttttccagat accacgatta ttgataaaga agcaaaatta
    17401 agcgctttag gtaatcggtt gaaagacatc caagaagcta tccaattcat acaacaccac
    17461 ggcgatagcc gtcaaaagaa actccatttg aaacgtcaag aactttcctt acaagatttg
    17521 gaagccaatt attccgaaga aaatttcata aataaattga aagaagtcct tgatttaaaa
    17581 gaaaagtggg aacaactaac tgaagaacgt gatcgtttag aagaagagga agaatggctt
    17641 cttcattggc aacagctgga tattgctcca agtatgtatg caagtaagac gaccattttt
    17701 gagatgggga ctgtgagtgc agcaaatttt gaaagtttca aggaagaact gagtaagctt
    17761 gatgacgttt atatggaaga aatagattat acgccaaaac aagtttatct ttcgtatgtt
    17821 gctttaaaaa aatcagttgc tgttatagaa gaaattgctg ggaaatacgg gttcaataaa
    17881 gaaacgtatc cttatgaaca aaatcctaaa gaaaaactgc aagagaccaa gcagcaatta
    17941 tccattgttc acgagcagca aaagaagata acgacagcgt taggtcagtg cagtggctat
    18001 atccaagaat ttgaatgggt agaagaagtc acacttgccg ttgcagaacg agaaaaaata
    18061 aaagaacggt ttatccatgc gacttatctg atcgttttgc aaggttgggt agatgcagaa
    18121 gaaaaacaga atttgctgca tgtgttaaac gaaaccgtat cagaagaaga tatatacgtc
    18181 agtttcgaaa caccaaactc agaagagatt cagcaggaag tccctactaa attgaaaaac
    18241 catccgcttg tcgaaccttt tgaattgctg actgaaatgt acagtctgcc aaaatatgaa
    18301 gaagtagatc caacaccttg gatgacacca ttttatcttg tcttcttcgg gatgatggtc
    18361 gcagatgttg gttatggttt gctgatgctc attgggacaa tactagctca aaaactactt
    18421 gttctaccac gcgggatgaa acgtttcgta aaattctttg agattctctc gataccatcg
    18481 atcatctggg gattgatcta cagttcattt ttcggacagt cattaccaaa agaactgttt
    18541 ggcatccgct tgcctttccc aatcctatct acgacggatg acgtcaatac gattttgatt
    18601 ttatccgtga ttttcggtct gatacagatt ttggttggat tatttgtggc agcaaaggaa
    18661 aatatcagga gaaaagatta cttgagtgct gtcagtgatg gatttgcctg gcaagggatc
    18721 ctaattggga tcgttattgc gttggttggt gccatgttga taaaaaatac agcatttgtt
    18781 taccttggcg ctgggctatc gatcattgct gccttatgta tcttgatcgt tccgattatc
    18841 caaacgccgt ctaaagtcaa aggtgctgcc aaaggtgcgt ataatcttta cggactgacc
    18901 ggctatatcg gtgatttagt cagttataca cgtttgatgg cattgggtat ttcaggcgga
    18961 agtattggag cggcattcaa tatgctagtt gctttcatgc cgccagctgc tcgattcagt
    19021 gtagggatcc ttttgatcat cgctttacac gcactgaata tgtttttgac actgctaagt
    19081 gcctatgtcc atggcgcacg attgcagtat gtcgaatttt ttgggaagtt ttatacagga
    19141 ggcggacgtg cctttgatcc gctgaaaacg gcggaaaaat acgtcaacat caatcacaag
    19201 aaaaaaaaca ataaataatt ggaggatttt tatataatga tggattactt aattagtcaa
    19261 aatggtggaa tggtatttgc ggtattagcg atggcaacag cgacgatttt ttcagggatc
    19321 ggttcagcta aaggtgttgg aatgacaggt gaggcagcag cagcattgac aacaagtcaa
    19381 cccgaaaaat tcgggcaggc tctgattctg caattgcttc caggcacgca aggtttgtat
    19441 ggattcgtta ttgccttttt gatttttatc aacttaggaa atgacatgtc agttgtacaa
    19501 ggactgaact ttctaggtgc ttctctgccg attgctttta caggcttgtt ttcaggtatt
    19561 gcccaaggaa aagtcgcagc ggcaggtatt caaattttag cgaaaaaacc tgaacatgct
    19621 acaaaaggaa tcatttttgc tgcgatggtt gaaacatacg cgattttagg tttcgttatt
    19681 tccttcctat tagtgttgaa tgcctaagca taaaggagga aaagcagatg aacgccattg
    19741 aaaaaatcat ctctcagatg aacgaagcag cagagcagga aagagcggcg ttagaacaag
    19801 aagagcgaat gaagatcgat caaaactttg aacaaaaacg tacgcaagtt gaaacagaac
    19861 atcaaaaaca aaaagaaaaa caaatcgaat tactcgaaaa aaaataccgt cagctaagga
    19921 atcgccagca agtagaagta cgacaagaaa atttgaatgc gaagcaggaa tttttaagac
    19981 gattgtttgc agatgcggta actgaaatgg aaaattggga tgaatctgaa caaatccagt
    20041 ttatcaaaaa tgcattgtat tcattacctt tgacaggaaa agtagcattc attgctggag
    20101 aaaaatcagc agcctatctt tctcagacgt tgttggatga atggaacaat gaacttccct
    20161 ttatgatggt tttaagtgat gaaactgttg ctgatcaagc gggttttctg ataaacgatc
    20221 aaggagttca gtataatttt cttttcagca gccttgtaca agatatccaa ggaacgatga
    20281 gttttgagat tgccaatcaa ttattcgaat aaggagggat cagtgtatgg aataccacga
    20341 actaaatccc ttgattcgcg gaagagagct tgaacttttg tccaatgaga catttgaacg
    20401 gatgattcaa acaaattctg tcgaagcact tggtgaaata ttgaaatcca cgatttacag
    20461 tccatatatt tatgacggat atgaatatga cttcgaacag aatcttttaa aggagcagag
    20521 ccgtctattt cgctggttaa aggaatcagc accggagcct gaaatcgttt ggatctatac
    20581 gatgcgttat actttccata atctgaaggt tttgacaaaa gctgagatga caaatcaaaa
    20641 tctggaccat ttgtatatcg acgatggatt ttattcatta gaaaccttga aggatgccat
    20701 tcatacgcaa gtttcaaatg aacttcctga aagcattatg gacagtatac gagaagtacg
    20761 tgattatttt gaagagtcta ccattcttca gggaattgac gtgatttatg atcgacagtt
    20821 tttgacagag cagcgacggc ttgccgataa gctaggatat caagaattgc tgacagaagt
    20881 cattgctttt attgacttga ccaatatcac aacgatggct cgaggaatat tgcagaaaag
    20941 aacatatggg ttcatgacga cggtactttc cagctcggga agtatcgaaa aagaagtatt
    21001 tttgaacttt gtagataaag atatggaaga ttacacgaat tttctattag agacagatta
    21061 tgctgatttt ctgcgccctg tcatccgtga gggaaatatt gatctcgtaa agcttgaaca
    21121 gttgaaagat gattatctaa gttcattgta tcaagaagca caaacccagg ctttcggacc
    21181 actgccacta ctagcctttt tgaacgcaaa agaggtagaa agcaaaaacc ttcgtttact
    21241 ggttatcggc aaaagaagcc atttcgaatc agaaacaatc agaaagcggg tgagacaagt
    21301 ctatgacgca taagatcggt gtagttggcg acaaagattc tgttttgcca ttcaaattgt
    21361 ttggctttga tgttcgttat ggtacaacca agcaaaccgt tcgtcaggca attgaagaaa
    21421 tggcaaagaa tgagtatggc gtgatttatg tcacagagca atgtgcagca ttgagcccag
    21481 aaacgatcga acgttacaag gaacaaatga tacctgctat cgtattgatt cccaatcatc
    21541 aaggatcatt agggataggg ctggaagaaa ttcaaaaaaa cgtagaaaaa gcagtaggtc
    21601 aaaatatctt atagtatgaa aaggagatag aaattttgca aattggaaaa atcataaaag
    21661 tctccggacc tcttgtaatg gctgagaaca tgtctgatgc cagtatccaa gacatgtgtc
    21721 tagtgggaga tctaggagtt atcggagaaa ttatcgagat gcgtggcgat gtggcgtcta
    21781 ttcaagtata tgaagagact tctggaattg gtccaggcga accggttcgc tctactggtg
    21841 aagctttatc tgttgaatta ggaccaggga tcatttcaca aatgtttgac ggaatccaac
    21901 ggccattaga tacctttatg gaaatcacac aaagtaattt cttagggcgc ggagttcaat
    21961 tgcccgcatt ggaccatgag aaaaaatggt ggttcgaacc aacagtagaa gccggagaaa
    22021 cggtgagtgc tggagatgtt atcgggatcg tagaagaaac aaaagttata aaacataaaa
    22081 tcatggtgcc aaatgggata aaaggaacga tcaaaacaat cgaaaaaggt tcattcacta
    22141 ttgatgaaac agtatgtgtc attgaaaccg aaaatggtga caaaaatttg acgatgcttc
    22201 aaaaatggcc agtaagacgt agtcggccaa tcaaagaaaa attaaacccg gatgcaccca
    22261 tgatcactgg acagcgagtg attgatacat tcttcccagt gacaaaagga ggagcagcag
    22321 ctgtacctgg tccttttggt gcagggaaaa cagtcgttca gcatcaaatt gcaaaatggg
    22381 ccgatgtgga catggttgtc tatgtgggat gtggcgagcg tggaaatgag atgacggatg
    22441 ttttaaatga attccctgaa ttgattgacc ctaatacagg agaatcgttg atggaacgta
    22501 cggtattgat tgctaataca tcaaacatgc cagtagcagc tcgagaagct tctatctata
    22561 cgggaatcac aattgcggaa tatttccgtg acatgggtta tgacgttgct atcatggcag
    22621 actccacttc acgatgggca gaagcacttc gtgagatgag cggacgtttg gaagaaatgc
    22681 ctggtgatga aggttatcca gcttatcttg gttcacggct tgcggaatac tatgaacgat
    22741 ctggaagagt cattgcatta ggatcagaag gtcgcgaagg aagtataacc gcaatcagtg
    22801 ctgtatcccc gtctggaggg gatatttcgg aaccagtcac acaaaataca ttgcgggtcg
    22861 taaaagtatt ctggggtctt gattccagtt tagcccaaaa aagacatttt ccttcgatcg
    22921 attggataca aagctattct ctttattcaa cagaagtagg gaagtatatg gatcatatcc
    22981 ttcaagctga ctgggcatcc atggtcaccg agggcatgcg tattttgcag gaagaacagc
    23041 agctgaatga aatcgtgcgt ttggtaggga ttgattccct ttcagataat gaccgtctga
    23101 cattagaagt ggcaaaatcc attcgtgaag attatttgca gcaaaatgct tttgatgatg
    23161 tcgatacatt cacttcaaga gagaaacaat tcaaaatgtt gaagatgatc ttgacctttg
    23221 gtgaagaagg acgccgtgcg ttgtcgttag gtgcttattt aagtgagatc atggacggta
    23281 ccgtatcgat tcgtgaaagg atcggacgaa gcaaatatat cccggaaaca gaattagaaa
    23341 aactagatca aattattggt gaaatcaagc aaacgatgca aacgatcgtt tcagaaggag
    23401 gcatgagcaa tgattaaaga atatcgtacg atcggtgaag tcgtcggtcc attgatggcg
    23461 gttgaaaaag ttgctggtgt caaatatgaa gaattaatcg aagtccgtat gcaaaatggc
    23521 gagattcgtc gtggacaagt attggaagtc caagaagata aagcaatggt ccaaatattc
    23581 gaaggaacaa gtggtatcaa tttacgtgac tcttctgtac gatttttagg ccatccttta
    23641 gaactaggtg tatcggaaga catgatcggt cgagtattcg atggtttggg acgaccgaaa
    23701 gataacggac cagagatcct gcctgaaaag catttggata ttaacggaga agtaatcaat
    23761 ccagtagctc gggattatcc agatgagttt atccaaacag gtatctctgc aatcgatcat
    23821 ttgaatacat tagttcgtgg gcaaaaatta ccagtatttt ctggctctgg tcttcctcat
    23881 aaagaattag ctgctcagat cgcgcgacaa gcaacggtac tcgattcatc agatgatttc
    23941 gccgtagtct ttgctgctat tggaatcact tttgaagaag ctgaattttt catggaagat
    24001 tttcgtcaaa cgggcgcaat cgaccgttct gtcatgttta tgaatttagc aaacgaccct
    24061 gcaatcgaac gtatcgctac tcctcgtatg gctttgactg ctgcagaata tcttgcttat
    24121 gaaaagggaa tgcatgtttt ggttatcatg acagacatga ccaactatgc agaggcattg
    24181 cgagagattt ctgcagcacg gcgagaagtt ccaggaagaa gaggatatcc tgggtatctt
    24241 tacacgaact tagctacttt attcgaacga gcaggaagaa tccgcggact aaaaggatcg
    24301 gttactcaaa ttccgattct aacaatgcca gaagacgata aaactcatcc aatccctgac
    24361 ttgactggtt atattacaga aggacagatc atcttgacta gagagcttta caagagtggg
    24421 attcagccac caattgatgt actcccgtct ctttcgcggt taaaagataa gggaactgga
    24481 cccggaaaga caagagaaga ccatgcagct acgatgaatc agctttttgc agcctatgca
    24541 caaggaaaac aggcaaaaga attagccgta gttttaggtg aatctgcttt atctgatgta
    24601 gacaagatct atgcgaagtt tgctgagcgg tttgaaaaag aatatgtcaa tcaaggattc
    24661 tacacgaacc gttcgattac tgaaacactt gatctagggt gggaactgct atcgatgttg
    24721 ccaagaaccg aattaaaacg aatcaaagac gacatgctgg acaaatatct gccagaaggg
    24781 aagtgagttc tgatggcacg attgaatgtg aatccgaccc gcatggaatt gactcgattg
    24841 aaaaaacagt tgacaactgc cacaagaggg cataaattat taaaagataa gcaagatgaa
    24901 ctgatgcgtc aattcatcct gttgattcga aagaacaacc agctgcgtca agaaatggaa
    24961 gagtctatga cagaagcaat gtctgatttt gtattggcaa atgcttcgat cacggaacct
    25021 ttcatcgaag aattatttgt tttgccggcg gaaaacgttg atttgtctat cgtagacaaa
    25081 aatatcatga gtgtaaaagt acctgtgatg aattttgaat atgatgaaca gctgacagac
    25141 acacctttgg aatacgggta tttgaattcc agtgccgaac tggatcggtc aatcgatcgg
    25201 tttactaccc tgcttccaaa acttttggaa ctgacagaaa tcgaaaaaac ttgtcaactg
    25261 atggcgagtg aaatcgaaaa gacacgtcgg cgagtcaatg cattagaata tatgacgatc
    25321 cctcaattag aagaaacgat ctattatatt cgtatgaagc tggaagaaaa cgagcgcgca
    25381 gaagtaacaa ggttgatcaa agtaaaaaat atgggtacca ctgactaagt ggtatctttt
    25441 tttaaaagag gaaaacgatg attaaacaaa gcaaacaaat aaagaaacgt ttgccggctg
    25501 ttcagcttct cgcattagga ttttttctcc tgattttagt tggcggcggc ctacttaccc
    25561 taccattttt tagtaatagt gggcatggga caaattttat cgatgcgctt ttcactgcaa
    25621 cttctgctgt ctgtgtaacg ggattgacaa cattgaatac agcagagcat tggaacagtg
    25681 ctggtcaatt tttgatcatg cttttgattg agatcggcgg tcttggtttt atgatggttc
    25741 cgattctatg ctttgcattg ataaagaaga aagtcagtct cagcacacgt attatattaa
    25801 aagaagcgct gaatctagaa gaagtatcag gtgtgatgaa gctcatgctt tacatcctaa
    25861 aattggcctt tgtgatccaa ttgattggta cggttgcttt atcttttgta tttgtgcctg
    25921 aatttggctg gttgaaaggt atctggtaca gtttgttcca tgcaatctct agtttttgta
    25981 atgcagggtt tgatttgtta ggagacagtt tagccaatca ccagcaaaat gtttatttga
    26041 tcatggtggt atctgctttg atcatttctg gtgggttagg cttcatcgta tggcgtgatt
    26101 tactggaata tcatcagaaa aagaagatga cgattcattc aaaaatcgca ttaaccgtta
    26161 caggtaccct tttattaggc ggattcgttg tgttcttttt cacagagcgc aatggggcaa
    26221 cgctagtgga agggaatatg tttcaacgat tggcaaacac cttttttatg agtgtgacac
    26281 ctagaacggc tggatattat tcagttgatt acctccatat gagccatgca ggtctgattt
    26341 taacgatgtt tttgatgtac atcggcggaa catctgggtc gacggccggc ggattgaaaa
    26401 caactacgtt aggtgtgttg ctgatccaga tgcgttcgat gttgaaagga cgtacgagag
    26461 cggaagcatt tggccgtacc atccgacaag gcgcagtgtt gcgggcactt actttatttt
    26521 ttgttacgtt gacgttgtgt gtagtcgcca tcatgatttt atcggtgact gaaacgatac
    26581 ctaaaacttc tggcatcgaa tatattgctt ttgaggtctt ctctgcgttt ggtacagtag
    26641 gtcttacgat gggattgacg ccagacttga ctgcaatagg gaaattgatc attatagcgc
    26701 ttatgtacat tggacgagtt ggtgtattga ctgtggtctt ctctctgatg acaaaagcac
    26761 aaagaacaca atcaaaatac aaatatccag aagaaagtgt catgattgga taatttcatc
    26821 ttctgacaga aacctagtga tttagtaaaa taagaaaata aaataatttt tcttgcttct
    26881 tggatacttt aagtgcaaaa tgttcgttta ttttttgagg cactacccgt tgttgaacct
    26941 cgggtagtgc ctctttttta ttgaaaggaa tcaataagat ttgattcctt
//
LOCUS       DAJSKS010000022        26840 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJSKS010000022 DAJSKS010000000
VERSION     DAJSKS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.022"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            80..1366
                     /gene="efmA"
                     /locus_tag="ON396_000827"
     CDS             80..1366
                     /gene="efmA"
                     /locus_tag="ON396_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HCR3112954.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1504..2103)
                     /locus_tag="ON396_000828"
     CDS             complement(1504..2103)
                     /locus_tag="ON396_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HCR3112955.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2507..3628
                     /gene="mnmA"
                     /locus_tag="ON396_000829"
     CDS             2507..3628
                     /gene="mnmA"
                     /locus_tag="ON396_000829"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR3112956.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            3874..5295
                     /gene="arcD"
                     /locus_tag="ON396_000830"
     CDS             3874..5295
                     /gene="arcD"
                     /locus_tag="ON396_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCR3112957.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5309..6427
                     /locus_tag="ON396_000831"
     CDS             5309..6427
                     /locus_tag="ON396_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HCR3112958.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6487..7779)
                     /locus_tag="ON396_000832"
     CDS             complement(6487..7779)
                     /locus_tag="ON396_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR3112959.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            7911..8489
                     /locus_tag="ON396_000833"
     CDS             7911..8489
                     /locus_tag="ON396_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3112960.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8618..8995
                     /locus_tag="ON396_000834"
     CDS             8618..8995
                     /locus_tag="ON396_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCR3112961.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            8928..11000
                     /locus_tag="ON396_000835"
     CDS             8928..11000
                     /locus_tag="ON396_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR3112962.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(11211..12158)
                     /locus_tag="ON396_000836"
     CDS             complement(11211..12158)
                     /locus_tag="ON396_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3112963.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            12404..12868
                     /locus_tag="ON396_000837"
     CDS             12404..12868
                     /locus_tag="ON396_000837"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR3112964.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(12930..13973)
                     /locus_tag="ON396_000838"
     CDS             complement(12930..13973)
                     /locus_tag="ON396_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HCR3112965.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(14350..15315)
                     /locus_tag="ON396_000839"
     CDS             complement(14350..15315)
                     /locus_tag="ON396_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCR3112966.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            15549..16394
                     /locus_tag="ON396_000840"
     CDS             15549..16394
                     /locus_tag="ON396_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3112967.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            16585..17772
                     /locus_tag="ON396_000841"
     CDS             16585..17772
                     /locus_tag="ON396_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR3112968.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            17778..18134
                     /locus_tag="ON396_000842"
     CDS             17778..18134
                     /locus_tag="ON396_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCR3112969.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            18136..18474
                     /locus_tag="ON396_000843"
     CDS             18136..18474
                     /locus_tag="ON396_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCR3112970.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    18550..18806
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            18869..19903
                     /locus_tag="ON396_000844"
     CDS             18869..19903
                     /locus_tag="ON396_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3112971.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            19896..20582
                     /locus_tag="ON396_000845"
     CDS             19896..20582
                     /locus_tag="ON396_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3112972.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            20606..21454
                     /locus_tag="ON396_000846"
     CDS             20606..21454
                     /locus_tag="ON396_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR3112973.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            21651..22424
                     /gene="sufC"
                     /locus_tag="ON396_000847"
     CDS             21651..22424
                     /gene="sufC"
                     /locus_tag="ON396_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR3112974.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            22437..23723
                     /gene="sufD"
                     /locus_tag="ON396_000848"
     CDS             22437..23723
                     /gene="sufD"
                     /locus_tag="ON396_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR3112975.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            23720..24955
                     /locus_tag="ON396_000849"
     CDS             23720..24955
                     /locus_tag="ON396_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR3112976.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            24942..25412
                     /locus_tag="ON396_000850"
     CDS             24942..25412
                     /locus_tag="ON396_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCR3112977.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            25417..26808
                     /gene="sufB"
                     /locus_tag="ON396_000851"
     CDS             25417..26808
                     /gene="sufB"
                     /locus_tag="ON396_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR3112978.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
BASE COUNT         8780 a         4975 c         5791 g         7294 t
ORIGIN      
        1 gtattttttg aaaaaagaat cccgtataat gattgttgtg caaacgctat acaaaatgaa
       61 aaagaaaaga gggatgttta tggaaaatga acagtcggtc gttttaacga attggaagag
      121 aaattatttg ttctttttat cgggacagtt tttgtcagga atcactagta tggtcgtcca
      181 atatgcgatc atctggtatt tgacaagaga aaccggttcg gcgacaattt tgagttttgc
      241 tactttacta ggaatgattc ccatggtttt actaagccca tttgtaggac cgctagtgga
      301 tcgctgggat aaaaaagctc ttttgattgt cacggatatt attgtagcga ttttcgcgct
      361 tattttagca gtagtcggaa cgatttcaga atccttcccg atttggctag tttttgtgtc
      421 tttgtttatg cgttcggtcg cacaaacatt ccaaatgcca acgatccagt cgattatgcc
      481 gacaatcgtt ccatcttctc acataacaag gacgaatgga cagttaggaa tggtccaatc
      541 agcgaatttc atcattgcgc cagctctagg tgcggctttg ttttcagtcg ttcctgtcaa
      601 ttaccttatt ttattagatg tattgggggc agtttttggt gtcggcttat tgatttttgt
      661 gaagatccca aaagtttctc ctgaaatatt ggaagttccg cttactattt ttaaagatgc
      721 aaaatttggg ctgcagcaat tgatggataa taaagggtta tggtacataa cgatcaatgg
      781 tgcatttgtg atgctgttgt tcatgccagc aatcagtctg tatccattga tgacgctaga
      841 ttactttggt ggttccgttg gacaggcggg agcagtggaa gtagtctacg cagtcggaat
      901 gctcttaggc ggtgcactga tcagttttat aggaacatgg aaagaccgaa tgaaacctat
      961 tattatagcc tacatcatta tgggcctgac gatcggtgca agcggattgg ttccaaacga
     1021 tagtcaaggg ttcttgtact ttcttatctt gaatgcagga gcaggttgtg caacgccata
     1081 tttcaataca ctgctgatgg caatgatcca gcagagttat gaatcgaatg ttttaggtcg
     1141 tgtcttaggg aacttcaact ctttgatgaa cctggcaggt ccaattggat tattatttgc
     1201 aggaccatta gctgatcgtt taggggtaga aaaaatgttc ttgttttccg gaatcgggat
     1261 tttattatgc ggaatcgttt tatttttaac gtcagctgcc cgaaattatg ataaagaatt
     1321 acagaaaaaa ctggtaaaag aacaccatga gcaaaaagac gaatagtatg gaatggagtt
     1381 gtgacagaag tcttgttaca actccatttc tgatcctaat gttcctgtgg caataagcct
     1441 actcaaatga aaaatatgag taattatctt tatttgttta atacttgata tagggcagtt
     1501 ttttcacgac ctatcgtact tcctctttgc ttcatagatt tctttgattt gttgctcaga
     1561 taacggagtg ccaaatggta gtttttcctt taagagttct ctttccggtg cgtctacgcc
     1621 tacattgaca tcgttctcga taggtacgct gtaatgatgg atatgtccat gaagattgcg
     1681 gatagtgtcg ttaggcccga gcaacatcgg ataatgagtc aagtaatatt gttgatggtc
     1741 gaatttaacg atggcaccta catcataaaa ataaaatttg ggacttccgt cagtaagcct
     1801 aggatcattt ttttctaaat aacggaaaaa agcgcgactg tcatggttcc ctttgatcaa
     1861 gtggattgtt ccttgcagtt gagtgaccag ttccaaaatt tctgcatttg ctttttcata
     1921 ttgcggatgc atagcaaggt cgcctagatg ataaacatgg tcggtttctt ttattactgc
     1981 attccagcta gtgatcaact gttggttcat ttcatccaca tttgcaaaga gccgcggagc
     2041 aaagtcgttt tttcctaata agcgttcatg gaaaaagtgc atatcactga taaagtaatt
     2101 catgtatctt ttgcctccct tttttcttag agtaaacaaa atcacttatc tgagcaactc
     2161 ataacatcta aaaagaaaag gttgtaaatg agacgtgcag ctttaagcga taagaagcaa
     2221 ttttgaaaaa tagtttttca caatttataa aattattcct tattgccgaa aggctggccc
     2281 tttttatgag tatgccaatt ttttatatcg aatgctcaaa agatgagtga ttttttggtt
     2341 gactatcatg tttgtgtact gaataggtcc agtagaacag tatttttacg acttgataat
     2401 taagggatga aattttcaga aatatgacgt ataatgtaaa atactgaaaa gttgcgacct
     2461 tcaactcgta gatgtttcag aatctaattg aaatgatggt gatatcatga cagacaacag
     2521 caaaacacgc gttgtcgtag ggatgagcgg cggtgtcgac tcatctgtaa cagcactttt
     2581 gttaaaggaa caaggatacg acgtgatcgg aatattcatg aaaaattggg atgacacgga
     2641 cgaaaacggt gtatgcacgg ccacggaaga ttataaggat gtagcgaaag tagctgctca
     2701 aatcgggatt ccttattact ctgtgaattt tgaaaaagaa tattgggatc gtgtatttga
     2761 atatttccta gccgaatacc gcgcaggacg tacacctaac ccagacgtga tgtgcaacaa
     2821 agaaatcaaa ttcaaagcat tcttggatta tgcaatggat cttggcgccg attatgtagc
     2881 tactggacat tatgcgcaag tcactcgcga tgaaaacggc gtggtccaca tgcttcgtgg
     2941 agtagacaat aataaagatc aaacttactt ccttagccaa ttatcacaag aacagctttc
     3001 aaaaacgatg ttccctctag gcggcatgga aaaatcagaa gtacgggcaa ttgctgaacg
     3061 cgcaggcctt gcgactgcta agaaaaaaga ttcaacggga atctgcttta ttggtgaaaa
     3121 gaacttcaaa caatttttga gcaactatct gccagcaaaa aaaggaaata tggtcactct
     3181 tgatggtgaa gtcaaaggac aacatgctgg cttgatgtat tatacgattg gtcaacgtca
     3241 aggactaggg atcggtggcg gaggagattc gcaagaaccc tggtttgttg taggaaaaga
     3301 tctagcgaca aacacattat atgtggggca agggttccat catcctgcat tgtatgccac
     3361 aagcttggat gcaagtgaaa tccactttac gacgaatgaa ccaatgccaa aagaattcaa
     3421 gtgtacagcg aaattccgtt atcgtcagca agacgttcct gtgacagttc gcttattgga
     3481 tgataatcgt gcagaagtcg tattcgacga acctgtacgt gcaatcactc ctggacaagc
     3541 agttgtattt tatgatggaa tggaatgtct gggtggtggt ttgatcgacc acgcatatca
     3601 agaaacaaaa gttttacaat acgtttaagc gatattaaat gaatcaaaac agctatggct
     3661 aacaaaagaa acaccttttc ttttggatag catagctgtt tttttgcaat aaaattaatt
     3721 tttattcatt ttatacttga aatacttgtg taaaaagttc ataatatcca ataatatgaa
     3781 taaagttgct ttttgttaag caagagcagt tgcaaaatag aagggaatct tttgcaagtg
     3841 atttgttcaa ttttataagt gagggtgatg gaaatggaaa agaaatcgac gggtattagc
     3901 aaatggggac tggttgctct ggttgtcagt tcgtcaatcg gaagcggtgt gtttgggatc
     3961 acaagtgatc tggctagttc tgcagctcca gggccagcga tcctttcatg gataatcgtt
     4021 gggattggta ttttagcttt ggtattatcg ctgaatcatt taggagaaaa gcgtccagat
     4081 ctagatggtg gtattttcgg ttacgcggaa gcttcatttg gtaaattagg tggcttcatc
     4141 agtggttggg gttactggtt gtctgcgtgg ctgggaaatg ttgcttttgc aacgatgctg
     4201 atgagcgcca tgggagaatt tttcccgata ttcaaaggtg gacaaaatgt tccttctatt
     4261 ttatttgcaa gtatttttat ttggggtctg actttattag taaacaatgg agttgaagga
     4321 gcgagcgtca tcaatacaat cgtgacgatc tgtaaattag tgccattatt cctattttta
     4381 atatttgtat ttatcgcatt taaagtgaat atattcactg ctgatttttg gggtaacgta
     4441 tctgataatc tagtgaatgg aacagggcag caaggaacga tctggttgca gattaaaggt
     4501 tgtttgatgg ttatgatgtg ggtctttgta gggatcgaag gcgcaagtgt tttagccaac
     4561 cgcgcgaaga aacgttcaga agcccagcaa gcatcgatca ttggtctgct ttgtttattg
     4621 tttatctata tccttgcttc gatcttgcca tacggcgtct tatctcaaga agagttagca
     4681 actatttcac aacctgctat ggcaaatatt ttaaaaggaa tcgtgggaaa ttggggagca
     4741 gcgctgatca atatcggctt gattatttcg atcattggtt cttggttgtc ttggacgatg
     4801 ctcccagcag aaacaacgat gttgatggct cgagacggtc tgttgccgaa aggttgggga
     4861 aaaacgaata aaaaaaatgc cccaacttat tcgttagttg ttactgcaat cttgactaat
     4921 ctattcttat tgactttctt agttactgat tatgcctatc aatttgccta ttctttatgt
     4981 acagcggcaa ttttgatttg ttatctactt gtcgggatct atcaagtcca gttttcttta
     5041 aaagaaggaa tcaaaaaaca agtagtgatc ggtgggattg ctgtagtttt tgaattgatg
     5101 ggcatcattt tagcaggatt ttcttatgtc cttttatgta gtattgccta tcttcctggt
     5161 ttttgcttct actggatcgc ttgtcgtgaa accaaacatc aaattacggg aaaagaaaaa
     5221 gtaatgattg ggctgatttg tgtggccgca ctgctttcaa tcgggttgct tgctgtaggc
     5281 tggatcaaga tataaggggg aaagaaacat gaaaatcgca aatttcggtg tagaagaatg
     5341 gctaaataaa tgggaaaaga aagcggtata tgatattgca caaagctcga tcgaagcatt
     5401 gacattagac gaactcgttg gcttagatgg gactagcgta agtaaatttt tcgaaaaacg
     5461 caaaaacgaa ccattggact atggctggat cgaaggatca gatgttttca aacaagaagt
     5521 cgcttctctt tatcaaaccg tcgatccgga aaacatctta caaacgaatg gagcgactgg
     5581 tgctaatctt ttagctctat acgcgttagt ggaagcagga gaccatgttg tttcgatgct
     5641 tcctacttat cagcaattat atgacatccc caaatcttta ggtgccgctg ttgactttgt
     5701 tcatttgaag gaagaagaag attggcagtt tgatttagaa cagctagaag ccttagtcaa
     5761 acctgaaaca aaaatcatct gtttgaatag tgcaaacaat cctacaggca cattgctaga
     5821 tcggaaaact ttagaaaaaa tcgcagaaat tgctcgtaca gtagatgcat atgtattgat
     5881 agatgaagta tatgcgcctt taacggataa aggtgaattc ttgtcgatcg tagatgtcta
     5941 tgaaaaaggt atagcaacca attccctatc caaaacctac tccattccag gtattcgtat
     6001 tggctggaca gctactggac ctgagttggc agaagttttt cgaaagtatc gagactacac
     6061 gatgatttgc ggcggagtcc tgtcagatga tcttgctgta catgcattga agaataaaga
     6121 aaaaatattg gcgcgaaaca aaaagatcat tacagagaac cttgtgattt taaaacaatg
     6181 ggtagccaat gaaccgaaag ttgaacttgt cgctcccaat tacgtctcta cttctttcat
     6241 taaattagcc atcgaagaag atgaccagac attttgtatc aatttattag aagaaacagg
     6301 agtactatta gtaccaggaa gtgcctttga tttaccgaag catgcccgac taggttactg
     6361 ttgtaaaaaa gaaaccttgg aaaagggatt attcctctta tctgagttct tgaaaaaaca
     6421 ggcctaaaca aaaacactgc aaggacagtc tgtgacaact gttcaagcag tgtttttcat
     6481 ttacgcttat tgtctatgag aaggagagtg ccctgaagct atggagactc ctgaagcttt
     6541 ttcttctgca gtttcttgct gatggatctc ttgtgtattg tgtcgtacaa gcaagaagtt
     6601 gaccaaaaca agaagagaag tcgaaagaaa gacgccgcga taaccaaatg cactggaaac
     6661 actggagccg atcataggcc ctactacgtt accagttgct tggaaagatt ggttataact
     6721 gaagatccgt ccagcagctt gatgaggcga atattttgtg atcaatgctt ggacagcagg
     6781 gagcagacaa gcatcagaga taccaatcaa aaaacgtaag gcagctaatt gccaaacatt
     6841 cgtgacgaat gccattggga tataaaccag aatcgcaaaa cccaaaccaa tcgcaagtat
     6901 tctttcactc ccaatcttgt cacctaagcg accaaatctt ggagcggcta tcaaagtggc
     6961 aatcccaggt atcgaagcaa tgattccgct gatcaacgta acattcccat gtccatgcag
     7021 caattggcga atgtacagac tgatgattgg actaatggaa ttgttggatg cttggatgat
     7081 catcgttgtg acgaacatac cgatgacgac gtgtggatat ttcaagtctt tgaaaatctg
     7141 tttggcagaa accatgtttt cttttttcac tgggacaaat ttttcatgga cgaaaaacag
     7201 actcaacaag aagacgaaaa ataagatgat ccctgtaatg aaaaaggtag ggcgatagcc
     7261 aaacgctgaa gcagagatcc cgccaagaag cggtccaagt aatgttcctg taacagaacc
     7321 agttgctaag gttccaagaa cttgaccact tttttctttt ggcgtcccag ttgcgacaag
     7381 ggcagtggca ttactgatat aaccagaaaa aatcccttga agcagacgca atgcgactaa
     7441 ttgatacaca ctagtcacgc aacccatcaa agaaatgacg attgccattc ctaatgaagc
     7501 acgcaacagc atcaattttc ttcctttttg gtcggctaat tttccccacc aaggagagat
     7561 aagcgtagtc accaagaaag tagatgaaaa agtcaatccg ctccaaaagt tcaattgtga
     7621 cgtactataa ttacctaacg tatctatgta aagtgacata aaaggcatga ctaaactaaa
     7681 tccgatacca gccataaatg ttccaaacca taagacaaac aagttttgtt cccaagtttc
     7741 tcttttccta aataattttt cctttattct tgcagccaat tagactcctt ctttctgaaa
     7801 attttcatct acagctcttt attataaact acaatatgta gtttatcaat gaaagtctct
     7861 tttttctctt tctttttcgt ggtaaactga aagagtgaag gatttatttt atgagaggaa
     7921 acatggaaat gaaaaaaaga aatctgtctc aagagaaaat catcgactgc tttcgagaac
     7981 tagcagaaga gatggatgtt cagcaaataa cctttcagca tttagccaaa cgcttggata
     8041 tcaaggctcc ctcgctgtat aaccatttca aaaatattcg tgatgtcaaa acagcgctaa
     8101 cagcgaaact tctgcaggaa cttaatgacc agctgcgacg ggctcttgtg ggaaaaagtg
     8161 gtcaggaagc actgcagatc tatgcagaaa cttatcgatc ctttgctttt tcaaatcaag
     8221 cagtgtatga gttactgatc agtgtaccgc ataccaatga agctgtttta ttagatggaa
     8281 tccatgaaac gaatcagatc attttgcaga tatttgaggc ttttcaatta acgaaagaag
     8341 aaaaagttca tcgaagcagg gaacttcgta gtatgatcca cggttatctt tccttacgtt
     8401 ttttaggata ctttacaaaa gaaccaacaa tcgatccaga agacagctat ttttggatga
     8461 tcaataactt tatcgctact ttgccttaaa caatcagatt catttgtaac aattttaaga
     8521 tgttcattaa atgacccttt tataagagaa aattcttgca actttcttga aaagtgacta
     8581 agatgagtct agaaaagctg aaagaaggag cacgagtatg tatcaagtaa ttaccatgtt
     8641 cggggacaat gagccttggt ggttttttga agactgggaa gaagatattg aagaagaaga
     8701 aacattcgag tctttcgaag aagcacgaca agcgtacgaa aaacaatgga acgaaatcca
     8761 tcaaaattat gaatatatca atgcaaaaag taactttctc agcgcctttt ggaatgacgg
     8821 agatgagcgc tggtgtgaag agtgtgacga tgatttacag caatacaagg gacttgcgtt
     8881 gttgaaaaat catcagccca tcacagtaga aagcagaaag gaattttatg aaacaactaa
     8941 ttctagcgga aaaaccaagc gttgcgaaag acctaagcaa ggtgcttggt gctaatcaaa
     9001 agcacaaaaa ttattatgaa ggacccaagg tgatcgttac ttgggccctt ggtcatttat
     9061 taggattgaa aatgccggaa gatctgaata aggaatggca gagctggcaa atggacacgt
     9121 tgccaatgat ccctaaaaaa ctaggaatca aaccacttcc taaaacagga catcaattaa
     9181 aagcaatcaa gcagctggct cagcgaaaag atgtctcaga agcagtcatt gcgacagatg
     9241 ccggaagaga aggggaactt gttgctcgct ggatcttaga gtatgtgcat ttcaataaac
     9301 cagtgaagcg gttatggatc tcttctcaaa cagataaagc aatcaaaact ggattcaagc
     9361 aactaagacc tgcgaaagaa tatgatgcat tgtatgaatc tgctcttgcc cgggcaaaag
     9421 cggactggct ggtaggattg aatgtgacca gagccttaac tgtgaagtac gcggataatt
     9481 tatcagcagg ccgtgtgcag acaccgacac tagcattagt gagagaccaa gaaaagaaaa
     9541 tcgagcagtt ccgtccacaa acgtatttca cgatcacgtt gcaagctgga aaagaacaag
     9601 ccaaaatgct tcaaaaaaat caatttgctt taaaaacaca agaagaagca caaaaacttg
     9661 ttcaattgat cagtacaagc aaaggaactg tcacagaagt ccaagaaaaa ttaaaaacgg
     9721 aatctgcgcc attgccttat gatttaacag agatccaaag agaagccaat caacgctatg
     9781 gctattctgc aaagaaaaca ttaagtcttg tacaaagttt gtatgagacc cataagatcg
     9841 ttacttatcc gcgtacagac agcaaatatc tgacaaatga catgaaaagt acgatgaaag
     9901 aacgattgca agcagtcagc gactttgctc ctgaggtcaa gcagtatttg aaaaacggag
     9961 ccatcgtcca acagacaaaa gtattccaag acagtaaagt gaccgatcac catgcacttt
    10021 tgccaactga aaacagagca cgttatgaaa aattaagtaa tgaagagcga aaaatctatc
    10081 aaatgattgt ctctcgtttt ttaggactgt ttgctcaacc ccataaagtc agccaaacaa
    10141 aagttacggt tgaatttggt aaagaacgat tcatttttcg gcaaaatcgt gtcgtccaag
    10201 caggatggaa aggcgaaact gaaactgaga cggaaacagt caaatgggaa aaaggaatga
    10261 gaatcaatcc cgatttcgcc atcaaaaaag aactatctgc accgcctaaa ccattgtcag
    10321 aagcaagctt gcttggtcaa atggaaaaat acagtctggg aaccccagct acgcgtgcag
    10381 agattatcga gaaactaatc aaatctgaac tgatggaacg tacccctcaa ggactgcaag
    10441 tatctcctaa aggtaagcaa ttattagtat tggtaaatcc ctctttagta acaccagatt
    10501 tgacagaaag ctgggaaaaa gaccttgaag ctattgctgc aggaaagaaa caagcaggaa
    10561 ccttcttgaa tggaatcgaa aaagaaacaa aacgcttggt gaatgagatc aaatcaagca
    10621 aacaagaata tcaagatttc tcgattaccc aaaagaaatg cccagaatgt ggggccaatc
    10681 tgcgcgagaa aaacacacgt gacggcaaaa tctatgtctg caccaattca gattgcaact
    10741 accgcagaag aaaagatcca aaagtctcta atcatcgatg cccgcaatgt caccggaaaa
    10801 tggaaatcat cgaaggtaaa aacggtgctt atttccgttg taaatacgat ggaacgacag
    10861 aaaaattgct tgataaaaaa gagcagaaga agaaaatgac caaacatgaa gaacgacgct
    10921 tgctgaaaaa atatgcacaa gcagaagaac cagaagaaag cccattagca gcagcactaa
    10981 aagcagcaat gaagaagtaa taagatatga aagaggttgt gatagaagtg gtcagcttca
    11041 agaaataaga aggaatttac gaaaattgct tctcaaattt ttgtgaaatt tcggcttatt
    11101 tccgaagaag ctacttctgg aacaccattt attcggaaaa aaggactgtg acaagacttt
    11161 tgtcacagtc ctttttcatt taaaaaaatg tttacttcat ttgacgtata ttattggtga
    11221 ggcttcaatt catttggctt cactttttct gtttctctta tgatataaac aggacgctgt
    11281 ttcgtttcta agaagatttt cccaatatat ttcccgatga ttccaagaca taacaattgg
    11341 attccaccaa tgaacaggaa gatcgagacc atggaaggcc atcctgatgt aggatcacca
    11401 aacaacaatg cccgcaaaat aatgaacaaa attgcaatac cagaaccgac acaagacaaa
    11461 gcgccgataa atgaagcaat cgttaacggt gcatccgaga agttcacgat accatcaatc
    11521 gagtagttga ataatttcca gaaggaccaa gatgtttcgc cagccacacg ttcgcggttt
    11581 tcataagaaa tatattctgt tttaaatccg acccagctaa ataatccttt tgaaaatcgg
    11641 ttgtattctg ttaattctag gacggcatcc accatttgac gagtcatcaa gcggaaatca
    11701 cgagcgccat cgaccatttc tgtatcgctg attttattga tcaactggta gaattttttc
    11761 gcaaagaaac tgcgtatcgc aggctcgcct tcacgagtcg aacgccgggt gccgacacaa
    11821 tcgatatcag aagtagtgat ccggttgatc atctcaggaa gcagttcggg cggatcttgc
    11881 agatcaacat ccatcacggt cactaaatct cctgtagaag cttgcaatcc agcgtacaga
    11941 gccgcttctt tcccaaaatt tctggaaaat gagacaaagc ggacataagg ataagctgag
    12001 gctagcttct tgattgctgc taacgtaccg tcttttgatc cgtcgtcaat aaacaaatat
    12061 tcgaactcat gggcaccttt gaatttttca acctcatcaa aaaaaagagg aacagttttt
    12121 tcttcattaa aacatgggac aacaattgat actttcatcg tttctatcct ttcaatcgta
    12181 tgtttatata tggtttacat tcaaaattta ataaacctta aataacacct ttaaaatttg
    12241 attttacgta aaagaacagg tattcttttt gtaaactaac gtatttagtt taccatgttt
    12301 cctcggttta tcaaactttt aagaacaatt tttattagtt ggcaagtagt tttggataga
    12361 gaaaaaggag gaagttccag tctattgact aggagcaaac aaaatgaaaa tacacacacc
    12421 tatcggcttt ttatggttag atgcagatga atccggattg actgctgtat cttttcagcc
    12481 aatttcagaa agcgaatcag cgaatcagcg aattttaacg cgcacacaag aacagtttga
    12541 agaatatttt gcgggaaaaa gaaaagaatt tgatgttcct ttatctataa agagaggtac
    12601 gccttttcag caaaatgtct ggaaagcttt gtcgaccatt ccttatggag aaatccgaac
    12661 gtataaagaa gtagccgttg ctgttgatag tccgaaagct gtgcgagcga ttgggcaggc
    12721 aaatcgaacc aatccattgc caatcgttat tccttgtcat cgagtgatcg ggcaaaatgg
    12781 aaaactgaca ggatatatgg gaaacgcaat agatggggtt gaaatcaaac gccaattgtt
    12841 gttgttagaa ggatatctgc aaaattaaaa acagttgtga ttcggacatt gttccatgcg
    12901 agaatcacaa ctgttttttc atgttcattc taaactttga tcagccaaat aaatagtaaa
    12961 gccatcactg tttggagaag cccgacagct aatccataag ataagaagcg tgtaccttct
    13021 ttcatgaatt ttgcaaagtc taaacgcaga ccaattgccg ctaaagcagt gatctcaaac
    13081 cagctgctga taaaatgtgc cgattggtca ataactacag gtaaagaaaa caagctgttg
    13141 accaaacaaa agatcacaaa ccctaataca taccaaggaa gcggtaattt tcgttttttc
    13201 tgcgtatcag ttggcgtttc aaacagagct ggttgtttct tgtgcatgaa tttttcaaac
    13261 agatagacaa caacaacgag catcataata cgtagaatct taaatagcat ggcgaattcg
    13321 acaacagaat tgtcaagtaa gcttgcccca gcgactactt gaccaacaga ttggagtgta
    13381 ccacccaaaa gtgcactttt ttcaatgaga tttccaccaa acaactgtaa tccaagaaaa
    13441 gggagagtca acatcatcac agtacccagt agattgacta gtgtgatgat ctgtcctttt
    13501 tcttcctctt tcgcatggat ggaaggggcg attgaagcaa tagcagaaga accacaaaca
    13561 gcgtttcctc cagccatcat caaagaagaa gtatcgctga attgcagttt tttaccaatc
    13621 aagtaagcac cgatgatagt gactgacatt tgcagtaaca caaaaacaat tccttttaca
    13681 ccaatttgag caatcgtttg agctgtgaca gttgcaccta gtaaaaaaac agagaattct
    13741 aatagtttgc tttcagcaaa ttttgtgccg attgctagaa caggctgttt aaaaaaagta
    13801 tttcctaata aaatacccaa taaaatagca atagtcgctc cacctaactg tggcagataa
    13861 atagcaagaa gcctgctgaa ggcagcgatg gcaaaagaaa gcagtaaacc gggtaagatt
    13921 tcgcggaccg ctgaatcctt tacagatttt attggatgtg acaatgattt catttgatcg
    13981 gttcctttcg tcaataaata ctaaaagcat actcgaacat agtagcacta aaccagtatt
    14041 ttgacaaaag aacaagaggt cgtgacaaaa gtcttgtacg acctcttgtt tcgaataaac
    14101 ggtgttccag aagtagcttc tacggaaata agccgaaatt tcacaaaaat ttgagaagca
    14161 attttcgtaa attccttctt atttcttgaa gctgaccact tcttttacga cctcttggta
    14221 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccagtacaaa aacatgaaca
    14281 gctgtttttg tttggccgtt cttattactt gatgcagaac agctttttca caacctattg
    14341 aatcgattat taagcgattt ttttagaaac aaattttcca tgtttgctat gttctttaat
    14401 taagaaacgt acgtaatgag caagaacgta gaagacaact acacttgcaa acagacattc
    14461 cgcaactttt aagaaatcca atacgctact atagatacct aaactgttga agtaatgaac
    14521 agtagtaaat atagctgttg cagagataac aagcgggaat gtaaaggctg catagcttgg
    14581 ataaaaacga agcttgaaca aacgtggaag catagacagc acaacgaaat acaatacttg
    14641 agataaagcg aacaatgcta cgacaagata aaaattaggt tcagcaaagt ttttaagata
    14701 acctgttagg caaagagaac caggtgctgc aacgatcgta atcagcggac gtgtcggatg
    14761 agccatattt cttacgaaaa agacacgttt gatgatgatc ggcaataaga acgcatagca
    14821 gatcaatgaa atccaaaaga aaatttttcc aataagtggg aagaagttcc cggcagttac
    14881 agtgatgacc ccaaatccaa cgtaaatgat aaaccagctt ggataaattg cttcaattga
    14941 aagatcagct ttgataacat ggtgataatt gaaataaaag acaaggatca cttggaagat
    15001 aacagcgcac agccaaatga atttgacaat tggtgcaata ctttcatatg aaacaaaata
    15061 agtacaaata accatgagtg acatcgtaaa agttggtgaa acggaggcga tgattggatc
    15121 atgcaacgtt tgtttcgtgt gttcgaataa gataaacaat ttaacaaaaa tgaatatcat
    15181 aagaatcata gagataccgc cagttagatt gcctaaagca acatggtgat agtctttaaa
    15241 cagatttcca agggaagcta acccaaggat caacccgcag atcgggatcg gaataatttt
    15301 caaaaagttc ttcatatata atattctcct ttttatttaa tagattttag taccaagatg
    15361 aacgaataca ttgtaaaatg aataggaatg tatgattacg agcaagcaga cagctgattg
    15421 tcttcatcta agttactagt tggatcagca actgttttgt tgtacatttc tattatagat
    15481 ggttttttgt atatttaaat caaatagttt ttatagtaat tatcataaaa agttatggaa
    15541 gtggtgccat gtttaagtta ttaacgactt ttcgagcagt atatgaaaca agaaattttt
    15601 ccagagcagc agaagtactg tttttatctc aaccagcagt ctcgaatcaa atcaaacagt
    15661 tggagcggga attgaatatc gatctttttc aacgtaacgg aagaaaagag atgcttccaa
    15721 ctaagcaagc cgacttatta tatgaacgga cactggtttt gttagaagaa tgggaagaaa
    15781 ctcagcaaaa attgctcaat gaacaaaatg aagaagaagt atgtcggatc gttgcttccc
    15841 acacctttgc tgtctatctt ttgcctcagt tgatgaaaca tctgatcaaa aggtttccta
    15901 aagtgcattt tgagatcgaa atcacgaatt ctcatgaatc attagaacaa gtagcgaaac
    15961 atgaatccga ttttggtttt atcgaaaagc cattagagac ttccggcgta caaagatact
    16021 ctttgatgga agaccagcta gtcatcgctg gggacccaaa aagcaaattg tggcttgtaa
    16081 gagaagcaaa ctctggtgtt tttcattaca cgcagcgtta ttttgaagag atgaatatcc
    16141 aagataagaa gatgatcgtg aaaagtaacg atgtaattgt tgcgttgctg aaggaaggaa
    16201 tcggtcgctc gattttgtct aagcgctctg ttccagaaaa tgtgccaatg actccgttga
    16261 atttccatcg atatttttat ttcatccaac gaaccaatct ggtttctgat cagctgctag
    16321 agattgccga agagatcctt cgatacaatg aaagcacaga aacttctcac agcgaggaga
    16381 atctcagctg gtagataatt gcatgtatca aaaaaagttc tatgatacac taaagataag
    16441 cagtaggctt ttgtactgca tttgctcaag caactgcaca aacggaaagt taatttttcc
    16501 caaatgtcta tggaaagact ttccattatc taactggacc gctataataa tcagcagtat
    16561 ggtttttaag aaaagggatg aaacatggaa aaaaaattaa aagcttcgaa cgataatcgt
    16621 attgattatg gcgtgatttt accagtcttt ctgttatgtt tgattggtct gctctcatta
    16681 tatgtagcac ttagccatga tccgaacaat ccagcagtct tgaaaggcgt agggatgcaa
    16741 gttgtctggt atttagttgg agcggcagca atcgttgtca tcatgcattt gaattccaaa
    16801 tggatctgga aactgactcc ttacctttat ggtttggggt tagtggtaat gggactcttg
    16861 ctcaaattct atgatcagaa tttagctgct tcaacagggt ctaaaaactg gtttcgattc
    16921 ggtgcgttta ctttccagcc agcagagttg atgaagattg cgtacatctt gatgatggca
    16981 ctgatcgtaa ctaaacataa tacgcaagta aaagaacgga cgatgaaatc ggacttttgg
    17041 ctgattggga agctcttgat cgtgacaata ccggttttag cattgatcat ggcccaagat
    17101 gactttggaa cgatgcttgt ctttcttgcg atctttggcg ggattttcct gatgtctgga
    17161 atctcttggc gcatcatcgc acctgtagta atcttggcag tagtcgttgg agcaggaacg
    17221 atcttcttgg ttacgactga aggcggaaga gacttgcttt ataaagttgg attcaaatct
    17281 tatcagtttg cacgtattga ttcatggctg gaccctttcc atgatacgtc aggaatgagt
    17341 tatcagccgg cccaaggact tttggcaatt ggtaccggtg gcttgtttgg taaaggattc
    17401 aacgtaagca atatctatgt gcctgttcgt gagtccgata tgatttttac cgttatcggt
    17461 gaaaacttcg gctttatcgg tggtgcattc gtcattttct tgtattttat tttaatctat
    17521 cgtatgattc gggtgtgttt cgatacgaat aatgaatttt acgcctatat cgcaagcggt
    17581 ttgatcatga tgctgctgtt ccatgtcttt gagaatattg gagcaaatat cggattactt
    17641 cctttaacag ggatcccatt gcctttcatt agtcagggag gatcctcgat tttagggaac
    17701 atgatcggta tcgggttgat attatcgatg agataccaaa atgaagcacc tgcaagatct
    17761 tcaaggaggt aagaaaattg tacacctttt actggtaccc gaaatgttca acatgcaaaa
    17821 aagccaaagc gtggttagac cagcatggcg ttagttatca aaccgttgat atgatcaaag
    17881 aagtaccgaa ggcctcaacg attgagactt ggctagaaac gagtcaagta ccaatccgtc
    17941 gcctgttcaa cacaagcggg atgaaatatc gggaacttgg actgaaagat caagtaggcg
    18001 agtttacggc ttcaaaagcg agccaagtct tatcgacaga tggtatgctg atcaagcgac
    18061 cactgatctt acgagaggac cagcttgttg caattggatt caatgaaaaa atttatgaag
    18121 gagaactgat ataaaatggc aggcaagcat ttgaagaaaa aggacaacct ttggatctta
    18181 tttaatggaa aagaatactg tgttggtttg acaaatgaag cgcaagaaga acttggagac
    18241 atcacgtttg ccaatctgcc aaaagcagga caatcgctaa aaaaagatga acctctgatc
    18301 gaagttgaag cggaaaaagc ggtcaatgaa tttgccagcc cgctaacggg tacagtttct
    18361 tctgttaatg ataaaatcgg tgaagatatc aatatcttga atgattcaga tgaaatgaat
    18421 gcgtggatct taagtttcaa agatgttgat ccaaaagaat ttgacgaatt ataaaatcag
    18481 aaaatcactt gaaaaaagtt gacactgatt cttccagttg ctataatcat aagcaatcct
    18541 atatgaaacg agaaagcttt gaaaagaaga gtacatgtca cggacgtttg aagagagccc
    18601 tgtttgctga aaatgggtaa cgaaaatgag atggaagatg gtcttagagc agagtaagcg
    18661 agcgtaagca aacttactac gggagtgccc gttacagcgc gacagtatac ggaaagaagc
    18721 taagtccaag tactggaaga ggctgttatt gcgaagtaac ggtaaatcaa ggtggtaaca
    18781 cggaagttca agctttcgtc cttttgctta aggcaagagg gcgaaggctt ttttgtttga
    18841 aaaattataa gagaaagatg tgaatggcat gtcattaatc caactaacga atgtcaaaaa
    18901 gacattcaat gggaaaaacg gaaacatcca agccgtcaat gacgtttcac taaacgttga
    18961 aaaaggggat atttatggaa tcgtcggcta ttctggcgca ggaaaaagta cattagtccg
    19021 tctcctgaat ggacttgagc ttcccacgag cggcgaagtg atcgtaaacg atcaagatat
    19081 tacaaaatta aaaaacaaag aattacgtac attccgaaag aaaatcggta tgatcttcca
    19141 gcatttcaac ctgttatggt caagaactgt acttgaaaat atccagctgc cgctggaatt
    19201 agctggcgta ccaaagagca aacgtaaaga acgggcagaa gaactgttgc gtttagtcgg
    19261 gttagaagga cgcggacaag catatccttc acaactttcc ggcggacaga aacaacgcgt
    19321 cggtatcgca cgagcattgg caaatgatcc tgagatcctg ctttgtgatg aggctactag
    19381 cgcattggac ccgcaaacaa cagaagaagt attagactta ttgttagcga tcaataagaa
    19441 attgaatttg accatcgtct tgatcacaca tgaaatgaat gtgatccgca aaatctgcaa
    19501 caaagtcgct gtgatggagt tggggaaagt cgtagaagaa ggagacgtat tgactgtctt
    19561 cagacaaccg aaaaaagaag taaccaaacg ctttgtacaa caagacatcg aaccagaagc
    19621 tgattcgaca gaactattag ctgaactcat caaagaaaat ccaagcggcc gtttggcaac
    19681 tttgaccttt agcgaaagaa atgccaacga gccagtcatt tcccaagcga ttcgacgcta
    19741 taacatcgat gtcaacgttg tgtatggaaa gatcaaacaa gtgaaagaag gttcctttgg
    19801 ttcattgact gtcttgatga ctggtgaaac caaagaatta gatcaagcag aagtgttcat
    19861 caaagaacaa ggagtaggaa ttgaggtgat ccatcgtgga taaaagcttt tcagaaacat
    19921 atctagattt taaacaaatc aacccggaaa cgatgaatca ggctatcctt gatacactat
    19981 atatgacatt aatctcaatg gtcttagtga cgatcatcgg attagttttg ggattgatcc
    20041 tttactctat tggacgcaaa aacaaaccgt atgctcgaat catttatgga atcgtatcga
    20101 tcatcagtaa tattttccgg tcaacgccat ttatgatcct gatggttttg atcattccat
    20161 ttactaaagc aatcgtaggc actatgctag gtgcgcaagc agccattcca gctcttgttt
    20221 tatccgcagc accattttat gcccgtctag tagagattgc ttttcgtgaa gtcagccctg
    20281 gtgtactgga agcagcagat gcaatgggag caagttactg ggaaatcatc tggaaggtct
    20341 tgatacctga aagtattccc gcattgattt ctggattaac agttacaacg atctcgatga
    20401 tcggatttac tgcgatggcc ggcgcgatcg gcgcaggtgg acttggaggt cttgcttggc
    20461 aggaaggcta ccagcgtggt aatctaacag tgacattcgt tgcgacttta attattttac
    20521 taatcgtatt tatcgttcaa ggaatcggtg atttcttaac taagaaaaca gacaaaagat
    20581 aataggatat agggggatgt aggatatgaa aaagaaaatt tttggatttg cagcagcgct
    20641 tttgctgact gtaggattag cagcatgcgg taacaacagc gacagcaaag attcagcaaa
    20701 taaagcagac gacacgacat tgaaagtcgg cgcttcaccg acaccgcacg cagagatttt
    20761 ggaacatgtg aaaccattat tgaaagatga aggaatcgat cttgaaatcg tcaaattcga
    20821 tgactatgta ttaccgaacc aatctttgga agaaggcgac attgatgcca attacttcca
    20881 gcatattcct tatttgaaca aagaaatcaa agaaaaagga tatgactttg tcaatgccgg
    20941 tgctgtccac atcgaaccaa tgggcttgta ctctaaaaaa gtcaaagatg tttccgaatt
    21001 aaaagatggt gcaacaatca ttacttcaaa ttctgaatca gactggggac gcatcatcac
    21061 aattctacaa gatgctggct tagtcaaagt caaagatgga gtagatctgg aaacagcaac
    21121 attcgatgat attgaagaaa accctaagaa cttgaagttc aaccatacga tcgacccatc
    21181 acttttagct tctacgtacc aaaatgatga aggtgaccta gttgcaatca acgcaaactt
    21241 cgcttacggc gcagggttga atccattaga agatgccgta ttgcttgaaa aggacaactc
    21301 tccttacgtg aatattattg cgactcgtaa aggtgatgaa gacagtgaca aaatcaaaaa
    21361 actgattgaa gtgcttcacc gcaaagaagt ccaagaatgg atcacagaca aatggggcgg
    21421 ctctgttaaa ccagtaaagg ctgatgcaaa ataaggtatg gaacaaaaaa gatgagaaat
    21481 aagtctgtaa tggcttattt ctcatctttt ttatacctat tctaatttag aaatcaaaat
    21541 tgtttaataa taaataaaag atatttgatt ttagtaagga aggtcattgc gcttcattct
    21601 caattagtct aaaatgagtg agaacgaaag agaatttggg ggtataatgc atgtctgtct
    21661 tagaaataaa aaatctacat gtgtcgatcg aggaaaaaga aatccttaaa ggcgtaaacc
    21721 taacaatgaa aaccggagaa atccatgcca tcatgggacc taacggcaca ggaaaatcta
    21781 ccttatctgc cgctatcatg ggcaatccaa actatgaagt aacagaaggc gaaattttgt
    21841 ttgacgggaa aaatgtttta gacttggaag tagatgaacg tgcgcgactt ggcttatttt
    21901 tagctatgca atatccaagt gagataccag gtatcacgaa tgcggaattc atgcgtgcag
    21961 cgatcaatgc gaaacgtgat gaaaacgaca aaatttctgt tatggcattt ttgaaaaagt
    22021 tagatgaaaa aatggcttta ctaaatatgc cagaagaaat ggctgaacgt tatctgaacg
    22081 aaggtttttc tggcggagaa aagaaacgga atgagatcct gcaattgttg atgttagaac
    22141 ctacatttgc aattttagac gaaatcgatt caggtctgga tatcgatgct ttgaaagtcg
    22201 ttgctaaagg agtaaatgaa atgcgcggag acaatttcgg tgcattgatc atcacccact
    22261 atcaacgatt attgaactat atcacacctg atgtcgttca catcatgatg gaaggccgag
    22321 tagtcatgac agggaatgct gacctagcta aacgtctgga agcagaaggc tacgctggta
    22381 tcagtaaaga attaggaatc gactataaag aagaagaagc gtaaggggga gcaagaatga
    22441 aagaaaaaaa ctggctcgat tatcttgacg cggtaaatga tttttcttta agtaaaggcg
    22501 aacctgattg gatgagaacc ttccgtcaag acgcactagc gaaagcagat gaactgccat
    22561 tgccgcatat cgatcgggtg aaattccatc gctggtcgtt atttgatgtg aaagaaacac
    22621 aaaccatcag tgaaacaggc acgattccgg catttgatgc aatgaaagac aatcctgtgc
    22681 ttgtccaaca aggttcatgg acgatttttg aacaactgcc tgtggaatta gcagaaaaag
    22741 gagtcatttt taccgacttg ttcactgcaa tgatcgaata tccagaacta gtccaagaat
    22801 attatatgaa aaaagcagta aacatgaacg aagaccaatt gactgcactc catgtagctt
    22861 ttatgaacag cggtattttc ctgtatgtgc ctaaaaatgt cgtgatcgat gaaccactcg
    22921 agtccttatt cattcaagat ggcgcatccg atgagcactt cttcaagcat gtgttgatcg
    22981 tggcagatga acatagtgaa ttttcttatc tggaacgttt ccagacaacc aaagaacaag
    23041 tagcaaaatc atcaggtaat atcatcgtgg aagtcatcgc taaagctggt tcaaaaatca
    23101 aatattcagc cgtagatcaa ttaggagaaa atattacttc ttatatgaat cgccgtgggc
    23161 acattttacg agatgcttct gtcgactggg caattggtgt catgaatgac ggtcatgtga
    23221 tcgctgactt tgactcagat ttagcaggag aaggtgcaca cgcagaagtc aaaatcgtgg
    23281 caatcagctc gggcagacaa atccaaggaa tcgatacacg agtaaccaat aaagcccctc
    23341 atacgatcgg gcatattttg caacacggag tgatccgaga aaaaggaaca ttgaccttta
    23401 atggtatcgg ccacatcttg aaaggggcaa aaggtgcaga tgcccaacaa gaaagtcgtg
    23461 tcttgatgct ttctgataaa gcacgtggtg atgcgaatcc tattcttttg atcgatgaaa
    23521 acgaagtaac tgctggacat gctgccagtg taggacgagt tgatccagaa gaaatgtact
    23581 atctgatgag tagaggattg cataaagaag aagcagagcg cttagtgatt cgaggattcc
    23641 tcggttctgt cttaacagca atccctgtag aacaagttcg gaaagaatta gtcgaagtaa
    23701 tcgaagggaa gttgaatgga tgatcgatgc taaaaagcta cgcaatgatt ttcctatctt
    23761 atttcaagac gtcaatgatg aacctttagt ttatctggat aatgcagcga cgacccaaaa
    23821 gccaaaacaa gtactccagg cattagcgta ctattacgaa cacgataacg ccaatgttca
    23881 tcgcggtgtc catacgttag cagaacgcgc gacccatgat tatgaacaag caagagaaaa
    23941 agtacgggcg ttcatccatg caaaagaaac agcagaagtg ctgtttactc gtggtacgac
    24001 gacaagcttg aattgggtag ccagaacctt cggcgaccgc tatatcaaag caggagacga
    24061 gatagtgctc tcttacatgg aacatcattc taatgttatc ccgtggcagc agttggcaaa
    24121 acgaacaggt gctgtcttga aatatatcga actaacagaa gaaggcttcc tggatatgga
    24181 ggatgcagcg aaaaagatta cggacaaaac caaaatcgtc tccattgctc atgtatccaa
    24241 tgtcttaggt gtcttcaatc ctgttcaaga attagctgaa cttgtccatg aaaacggcgg
    24301 ggtcatcgta gtagatggtg ctcaatctgc cccgcacatg gccattgatg tccaagcact
    24361 agactgtgat ttctttgctt tcagcggaca caaaatgtgc ggaccaacag gtattggtgt
    24421 tttatatggt aaacgaaaat ggttagaaga aatggaacct gttgaatttg gtggcgaaat
    24481 gatcgatttt gtcgaattat acgacagtac atggaaagaa ctaccttgga aattcgaagc
    24541 agggacacca aatatcgccg gcgctatcgc attaggccat gcaatcgatt atttgcaaaa
    24601 actcggtatg gacaatatcc atcgatatga agaagaacta gccgcttatg tgttgccaaa
    24661 attacaagca attgatggat tgactgttta tggtccacaa gacccgaatt ttcggacagg
    24721 agtcgttgca ttcaatttgg atgggttaca tccccatgat gtcgcgacag ctttagacat
    24781 ggaaggggta gccgtcagag caggtcatca ttgtgcgcaa ccgctattga agtaccttga
    24841 agtatcagcg actgctcgcg cgagctttta tttctacaac acaacgcaag acgccgaccg
    24901 tttagttgaa gcaatcatgg caacaaagga gtttttccaa catgggacta tctaaattag
    24961 acaatttata ccgtcaagtg atcttggatc attcttccca tccgcatcat cgtggaagct
    25021 tgactgactc gacaagcaag atcgaattga acaaccccac atgtggagat gtgatcgaat
    25081 tgcaagtcga tgtaaaagac ggaatcatca aaaatatcgc ctttgacggc agtggctgct
    25141 ccatcagtac ggcaagtgcc tcgatgatga cagacgccgt catcggaaaa tcaatagaag
    25201 aagcagaaca tttaactgta gactttctac agcttgtcca aggaaaagaa gtcaaagatc
    25261 ccgaccaatt aggcgatgct gctatgcttg gtggtgtagc taaatttccc gctcggatca
    25321 aatgtgcgac acttgcctgg aaagcactag gaaaagcaat ggaaaacgat ggtaccgcag
    25381 ctttccatca ttaccacaag gaagaggagt gagaacatgg gcgtaccaga attagaagaa
    25441 tataaattcg ggtttcacga tgatgtcgaa ccggttttca gtacaggaga aggcttgaca
    25501 gaagaagtga tccgtgagat gtcaagaatc aaaggcgaac cagaatggat gcttgaattc
    25561 cgtctgaaat ctctagaaac atttaacaaa atgccaatgc aaacatgggg ccccgacctt
    25621 tcagacatcg atttcgatgc catcaaatat taccaaaaac caagtgacaa acccgctcgt
    25681 gactgggaag acgtaccaga aaaaatcaaa gatacatttg aaaagattgg tataccagaa
    25741 gctgaacgag cttatctagc cggtgcttcc gcacaatatg aatcagaagt cgtgtaccat
    25801 aatatgaaag atgaattcga aaagttagga atcgtcttta ctgacactga ctctgctttg
    25861 aaagaatacc ctgaattatt caaagagtac ttttcaaaac ttgtaccgcc aaccgacaac
    25921 aaattagcag cgctgaattc agccgtatgg tcaggtggga cctttattta cgtgccaaaa
    25981 ggcgtcaaag tcgatgttcc attacaaacg tacttccgga tcaatgcgga aaacacgggg
    26041 caattcgaac gtacgctgat cattgttgac gaaggagcaa gcatccacta tgtcgaaggt
    26101 tgtacggcac ctacttattc aagtaacagc ctgcatgccg ccatcgtaga aatctttaca
    26161 agaaaagatg cgtattgccg ttacacaacg atccaaaact ggtcagacaa tgtgtacaac
    26221 ttagtaacca aacgagcaaa agcatacgaa ggcgcaaccg ttgaatggat cgacggaaac
    26281 ctaggtgcaa aaacaacgat gaaataccca agtgtttact tagatggaaa aggtgcacga
    26341 ggcaccatgc tctccatcgc ctttgcagga gcaaaccaaa tccaagatac gggtgcaaaa
    26401 atgatccaca atgcaccaaa tacatctagc tctatcgtct ctaaatcaat ttcaaaagac
    26461 ggcggagaag taaactatcg aggacaagta acctttgcca aaaacagtgc gggttcgatc
    26521 tctcatatcg aatgtgacac gatcatcatg gacgaatggt cgaaatccga tacgatccca
    26581 ttcaatgaga tccacaacag ccaagtctct ttagaacacg aagccaaagt ctcaaaaatc
    26641 tcagaagaac aactctacta tctgatgagc cgaggcctga ccgaatctga agcaactgaa
    26701 atgatcgtca tgggctttgt tgaaccattc accaaagaac ttccaatgga atatgcagtc
    26761 gaactcaatc gattgatcag ttatgagatg gaaggcagtg ttgggtaaac ctgttatagg
    26821 agctagaacc cttgatatat
//
LOCUS       DAJSKS010000023        26164 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJSKS010000023 DAJSKS010000000
VERSION     DAJSKS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.023"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(66..1097)
                     /gene="queA"
                     /locus_tag="ON396_000852"
     CDS             complement(66..1097)
                     /gene="queA"
                     /locus_tag="ON396_000852"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR3112979.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1290..2465
                     /locus_tag="ON396_000853"
     CDS             1290..2465
                     /locus_tag="ON396_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3112980.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2469..3110
                     /locus_tag="ON396_000854"
     CDS             2469..3110
                     /locus_tag="ON396_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3112981.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3107..4024
                     /locus_tag="ON396_000855"
     CDS             3107..4024
                     /locus_tag="ON396_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3112982.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4021..4692
                     /locus_tag="ON396_000856"
     CDS             4021..4692
                     /locus_tag="ON396_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3112983.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5233..5721)
                     /locus_tag="ON396_000857"
     CDS             complement(5233..5721)
                     /locus_tag="ON396_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HCR3112984.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5922..6188)
                     /locus_tag="ON396_000858"
     CDS             complement(5922..6188)
                     /locus_tag="ON396_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR3112985.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6188..6451)
                     /locus_tag="ON396_000859"
     CDS             complement(6188..6451)
                     /locus_tag="ON396_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR3112986.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            6927..7112
                     /locus_tag="ON396_000860"
     CDS             6927..7112
                     /locus_tag="ON396_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3112987.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7254..9077)
                     /locus_tag="ON396_000861"
     CDS             complement(7254..9077)
                     /locus_tag="ON396_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCR3112988.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9675..12380)
                     /locus_tag="ON396_000862"
     CDS             complement(9675..12380)
                     /locus_tag="ON396_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCR3112989.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12686..13633)
                     /locus_tag="ON396_000863"
     CDS             complement(12686..13633)
                     /locus_tag="ON396_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCR3112990.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13823..14092
                     /gene="rpsN"
                     /locus_tag="ON396_000864"
     CDS             13823..14092
                     /gene="rpsN"
                     /locus_tag="ON396_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR3112991.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14268..14630)
                     /locus_tag="ON396_000865"
     CDS             complement(14268..14630)
                     /locus_tag="ON396_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR3112992.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14641..15762)
                     /locus_tag="ON396_000866"
     CDS             complement(14641..15762)
                     /locus_tag="ON396_000866"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR3112993.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15778..16128)
                     /locus_tag="ON396_000867"
     CDS             complement(15778..16128)
                     /locus_tag="ON396_000867"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR3112994.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16258..17769)
                     /locus_tag="ON396_000868"
     CDS             complement(16258..17769)
                     /locus_tag="ON396_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR3112995.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18091..19443)
                     /locus_tag="ON396_000869"
     CDS             complement(18091..19443)
                     /locus_tag="ON396_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR3112996.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19596..20672)
                     /locus_tag="ON396_000870"
     CDS             complement(19596..20672)
                     /locus_tag="ON396_000870"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR3112997.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20868..22613
                     /locus_tag="ON396_000871"
     CDS             20868..22613
                     /locus_tag="ON396_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HCR3112998.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22893..23330)
                     /locus_tag="ON396_000872"
     CDS             complement(22893..23330)
                     /locus_tag="ON396_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR3112999.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(23314..24687)
                     /locus_tag="ON396_000873"
     CDS             complement(23314..24687)
                     /locus_tag="ON396_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113000.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(25009..25497)
                     /locus_tag="ON396_000874"
     CDS             complement(25009..25497)
                     /locus_tag="ON396_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3113001.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25519..26121)
                     /locus_tag="ON396_000875"
     CDS             complement(25519..26121)
                     /locus_tag="ON396_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR3113002.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         7503 a         5561 c         4838 g         8262 t
ORIGIN      
        1 ttttctttgt cataaaatct ttgtaaagct tttttgttat tcaaaactag cggcttttca
       61 taaccttatt tcacgaacat cgcatctcca aaactaaaga aacgatagcg ctcttcaatc
      121 gcatgctggt aagcagataa agtcaattct cttcctgcaa aggcactaac taacatgact
      181 aaagtcgatt ttggcaaatg gaaattagtt gagaaagctt ctaccacttt gaattcatag
      241 ccaggcgtaa tgaaaatatc agtccatccg ctgtctgctt tgatttctcc attaaatttc
      301 gttccaatcg tttctaatgt tcgaattgaa gttgttccga cagccacgat tttcccgcca
      361 tttttacgta cttggttcaa tcgctcagcc gcttcttccg tcaaacggta gaattcacta
      421 tgcatatggt gttcttcaat tttatctacg ctgacaggtc ggaatgtacc taaccctaca
      481 tgtagtgtca gatagactaa ttcaacaccc tttgcttcga tctcatcaag taattctttc
      541 gtaaaatgca atccagctgt tggcgctgca gcagaaccat tttctttcgc atagacagtt
      601 tgatagcgat ctgggtcttc cagacgttct ttgatatatg gaggcaatgg catttctccc
      661 aatgattcaa gaatctctag aaaaattcct tcatactcaa agtcaatgat ccgtccgccg
      721 tgttccaatt cttctttcac tactgcttgc aatctgccat caccaaaaga gattcttgtc
      781 ccaactttgg cacgtttcgc aggtttgatc aatgtctccc acgtatctcc ttgtgtattc
      841 gttagaagaa ggacttctaa atgcgcacca gtatctggtt tttctccata taaacgagca
      901 ggcagaactc gtgtattatt cataactagc gcatcaccag gctgtaactc ttcaatgatt
      961 tcatggaaat gtttatcttc catctctcca gttttccgat ccaaaacaag taaacgagaa
     1021 tgatcgcggt cttttaatgg tgtttgtgcg attaattctt ccggcaagtc aaaatcaaaa
     1081 tcttctgttg ttaacatatc gtcactcact ttctatcatg tgcttcatca ttctatcatg
     1141 tttttcgttc tgttggcaaa atcagcactt tttcacttct attttttgtt ttcctaaata
     1201 aaaaaactac cctatttgta ttttttcatt gttttaggca tactagtaat agatagttct
     1261 tcattgaaaa tagaaaaaga ggtggcacca tgattgaatt tcaaaatgtg tcaaaaattt
     1321 ataaaggtgg aaaagtcgct gtagaggacg tcaatctttc atttgaaaag ggtgagttca
     1381 tttgttttat cggtacgagc ggaagcggaa aaacgacgac gatgcggatg atcaaccgaa
     1441 tgactgatcc aacacaaggg aaaattttga ttgatggaaa atccatccaa gaaattgatc
     1501 cagtggaatt gcgacgtcag atcggctatg ttatccaaag tattggattg atgcctcaca
     1561 tgacgatccg tgaaaatatc actcttgtcc aaaaattgtt gaaagtcagt caagaagaac
     1621 ggaataaaac agctgaaaaa atgattgatc ttgtggaatt gccccgtgag atgttagatc
     1681 gctacccaca tgaattatct ggtggtcagc agcagcgtat tggagtcgtt cgtgcccttg
     1741 cagcgaatca agatatcatc ttgatggacg agccattcgg tgcgcttgat ccaatcactc
     1801 gtgattcctt gcaagacctt gtcaaagatt tacaagagcg tttgggaaaa acgatcgtat
     1861 ttgtaaccca tgacatggat gaagcgatta aactagcaag ccgtatcgcc atcatgagtg
     1921 aaggccgtgt gatccaattt gatacaccag aaaatatttt acgtcatcct gcaaacgatt
     1981 tcgtagaaga gttgattgga gaagatcgcc tactacaagc caagccaaat gcgacaagag
     2041 tcggcgaagt tatgctaaat acagcaatca cgattacacc ggaaaaatca ttacaagaag
     2101 caatcaaact gatgagggaa aaacgtgtag atacgttgtt agttaccgat aattcgaatg
     2161 tattaaaagg atttatcgat gtagaaacgt tgaataaaaa acgaggaaaa gtatccagtg
     2221 tcggcgacat tttgaataaa catgtttttt acgtaaaaga atccgcctat cttcgtgata
     2281 ccttgcaacg cattttaaaa cgcgggctga aatacgtgcc ggtcgttgat gaacagaaca
     2341 aagtcgtagg tatcctgact cgtgcatcat tagtggatat cgtctacgat gtgatttggg
     2401 gagaagatcc cgcaactact gatgttgcag agtcagctac tcctgaagaa tctaaggagg
     2461 tctgacctat gcagcagttt tttcatcagc acggttcaga aattctctca aaaggttttg
     2521 aacatcttta tatttcagga cttgctttga tcattggtat catcattgct gtaccgcttg
     2581 gtgtcttact gacacgtttt ccaaaagctg ccacgattgt gattggctta accagtgcct
     2641 tgcagacaat cccttctctt gctttattgg cattgatgat cccgctattt ggcgtaggta
     2701 aattacctgc gattatcgca ctctttattt attcattatt gccgatttta cgtaatacgt
     2761 acattgggat gaaaaatgtc gattggaatt atcgggatgt ggcaaaaggc atggggatga
     2821 ctgagttcca atccattttt tccgttgaat tgcccattgc tatgccaacg atcatggctg
     2881 gtattcgttt agctgccgtt tacgtgattg catgggctac acttgcctct tatatcggcg
     2941 ctggtggttt aggcgatttg atttttagtg gactgaataa ttaccaacca gatctcattt
     3001 tggcaggtac gatccctgta acaattttag cactattggc tgattggctt ctaggtctct
     3061 tggaacaccg gctgacaccg atcgcactta gagaggagaa tgaagaatga aacgtatact
     3121 aaaaatgacc atggttcttt tctctcttct tatacttgcg agttgttcct tccctggtct
     3181 tgccagcaat acagatgaag acaccatttc gatcacaggt ggaatcactt cagaagcaca
     3241 gatcttagca agtctagttg ccggaatggt tgaacattat acagataaaa ataccgcaat
     3301 catcaacaat ctggctacta caactatcaa ccatcaagcg atgatgaacg gagatgcttc
     3361 aatttcagcg gctcgctaca caggaacaga tttaacgact acattgaacc ttcctcctga
     3421 aaaagatccg aaaaaagcgt ttgctaccgt gaaagatgaa tttgagaaaa gatacggtca
     3481 aacgtggttc ccgtcctatg gttttgaaaa tacgtatgtc tttcttgttc gaaaagacac
     3541 cgcacaaaaa taccatctat ctaaagtcag cgatctgaaa aatgtagcgg atgaattagt
     3601 agctggtgta gatacttctt ggatcaatcg taaaggagac ggttatgacg gcttccaaga
     3661 aacttatggc ttctccttca attcgatatt acctatgcag attggacttg tttatgacgc
     3721 agtcgaagca ggaaaaatgg atattgttct tggctactct acggatggac ggatcgccag
     3781 ttatgatcta gtcatgttgg aagatgatcg tcaattcttc ccaccatatg atgctgctcc
     3841 agttgtagac aacaagatat tagctgatac gcctcattta aaagaagcct taaataagtt
     3901 aggtcatacc atcagtacgg aaaaaatgca ggaattgaac tacgaagcag ataataatct
     3961 agtggaacca tcagtagtag cacaacgttt cttacaagaa aaccactatt ttgaagggaa
     4021 gtgaagcaat tggataaaat gaatgtcttt caacaatttt tctattattt ccaggaaaat
     4081 ggctcctata tctttgcaca atttatccgt cacttcctga tttctatcta tggtgtcctc
     4141 tttgcagcag ttgtcgggat tccggttggg atcatgattt ctaggagacg taaattagca
     4201 aactgggtga ttcgtttggc gaatatcatc cagacgatcc cttctctagc catgatctcc
     4261 attttaatca ttggattagg cctgggagtc aatgtcgtga ttgttactgt atttttgtat
     4321 tccctcttac cgatcatcaa gaatacgtat accgggatga tccaagtaga caaaaacatc
     4381 cttgatgtcg gcaaaggcat ggggatgact gctagacaac gactattcat ggtagagtta
     4441 ccactctctg tttctgttat catggcaggc ataagaaacg cgttagttgt tgccatcggg
     4501 ataaccgcga tcggtgcatt cgtcggtgct ggtggtcttg gagatatcat tatccgagga
     4561 accaatgcaa cagagggcac atcgattatt ttagcaggtg cattgccaac tgcattaatg
     4621 gcgatcatca cggactggct tttaggtatt ttggaaagac ggctagatcc agccagccgt
     4681 acatcacgct gatgttattg taaaaaacaa aagcttctgc gaccatttgt tggtcgcaaa
     4741 ggctttttta gttctgtcat ttttaaggca ataatccact ctgattcttc cctccttatg
     4801 ggcaaagagt tgcttttctt agctatttat ccattccgta tactccaact tcatactgcc
     4861 aacgggttca tatctgcatc caccgactaa gtttttttct tatccgctaa ggcattttgc
     4921 caatccattt tcccatcact cgtcatggaa ttcataccac atgatctcta aatactcgtc
     4981 tgcttgttct ttgaattggg catcaattct tacatccttt ggcgtggctt tcatttaagc
     5041 cttttccatc agtcctcgat tgttcgatgc cttcagctgc atcatcaaaa ctggtgactg
     5101 ccgtatcggt aacttcgatc atcttttata caatcgggaa cttgccatcc tcatccttgg
     5161 ctaaaaacat atctagccat tccagcttct cgccatggga ggtttgcggt tcctaaaacc
     5221 ggtacacgat tcttatggac tgcatccgtc gcaactagac ttggcgatac gatgatcctt
     5281 caccagaaga gcacccccgt aaaccaattg atcgatatat tgccaataag aaaagacatt
     5341 ggcgtaaaag atatcgatcc ctcgtgatgc attgccgttg atactgctat tcatggccga
     5401 aagcgccacg actttaactt cttccgtttg agtgacattc atgttcggca atgtcgcttt
     5461 ggatacccgc tctgctaaag gaacgacact ggtattgtat ttcgtatctg tatcttttcc
     5521 aaaagaccat gccaacgaat cctctgggaa ccaataagac gactccggtt gattgtccat
     5581 tgcttttttc aacatacttt tggctgtttc tcgataaacg acatttccaa ttgtcttcgc
     5641 ctcactttgg cgataatcga tggtcgttgc cactgccaac actaacaacg atgctccaat
     5701 tttactgatt ttccttgtca cacttcttcc tccttgtttt acataaaccc gtcaacattc
     5761 cattcctaaa ctcaaacaat gacccttcaa aggtggagat ataggaacaa attaaagaaa
     5821 tcatgcaata aatccaagaa ggttggtttt agaacaaaaa aagagtctcc tttttgggga
     5881 gactctttgt taactatcta aaaaggttgg ctcttctcac ttcactcata atgtgtccag
     5941 gcagaataaa tttctacaac tttattttcc tcatcaactt tgtaaaccac tcgatgctgt
     6001 ctattcagcc tgcgtgagta tctgccttca tgtgtaggtc tcagcttttc aaaagactgc
     6061 gtgggcatat atggatcttc cttcaatgtc tgaatcactt cttcaaattg tttctttaaa
     6121 ttcgtttgct tgatttttct taaatccact ttcgcagagt ttttgattgc gactgtatag
     6181 ttactcatca aagagcttcc caatcaatat catcgatatt cgtaaagcta ctgtcgtctt
     6241 tttcgcggtc tcgtacttta tctaaagtac ctgtttgctc aagaaaaagg gtttcttgaa
     6301 tcgctctcca gtcttccagc ccaatcaata cagcattgtt tccactacga tcactaataa
     6361 tctcgatttc agtatgattt tcatttacct ctttcaataa ctgataaaaa tttttacgag
     6421 cagtgctagg attcatcgtt gtattagcca ttgtctctcc tcctttttct gatataatta
     6481 tagcgtactt gattaagtac gtcaaaaaaa tacaataaag cacttaaatt cagcaaactt
     6541 ttcctctttt ttacatagag aaaaagaccc ctttttaggg aggatctttg tccacgctca
     6601 cttcttactc ctttacgact agcggtagcc aaacgtaaag taattggtaa caacgtatca
     6661 tctttagcaa aggtcgaatc caaagcctgt gagtctatgc aacaacacca atacacgaag
     6721 gttcctcgtt gcagtgtgac ttgattgata tcttgaataa cctgaggata cgcacgaaag
     6781 atttgtaacg gttattcaaa actgattttg gccgtcaagt cttctactgc ctcaaaaatg
     6841 attgcctctt tcgtctcctg tcattcatgt tcactatcga tttgatactt gcttagaccg
     6901 ttgggtttgt gaatcaccaa ttaaaaatgg cactgagtta tggtaacaaa aaatggtcac
     6961 ttgacgaggc tgagattcct acttatgaag acacatggca cttaagagtc gaggtaaagg
     7021 aagaagctga aaatatagga ggctgtacat tatttggcaa aggattcgga aattatgatc
     7081 aagacatcaa aatcaaaatg ttttacagct aaataggaag aataataaca tttcatacgc
     7141 tgttcccatc tttaatggct tatagagggc acctttagct gagaataaga caaaacagac
     7201 cctctagctt caattaagag ctagagggtc tgtttttgat tttaaaactt ggtttatttc
     7261 ttcaaatggt agctataggt gctgacaacg atattttcac gccaagacaa tcgcaatctt
     7321 aaccgcaaac ttgtttggtt atggagaata ttctgccagc gtctgtttgg tacaaattga
     7381 ggaatcaaca cagtagttgt atagttttgt ttcgctgcat ttttacttac gatatctaca
     7441 taacggataa ttggattttc aatcgagcga taagaagagt ggacgattga aaaacggaca
     7501 tctgggaaat gctttttaaa ttctgcttcg atttcttttt ctttttcgac atcttcgtct
     7561 agcgaaacat gcatcgcaac gacataatca ccgatcgaac gtgcgtaatt caacgcaccg
     7621 atatttaccc gtgtcacatt cccgactaat acaagaacgg tattcccgtc ataatcatgt
     7681 agttttgcat cattttctaa acgaagctgt tctgctactt ttttataatg atcatggatc
     7741 ttataaaaaa tgaacataac gattggcata atgatgaaga aaggccaaat gtcccctaac
     7801 cgatagacaa acaaaatcgc aatgattgca tatgagatga acgcacccgt aatattggca
     7861 atcgatttgc ttaaccatct ttttccttct tttttccatt tgacgaccat ccctgtttga
     7921 gataaagcaa atggaatgaa cacgccgata gagtataacg gaatcaagcg ttctgttgag
     7981 ccatggaaaa taaacagcaa tacgatcgaa cctagcgcta gtgtgataat cccattggaa
     8041 tagcctagtc gatccccacg atcttgatac atgtgaggca tgaatttatc ttttgccaga
     8101 ttgtatgcaa gaactggaaa agcagaaaaa ccggtgttag cagcaactgc taaaatcagc
     8161 gctgtagcaa attgaaggac gtaatacaaa ataccatgtc caaagacagc tttaccaatt
     8221 tgagacaaaa cagtgacttc tttttcagga acgatcccat accaatagtt gataaatgtg
     8281 atccccacga agaagaaacc taagatcaat gccatcatcg tcaatgtagc agcggcattt
     8341 tttgctcttg gcttcttaaa gaatggtaca gcattactaa tcgcctccac cccagttaaa
     8401 gaggaagaac cagaagaaaa tgctcgtaaa atcaaagcga tagaaacacc cggcacagcc
     8461 gctcctggaa gtgcagtagc ttgcaatggc acagcacctg tcacgatatt atataaacca
     8521 acaacgatca gtaacgagat cacaaggata aatgtgtaca ccggaaataa taggaacgat
     8581 gcagattccc tcagcccacg aagattcagc atcatcagta gcaaaacgat tgtcacagaa
     8641 atagcgactt gatgaccgta gagtgctggc accgctgatg tgatcgcttc agcacctgct
     8701 gagacagata ctgctactgt caacatgtag tcgatcaata aagagccacc ggaaatcaag
     8761 ccagcattct ttccaagatt ctcactgctg acgacatatg ctcccccgcc atgaggataa
     8821 gcatggatga tttgacgata tgatagcgtt aatgaaatca ataaaataat aacaaaagcg
     8881 gcaatgggta gcgaatacca gatggctgca gcagacaaag cgactaaaac gacaacgatt
     8941 tgttccgtac catatgcaat agatgataac gcatcagacg acaataatgc caaagcagca
     9001 aatcgggtta atttgtgctc atcattttca gcagatttta aaggcttacc tactaacaac
     9061 ctttttaagt aatccaattt ctaaactcct ttacacacaa ttttacttca agttttatag
     9121 tcgaaaattt atagtaaaca agatgttata gtacgctcac tgaccttaaa agtcaataaa
     9181 ctttaagaaa ataattggaa aaaaacagaa aaatatcaag cgctttttca aacgctttaa
     9241 tacagcaaaa atctaacatt tttttatggg ctttttcttt gaattctata ataaaaggat
     9301 agaacttgac ttagaaaatc aagttctatc ctttttgata tcttaacttt tggaagagag
     9361 atgtaaggct gctttctacc catcattctt actactcgaa gcatagcgca gttgtcacaa
     9421 cctcttgata aacggtgttt aaaagcttgc atctgcggta ataagcccaa ccaaattaaa
     9481 aacataagaa gctgttttta tttggtcgtt cttattactt gatgcagagc agctttttta
     9541 caacctcttg gtacacggtg ttcaatcagc tgaccctcgg tattagctca aaaaaacggc
     9601 aaaatatgag gaactatttt tccatttttt tctctaatac tcaggtcaaa acactgattt
     9661 cacaacctct ttttttaatg aaaaatatgg ctctcatgcg ccgcttcttt gaatttcgtc
     9721 attttttctt cgatgaacgg atagattgcc gttcctacga ttccaaagac aacaaacaaa
     9781 ccgatcatga tccagattgc tttcgtagca tttggccaat aaattcctcc tgcagattcc
     9841 cgtaacaagt ttaccgcatg agtaaatggc aagaacggat tgatcatttg gaagaatttc
     9901 cctgagactt ggatcggata gttcccgcct ccgccagaga tcgacagtac taagatgata
     9961 atagcgatcc ctttaccgac attatcgaat aatgcagcca agacatagac gatcatcatg
    10021 aacgctaagg cgatcaagac agcaaatgac acgctgtaga ccggattttg gacatctact
    10081 cctagtaaga aaatattccc taatgtaacg atcaagcttt gcgcaatcgc cattgtcaaa
    10141 aatgtcagca tacgagcaac gaattgttct cgtttcgtaa aattgtttcg gtctttcttt
    10201 cctaaatagt attctgtcgt agcaacactt gaaagaagaa cagcgccgac ccacagacat
    10261 agtgcagtat aaaacggggt gctagctgaa ccgttatttg cgattggata aagcgcattg
    10321 gtttgtagct caacaggttg tgtaaagaag tcactttctt ttgttgcgtc tagtttcaat
    10381 aatttgatga cttcgcctag atcgacttct tgttcgcctt ttcggatcgc gtctgctgct
    10441 ttttggatac cagctttgat atttggccag tcattgttga tcaagtccgc agcagcgttc
    10501 acagctgttt cgacttctgg cattttttca tttactgttg tcaatgtacc ggtgatttca
    10561 ttttttagtg ctggccaatc attttggata aaatcagcgg ccaatcctaa tttttgctga
    10621 atgatcggta attcattgtt gtataggtct gccccttcat tgatgccatt gacgatggta
    10681 tccatatttc cattcagcat tgtattggca tcatggattt cttgtccaat tgcaggcatt
    10741 tctttttgat atttttctaa aattgccact gcattcgtta cagttgcttt cgttgagctg
    10801 agaagcgaat caaagtcgat ctggtccgct ttgctcagtg cgccttgtgc agtcccaatc
    10861 gtatcgatga cttggtctag tagagaagtg acagtggaac tgatttgacc tgtattgata
    10921 ccagcaatga tgccattgat attattaaca acggcatcaa tttgatctag caattcatca
    10981 atagccttcg tgtcttcatt ttgaacagct gtgttcaaag cagataggcg gttttgcaga
    11041 ttgcttagca agggttttaa ttgatttagc tgatcaataa ttggctgtaa tttcgtattt
    11101 ccagcacttg tttgcaattt agtcaggaac tcgaccagtt tatcgattgc attttgttgt
    11161 tgagatattc cgtcaataaa gccgttcaat acattgctga tgtttttctt ttcatcatca
    11221 gtcagcttac catcttcaat agctgttctt acagtatcta ttaacgaacc tgtcgttgtt
    11281 gccacttgtc caattgactt caatgtaaca tctacactgt ttgtgatgct tggtatcgca
    11341 tctttcagct gagaggcacc tgattttgtt tggtttgcta gatcacttgc ctgattttct
    11401 aaatttttca catctggcaa gatatcttgt acttgttgga tgatcgtcaa gccttgtttt
    11461 gcttcatcga ttccttggtt cattgtctct tggatcgaag caaaatcgcc gtcgatctct
    11521 gctagttgct taccggcatt ttggatctct gggatttttt cttgcaaatc aaggatgatt
    11581 tttgcttgat ctttcaactc aggcattttg tcattcaatg caacgacttt ttcacccatc
    11641 tcatctactt tagggatgta atccacgaat tcatttgctt tattcagttt ttccttgatt
    11701 tcaggtagtt gactttgtaa ttcgaggact tgttttgtgt agccgtcgat cgtgtccaaa
    11761 ttttcatctg ttgataaaat catatcttta actttattga tacttactag gttcgtatcg
    11821 atgtcgtaac ctatctcatt gaatactttc aataaagtcg aactggctgt tttgataaat
    11881 tcatttgtga tttgcgattg gatcgaactt gctcctttat cggtgatttt cggtgcaatc
    11941 gcattgattt tttcatttac cgtatattcg atttttggtt tcttgatatc accactagta
    12001 aaactcagca gatcttctga gaaatccttt ggcaagtaaa tacctgcgta atactttccg
    12061 gaacggactc cgtcttctaa ttgttctttt gaatcaacga attgccatcc taattgttta
    12121 tttttatgaa gcgattcgat gacttgctcc ccaatggcca cttctttacc ttgaaactct
    12181 gctggcttgt ctgcactgta aacagcaatt ggcagctctc cagtatttcc atatggatcc
    12241 cataatgctt tgatgttaaa ccacgcatat agcgaaggga tgatcatgat cgcgatgatc
    12301 agaagtgttg caatcgggtt tttgaaaatc cgtttccagt caagcaaata taatcgaagc
    12361 gtatttttga ttgactgcat agttctcccc cttcttcttt tttcagctga attactttac
    12421 catgtatttc atactttttt caatcggctt ttttcaaaaa agtcgaattt ttggacatat
    12481 cacggcaatt gtctcattgt aatctcttac atattgagaa ataattaagg attacagcct
    12541 ttactttatc atgaaataaa agggaaagat aattttatgc caaagttttt atctattata
    12601 cgtatacgca aaaaagctaa aacatttttt gtctttgccc ctacaaaaat gtttcagctt
    12661 ttttcgtttt ttattcctgt atttcttatt tttcgaatga caggatgatt ttcccagcag
    12721 gaggatgtcc ttcaactagc tgatgtgctt ggatgacatg cttaagtgaa gcagacagat
    12781 tcattgaaat aaaaacatga aaggctcctt tttggtaggc tttgattatg ccagcaaagg
    12841 cttctgcatc tagatattcc tttcttggaa caaacgattc ataaaagcct tccttttttt
    12901 gccgcaaatc tagatctggg aggtcattta atgcaacata cctgccgcct ggtttcatga
    12961 tctggatgcc ggtttctcct ttgatacttc ctttcgtcgc atcgattaca aggtctgcct
    13021 gatcagcaaa ttgcagacct ggatttgttt tatcataagc agcaaatgct gaagctccta
    13081 atttttttac cttttcctca tttttactgg atgcactggt taagattcgg attccctttt
    13141 catggagaag ctggatcaga cttgacccga ccgcacctga tgctcctagt atcattacag
    13201 tatctgtcgg ctggatttcc gtcaaatggt tgattagatt ataagccgta attcctggcg
    13261 tgaccattcc ggctgcttct tcccaactca ttttatctgg aatttttgct acttttgctg
    13321 atggcaacac gacttcttct ccgtaagtcc cgccgactgc atggacagct actcgatcac
    13381 ctacatgaac agttgtgaca tctgaaccaa tctctgtcac aataccagcg ccatcattac
    13441 ctggtacata agggaatttc aatgttcgta tctctttcat ttctcctaaa cgcaaggcaa
    13501 catcataagg attcacactg aatgctttta tttctacacg aacatgtcca gaaccgactt
    13561 ctctgggatg agcatcgatt tcttcaaaaa catcttgtgc ttctccgtaa ccgttaattg
    13621 caaatcttcg catggtcacc atctcccttt ttctttaagt ttacgtttat gcctttattt
    13681 tgacaaaaaa aatgtctttt cgttttggcg catgattcaa aacaaaatag ttgtcttttt
    13741 tcgcgtaacg tgttaaatta tttatctaaa agcgtaaaga ttacgattta cgctaataaa
    13801 aaataattgg aggaatttgt tgatggctaa aaaatcaaag attgccaaag caaaaaaaca
    13861 gcaagaaacg attgaacgct atgctgcttt acgccaggaa ttaaaagcgc aaaaagatta
    13921 cgaaggcttg cgtaaacttc ctttagatgc tcgtccagaa cgattgaaaa atcgtgattt
    13981 aatcgatgga cgccctcgcg gatacatgcg taaattcggg atgtcccgta taaaattccg
    14041 tgaattagca cataaaggtt tgattccagg agtaaaaaaa gccagctggt aatccaagtc
    14101 tggctgtaat gtaaaggagg agggcgcatg gaccactcct tggatcatca aaaaagatac
    14161 gttcgcttct tctagcagaa atgctggtac tagaagcaga cgtatctttt tttacgcaca
    14221 gctggtcctt ttttatatgt aaaggctgca tggttacttc gctatattta tttagtaaca
    14281 ggagtcagtg cttcgatacc cacactaatt ttcaatgctc ggtcaacttc tgccataatg
    14341 ggtcgatcca aatggcagac cttttctttc aatcttgatt tatcgatcgt tctgatctgc
    14401 tccaacagga taacggagtc tctttctatt cctgttccag tagctttgat cccaatgtga
    14461 gtcggaagct taggtttagc catttttgcc gttatggccg caacaatgat cgtcgggcta
    14521 aaatggttac ctagattatt ttgaataacc aaaactgggc gtgttccgcc ttgctccgat
    14581 cccacgactg gagaaagatc agcaaaataa atatctcctc tttttaccat aggatcctcc
    14641 ttattcttga tattctcttg gtatccgctg tcctaatccg caagccactt catagtggat
    14701 cgtacctaat tgatctgcta tatcctgcat agtgatttct ttgtctgcat ttttgccgat
    14761 aagcgtcact tttgttccga caggaaattc ttgaggcaat cggatcatca gttgatccat
    14821 acatacacgc ccaatcgttt cacagtagtt cccttctacc aatagagaaa aaccctgcat
    14881 ctttcgtggc cagccatctg cataaccgat cggtatcgtt ccgatccact ctgcttcagg
    14941 agtgatatat gtttctccat agccgattcc ttcacctgct gggagttttt tgacttgaat
    15001 caattcagaa accagctcaa gtgctggctg caatggataa acttccggta gtgcgtgccc
    15061 agagggatta agtccgtaca ttgctactcc ataacgaatc atattgccga ttgtttcatc
    15121 atgccataaa gccgttgcac tattgctgac atgtacatat cgaggaagtt cgggaagttt
    15181 tttcagcact tctataaagc gttctttttg cagattccaa tatgtatcat ctgcctgatc
    15241 tgctgtagaa aaatgggtaa ataaaccttc ccattctaat acacgacttt ctttgatgaa
    15301 tgcagctgcc tcttttactt cttcaggtgt acaaaatccg atacgaccca tcccagtgtc
    15361 tgcttttaga tgaatcgaaa gaggcgtctc tagcttactt ttctctaaaa cagcagctgc
    15421 tgcttgcaac cattcccgtg tacctgcagt tactgataaa tcatgagcaa cagctacagt
    15481 cagtgcgtcg taaggaacca tattcaagat aaggatgggc tgagtaaatc cagcttctct
    15541 caactcgact ccttcatcta aatttgaaac acaaaagccc gaagctccac ctgcaactgc
    15601 tgcctcagct gtttcaatag ctccatgacc gtatccgttc gcttttacta ctgcaaacaa
    15661 ttcttttcct tgcggaagtc ttgcaacttc atttgctaca ttttcagtta tagctttttt
    15721 atgtatcact gctttcgttg ggcggtgcca agcgacaacc atcgtttttc cttctttcta
    15781 gtcttccaaa ataatttgtg caaaagctgt ttcatttgta tgagtaatac tgacccatac
    15841 attgccttca tgaggtgaac gcgtcacaac aggctgacca ttttcatttt ttaaaatctc
    15901 gacatcttga aaggtaactt ttccgatccc agttccccaa gctttagaga aagcctcttt
    15961 gcaagcgtaa cgcccgccta aaaattctac ttggcgatga aaaggcaagg actggaatag
    16021 tttcatttca tcagaggtca agattcgtgc aatgaatttt ggtttttctt cgatcagccg
    16081 agtgatccta ggaagttcga ctgcatcgat cccaattcct tttatcatat gcttttctct
    16141 tttcaataaa tttagaacac tctcattcta ccaaattata accattttgc cacaaataag
    16201 tccacaaata acaaaaatga aagtttctct taaaaaagag aaactttcat tttatgttta
    16261 atcgttattt gaacggatta caaatccgcg ttttttatca ttgtgacgtt taccgccatc
    16321 tttttggctg ttattatttt tgctataacc gccttttgat ttgtttccac gataattacc
    16381 gccgtcacga cgattacggt tgtttccgcc accacgtttg ccatttttat tgtatccttt
    16441 tttctgcatt ggcaatgggc gttctggtgt gatcttcact ggcactgcat cagctggatc
    16501 ttttgctgtc gtttttagca aaagtgcaac taaatcttgt gcagaatatt cttctaataa
    16561 tttatctgct gtttgcaggt atttatctag accattttct gcaagttttg tttcgatttg
    16621 ttcaactgca gcacctaatt gacctttgaa tgcttctttt tcggttggtg gacgtaaagt
    16681 cgtcatccgt tttttcgtca agttttcaat cacatgcaag tagtccattt catttggtgt
    16741 aacgaaagtc acagacatac cgccttttcc ggcacggcct gtacgtccga tacggtgaac
    16801 gtagctttct ggatcttgag ggatatcata gttgtacaca tgagtgacac ctgaaatatc
    16861 taatccacgt gcagcaacgt ctgtcgctac aaggatatct aaatgaccgc tcttaaatga
    16921 acgcaatacg ctcattcgtt tttgttgaga caaatcgcca tggattcctt ctgcacggta
    16981 accgcgtgct tctaatccgc gtgccaattc gtctacacgg cgttttgtac gtccgaagac
    17041 gattgttaat tcaggtgttt gaacatcaaa caagcgagtc atgatatcga atttttcgta
    17101 ttcttttgcg cggacatagt attgatcgat caagtcagct gtcatttctt tcgctttgat
    17161 cttcacatga tgtggatttt tcatgaattt aacgccaata ttcttgatag ctggcggcat
    17221 cgttgctgag aaaagtaatg tttgtctttg atcaggaact tgtgaaatga ttttttcgat
    17281 atcttccaag aagcccatat tcaacatttc atctgcttca tccaaaacta atgtttgaac
    17341 ggttccaagt ttcaatgtgt ggcggttaat gtggtcaagc atacgtcctg gtgtacccac
    17401 aacgatatgt ggacgatctt tcaagccacg gatctgacgg ccaatatctg ctccgccgta
    17461 aacggcttgt acacgtactt ttttgtcgcg ccctaaacga tataattctt cttgtgtttg
    17521 gatagctaat tcacgtgttg gtgcgataac taaaccttgt aattcgtgac gatctggatc
    17581 gattttttct aacattggta atccaaatgc agctgttttc cctgtacctg tctgtgcttg
    17641 tccaataacg tcttttccag ctaatgctag tggaatcgtt tctgcttgga taggcgttgc
    17701 ttcttcaaaa ccagcgcgtt cgactgattt caacaattct ggtgataatt ctagttcttt
    17761 aaatttcaaa tgaatcctcc tatatggttg ttaactgagg atgtagcttt tttgctaccc
    17821 cgtcgatttg ttcggtcaaa gtgtattgtt tctttttgac agtggccacg cacgttttta
    17881 tccattctta gcccctgtgg caccaccgaa aaatcacttt gcgaagttta ggcattagaa
    17941 aagccaaacg ggtcgcgttc agctttatca ttattatgac atctggcttc aacaagttgt
    18001 ttctatggca aaaccagtcc aatttctctt catctttcgg caataggtta ttctaacata
    18061 gtttttcgtt ttctgcaagt ttacagattt ttatttcatt tgctgaagtt gttctaccac
    18121 ttcaaataag cccatgctgt tgctgccttt tagcataata ctatctgttg cttcaagatt
    18181 ttgtttgata gcatcaatca atttattctt ttctttttcc aaatagaaca caggtagttg
    18241 ggggtatttt tggttcaatt cttcttttag tgcatgcatt tgttccccat aaaggaaaat
    18301 catggcgaat ttctcaggat cgatatgttc actcatttgc gcatgcatat ttaaagactc
    18361 tttccctaat tccagcatat ctgctaacac agcgattctg cgtccttttg ttggcagatt
    18421 tttaaaagta tctaatacca gtcccatggc tgttggattt gcattgtaga catcgctcaa
    18481 aatagctgca ccatttcctg ccgttagcca ttcggttctg ttttgagtga tagaaacatg
    18541 ggcgagccct tggcgtatct caggtatgga cagtccaaag aagcgcccga tcgtatacgc
    18601 aatcaaagca ttcgttacat tataacttcc aggcagcggg atcgtaaatg tttctccttc
    18661 tactacaaaa tcggtttgtg cttttccttt tgctgtcact tccgcatgaa catcgccatt
    18721 tccaataccg aatgttgtga ctgtttgtac taatgttgcc gttaaaggtg tcagcaacgg
    18781 ttcatctgcg ggcacaacga gtaatccgtc tgatacaagt ccgtcagtga tttccatttt
    18841 ggctttagcg attccttctc gcgacccaag attttctatg tgtgcttcac cgatcattgt
    18901 gattgctgca gcttttggtt gagccataag tgaaagttcc gtgatttcat tggcgtgatc
    18961 catgcccatt tccaaaacga tcttttctgt atcttggggc atgtgcaaaa tcgtgtaagg
    19021 aagaccaata ttattgttgt aatttccttg cgtcttatat gttttgtatt tttcagacaa
    19081 gacagacgcg gttagatctt tcgtggtggt tttaccattg cttcctgtaa ttgcaacgac
    19141 ttctgcgcct actttttcca agtaataggt tgccaattgc tgcatggctt tcaaaggatc
    19201 tgctacagga ataactggaa aattttttgg agcttgttca acaggcaagc tccaaaatga
    19261 agcagatgct ccgtttgtga tcgcttgctg gataaattca tgtccatctc gcgtaccagc
    19321 aagaggaacg aataaacttc cttcggttat cttccggctg tcaaattcga cattggtaat
    19381 ctttgtcagt gcagagcatt cgctgcccac ggcttgtgca atttcttgta ttgttagatt
    19441 cattgagatc tctcctaaaa aaggttgtga aaaaagcgtt tggcctaaac ggcgggttcg
    19501 catgactctc acttccaagt aaatttctct attcgtattt agaagctaga atgaacgcgg
    19561 ttacgcaatc actccagcct cttctttatt tctctttatt catttttttc aaaaaaagat
    19621 tgacgctgat ggtatcgatt cattcctaac tggatcagtt cttcaatcaa atcaccgtat
    19681 ttcaagccca tattttccca taagagtggg tacatactga actccgtaaa tcctggcata
    19741 gagtttaatt cattcaggaa taattcattt ttatttgtca aaaagaaatc gcaccggctc
    19801 aatccgcttc cacctaacat cgtgtaagct aacttcgcgt actcttgcgc tttttgataa
    19861 acttcttctg gcacttcggc tggaatctgc atttcgattt tattattgat atattttgct
    19921 tcataatcat agaatgctac gtcttttacg acttcaccag gcaaagtcgt ccgaacgtct
    19981 tcatttccta atacagcaac ttcgatttcg cgcgcttcaa ttccttgttc aacgattgct
    20041 cgagaatcat actgataggc tgttgctaaa gcattttgca gctcttctcg gttttctgct
    20101 tttgtaatgc cgacactaga acccatattc gccggtttga caaacatcgg ataaagcaaa
    20161 gaaccttcac attgatcaaa tactttttta ggattttctt tccattgatt cttaagtact
    20221 ggtacataag gaacttgcgg cacaccagca gcttgtaaaa tatacttggt catgatttta
    20281 tccatggcac atgcactggt caatacgcct gcgccgacat aaggcatatt caatgtctct
    20341 aagaagcctt ggatcgttcc atcttccccg tttggcccat gtaaaactgg aaaaacgatg
    20401 gctccttctt ctttgatttc gcccggattg atcacttttc ctgtaaagcc ttcctctgtc
    20461 tgtccacttg ggtcccatga aagatgcaag acatctttgc tggcaggttt ttctgttagt
    20521 aatggacctt tgacccattg tccttctttt gtaataaata cgagttgaac ttggtaataa
    20581 ttataataaa tggcatttaa aactgaaaat gcggaaagaa tggacacttc atgctctgcg
    20641 ctgcgtccgc catatagtaa agtaatcttc aaaagattgt cctcctgtat tctaaatcgt
    20701 tcagtgattt actctcatat tttagcacaa aacaaaaata aacgaataac ttatttataa
    20761 aaaaacgagc cagcttctaa aagaacgagc gtcatttatt ttttgtcagc tagattattt
    20821 gtcccgtttt ttataaaaag ttatgataaa gaaaaggagg gatgatgatg gcgcaaacaa
    20881 atccacgcag ccgcttggca gaaatcattc gagtgttcat caattacaat gtagtgccaa
    20941 actttgtaca gcaaaaaaat cccgaacagg taaaaaaagc ctttgaagaa ctaggcccca
    21001 ccttcatcaa gataggacag atgctttctg ttcgagaaga tttgctttct tctgccttca
    21061 ctcaaacatt caaaacatta caagatagtg tacctagcga cacattttca acggtcaaaa
    21121 agacgatcga aacagaactt tctctttcat tatcagatat attcgatgat ttttcaaaat
    21181 cgccttttgc ctctgcttct atggggcagg ctcatcgtgc caagctgaag aacggtgacg
    21241 cggtagtcgt taagatccaa caccctaaca tcgccgaaga gatccgactt gatcttcaac
    21301 tatttgaacg agcgatccct ttgatcaaat acattcctga aactagcgta gttgatctaa
    21361 aaggggtact ccaagaagta aaacgttcct tgatcaatga aatggacttc ttaaaagaaa
    21421 gtcaaaatgg cgaacaattc tatcaaaaga acaatggctg gaaagagatc cgcagcccca
    21481 agatttatga cgctttttgt tcgaaaaaag tgatcgtgat ggaagaaatg tctggtaaaa
    21541 acttgaatca tttaatgaac gccgaaaaca aaactgaaac ttttatcact ggaattcaaa
    21601 acaagcagct gaaacaagaa gttgcaaagc ttttagttga gaactttatg aaacaggtgt
    21661 ttgatgatgg ttttttccat gctgatcctc atccagggaa tctgctattc catgtactaa
    21721 caaaagaaga acagacacaa gctagccgaa aaactgaaac agtccatgaa aaagagttcg
    21781 gttcctttgc ttttcgagcc tccacttctg cagaagaccc tgttgcgcca tataccatca
    21841 actacatcga ttttggtatg atggggcatc tttctgcagg acttaggcaa aaactcacac
    21901 aagccgtttt agcgctgtat acgaaagatg cttatcgaat cgaaaaagcg gtccttcgac
    21961 tttgtcagca agaaggctcg tttgatgaaa gtcggttcca ccaagaactg acaagttttc
    22021 ttgaacagta ttatgattct cctattgatg aaatcaactt gcaagaagta ttcacacatg
    22081 tcgtgacgat ttgtcatcaa aataatctgc agttcgatcg tgatatcacg cttttgctga
    22141 aagcatttgg gacattagaa ggggtgatcc gcgtcctcga tcctgaagtt tccctcatgg
    22201 aagtcgcttc gccttttgct caacattatt ttctcactca tctggatgta gaagatacat
    22261 tgaaacaaag cggattagac ttattggaag ggatgaaggc tgcgcctaag atccctcaac
    22321 agctccacca tctattagaa atgtggactt cgggacaagg aaaagtcaat ttggaattga
    22381 aaaagcaaga caaattattg agcaggatcg aaagtatgat caatcggctt gttttcggta
    22441 tgattttagc tgcattgatc gtagggtcct ccctgcttgt ccaagcagca cctgtagaaa
    22501 acgcagaggt cgtttcctta ttagggatat ttacgtatgc tattgccgca ttcgtcatta
    22561 tttttttggc aatcgatgca ttgatccaaa tgtataagaa gaggaaaaag tagaaaaagg
    22621 ttgtgacaga aagaatcagc ttcaagaaat aagaaggggt tttacataaa ttgtttctca
    22681 aatttttgtg aaaattcggc ttatttttcg aagaggctac ttctgaaaca ccgagaataa
    22741 aaaatgtgaa aaagctgttc tgcacccagt aataagaaca atcaaaaagg gaacatggaa
    22801 cacatttatc ctgtttccct gttccctttt tgatgtattc gagcataaat aaaatacctt
    22861 cgttacctac tatcttctgt acttggtaaa ccttagtcaa ttgccttttt taacgcttga
    22921 cgaaaagaca tatgatcttt ctccgtaaag ggttttggac cctttgtcct tatgccagct
    22981 tttcttaact ttccactatg ataacgttgt tctagaagcg tgccgatatt cgcttcgtca
    23041 aattcttttc cagagtgatg gaacactcgc acacccttcg gcaatacaag tgaagctaaa
    23101 ccataatctt gcgtgatcaa aaagtcattc ttcttaacca taccgacgat tttataatcc
    23161 gcactatcag ctccgcggtc tacgtagacg atttggacat gattagcgta ttctttggtc
    23221 gtataatggt ccacactggt cacgataacc acgtcaagac cataggattc tcctaatttt
    23281 atgacatctt cttttactgg agaaccatct ccatcaatga atagcctcat tctttttcgc
    23341 cttcttccat aaatgataaa cagttactaa tccgctcagt aaaagaccaa cattgatcca
    23401 ttttgccaag ataagcagtt gtacactttc tatcggcact gattcatggt tcagcgtgat
    23461 caataataga tggattggct ctcccatttc ttttgctaaa acaaacaaaa gcgtcatcaa
    23521 taaaaactgg tggatcacac tccctttatt ttgagtagta tacttgacaa cgatccggcg
    23581 atccattgga atagctgtat cagtttgata atattcctta tttagccagc tgcctgttac
    23641 tacgtgccag aaactaagca gaaagacacc tatcgcaaaa ccatttggaa tcaagaggac
    23701 caataaagaa gaactcagtc caagcaattg catagccaat ggagaccatg ttaaacgttt
    23761 caaaatccat ggtccgacta tttttgctgc aatcaagcct gctgcataag gcaaatacat
    23821 aaaaatcgaa agtttttcta atccgtacag tatgccaaca taaaaactgc taaataaaaa
    23881 aaggaaattc cctagcattc catttagaaa agtaaacaga tttagccagc ttggcaactt
    23941 ccattccttc ttctggtgtc ctaaaacaaa cacgaacaag atgaacagca gcaagaaacc
    24001 ataaatagca agatcaaaaa gcctttcatc gaataacaat cgggcatttc tcaaaagaaa
    24061 caataagcca aagaatatca aaaataaaac aagctctcgc gtagctacca gatttctttt
    24121 gatgtctttg aaatccagtt cgtaacgcgg ataatgacta accgtatggt aggccatgac
    24181 gtaaaaaagt gtataaatag taaagcttgc tgcaatctga gtcggtgcag gcaactcaat
    24241 cgcttgatac aggagaaata gccagatagc tgcaaataag tactttttcc catccatatt
    24301 gataaaacct tgcgtctttt cgtgaaaatt gaccgtggta ttagcaggta caagccatgc
    24361 tgcactcagc cctagcaaaa cacttgaaaa ataaaataaa ggaaaataaa atacgcctaa
    24421 tattccaagc aatgaaccag caccaccaat cagcagagcg atcatcagca acgtgtaact
    24481 atctagacca aatcgtagac tgttgactaa aaatagtcca gtcatccgaa aggcataaaa
    24541 cagcgtaaat ggcaaaatcg tcagccaatt atgcgagtct gttagtccca aaaataaaag
    24601 ataaggaata aaaataaccg tgttacttaa gataaagggc gttgtttgtg taaaattacg
    24661 cccaaatcgt ttgagttttt cgaacatgac aatggctcct taaacagtct tataactaat
    24721 ctgctcttcg attttagcat taaaggggaa ataaaccaat tttacagaat atattcttgt
    24781 aaaaaaaaat acagaaagaa gcataagctt ctttctgcat ttttacggta cttgattact
    24841 caagcagaca agcccttctc cacaaaagtc ttgcccgaca cccctgcatc cggcaggtga
    24901 taaagataga aatcaattat cctgtgcctc caaaatcaat taggagctct gtaaaccaga
    24961 gtccttctcg acccgtcgca taaatttact ttaacacagt tcctatgttt atgcgagagc
    25021 ttttttcatt ttataatcga ccatttgaaa tccttgaccg atatctgaaa taagttcttc
    25081 tgtaatctca aatccttcat gcttataaac ctcgattgct tgatgattcc cctgattcac
    25141 tcgcaaaaag atctctcttt cctttgtttg cgcagcaagt tcttcatacc agcgaaaaac
    25201 tgaagaagtc aatccttgac cacgtacttt atttagcaaa tataatttac ttaaataaat
    25261 ttttccattt atctcttcat aagcagtgta accgacagtc ttttcttcgc tttttaataa
    25321 aaagtaggac acaccttctg tcagttcttt ttgaatctgt ttttttgact gataagtttt
    25381 cagcatgtaa tccacttgtt tttgaccaat gatcggcaca aaagcttccg gccatacttc
    25441 ttgtatgact gccatcagct catccagttc attttctgtt acttttattt tttccataca
    25501 cccacgtcct tttttacatt ataaatgacc ttgcgcttga tggaataaaa agagcatgat
    25561 catgctatac acacccaccg accatggttt caagaaaatc acaatctgca taaattccct
    25621 aaaagataat ttcgtcaaac cagctactag acagatcaag tcgtctggcg caaagggaag
    25681 cagcaaggta acaactagta cttttttaat atctttcgtt tttcttgtca gcaattcatc
    25741 aaatttctga tacttttttg gcgataaaat aagctggaca aaggctacgc catagtaacg
    25801 gacaagcaaa aatccgattg cttccccaat cactaggcca atacaactat ataaaagccc
    25861 aaatccattg ccaaacatca gcatgcccgc tactgaggag atccctcccg gcaaaatagg
    25921 aatgatcact tggatcactt gcaacagaac aaatatcatc actgcatact tgccaaattg
    25981 ctggataaac gcttgcaaag acgtgctcga tcgaaaaatc ccatgatgat aagcataaag
    26041 aatcaacaca aagaaaaaaa acagaccaat aatgggcatg atctgaagac atttttgtaa
    26101 aactgatcct tttttagaca tctatattcc ccacatcttt tttcatttat taaaactgcc
    26161 tcct
//
LOCUS       DAJSKS010000024        25689 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJSKS010000024 DAJSKS010000000
VERSION     DAJSKS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.024"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..626
                     /locus_tag="ON396_000876"
     CDS             <1..626
                     /locus_tag="ON396_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113003.1"
                     /translation="QRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWLLLD
                     HSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSFLAI
                     LFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSLFSF
                     FVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(760..885)
                     /locus_tag="ON396_000877"
     CDS             complement(760..885)
                     /locus_tag="ON396_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3113004.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1019..1912)
                     /locus_tag="ON396_000878"
     CDS             complement(1019..1912)
                     /locus_tag="ON396_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HCR3113005.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2094..3020)
                     /locus_tag="ON396_000879"
     CDS             complement(2094..3020)
                     /locus_tag="ON396_000879"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCR3113006.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3105..3866)
                     /gene="pflA"
                     /locus_tag="ON396_000880"
     CDS             complement(3105..3866)
                     /gene="pflA"
                     /locus_tag="ON396_000880"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCR3113007.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4110..6341)
                     /gene="pflB"
                     /locus_tag="ON396_000881"
     CDS             complement(4110..6341)
                     /gene="pflB"
                     /locus_tag="ON396_000881"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR3113008.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6618..9068)
                     /gene="parC"
                     /locus_tag="ON396_000882"
     CDS             complement(6618..9068)
                     /gene="parC"
                     /locus_tag="ON396_000882"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR3113009.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9080..11125)
                     /gene="parE"
                     /locus_tag="ON396_000883"
     CDS             complement(9080..11125)
                     /gene="parE"
                     /locus_tag="ON396_000883"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR3113010.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11291..11725)
                     /locus_tag="ON396_000884"
     CDS             complement(11291..11725)
                     /locus_tag="ON396_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR3113011.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11852..12490
                     /gene="plsY"
                     /locus_tag="ON396_000885"
     CDS             11852..12490
                     /gene="plsY"
                     /locus_tag="ON396_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR3113012.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12622..13497)
                     /locus_tag="ON396_000886"
     CDS             complement(12622..13497)
                     /locus_tag="ON396_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCR3113013.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13579..14370)
                     /gene="codY"
                     /locus_tag="ON396_000887"
     CDS             complement(13579..14370)
                     /gene="codY"
                     /locus_tag="ON396_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCR3113014.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14388..15788)
                     /gene="hslU"
                     /locus_tag="ON396_000888"
     CDS             complement(14388..15788)
                     /gene="hslU"
                     /locus_tag="ON396_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCR3113015.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15802..16350)
                     /gene="hslV"
                     /locus_tag="ON396_000889"
     CDS             complement(15802..16350)
                     /gene="hslV"
                     /locus_tag="ON396_000889"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HCR3113016.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16387..17292)
                     /gene="xerC"
                     /locus_tag="ON396_000890"
     CDS             complement(16387..17292)
                     /gene="xerC"
                     /locus_tag="ON396_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR3113017.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17490..18866)
                     /gene="trmFO"
                     /locus_tag="ON396_000891"
     CDS             complement(17490..18866)
                     /gene="trmFO"
                     /locus_tag="ON396_000891"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HCR3113018.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18835..20913)
                     /gene="topA"
                     /locus_tag="ON396_000892"
     CDS             complement(18835..20913)
                     /gene="topA"
                     /locus_tag="ON396_000892"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR3113019.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21035..21892)
                     /gene="dprA"
                     /locus_tag="ON396_000893"
     CDS             complement(21035..21892)
                     /gene="dprA"
                     /locus_tag="ON396_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR3113020.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21948..22715)
                     /locus_tag="ON396_000894"
     CDS             complement(21948..22715)
                     /locus_tag="ON396_000894"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR3113021.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22708..23568)
                     /gene="ylqF"
                     /locus_tag="ON396_000895"
     CDS             complement(22708..23568)
                     /gene="ylqF"
                     /locus_tag="ON396_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCR3113022.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23828..24226
                     /locus_tag="ON396_000896"
     CDS             23828..24226
                     /locus_tag="ON396_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3113023.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24505..25005
                     /locus_tag="ON396_000897"
     CDS             24505..25005
                     /locus_tag="ON396_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCR3113024.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(25088..25639)
                     /gene="lepB"
                     /locus_tag="ON396_000898"
     CDS             complement(25088..25639)
                     /gene="lepB"
                     /locus_tag="ON396_000898"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR3113025.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
BASE COUNT         6769 a         5449 c         4419 g         9052 t
ORIGIN      
        1 ttcaacgtag aaccactaat tttttacatt taggctcttt ttttgcaact ttgacgatct
       61 tattttttct atgttattca taccttaaga cctcacatgc gccttctagt gcattaggct
      121 ggttgctttt ggatcattct gatatcacag aagccgaaga aaatccgttt tttttgattt
      181 tgtcttctct ttgcggctta ctgttttctg tttatttcgg tgcctatttt tttcatcgac
      241 atcctggtac gttaaacgaa gcttcaagcg tattgattgc atttaaaacg ttttcttttt
      301 tagcaattct tttttattcg tttaatcaca atacgctttt cttctttatc atgaatggtt
      361 tagtcgtgat tttatccatt tttacttatt ttattttttc actcattttt agccaattga
      421 aatgtccgct cttctttcga ttagttcctg tcacctgttt tatttacagc ttgttttcct
      481 tttttgtcct tgctttaatg ccactgacca acccttcgac gatccaagtc gcaggaaatc
      541 tgttggatat gctcttcatc gtcagtctag gtgtatttat gtggcaaaga aaaaacaaac
      601 aaaataaaaa gcattttgaa gcctgactat tacgacttca taaatgcttt tttagtttta
      661 aacacttcaa tctaaaagaa taacccaaga ttggataaat tattaatgac tcgccattcg
      721 tttcttagtg attctggatt atcaaatgga cttcctaatc taatcagcta gttcttccca
      781 taaaatgcca tacttgactg aatcataata ttgattattt aaaaatctaa cttttcttat
      841 cactacttcc tttgtcattc cacatttctc accatttttt tgcattcctt tgtttccaga
      901 ccatgtagtg aatctaatag gtggtagata ggcaaaatac tcaaataatt cttttaacca
      961 taaaggaaga gaaaggctcc tcctgcaaga gtcagtataa tttttgctta ctgctctctt
     1021 aggttttact cactaaaact gttccatatt ctttgatata agaaaatgca tgcggtataa
     1081 atttcttgct cagctcagaa aagatttgtc gtttaagaaa ggccatttcc tccattgtca
     1141 atgaaggatc caatgttggt aattcaatct caaaattgtt tttttcgaca ataggtaatt
     1201 ctaatgtgaa tgattcttct tcctttatag tattcgtcac aagtagagac tgatggaaaa
     1261 tactaggccg gcgattttta tatttattct tacgtatcct gtcaacgata acttctattt
     1321 gatcaaaaaa gtacgattca tttacgtctc caataagttc ggctaattct ttgaatgcct
     1381 tcgttacagg aagctgattc ttctgtacaa aaaagtaatt agccaaagta agcgggaagt
     1441 gttctataga ggaaagtgtg ataaaacggt acttctcatt tcctgcagtt gtcctcagca
     1501 attctgtctc tcggctgttt cttactccat aaaaatacgc ttctttccgt tcatcttcga
     1561 aacaatacgc ccaaataact tctcccatcg ctaatttttg ttcatcaaca gagagtctct
     1621 tcaattgttc agctatggtt tcagcagccg aagtggcttg ttgcaagtaa tctttaggat
     1681 caaatattgg aaagttcaaa tgatatcttc gttcttccct cttgattaat cctgcatcga
     1741 tcaataaatc aagaaaatgg gaaaatttat tttgcgaaaa ttcttttttc aattctctta
     1801 gaatcgtttc ttgttctcga tgttcatata gataatcggt caatgaagaa aaaagcggat
     1861 catcggtcag tttttgataa aagttttcct tattttttga ataatagttc atatatgttc
     1921 tcttttctat ccgttttatt ttacttaaaa ggcttttacg taaaaaggag gaagaacaag
     1981 agtcataatt gactgttatt ctcctccatt tcttcggcct ttagtatgtt ctatgtttct
     2041 ttttatatca atcgcttatc cattcatttt gaatgcttca tactaccatt tgattagcta
     2101 aatacagccg tcaattgagg tacgacttgt tttttacgtg aaacgacacc tttaagcaaa
     2161 gcacggttgt tggtcaaagt tgtttggaaa gcttcttcta ctttttcttt tcctttacca
     2221 gcaacaagta attctgaatc actgtctaag atgtttgtga caactaatac gaacagatcg
     2281 tagccatttg ctttcatttc atcttccatt gctgcttcta gctctgcttg tcgatccatc
     2341 acttcattaa ggtccactgt attgacttga gcgatacgaa cgttggaact gcccattggg
     2401 aaactttttg catccaaatc aagaagaacc gcagctgatt tatcacttag atttgttcct
     2461 gctttcaaca tttcaagtcc ataagtgttt agatcgactt cagcgattgc tgcaagttct
     2521 tttgcagctg cttcatcttc aggcgtacat gttggtgatt tgaacaacaa tgtatctgaa
     2581 atgatagctg agagcatgat tcctgcgatt tctttaggaa ttgttacttg gttttcttta
     2641 tatagtttta aaataatcgt acttgtacat ccaactggtt ctgcacgata gtacaatgga
     2701 ttagctgttt caaagtttgc aatacgatgg tggtctacta cagctaaaat atttacttcg
     2761 ctgatatctg aaatactttg ttggaattca ttatgatcca caagcatgac atctgcattt
     2821 tctgctttag ctgactctac tactcgtggt gcagatagct gaaagtggtc taatgcgtat
     2881 tgtgtttctt cattaggtgt acctagagca actgcttctg tttctttacc taattcattt
     2941 tgcaagtgtg aaaacgcaat ggctgccccg attgcatctg tatctggatt ttggtgacca
     3001 aaaactaaaa tttttgacat gtcattcgct cctttgtgat ttctcctatt taataatagc
     3061 aaaaaaacgg aaagaagcaa tacttatctt ctttccgatg cgacctatcg cgttaaatat
     3121 tttgtatact cttctgtatg aagcaatcgt ttagcatttt cgacacgttc ttttgtaggt
     3181 ggttcgatcc cttctaatgg atatttcaaa ccaagtgttt cccacttgta ttttcccatc
     3241 gtatgataag gaagaatctc tactctgtct acatttgaca aggtttgaat aaagttgtct
     3301 agtcggatca aatattcatc ataatcactt cgctcaggaa ctagtacatg gcgaatccaa
     3361 accggtttgt tgatttttga caaatattga gccatttcta aaatattttc attgtcccaa
     3421 tgcgttagtt ttttatgctc ttcattatcg atgtgtttga tgtcaaataa cagtaaatct
     3481 gtatacttca tcaattcttg aaaacgtgaa aagaaaggtt cttcatacgt aaaaggtttt
     3541 ccgcatgtat ccaatgtagt atgcatcttc cgctcttttg cttttttaaa tagttcgatc
     3601 aaaaaatcga tctgcaatag aggttctcca ccactaacag taataccacc tttatctccc
     3661 cagtaaggtc tgaagcgttc tgctttatct aaaagttcat ctgctgtgta ttctttccct
     3721 ccgccaatat tccaagtatc tggattgtga caaaattggc aacgcatgcg gcatccttgc
     3781 ataaacacga caaagcgcag tccaggtccg tctactgaac cgaaagtttc gatagaatga
     3841 acatagccga ttgttttttc ttccatttaa gttcacatct tttctttgat ttcattttaa
     3901 gtacaagtta gctgtcgaag cgaagctttc tgaacaaaat ttttcgcaat tgccgcttcg
     3961 ttatgtgtaa aatttacagg gacggaacat acaagtcctg cccctgtaaa tcgtttcttt
     4021 caaataagca tactgattat tcgattttga tgcgatggct attcaacgtc ctttcgacgc
     4081 taagtcgaaa aacttttttc acgaactttt tacattctgt cgtgtgaagt tctcatcaag
     4141 acatctaatt gttgttctct tgtcaagtca cggaatttaa ctgcataacc agatacccgg
     4201 atcgtcaagt tcggatattt cattggattt tccattgcat cacgcaataa atcattactg
     4261 aatacattga cattcaaatg ataagctcct tttgagaagt atccatctaa gacattacgc
     4321 aagttttcga ttcttgtatc ttgttctttt ccaagacctg aaggattgat tgtttgggta
     4381 tttgaaattc catccaatgc acagccgtaa ttcattttag ctgttgagtt caggctagct
     4441 aataacccac tttgttccgc attgtaactt ggatttgctc ctggcgcaag tggttttcca
     4501 gcttcacggc catcaggagt atttcctgta tttttaccgt aaacaacatt tgaagtaatc
     4561 gtcaataatg aagttgttgg ttttgagttg cggtaagttt tatgacgttt gatttgtccc
     4621 atgaagtatt ccaagatcca tgaaactaat tcatctgcac ggtcatcatc atttccgtat
     4681 ttagggaagt cgccatccac ttcaaaatca actgctattc ctttttcatt acggattgct
     4741 tttacttttg catatttgat cgctgacaag ctgtctgccg catgagagat cccagcaata
     4801 cctgtagcaa atgtgcgttg caagtcagaa tccatcaaag ccagctgagc tgcttcataa
     4861 gaatatttat catgcatata atgaatgatg ttcaacgtat tgacatatag ttctgctaac
     4921 caatccatca tattcatgaa gttatgtttc acttcttcaa agtctagtat ttctgatgtg
     4981 attggacgat attctggtcc aacttgggcg ccagtcactt catcgactcc gccattgatg
     5041 gcatataata gtgttttagc caagttagca cgagcgccaa agaattgcat gtcttttccg
     5101 acaactgttc ctgatacaca gcaagcaatc gcacaatcat cagaacccca ttgttcacga
     5161 agctgattat cattttcgaa ttgaatcgaa gaactttcga tagcgattct gctagcatat
     5221 gttctgaatc cttctggaag gtgttcagaa tacaaaacag tcaagtttgg ttctggtgaa
     5281 ggtcccatat ttgttaatgt atgaagcaga cggaaatcat tctttgtaac taatgaacga
     5341 ccatctaatc ccatacctgc aatggataaa gtagcccaga tagggaatcc tgagaataat
     5401 tggttatatt caggtgtacg cgcgaatttg accatacgta atttcattac taagtggtcg
     5461 atcaattctt gtgcttcttt ttctgtgatt actccattat ccaaatcacg ttggatatag
     5521 atatcaagga atgctgagat acgtccgatt gacatcgcag caccattttg tgatttgatt
     5581 gctgctaaat atccgaagta tagccattgg acagcttctt ttgcatttgc agctggttta
     5641 gaaatatcat agccatattt cgcagccatt tctttcattt gcgctaatgc acgatattgt
     5701 tcagtgattt cttcacgtaa acgaatcact tcttcactca ttgtatgata accagtcata
     5761 tcatgatctt ttttcttttg ttccatcaaa tagtcgattc catacaatgc cacacgacga
     5821 tagtcaccga tgatacgtcc gcgaccgtaa gcgtctggaa gccctgtaat gattttgttt
     5881 ttacgagcag cacgcatttc agcagtataa gcgtcaaata caccttgatt atgtgttttg
     5941 cgccagtcag tgaaaatatg tgtcaattca tcgtttggtg tatagccatt gctttttagc
     6001 gcattgttag ccatattgat tccgccgaac ggcatgaatg cttgtttcaa aggtttgtct
     6061 gtctgtagac cgacgatttt ttccaaatct ttatccaaat agcctgcatc gtgtgaagta
     6121 atagttgata cgatttctgt gtccatatca tagacaccgt tacgctcgaa ttggatatca
     6181 tttaactttt gcaattcatc ccataatttt tctgttgctt ctgttgggcc ttccaaaaag
     6241 ctgtcatcac cgcgatattc agtgtagtta ttttgaataa agtctctagt gtctacgcct
     6301 tctttccatt tggtaccttt aaatccattc cattgctcca ttgtatggac ctcctaaatt
     6361 tctgttttat gtaacagttt ttacgtgatt agtataacac agcaccctat ctttgcaagg
     6421 gttttcttgt tttttattta acaattttta tgttttattt acatatattt aaatcaacgt
     6481 tgacatacca acatttaaga attgtgaaca ctttcatctt tttttgcaaa cgtataagga
     6541 tttattaaaa atgaatgaaa aagagacgcc acctgtatta atacagatgg cgtctcttca
     6601 ttaaaatact acaacagtta cgactcgatg aaaatctcag gagcatcgat aacttcgatg
     6661 acttctccac cttttttctc atccatcgca aatgatccat tcgatgttcg atcacctata
     6721 gggaaatcag ttgggtcaat cgttaactgc tgcccttttt gattcatcaa ggttatttct
     6781 tttgatcttc cttccgacat atagataata cggtgcggat tttttttcag ctcacgcatg
     6841 accgtcactc cccgcttcgc acgtgaagtt tggctgattt cttgagcaag catgcgttta
     6901 acagctcctc gctgagtaac gattacgatc ggcgtatcac cttctgctaa cacaagcagc
     6961 ccgttgataa tcctatctcc atctttcaaa ttcatagact tcactccagc tgcctttgct
     7021 ccaacaacgg gaacatctgt taaaggatag cgcaaaccca ttccttgatg cgttactaag
     7081 aaaacatcta agcctggctg atcttctttc agatacacag caatcagttc atcttcttcc
     7141 gcctttagct tcatacagct gagcggacgg ctcttatagg tgcgccatgg ttcgaactct
     7201 gtcattcgtg tttgcttgat caatccattc ttgctgatga atacaaatgt tttctctgct
     7261 tgaatttttt gatatgggaa aacagctaaa atcgattcgt ctacagacag attggagatt
     7321 gtttgagaaa tgtgctcacc agcatctttc catttcaaat cgggtagttc atggacaggg
     7381 cggtagatca catttgcttt atttgtgatc agaagaacat ggtccaatgt actcaattca
     7441 cctgagtata gcaagtagtc gccttctcgc atcccgattt cttctggttt ggaagcacta
     7501 taagaacgaa ggcttgttcg cttcacatag ccttctcgcg tcacactgac aattacgtct
     7561 tcttgtgcca ctaagacttg cgtatcaatt ttgatttctt cgatttcgtc ttcgattgtc
     7621 gttagacgag cggaagaata attttttttc acttctcgca attctttttt cataacagag
     7681 aaaagctctt tgtcatttga aaggatttta ttcaactctg tgatttgcgc aatcaagctt
     7741 tcggattcct tttgaagctc tgtgatatcc gtgttcgtca aacggtataa ttgcaatgtg
     7801 acaattgctt ctgcttgttc ttctgtaaat tgaaagacgt cgactaaatt ctttttggcg
     7861 tcttttttgt ctttactttc acgaatcgtt gcgattactt catccagaat cgataaagct
     7921 ttcatcaatc cttctacgat atgctgtctt ttttgagctt tggctaaatc gaattgacta
     7981 cgttttgtta tgacttgctt tcgatgctcg atataacttc ttaaaatgtc tttcagtcca
     8041 acttgatgcg gagtcatatg gtcgatagcg accatattga aattgtaatt gatctgtagc
     8101 tcagtgtttt tgaacaaata attcaaaatt ccttgagcat tggcgtcttt tttcaattcg
     8161 acgacaattt gcagacctgt tctatcactt tcatctcgta cttcagctat tccatcgatt
     8221 ttcttattca aacggatctc atccattttt ttcaccaagg tcgctttatt tacttcatat
     8281 gggatttctg tgatcacgat ttgctgttta ttacccttca ttgcctctat ctttgttttc
     8341 gaacgaagaa taacttttcc tcgtcctgtt tcataagctt ttttgatttc atcttttcct
     8401 tgcaagatcc ctcctgttgg aaagtcaggt ccaggaatat attccatcaa tttttcaagg
     8461 ctcgcttgtg gatgatcgat catatagatt gtgccatcga tcacttcagc taagttatga
     8521 gtcgggattt ctgttgcata tccagcagat atacctgtcg atccattgac gagaaggttt
     8581 gggtatcgag ctggaaggac tgttggttct ttttctgtat catcaaaatt ccacacgaga
     8641 tcgactgttt ctttctcgat atctgctagc atctctccac ttaacttgga taatctggct
     8701 tcagtataac gcatcgcagc aggtggatca ccgtccatac tcccgttatt cccgtgcatt
     8761 tcgatcagta cttcccgcaa cttccagtct tggctcatgc ggaccatcgc ttcataaata
     8821 ctgcggtcac catgagggtg ataattcccc atgatatttc cgacagattt tgctgattta
     8881 cggaatcctt tgtcatacgt attgccatct ttgttcatgg caaataatat ccggcgttgc
     8941 actggcttca atccatcacg gatatcaggc agtgcacgtt cttggataat atatttggaa
     9001 taacgtccga agcggtcacc catcacttct tctaaagtta attcttgtac ttcttgtcga
     9061 ttttccatca ggtgacctcc tattctactt caaataactg attgttttca tttttatctg
     9121 ctcgttcttc atctaataaa tcattagaaa ctgaaggaga gatttcagta tcctcttttt
     9181 tatctaagat actgccatct tcttcgagcg taaattgcac gtgattttcg atccattttc
     9241 ttcgaggttc tactttgtca cccatcagtg tggttactcg acgttcagct tgcgcagcat
     9301 catcgatacg gacgcggatc aacgtacggg aagtcggatc catcgttgtt tcccacaatt
     9361 gttccgcatt catctctcca agtcctttat atcgctgcag catatatcct ttgccgactt
     9421 ttttaatcat tgctgccaat tcgtcatctg tccacgcata ttcaatgact tgcttctttc
     9481 cttgtccttt ggatacttta tataaaggcg gtaacgcgat atatactttc ccagcttcaa
     9541 tcagcggttt catataacga taaaagaaag taagcagtaa tacttgaata tgggcgccgt
     9601 ctgtatccgc atcggtcatg atgattactt tgtcgtaatt acagtcttcg atagaaaact
     9661 caggaccgac tcctgcacca atcgtataga tcatcgtatt gatttcttca tttttcaaga
     9721 tatcttgcat tttagctttt tcggtattga tgacttttcc tcgtaaaggt agaattgctt
     9781 ggaactttct gtctcttcct tgtttagcag atccgccagc agaatcccct tcgactaaat
     9841 acaattcatt tttcttaggg tttcgggatt gtgcgggtgt caattttcct gaaagaagag
     9901 attcgccttt tttacgcttc ttcccatttc ggctttcttc tctggcttta cgtgctgctt
     9961 ctctcgcttc tctggctttg attgctttac ggaccaacat ctggctcatt tcgctatttt
    10021 cctgcaaata aaagcccatt tgttcactga tgacgttatc tactacggta cgagcaactg
    10081 gcgtaccaag tttttctttt gtttgccctt caaattgcag aagattttca ggtacacgga
    10141 tagaaagtac agcagctaac ccttctctga aatcactacc ttccagattc ttgtcccgtt
    10201 ctttcagcaa accaactttt ctcgcatatt cattataaga tttcgtcatc gccgctttca
    10261 taccagcttc atgggttccc ccatcttttg tccgaacgtt attgacaaaa gataaaacat
    10321 tttctgaata accgtcattg tattgataag cgacctctac ttcgatacct tctttttctc
    10381 ctgaaaaata aacaactggt gtcaatgtat ctttttcttc attcagataa tcaacaaatt
    10441 ctttgattcc ttcttcgtaa tgaaaaattt ccttgactgg ttcttctcca cgaagatcag
    10501 aaagagagat tttaacgcct tttagaagaa aggcagattc tcttaaccgt tcggctaaaa
    10561 tttcatatga aaatttcgtt gtactgaata ttgtatcatc tggtaaaaaa tggacagtcg
    10621 tcccgtttgc tttttttgtt tttcctatct ttttcaaagt accgtcaggt ttaccgccgt
    10681 ttttaaattt ctgctgatac tctacgccat ctctaacgat cgttacaatt agccatttcg
    10741 ataaggcatt taccacactg gctcctacac cgtgcagtcc accagaagtt ttgtaccctc
    10801 cttgaccaaa ctttccgcca gcatgcagca cagtaaaaat cacttcaact gttggtattc
    10861 cggaagcatg cattcctaca ggcatcccac gtccgctatc tgcaacagtt atactattgt
    10921 cttcatggat ggtcacatca atctcactgc cataacctga taatgcttcg tcaactgcat
    10981 tatcgacgat ttcatagact aaatgatgca agcctcggct gtcagtggat ccaatgtaca
    11041 tccctggtct ttttctaact gcctctaatc cttccaatac ttggatggac gagtcattgt
    11101 attcgttgtt tacttttttt gccaaggcaa acgctccttc aataatactt tgcggcaaaa
    11161 ccgcaccacg attatcatac tagaaaaggt cgtgaaaaaa aagagggtag acgagaaatc
    11221 aattttaatt atctcctaac cgctttttcg acattcccct ctatcgatca tctcttcttt
    11281 gtggtcattt ttatttttgg ttttctttca acttaccaag ttcaatctta atacaacgat
    11341 ccattacgat atctgtacgt cctgcttttc ttagcatctc agctgcttct ttgttttcaa
    11401 tccctaattg tgcccagaac actttagcat cagttttcaa aaaatcacct gctatttcag
    11461 gtaaaaattc actccgtcta aatacatcta cgatatcgat tggatcatcg atttctgtca
    11521 aattcgcata gactttctcg cctagaattt cttcaccagc caataatgga tttactggta
    11581 tgattttata tccattgtct tgcaatacct gtgcaaccat gtaacttgtt cgttctggtt
    11641 tattgctcaa gcctacgacg gcaatatttt ttgcttcttc caaatagttg aaaatcgttt
    11701 cttgtgttgg attttttaca gacatttctg ccaccccttg cttttctttt tattccatct
    11761 attatacttg tatctttcat tttttcaatt gttcctgtta cctattcatg ctaaaataaa
    11821 ggatagtttt caacttgaaa gagggtaaaa aatgaaattg atcttattac ttattttagc
    11881 atatctttta ggttctattc cttccggtgt ctggattggc aaattgtttt tcaaaaaaga
    11941 tatccgacaa cacgggagcg gtaatactgg aacgaccaat acatttcgtg tattaggcaa
    12001 aacagccggg attgttgtcc tgcttatgga tattttaaaa ggtacattgg ctacttgttt
    12061 gccgatgatc ttccaccttt ctgggattaa ccctctttgg tttggggtat gcgccattct
    12121 tggccataca tttccgattt ttgctgggtt caaaggagga aaagctgttg cgacaagtgc
    12181 aggaatgctt ttaggattta atcctgtatt ttttgtttat tcttgtatca tctttatcgt
    12241 tagtttattt tgtacaagta tggtgagttt gagcagtatg atcagtgcag tattgatcac
    12301 tttatctacg atcgtccttc cttatgtagc tcctgtgatt ttggctaagc ccaattggtt
    12361 actgacgatc attgcctttt tagtttcttc ctttatcttt tatcggcata aagacaatat
    12421 caagcgtatc agaaaccata ctgaaagtcg aatcccgttt ggtttgaata gcagcaagaa
    12481 aaaatcctga tactttttca ttccaaagag ataaaacggg agtgtgacaa atcgatgtca
    12541 cactccctta ttacttacag gatagctttt ttacacacct tggtaaacgg tgttccctgc
    12601 taacttctcg gtcacaacct cttacttcac tgtgatggaa tattttgtct tgaagatttc
    12661 atgggcacct agttgattga tccctacttt ttccatcaag tttccactac tgttcaccgt
    12721 gtcagcgatt ccgcaccacg gttcgataca aacgaaaggc gcttcttttg gataaggaga
    12781 ccagatccct acataaggca tatctttata gcttaaagtt acgctatggg aagatttttc
    12841 acttcgaatg gcgtacgcat ttagacccct tgtttcaaaa accaaggcat cttgctcaaa
    12901 aagttcacga ttcaaagcca gattcgtatt tgtctgacct aacgtctttt gatccatatc
    12961 gatgaatggg cctttcaatg gtaggcgtaa tcttgatttc aatggagaaa atgccagata
    13021 gaaatcttca aatgacaatc cttcttcaag aggtacattg aaagcaggat gtccaccgat
    13081 agaaaagaac atttcttttt caccggtatt tgccacttga taatgaaccg tgatgccgtc
    13141 tcctcctaat tcatagctga tcactaattc aaaatcaaat ggatactttt ctaatgtctc
    13201 cggcgtactc cttaagatga aactagccat ttcttgtcca tgttcaatga cttcaaaatc
    13261 catatcccga gcaaatccgt gttgccccat ttgatatgtt ttcccttgat aaacatactg
    13321 atcatctttt aaacgtccaa caaaagggaa taaaaccggt gcatgtctgc cccaatacgc
    13381 tggatcacct tgccagatat actctaaacc atgttctttt gatttcagac tgatcaattc
    13441 cgcaccgtgt tcagcgattt cggcgatcag ctggtcattt tgaataataa cagtcattgc
    13501 cattcctcca acttcgactt actttttaaa atgggaaggc tggaacaaac atttctatct
    13561 gccccagtcc ccttacttct aataactgtg ttttgctaac tcgtctttaa aacgggaatt
    13621 caatactttt aaataagttc ccttcatgcc taacgaacga gattcaatga ttccagctga
    13681 ttccaatttt cgtaaagcat tgacgatcac agaacgagtg atgccgatac tatcggcaat
    13741 attagaagca gtcagacgac cttcatctcc atccaatgct tcaaagatag cttgtacagc
    13801 cttcaattcg ctataagata atgtgttgat tgccatttgg acagccgttg cactccgtac
    13861 atctgcttcg atgcttcgag acttctgata aaggatctgc ataccgacaa ccgtagctcc
    13921 atattctgct aagactagat cgtcatctgc aaacgattgt tccatacgag ataagataat
    13981 cgtacccaaa cgttcgccag ctccaaagat cggaacgaca gtcgtcagac caaatggata
    14041 tttctctctt gactctaccg gaaatgcagt aatatcacta gtaattggga tattagcttc
    14101 agttttcatc aactgtgcca ctgtatcagt ataagattca ggaaattgtt tctcaacaaa
    14161 cattgttttg attcgttcat tgttcacatc gtgacgttca ttataaccta gtaatactcc
    14221 gtcacttcct atgatatagg tgttgctgtc aagaatacca cctagtgtga cagccatttt
    14281 atcgtaaggc aaatctgctt gcatgtcaaa agtattcttt tgctgcaaaa gtgtgtttat
    14341 cttgcgggtt ttcgttagca agtctgtcat tgtcgtcctc cgatttttta taaaatatag
    14401 cgacttaaat cttcatttgc cgcaattgag ccaagtttgt catccacata tgcttctgtg
    14461 atcgttattt ctcccatttg catatctggt gcttcaaata atagatcttc tagtagtttt
    14521 tccaaaatgg tatgcagtct gcgtgcgccg atattatctg tgtcacggtt gacatcgaaa
    14581 gcgatttgag cgatccgttc aattgcttct tttgtaaacg taactgacac attttctgta
    14641 ccgatcaacg cgacgtattg ttttactaaa gcattatttg gttcggtcag aatcttcaca
    14701 aaatcttcag ctgtcaaatc atctaattct acccgtatcg ggaagcgtcc ttgaagttct
    14761 ggaattagat cacttggttt agacaaatgg aaagctcctg aagcaatgaa taggacgtga
    14821 tctgtttgga tgatcccgta ttttgtgttt acttgtgaac cttcgacaat tggcaatatg
    14881 tctctttgga cgccttctct ggatacttct ccagattgct gtgatttaga agtgatctta
    14941 tcgatttcat cgataaaaat aattccactt tcttccgcta atttgattgc ctcactatgg
    15001 atatcagcat ccttgactaa ttttgctgat tcttccttga tcaataactc taaagcttct
    15061 ttgactgtta ccgtccgttc cacttttttc ttcggtgtca aagcacctag agtttcattt
    15121 aggtcgatcc ccatttgctc caatccattg ttcatcattg gtgcaggttt cttttggtct
    15181 tcaacttcaa tggttacttc acgactatcc agtaaaccct tttgtagttg ttccaagact
    15241 gtacggcgat tgattcgaat atcttctgtc acttcttctt gcggttcagc ggtattttgc
    15301 tgactagcat taaaaagatt catcatttgt tcaaattgat tagtggattg tttctgttct
    15361 tttttgatac caggtaccaa gatttttacc aaacgttttt ctgcattttt tttagcactt
    15421 gtgtatacac ggctgtactg ctctttttct acgatttgga tcgcattttc tactaaatcc
    15481 cgaaccattg attctacatc tcttccgaca taaccgactt cagtaaattt tgttgcttcg
    15541 actttgacaa atggtgcatt gacgattttt gccaaacgtc gtgcgatttc tgttttaccg
    15601 acccctgtag gtccgatcat cagcatattt tttggtgtaa tatcttgttg cattttttca
    15661 tctaactgca aacggcgata gcggttacgc aaagcaacgg caacagattt ttttgctgcg
    15721 tgctgtccta cgatatattc atctaattca cttacgattt gtcttggtgt ttgttttaat
    15781 tcatacatat ctactcacct cttacagttc ttctacgata atattatgat tagtaaagat
    15841 acagatatct gcggcaatat ttaacgcatt ttctgcaatt tctttcgcac tcatgtcgtt
    15901 ttgagcatag tttttcattg ctcgggctgc agccaacgca aaattccctc ctgatccgat
    15961 agctaagata ccatcatctg gtgtgataac ttctcctgta cctgaaacaa gaagcatttc
    16021 tttatcattc attacgatca gcatcgcttc aagtttttgc atagattgct gtgtccgcca
    16081 ttcttgagct aattcgacag ctgcgcgttg tagattcccg ttatattcgt ttagtttgcc
    16141 ttcaaatttt tcttctaaag taaacgcatc tgcaacactt ccggcgaaac ctacaacaac
    16201 ttctccattg tagatacgtc gtactttctt tgcagtgcct ttcatgacaa cagattcacc
    16261 cattgttact tggccatctc cagccatagc aaatttcccg tctttttcga cggcacagat
    16321 agtggttgaa tgaaatggtg attcgaccat gtttgtttcc tcctaaattt gttcttttcc
    16381 ttatttttac gctcttggat ggaaagaacg atagtttttt tgtaaacttt cttttgttac
    16441 atgggcatag atctgggtag tcgataaatt ggcatgtcct agcaattctt ggactgtccg
    16501 caaatctgcc ccattattta ataaatgagt agcaaatgta tggcgcagca tgtgaggatg
    16561 gatatcacta tccaaactgc tttttttgat catttgattc aagacatatt caattccagt
    16621 aggcgtgatt ggttttccat gatgattcac aaaaacgata tcatgctctt cgtgatattt
    16681 tgtcatcaat acctggcggc cttccctgaa atattcttgc agagcatctt gagcaaaaga
    16741 acctagagga gcatatctct ctttgttccc tttaccatgg atcaacatga cctctgaatc
    16801 aaaatcaatg tctgataccg acaaactgct acactcactt aaacggatgc ctgacccgta
    16861 aaggacttcc agtaaagcgc ggttcctcaa atccaaagga ctatctcctg ctacgctgtc
    16921 aaaaagtgct tgcatttcat tttcataaaa aaaacgtggc agtctcaaat ttttcttttt
    16981 gagatgaacg taagaaaatg gattttcttt tatcacttct tctttgagaa gatactgata
    17041 aaacgaacgt aggctggcta tcttgcgact gatagaattt ctgctatagc taggacgatg
    17101 atcagctaat gcactcaaat agacacgaac cgtaaaatat tccgccttca gcagatcagc
    17161 gtctccagat tcctccaaaa aatgtttgaa atggagaatg tcttcttcat atgcttcttt
    17221 cgttttttct gaatatcctc tttcgacaat caaataacgc aaaaattttt gttcccacgt
    17281 ggcttctgtc attttctcac cttccttttg tcccagagaa taatttagca cagcgtttat
    17341 aaaaagacaa ataaattgtc agaatttaac caatatttct aagtttgttc acaattggcg
    17401 caattcctca cattttgttt gagaaattct taagaaaaac gcaagaaaaa gagcgagact
    17461 atccgtctca ccctcttcat ggagtttcct tactcatttg ggattgcagt tttttctaat
    17521 tctgttttta ttgcctcatt agcttttaaa gctcgttgtg caattgcttc atatcttgct
    17581 tttttgtctc gaatgcgctc tggcagttca ggaaacagac catagttggc attcatcggt
    17641 tggaaatgtt ttccttccgc atgagtaatg tagtaagcca tacttccaat agctgtttct
    17701 tgtggaaact cgactggttc catttctttt gctaagcgtg cggcgttgat tccagcaagt
    17761 agtccgctcg ctgcactttc aacatagcct tctacaccag tcatctgacc tgcaaagaac
    17821 agatcttcgc gtttcgttga ttggtaagtc ggtttcagca attctggcga attcataaag
    17881 ctgtttcggt gcatcacacc atagcgaacg aattcagcat tttctaaccc aggaatcatc
    17941 cggaaaacac gtttttgctc gccccatttc aagtgagtct gaaagcccac gatgttgtat
    18001 aaagaagctg cagcattgtc ttgacgcagc tggataacag cataaggacg tttgccagtt
    18061 ttaggatctt ccaatccaac tggtttcata ggtccgaaaa gcatcgtttt gaatcctcgc
    18121 tgtgccatta cttcaattgg catacatcct tcaaaatatt tttctttctc aaaagattta
    18181 agttctgcca cttcggcatt gattagtgcc tcatggaaag ctttgaattc ctcttctgtc
    18241 atcggacagt tcaagtaagc tgcttctcct ttgtcatagc gggacttcaa atatacttta
    18301 tccatatcga tcgtagcttt gtctacgatt ggtgcagctg catcatagaa ataaaaacca
    18361 tctgaaccat tgaatgcttt gatgctttct gctaatggat gagaagtcaa aggtcctgtt
    18421 gctactacgg tgattccttc cggtatttca gtaatttctt cgtttatcac tgttactaat
    18481 ggatgctctt tgacttttcg ggtaattgtt tcagaaaaag aatcgcggtc aacagctaag
    18541 gcaccaccag ctggcactgc tgtttcatct gccgaagtga ttactaccga attcaatcgg
    18601 cgcatttctt ctttcaataa ccctactgca ttagccaaac tgtttcctct taatgaattg
    18661 gaacatacta gttcagcaaa attacttgtg tgatgcgcct cagtcgattt gactggacgc
    18721 atttcatata aacggacagg gactcctgct tgtgcgatct gccaagcagc ttcacttccg
    18781 gcaagccctg ctccaacaac atttaccatt ttcaatgaaa gaacctcttt tctattattt
    18841 ttggatgtct tcttcgtaat ctccatttac acagactact tgtttaccgc ctttgacttt
    18901 cttctcgacc agatatccgt cacattttgg acaatttctg cccactggtt tatcccaaga
    18961 agtgaattca cattctggga aacgggaaca accatagaat agtctgtttt tcttagattt
    19021 tcgttcaatg acttgtcctt catgacatac tgggcaagtc acaccgattt cttttacgat
    19081 tggtttcgta ttccgacaat caggaaaatt actgcaagca taaaacttcc catatttccc
    19141 taattttatc accatcggat gtccacagac atcacaatcg aatcctgctg gttcatcttt
    19201 gatttgaatt ttttcgattt tttcttccgc attcgtcagt tctttttcaa acggtttata
    19261 gaaacgatca accactttga cccacgcttc cgtgccttct tcaattttat ccaaatctcc
    19321 ttccatttct gctgtgaaat ggatatcgac gatttgaggg aagaattcac agattaatga
    19381 attgacaatt tctcctagtt cagtcggctc aaatcttttt tgtgtcaatt ttacatagta
    19441 gcgtctttgg atcgtatcaa gtgttggtgc ataagtggaa ggtcgcccta ccccgttttc
    19501 ttctagtgca cgaatcaacg tagcctcact aaaacgtgct ggtggttgag taaaatgttg
    19561 ttttggttca atatcaacag attggactac atcgccttct tcaagttctg gaagaatatt
    19621 ttctttctct tctttgccgt catctctacc ttccacatac acttgcatga atcctttgaa
    19681 tttgattttt gaaccgttcg caatgaagat gacaccattt tgttctaatg tcactttcat
    19741 tgtatctaaa atagctggtg tcatctgact agccacaaaa cgtgaccaga tcaatgtata
    19801 aagtttcaat tgatctttat ctaaatattg tttgagttca tctggtgtac gaagcacact
    19861 cgttggacgc acagcttcat gggcatcttg tgcgccttga gcatttttga tctttcggtg
    19921 atcatgtcta gaaaattctt ttccatacgt atcttcaata aatgctgcgg cctctgcttt
    19981 tgctgtatcg gcaatccggg tagaatcagt acgcatgtat gtaatcaagc cgactgttcc
    20041 ttgtttaccc aaagtgattc cttcatataa ttgctgtgct accatcatgg tttttcttgt
    20101 tcgaaagttt aacttacggg cagcttcttg ttgcaggcta cttgtagtga aaggattcgc
    20161 agggtttctt ttgcgctctt tcttttctac ttttttaact tcgaagtctg ttccatcaat
    20221 tctagcagtg acttctttta cactttgtgc atctggtaat ttctttttct taccgtcgat
    20281 tccccagaaa tttgctttga atttcttgcg agctttttta aaattaccgt cgatgctcca
    20341 atattcttct ggaacaaatt ctcggatctc tttttcacgg tcaataataa tttttaaagc
    20401 gacagattgg acacgtcctg cacttaaccc tttcttgacc ttacgccaga gaataggact
    20461 gatcgaataa ccaacgattc tgtctaaggt acgacgagct tgctgtgcat ctactaaatc
    20521 tacatcgatc gttcttggtt ccttaaaagc tgctttgact gcgtcttttg tgatttcatt
    20581 gaagaccacc cgattttttt ctttcagatc caacccaagt aggtacgcta aatgccaagc
    20641 aatcgcttct ccttctctat ccgggtcact tgccaagtaa actttttgag ctttttttgc
    20701 tgcggctttc aggcttttga tgacatcgcc ttttccgcgt atagaaatat aatggggttc
    20761 ataattgttt tcaaaatcga tacccatttt acttttcggt aaatctctta tatggccaac
    20821 actggccatt actttatagt ttcgtccaag atatttctca attgtcttgg cttttgcagg
    20881 tgattctaca atcactaaat atttatacgc cattactgaa tggactcctt tccattgtcg
    20941 tgttcctact attaaaagag gattcataca aatcgtagct aatcatatcc attcagaaaa
    21001 cgtttgtcaa gaaaagcaaa tgaaaagtat ggtgttattc tttaaattct gccaagatat
    21061 cttcacccga aaccgtgcaa actgcaccgt cttggatcaa atgatgacat ccggtagaat
    21121 gttctgttaa aatatttcct ggaacagaaa aaactgttct tccaagatcc aatgcttgct
    21181 gtgctgttat cagagagccg ctttttttct tggcttcaaa gacacatgtt cctttagaca
    21241 aagctgcgat aatccgattt ctcattggaa aacaatgctt tttcgggccg gattgaggag
    21301 gatattcact caaaagaaga tgatcatttg caatcacctc ttgcaagtca cgattttcag
    21361 caggatagac ttgctctatc cctgttccaa tgactgcgat tgtccgtcca ccatgagaga
    21421 ttgctgcttg gtgaccgtat gtatctatcc ctttcgcgca gccactgacg atcacatatc
    21481 cagcttctat cactggtttt agtaaataat aaactgtttt tttggctaaa gcagaagtgt
    21541 gtcttccacc aacaacgctt aacatgttac aagaaagtaa agatagatca ccattataaa
    21601 acagtacaat tggcggatag gtcaagtgaa gcagctgagg gggatatttg ggatcaaagc
    21661 aagtgataaa cgactgtttc gattggattt tgccccagtt agcgtgaatt tcctgccaac
    21721 ttttgcgaaa cagctcccgg tgtttggtta ttttacctat cttaatgatg tcttcaaatc
    21781 cgatttcttt ataaggatac tgcatcatcc agttagcaag tgcccatttt ccttgactac
    21841 tgattccttt tgtataagca agtttcaata aattttcttc tatttgatac atcataaaac
    21901 cgcctttcgt ctctctttct aataagatac gtaaaagcgg ctgtttttta ttgtaaatat
    21961 ttttttacag gtgcaaaact tttacgatgg attggcgtaa ttccttgtcg ttcaagtcct
    22021 tctaagtgtt cctttgttcc ataacctgca ttatgcgaga aaccatatcc aggataaagc
    22081 acgtcatatt ctttcatcaa ttggtctcga tatactttag caatgatact tgcagcagca
    22141 atcgacacag atcgtgcatc ccctttgatt attttttcct gctgaatagg caaatccaag
    22201 gtcatcgcat cgatcaacaa atagcttggt acaatggaca atttttctac tgcttggatc
    22261 atcgctaatt tacttgcttc ataaatattc acatcatcga ttgtctgctg atcaacgatc
    22321 ccgatcccga ttgccagggc tttttcttga acttgttgat aaagaagttc tcgctttttc
    22381 tctgataatt gtttggagtc attcagccct aatatgatct catttttcgg caagataact
    22441 gcagctgcca cgacaggtcc agctaatggt cctcttccta cttcatcaat gccggcaatc
    22501 cattggtgac cttgtgcgta cccttgcttt tcaaagcaaa acatttcttc atatttttcc
    22561 ttaattaatg cttccttttt caatcgtcgt tcaaaagaag ccagtgcttg ctgcacgcct
    22621 ttgcgtggat catttcttaa tgattctagt gtaggatcat ctattctctg tatgcttttc
    22681 aatcgttctt tgatactttg gatggattca gtcatcactt ttttgctcca tttcttcgca
    22741 acgatccagt gtgtatcgtc caagttttcc ttgacgaata tcatgcacga ctaattcgct
    22801 gccacgatca tagtcttctt tatacccacg ttttgctgta atatccatca gtaattccgg
    22861 tgccaatttt tcttcttgct cgattgataa accataacga tcgctgattc ttcctgaata
    22921 atatcgggca aaaaagtcta atccgtaaat cgctaaatca tctaaatgga caagctggtc
    22981 tttgatcgcg cctgtcagcg ctagtttttt gccgatttct tcatcttcaa atttgggcca
    23041 taaaatccct ggtgtgtcta acaattccaa ctcattacct aaacgaagcc actgctgacc
    23101 ttttgttacg ccaggcttat ttcctgtctg tgcaattttc ttgcctacta gtcgattcat
    23161 caacgtagat ttcccaacat ttggaatacc taagaccatt gcccgaatag cgcgaggctt
    23221 gatcccccga gcagcatctc ttgcccgttt ttctttcaat gcttctttag ccgcaggtac
    23281 tagaccttta attcctttac tttcttgtgc cgtgatagct aatgcaagat ggccttgttt
    23341 acgaaaatat tcttgccaca gctttgtctg ttgtggatct gccagatccg ctttattcaa
    23401 taaaacaagg cgaggttttt gctgaatgat ctgatctagc attggattac gagaagataa
    23461 aggtaatcgt gcgtctacca attcaaatac gatgtccaca tatttcattt tttcagaaac
    23521 ttctcgtcgg gcttttgcca tatgtcccgg aaaccattgg atggtcattg ttctctccat
    23581 tcctttcata ccggaatatt ccccggcttt acttgttttt ttctttttca atataaattc
    23641 acttttttat tataaagaaa ttccatctat tagttaagtg aaagtgaacc agtcctcatt
    23701 ctatcacatc ttcacttttt tgatgatgca aatttttatt ttggtttcta tatatgaatc
    23761 taaatttttg acagcgtttc ctaaataaga gatgatagac gaacaaaaca gaaaggagac
    23821 gaaacaaatg aatagtatgg tttttgttaa ttttcctgtt gctgatgtca atcgttccgt
    23881 tgctttttat gaaaagctgg gttttaaaaa aaatgaagaa ttttctactg aagaagctgg
    23941 ttcaatggta tgggatgaca ctttttggat aatgcttctc tctcatgatt tttataaaaa
    24001 atttataaaa gataaagaaa tagctgataa taaaaaaaca agtggtgctt tgatttcgtt
    24061 tagtgttgaa aatgcggatg aagtaaaaaa atttgctcaa attgcaaaag aaaatggtgg
    24121 tactttccac gaagtagata tgggaatgcc tgaagaagaa atgttttcat tagaagtcca
    24181 agatctagac ggaaatacat tagaacccgt atggatgaag atgtaagcag ataactaatc
    24241 tgacgttttt tataacaaaa aaacaaaggc ggatgatacg ataagtattc tccgcctcta
    24301 ttttttttat tcgtacaatc tcttttttac agtttataaa agcagtcttc tcaccctttt
    24361 tttaattgtt agatgccagt gtatgtaaat caccaatgtg tcatgacaca ttggtgattt
    24421 tatggtatcc ttttattatt aactgacaaa ggagagaata ttttgaacac tttgattgca
    24481 aaagacgttt ttagattgcc cgaaatgaat gcaagattgt tgtcgacctc tgctctttta
    24541 ttagcccttg atgtcatctt atacaagata gcgattggtc ctgcttattt tcaaatcaca
    24601 ttcggatttt taagtatggc gcttatgggt tatctatatg gaccgatctg ggctggtttg
    24661 cttgaaatgt tgtcgaacac tttaaatttc acgatatttg gttcaggaac gtttcaagct
    24721 gcttttctta tcacggcttt ccttggtgga tttattaatg gcttattttt atatcataaa
    24781 aagataaacc tgccttccgt catcgctgcc caattattga ttatgatccc agtcagcctt
    24841 tttatcaatt cttggctgat gtctgttttg tttggtgcag atttcaaagt aatttttcta
    24901 ggtagattca tcaggaatat gatccaaatc cctattcaaa taatggttgt ttattcattt
    24961 atcaaaactt tagaggctag aaaagtgttt aaacgagctg tttgatttta cgtactaagg
    25021 tagtaaactg attttggatt aaacaataaa gcgtgcagtc tcaacagact gcacgcttta
    25081 ttgtttctca ctggatccat tttatttcat caagtggata atagacaaat cttgctttac
    25141 ctagaatatc agttttattg atcgtgccaa acgaacgact atcttttgaa actcttcgat
    25201 tatctccaag tacaaaataa ttatcttccg gaagcttgga ttgtcccgtt agctcttgta
    25261 gtgtaaagtc cgtcgtatag gatgcatttg catgatctga tttcaggttt ttagttagat
    25321 aaggttcctt aactactttc ccattgatct tcaattgatc attttggtag ctgacatttt
    25381 ctcctggaag accgattact cgtttgatat aaatcgtgcc atctgctaat tgaaaaacaa
    25441 caatatcaaa tcgtctgatc tccgaaaatt tttccatcac aaccatatca ccttgcttca
    25501 acacgttttc cattgaattg ccttccactg gtaccgggat gaataaaaat ccccgcaaca
    25561 tgaacgcaag gaatacagaa acaagtaagt atttgaatac aagccagaag cgattaatat
    25621 agcgttgttt ttttgtcaat ctctatcaat ccatccctct ttttttcatt ctttctttat
    25681 tataagaaa
//
LOCUS       DAJSKS010000025        25088 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJSKS010000025 DAJSKS010000000
VERSION     DAJSKS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.025"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            12..84
                     /locus_tag="ON396_000899"
     tRNA            12..84
                     /locus_tag="ON396_000899"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ON396_000900"
     tRNA            88..160
                     /locus_tag="ON396_000900"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ON396_000901"
     tRNA            168..249
                     /locus_tag="ON396_000901"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ON396_000902"
     tRNA            267..339
                     /locus_tag="ON396_000902"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ON396_000903"
     tRNA            351..422
                     /locus_tag="ON396_000903"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ON396_000904"
     tRNA            439..524
                     /locus_tag="ON396_000904"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ON396_000905"
     tRNA            535..608
                     /locus_tag="ON396_000905"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ON396_000906"
     tRNA            618..691
                     /locus_tag="ON396_000906"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ON396_000907"
     tRNA            711..783
                     /locus_tag="ON396_000907"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ON396_000908"
     tRNA            811..884
                     /locus_tag="ON396_000908"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ON396_000909"
     tRNA            898..971
                     /locus_tag="ON396_000909"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ON396_000910"
     tRNA            995..1084
                     /locus_tag="ON396_000910"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ON396_000911"
     tRNA            1097..1170
                     /locus_tag="ON396_000911"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ON396_000912"
     tRNA            1189..1264
                     /locus_tag="ON396_000912"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ON396_000913"
     tRNA            1266..1336
                     /locus_tag="ON396_000913"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ON396_000914"
     tRNA            1350..1423
                     /locus_tag="ON396_000914"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ON396_000915"
     tRNA            1448..1535
                     /locus_tag="ON396_000915"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ON396_000916"
     tRNA            1557..1628
                     /locus_tag="ON396_000916"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ON396_000917"
     tRNA            1651..1740
                     /locus_tag="ON396_000917"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ON396_000918"
     tRNA            1753..1826
                     /locus_tag="ON396_000918"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ON396_000919"
     tRNA            1845..1917
                     /locus_tag="ON396_000919"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ON396_000920"
     tRNA            1921..1993
                     /locus_tag="ON396_000920"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ON396_000921"
     tRNA            1998..2068
                     /locus_tag="ON396_000921"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ON396_000922"
     tRNA            2081..2154
                     /locus_tag="ON396_000922"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ON396_000923"
     tRNA            2166..2237
                     /locus_tag="ON396_000923"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="ON396_000924"
     CDS             2503..3696
                     /locus_tag="ON396_000924"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR3113026.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ON396_000925"
     CDS             3826..5298
                     /locus_tag="ON396_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3113027.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ON396_000926"
     CDS             5381..6169
                     /locus_tag="ON396_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR3113028.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ON396_000927"
     CDS             complement(6236..6904)
                     /locus_tag="ON396_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HCR3113029.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ON396_000928"
     CDS             7019..8731
                     /locus_tag="ON396_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3113030.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="ON396_000929"
     CDS             8734..10503
                     /locus_tag="ON396_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3113031.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="ON396_000930"
     CDS             complement(10708..11274)
                     /locus_tag="ON396_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3113032.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="ON396_000931"
     CDS             complement(11274..12230)
                     /locus_tag="ON396_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR3113033.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="ON396_000932"
     CDS             complement(12230..12886)
                     /locus_tag="ON396_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR3113034.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            13256..15274
                     /locus_tag="ON396_000933"
     CDS             13256..15274
                     /locus_tag="ON396_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3113035.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    15344..15550
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15701..18115
                     /locus_tag="ON396_000934"
     CDS             15701..18115
                     /locus_tag="ON396_000934"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR3113036.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            18463..20016
                     /locus_tag="ON396_000935"
     CDS             18463..20016
                     /locus_tag="ON396_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCR3113037.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(20397..22181)
                     /locus_tag="ON396_000936"
     CDS             complement(20397..22181)
                     /locus_tag="ON396_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3113038.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(22196..23917)
                     /locus_tag="ON396_000937"
     CDS             complement(22196..23917)
                     /locus_tag="ON396_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3113039.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            24017..24778
                     /locus_tag="ON396_000938"
     CDS             24017..24778
                     /locus_tag="ON396_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3113040.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7746 a         4617 c         5352 g         7373 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
    13141 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
    13201 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
    13261 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
    13321 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
    13381 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
    13441 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
    13501 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
    13561 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
    13621 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
    13681 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
    13741 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
    13801 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
    13861 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
    13921 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
    13981 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
    14041 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
    14101 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
    14161 ggaaaatggc tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg
    14221 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
    14281 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
    14341 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
    14401 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
    14461 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
    14521 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
    14581 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
    14641 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
    14701 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
    14761 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
    14821 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
    14881 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
    14941 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
    15001 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
    15061 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
    15121 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
    15181 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
    15241 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
    15301 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
    15361 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
    15421 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
    15481 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
    15541 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
    15601 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
    15661 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
    15721 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
    15781 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
    15841 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
    15901 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
    15961 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
    16021 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
    16081 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
    16141 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
    16201 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
    16261 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
    16321 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
    16381 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
    16441 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
    16501 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
    16561 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
    16621 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
    16681 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
    16741 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
    16801 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
    16861 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
    16921 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
    16981 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
    17041 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
    17101 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
    17161 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
    17221 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
    17281 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
    17341 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
    17401 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
    17461 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
    17521 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
    17581 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
    17641 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
    17701 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
    17761 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
    17821 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
    17881 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
    17941 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
    18001 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
    18061 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
    18121 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
    18181 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
    18241 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
    18301 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
    18361 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
    18421 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
    18481 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
    18541 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
    18601 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
    18661 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
    18721 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
    18781 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
    18841 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
    18901 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
    18961 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
    19021 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
    19081 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
    19141 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
    19201 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
    19261 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
    19321 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
    19381 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
    19441 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
    19501 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
    19561 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
    19621 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
    19681 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
    19741 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
    19801 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
    19861 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
    19921 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
    19981 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    20041 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    20101 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    20161 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    20221 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    20281 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    20341 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    20401 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    20461 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    20521 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    20581 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    20641 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    20701 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    20761 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    20821 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    20881 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    20941 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    21001 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    21061 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    21121 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    21181 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    21241 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    21301 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    21361 aaggcgagtt gattaatgaa acgaatggcc ggatagatag caaagttcat aaattgagca
    21421 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    21481 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    21541 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    21601 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    21661 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    21721 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    21781 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    21841 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    21901 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    21961 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    22021 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    22081 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    22141 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    22201 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    22261 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    22321 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    22381 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    22441 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    22501 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    22561 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    22621 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    22681 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    22741 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    22801 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    22861 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    22921 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    22981 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    23041 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    23101 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    23161 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    23221 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    23281 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    23341 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    23401 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    23461 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    23521 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    23581 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    23641 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    23701 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    23761 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    23821 gtacacctaa tgtaccggcg atttgtaaac agatcatcac aaaggtaagg agagcgagtc
    23881 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    23941 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    24001 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    24061 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    24121 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    24181 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    24241 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    24301 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    24361 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    24421 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    24481 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    24541 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    24601 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    24661 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    24721 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    24781 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    24841 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    24901 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    24961 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    25021 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    25081 gagaatga
//
LOCUS       DAJSKS010000026        24936 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJSKS010000026 DAJSKS010000000
VERSION     DAJSKS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.027"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            133..702
                     /locus_tag="ON396_000939"
     CDS             133..702
                     /locus_tag="ON396_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3113041.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(880..1320)
                     /locus_tag="ON396_000940"
     CDS             complement(880..1320)
                     /locus_tag="ON396_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR3113042.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            1478..2242
                     /gene="map"
                     /locus_tag="ON396_000941"
     CDS             1478..2242
                     /gene="map"
                     /locus_tag="ON396_000941"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR3113043.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            2274..3197
                     /locus_tag="ON396_000942"
     CDS             2274..3197
                     /locus_tag="ON396_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCR3113044.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            3273..4412
                     /locus_tag="ON396_000943"
     CDS             3273..4412
                     /locus_tag="ON396_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCR3113045.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            4405..5205
                     /locus_tag="ON396_000944"
     CDS             4405..5205
                     /locus_tag="ON396_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3113046.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            5205..6032
                     /locus_tag="ON396_000945"
     CDS             5205..6032
                     /locus_tag="ON396_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR3113047.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            6010..6744
                     /locus_tag="ON396_000946"
     CDS             6010..6744
                     /locus_tag="ON396_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3113048.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            6844..7710
                     /gene="rfbA"
                     /locus_tag="ON396_000947"
     CDS             6844..7710
                     /gene="rfbA"
                     /locus_tag="ON396_000947"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR3113049.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            7724..8296
                     /gene="rfbC"
                     /locus_tag="ON396_000948"
     CDS             7724..8296
                     /gene="rfbC"
                     /locus_tag="ON396_000948"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR3113050.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            8318..9346
                     /gene="rfbB"
                     /locus_tag="ON396_000949"
     CDS             8318..9346
                     /gene="rfbB"
                     /locus_tag="ON396_000949"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR3113051.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            9444..10295
                     /gene="rfbD"
                     /locus_tag="ON396_000950"
     CDS             9444..10295
                     /gene="rfbD"
                     /locus_tag="ON396_000950"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR3113052.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            10329..12362
                     /locus_tag="ON396_000951"
     CDS             10329..12362
                     /locus_tag="ON396_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113053.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            12406..13686
                     /locus_tag="ON396_000952"
     CDS             12406..13686
                     /locus_tag="ON396_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HCR3113054.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            13896..14702
                     /locus_tag="ON396_000953"
     CDS             13896..14702
                     /locus_tag="ON396_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3113055.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            14714..15934
                     /locus_tag="ON396_000954"
     CDS             14714..15934
                     /locus_tag="ON396_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3113056.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            15924..17510
                     /locus_tag="ON396_000955"
     CDS             15924..17510
                     /locus_tag="ON396_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3113057.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            17549..19687
                     /locus_tag="ON396_000956"
     CDS             17549..19687
                     /locus_tag="ON396_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3113058.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(20055..21038)
                     /locus_tag="ON396_000957"
     CDS             complement(20055..21038)
                     /locus_tag="ON396_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR3113059.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            21416..22807
                     /locus_tag="ON396_000958"
     CDS             21416..22807
                     /locus_tag="ON396_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR3113060.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            22823..23863
                     /locus_tag="ON396_000959"
     CDS             22823..23863
                     /locus_tag="ON396_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR3113061.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            23882..>24936
                     /locus_tag="ON396_000960"
     CDS             23882..>24936
                     /locus_tag="ON396_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCR3113062.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTE"
BASE COUNT         8046 a         4108 c         5097 g         7685 t
ORIGIN      
        1 atatatgtta ctacttgcta tttcagcttg tgaagaaaat caaaaactaa ttttgcatat
       61 caaaaaaaac atgttacaat gaaattaatc aatggtggaa gcgttataaa aaaattaagg
      121 agattaaaaa atatggcaag aaaaaagacg attactagag aacagatttt ggcagctgct
      181 tatgaagtag ctgccaccga tggatttaca cgatttactg cgagaaacat cgcgaacaaa
      241 atgaattgtt ccacgcaacc gatttatctt gagtttaaaa atatggatga cttgagacaa
      301 gctctttttg agcagatcta tgatcattta caatatgaag tgttcactgt tgagcataca
      361 gggaatacga ttatcgatct tgcttttaat tatatccatt ttgcaagaag agagaagaag
      421 ttgtatcgtt cactgtactt agaagaatat ggtggaggaa aagagatgca gaatttctct
      481 tatacttact ttgtgaatac agtgaaacgc gatccagctt atgtgaatct ttcagaagag
      541 cagatcgaat ctttgtataa tgggacatgg atcatagcta ctgggatcgc agctttgatg
      601 tcttcaagca tcatccatcc aactgatgaa cagattgaaa agatgatcca agacagtata
      661 gatgctatct tgaaattcaa agaaccaatc gaaatcgatt gaatatactc aaaagttcca
      721 caaatcagta ggtaacaaga gatgtgtcaa aataaggaaa agttcgtttt tcttacagcg
      781 tatgcatttt taactcccga agaactcttc catgaatgca atgaataaaa gagccataca
      841 gattgaataa atctggatgg ctcttttatt gcaagtatat taaatttttg cgactagttc
      901 tttagcgaaa gcttctaaat tttcgatatc ttcttcttca gcattcagat ccactttaac
      961 attttcggct ccttttgatg cgcctgtttt tgcaaaagca gcatcgaaat catccaccga
     1021 tttgcagaat tcatcgtaaa aagtatcacc agatccgcag acaccgtaaa tttttcccaa
     1081 tagatccaat tcttgcaagt cttcataaaa gtctacgatc tcatcaggca gatcgccatc
     1141 atcgtatgta taagtagcta ctacgcaaat atctgcctcc tcgaattcat ctgcatctac
     1201 ttgggtacat tcgttgattt cgacttcgat atccagattt tcaaatgctt ctgcaacaat
     1261 atcggcgatt tcttcagtgt ttcctgtcat gctagcatat acgatttttg ctaaagccat
     1321 gatgtgcctc ctcgtttata attatttaac aattatgaat tataccaaaa catcattgaa
     1381 aagaaaataa aacctagaga cacctgggga atgtcatttg gctaaatgta tgccgctatg
     1441 ttaaaatgag agggatttgt aaaaaggaga cgaaaatatg attactttaa aatcagcacg
     1501 tgaaattgaa atgatggcag aatctggagc tttattagca gatgtacata aaaatctgcg
     1561 tgattttata aaaccaggaa tcacaagttg ggatattgag gtatttgtca gaaactatat
     1621 cgagagtcat ggcggaattg ctgcgcaaat tggctttgaa gggtatgaat atgcgacttg
     1681 tatcagtata aatgatgaaa tttgtcatgg attccctcgt aaaaagcctt tgaaaaacgg
     1741 tgatttggta aaagtcgata tgtgtatcga tttgaaaggc ggcatttctg attcttgttg
     1801 ggcctatgta gttggagaat ctacacctga gatcgaccga ttgatggaag taacgaagaa
     1861 agctttgtac atcggtattg agcaagcaca agtagggaat cggattggtg acatcggtca
     1921 tgctatccaa acttatgtag aaggcgaaaa tctagcgatc gttcgtgatt ttattggtca
     1981 tggcgttggt cctacgattc atgaagaacc agcagttcct cattacggag aagctggaaa
     2041 agggttgcgg ttgaaagaag gcatggttat cacgatcgaa cctatggtta ataccggaac
     2101 ttggaaaatg aaaatggatc ctaacggctg gactgcgtat acaagagacg gcggactttc
     2161 ttgccaatat gaacatacat tggctattac taaagatggt ccaagaattt tgacatcaca
     2221 aggagaagaa ggcacttatt gatctatcca gtcaataggt acgggagagg tacatgaaag
     2281 tttggaacaa aataaaacaa aatgaaaatc tgatgcgctt tattgagacg actcaaaagc
     2341 gcatcactga ttcagaaatg agtacgacct ctgtcgtcgt tgcttattat ttactcttgt
     2401 ctttatttcc attgattatt gcttttggta atattttacc gtttctccac atcgatcaag
     2461 agactgtact gacctattta cgagaagtga ttcctgagac cgtttaccag tttatcggtc
     2521 ctgcaatcaa agaccttttg acacaaagtt caggaggctt gttatccgtt tctgcattag
     2581 cggctttatg gtctgcaagt caaagtatca atgctttgca aattgcgatg aataaagcgt
     2641 atggcgtaga aaatagaaaa aattttttga tcgtccgttt cttctcacta attgtcattg
     2701 tcttgttcat gattgctatt agtggggtaa ctatcgttct tgggttggga cagctgattt
     2761 tggatgctat tcagccaatc gtacaaatac ccgtgaactt tatcgatcag ttccaaactt
     2821 tgaagtggcc aatcacattg gtagctttat ttgtcatcat gttcttgatt tatttaattg
     2881 tccctaatgc tcagttgaag ttgaagacga tcattccggg cacgatcttc gctactgtcg
     2941 gctggatgct gttatcacaa gtgtttagta tctatgctag gtattttgca acgaggatca
     3001 gcggttatca gatcatagga agttttattg tactcatgct ttggcttaat tttgctgcta
     3061 cgatcatcat tttgggcgga atcatcaatg cagttgttca agaatacgtg acgggtcatc
     3121 aaatcaaaaa acgtcgagat ccaagccgaa aatggttcaa taaattgaaa actaaatttt
     3181 ctcataaaaa aaagtagaaa caagagttct tattgtaaat ttatatcagt aatttataat
     3241 aattatgact cttaattaaa gtaggtgccc atatgcttgt ttcttttagt atggttataa
     3301 gattttttct gacaatttta ttatcactga tattaacacc tgtattcaaa atcatttcag
     3361 tacaaacagg tatggtcgac aaaccaaatg aaagaagaat aaataaagtt ccaatgcctt
     3421 cagcgggcgg actgcctatc tttatttcat ttgttatatc gactttattc ttattccgta
     3481 aaattatccc tcaatcgtat attgtaccga tcttgattgc ttctttagta atcatagtca
     3541 caggagtgct cgatgataaa tatgagctga cacctaaaca aaaaagtata gggatcttga
     3601 ttgcttctct tattatttat tttgtagcag atatacgtat tgattccttc acgcttcctt
     3661 ttattggata tatccaacta ggctggctta gttttcccgt aacgattttt tggatcttcg
     3721 gtatcacgaa tgcggtcaat ctgattgacg gtttggatgg gctggcggca gggatttcat
     3781 tgatcggttt aattacgata ggaatcattg gatatttttt cctccatgct tctacagtgt
     3841 atattccagt tgttattttc tgtcttgtag caagtattat cggttttttt ccttacaatt
     3901 tttatccggc aaagatttat ttaggcgata caggagctct attcctaggg tttatgatgg
     3961 ccgtcctttc actacaaggg ctgaagaacg tgacttttgt ttcatcgatt tctttgctga
     4021 tcgtcatggg agtgccggta acagacacgt tttttgctat cattaggaga aaagcaaacc
     4081 gtgtttcttt ttctacagca gataaaaaac atcttcatca ccgactaatc agtttaggct
     4141 ttactcataa aggtgctgtc ctgacgattt atgcgatggc tttgatgttt tctttcacag
     4201 ctatggtcat gaattatact ggaagaatcg gaacgatcat attgattatt gccatgttgt
     4261 ttgctgcaat tttattattt gaattgattg gtctgatcaa tgagaaacat cagtttattt
     4321 taaaaacatt gcgattttta gggaataaag agtaccgtac gcaagtaaaa cagaaatatt
     4381 tgaagaaatg gaaccgtcat atagatgaat aacaaattaa accagccgat ccttattagt
     4441 attgtgacgg caaacagtga taaaattttt cggacactag ataattttat ttcaactgta
     4501 aaagatgaaa cagcagtaaa aatccgaatt tttgacaaca attcacttcc cgagtacaaa
     4561 aaaagactaa cagagtatag gaaatatccc tacatcgata tctattttca tgatgaaaat
     4621 tccggatttg gttatggaca taatcacaat ctgatagcta gcgattctcg ctatggcatt
     4681 atttgtaacc cagatatttt agtggatgaa gagacagtta cagcattggt cttgttattg
     4741 aaagaacatc cggaatgtgc gatgttagca cctaagattc ttaatgaaga tggaacaact
     4801 cagcacctca tccgtgagca gctggctgtt tttgattacg ttttgcgctt tgtgcctttc
     4861 aaatgggtca aaaaaaagtt tgacaaacga ctggctgtgt ttgaatgccg aggattgcct
     4921 actgatgcta atagttatgt acggatgatc tcaggtagtt ttatggtagc tgatattgat
     4981 aaatttaaag agataaaggg gtttgatgag cgtttcttca tgtactttga agacaacgac
     5041 ttgtgtttga gttttgaaaa agcaggttac aagttattgt acacaccctt ttatgcagtt
     5101 atccattttt acgggaaggg tgcgcatcga aatgcaaaac tgtttcgcgt gtttttgact
     5161 tctatgatga aatttttcaa taagtggggg tggcgttttt tctgatgaaa ccagaaatat
     5221 tagtagtcgt tgtgctttat cagcaactat tcagtcaatc tccagcttat ggaccattaa
     5281 gcaaagcact tagcgaaaag caaattcagc ttattattta cgataatagt cctcaaaggc
     5341 agcaaaatga gctgtttgaa aaagaaaata tcgtttatca tcatgatccg caaaatcctg
     5401 gtttagcggt ggcttataat tttgcgttgt ctcaagcaga tgaaggaaca cgttgtctcg
     5461 tcactctgga tcaggatacg aggctagtcg acgattattt tgaactattg cgtaaagtaa
     5521 cctttacaga tgaatgtgtt gctgcagttc cgatgatttt ttctgggagc cgacaagttt
     5581 ctcccctata ttcagatcgt tatatcaatc gtacagctca agcagtagaa gttgaaggcc
     5641 caacagctga acgaatcatg gcaatcaatt caggtgttgc ctggtctgtc aaatttctaa
     5701 aaaagattgg cgggtttaat cctgcttttt ctttagattt tttggatcat tggctgtttt
     5761 ggaaagtgga tcagttggaa aaaactgtgg agatccttcc tgtacgaatg gaacatgatc
     5821 tttcggtttt agactatgaa aaagtttcaa gtaatcgata ccgttctatt ttacaagcag
     5881 aaaatcgatt ttatcatgaa tatgatcaaa ggcatttatc cagtcaccga cgacaattat
     5941 tgttaagaac agctaaacag ttcttgaccg tcaaaaaccg gcatatctgg cggatgactt
     6001 tgcgctcata tgttaagaat tggaaggtgt gaattcgtgc gttcggtttg tatagctacc
     6061 tttaatggag gcagatattt aagagagcag ttagatagta ttttatctca gctttcagca
     6121 gaagatgaag tagtcatttc tgatgatggg tcagtagatg atacatggga tatattaact
     6181 gaatacgcag aaaaagataa gcgaatccag cttttttcgg ggcctcgtca aggattgatc
     6241 gctaattttg gttatgcgat ccagcagacg aaaggagatc taatcttttt ggcagaccaa
     6301 gatgatgttt ggttggagac aaaagtgaaa aagatcactg actattttga gcagaatccg
     6361 gatcagctag tagtagtttc tgatttaatc attgtggaca gcgagctgca agagatccat
     6421 gattcgtatt tttcgtttcg aaattccaaa gaaggatggc ttaataatgt gataagaagt
     6481 cactatattg gtgctggtat ggctttccgg tcttctttaa aacccgatat tttgccgatc
     6541 ccgtcagaag tgccgatgca tgatatgtgg attggtttgc tgggaggaaa gaagactggg
     6601 ttcctaagag aaccgttaac tctgtaccga agacatgatt taaacgcgag tgagattgaa
     6661 acacattcta aattacggca aaaaatttct tggcgtgttc atctgattac ggcgttgatc
     6721 aaacgaaaat ggtttaaacg ttgataaaag atgtttaaat ccttaatatt tccatgacaa
     6781 atataacaat tgatgagata ataacaaagt gaaatttgaa atcaaaaagg agagcagata
     6841 tccatgaagg gaatcattct tgctgggggc agcggaacac gtctgtaccc tttaacaaag
     6901 gcaacatcga aacaattgat gccgatttat gacaaaccaa tgatttatta tccaatgtct
     6961 actctgatgt tggcgggaat caaagaaatt ctgattattt caacgccaca agatacacca
     7021 cgtttcaagg aattatttgg aaacggaaat gatttaggac tgcagatcga atatgcagta
     7081 caagaaagtc cagatggatt ggcacaagcg tttatcattg gagaagaatt tattggagat
     7141 gatagtgtct gcttggtatt aggggacaat atttactatg gtggcggtct gtctaaaatg
     7201 ttgcaacgag cagcttcaaa agatacaggc gcgacagtat ttggctatca tgtcaatgat
     7261 ccagaacgct ttggtgtagt agaatttgat gagaatatgc aagctttgtc gattgaagaa
     7321 aagcctgctc agccaaaatc aaattacgct gtaactggcc tttattttta tgacaatgag
     7381 gtaatttcta tcgctaaagg tatcaaacct tcagaacgtg gagaactaga gattacggat
     7441 gtcaataaag catatttgga taaaggaaaa ctatccgtag aacttatggg acgcggattt
     7501 gcttggcttg atacaggaac acatgaatca ttgcttgaag catctacctt tatcgaaaca
     7561 attgaaaaac gtcagaactt aaaagtggct tgtttggaag aaattgctta ccgtatgggc
     7621 tacatcgata aggaacaatt gctggccctt gcacagccat tgaaaaaaaa tcaatacggt
     7681 caatatttat tgaatttggc cgctgaataa ggagtcctta cagatgaaag taacagatac
     7741 aaaattaaca gatgtcaaaa tcgtagaaat ggacgtcttt ggcgatcatc gcggtttctt
     7801 cacagaaagt tattcaaaag caaaatttgc agaacatgga ttgaaatatg actttgttca
     7861 agacaaccat tcactatcag ctgaagctgg tgtccttcgg gggcttcatt tccaaaaagg
     7921 agaagctgca cagacaaaat tgattcgggt agtcactgga gcagttttag atgtagtagt
     7981 ggatatccgc aaaggtagcc cgacttatgg tcaatgggac ggatttatct tatctgaaca
     8041 taatcatcga cagttgcttg tacctaaagg atatgcccat ggattcgtta ctttgacacc
     8101 aaatgtgaac ttcctttata agtgtgataa ttactataat gcagaagcag atgggggcat
     8161 cgcatttgat gatccagatt tagcgattga ttggccaatt gatccatcaa gagcgattat
     8221 gtcggaaaaa gatcaccgtc atccgacatt aaaagaattt gaagcagaaa atccatttgt
     8281 ttacggagaa atctaagaat taaggagaaa ttttcttatg aaaaacatca ttgttaccgg
     8341 aggtgccgga ttcatcggct ctaatttcgt tcattacgtg gtaaacaatc atccagaagt
     8401 gcatgtcact gttttggata aactgactta tgcaggaaac aaagagaact tggctggact
     8461 tcctagcgat cgcgtagaat tggtcgtagg agatgttgca gatgcagaat tagtggaccg
     8521 tctagtaaaa gaagcggacg cagttgttca ttacgcggca gaatcccata acgataattc
     8581 attaaaagat cctttcccat ttgtacaaac gaatatcatt ggtacgtata cacttttaga
     8641 agcttgccgc aagtatgatg tacgctatca tcacgtatct actgacgagg tttacggcga
     8701 tcttccatta cgtgaagacc tgccaggaca tggggaaggc gaaggagaaa aattcactgc
     8761 ggaaacacca tataatcctt caagtccata ttcttctaca aaagctggtt cagatctgtt
     8821 agtaaaagca tgggtgcgtt cttttggctt acgtgcaacc atttcaaact gttcaaacaa
     8881 ctatggtcct tatcagcata ttgaaaaatt tattcctcgc caagtaacga atatcttaag
     8941 cggtattcgt ccaaaattat atggcgaagg aaagaatgtt cgtgactgga tccatacgaa
     9001 tgatcattct tcagcagtgt ggctgatttt gacgaaaggc agaattggcg aaacctatct
     9061 gatcggtgca gacggtgaag aagataataa gaccgtgatg gaattgatct tagaaatgat
     9121 gggtcaacca aaagatgcgt acgaccatgt aaacgaccgt gcaggacacg acttgcgtta
     9181 tgcaatcgat tcgacaaaat tacgggaaga gttaggatgg aagcctgagt ttacgaactt
     9241 ccgtgatggg ttggctgaca cgatcaaatg gtacgaagaa catgaagact ggtggaaaaa
     9301 agaaaaagag gccgttgaag cagcatatgc caaaaatgga caataataca gagaaaatgt
     9361 cactgtatta atggaaaaac acatgtgagg caggcgctcg cctgtctctt ttttaaaata
     9421 aaaatcaagg agcggataat catatgtttt tattaactgg aggaaatggg caactaggta
     9481 cagaattgcg ccatttatta gatgaaaaaa atctgcaata tgtttcaaca gatgcaaaag
     9541 acttggatat cacagatgcc gagaaaacaa tggcttacat cacagaatta aaacctgatg
     9601 ttattttcca ttgtgcagct tacacagcag tagataaagc agaagaagaa gcaaaagaat
     9661 tagatgagaa gatcaatgtt gatggtacaa gaaatgtagc acttgcagca aaagctgtga
     9721 atgctcaatt cgtctatatc agtacagact atgttttcga tggaaaaaac aaaaatgtag
     9781 aatacgaagt agacgaccaa acgaatccac taaatgaata tggtcgtacg aaattattag
     9841 gcgaaaaagc agtagaagag attttagaag actattatat catacgaact tcttgggttt
     9901 tcgggatcta tggacacaat ttcgtcttca cgatgcaacg cctagcggaa acccgcgaca
     9961 aactgactgt agtaaatgac cagtttgggc gaccaacttg gacacgtact ttggcagaat
    10021 ttatggtata tgtcatcgaa gaaaaagcac catttggtat ttatcatttg tcgaacgaaa
    10081 atagctgttc ttggtatgaa ttcgcaaaag aaatattgaa agatacagat gtagaagttg
    10141 cacctgttac gtcagaagaa tttccgcaaa aagcaacacg tccgcagtat tcagtcatga
    10201 gcctgaaaaa gacagaagag ttaggatttg tcataccaac ttggcaagaa gcacttgcgc
    10261 aaatgctgga agctgccaaa aaaatgaata agtaatttcc ttttatcaaa tagaatttgg
    10321 agcatgatat ggaaaaacaa acaagaagtg cacgacgaaa agaaaagcca aaagcgaacc
    10381 aggcagattc gaatttggtg cttgacctgt ttctaaaact ccgctatctt attggcgttt
    10441 tagtcatcgt ggtcatcgtt acttttaatt taaacggaag ttcacttggt gtttgggata
    10501 aatatgtgtc gcaaagagac gatgggaaaa aaactgatgt gatttttgga cagaatcggg
    10561 aagtgcggtc agacgaatgg ctggtgcaga caccttttta cctctcacaa gctgaagagg
    10621 actttccgtt ggtgaacgag gattattcat taaacggaca aaacatgatc attgcgtata
    10681 attctcctgt aaaggatatt acggttatcg gaaaaccatt caactgggga ttctttttcc
    10741 tcggaagaga tcgaggactt tccttttatt gggcgatgaa attagtcgtc atggtcttgc
    10801 ttggattcga agtactgatg atattaacga agagaaaaaa agctctatcg ctaatcggtg
    10861 catttgcgct gacattttct ccagcagtcc agtggtggtt catgcaacat gtgggcgatt
    10921 tgattttctt tactttggga ctgatgactg ctttttatca ttatttctac aatcatgata
    10981 aaaaatggct acgaacgttg atgatgcttt tggtggttat tttcggtatt gggtttattt
    11041 tagtgattta cccagcccat caagtgatgc tggcgtactt gctgattttt tattttgttg
    11101 gcctgcttat ttactatagc aagaaagttt catgggatct gttggatgta gtgttgattg
    11161 gaggggcagt tttatttatt ggcggagtca tggttcattt ctggctaacg tcaaaagacg
    11221 cattgttagc ttctttgaat accttatatc caggaaaacg ggtaagtact ggaggaaagt
    11281 ggacgattgg cgaatttttt tctttcctta caaattggaa aattcctttt gaagatatca
    11341 actattcgaa taactctgaa gtggccctgt tctatcactt ttttccaact gtattcctag
    11401 caagtccatt tgtgttattt ggcaaaaaag atagcgaaca aaagttactg ggacgtattt
    11461 tgatgatttt ttgtctgttt tctatctttt ggattaccgt tgggttgcca aaatggctag
    11521 cagaaatcac tctgctgtct tatgttcctc cattaagagc catccttact tttagttttg
    11581 cggcttgttt attatcgatc tggtttatcg cttatatttg gagagaagat gttattccta
    11641 tttggtataa attcattttg ttggcagtaa atgcatggtc ttatttctat gcgattacgc
    11701 attcgaaaat ggataattat ttttcggatt ttgaaaccat cgcagttgct gtgttgggaa
    11761 cagtgatttt agcttttgca ttatttggct ggagacgctt gttttacctc gcttttgccg
    11821 ccttagttat cgtatctggc tttttcgtca atccagtcgt agaaggtaca ggagcgattt
    11881 ttgaaaaaac actggcaaaa gaaatccaaa aaatcgatca acaagatcct ggtaaagttt
    11941 ggttgtctga agatgaatta tataacttca ccccaacatt gggagtgaaa tccttcaata
    12001 cagtacgctt ttatccagat atagctgcat ggaagaaaat cgatccgaaa gaagaatacg
    12061 agaagtatta taatcggtat gctcatacaa gagcttttat cgctaaggaa gatacaaagt
    12121 tcattttaga tcgtccagat atgttttcgg tcacgttgaa ttttgaagac gcagaaaaat
    12181 tagggatcga ctatgtgatc agtaaacgac cattaactga ttacaaccaa ttatatgaag
    12241 cgcagttcac gcagctatat ggaccggata aagacggtta tatgattttt aaagtagaat
    12301 atccggatta tccagatgta ccacaaacac aaactcagga aaatcagcta gtcgtacagt
    12361 aataggctag ctgagaataa tcctgaaatg ttagtagaaa ggagagtgga gataattcaa
    12421 gcaatcgtac aaaaatttgt caacaaaatg agtgtcggtt tcttgctttt gttactgacg
    12481 ctagtctttt ttgtttggac agcagggtta ggttttccag caactttgtt tatatataac
    12541 aatagttcat tacttatcgc ttttagtgtg gctattttgc tcattttaaa tgtttataaa
    12601 gcaaaaggcg tcgattttct ttatctaggt gttgcgcttg cccttttcat tttcttttct
    12661 tactttcggg aattaagaaa tgagtcttta tttggagact tactgtttcc tttgattatt
    12721 ttattttgtt tagtaattaa atggatcgag tggacaaaat gggatcgcgg tatttatctg
    12781 attgtctttt cggtcttcct cggaatcacg ttatatcgtg tatttacaga gatccaagta
    12841 ccagaaggac aaagcatctg ggctccaggc aattcgcttt ctgatatttg gatcaatacg
    12901 aatataatcg gaagttcttt gatgactctt gctttattga tcagtggatt tgccagttct
    12961 tttgaacgat ggtatgtacg aatcatcggt gcagtagcag ttgttgcagc ttttctcggg
    13021 atttgggtct gccaatctag aggtgcatta gtcgcgttga ttgtttttgc gatattggat
    13081 cttttaccta aatcgttgat gcgtgtcatt cgtgcgccat ttattgctta cacggttacg
    13141 attcttttag ctttgccgat ttcttatctg gcggctgtct ctgaaaaagt caatttgttt
    13201 accggtcgag aagatatttg gcataaattc tatcagacgc taggagaaaa atctgaacaa
    13261 attttactag gaatgaaaac atttatcttc caacgaggaa accaattttt aggaaaccac
    13321 aatagttata attctatttt gaatatctat ggattgatag gatttgggat tgctgctttg
    13381 ctgcttatcc tttttattgg tcgcttaacg ttgaaagcag atttatccaa cgggcagatg
    13441 acttttatct gggccttttt tgcggtgatg atgcagtcat ttatggagga tacattgact
    13501 tcatacccat gggttccgat tatttatatc ctgttggcta tggccgttca tcgatatgat
    13561 gaaccagaag ttgcggagga attctatgtt atggaagagg catatgacga tatgacttat
    13621 gatgatacat cttatgagga acaaccagat agcgctcatt cttccaggat ccaacgctat
    13681 cattgatagg cacaaaatac gagagaggct gtgacaaccg tcgcagcctt ttttggttta
    13741 ttgcaattgt ctagcatctt ctgtaaaata gaaacgttag agaaaatctt gtatcataga
    13801 aaattaagaa agaaataaga acgcagctcg gtaccaaaaa tatggtaaaa tggcacttga
    13861 ctgtaattca ctgaaaatcg cttggaggaa cacaaatgtt taaagatttg tatttattta
    13921 tgaaggacat ttatcagaac agaaaattat tgctacagtt ttcgatcaat gacttcaaat
    13981 caagatatgc aggatcgttt ttaggaatct tatgggcgtt tatcaatccg ctatttactg
    14041 tattgattta ttggctggta tttggtgttg gactgaaagc acaaatgatc gatgcaaaat
    14101 acccatttat cgtatattta gttaccggga tgattccctg gttcttcttc tcagatgcat
    14161 ttgcatcaac tacggttgtt tttcgggaat atacttattt agtaaagaaa gtcgtattca
    14221 atattcgtat cttgcccacg acgaaaatcc tatccaatct gtacacccac ttattcttca
    14281 ttctgattgg catagctgtc gcgcttataa atggtatata tccttcagca atgtctttac
    14341 agctgattta ttatttattt tgcatgtgtg ctttcttgac aggattgact tggctgacgg
    14401 catcgatcca accattttta cctgatatca tgcaattaat tacgatcgtg ctgcagttga
    14461 tcatgtggac attaccgata ttatggagcc ctaatcagtt tgattcaggt cttgtaagca
    14521 tattgaaatt aaatccgctg tactatgttg ttcaaggtta tcgtgagtca tttttaagtg
    14581 aagcttggtt ctgggagcat ggaacatata ctttgtattt ctgggttttc acgttagtaa
    14641 tgttcgtaat cggctctgca gtgttccgta gattgaaacc acatttttca gatgtgctgt
    14701 aaggagaata gctatgacag aatatgcaat tagactaaaa gatatcacaa aatcttataa
    14761 tatgtacgca aaaccttcgg atcgatttcg tgaagccctc aatcctttta agaaatccta
    14821 tcatgatgta ttttatgcgt tgaaaaacgt gaatgtagaa ataaaaaaag gtgagatgat
    14881 cggtttcgtg ggagaaaacg gatcgggaaa atctacaatg ttgaaaatca ttacgggagt
    14941 actgacacca acatccggca cgatggaaat cgaaggaaaa atctcagctt tgctagaact
    15001 aggatcagga ttcaatcctg agtattctgg ctatgaaaat atctatttaa atggaatggt
    15061 tctaggattt tcaagagaag aagtcgatga aatggtcgat gatatcattg aatttgctga
    15121 catcggagac catatttatc aacctgtaaa aacttattca agcgggatgt tcgttcgtct
    15181 agcttttgct gtagcaatca atgtgaatcc cgatattttg attgtggatg aagcattggc
    15241 agtaggcgat cttgaatttc aactaaaatg tatggaaaaa tttacagaaa tcaagaactc
    15301 gggaaagacg attttattcg tttctcatga tatcagttcg attcgtcgtt tttgtgatcg
    15361 ttcctattgg ttgaaaaacg gagaagtcgt cgaatatggc gatacgatgg aagtaacgac
    15421 gaattatgaa aactttttaa agaaaaaatc agttaaaaca gtggatcgga ataaaaacca
    15481 agtagaacgg ttcgccgctc cagatatcgt cgacgtgacg aaagcggaat tgttggataa
    15541 agacaaaaat ccggtggata tgctggaaca aggagaaaaa ctatacgtta aagtaactta
    15601 tgaagtaaaa gatgatacaa tcaaaaatcc ggttttagga attgcgctac gaacagtcga
    15661 caatttttat gtatgcggac ttaatactct actagatggt atcaagattc cttggaaaaa
    15721 aggagagaat atcttttatc tggagtatga aaagttaggt ttgttagctg gtgaatatta
    15781 ttttgacgtt gcggtctttg aagaaaatgc aacggttcct ttggtatata agacaaagta
    15841 catgaacttg tttgtcagcg gttcttatat cggagaaggc atcgtggtct tagaccataa
    15901 atgggaagaa gggactcata gcaatgaaat atgattttga aatggaccta gatgaacaaa
    15961 gcagtgtggg aaaaattgcc gcacagatca aaccaggaag taaagtttta gagtttggtc
    16021 ccggaaacgg ccgattaacg aaacatttaa tcggagcaaa acaatgtgaa gtgagcattg
    16081 ttgagttaga taaggaattg ttcgattttg tcagcgaatt tgctcaagat ggattttatg
    16141 gtgatatcga aagttttgag tgggcaaact attatgctgg acagacattt gattacgtct
    16201 tgtttgcgga tgttttagaa catttggtag atcctggaaa aacattgaag aaagtcagag
    16261 aatttttgaa tgaagaagga gagatcctga ttacattccc taacttggcg cataattctg
    16321 tcatgatcga tttgttcaat aaccaattgc cgtgggcttc ttatggtctc ctagatgaaa
    16381 cccataattc tttttatact catgatggct tccaaaaagt ttttgaaaaa gcgggattat
    16441 tcattaatat tgaagattat ttgtacttag ctgtaggtga tacagaatta aaatcaactt
    16501 atgaagaatt gccggaagct gtacgctatg atttcaaaat gcgaccattt ggtgaagtct
    16561 accaatactt tttctcattg atgaaacatc cagtagcaca aagttccatt gcagagccac
    16621 aaaattctaa ttatgtaaaa gtgttagaag taacccagca aacgaaacaa gatgaaacaa
    16681 tccaacaaat accattcaat aatttcacag gggaaaatga gacactaagt ttccccgtat
    16741 cagaaacaac agaaagaatg gtgtttcgct tcgcgcaaca acctagcttt atcgaattca
    16801 gcgcagaagc tgccggagaa aaactaacat ttatcgattc gaatgctgta gtcaaaacag
    16861 taaatgattg ttatttgttt gatgggaaag aattaccaga gttcgttcta acggatattt
    16921 ctgggaaaga agtgactatc cattgtcact atcgctttat cggggaattg acaccaacaa
    16981 tgaaagaatt gttggaaacg attcgtccca tggctgaagt cacaaagcag ctttctgaga
    17041 aaaatgacga attagaaaga gaaaatcatc atcttcttga ggaaaataca agactagacc
    17101 atacgttaaa aacaaccaca aatcgctatt gcacattgtt ggaaagtgat gaatggacaa
    17161 taaaacatag actaggacga cgtaagaaag aaacttcgaa aaaaatccaa gaaaaagaac
    17221 tttctatatg tattgacgag aaaatctggg atgcagaaac aaaaatatta aaaatcatcg
    17281 gatggggaat cgctaattcg gatcgccagc ccttgtccta taagttatca gctgaccaat
    17341 cgccattttt cgaagcaatt cctgtttcac gagaagaagt caatcaagca gaacagctcg
    17401 caaaaggaac agaagcggga tttgagttac gaatcctttg cgagcaagaa cgttcattct
    17461 tagtggaagc agtagctcaa aatggccaat catggatcat tgaaatgtaa ataatgatta
    17521 acttataaag ataaggagta aacgaatcgt gcaaaaaaaa gaaattgccg tatttatcga
    17581 tcaaatcact cgtgaacggg ccactggcga tttgcttatt gtgggctggg caattgatga
    17641 agtgaccaaa gaaatcccta cgataaaggt agaaaaagaa aatgtgattg ctgaagcaac
    17701 acatgtcgta agattggata tcaatcattt atataatctt gatgttaaga cccaaagcgg
    17761 ttttaaggta cgtttatcag ggaaaatgag aggaaaagcc atcttagact tccaaacagc
    17821 gagacatcaa aatggcatag ctgtgaaatt aaacggtaaa tacccttatg atgacggcat
    17881 tgaatcttca tgggaaagaa aaaaaagatt actgaaaaaa gggataaatt atgcaaggac
    17941 tcatggcgta aaaaaagcga tccgtcgggt caaattggaa ttgaagccag gatcgatcga
    18001 ctatgcagag tggatcagtc gtcatgagaa accagatttg aaagagcaaa ggaaagaagc
    18061 acaaggattt gcgtaccgtc cattgatttc catacttatg cctgtctata acgttgaaat
    18121 caaatggctc gaaaaatgca ttgattcggt actggatcaa acgtatgatc attgggaact
    18181 atgtatcagt gatgatgctt ctactgatcc agctatccga aagtgtctgg aatcctatca
    18241 agcaaaagat gatagaatca aagtcgtttt ccgaaaagaa aatggacaca tcagtctggc
    18301 aactaattct gcattggaaa tagcagaagg agaatttata gcactcttag ataatgatga
    18361 tgaattaccg ccatttgcat tatatgaagt ggcaaaagtg ctaaacgtac atccagaact
    18421 ggatgtgatc tacagtgacg aagataaaat cgatgcagat ggcaatcgct ttgatccgca
    18481 tttcaaagca gactggtcgc ctgatacatt gatgggaaat aattatatct ctcacttagg
    18541 cgtatatcga acaagcattg ttaaagaact tggcggtttc cgtaaaggct atgaaggttc
    18601 tcaagattat gatttggtcc ttcgtgtgac agaacaaatc cctgcagatc atatttacca
    18661 cattgaccga gtcttgtatc attggcggac gatcccagga tcaactgcaa gtaatggcga
    18721 agccaaaagt tatatctatg attccggagt gaaagcattg acagatgccc tgtctagaag
    18781 aaacatcaaa ggaagcgtac gtcctggcag aatttctgga ttttatgaga ttgcttatga
    18841 cgtcttgcaa gaggatttgg tcagtgtgat cattccaacg aagaatggct atgaagatct
    18901 aaaaacttgt gtggattcga tcatcgaaaa aacgtcttat tcaaattatg aaatcattat
    18961 tgcggataac ggaagtacag atccaaaaat gcaagaatta tttgcagaat acaagcatca
    19021 gctgaaagac cgctttatcg tagaattgat tgacattcca ttcaattatt ctcgaattaa
    19081 caatttagca gcagaaaaag caaacggcaa atacttttta ttcttgaata acgatacaga
    19141 agtgattgag ccagattgga tgacggtgat ggtttcttat gcacagtttg atcgaatcgg
    19201 ctgtgtagga gcaaaactgt tttatccaga tgatacaacg cagcatgcag gagtattatt
    19261 aggaatcggt ggtgttgcag ggcatgcatt gaataactat gatcgaacac attgtggtta
    19321 tttcggccga ttggtcattg acgtcaatta cttagcagtc actgcagctt gtatgatggt
    19381 caaggcagct gacttcaatg ctgttaacgg gtttgatgaa acattagaag tagcattcaa
    19441 cgatgttgac ttgtgcttga aagtttatga actgggtcgt tacaatgtct atgcacatca
    19501 agcagaattg taccatttcg aatctaaatc aagaggttat gaagatactc cagaaaaaca
    19561 aagacgtttt gctggtgaaa tcaaaaaaat gcaagataaa tggccagcat atattgcgca
    19621 tgatccattt tacaatgata atttaacaaa agaaggaatc ggcgactttt cgttgagacc
    19681 agattaaact ataataaaaa gctgttatgt tgtgattcgc tcgcacataa cggcttttta
    19741 tagttttttg tcaaaaaaag gcagaaggtg ccgactcaaa aaaataagtc gaggttggga
    19801 cagaagtgtt taactccgag aaataagaag aaattcacga aaattgcttt tcaaattttt
    19861 gtgaatttcg gcttatttaa gaagaagttg cttctgctcc caccgtttat tcgtttttaa
    19921 agaatgagac aaaagtgttt cttacttttg tcccacgctc ttattcatgc tcagagcgcc
    19981 aatagataca aaatcatagc caaagtttca cgaaccctag ctgtcaaaga atcgatattt
    20041 tattttaata taggctattt taaaataaat ggtttaaggt attcctctac aaaagccact
    20101 ctatcaatta tcatgggttt aaaataagaa gtgatggaga taaccagatt gttcttagcg
    20161 tcaatataaa gaacatttcc actattacca attgctgaat atatttcgtt ctcagaatct
    20221 aatacccacc ataaatagcc atatctcata ttttgtaagt cttctaaagc actggttcta
    20281 ggtttgaaca tctcgtttat ccacatgggt ggtacgattt gttttccttt atacatccct
    20341 ttatttaggc ataatacacc aatttttgcc agatcacttg ccgataaaca caaaccatat
    20401 cctgccgtcc cgatatttag tggatcacaa aaccagacat tggttttagg agacttatcc
    20461 attgtaaact gcttatgttc ttcagctgtt tcagcataat aattgacatg gtttggtatc
    20521 cctataggat caaatagata cttattggca aaatcaacag ttgttagctt acttgtctga
    20581 tacaacaagc cagttaatat gtgtaaacag acagtttgat agttgaattc gccggttata
    20641 ccttttcttc ctcccaataa atctagtgat gtttttgtcc aatcttcact tgaacagact
    20701 tttgtccatg gatcgccttt acatttgtag ggtgctttca ttgttagcaa gtgcttgatt
    20761 gtaatagcgt aaatagtttt ctccccgcgt ttcactttat actctggaaa ataatctaat
    20821 actttatcat cgatactttt gattaatcct tgctctaaag caatgccaat cagcaaagat
    20881 acaatacttt tcgttgctga catgatatga caagtatcgt cttttttata accattccat
    20941 tcatctgtat aaacttctac cccatcctta tatgcgacaa tttgacatat atttggttgt
    21001 cgaaaaccaa taaaatcatg caactcttgt ttattcataa gatattacct cactttcttc
    21061 tataagcata atgtcattaa aaatcgattt caattttttc gattcttcac gtaatacagg
    21121 tattcttatg gactagacag cgctttggga agatggattc tctgaaaaat ataaaggttc
    21181 actttatgag tggataaaaa aagcagcaaa tacttcgact tctttaattt accaacatta
    21241 gtgaaaatcg attcatctgg agatttgtaa tttagagtcc ttgtcttgat tagaggtgta
    21301 taaagctttt tattagacat ttttaaattc cattacaatt tgcttataaa ctaggttttt
    21361 aaaggtgact atggtaagat aatagagata ttattttgtg attaggagtg tatatatgaa
    21421 taaaaatgga gaatggagtg ctgcacgacg gatattcatt atattaatgg atgttattgt
    21481 atacaatgca gcaatatata gtagtttctt actgaagttt cgtggagaga tcccattacg
    21541 aaatttagaa actttccaac actcagcaat tattatttca gtgattttta ttgcgttgaa
    21601 cattttgttg ggtgcatatg ttttttataa tcgaatgatc agtgacatcg tgtttgtcac
    21661 agtcatcggt caggtattga tgacactagg gatcatggta gtgacttttg cgggccgctg
    21721 gtttgctttt ccacgtcttg ttatcttact ttcatttgtt ctcggtacgc ttttcttaag
    21781 tatctatcgt attctcgtat ataaacttta tttacgcgta agtcgagata aaaaggtcgt
    21841 cattattggg ttagaaaagg atgtcgcacc agcaatccaa aattttggtt caaagaaaaa
    21901 caacaaacat aaagtcgaag cagtcattct tgataactat tatgcaaaca tcaaaaatat
    21961 gattaaagac atcgatattg tctatcttgc ttctcatatc gaagaaactg aaaaaatcaa
    22021 aatctatcaa ttagttacta aaaatgaaaa aaaattgttt ttgaatacaa catttgaaaa
    22081 cttgacattg atcaatccaa atattatgaa tttcgaagat gaaagtatta ttgaagcatc
    22141 tggatttaga atccaacctg aatatgaact attcaaacgt ttgtttgata tcatcgtttc
    22201 acttgtttta ttagttattg cttcaccatt tatgttattc acagcaattt tagtcaaagt
    22261 gacatcaccg ggaccaatca tctataaaca agtgcggatc acaaaaaatc aaaaggaatt
    22321 ttcaatctat aaattccgct cgatgaccgc cacagccgaa gcaaaatctg gaccagtttt
    22381 agctaagagc aatgatgcac gagtaacgcc tgtaggtaaa tttattcgag ctgtccgctt
    22441 tgatgaatta ccacaaatct tcaatgtgct gaaaggcgat atgtcgatcg taggaccacg
    22501 tcctgaacgt cctttcttcg tcgatcagtt caatgcagaa aacccttatt actacttacg
    22561 gcataatgtt cgtgctggta tcactgggta tgcacaagtc tatggaaaat atgtctctga
    22621 ttacaacagt aaattacgtt ttgatttact ttatatcaaa aaatattcat tgatgcttga
    22681 tttaaagatc ttgctgcaaa ccatcaagat cctatttgat aaaatgtcct ctcaaggact
    22741 tgaagaagac gaaaatgtga tctcattggc agaaattgct tttccgaatg aaaaaatctt
    22801 tcgctagttg ggaggcgtaa aaatgaacaa tccgaaattc agcataatcg taccagtata
    22861 caatgcagaa gcaactattg gtcgaacgat tgatattcta aaagaattgg attatgaaga
    22921 gtacgaagtc gttttagtaa atgatggatc aacagatcaa acacaggaaa tcattcaaga
    22981 actgattgaa aatgatgaac gttttgaatt gattactacg accaatcaag ggcccggatt
    23041 agcaagaaac gaaggaataa gacagtcaaa aggagaatat ctcttattct tcgatgcaga
    23101 tgacacgcca gtaaaaacga tcttgtcaga ttatgatcat ttgctagaaa agagtccaaa
    23161 agcggatctt gttgttagtt cgtttgtttt tcgtacaatg gacgagggaa gaatcgtttc
    23221 cgaaaaagaa aacttagtta cagaatgtca ctatctaact cacgaagagt ttataaaaga
    23281 catgtatcag ttaatgaatg aacagttgat gtatgttgta tggaacaaat gctatcgtag
    23341 cgatattctg aaaaataacc aaattctatt taaaacttat aatagttgtg aggatcgaat
    23401 atttaactta cattattatg aatactgtca tcaagtgatc atgaacccaa aagtagaata
    23461 tatttatgaa tttgaaggtg ggaaggggat cactaatcaa taccgaccta acaagttcga
    23521 aacatttaaa gaattctatt cattagcaaa tactgtaaca gacgatatta ataaagacgg
    23581 tatggcggcg cttcttttaa aagggacaac ttcagttatt ttttctattt atagtacctc
    23641 tgcaagaact tcaaaagaga agaaagacga agcaaagcag atattgagtg atccagtgat
    23701 ccgagaagcc aagcaaattg ctaagacgga ttcttcagca aaaaaaataa caaagttgct
    23761 ttacaatatg ccagctccac tatttttatc tgctgtaaaa gttggaagct tcgttgaagt
    23821 gaaaatgcca gggttaatgg ctgctttgaa gcgagtatat tagttaatgg aggaaaatat
    23881 aatgaaaaga attttgcatt ttcagggacg aatgggttta ggtggagcag agtctttcat
    23941 gatgaattta tatcgtaaaa ttgatcgtac aaactatcaa tttgattttt tgatttatga
    24001 agattatgca gatgttcaag actatcattc agagattgaa agattaggag gccgtatttt
    24061 tgttgtacct aatcctaaga aaaatatttt taaatattta atagaagtga ataagttact
    24121 aaaaaaagaa tctttttcta tagttcacaa tcaggtttat tttggcgggg gaattaattt
    24181 atggttagcc aaaaagaatg gaatcagaca gagaattgct catagccatg caactgaaga
    24241 tggaaaatct caaaatattg taatgaatgt attgcgtaaa tttttaacca aattgttgtt
    24301 acaaaatgca acagactacc ttgccgttag tcaagaagct ggtgaaagtc tgtttcaaaa
    24361 ccaccccttt gagatagttc ataatggtat tgatttagag ctttatagta aaaattcaga
    24421 agctaaggtt aataagagaa aagaattgga tatctcaatg agtacttttg ttgtgggaaa
    24481 cattggacga ttagagaaac aaaaaaatca atcttatttg ttagagattt ttttagaaat
    24541 actaaaaatg aattctgatt cttacttgat tttagtaggt gaagggtcac ttagaaataa
    24601 actgaaagcc aaagcagtag aattggatat agagaaaaga gtaaaatttt taggagaaag
    24661 aaacgatatt cccgagttat tatctgtgat ggatgttttt gtaatgcctt ctctttatga
    24721 aggtctgcca atggttggat tagaagcaca agcagcaaaa ttaaaattag tcttagcaga
    24781 tactatttcc aaagatacaa agttaacaaa taatgtggat tttgtatcat tagatgaaac
    24841 accacaagaa tgggcaaaaa aagtaataaa agagccgtat gctaataaaa taacacaaga
    24901 actaaagagt tatgatgttt cgtacactac tgaaca
//
LOCUS       DAJSKS010000027        24843 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJSKS010000027 DAJSKS010000000
VERSION     DAJSKS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.028"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..384)
                     /locus_tag="ON396_000961"
     CDS             complement(<1..384)
                     /locus_tag="ON396_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113063.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKI"
     gene            complement(569..967)
                     /locus_tag="ON396_000962"
     CDS             complement(569..967)
                     /locus_tag="ON396_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR3113064.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(964..1737)
                     /locus_tag="ON396_000963"
     CDS             complement(964..1737)
                     /locus_tag="ON396_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCR3113065.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1734..2417)
                     /locus_tag="ON396_000964"
     CDS             complement(1734..2417)
                     /locus_tag="ON396_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCR3113066.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2584..3471)
                     /locus_tag="ON396_000965"
     CDS             complement(2584..3471)
                     /locus_tag="ON396_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3113067.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3468..4337)
                     /locus_tag="ON396_000966"
     CDS             complement(3468..4337)
                     /locus_tag="ON396_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3113068.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4474..5760)
                     /locus_tag="ON396_000967"
     CDS             complement(4474..5760)
                     /locus_tag="ON396_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3113069.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            6033..6611
                     /locus_tag="ON396_000968"
     CDS             6033..6611
                     /locus_tag="ON396_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR3113070.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6611..7540
                     /locus_tag="ON396_000969"
     CDS             6611..7540
                     /locus_tag="ON396_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3113071.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7537..9273
                     /locus_tag="ON396_000970"
     CDS             7537..9273
                     /locus_tag="ON396_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR3113072.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="ON396_000971"
     CDS             complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="ON396_000971"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCR3113073.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(10169..10678)
                     /locus_tag="ON396_000972"
     CDS             complement(10169..10678)
                     /locus_tag="ON396_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCR3113074.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="ON396_000973"
     CDS             complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="ON396_000973"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR3113075.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11979..13070)
                     /locus_tag="ON396_000974"
     CDS             complement(11979..13070)
                     /locus_tag="ON396_000974"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCR3113076.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="ON396_000975"
     CDS             complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="ON396_000975"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR3113077.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="ON396_000976"
     CDS             complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="ON396_000976"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR3113078.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="ON396_000977"
     CDS             complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="ON396_000977"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCR3113079.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(17033..18007)
                     /locus_tag="ON396_000978"
     CDS             complement(17033..18007)
                     /locus_tag="ON396_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HCR3113080.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17988..19871)
                     /locus_tag="ON396_000979"
     CDS             complement(17988..19871)
                     /locus_tag="ON396_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HCR3113081.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19889..22114)
                     /locus_tag="ON396_000980"
     CDS             complement(19889..22114)
                     /locus_tag="ON396_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR3113082.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            22229..23263
                     /locus_tag="ON396_000981"
     CDS             22229..23263
                     /locus_tag="ON396_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3113083.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(23330..24343)
                     /locus_tag="ON396_000982"
     CDS             complement(23330..24343)
                     /locus_tag="ON396_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3113084.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24421..24609)
                     /locus_tag="ON396_000983"
     CDS             complement(24421..24609)
                     /locus_tag="ON396_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR3113085.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7131 a         5402 c         4436 g         7874 t
ORIGIN      
        1 aatcttttgc tgacgtctaa aaatagaaaa agaattttcg tcatctattt gataacgcat
       61 ttgtttttgc agttctgctg cagtcagatg gatcttccca tcgtttgtat cgatcattaa
      121 accttctgga aacagttcaa ccactgctcg atcccaagat tcaagagaaa gctcgatata
      181 atcttttact ggtaattcca attctacctt aaaaaagtgc aaaacagccg acagcccttc
      241 tgcttgatag atttctccta atgttagccc ctctagatgg aactctggtg aaaaatgcag
      301 caatgtttta ttttcctgca attgaacata tagaaatgta gatcgctcaa tcagagtatc
      361 ttttaaaaca actaaaaaat tcatttttct cccccttttt tcatcatttt taaaatagca
      421 tattttgaaa agaaagtctg atgcttagag tagaatttca taaaaattta ctatttcata
      481 agtattcacg agaaaaatac aaacaaaaaa gcggaaataa cgctcatccg ccttcttgtt
      541 tgtgtttatt tcctcttcaa acgcttgtct atgattcaga cttttctcta tcgttatttt
      601 catctaaaag ttcttgcatt ccgataattt ctcgatattg atgaatctta gtgataccaa
      661 tgactttgtc tacatcaata ctaaaacaaa taccattacc tggaatatcg atgttgatgc
      721 cttgaccaat cgcatccatt actttattcg tcaaactatc gggtactaat gtcaacacaa
      781 tatctttttc tggcgaaatg tccaaaccta aaaatttccc tgaatcatgt acaccagaac
      841 ctcttccatg taagatcgtt ccgcctgctg cacctgcttc ttttgaaaag tcgataacat
      901 cttcgcccat tcctccgtca acgatcgtca caatcatgtg aagattcaat gctacaagat
      961 ctttcatttg ctttccccca atttttgcaa tctttctttt tcgattctgt ctttttcatc
     1021 ttttcgttta taaatatatc ctagtactaa agtggataat attggtgtca atgcaatcat
     1081 tgcaaccaca ccaaacccgt caatgatcgg atctcttcct tcaatttgtg cagcgattgc
     1141 cacgctcatt gataaaataa atgtcgaaca catcgggccc gttgctactc caccgttatc
     1201 aaaagccatt gctaaaaaga tcttgtccac ttttcgtcct aaaataaatg ctagcccgta
     1261 tcctgggatc aagaaataat aaagagagaa tccgacgagc aaacgccaca tagacaatcc
     1321 tactgctgcg ccgatcccaa ttgaaattac catcagcatg attttcgatc ggattctccc
     1381 ctcgctcaat tcttcaacct gtttgatcat tacatgaatc gccggctcag caaaccctac
     1441 taagaacccc attaaaaagc caaggggaac cagtatataa ttgtaaggca atcccgcaat
     1501 agcactgcct aaatgctgtc ctaccggtac ataagccata ttgactccgt gcaggaaaag
     1561 gatcaaccca atagtcgtta tcaagaaacc tttcataatg tcaaagaaac gctttttgcg
     1621 catacgaaaa ctaatgatat tcattaaaac aaataggatt acgataggtg ccactgccaa
     1681 aatgacttcc atggtaacga tttcgattcc atctatcact tgctgccaga aactcattgg
     1741 aagatcaccc ccaaaatcat cacggcaaga atcggcccta acgaagcaat accgaccatt
     1801 ccaaaactgt cattttcatt tttcttttgt ttgatcatac tagtcatccc tccggcaagg
     1861 gccaagataa atggaacagt tactggacca gtagtcactc cccccgaatc aaaagcaatc
     1921 ggcataaatt cggaactggt gaaaaatgag gcgatcaaaa cgcctatgta gcctatagcc
     1981 atcaattgat aataggatag cttgaatacg acccgaagca gcgcaaaggc tagaaatact
     2041 ccagtgccga cgctgacgat cccaatcagc aacactctgc caatctctcc gtcagatata
     2101 tcatatactt gttgtcccaa aacttgcacg gaaggttcag caatcgtgat cacgatcccg
     2161 atagcaaatc ccaaactgac caaaacggtc aaacttttct ttttgatcat atatttccct
     2221 actagatctc cgacttccat catagaataa tctgctccaa acaaaaagag cgtcattccg
     2281 atcatcatga ttgccgcacc aaccagaaat gaagcgaggt cttctccttc taacggtgca
     2341 aaaacaaacg ttaaaatgac aatcaggaca gtcatcggca atatagcagc aatcacttct
     2401 ttaaaatttt ttagcatgta tcctcctcct ttactcttta agtttacctt aaaatgaaag
     2461 agttttcgag cacaaactta aaacgttatc atttaatttt tctcaacata aaataacaca
     2521 cgtcccaaaa gtatcttttt acttggaaaa atgactttga aggaacgcgc ttattgtgtt
     2581 tttttatttt acagagccga tcattccttg aacgaattgc ttttgcaaaa caaaaaatac
     2641 gatggcagtt ggtaaagtag cgatgactaa tccagccatg atcattccat agtctggtgt
     2701 ataggaagca cccatagcag ataaaacaag cggaaccgtc cgtttatcag gagattgcaa
     2761 agctactaag ggccataaat aattgttcca gctactcatg aatgtgatga ttgcagccgc
     2821 ggcataggta tttttagccg tcggcatata cactctaaaa aaaatactta gttcttttag
     2881 accgtctaat cgagcggctt ctactagatc ttttggaaaa gatttaacat tttgtcgaaa
     2941 gaagaagatc aaaaaagcag tgctaacaga tggcaggatg acaaccaaga aactgttgat
     3001 tcccaaaacc gttccgttca attgtgaaaa cattcgatat aaagggatca tcaatgccgc
     3061 aaaaggaacc atcatcgata gcagtagaat attgaaaatt aggtcttttc gtttactttg
     3121 ataaatctca aatccatatc ctgccattga cgaaatcagc aatgcaaaca cagtggtcac
     3181 caaagcgatc atcgctgaat tccaaaggga acgagtaaaa ttcagctcat tggaaaacaa
     3241 attttgaaaa ttgatgatca agttactgcc aatctttatt tttcccgatg tgatatccac
     3301 gggtgtattc gtcattccca aaaccatcca gacaaaaggg aaaatggaaa taaatgatag
     3361 gatgcttaat gctgtatact ttaatactga cccaattgtc gaacgaagtt ctaatgcttg
     3421 ttttttgacg ttggggcgtt cgattgtttc tttttctacc atatttttca tcgactttct
     3481 cctcctactt tcgtttggat tatcgacaat atcacaataa aggaaacaat aacaaaggaa
     3541 actgctgccg catatccaaa attaggcgtg aatttataac tcaaattata gatgtattga
     3601 gatagggtgg tcgttgcatt tgctggtccc ccgccagtaa tattttgtac ttcatcaaaa
     3661 agctgcaatg tcccgatcgt cgaagtaatg gaagtaaaca aaatgattgg ttttaaattt
     3721 gggatcgtca ctttgataaa ctgctgccat ttagaagcac cgtcaataga tgctgcttca
     3781 taaatctccg aatcaatatt ttgcatgcct gaaaggaaaa aaatcatatt gtatccagtc
     3841 caacgccaag tgatcgaaag aatgatcaga actctcgccc aaattggatg agtcaaccag
     3901 ccaatcggtg tgtcaatcat accaatgccc aacaacaagt tgtttacaag gccagattga
     3961 gaaaatagac ttttcatgat cagagaataa ctgaccatcg aagtgataca tggaagaaag
     4021 atagcggttc gaaacaaccc tttatatttc aactttttat catttaaaag gttggaaata
     4081 aaaagtgcta gaaacaacat gatcggtact tgtacaagta aatacaggaa tgtattaaaa
     4141 aaagcttttt taaaagttgc atctgtcaac atccgctgat aattacctaa cccgttaaat
     4201 gtcatattga cagggggacc actttgaaaa gacgtgatca aagccaaaac catcggaata
     4261 aaaaccatga ttgatatcaa aatgactggc aagattaaaa aagcatgccc gtttttgaat
     4321 aaatctttct gcttcattct tttccctcca attctatcat tgattatcta catgaaaaga
     4381 caagaaagac aatatctgta aggcagacgt gatctttttt gtcttttcgt ttgtctgatg
     4441 aaaaagcgaa gaaattcact tttttaccac cgatcaatta gccaattgtg cttctacttg
     4501 tttttgtgta tctgctagta ccttgtctgt ctcttctcca ttgatgattc gatgaagagc
     4561 ttcagctacg actgcttcaa ttgcataggt ctcatgtccg taattcactt ctggtatttc
     4621 agatgtccat gttgaaaaat cgtcaaagat cttttgacca ctgtaaaatt cagaagcttc
     4681 ttgataagct tcttgttcat tcgcgctaag catcgtagat actagtccga tttctttcgc
     4741 aagagtgccc atcagttctt cattcgaagc aaacgttgct tctaggaaat ctttcgctga
     4801 atcttctcca gctactcctt tgatcacata ccagccagcg ccgccaaggt tagaagcttg
     4861 cgctttttgc agattttctg gaagtgcagg tattggtgca atcttccatt ttccagattg
     4921 gttttctgct ccttggatcg ttgaagaata ccaagcgcca gttgggctag aagctactgc
     4981 tcctgattgt atcgcagtta ctccagcatt ccagtcagag gtttgttcga ctagatcttc
     5041 tttcaaaaga gtggcaaaca attcaagacc atactttaaa gatgcattgt cttggatcgt
     5101 gactgtctcc ccatcttcag atgtatacca ttctcctgcc tgttgcataa tcatccttac
     5161 tcgaccaagg tcagaaggat tgacttcagc gattttcttt cccgtctttt ctttgacatc
     5221 tcgcgctact tgaatatact cgtcccactt cagattctcc atatcctctt ctgtataacc
     5281 tgcttctgcc atcagatccg tacgatagaa gtttgctgtt acgcccgaat cgaaaggaac
     5341 accgtatatt ttatctccta ctttgtttac cgcaaattta tacggtgcaa aatgatcttc
     5401 ctttacaata gcagacaaat tagcgaatgc atctggataa gaagtcaaat agccttgtat
     5461 cctataatct tcaatcaaga cgatattggg caatccttct gtattcccgc tcgctaaagc
     5521 ggtgttcagt ttctgaacga tatcatcctg agacatcgta acaacttcta tctctgtttc
     5581 ttcgttctca taaacttttt ttgcttcatt cactgctttg atattgaatg tctcatccca
     5641 cgcccagaca gtgacttttt cttctttgtc tgctgtacta ctagcacttc cgcatcctgt
     5701 taagaaacct gatgctgccg ctaaacaaac gactccagca attaactttc tgatgttcat
     5761 ttttatccgc tccttttcca tccattagtt gtttacgttt gctacattca tcataccaaa
     5821 atttgaaaac ggtttcttta ttattcagat acttcaaaaa tttctcctaa aataataaaa
     5881 aaatacaaat caactatgac aaaaggaata atcttagcat ctagaccaaa gatcgaaatg
     5941 gtgaggtttc tttaaaataa aaatatcctt gcattttctc ctagaaataa gtatttttaa
     6001 attacaagac tattcttaag gcgggtaaaa aaatgccaat acgcgtaatt cttatcgatg
     6061 atgaacagac gattttagat ggaatgaaac agttgatcaa ttggtctgag cacgggtttc
     6121 aattaatagg gactttttcc agtccgctga aagcgcttga tttttctttg gtacatgcaa
     6181 tcgatatcgt ggtcacagat gtaatgatgc ctgaattaaa tggaatcgaa ttatcgcggc
     6241 tattaaaaca gcaacaacct gaaacacaga tccttatcct ctctagctat gatgaatttg
     6301 atatggttaa agattctttt aaagaaggcg tcgctgatta tcttttaaag cctcgattga
     6361 gtcctgattt ttttctaaat tccttaactg ccgtttcaca aaaaatcaaa aaaaagccca
     6421 acaaaagccc aatcaccaat cgttatgagc agatctgcga gaatctttca cagcagattg
     6481 ctggacaaac tcgtccattg cttgaccctc ttctttttga acacaccttg tttttctgtt
     6541 atataccgaa aaacggccat tcagttcaaa acaaggctct aaacaaaaac agtcgatcac
     6601 ccattcattg atggatagcg acgattcgtt tactatttat cctgtttcta ccttttcgga
     6661 agaatccggc tatgtaatca atactgattc caaagataca cttaggaagt ttgttcaaaa
     6721 gtttcgtcaa gaagtttttc caggtgattt ttttgttgtt tctgattctt ttgcaatcca
     6781 agatatcaat gaaatattcc aagagatcag aaattgcacc aaaggacaac gtttctatca
     6841 taagggaaag aaccttgctt atcagcatga acttcttccg ttattaacca accacgagca
     6901 gcaagcaagt agttatttaa caaaaatcgt tcataaagac tacgctttag ctgtcaaaga
     6961 acttgagaat ttcttcacga cgatattgga acagcctatt gatccagctt atttgaaaca
     7021 tgaggcaatc aatcgtttct atgcattact caacgctttg gctgacgagc atgttgggaa
     7081 tgaagaactt tctttgctca agatcgccct tccttcttta ttagccgatg ctgagtattt
     7141 cgaagatttt tctattcttg tcaaagaaac acttgagaaa ctcacccact tttctgcaaa
     7201 tgcaaaaaaa gacaatttcg atatcttgac tttgatctac gagttcgtcc aggaaaatta
     7261 tgagaaagaa atcaatttac aacttttatc cgaaaaatat catttttcat atagctatct
     7321 ctcagccatc tttactgaaa aatttggtat caacttttca aaatatctaa aaaaagtccg
     7381 tatcagtaaa gccaagctct ttttgacgga aacacggctt actttgacgg aaatctgtga
     7441 gaaagtcggc tataccgaac tgggttattt ctccagagtt tttaaagaag agacaggaat
     7501 gacaccttct caataccgta aaggaacttt tgtaaaatga gaaaaaaaat caaagagcat
     7561 gaactctttg ccaaacttct gttcattttg ttcatcggtc tttttctgca agccatcgtc
     7621 atttctttgt ttatttatca tcgatcaagg gatgcctata tccagctgtt caatcaatcg
     7681 aatgatgtgg tactaaaaaa gattcaatct gaatttgaaa gtttgaatga tacaatcgag
     7741 aatacattag ccgcttttga ttctaatcca gctgtcaaaa gctatttttc caatgatcca
     7801 gcccaacata tggaacagct tcagcaacta agaacaatcc aaaaaatgaa tgactctctt
     7861 tcaaaaatcc atccaatgat cgattacgat gtattgatct ttggagaaaa tggaagaact
     7921 tttgtcggaa acgacatgct cactgccgta tctgccgatt ctttttttca atctgcgatt
     7981 gctcagagag tgaatgaacg tgctgctgat acacaaatgc tttttgcaca ccacggattg
     8041 actcttcgcg ataagaaagc acccagcgtc ttctttgtca aaaaactgaa aaatacattg
     8101 aatcacgttt acggatatgc tgttttatcg atctcatcca agcaactagc gaatcttttt
     8161 caatctgtag taaatcctga gatatcgaag atctccttca tgaacgaaga acagacgatc
     8221 atcgcatcaa atgaagaaaa tacaattggg agccgatcaa tgattttcaa aaaactaaca
     8281 gtaggtgaaa caacgatttc aaatggacag cagttgaccc gtcttcctct gtaccgccag
     8341 gattgtacat tactcagtca agtcgatctt cattctttat ctaaccaaat gggcgtgatt
     8401 tttccgatta ttttgtataa tatcgtgaca cttgcttttg ctggctcatt ggtatttatg
     8461 tatttggatc gacacacaaa atcgatctac cgcttgattg attcgctaaa acggctagag
     8521 gaagatggct taaaagctac tgttcctgta caaggaagcc atgaagtcca attgctaggg
     8581 aatacgatca atcaattgct aagtaatgct cagcatcatt atgaacagac gatccaagac
     8641 gaaaagaaga aacgttcgct tgaaatccaa actatgcaag cacagataca gccccatttt
     8701 atttataata cgctgactag cttgaaattt ttgatttggc agaaaaaaac agatgaagca
     8761 gttcaaggaa tcgacagttt cattacttta ctaagaagta cgattgggaa aaaagaagaa
     8821 gtgattccag tcaaggaaga aatcaaaagc gtacaaagtt acatcgatct tttgagtatg
     8881 cgatatggcg aaagaatctc ggctaaaata atgattcctg atgaactgca gtcattatct
     8941 atgccgaata tgatccttca accaattatc gaaaacgcct atcttcatgc ttttcacaat
     9001 aaaacgcaag gattcatcac ggtgtacggc aaaacaaaaa atcagcttct tttattcgaa
     9061 gtgattgata acggcgatgg cttcgatact gaaaatacca aacaaaaaaa tgatcatttt
     9121 tcaggaatcg gcatcagcca tgtagatgag cggatacgcc taatgtatgg cgatgcatat
     9181 ggcttaacga tccaatcagt gattgggaca ggaacgactg taacgatttg tctaccgctt
     9241 cttcctaaaa atcaatcaga atatcaaaaa taaagaatag ccaagcgctg atttttttat
     9301 tttaagttaa ataaatcaat cagtattctt gactttcatc tacgcgtctc ttctactgtc
     9361 agcactggat aacagcccaa tatttgactt tctcctccga gcatcttgat ttcttggagc
     9421 gcattctcta ctaatgtcat tggacggtcc aacaacaaat cgatcacgaa aaaatattct
     9481 cccaagctag ttttcaatgg tcgggattca atcttactta agttgatttc ccgccagcca
     9541 aaagctgcca gcattttatg aagcatccct ggccgattag caggtagtgt caagatgaca
     9601 ctcattttta ctggagctga ctgctggctg gtcatctttc ggtcaccgac gatccaaaaa
     9661 cgtgtctggt tcaactcatt atcttggata ttctcagcta agatctccaa tccaacatgc
     9721 tgtgctgttt ctaatgaagc aatcgctgct gcgtcttctt tcggatgttc tgctacatac
     9781 attgctgcag cagttgttga ttctgttgct actaaaggaa cattaggata atgcgtttct
     9841 aaaaactgtt gtgattgtgc tagtgcttgt ggatgtgaaa ggatttttgt aatctttgtt
     9901 gctggattgc ctaaaagctg ctgcttgata ggaagaacga tttcactttt aacttcgact
     9961 tctggatgtt tggaaagaag atcgattgtg tgatgaacag ctccttccaa tgaattctct
    10021 acaggaacga cagccagatc gacttgtttg cgaaacaaag cgcgcaaaca aacagggatg
    10081 gaagcatacg cagtcaattg ttctgtagga aatagctgac aagccgcctg ataagtgaat
    10141 gaactttctg gccctagata gctgacgttc atatgctttt gatcctttct ataatggtat
    10201 cgatgatctt ttctggtgtt tgattggtcg tgttgattcg atgtgtcgca ctttcttcat
    10261 accaagaaag tcgttgtgcc aataatgctt ttatttcttc tcttgttttt ccaagagcta
    10321 aaggtcgaat agacgtttca tcttgttgga tacgatcaac tagacagtcc gcttctgcct
    10381 ctaagtaaag aacaatcggc tgtgttttta aaaaacgccg attttctgca ctttggacaa
    10441 tccctccacc agtcgcgata attttgtctt catttgacag ctttcttaaa atatctgact
    10501 ccacttttcg aaagcttttt tcaccgtaat agtgaaaata ttcggcaatc gacaattgga
    10561 tatgttcctc aatctttgta tctaaatcta catactcttt ttgtaaacat tgagctaacc
    10621 ctttaccgat tgttgttttt ccagcgccca tgaaaccaat cagcaaaatg ctagccatga
    10681 gcttctcctc ctgacacaag ttctgctaaa tcgtcaaaaa atgccggata agagattgaa
    10741 acagcttcag ctccttctag ttcgcaaggt ccatcagcta tcaaagcagc tacttgcagc
    10801 atcataccga ttcgatgatc gcctctgctg gacacttttg cgccatgcag aggtgtcggc
    10861 ccatggatga tcagaccgtc tgctgtcgct tttatctttg ctcccatttt ctgtaattct
    10921 tctgccaccg catcaattct gttggtttct tttactttta attcttctgc gtctttgatg
    10981 atcgtgattc cttctgcttg agtagccact aaagctagaa tgggcaattc atcaatcaga
    11041 cgcggaatga tttctccacc aatcgttgct ttcttcaaat gactcgtctt gacacttaaa
    11101 tcagccgatt ggttctgttc attgtgattc gtctgggtaa tcgctgcgcc catctcctcc
    11161 aacacatcta agataccagt tcttgttgga ttgaccccga cattttttaa caacagttgg
    11221 ctttccggaa caagaagtcc tgctgctaga aagaaagcag ctgatgaaat atcacctgga
    11281 acagttatct gttgaccagt taatttttgc ggtccggtca ctatgatcgt tttatcttcc
    11341 actgtgatcc ttccgccaaa ttgacgaatc atttcttccg tatggttacg ggaacgttct
    11401 ttttcaacga ttacggatgt gccttctgct tgtaatgccg caaacagcaa agcagatttg
    11461 acttgcgcac tggctacagg catattgtat cggataggtg ataaagagcg ggttcctgtc
    11521 acagtgattg gtagatactg gtcattttct tctccttgca agtcagcgcc catctcgcgt
    11581 aaaggttcca tcactcgccc cattggacgt ttagacaacg aagcatcccc gaacagagta
    11641 gtcgtaaaag gctgtccagc caaaataccc atcaataaac gtatggtcgt accggagttt
    11701 cccatgtcaa ttgattcggt tggttggaca aatgagtggc taccgcgacc atgaatcttg
    11761 atcacctctt cctcttcttc gatctcgaca cctaattgtt ggaaggcatg taaagtactc
    11821 agacaatctt ctgcgcgcaa aaaattttgt atagtcgtcg ttccttcagc aattgctcca
    11881 aacatgatgc tgcgatggga aattgattta tctgctggta tcctaacagt ccctcttaat
    11941 ccatgtattt gttgtaataa ttgcatttat ccagcctcct atttgatcca gcaatgataa
    12001 ctggtattag acataatgat tctttttgct tgtaacaaat cctgttggtt tttaaaactg
    12061 atttgcagta ttcctaaaat atcttccctg gtctcttgga tcttcaaatt aatcaaagaa
    12121 actcccgctt ttccgatcag tccagttact tctgcaataa cgccaggttc atctggcaca
    12181 tcgataaata aatcaaaaaa ggcaggaatc gcgccttgct tatggacagg caactggtct
    12241 cgactttctt ttgcccgatt gaaaaaagaa aagatttccg attggttctc agtcatgatc
    12301 cattctgcta tttgtgtcat ttcttgttgc caatctgcca atcgttccaa taatatctgc
    12361 ttattgctca acaaaatatc tgtccacatg acaggatctg aggaagcgat cctagtgata
    12421 tctcgaaagc ctcctgctgc cagttgtttg gctcgcggat actcttgatt gaattgttct
    12481 gcctgattga ctaagccagc tgccagaata tgtggaaaat gacttagcat tcctgttata
    12541 cggtcatgtt ctcttgctgt aagagttaca tattttgctt tggttccgca aaatagttct
    12601 tgcagttttt caacatcttt tttcgtcttt tccaaaggtg taaagatgta ataggcattt
    12661 tcaaatagtt tagcatctgc tgcctgtata cctgatttat gagaaccagc cattggatgt
    12721 ccaccgacaa accgaagatt tttctgctca gcggatgtca cgacttgctg cttcgtactg
    12781 ctgacatccg taatgatgac gtgatcagac aagttgattc tttcaaggac atcaagataa
    12841 gctaaggttg tacggacggg aacggctaat aagataacgt ctgcttgttc tgcacctgtt
    12901 tcaaaatcac ttgcaacgta atcaacgatc tgctgttcta ctgcttgctc acgggttttt
    12961 gactgactat cccaaccaac tactatcagt tctggattgg tctgacgaat acaaagtgcc
    13021 atagaagcac caatcagtcc aagccccacg ataaaaacat gttgtgccat cttatttgcc
    13081 ccctttttag taatgtttca agtagtcacg gtactccttc actgcagctt tcaactcaac
    13141 aagcgaatca cttgggaatt ttgacaatag ctctgtcgct accacagtgg ctacaacatt
    13201 ttccgcaacg acgctagctg caggaactgc tgtactatcc gaacgttcta cacttgcttt
    13261 gtaaggttct tttgtatcga tatcaacact ttgaagtggc ttgtacaatg taggaatggg
    13321 tttcatgacc ccgcgaatga caagtggttc gccattggtc attccgcctt cgaagccgcc
    13381 tagattattg gtacgtcgga aatagcctct ttcttcttcc cataggatct catccatcac
    13441 ttctgagcct ggtaatgaac cagaagtgaa tccgacccca aattctacgc ctttaaaggc
    13501 attgatacta accacagctt gtgcgatttt ggcatctagc ttcgtgtccc attgtacgta
    13561 gcttcctaat ccagctggaa cgcctccaac gagcacttct acaattcctc cgatcgtatc
    13621 tccatttttc tttgtctgat cgatcaaatc ttttattctt tgctcaactg ctggatcaac
    13681 aactcgtact tctgacgctt cagaaatttc tttgatttct tgaatcgaga tatttccagg
    13741 tatttctgct tggattccac caagacaaat cacgtgtcct gctacgatga tgtcaagttc
    13801 ttccagaatt ttcttggcaa ttgcgccgat tgctacacgc atggtcgtct ctcttgcaga
    13861 ggaccgttca agcacattcc gaagatctcg atgatggtat ttcataccgc ctaccaaatc
    13921 cgcgtgtcct ggacgcggac gtgcaactcg ccgcagtgat tggtcttttt caggcacttc
    13981 tgctacagac atgacagttg tccaattttt ccaatcttta ttttctacca ctaaagtgac
    14041 aggtgaacca attgttttgc cgtgacggat gccagacgtg atccgaacac gatctttttc
    14101 gatcttcatc cttcctcctc tgccgtaacc agcttgtctg cgagcgagtt cgtgattgat
    14161 ttcttcttct gacagaggta atcctgctgg tagtccttca ataatggctg ttaattctgg
    14221 tccatgggat tctcccgcag tcatataacg catcttacag tcctcctaag taatctttga
    14281 atgtctctgt gggaacagga acgatttttg cttgcccaat cttttctagc aagatgatat
    14341 tcaattggct tcctcttgct ttcttatcat gggttatcgc gtggaataat tcatctggat
    14401 gacttagatc gtacgtaatc ggcagatgga atttttgaag tatcctttct aaagcagctg
    14461 tcgatccagt tggagacaag ccgatttttt ctgcatgaga agtgatggct accattccaa
    14521 tagctactcc ttctccatga ctgactaccc catatcctgc tgtattttca attgcatggc
    14581 caattgtatg accaaaattt aaaataagtc gatttccttg gtcaaagaca tcttcttcca
    14641 ctacttttcg tttgatttcc aacgtagcag taatgattgg aacagcatgt tccagaagat
    14701 ctttttcgtc tttcagctcg ttcagctgtt tccaaagcgt tgcatctgct atagctgctg
    14761 atttgatgat ttcagcgatt ccttctcgta aacgacgtac cggcaaagtt tttagaacct
    14821 caggatcaat caatacacca gctggctggc taaatgttcc gacaaggttt ttcgctttgt
    14881 ttgtattgac tgctgtcttc ccgccaatac tagaatctac ttgtgcaagt agcgtagtcg
    14941 gaatttgaac gaaatggatc ccacgcatga acgtcgaggc cacgaatgct gctagatcac
    15001 ccaccattcc tccaccaagt gcaagtatcc catcgctgcg ggtaaaatca ttatctgcta
    15061 aaaaatcgta taactcagct gcttgttcta aagatttact ggtctcaccc gccggtacga
    15121 ttgaaaggac tggttcaaaa ttagcttgtc tcaattggaa catgattttt tctgcatata
    15181 gtggagcaac gtttgtatca gtgatcacag ctactttttg aggtctccaa atcgtttgaa
    15241 gccatgatcc agcctgttct agtgcgcctt tttcaatatg aatatcgtat ttttggttat
    15301 caacatgtac agtaagcatt cgtttcactt ctttctttta aaaagctgga attaatcgag
    15361 tgtctgaatc ttttccattg cttctttcat gatcgcaact tctttcatca tgcgtgagta
    15421 agtcttttca ttcaacgatt ggggaccgtc tgaccaagca tttgcaggat caggatggat
    15481 ctctacgatc atgccatctg ctccgcttgc cacacccgca cgagccattg gtggtacaag
    15541 ttcccaaatt cctgtaccat gacttggatc aacaatcaca gggaaatgag ttaatttttt
    15601 catcaatggg acagcactta aatcaaacgt attacgtgtc gctgtttcgt aagtccgaat
    15661 cccacgttcg ataaaaatga caggagattt gtcttcgact gcaatgtatt ctgcagcatt
    15721 aagccattca ttgatcgtac cggaaattcc acgtttcaat ccaacaggtt tacctgtttt
    15781 accgacagca gatagcagtt taaaattttg catattacgt gcgccaatct gtaggatatc
    15841 gctgtactca gccaccatat cgatatgccc ttcatccatc acttcagtaa tcactttcat
    15901 tccaaattca tcagccgctt gacggatgta tttcaatcct tcttcttcta aaccttggaa
    15961 agcatatgga gaagtacgtg gtttgaaggc accacctcgc agaatttttg cgcccccggc
    16021 tttggccatt cgcgcacatt ctctgatttg gtctaatcct tcgatagaac aaggaccagc
    16081 catcgtcacg aaactgccgt caccaatttt tactccgtct acatccacga ctgtattgcc
    16141 aggatggaat tctcgagaag tcaatttgta tgtgtttaaa atacgcacag cattttctac
    16201 tccttcatag cgtgtaaatg ctccttcttg taaacttcgc gtatcacctt ttagtccaat
    16261 cactactcgt tctgttcctt gatcgattga gactttgaat ccttcttttt cgatatgatc
    16321 gacgactttt tttatttgtt cttccgttgc ttcttttttc ataattacta tcattgtttt
    16381 tcccgctttc ttttgtttac ttatctatct tggctgattt tctctacttt ttcaaggtgg
    16441 ttttacatta attcctacta aatgaccgtg tcctgttgtt ctcattttcg ctccttcttt
    16501 cctacttatg acccagataa tcaacaaaaa aagatgtccc tgtaggaaat tgcctctaca
    16561 gtgaacatct tcaacgagcc ttaaaaagcg ctgcaaaaat ataaaatagc cactatagat
    16621 gaatttctct acgtggctgt aagtcgtgtt taaaaatctt tagccatcat agatacaaac
    16681 ctggtttgtt tgtacccacg aagtcatgaa tacaaaccta tctaaaatag ctaaagtaat
    16741 acgtattcag ttttgtaagt tttctagatt tcataaaaaa tcgctccaaa ctgatgtaag
    16801 aattgtacct agtatagaca taaaaaaatc gactgtcaat catttttatg aaactttttt
    16861 taattttctg atagttaact ttgtttttca gaaaaataac aattggtttt attgaagaaa
    16921 atagtcatga acaaaagtat ctgagaactt ttttactctc ttgttcatga catatagaat
    16981 aaagcagctt tgtcttgtaa actccgtttt tcaaataata atctaaagat tgctactgaa
    17041 taataaagcc gatttcatgg tcctcttcgc tgctgattcg gtaaggtgac tcaacatccg
    17101 ctccccagct attgataccc ccgactcctc ttacttttgc aaaaatcgta agaaccgttc
    17161 gtctttctgg cggtagttct tctatatgtg ttgcctgttc cagttcttct gctttgaatg
    17221 gtaaagcgga aaaagcaaat ggcgtatcct gctgttcaaa tcgcaacgaa aaatcttctt
    17281 gatctttatc cgtattatcc aacgtttgat ttcttgttag ttctacccat ttcgtttcca
    17341 tatgcatgcc gcattcttgg ggaacaaggt atggggaaac ggggagtcct gccactttat
    17401 aaataccttc gacaccacca gccatccgat ccggatatgt ttcccctgat aggccttcat
    17461 actcaaaaaa ttttgctttc gttggcataa tgaaccttaa acccaatgcg ggtaattccg
    17521 gtaatccttt ttgtccataa taatgcatat tgacaagcat tttaccctct ccagcgattt
    17581 ggtaggaaat tgtcacttgg gtttttggta ctgtgttgat ttccagtgta aaaatgattt
    17641 caaccgtttc agcgtattca ttacttgaat attgattatt tactggagca atcggcaaat
    17701 cgatcttttt attgtccacc agtacttcta tttctttgac ttttggatac aatccggctc
    17761 ctaaccacat accggattta aatccaaatc cgctacctct gtcattatcg gtcgttgctc
    17821 gccaaaaaac tggaagtgtt tcccggtata gccattcttt tccgttttta ttgagcgatt
    17881 ccagtccgcc tcttgtataa gaaaaaatat aatgcgtatt ccccgatttg acacctaatg
    17941 tacagtcgcc gaagacgatt gcaacttttt ccgtatccat atgactttta tttactgtat
    18001 ttttcataat aatatcttac ctcctgcatg gcgggttttt cttgtccatc tgcaaaaacg
    18061 atcccattcc ctgagaattc atagtcactt ggtttatcgt caaagtcacc gccatagcgc
    18121 atcacttctc tgcctgtgat tggatcagtc acttggatcg cctgatcttt aaaatcccat
    18181 atatagcctc cttggtacat ttcataacga tcaagcaacg tcatgtaaga atccattcca
    18241 cctaacgaat tccccatgtc atgcatatat tcacataaga tgaacggttt ttccgcttta
    18301 ttttcaaggt agtcaatgat ctcttctggt ggcgcataca tgcagctttt tacatctgaa
    18361 atcgaatctt cgaatgctgg atttggaaaa ttcccttcat aatggacaag tcgatcgggg
    18421 tctacttttt tgaagtaatc gttcatttgt cgaatgtttt cttccgcata agattcattt
    18481 ccaagagacc aaaaaagaat acttggatgg ttttttaatg tttcgaaatt cgtttttgct
    18541 cgatcaagaa cagccgcctg ccactgaggt actgcacctg gtacgttgta agaaggctca
    18601 aatgcgccca ttttttgcca tgagccatgg gattcgaggt tcgtttccgc catcacatgg
    18661 attcctgctt ggtcgcataa ataataccaa gtgatctggt tggggtaatg agatgtccgt
    18721 acgccgttga tctttgcatt ttttatgata gccaaatctt tttccatatc ttctatagtg
    18781 atggcgcgtc cttttttcgg atgccactca tgtcgattta ccccgttgat aatcatccgt
    18841 tttccattga agtagatgat tttatctttg atttcgattt tacgaaaacc gaaggaatag
    18901 tcaatatact ccgttactct tttttcttcg tctatcgtct ccaattcaag cttatataaa
    18961 ttgggctgaa aatgccccca cagtgccaca ttttcaaatc catcgatgcg aatcgtcatt
    19021 tgctctttta acggatattg ctgttgatag atttccttcc catcaggatc tagtatcgct
    19081 acattcagct gactgttttt ggctacttgc tcaaaagaca aatctacttc aagtttgcca
    19141 ttttgacatt tgtcatctaa gcgaggtttg atccaaagat cttctaaatg gttttgtcct
    19201 cgagcaacta agaagacatc tctgaagatc ccaaaaaatc ggaaaaagtc ctgatcttcc
    19261 aagaatgcag ctgtacttct tttgtaaacc tccacagcta gaatattttc ttcttccaaa
    19321 taaggtgtta aatcaaattc agacggagtg aacgaatctt cggcataccc gataaaatga
    19381 ccattcaacc aaataaacat tgcttgttct acaccagcaa aatgaatcga gatttgtttt
    19441 ccctttagac cttcttctaa tcgaaaagtc gtcaaatacg aaccaaccgg attgtcttta
    19501 gcagctgaaa agatcccttc cgcctctttc tcagggcttg tcgtgaaagg gggacggcga
    19561 aatacatgtc cctcccacgg atacatcgta ttgatgtaat ggatcttgtc ataatttgct
    19621 aattcaatat gactaggaac agtgatttca tcaaaagaac aggcatcaaa atctttttta
    19681 taaaatgttt ttggtcgtac cgaaggattt ggcgaataac aaaaacgcca tgatccattt
    19741 aaagactgtt taagggagga tgtggtttct acttcttccc ttgttgcata acaaacatga
    19801 tcgctatgtg catttgattg gttcactcta aaaacttgtg gatcatctaa ccattttata
    19861 tttgctttca tacttttttc cttctctctc ataacttttt gaaatgaagc agttctgttt
    19921 ggaaatcacc ttgaaaatcg gggaagtaaa gtccaactgt catcaattcg gcccccgaaa
    19981 atatccgctt cgaggtctgt tcctgataaa gtgcctcatc atctaacccc atcagatata
    20041 cttcatggaa cgctggttga gctgaagcta gtatcctccc taaaaacaca atagcttcat
    20101 cttgtttagg agacacaaac atccatgcgg caatattttc ttcaaaagga ctcgccaaac
    20161 gataataatg gccatactgg atcaccggac gaatcgtttg ataggatata atttggtctt
    20221 ttacttgctt ttcttcttca ggagacagct ttgtcaaatc cagttcataa ccaaaaacag
    20281 cgctcatagc tgtttctcct cttgttttca acgaagtaga tctgtcggtc tgatgatttg
    20341 gtacagctga tacatgagct gtgatggacg aaatcggata agcaaggctt gttccatatt
    20401 ggattttcat acgctccact gcatccgtgt tatcacttgt ccatgactgc ggcatataat
    20461 aaataaaccc ggcatcaaac cgtccacctc caccagaaca gccttcccat aagatttccg
    20521 gataatcagt tgtcaaatgt tcaagtagtt cataaagccc gagcatataa cgatgcgcta
    20581 cttctccttg tctctgtggg tcaagggtaa tcgaatagac atctgaaagc gaacggttca
    20641 tatcccattt gatgtagtca agaggaatcg tatctaaaat ctttctcatt tgtttctcaa
    20701 tcgtctgccg aacatctaac cttgtaaaat ccagtacatg ttgtgatctg gaagcagaag
    20761 gcattctacc tggctcctgc ataagaaaat caggatgtgt tcgatacagt tcagaatcaa
    20821 ccgagatcat ttcaggttca aaccataaac caaatttcaa tcctttgcta tgagcataat
    20881 ctgctatttc acgtaatcca tttgtcaact tgccttcata ctcgaaccag tctcctaatg
    20941 aagaattgtc cgcatctctt tttccaaacc agccatcatc taatacgaac atttcgatgc
    21001 ctagctcctt tgcttcatca atgatcgcct tgattttttc actattgaag tcaaagtaag
    21061 tggcttccca gttattgacc agaatcggcc gttcagcaaa ttgatgacga cctcttgcga
    21121 ttctttcacg tagcaactca tggtggatct gactcatttt gttcaatccc tgacttgagt
    21181 aagaaaggat catttcaggt gtttgaaaag actggcctgc attcaattcc cacgaaaaac
    21241 gatggctgtt gatcccaccg acgactctca attgattgat ctgatctttt tcaagttcga
    21301 aagaatggtt gccggaataa acaaattgaa gtccgatcgc ttctccagta ttttctgtcg
    21361 tatgatgatg aactaaagca ataaagttat tcatatggtg actgctcgtt cctcgacggc
    21421 tttcaaatac ttttgttcca gataaaacag attgccgtga aatctgtctt tcattgacat
    21481 gtgctcctgg caaagcaatg acttcatcaa atcgcctagt atgggcaaag tctagctgga
    21541 aactagccgc tttttctagt ttgattgttt ctccggtttc atttcttatc tttacagatc
    21601 gtgtgatcac tgctctatca gtaaatatgg tatagaacaa atcgaaataa atcgctgttt
    21661 ctctatcctt aagtgaaatc accaacgttt gtgcctctga cttgtccatc acataagttt
    21721 gaggcaagcc ttcgatctct ggcttcccat ctgtaatata atggctgtca tatctcagat
    21781 ccagtaagtc tgatccattc ttgcgctgaa tgatcgatgc gggcgttcga aagtccatgc
    21841 tgccatgaga agagtattct tgcggtagtg tatctttcga cagagaccgg tcaggattta
    21901 agggaacatt tcctgaaaat ccacgatctc ttcggggata atttttattg tctttgtact
    21961 gcttgactgg tttgccaaaa taaatatgcg ccaatacatt gctttcttct atctttatca
    22021 gataagaaag aaatgtattt cgtaaatgga agactcggct cttttcatca aataagatga
    22081 aatttttatc tgtttttcta tgcgtaatat ccatcatttc actcccaaca aaacggaaac
    22141 cgtttacttt tttttataat tcctgtatac tgtttactaa acaattttgt caataaaaat
    22201 tttttatttt tttgaggagt gtgtaaaaat ggttggaatt agagatgtcg cacgaaaggc
    22261 aaatgtctct cctgctacag tttcccgtgt gttgaataac gatcagacga tccatgtggc
    22321 tcagactact cggcaacgga tagtagaggc agctgctgcg ttggattatg acatcagtac
    22381 tcgtaaatat atcaaaaaga gaatgccttc gattggtgtg atctctacga tcggcaaagc
    22441 gagcgaagaa caggatatct attacaaaca tttgcgagta gggatggaag aagaagctcg
    22501 acgtctgcat ttagggatga atcgaatcta taatctatct gataatccta aacaatggga
    22561 agatctagat cagttaggtg cggtgatcgt tgttgggact gttacgaaag aatcgattac
    22621 cgccttgtta aaacaaaaca tgcaaatagt cgtcgtggat aaccctgaca tccagcaaga
    22681 ggtcgatatg gtgtatgcag atcttgaacg gatgactaaa accgtactgg aattgttctt
    22741 agaaaatggt cataaaagaa tagcctatat cgggggatat aaagtcgata tggatgaatt
    22801 cggtgggaag cagatttcta taaatgaaaa gaggatgcgt gcctatcagc attttatgat
    22861 cgatcatcac ttagaaaaat atatgtctta tcatctaggt ggctggacag aaaaacaagg
    22921 tgaggtactt gcgactaatc tattagaaaa tgaatcccct cttcctactg ccattcttgt
    22981 cgggagcgat ccaatggcaa ttggtgtcta tcgggcattg aaacaaaaaa atatccaaat
    23041 tggagaagag attgttgtag caagctttga taatatcgag acaatcgcaa acctctcccc
    23101 tggcttgacc agtgttcaaa tcaatgcgaa atcaattggg caagcagctg ttcgaatggc
    23161 attggagcga attgacggta tacgggacga accaatcatt ttgacttatc ctgcacgatt
    23221 gattgttagg gaaagtttta tcccaagaac aaaaaggtat tgaaagagag gttgtgaaaa
    23281 gccgcttcgt ttcttcatac tcaaaacgaa acggcttttt cacaacctct tacttatact
    23341 tattcacaaa atccaatgtc tgctggtaac aattttgcga ttcttttagc gtgtaatcaa
    23401 attggtattc atgggtaatc tctttttttg tatctgtata aaacaaggat tgcacgggta
    23461 cctttaattc tgtcaaccga ttttctaatg cttgaccttg ctcctggaat gagtatgcat
    23521 ttccgtcagt tatatacgtt ggcggaaatt ccttagaaac atggtcagcc acactagctt
    23581 ctttcaattg ctcactcgtt ttccaatctt ttgttccaat caacgaccac gcaacggttt
    23641 tgacgaaaaa cttcataaat cgatcatccg aatgctgctg tgctgttagc ttcaaatcaa
    23701 caggtccgca ataagaaatg gttcctttca gactatcttt tggaagtagc tgtttgattt
    23761 tcatttgttt agcatacgca tcattcgttt gtatagctgc atattgcaat gcgatttgtg
    23821 aacctgcact atctccacca aaaaataatt gttccatgtc tagcaatttg tcgtcttttt
    23881 cttcagctaa taaatctttg atcagttcat tcacttgaag aacttgatta gggtattctg
    23941 aatcaggcgc taattcataa ttcatcgcaa ccactgcgat gttggcgtct gaaactaatt
    24001 ttgtcgcaaa ttctttgaca cccgatttat ctccgccgac gaatcctcca ccatgtaccc
    24061 aaaatagaac aggaacaggc cccttgctgt tttttggata gtagatgtca taagtattct
    24121 ctttatactt ggactcatac gtcaaatctg tttttttcgc cacgtttttt tctgcttgat
    24181 cgaacgtttt tttatcagtg atttgaacag tatttgaaaa catatggttg atcaagaatg
    24241 ctccaggtct gggggaaagc tggaaggcaa gaaagattgc aaacgcgatc ccaagaatga
    24301 ttccgaagaa ccatccgata aatttaagaa ttttcttttt cataaggctc cctcagtagt
    24361 tagtattaag cattctgatt ataccatagt gttatttcct atcaagctct cctagatgat
    24421 ttaatgattt tccccaaact tcttctgcaa tgcttttgac aaaagaaatt ttttccattg
    24481 ttcttcaaac aaaacatttt cttcttctgt tgaagcaaat ccacattgag gacttaagca
    24541 caaacgatcc aaaggcagat atcgtgtcgc ttcgtagatc cggtcaatca cttgctggtg
    24601 ctcttccatt tcgccatttt tcgttacagt caaaatctaa gacaactaat ttatcttcag
    24661 agactttctc taaaagaaca aaactcccag cacgttcact atcgtattct aaataatagg
    24721 cacggacatt ttcttgagta aaataatggg tctgctacgt cgtcatcccc cctgatgccg
    24781 ccaaaatcga ataataattt ccgcgtcaaa catgtatatt tagtgttgaa tcatttgaga
    24841 ata
//
LOCUS       DAJSKS010000028        24338 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJSKS010000028 DAJSKS010000000
VERSION     DAJSKS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.029"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..840)
                     /locus_tag="ON396_000984"
     CDS             complement(<1..840)
                     /locus_tag="ON396_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3113086.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMD"
     gene            complement(1064..3004)
                     /gene="thrS"
                     /locus_tag="ON396_000985"
     CDS             complement(1064..3004)
                     /gene="thrS"
                     /locus_tag="ON396_000985"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR3113087.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3341..3520)
                     /locus_tag="ON396_000986"
     CDS             complement(3341..3520)
                     /locus_tag="ON396_000986"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCR3113088.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3567..5390)
                     /gene="cadA"
                     /locus_tag="ON396_000987"
     CDS             complement(3567..5390)
                     /gene="cadA"
                     /locus_tag="ON396_000987"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCR3113089.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5668..7947
                     /locus_tag="ON396_000988"
     CDS             5668..7947
                     /locus_tag="ON396_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3113090.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8040..8687)
                     /locus_tag="ON396_000989"
     CDS             complement(8040..8687)
                     /locus_tag="ON396_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113091.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8866..9585)
                     /locus_tag="ON396_000990"
     CDS             complement(8866..9585)
                     /locus_tag="ON396_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3113092.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9716..10867)
                     /locus_tag="ON396_000991"
     CDS             complement(9716..10867)
                     /locus_tag="ON396_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCR3113093.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11084..11824)
                     /locus_tag="ON396_000992"
     CDS             complement(11084..11824)
                     /locus_tag="ON396_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3113094.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11828..12151)
                     /gene="rsfS"
                     /locus_tag="ON396_000993"
     CDS             complement(11828..12151)
                     /gene="rsfS"
                     /locus_tag="ON396_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR3113095.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12208..12801)
                     /gene="yqeK"
                     /locus_tag="ON396_000994"
     CDS             complement(12208..12801)
                     /gene="yqeK"
                     /locus_tag="ON396_000994"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCR3113096.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12785..13435)
                     /locus_tag="ON396_000995"
     CDS             complement(12785..13435)
                     /locus_tag="ON396_000995"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR3113097.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13445..13756)
                     /gene="yhbY"
                     /locus_tag="ON396_000996"
     CDS             complement(13445..13756)
                     /gene="yhbY"
                     /locus_tag="ON396_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR3113098.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13772..14881)
                     /gene="yqeH"
                     /locus_tag="ON396_000997"
     CDS             complement(13772..14881)
                     /gene="yqeH"
                     /locus_tag="ON396_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCR3113099.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14878..15405)
                     /locus_tag="ON396_000998"
     CDS             complement(14878..15405)
                     /locus_tag="ON396_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCR3113100.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15585..16370)
                     /locus_tag="ON396_000999"
     CDS             complement(15585..16370)
                     /locus_tag="ON396_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR3113101.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16363..17232)
                     /locus_tag="ON396_001000"
     CDS             complement(16363..17232)
                     /locus_tag="ON396_001000"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCR3113102.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17233..18612)
                     /locus_tag="ON396_001001"
     CDS             complement(17233..18612)
                     /locus_tag="ON396_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR3113103.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18620..19039)
                     /gene="fabZ"
                     /locus_tag="ON396_001002"
     CDS             complement(18620..19039)
                     /gene="fabZ"
                     /locus_tag="ON396_001002"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR3113104.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19064..19540)
                     /gene="accB"
                     /locus_tag="ON396_001003"
     CDS             complement(19064..19540)
                     /gene="accB"
                     /locus_tag="ON396_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR3113105.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19543..20778)
                     /gene="fabF"
                     /locus_tag="ON396_001004"
     CDS             complement(19543..20778)
                     /gene="fabF"
                     /locus_tag="ON396_001004"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR3113106.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20797..21534)
                     /gene="fabG"
                     /locus_tag="ON396_001005"
     CDS             complement(20797..21534)
                     /gene="fabG"
                     /locus_tag="ON396_001005"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCR3113107.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21546..22463)
                     /gene="fabD"
                     /locus_tag="ON396_001006"
     CDS             complement(21546..22463)
                     /gene="fabD"
                     /locus_tag="ON396_001006"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR3113108.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22570..22794)
                     /locus_tag="ON396_001007"
     CDS             complement(22570..22794)
                     /locus_tag="ON396_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR3113109.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22847..23809)
                     /locus_tag="ON396_001008"
     CDS             complement(22847..23809)
                     /locus_tag="ON396_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCR3113110.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23819..24259)
                     /locus_tag="ON396_001009"
     CDS             complement(23819..24259)
                     /locus_tag="ON396_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3113111.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6653 a         5427 c         4242 g         8016 t
ORIGIN      
        1 atccataaaa tattcttgga ttgcttctcg tgtttttttc actttttcgc taagcttttc
       61 gtttttagcc atatagaaat aaaaaatgat atcgatcgta gcaaattggg ccaaaagtga
      121 attagttgcc gcacttcgat tcgtgatttc ttttggtcta gatgtttgta cgagtacatc
      181 ggctatcttg ctaagcgggt tatttccaaa ttgcgtcaac gcaatgattt cgatattcat
      241 gcttttcgcc agctcggcta atgcgactac atctgcactt ctacctgagt ttgaaacaag
      301 ccaaagcact ttctttttct cattgctgac aagttgcggg agcaagacgt ggatatcttt
      361 ttcaaaaatg attggcttac cgacacgaga ccatttttgc agaatatctt ctactactaa
      421 agaagaggcg ccaaccccat ataaaaaaac agtctctgcc gattccaaac actctacgac
      481 tttttggatc gtttcttctt ctagaatatc tactgtttct tttatcgcta cttgtgcatt
      541 gttaccaagt ttgtttttga tagagtaaaa ggactcattt gcttctatat cactaaaacc
      601 ttcatcgatt ccagtctgta gttctgttga aatcgtgatt ttgaagtccg tcagactttg
      661 aaaaccgatt tttttggaaa aacgtacaac tgtcggcgca ctcaacccgg aagcttctgc
      721 taattcatta gctgtcattg tagggacctt ctcaatatta ccgataatgt agttggccaa
      781 ctttttttct gctgaagaat aatcttccat catactttca atacgactaa ttacgctcat
      841 aagttcctcc acccttttaa tgatagtcta tgtaagtata ccatatcaga cccttattca
      901 cggtcgtata ctggataaaa aacaaaaaaa caatccgccc aacagaatat cttatcccat
      961 caatcccaca gattctattg agcatctgtg tctttgagga atacctgtta tctattgttc
     1021 ggattgcttt ctatctacta cttttttaca ggaaatttac ctattagttt tctctgctgt
     1081 aatttttgac ttctgcttcc atagcatcaa taaagatatt caaatcttct acagctgttt
     1141 ctttacttcc ataacgacgt acgttcactg ttgcatcttc caattccttg tctccaacaa
     1201 ctaattggta agggatcttt tgtgtttgag aggcacgtat cttatagccc attttttcat
     1261 tacgatcatc aacttctaca cgcaacccct tcatttgcaa acgttctttg atttcataag
     1321 cataatcgcc atgtgcatca acagaaacag ggatgattgt tgcttgtata ggtgccaacc
     1381 atgttggaaa agcgcctttg tatacttctg ttaaataagc gacaaaacgt tccattgtag
     1441 aaacaattcc gcggtggata actactggac gatgcgtatt ttccccgtct tctccaacat
     1501 atgtcaaatc aaagcgctct ggcaataaga agtctaattg tatagttgat aatgtttctt
     1561 ctgtaccaag agctgttttg acttgaacat ctagttttgg tccgtaaaag gctgcctcgc
     1621 cttctgcttc aaagtaatct agttctaatt catccatagc ggctttcagc attgcttggg
     1681 ctttttccca cattgcgtca tcatcaaaat atttatctgt attgtttgga tcgcgatagc
     1741 tcaagcggaa acgataatct gtaatattga agtcagcata tactgccacc atcaattcaa
     1801 gcgtacgttt aaattcatct ttgatttgat caggacgaac aaatgtatga ccatcattca
     1861 acgtcatttc tcgtacacgt tgtaaacctg acaaagcacc tgatttttca tagcgatgca
     1921 tcattcctaa ttcagcgata cgaattggta attcacgata agaatggatc gtgtttttgt
     1981 agaccatcat gtgatgaggg cagttcattg ggcgaagaac aagcatttcg ccatctccca
     2041 tatccattgg cgggaacatg tcttcttgat aatgatccca gtgtccagat gttttgtaca
     2101 actcaacatc tcccattatt ggtgtatata catgttggta acctagacta acttctttgt
     2161 ctacgatata acgttcgatc gtgcgtcgga ttgttgcccc ttttggaagc cagaatggca
     2221 aaccagaacc tacttctggg gaaaccatga acaagtcaag ttcttttcct agtttacgat
     2281 ggtctctttc ttttgcttct tcacggagac ggatatattc tttcaaagct ttcttgtcaa
     2341 aaaaggctgt cccgtataca cgttgcatca tttgattgtt tgagtttcca cgccaataag
     2401 cacctgccac tgataataat ttaaatactt gtatacggcc agtggatgga acatgtggtc
     2461 cacgacacaa atcgatgaat tcgccttgtt gataagcagt aatcacttct tcttctggca
     2521 attcactaat cagttctact ttgtacggat cacttgcaaa aatttctaat gcttcctgtt
     2581 ttgaaagaac acggcgttcg atcggcagat tttctttgac gattttcatc atttctgctt
     2641 cgattgccgg caaatcttct gcagttactt ggttttgtcc attatctgta tcgtagtaaa
     2701 accctgaatc gatagctggt ccaacgccaa agtggatatt tggatagagg cgacgcatgg
     2761 cttgggccat caaatgagct gctgaatgac ggactaagcc aagtgcatct tcatggtctg
     2821 gtgtgatgat ctcaatactt gcatcttcct cgattggacg agtcaaatca acgagttttc
     2881 cgttcacttt accagctaaa gctttttttg ctaagctttt tgaaatgctt tcagcaattg
     2941 ccaatgttgt tgttccagac tcaaattctt tgactgcacc atctggaaaa gtaattttta
     3001 tcataatagt ttcctccttg aaatgttttg attgatcaga tcatcaagct gaaaaacaaa
     3061 aaaatcctag catctcttga aaagagatac taggacgaaa agttcgtggt tccacctaat
     3121 gttcaaacgc aaatgcgttc ctcaagcgtt gtaacggtcc gaccgccctt gcttcaacaa
     3181 gggtttcgaa agtggtcatc ctttggtttt ctcctggaaa agtttcagcc tagcttttcc
     3241 tctcttatcg aagactctca aattcagttg gctttctcat caatcaatta tgtgtttatt
     3301 taaccactaa taaatagaaa aatcaagtgg gaaactaaac ttattttttc cattccccat
     3361 ttacaccgta tgttcctttt ttcatctcat tgatgatgac atggatgtgt tcttttggtg
     3421 cctgcgtgtt tttatgaaca gcttccgtga tatctttcat catattttct aattgttccg
     3481 gactgcgacc ttctactaat tcgacatgta cgaatggcat aatgctctct cctctttctg
     3541 atcgttcttt tttcaactcg attctatcat actatgtttg tcttgggtac tgaatcagga
     3601 aaaatggctg gatctttttt tgctctcaag gcactcaaaa tagagaccgt atccacgact
     3661 tcttgcaaaa cagctccgaa taatgcgggg attagtcccg tacttgcaat taacatcaaa
     3721 aaaatacaaa taaagatgcc aatcagtaca gactgtttcg ctattctcat cgtatctctt
     3781 gaaatcacta ctgcttttgc tacacgcaag agatcatctt tcaagatgac cgcatctgca
     3841 ctttcactag cagctgtaga accatgagcc cccattgcaa taccgatatc tgcagcagca
     3901 agtgctggtg cgtcattcac gccgtctcct accatcgcaa caggtctttg atctttaggg
     3961 atactttcta aaatggtcag tttatcttga ggcaagctac gggcatgtac agaatctaat
     4021 tggacttttt ctgcaatcgt ttgagcaatg gcagatccat ccccggtgat catcatcaaa
     4081 tttttcacat caagttttct tagctccttg attgtctttg ctgcttctgg gcgaatctga
     4141 tccgaaaata ggatcgttcc tgcaaaagat cgatcaattg ttacatagat tgctgtattg
     4201 gacgtttcta tttcttcagc tgaaacaaaa gaggcacgtc caatctttac ttcatgatta
     4261 ttgactactg cttggattcc ttgaccggtc acttctttca gttgactaac aggatacagt
     4321 tggctgtttt tcacgttgtt cactaacgaa cgagctaaga tatggctaga ttcctgttct
     4381 gcactagctg catacagaag tagctcttct ttttttattc cattgactgg ctgaatcgcc
     4441 tctacttcca atatcccttt ggtaagcgtt cctgttttat caaaagcaat cgtttttgta
     4501 cgtgcgagct tttcaatcgt tgttccagtt tttacaacga tcccgttttt acttgaacgg
     4561 ctcattcctg caactaaagc aatgggcgca gctagaatca gtggacaagg tgatgccact
     4621 accagtacct cagcaaatcg taaagggtct tttgagaacc accaagaaat accaccgatc
     4681 aaataagcaa tcaacgtgaa agggactgca taacgatcag ctaaacggac aaatcgggca
     4741 ggcttttctt ttgattcttc gactagttta ataatcattt gatactggct gtctgcggca
     4801 cgtttttcag cgatcatttg gatcgatccg tctccattta aagaccctga cataatctga
     4861 tctccaggtt tcttggtttc cgggcgggac tcacctgtca aagaagattc atcgaccgaa
     4921 gattctcctt tggtcactcg accatctgcg ggtacaagtt cgccaggctt gactagtaac
     4981 tggtcatcaa tatttatttc atcgattact atatcctgca attttccttc tttgaatcga
     5041 tgggcaattc ttggggtgtt atctaacaaa gactgcagtt ctttacccgc tcgtttattc
     5101 gcgtaatcct ctagactatc tccgcccgta agcatgatca atatcatcaa actagcccag
     5161 tattctttga cggctaatgt tgcaaggatt gcggtaatcg ctaatacatc tacgccatat
     5221 cttcctgaac gtaacgtctt gatcatttcc aaaaacatca agaaggcgag aatccctcca
     5281 gtaaatgcga caatcgcaaa tgcaagggcc ggctggtgaa aaacaaattc acttaataaa
     5341 gctatcccac caataagcaa agtaagtaaa aattttttga aatgtgtcat tgttttttct
     5401 cctcttttat gtgacttaga gaggttagta ataaatttcc agctgcctct ttcatcttaa
     5461 gcatagtcct tgactagcac tttttaaaac aataggcctt aattgaaaac aactatcact
     5521 atcagcgttt ccaaaattca tcagttctac aaacaaaaat attcagcaag gattactttt
     5581 tcgattcatc agttaaaaag tacctttttt cgaaaaacta tgctatactt ttaattcgtt
     5641 atttacggcg gaaggagcaa acatgcgatg aatgagaaac aactagaaca aacgcacgtg
     5701 gatgaaacga tcgaactaat ccagatcgaa caaaatatgc tggatacaaa acaacaagaa
     5761 ttgactcagg aaatgaatca ttcgacaaaa gacacagcta atcacaagat tcgagctggt
     5821 tccaatgaag ctttttatga atctgctgtt gaatatcgcc aacatgagca ggaattgctt
     5881 ttgaaatacc atactcttga agcacagcaa aaacgcctgc aaacgttgtc tgtcatgaaa
     5941 gatagtcctt attttgcccg tatcgacttt aaagaagacc gtgaaacaga aacattatat
     6001 ctaggtatcg cttctttaag agatctggca gaagaaacaa tcgttattga ctggcgcgca
     6061 ccaattgcca atctttatta tgaaggtgag attggtcccg cattttatga aactgaaaca
     6121 gatagaatag atgtcgatct gcagttgaaa cgacaattta aaatccaaga aggacgtatt
     6181 ttatctatgg tagatacgtc ggaagtaatc aatgatgact ttttgctaga gatattggat
     6241 gaatcatcaa gcaaccatat gaaaaacatc gtctctacga tacaaaaagc acaaaatgca
     6301 atcattcggg acacaacaag taaagtaatg ctgattgaag gaatcgccgg aagtggaaag
     6361 acatccgctt tgcttcaacg tattgctttt ctgttgtatc ataatcgcaa gtggctcgag
     6421 gctgaaaacg ttcttctatt ttcacccaac catttgtttt ctgattacat ctcgactgta
     6481 ttgccttcat taggtgaaag cggcgtgcct acacagacat tcaaaaatta tttggtgcag
     6541 ctgctgccag agttttcttt actagatgaa cagcaacaag aaagtggttt tttgctaggg
     6601 gaaaaagatc cgatacaagt gatgaaaagc ggattgacct tagtcgatca gattgatcgc
     6661 tatatccaag gcatcacaaa ctacggtccc ttgttccgtg atatgaaaat aaatggccgt
     6721 acgatcctat caaaggaatc gattcgccaa tggtataaag aaacaaatga acaattacct
     6781 ttacatcata gactttctct attacaaacg aagctcctga aaaaattagg cggtctgcaa
     6841 aaagacgaaa cacgtcagca atgggtcaaa gatctggcgg aagaacagct ccaagaacta
     6901 tacgcaacag atccaaatct tgaatataca gaacaaaagg agcgttcatt acgtaaaaaa
     6961 ctggccaacc aaatcgtacg taaaagattc cgtcgaatcc atcgagggat catgaattat
     7021 caatttgtca atataacgaa acaatatctc cactttctac aaactgtgcc actttctctt
     7081 ctaacacgac ataaaatcag cgagaccagg tggcaggaac atttgacaac tgttcgtaca
     7141 cacttgcgtc aaagagaatt gcgtcaagag gatgctgtac tattcttttt actgatgcgc
     7201 cgaacatatc cagtgacggt tacacacaaa gctcgctata tctttattga tgaaatgcag
     7261 gatttcgcac catcacaagt ggcattgctg caagccttgt tcccaaaagc aaatttgact
     7321 ttctgtggag atctaaatca aaatgtcttt ggaaatgaaa cgatcgttgg atcattggaa
     7381 gaccttttcg tggaccgtga agtcactcgt ttccaattaa cgacaagcta tcgctccaca
     7441 aaagaaatca ctgattttgc taatcatttt attgtgaaag aacatcaagt agaaacaaca
     7501 gcaagaaaag gagaccttcc tttgctcatc caaggaacaa cagatgatat caaattgaat
     7561 tggttagcag ataccattcg aacgactcag ggaaaagccc ggtattggcg tacagcaatc
     7621 atagctaaaa cacaagaaga atgcgaagct ctttatgctc aactgccaga cgagttgaaa
     7681 caacaagtcc agctgatttt aaatgagagt gactttatga agcgttccat tattttactt
     7741 ccagcctttt tagcaaaagg tctggagttt gaccgagttt tcttgtggga tgtagatgca
     7801 gaacattttt cttctcagca agacaaattg atcctttaca ccatgtgtac acgtgcgatg
     7861 catgaattga ccctttttac ctccaataat gaaagtcctt tgttaaacaa tatggatatt
     7921 tcatcttatg agaaaaaagt actgtaagta aaaagacaac tgctatcatt aatgttagca
     7981 gttgtctttt tatttacaat aataattgca gaaaaagtaa ttcaatcatt taaggtcatt
     8041 tattctatat ataaatccaa atctgtttca ggatatttta atttgacaag cgaaataatc
     8101 attttcagtt cttccatgtt ttctggatat tctgggtcaa ataccttgct atgacggcca
     8161 atcccgccat tgtcgatcaa tcggtaaatt cgattccatt caatgaattg gtcaattgat
     8221 tggaaattgc tgattggata gccgccacaa tccaaaatct ctgatacacg aaatgctagg
     8281 tatgcactat cgatcacttt attttttttc gtgtctatct tctcatttct ttcgataaga
     8341 attttttcag gacgatgttt caagctccac tctaatagta gaaagaaagc ctccatccga
     8401 tactcttctt caaataaccg aaaggctgaa tcagaacgac gaaaaagcag cggaatcaga
     8461 taattttgtc tataaatcag cattggtgcg cggtccacca tacactctaa ataacggttc
     8521 agataccctg taccgacata gtttcctaat cgatagcctt caacgccgaa tcttgcgtat
     8581 ttttgcgcta attttgaaca atcagttacg ttcacagcta actcttctac tttgcggtaa
     8641 ccgcttaaga tacgttcaaa aggttctaaa gtgatttttg cttccatatt cttccccgtc
     8701 catcttccac atacttcata tcatttcaca tgatacttat gctctcatca taccacaggt
     8761 tgaattagaa aagaaaataa atcacacaaa aaagcaagac aatctgttag tgagattgtc
     8821 ttgcttttcg aaaaagactg aatacttttc gttaaaactg gttgcttagt cttctaatgc
     8881 cacattatgg aacacatgct gtacatcttc tagatcttcc agtgcatcaa tcattttttc
     8941 aaatgtttct tgatcttctg gagatagtgt tacctcgttt tgagggatca tttggatctc
     9001 agcgattgaa aaatcagtga ttcctttttc ttttaatgct tcttgtatac tatggaaatc
     9061 cgctacttca ccgtaaacga tgatttgtcc ttcttcttcc attacatcgc gaacatcgat
     9121 gtctttttcc attaggtatt caaggatctc gtcagcgtct tctcctgcaa aagcaaagat
     9181 tgctgtattg tcaaacatgt aagaaaccgc tccggaaaca cccatgttcc cgccattttt
     9241 cccaaatgct gcccgtacgt ctgctgctgt tcgattcaca ttgttcgtca atgtatcaac
     9301 aatcaccatt gagccgcttg gtccaaatcc ttcatagcgc aattcagagt atgtttcatc
     9361 tcctgcacct tttgcttttt cgattgctcg gtcaataata tgttttggta cattataagt
     9421 tttagcgcga tcaatgacga aacgtagttt ttggtttgca tgcggatcag gatcacctga
     9481 ttttgctgcc gcgtagattt caatcccaaa ttttgcataa atccggctat tattagcatc
     9541 tttagcagtt ttctttgcta cgatatttgc ccatttacgt cccatatttt cacttcgctt
     9601 tctgatttga tagtttcata acgcaaaaac gtcctgttcc attatacttg tgaatcacga
     9661 ttctgtgaag ctttttttct ttcttccttc attcttctca tttttagtca attatctata
     9721 ctcggattgg gatttgcccg aaattctgtt ccggaatatt gcctcccaat tgatagattt
     9781 gatcacttct aactaacagt tccgcttctt gtgcctcttt ttttccgatt cgggaataaa
     9841 tagaaatctc ttttttttct tttaagaatc ttcttccagc atcggtaaat ccaagtagtc
     9901 gaagataatt tttattttgt tggacaggaa tctcttgctt tttgatattc agcaagacat
     9961 aacaagataa tcgttggatc cgtgtccatg tatatctttt tgtttttacc tttgacacaa
    10021 aatcctcaaa actatcagca gttgcagctg ctgttttcat gcgcgattct attccttcgg
    10081 tcatctgata gatatctgtc agttccagca aagtagaaga taacaaccga taacgcaaat
    10141 acggccaata agattcccaa gtctgcagcg aacaagttga aagatcttct tctgtctgag
    10201 gcggcacaag tccagctatt ctttttcctt cttctaacgc atggcgaatg gctgttgcac
    10261 tggctatcct ctgatctgtg atgatttttt catgatagcc tgcactttta cgtcggatcg
    10321 gatgtaattc catagggtgg acatacttgg cattttcctt tgcataactt aatcccaaga
    10381 tatgatttgg cgaagaaaaa tcaagcgtaa tatcgggata cagactgcga aaaacctcag
    10441 tcattttttg cggataacta tacgtttgat cagacagttc ttgaaatgcc tcttctatcc
    10501 tatcttgatt ctcttggaca aagcgtccga aactgctgta atcaaacgaa gtctcttctt
    10561 ctgttccaaa acacaaactg tcacagtcca gtgtttgcaa gatttttact gcacctttag
    10621 caaaatagtc agctgactga accgcccatt cgacgggaag ctccactact aaatccacac
    10681 cattttgtaa agcttcattc gcgcgatgcc atttatcgat gatagccggt tcgcctcttt
    10741 gcaaaaaatt cccgctcatt acagcaatga cgacctctgc tcctgttatt tttcgtgcca
    10801 tttccacatg atagcgatgt ccattgtgaa aaggattgta ctctacgata atcccgcagc
    10861 ttttcatgcg catcctcctt catagttatc gataaaaaag aaatagagac aagtaaaaaa
    10921 aggcaaaaat cggaaaaaat acccgaacta tataaactat tctcgcaaac acaatacttc
    10981 tgcgacgaaa agctgcactg caagagcaat gcctatatcg ttcggatata taatttatgt
    11041 tgaagattta ttcttttctt gttaactcga gcaggcaaaa ccgctagtct ttgtatgcta
    11101 caaagaacca gcgctttgat ttctctgatg ctgcttcgtc tgtaaaatca gaaaatgctt
    11161 cgactttcga aaagccgctg ttttccagca ttcttaaata actttccaat ggataggtac
    11221 gctcttgatg tagttcatct tcacgaataa atcgaccaga cccttcttgt tcttcaacga
    11281 aaaaggtaag aaaatgttcg atgctgtgtt tctcgcttcc tggataacta tcccacaaaa
    11341 aagcaaaatt atccccttga taatgatagc tatattctgg aaaaacctca tctacttgat
    11401 aggtggagtg tacgtcaaat aaaaatcggc cattttcttc taacgcttga tagacttcat
    11461 caaacacttg ctgtacctct tgtctatttt tcatatagca cagcgaatca gaaaaacagg
    11521 taattgcctg atattgtccg atttctgata aatcgagcat attgccttcc acaaattgga
    11581 tcgatgcgtc ttcttcatat gctcgctggc tggcaatcat caacatctct tctgaaaggt
    11641 ctaaccctgt cactttatat ccatcttttg caaaggcaac cgctaatgct ccagtaccgc
    11701 atgccagttc caaaatattt tttgtgtctt cttgtaaatg acgtttcgaa aaagctaacc
    11761 attgctgata aagtgtttcg tccatcactt catcgtaaat gaaagcaaat gtttcataag
    11821 ccatatgtta atccaaccat tcactaagat cgactagcgg agcatctgac catagttttt
    11881 ccagattata gaaatttcgt tctggcgcat ggaaaacatg tacgatcaca tctccaagat
    11941 cgatcaatac ccattttccg ccttcttttc cttcaatacg ttttacttca tatttgtttt
    12001 cttcttcttt atcaatgatt tcttctgtaa ttgcgttgat ttgacgttcg ctatttgctg
    12061 aacagatcat gaaataatct gccaacaagg aaacatttcg aacgtctaaa gcgataatgt
    12121 cttctgcccg ttttgaatct gctgcttcaa tcgcaatttt taattgttct ttactatcta
    12181 tgatgatttc ctcctaatat gagcagctta tttttttgct acccagtaat tatatgtttc
    12241 gattgttttt ggataaatct gttgttcttg cgcaagcaaa tgggccaatg tatgttttgt
    12301 ttcaaatgct acagcttcgt ctagatcagc ccatgcaatg gctcgtgcat cttgtacccc
    12361 aggaaaatct cgacctggtt caatatagtc tgctacataa atgattttgt caaggagact
    12421 catcttagca gccccagtcg tatgcacccg gattgcatga agaatctctg catcggtgat
    12481 tcctaattcc cgctctacaa acgaagcgcc aacaagacca tgccagattg cattattata
    12541 attcaataac tccggatcaa aaccgtctct tacgattacc attttgaact cctcatctgg
    12601 tcgttctttt gcataatcat gtgtcaatgc agcaatacta gctttttcag gagaagcacc
    12661 gtattttttg gcaagagcca cggctgtttc ttctactcca agcacatgct tgaagcgttg
    12721 ttcgctcatt ctcatctgta ttttttgcat caggttttct ctagaataag cagtatattc
    12781 attactatag tccatattca tacaaccctt cattttgaat ataatcaatt actttatctg
    12841 gaacaaggta gcggatagaa cagccttctt tgatcttttg acgtattttc gtggaactga
    12901 tgtcgatctc aggtacatct acccaaatta caggatatgg agtatccgtt gtgtatcctg
    12961 gtcgacgtat acccacaaag ttgaccatag aagtcagctc atcgatttta taccattttg
    13021 gcaaatattc taccatatcc ccaccgataa taaagtaata atctgtatcc ggattgtttt
    13081 gcgtcaattc cttcatagta tcataggtgt aacttttccc accacgagct aattcaatcg
    13141 tttcaatctg caaaaaagga ttgtcttcca ccgctaattc caacatgttg agtcgatgtt
    13201 ttgcatcaat cgtctgtttc tcatcaacat gaggtggctg ataagaaggc atcaaaaata
    13261 cgcggtcaag acctaaattc cggccagctt gttcagccat cactaaatgt gcaaggtgaa
    13321 cgggattgaa tgttccgccc aagatcccta cttgcttttt cttctctaag aattgaggca
    13381 tttcttcagg aaataattgt gaacgaacaa atgtcttagc ttgcgtattc atcatggtca
    13441 ctccttatat tgcttttact tcttttgata gcctttgata cttttctttt gtggaaggtt
    13501 tgtataatac aataacacgt ccaatgattt ggacgatttc gcaatctatt tctttttcta
    13561 atacttgagc tgcttcttct gcaatctcat ctgtattttg aagtaacgat actttgatca
    13621 gttctctttt ttctaaagct tcttcaattt gaacgatcat tgctgaattg atcccacctt
    13681 ttccgatttg aaaaatcggc tgaagatgat gcgctttgct acgtaaaaaa cgcttttgtt
    13741 ttcctcttaa actcataatt ttccttcttc cttaaatcaa tgctttgcgt ttcaatacat
    13801 ctacaccctt tggcgcccag cccgcaatta ctactggcgc tgttacagtg atccagccta
    13861 gaccggcaaa aacgatgtct gttttttctt tcacagaaaa ctcaaaacga acaagctccg
    13921 gaaatgcctc gacttcatca ggacgaggag gctgtaataa tccacctaca tgtttttgat
    13981 aaaattcgtc tgctttttcc agtttcgttc gatgaatatt tacatcattt gacacataag
    14041 caataaatga agcacgattg ccgctgataa aatcaaatct tgcaagtccg cccaagaaaa
    14101 gtgtttgccc ttcattcagc tgataaactt taggtttgat ttcttttaac ggtgcaatga
    14161 tccgcaaatc ttttttacct aagtagtgcg ccatttgatg gcgatggata attcccggtg
    14221 tatcaatcaa aaaatgcccg tcatctaaag gaatctcgat tttatccaag gtcgttcctg
    14281 gaaactgcga agttgtgatt acatcttgta ctcccgctgt atttttgatg atttgattga
    14341 taagcgtcga ttttccgaca tttgttactc cgacgatata gacatcccgt ccatcgcggt
    14401 attcttcgat tttttctaat agttccgtca tctcatgaga tttcttagca ctagttaata
    14461 atacatctac tggacgaatt cctgcttcgt aagcacgttc cttcatccat tgtgttaatt
    14521 tagttttttt caatgattta ggtaatatat ccactttgtt cccaactaaa agtactggat
    14581 tgtcaccgac aaaacggtgt agtcctggga ttagtgaacc attgaagtca aaaatatcca
    14641 ccacattgac gatcaatgca tctttttgcc ctaattcatt cagtaagcgc aaaaagtcgt
    14701 catccgtcaa tgagacatct tgggtctcat tataatggcg taatctgaaa caacgctgac
    14761 aatagacttc cccggaagct aaaccttttt ccaaagccgt ttgcggcgtg tatcctaatt
    14821 caccgggttt gtttgtttgt ataacggcac cacagccgat acattgtaat tcttctgtca
    14881 ttcgattcca cctcgccagc ccatatcagg gtgttttgct aatagatagg ccataattct
    14941 tttttcccaa aaacgattaa attgtgtctt ccaactatct gttgcgataa tgggttttac
    15001 aaggatgcta cgtatccctg cacgattggc tccacgtata tcagtcataa tctgatcgcc
    15061 taccattact atctcatcat ctgctaaatc caattgtttc tttgctatgt tgatccctct
    15121 tgctaaaggt ttcattgctc gtgcaacata caaaagatca aatttttcaa tcgctcgcgc
    15181 gacacgtttt gaattattgt ttgaaacaac aacaacaggg attcctgcat ttttcatttc
    15241 taaaatccaa gtaagcaact cttctgttcc atctggatta ttccatgcaa tcaacgtatt
    15301 gtctaaatcg gtcaatactg ctttgatgcc tagattcttt aattgggcag gtgtaatctg
    15361 atagatggct tctaccatcc atgttggttt gtaatttgaa aacatgctgt ctcctcatct
    15421 gcaaaaaatg aaggagcaca taaagtactc ctcaaccccg tattatactg catatcagca
    15481 aaaaaaacta cctctatact cgttcgtctt tcagaaaaag catttcttta caaaatgacc
    15541 aaaaataaag attgatcaca gaaaaattta gaaacaaaaa acaactaata ttggcggaac
    15601 ctcttgtatc gtttttccag taatttatgc tgatctaatc gaacaagttc cgtcaatctt
    15661 tcaatcaacg cttttttcat aatgtgtgcg actttatatc gtggcaactg ctcgccgttg
    15721 aacgtttcag gaataactcg atcaacgatt cctaactcgc gtagttctgg cgcagtaatt
    15781 ttcatcaact cagctgcttc tgcggcgcga ctaccgtctt tccacaaaat cgacgcaaat
    15841 ccttctggag aaagcacagc ataaatcgtt tcttccagca tccagacttc atccgctaaa
    15901 cctagagcaa gggcaccgcc acttccacct tcaccaatga tgatggcgat gatcggtacc
    15961 tttaaatcag acatttcgag cagatttctg gcaatagcct ctccttcgcc gcgctcttct
    16021 gctcctacac cgcaatatgc acctgctgta ttgatcaagg taatcaccgg tctattgaac
    16081 ttttccgctt gtttcatcag tcgtaaagct tttcgatagc cttcaggatg gggagaacca
    16141 aaatttctgc ggatattttc ctgcagatcc cgtccttttt gaataccgat cactgttacc
    16201 ggtttgccat ctaatcgagc gattccacca acaatcgctt cgtcatctgc aaataaacga
    16261 tcaccatggc attcctcgaa agaatcgaag atctcttcaa aaaaatccaa gcttgtccat
    16321 cgttctttag ctcttgctaa ctgaacgaca tcatgtgctg tcttatccac gatctatcca
    16381 tcctttctgt gtatgcaatt caagaagcgt atggattttc tgtttcaatt ccatgcgggg
    16441 aacaatctga tcgacaaaac catgagataa taagaattca gccttttgga aatcttctgg
    16501 aagttcctgc ttgatcgtct gttcgatgac gcgtcttcca gcaaaaccga tcaagctttg
    16561 gggttctgct agaataatat cgccttccat tgcaaaactg gcagttactc ctccggtcgt
    16621 cgggtccgtt aaaacagtta gataaaaaag accttcattg ctatgccgtt tgacagcagc
    16681 agagattttt gccatctgca ttagagaaaa gattccttct tgcatccgtg cgccacctga
    16741 agctgtgaac aagacgactg gaagactttc ctttgttgct cgttcgaata atctggtgat
    16801 cttttctccg acaatcgttc ccatacttcc catgataaaa ttaggatcca tcactccaat
    16861 aatgaaggga atcccctgaa tggttgcttt accagtgagg actgcttcat gaagtcctgt
    16921 tttatcttgc atttttttaa ttttttccgt atattcggga aatgctagcg gatctttggt
    16981 tacaagatcc gtatcccatt cttcaaaact tttttcatca ataatcaaag ctagacgctg
    17041 ccatgcaccg attcgaaaag cgtatccgca atgagggcat actttttctt caccgatgtc
    17101 tttcgtataa aggatatgtt tgcaatttgg acactttgcc cacatgttat caggaacaga
    17161 tggcgagtta gcaggtttgc tctctctatt aggattgatc cgaatatatt tctttttctt
    17221 aaacagagcc atctatgcgc ctcctatctc cgtttctggt gtccaattcg gtaaaaattc
    17281 ttcttgtaaa aatgctgtgc tgtaatcacc tgctatgact gcaggatgac taatcaaatc
    17341 catctgaaat tctgcgttcg tgatgattcc ttctgtgata aattcactta aggctcgctg
    17401 catcttcatt aatgcttcaa aacgcgtatt tccatggacg attatttttg caatcattga
    17461 atcataatat ggcgggatcg tatatcccgg gtaaactgca ctgtctactc gcagtcccat
    17521 cccacctgct ggaagcagta agttttggat cttccctgga gaaggagcaa aatggaaagc
    17581 aggattttct gcattaatcc ggcattcgat tgcgtgtcct tggaatacta cttcttcttg
    17641 acacacattc aaaggctcac cggctgctat ctccacctgt ttttttacta aatcgattcc
    17701 tgtgaccatt tctgttactg gatgttccac ttggattcga gtattcattt ccatgaaata
    17761 aaaatcgcct tcttcatcca tcagaaactc gatcgttccg gcattttcat aattgactgc
    17821 tttagctgct cgaacagcag cgtctcctag tgcttctctt tttgtttgtg agatcactac
    17881 agaaggagat tcttccagta ctttttgatt gttgcgttgt aaagaacaat ctcgctcgcc
    17941 taaatgaatg acatgaccaa atcgatcacc taagatctga acttctatat ggcgagcagg
    18001 ataaatgatt ttttccaaat acatatcatc atttccaaat gccgctgctg cttcttgctg
    18061 agctgaagaa aaatgctgtg ggagctcttc ttttgattga actttccgaa tgcctttccc
    18121 gccaccacct gcagctgctt tcagcatcac tggataacca attctgtctg ctatttccaa
    18181 tgcttcttct actgtagata atacaccagt acttcctgga atgactggga catttgcttc
    18241 ttgcatcaat tgacgcgcat tgatcttatt tcccattgca tcgatcgttg cacttttagg
    18301 accaataaat gtgatattgc actcttcaca catggaggca aatcggctat tttcagataa
    18361 aaaaccaaat cctggatgaa ttgcttcggc ttttgtcacg attgcagcgc tcagtacttc
    18421 ctgaacattc agatatgaat caacggcttt tgccgggcca atgcagattg cttcatctgc
    18481 taactgtgta tgaagtgctt cggcatctgc ttctgaatat atcgctacgg tttggatccc
    18541 tagttcgcgg catgcacgaa tgatacgtac agcgatttca ccgcggttag ctatcaatac
    18601 tttagaaaac atatttcacc tatccaatca taaaggttaa ttccgcttcg gctacttttt
    18661 taccgtctac tttagcgatt cctttgccta ttccggctga agctttcact ttcaatatct
    18721 caacttcaag gtataaggta tctcccggtg taactttctt acggaatttt gctttgtcca
    18781 atccgccgaa ataagccgtc tttcccttga attgttcaag agaaagtaat gcaactgctc
    18841 ctgcttgagc cattgcttca acaatcaata cccctggcat aacaggttct tctggaaaat
    18901 gtccttgaaa aaagggctca ttgatcgtta catttttctt agcaacgatc cgttctcctt
    18961 caaccatttc ttccactcga tcaatcaaaa gcattggata acgatgtgga atgatttctt
    19021 ttatttcttg tatgttcatt gttttccctc tttcctgctt tgcttatttt tgctgaatcc
    19081 taaagagtgg ctgtccatat tcgacaactg actcgttttc tacaaggatt tctgtgatca
    19141 ctccgtcggc ctcactagtt atctcattca tcagtttcat cgcttcaata atgcagataa
    19201 cttctccttt ggcaacttga tcacctactt ttttgaaagc tggtttgtcg ggcgctggtt
    19261 gcagataaac gattcccacg attggcgaaa caacctcgat tccatcaaat actgccgatt
    19321 ctttttcttt cattatttct ggagcagttg ttgcttgttc aggtatgaca ttgactggct
    19381 ccgaaactgg caacgtttcg tttgtttgaa ccatattcat gcctcgactt gttttatttt
    19441 tattcatata caattcaaat tgaccttcac gcaaatcgaa ttccgttagc gaggattgat
    19501 caaattgact gactaattct ctgatttcat tgatatccaa aattattcct cccagcgttt
    19561 catgcacaga accgcattat gtccgccaaa ccctaatgaa ttactcattg cataagtaaa
    19621 acttgcaggt ttgctttcgt ttttgacaac tgtcaagtca atctcaggat cttgtttttc
    19681 tacattgatc gtaggtggga taaattgatg ttctaatgcc agcaaagttg ccaaggcttc
    19741 gatcccgccg gctgctccca ataaatgtcc agtcatcgat ttcgtgctgc tgacgtaagt
    19801 atctctgaaa ttttcaccta atgcgatttg gattgctttt gcttctgcct ggtcattggc
    19861 tggtgtactt gttccgtgag cattgatata tccgacttgt tccggctgta caccagcttc
    19921 ttccatcgca agcttcattg ctttagatgc accagaacca tctggagtag gagctgtcat
    19981 atggtaagcg tcacagtttg caccgtatcc aacgatttct ccatagattt tggcaccgcg
    20041 agcttgtgca tgttctaaag attcaagaac tagaacgcct gctccttcac ccatcacaaa
    20101 accattccga ttttcgtcaa acggaatcga accacgatta ggatcagttg attctgagag
    20161 tgctgttaaa gctgcaaatc cagcaatccc catctcacaa atcgttgctt ctgttcctcc
    20221 agcaagaatg acatcagaat agccatgttt gatgttacgg aatgcttccc caattgaatt
    20281 cgttccgcta gcacaagctg ttacgatagc tgtacaaata ccttttgcac ctacacgcaa
    20341 agcaatattt ccagctgcca tgttggcaat cgccattggt acgaacattg gagaaactcg
    20401 ttttgcacct ttttcatgta gacgaataac ttggttttcg attgttggaa gaccaccgat
    20461 cccagatccg atgatgacgc caaaccggtc ttgattcatt ttttctttat ctaaaccgct
    20521 catctccatt gcttctaaag cagcgtagat tccatagatc gaaaacatat ccattcgttt
    20581 gctatctttt ttaataaaat atttatcgta agggaaatct ttgacttcac ccgctaaggt
    20641 aatccctgtt tctgaagcat caaatttagt gatcggtgcg atgccattcg tcccctcttt
    20701 taagctattc aaaaagctgt ctgcgtcatt tccaataggt gaagtgactc catagccagt
    20761 aattaccact cgattcattt gtttgctctc cttttcttat ccgtgcatga ccaatccgcc
    20821 atcgacattg atgacttgtc cggtgatata tggattttgt gctaagaaaa ctgctgtttg
    20881 tgcaatatct tctacttgtc cgaaacgctg catcggaatt tgcttttccg cttgttcctt
    20941 gactttgtct gctaaaactt ccgtcatatc agttgtgatg aatcctggcg caatggcatt
    21001 acatgtaatg cctctagttg cagcttctcg tgccacagat tttgttaatc ctactacgcc
    21061 agctttacta gctgcataat ttgcttgacc aatatttcca atcaacccag atacactaga
    21121 gagattaatg atggcccctt ctcgctgctt catcattttt ttcaatacgt gctgcgtcat
    21181 attgaaggtt ccaatcaaat tgatatccag acattttttg aaatcttctg catccattcg
    21241 catcacaagt ttgtcatttg tgattccggc attgtttact aacacatgga tcgatccaag
    21301 cttttcttct gcttctttga tcatttgtcc cgctttctca taatcagaga tgtcaccgga
    21361 aactcctacg cattttacac cgaatgcttc gatttcttct atcttttctt ttggaatctc
    21421 tccgcgacca ttcaaaatga tatttgctcc agcttttgca aaagctaagg ccatcgcttt
    21481 gccgattcct cttgtactac ctgtgataaa tacatttttt ccttttacat ccataacgtt
    21541 cctccttata gtattgctat tgtttcatca aatgtctgct tattctcaac acgcagaact
    21601 ggtattgttt tatcgatttt tttcataaat ccgcttaata cttttccggg gccgacttcg
    21661 atgacttgtt cgatgccgat tgtttttaac ttatggatac tctcatagaa acgaacaggt
    21721 tgcatgactt gctgttcaag tagtcccgcg attgtttctt tcttcattat ttccgttgtt
    21781 gtgttgctta tgataggaaa agaaggttca gagaattgaa tctgacttag gtccttcgcc
    21841 agtttttttg ctgccggttc taaaatagct gtatgaaaag gcccgctgac atttagaggg
    21901 atcattcttt tgaacccttt ttcttttaat agtaaaacag cttcgtctac tgctttttct
    21961 tcgccaccaa tcacgatctg ctgaggtgta ttataatttg caggagatac gatgccgtac
    22021 ttgctagctt catgacagct ttcctcgatc gtttcaatcg gcgcattcat cactgcaacc
    22081 attttcccgc ttccagctgg ggcagcttct gtcatataag cgcctctttt tgctactaac
    22141 gcaactgctt cagtgaagga tagcgcatta ctagctacta atgccgaata ttctcctaaa
    22201 ctcagtccta aagctgcatc tgggatgatt ccatgctcct tcaataagcg ataatacgcg
    22261 atgctgactg ttagaatagc tggctgtgta tattgcgtct gatccaaacg ttcattttct
    22321 gtaaagcaaa gttcagccat atcgtagccg agaatctcac ttgcttcatc aaatgtctgc
    22381 tttacaacgg cttcttcata taaatctttt cccatacctt gatattgtgc accttggcca
    22441 ctaaataaga atgctgtttt catgtttttc tccaatatat ccagaatata tgtttgattc
    22501 ttcctattca aaaaggccga gacattccta tttcaggaaa tgtcccagcc cccaacctaa
    22561 ttttaatgat tatgcagttt gtttttctac gtaagtcact aaatcttgaa ccgttttgat
    22621 tccgtcttct gtttcgattt tcacgtcaaa ttcatcttca atttcattga tgatttggaa
    22681 taaatccaag ctgtcagctt ctaagtcttc ttggatgtca gtagtcaatt tgatttcttc
    22741 agtttctttt cctaattctt ctgcgatgat ttcttgaatt ttttcgaata ccataataat
    22801 atattcctcc gatattgttt gctttttatt tttataattt aacagtctac aactccatga
    22861 gaatacttcc ccatgttaaa cctccgccat agcctgtaaa tattactttc tgctgttttc
    22921 ctaaggtgat ctttccacta gaaactgctt catccaataa gatagggatg cttgcagcag
    22981 atgtattccc atatttatcc atattggtca ggaacttttc ttgcggcatc tttacttttc
    23041 gggcaatttt ctcaataatt ctgaggtttg cctgatgcaa aagcagataa tcaactggtt
    23101 tgtctttcat gacttcttgg atattgtttg caacgtctcg tacggcaaaa tcaaaaatat
    23161 ctctgccagt catttgcaaa taatacgaac cttctgaatc ttctgaatag aacggatttt
    23221 tattttgctg gtagccagcg gtcaatgagc tacttctggt accgtcagct gctaattttt
    23281 ctttcagcaa ttttttctgt ggattcgctt ctaagagaat cccacctgct ccatcgccaa
    23341 acaacacagc agtcgtacga tcagaccaat cgattagctt agatagtgtt tcaccaccaa
    23401 tcaccagtcc cttagttgag ccgcagcgga tcattttttc agccatgctt aacgcataaa
    23461 caaatcctga gcacgcagca ctgatatcaa aagcgatagc atgagttgct cccaatttcc
    23521 cttgtacttg tgctgctaca gatggtgtcg tgtaatcggg cgacattgtt gctactaaaa
    23581 taaaatccaa atctttaggg tttagatttt tctgttttat caatttttcc gccactaaaa
    23641 tgcataaatc agaagtctcc tgatttgtta cacatcttcg ttcacgaata cccgtccttg
    23701 acgaaatcca ttcatcagag gtatccatca tcttggaaag atcattatta gtaataatct
    23761 tttcaggaag gacgctggct gttgcagtaa tcattccaaa ttgttccatt aaatatcctc
    23821 acttatagtc ttgcaagaag tcgtgcaagt ttttcagtgc atccagcaag gcatgctgtt
    23881 cctccgtagc cattccgtct aagatgttct tgaccatttc acgatgaaaa tgctgatgga
    23941 cccgaaatag taactttcct tttttcgtta gtcccaactt tacaacgcgg cggtcatctt
    24001 cactgcggat acgttcgaca tagccttttt ttactaactt gttaatagca gtggtcaagg
    24061 tcccaacagt aattgatagt tctttggcta cttctgaagt tgtttttttc ttgtacatcc
    24121 cgattgcctc gatcgtgtgc atctctgtga ttgacagatc tttgaattga gattttttca
    24181 gctcagactc ttcgattgtc aaaatatcat tgaagacact aacaagataa tcatttactg
    24241 tttttaaatc tggttccatg ttccaccgtc cactaattag tttgattatc aaatcttttg
    24301 atgttcaaac tatatcttaa cttttttcaa aatgcaac
//
LOCUS       DAJSKS010000029        24337 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJSKS010000029 DAJSKS010000000
VERSION     DAJSKS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.030"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(133..2478)
                     /locus_tag="ON396_001010"
     CDS             complement(133..2478)
                     /locus_tag="ON396_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCR3113112.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2757..3659
                     /locus_tag="ON396_001011"
     CDS             2757..3659
                     /locus_tag="ON396_001011"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR3113113.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3870..5645
                     /locus_tag="ON396_001012"
     CDS             3870..5645
                     /locus_tag="ON396_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HCR3113114.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5711..7297)
                     /locus_tag="ON396_001013"
     CDS             complement(5711..7297)
                     /locus_tag="ON396_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR3113115.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7548..7772
                     /locus_tag="ON396_001014"
     CDS             7548..7772
                     /locus_tag="ON396_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCR3113116.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7914..9665
                     /locus_tag="ON396_001015"
     CDS             7914..9665
                     /locus_tag="ON396_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3113117.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9665..11449
                     /locus_tag="ON396_001016"
     CDS             9665..11449
                     /locus_tag="ON396_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3113118.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11784..12636
                     /locus_tag="ON396_001017"
                     /pseudo
     CDS             11784..12636
                     /locus_tag="ON396_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            12733..14619
                     /locus_tag="ON396_001018"
     CDS             12733..14619
                     /locus_tag="ON396_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3113119.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14660..15562)
                     /locus_tag="ON396_001019"
     CDS             complement(14660..15562)
                     /locus_tag="ON396_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3113120.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15792..17195
                     /locus_tag="ON396_001020"
     CDS             15792..17195
                     /locus_tag="ON396_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR3113121.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17192..18628
                     /locus_tag="ON396_001021"
     CDS             17192..18628
                     /locus_tag="ON396_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR3113122.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18773..19699
                     /gene="trxB"
                     /locus_tag="ON396_001022"
     CDS             18773..19699
                     /gene="trxB"
                     /locus_tag="ON396_001022"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR3113123.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19912..21639
                     /locus_tag="ON396_001023"
     CDS             19912..21639
                     /locus_tag="ON396_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCR3113124.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21655..22005
                     /locus_tag="ON396_001024"
     CDS             21655..22005
                     /locus_tag="ON396_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR3113125.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22099..22959)
                     /locus_tag="ON396_001025"
     CDS             complement(22099..22959)
                     /locus_tag="ON396_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCR3113126.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23051..23479
                     /locus_tag="ON396_001026"
     CDS             23051..23479
                     /locus_tag="ON396_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR3113127.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23641..23817
                     /locus_tag="ON396_001027"
     CDS             23641..23817
                     /locus_tag="ON396_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR3113128.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23844..24290
                     /locus_tag="ON396_001028"
     CDS             23844..24290
                     /locus_tag="ON396_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HCR3113129.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7708 a         4352 c         5187 g         7090 t
ORIGIN      
        1 ggtttttcac atttttacat aaatttgtct taatatcgta gatgtaagct tttgaacacc
       61 atttattcgg aaaaagggag ctgtgacaag atttttgtcg cagctccctt taattctgat
      121 taatctatta gatcaatcat ctttatcgtc tgattctttg gtctctggat tcgctctagc
      181 ataagctgaa gcggtgttcc agtaatctgt atagttttca tcagtaccgc ctacgccgaa
      241 tttaaaggcg cttttcttcg gaccattttt tgcccaaagt gactccactg tttttcctgg
      301 cgtttggatg gattttttat tgacagtaac ttttccagct gtttgtcctg tgaacccaga
      361 tacttttttc ttcatcacat cggatgataa gtcaaatttc tcatcagtgc cgaaaatctc
      421 agggtttgct tggtaagctt gattgattag attggcaaga taattagccg aacgtattct
      481 tgcttggctg tccattccag tattgtcatc gtgtcctgcc caactactga tagtgataga
      541 tggagttgag acgatcagcc atgaatctcg gtattcattg gtagaaccag tttttccgac
      601 ccaatctgca tttcctagat ttccgtttaa gcttgagatg acatctttga atggtgtagt
      661 gattttttca ttgatcacac tacgcatcat gtcattcatg atcgatgccg ttgcttcgga
      721 ataaatacgg acagaattac tttcatgttt ataaatcaca ttacctgcat tatctgtgat
      781 ggaatcaatg atatagcctt cttcgtagac tccaccattt gctaaggcct ggaatccatt
      841 tgtctgttga agcgtcgtta catctactgt ccctaaagga gccgattcaa cgccccaatt
      901 atcatttgct gggtagttca tcttcgccaa gtagtgctcg taagcgtatt gtttcgatcc
      961 accggaattc aacacatctt gatataagtg ataagctggg atattgttgg accattccag
     1021 tgactcacgg acggtttgga aggtgttcga cccttcattg gtcgcattga cgattttctc
     1081 accagcattt tttccttctt gccaagtcgt tgcataatct gatacacggg attctgatcc
     1141 gacaagtccc atatcgattg caggaccgta aacaagaacc ggtttgatgg atgaaccagc
     1201 ttgacgttct gtatcaaagg cgtggttatt ttgattctgg ctgtaattcc gaccacccac
     1261 gaagccatag atacgtccgg ttctgttatc catcaataca tttccagtct ccacttggct
     1321 accagcacca tcatctaaca tgtagccata attttctacg ccggtttgta atgcagcata
     1381 gatatcttta tcgatcgtcg aatggatcgt atatcctttg ttttggatcg tttgctgtgc
     1441 tttttgataa taagcttcac tgaccgatga atctgacata tcagcattat ctttttctgc
     1501 caattgttgt gcaataatcc ttgttgcttc gttcatcacg gtataataca agaattctct
     1561 gtcagtctgt tccgcgattt gttgtgggag aaagtctttt gtaagatcat aggctttcgc
     1621 ttcttcgtat tgttctttcg taatttgtcc ttcacggtac atactgaata aaacaaaatc
     1681 ttttctggca agaccggcag aaagatcttc ttttagcgcg cctgtattcg tgtatggact
     1741 atacgtgata gggctttgag gcaaaccagc gatataagca gcttgtggca gagtcacatc
     1801 ctttgcatct acaccaaaga ttcctctagc agcttcctgg actccagcaa tattttgccc
     1861 cttattattc cgaccaaatg gtgagacatt caaatacgta gtaatgatct cattttttga
     1921 aaagtgtttt tctacttgtg cagaaagaac gatctcgttt gctttacgtt tgaatgttgt
     1981 ttcatcggtt aggatctgtt gtttgactaa ttgctgtgtc aaagtggatc ctccagacga
     2041 gccgatgcca gtcgcttctg aaactagtgc tcgtaaaaca gctttaggga cataaccttg
     2101 atgtttatcg aagtattcat cttctgtact gataatcgcg gttttcaata aaggcgaaat
     2161 cttatcagat gaaatagtcg tccgcatcag atctgtctgg atttttgata tctctgtatt
     2221 atctgcataa acaatctttg acgtttccgt aatgtttcct agttctgtct gcagttcttt
     2281 tttagtcggc aattgtgtgt cttcgacaag ataggcaaag tagccagcac ctattccgat
     2341 acctagagct gcgaacaaca aaagaagaac agtaataaaa acagtcaatg attgaaacac
     2401 tctaaaaata atcttaggaa caaaccattt ttcttttttt cttctattct gtttttttcg
     2461 agtatttttg cgtggcaaaa gggtcacctc aactaatggt tttatttcgc taattatagc
     2521 aaataatagg acaaaaaaac agacaaggct gtttttgaca ggaatttctt tttcgaatga
     2581 gataaaacgt gaggaaatga tcttttttat gaaagagttc ttaagctttt ctttagggaa
     2641 agtttcagtt actatggaga acagggcaaa gaaaaacaaa ccattttctt tgtacatcat
     2701 cttatcaata tacaggaatt gttcgaaaca attgcctttc aaggaggaat ccatccatgt
     2761 tattaggctc acatgtcagc atgagcggaa agaaaatgct gttaggcgcg gcagaagaag
     2821 cagccagtta cggatcttcg acctttatga tctatacagg cgctcctcaa aatactagaa
     2881 gaaaagctat cgaagagatg aatattgatg ccggaaaagc atttatggca gaaaacggac
     2941 taacaaatat cgtcgttcat gcgccttata tcattaattt gggaaatacg atcaaaccag
     3001 aaatgtttcc atttgctgta caatttctga gagaagagat tgtccgtgcg gaagctttag
     3061 gtgctactca aatcaccctt cacccaggcg ctcatgtagg agcaggggca gaagctggga
     3121 ttgctagaat tattgagggg ttgaatgaag ttttgacaaa agaacagacg gcacagatcg
     3181 ctttggagac gatggcaggg aaaggtacgg aaatcggtcg aacatttgag gaattagcgc
     3241 aaatcatcga tggagtaaaa ttgaatgaaa aattatccgt tacttttgat acatgtcata
     3301 cgaatgatgc cggatataat ataaaagaag atttttcagg tgtaatggaa gagtttgata
     3361 aggtcatcgg attagatcgg cttaaagtac tgcatatcaa tgattctaaa aatccacaag
     3421 gttctcataa agatcggcat gccaatatcg gttttggaac aattggattt gacacattga
     3481 acaaaatcgt ccatgatcca atgtttgaca acatttcgaa aatattagaa accccatatg
     3541 tcggtaaaga taaaaagaaa gcttacgcac catatggaaa agaaatttcc atgttcaaag
     3601 cgcaagcatt cgatcccgaa gtgttccctg agttattagc agaagaagtc acactttaag
     3661 ttttctacct gacagcaaca aaaaaggatt gggcagtctg ccttgtcctt ttttgtttaa
     3721 gaaacaaagg agtgcttttt gcattcgttt tcgccatgct ccttttgccc cctcttttta
     3781 aattcagaca aacagggtat actgatagaa aagtaaaaaa cgaaaagttt cacaaacaag
     3841 tagcaaaacg tgattgaaag gacgaaaaga tgctgaacat tttaaacccc ttgatcgacc
     3901 aactaaaaaa gaaacataca tactttattc ttggcatagc cgtattattt acaattattc
     3961 ggttgtattt atatagtaaa gcaatctatg gaacagatat gtcggcgacc tatgatgatc
     4021 agctgctgat tcactatgct caaaatattt tggatggtaa atggctaggg gagtatacga
     4081 ctacaacact ttccaaagga atctcctaca gcttattttt agtacttgca cataaactgc
     4141 atctttctta tgcagttttg ttcggcatgt tcagtatttt tgccagtata ttggttgcac
     4201 tcgcgtttaa accgattgtg aagaacaagt atcttctttt aagtatctat ttttattttt
     4261 tgttttctcc gattagtttt acacatgaat attccactcg aacatatcgt aatgccctcg
     4321 tgattccggc tgttgagatc attatcgctt gtttattagc tatttattac cgcaaatacg
     4381 gttctagcag aaaattgctg ccatggtttt tagggctttc tgtcgctttt cctttctttt
     4441 ggttcattcg cgaagattct atctggcttc ttccttttac tgtagtagct ttgggtatca
     4501 gtttcgtcca gatcttgcta gggcaagact tttctttcaa agaagtaaag aagttaaaaa
     4561 aacgactctt tcttctctcg aaacgggaat ggctgaaact ttcattgttt ttacttccta
     4621 tagttggctt ttccatcgtc tatcagtgga tcgaatataa aaatgagaca acatacggta
     4681 ttgcaaccat caatgaccgt tccagtggag aatttggacg tttgatgaaa caactgatcc
     4741 gaatagatga tgaaacgaac ttgaataaag aaaacagtga gatatgggtc tcacatgctg
     4801 cactaaataa agcattagcc gcatctccta cattccatac tgtttctacc cgtattgacg
     4861 aattatatac gactcacgcg tggactcaag gtggaaaagt aaaagaatta cccggagata
     4921 tcatcttttg ggcattgcga gatgtggtaa gcgaatctgg ttattaccaa aacaatgcaa
     4981 aagaaaccaa tgatttttgg aaaaaagtca accaagaatt agaagaagcc tatcacaagg
     5041 gaaccatcaa aaagaaaaaa gagttctatc tgatgggatc aggagatgga aagacgatga
     5101 gtgatctgcc aatcgtattc gatttccttc aatcaggata cgcttatgca tggcgatata
     5161 aagatttcca tcaaggagga aattatagtt tcggaacgca agaacaagtc aaacaggctc
     5221 aagaattatt aaaccttccg ctaatggaga attggatcga tagtaatgaa ccgagaacac
     5281 ctaatttcca attaacaaaa tcagcaaaag tcgcgaatgt catcataaaa atttaccaag
     5341 ttctggctat tcccgtgctg attgcttcaa tagcagggat attacttatg atactaggaa
     5401 ccgtatttgc aaaagctgat cgtgcctatt atagttcctc tttgattgtg gtcaccggat
     5461 taatactgac acaattggtt tttcttttcg gtgtctcatg gttttgttct tatgcgccag
     5521 aaaaaaagga ctatttttta agtgtctaca ctggtgcagg cgtgccatta acgcaattca
     5581 tcgtagtaac aagcatgcta gttttcagta aattaaatct gaaaaaagct ttatcttcta
     5641 aataaaaaga cccttggtag ctagattttt aacctaacta tcaagggtct ttgtgtggaa
     5701 aaatcagttt ttattcgatt tcttctaccg tttcctgcaa aagctgttca tattttttgt
     5761 ttcctttata taaatcacca atcgtccatg ccaacaggag aaggacaaga acaatgatta
     5821 cataagcgat gtgatcagcc aatgctagtt gagcggaagt ggcttgctcg ccgaagaaac
     5881 tttcgatctg tcctggtaat cctcgtaagt tcaagtaagt tagactaatc acagagaacc
     5941 agccaagaag ttttacgatg aatgaatttt taaatcggct acccatctcc gcttggctgt
     6001 tggtcatcat cagtaaagga atcatcgaaa atggcaacgc aaaagccaag aacacctggg
     6061 attcattcat taagttattc aaagcggcat gttcttcgat cgttcctttt gtacttgtaa
     6121 acaaaacaca aatcaataca ggaatcacag aaagcaatcg tgtgaccagt cttcttagcc
     6181 aaatcgggat acgcatatgg atgaatcctt ccatgatgac ttgaccggtc aacgtaccag
     6241 taatagtaga gttctggcca gaagcaagaa gtgctacagc aaataaagtg gacaagatac
     6301 cagattttgc tacagcaatc aaaataccat tgctcatagc tgatggatca gataaagctt
     6361 catacagacc aaaaaaggaa ggatcttgga cagaaccggt tttgaagaca gcgacaccca
     6421 tgatcaatag cagtgcattg actagaaaag ccattgacaa ctggatatta gaatcccaag
     6481 tagaaaaacg tacagcttgt gctacatctt cttcgttttg gtgatcgatc ttccgagttt
     6541 gtgaaactgc tgaatgaaga tataagttat gcggcatgac tgttgcacca ataatcccta
     6601 aagctccagt cagcggagtt tcgccgccga tgaccggtga gtctgagaat gtatgactgt
     6661 tagggataaa gccagcaaat aaactgtgcc aatctgggtt agacaagcca acttgataga
     6721 caaaaacgaa taaaatgacg aaaattaagc agacaaccag tgcttcgatc ttacgaaacc
     6781 cgacttttgt cagtagtaag agaacgaaaa catctaatac agtaataaag acggcaatca
     6841 ccaatggaat atcaaaaagt aagtagaggg cgattgctgc accaatgact tctgcgatgt
     6901 ctgttgccat aatggctaat tcagtcaaga tccataaaat gacacccaat gtcttacttg
     6961 tccgtgctcg gattgcttgg gccaaatcca tttgggtcac gattcctaat tttgctgcca
     7021 tatactgtaa aagcatagca attagactag acatcaaaat aactgacatc aataaatatt
     7081 ggaaattttg tccaccagtg atcgaagtgg accagttacc tggatccata taaccaactg
     7141 ctactaatgc acctggtcca gagtaagcaa gaagtgtttt aaaaaaactt tttccctttg
     7201 gtacatccac tgttccattt acttcagcta aagataaacc atttgtatgt tcaataagtc
     7261 gatgcttatg aaattgtttg ttggattgat tattcaagaa aaaaccacct ttcctattta
     7321 tctacgatta cattctacac ctcagaaaga aaaaattaaa gaaaaagatt cggtatgcct
     7381 aatttttttt cgtggtgtgt cttgctggtt aatgaaagaa attttgttct tctctaaaaa
     7441 aaccactatt ttttgcatga aactaaagaa tacgcctttc ttttttcaat aaactttagt
     7501 aaaataaatc attgagagca attcggaaat agaaaggaga gatttatatg gtatcaacag
     7561 gtattgtcgt acttattgct attattgctt tgctaatcgg cgcagtaggt ggctttttcc
     7621 tagcgcgtaa atatatgaaa gactatttag aaaaaaatcc tcccgttaat gaggacatgt
     7681 tgcgatcaat gatgatgtca atggggcaaa aaccatctga gaagaaaatt cgccagatga
     7741 tgcagcaaat gaagaaccaa gggaaaaaat aggttctgac ccaaagactt tttgcgggct
     7801 gattttttca gtccgttttt tatttcgtaa tttgttagac tattcagact attcagcaaa
     7861 aaagacaagc acagacaatc ctatgctctt gataaagaca aaggaggagt aaaatgtcga
     7921 tttttagaaa attaggttgg ttttttcgtc aggaaaagaa acattattta attgggattt
     7981 tctccctatt cgcagtggcc ttggtacagt tagttcctcc aaaagtgatt gggatcatca
     8041 ttgatgagat cgccgataag aatttatcaa taaagatcat ttcctggtgg atcttattat
     8101 tgatccttgc agctttctta caatatcttt ttcgttatat ctggcggacg aatatttggg
     8161 gaagtgcagc tcggttggag agagaacttc ggcagcagct attcaaacat tttacagaaa
     8221 tggacaatct cttttaccaa aaatatcgga ctggagattt gatggctcat gcaacgaatg
     8281 atttgaatgc aatccagaat gtagcaggag ctggaatctt gacctttgca gattcagtga
     8341 ttacgggcgg aacaacgatc attgcgatga ttttgtttat tgattggcgc ttaacactga
     8401 ttgcgctgat tcctctgcct ttgctagccg ttacttcacg agtgctgggg tcaaaactcc
     8461 acgatgcttt tcgtgattcg caagaagctt tttctagtat caatgataag acacaagaaa
     8521 gtatcactgg gatcaaagtc attaaaacat ttggacaaga aaaagaagat ctcgcggatt
     8581 ttgaagaaaa gattgaagat gcaattatta aaaacaaacg agtcaatttt ttagactcct
     8641 tatttgatcc attcattacc ttgattatcg gtctgtctta tgttgcaaca attattttag
     8701 gtggaaagta tgtcttggaa ggcacgatct caatcgggca gatggtctca tttattagct
     8761 atatcggtat gcttgtttgg ccgatgtttg cgattggtcg attgttcaac gtattggaaa
     8821 gaggaaatgc ttcttatgat cgcgtcaatg aacttttgca tgaaaagacg catatcgtgg
     8881 aacaactggg tgcagtgcag accccggcaa aaggcgagct gaatgtagct atttcctctt
     8941 ttaactatcc gaaagaagaa tcaccagcat tagaacaaat caaatttgcg atccaagaag
     9001 gagaaacatt aggtattgtt gggaaaactg gtgcgggaaa aacgacaatc atcaaattac
     9061 tgatgcgtga atacgatgat tatcaaggga aaatccaatt tggcggacgt tcgatcaaag
     9121 aatattcatt gaatgcttta ttaggatcaa tcggttatgt gcctcaggat cacttcctgt
     9181 tttcgatgac cattcgagat aatatccgtt tttctaatcc aaaactttca gaagaagaag
     9241 tagagcatgc ggcttcttta gcatttatca acaatgaaat caaggaattg ccaaaaggtt
     9301 atgatacctt agttggagaa cgtggagtct ctttatctgg cggacagaaa cagaggattt
     9361 cgattgctcg agcattgatc aaaaatcctg aattgctgat cttagatgat gcactttctg
     9421 cagtagatgc caaaacagaa gaagctatct tgcataattt aaaagaaata agaaaagaga
     9481 aaacaaccat cattgcagct caccgtttaa gcagcgtgat gcatgcaaaa gaaatcattg
     9541 ttgtcgatca agggaaaatc attgaacgag gaacccacca cgagctgctt gcattaggtg
     9601 gctggtatga acgaatgtgg gaaaaacagc aattagaagc gaagatagaa ggaggcgagg
     9661 cctgatggat gaaaattatc aatcggaatg gacaaaatcg attccactta aagaacagaa
     9721 gaagatcatc aaacgactga tgaagttcgc caaacctttt agaagaacgt tcatcgttgc
     9781 tatcttattt gcgtttgccc tgtctgtgat caatgtccta ttgccaagga tcatccaaac
     9841 ttttatggat gatcatctgg ctaaacagtc agctacgaca caagttattt tgttttttgc
     9901 tggtgtgtac ttgtttggag tcatagtcaa aagtatcatt tggtttttcc aatggtattt
     9961 gtattccatg gcttcattga agacctatca gtatgttcgg gtaagattat ttgaaaagct
    10021 gcatacacta ggtatgcgtt attttgacca gacaccggca ggatcgaccg tttctcgagt
    10081 gacgaatgac acagaaacgc tatttgaatt ttggtatgtc tttttgatgg tattgactgg
    10141 gatctttgca gtcgtttctt ctttcgttgc catgtttcaa atcaatggag aaattgcctt
    10201 gtggactttg gtgttcttgc cgattttagg aatcgtcatc tggtattacc agaaattcag
    10261 ttctcgtatt taccgaaata tgcgggaaaa attaagtcag ctgaacacga aattgaatga
    10321 atcgatttcc ggcatgcgta ttatccagca gtttcgccaa gaagctcgtt tagctaaaga
    10381 atttgaacaa gtgaacaacg agtatttaga cacgcgctat gcgatgatca aaacgaattc
    10441 gattctgtta agtccaatca tcaattttct ctatgcattg gcgattgcgt tggcgttgac
    10501 tttgtttggg atcgatgcgt tgcattctcc cgtagaagtt ggactgatct atgcatttac
    10561 gacctatgtc caggaatttt ttaatccaat gtctcaaatg atggactttt tgagcacctt
    10621 tacagatggg atggttgccg gtagtcgtat tttgaaaatc atggatacag aagaatatgc
    10681 accgcaacaa aatcctggag cagatcaagc gattacttca ggtaagatcg agtttcgcaa
    10741 tgtaagcttt tcttatgatg gagagcacaa tgtattgaac aatatctctt ttgtcgctaa
    10801 tccgggtgag accgttgcat tagtggggca caccggaagc ggaaaaagct cgatcatcaa
    10861 cgtattgatg cggttttacg aattttatga aggccaaatc ttgattgatg atcatgacat
    10921 tcgagaatat ccaatggaag agttacgtaa gaaaatggga ttggtcttgc aagatgcctt
    10981 cttgttttat ggagatattg caggaaatat ccggatgatg aatccagcta tcacagacga
    11041 acagattcgc catgcagctg agtttgttca agcaaacaag ttcatcgatg aattaccaaa
    11101 aggatatcac tcaaaagtga tcgaacacgg tgccagctat tctagtggac aacgacagct
    11161 gatcactttt gcgcgaacaa tcgtgacaga tcctaaaata ctagtattgg atgaagcaac
    11221 agcaaatatc gatactgaaa ccgaaggatt gatccaagaa ggattggcaa aaatgcgaca
    11281 aggacggaca acgatagcga ttgcccatcg gctgtccacg attcgggatg cagaattgat
    11341 tttagtttta gacaaaggtc atatagtaga gcgtgggaat catgaaacac ttctagcgca
    11401 aaatggtttg tatgcagata tgtacaaact gcaaagcagt ggtgtgtaaa aagaggttgt
    11461 gacagtagca tagtagcatt ttgccctaag cgctagagtg aaaataaaac agagcttcgc
    11521 agattcttca tctgtgagcg ttagtggcgt agtcacccga tttaaaaaag acgacgacag
    11581 ctagtcaaaa gcggctaata accactaaga aagcgttgac aaaagcgttt gctttcgata
    11641 atataaagaa aacgatacaa cttggatagt gattgcctga tgcaagctaa acctgatgag
    11701 tctcccatgg ttccatggac ggctttttgg gtttttcttt ttgttttgga tacagcccta
    11761 tatatcatgg aaaggagcaa aacatgctga ttgagaaagt actgaataac aacgtcgtaa
    11821 taaccaggga tgaaagaaat caggaaatga tcgtgatggg aaaaggtttg gcattcaaaa
    11881 agaaagtggg tgacccgatt tttgactcat gcatcgacaa aaaatttcag ttgacaaatg
    11941 acgggctttc acaacagttc caagaattgg cacaagcgat tcctcttgag tacttagaaa
    12001 tttcttgtga aatcattgaa tatacaaaag aggctctaca agtccagttg aatgattctg
    12061 tgtatattgc attgacggat catttgtata cggctatcga acgtcacaaa atgggaatat
    12121 ttgtaccaaa tgtattgctg tatgatatca aacaactatt tcctaaagag tatgccatcg
    12181 ggaaaaaaaa cagtgaagaa aatctcggat aaataccaca tcaagttatc agaagatgaa
    12241 gccggattta tcgcattgca tttagtcaat gcacaaggca ttcaaagcca acaagacaat
    12301 acctacctgt tcactataac aatacaggat attctaaata tcgtccgcta ttactataag
    12361 attacatttg atgaagattc agtccatttt tttcgattcg tcacacactt acgttttttt
    12421 ctgacacgcg tcctagatgg agaaaaatca gaagtgggag aagtttctga ggaattgttt
    12481 caggttattc aaaaaaaata taaggatacg tttggttgcg ttgagaaaat agcagacttt
    12541 ttagccgaga cgttcaacta ccagatgtcc acggacgaaa aaatgtattt aacgatccat
    12601 actgcgcgtt tagtgcatga gaatcgttgt aaataaataa aaaaaggagg ataaattaac
    12661 aagaacgtat gaaataaggg tagtgacatt cagttggcta aaccttccac taataaattg
    12721 gagggaatag aaatgggaaa ataccaagaa ttagcgagaa aaatcgtaga aaatgtcggc
    12781 gggaaggaaa atatcaatgg tttgacacat tgtatcacaa ggcttcgttt caaattaaaa
    12841 aacgaagaaa aagcaaatga tgagatcttg aaaaatatgg acggtattgt aacggtaatg
    12901 cgagcgggag ggcagtatca agttgtcatc gggaatcatg tgccagtcgt gtttgaagag
    12961 gttataaaag caggaaacct tacttttgat gaggctgttt caacagaaaa aatgagaccg
    13021 tttgatatgc ttattgatgg aatatcgggc tgtttccaac cattccttgc tatcctagct
    13081 gctgacggga tgattcgtgg actgacagca ttccttgtct tcttaggcgc gtttgaccgc
    13141 ggttctggca catttgttat gtttgataat atcggtgatt ctgtattcca atttatgcct
    13201 gttattatcg gtttaacagc tgcccgtaaa tttaaagtaa atgaattcgt agggatgttg
    13261 cttggcgcag cattaatgaa tcctagcttg tcgctggaag ctttgagtgg tgcagcagaa
    13321 gcaccactaa cgaccatatt ttctggcact atttttgaag cacctattta tcaaacagta
    13381 ttcggcattc cctggattgc gcgtaactac gcttcttctg tcattcccat tatctttatt
    13441 gtgttgttag ctagccaaat tcagaaacca attaaaaagt tagtacctga gatgatcgcg
    13501 aatttcttcg ttccattctt caccgttctt attacaatgc ctttaggttt tttactcgtt
    13561 ggaccagtat tcacattcgc aactgatatt ttaatggctg gatttgaaac acttttagca
    13621 ctttcgccag ttatttatgg agcaatcgta ggcttcttct ggcaaattct ggtgatgttt
    13681 ggtctccatt gggcaatcgt accaatggga ttgatgcaat tttctgtgaa tggttggcaa
    13741 aatattatga cacctgttgc agtagtgtct tttggacaaa ctgcagcact taccgcactc
    13801 tactttaagc ttagaaatcc aaaagacaaa gcaattgcaa taccagccat tgtttctggt
    13861 attgtgggga ttacagagcc agctatttat gggtttacat tgccacgtaa aaaaatattt
    13921 atttttacct gtgtcggtgg tgcgttaggg ggcgcgtata gtgggttaat gaatttaact
    13981 tcttggaatc aaggtggact aggcatcttt acaattccca actatattag accagatggt
    14041 gatttaacag atgtgattaa tgtattgatt gggatcgcca ttgcgatggt ggtatccttt
    14101 gcgctgactt ttttcttttg gaaggaggaa gcagatgaaa cagatgatag acagaaaaga
    14161 tcaggtaaag aaattgtaaa aacaccgata caaggacaaa tcgctccact gaatgcagca
    14221 aaggatgcag cttttgcaca aggaacacta ggtcgaggaa tcttgatcta ccctgaaaaa
    14281 ggagaagtac gtgccccttt tgacggaaca gtcatgtccc tgtttccgac gaaacatgca
    14341 atcgggatgg tttctgaagc aggtctggaa ttgctgattc atgtcggatt ggatactgtt
    14401 cagttggaag ggaaatattt tgaaagcttg gtgcagcaag gagaaagagt gaaacaaggc
    14461 gatctgttgc ttcgcttcga caaaaagaaa atcgaagagg aaggatacag tttagaaaca
    14521 cctgtgattg tcatcaatta catggattat ttggatattt tggaaaatca aggggaaacg
    14581 gtcgggccag acgatgcact tatcaccgca ctgacctgaa cggttaggtg aaagaatgga
    14641 ctaggcttaa ctattctttt tagtccactt ctttattttc aaaattgaaa ggctctttca
    14701 tgatggctga gctgataggg cgtttatcaa atttttgtcc tacagaaatt agatggacta
    14761 aattctgttg ataattttct gcaaaaacac aactatataa cacgtctaga agataacaaa
    14821 tcgatgtatt gatcgtaaaa ttaccaattt tcgaatacag tcgttcgcgt gtcgtaagtc
    14881 gcaaagcggc tgtactcatt gaagatagcg ttgaatcacc gatactggtc aaagctaaaa
    14941 ttggcacatg attttgtttc aatatttctg cggtacgaag gatcacattg ttttcacccg
    15001 tataagaaat cagaatccca cacatatcac ttgtgcaatt gtacgcttca tagaacgatt
    15061 caccaaaagt ggtgacaatc gtgacatcca ttttgatgcg cttcattttc agcatgaaat
    15121 cttgagaaat caaagtattc gagttactgg cgaaaatcac gatttttttt gcttgaagca
    15181 ttagttgttt ggctttttgc agttggtcat gatgaatgag agaaagtgta tcttgaatcg
    15241 tcatctgttc taaagaagcc attttatttg cgatcgtcat aataccgtct gtggtggaaa
    15301 aaggaagatt cgcatctacg tcttgaaaat tcccttcaag ataggctgtt tcagctaaaa
    15361 aagcattacg aaaactgacc cagccatcaa aacccatttt ttttgctaca cggatgagtg
    15421 tggacgggtg gacataacat cgctcagcaa tctcttggat ggtcatttcg ccaagtgctg
    15481 gagaatcgct taagagaaac tgaatcactt cattttctga aggagaaaag gagatttctt
    15541 tgattttttc acgtaataac atgtagatac cttcttcttg tttttttacg taaatcataa
    15601 cttttttgaa aatgatttgt aaacggattt atgttatata gcttaataaa gacatttttt
    15661 gacaagatat ttacaaaaat ccagaagaaa acactaactt ttgtaaatgt tactggaaag
    15721 aaacgaattt ctattgtttg ttttgtttac aaaaaagata ataaaagtgt aaaaagtaaa
    15781 ggagtgagaa catggcaaaa ggaatcaaaa tcgctacgat tggtggaggc agcagttaca
    15841 cccccgagtt gatggaagga tttatcaaaa gatatgagga attaccgata cgagaaatct
    15901 ggctcgtaga tgtagaagaa ggaaaagaaa agcttgagat cgtcagtcag atgtctcagc
    15961 gtatgtggga tgcttctccg tatgaagtca aaatctatgc aactctggat cgggaagaag
    16021 cattgaaaga tgcggatttt gttacgactc agtttcgtgt cggtcaatta gatgcacgaa
    16081 tcaaagacga gcggatccct agttattatg ggatgattgg gcaagaaaca aatggtgctg
    16141 gcgggatatt caaagctttt cgtaccgttc cagtggtttt ggcaatcgtt gaagacatga
    16201 agcgtttatg tccaaatgct tggctgatta atttcgcgaa cccaagcggg atggtgacag
    16261 aagcagttat cagatatggc aaatgggata aagtgatcgg attatgcaat gtgccagtaa
    16321 tggcgatgat gacagaacca acaatgcttg gcaagaaaaa agaagagctg atctacaagt
    16381 ttgccggtct aaatcacttc cattggcaca aagtagcaga taataaagga aatgaccgca
    16441 cggcagaatt gattgatcgt ttatacgatg aagacaatgg attgccgaaa aatatctttg
    16501 acatcccgtt tttcaaagaa caattaaaac aaatgaacat gatcccttgt ggctaccacc
    16561 gctattacta tcgtgaagaa gaaatgctgg aacatgcttt agaagaatat caaacgattg
    16621 gtacaagagc gcagcaagtg aaagaaacag aagcagagct gtttgaatta tacaaagatc
    16681 cagaattgga tcataaacct gaacaattgc aaaaacgagg tggtgcttat tattcagatg
    16741 ctgcctgtga aacgattgct tcgatctatg gaaataaaga aacacagatc gtggtatcta
    16801 caaagaatga tggagctgtt cctgatctgc cggcagattg tgtagtagaa gtcactgcct
    16861 atgttggcgc acaaggcgca cgaaatgtag cgtttggttc ccttcctgct gctgaaagag
    16921 gctggttgca agtaatgaag aatatggaat tgctgacgat cgaagcagcc gtgactggtg
    16981 attatggaac agctttgcaa gcgtttacga tcaatccgct tatacgatca ggaaagacag
    17041 caaaacggat catggatgag ttgtttattg cacatcagca gtatttaccg caattcaagg
    17101 aaacaatcag ccggttaaaa gcagaaggaa tcgaagtcca agatgaactg gcaagagaat
    17161 tatcaagcaa tacacatgta atggaggaag tatgatggga ttcagaaaag attttttatg
    17221 gggcggagct acagcagcca atcaatgcga gggcggttat gatgaaggcg gacgtgggtt
    17281 agcgaacgta gatgttgtgc caatcggaga agaccgtttt cctgtcatta ctggaaaaaa
    17341 gaaaatgttt gaatttgatg aaaatcattt ttatccagca aaaattgcga tcgatatgta
    17401 tcatcattat aaggaagata ttgctttatt tgcagaaatg gggttcaaga cttatcggat
    17461 gtccattgct tggagtcgta tttttccaat gggcgatgaa aaagagccaa atgaatctgg
    17521 gttgaaattt tatgaggatg tctttaaaga atgtcaaaag tacgggatcg agccgcttgt
    17581 gacgattaca cattttgatt gtccgatgca tttgatcaaa gaatatggtg cgtggcgttc
    17641 cagagaactc gttggttttt atgaaaatct gtgccgagtc atcttcaatc gctataaagg
    17701 acttgtcaaa tattggttga cattcaatga gatcaatatg atccttcatg caccattcat
    17761 gggcgcaggg ctgtattttg aagaaggaga aaatgaagaa caaatcaaat atcaagcggc
    17821 acatcatgag ttggttgcaa gtgccatcgc aacgaagatc gctcatgaag tagatccgga
    17881 gaacaaagta ggctgtatgc tggcagcagg agcatattat ccatacactt gcaatcctga
    17941 agatgtttgg gcttctagaa agtcagatcg agaaaattat ttcttcattg atgtccagtc
    18001 acgaggagaa tatcccgctt atgcattgaa agaaatggaa cgaaatggca ttcagatcaa
    18061 aatggaacct ggggatgccg cattgctgaa agaacatact gttgatttta tctccttttc
    18121 ttattattct tctcgagttt cgactactga caacgaactc ttggaacaga ctgcaggaaa
    18181 tatctttgct tctgtcaaaa atccatatct ggaagcaagt gaatggggtt ggcaaattga
    18241 tccattaggc ttgagaataa cgatgaatga tatttatgat cgttatcaaa aaccactatt
    18301 tattgtagaa aacggcttag gtgcagtcga tacgcccgat gagaatggtt atgtggaaga
    18361 tgattatcga atcgactatc tggcagcaca tatccaggca atgaaagatg cagtagaatt
    18421 agatggagtc gatctattag gctatacgac ttggggatgt attgatttag tatcagctgg
    18481 tacaggtgag atgaagaaaa ggtacggatt catttatgtc gatcgtgaca acgaaggaaa
    18541 tggcacatta gcccgttcga agaagaaatc attcaactgg tataaaaaag taattgattc
    18601 aaatggagaa aatctttcga acgattgatc aatgatttat aaaagtctat tgtttttcag
    18661 cggatttttc gagtataata aagctattcg agaaacgcaa gcagaacaat agactttttt
    18721 tcgcattggc tgtaaagtat gcttacttga ttaagtaaag gagaatctac atatgtatga
    18781 tgtaatcgtt attggcgcag ggcctgctgg gatgactgct gctttatatg cttcacgttc
    18841 aaacttatcc gtattgatga tcgaacaagg cgctcctggc ggccaaatga acaatacggc
    18901 agaaatcgaa aactatcctg gtttcgattc aatcatgggt cctgatctag cttataagat
    18961 gtatgaaggt gtttcaagat ttgggacaga aaatgcttat ggaatcgtgc aagatatcaa
    19021 ggatcacggc agccacaaag aagttatttg tgaagataaa acttacgaag gaaaaacagt
    19081 cattatcgca acaggctgta tccaccgtaa attaggcgta cctggcgagg aagaatttgc
    19141 tggacgtggc gtttcttact gtgcagtttg tgatggtgca tttttccgta acaaacgtct
    19201 agtagtgatc ggaggaggag actcagcagt agaagaagcc atctatttga cacaatttgc
    19261 ttctgaagta gtcatcgtcc accgtagaga tgaattacgt gcacaaaaga tcatccaaga
    19321 ccgtgcattt gcgaatgaaa aaatttcctt tatgtgggat acagtcgttg acgaaatcgt
    19381 tggaaatgaa atggtagtga caggtgtgaa agcacgtaat gtcaaaactg gcaatgagtt
    19441 tgatttggca gcagacggag tattcatcta tgtaggattg gatccattaa ctgcaccatt
    19501 tagagaagct gggttgacga acgaagccgg ctggattcca acagacaacg aaatgaagac
    19561 gaatatacca ggcgtttttg ccatcggaga tgttcgtgaa aaagatttgc gacaaatcac
    19621 tactgctgtt ggtgatggtg ggattgctgg acaacaagtg tataaatact tggaatcttt
    19681 agaagaaaca actgcataag tttaaaaaga gtgaaagaac ggtttcaatt gttcttttac
    19741 tctttttttt gcgtgaaatc tactgaatcc gatttatgcg aatacttgta accgtgcttg
    19801 ataaaagaat cttttcttga tttaagcgtt ttttggtcct catactttca tatctaataa
    19861 gtgaatgcta tacttattaa gaaccatatt tcagaaagag gtgttatttt catgtcttgg
    19921 gaacaagtgt atgaacaatg ggtacatgaa gaaaatttag aagaaaactt aaaaaaacaa
    19981 ttaaatgatc tgggtgaaga tcctgaaaaa cttgaagatg ctttttatgc accgttggaa
    20041 ttcggtactg caggcatgcg gggaatctta ggacctggga tcaatcgaat gaacatctat
    20101 acggtaagac aagcaacaga aggacttgca cgtttcatgg atacacaagg tgaagaaaca
    20161 aaacgccgag gtgtcgctat cgcttatgac tcacgccatc aatcaccaga atttgccatg
    20221 gaagcagcca aaacattagc acatcacaat atcccttctt atgtattcga aagtttacgt
    20281 ccaacaccag agctttcttt tgcagtccgt catttgaaga cgtttgcggg aatcatggta
    20341 actgcatccc acaatccagc agcttacaac ggatataaag tctacggaga agatggggga
    20401 caaatgcctc ctgcagacgc agatgcattg acgaaatatg tccgtgaagt cagcaatccg
    20461 ttaaaagttg aagtattatc tgacgacgaa gctgaacata gcggattgat cacgatcatc
    20521 ggagaagaag tggatgccgc ttatctagaa gaaatcaaag cagtaacgat tgatcatgaa
    20581 ttggtggaaa caatgggtaa agatttgaag cttgtgtaca caccacttca cggaacagga
    20641 aaaatgttgg gcgaacgtgc gttgaaacaa gctggattcg aacaattcgt actagttcca
    20701 gaacaagctg tcgcagaccc ggatttcact acagtcaaat caccgaatcc agaagaacac
    20761 tctgctttcg agtatgcgat gcgacttggc gagaaagaag gcgctgactt actgattgca
    20821 acggatcctg atgcagaccg tttaggcgca gccgttcgtt tgccaaatgg ctcctatgaa
    20881 gtattgacag gaaaccaaat cggagccctg tttgctcaat atattttgga agcacaccaa
    20941 tcgcgaggca ctttacctga aaatgcagcg gtattgaaat caatcgtttc tagcgaattg
    21001 cctacagcaa ttgcggaaca ctatggggct accatgttca atgtattgac aggattcaaa
    21061 ttcattgctg aaaaaattca acaatttgaa gaagatcatt cccatacttt tatgttcgga
    21121 ttcgaagaaa gttacggtta tttagttaaa cctttcgttc gcgacaaaga cgcgattcaa
    21181 gcattgctct tattagcaga agtagcagct tactataaaa aacaaggcaa aacattgtat
    21241 gacggcttgc aaacgatttt tgaaacatat ggctactacg cagaaaaaac gatttctgtg
    21301 acaatgagcg gacaagaagg ctctgcaaaa atctctgctt tgatggaaaa attccgtgaa
    21361 gaagcaccga cagaatttgc cggagcaaaa gtcatccaaa cagaagactt caaagagttg
    21421 acgagaacag accgtgaagg aaacgtagaa aaactaacga caccgccatc tgacgtattg
    21481 aagtatacgc tggaagaaaa tggctggatc gctatccgtc cttcaggaac tgagccgaaa
    21541 atcaaatttt atatcggtgt aaaaggaaac tcttcagaag acgcagctga aaaagtagcc
    21601 gcttatgaag cgattatcaa aaaaatgaca gaagaataaa aaaacttgag actgatggaa
    21661 aaagtggtaa actttagaag tcagtctttt ttcaagttgg aggtgaatgg attgccagac
    21721 acgcaaaaaa caaaagtaga gaaagtcagc caactattca aaatgttgag tgaccccact
    21781 cgtctcaaaa ttctgctgta tctgaaagac ggggaacaaa atgtgacagc aatcacccaa
    21841 gcagtagaaa tggaacaatc tgctgtatcg catcaactgc gtttattaag agaaaaccat
    21901 gtcgtcaagt cccatcgtga gggaaaagcg attttgtata gtcttgatga ttctcacgta
    21961 ctggatattt tgaaccagac gctcaaacat gtggaacatc aataacgata ggtgcaaata
    22021 gagacaaact ggactgtatc aacgaatctt cttcgctgac acagtccagt ttttttaaga
    22081 aaaagacaag aaaaactgtt agtctgtttc ttcggtggtt tcagttccat aataactatg
    22141 atcttctagg tctaaagcat tcaaaataag tgaagctgtt tcttcgatag ataaagaagc
    22201 gacattgatt actacgcagc ccaatttttc ataaagatca ttcgcaaaat cgagttcagc
    22261 tcgtattttt tcgatatcgg aataagcagt atcaggattt aatccgtaag agcgcatccg
    22321 ttctttacgg atattgttta aaatatctgg gttattcgtc agtcccacga tttttttagg
    22381 atcgatctcc caaagctgtt tgggaatatg tgcttgtggg atcaaaggta gattggcaac
    22441 ctttaaattt ttgttagcta aaaacaagct caaaggcgtt ttggaagttc tggatacgcc
    22501 tagcaaaacg acatctgctt ctagaaaccc acgaggatct ttcccgtcat cgtattttac
    22561 agcaaattcc attgctttga tccgtttgaa atagttctca ttcaataaat gaagggcgcc
    22621 aggttgccga atgggcttga taccagttaa gcgctcgatt tctgatatcg gcggagtcat
    22681 cacatcaaaa ctaaataatt gatgttcttc acaaaaagaa cgcgtcatct cgatcaattc
    22741 ttgattgatc aatgtgtgta ggacaagggc tttttctttt agtgcatctt gcagtgcctg
    22801 catcaatgtt ttctcatctg tcacaaatgt tctgcggaat aaattgaact caacggtagg
    22861 atattgggcc atagtggctt gcgctaattt tgtcgcagtt tctccagcag agtcagagat
    22921 gacaaaaaaa gtaatgattt ctgtagaatc ggaagacatg gataaaacct cacttgcatt
    22981 ttttttatgt ttattatacc atactctaga atgtgtactt cgtaatcatt ttgatttgga
    23041 ggaataagtc atgaaaaaac agacattgat tgaacaatca ctagaaacat tgaaaaataa
    23101 cggttttaaa tatactaaaa aaagagaagc attgttaact tatttagtga agcgcaatcg
    23161 ctacgtatcc gctaaagaag tttttgacta tatgaatcag gaattcaaag gagtaagtta
    23221 cgatacgatt tatcgaaatc tccatgattt tgcagaaatc gatttactgg aagaaaccga
    23281 tttaaatgga gagatgaagt ttcgttttca ttgctgtcaa ggggaaatcg gccatcacca
    23341 tcactttatc tgtacggtgt gcggaacaac gaaagaaatc catatgtgtc caatgaatta
    23401 tttcgaagaa cagctcggtg gatgtacgat cgaagggcat agattcgaga ttttcggaag
    23461 atgtgaaaat tgtcaataag gatttaaata aaataacatt tgataatttt aaaaaaaagc
    23521 aagatttcct tgacggtcaa aacagcatta gatataatat ttacaggaca gtcttgtttt
    23581 ttgagatata tttcaataac aattacggtt actttttaag ctcggaggga gggaattaac
    23641 atgtcaaaaa cagtcgttcg caaaaacgaa tcacttgacg atgctcttcg tcgcttcaaa
    23701 cgttccgtgt caaaagcggg tactttacaa gaatctcgta aacgtgaatt ctacgaaaaa
    23761 ccaagtgtaa gacgcaagaa aaaatctgaa gcagctagaa aacgcaaaaa attctagttc
    23821 tgtgatgaat tggagttggt agtgtgtcac tactaagtag attaaacgat gacatgaaaa
    23881 cagcgatgaa agcgaaagat aaggaatctt tacaagtcat ccgaatgatc aagtcatcta
    23941 tacaaaacga gcagattaaa gaaggtcacg acttgacaga agaagaagaa ttaacggtgt
    24001 tgtctcgcga aatgaaacag cgacgtgatt ctttgcatga gtttgaagaa gcaggacgtg
    24061 acgatttagc tgaaaaagta aaaagtgaaa ttgtgatagt cgaaaaatac atgccggaac
    24121 aactttccga cgaagaaatt cgacaacttg ttcaagaagc aatcacacaa actggtgctt
    24181 catcaatgaa agaatttggc aaagtcatgg gtgcgatcat gcctaaagtc aaaggaaaag
    24241 cagatggaaa ccaagtaaat gccattgtga aagaactatt acaatcttaa ttttatcatt
    24301 taaaggctct tcgtcaatga gtgttggtga agaaatt
//
LOCUS       DAJSKS010000030        23625 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJSKS010000030 DAJSKS010000000
VERSION     DAJSKS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.031"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            339..752
                     /locus_tag="ON396_001029"
     CDS             339..752
                     /locus_tag="ON396_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCR3113130.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            1372..1536
                     /locus_tag="ON396_001030"
     CDS             1372..1536
                     /locus_tag="ON396_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113131.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            1948..2493
                     /locus_tag="ON396_001031"
     CDS             1948..2493
                     /locus_tag="ON396_001031"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3113132.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            2494..2811
                     /locus_tag="ON396_001032"
     CDS             2494..2811
                     /locus_tag="ON396_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR3113133.1"
                     /translation="MKEVRPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(2968..3132)
                     /locus_tag="ON396_001033"
     CDS             complement(2968..3132)
                     /locus_tag="ON396_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HCR3113134.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            3234..3755
                     /locus_tag="ON396_001034"
     CDS             3234..3755
                     /locus_tag="ON396_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR3113135.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            3742..4215
                     /locus_tag="ON396_001035"
     CDS             3742..4215
                     /locus_tag="ON396_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR3113136.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKFCRLKIGQNR"
     gene            complement(4222..4380)
                     /locus_tag="ON396_001036"
     CDS             complement(4222..4380)
                     /locus_tag="ON396_001036"
                     /inference="COORDINATES: protein motif:HMM:NF024064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HCR3113137.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            4473..5234
                     /locus_tag="ON396_001037"
     CDS             4473..5234
                     /locus_tag="ON396_001037"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR3113138.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            5454..6773
                     /locus_tag="ON396_001038"
     CDS             5454..6773
                     /locus_tag="ON396_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR3113139.1"
                     /translation="MVAYDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFG
                     KPKRSSINYAPAFSKMEPRASDSRKLDGLNTHFGIFDEIHEFTNYNLINVIKKSRGTR
                     KQPLIVYITTAGYVLDGPLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPR
                     LWIKANPNICLMNFVGMLDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKR
                     NNKTIDIETLKGKKCVGGFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDN
                     NQERIKAWEKVGDLTIIPGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELE
                     NAGFETNEIRQGFLSLGGPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWM
                     PSKQSKSRKIDGFAASLNSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            6789..8009
                     /locus_tag="ON396_001039"
     CDS             6789..8009
                     /locus_tag="ON396_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR3113140.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            8044..8721
                     /locus_tag="ON396_001040"
     CDS             8044..8721
                     /locus_tag="ON396_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCR3113141.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            8718..9911
                     /locus_tag="ON396_001041"
     CDS             8718..9911
                     /locus_tag="ON396_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR3113142.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            9926..10252
                     /locus_tag="ON396_001042"
     CDS             9926..10252
                     /locus_tag="ON396_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR3113143.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            10256..10591
                     /locus_tag="ON396_001043"
     CDS             10256..10591
                     /locus_tag="ON396_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR3113144.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            10588..10983
                     /locus_tag="ON396_001044"
     CDS             10588..10983
                     /locus_tag="ON396_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113145.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            10980..11351
                     /locus_tag="ON396_001045"
     CDS             10980..11351
                     /locus_tag="ON396_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113146.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            11367..11927
                     /locus_tag="ON396_001046"
     CDS             11367..11927
                     /locus_tag="ON396_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR3113147.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            12052..12363
                     /locus_tag="ON396_001047"
     CDS             12052..12363
                     /locus_tag="ON396_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113148.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            12387..12578
                     /locus_tag="ON396_001048"
     CDS             12387..12578
                     /locus_tag="ON396_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113149.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            12578..16426
                     /locus_tag="ON396_001049"
     CDS             12578..16426
                     /locus_tag="ON396_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR3113150.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            16427..17299
                     /locus_tag="ON396_001050"
     CDS             16427..17299
                     /locus_tag="ON396_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HCR3113151.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            17296..18345
                     /locus_tag="ON396_001051"
     CDS             17296..18345
                     /locus_tag="ON396_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113152.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            18349..19173
                     /locus_tag="ON396_001052"
     CDS             18349..19173
                     /locus_tag="ON396_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113153.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            19191..21251
                     /locus_tag="ON396_001053"
     CDS             19191..21251
                     /locus_tag="ON396_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HCR3113154.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            21268..21417
                     /locus_tag="ON396_001054"
     CDS             21268..21417
                     /locus_tag="ON396_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113155.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            21414..21860
                     /locus_tag="ON396_001055"
     CDS             21414..21860
                     /locus_tag="ON396_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113156.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            21862..21999
                     /locus_tag="ON396_001056"
     CDS             21862..21999
                     /locus_tag="ON396_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCR3113157.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            22034..22276
                     /locus_tag="ON396_001057"
     CDS             22034..22276
                     /locus_tag="ON396_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HCR3113158.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            22292..22489
                     /locus_tag="ON396_001058"
     CDS             22292..22489
                     /locus_tag="ON396_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCR3113159.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            22500..23516
                     /locus_tag="ON396_001059"
     CDS             22500..23516
                     /locus_tag="ON396_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HCR3113160.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
BASE COUNT         8211 a         4013 c         5234 g         6167 t
ORIGIN      
        1 gtacaataaa aaatactata ggtaaaaaag ataacatgag gagcttattt gttctagagt
       61 gtttgacaaa gtaacaaaat gcgacgatac caatacaaac gggaatccaa aataacagca
      121 tgctccacat agtcactcaa ccatctccta tacgctttat tatcaataga taatatcatt
      181 tttcgtaata aagataaaaa aataagtaag gcttctcatt aaaatataaa aaagacagcc
      241 gaccactgac tgcctatata agagtattga aataaaaagc tgctgatata ataaattcca
      301 caagtttatt atatcacata aaggagcggt ttgacttgat gcaattgtta cgagaggtag
      361 atttcaaaca gacaagatgt aatgcgagag atgtgctgaa gaactttcgg cgtttggagc
      421 ggatggcagg tcgctctttg atagatatta agtcgccgat tattacggat atgccgaagg
      481 cgccgaagca cggcaataag gcagaagacg caatcattca gatgatggat atagaagcgg
      541 agagagacgc gattctagca gccttgatgg ctcttagtct gattagtcgt cagatactct
      601 actatagctt ttgtgtgcca gatagcttct caaactacag aattagccgt gaagtgggtt
      661 attcagaaag aagtatacaa cggatgaagt cggaagctct aatagagttt gcagaagcat
      721 ataaacacgg aagaataatt gcttataaat aatttggcgg ctttttggcg gaatgatggc
      781 ggtttttagc tatttaccag tgatattatg gtagtgtcga aagattagtg ataggtctaa
      841 gacaaaataa taataaaagg aacatcgttt tattattgtt tcacaattaa gcttcgatag
      901 acagcaacgg aaatattaag aataaggatg tgaattttaa ctccttctaa attgttctta
      961 ttatctatca tccgttgctg tctattgttt ttttaattat tcacacgata actaaaggtg
     1021 agcgaagaga aatgattcca ttaataattt caatttttgc gctttgtctt aatgtctata
     1081 tgattggatt taaaaatggg caaaataaaa gatagtagta actaagaaag aaatttttat
     1141 attgtcactg tggcggaaag ggtagacgct aagcatgtgt gctaggtcaa tgcttcggca
     1201 actatgcaat gttcgattca ttgccagtga ctttggcaac cgaggcatcg gcggtttaaa
     1261 aatatagggg tgcgcaattt cgtacgcgtt ttgtgcatcg tgtaggttgc tattacatat
     1321 tagatcactc gttgagtggt ctttttattt tacataaagg aggtaacaac gatgtataga
     1381 ccacaatact tagaacagaa gtatgaagta atcactgtgc aaaacggtaa cggtgagata
     1441 gtacgaaagt atagaagacc aataaagagc gatacatata aacgaaagga aaacaatgaa
     1501 gttatttctt ttcgcagaag gagaaaagcc aaatgagaaa ctactggtat gtatcgctaa
     1561 ctaatgaata tcctcgaacc attgatgatt gttcagtgcg tgttgtgcgt tctgtacaaa
     1621 tcaaagggaa gtactctatt gtcgaaatgc taagagaagc tacaccaaac gaagtggata
     1681 aatgcaagct gatatattgc ggtcatggct attggaaaga cgagtatatc caacaaaaca
     1741 ttgagaggtg gatagataga tgagttacct cgaacatttg aaacgttgct acatgcattc
     1801 taagaataaa cttcctgaca gctacactgt agatgatgtt gctatacatg tattgaagac
     1861 tgagagccat agcagtcctg atggtagcag taaggagcag acgctcgcgt ggttcaagtt
     1921 ctttaagtgg attaaggagg aagagtaatg cacatgcaca taggagaaaa gatatataaa
     1981 ttgagacggc ttaataacga aacaatgaac gacttagtta aacaaactgg agtgtcaaaa
     2041 tcatggttat ctgatattga gtctggaaaa aagcaacgcg ttgattttaa taagatatgt
     2101 gcaatagcca aacattataa agttagctta gaatactttc gggatgatta tttggatagt
     2161 gatgaacacg aggatattat tcctttgaca ggcgaaacaa gagttaaatc agtgaaatcg
     2221 cttgaacaaa gcctaaagta tctgaataac aaacaagaac ctaaccttct agagatcaaa
     2281 ctaaaagata ctgactcagt accagaggtt tggtacaagg gtgagaggtt ggatgaattg
     2341 cctaaaggat tagtagatgt ctcgtatcat tggaagactg atgattttac taatgatgat
     2401 agaggagcga acgacatcac gattcaatac ttttctagct ttaatgataa gtatccagat
     2461 aagaaaacaa tcggacacaa gagagatatg taaatgaaag aagttagacc tagagacgaa
     2521 atagacaaac tatacaagac caaacgatgg cgagacctaa ggcaagtagt aatagctagg
     2581 gacttcggca tgtgccaaga gtgcaagcgt cgagggcgga acacaagggg cacgatcatc
     2641 catcacatag tcgaggcgag ggaagacctg tcactgttct ggtccgtaga taaccttgaa
     2701 tgtatctgtg tagcttgtca caacagagag catccagaga gatcaggcgg gaagaagaaa
     2761 ccaaaaccta aatcacatat cgttaaaatg tattcaactc ctgaaagata agtttgcagc
     2821 gaaatgaagg tagcccccct actctaaaag attaaagagt aaggcttgag aagaacggtg
     2881 ctgtccttcc ttcgtaaaaa gaccgctttt caagtttttt ggagaaaaag gaaaaagccg
     2941 accaatttaa gccggctttg gacgaagcta tttcttagtc catttgtttc ctttttgaga
     3001 agtagggggt aatcggtcgc ctggatcaat ttttacttct tgtccgcctt ggacatttcc
     3061 accacgagga cccacttctt tgtaggttcc ttttggtttg ttgtcttcgc cgggtttata
     3121 gagttctccc ataggaatcc ctccttaaca aatttcggca cagcactgcc gataacctaa
     3181 ttataaggat tgtgataacg atttcaatct atcttttgaa agaaggtgat attatgccgc
     3241 aaccagcgaa gagtgcaaaa ttacaattat taaacggaaa cccaaataag aagaataccg
     3301 aagaactccg caagcgagcg gccgcagaag acaaattaaa aatggctact gacaaaatca
     3361 aaccgccgtt atggctagat tcgctaggaa aggatacctt tgagtttatc gccgatgaat
     3421 tgctgtctgt ggatttaatc agtaatccgg acgtccatac aatggctcta tactccaatt
     3481 ggtattcgca atacgtttct ttagaaaaac agcttcgaaa actacaacga gagtacaagt
     3541 tgaactatgc gcttgcgaaa aaggaggcag aggcgagagg tgagccgttt aatgaaccta
     3601 atgaattaat tggtaatccg ctctctcggc agatggatac agcgtctcgg aatctccgtt
     3661 cttttggcgc tgatttagga ctatcaccag cagccagagc taagttagct attaagatgg
     3721 ctgatgatgg tggtgatgac gatgacgact tctaatattt tggatatgtc ttacacagaa
     3781 cgtgtggact attggcaaag ctatcttgag gagcaagctt cttggggtgg ctttttaaaa
     3841 tgtccatatc cggaattgtt aactacttgg tatgcggaac gattaatcga tggaagcata
     3901 ccagccagca aagaaaatat tcaagctgct aaacggcata tgcgtgattt gcagcgccaa
     3961 ggaacagatg attttccttg gatctttgac gaagaaaaag gtcaccggcc tattagatat
     4021 atcgaaaaaa aatgtaaacc aactgaaggc gactttggtt cgtttgtttt gcaaccttgg
     4081 cagcatttca taattggatc catgtacgga tgggtacatc gtgatacagg agagcgtcgc
     4141 ttccgcgagg ctcttatttt tgttggacgt aaaaacggta agttttgccg tttaaaaatc
     4201 gggcaaaatc ggtgaaaacc tttatttttg gctttctttt agtaataatt cgatagcttt
     4261 atcaagcaat ttagacatag gaaccatagt ttcttccgac atttttttta atccttcgta
     4321 taaatcttta tcaattgcgt tagaaattct aattctgttt tttagtccgt atttgtccat
     4381 gtgtgacact cctttttatt ttactataac atccgattcc accgcttgca actaccatca
     4441 attgatggta gtatataaaa gaggtgagta gattgaaaaa atacattatt tacaaacact
     4501 taaataaaat aaatggcaaa atttatatcg gtgtcacaaa tgatattggc agaaggtgga
     4561 ggagcggtgg gattgaatat aaaccaccaa agaacgaaac ccaacaccac agaagttttt
     4621 ggaatgcaat acaaaaatac gggtgggaaa attttgatca tttgattatt gaagaagact
     4681 taacgatgaa agaagctttt gagaaagaaa agttttatat tgaactttat gactcaacta
     4741 ataagaaaaa aggttataac atcgcaaaag gcggaaatgg cggaataata tataaagttc
     4801 accccaaagg aatgctaggg aaaaaacaat ctaaagaatt ttcttcaaat cacagtaaat
     4861 gggcaaagaa ccataagaat aattgtatga caaacggtga tgttgtatgg ggtgttactc
     4921 acgaacaccc aaaaggcatg ttgggaaagc accacagcaa agaatcaatt atgaaaaaga
     4981 aagcatatag tggtgaaaat gctgtaacct caaaaccaat tgtcgcaatt gaacgggatg
     5041 gaactaaaag agaatttcac agcgctaagc tttgcatggc ttactattcg attagtacat
     5101 gtgtttttta ccgactttta aaagatggcg ccccttatgt gataaatcct aaagcgaact
     5161 acagaaataa agaaaaaata ttagctatcg aaggaattat gttcaaacat aaagaagata
     5221 ccgaggtaag ttagtagatt gcgaaaggtt actaactacc gtaacgcgta ggagttgaat
     5281 aaatataatg ctcccaagag tgtccgacag cagataattt gctgaaaatg tacgctaaac
     5341 tgggccagaa ctgactgacc gatgaaaatg agggtgacct ccagagtgcg agataaaaag
     5401 ctcgcagata ataacaatcg aaaacatcac taatttcagg cctttccaca tacatggtcg
     5461 cttatgatga tgaacaaggc gccaacgttt acgtattggc aaatgctcgt gatcaagcaa
     5521 gcttgttgtt tgataaggcc gcagaaatgg tcaaacaatc gccggcgctc tttaagaaat
     5581 ttggtaagcc taaacgatca agtattaatt atgctcccgc cttttctaaa atggaaccac
     5641 gcgcctcaga tagccggaaa ttggatgggc taaacactca ctttggtatt tttgacgaga
     5701 tccacgagtt tacgaattac aatctgatca acgttatcaa gaaatcaaga ggaaccagaa
     5761 aacagcctct gatagtttat atcacaactg ctggatatgt attagatggt ccgttgatgt
     5821 cttattttga gcaaggtgtg gactgtttgg aacatttgga agatgacatc gatgaacgga
     5881 ctttctatta tctggcaaaa cttgacagtg cggaagaggc tgatgaccca agattatgga
     5941 tcaaagccaa tccgaatatt tgtctaatga attttgttgg catgctagat gactatgtta
     6001 aggataaaaa agatccaaaa gaatatgctg actggattac caagcaattt aacttgtttt
     6061 ccgatatcga tgagctgtca tttgtcgata tgcctaccat taaacgaaac aataaaacca
     6121 tcgatattga aacgctcaaa ggtaagaagt gtgtcggtgg ttttgacttg tccgaaacgg
     6181 aagactttac cgcagccgtt ttagaatttc cgcttgaaac aggcgaggta ttcattttgc
     6241 aacacacatg gatcccacaa gctagatttg atcgagataa caatcaagag cgtatcaaag
     6301 cgtgggagaa ggtgggagat ctaacgatta ttcctggtga ttacgtcaat tatgaatacg
     6361 tcttaaattg gtttgtagaa aattcgaaaa tctatgacat tgtaaaaatc aattatgaca
     6421 aggccaaggc gctacgatta aataaagaac tagaaaatgc aggatttgaa accaacgaga
     6481 ttcggcaagg gtttctatca ttaggtgggc caatgcaaaa cttcaaggaa atgctattgg
     6541 acggtaaggt gattttcaac aattccaagc tttaccgatg gtatctatcc aacgtcaagc
     6601 tggtgatgga tcgcaactca aactggatgc cgtctaagca gtccaagagt agaaaaatag
     6661 atggttttgc agcaagtttg aacagccacg ccgaagtgtt gaatatgttg gttaatcctg
     6721 tcggaaccgg gaaagtaacc tattactcga tttccgattt aatgaatatg taagaaaggt
     6781 gtggaggaat gagtatttta gatcgtttgc gttcttttgg ccgagcgaag ccgaaagcga
     6841 gcaaacaaga gtattttttg aatgacccgg gattgatacc gtatttagtc ggaaaagatg
     6901 aaatatcaga agggattttt tccgtaatta gccgtgtatc gaacgttttt gcgtctctcc
     6961 ctctcaaaat gatagatgtg gagtttggcc aaccggacga ctgtcctgca tacaacttgt
     7021 tgagcgaagg ccctcgatat tttacaaagt ttgatttttt ccgggacgtg gaagttttga
     7081 gaaactacca agggaatgcg tatgtgcaga ttttccgaaa tatcaatgga gaagtagcag
     7141 atatggcgtt agtaaaacct ggtgcttgcc atccagtgat tgatatggat agcggggagc
     7201 tttactacca agtaactgcg actgacaaag gcagttacaa gcaagttatc tatgtacatt
     7261 acatggaaat gcttcacttt aaacaaccga ggtttggcgg cttggaaggt acagacccca
     7321 caaaagtatt aacgaatacc ctcggatatg atcgagaagt ccgaaaaatc tctttaagtc
     7381 agcttaaagg aagtaatgaa gggctaaaag ttaagtttgc tagcaatatg gatgaagaag
     7441 ctaaaaaagc tacagttaaa aacattgctg atttttatcg acaaaacggt ggactacttg
     7501 tggaagaaaa cggtgtagaa atcgaacgtt tacaacgaga gctggtagac agcaagcttt
     7561 tagatactga taaagtatct cgctccagaa tcgctatggt ctacaatgtg ccggagcatt
     7621 tcatcgggaa taaccagtcg agttactcct ctcaggaaca gctcaatatg gaatttttga
     7681 cgtacaatct agtaccgacc gttaatcaat atgaagcgga actaaataag aaaacactat
     7741 cgagaaccga aaaagcaaaa ggttatcgat ataagtttaa tgtcgcaagt ttgctaagag
     7801 ctgacactca ggcaagggga caattctatc aaatcatgcg acgaggcgga gcatactcag
     7861 cgaatgatgt ccgaagattt gaggacttgc aaccgataaa taaaaccggt atggatgatt
     7921 accatatttc cggagaccta tatccaatcg atatggatcc aacattaaga aaaacaacct
     7981 cgtctaaaag cgtagccgaa aacggttagg tttttttagt ttgcaccgaa gggaggtgga
     8041 aaaatgaaaa aagtgacgtt aagcggcgat gtcgtggata acgataccgc gtggctttat
     8101 gactggtttg ggatcaattg tatctcacca gggaaaattt ctgccgctct tacagaagca
     8161 gcgggggatg aagtagaact tgatatctca tcgaacggtg gggatgtcct agcggcaagc
     8221 gaaatatata ccgctatccg tgcctatccg ggaaaggtat ccggaaatgt tgtgagtatt
     8281 gcagcaagcg ctgcaagtgt aatcgcttgc gcttgcgaac cgcttagaat ctcacctacg
     8341 gcacacatca tgattcataa cgcatgggtg accactagtg gcaacgctga ggaattaaaa
     8401 gccaatgcag aaatgttaag cagtgtggat gagtctattg ttaatgctta cgagatcaaa
     8461 acaggactcg atcggaaaaa acttgctgat ttaatggcga aagatacttg gttaaatgct
     8521 caaacagcag tagcagaagg gtttgcagat gaaattatgt ttgcagaagc accagtaacg
     8581 gtactcaatg cctctcaacc ggttattcca aaaaacgcag taactaagtt gaaaaattta
     8641 atactcaaag cggaagcacc gcaagaagaa acactcttac agaaaaaact aaaagcctta
     8701 aatggaggga aaaacgaatg aatttagaac aattaaaaaa tgcgtgggtc gaggcgggaa
     8761 gtaaagtctc tgacttaaat gcacaactca atgcagcatt ggttgacgat gaaaaaacag
     8821 aagaagatgt agtaagtttg caagcacaag taaaagcagc acgggctaaa cgggacggat
     8881 tgaaagagca agtggcaaat atggaagccg aacaagtctt aaacgtcaaa aaagaaccat
     8941 tagataaaaa agatgaaaac ttgaaaaaca agtttatcaa agactttaaa gcgatggtca
     9001 atggtgatcc tgctattatg gctactttga catctaatac ggatgaatct ggtaatgcta
     9061 tcggattgac tattcctgta gatgtgcaaa cgactattca tactttggtt cgtcggtttg
     9121 actctttgca agaatacgta aacgttgaaa aagtgaccac taccagcggt tctcgcgttt
     9181 atgaaaaatg gtctgatatt acaccgttga ctgctttgga tactgaagac ggtgaaatcc
     9241 cagcaaatga tgatcctgca ctttacttga tcaaatactt gatcaaacgc tatgcaggta
     9301 tttccacagt aactaacagc ttgctaaaag atactgccga aaacattttg gcatggttgt
     9361 ctaaatggat cgcgaaaaaa gtagttgtta ctcgcaatac aaaaatcttg gcagctattg
     9421 atggaatcaa agcggcacaa aagaaagatg ttacagatgt tgatggaatt aaagatatcg
     9481 taaacgtcca acttgatcca gctatcgaag ctacatctat gtttattaca aaccaagatg
     9541 gcttcaatgt tttagataaa gtgaaacgtg ctgatggatc ttacttgtta caaaaagacg
     9601 tagcttctgc aactggatat accttcttag gtaaaccaat caagaaaatt gcttctcgtt
     9661 tcttgccaaa caaaggtacg caagctactc ctaaatatcc actgtacatt ggtgatctga
     9721 aagaagccgt tacattgtat gatcgcgaaa acatgagctt gctgacaacg aatattggtg
     9781 gtggagcttt tgaaacagac accactaaag tacgcgtcat cgatcgcttc gatgtgcaac
     9841 tagttgatga tgaagcggtt gttttggcta cttttacaac tattgcgaac gagacaccgg
     9901 cgaaagttta aggagctgat tccttatgat tcttgatcct aaaatggatt taggcgaaat
     9961 caaaaacgca ctaaagattg ataccgatga tgacgatgtg gaagtaagcc gtgcggcaca
    10021 agctgcaatt gcatacatta gaggggctat cgggaatgat aagccctctt tttataagca
    10081 agaaaatgaa acggttgatc tgattaattt agctatcctg caattagcag atcattatta
    10141 caacgctcga tcagcaaccg tgagtgggaa cttgcgagag tacgatttag gttttacaag
    10201 cctaatcttg caactcaaag caagttattt gctttttgtg gaggaggagt agcgtatgcc
    10261 ccttatccaa acaggaaatt taaatcaacg catcaagttt gtccgagata cgacggttaa
    10321 ggatgaggac gggcaagttg tcccgacttc tacaaccatt cttacttgct gggcaagtgt
    10381 gcagacacaa cgcctgaacg atattaagac gtcgattggt acggctttgg aaggaacact
    10441 gacgttcatt atccgctacc aacaaaaatc agagctgacc aatgatatga aagtgcgttg
    10501 gaatggaaaa acgtttgaaa tcattacgat tacgaaaggc gagtttgcga aggacttcac
    10561 gacaatcatt gcaaaagagg tttcaaaatg agtgtagaag tcgatgcaac cgaagtgtac
    10621 aaagcgctta gggaagtaaa agcaaacgtt caacgagtgg aaagcccagc acttagaaaa
    10681 gctggggagt acgctcaaga agagttacga caaaacacac cttactggga tggaacgaag
    10741 tcaaacggta aacgtggttc gtatatgcaa gaacatgcta agaaccatgt ggttacaagc
    10801 tcgataaaaa acggattggt agaagtcggc tatgacaaag atgtttcttg gcggatgcac
    10861 tttatcgagt tcggaacaat caaacaacgt ccaaaaggtt tcgtacaaaa aacacaaaag
    10921 caaatcgaaa aacaagtaac acaaatcatt gctgacgaag taaaaaggag gctaggactt
    10981 tgaaaacggc agtatcacaa gtctattcaa ttctgaatag caatgaaaaa acaaagaaca
    11041 ttgattttta caccaatagt gttccggaat cagctcaaac agtacctagc cttccagttg
    11101 gcagaattac agagatatcc ggcaactatg aagattttgc aagcaacaat cctttgacca
    11161 ttcaatttaa cgtacaggta gatgtatggg tgtcaaccat gaaagaggtt gatgcctttt
    11221 attttgccct tgatgaggtt atgaggggga atggttggca atgcgcatac acggaacaaa
    11281 cagatgacga ggacttggaa ggtgcaaagc ggattatcaa acgatatgta gcaaatattt
    11341 cactaaatta aaaggagaga aaatagatgg caacagtagg attcgagagc gtcatttttg
    11401 gcgtaaaaac aggtgcaggc ggcactctaa aagaattagt agcagataag tcgaaaggcg
    11461 gagcgatcga agctaaaatt actggattag gcgcaacttc taacacaaca tacgcttcaa
    11521 acgtaccgtt cttcattgca agtaaagggg tttcgtcgcc aaaagttacg cttgacgtgg
    11581 cagacttaat ggataacggc atttacagcg aaatcattgg tgctaaaacc gtggatggtg
    11641 caaatgtaat tggttcagaa actgaagcgc cttacgtgtc ggtagtcatg gttacagcga
    11701 acaaagaagg aaaacgcttg ttcatgggat tgacaaaagg aaaattcagt catccagata
    11761 tcgacatgaa aacagctgaa gacaaagggg tagaattgca aaccgattcc atcgaagggg
    11821 aattcatttc tgatgaacgt ggctatgtat acttaacagc cgtagaatca gaaggaatga
    11881 ccttacaaaa attcaaggac ttggtaaata acaaagaggg ggagtagtta accctgcatc
    11941 tacaccaacg acagatacag ggacaccaaa agaaccagaa ccaaaaattg atacacaagg
    12001 ttagccattt ttggctagcc ttattttttg taaaaacaag gaggaaaaca aatgattgaa
    12061 ttgcaattga aacttgacgg aaagaaaaaa acattcaaac aacaagatat ttccgcacgt
    12121 gcaatgcgtg agtgtatcaa attttacgag aaagcggaaa aagcagacct aactgattta
    12181 gaagcgattg attcaatgat tgcaattaca gcagatattt tccaagatcc agcagttaca
    12241 tttgatgcta ttttagacgg tttgactgcg agcgagttag taccggcatt agaaagtgtt
    12301 tttgaacaaa taaatgaact gggaaacaat gaaaaaaagc agacggcgag caaaaagaaa
    12361 taagtttttc tgaagctagg aaagcaatgg atcaaatcta caaagattta atcgaagcag
    12421 gttggacgat gagagatgtg gacgaagccg actatcatta tttgttacac ctttttggag
    12481 aagtggagag tggcgaagaa tatgtagatg gtgctgattt catcaaacaa tttttatcgg
    12541 ctgaagactt agtaaaactt gaggaaggag gtaaataatg gcaggaaaag gacaaccggc
    12601 aggaaatatc aagctaggga ttagtttaga tagcactagt tttggtaaca cgctggacga
    12661 aatcaatgcg aaagtcaaac aagctgagtc gaatatgcgt gccaatctaa aggcttatga
    12721 ttcagcagga cgttcatacg aagcacttag tcaaaagacg aaagacttgt ctacggttat
    12781 ggaagggcaa aacgccaaag taagagaatt aacaaagcgc cgtgatgaag cgattagcaa
    12841 gtatggcgag gaatcgaaac aagttgctaa ccttaacaca cagataaaca atgctaccgc
    12901 aaaatataat gcttacagtc gccagttgaa cgacacaaaa aaagaattgg tgtattccaa
    12961 aacagccgtc aatgatttat ctaatgaaat caaagaaaat gaacgacaaa tgaacgccga
    13021 agtaaaagcg ttgaaagccg ctggtgatga atctggtgct tttgaagcaa aacaaaaagg
    13081 gctagccaaa caaacggaat tatccgagaa agctatcgaa gaacagcgca aagttgtgaa
    13141 actgatggct gatgagtttg gcgattcagc aaatgaaacc gaagatgcaa aaagggcatt
    13201 agaaaagtta gaacgacaaa gccaaatatc tagcaggcaa ttagaagcac tcaaaagctc
    13261 cagcgatcaa tcaggaaaag aaatagaaga ttttggcgac aagtccacaa ggtcagctag
    13321 gaaactggac ggactaaaag acaaattaag ctcgctaaaa agcgcatttt cgtttggtgc
    13381 agttgctgga ttagcgcata acgctattag cagtgtagta agtggcgtgc aaggtttggt
    13441 tggcgaagca gtaaacgcat cggattcatt gatgaagttt tccaaaacca tggagtttgc
    13501 taactttggg aagtcacaga tagaaagctc gaaaaaagaa atgaaagact acgccgataa
    13561 gacggtttat ggtttagaag aaattctgaa cacaaccgca caattggcat ctaatgggat
    13621 tcctaactat acagaactaa ccaaggcggc aggtaacttg aatgccgttg caggcggttc
    13681 tagtgataca ttcaaatccg ttgccatgat gctaacgcag acggcaggag ctgggaaact
    13741 aacaactgaa aactggaatc aattagcaga tgcgataccg ggtgcttcag gattgttaca
    13801 agacgctatg ttgaaaaacg gagcttatac aggaaacttc cgtgatgcaa tggcgcaagg
    13861 tcaaatcact tccgacgagt tcaaccaagc aattgtacag ttaggtatga atgacggagc
    13921 agttaaggca gccacttcta cagacacatt gagcggttct tgggagcaga tgaaatccac
    13981 tgtaataaat gggctacaaa gtattataga aaaaataggc gttgaaaata tcactggttt
    14041 catcaacaga gtaacaaaag ggattgaaaa ttctattcct aaaattactc aatttatagg
    14101 ttggttgaga gatattggaa cgtggatcgt tgaaaataga gagccactaa catggattgt
    14161 cggaatcata ggcggaatta cattagcagt aaaagcattg aacgtagcaa gtatgttgct
    14221 ggcaattact ggcggaacat tggcagcccc ttttgtggcg attggtgtag cattaggcgc
    14281 actagcaggt gctttggtga tagcttatac aaaatctgaa acatttagaa atatagtcaa
    14341 tgcggctttt acagctgtga aaaacgtagt tatgagcgtt atcaataatt tggtggaata
    14401 ctacaaaatg ttgtggagcg tgttgcagtg gctttgggaa aaaataaaag aatgggcttc
    14461 atggattggc aataaattca ttgaaatgaa gaacagcgtt gtgaatacgg ttcagaacct
    14521 atggaatggc gtgaaaaact tcttcagcaa tggcgttgga gatacgtgga ataaggtagt
    14581 cggttgggta aaaaacattt tcaacaaagc aactgaattg aagaacaacg tttctgatgt
    14641 aatcggtaac ctgtggaatg gtatcaaaga cacattccgt agaggtatcg atacggtatt
    14701 caattggttt tcagaactac caacgaagat gaagaatgcc attattggcg gtaaaaacgc
    14761 cattgttgat gcgttcaaaa gtattttcaa cgcagcactt aaagcgatag gtaaaccagt
    14821 taacgcaatc atccatggag cttcatgggt actagaaaaa ttgggcgcag aacctctaca
    14881 agaatgggat gtaccacaat acgctacagg tacaccagca ggtgggcacc caatcaatgg
    14941 tccaatgatg gttaatgatg gacgtggagc agaaacagtt atcacaccag atggtagagc
    15001 cttcattcct aaaggacgca atgttgttct aaacgcacca aaaggaacgc atgtcttgac
    15061 agcagaagag accgcgcaac ttcaaggttc taaagctcct aagtatcgtt acaaaaaagg
    15121 tactaacttc tttggtaaca tgtgggatag tgtgaaaaag gttgctggta atgtaggtca
    15181 cacaattaaa aacgtagtag gtgacgtgtg ggactttatt tcagacccgg gagcgttagc
    15241 tagaaaagta cttggggggt tagatgtatt aggtggattg acaaagtatc cattagaagt
    15301 aggtaaaggc atcctatcta aagcaacaag tgcactgact gaaaaaatca ctgggttgtt
    15361 ctcatctggt aacttagata cctccgtagg aacaaatggc gtctataaat atttggctga
    15421 tgttgctaag tctgtgatga agaaattccc aggatttgtg gcaactagtg ggtatagacc
    15481 aggtgaccct tattcacatg gtaaacgtaa tgccattgat attgcattac caggcgtcac
    15541 aggaggctca cctagataca cagaagcagc aaactacgct tttgacaaat tcgcttccaa
    15601 aattggttac gtaatcacta atgggaaagt tcgtgaccgt tcaggacaat caggtcaacc
    15661 agcaactggt gcatgggagc catggcccga tggagatcac tatgatcatg tgcatttaaa
    15721 cggtgtgaaa gacccacaaa acactcaaat ttcaggagat agcgtgggag gcagtggggt
    15781 agaaagatgg cgcaatgtag caattagagc gttgaaaatg accggtcaat acagtactgc
    15841 aaacttaaat gcattactaa atcaaatgcg tacagagtca aatggtaatc ctaatgcagt
    15901 taacaattgg gatattaacg ccaaaaatgg aacaccatca aaagggttgc tccaagtgat
    15961 tgacccaaca ttcagacagt atgcaatgcc aggattcaac agcaatattt ttgacccact
    16021 atctaacatc ttagcttcaa tcagatacgc actatcaaga tatggctcac taacaaatgc
    16081 ctatcgtgga gttggttacg caaacggtgg aattgtaaac caacatcaaa ttgcggaaat
    16141 cgcagaagga aacaagccag aaattattat tccgttagat aaggctaaac gatcaagagc
    16201 gatgcaatta ttgtctatcg caatggacaa actaggagtt tctcctaaaa cttatggaaa
    16261 tgcgacaact gtttccagtg attacgaaat cctagaagcc attgaaaaac aggcggcaac
    16321 aacgaatcag ctgttatcgt tattgctggc atttttcaaa ggaaatagcc gaactgatag
    16381 agatttagct ttagatattc agaagatctt ggttaggagg atgtagatgc gaactgtatt
    16441 actgagaaat agaataaacg aagaaattga tttgtccaca gaagactatt ttgctactgg
    16501 gttgagtaat atggggtttg aagtaaaaaa agaacatgtg ggacagtggg ggaatttcag
    16561 agaaagcagt gagagcgttg aaatatctga atttcagtca tctgtaatca tttctgtgca
    16621 tgggtttcga gaaaaagaac tgtacaattc acttgtgcag ttcctatcgg aaggtccgtt
    16681 tgaactggaa tttgcttttg acggcgaaac aatggtaaga agatgcagtc taaaatcttt
    16741 aagtaagact gaaatcgatc cgaaaacatc gttactaaca gacactttag aactatattt
    16801 cacatcaaac tggtattccg taaaacgcga aaaattaatt cagcgaccga atgtagtgaa
    16861 aacacgtggt aaggtttttc cgtataaaag atcttatatc tacactcaaa acttgtggga
    16921 gaaaaaaggt gtattcaaat tcaataataa ttctgtatac ctaacgaata gcaaggaacg
    16981 gatgtccccg ctaaaaattc gtgtgattgg gaagtgttca aatccgtatt gggaagtaat
    17041 ccaaaactca caaatcatcg caacggatgg atacttcata gatatgactg aaacacagac
    17101 tctagaagta tcaagccttt ttgaagatac aacggcgatt ttaaaagata ttgcaggggt
    17161 agaatcttct gtctaccaac aacaggatta tacaaaaact aattttgttc aggccccaac
    17221 tggagaattc agcattgtgt ttcatgttgg gggggcagac gtagagattg agttatacga
    17281 ggagcgtgat ctgttttgat tttagcagtc acgctttttc atcgtgattt aatgctttat
    17341 aacgaacatt tattctctaa cagttttgaa tttggtgtgg acgagatcaa cgaagaggct
    17401 agtagcttta cgatggataa gtacgttcct gtaaaaacag gcgattttct actagcaaaa
    17461 tatattccta gtggaaaatt tgcatatttt ggtgtaatta cgtcgcagga agacgaaaaa
    17521 atcagttgta aaagcttact tagtttagct gatagtgaga taccgactgc acgtgtgtca
    17581 ggagataact acgaagagca tatccggcgg ttgattgaat actatttgct gaatgatcca
    17641 acgaaacaac taaaagatat tttagacgtc aaagcagaaa gtgcgacctc tcattcgtat
    17701 caagctaccg atacgaataa acacaagtta agtgcgtata ttctcaatgg tttcaaaaaa
    17761 tacaacgtaa aatggtattt caagggaatt caaaacagaa aaatttatac aggcatacgt
    17821 gctgtaaatg aatcaattta cattaaagac aattcttctg aatttagcga ttgggatgtg
    17881 tttgttcagg cgccgggcgc tggaaacgag aacaagctat taatcgttga taaagcaatg
    17941 aaagatatag agaaaccgat aatactgtca acatggtatt tggacgaaga aaacaatttg
    18001 acacaagatg gatcgaaaga aaatatcacg aaaccaactg tcaatttggt taatatctac
    18061 gatcaaaccg cagaagataa agcatcctac gaagatgtgg caaaatcaga gttgaaaggc
    18121 aatacgtatt cgcatgaaat caaagtgaac gttgtaagaa atgcaaaaaa tttgaatgtc
    18181 gaaacgattg aaacagggat gtttgccacg atttcttata aaggaaagat atacaagtcg
    18241 gttttgacag cttggcgaat atcaagtgat aaggagtttg tggaattgac tttcggaaac
    18301 ataagaagtc gttttatgga ttattttgaa gataatgggg gataaaaaat ggttagcaat
    18361 gtggatggat atcaatttga aaacgtgaaa gtaagcgcag aaaatgatgc tagactttat
    18421 cacgttttat ataatcggaa aaatcaggtt attgatggtt acgatcagtc tatgaattta
    18481 tcttcaagcg gattaacagt aaaagttgct gcaggagcag cgattattca aggtcgtatg
    18541 gtcgttgttc gacaagaaga aagtataaca gttccagcaa actcaagtgg ttatatagca
    18601 ttaacagtgg atttgacaca agaagttata cctggatcta ttcttccaga atcggaagaa
    18661 tatgaatgga ctaataatca agtcaagcta gaatttataa caaaagttat aaaaggtaat
    18721 ttaaacaatg gtgataaggt ctataattta ccactatgct cagttaattc tactggatca
    18781 actgtttcaa tctcaaagat atcggatagt tacgagctga ctctctctaa aggagaaatt
    18841 ttgtggaggg ggactgcgtt aatgcatgat actcaaactg tccaaccttc aaaaaaaatt
    18901 tggcagacag tttcaggttt tttgttagta tggctcccat acgaaaacgg gcaagcaatt
    18961 gaggatagat atgtaactac gcctttttat aaggagcgtg taacttttac taatgtttta
    19021 ggagaaattg tttcaggatt tgatgactat cacaaaaagt ggtttagtaa acgaataaac
    19081 tataattcga atactaatat atttacaggt gcagcaagca atgcaagtgg agataacgca
    19141 aatatggtgc ttaggtatat agtttctttt tagttaggag gtatggaaga gtggcaaatt
    19201 tagaaattaa attatctgca aataagagac agccttatct acgtcaccgt gttgttggta
    19261 gagttggtga tggggggctc acaacaatca atgtacaact tcttgaggaa gatgaaatta
    19321 caccttttgt aattaatcta aacggtactt tgaaatttgt gggcgaagtt tcaaacggta
    19381 actataccga gggagaacca gaaataatcg attcgactaa tgggttaatt agttacacgt
    19441 tcactaagtc aaatttcagc acgagtcatc aattcaaaca agcatatttt gaatatgtag
    19501 atcctaacgg caaaaaagta acttttcaga acttcatcat agacgtgtta gaacgagtgg
    19561 atattaattc ggagcaagcg aaatactata tttcttcatt ggaaaaatta cagagtgaaa
    19621 tgcaaaccac tttcaatcag ttcattagtg ataaacaggt ccaatacgat caaatctact
    19681 cgaaatataa cgaattagta agattgataa atgaatcaga taagcaagta aatgatcgta
    19741 ttgatcaaac caatcagcaa atcggcgatc tcggcaagct gaaaaagatg tacagtaaca
    19801 gcatcgactt tgggggctat gattattcgg ggaatccgaa tttgttaagt aagctatcat
    19861 acgacttaat tgaaaatcaa aatacttcag ctggaacact ttctaagggt gaaaactcgt
    19921 ttaaatataa taagatatca gctgaagtgg aaggtggagt agagttatat tataaacgaa
    19981 gaggtatagc taactggtta ccctctaata aaacgcttgt aatgactgtt aagcttagag
    20041 ctggagcgga ctatagtcca gttgacggaa aacttatact gattagatat aggtatgttg
    20101 acagtggaac tggcaagatt gttttagact tacctattaa cagtaattcg ataactcagg
    20161 aatggaaaga gtttagtatt actggaacta ctccaacatt tagcccacaa gcataccatc
    20221 cttggataca atttagggct caagatgggg tacttgggga aatagaaatg agctatgaca
    20281 tcaaaatcga agaaggctct acagctacac catatcagcc taacttactt gatgactctt
    20341 actggctagg taaaacaccg ttgggtgaga atttgataac gaatgccaag ttcccaatta
    20401 taacacgaag caatcctatt tctggttttg atatttcaga agaattaata ataggtgaaa
    20461 cttatacggt atctttaaaa gggactaagc cagctactaa agaattccac ttatactacg
    20521 gagaagcaaa ttaccagcag ttgcagtatc aggcaacttt acttcctgtg gaaggattaa
    20581 cagatgtgtg gagtgccact tttacagcca gaaatactag tgaaacgaca aatttactta
    20641 gagtagcgtt atggcaaaaa cctaattctg caacatatga tactgttcaa attgactggc
    20701 tcaagattga aaaaggcgac acacgcacac caaatatcag tcagtttaaa tactttggtg
    20761 aaggcttgaa agacagtaac aatccgaacg actacagctg ggatgtcaca ccagagtata
    20821 ctgaaaaaag cttgaataat acggttagtc tgactgaacc gcaatccatc gaaggattga
    20881 aaaatttcga agatggtatt caatcaaaag gaaaatctgt attgacatca gacgacaaca
    20941 aatatgaagt cgtaacctta acagttacaa acgggaatac cggatcagca aagctttatc
    21001 gtgaaggaaa aaccgtcact atttattttt ttgcgttgaa tgggaaaagc agtggtggaa
    21061 atgattcaac gatactaaca attccagaag gctatcggcc accaattagt tttgagcaac
    21121 tggttggctc gatagaccgt tctactttga acagtgctca gttatctatt ggtgcagatg
    21181 gagccattaa atggcgaaga aactcaagtt atggatcgga ttataccttt gcaattactt
    21241 acacgattta gaaaggcgtg aatcgatatg aaggcagcat atagaccaat tgaaccttac
    21301 ggattcgagc aaatcattgt gaatgatgaa gaacatttac cggaagaatg cacagaagtc
    21361 gaaccaccga ttccaaattg gaaaccgaaa ttcaattact gggagggaaa taaatgaaaa
    21421 acatttggaa atatggccgt actggcggag agtatgcagg aaaagtattg gacgacatgc
    21481 ttgtatccgt tccttacaca gatcagccac cgctcgaagg ggttcgttca gatggcgaac
    21541 cgctaacgat tgctgatcag atgtttgatc ctaaattgaa ccaatggatt attttagcga
    21601 acgcgttaga tcacaacgat ttaaacaatc tcaaagcgat gtacgaggct cttgaacatg
    21661 aaaacggcaa cctaaaacag ctaaatgcca aactcatgct aagcgatgta gcgattaaac
    21721 aggaaaatac tgcattgaaa gaaaaagctg acagtttagc acaaatcaat tcaaaaatga
    21781 tgcttgcttc gttacaaaat agcaaagaca tttcagaaat taaagagcaa ctaaatccag
    21841 cttcaaaggg aggtgagtag tatgtttagt tttagcgatg tgaaaatgat gtatgattgg
    21901 ggctgtttta ctgacgatca agttcgtcta ttcgttccac tatgtatcac agatgaagaa
    21961 gcagatagaa ttattagcaa agaagagagc gcatcttaag tgatgcgttt ttttgttgga
    22021 aagttggtgg aacatgaaag aagaagcgct ccaagacgtt gtggagagat tagtaagaat
    22081 tgaaacaaaa ttagacaact acgaatcact tagagaaaaa gctgatagtg caaaagattt
    22141 ggcagacaaa gcctactcag tagcgctaaa caacgcagaa gatatcaaag aaatgaaagc
    22201 caataataaa tggtcgtggg gttacatgat cggtttaggc attacgatca ttggctattt
    22261 cttgactaaa ttgtaaagga gttaagaaga aatgatttta cccgataagt attatcaagt
    22321 cattaaatgg acggttttaa cagtattacc agctgcatct gttttagtag ccacgttagg
    22381 aaaagcgtat ggatggaatg gaacagatat gacagtactc actatcaatg cagtagcaac
    22441 atttttaggc gttatcactg gtgtgtctgc atataactta aaaaaatagg aggaaaaaaa
    22501 tgaagaagaa aatcatttta tcattgagcc taataatggc tcttttttta ttgccaatta
    22561 acgggttcgc ctatacgatc aacaatgaat ttaatttagg tgtaaatgaa ggtagctcac
    22621 aagtagcaaa taaccaatac atcctattgc atgaaacagc aaacgaaact gcgacaggac
    22681 gaaatgaagc gcagtacatg aaacgttctt ggtacaatgc ctatacagcg tatattgttg
    22741 gcgatggtgg gatcgtttac caagttggac aacctggtta tgtacaatat ggcgctggtt
    22801 catatgctaa tgcaaatagt cctgtgcaaa tcgaactgca acatacgcac gataaagtaa
    22861 cgtttgaaaa gaactacaag gcatatgttg aattagcgag agattccgct aataaatacg
    22921 gtattccact tacattagac acaccgtata atcaaccagg aatcaaatca catttatggg
    22981 taactcaaaa catttggggc gatcacactg atccgtatgg atacttatca gaaatgggtg
    23041 taagtaagga aaaactggct tacgatttag ctcatggatt tacagacgaa aacccaacga
    23101 cttctgagaa caagcctgtc attgatccaa cccgagcagg tgcagcaaat ccaactttat
    23161 cagatggaac gaaccattct cacattgatc agtttggaga aatcgaaaat gcgaacttac
    23221 acgtcgctgg atggcacatc gctaactata aatacgagta tattttcatt atggactaca
    23281 atactgggaa agaattagct cgagtaagag ctgatggaat ttatagaccg gatgtaaatc
    23341 aagcttataa tacttctgga aacgttggct atcatgtatc ttttaacatg cgcaatttcc
    23401 ctaataagaa agtatacgtc atgatgcggg caacgaatga tccagaagga aacactaaag
    23461 gcggtgcgca agatttccat gacaaacgtt ggtatttaaa tattcctaaa cgataaaaat
    23521 agctcctcgt tgaggagcag tacatagaat tgaaaattaa cgttaaatca aaaaaatatt
    23581 tacaagatgt ttatttaagt agataataaa agaaaaaagc aggtg
//
LOCUS       DAJSKS010000031        23424 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJSKS010000031 DAJSKS010000000
VERSION     DAJSKS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.032"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(48..2192)
                     /locus_tag="ON396_001060"
     CDS             complement(48..2192)
                     /locus_tag="ON396_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR3113161.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2549..3922)
                     /locus_tag="ON396_001061"
     CDS             complement(2549..3922)
                     /locus_tag="ON396_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3113162.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3922..4317)
                     /gene="rbsD"
                     /locus_tag="ON396_001062"
     CDS             complement(3922..4317)
                     /gene="rbsD"
                     /locus_tag="ON396_001062"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR3113163.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4345..5262)
                     /gene="rbsK"
                     /locus_tag="ON396_001063"
     CDS             complement(4345..5262)
                     /gene="rbsK"
                     /locus_tag="ON396_001063"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR3113164.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5370..6383)
                     /locus_tag="ON396_001064"
     CDS             complement(5370..6383)
                     /locus_tag="ON396_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR3113165.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6464..7063)
                     /locus_tag="ON396_001065"
     CDS             complement(6464..7063)
                     /locus_tag="ON396_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3113166.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7249..7950
                     /locus_tag="ON396_001066"
     CDS             7249..7950
                     /locus_tag="ON396_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3113167.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7960..10671
                     /locus_tag="ON396_001067"
     CDS             7960..10671
                     /locus_tag="ON396_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3113168.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11122..12750
                     /gene="treC"
                     /locus_tag="ON396_001068"
     CDS             11122..12750
                     /gene="treC"
                     /locus_tag="ON396_001068"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR3113169.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12810..13991)
                     /locus_tag="ON396_001069"
     CDS             complement(12810..13991)
                     /locus_tag="ON396_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3113170.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14243..15766
                     /locus_tag="ON396_001070"
     CDS             14243..15766
                     /locus_tag="ON396_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCR3113171.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15835..16563)
                     /locus_tag="ON396_001071"
     CDS             complement(15835..16563)
                     /locus_tag="ON396_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3113172.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16576..17856)
                     /locus_tag="ON396_001072"
     CDS             complement(16576..17856)
                     /locus_tag="ON396_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR3113173.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17997..18599)
                     /locus_tag="ON396_001073"
     CDS             complement(17997..18599)
                     /locus_tag="ON396_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCR3113174.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18844..19050
                     /locus_tag="ON396_001074"
     CDS             18844..19050
                     /locus_tag="ON396_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113175.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19362..20081
                     /locus_tag="ON396_001075"
     CDS             19362..20081
                     /locus_tag="ON396_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR3113176.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20162..20845)
                     /locus_tag="ON396_001076"
     CDS             complement(20162..20845)
                     /locus_tag="ON396_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HCR3113177.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21064..23250
                     /locus_tag="ON396_001077"
     CDS             21064..23250
                     /locus_tag="ON396_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR3113178.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         7791 a         4220 c         4592 g         6821 t
ORIGIN      
        1 aattaaaaag ggagtgtgac agtgtcacat ccccttttta atttattcta tttctttaaa
       61 ggcagatcaa gccagaatga tttgtctaaa cttaacaagg ttagtacagg aacagttgca
      121 gctttaactg tacgagtagt tgtttctggt atctgtcttg ccgtaaaatt gttccacaaa
      181 tcccagattg aagcgacacg gaagatggaa gaagcgttgt tgacgcgttt tatcgttgaa
      241 aattctaatc ctgtgattgg cacgccatct gttgtccatt tgattggcac atcgaatcgc
      301 ttagacatat tgaggatttc agcgtttgct tcttcagttg ttgctgcttc ttttttcggt
      361 tcatcatatt ttgtcaggtc gtacgcttcg atgatcgcgt tcaatttttg tgaatattgg
      421 cgatctgtag cataacgtcc ttccaaatat ttggttgctt ctttgtagtc ttttgtttcc
      481 gacttccaag ttggtttgta gaaatcagga ttgccagtca gtccattttt gatcaagtcg
      541 gcatagtctt ccaaactttc cttgtaagaa ggatatttac ggaattgtgc gcggatttgg
      601 tagctttgtc cggaactatc ttgttcaagt gtattgaagg tcacgctgct tcctttatat
      661 gatcctttga taccaaaaag gttatgattt ggttcgcttg ataataagct gtttccagat
      721 ccactttcaa ggatagcttg agccatcatg actgatgcgt agattccttc tttttgcccg
      781 atttcctgtg catcatctgc aatctttttc aagaactgat cagttgtttg gttttgactg
      841 aaatggaacg agccatctaa tccatctaaa atagatgagc cgcctgtcag cgaacctgaa
      901 gcagcaggta aacctgctaa gtttactcgg acagcaaaat caggcgtgaa atctgaaata
      961 gaagttactc gaactttatc tcctggttga ggcgcatgga taaattgtcc gttcccaata
     1021 tagataccta catggtgagt aacaccggaa gaaggtcccc agaagacgag gtcgccaggt
     1081 tgggcctgac ttactggaat ctgcgtacct gcatattgct gttcgcctgt ccagccaccg
     1141 atataatgac cggttgcttt catatatacg taataagtca aaccagaaca gtcaaaagta
     1201 tttggacctt ttgctcccca tacatatggt tttccaagat gcttgaatgc ttctcgaacg
     1261 attgcttgtt ggacattcgt tgtatttgta ggcaatacag catcaattgg tgattttaca
     1321 acaaaatcag aagaagcaga agatggattc tctataccgc tttgatttgc tgtatttgga
     1381 cgagatgaag cattgttcgt tccgccattc gatgaaccat tgtttggatg gtttggttct
     1441 ctctgagtat ttccattgtt attcgatcca gatgggtttg tttgattcga agaattcccg
     1501 ctgttcgaat tattgttatt attgtttgaa ttatttgaac cattattatt gttgttatta
     1561 cccacatctg gtttgtttgg cgtttccgga tctggtgtac tagggacttc ttcactagga
     1621 tcagttggtt tctctgttga agtatccggt ccagctttaa attctgcagt aatagataaa
     1681 ttgttcgtac ctactgtaaa ggtctttgca tcataattta cttctttggc accattaaca
     1741 gcgactgaag caatttgttc accttcagct ggcgtaatag tgtaagttac cttcgtttcc
     1801 gctaataaac cttctaccgt tccactttcc gtaatatcca aaggttgctg ttcaggggaa
     1861 tccgctgtcg aagcagtcag ttggatctta ccaatcaaag aaggagcaat tgtaagggaa
     1921 tatgtctcgg ctggcttttc attttctgat ggatcagccg gatcaatttc agttggctcg
     1981 gtcgatcctg ttgttgagtt agttgttcca gttgggtctt ctgtaaaaga atccgttgtt
     2041 tcactagtag ctgagctgga tgtagtcgta tctgttgtga ctgtttgatc agctatttga
     2101 ttatctacgg caaatacggt attggtcgac aaaacagacg gagcaatcag ccaagtagca
     2161 gctagcaaac gtatcgtttt tttatttttc ataataaact agtccttttc ctttcattga
     2221 ttttctgtct tattcagcat tcattagaca ttttttggaa cagataatcg tgtcgaaaag
     2281 ctttttcttt tcataaatct gtctgtgtat tctaaataaa acaatttgta aataagcaac
     2341 gagcattcat tcgtttttct ttgacgttca ttttattgca tagaccacct ttgtttgggg
     2401 ataaacaagt ggggataaaa atggttatgc atatgtaaat tataccaaag tttggaaagt
     2461 atcacaaaaa tttataaaaa taatgatcct aaaaagaaaa agactgaaat atgtgataaa
     2521 catctcatac ttcagtcttt ttcattaact attcataaga cattaggtct tcatcaaacc
     2581 cgctaacgag agtagtgatc attgctacga ctagactaac agccagtcct gctaagtacc
     2641 agaaaaaagt accggaaatg aatgcaggca atgtggtcag actaccaaat acaaaagcga
     2701 tcgctttcac ttgggcaaat cccattacag ctccaccagc agctgcacca atgcaagcag
     2761 caacaaatgg ttttttcaat gggatcgcaa ttccgtaaac agttggttcg gtcactcccg
     2821 caagtaaaat cgaagcaaaa gcagaaccgg agatcgaacg ggtttttgaa cgtttacttc
     2881 gcagaaatac acctaaagct gcaccagcca ttccaaaatt ggctgctcct gtcaacggaa
     2941 cgattcggtc gaacccataa gcagagatat tactgatcat gacagggttt acagcccatt
     3001 ggataccaaa tattaccaaa atattccaac caccagcgat gattgcgcca ctgatcggtc
     3061 cgttaatatc catcatccaa gtaattgcgg cagctaaacc ttcgccaagg taaacacccg
     3121 caggcccgaa taccattaac gtcaaaggaa ccgtgataac taatgaaagt aaaggtacaa
     3181 atactaattg catattttcg cgaatatatt ttttgaggaa tttttcaaca aaagaataaa
     3241 agaaaatacc taaaatcgcc ggcatgacag tagacgtata ctgcatcagt actacaggaa
     3301 tccccagaaa atcagtagct gctttgccac tgtctgaaag taaagaggtc agattcggtt
     3361 caatcagtgc tgcagcaatg acagccgcaa tatatccatt cactttaaac gttttggcag
     3421 cagaaaaagc aagaaagatt ggcagaaaat aaaagacggc attcgacgca gcagacaaaa
     3481 tatgataagt tccgcttgtt tcactaagcc agccaatcgt agtcgctaaa acaaccagac
     3541 cttttaatat accagaaccg gcaaacagcg gaatgatcgg cgtaaagatt tccgatatca
     3601 aaccaaatag tttttcgatc ggatttagct tttctttcgg agctgaatga tcgatttgac
     3661 gcgtcaattc cttgtaagca ggagccaatc cctgatccat gattatttct gcatatctat
     3721 cgagtaaaat gaaatactta atggaaggga tttgctgttt taatttttct gcattggcta
     3781 ctggctgttt tttcaatttg atacgaagcc gagtcgcaca tggaaggaca gaatcaatat
     3841 tgtcttttcc tcccaactgt tcaatcagca tatgaatctg ctcggacagt accatctgtt
     3901 caggattcat tgtcgtttcc attagaagac cactccactc actaatataa tattggcgta
     3961 aggtgtcatt tcgcctgtac gaataaatgc atgacagtta ttcaactctt ttttcatttc
     4021 ttcatgtggg ataaagctaa tcggtgtatc tggtaacaat aatttgattt tttccaatat
     4081 ttccgggttc attgtcttta tttcttccgc caattcaatc gattgtacct caagttcttc
     4141 taaaacgttt tctaaaacag ataaaaaact tggaagaccg tttgagacag ccaaatctat
     4201 tttttcagta ccaaagggga caggcatgcc tgcatccccg atacttaatt tatcaaagtg
     4261 ccccatttgt gcgattactc gtgaaatatc tgaattgatt acttttcctt ttttcatgtt
     4321 tgtttcttcc tctcattgtt aattttagac tgctgattgt tctaaagctt cgcggtaagg
     4381 gatagaaggt tgcgcaccat aagactgcac agcaacagaa gaggctagat ttccataaag
     4441 gatcgcttct ttcaagttac taaggtctgg ctgaagtgtg ctgcttaatg cgccaataaa
     4501 tgtatcacct gcggctgttg tgtcaacggc atccacttta aaagcaggga cgataccgtg
     4561 ttctttttct gtatgataga aagcgccggc actgcctagt gtaatgatta ctgttccaat
     4621 acctaattca tggagttttt cggctgctgc gaccaaactg ttatgatctg tcactctgat
     4681 tcctgtaatg atttctgttt ctgtttcatt tggtataatg atatctgtct tttcaagtag
     4741 ttcagtagga atcgtttcac gagctggtgc aggattcagg atcgttgttt ttcctgcgtc
     4801 ttttgcaata gaaaaagcta ggatcgtcgc atctaagcta ctttcaaact gagcaataat
     4861 aaagtcactg tttgtaatca gttgcttgtt ttttaggact tcttgttcat ggaaagcatt
     4921 atttgcccct gcatggatca agatactatt ttctcctgct gcgtctacca taatcattgc
     4981 ttgtccggtt gtggcgcctt ggatttcagc tatcccagat gtatcgatgt tttcttttgt
     5041 cagtagatct aataactgtt gtccttcgct gtcagcccca acgcctccaa taaaagatgt
     5101 ccgtgcgcct gaacgtttgg ctgccactgc ttggttggca cctttacctc cgccagcgtg
     5161 aaaaatctca tgcgcatgca tcgtttcgcc tggcttgggc atgttggtta aacgaagggt
     5221 agtgtccata ttaatacttc caattactgt aaccgtgttc attttacaaa cctccaacta
     5281 tctcatttat tttgatggga aaattatttt ttaaaacgtt ttagtaaaac gttttaatag
     5341 ttcgataaaa atatagcaca ccttgaaagc tatttcaagg gtgcagttga aaatcttttt
     5401 tctaatcgta caggtaatct tttttcttcc cattcttttt ctgggaattg gatccgatct
     5461 aacaataact tggcagatgt ttgtcccaat tcgaagattg gttgtgcgat cgttgtcagt
     5521 ttcggcttga tatactcgca catatctata ttatcatagc caataatgct gtagtcttca
     5581 gggatgcttt ttccagcttc ctctaatccg cgatataatc caaaagctaa ttcatcattt
     5641 aaagcaaaca caccagttgc agcagaatca agaagttctg ctgtgatctg gtagccacct
     5701 tgttttgaga actgtgtagg taagaggatc agttggtcgt gagggatttg gtaaacatca
     5761 agagcagatt taaacccctc aatccgagcg tggacatttt ccggtggatt ttcaggatag
     5821 acaagggcga tcgtttgatg tcctagacta agcaagtgca tgcccgctag gtaaccaccg
     5881 cgaaaatcgt ctgttcgtac ggcatcacta aatccttcag attttttctg atccaaaaca
     5941 atgaagggcc ggccttgttt tttcaaattt tcgttgattg catctgtaga gacagcagaa
     6001 gtcgcgataa tgaatccatc cacacctcga cgtgtgagct cggctagata ttcgatttct
     6061 ttttgatgat cagaatcagc attacataat atcgtgacga aattctgctt atacaagata
     6121 tcctcaattc ctcgcatcag tgtactgaag aatggattcg tgatatcagg aacgagtaca
     6181 ccaattgttt tcgtttcctt ggtaatcatt tgtctagcaa aatagtcagg ctgatagcct
     6241 aattcttctt tggcagctaa aactttctga acagttttcg gactgaattt ctccgtattt
     6301 ccatttaata tcaaggagac agttgtgatt gaaacgccgg aaagtttagc gacttctttg
     6361 attgtgatct tttttttcac catgttcata ctcctatcta aatcctgtgg ttattttccc
     6421 atgaaatcaa aaagaagtaa atgaaaatca cgtgtgccag tacttatttg ctttgttgga
     6481 tagcttcgat tatttgtttc gttgagcgta ctcgtgcagt tttcggaaat atctttttta
     6541 tgaacaattg atgggattct ttgtcacgat cactgcaagc atcttcagca agcacgatat
     6601 ggtacccgtg ttgaaaggca tctaatgcag tagcatatac accgtttgtt gtcgctacac
     6661 cgcttaaaat gatagtttcg attccacgcc ggcgaagttg aagatcaaga gaagtaccaa
     6721 aaaaagcact tggattgtat tttgtgatag tcaacatatt atctgtatct ttcaaagaat
     6781 ccttcaaaag taaatcagtg aattctggag gtacagaagc agtcattcca cccatatcag
     6841 tttgttgact caaataaggg aaagtggaga tgtctacgtt cactaaaact ttcaaggcag
     6901 cggtacgttt caatgttctt actaacaaat cattgtttgt cagaatagat gctgcagtgt
     6961 gtggagagag tgtaccgtta tttacgatct ctttttgcat gtcgatcgcg acaaaagcag
     7021 tttttgataa atcaaaaatc atattttcct ttaaatcatt catgatgttc cttctttcaa
     7081 ataacaatgt tttttatgta agtaaatcat aaatcgttta tttgtcaact aaattaaagg
     7141 attcctttct ttagtctaga gaaattcagt ggtaaaataa ataagtgtga gaaaacgcat
     7201 tcgatgcata gatatagcga gaaatatcgt gaaaatggag gaatcagagt gaagtatgtc
     7261 gaagtaaaaa atgaatacaa acgatacaaa atgggagaaa cgaccattac agcaaatgac
     7321 gatatttctt tttcaattga aaaaggcgaa ttagtgatta tacttggtcc aagtggagca
     7381 ggaaagtcaa cgattcttaa tattttaggt gggatggata cacctgatga aggtcagatc
     7441 attattgatg acacagatat tgcgcaattt tcagataaac agcttactgc ttaccgacga
     7501 acagatgtag ggttcgtttt ccaattctac aatctcgttc ctaatctaac agcaaaagaa
     7561 aatgtggaat tggcaacgga agtttctcct gatgcgcttg atcctgtaga ggtattgagg
     7621 caagtgggcc tagctcatcg attgaataac tttccgtccc aattatcagg aggcgaacag
     7681 cagagggttt caattgctcg tgcactagct aaaaatccca aacttctttt atgtgatgaa
     7741 ccgacaggcg cgctggattt tgaaacgggc aagcaagtat tgaaattatt gcaaaacgct
     7801 agcagacaac acggcaatac cgttttgatc attacacata attctgcttt agcgcccatc
     7861 gctgatagag tcattcacat taatgatgct aaagtaaggt ctgttgaatt aaatgatcat
     7921 ccttcatcca ttgatgagat tgtatggtag ggggatttta tgaaaacaag aacatatctt
     7981 aaagcaggtt ttcgcgaaat tcggcaatct aaaggacgat tcattgcaat catccttatt
     8041 gtcatgctag gaacactttt atttgtaggt gtcaaaactg ctggacctgt catgcaaaaa
     8101 acgatggatc actatgttgg tacagcagga ctttctgatt tgcaaatcgt ttctactggt
     8161 ggcttgacac aaaaagatat tgctcaagca gaaaagatcc cagatgcaaa agtagaaaca
     8221 ggaaaacaga tctattattc caatactggc aaaaacgaag tgattcaaat attttcttat
     8281 aacaaaaata acaagcagaa caagctacaa ttaactgatg gaaaactccc ggaaaaacca
     8341 aaccagcttg tattggatga aaaagcggaa gatgaagggt ataaaatcgg tgatatttat
     8401 cagattgatt cagatgaatt gaaagaaaaa gagtatacca tcacgggctt tgtccgttct
     8461 ccattgttca tcaacaattt ggagagagga tacgcgaacg tcggaaatgg tagcgtcgat
     8521 tattttgtct atttacctga atccgttttt aaaacagata tttattctgt cttatatctt
     8581 gattttacaa acgtaaagaa tttgaatacg tacagcaaag cttacaaaga taaaatggag
     8641 caaaatcaag aaaaggcaga aaagtattta caagatcgac ccgaagaacg cttagaagag
     8701 ctgaaaaaat cggccaacga agaattagaa ccggcacagc aaaaaatagc ggatggcaaa
     8761 gcacaaacaa cgcaagcaag aacacggtta gaagatgcta aaaaacagtt agcgcaacag
     8821 tcagcaacta tccagcagct tcctgaagcg caacgaacaa tcgctgttca aacattatct
     8881 caacaagaag ctcaacttgc tgaacaagaa aggcaattag ttgagaaaga acgtgaactg
     8941 gcctctgcag aggaagaatt gaatcgaaat gcagaaaaaa ttgaaaattt aactaaacca
     9001 tcatacctat acaatgaacg ttcagaaaat gtcggttttc aagaatttgg tgatctgtct
     9061 gatcgtatcg ctgcgattgc caatgttttt cctgttttct ttttctttat tgccgcattg
     9121 atcaccttta cgacaatgac acgtatggta gaagaaaacc gcagggaaat cgggacatta
     9181 aaggcgttag gttatacgaa agttgaaatc gcaaaaaaat atgcaatcta tgcaagtcta
     9241 gcctcaggga taggtattgt tttaggaacg atattaggaa cgaatctttt gccaaggatc
     9301 atttttgagc tgtctaacga gcggtatgat attgggagtg cgcttatttt ttatgactgg
     9361 ccgccgattt tacaagcagc tgccgcattc ttcattgccg catttggcgc tgcaatgatc
     9421 gttttattca aagatttaag agaacggcct gctgcattgc ttcaaccgaa agcaccgaag
     9481 ccagggaaac gcatttttct tgaatacatc actccgctgt ggtcacgcct tagctttaat
     9541 caaaaaatca gttaccgaaa tctcttccga tataagtcaa gaatgcttat ggcgattatc
     9601 ggaattgctg gatgtgccgg gctgatggtt gcaggcgtgg gattgaaaga ttctctaagt
     9661 tctgtttcag ccaaacaatt tggaccgatc attgactatc aagcaatcgt tacattggat
     9721 tcatccacgg aagatagtcg tcaaaaagca gcggatatgc tgcagaagca agaaaaagta
     9781 aatgagatcc tagctattca tactcaaacc gtagaattga gaaaaaaagg acatgcttcg
     9841 caaagtctga cagtgatcgt accaagtaaa acagctgatt tggatcgttt tatccattta
     9901 actgatcttg aagagaaccc acgctccttg cctaaaacag gaatcgctat tacgcaaaaa
     9961 gtagcagaac tatttgactt atctacagga gatcatattt cgctttatga tgaagatcaa
    10021 aaagaatgga aagtcgaagt aaaagaaatc gtgaaaaact atctaggtag ctttatttat
    10081 atgtcccctg aatattatgc ttcagttact gatcaatcga tgatggataa tgcatttttg
    10141 ctgcgtacag ataagatgac aacaaaagaa gaagaaatgc tttccgagaa actgcttgcc
    10201 acagacgctg tgacaaatac gtctttcatg tccaaacaaa tcgaaacgca agaagaatca
    10261 atggcgaact tagactcggt tgtcttaatc tttgtcgtat tatcaggact gctggctttt
    10321 atcgtcttgt ataatctaac taatatcaat atttccgagc gtgtacgcga attatctaca
    10381 ataaaagtcc ttggattttt tgataaagaa gtcacgatgt acattgttcg agaaaatatc
    10441 atctttactc ttttaggaat catcggagga tttggtgtag gatatatatt gactgacttc
    10501 atcttgcaac aagcgtctat ggagaatgtc atctttccat tagtgatcac atggtgggct
    10561 tatgtcttat cagcaggatt aactatcgta tttacggtta ttgtgatgat tgttacccat
    10621 ttcaaattaa agcatatcga tatgattgat gcgttgaaat cgaatgaata aaaacagagg
    10681 ttgtgaaatc agcggtttga cctgagtatt agagaaacaa acggcaaaat agtttttcat
    10741 attttgccgt tttttgagct aataccgaag gacagctgat tgaacaccat gaatcagagg
    10801 ttgtaaaaaa gccgtcctgc atcgagcaaa aagaatactc aaataaaatt agctcctcat
    10861 gttttacatt tgagaagact gattgccgta gataaaagct tttgaacacc atttatagaa
    10921 gaaacaagaa tattttgcta ttttaaacaa gaggatagac aaatacatgt ctgatctctt
    10981 gttttttatt gggcaacaga cctcttagaa tgtataaaga taaattttac aaacgctttc
    11041 ggttcagtta taataataaa tgataacttg tatatataag ttgtggtagg taataaatca
    11101 gttgaatgga ggtaaatatc catgacattt aaagacaaag tcatttacca aatctatccc
    11161 aaatcttaca aagacagtaa tggtgatgga attggagatt taaaaggaat caaagaaaaa
    11221 ctcccgtatc tgaatgatct aggcatcgac atgatttggc taaaccctat ttatccaagc
    11281 cctcaaaaag acaacggtta tgatatctct gattacacag ccatcgatcc tatctttggt
    11341 acgatggagg aattcgaaca gctaatagcc gaagcaaaga aataccggat agaaatcatg
    11401 ctcgatatgg tgctgaatca cgtttcgaca gaacatgaat ggttcaaaaa agcactagca
    11461 ggggaccagc attatcagga cttcttcatt ttaagagaag agccaacgga ttgggtatca
    11521 aaatttggtg gaagtgcttg gtcgccattt ggagatacgg ggaaatatta tttgcactta
    11581 tatgatcgga cacaagctga cttgaactgg cggaaccaag cagtaagaga ggaactattc
    11641 aaagttgttc gtttttggat ggaaaaaggg gtaaaaggat tccgtttcga tgttatcaat
    11701 gttatcggta aagacgaaca attgaaaaat aataaagaaa atgatggcaa acctgaatat
    11761 acggacaaac caatcaccca tacgtatcta aaagaattga accaagcaac gtttgggacg
    11821 gattccacta tcatcacagt cggtgaaatg agctctacga cgatcgaaaa ttgtatcttg
    11881 tatacagaac ctagtagaga agaattatca atggtcttta atttccatca tttgaaagtc
    11941 gattatgaaa atgggaataa atggtctact cctccatttg attttgcaga attaaagcga
    12001 ctatttcata catggggaga aaagatgagt gagggtaacg gctggaatgc tctatttttt
    12061 aataatcatg atcagccacg tgcgctcaat cgatttgtag ctatcgatca gtatcgaaaa
    12121 caaggagcag agatgctagc aaccatgatc catttgaacc gtgggacgcc ctatatttat
    12181 atgggagaag aaatcggcat gatcgaccca gactattcat cgatttccga ctatattgac
    12241 gtagaaagtt tgaatgctta taagttactc cttgaacaag gattcacaga aaaagaagcg
    12301 tttcgtcgga tcaaagcaaa atctcgcgat aactctcgaa cacccatgca atggtcagac
    12361 acagaacaag caggatttac tacagggaaa ccttggttaa aagttggagg caagctagaa
    12421 gagattaatg tagaaaaaga actatcttct gaagattcta ttttgtccta ttacaagaaa
    12481 ttgattcggc tgagaaaaac atatccaatc gttgcacaag gagattatca tgcttatgaa
    12541 gcaaatcatc cggaagttta tggctttctt cgccaatttg aaggacagca gctgcttgta
    12601 ttaacaaatt tctatgataa agatacaagg atcgccatcc cagaaggatt cacagaagcc
    12661 aatattctta tcgataatta ttccgatact gttcttcatc aaagtctgga gctaaaacca
    12721 taccaggcaa ttgctttgct gaaaaattaa aacaaaaaca caaaacgtcg ttcctacgag
    12781 ctattctcgt aagatcgacg ttttgtgttt tatttaaaat aatcttcttc agaaaatgaa
    12841 gagttttcat actctccttt atcgccataa aatttattgt agcgttgttt gaatttacgg
    12901 aaaataaaag taacgagtaa tttgactaac gtatagatag gaataccaaa aataacgccc
    12961 acaattccca ttagatcacc catgacaagt aaaacgatta agatcgtaat cggatggatc
    13021 tgtaaacgat cacccattac tcgaggaatc accaagtcac cgtggagaag ttgtacggca
    13081 aaccagacaa tgacaaattt gacagccata aaagtagagt cttggaatgc aatgatcaaa
    13141 cctggaaaga aggcgataaa cggcccaata tatggaatga tacataacaa cccagcagaa
    13201 atagctaaaa cactagcaaa ttctaatccg atcagtaaga aacaaaccca gtagactgct
    13261 ccaaggataa atgaagcaat gatttgcccc tttagaaatg aacccaattg ttgattagca
    13321 atctttgtca aatttttaaa atctgtacga aatgctggtg ggacaatcgc taaaactgac
    13381 tgataaaatt tttgaggatc tctcaaaagg aaaaaagcga caattggacc agtaaataaa
    13441 gtaataaaaa tagctgtaac agttgaaaaa atgttgctga tccgttgtgc gccactttcc
    13501 caataatttc caataaatcc agctatatca gacgtcacgt cgtcaataga agcgaaaaat
    13561 tgattaaatg aatcggcaaa aggtgttttc tccaaaaatt gtcggagact ctcttgaaaa
    13621 tcttgaaaca aatctggcaa actactgatc aatgtctttg cctgttcttc aagaaaagga
    13681 ataagccata tgcttccaag ccccaataaa aataaaataa tcacataaac gataatgact
    13741 gacaagttcc tcgaaaggtg cttgctcaaa cgtttgatga ctggttccaa aagataatac
    13801 aagatcagtg caaacaataa tgggggcaag atagtagcga taatgattcc gacaggcttg
    13861 aatataaacg agatctgatg gaaaaaataa atgatcagcg caagtaagat aactattcct
    13921 aaaacatagt aagaggtttt tcctcctaaa aagcggatca aacgattcgt tgtatctgtt
    13981 tcttttttca ctctttttcc tcctttatct ataacttcat tatagacaaa agcgggtata
    14041 aaaggaaaat gatagaggtt gattgcaaaa atatcgtcaa tcattcatat aaactttctc
    14101 atcaattaaa ataagaaaac gcttgacagt tcttttaacc atatgataaa ttaaatcctg
    14161 ttgctaaaac gtaactatta cggtttgcga aaagcgaatc ggaacattca aatgaaagaa
    14221 gatgagaaga ggagtgtcat aaatgaaacg attgataggt atattggcca tgctggtttt
    14281 agcaggtatg ttaactgctt gcggggcaag tgggaaagcc gaggacagta aagaaaaatt
    14341 gagcgtgatg acgacctttt atcccatgta tgattttaca aaagcaatcg tcggggatga
    14401 aggagaagta gagttattga ttccagcagg gactgattcc cacgattatg aaccttctgc
    14461 taaagacatg gcaaagatcc aagatacaga tatctttgtt tataatgatg aaaacatgga
    14521 aacatgggtc cccgcaatcc aaaaaacatt acaagaaggc aatgtccata cgataaaagc
    14581 gacagaaggt atgcttttac ttccgggaag tgaagaagga catgatcatg accatgaaca
    14641 cggggaagaa ggccatacac atgagctaga cccacatgta tggctagctc caagtttagc
    14701 aatcaagcag gtagcaaaca tacgtgatca gctgattgaa gcttacccag aaaaacagga
    14761 agtatggaca aaaaatgcag cagcctatac cgagaaactt caagcactcc atcagcttta
    14821 tcaagaaacc tttaagcaag caaaacaaag atcttttgtc acgcaacatg ctgcattcaa
    14881 ctatctagcg ttagaatatg gattgaatca agtatcgatt gctggtcttt cttctagtga
    14941 agaaccgtct gcggcgagaa ttgctgaact gaaacacttt gttaaagaac atggaatcaa
    15001 ctatatttat tttgaagaaa acgccaaaga tagtattgcc agaacgttag caaacgaagc
    15061 aggtgtatct ttagaagtat tgaatccttt ggaaggattg acgaatgaac aaatagaaaa
    15121 tggagaaaac tatctttcta tcatggaagc aaacctcgaa gcgttgaaaa aaacgacaga
    15181 gacagaaaac ccgctagaag atactttgac ccccaaaaaa gaaaagagcg tctacaacgg
    15241 ttattttgaa gacgaggatg tacaagatcg ctctctttca gattgggcag gaacttggca
    15301 aacagtagat acgtatgtag aagatgggac ttttgatcct gtttttgaat ataaagaaaa
    15361 attgaatcag gataagacag cacaagaata taaagaatat tatacgaagg gctatcaaac
    15421 agatattgct tcaatcaaga tcagtgagaa agagatggca tttacgttta agaatggaac
    15481 aacgaaacgt tcaaattatc gttatgtagg gaaagaaatc ttgacctacg aggcaggaaa
    15541 ccgtggcgta aggtatctat tcgaagcaga agataaggaa agtggcgctt ttagatatat
    15601 tcaattcagt gatcacacga ttgcaccggc tgactccgaa cattttcatt tatatgcagg
    15661 aaatgaaagt caagaagtat tgctgaaaga aatggagaat tggccaacat tttatccaga
    15721 tacaatggat ggacgtgaaa ttgcacagga aatgcttttg cactgatagg caaaaaagaa
    15781 ccgaagggtt tggatcggct ggtcatttag tcggtccaaa ctattcggtt ctttttatga
    15841 aatcaatttc tttaatccac ggatagaggc tgggatggtc tcgccgtttg ccaacgtgac
    15901 ttctctggtt tttttattaa tcgaccgcac attttgtgta ttgataacat aggatttatg
    15961 acaacggata aaattccgat gcattttctc gatttctttg atcttctcat agaattgtaa
    16021 ttgggcatga gtcaaatgga cttccagttt atgtggcgct cctagcgaag tagaaaaata
    16081 ttggatgtct ttgatcggta aaaatttgat ctcatcatta aactctattt tgaagatatc
    16141 tttttggatt tgggacattt gaaaatcttc gaaaattttg ctcaggatat gctcgacatt
    16201 ttcttttaaa tcataaagat cttctttgac gatataatca gtcggttcga tgttgctttt
    16261 taagattttc atcgacattt ccgtatggga agtcacatag attatttttc ctaaaggatc
    16321 taattcacgg atcaatttac cgatcgctac accatccatt tcactgtctt ctagttcgat
    16381 atctaaaaag taaataccaa aagaagaagt tctgatcttg gctgcattga gaagttcttc
    16441 cggatcagag gtagaaatat ggacataact gtctaagttt tcaatcatga ttctattttt
    16501 gatcaccgtt gataaaactt ctaattgttt tggatcgtct tcacaaatat aaatgggaat
    16561 catatcatta ctcctttatt ttatttgtat ataaatcgtt tgagaaaaac gctggttttc
    16621 aattgatgtt tcaaggaaaa gataaggatt ggtctttgtc aattcatcca agataaacaa
    16681 gccaaggcct tcgttttttg gatttgtttt tgttgagaac cctttttgtt tcaactggtt
    16741 taacggtaat gtatttgttg ttgtattgat cacacgaatc atcacggaat gattcttctt
    16801 gagtatggtc aattcgattt ttttcttctt tgcagcacta gctccttcaa tcgcattgtc
    16861 caagataata ccaagcatac gtacaaggtt taccgtatgt ttcgtatcca gttcgattgg
    16921 ctcaggaaaa tgaagctgga cgatcaaccc tttttcctgt gccatcatga gttttaatga
    16981 cagtaaacta cggatctcag gaactttcat attatccaac atacttagat tggccggaaa
    17041 aaggttcatc atctggcgtg taggtaatat cgtttgatca taataagacc gtaatccttc
    17101 taagtcgcct tctgaaatat agctgtgaag agaaaaaagc agattttggt aatcatgttt
    17161 gaattctctc aagtcttgat agtttttttc tagatctttg atataatcct gttgcatcct
    17221 gacttgttgc tctaataact gatcagctaa tttcttatta gagattttcc aataataaac
    17281 caagatgatc aggccaacac acagatatag aatgaaaaaa ctcgtattca aagctaagac
    17341 atcaggtgtt tcaccagaaa aacgggtata aaaaataatg atatagtatg taaataatat
    17401 caatgtcccg atcgtaccga tcatacggcg gtccacagtg tgaaaccaag tctctaacga
    17461 tttttttaaa caaaaagcta ataaagcaga taaaaaagcg gataagacaa tgtgaaaata
    17521 ctgcaatgaa tctcctggtt caataaccga agatttcaaa acaaaaaaat cgccgatgct
    17581 tgttaagtga tctcctaaaa gcgacgtcaa taacccaaaa ggaagcgcgt aaaccaattg
    17641 ttgacgcgaa gtcgaaaaga agaaaaaagt aagcatcagc attcctatag caattagagc
    17701 agtaaaaatc cctaaaatcg aatagaccca tccaatcaaa atacaagaaa tcagaatgac
    17761 tcccgcaaac agatatttgt atcggatgtg atggtcaatc aataagatac aaattgtgta
    17821 aatcaaatat tgcaacgcta tggaccataa aaacataaag tacagctccc attgattttt
    17881 accaaattat agcacaattg gcgtattatt tttttaattt ttcagggatt ttgaattcag
    17941 aactcatacc aaagcagcag ccagatacaa aaaagtcaga taagttagtt aattgtttaa
    18001 tgattgtttt tttcatgaaa gattcctcca aaattcatca agtagtttgt aataggaagc
    18061 aacgtagcta tttctaaaac aatcccaagt agcaaatagg tagaaaagta agaagctgag
    18121 aaaaataacg taatgagaga aaaaattaga aacatgctga tcgccttctt taataatggg
    18181 ggacgtttct cgggttcaat gggattgatt gctgtacctc ttgatccata gcgaagcagt
    18241 aacaaaaagc agctgattaa caaaaagacc atcattggga aggagatagt caattgcaga
    18301 tgtttcatca ccaagggaac gccccataaa taaagtagtg tccacagcag acaaacgaat
    18361 tcagagtcgg aatgcaatcc ataagcatat ctgcggatgc agatataaga aagaaaggtg
    18421 attcctgttt ccgcccatga gcctgtcacc agagaaaaga ctagaacgac cgctagtttt
    18481 ccaagattat tcaataaaac ttctaagaca aattttactt ggacatacac catatcttct
    18541 tccgatttgt ctccgctgaa taaggcttct aataatttgt tcacaacttt atctttcatc
    18601 tcatacgtac ctccaccaca aattttatgc taaaaaaaat aaaaaacgtc ttttgtttca
    18661 taaaaagtgg ttttgacatc aaaaccgtca tgatctccta aaaaaatctt cgttttccaa
    18721 gaaccttacc aaaaaaagtg acagttttga tacgaaaagt actttttatg acaagtttgt
    18781 gctttttcga gtgatttatg ttaaaaaatt actgacctta taaaaggtga tggagggatt
    18841 cttatggaaa atgaacaatt gtttgaagtc ggcgaaacat atgcatttaa atctgattta
    18901 tttgaatcac caataaaagg aaaaatagaa aaaatctatg aaaatagtgt gatgatacgt
    18961 gtcatgaaat gcgcagtaga agacaatgat accgctcata atctgcactg gcagttagtt
    19021 gtacgcaaat caagtacaat agaagaataa aaacagataa aatgttgaga gcataaagag
    19081 gttgtgagtc tcccgtttag ctctgagtta caaggaataa tgttaaaaaa cagcttttca
    19141 tgttttatga atattgcctt gtgaccgagg tgttggcagg agaatactgt ttatttgaaa
    19201 aaagggctgc gacaagactt ttgtcacagc ctttattttc atgaatagga gttacttttg
    19261 gctactttta gagaagtagc ttttaaggag cagataaagt caaggctttt atttctataa
    19321 atgaagtagt aaaatgaaga acagatttta ttacaggaag gatgccgata attgtgatca
    19381 ttgttgcagg aacgatcggt gcagggaaaa gcacactaac tgaaatgctt gcccaagatt
    19441 tagaaacaaa acctttttat gagaacgtgg aagataatga agtattgccg ctattttatt
    19501 ctaatccaga aaagtatact tttttattac agattttctt tttgaacaaa cgctttttgg
    19561 caatcaaaga tgctttcagc catgatgaca acgtactaga ccgctccatc tacgaagata
    19621 gtatgctgtt tcatttgaat gcagatttag ggcgtgtttc tgaagtagaa gtaaagcagt
    19681 atgaaggtct tttagaaacg atgctgaagg agttagaaga aatttcgcca caaaagaaac
    19741 cagatctact ggtttatatt cgtgtatcct ttgaaactat gttagcgaga atcaaaaaaa
    19801 gaggccggga atatgaacag ctagaacaag atcctgaact ctattcctac tataaagaac
    19861 tcaatcgacg atacgaagag tggtatgaac agtttgatat atgtcccaaa atagtgatcg
    19921 atggggacaa atatgatttc gtagccgatc cggcatgtgg agaacaaatc gtacaagaaa
    19981 tcaagatgcg ggcaaagaaa atgacagaag aggcagatgc ccagtcgatc cgctacacta
    20041 caaataaccc gaaagcaaag ggaatcttta caactaaata atataaaaac taagcaaatt
    20101 gctcactcgc agatatttct ctgtgagcag catcttgctt agtttttttg attcattgag
    20161 atcaaatagt gaaccgattc gtatgataaa attgccataa atgaaatgtc tgagtgaggg
    20221 ccatgatcaa aaataaaatg gaaacgatca aaatcgaatg ggcgccaaac agatgatgac
    20281 agaaaacaag gacaatagct cctgcgataa aggcaagaac gacacttaga tagtggaaaa
    20341 atcgatcaaa atctgctttt tctttagaaa tacaataatc atataagttc gttgctaccc
    20401 cttttaaatt tccagttgta aataaattag tgtaggcacg cccgttgatt ttatcaaaag
    20461 ctacccactg gatcgatgca gtaaaagcaa tcaaaatcgt tactaatgcg ggatgctcat
    20521 ggacgggtgt caaactaaca aagaaaaaaa tcactgcttc ataatataga acaaacaagc
    20581 gccagaaatg tttttctttt tttctaaaat aatcaattag atactttgtt agaagaatcc
    20641 ctaagaaaaa gaaaagggta gacaatactt ttttaccaac acttagccat tctccgtcaa
    20701 atgcttggat gatcgataga atgatattgc cagtttgagc agaggcaaaa gcaccatatt
    20761 ggatataagt ataactgtcc atggctcctc cgataaatgt cagcaaaagt cctattgtac
    20821 gcccctcgtg aaaggaagtc tgcatttttt tcatcacctt ttatgaatat ttttgtcttc
    20881 cttatcttag cataaacgat tgaaaaaaaa ggaaaaattt tcagaaaact aaaatcatat
    20941 cgcttcccat tttcattttt agaaaggtct gttagtataa agaaaaaata gacaacgaaa
    21001 ctttaaataa ccaacttgaa ataaacaaat tcaaagaaga aagatgggaa ggaaatcaat
    21061 cagttgaatg aaaaagaaat tctccgatta caaaaagaat tgaaacgctg ggagaacaag
    21121 tataaaaagc ttcaacaagt ctttcggcta caagaagaaa tcattgctac ctatgaagcg
    21181 gcttatggcg aagcacccgg acttattgag gaagaagtac aagtaaaaga aagtgaagga
    21241 aacagctctt tctttaaaat gatggagaaa cgttcctata atcgcttcaa tcaagaacaa
    21301 aaagcagcta tcacatatga tatgaagaaa catcttcgaa tcatagcagg agctgggagc
    21361 ggcaaaacgc aaacaatctg tgcaaaagct gtttatctga tgacgcaaaa acaagtagac
    21421 gaagaacgca tactaatgat cacttttaca aggaacgctg caaatgaact aaaaaaacga
    21481 gtagataatt tcagccagag aaaaaccgct gtccatatcg gaacattcca tagtatcttt
    21541 tttaggatat acaatgaaat ttgtcgaaaa tttcctgaag tagcaatgca aggaatacaa
    21601 ggagactttt caaaggatac tgcacaaaaa gtgaatgctg ttttgcaaca gctgatacgt
    21661 aaatataatc tttatatatt tgatcaatat ggagaaaaaa cgattgcttc tcggttagac
    21721 tattggcaga atatgaacct ttcagtagaa gaaatggttc agttagttaa agaaaaatat
    21781 gattccattg ataagaatgc acgacagccg atatcagaac gactgtatgg attgctcacg
    21841 gaactccaag aacaaaaaag aagccagcaa ttgctagagt tcaatgatgt actccaaaat
    21901 ctgaaaagcg ctttggagaa tgaggagatc cagcattata tcggccaaaa atatgattat
    21961 ctattcattg atgaatttca agatactaat ccacttcaat gggaaatcgt gaaacggatg
    22021 acaaaggaaa acaggatcaa gttgattgtt gttggagatg atgatcagag tatctatgga
    22081 ttcagaggat cagagccagc atacatcaag aactttgaaa aagaatatcc taccaaaact
    22141 cttttcttat taactaatta tcgttctcgg gcagctattg tccaaggagc aaatagattg
    22201 atcagctata ataaaaatga tcggatacca aaggcgatga ttccagcaca aaaagaagca
    22261 ggtgttatgg aagcctattt gttttctgat actaaagcag aaagtcagtg gctaatcaaa
    22321 caaattcaga catttattca aaaaaatggg aaatataagg aatcaatcat attataccga
    22381 tcagcttcac aaacgcagca gcttgtccaa tctctgctga aaacgaatat tccttttgta
    22441 ctggaagcag atagtccata tgaaggtatc tttggtatca agtctttcca acatttctat
    22501 caaaaaattg ttcattggca aacagcactg aatctgcaaa agaaaaatca ggcctaccag
    22561 caattgctcc ggcagctgat ggcagactgc tacctgaaaa aaagtgaatg cgatcaatac
    22621 ttttctccta aatatcaaaa tatagccgtt gcagattata ttttgtcgat aagccccaat
    22681 ttaaaatcga aatctgcaga aatccgagag tttgaggaag cgttgcgtaa aacagaccag
    22741 catcaagaaa tctattcgct tgttcaaagc tatcttcatc tgccacgtat agctaaggaa
    22801 atcgaccaga gtgatcaaga atggatacga gaagaagtaa agcagcatgc tactttcaga
    22861 agactccaag ccttatcgca agaaacccaa gcgacagcta aagaattaaa aacccgcttg
    22921 attgcttaca gacaaggaaa acttgatgca ctatgtatcc agagcattca caaaagtaaa
    22981 ggtcttagtt accgaaatgt ctttttgatt ggttgtaacg aaggaagttt gccttataac
    23041 ggagcgacag aaaagaaagt aatcgacttt aatgaaataa aagcagaacc agtaacgacg
    23101 atagaagaag aacgaagact attttatgtt gcgatgacta gagcaagaaa tcgtctgtat
    23161 ctatgtgtac ctcaaatgaa aaattcaaga aaattgaaag tatcgagatt tatcaaagaa
    23221 accgggagtc gtgtgaaaaa aggaaagtga tagaagagag ggtgtgacag aagtggtcag
    23281 tttcaagaaa gaagaaggaa tgtacgaaaa ttgcttctca aatttttgtg aaatttcagc
    23341 ttattttcga agaagctatt tctggaacac agtttattcg gaaaagggag gtgtgacaag
    23401 aattttgtca cacctccctt attt
//
LOCUS       DAJSKS010000032        23238 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJSKS010000032 DAJSKS010000000
VERSION     DAJSKS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.033"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..666
                     /locus_tag="ON396_001078"
     CDS             <1..666
                     /locus_tag="ON396_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3113179.1"
                     /translation="ILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISY
                     FVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASV
                     MGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPR
                     NSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDS
                     ALF"
     gene            678..1598
                     /locus_tag="ON396_001079"
     CDS             678..1598
                     /locus_tag="ON396_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3113180.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1639..3096
                     /locus_tag="ON396_001080"
     CDS             1639..3096
                     /locus_tag="ON396_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3113181.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3441..4055
                     /locus_tag="ON396_001081"
     CDS             3441..4055
                     /locus_tag="ON396_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR3113182.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4048..5772
                     /locus_tag="ON396_001082"
     CDS             4048..5772
                     /locus_tag="ON396_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3113183.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5769..7220
                     /locus_tag="ON396_001083"
     CDS             5769..7220
                     /locus_tag="ON396_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3113184.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7693..9846)
                     /locus_tag="ON396_001084"
     CDS             complement(7693..9846)
                     /locus_tag="ON396_001084"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HCR3113185.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9848..10894)
                     /locus_tag="ON396_001085"
     CDS             complement(9848..10894)
                     /locus_tag="ON396_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3113186.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11044..12333
                     /locus_tag="ON396_001086"
     CDS             11044..12333
                     /locus_tag="ON396_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HCR3113187.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12346..15048
                     /locus_tag="ON396_001087"
     CDS             12346..15048
                     /locus_tag="ON396_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR3113188.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            15033..15890
                     /locus_tag="ON396_001088"
     CDS             15033..15890
                     /locus_tag="ON396_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3113189.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(15961..16860)
                     /locus_tag="ON396_001089"
     CDS             complement(15961..16860)
                     /locus_tag="ON396_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR3113190.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            17029..19200
                     /locus_tag="ON396_001090"
     CDS             17029..19200
                     /locus_tag="ON396_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCR3113191.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            19268..19609
                     /locus_tag="ON396_001091"
     CDS             19268..19609
                     /locus_tag="ON396_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR3113192.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(19885..21639)
                     /locus_tag="ON396_001092"
     CDS             complement(19885..21639)
                     /locus_tag="ON396_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3113193.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLSFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(21650..22486)
                     /locus_tag="ON396_001093"
     CDS             complement(21650..22486)
                     /locus_tag="ON396_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR3113194.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(22488..23171)
                     /locus_tag="ON396_001094"
     CDS             complement(22488..23171)
                     /locus_tag="ON396_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HCR3113195.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT         7637 a         4157 c         4781 g         6663 t
ORIGIN      
        1 atccttttga atctgctgat ctcagtcgta tttgcaatca ttatgagcga gttgagaaat
       61 aaacgactcg ttaaagttta tcaaacgatg tccttactac cttattttct atcttgggtc
      121 attatcagtt actttgttta cgctttttta agtcctgaca aaggaatatt caatcagtgg
      181 atcactggac atggaggaga agctgtcaat tggtataatg aaccgaaata ttggccgttt
      241 atcttggtgt tcattggtac atggaaaggg attggttata acagcattat ttactttgca
      301 tctgttatgg gaatcgatcc gacttattat gaagctgcta tggttgatgg agctagcaaa
      361 tggcagcaga tcaaacatgt gacgatacca caacttattc cactaatgac gattttaacg
      421 attttagccg taggaaacat tttccgagct gattttggcc tgttttataa cattcctagg
      481 aattccggtg cattatatga tgtgactcaa gttttggata cgtatatcta caatggcctg
      541 acatccacag gagattttgg tatgacggct gctgcaggac tttaccaatc agttgtcggc
      601 tttgtcttgt tgatgatcac aaatacgatc gcccgacgtt ttgatagcga ttctgcatta
      661 ttctaaggag gaaaaaaatg aaaaaaaaga aagtggagag agtccaagtc cgctctttca
      721 ataagacggc taattttttc tctaatataa tgattgcact ctttgctatt tcctgtattt
      781 tgccatttat atttgttatc gccatctctt ttaccgatga gtccatattg tcgactcacg
      841 ggtacagttt ttggccagaa aagttcagta tatttggtta tacgtacttg ttcgaccaga
      901 tgcaggataa aattttccaa gcgcttttcg tcactgtact tgttactgtt ttaggtacac
      961 tgatcaatag cacggctact tctttatatg cttatgcgat ttcacggtca aactttccat
     1021 tccgtcgttt ttttaccgtt ttttgtttga ttacgatgtt gttctcacca gggatggtgg
     1081 ccaattatct tgttatgaca aatcttcttc agttaaaaga tacgatttgg gcattgatct
     1141 tgccaatggc tgtcagtccc ttcaatatta ttgtgatgcg cacattcttt aaacgttcgg
     1201 tctctgattc gattattgaa tcggcacgga tcgatggagc tagcgaactg cgaatatttg
     1261 ttcagatcgt tttaccgtta gctgttccag gtattgcgac aatcagttta tttgccgcat
     1321 tgggttactg gaatgactgg ttcaatgctt tactctacat ccaagatgat aagctagttc
     1381 cactacagta tcttttgatg aaaatccaaa gtaatattca atatctgaca caaaattcag
     1441 gagcaggaag ccaattgaca ggcggattag cctcagttcc aggagaatct gccagaatgg
     1501 cgatcgtagt catctcgacg ttgcctattg cgatcagcta tccatttttc cagaagcatt
     1561 ttgtcaaagg tctaacaatc ggcggagtaa aagaataaag aaacaaaaat ataaatcata
     1621 aaattaggag gaagaaaaat gaagttatgg aagaaactag cctttacagg tgtttcagcg
     1681 ttgttgttgg gaacattagc agcatgcgga ggttctggtt ctaaagacca ggcagaagct
     1741 agcaattcgg acactttgca gatgtatcaa atcggagata aaccagataa tttcgatcaa
     1801 ttgatggaag tagcaaacaa acgaatcgaa aaagagatcg gtgtaaaagt caatatcaat
     1861 tacattggct ggggcgatta cgaaaagaaa atgaacgtca tcatttcttc cggggaaaat
     1921 tatgatatcg cctttgcgaa caactatgtt tccaatgccc aaaaaggagc ctttgctgat
     1981 ttgactgaat tagcgcctaa gtacgctgaa aaagcttata atgatttaga tgaagcatat
     2041 attaaaggaa atctggtcaa tggaaaatta tatgctttcc cagtcaatgg aaatgtcttt
     2101 gctcaacaag tacttacttt taacaaagct ttactagata aatacgatct ttcaattgat
     2161 ggaatccaat cttacgctga tgcagaaaaa gtcttgaagg aattccatga aaaagaacca
     2221 aacacggcag cttttgccat tggacaaggc ttcaaagtcc aagggaactt tgattatcct
     2281 ttagggaata cgttaccatt tgctattgat ctggatggag atgattcgaa aatcatcaac
     2341 caatacgata atgaaacgta tatcggactg ttacgtacaa tgcatagctg gtacaaacaa
     2401 ggactcattc caagtgacgc tgcaacgagc aataaggatt atccactaga aggaaatact
     2461 tggctaatgc gtggagaaac acaaggaccg tatgattatg gcgatacaat tttgacaaat
     2521 gcagcaaaac aagaacttgt ctcaaaagca attactgttc cactaaaatc tacagctcaa
     2581 gcacaaatgg caaattttgt cgtatcaaat acttcaaaaa ataaagaaaa atcagtagaa
     2641 cttctagggc tgcttaacag tgatccagaa ttattgaatg ggcttgtttg ggggatcgag
     2701 ggagaagcat gggaaaaagt cgatggttca gatgataaaa tcaaattgct agatggttat
     2761 cagccaaata cacatatgtc tgcttggaat acaggaaaca acaagattct atatacacaa
     2821 gagtctatta cggatgacat gattgccaaa cgtgatcaat cgattgctga tgcagaaact
     2881 tcaccgattt taggattcag cttcaacact gacagtgtca aaacagaatt aagtaatatt
     2941 tctaatgtca tgaatcaata tttagatgga ttgaatacag ggactgttga tccagatgaa
     3001 acattaccga aattgaaaga cgctttggac aaggctggct atgataaggt attgaaagaa
     3061 atgcaaaaac aatatgatga gttccgacaa gaataataat gaataaagac aaaggggttg
     3121 tgacaaaaga cgtgtcacaa cctcttgttc cgaataaacg gtgttcaaaa gccagtttct
     3181 cggtattaag ctaaatatat gtaaaaatgt ggaaagccat attcacaaaa tttttcttac
     3241 tactcgaaac taagcggcta tttcacaaac tcttggtaaa cggtgttcaa tcagctgacc
     3301 ctcggtattc gctcacaaaa cgacaaaata tgaggaacta tttttccgtt tgtttctcta
     3361 ataatcaggt caaggtgctg attgcccaac ctttttttgt ataatgagaa agtataaact
     3421 taatgtttgg aggagaagat atgttaggga aggttttgga agagttattt atccggatct
     3481 gggtcattat aaaactaacc ttgtatttct ggatatatac atttgccggc gggatcatct
     3541 ttggattagg tgcggcatgg aaaacagtca atgaactatt ttatttgtac ggattcgagt
     3601 ataaagaaat cacaataaaa agaggctgga atatatataa aagaaatttt ttaagaggca
     3661 atctgctatt tagtttattt ttatctggta ctgctttatt gagttacaat ttatatttat
     3721 ctgttcaaat caaagggctc atttttttag ctattgattt tattttactt tttgccttgc
     3781 tctgcctatt tgctacttat caatacagta tgattttaga tagtgaatat acgatctctg
     3841 ttcctaatct gctaaagcta agttttattt cggttttttc cagcttttcg tcttttctaa
     3901 aaataattat tggtagcggt ataattttag gcgtgacttg gcaattcaaa ggattgatcc
     3961 tgtttggtgt cattggtctt ttgacggttt ggaatggtac gatgacaaca cattggcgtg
     4021 aagaattaga taaacagttg gaaagttatg aataagtttc ggattctatt ttcaaaagga
     4081 accttactaa atcgtctgtt gcggaaatac gcggttatca tgatcggtgt aacgatgact
     4141 gctaccgtta tttttagtat ccatacttgg gaacaaaatc aaaaacaggc agaaaacatg
     4201 acaagtgatg ccgttcaaag cacaagcaga atgctgaatg ataaaacaac cttgagtagg
     4261 atcatcaaaa accagcttgt agggaattcc gaaaagatcg aaaatgtgac gacctactta
     4321 acaaaaccca tagatcagta tctgatgtat gtatacgaac agcaaaacag taccgatgaa
     4381 ttagtttctt tcccaaatca aatcaaagat ctctacgcaa attatgaaga actaagtgcg
     4441 atatatatcg tgttgaatca actaccggag tattatgaat cttctagaga aaacaaaggt
     4501 ggggaaataa aaaaggggac accaaaatta ctagacgcct tttatatcaa gttgcctatc
     4561 acacaaggag gagctgagtc aggaagtatt tttattggtt ttcaaaagaa agaactagat
     4621 atgattttag aaaatctaac gtcattcaat ggactctctt tatacatgat ttctggaacg
     4681 acaaatcgac tatacacatt ccatgataat aaaatagcta aagaaacgtt tgaaagacaa
     4741 gaaatgatca tcacagagag tctgaagaaa aattcacaat tgccgattga cgatctggaa
     4801 aaaaacaatt ttgttcaaca ccaagaactg tctgatgatt atcgtatatt agctgtacta
     4861 gataaaaaaa gtgtaaaaat cgaaacggga aaaaatcttg ccccgctgat cattggtatc
     4921 attattttag acaatatact gcttatcttt ctttataaaa cattcaaacg atattcacag
     4981 caagtatcta tcgtcatgca ggccatggat caaacagcag cagggaattt agagacgcgg
     5041 atcgacataa caaacacgga atatgaactg aaggaacttt caatcggtat caacgaaatg
     5101 ttggatagca tcaatcagtt tgtagaagac atctacaaat tagaaatcaa acagcaagac
     5161 gcacatatgc gggcgcttca agcacagatc aatccacact ttttatataa tacattagaa
     5221 tatatccgaa tgtatgcaat cagcgaagga agtgaagaat tagcagatgt tgtgtatgcg
     5281 ttttccgctt tgcttcgaaa caatacgaat caagagaaaa cgatcacttt aaaagaagaa
     5341 ctggactttt gcgaaaaata tgtctatttg tatcaaatgc gttatcctaa tcgagtagcc
     5401 tatcatttta tgatcgatcc tgatttggaa aagatcgaag tacctaaatt tgtgatccag
     5461 ccattagttg aaaattattt taaacacggt attgatttta ctcgattcga taatgcgcta
     5521 agtgtcaaag tcttgcagga aggcaaacgg gtaaggatca tcatcaaaga taatggaaaa
     5581 gggatgactg aaaaacggct aaaacagatt gaagaaaaac tgtctcatcc taaagtggaa
     5641 cttcacggtt ctatcggtct gcaaaacgtg aatgaacgtt tacgtgccag cttcggcagc
     5701 agttattata tgtcgcttga gaataatgag acaggtggac tgaccgtttc tattacattc
     5761 aaggagggat gagcgtgtat caagtgttat tggtagatga tgaatacatg attttgaatg
     5821 ggttaaaaaa gataattgat tggcaaagtt taggctttca aatagtagca acagcagaga
     5881 atgccaaaga aggactagct gtacttgaac agcgacagat tgatttagta gtaacggatg
     5941 tgacaatgcc agaaataaat ggactggaat ttattgaagc ggctcaaaaa gaacgtcata
     6001 attttgaatt tatgatttta tcaggatacc aagaattcga ttatttgaaa ggaggtatgc
     6061 aattaggcgc cgtgaattat ttgatgaaac ccgtgaataa gttcgaattg gttgaaagcc
     6121 tcaagaaaat caaaacacgg ttagatcagc aaaatgaaca aaaaaatcaa caagaaatct
     6181 atcaagaact gttattcagt caatggttga atgaagaatt ggatgaaata agtgaagaag
     6241 aaataatagg acaaataagt gagaagcagc ataggatagt attgattcaa ctggcaagaa
     6301 ctcatgatac tcttatcaat agatggttgc aagaacggca acaaatcttt tactatcaac
     6361 gaaattacgg agaccgtgta ttgatcacgt tgttgttgga ggcagatgaa gttgatggct
     6421 tctgttgttt tgttcaagaa aactttcctg atcaggaatg gctgatcagt atcggggaag
     6481 agacagattc tttggacaaa atccctgaaa cattccaaca agcgaaagac aatctacagt
     6541 tgcatcaatt ttatggagat aaagacgagc atgtttttta cgcagtacaa gcagaaacaa
     6601 aagatcaaac catcgatttt tcaagtttca gacgcgtgct gcaaaataaa caattggaag
     6661 tcgctcaaaa aatgatcact gatttttttg aacagtttca gttagcagcc atgatgccgg
     6721 aagatatacg atattccagt tttttactgt ttatggagat tcaacgggaa ttgattgatc
     6781 tggaagatga ggaatattta caaggaatcg aaaaaatcca tcaggcaaaa acggtgcaag
     6841 aacttcagca gctacttctg tcattcatca aaaagcatca gagacagaaa aagtacagtg
     6901 acaatgtgga aaaagtaatc gtaatcctgc accagcatta tcaagaacca cttacactca
     6961 aagaagtgtc agaaaacctg catttaaatg tcatgtatct aggacagctg ttcaaaaaag
     7021 aaacgaaaaa aagtttttca gcttatttga accatttacg tatggaaaaa gcgaaacaac
     7081 tgcttttgca cagtaatcaa aacatcaatg agatagcaag tgagattggc tacaacaaca
     7141 ccacgtattt ctctaaatta ttcaagaaaa tcgtcggcag atcccctaaa gagtaccgag
     7201 aaaacagtgg agaatcataa aaaaaaaagc caatgtaatg aaataactta taaaaaattg
     7261 tctagaaatc taattttggg ctgttcttat tacttgaggc aaggcagctt tttcatgacc
     7321 tctcgataaa cggagttcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat
     7381 gtggaaagcc attttcacaa aatttttctt aatactcgaa actaaacagc tttttcacga
     7441 cctctccact aaatggtgtt caatcagctg accctcggta ttagctcaaa aaatggcaaa
     7501 atatgaggaa ctattttgcc atttgtctct ctaatactca ggtcagaacg ctgatttcac
     7561 aacctctcaa taaacggtgt tcaaaagctt gcatctgcgg taataagacc aaccaaacga
     7621 aaaatatgag gatctatttt tgcttggctg ttcttattac ttgatgcagg acagcttttt
     7681 cacaacctct tgttaattat agtttgataa ggaaaaaggc aaatcttttg cttgatattt
     7741 cttagggtga gggatgatcc ctaattggta agagatcgtg ccgcctttca gcaaatcttg
     7801 atgggtaaaa tataatttcg tatattctct gtccatccgt ttgattttat ggataaattg
     7861 ttgctgcggc cgattaggcg ttgcttcaat cgtcaattcc tttccatttg acaaaagcaa
     7921 aacagccttg tcaattaatg gcataccgat aacatattca ccggttccag gcgtgacagg
     7981 ataaaatccc agactattga agatatacca agcggccata cttccattgt cctcatctcc
     8041 agggtaaccc attggagaat cgtcgaaggt ctgagtcatc aactgtttaa gcagtggctg
     8101 tgccatttca ggttgtccta tataactgaa caagtaagga tagtgaaaac ttggctggtt
     8161 tgaaatagct aattgtccaa attctatcgc ggccatctca ctcatttcgt ggatctcaaa
     8221 accatatcct cctgggtcaa acgttggggg ctgattgcac agctcaatga gtttttcttt
     8281 atatctctca ctgccaccat attttccgat cagtcctgaa aaatcgtgat aaacagcaaa
     8341 gctgctttgc caagcacttc cttctgcata atcttgtccc caacgattag ctgaaaatgg
     8401 ttctctgaac ttgccatctt tatcttttga acgcataaat cctgactcat agtcaaagat
     8461 attttgatag ttcaatgatt gctgctgata tttcttcgtc atttcctctt cttctagtac
     8521 cttggcaact tggctaatac aaaaatcgct ataagcatag tctaatgtgt gattgactga
     8581 ttcgtggtag tgattaggaa catagccata tgtcaagtaa tcttctgttc cttggcgtcc
     8641 ataatttgga tgtttgcttt gaaccgtagc acctttttcc attgccgtta gaaattcagg
     8701 catcaagtct cgtcggatat cttttacagc agcatctgca atgaccgcat cgatcaatgt
     8761 cccaggcata agtccacgtt catcaggaga cagccatttt ggcaaatagc cactttctct
     8821 atagctgttc aaaaaacctt ccagcatttc ttcgtatttt tctacagcaa tcaaagaata
     8881 aagaggataa accgttttat acgtatccca aaacccatta tttgtataga gaacaccagg
     8941 ttttatagat cgactagtcg tatcatagtg gattttttgc ccactaggat caatttcata
     9001 aaatgtttgt ggaaataaaa acacccgata taaattatga taaaacgtcc gcaattgttg
     9061 ttgatcatgg tgtgtgagct gtatacgatc aaaataggac tgccattgtt cttgactgtg
     9121 agcaaggtaa gtctctttgc tttggttttg ttctagttcc aggttataag ctgcttgttc
     9181 cgagctgata aaagatgtcc caaatcggat agtttgtttt tttgtctgtt caaaatacaa
     9241 cgggatcgag tcgctatttt tgttggaaac agctaactca tcaattgtta aaggatattc
     9301 aaatctcaat ataaaataaa aagaaaaatc aggatcttct gaaccagcaa aatgtgtgat
     9361 ttttccttcc actgtgtaag gatctataac aaagagctga tggcgtcccg gaaaggataa
     9421 aagtaaaccg ctgtcatttt tgctgtaatc aatagataag attccgccat acatgctagg
     9481 tatcaatgta gaacagatgc cataacgtaa ttgacagatc gacagatgag tcgggcaaaa
     9541 tatactttct tctgggcggt aagaactttg agcatggaaa agggtgttct cctgtagttt
     9601 tccattgatc ggcgtaaaca gcatatgact gaaatcaccc atccaaggac ttggttgatg
     9661 ggtaagacgg tagccttgaa aaacgcggtc atcaggatga aaccaccagc tgccgttttg
     9721 atccgtagtt tgcggtgcaa aataattcat cccaaaagga acgccagtat aaggctggca
     9781 gttcccatta gagaaatttg gctgattcgc tgttgcatgt ctagtatcta tttgaccgat
     9841 attcatttta tttctccttt gttgattgtc ttttgatgaa tgaaactgga aatttactat
     9901 cggatggtcg atcttttgtc tctaaccaag tgaccaaagc atctcccgca agcttaccta
     9961 actgttcaaa atcttgtgcg atggttgtca gtggcgggtc aacaagagct gctgcttgga
    10021 tatcatcaaa tccgataatt gaaaaatctt cagggatatg ataaccatgc tgtctcaaga
    10081 gattcatggt ctggatagcg acaacatcgt tttcacaaac aaaagcagtt acttcatact
    10141 tagtgatata agaaagcaat tcatctaatg tcgcgctttt gtcatccagt gaagtgtgga
    10201 aagtcaaacc agctttgtcc aatgcttcaa ggtaaccaag gtaacgctgt cgaacggatt
    10261 gtggatgagt ctgcgtacca aacaaataag cgatttttga atgtccctgt tcgatcaaat
    10321 aagaggcagc aagcgcaccg ccttggaaat tatcggaaag aacggtagga aaaggtaact
    10381 catagatttt tttatctaat atgacgacag ggaattcttt caatgaaagc tcaaaaagta
    10441 agtcaagatg ttgttctgta tcaagagcat aataaatcaa tccgtcaaat tcattgatta
    10501 gctcttttgc tgttttattt tgtagaaagt caagcgtcgt catcatgaca tcaatctgtt
    10561 tatcttgcat cacagggttc aatcccttgc taaaatcacc aacagacaaa tcagcgacat
    10621 atggaagaag aaacagaatc cgtcgtgttt ttgtaggaga agcaagaatc tgttccggag
    10681 aagcggattt aacgaaactg cctttcccgc gaacgcgata aataagtcct aattgctcta
    10741 attcagtcaa tgcacgtttg gaagtgattc tgcttacttt ataggtacgt gaaagttctt
    10801 tttcagttgg tagttggctg tctacaggta attcaccagt taaaatagcc gtttgcaaat
    10861 cattaaatat tgtctggtac aatggttgag tcatataaac acctcttaat gatatatcat
    10921 attcaaataa taccataatg gatgaaaaag taaataaaaa gatatttttt gaaagcgtag
    10981 acataattgg ctgtatgaag tataatcttt aaagatatat caaatagaaa gaaggtttta
    11041 ttagtggttt ataaagatat accaaaatcg gtgcagaaat ttatggaaga tatcacacaa
    11101 aaatgtgggg atatccatgc ggactgggca aagaatttta cagcagcatt tgccaatact
    11161 ctgttgacaa cagtcaaaaa gcaaaatgac ggaaccacct ttttattgac gggagacatt
    11221 ccagcgatgt ggttaagaga ctctactgca caagttcgtc cttatttagt gattgcaaaa
    11281 gaagatgagg aattagctga catgatcagc gggttagtca aaaagcaatt tttttatatc
    11341 aatatcgatc catatgcgaa tgccttcaac gaagaagcta acggtgctgg ccaccaaaca
    11401 gatcatacaa aaatgaacga ttggatctgg gaaagaaaat atgaaattga ttctttatgc
    11461 tacccaatac agttggccta tcttctttac aaaaatacag gaaaaactga tcaattcaat
    11521 caatctttta ttgaaggtat caaaaaaatc ttggacgtgt tcaaaacaga gcaagatcat
    11581 gaacactctc cttatacatt tgtacgagac acgacaagaa aagaagatac gctgatcaat
    11641 gacgggcgag gtgcaaaaac agcttttaca ggaatgacat ggtctggttt ccgtcctagc
    11701 gatgatgctt gccgttatgg atatctggta ccatccaata tgtttgcggt ggtcgtgtta
    11761 ggttatgtgg aagaaatttt tcaaacgata ctcgatgata aagaaattgt acagcaaaca
    11821 cagaaattga agaaagagat ccaagagggg attcagaatc acggacgaac ggttaatcaa
    11881 aaagggcaaa cgatttatgc atatgaagta gatggaatgg gaaacgccac agtcatggat
    11941 gacagtaatg tgcccaatct gatcgccgca ccttacttgg gatattgttc tgaacaagag
    12001 gaagattatc tgcaaacaag aagaacattg ttgagtaaag aaaatccata tttctatgaa
    12061 ggaaagtacg caaaaggaat tggatcctcc catacgccag aaaaatacgt ttggccgatt
    12121 gcattagcta tggaaggaat gacaacgtca gacaaatctg aaaaagagcg gatattgaat
    12181 ttacttgtag caactgacgc aggaacacac cttatgcacg aaggattcga tgtgaacaat
    12241 cctgaaaatt atacgagaga atggttttct tgggcaaata tgatgttttg cgaattagtc
    12301 atggattact ttgatattag agtagaaaag taggaggtat cgtaaatgaa aaagaaagta
    12361 tatatcattt ctcatagcca ctgggatcgt gaatggtaca tggcttacga acagcatcac
    12421 atgcgactga ttgaattgat agacgatcta ctagagctat ttgaagttga tccgtccttc
    12481 aacagttttc atttggatgg gcaaacgatt attttagatg actatcttca agtaaggcca
    12541 gaaaaaagac aggcaatcca acaagcaatc aatgaaggaa aattgagaat tggaccattt
    12601 tatatcttgc aagatgattt tcttatcagc tcggaatcga acgtgagaaa tatgctgatt
    12661 ggcatggaag aaagccgtaa atggggaacc cctgtcatgt taggctattt tcccgataca
    12721 tttggaaata tgggacagac gcctcaatta atgaaacaag ctggtatttc agcagcagcg
    12781 tttggccgtg gtgtaaaacc aattggcttt gataatcaag ttttggaagc agaaaactat
    12841 tcttcccaat actcagagat gtggtggaaa ggccctgatc aaacagcaat ctttggtctg
    12901 ctatttgcca attggtatag caacgggaat gagattccag tagaaaaaga agcagctctt
    12961 gccttttgga agcaaaaatt agcggatgca gaacaatatg cctctacgaa ccacttattg
    13021 atgatgaatg gtgttgatca tcaaccggta caaaaagata tatcaaaagc aatccatctc
    13081 gcaaatgaac tgtttccaga ttacgagttt attcattcta attttactga ctatctggaa
    13141 gcagttcaaa aagatgtgcc agaagactta ggctcagtag aaggagaatt gacaagtcaa
    13201 gaaacggatg gctggtatac tttagccaat acagcatctg ctcgggttta cttaaaacaa
    13261 tggaatacca aggtccagcg acagctagaa aatattacag aaccacttgc gacgatggct
    13321 tatgaagtta gtggaaacta tccccatgat caattagatt atgcttggaa gacattaatg
    13381 caaaatcatc ctcacgacag tatttgcggt tgttcagtcg attctgtcca tcgggaaatg
    13441 atacctcgtt ttgaaaaagc agatgaagtt ggaaagtatc ttgcacaaga ttccttagaa
    13501 cagctgaccg cagcaatcga tacgaccgga ttcccaaaag acagctttcc atttgtcatt
    13561 gtaaatactg caggaatgga taaaactggt gaagcagaaa taaccatcga attagaaaga
    13621 aaaagattcg ctgaaggaat acctgaacaa ctatatcaag agttagaaaa tttgcctaaa
    13681 cgtaagtatc atgtagaaac aaaaagtggg gccactatac cagctatttt atctgaagaa
    13741 actgtccaat ttggttatga tttgccgaaa gaccgtttcc gtgtgccata tatggcaaga
    13801 atgatcaaag tgactttacc attagaaaac atgccagcat tttcttggga aacattcgcg
    13861 cttgtagaag gggaagcgaa agcagaagaa aaggaaacaa tgattcatca gagtggacga
    13921 atcatcgaaa acggccctct acatttgacg attgaaaaaa atggtaccat tacaatggaa
    13981 gatcgcaaaa acaaacgaaa gttgaataat ctgcatattt ttgaagatat cggcgatata
    14041 ggaaatgagt atatctttaa acaacctttc tgtgatcagc caatcctatc cagtaacaaa
    14101 gaaaactctg aagtaaaagt attagtagat acaccggaaa tagcaaaaat aagtattcta
    14161 caggaaatgg aaatccctgt ttctgcagac gagcgtttag aaaaagaaca gcagatggtt
    14221 gtggaatttc gttatcgaaa agcagaacga tccaaagaaa aacgaatcct tcaaataaaa
    14281 acaatcatga caatcagaaa agatagtaaa aaaatcgact ttgaaacgac tctcgacaat
    14341 cagatgaaag atcatcgctt acgtgtcttg tttcctacaa agttacatgt ggaacatcac
    14401 gaagcagata gtatttttga agtggtgaag cgcccaaacc atgtgtctaa aagttgggaa
    14461 aaccccacga atccgcaaca tcagcaagca tttgtcaata ttcatgatga agagtacggt
    14521 gtaacagtcg gaaactttgg tttgaatgaa tatgaagtaa cagaagacgg acaaatcgca
    14581 gtgacgctac ttcgcagtgt gggagaacta ggcgattggg gatattttcc aacaccagaa
    14641 gcacagtgtc taggtgagca tcggttcaat tacagcattg aactacatgg accagaagaa
    14701 aaattctcta cttatttgca tgcatacgca gcacagattc ctttttccac tcaacaaatc
    14761 aaacatcatg aaggaacatt gatttcaaaa caacaatatt taacgataaa aagtgaaacg
    14821 tttgcaatta cagctttgaa acgcagcaag tttagtgata aagtagtagt aagaggtttc
    14881 aatatgtcta gtcatttaga aaaattggaa ataactaaag acaatggaaa aactgtcata
    14941 ttgaatctgc ttgaagaacc aacgaagcaa gcggttgtcc caataatcca accttatgag
    15001 attcggacaa tcggattcga ggaggaaaac gaatgtatgg cggaatagaa gcaggcggaa
    15061 caaaatttgt gtgtgcggtt tcagatggtg gagaaatttt agaaaaatta agtatcccta
    15121 ccacaacacc agaagaaaca ttggatcagg tatttgaatt ttttgatcgt tatgaattga
    15181 agggaatcgg gattggctca ttcggcccga ttggtatcga caaaacaaaa gataattacg
    15241 gatacgtttt ggcaactccc aaaaaagggt gggcacaatt cgattttctt ggcacaatca
    15301 aggaacgcta caatattcct gctgcttgga caacggatgt caatgctgct gcatatgggg
    15361 aattgaagca aggagcagca caaggaaaaa atagttgtat ttaccttacc gtgggaacag
    15421 gaatcggtgc aggtgtcgtg atcaatgaag aaattttttc agggatcgca catcctgaaa
    15481 tgggacatat ttgggtgaaa cgccatccag atgaccatta tgaaggtact tgtccttatc
    15541 ataaagattg tttagaagga ttagcagcag gaccgtctat tgaggcacgc acagggatca
    15601 aagggcaaga tctgccgcaa gatcatcctg tttgggctat tcaggcttat tatattgctc
    15661 aagcactgat caactatacg ttgacgttag cacctgaaaa gattatttta ggcggtgggg
    15721 taatgaatca ggatcatctc cttcaaaaaa tccgtcagca atttgttgaa ttgatgggtg
    15781 gttatatgga aacacctcaa gtaagtgaat atatcgttcg ttggggactg ccgaatgaaa
    15841 gtgggattat cggtagtttg ctgttagcag aacacgaaca tcaaaagtaa gcagacaaat
    15901 tacacaaaaa gaacagagtc ttatcattat ctgctgataa gactctgttc ttttttattt
    15961 ctattcccat aatccaggta aagctttacc gactattttt tcttgagtaa atggatggat
    16021 catgtgcagt tctaatgcat ggagcatcag tcgttcagct gttctatttt ggtagagagg
    16081 gtctccaacg atcggatgtc ccttatgagc taaatgaact ctgatctggt gtgtacgtcc
    16141 ggtttccaaa acacaataga cttgtgtttg ttcagaaagt actttgttga cagtgacatg
    16201 tgtcaaggcg ctctgaccat ttttatcatc tacgaccctt tttctccgat catggcgatc
    16261 tcgtccaatt ttattggtga tggtcatatc tttggcaatt tctccccata cgatggcttg
    16321 atatctccga tagatcattt tttgttccaa cattcttcca agtatcggca agacaaatgg
    16381 attttttgca aataaaacgg caccgcttgt ttctttgtcc aagcggtgga caacgtaagg
    16441 tcgttgctgt ttagtgtcga gataagcagc taaatgattg agtaaggtac tgttttctgt
    16501 cggttcattt ggatgagtct tcattcctgc aggcttatcc actataatca aatgctcatc
    16561 ttcatataaa acacggatgt gcttcgcatc tcctaattga acttcttgat aattataatc
    16621 tgtttcttcg aataacaatg tgatctggtc attgcccttt acttcatgat ggaacaaagc
    16681 aggttgttga ttgatccaaa cgtttttacg tatacgcaaa aagtgtcgaa cctttctagg
    16741 aactaaccat tccttttcaa gcaagtcgcg aattgttgtt tcaggatggt tcataggtaa
    16801 agtgatcgtg attttcacaa gtgtactcct ttcagtgcaa ttgcagacta attgtaccat
    16861 ttttaaaaca tatttaagaa aaaacttaca tttttctgtt cattcttatg ttactctact
    16921 tgcactaacg taaaaaaaca tattgaacta caacttaagc aggaagaaaa cactctttca
    16981 ctatggtaaa ataagacgat tgaatcaaat agacaggaga agaagcaaat ggattttcaa
    17041 agcattttag aaaagataaa agctgctttg atcagttttt ggcgttggat cagaccatat
    17101 ctcggacaat tccatcgatg gcgaaagcgc atctggaaaa aatatcatgt gaacaaattg
    17161 attttgctga ttggtttgat ctgtgtctta gtgacaagta tctacctctt tattttagca
    17221 aaacaagcaa atgtcgaaac gttaaaatca ggattaagcc aatcgacagt catttatgat
    17281 aagaaaaacg aagaagctgg cacactgtac gcccaaaaag gaacatatgt agaattaaac
    17341 gcgatttctc cctatatcca agatgcagta atctcgacag aagataggaa tttttacgag
    17401 catcacgggt ttgatatcaa agggattgcc cgtgcagcag tccgaatggt cttgtctggc
    17461 ggaacatcag ggggcggagg aagtacgatc actcagcagc ttgccaaaaa tgcgtatctg
    17521 acattagacc aaacgtttga tcgaaaagcg aaggaattat ttttggcaat cgagatcgaa
    17581 aagaagtaca gcaaagaaga aatcttgacc atgtatctta acaatgccta ttttggcaac
    17641 ggggtatggg gtgtccaaga cgcttccaga aagtattttg gagtagatgc aacgaatgtt
    17701 acgctgtctg aggctgctac tttggcagga atgctgaaag gtcctggtat ttataatccg
    17761 atcgatcacc caaaaaatgc gaatgatcgg aaaaacacag tactaagtgt aatggaagaa
    17821 aacggaaaaa tcacaaaaga acaagaacaa gcagaaagcc agacagatat cagcgcgtat
    17881 cttaatgaca catacgataa ttcagattct ggttatcgtt atccttatta ttttgatgca
    17941 gtgatcgatg aagcagttaa cgagtatggt cttgatgaag aagatgtcat gaataaaggc
    18001 tataaaatct atacagcttt agatcaggat tatcaaaagc aattagaagc tacttatcaa
    18061 aacagtgcat tattcccagc aaatgcttca gatggtgcca tggtccaatc tgcttcagta
    18121 gctttagatc cgaatacagg tggtgtacaa gcactagtag gacgaagagg agatcatgtc
    18181 tttagaggat tcagctttgc tacacagatg cgtcgttcac caggatcaac gatcaaacca
    18241 ttaagcgtat atacaccagc actggaagct ggatacaagc cttccagtat cttgaaagac
    18301 gaaccgcaaa gttattacga tgcgcataat tttgacggga cataccaagg agaagtaccg
    18361 atgtatcaag ctgttgcaca aagtttgaat ttgcctgctg tatggctttt gcatgagatc
    18421 ggtttacaaa aaggatatga taaagcagaa gaattcggcc tgcctttagc aaaatctgat
    18481 aagtattatg gcttagccct tggtggactg gaaaaaggag tatctccatt gatcatggcg
    18541 ggtgcttaca gtgcatttgc caatgatggt cagatgtata cgccgcatct tatcacgaaa
    18601 atcgttgatt cgactggagc ggtgatcgtc gataacacaa acaaaaaacc aaaacaggtc
    18661 atatcaaagg aaacagccga tgaaatgacc agcatgcttc ttggtacatt ttcaaatgga
    18721 acagcggctg ctgcgaatcc tgcaggttat acgatagcag ggaaaacagg aaccacagaa
    18781 actaatttcg atgccacaaa agcaaatgac caatggatga tcggttacac gccagacgta
    18841 gtgatttcga catggatggg ttacgaagaa tccagcaagc tgcactattt ggaagggaca
    18901 agtggaaatg ttgtcgggaa agtcttcaaa tcagcggcag aaggcatcct tccgtatact
    18961 tcaaaaacag gatttagtgt agcagatgcc tatgccacag gaggacaagt ggtagctgca
    19021 gatcaagttg gaaacacagg aaattctaat ggagagtctg gaaattggca agataacttg
    19081 aatcaatatg gtgaacaagc aaaagaaggg ctgaaaaact tcggcgatat ggtgaaagaa
    19141 ggcgttcagg gaattggtga agcggctaaa gacctctggc ggaaatatca aggagagtag
    19201 cccctttgaa aacgaataac cccatgttac aatagagagt aactactaaa agaaaagagg
    19261 gcataacatg tcaaacatct acgatagcgc gaatcaaatc gaacgtgaga tccgtgaatt
    19321 accagaattc aaagcgttga aagaagcgta cgagaaagta caagcaaacg aagaagcaca
    19381 caaactgttc aaagacttcc aagcgatgca aatcgaatta caagaaaaac aaatgagtgg
    19441 acaagagttc agcgacgaag atgcaatgaa agcacaagaa atggctgtga aaatccaatc
    19501 agaagaagta atcaatgact tgatgcaaaa agagcaaggc ttcagtacaa tcatcaatga
    19561 cttgaaccgt atcatcatga caccagttcg cgacctttac agcgaataaa actattttat
    19621 gaaacaacag agggtgtgaa agagacgttc ggccttgagc gatgacaaaa ttgcttctta
    19681 ttgccgatag gctgtctctt gaacaccatt taccaagagg tcgtgagcct accgtttaac
    19741 tctgagttac aaggaacaat ttcaaaaaac agcttctcat gtttgatgac tattgtgact
    19801 tgtgaccgag gagttggcag gagaacaccg tttattcaga aataggagct acgacaaaag
    19861 tcttgtcata gctcctattt tttgtcattc atttttcaag cggtagaaat agcctttttg
    19921 agctaacagt ttttcgtaag tacctatttc aacgattttt ccttcatcga tcacaacgat
    19981 tttatctgcc tctttgatcg ttgaaagccg atgagccaca ataaaagtag tgggtgtttc
    20041 tgtcaggtaa ttcagggctt gctggatttt tttctctgac tggttatcta aagctgaggt
    20101 tgcttcatct agcaaaatga tttttggctg acgtatcaac gctcgtgcaa tggagataca
    20161 ttgcttttgg ccaccagaca gcttgttgcc atgttcgcct actaaggtat ccagaccatc
    20221 aggaagctgg tcaatcagtg aagaaagttg cgcagcttcg atgacttcat ctaaacgttc
    20281 ttttgatacg tttttcaagc cataagtgat gttctctttg atcgaagcgg agaacaacaa
    20341 agtggtttga ggaacaacgg ctaaataatt tcgcacactt cgcatatcca gttcatccaa
    20401 aggctgatca tctagaaaaa tttttcctga agtcggctgg ataaatccaa tcaataagtt
    20461 aaggagtgta gattttccag agccagatgg cccaacaaaa gcaattgttt ctccagcttt
    20521 cacctctaga gaaaaatgat cgataatcgg tttgagtgag tccggataaa gaaaagaaac
    20581 atcctcaaaa cgaaaatttc catccaaatg ttggattttt ttcttaccgt gataagtttc
    20641 tacatcgcca gcaatcaaca gttcgccaat agaagtgata gattccatgc cttttgaaat
    20701 cgtaggaatc agcgtgatca ttccagaaac ggaattgacg atcgtcgtga aatacgtctg
    20761 atacatcacg acatcgccgg gttggatgcg cccttgtaaa gctaatatcc cagtgaaact
    20821 caaacaaatc agctgaaata tctgaaaact agcccagctg actgatccga aaacagattg
    20881 caggatatct aagtggaatc ctttttcata gatttgagta aaatgcttat ttaagcgagt
    20941 tgtttcgtgt ttttctaacc catgagctcg agcaatcggt gctaattcga tcacttcgat
    21001 cacttttgca gaagtctctt ccatttcttg cctgaagtct gagttggact gtctgatcct
    21061 tttacggaaa aagagagtaa cgaatgcggc gagaggaaca gtcaataaga aaaacatgaa
    21121 aacaacagga cttttataaa gcgtgatacc taaagctacg aataaattga cagcgatatt
    21181 taatccgctg acaaatactt gagaggacaa cgtttggata gcttctacat cacggatgat
    21241 ctttgactgg attcgtccag attgcaattc tttttgataa ggaatagata gttcttggat
    21301 tttcttgata agggtcgctc ttagtccagc ttctacttga cggatcgaag tgctatggta
    21361 ttttacatga aaataattcg tcaatatatt ttgaccaatc agacaagcca tcaaaaaagc
    21421 gttgaaataa atggcctgca actgagaaga atctttcgat gaagctgcat tgattacatt
    21481 ggcaatagcg atcggcatta accaagatgg aagatgtttg ataataaaaa agagtcctga
    21541 catggcaaaa cgccaataat ttccctgata aagatgcagc aatgtctgaa aagcatttgc
    21601 atgatatttt tgataacttt tctgtattcg ttcctccata atcttctcct taacaatctt
    21661 ggtaaataaa ctgcaagtgt tctattgctt caggaacata aatctcagga gtagcttcaa
    21721 gagtggcgta cattagagga cgctcatatt tgatataatg cagtaccggg cggaaatcta
    21781 aacagccctt gccaacaggt accatgcgga tctttccatt ttccatgata tagtctttga
    21841 gatgaagcgc tgtaatataa ccatgtaaat tgtccaaggc acctcggatc actttttcct
    21901 gatcagcatg attttcttta tgcatcaaat tcgcacaatc taaaatgatt tttaaatttg
    21961 gtgaagctac ttcatcgatt aaacgtttag ctaactgata gttatagatt ggatgattga
    22021 tccctgcttc aacagcaaca gttacgccaa aattttctgc atgagcgact aattcaatga
    22081 tagaatttct ggcgatctga taagcttctt cagtaaaatt ttcttttgtg taaccgacag
    22141 agacactacc ggtttccgta gcaatcatct tggcttgata ggcagagcac agagcaattt
    22201 gctgtttgaa agttgccaat gcttgtgcac gaacttcttg atttttgctg gataggttga
    22261 tatagcatcc taaaatggaa agttcgatat tgtttttttg gaagtattca ccaaaatagg
    22321 cagcactccc aagtgtcatg tcagttaatt taagttgttc aggaaatgct ttttgtggtg
    22381 caaattggat atgggacaag tgaagctgtg atactttttg aacgatttct tcacgatttc
    22441 gaacatctaa atcgtgggca cggatgccta aatttaatgg aaacatctca aactcctttt
    22501 tgtaaataat tagcaagagg cagacgggat tcttttatca attcaacaat tattcgggca
    22561 actttatcag cgccttctgg actaaagtgg gtattgtctt ttagtccttc tgggtaattt
    22621 ttacaagtat caggcataag atgcaggtag aatttttttg tctcttcttt tgagaaggtc
    22681 tggaacaatt cttgtgatct tgagaacaca tctaaaagag gaatatggtg tttctcagca
    22741 aatgcacgca tcgcttttgg atagtctcca agtatatctg gattcaatgt tccgttttct
    22801 aagtaatctc gtcgcgtcac agatgtcagt aagaccattt ggacgttcgc tttttttgcg
    22861 cattcaaaaa atatctgcag attttttaaa tagctaccga agggaagcgt acctctttct
    22921 tcttggattt tctggtcgtt atgtccaaat tgtacaaata agaaatcatt cggctgaaac
    22981 tgtgaagcta gacgggctaa ccttccttcc tggataaatg atttggtgct gcgtccattc
    23041 tttgcttggt tattgatctg gatctcgggt gaaaaataag cagagatttt ttctccccaa
    23101 cctgtctcgg gacgcttgtt ttcatctttt tctgccgcag tagaatcacc ggcaatatgg
    23161 atagtaggca tgttatctct tctttcataa aggtttatag gcaatgaagc agaacagcaa
    23221 gagaagaaga cggagcaa
//
LOCUS       DAJSKS010000033        23194 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJSKS010000033 DAJSKS010000000
VERSION     DAJSKS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.034"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(25..603)
                     /locus_tag="ON396_001095"
     CDS             complement(25..603)
                     /locus_tag="ON396_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3113196.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(680..1210)
                     /locus_tag="ON396_001096"
     CDS             complement(680..1210)
                     /locus_tag="ON396_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCR3113197.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1214..2074)
                     /gene="rlmB"
                     /locus_tag="ON396_001097"
     CDS             complement(1214..2074)
                     /gene="rlmB"
                     /locus_tag="ON396_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR3113198.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2067..2462)
                     /locus_tag="ON396_001098"
     CDS             complement(2067..2462)
                     /locus_tag="ON396_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCR3113199.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2459..3871)
                     /locus_tag="ON396_001099"
     CDS             complement(2459..3871)
                     /locus_tag="ON396_001099"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR3113200.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFLQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3868..4404)
                     /gene="cysE"
                     /locus_tag="ON396_001100"
     CDS             complement(3868..4404)
                     /gene="cysE"
                     /locus_tag="ON396_001100"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR3113201.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4490..4729)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4726..6186)
                     /locus_tag="ON396_001101"
     CDS             complement(4726..6186)
                     /locus_tag="ON396_001101"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR3113202.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6356..7513)
                     /locus_tag="ON396_001102"
     CDS             complement(6356..7513)
                     /locus_tag="ON396_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCR3113203.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7547..8917)
                     /gene="radA"
                     /locus_tag="ON396_001103"
     CDS             complement(7547..8917)
                     /gene="radA"
                     /locus_tag="ON396_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR3113204.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8980..9507)
                     /locus_tag="ON396_001104"
     CDS             complement(8980..9507)
                     /locus_tag="ON396_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR3113205.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9737..10543
                     /gene="proB"
                     /locus_tag="ON396_001105"
     CDS             9737..10543
                     /gene="proB"
                     /locus_tag="ON396_001105"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR3113206.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10540..11787
                     /locus_tag="ON396_001106"
     CDS             10540..11787
                     /locus_tag="ON396_001106"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR3113207.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11863..12207
                     /locus_tag="ON396_001107"
     CDS             11863..12207
                     /locus_tag="ON396_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113208.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12324..12650)
                     /locus_tag="ON396_001108"
     CDS             complement(12324..12650)
                     /locus_tag="ON396_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113209.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13265..14467)
                     /locus_tag="ON396_001109"
     CDS             complement(13265..14467)
                     /locus_tag="ON396_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3113210.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14584..15099
                     /locus_tag="ON396_001110"
     CDS             14584..15099
                     /locus_tag="ON396_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3113211.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15298..15744)
                     /locus_tag="ON396_001111"
     CDS             complement(15298..15744)
                     /locus_tag="ON396_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCR3113212.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15791..17269)
                     /gene="msr(C)"
                     /locus_tag="ON396_001112"
     CDS             complement(15791..17269)
                     /gene="msr(C)"
                     /locus_tag="ON396_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCR3113213.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17623..18252)
                     /locus_tag="ON396_001113"
     CDS             complement(17623..18252)
                     /locus_tag="ON396_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR3113214.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18236..18700)
                     /locus_tag="ON396_001114"
     CDS             complement(18236..18700)
                     /locus_tag="ON396_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3113215.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19187..20455)
                     /locus_tag="ON396_001115"
     CDS             complement(19187..20455)
                     /locus_tag="ON396_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR3113216.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20686..21423)
                     /locus_tag="ON396_001116"
     CDS             complement(20686..21423)
                     /locus_tag="ON396_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3113217.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21490..22059)
                     /locus_tag="ON396_001117"
     CDS             complement(21490..22059)
                     /locus_tag="ON396_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCR3113218.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22187..23059
                     /locus_tag="ON396_001118"
     CDS             22187..23059
                     /locus_tag="ON396_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3113219.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
BASE COUNT         6514 a         4951 c         4199 g         7530 t
ORIGIN      
        1 gaagctcatt aagatttttc taaattattc ctcattataa aatttcaagt gtttttttat
       61 tttgagtttt actctcccgt aagcgctcat tacttgcttg tgatccattg ctaaacattc
      121 agccatgtct acaaaatcaa cgccttgcat ataataatga aaaattttag cttctatttt
      181 tgaaaactgg atttcgcaat tttcgattgc ttcttttaat aaaagatgct gaattgctaa
      241 aggttcttgc aatgtgatct ctgctaaaaa gtctggtcct tctgtctcta ttttttggtc
      301 gattgagact tcatcttttt gatcacgacg cttcagcgcg ttctgcttcc tcaacagaga
      361 atagatttga ttgcttataa cgcttttata ataacttcca aagctaacac ctttgctttt
      421 ttcgtaagtt ctcaatgcct gatataaagc gatccttgct tcttgttccc aatcctcaga
      481 aacataattt cttaaataat aggtgttttt caaacgtagt aaaagtggaa aatatttttg
      541 atacagggca gcaaatgctt cttcgtcacc attacgtgca gcgcgcaata actcctcaga
      601 cataaaataa ttcctccata aattcgttta tggaggaatc ttatcatcaa tgattcatcg
      661 aactgattag aacgatatgt tatttatttt ttctcattaa atcgtccaat ttttgggaca
      721 atttttttaa ctgctcttca ttccatggag aattacgtcg gaaatcaata aaatgcatat
      781 ctttattttc ttgggctatt gttttttccg ttttttttat cgtcttatat aattcataag
      841 cagaagttcg taatgctcct tgggaaaaga tcatccactg ttctgctaaa tcgcttgtcg
      901 ccactgtcac ttgtgtcaaa cggtcattca gctgaccagc gattctctcg atataactgt
      961 cagccgtttc gtcttctttc gtaaaaacaa catccaactg gtatttctta taattttgtt
     1021 ggatgcccgg aactagttgg gcatcgaaga caacgatgac ttccattcct tcatatttcg
     1081 cataatttga taaacgatgt aaaagagctt ctcttgcatc cgccattttg tcctgctttt
     1141 taagctggac aagttccggc cacgaaccaa tcatgttgta gccgtctaca atcaacagct
     1201 gttttttcat ggtctactcc ccctgacgtt gggaaaaagc ctgatacata agcaaaccag
     1261 cagcaacact ggcatttaag ctctggacgt gcccggtcat cgggatcgtc agcaattcat
     1321 ccatttcttt tttcaatcct ggactcattc ctttgccttc attaccaatg actaaagcaa
     1381 ttgatccttt ggcattccat ttttggaagt ttgtgccggt catatctgtg ccaaataccc
     1441 aaaatcctgt ttctttgagc tgttgcacag cttgtaccaa attcgtcacg cgtgcaacag
     1501 ggacatgttc gactgcccca gtggaagtct ttgttacgat aggtgtgatt ccaactgctc
     1561 ggtgttttgg tatgatcaca ccatcgacgc cacttgcgtc tgctgtccgt aaaatcgacc
     1621 caaaattgtg tggatcttcg atgctatcca aaatcaaaaa gaaggggtct tcttttttct
     1681 cttttgttcg ttcaatcagt tggtcaaggg ataaatattc ataaggtgta atcgcaagaa
     1741 cgactccttg atgcacgccg tgatcgctca aagtttctaa tttttgttta ggaacccatt
     1801 tgactggcac agcttgttca cgtgcgattt ctttcagttc ttctactttt tctcctcgtg
     1861 aatcttcttg aagaaagagt ttattccctc gcccttgttg tagcgcttct actgcggcat
     1921 ggtgcccaaa gacgaaatta tcttctaatg cttcttcatt ttgatcagaa actgactgat
     1981 ttcgtccagt ttgcttttta tttgaatatc tattttgttt tttgtcttca aatccacgtt
     2041 tcgattgatg tttgtttgtt ctttctttac ccattttttt caccaacttt ctgaatacac
     2101 cagcggatca attcttccat ccgctcggtt tgttttgtca aatgcagata gcccatgagc
     2161 gcttcgaacc ctgttgcgac ccgatacgtc gtaacatcag cattttttgc gctcgtatgg
     2221 cttttagcat ttcttcctct gcgataataa agttcttctt cttctgtcaa aagatgttct
     2281 tccagcattt gctgaatcaa aaaggcttgt gcattcgcag aaacataatt cgttgccgca
     2341 cgatgcagct tattgggttt tgtttctcct tgatcaatca ggtggtcacg aacatagatt
     2401 tcgtaaatcg catctccaac ataagccaag gctaaaccgt tcaattgtgt gtaatctctc
     2461 atgcttctct tctccatcgg gttccttgcg gtgtatcttc taataagatc cctttttctt
     2521 tcaacaaatc acgaatttcg tcgcttcgga caaaatcacg attgcttctt gcttgatttc
     2581 gttcttcgat caattgttca atctcttcat ctaataactc ttctgctgta aataaaatac
     2641 cgaagatcga aagccattgc aagaataaag cttctccttg tgccaagaca gcggcagaaa
     2701 ctttcggctg gtctagataa gtgttcagcc acttagcaag ctcatagacc actgtgatcc
     2761 catttgctgc attaaaatca tcgtccattt cttcgatgaa tcgctgttct aaattttcta
     2821 acaaagcgat tttatccgca tcatctgcca aattctctga ggcagtttct tggcggaatt
     2881 tcaaattgtc aaacgtattc ttcagtcgtt gtaaattggt ggctgcttct ttcatcgtcg
     2941 tttcactgta gcggatcgga cgacggtatt gtgtggtcgc catgaagaaa cgcaagactt
     3001 gtggatcaac gtttttgatc atttcatgta cagtgatgaa attccctaga gatttactca
     3061 ttttttcatc gtcttcgcca atcgttacat agccattgtg catccagtaa ttagcgaatt
     3121 ttttccctgt tttcgcttca ctttgtgcga tttcattttc atgatgagga aattctaaat
     3181 cttgcccgcc accatggata tcgatcgttt ctcctaaatg ttttgttgcc ataacggaac
     3241 attcaatatg ccaaccggga cgtcctttgc cccatggtga atcccaagaa atctcgccag
     3301 gttttgcttg tttccataaa gcgaaatcta atgggtcttc tttttgctgc tgttcttccc
     3361 ctgtacgctg acttgctccg acttccagtt catcgatgga ttggtcactt aatttcccat
     3421 aattttcaaa tttacgggta cggtaataca catcacctga agattcataa gcatagcctt
     3481 tttcaaccaa cacttgaata aatgctaaaa tatctggcat atgatccatg actcttggat
     3541 gcagtgtagc aggttcgaca ttcaacgctt tcgtatcttc ttcaaatgct cggatgaaac
     3601 gatcagcaac ttctggtgct gtaataccta attcgtttgc tgtacggatg attttatcgt
     3661 ctacatccgt gaaattcgat acgaaatcga ctttatagcc tcgatattca aaatagcgtc
     3721 gaattgtatc gaaagcaatg gtgctacgag catttccgat atggatataa ttatacacag
     3781 tcggtccgca gacatacata ccgacatgtc ccttttgtat agggatgaat tcttcttttt
     3841 ttctggtcaa tgtattgtag atttttatca ttctgatctc ctcacttttt ctcctttgat
     3901 tcgaacgact tttgctggta ctcctaccgc tgtagcgtcg tctggaacat ctttcaacac
     3961 aacggcacct gcaccgattt tggcgttttt tccgatcgtt accggtccta atatctgcgc
     4021 attcgcagag agcatggctc cttgtttaac agttgggtgt cttttacccg tttcttttcc
     4081 tgtgccgcct aatgttacgc catggaataa aatcacatcg tcctcgattt ctgcggtttc
     4141 gccaatgacg attcccattc catggtcgat gaacacgcca gttccaatcg ttgctcctgg
     4201 atggatctca acaccagtta ggaaacgcca gaattgagca ttgatcttcg ccaataaaaa
     4261 gagccggtgc tgatataaaa aatgagagaa acgatgccaa aacaaggcat gaaaaccagg
     4321 gtatgtcaaa acgatctcag ctgtcgaacg tgcagccgga tcgtttctct ttgctgcatc
     4381 aatcgctcgg gtaaaccaac tcatgacttt ccactccttt cgtaagaatt ttatgtagga
     4441 aatcatcttc ttataaagaa aagaagcgcc ttttaaataa gtttccttat caaaagacgc
     4501 tttccgcgtg gttccacttt tcttcataat caatgattat ctctgacagc taacgcctgt
     4561 tatacgttct tggctacttt gtttcctata aaacagtttt tcctgttttc tgtgtttcgc
     4621 caagaccact cccagaggca tttcggcaac cattcattga taccttgcac cacccggtat
     4681 ctctcttgta atgatcagct gcttactttt ttctgttcat cgtgtttagt tcagtacttg
     4741 atttaaatgt gctaatgatt tttcacgacc aagcaattcg atcgtttcag gcaattctgg
     4801 accatgcatt tgtcctgata cagctacacg gattggcatg aaaagatttt ttccttttac
     4861 gccagtttct ttttgaacag ctttgatggc agctttgaca gttggagcat ctactacttc
     4921 catttgttct agctgtgctt tgaatgcgtg gagaacagta ggaactgttt caccagcaag
     4981 tacttctttt gctgcttcat tcaatacagg atgttcattg aaaaatagcg tagacagatc
     5041 aacgatttct gcagcatagc tcatttgagg ttgatacagg ctaacgatct tcttcaccca
     5101 ttcgattgtc ttttcatctg ggttttctgc tacacgtcca tcagcgatta gataaggcag
     5161 acacatgtca gtaaatgcat ctagatccat tgctttgatg taatggttgt ttacccattg
     5221 cagttttttc gcatcgaaag ctgcgggtga tttacttagg cgttttggat cgaacatttt
     5281 gatcaattct tggtgttcga agatttcgtc ttcgcccaca ggtgaccagc ccaataatgc
     5341 aataaagttg aacattgcat ctggtagata gcctaattcg cgatattgtt cgataaattg
     5401 caagattgat tcgtcacgtt tgcttagttt tttacctgtt tcactgttga tgatcaatgt
     5461 catatgtccg aaggttggtg gtgtccagcc aaatgcttca tagatcatca gttgttttgg
     5521 tgtattggca atatggtcat ctccacgtaa aacgtgagtg attttcatca aatgatcatc
     5581 gaccgcaaca gcaaaattat aagttggcat accatcgcgt ttttggatga caaagtcgcc
     5641 accgatatta tcggattcga acgaaatagc gcctttcacc atatctgtaa atgcgtattc
     5701 tgtattgcga ggcacacgga aacgtacaac agattctaga ccttgtgctt ctttttctgc
     5761 ttgttcttca ggtgtcaagt tcgcacatgt tcctgcgtaa tgaggcattt ctcctcttgc
     5821 gcgttgtgct tcccgttctg cttctaattc ttcttctgtg caatagcatt tgtatgctcg
     5881 attgctagca agcagctgat caatcagcgg ttgatagatt tctttacgtt cagattgacg
     5941 gtaagggccg tattctccag gattttcagg agattcatcc caattcattc ccaaccaagc
     6001 aaggttttcc aactggcttt tttcgccatc ttcgatatta cgtttttggt cagtatcctc
     6061 aatacgaata ataaagtctc catcattgtg gcgggcaaac aaataattga ataatgcagt
     6121 tcttgcattt ccaatatgca ggtgcccagt tggacttggt gcatagcgta cgcgtacttt
     6181 cgtcattttc ttcctcttct ttcaaactag actttgacta acagcaaaaa ttccgtcagt
     6241 tacggcttct caaatatcat ttatttaaag ccactcagca caaattttac cttttctaag
     6301 gtaaatagta aagccaactg gttgaatttc cttaccagtt ggctgaattt cttttttatt
     6361 ttgattgttt agtctttttc tctgtctctt ttttattgtc gattcctcgt cctgcatgtg
     6421 caggtttagc aaagatcatg cgtccagcag ctgtttgaag ggcacttgtt acaacgactt
     6481 cgatacgttc attcatgtaa tgctgtccat cttctacgac aaccatcgtg ccgtcatcaa
     6541 ggtaggctac tccttgttgg cgttctgtcc cggcttttac tactagaaca ttcatcgttt
     6601 caccagggat gacgactggt tttacggcat ttgccaacgc attgatatta agaacaggta
     6661 cattttggaa ttcagagact ttattcaagt tatagtcatt ggttacaatg acaccatcta
     6721 aaagcttagc caatttgatc aatttgctgt ctacttcact gatatcttca aagtctccat
     6781 cgtacatttc gacagatata ccctcttctt tttgcaaggc atttaggata tctaatccgc
     6841 gacgaccacg cacacgtttc aaactatccc ctgagtccgc gatgtattgt agttcgtaaa
     6901 gaacaaaatt agggatcatc aaggttcctt ctaagaatcc agtctttgta atatcataaa
     6961 tccgaccgtc aatgatcacg cttgtatcca agattttata tttatggaaa ttttctcctg
     7021 attttcgttc taacagttga ctctcaactt gactagctgt ttcttgctcg atttttttgc
     7081 ttcgtgaacc aaaaactttc ttccattctt cgattcgtgt cgttcccatt ctcaatccca
     7141 gatagcctaa gatgatcatc agtaaaatcg gcaatacact attgacgaat ggaatttcca
     7201 tattgtaaag aggggtcgag atcacaacac ctaaagtcaa tccgatgatc gcaccgatcg
     7261 caccgaagag aagataagtc aagctgacct cacttagttt ttgttctact tgtttgataa
     7321 tagctgagat atgtttggcc aacagcaaag ataataaaaa gaaaataagt gcgcctaaca
     7381 ggctgttggt aaatgtatta ttcagccatc cgtttgactg ttgaccgatt gcctgccaag
     7441 ctgagggtaa aaatgaaata cccaagcttg cgccgacaat gatcatcagc aaagtaatga
     7501 cacgtttttg catcgttttt cctccgtttc gacaatcatt tattagttag cggaacacac
     7561 gcttcaacgt ttcagccaaa gttgctactc caactatctc gattcctttt ggcgcttccc
     7621 agttacctaa gttgttttta ggtaggtata cttttgtgaa gccgagtttt tggacttctt
     7681 tgactcgttg ttcaatatga ctgacacgtc tgatttctcc agtcagtcca atttcaccga
     7741 tgaaacattc cgttggctgt gttccttttt ctttatagct tgaagcaata ctgacagcga
     7801 tagctaaatc aatcgctggt tcatttaatt ttaccccacc tgcagctttt aagtaagcat
     7861 cttgattctg aagaagcaac ccagctcttt tttccagcac tgccatgatc agagataccc
     7921 gattgaaatc aagacctgtt gttgttcgtt tggcatttcc gaacatagta ggtgtgacta
     7981 atgcttggat ctctaccaaa attggacgtg tcccttccat tgccacaacg atagcagagc
     8041 cagttgcacc gtctaggcgt tcttctaaga aaatctgtga aggattagcg acttcttcca
     8101 atccatgctc atgcatttca aaaatcccaa tctcattcgt tgagccaaat cggtttttta
     8161 ctgctcgcaa aatccgaaaa ctatgatgtt gttcaccttc aaaatataac accgtatcca
     8221 ccatatgttc taacattctt gggccggcga tcgagccttc tttggtaaca tgaccgacga
     8281 taaaaatggc tatcccattt gttttagcgg ttttgagtag ttctgcagtg gtttctctta
     8341 cttggctgac acttcctgcc acacttgtga tgtctggctg cgtcatcgtt tggatggaat
     8401 caatgataac ataatcaggg gagatatgtt cgatggcacg actgatttcg ttcatatctg
     8461 tttcagcgta tagataaaat tctgtgttga tacttccgag gcgttccgcg cgtaatttta
     8521 tctgctctgc gctttcttcg ccggatacat ataagacttt tccgcctatg gctgctaatt
     8581 gctgagaaac ttgtaacagc aaagtggatt tcccgatacc tggatcgcca ccaatcaaga
     8641 ccatagaccc tggcaccaca cctccgccta acacacggtt cagttcttct aattttgttt
     8701 tgatccgagg ttctttttta gggacgacgt ctgcgatttt tgttggtttt gctttttctc
     8761 ctgtcaaact tgtccgagtt ctgcggtccg ttgtatcttg ttcgatttct tcgaccatcg
     8821 tattccattt tccgcaattt gggcagcgtc ccaagaactt aggcgacaca tacccgcaat
     8881 tttggcaaac aaattgtact tttgattttt ttgccattgt accgcctcct tttatcctaa
     8941 tcatgagaca ttaaatcaag cttaagtatc tttaacgttt tatttactgg aagaaccaaa
     9001 tccgccttga cgttgagcag tcgtttgatc ttgatctgct tttaaaaacg gcaaaaagat
     9061 cccttggccg atacgctcgc cttttttgat cactacatcc ttgaaaccaa agttggtgaa
     9121 ttggaacatg atatggcctt catttgcctc attattgtaa taatcactgt caatcacacc
     9181 tactccgttt gtcaacacta aaaaccgctt caacggattg cttgaacggt tagcaagctg
     9241 caaaaattca tcttctccca tgtaagcttt gattccagta ggcacgaggg ttggtttcaa
     9301 ttgtttttca ttttctgata catctttttt caatccatct gcgactgttt ccaacccctt
     9361 tttccagcct gctttccaga tacttggaat cacgacctct tctgctgctt caaaatcata
     9421 gccagcagca tgatgtgtag cgcgctgcgg tagattgata ttttgatctt tatatttaga
     9481 aataacttca aatcctcttt ttctcaccgg ttttcctccc taaataaact actcctattc
     9541 taacatatcc gaggctgtaa aagactttct ttacaaattc ttcaagaaat tctgctatct
     9601 taaagtaaac cattaattcg ttctttttcc aaacgactgc aagttgtaca aaaaaactat
     9661 tttttacatg actttactcg tcaaaacaag tagaatagaa cgaaattaat gagtggatat
     9721 ttgattgagg tgtcacatgc gtaaagaaat taaaaatgca aaacgaatta ttgtcaaagt
     9781 aggaacaagt acattgattt atcccaatgg aaatatcaat cttgcggcaa tcgatcagtt
     9841 agcttttgtc ctttctgatc ttagaaatcg gggaaaggaa gtgatcttag tctcatcagg
     9901 agctgtaggc gttggtatgc ataaattaca tttgtcagaa cgtccgactt tgattcctga
     9961 acagcaagct gcggcggctg ttggtcaagc cgaactgatg aatatctata atcaaagatt
    10021 cagtgtatat aatcaacaga cggcacagat cctcgtgaca agagacgtgg tagattatcc
    10081 tgaaagtcgt aaaaatgtga ggaatacatt ggaacagctt ttgattatgg acacgatccc
    10141 tatcgtgaac gaaaacgata ctgtagcagt ggatgaatta gatcatctga caaaatttgg
    10201 cgataatgat cagctgtcag ccatcatggc aaaacttgtc gcggcagacc ttttgatcgt
    10261 cctttctgat atcgacgggt tttattcaga taatccgact acaaatcccg aggctcagat
    10321 gtatcgctat atcgataaaa tcgatgaaaa gattctggca caagcaggtg gtgctggtag
    10381 cacatttgga acaggcggta tgcagagtaa attaaaagcc gcttctagga tatttgaagc
    10441 aaacagcgca atgattttag caaatggaaa aaatccggca attatttttg atattcttgc
    10501 aggaaaagaa gtaggtactt tatttaagga ggacttgaaa tgactgactt gatgcaatta
    10561 ggacaacagg caaaagatag cgcgcaacaa ctagcactta tgcctacaaa ccgcaaaaat
    10621 gagctacttg ttcaaatggc acaaaccatc aaaaagaatc aacaagcgat cattgaagca
    10681 aatcataaag acttagaaaa agcagtcgaa aacaatatca gtgaaacgat gcaagaccgc
    10741 ttgcagctga cacctgagcg aatcaatgcc atggctacag agatcgaaaa aattgcttcg
    10801 ttagacgatc ctattggaaa agtagatgag atgtggacga acaccgacgg attgagaatc
    10861 ggcaaaaagc gcgtgccact aggcgtgatc ggcatcattt acgaatctcg tccaaatgtc
    10921 acgacagatg cagctagtct agcattcaaa tcagggaatg ccgttatcct tcgcggtggc
    10981 aaagaagcat tcttctcgaa tcaattatta gtccaattat tgcagcaagt actggtcagt
    11041 gctggcgaat ctccttatgc catccagttt gtcgatgata cttctcatga aactgcagga
    11101 cagttgatgc gattgacaga atacttagat gtgctgattc ctcgaggcgg agcaaaatta
    11161 atccaacgag tcaaagaaca ggcaacggta cctatcattg aaacaggtac aggaaataat
    11221 cacgtatata tcgataaaga agcacagtta tctatggcag tgaacatcat cgtcaatgca
    11281 aaagcttccc gtccatctgt ctgtaacgcg gcagaaacat tgctgatcca ttctgagatc
    11341 gcaccttttt tcttgccagc tattgaaaaa gaactggtag aacacggggt ctctttacgt
    11401 gcggatgctc gtgcgttaga atatctagag acggctgctt tagcagaaga cacagattgg
    11461 gatactgaat atctagatta tatccttgct gtcaaagtgg tcgattcttt ggatgaagca
    11521 attgcacata tcaatcggca taatactaaa cattcagaaa cgatcgtgac agatagttat
    11581 gcggctagtc aacgtttctt aaatgaagtg gacgcggcgg ctgtctatgt caatgcctcc
    11641 actcgtttta cagacggatc ggtatttggt tttggtgcag agattggtat ctctactcaa
    11701 aagttgcatg cccgtggtcc aatggggtta aatgaattaa cttccacaaa atatatcatt
    11761 tatggcgatg gacaaatccg cgaataatgt ttcgtgtgaa acattattcc cgccttttgc
    11821 cttttttctg taaaggttct atccaactag aaaggtgtca atatgtttga aattttaatg
    11881 atttttttta tttaccttat ttctctgaat atcgctgctt ttctgggagt cggtatttta
    11941 tcccttttct ttcaattcaa aaaacgaagc atagggagtc aacgggaaaa atggtcgcag
    12001 tattttgaca aaatcgggca aaaagggctg gttacacgct tgcacattag ttacatggtt
    12061 gcattatgct tattagcgac aatcaattac tattcttttt tcgatcattc aatcgcttat
    12121 acgattacgc tactgattgc tgggattttt cacttatctt ataaatatca gctgaataaa
    12181 aaccacttga atcggacttt ccgctaaaaa atatccgtac gataagtaga gtgccttgaa
    12241 gatcggcaaa aaactgccgg actttttaga ggctgtgaca aagattttgt cacagcctct
    12301 ttttccatat aaacggcgtt cgcctaccag ctgcctggta aatctgtttc ctcttcgtta
    12361 agaagcgaaa atccgtcaaa tgtttcgcca tcataataac gaagcagctc atccatggac
    12421 aagttcatcg ttttttcttt ttcttcttcc gctgattcag ccagtagctg ttcccgatac
    12481 acctgataca taccagctat tttttgctgc atcgcttttc tagtttcacc ttccgcattg
    12541 attattggca attctttgat atagccacga taaatcaacc gatcatctga aaattcaatg
    12601 ggtaattcaa cgagtgtcaa cccgacttcc tgaaaatatt cttccagcat ctattatccc
    12661 cccattcttt ttcttaaatg aattttagct tacctactga aaattttcca gtttcattat
    12721 tttgagaaaa aaagggaatg tgacaaaaat cgtgtcacat cccctttttc cgaataaatg
    12781 gtgttcttct gccaactcct caatcataag tcacaatatt caacaaacat gagaaactgt
    12841 tttttgaaat tgttccttgt gattcagagt taaacgacag tctcacaacc tcttggtaaa
    12901 cggcgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggtaaaata tgagaagcta
    12961 tttttccgtt ttgtttctct aatacttagg ataaaacgct gattttacaa cctctgttaa
    13021 acggtgttca atcagctgtc cttcggtatt agctcaaaaa acggcaaaat atgaggaact
    13081 atttttccgt ttgtttctct aatactcagg acaaaacgct gattttacaa cctcctgata
    13141 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagaa
    13201 gctatttttg tttggctgtt cttatgactt gatgcaggac agctttttca caacctctga
    13261 tttcttattc ttcttcattt gtagcaaaat aattacggtg ataaatgata caacatacac
    13321 tgataagaac aatggcacct gcaaaataaa acgcatagct ttcaccaata tgttcaccaa
    13381 tcacacctgc aataaatggt gctaaagctc caccgaaaaa gcggacaaaa ctatagctgg
    13441 acgaagcaac gtttcgttgc agacctggta tttccatagc aatcgtcgtc aacagtgtat
    13501 tgataattcc ttggaaaaat cctgctaaca caatgctgat cggtacaagg actggcatag
    13561 aagttccagc tcctaataac agcaaacaaa taatgaataa gacaaatgct gctaaaattg
    13621 tccgataagt cgtaaacttc cgctcaagag ccggtgcaac gaaaatcgaa gcaatagcca
    13681 gaagcacacc ccagccaaaa aatacaaaac cgacttctaa ttcagaatag ccaatcaata
    13741 aaaatggcgc atacgcaagt aaagtaaaga atccgaaatt atataacaat gcgattagtc
    13801 caactgaacg aagtttatga ttgcttaacg ctttgacacc ttcccaaagg cctacttttc
    13861 tctttggttt ctcagtttca ggtaaaagta ccgctagggc tataaaaccg caaaacatca
    13921 aaaatgctac cccaaaaaat ggataacgcc atgaaccact gcccaagacc ccaccgacta
    13981 gagggccaac agccattccg cagccaagtg ctgcttcata catcatgatt gctttttctg
    14041 tgttaccaac taaaatactg acgatcgttg ctaaagcagt agagacaaat aaagcattcc
    14101 cgacacccca gccggcacgt aacccaatca gcattccgat attcccagat aatccgccaa
    14161 gaccagcgaa tacaacgatg ataaacaatc ctgtcatcaa tgtttttttc aaaccgatcc
    14221 gactagataa gaaacctgta aatagcatca ccacaccagt gactaacatg taactagtaa
    14281 ataacagggt cgtttcagct ggcgtagcgt ttaactgtgc agcaatcgtt tttaagattg
    14341 ggtctactag acccactccc ataaaagcga tcatacaggt agctgctaca actagcgctg
    14401 aacttttccc ctttgtttct tcatttgttt gttcttgtat atctatttga ttctccaata
    14461 tttccatccc tttctctcta ttagcatcat acatgattta ttttacatgt caatatgcac
    14521 atgtaaaacg tggaaacttt actgtgatat aataaagaaa ataagacgag aggtaggatt
    14581 tttatgaata ctttagccta tatattatta tgcgtgcttt tatccaaacc acgttctggg
    14641 tatgaattga agcagctgat cacaattttt tgggaagccc atcacagtca gatctataca
    14701 acgcttgcca agttagacca gcagggttat gtgaccgttt tggacaaaga cgagcactct
    14761 caaaaaaaga tctaccactt gactgatgaa ggacgtgctc ttgtagagga atggatcaaa
    14821 gaggaaactc ccgctcctac gcaaaaagat gaatttctag caaaaatcta tgctttttcg
    14881 acgctcgata aaacaacagc tactggactg ctattcacta gagaacaaca gctgaacaaa
    14941 atcatgcaaa aaaatcaaaa aaaattgaat gacctctcgc tagacaagaa acaagaattt
    15001 ggacgttatg ttgtgatcca gcgcagaatt atgctttgtc agcaggaact ccaatggtgt
    15061 cagcaagtaa gagaacaaat gcagcagttt tttgagtaag ggataaacag aacctttcta
    15121 gaaatatcta ttgaagagca aattatcttc aataaaagag caatacagtt ggaaaagaaa
    15181 aagcgatccg aacgacaggc caaaacctat cgttcggatc actatttttc tagaactgca
    15241 ttcaagagac tatgacaaaa agcatgccaa ggcctttttt caaataacag tgttccatca
    15301 ttcattactt tgaattgctt ttagcacact tcttgaaagc gggccaacga tgatcaactg
    15361 caacggtaaa gcggcaatca cattcatttt ccaagcattc atataagcag agaataattg
    15421 atcaggtatt ccttgttcca tgatcacacc gaataaagac atacatgtca ccattcctaa
    15481 aaccatcaag catgaaatag ttaagatcat ttggaccttg ctttctggat ttactggtag
    15541 tttaaaggca atttttttcg ccagcacacc gatgatgaag acatccaaaa tgaaagccac
    15601 cacgaatcca ggcactagac caatcaacaa acttgataaa tgaagatctt gatgcagcca
    15661 taaattatac gcgctcatcc ccaagaccat caaaaaacac atcattgttg taaaaattat
    15721 tccttctttt ttatttgtag gcaatctgta ccactccttt aaatttgtca cgaaaatcat
    15781 cgtagcacat ttaaaaattt ctcgtaagta ctttttttat tttctgccct tttatttcat
    15841 aaacttcatc agccgtacgc tcgacaaagt aagaatcatg agaagtaaac aaaatcgttc
    15901 ccggatatat ttgcattagc ttctctaaag cttcgatcgt tgccatatca ataaaattag
    15961 tcggttcatc taacagcaac acattacttg gcttagtaaa aagcaaagca atcgtcagac
    16021 gggtcgcttc tcccccactt aatgtacata acggtttcgt caaagtttcc tgagcaaacc
    16081 ctaagtgatt cagcaaacta cggacaagcg attctgaaaa ctctgtttgt ctcatgacaa
    16141 aacgaatgat ggattcttca gatgtcattt gataaccctt ttgctgatat acttgaaagc
    16201 ttactttcgg agagagaagg attccttctc cttgtttgcg gatatgttct aataaggttg
    16261 tcttacctga accattttca ccgacaatcg cgattttttt acctaaagaa aactgaaaat
    16321 caccatttac cagtaatgtt ctcccacttc tttccaattg gacattttgt cccatgatgg
    16381 gatagcggct gtggagttca agagattttg gtactggaaa acggatttgt ttcatctgct
    16441 gcggcttggt tgtttctcct attttttgga gtcttctctc taatgttttc gcattctttt
    16501 ggatggcttt ttgtacactg tctttttgtt tagaggaaga caaccgatcc agtctgattt
    16561 gttgcttttt ctttttactg gacacttttt ccaacttttc cgcttgtttt ttcttttctt
    16621 ggatcgattt ttccaatcgt tttttctctt tcatgatcga ctctgcttct cgtagctgag
    16681 tctttttctc caattccttt tgagtgagat aggcactata attccccgaa aactctcgga
    16741 tttctccgtc ttttacttcc cagattttcg atgctagctc atctagaaaa tagcgatcat
    16801 gactaacaaa aaccagcgta ccataataat aacgaagctc ttcaatcagt cgatcaatac
    16861 tttttctatc tagatgggtt gttggctcgt ctagaagcat cccttgttca taagaagaaa
    16921 gaagacgtat caactcgatc tttcgttctt cccctccgct ccatttccgc tctgaagttt
    16981 cttccagtct taactggaaa tgactggcta attccaaatt taggtccttt gcttcagcga
    17041 gataggttaa ttgaggcagg tagttgaagg tgatctctgt ttggattttc ccatgatcgg
    17101 gaaaaagttc accggctatt agtttgagca aagtcgattt accgacacca ttttctccta
    17161 tgatccctat acgatcttgc tgatagacac gaagagaatc aatagatagt tctagctggt
    17221 tgccaaagct gacttgtaga tttgttatat ttactgctaa attttccata tgttttacac
    17281 tcctatttca tgtaaaacaa caattatcga tactaaaaaa gagcagaaaa atctgtttgc
    17341 aaaaaaactg catcacatct tttcctactc tgaacgaaat aactatgact aaatcaccaa
    17401 atctgtcata gctttcacct attctactgt cgttcgaaaa aatggacata ctccatccat
    17461 gaaattgttg tttatacatg tcttttgaat ttatttagtt gttattcaaa agttcgaaac
    17521 gtaatttcat cgatgcagtc atttttccga cctccttttt tattaggtta ccacacacga
    17581 ttcaaaaaaa gcaatagcta ctgaatagta actgaaaacc ttttattcga acatagtgat
    17641 cttttctggt gctaaacgaa cagactcgta accttcttct accgcttttc ctattgataa
    17701 aataataact ggaacataac ggtctttatc caaaccaaat gcctctgcca actgatctgc
    17761 ttcgaatcca ccaattggat ttgtttcata gccatgatca cgcgcaacta acatgaattg
    17821 catagccgct aagctagaat cgactttgac tacatcgttc atttcttctc ttgaaaaact
    17881 cttgtaataa ggaatgatcg cagctaattg ttgatctctg acttcttttg gcatcttccc
    17941 tgattcgtaa gcttggttgt agatttcttc gccatattcg taacattgca tatcgccaaa
    18001 aatcatgacc attgcagaag aagtatcatt ttgtctcgta ttgaaacgga tcaaaggacg
    18061 caagatttct tttgcttcct cgctttcagc gaccacaaag cgccatggtt tcatattgac
    18121 agaagaaggt gcagtcgtcg cttctttgat catttccagc atttcttcgt gtgggatttt
    18181 gtatgtttca tcatatacgc gaacggatct tctaccagtc ataattgttg aaaaatcatt
    18241 tttttgttcc attattatta acctctttcg tttctgttgt tatattgcaa agttgggtat
    18301 tcaatttatc taaaagtgtt agaaattgtt tcatttctac atcgctgaaa gccggggata
    18361 gcacggaacc tgtatcttta tggtccattt cacaatgctt gattttcttc tgggcttttt
    18421 ctgttagcgc tacatacact tcccggttgt tctgaggatt tctctgacgc gaaacatagc
    18481 catttgcttc caataccttc agatgacgag taactgctcc ttgatcaatc tgcaactttt
    18541 cttgcaaact gctttgtagc aatggatttt cttctttcag tctcatcatg atctcgtatc
    18601 tagtaagact tataccgata ttttcttcaa agatctggac gattttttga tcggctaatt
    18661 tgatttgata taaaaggtga ctaatctctt ctaaagacat aatttcctcc tttctaataa
    18721 ttgactcgtc aacgattgac ttgtcaataa tagaataaaa aaagaaaaaa gtcaaagaat
    18781 cagctgtgta ataaaaaaaa cgctctcaat agaaaagtat agagaaaaga aattttttgt
    18841 agaatgaacg gaaaaaatga taaagaaagc acaacgctaa aaccaagatt attgattctt
    18901 atcaaactat agaagatagc tcttctgcag tagataattt tacaccgcca aaaatttccg
    18961 aaatcgcctt ttgcaagaat cccgtcgaat attagaaatt ggatcagtat gcttaaacag
    19021 taataaaaaa gttatgacaa gatgttgtca caaccttttt tgagtaaacg gtgttcaaaa
    19081 acttgcatct gcggtaataa gttcaaccaa acaaaaaaca tgaaaatctg tttttgtttg
    19141 gctgttctta ctacttgatg cagggcagct ttttcactac ctctttttaa taaccataaa
    19201 atcctattcc ttcatatctc cagcccatcg gtgttagcca atctctttcc ttagcactca
    19261 aggtatagtg atgggtacca gttttttcat ttggattaaa tagtcgataa attggcttgg
    19321 aaccaccaga ataaaaactt agaccttcat aacgccaacc gatttttgtt aaagagttga
    19381 tctcagattg tgccattgtg taatgatggt ctccactgta aggattgtat aaacggtaga
    19441 caggttgtcc gctgtttggt gcttgccaag caatcccttc atattcccag cctacagttg
    19501 gcagataatt tttttctccg gcattcaatg tatataaatg ttctcctgta tgacggttat
    19561 acagacgata catcggagaa gttgaagttg gtgctgtatc atactgtgtc agattatagg
    19621 tttcgattac attgttcaat gtagaaccat aattaggcgc agtggcataa cgacctgtta
    19681 gccaagccgt agcatctttg taagagttcg tattgctttt ccatgcaccg gaatagtagt
    19741 aaacgcctgg ctgaaaagaa gtcgtcttca ataccgttac gttatcttgc aatgattctt
    19801 tgtacgaagg atattttcta aaagcggcat tcaccgtaat ccattgccca ttcacatatt
    19861 ccgaagtagg catcgtaaca gattggccat tatagcttcc tttaatccca aatagattgt
    19921 aattaggggc agaagccaaa gtactgttcc cccagccact ttcaacgatg gcttgagcaa
    19981 tcatgactga tgcatataag tcattggcac ttgccaaact ttgtgcagtt gaggcaatac
    20041 tatcaatgaa cgcttgttga gaattagcag atgagcgagc ataaaaagca tagccagtat
    20101 agttttcgaa ttcagacaaa tctgcttgat gggtatgtgt caatgtttgc ccatctgtca
    20161 attggttcgt gatttcttct gcattctctg gattgttctg agcttctgct gatgtactgt
    20221 cttcggtttg tgtttgttct gttgtttcag ttgttggttc ttcggttgaa attgtatttt
    20281 ctgagacagt agctgactgc aaagtacttt ctgtatcgtt ttcaattcct gtttctgaag
    20341 aactcgatgt ctgagttgcg acagtttctg tcgattcttc agctagcact gttgtgttgg
    20401 caactgctag atttcctaaa agaataacgc taaataataa attctttgct ttcatctttt
    20461 ttctcctttg aattatgaaa cttttcctaa ataacagtgt aactaaaaaa gtaaaagcaa
    20521 gtcaatgtcc atatttaaat aacatttgtt tctttttaat aaatttgctt tttctgcata
    20581 aaaagacgta aatgaagaat attcttctcc attcacgcct taaattgatg gtcgtttgaa
    20641 taactattta acaagtaatt ctctctggat attatccgtt atggattata cttcctgtgt
    20701 cgtcggacgg ataatcatgt tgctgacaga catccggtct ggcgtttcta tcgcaaatgc
    20761 tacagcagaa gctatatctt cagatgttaa tccccattct ttttgcgctt catgaagttc
    20821 aagagcagcc tttttgtcgc tgatggtttg atataattca gtttgaacag ctcctggaga
    20881 gatgatcgta gatttgatgt tattttgccg ttgctcttga cgtaatcctt ccatgattgc
    20941 acggacagca aattttgttc cgcaataaac ggcggaatca ggataaacaa catgtcccgc
    21001 cacagaatca gttgaaatga tatgcccaga tttttgtttt tccatgattg gaagtacggc
    21061 ggaaattcca tttaacacac ccatgatgtt gatatcaagc atattttgcc attcatcacg
    21121 gtgaccttca actaatggag cggttggcat gattcctgca ttattgtaca atacatcgat
    21181 tcgaccgaac ttttccatcg ctaaatcgat aactgcttgg acttgtgcaa aatctcttac
    21241 atctgctgtt tgaatatata gctcagcttc tggtaaagat tcttttattg cttttaggcg
    21301 atcttctcgg cgtgcagcta taactagttt agcccctttt tctgctaata gcttcgttgt
    21361 tgcttcaccg attccgcttg aagcgcccat gatgacaatc actttttctg ataaatcatt
    21421 cattctattc tcctcctgtt ttgaaaaatc tcttgcttat actcactatc tcttatttca
    21481 tcttactgtt caaataatcc gttcgacagg tttgacgatt ttggcaggga ttcctgcaac
    21541 gatggtattg tctgggacat ctttcgtaac agttgcattc gctgcaacaa tggcattctc
    21601 gccaatcgtt acgcctggca gaatagttgc tcctgctccg atccaagcat ttttcttgat
    21661 gtggattggc tttactgaca agcctcttcg ttcttttgga tcggttagat ggttcaccgt
    21721 gatgaggttg acacgaggac caatcaatac tgaatcttct aaacagatgc cacctaagtc
    21781 aacgaacatc acttgacgat tgataaagac atttttccct atactaatat gtttgccgaa
    21841 atctgtataa aaaggcagca gaacagtcac cgtatcatct actttgtcac taatgatatt
    21901 ttctagccgt ttgcggatat ctgctggttc atggtaccca gtattcagcg ctgctaaagg
    21961 ctcttcgttt tccgcttgga tctgatggat cacttcaaag agcggatccg ttactcgaat
    22021 ctctttattg acgatttgtt tttcgatgag gcttgtcatt ttgctccctc cttttatcct
    22081 tactttataa cggacggctt atgatgtcta atacttataa aacatactta actattcctt
    22141 ttaactatac ttagtggtat gcttttttaa aaacaggagg gaaaaaatgg atattcgtat
    22201 actgacgtat tttttagcag tagctagaga aaaaaatatc agccgagcag caaaatctct
    22261 gcatatcacg caacctactc tctcaaaaca actgaaagat ttagaagaag aacttggtgt
    22321 gacacttttc actcgaggct cccgagagat acagttgacg gattctggtc gatatcttta
    22381 ttcaaaagga aaagaaatct tgtcgttggt agaaaaaacg gcgaacaatt taacaaatga
    22441 cgcaatcatc tctggagaaa tctacattgg cggaggcgaa actaaagcaa tctctttcat
    22501 ttctgatagc cttcattcgt tgatcaaggc gcatcctgat atccagtttc atttatatag
    22561 cgggaatgca gatgatatca tagacaaact ggatagcgga ttacttgatt ttggactcgt
    22621 aatcgaaccg acaaacaaac aaaaatacca gtcatttcga ctgccagccc aagatcgttg
    22681 gggacttttg atacatcgga atcatccttt agcaaagcaa acagtcatcc atccgcagga
    22741 actgaaaact gttcctttga tcatttcccg gcaaacgtca gtggacagtc aactagctga
    22801 atggctaggt ttcagcatag atcagttatc gatcgtcgga acatataact tattgtacaa
    22861 tgcttctttg atggtcgata acgggacttt tggtgcatta gctctagatg ggatcatcaa
    22921 tacttatggg acggaaaaag tattcatccc actcgatccg ccattacaag cttcactgaa
    22981 tctcatttgg aaaaaagacc agcctctttc tcctgctgct cgtaaatttt tagatgtcct
    23041 aacggagaaa tacagttaag tagagttttt ttccttcgct ttcagctttt taacaacgaa
    23101 taagttaagc aaaagatcca atgattaaat aacataagtt tcttaatttg ttccttcctt
    23161 ttatttctga taattccagt aaattactat aatt
//
LOCUS       DAJSKS010000034        23063 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJSKS010000034 DAJSKS010000000
VERSION     DAJSKS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.035"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(203..658)
                     /locus_tag="ON396_001119"
     CDS             complement(203..658)
                     /locus_tag="ON396_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113220.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(812..2020)
                     /locus_tag="ON396_001120"
     CDS             complement(812..2020)
                     /locus_tag="ON396_001120"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCR3113221.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2132..2464)
                     /locus_tag="ON396_001121"
     CDS             complement(2132..2464)
                     /locus_tag="ON396_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCR3113222.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2612..3487
                     /locus_tag="ON396_001122"
     CDS             2612..3487
                     /locus_tag="ON396_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3113223.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3569..4150)
                     /locus_tag="ON396_001123"
     CDS             complement(3569..4150)
                     /locus_tag="ON396_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCR3113224.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4156..5415)
                     /locus_tag="ON396_001124"
     CDS             complement(4156..5415)
                     /locus_tag="ON396_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR3113225.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5417..6463)
                     /locus_tag="ON396_001125"
     CDS             complement(5417..6463)
                     /locus_tag="ON396_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113226.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6698..6973)
                     /locus_tag="ON396_001126"
     CDS             complement(6698..6973)
                     /locus_tag="ON396_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCR3113227.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7216..8526)
                     /gene="der"
                     /locus_tag="ON396_001127"
     CDS             complement(7216..8526)
                     /gene="der"
                     /locus_tag="ON396_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR3113228.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKCGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8708..9921)
                     /gene="rpsA"
                     /locus_tag="ON396_001128"
                     /pseudo
     CDS             complement(8708..9921)
                     /gene="rpsA"
                     /locus_tag="ON396_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
     gene            complement(10056..10736)
                     /locus_tag="ON396_001129"
     CDS             complement(10056..10736)
                     /locus_tag="ON396_001129"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR3113229.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10785..11420)
                     /locus_tag="ON396_001130"
     CDS             complement(10785..11420)
                     /locus_tag="ON396_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3113230.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11486..12889)
                     /locus_tag="ON396_001131"
     CDS             complement(11486..12889)
                     /locus_tag="ON396_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR3113231.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12876..13907)
                     /locus_tag="ON396_001132"
     CDS             complement(12876..13907)
                     /locus_tag="ON396_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3113232.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13938..14159
                     /locus_tag="ON396_001133"
     CDS             13938..14159
                     /locus_tag="ON396_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCR3113233.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14324..15061)
                     /locus_tag="ON396_001134"
     CDS             complement(14324..15061)
                     /locus_tag="ON396_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HCR3113234.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15214..16554)
                     /locus_tag="ON396_001135"
     CDS             complement(15214..16554)
                     /locus_tag="ON396_001135"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HCR3113235.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16791..18314)
                     /locus_tag="ON396_001136"
     CDS             complement(16791..18314)
                     /locus_tag="ON396_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCR3113236.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18692..19303)
                     /locus_tag="ON396_001137"
     CDS             complement(18692..19303)
                     /locus_tag="ON396_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3113237.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19426..19551)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19633..20349)
                     /locus_tag="ON396_001138"
     CDS             complement(19633..20349)
                     /locus_tag="ON396_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCR3113238.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20355..20924)
                     /gene="scpB"
                     /locus_tag="ON396_001139"
     CDS             complement(20355..20924)
                     /gene="scpB"
                     /locus_tag="ON396_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCR3113239.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20908..21705)
                     /locus_tag="ON396_001140"
     CDS             complement(20908..21705)
                     /locus_tag="ON396_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCR3113240.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21675..22046)
                     /locus_tag="ON396_001141"
     CDS             complement(21675..22046)
                     /locus_tag="ON396_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR3113241.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(22065..22952)
                     /gene="xerD"
                     /locus_tag="ON396_001142"
     CDS             complement(22065..22952)
                     /gene="xerD"
                     /locus_tag="ON396_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR3113242.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6474 a         4927 c         3914 g         7748 t
ORIGIN      
        1 gtaagataac aaaaaatctg agccagaatg aggttcattc tgacccagat ttgattttgt
       61 acaacccatc agtccttatt gacaaagagc ccaaaaatct tgtcacagtc ccttattctg
      121 aataaacggt gtggaagaga cagcctatca gtagtaataa gacacatctc tttcacagcc
      181 tcatttttgc gtaaaaatcg atttattcga aaaattcccc gtatacatct tctacacgtt
      241 gatctggatt gatcacgata tacaagttac ctgttttttc gctgattttt gttgattgat
      301 attgattgtc taatccttct gttcctttat ctagatatgg gaagtacaca agatcaggtg
      361 tgccatttcg gtaaaggatt tccattcgct cgatatcttc gtatttcaat gcgcgattaa
      421 acatgcccat ttccagtccg cctaggttga tatcatgtgt cgcaactgcg tccccttcat
      481 ggaagatttc gattttaaat ccagcacacg gatggatttc aacaaattca ctgccattga
      541 ttcgtccaaa acttgttgtg atttgtttga tccaaagatc gccgatatga cggcggttga
      601 tcgtccaggt ttctccattt ctaaaataaa attttagcgc tgtcatttct ttactcatta
      661 aaaagactcc tttatgatgt aatttattct tgtactacct cagtttagca caaatttatg
      721 taagtggtca catcttatgt tttttcttct aatgacgtat ctgcccactt taagtagcca
      781 acaaagctga gcagatacag tctagtgttt tttaaatcaa tttttctttc gataaaaaat
      841 ccaaaacctg ttttttatca ttttttattc tttgttctac gacggctttt tcagctaatt
      901 gcaaagcttc actaacccac ttaccaggtt tttttccagt ttcttctagc aagattttac
      961 catctactgc taattcgctt aatgacttga taggtaaacg atcataggat tctcctacgg
     1021 catccatctg aggtgtttcg tcaaaataga tcatcaattc ttctacttgc agtgcatgtt
     1081 ttttgcctaa gaagtacaat tcgaatggtt ccaaagctcc agtttctcgt tgtcgtagtc
     1141 cttggacaag tgcttgaacg tctttaatca tttggttaga acacttccac gatttcaaaa
     1201 aggaacgaat ctcattttct tttagatcca gttggctgat caataatgcc catgcttggc
     1261 tctcttcttt caatgggacg gatggtaatt cagcaaaacg taaaagtgct tcgccttctt
     1321 ctttcaaacc gggacagcaa atgtagcact ctgtttcaat aaatgcatgg attcccgcgg
     1381 cccgttcatt tcccaagagc atcttgatga attccacatt tatacgttct acagatattt
     1441 tacctaatag ctcgtggttt tctttgatgg cgtagaaggt ttcttcctct aatttgaacc
     1501 ctagttgact gacaaaacgc aacccgcgca tcattcgtaa ggcatcttca tggaaccgct
     1561 catgaggatt gcctacagct cgcaagatct gttcttctag atcttgcaga ccatcgaaca
     1621 aatcgatgat ttctccatct tctttcaaag caaacgcatt gatcgtaaaa tctcttcttt
     1681 tcaaatcttc tgccaacgaa cgaacgaatt cgacatgatc tggtctacgg tagtcctgat
     1741 aagttgattc cgtccgaaat gtggtgatct catattgttc atcattttct aataccaaca
     1801 cagttccatg ttcgatccca atatctatcg tttttggaaa aagctgtttg atttcttctg
     1861 gaaatgcgct tgttgcgata tccacatcat ggattggctg gttcagcaag atatcacgaa
     1921 cacttccacc tacaaaataa gcttcatatc ctgcattctc taattttttc agaactggaa
     1981 ttgccttttg gtattccaga ggtaattgtt ttaatttcat ttttccaacc tttcctgcgt
     2041 gtttcttgtc ctgagataaa gactcagttg catggtgact gtttgacaag tccgtttcag
     2101 agtcttcaag gccaggacca cttcatcttg tttactcgtc cttacgctca aagcgattat
     2161 ggtctctttg attgaatttt tccatgtttt tttcaaaaac ttcagttaag tcgatattca
     2221 atgaattagc catgatcatc gtaacgaata aaacatcacc aagttcttct gctaccgagt
     2281 taactggttc agaagccttt ttgctttttt ctccataagt gtggttgact tctcttgcca
     2341 attcacctac ttcttcagtt aatcgagcca tctgagctaa tggagagaaa taccccacct
     2401 tgaattgctg gatatattca tccacttcct gctgcatact gtataaagaa cgctttttat
     2461 ccatcttttt ccctccaaaa ctatcttacc aaaactttaa cgcaaatcct agataagagc
     2521 aattgagaaa atcgaatttc catgctacat ttaggaagga atttctaaat ggaaattctg
     2581 tgaaaataca taattgaaag ctggaattca tatggtcaaa cgaaaaacag taaaagaagt
     2641 tctttttatc attataggaa caagtattta tgcatttggt cttgtttact tgaacatcgc
     2701 taatcattta gcagaagggg gagtctcagg gatcaccttg atcttaagag ctttatttgg
     2761 tattgatcca gcttatacga ctttattaat caacatccca ttgattttac taggaggaaa
     2821 gatcctcggt aaacgttcct tggcctatac tgttctaggt acactttctt tgtcattctt
     2881 tttatggatc tggcaaaggg ttccactaga aatcaattta cagcatgatc tactgatcgt
     2941 ttctctactt gcgggtttag ttgcaggtgt aggaagcggg atcgtctacc gcatgggtgg
     3001 aacaacaggc ggcagtgata ttatcgctcg gattttagaa caaaattacg gcatcactat
     3061 ggggcgttcc ctattggcat ttgatatcat cgtcttactt gcttcattaa cctatatcga
     3121 cttgaaacgt atgatgtata cgttgattgt ttcgtatgtt ttcagtcgtg tgattgattc
     3181 gatcttagat ggcggatatt ctgcaaaagg tctacttgtc gtctccaaca aaagcgaaga
     3241 gattgcacca ctactgatga ccggtctgca aagaggtgtg actttccttt ctggagaagg
     3301 cgcattctct ggtaattcaa aaaaaatcat ctatatggtc gttagctcta gtgaattaac
     3361 tgaagctaag aggatcgtcc acgagattga cgaaaaagct ttcctttcaa tcatcaatgt
     3421 gcatgaagtg gaagggcaag gctttactta tttgaagcca cagacaagat tactgaaaaa
     3481 actataagaa aaaagacaaa gataggctat gaggaaagat tccacatcca gctgaccttt
     3541 gtctttttta gtactatact atttttaact aattgttatg ttcacttacc cggttacttt
     3601 tcttttgtgt ctgcaaaagg atctttcggt taacttcatc agataatttc aaaaaagcat
     3661 ccctatctcc atcttctagt gcttgatcga tttgagcata aagcattttt agttttgcct
     3721 cttcttcttt ttcttgaaaa tatcgttcaa ttaattcagt atcttcttca ctgactgaag
     3781 aatcccaaga agaaaatgga ttgtcttcta agatagatag atattctctt gtctgccaag
     3841 cattttcgaa tatacattcc acatataaag gctttttcca attcaaacgg atttcatgaa
     3901 aaatctggtc tgtgtccgta aattcttttc cctgcaaaaa caatttcatg ggttcccctt
     3961 caacagatag atcactgatc tgcagtcctc gatccgcaaa attcgcccct tcaacaaagt
     4021 ggacattgct taaaatcgct tcatgattgg caagataatt caagatccaa aaaacttctc
     4081 ttcggctaaa agatacatga ttgaccatcc acgttaaaaa attcttcttt tcccgtacat
     4141 caattagcat ttttctcacc tctctgttag gtcatctaat aatgacagtg cctccatatc
     4201 tcctggttca agtgttaaat aataagtcaa taattcttgc gctcttttca gctgcccttc
     4261 ttctcttaag aaaaaggcat actctttcaa gaaatctggt tcatgtgata gttcatgata
     4321 agcctgttca tagtgtacag ctgcaacggt aaagtcttct aattcattat aagcatgggc
     4381 taggttccat tctgcataag gattagcagt ttcttccatc tgattgatca cttgtatcac
     4441 atcttcatat ctttcatcat ccaagtaaag attacttaaa gtaagtaatg tttcatcttg
     4501 tttgtctcct acttctaaag ctttcagtaa aagttcttct gctcgctgtc tatcatgcag
     4561 acggaaagca ttttccgagg ccaggtgata aagttcgact tggtaaggat tttctttgat
     4621 tccttcttct aacactgttt gtgcttcttc aatcatttct tcttcttgaa gagcttgtcc
     4681 tagatataga tataacgatt gataatgcgg attaacttct cgaagttctt ccagatagtt
     4741 gattgcctgc tgattttctt tcaattgaag attcgtaaaa gctagctgga acagtcgatc
     4801 atctgtcctt tcttctttca gtgcttcttc taaaggtacg atagctgctt cgaattctcc
     4861 ttgcatactt aaggattgtc ctaaacgttc ctgcatcgag attcctgaaa tttcatttat
     4921 cccgcttgcc aacaatgttt cataaaccac tgctgcctct ttgaaacgat caactgaaaa
     4981 atacaattca gctagcgcaa actggatcaa tggttcatca ggaagcaacc cagctgcttc
     5041 tttcaatttt gcttcactca cttcagggat tccgataact tggtacaaat cagctatcgc
     5101 caataagctt tgcggatagt aatcactttg cttatccact ttttccaggt attcaaaagc
     5161 taagtcgatc tcattatctt cgattgcaat ttctgctaac gggatgttca gtccgtcatt
     5221 ttctgggtat tggttgatca gttgttcaaa aatctgtttt gcttcgatca aaaagccaat
     5281 agccagcaat tcttcgccta gttcagctaa tatatctgct gggtcttctt ttaatgcttt
     5341 ttctaaaaag atttgtgctt gtgacaagtc ttcatctgat aaagcttgca acattttttc
     5401 gctatttgta ttcatttcaa ttccctctta tctcactgac gatttgttga aataccgtca
     5461 ttgttctcaa taattctttt tcgtttggat gttggtatcc gtctattata cctatttttt
     5521 ctaaaacaag tatttttttg cttttttctg ccaaaaaaag aactttttcc aaatactcca
     5581 tctttgaaat ctgttccagc cagtggattg gatagccaag caagtccagc cacacaaaaa
     5641 agttttttag ttggaaatca aaaggatatg cttggagatt ccacacgaaa tgaaacatca
     5701 ttcctaaaaa ttttttcata ctagcagaaa gtagatggcc attttcggtc agataaaaag
     5761 cttgttcaaa cagtttacca taatttgaca ggttttctgc ctctaattga taaaaataga
     5821 tcatttcttc gatcatcccg gcaaacggcc gagtcgttaa ttgcttttta tttggatagt
     5881 ttttatacag attttgcaaa aaacgatgat cacaaatcat tccgcaacag ataaaagtca
     5941 aaaagtcaat ctgctttccg tccacctgcc gctcgctgat tgtttgatca taaacgatgc
     6001 gttctggcag attgatggtt tgcagcacta cacgatttgg tagctgcaaa atcgtccgct
     6061 gcggtgttaa tgctcggctt agagaacgga tcgatacagg gatcatccat agtgatgaat
     6121 caagggttgt atgtttttga aaaaaacctg ccagatccat gatcccttca tttccaaatc
     6181 cgatgataaa catggattga ttttcgggat agcgtttgac gaaatcaagc aggttcttta
     6241 attctgttaa atgattgcat tgagtgttgg cacaaatata ccagtcattg tttgcttgat
     6301 ttgcaaataa cgggttgatc ttatctgcaa acaaatcata atagcgctga ttcgttaaaa
     6361 ataagattgg ctgatcaatg gaaagttctc ttagctgact gactagcgtt tctccgtaaa
     6421 acactttcgt caacgcatct tctacttgat aatcaagttc catacgcatc cctcctcttc
     6481 ttatttttat tttaccatta gtaagcaaat tctagaaaag ataagagcaa agctcacctc
     6541 ataacttgga aataaataaa aattaaataa aatacatgtg ttttctatgt atgacatacg
     6601 gattcggaat ctctgtctct tatttattcc ttttctgcta ggaaataaaa agaaaagcac
     6661 ttgtagccct agagccacaa gcgctttttc aactgtctta tttaacagcg tcttttaatg
     6721 ctttacctgg tttgaatgca ggtactttgc tagctggaat ttcgatttct tcaccagttt
     6781 gtgggttacg acctttgcga gctgcgcgtt cgcgaacttc aaagttacca aaaccgatta
     6841 attgaacttt ttcgccgtta gctaaagcat cttgaatagt tgaaaataca gcatctactg
     6901 ctgcagttgc atctttttta gttaaatcag tagctgaagc aactttttcg attaattctg
     6961 ctttgtttgc catggataga ttcacctcct ccacaagaga tatgatagaa atatcattta
     7021 gttaaaactt tctgagtggt ccagaagttt aaaaagaaat attgattaac ttcaatattc
     7081 tgttatcacg atatcatatg aaccgcttaa tagcaaggtt tttaccaatt ttcacatgct
     7141 ttttttagtc atagaaacgt aacaaacgaa actgtctttt tcttttgaaa aaaaaacgtc
     7201 gatatggtaa aatgcctatt ttctcctacg agggataatt cggatggacg tcccttcaaa
     7261 agtaaatgct tttctgattt gattttctaa gaaacgttca taagaaaaat gcatcaattc
     7321 ctcttcattg acaaaaacga caaatgtagg tggtttgaca gcgacttgtg tagcataaaa
     7381 aatcttcagg cgttttcctt tatctgttgg tgttggattg attgctacag catccataat
     7441 gatatcattc aacacagccg aagagatacg aagattctgg ttcatactta cttgttcgat
     7501 catttcaggc agacgttcca accgttgttt agtcactgca gaaacaaaaa cgattggagc
     7561 gtaatcaaga taccggaatt catcacgaat ctcttgttcg aaatcacgca ttgtattggt
     7621 ttctttcttt acaagatccc atttattcac gacgataata attccacgac cagcttcatg
     7681 ggcgtaaccc gctactcgct tgtcttgttc ccgaatccct tcttcagcat tcagtaccat
     7741 caaaacgata tcagaacggt caatggcacg cattgcacgc atcacgctgt atttttcagt
     7801 attttcgtat acttttccgc atttacgcat accagctgta tctatcatta agaatttctg
     7861 accactttca gaaacgaagt gggtgtcgat tgcgtctcgg gtcgttcctt caatatccga
     7921 aacaattaca cgttcttctc ctaaaatcgc attgatcaaa gaggatttac caacatttgg
     7981 gcgaccaatc aaactaaact taatcgtgtc gtcgtcctct tcttcagtct cttcagagaa
     8041 atacttcact gcttcatcta aaatatcacc aatccctagt ccatggcttc ctgaaacagg
     8101 ataaggatcg cctagcccta aggaataaaa ttcatagata tcgtttctca tttctggatt
     8161 atctacttta ttgactgcta aaatcactgg tttattactt ctgtataaaa ttttcgcaac
     8221 catctcatct gcgtctgtca cgccttcgcg tccgctgact acacaaatga ttacatctgc
     8281 ttcttcaatc gcgatttctg cttgatgctt gatctgatcc ataaaaggtt catcgccaag
     8341 atcaattccg cctgtgtcga tcacgctgaa ttctcgacct aaccattcac cttttgcata
     8401 aatacgatca cgagttactc caggagtatc ttcgacaatc gagatacgct caccagcgat
     8461 gcggttaaaa atcgtagact taccgacgtt cgggcggcca acgattgcaa ttgttggatt
     8521 tgccatatta tttcctcctc acaagtttcc aatctcactt agtttaacga atgcattgcc
     8581 aactttcaag catagattgt caattaaacg aagtattttt attccttatt gaaatactaa
     8641 tcgcttttca aaagaaaaac tgagacaaaa gcagctgctt tcatctcagt cacagataca
     8701 aaatcaatta tttttcagaa tcatcggttg ctttttcttc tacttcttct ttcaacgcat
     8761 cacctaaaat gtcgtatttt cttctggtaa ttcataagac tcaacatttt ttggttcttc
     8821 ttctgaagct ggtttttctt ctaaagcttt gatactcaat gcaatacgat gttcttctgg
     8881 atggatctct aatactttca ctttcacttc gtcaccttca tgaagcactt catgtggtgt
     8941 cgcaatatgt ttatgtgaaa tttgagagat atggactagc ccttctactc cagggaacac
     9001 ttcaacaaag gcaccgaagc ttgtcaaacg tttaactgtt ccgtctaata ctgtgccaac
     9061 tggagctttt gtatcgattt ctgaccaagg tccaggctgt gtgtctttga ttgacaagga
     9121 aacacgttct tgttcaggat tgatagacaa cactttgaca tttacatcat cattgacagc
     9181 caatacatca cttggttttg ctacatgact atgtgaaatc tctgagacat ggactagacc
     9241 gtcaattccg cctaggtcga caaatgcgcc aaaatcagta agacgtgcaa cacgtccact
     9301 gagaacatct ccctcatgga tagttgaaag aatttctttt ttcttttctt ctctttcgtt
     9361 ttccactacc gctttatgcg ataagatcaa acgattttct gatggttcga tttcgatgat
     9421 tttaaagctc aatgttttgc ctttatagtc tgaaaagtct gcaacaaaat gatcttccac
     9481 cattgatgca gggacaaaac cacgtacacc gacgtcaacg actaaaccgc ctttgacaac
     9541 atttgtaaca ggtgcttcga tgaccttgcc ctcttggaaa tctctttcga tatcttccca
     9601 aactttttta gcatctaaac ggcgttttga aagtaggtag ctgccgtttt ctttatcttt
     9661 gccgatcgta ctgataacga ctaaatctaa tacatcaccg acttttacta cttcgctgat
     9721 atcttctacc ggcaaagttg ataattcttt tgccggaacg actccttcaa ctccagcccc
     9781 ttcgattccg acaatcactt gtttgtcttc aatgactaaa acttcgccct taacaacatc
     9841 gccaactttt acttcttgta cgcttttcat tgcgtcttcc attgactggt tgttctcttg
     9901 attttgatca aattctgtca taacacttgt cctcctaacc aacattctgc cgtaagtacg
     9961 gtactgtttt tagtttaatt gattattcaa aaaaaataaa gcgatttcgt tcgttttcct
    10021 tgaaaaaaca attgtttttc attttttttc ttaatttaaa aaagaagata accttttttc
    10081 aaaacaacag cttttattgc tgcaacgact tctggaatcg atttgccagt cgtatcgata
    10141 cgaacagcat cctctgcttg cttcagcgga gagacgtccc ttgttgaatc ataatggtct
    10201 ctttcctgta tctctttcgt cagtgtttca agatcagtcg gaattccttt ttcttggttc
    10261 tctttgaaac gtctttctgc acgctcactt acgcttgcta ctaaaaagat tttcacttct
    10321 gcattcggta atacagctgt cccgatgtct cttccgtcca tgacaacgcc accgcgttcc
    10381 cctatttttc gttggacagc aactaattct tctctgactt ttccatgtgc agagacttca
    10441 gaaacagctt ttgtgacatc tggttgacgg atttctaaag tcacatcttt tgatgctatg
    10501 aatacatgct gtccatcttc ttgttgcgaa aaagagatag gatacttttt gatcagcgaa
    10561 actagccctt cttcatctga aaaagataca ttgttcgtaa tagctaaata agttactgcg
    10621 cgatacatgg caccggtatc tgtataaata taattcaatt cttttgctaa tatcttggca
    10681 acggtacttt tcccagatga agcaggcccg tcgattgcaa tactgatttg tgtcatgata
    10741 taactccttt ttctcagaaa aaaagccgca aagcgcggct attattattt aattcttaat
    10801 tcttgaccag ggtaaagcat aaagttgttt ggatctaagc cattcaattc aaacaattga
    10861 tcagtggtga tccctgcacg ctcagccact tgttttggac cttcgcctgc ttgaacagtc
    10921 gttgttgaac cggctgcagc tgatgaagaa ggtgttgttt ctgctgcatt gctgcttgaa
    10981 tcccctgctt gataagttgc ctcacttgag gaagtcggtg ttgtctctgc tgcaatactg
    11041 ctttctgacg ttacacctgt atcttgagac acagtagatt cttccgcgat tgtactagat
    11101 tctactgttg aagtctcagt tgactcctct tttgttgtac ttgtagaact ttctactact
    11161 gatgtcgaag tttcagaaga agttgctgct gttgcattgt tgcttgatcc atttctgatc
    11221 cagaaataag cacctgctgg aatagcgata cataagacca gtaaaatcaa aaatagcgaa
    11281 agaaataatg tatttccttt cttttgttgt ctctgagaac ttcttgatga aacttcatca
    11341 tctgtatcgt agattggttg ttcccatggt tcttgtgttt cgttattttc tgtagattga
    11401 tgtctatctt ttctgctcac tgttttacct cctaaactct atcgtgtgta ttataccata
    11461 ggaaaaagtg tcttgtccta aaaaatcaat gttccatgtt ttttttaaat aaatttagta
    11521 aaatcgtttc ccaatgaagt tgttcagtct tagaaaaaag cttcctcttt ggcagaagtt
    11581 ctgcttttgc tccatcaata tcacaacaat tctgcggctt ttcggagagt ttttcgccaa
    11641 aataagctaa gatgaatttt ctgcggcagt tttcttcatt aatatagcga agcatctgct
    11701 gcaggcggaa ttctttttct ttttcctgac gtttgagccg ttccacccaa ttttctggtt
    11761 tttcagactg ctggatcagc tccatccatt ttttttgcag ctcatcaaag ggagcttgct
    11821 ttgtggcata ttccaaataa agctcaaaac tttgtcgctg ttctctagaa agttgattaa
    11881 agaaataatg gatacgttca tccccacttt gataaaaaag aattgctgca ctttcttcct
    11941 gatctctgcc tgctcgaccg atttcttgta catagttttc tagactgtct ggcaaatcat
    12001 aatggaccac ataacggata tcagatttat cgatccccat accaaatgca ttcgtagcaa
    12061 tgagaaactg cagctgattt tttacaaact gctgttgaag ttgtcttctt tgagctgcat
    12121 caagaccacc gtgatagtat ccaactgtaa aacgttccct gaataaatga tacaatcgtt
    12181 cgacttcttt ttttgttgca caataaatga ttgccgctcc ttgtgctctt tccatgaagt
    12241 gttcaagttc ttgttctttt tgctgcgttt tccgaacaaa aagcgagata ttttgccgat
    12301 tgactgattg tctgatctct tttggtggtc gatcaaatag tacatgacgt atatctcttg
    12361 ctactaaatc tgttgctgtc gccgttagtg ccagtgtgac cgggtttcct aaaagctttc
    12421 ggatttttcc taactgttga tattctggac ggaaatcaac tccccattga gacacgcaat
    12481 gagcctcatc tactacatat aacgcaatat tttgtttttt cagatgctct aaaagagaag
    12541 gattagtcaa catttcggga ctcaaaaaca aaaatttata ttgcgataga tgacggagga
    12601 cgtatcttct ttcagattgc gaaagtaaac tattgaaagc agctactcgt ttctcccctc
    12661 gtttttgtaa ttgcgtcact tgatcttcca ttagagaaag cagcggtgac acgatgatga
    12721 ccagaccttc cattaaatac cccgtcaatt gatagcaaag gctttttccc gttcctgtag
    12781 gcaaaatagc gagagtatct tctccatcaa gcaacgcctg aataatttct tcttggccgg
    12841 gacgaaatga atcaaatcca aaatattttt ttaattcatt tattaacatt ctgtatcgct
    12901 tcctttctca atagatagat ttgatacaaa cgaaattctc cataatctaa ttgtccatct
    12961 acattccatt cgctaaagcg ccactctcgc acggaatcct tatttggttc caaacgttcg
    13021 attgtttctg cagagagaat ccactcgtat ggaaaatcat caaaaaacaa ggcccactca
    13081 ataagatgat ctgttactgt tcctcttttt aaatgacgga ttgccatgat ctggtcaatt
    13141 gtctgaccat tcataaacat ctgcttggtg atcatcatgc tttgattgaa attttgctgt
    13201 aataatgaat cgatcaaagc gtgccaaagc gaatctggcc gtttttcgat ttgtgctaac
    13261 aaaaggtgga tacatctgct ttgttgcaga taaacctctg actcatcatt gttttttgcc
    13321 agctgataag gaagcagccc tgttcgctca tttccagata attggttagc taaaaaatct
    13381 gctgcttctt ctggtatctc cgaaaacagc tgcaccaaac tctcatacgc agtatcaatt
    13441 aaaatttcac gtggcaattg gctttcacta agccattttt ttagttggaa agtataaaaa
    13501 ggacttgtct ctgctggaag atagtcttga atacccgttg ccagattaga aattacttga
    13561 acagcaaatt taatgagccg ccaactagtt tcccctgttc tgccatagcg gtcaaaacgc
    13621 aaaccagtat gttctatttg atgttcggac aacatatcag cccctgcagg cgttagcttt
    13681 gcttcatttt catttatttc gatccaacct tgctgcgcaa ttttttgcaa aatttgataa
    13741 aacttatcct gcttcaattc tggaaaactg ttatggataa acaaaagttc gtgggtaaat
    13801 ccataaataa gtactgaagt cgttcgtttt cccacaagga gttggtaaag agaactaatc
    13861 cgcagcttgt gtcctgatga aaatagcgat aaaataaaaa caatcatatt attctcctta
    13921 agttaggatg gttagaaatg tcactactat gtaaacttgt acctgaacgc tgtattgcat
    13981 gcggtctttg tcaaatcgaa gcgccagata ttttcgatta tgatgaagaa ggaatcgttt
    14041 tatttgccca agaaccagag gctcatcaac agttcgtgat gccggaagaa gaaacatatg
    14101 taaaaaaagc gtatcaaaaa tgtccagttc gagcgatttt attagaaaat aaaatataaa
    14161 tataaacgaa actttaaaaa cgtacactaa gttctctaaa cttgaaaata agccgggatc
    14221 tgtttctctt gttccgtgaa atcccgactt attttgaaag attcatttgc tatctatagt
    14281 taattgacta gttttttcac ggaccctata ggacccattt atattatttc aactttttca
    14341 gccagttttc caatgtattg acgatcacag tttgctggtc tgcgttagaa atttctgcct
    14401 ctccatctcc tttttgctca ccatagctgc caaaacctcc atgatttccg ccattgattg
    14461 atacatactc tgttctttcc ggtaaatact gcttagcttc ttgataagct tcctgattca
    14521 atactttatc ttttgtcgcc gtgatcgata aaacaggaac agctgattct gtcaatgttc
    14581 cttttttgtc gggataactt gccatgaaga ataccccttt taaatgtttg tctgaatgat
    14641 tcttattgac ataacgactt gccattacgc cacctaatga atggccaccg atcacataat
    14701 cggaatttgg aaaatcttct ttgaccttat ccgctttgtt tccgcccatc actgctagat
    14761 caagtggcat tttgacaata gcgactgaaa agccgttttt tgctaaatct tgcgcccaaa
    14821 tactataact ttctggctct acgagagcac caggataaaa aataagtaat ggagactttc
    14881 catgattctt tttctctcct tcaaaaaata agtaatcttc tttgtttttt ccttctttta
    14941 ccaacacttg cgcttctgac gatggttcgt acgtattttg ctgtatatag taataaccgg
    15001 ctccaccgag taatcctaaa atgatgacta agccaattag tattcgtcta aatattttca
    15061 ctttctccca ccttttctat ctgagtgacc cctttttctt aatcaagtat gattatacct
    15121 taaaaaaata agagagaaat gcaaactttc cagcatcaaa taaaaaaaga cggaatatcc
    15181 atttgaatat ccgccctttt tgtatgttta tttttattta gcacataaac gatacacagc
    15241 ttctgcataa atttccattg ctttatacat actacttaat tcccatcgtt cattcgcctg
    15301 gtgcataaag tcaggagtat cagggaacat cgcaccgaat gccacacact ggttcatcgt
    15361 acgagcaaaa gtagcgccgc ctgatacaaa cggttcagtc atgtctccag taatatcgcg
    15421 atatgtcccc aatagatttt tcactaactc actgtctagt ggcacgtata atgaatcaag
    15481 gaagtcaaat tcttcataag tcaacccata tttttctgct gttttagtta atttagctac
    15541 taaatcatct ttcttgaatg taacagggat tctcatatct acaccgattt ttgtttgttc
    15601 tggtgtgatt tccaaacttg caaagttcat cgttagctcg ccggattgtt catcttctgt
    15661 tttgcccacg acagcttcac cagttgcatt ttcttgaact aattttccta agaaattgat
    15721 tgggccaaaa tcaaatactt ctgaaagagc aattgccaga cgagaaatag cattcactcc
    15781 ttgtgctgca tctttcgcgt ggatactttt accaagaacg acgatgcctt caccttgttc
    15841 ttcgaaatcg aattcgtgtt ttttcaatgc ttcttttact tcagaaagct tttctccaga
    15901 gtaaggagct gcatcaggaa caacattcaa cgctcctcca gcttttacag aaaactcgtt
    15961 cgttcctggt cctgtcaagt aagcttgcaa taagcctttt tctgcataaa ttaaaggaaa
    16021 ttcagcatct ggcgcaaatc cttgcgtaat tccttcttct ttttcgttat atttttctaa
    16081 acaacgccat aaattttctt catctgtacc aaagataaaa cgaatacgtg tcttgaattc
    16141 cacgccagca tcaatcaaag ctttgacggc atacattgcc gcaatagaag gacctttgtc
    16201 atcttgggat ccgcgaccaa ctaaccagcc gtcttttata gttggatcga atggttttgt
    16261 ttcccaatta ttttcgtccc ctgctggaac aacgtccata tgacacaaga taccgaataa
    16321 ttcatctcca gagccaatct ctgaataacc gtaatagcct tctggatctt taaatgttct
    16381 aaatcctagc ttttcactga tttccaatac tttatccaaa gcttcaatca cttttttacc
    16441 gaagggatgt ccttgtcctg aatctgcttc atctagtacg gaaggaattc ggatcaattc
    16501 gctcaatgat tctaatgctt gttcatgatg ttgttctgtc acaaattttt tcatgatatt
    16561 atcctcctaa tatgttaaaa agggtcatgt caaattcctc cgacacaacc tcttcttctc
    16621 aaataatgac gttttaagcc tgctccatca gcaagcagaa tgttcttaac aaatgatgaa
    16681 agaaaaaaca ctcactgaat ggagctattg acttgtccac gtgcttatcg atagccgttt
    16741 atcggtttct tcattcttat gatagacctt gtacctgtat ttgtctaggt ttagaacatt
    16801 gctgctacac ctaggatcac acagatggct acaaataata tcgcaattaa tttaccggta
    16861 aatttccacc acactgaaac atcgaaacga gcaattgcta cagcacccat tacgatagca
    16921 gatgttggtg taattaagtt tactaaacca gatgcagatt gataagcagt gattactaaa
    16981 tccttaccga cgccggcaaa ttcacccata ggtcccatga ttcccattgt tgcagcagct
    17041 aagccggaag ttgaaggaat caagaatgac atagggatat agaaaataaa tgtaataatg
    17101 atgaatacac ttgaagataa tgaacttaat ccttgttcgc cccaatgtaa gatggtgtct
    17161 gtgattaatc cattgttcat aaccacttgg ataccacgag caacagctac tacgattgct
    17221 acaccgatca aatcagaagc accagctaaa aattcagaga caaattcaga ctctttcatg
    17281 ccataagtga ttgctactac gatggacatt actaagaata acatagtaat ttctgggaag
    17341 taccagtcac ctaatggaag catgctttta cctagaaccg tacctagtac cggaaggttt
    17401 gttaaccagt ttgtcaatga ttcaaaaatc gtgaaattcg cattcaaggt tgtccaagga
    17461 atcaaactga tgatcatgat accaaaagta ataaagaaca atacattaac atgtttttgt
    17521 tttttcgtga ttacttcttg acgaccgatt gcttcaatat cgaaatgctt catatcttct
    17581 ttacgttggt tgtatactaa tgattttgtt ggatcttttt cgatctttga tgcataacga
    17641 tacacatata caatagaaat agctaacaaa ataacgaaca tcaagaatcg aagaccaata
    17701 ccttcaccca tcgaaatccc aacagcttgt gaagctacgc ctgttgcaaa aggatttacc
    17761 gttgaagcta gacagcctac ttgagaccct actaatacga ttgctacagc tactactgta
    17821 tcaaagccta cagccatcat gactgggatc agtaaagcat agaatggaat cgtttcttct
    17881 gccattccgt aagtagagcc gcctaatgca aataacagca tcaatactgg aataagcatt
    17941 ttttcttttc ctttgaattt cttcacaact gaagcaatcc cggcatctag tgcccctgtt
    18001 ttgttgataa tacctaaaaa tccaccaaca accaaaatga agaaagagat tgggattgca
    18061 gcaggtgttt ctttagttag ctcccccgct ggtacaccta gcatcccatt gattggcgcc
    18121 atgaaaatat cccacagccc ttgaggattt tgttgtactc gttcatatga tccagcgacg
    18181 atgttgcctg catcgtctac actatagtgc ccagctggta taaaccaagt aagtacagcg
    18241 attactgcaa taataataaa taagaccgta tacgctgaag gcatttgaaa ttttttcttt
    18301 tttgtttgtt ccatcttctt cttcctttct tttccgttag agacaacaat tttggctcta
    18361 atgttatttt atcaaaaaga gtatcagaac aaaaataata acacgcattt tgtaaaactt
    18421 ttgttcaaaa agttcgtttt tccctattcc ctcctctttt ttcaggtttt taaagctttc
    18481 ttctttttca ttgtagcgtt tccttttttt tataaacaaa cattttcgtc ttttaaaagg
    18541 agaagaattt cacatttgca aagaattgat tgatttttat gcataaatca aaaaaatact
    18601 caaaacaaac aaataaaacg tttacattat tgcatgtctt gtattttttt ccaaaaaaaa
    18661 agaccaagaa atttcttgat ctttttgata tttatttgac tgctgtatga cgttgttcga
    18721 actgatgttg ttttcgactt aaccatggaa gaagttgcgc atgcagcact aaaaagacag
    18781 cactcacgat gaatcctttg atcaagttga aaggaacgat cccaatcagt acatagtttg
    18841 ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa aaacattaag taaagaggcg
    18901 taataacgaa ataatttgcg atactcataa aaatagttag agcgatggtt cctgtaatga
    18961 cacctaaaaa tttgtttctt cctttatgct gtttctttac actaaagaaa taataaatcg
    19021 ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc tcctaccaaa ttatccggag
    19081 aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa cgctgttgca actccggccg
    19141 ctggaccgaa taagaacatg ctgatcaata ccgagatatc actaaaatcg atcttcaaaa
    19201 aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa aactaatgcg atcgcagcaa
    19261 acatcgctac tgcaaccatt ttctgtacac gggtgttctt catttgaaat cctcctgtta
    19321 ggactcctct tcagcgaact ttttgacgtt tctctccaaa tgaaaaaact ctattttcca
    19381 gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa ataagctcta tcttctttat
    19441 ccagactata ctgtcggtat cggaatttca ccgattcagc tatttttttg caaaataggt
    19501 cgtggactat aaccaccggt cgggaatttc accctgcccc tgaagacgaa ccttatataa
    19561 tttttgttta catattcatt atacatatct attcaaaaaa cgcaacatct aacacttata
    19621 aaaagtaagt gttcattttg agtggttcag caaactgctg acttcttttt tgttcagttc
    19681 acggtaatcc ccaggacgta ggccttgtaa agtcagatct ccgtattttt cacgttttag
    19741 tttttgcaca ggaagaccaa ctgcttgaag catatttttc acttgatgat tacgtccttc
    19801 gtggatcgtc aattccacga tactgtggtt tgtcttagga tccactgata aaatttggaa
    19861 tttagctgga gatgtttttc gtccttcgat ctttattcct tttgtcaaag gtgcaagcat
    19921 cgattttgta gcgatccctt ttacctttgc aacgtaaact ttatccactt catgttttgg
    19981 atgtgtcagg cgctgagcaa aatctccatc atttgtcagt agcaagatcc ccgaagtatc
    20041 gtaatccagt cgacctacag gatagatacg ttcttttacc atcggcaaaa gatccgtaac
    20101 aacttttctt cctttgtcat ctgcaacagc agaaataact cctttaggtt tgtacaataa
    20161 atagtaaccg tattcttctt gataaattgg gacttcatcc acttccacag tgtcatgttt
    20221 cccaactttt atccctaatt cccttacaac ttgtccgttt acttttacac gtcctgcaag
    20281 gatgaattct tctgcttttc gacgagaagt gatgccagcg tgggcaatta ctttctgcaa
    20341 tctctccatt acaattattc tcctttattc attccatcaa agaatagatc tggtgacacg
    20401 tcttcttcaa gtgaaatttc catttcttgt gtatcaggca gttcttctaa tgatttcaaa
    20461 ccaaaataat ctaagaagta gccagttgtt ccatataata tcggccgacc tggtccttct
    20521 actctacctt tttcttcaat cagtttatgg gcagctagac gctgtaccgc tcctgaaacc
    20581 tgaactcctc gaatctgctc gatttcagct cttgtgattg gctgtttata agcgatgatt
    20641 gctaaggttt cgacggcggc ttgagataat gtgtttgatg tcccttgcgc atatcgctta
    20701 agtaagggag ccagctcttt tttagtcgtt aatacataat ggctttccgt ttcaaaaata
    20761 tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta attctttcac taaacttgtg
    20821 acttctgctt cgctgagatt taacagtccg cttaattctt cattactgat cccttcctcg
    20881 ccaacaataa aaagaagggc ttcaagttca cttaatttcg tcatgttcgg tatctcctac
    20941 tgctggataa agcataattg attcatagtt ttctttttgt tgcgcaatga tctgcccttt
    21001 tttcatcaat tctagcaaag ccataaaagt agtgacaact tcacttttag aaggtgactc
    21061 aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt atacgctctt cgataaatac
    21121 cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt ttttccactt tttgagaaaa
    21181 tttcttacgt tctaagattt tctgcatggc acggatcaaa tcgatttttt tgacttgtcc
    21241 tcgcttcaac ggcttttcag catccgctaa cccatcgatg ttcataggtt cttttgtgta
    21301 ataaagactt cgttcctctg ctttttctgt cagtctttca gctgcatatt tatatttacg
    21361 gtattctaaa agctgagtga ccaattcttc tcgaggatct tcttcccata attcttcatc
    21421 gtctgtgccg aattcttgga caggaagcaa tgtcttgctt ttgattgcca tcaatgtagc
    21481 agccatcact aagtattctc ctgcaacagc tagctcgagt gttttcattg tatggatata
    21541 attcatatat tgctccgtga tcgtagcgat cggtatatca tatatatcga tttccatttg
    21601 ctgaatcaga tgaagcagca gatcgagagg accctcgaat acatctaatt taatattgat
    21661 ctccgtcaac tcattcactc tttcttcttt ttctttgcta gccattttcc aatgtaaggc
    21721 tctgtgattt cagttgcacg gatggagaca tccgggtact tttcctgtaa ttcatctaac
    21781 atttgaaagc ccatattctc accgcgataa gacggattca aagaaatgtc atggatgatc
    21841 agctgatcgt tcgttgccca gtaaatacca ataatccctt gaatgttggt tgaaccttct
    21901 ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt acgtatcaat ctccttcaac
    21961 aaagaagttt tacctgtcag tttcttatgg aaactcaata gtcccatagc aattttacgg
    22021 ttttctttcc ggtaatgtgt taacatcttc tcaccttcct atgactaagc ccttggaaaa
    22081 tgctgcttat aaacatccgt cattcttttc ttcgtgatat gtgtataaat ctgagtcgta
    22141 gagatatccg cgtgacctaa caattcctgt actgtccgca aatcagctcc attttctagc
    22201 agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta cttctttgta aatcccagcc
    22261 tctcgtacta actgcttcag atttttccag attccttgcc gagaaagacc tttcccatga
    22321 ttattgacaa atacatgcat ttcagatgcg tcctttacaa gtaatggacg tgcttcgtct
    22381 aagtagcgtt ccagccattg gatcgcataa tcacctaaag gaatgatccg ctctttatct
    22441 ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa gatctcccaa tttcaatcca
    22501 atcaattcac tgacgcgcat cccagtagca tacatgactt ccaagattgc acgatctctt
    22561 atccctaacg tttttgtcgt atcaggtgct tcaatcagct tttctacttc tgtcagagtc
    22621 aatgtacttg gtaatttctg tgctttcttc ggcgtatcga tatgttgcat cggatcatga
    22681 tcagtgatac gctcttgccg taaaaactga tggaatcttc tgagacttga gatcatccga
    22741 gtaacagttg ctgtcgcttt tttttcttca tgcaacttct ccagaaaacg gatgactaaa
    22801 aagcgatcga catcctgcca tgaagcaatt ttctgctcct gtagaaaatg caagtactgt
    22861 tccaaatcac gttcatagct ttgtcgtgta ttggcggaca atccccgttc aatattcaag
    22921 tagtggagat aatcgttgat ttgctctttc ataataatcc ccctcatgcc tctctatcat
    22981 atcagatacc gcttcattct gatagtcttt ttccataatc tgagcaaaga aaaagccgtc
    23041 tagacactca gacggactgg ttt
//
LOCUS       DAJSKS010000035        22006 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJSKS010000035 DAJSKS010000000
VERSION     DAJSKS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.036"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            34..276
                     /gene="yaaA"
                     /locus_tag="ON396_001143"
     CDS             34..276
                     /gene="yaaA"
                     /locus_tag="ON396_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCR3113243.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            263..1387
                     /gene="recF"
                     /locus_tag="ON396_001144"
     CDS             263..1387
                     /gene="recF"
                     /locus_tag="ON396_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR3113244.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1384..3330
                     /gene="gyrB"
                     /locus_tag="ON396_001145"
     CDS             1384..3330
                     /gene="gyrB"
                     /locus_tag="ON396_001145"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR3113245.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3350..5821
                     /gene="gyrA"
                     /locus_tag="ON396_001146"
     CDS             3350..5821
                     /gene="gyrA"
                     /locus_tag="ON396_001146"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCR3113246.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6057..6356
                     /locus_tag="ON396_001147"
     CDS             6057..6356
                     /locus_tag="ON396_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR3113247.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6406..6918
                     /gene="ssb"
                     /locus_tag="ON396_001148"
     CDS             6406..6918
                     /gene="ssb"
                     /locus_tag="ON396_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3113248.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6944..7180
                     /locus_tag="ON396_001149"
     CDS             6944..7180
                     /locus_tag="ON396_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR3113249.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7323..9296
                     /locus_tag="ON396_001150"
     CDS             7323..9296
                     /locus_tag="ON396_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HCR3113250.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9302..9754
                     /gene="rplI"
                     /locus_tag="ON396_001151"
     CDS             9302..9754
                     /gene="rplI"
                     /locus_tag="ON396_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR3113251.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10028..11395
                     /gene="dnaB"
                     /locus_tag="ON396_001152"
     CDS             10028..11395
                     /gene="dnaB"
                     /locus_tag="ON396_001152"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR3113252.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12175..13467
                     /locus_tag="ON396_001153"
     CDS             12175..13467
                     /locus_tag="ON396_001153"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR3113253.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            14254..15117
                     /locus_tag="ON396_001154"
     CDS             14254..15117
                     /locus_tag="ON396_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR3113254.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(15246..16868)
                     /locus_tag="ON396_001155"
     CDS             complement(15246..16868)
                     /locus_tag="ON396_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3113255.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            17117..17638
                     /locus_tag="ON396_001156"
     CDS             17117..17638
                     /locus_tag="ON396_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCR3113256.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(17824..19554)
                     /gene="spxB"
                     /locus_tag="ON396_001157"
     CDS             complement(17824..19554)
                     /gene="spxB"
                     /locus_tag="ON396_001157"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCR3113257.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            19719..20537
                     /locus_tag="ON396_001158"
     CDS             19719..20537
                     /locus_tag="ON396_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HCR3113258.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(20640..20837)
                     /locus_tag="ON396_001159"
     CDS             complement(20640..20837)
                     /locus_tag="ON396_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113259.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            21006..21584
                     /locus_tag="ON396_001160"
     CDS             21006..21584
                     /locus_tag="ON396_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCR3113260.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            21679..21816
                     /locus_tag="ON396_001161"
     CDS             21679..21816
                     /locus_tag="ON396_001161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113261.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         7050 a         3979 c         4653 g         6324 t
ORIGIN      
        1 tatactttta ataatgaaaa tgagggatgg gcattgaaac aacaagtcat tctaaatacg
       61 gaattcatga cgttgggcca aatgttgaaa gaagtcacag ttatcggtag tggcggacaa
      121 gcaaaatggt atttggcaga aaacactgtg ttagtcgatg gcgaaccaga aaatcgtcgt
      181 ggacgcaaac tttatcccgg catgatggtt gaagttccag aagtaggaac tttttttatg
      241 gtaaaaaaag gaaacgacga taatgaggct gaataagctg tatttaaaaa attatcgaaa
      301 ttacgaagag ctgaacatcg aattttccaa gaacctcgtc atctttttag gagaaaatgc
      361 gcaaggaaaa acgaatcttt tggaaagtat ctacgtatta gctatgacgc gaagtcatcg
      421 gacaagtagt gagcaggaat tgattggctg gactgacgat caggcgatga ttcaaggaga
      481 gatcacaaaa ggttcatcca ctcttccatt agaaatctta ttatctaaaa aaggccgaaa
      541 aaccaagatc aaccatatcg aacaaaaaaa gttaagtact tacgtgggac aaatgaatgt
      601 cattttattt gcaccagaag atctttcttt agtaaaaggc agcccgcaga tcaggcggaa
      661 gtttttagat atggaattag gccagatcaa tccaatctat ctttatgatc ttgtccaata
      721 ccaagcaatt ttgaaacaaa gaaaccagta tttaaagcaa ttgaatgaaa aaaaacaaac
      781 tgatttgctt tatttggata ttttgtccga acaactagca gcgtttggca gcaaagtatt
      841 gaaagcaaga gcacagttcg tcaagcgatt ggagttttgg gctaattctt tgcatcagca
      901 aatcacccat caaaaagagc agctggaaat cgagtatctc accgcagtcg attctttaga
      961 aacacatacg caagagcaaa tccaagaaca atttctcgca cttttgaacc aaaataaaaa
     1021 gaaagacctt tttcggggaa caacgacagt tggtcctcat cgtgacgatc tttccttttt
     1081 tattaatcag aaaaatgtgc agacttatgg atcgcaagga cagcaaagaa caactgcact
     1141 aagtgtcaaa ttagcagaaa tcgatttgat taaagaagag acgggggaat atccaatcct
     1201 tttgttagat gatgtgatga gcgagctgga tgatagtcgt caactccatc tgctagaaac
     1261 aatcgaagga aaagttcaaa cgttcttaac aacaacgaca cttgaccatg taaaaaataa
     1321 aatgactgtt gaacctgaga ttttttatgt tcaacaaggg aaactaaaag gagaagaaac
     1381 tgaatgacag aagaaagaag tttagtagaa cgcgctaaag agtatgatgc cagtcagatt
     1441 caagtacttg aaggacttga agccgttcgt aaacgacctg gcatgtatat cggttcgact
     1501 agttctgagg gtctgcatca ccttgtttgg gaaattgtcg ataattcgat cgatgaagta
     1561 ttagcaggtt ttgcaacgaa aatccatgtg attattgaaa aagacaacag tatcaccgtt
     1621 attgacgacg gtcgggggat acctgtagat atccaggcaa aaactggacg tcctgcagtt
     1681 gaaacggtct ttactgtttt acatgctgga ggaaaattcg gcggtggtgg gtacaaagtt
     1741 tctggtggac ttcacggagt aggatcttct gttgttaacg cgctttctac acagcttgat
     1801 gtcaaagtat acaaagaagg aaatgtgtac tatcaagaat atcgtcgtgg agctgtagtg
     1861 gacgacttaa agattatcga acaaacggat cgccatggaa caacggttca ctttacgcca
     1921 gaccctgaaa tttttacaga gacaactgag tttgattttt caaaattggc tacccggatc
     1981 cgtgaacttg ctttcttgaa ccgcgggatg cgtatatcga ttgaagacaa acgagaagaa
     2041 gaaccagtca tcaatgaata ccattatgac ggcgggatta aaagttatgt cgaatacttg
     2101 aatgcaaaca aaaccgtgat ttttccagaa cctgtatatt tggaaggtga acagcaagat
     2161 atcgcagttg aagtctctat gcaatataca gacggctacc attccaatat catgagtttt
     2221 gccaacaata tccatacgta tgaaggcgga acacatgaat caggattcaa gactgcttta
     2281 actcgtgtga taaatgatta tgcccgaaaa caaaagctga tgaaagaaaa tgacgacaac
     2341 ttgaccggtg aagacgtgcg agaaggattg acagctgtta tttctatcaa gcatcctgac
     2401 cctcaattcg aaggacaaac aaaaacaaaa ttgggcaatt ccgaagtacg gacagtcact
     2461 gatcggttat ttagcgaaca tttcatgaaa ttcttgctgg aaaacccaag tgtgggccgt
     2521 caaattgttg aaaaaggatt actagcttcc agagctcgtc tagcagcgaa acgtgcgcgt
     2581 gaagtaacga gacgtaaagg tgctttagag attagtaatc tgccaggaaa attagcggac
     2641 tgctctagca acgatcctga aaaatgcgaa ttgttcatcg tcgaaggaga ttcggccggc
     2701 ggatcagcta agcaaggtcg taatcgagag tttcaagcca tcttgcctat tcgtgggaaa
     2761 atcttgaatg ttgaaaaagc aagcatggac aagatcttgg caaatgaaga gatccggtct
     2821 ttatttactg ctatgggtac agggtttgga gaagattttg atgtatcgaa agctcgctac
     2881 cacaaattag ttatcatgac tgatgccgat gtcgacggcg cgcatatccg tacattgctg
     2941 ctgactcttt tttatcgtta catgcgtcct attgttgaag ctggatacgt ctatattgct
     3001 cagccacctt tatacggggt aaaacaaggg aagaatatta cgtatgtaca accaggaaaa
     3061 aatgcagaag aagagttgaa acaagtcgtt gcttcacttc ctgcctcacc aaaaccaagc
     3121 gttcagcgtt ataaaggact tggagaaatg gatgatcatc aactatggga aacaactatg
     3181 gacccaagca atcggatgat ggcccgcgtc agtgtggacg atgctattgc agcagatcag
     3241 atatttgaaa tgttgatggg ggatcgagta gaaccccgtc gtgcattcat cgaggaaaat
     3301 gcacactatg tgaagaactt agatatttaa tcatgaagga ggaaaaagaa tgagtgaaga
     3361 aatcagagaa aacatccatg atgtcaatct aaccagcgaa atgaaagact ccttcatcga
     3421 ttatgcgatg agtgttattg tcgcacgtgc acttcctgat gtcagagatg gtctgaaacc
     3481 cgttcatcgc cgtattttat acggcatgaa cgaattaggt gtaacaccgg ataaagccca
     3541 taaaaaatct gccagaatcg tcggagatgt catgggtaaa taccatcctc atggagacta
     3601 tgcgatctat gagtcaatgg tacggatggc acagccattc agctaccgtt atatgctagt
     3661 ggatggtcat ggaaactttg gttctgttga cggtgacggt gccgccgcta tgcgttatac
     3721 cgaagcacgg atgagcaaaa tcgctacaga aatgctgcgt gatatcaata agaacactgt
     3781 tgatttccaa agcaactatg atgatacaga aaaagaacca gttgtactac ctgctcgctt
     3841 cccgaatctt ttagtgaacg gaacaacggg gatcgccgta ggtatggcca cgaatatccc
     3901 acctcataac ttgtctgagg tagtagcggc tatcgatttg ctgatggata atcctgatgt
     3961 gacgaccaat gaattgatgg aagtattacc tggaccagat tttccaacag gtggtttggt
     4021 tatgggaaaa tcaggaatcc gaagagcgta tgaaacaggt aagggttcta ttaccgtacg
     4081 tgcaaaagtt gagattactg agatgccaaa tggtaaagaa cggatcatcg tgacagaatt
     4141 gccttatatg gtcaataaag caaaattgat cgaacggatt tctgaactgc acagagacaa
     4201 gcgtatcgaa gggatcacag atttgcggga cgaatcttcc cgtgaaggta tgcggatcgt
     4261 cattgacatc cgtcgtgacg ccagcgcatc tgttatcttg aataacttat ataaattgac
     4321 atccttgcaa aattcatttg gattcaatat gttggcgatc gaaaaaggtg tgccgaaagt
     4381 tttgagcttg aaacaaattc ttgaaaacta tatcgaacat cagcgcgaag tcatcacaag
     4441 acggacagtc tttgaaaaag aaaaagcaga agcaagggca cacattttag aaggtttgcg
     4501 tattgctttg gatcatatcg atgaaatcat tgcgattatc cgcgggtcta aatcagatga
     4561 cgaagcaaaa gcgacgatga tcgaacggtt cgatttatcc gatcgccagt ctcaagctat
     4621 tttagatatg cgtttacgcc gattgaccgg tttggaacgg gaaaaaatcg aaaatgaata
     4681 ccaagaactc ttaaaactga ttgaagattt aactgacatt ctggcaagac cagaacgtgt
     4741 tacagaaatc atcaagaatg aattagcaga cttgaatcaa cgatttggtg ataaacgacg
     4801 cacagaatta ctagtcggtg aggtcttaag tctggaagat gaagatttaa tcgaagaaga
     4861 agaaatcgtt attacattga caaacaatgg ttacatcaaa cgtttggcaa ataatgaatt
     4921 cagagctcaa cgaaggggtg gacgtggtgt ccaaggaatg ggtatccaca atgacgactt
     4981 cgtcaaaaat ctggtttctt gttctaccca tgatacattg ttattcttta cgaataacgg
     5041 aaaagtgtat cgggcaaaag gatacgaaat tccagaatac gggcgcacag ccaaaggaat
     5101 accaattatc aatttactag gtatcgattc gagtgaaagc atccaggcaa tcattgcggt
     5161 gacgggagaa gcagaagaag gccactatct cttctttacg acgcgtcaag gtacagtaaa
     5221 acgtacttct gtcactgctt ttgctaacat ccgaagtaat ggtctgatcg caattggttt
     5281 gaaagaagac gatgagctcg tcaatgtgct actgacagat ggtcaatcta atatcattat
     5341 tggaacacat aacggctact ctgtcacttt caaagaagaa gcagtccgcg atatgggacg
     5401 tacagcttcc ggggttagag gaatacgcct gcgtgaggaa gattatgtcg ttggggcagc
     5461 cttaatgaag gaagatcaag aagtattagt gatcacagaa aaaggttacg gaaaacggac
     5521 aaaagtatcg gaatatccag tgaaaggccg cggaggtaaa gggatcaaaa cggcgaacat
     5581 cactgaaaaa aatggtccac tagctggatt gaccactgtt tctggagatg aagacatcat
     5641 gctgattaca gataaaggtg tcattatccg tttcaacgta tcgactgttt ctcaaactgg
     5701 acgttcaaca ttaggcgtac gtttgatgaa gatggaagct gatacgaaag ttgtcacgat
     5761 ggcagcagtc gaaccagaag ctgcagaaca aacagttgaa gaacaaggat cagaagaata
     5821 gccatcaaga ctaattgtgt aagctagcaa gaatgaccga ttaaaaaatc tggttattcc
     5881 tgctggcttt tttgattagt tgaatttctc ttaaaatatt cgaaactgtt cattctgtat
     5941 tgcttttgac caaaatagag tgtataatca gagcttgtga gtagtctaat taattagata
     6001 actctccttg ctcttaggaa cctaagagcc gaaagtccac agggaggtga atatcaatgg
     6061 aaaacacgaa atatgaaatc atgtacatta ttcgtccaaa cattgatgaa gaagcaaaaa
     6121 ctgctttaat cgaacgtttc gatgcaatct tgaaagataa cggagcagaa gttatcgaat
     6181 caaaagattg ggaaaaacgc cgtttcgctt atgaaatgaa cggttaccgt gaaggtatct
     6241 atcacatcgt taaagtatct tctccatcat cagcaaatgc tgtcaatgaa tttgaccgtc
     6301 ttgctaaaat caacgacgat atcattcgtc acatgattgt taaagaagaa gcttaattgt
     6361 tgtttcatgt gaaacaatag aaaacgatga gaggagatga ctattttgat taataatgtt
     6421 gtattagttg gacgtctgac gaaagatcca gatttgcgtt acacagcaag tggtacggca
     6481 gtcgcaacat ttactttggc tgtgaatcgt aattttacaa accaaagcgg caatcgcgag
     6541 gctgatttta tcaactgtgt gatttggcgc aaatcagcag aaactttagc aaactatgct
     6601 cgtaaaggaa cacttttagg tgttactgga cgtatccaaa ctcgttctta tgataatcag
     6661 caaggacaac gtgtatacgt gacagaagta gtagctgaca acttccaatt gctggaaagt
     6721 cgttctgcat ctgaaaatcg ccaacaaagc ggcggattcc aaagttctgg tcaatcagct
     6781 ggcggatttg gcggaaacaa taactctaat caaacatctc aatcatccaa cggtatgcct
     6841 gatttcgacc gtgatacttc tgacccattc ggctcaagtt caacgatcga catttcagac
     6901 gatgatttac cattctaaac gaatttgata ggagggaaat agaatggcac aacaaagaag
     6961 aggcggacgt aaacgtcgta aagtagacta cattgctgct aaccatattg aatatatcga
     7021 ttataaagat atcgaattac taaaaagatt tatttcagaa cgcggtaaaa tcttgccacg
     7081 tcgtgtaact ggtacaggcg ctaaaaacca acgtaaaatt actgttgcaa tcaaacgcgc
     7141 tcgtatcatg ggcttgctac ctttcgttag cgaagactaa taaatcctag gaattgaatg
     7201 actgatcctt ttgatcagct attcaattcc tttttttctg taaaaataac ctctattcgt
     7261 ttagtcgctc gaatgacgaa agttatggta aaatcaaatt agtcatatat gggaggaaaa
     7321 ttatggaaaa taaaaacaat catttatccc cgaaacctct cttccttatc ctgtttatat
     7381 taatcgaagt attggtagtc ttaattttac catttcgttt tgttagcgct tcggtcattc
     7441 tattgatgaa tttatggggt gttcgctatt ggttgaaaat gaaacgttgg cttattttaa
     7501 ataaacgtga aaaaatccag caggcttcga ctgtagctga aaccaatcta gcttatgtct
     7561 cagaagtcat gcctgtagga gtgattgctt atcagccatc aagccaaaaa atcgaatggc
     7621 tcaatccatt agcattgaaa atcaaagatc aaagtggatt aacagaggaa gaattgctag
     7681 atactttttt tactgcaaaa gaaaaaaaga aaaaccaagt ctatctagca gataaaacat
     7741 ttcagtttga tatggataca gtaaagggtg ttgtttattt taatgatatt acccaaaacc
     7801 agttgttaaa actccatcag acgaacagtc agccggtgat tggtatttta tctattgata
     7861 actatgcaga tgccattgat aaattggatg acaaagagat ttcttatttg aactctttcg
     7921 tcactacact agtttctgac tggatgaatg aataccatgt ctttttcaaa agaatcaacg
     7981 cagaacggtt tttcttcgta gcacagatga tcgatctgaa aaagatgatg gataaaaaat
     8041 ttgatttgct ggatcgcttt cgagcagaag ctgaaaaaca gagctcgcct atcaccctta
     8101 gtatggggat tgcttatggc tcaagaaccc tcgatgaaat cggtggagaa gctcagaata
     8161 atttagatgt tgctttagtc cgtggaggcg accaagttgt cgtgaaagag gtaaaagaag
     8221 gagcaaaacc tgtttattat ggggggaaat ctgctaagaa tgtcaaacgc acgcgtgttc
     8281 gttctcgagc gatgagtacc gctttgcgtc ggatttttga tgaaacaggc gatgtgttta
     8341 tcatggggca caagtttccg gatatggatg ctttaggctc tgcttttggt gtcagttgtc
     8401 tggcacgctt caaccagaaa aaagcttaca tcgttatcaa cgagaacgag atcattccag
     8461 atgtagaacg gtgtcttgaa gaaatccata agcatcctga attagaaatg caactaatct
     8521 ctccagaacg tgccatagag ctgaaaaaag aagaggacct cctcgtgatg gtggattacc
     8581 ataaaccttc tctatcgatc tctcagggcc tatatgagca atttgataag attgtgatca
     8641 ttgatcacca tcgtcgtggg gaagagtttc caagtaaacc tctgctgact tatatcgaat
     8701 cttcagcttc ttcagcttca gagctagttt ctgagctgat ccaataccaa agcagcaaaa
     8761 caaatcgctt agatcgtttt gaggccacat tattgcttgc agggatggtt gtagatacaa
     8821 atagtttcaa tgtccgtaca actgcacgaa cttttgacgt tgccagctat ttaaggtctt
     8881 gtggagcaga cgcatcctta gtacaatatt tattgagctc ggatttagcg acatacttgg
     8941 agatcagcaa tttgattgct ggaagcgact atgtgacttc tgatattgtt gtggcggcag
     9001 ggacagacga aaaagagtat aatagtgtta tagcagcaaa aacagcagat acattgcttt
     9061 cgatgatcaa tatcaatgca gctttcgtca ttacaaagcg gacagacggc ttgattggga
     9121 tcagtgccag aagtaccggc acgatcaacg tccaaatcat catggaatcc ttaggtggcg
     9181 gaggacactt cacaaacgca gctactcaac tggaaaatac gacagttgag gaaacaaaac
     9241 agaagctatt tgctgcaatc aaagaaaata tgaatcaaat atatgataag gagtaatcca
     9301 aatgaaagta atttttttac aagatgtaaa aggaaaaggt aaaaaagggg acatcaaaga
     9361 agttccagca ggttatgcac aaaattatct aatcaaaaat aacttggcaa aagaagcaac
     9421 gaaaagcagt atcagtcagt tagcaggaca aaagaaagca caagaaaaac acgaagctga
     9481 aatcttggaa gaagctaaaa agttaaaaga attcttggaa aaagaagata ctgtagtcga
     9541 attgaaggcg aaagcagggg aagacagccg tttgtttggt tcgatccctt caaaacaaat
     9601 tgctgaaggg ttaaacaaac aatacaaaat caaattagat aaacgaaaaa tcgaattaga
     9661 aaatccgatc cgttcactag gctacacaaa cgtacctgtt aagcttcatc atgaggtgac
     9721 ggctacgatc aaagttcatg tcgtacaaga ataacaaatc ttgatagcga tgttcagctg
     9781 gtaagtataa ccagaaggaa aaaagacaaa ccgaaattta tctgagaaaa cagctgcagc
     9841 aatgaaagca aaagctgcaa atcgatttca gattgatttc ggtttctttt ttgggaacag
     9901 caaaatagca taaagaaaac gagtataata aaagagaact atttttttat tacactttcg
     9961 gtacgaatga tgacttttct ctcattttct tgtaaaatga agcaattgaa gacaaggagc
    10021 gatacgtatg aatgaagtgt ggcaggatcg aatcccaccg caaaatattg aagctgaaca
    10081 agcggtttta ggctctgttt ttcttgacgc agaaacgatc attgatgcca tggagtacat
    10141 agagccaaaa gacttttatc gtcgtggcca tcaaattatt tttgagacaa tgatcgagtt
    10201 aaatgatcgt aatgaagcga ttgacgtggt tactgtcaaa gaccggctgg aacaagccaa
    10261 tttgttagaa gatgccggtg gtttaagtta tctctcagac ttggcattag cagttccgac
    10321 agcagcaaat atcgtctact atgcgaaaat cgttgaagaa aaatcattgc tgcgtacatt
    10381 gatccagaca gcaacaggaa tcgttacgaa aggattcgag caaggagaag aagttcaaac
    10441 aattttagat gatgccgaac gcagtatctt ggaggtttcg gaaaaaagaa atcgaagcgg
    10501 ctttttatct attgctgaag tactgaactc cacgattgaa aatatcgatc gactagctca
    10561 aaacaatgaa gaaatcacag gtctaccaac tggctatcaa ggtctggata aaatgacagc
    10621 aggtttccag cccgaagaac taatcattct tgctgcacgt ccagcggtag gtaaaacagc
    10681 gtttgcattg aatattgctc aaaatgtagg aacaaaaaca gatcgttctg ttgcgatatt
    10741 tagtttggaa atgggggcag aatctcttgt aaatcggatg ctttgtgcag aaggttcgat
    10801 tgaggccagc catttacgta caggtcagct ttcggaagaa gaatggtcga atctgatcgt
    10861 tgcgatgggg agtctttctc gagcaaatat ttttatcgat gatacaccag ggatcaaaat
    10921 ctctgaaatc cgtgcgaaat gtcggaaact agcgcaagaa aaaggcaatc taggcttgat
    10981 attgatcgat tatttgcagt tgattgaagg taacggtaaa gagaaccgtc aacaagaagt
    11041 atcagatatc tctcgccaac tgaaaaaact ggcgaaagag ctgaaagtac ctgtcattgc
    11101 tctttcacag ttatctcgtg gagtagaaca gcgacaagac aaacgaccag tgctaagtga
    11161 tattcgtgaa tccggatcaa ttgaacaaga tgcggacatc gtagccttct tgtatcgaga
    11221 tgattattat gaaagagaag gcggagatga cgatgatgga aacgagccaa aaaatgacaa
    11281 cgtcatcgaa gtcatcatcg agaagaaccg aagcggtgca cgaggaacag ttgaactact
    11341 gtttatcaaa gaatacaata agttttcgtc gctttcgcca agagaagaat tttaaagaag
    11401 aggttgtgag ccttgagcaa tcccgagagc tctcgtaaga agatgcaagg agtaaccgca
    11461 ggaacgacta gagtacttaa tttaaacaga tctccaaagg gattgtgaca aaagtcttgt
    11521 cacagtccct ttttccgaat aaacggtgtt caatcagctg acccttggta ttagctcagc
    11581 aaacgtcaaa atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg
    11641 ctgatcttac gacctctttt ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt
    11701 cacaatatca tcaaacatga gaagcagttt tttgaaattg ctccttgtga ctcagagtta
    11761 aacggcaggc tcacaacctc tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata
    11821 cgcccaatca aacgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat
    11881 gcaggacagc tttttcacaa cctctgattc acggtgttcc aaaagtagct tcttcgaaaa
    11941 ataagtcgaa atttcacaaa aatttgagaa gcaattttcg tacattcctt cttatttctt
    12001 gaagctgacc acttctgtta caacctcttt tttaataacc attctaaaaa gataaagttc
    12061 gttttttaag cgtttttata aaataaaaat aaaaatcatt cgattttatt tgaaatatgt
    12121 atctattatt gatacaatgg ttcaggatta aataatatag aacgaggtgt tcacatgtca
    12181 tctgtagtag tagtaggtac gcaatggggc gatgaaggga aaggaaaaat cacagacttt
    12241 ctcagtgaaa atgctgaagt gattgcacgg taccaaggtg gcgacaatgc agggcacaca
    12301 attaagttta acggtgtgac ttataaactg catttgattc cttctgggat tttttataaa
    12361 gacaagatca gtgtgatcgg aaacggcgtt gtagtcaatc ctaaatcatt ggttaaagaa
    12421 ttagcttatt tacatgaaaa aggtgtagaa acaagtaatt tgcgtatttc tgaccgcgcg
    12481 cacgtaatct tgccttatca tatcaaattg gaccaattac aagaagatgc taagggcgaa
    12541 aataagattg gtacaacgat taaaggaatc ggacctgcct atatggataa agcagctcgt
    12601 gtaggtatcc gtattgcaga cttattagat aaagaaattt ttgaagaacg tcttcgtttg
    12661 aatctagatg acaaaaaccg tcaatttgta aaaatgtttg attcagaacc acttgatttt
    12721 gacgatattt ttgaagaata ttatgaatac ggacaacaaa tcaaacaata cgtcacagat
    12781 acttccgtta tcttgaatga tgctttggat gcaggcaaac gcgtgttgtt tgaaggcgca
    12841 caaggtgtaa tgctggatat cgaccaggga acttatcctt ttgttacttc ttctaaccca
    12901 gtagccggtg gggtaacaat cggaagcggt gtagggcctt caaaaatcaa taaagttgtc
    12961 ggcgtctgca aagcctatac gtcacgtgtc ggtgatggac catttccaac tgaattattt
    13021 gatgaaacag gagaacaaat ccgcgaagta ggacgagaat acggtacgac aactggtcgt
    13081 ccgcgtcgtg taggctggtt tgattctgta gttatgcgcc attctaaacg agtttcaggt
    13141 attaccaacc tatctttaaa ctcgatcgat gtattaagtg gattggaaac agtaaaaatt
    13201 tgtacggctt atgaattaga tggcgaacta atctaccact atccagcaag cttgaaagag
    13261 ttgaaccgct gcaaaccaat ctatgaagaa ttgccaggct ggtcagaaga cattacagga
    13321 tgcaaaacac ttgcagaact gccagaaaac gcacgtaact atgtacgtcg tatctcagag
    13381 cttgttggtg tacgtatctc aacattttct gtcggacctg atagaacaca aacaaacatc
    13441 ttagaaagcg tctgggcgca aatctaagga taaaaacgac gactgaatca ggacgttctt
    13501 accagagcga gtcgaagtgt ttatctatac gataataata acttgaagag gtagtgaaag
    13561 agacgtttag ccttattgcc gataggctgt ctcttgaaca ctgtttattc agaataaggg
    13621 actgtgacaa aaaccgtgtc acagtccctt tttccgaaca aacggtgttc aaaagcttgc
    13681 atctacggca ctaagcccaa tcagatgaaa aatatggagt gctattttta tctgattgtt
    13741 cttactgctt gatgcagagc agctttttca caacctctcc actaaacggt gttctcctgc
    13801 caactcctcg gtcacaagtc acaatattca acaaacatga gaagctgttt tttgaaattg
    13861 ttccttgtga ctcggagcta aacggcaggc tcacaacctc ttggtaaacg gtgttcaatc
    13921 agctgaccct cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttttccgttt
    13981 ttttctctaa tactcaggtc aaaacgctga tttcacaacc tctgattcac ggtgttccag
    14041 aagtagcttc tacggaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa
    14101 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcttattt tgattagaac
    14161 ttgcgaaaat aaaccggtta aagtgtgtct agcgcaagag atttgctata atagacgata
    14221 agggtaaaat gtaacgagaa ggggttgaaa aatgtgtatc aaattgtaac agattcttgt
    14281 tgtgatctgc cataccatgt attagcaaaa gaaaaagttg attttttatc aatgaatctc
    14341 caattgaatg atcaagagtt agtagatgac ctcggaaaga cattcaatta tgaccgctac
    14401 atgaatcagc tgaaaaatgg tgcgatgcct acgacatcac aaatcaatgt tggccgatat
    14461 ttagaatttt tccgttctta tgcggaaaaa cagattcctg ttttatatgt ggctttttcc
    14521 agtggattaa gcggctctta cagtagtgcg cttcaagcgg ttgaattgct gaaggaagaa
    14581 tatgaagacc ctgagatcta tgtttttgat acaaaagctg caagtattgg ggaagggctt
    14641 ttagtattgg aagctgtaag attgaaaaaa gaaggtaaaa gcatcgaaga aaccctagag
    14701 tggctggaaa gtaattattt gaaagtccat tcatgggtaa cagtagacga tctgaaatat
    14761 ctagaacgag gcggtaggat ttccaaagca agtgctactc ttggcagtct attgagtgta
    14821 aagcctatta ttacggtgga taaagaagga aggctgacga atgttgacaa agttcgagga
    14881 agaaacaagt cgatccagac aatcgtcaat gagacggtaa aaacaatcgt tgcaccactt
    14941 gatcaaatcg tttatgtggc ttacgctggg gatctagaga gcgcggagaa ggcaaaacgg
    15001 cttttggaag agcagataaa gatgaaagat gtgaagcttt atcccttagg accaacgatc
    15061 gccagtcaca caggctatgg ctgtatcgcg attttttcga tgggaatcag tcgataaaag
    15121 cagtaactca ttctgtaaaa aagcccagaa attgattgta acaaaaacag cgactcttaa
    15181 aagatagatg agcatcctat ctcttagagg tcgctgtttt tttatttttt aagctaaata
    15241 ggccattatg cccataactc gttcaattgt ttttgaatat ctgcattttc caaaaattca
    15301 tcatatgtcg tatcagctcg atcgatcaca cctttatccg aaacggcaat gatgcggttt
    15361 gccaatgttt ggataaattg atggtcatgt gacgcaaata agattgagcc agtaaatgcc
    15421 atcaagccgt cattcaatgc tgtgattgat tctaagtcta aatggttcgt tggatcatct
    15481 aatacaagta cgttagcttt cgaaagcatc atttttgaaa gcatgcaacg gactttctct
    15541 ccaccagata aaacattcac tggtttcaat acttcttcac cagaaaacaa cattcttcct
    15601 aagaagcttc ttaagaaggt gttatcatct tcttctttac ttgcatattg acgcaaccag
    15661 tctaaaatcg ttagatcagt atcgaattct ttcgttgtat cctttggtaa ataagaatta
    15721 cttgttgtca caccccaacg tactgttcca gtatctggcg taatttcacc catgataact
    15781 ttgaatagcg ttgttgttgt tatatcattt tctgcgacga aagcgacctt gtcttctttg
    15841 ctcaaattaa aagatatatc atctaaaatt ttctttccat caatcgttac acttacattt
    15901 tctacctgca ataagtcgtt tccaatctca cgttcaggtt tgaatccaac gaatggataa
    15961 cgtctagaag aaggttggat atcgtctaaa gtaatttttt caagcatttt tttacgtgaa
    16021 gttgcttgtt ttgattttga tgcattcgca ctaaagcggg caataaattc ttggagttct
    16081 ttgatttgtt cttctttttt ggcatttgcg tttgcttgaa gtttagccgc tagttgacta
    16141 gattccaacc aaaaatcata atttcctaca taaagtttga ttttaccaaa atctagatcc
    16201 gccatatgtg tacatacttt gttcaaaaag tgacggtcat gggatacaac gatcacagta
    16261 ttttcaaaat tgatcaaaaa ttcttccagc caattgatcg attggatatc cagaccgttt
    16321 gttggttcgt ctagcaaaag aacatcaggt ttaccaaata gtgtttgtgc taataacact
    16381 tttacctttt gtccaccagt taattcactc atttgctgat ggtgcaattc ttcagggatg
    16441 ttcagacctt gcagtaaaac agctgcttct ggttcagctt cccatccatt aagctcagca
    16501 aattctcctt ctaattcagc tgctcgaata ccatcttcgt cagtgaaatc ctctttcatg
    16561 taaatcgcat ctttttcttt cagcacttca tacagacgct tgtgtcccat gatgactgtt
    16621 tcaagtacag tatagtcttc atagtcgtaa tggttctgtt tcaatacggc catccgttca
    16681 tctggtccca tagtaacgga acctgtggaa ggctgcagat cccctgataa cactttaagg
    16741 aacgttgatt tacctgcacc atttgcacca atcaagccat agcagtttcc gggggtaaat
    16801 ttaatattca catcatcaaa tagtttgcgg tctgaaaagt gcaaactcac atcatttaca
    16861 gtaatcaatt tttttcctca cttttatcat tattagtttt gacaagtctt atagaattat
    16921 ataggggaaa ggtaaaagat tcaaggcaat cgaacattct ttgtgtttca cgtgaaacag
    16981 aatgaaaaaa ataaaaataa aagcggagcg aaaatagcta ctataggatt tattagtttg
    17041 caacagcata atttttgacg ttatttataa gagggagtac tataaaactg taaaagaaat
    17101 gaaagaagga ttgaaaatgg atgaaaaaag aaccatcgac tggggctcat tgattttggg
    17161 aattttattt gtacttgtct cattgttatc atttcaagac cctgtcggaa atttagttgc
    17221 aattgtagta gtctttgcga tttttgcctt tattaaaggg atatttgaac tgtttgtacg
    17281 gaacagatta aaagagctaa caggatataa aggaaagatg cccttaataa tcgggatcat
    17341 cgatattcta gttggcgtct ttttcttatt caatattggt gcaggagtcg cagcattgcc
    17401 ttttgttttt gcagtatggt ttattgctga ttcagtttta gcgttattta ctgctgactt
    17461 ggcaagagga gtaagtgaag gatattattg gtttacgatc atcgtcaata ttttaggaat
    17521 tttattagga attttcttat tgtttaatcc aatttcttct gcgttgacat taagcttttt
    17581 agttggattc tattttatgt tatttggtat cacacatatc gtctatgcat ttagataagt
    17641 aaaatcaaac aaataaaaaa gccggcttcc gattacagtt ttactgttgt aattggaagc
    17701 cggctttttt atcatggaca aatatgcacg aggtgatttt taggtgtcga gacaaattta
    17761 tgcaaaaatg tgagaagcta tatacacaaa ttttatctta atactcgaag catagcgcag
    17821 ttgtcacaac ctcttttcat ataattcatt caatactggc aaatcatgga cttcatattt
    17881 ttctttgaat gcttggattt cttcttcaga atattttctt ggatctaaat gcagatcttc
    17941 tactggcaat gggcgcttgt tattgatctt cacatcgatt actacaggac gatctgattt
    18001 tgcagctgca tcaaaggcaa atcgaagttg cgagtattct gtgactgtaa agctatttgc
    18061 cccgagcgct tctccaactt taccgaaatc tgcatcttct aaaaatacac caaatttctt
    18121 ttgttccgta tcttcttgtt ctgcttcgat gaaaccaaaa gaatcattcg aaaagacaac
    18181 attaataatt ggaagctgat atttgacttg tgtaatgata tcttgcatga ccatagcaaa
    18241 cccgccatca ccacttaagg taaatacttg tttttctggg tagcttagtt gtgcggcaat
    18301 tcctcctggc acaccattac ccatagttgc gaaccatcca gatgtcgtat gtttttgttt
    18361 accgttcata ttcaataaac gaatagaatg gatagtcgtg ttaccgacat ctgtgacgaa
    18421 aatcgcatcg tcttcagcaa tacgattgat ttctttatag acagcctctg gacgaatcgg
    18481 ttcttcctct cgatcttcaa aagatttcaa ccaatttacc catttttctt tattttcttg
    18541 atttgctagg taccagctat cagcagggcg agcatcccct aactcgacaa gtctttgcaa
    18601 agctgtgttt gcatcaccga ggatcgacaa agatgtttca tggcgtcgtc caaattttga
    18661 cgcatcgata tcgatttgga taaattcagc agttggatta aaaaatgcac gtccaaatgg
    18721 gaagtcgcta ccgacaaata agatcagatc ggcttctgcc aatgcttcat ttccgggttt
    18781 agtagcgaca cgtccagcaa aaccaagaaa gttttcataa tcatcaggaa taattccttt
    18841 tgctagaacg gaagcgacaa caggaactga aaaatgttct gagaattctt tgatttgcgg
    18901 aaatccgttt cgcgttcctt gaccaacata aagaactggt ttttttgctt tttcaagata
    18961 aggcaaagca gctagcaaat ctttttcttc tggtaatatc acacttgttt tgtgagtgtg
    19021 agcggtggag aaaggttgtt cgtcaatttc ttcaaaacca agatcaacag ggatagtcac
    19081 gatagccacg cctttatgct cataggcggc tttaatggct tcatctacta catgcggaag
    19141 actttgtggt gtcatgactg tacgattgta tacgctaaca tctgcgaaaa taggattttc
    19201 atttagttcc tggaaagagt tatagttcat ggatgtagaa gcaacttgtc ctaagaacgc
    19261 tactagaggc acatggtcca tctgtgcatc atacaaacca ttgatcaaat gtgtcgcacc
    19321 aggtcctgca gagccgaaga cagcgccgac tttccccgtt aattttgcat ctgcagcggc
    19381 tgcgagtgcg ccgacttctt catgacgaac ttgaatgtag ttgatttcgg ttttttcttg
    19441 atatagcgca tccataattg aattgaaaga tcctccggga atgccgtaaa tatggtcgat
    19501 accccagctt tcaagaactt ttaccattgc gattcctgca ttgatttttg acatggtgtt
    19561 tcactccttt agcaatgatg atacataaat tttttgtatc tagagtatag aactttttat
    19621 cgaaaatccc aactaattcg tcttttcaca aaaatgtagt catgtttttg ggaatatgct
    19681 agagtagtac atagatagat tcaagagagg ggaatggcat ggttgaatgg ctattgagac
    19741 ttgaaccttg gcagcaagca cttgctggga cagcttttac ttattttatg acagctcttg
    19801 gtgccgggtt ggtttttttc tttaaagaaa tcaaaaaaga agtgctaaac ttgatgttag
    19861 gatttgcttc tggtgtcatg attgctgcaa gtttttggtc tttgttagat ccggctatta
    19921 caaaagcaga agaaaatgga gatattgctt ggctagttgt aagtattggg tttggtttag
    19981 gcggtgtgtt tttatatatg gcagataaaa cactgcccca tatgcatttt ggaccacaac
    20041 atgaagcaga aggattgcct acccatttaa aacggacgat cttgcttgtt ttttctatta
    20101 cattgcataa cattcctgaa ggtttagccg tgggggtagc attcggtgca gcagcaactg
    20161 cggataatcc gacagccgct atattagccg ctgtttcggt ggcgctaggt attggtatcc
    20221 aaaacttccc agaaggagca gctgtatcaa tcccgttgcg acaagaaggt ctgagtcgta
    20281 aaaaggcctt tgtttacgga caagcttctg gaatcgttga accaattgct ggagtgattg
    20341 gagcgctact agtaacgaaa gtggagctgc tgcttcctta tgcacttgct tttgccgcag
    20401 gagcaatgat ttatgtcgtg gtggaagaat tgatccctga agcacagcag acattgtcca
    20461 gcaaacatca ttttgcagtt tttggcgtca tgagtggatt tatcattatg atgattttgg
    20521 atgtggcatt aggataaata aaaaaaatat atatgatata tcaaataggg ccgcgaactt
    20581 atatcgcgac cctatttttt tagtaagtta tatcataatg aagtgaatgg tatcatacat
    20641 taataatagt tttttctatg tgtatttcct ttgatccagc gaacaagcca gatgataagt
    20701 gcgattggga ttagaacttt gaccgcaaac cataaaagcc cgcttaggac actgacaaaa
    20761 agactgatga cgaagctagc tgcaattagc actaaaagaa ccatgaagat atttaagaac
    20821 atattttttt gacgcataca aaaccctcct cttgttcatt ctttactata gaaaaagtat
    20881 aacagggatt gtttaaaaac acctccaact gaagactgat ttttacttca tctacttaaa
    20941 tgatcaaaca attgcttttt ctctggttta tcatctaaaa tagagcggaa aaggaggaga
    21001 atatcatgcc taaaaatcgt cttgaagcat ttacagatgc tgtaatcgcg attatcatga
    21061 ctattttagt tttagaactt catgaaccta acggagacac gtgggaggcg ctcgctgcat
    21121 taggttaccg attttttatc tatattgtca gttttatcat tttagcgatt tattggaaca
    21181 atcatcatca tttatttcaa gttgtacata agataaatgg acgggtctta tgggcgaaca
    21241 gtttctttgt ttttgcgctt tcgctttttc cgtttgcaac ttcatggatc agtgagtaca
    21301 tcaatagcct ggctccagaa atgacttatg gttttgttac cttgaccgct aatattgcgt
    21361 attatatctt ggcaaaagaa cttgtcaaag caaataaagg ccacgagaaa gcattgaagg
    21421 cgatgttgaa taattacaag aaatcgtatc tatcgattgg tttgaatgtg ttaggaattg
    21481 tcttagggta tttagtagcg ccagtagccg tgctgatcgc aaacatcgtg attctagctg
    21541 cttggatcat cccaagtcgt accatcgaat cacaatatcg gtaaaatgat ccgtgtttaa
    21601 aatttaagag aaaaatatag aaaaaataaa taaacgatca aatatccgag tgatactagt
    21661 attcaaagag ggatgcagat ggatcaaaaa agaattcatg taggactgat tactagtttt
    21721 gtcacgatgc ttatttattt ggatcaaggt catttgactt ggacagtagt tggtgtcgta
    21781 ggatttttga cattattcac agcaataaaa aaataaacat ctttatgtaa tctgatgttc
    21841 tatctattta agaaaagcat gtttacagca gtatgattct gttgtaaacg tgtttttatt
    21901 tgtggaaaat ttaattttta gaaaagtgta tcgaaaaata gatagttagt ttttccacag
    21961 tggataactt cgtaagagat aaaaataaaa aattagtcaa agaagg
//
LOCUS       DAJSKS010000036        21439 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJSKS010000036 DAJSKS010000000
VERSION     DAJSKS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.037"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(165..2546)
                     /locus_tag="ON396_001162"
     CDS             complement(165..2546)
                     /locus_tag="ON396_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCR3113262.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2536..3183)
                     /gene="recU"
                     /locus_tag="ON396_001163"
     CDS             complement(2536..3183)
                     /gene="recU"
                     /locus_tag="ON396_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCR3113263.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3247..3786
                     /locus_tag="ON396_001164"
     CDS             3247..3786
                     /locus_tag="ON396_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCR3113264.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3876..4295
                     /gene="gpsB"
                     /locus_tag="ON396_001165"
     CDS             3876..4295
                     /gene="gpsB"
                     /locus_tag="ON396_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCR3113265.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4317..4684
                     /gene="rnpB"
                     /locus_tag="ON396_001166"
     ncRNA           4317..4684
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ON396_001166"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4893..6059
                     /locus_tag="ON396_001167"
     CDS             4893..6059
                     /locus_tag="ON396_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCR3113266.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6105..7601
                     /locus_tag="ON396_001168"
     CDS             6105..7601
                     /locus_tag="ON396_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HCR3113267.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7682..8365
                     /locus_tag="ON396_001169"
     CDS             7682..8365
                     /locus_tag="ON396_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3113268.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9010..10200
                     /locus_tag="ON396_001170"
     CDS             9010..10200
                     /locus_tag="ON396_001170"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR3113269.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10302..11255
                     /locus_tag="ON396_001171"
     CDS             10302..11255
                     /locus_tag="ON396_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR3113270.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSGNVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11324..12673
                     /locus_tag="ON396_001172"
     CDS             11324..12673
                     /locus_tag="ON396_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3113271.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12856..13179)
                     /locus_tag="ON396_001173"
     CDS             complement(12856..13179)
                     /locus_tag="ON396_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113272.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13308..14471
                     /locus_tag="ON396_001174"
     CDS             13308..14471
                     /locus_tag="ON396_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR3113273.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14541..15890
                     /locus_tag="ON396_001175"
     CDS             14541..15890
                     /locus_tag="ON396_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR3113274.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15935..16366)
                     /locus_tag="ON396_001176"
     CDS             complement(15935..16366)
                     /locus_tag="ON396_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3113275.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16754..19396
                     /gene="alaS"
                     /locus_tag="ON396_001177"
     CDS             16754..19396
                     /gene="alaS"
                     /locus_tag="ON396_001177"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR3113276.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKEHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19596..20192
                     /locus_tag="ON396_001178"
     CDS             19596..20192
                     /locus_tag="ON396_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3113277.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20314..>21439
                     /locus_tag="ON396_001179"
     CDS             20314..>21439
                     /locus_tag="ON396_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR3113278.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITF"
BASE COUNT         6919 a         3788 c         4536 g         6196 t
ORIGIN      
        1 atggattttc taatttttaa gctaaatgtt agatcacaca tgtatatgct ggatagtctt
       61 ataaaaaatc atgtaataca gcttggtaca tgatttttat ttgtagctgt tttttacata
      121 gacacaaaaa agaaaccacg cttgtagacg tggtttcttt tttactatct gtttccagaa
      181 gctgaggtgc tgctggatgg tgtatggtca tttgcagcat ccgctccgct agttgctggc
      241 tcactggaag aagcaggagg agcagatgaa gacggtgtcg agctttcttc ggatgaagaa
      301 gaaggaaccg ttgttgaaga cgctggctcg cttgtttctg ctgtcgtgct ttcttttgag
      361 gactcagcag ttgattcgct ttgactagta gagctagagc tttgagtcgt tgtctctgtt
      421 gttgagcttc caggagtctg gacatagctg gaagatggaa tagttgttga tggtgtaatt
      481 gcattcgatc tagctgtata ttgatctttg tagtacagtt caccgcccac acgaattaat
      541 ccgcttggca tctcccagtc tgtatttgtc acgttagctg aaacatactg cataagctcg
      601 cgataaacat cagaggctac gtgtgacgat tctgaagtga caggtgtcat ttttttattg
      661 taacctgtcc acacggaaat cgaataattt ggcgtatagc ccgcaaatag gatatctgga
      721 tacaccccag aagaaatacc aagttttgca tactcgtcat ctgtgtaatt ggatgttccg
      781 gtcttaccgg cttgatataa cccagcgatt tgcgcattcg taccagttcc ttcagtgatc
      841 gtatctttta aaatgtctgt gatcatataa gctgtttctg gagacatggc tgtttttcca
      901 tctggctcat aagtttcttc tgttccgtct tggaagacga ttttattaac atattgaggt
      961 ttgtaataag tccctccatt ggcgaaagcc gcataggctg ctgccatttt taaggaagaa
     1021 gctccgtatt ttgtcccatc ttgttcttcc gtattactag agatcgcatt cgattgatgg
     1081 atcgtactgt attcgatccc aagatttttt aagaaagaag ccactttatc tgaaccgact
     1141 tcattgaata atttgactgc tggaacatta cgggataagt aaagtgcttg gcgtaaagta
     1201 atggtgccca tatattgatt atcccagttt cctacaggag tcgaagtccc ttcatagtta
     1261 tacggtgcgt cagtaatagt ttttccagta gaatatttca aatattcaaa agctggtccg
     1321 taatctgtga caggtttcat cgtagaacca aaatcacggg aagtattcac tgctaagttg
     1381 ttgcctaaag ttacgtcttc tgcgatgtga cgtccgccaa tctgggcttt tactttcccg
     1441 gtattggtgt cgataagggt ggaggcaact tgcatctcgt cgtctgggta agagacatac
     1501 tgatccgtgt tgacgatatc atatagtttc ttttgggcgt ccagatccaa attcgtataa
     1561 atctctaaac cgtctgtgta gacattctta tctgtttttt cttgtacttc attgatgact
     1621 tctttgacat aattatcgac gatcttagta ttgtcatcgg attgagttag ttcttgcaga
     1681 ccatccgtta ccggtacatt aacagcttga tcatattctg tttgagagat tttttcgttt
     1741 tgaagcatcg tatacaaaac agtatctcgt cttttcttcg cctgatctgg ataaacataa
     1801 ggatcatagg cggaaggggc ttgaggcatc ccagcaagca atgctgtttg cggtaaagaa
     1861 agttctgata atttcttacc aaagtacatc tcagaagctg tctccatccc gtacaagccg
     1921 tttgacatat acactttatt tacgtagtag gtcaagatct cttgtttcga ttttttttgt
     1981 tccaatctta cagccatcca cgcttcttgg gccttgcgtt tcagagtctg atcctcggca
     2041 ctggtggaga agaaggaaag tttgatcaat tgctgtgtca aagtacttcc gccttgcagt
     2101 ccgccagagg taaagttaga gagggcagaa ccgatgatcc gaatagggtc aaccccgatg
     2161 tgtttataaa aacggcgatc ttctaccgac acgatcgcat cttctaatgt tttcggtaat
     2221 tcattcgcag aaattttttc acgcttttct gcgcctaaat cttcgaatag ttcgccatct
     2281 tgtgtatata gtttggaaga tacggtagcg tctaattttt tatccgtcaa ttcgggcgca
     2341 tcttttgcgt aataccagaa aagtcctaca cctgctaaaa agaggataca gaaaaatgag
     2401 agtattccta aaaggatctt gataaataat ccttttttat gtgttccaga ggactttcca
     2461 gaacctttgc cagaattctt cttcacactc tttttaggag taggctgttt ctgtcgtcta
     2521 gagcttctag tttgttcatt tgccattctt gctttctcct tcaatagata atgttgataa
     2581 atagaagtca accgcgtcca aataaggaat ccgcggagaa ataccagttt tgatctctat
     2641 tcctagttct tcgatcaaag ataaaggtag tgatttgcgt cctttttcct tttgggcgtc
     2701 ccagtaagca atcaaatgaa cactattcaa ataaaaacag cgcttcaaag acgaaaacca
     2761 taacaagaca aagcagaccc cattttgttc gaggcatgct ttcatatgtt cgatttgatg
     2821 ggcatggaag tttttaaagg gaaaagatgt tttgttcttg gtttcttttg cttcaaagtc
     2881 caagtaaaag cctcgataga cgccattata gtctgtagtt gaggcttggg taaagtaggc
     2941 ttccttgatc acagcagcac ttctcttagg atagtctact ttcacgattt ggataggggt
     3001 aggcttttta tggataaccg ccaacccgcg ggaaagataa tatttattac tttcattgat
     3061 tgcttcttca aagcgcatcc cccgattgcc gaattcagtg atctgatttc ttttttcttg
     3121 aattttttcg acgggagagg cattcttata ataaggttgg ccatttggat agcgcaatac
     3181 catctttaat cacactccta ggccacatta tagcaaaaaa gcggatagaa agaaaaggaa
     3241 tctgagatgg ataagtcaaa agtaatctat gtgtctgggt atcgaagttt cgaactcggt
     3301 gtttttaaag aaaatgatcc taaaatccaa gttgttaaaa atgttttaaa aaaagaactc
     3361 catcagttag cagaagaagg gctggaatgg gtacttgtca gtgggaattt gggcgtcgaa
     3421 ctatgggctg tgcaaatagt agcggaattg aaagaagaat atccggaact atctcttggt
     3481 attttgtatc ctttttctga ttttggtaat aactggaatg aagggaacca agaaaaaaaa
     3541 caattagcag aacaattagc agattatgtc gaagctgtta gccatcaacc ctaccaatca
     3601 cctagtcagc taaagaatca cactcgtttt atacttgagc atacagtggg ctgtctgctg
     3661 atctatgatg aagaattccc tggaaaaacg aagtattttt ttgaagatgc acagaattac
     3721 caagcagatc atccctacga aattcggctg atcagtatgg acgatcttca aaattttagt
     3781 gaataatcga tattgtgttt gatttatcaa cttttttcct atacaatgaa aaaggtagaa
     3841 atgttattac gtaaatgaaa actagaggtg taaatatggc gaatttggtt tatagtccta
     3901 aagacatttt acaacaagaa ttcaaaacaa aaatgcgcgg ctatgatccc gttgaagttg
     3961 atgaattctt agacaatatc atcaaagatt atgaaactta cagcaaagaa ctccttgctt
     4021 tacaagaaga aaacgatcga ttgagtgcaa aagtagctca attatctaaa actcaaggag
     4081 cagctcaaac acgcgtgcaa caaacagagg caccaaagag cgcagcagtg accaattttg
     4141 atatcttaaa acgcttgtca aatcttgaac gtgaagtatt tggtaaaaag cttgatcaac
     4201 aagcaagtgc agtcaaacca gcacaaccta atccaaataa ctacacaaat gcagacacaa
     4261 gcttagatga taatgaaaaa acacgtcaat tttaattttt gattaatgaa tttgcggttt
     4321 tcgggtaatc gcggtctgac ttgatcaggc tgaggaaagt ccatgctcgc acaggctgtg
     4381 atgcctgtag tgttcgtgct tagcgaaacc ataagctaag gtactctttg gagtaacggc
     4441 aggaaaacaa gctaaggctt cggctatgct taagtatcct tgaaagtgcc acagtgacga
     4501 agcggctggg gaaacccatc cggtggaacg cggtaaaccc ctcgagcgag caacccaaac
     4561 aatggtaggg gcgctttttg tctggaagaa gaacaggaca aaaaggcagt ataactgcag
     4621 atagatggtt accgctttgt tttttctccc tggaaaagca aagtacagaa catggcttat
     4681 agaaaaccgc aaacatcagg gaattaggtc gtgacaaaag taatgcgtta taagaaacaa
     4741 gaaaaacatt tgaaagctat ttttcaatac ttcagatgaa tcgttcttat tacaaaacaa
     4801 acttttgtat caccggttat tcggaatttg agtaaaagtg tggggtagga ctgatgtccc
     4861 acctcctttt tttataaata aggagattgt gtatggaaaa aaacaggaag ttcaacctcg
     4921 tcgcaactgc ggcaagcggg ctagaagcac tggttggtaa agaattaaga gatcttggaa
     4981 tcgaatgcca agttgagaac ggacgtgcac gttttgaagg aacaatggaa acgatcgcta
     5041 cagctaattt atggttacgt acagcagatc gagtaaaaat cgtggttggg gaattcgatg
     5101 cgtatacgtt tgatgaattg tttgaaaacg taaaagcatt gccttgggaa gattttcttc
     5161 cactagatgc agcttttcct gtagctggaa aatcgatcaa gtcgaaacta tatagcacac
     5221 ctgattgcca agcgatcaca aaaaaagcaa tcgttgaacg tctccgtacc tattatcacc
     5281 gtccagcttc tgtaccactg acggaaactg gcgcacattt tcagttggaa gtagctttgc
     5341 tgaaagacca tgtgatggta actctggata cgacaggacc aagtttattc aaacgtggtt
     5401 atcgcttaga aaaaggtgga gcaccattga aagaaaacat ggcagcagca cttattatgt
     5461 tgacaaattg gcgaaaagac cgtccatttt atgatcctgt atgtggttca ggtacgcttt
     5521 gtattgaagc agccttaata ggccataata tcgcaccagg attcaatcgt gagtttgctt
     5581 gcgaatcttg ggattggttc agtcaagaaa tcatggaaaa tgttcgatca gcagccgaag
     5641 aaaaagcaga ttatgatatt gaattagata ttactggttc agatatcaat ggacggatga
     5701 ttgaaattgc caaagccaat gcagaagaaa tcggattagg ctcttcgatt acctttaagc
     5761 aacaagcagt caaagacttc aaaacggata aagaatacgg ggtcatcgtg gctaatccgc
     5821 cttacggaga acgtctagga gaagaagaaa ctgttcggaa attatataaa gaaatgggcg
     5881 atgtattccg tccattaaaa acatggagta aatatatctt gactagcgac ttagcgtttg
     5941 aggaatttta cggacaaaaa gcaaccaaaa aaagaaaatt gtacaatggt gcattgcgta
     6001 cggatctatt ccagtattgg ggaactcgtc cgcctagaaa aaaagagaaa gacgaataat
     6061 cttactaatt gattcattca attggatgtt tggaggaata gggaatgaaa gaaaaagagt
     6121 ttttacaaga agtaaaagaa atcaatcttc tacaacaagc tttaggaatc ctcgattggg
     6181 atacacaaac aggtatgcct gaaaaagcta gtgcataccg aagtgaagta gacagctatc
     6241 tttacagcat ttatttttca aaaaaaatcg gtccaaaaat caaagaagcc attcgctatt
     6301 tttctgaaca tccggaagaa ttgtcggaag ttggaactgc cgtttttgcc aaagtaaaag
     6361 aagagtatga tttggaacat cgcgtaccgg aagatcttat gagaaaatat acagctgccg
     6421 tttcttcagc acacggacaa tggcaaaaag ctagagaaac acaacaattt tctgattttc
     6481 aacacgcatt gaatcaaaat attgaattaa caaaacagtt gataccttac tggagaaaag
     6541 atgaaaagac gaattatgat gttttgctga atcaatacga acccgggatg actgtcgaaa
     6601 tccttgataa ggtatttgat cagcttaaag aaggaattct ttcgatccgc cgtacgctcc
     6661 aagaaaaagg gactgaacct gaaacagact ttttaaatcg gagaatgacg aaaaaacaac
     6721 agaagagatt tgtagtgaaa gtaatcgaac aacttggtta tgattttagt cgtggtcgtt
     6781 tagatgacac tgtccaccca tttatgactg gaattaatcc gaatgatgca cgaatcacga
     6841 cacgatggaa cgaaacagat ttcaaaatgg ctgtttttgg agtgatccac gaggctggtc
     6901 atggtatgta tgaacaagac atcgatgata aatatgcgta tacaccggtt tatgaaggta
     6961 catcaatggg tatccatgaa tcacaatcgt tgtttaacga gatcattatt ggaagtagtc
     7021 gcagcttctg gaaaaagcaa tatcctttct ttcaagaatg tgcagaagga acattcgatg
     7081 atattccttt tgacaaattt tatcgttcat tgaaccatac aaaatcaagt ttggttcgta
     7141 tcgaggcaga cagtttgact taccctctcc atatcatcat tcgttatgaa atcgaaaaaa
     7201 tgattttcaa tgaaggggta gatgttgctg aactacctga aatatggaat caaaaatatg
     7261 aagagtatct gggaatacga ccaacaaatg atgtggaagg catcctacaa gacgttcatt
     7321 ggtcaggtgc aagctttggt tattttccct cttatgcatt agggtacatg tatgcggcac
     7381 aattacttca ttctttgcag caagaaatag atgtggaaaa tgttctagcg tcagacgatt
     7441 attctccaat caaaaactgg atgtcagagc atatccatca atatggtgca tctagaaaac
     7501 caaatcaatt aatcatggat gcaacacatg aaccgcttaa tccgcaatac ctgatcgact
     7561 atatgaaaga cctgtatttt aatgtatatc aggtaaaata aaatacaatt tacaaagata
     7621 gctgaaattc atctaatcta tagatgtttt cagctattta cttaagaaag aagtggaacg
     7681 aatggataaa ttacaagtga tcgctgatta tagtatggac caattagcgc atgatcaaac
     7741 aggacatggg agcgatcata ccaaaagagt tgtcaagtta gctgaaaaaa ttttggagac
     7801 ggaaccgcag gcagaccgct ttgtgacgct tgcagcagct tacctacatg atacgattga
     7861 tgataaagtc gttaaaaatg agaacgaggc aaagcaacag cttcgtgtat tccttcgctc
     7921 tttgccaata gtagaggaac agatctcgat gattttttcc attatcgaaa atatgtcctt
     7981 ttctaaaaat ctctctgaag cggccgaact atctctggaa ggtaagatcg ttcaagacgc
     8041 tgatcgtatc gaagcattag gagcaatcgg cattttgcgt actgcttatt tcggcggtgg
     8101 acatgggcac ccgatttttg atcctgaact gtatccgcaa acattcaagg acaaagaaaa
     8161 ttaccgaaaa ggcacgacag tgatcaatca tttttatgaa aaactgttct tgctgtctga
     8221 taaaatgaat actgattacg gatatgaaga agccaaacga agagaaacct tcatgcgtga
     8281 attcttagaa gaattttttc tggaatggtc tattgacgat gaaaatttta ctccagacaa
     8341 ttggatcaaa aataacaaaa aatgatggag gttgtgacag aagtggtcag ctccaagata
     8401 tttcggatca ttttccgtaa aagttacttc tggaacactg tttaccaaga ggtcgtgagc
     8461 ctgccgttca gctccgagtc acaaggaaca atttcaaaaa atagcttctc gtgtttgatg
     8521 aatattgtaa cttgtgattc ggaattggca ggagaacacc gtttattcgg aagaatagga
     8581 ctgtgacaag acttttgtca cagtcctttt ttcgaattca acaagtaaaa cttttaagaa
     8641 atattcagag agtaaaatta aaaattgctg ataaacgaag atggaaattt tatgtatttt
     8701 tattggtcta tgcaataaat aaattaaata ttcagaatac aattgatttt aacgaaaggg
     8761 cttctatcaa tggttgtgtt gtatttatct gcccaggtag agtaaaatag atcattatct
     8821 aaaaggtaat tgttcatact atataagtca tttgaaagta aaaagatact gtaaataaaa
     8881 aatgtggtta aataattctc ataatcagtg taagcgttat caaaaatata gcatacttgc
     8941 tttgtagatg aatgacacga tatatgtttg gtatgttctg caagacagat gaatatggag
     9001 gtggagacaa tgtcttccaa agaactaaca atgtatttag agaaagaatt ggatcaactg
     9061 aaagaaaagg ggttatacaa tacgatcgac atactagaaa gtgaaaatgg tgcttgtatc
     9121 atcgtagacg gaaaaaagat gatcaatcta gcatccaaca attatcttgg atttgccaat
     9181 cgcgaagaat tgaaaaaagc atgtattgaa gcaaccgaaa cttatggtgt aggagcagga
     9241 gcagtccgaa cgatcaatgg ttcgctgaaa atccatcagc aattagaaga aaaaatcgca
     9301 gaattcaaag ggacagaagc agcaatcgcc tttcaatctg gattcaactg caatatgggc
     9361 gctatctctg cagtaatgac aaaagaagat gctatcttat cggatgaatt aaaccatgca
     9421 tctattattg acggttgccg cttatccgga gcaaaaatca tccgaattaa acatcaagat
     9481 atgaaagacc ttgagaaaaa agcaaaggaa gcgatagaaa gtaagaaata taaaaaaatc
     9541 atgtatatca cagatggtgt tttctccatg gatggagaca ttgcacgatt gccggaaatt
     9601 attccgattg ttgaaaaata tggactgatc acctacgtcg atgatgcgca cggctcaggc
     9661 gtaactggaa aaggtgcagg aaccgtcaag cattttggat taagcgacaa aatcgatatg
     9721 caaatgggca cgttatcgaa agctataggt gttgttgggg gttatgtagc tggatcgaaa
     9781 acactaatcg actggttgaa agctagaagt cggccattcc tattttctac ttcgctaact
     9841 ccaggtgcag ctgcagctgc attggcttcg attactttga tgcaagaaca tccggaactt
     9901 gtcgaaaaag tttgggaaaa tgctaattat tttaaagaag aattgaaaaa agtaggctat
     9961 aacatcggta tgtctgaaac acctattact ccagtcattt tgggagacga aaaagtgact
    10021 cagactttct ctaagaagct gatcgaacat gggatttatg ctaaaccaat cgtttatcca
    10081 actgtgcctc ttgggacagg caggatcaga aatatgccga ctgcggaaca tacgagggaa
    10141 atgcttgatg aagcagtagc agtttatcaa aaaataggaa aagaaatgga aattatttaa
    10201 ctaaatttag ttttttcgtc tatttaaaat tagagaacat gttcacgcat atattgttaa
    10261 tcatctgaag atagtagtaa aaaagatcag ggaggatcat catgaaaaaa gtattggtga
    10321 caggatgttt aggacaaatt ggttcagagt tgatcacacg gctacgtcaa gaactaggca
    10381 gtggaaatgt cattgcgaca gatatacgca tgcctgaagt cattgagaaa aacgatatct
    10441 ttgagatcct agatgtaatg gattacgaaa aaatgcgcag catagcagaa caatatcaag
    10501 tggacacatt gattcatttg gctgctcttt tgtcagctgt tgcagaagca aaaccacaat
    10561 tagcctggaa tttgaacgtg actgggctgg tcaatgcatt ggaagtcgct agagaattcc
    10621 agctgaaatt ttttacgcca agttcaatcg gcgcgtttgg tccaaatact ccaaaagatc
    10681 ataccccaca agatactctt cagcgtccta caaccatgta tggggtaacg aaggtagctg
    10741 gagaactact atgtgattat tattatgtga aatatggcgt agatactcgt ggtgtccgtt
    10801 tccctggatt gatttcttat gcaaccttgc caggaggcgg tacgacagac tacgcagtcg
    10861 atatttatta ttcagcaatc aaagaaggaa agtacactag tttcattgat caaggtacat
    10921 caatggatat gatgtatatg ccagatgcga tcgatgccat tataaaactc atgaatgcag
    10981 atcctgatcg attggttcat cgaaatgcct tcaatatatc ggcgatgtct tttgacccgg
    11041 aagagatcaa agcatctatt caaaaaatca taccagattt tgaaatggat tatgatgtgg
    11101 atcctgtacg tcagtcaata gcagaatctt ggccaaattc actagatgtt tcttgtgcgc
    11161 aagaggaatg gggcttttcg ccgaaatacg acttagatgt gatgacaaag gatatgttgg
    11221 aaaaactgaa agaaaaattg gtagagacgg tataaaacaa gaaccacatc caaaaagttt
    11281 tttatacttt tgggattggc tatggtatga aaggggacaa gggatggaag aatatcaagc
    11341 cacgccagta aaagaagtaa aaatagaaaa tgaattaaaa cgtacgatgg gattttttac
    11401 tgctttatcg actgttatgg gcacagtgat cggtgcaggg gtatttttta aagccgcaag
    11461 cgtagcagaa gttacaggat ccgccagttt gcatatgttt tcttggtttc tcggtggtat
    11521 gatttctgtc tgtgcaggac taacaggagc tgaactggca gctgctattc cagaaacagg
    11581 agggatgatt aaatatatcg aacggattta cggaaataca gcagcttttt tattgggctg
    11641 ggcgcaagtc gtgatttatt ttccagcaaa tgtagcagcc ttgtcgatca ttttcggtac
    11701 acagttcgtc aatctatttg gtctttccca gtcaatgatt gttcccgtag ctgttacagc
    11761 tgctgtatcg atcctgttga tcaatttttt aggttctaaa gctggagggg cttttcagtc
    11821 gattacgctt gtctgcaagc ttattccttt gtttgtgatc gtcattttcg gtttgttccg
    11881 acaagaagga gtagacttcc aactgtttcc gatccaagca ggagaaaatc tttcattttt
    11941 ctcagccttg ggagcgggct tgctagcaac gatgttcgca tacgacggtt ggatacacgt
    12001 aggaaatatt tcgggagaat tgaaaaagcc ggcgaaagat ttgccaaaag caatttcttt
    12061 aggaatcatc ggaatcatga tcgtttattt attagtaaat gcggtgttct taagaaccgc
    12121 atctatcgac ggagtcgcag gaaacagcaa tgcggcaagt gacgttgcaa aaatgatttt
    12181 tggcggcttc ggtggaagac tggtcacagt tggtatcttg atttctgttt atggaaccat
    12241 caatggctat acccttactg gaatgcgtct cccttatgtt atggcaaaag aaaacaacct
    12301 gccttttagc aagctctttg ctaaacttca tgataaaact aaagttcctg tagccgcagg
    12361 tatcttagag ctagtcatcg ctattggcat gatgatgatc ggtggttttg atacactaac
    12421 ggatatgctg atctttgtca tttggatttt ttatacgatg gtctttgttg gtgtcattct
    12481 tcttcgtaaa aaagaacctg atttgtttcg accatacaaa gttccaatgt atccgtttat
    12541 tccactagta gcaatcattg gagggacttt tatcgtatca agcacgctta tcactcagac
    12601 ctttttagct tcgatgggga tagtattgac acttgctggt atcccgattt atctctattt
    12661 aaaacgtaga tagctaaaac attcgaattt tattagaact gcacttctct gccaaacaaa
    12721 atattttatt taaatgagga gtacgtggaa gaaatgtcaa aagtactcgt taattttgat
    12781 cacgtaaatg tcgttttgtc tacgatgaaa tagcaaagta aaagacagaa atcgggctca
    12841 accgatttct gtcttttact ttgatttgcg taactgaact attcggcgta cgccgcgata
    12901 aatatttaat aaaaccaagc aaccagaaat gaaaatggga agccaaatca gatagacagg
    12961 gtagccaagc actttagaca aagcatgtgg agtcatttta tccatttcaa gaagtgcttg
    13021 ctgccaagtc tgaagattga agactagcat gaaacccgaa ataatcagga gaataaatcc
    13081 taaataatga attgtagttc tttttcttac ataagcgatc gtagcataaa ggaacagtaa
    13141 aaaaataggt acgccaaaaa caaagtacat attcagcatc tcgacacccc ctttgtgttt
    13201 atcttacttt actttgaaag aaatgagaaa taatagcctc gttacttgct tgaaacagca
    13261 aaggactgct aagataagag agatacaaag aatcaaggag gagtcatatg gcaagttata
    13321 tcgaaccgat tcgttttgcg ctgattgttt ttccattttt agccttagcg atctcatctg
    13381 ttttttttat ttatgaatat cgaaagtatg gaacatttct agtaacaagg gcagttattt
    13441 tgtattcttt cgttttttat ttattgtgtg cgtatttttt ggtgatctta ccacttccgg
    13501 caaaggagac ggttgcccaa atgacagggc caaagatgga gctgcattta tttgcttcat
    13561 ggcaacattt cagagacaac acggtattgg tcttgtctga acctagtacc tacctgccgg
    13621 caatgaagca gagtgtattt ttggaaccag cttttaatgt tttattactc ataccatttg
    13681 gcatttattt aagatattat ttccggcttt cttttatgaa aacgatgatt gctagtttct
    13741 gcctttctct attttttgaa ttgacgcagc taagtggact ttattttatc tacccaagac
    13801 cataccgctt atttgatgtg aatgatttag tgacaaatac attgggtgga atcattggtt
    13861 ttgtcgttac gccgttattt acatttatgc ttcctacaag aagacgaatg gatgaacttt
    13921 cttatgaaaa aggaaagact gtctcattga ctcgacgctt ggtcgcttat ttgatcgact
    13981 ggacattttt atctcttatt aatttggtag cggcgatttt ctttaaaatt gcgttcgatt
    14041 caggggcagt tacttctcat atctggtggc ttttattaga agtaggggta tactttatga
    14101 ttgtaccgtt tataaccaat ggatggacac ttggcaaaaa aattgtgcgt atccgtttgg
    14161 tggaagaaga ccgcgaacga atttctttaa aagcattgat gatccgctac gggtatctgt
    14221 actttctttt ttatggttta agctggttgg ggcagttgct tcagcaaatc atgaattcga
    14281 caacgaatac ggtgttgcag atgagtgcag tattcatttc tctcggtgtc ttgttactac
    14341 aaggtatttt tattttaaat atcttgctat catttttgag aaaaaaacgg attctgtttt
    14401 atgaaaaagc tagtcacaca tatactgtta gcacgattgg ccaaacaaag gatgatgatt
    14461 ctcgaaatta gctgagcaaa aacgctttgt ctgtgttata atgccaaagt cgtgataatt
    14521 taaagagaag aggtgcacga atgaataatt ttgatatatt ccgcttcaaa ccgtttatca
    14581 atgaagcgtt aaccgcaaag ggcttcaaac agcccacaga ggtccaggaa cgtttgattc
    14641 ctttaattgc aaaaggaaaa aatatcattg ggcaatctca aactggtaca ggaaaaacac
    14701 atacgttttt gttgcctttg tttgaaaaaa tcaatgcaga aaaagaggaa gtacaagttg
    14761 ttattacagc tccaagtagg gaactagctg cacagatcta tgaagcagct acacaaatcg
    14821 ctaaattttc tacaccggag atccgtgtct cgaattttgt cggcggtaca gacaaacagc
    14881 gtcaattaaa tcgtctgcat caccaacagc cacaggttgt agtaggcacg cctggtagaa
    14941 tattagacat gatgaatgaa caagcgctga agattcacac cgcatttgca tttgttgtcg
    15001 atgaagcgga tatgacattg gatatgggtt ttttaaatga agtagaccaa attgcaagtc
    15061 gattaccaga aaagctgcag tttttagttt tttctgccac tattccagaa aaacttcgtc
    15121 cattcttacg aaaatacatg gaaaatccaa tcattgaaga aatcaaacca aaagcagtta
    15181 tttctgaaga cattgaaaat tggctgattt caacaaaagg taaagatgag aatcaaatga
    15241 tttatcaact gctgacaatt ggtcatcctt atttagcaat cgtatttgcc aatacaaaac
    15301 agcatgtgga tgaaattgcc gattacttga aaaaccaagg cttgaaagta gcaaagattc
    15361 atggagacat caccccaaga gagagaaaac gagtgatgcg acaagtacag aatctggagt
    15421 atcaatatgt tgtagcgact gatttagctg ctagagggat cgatatcgaa ggtgtctctc
    15481 atgttattaa tgcagaaatt ccagaagatc tagacttctt tatccatcgt gtcggacgta
    15541 ctggacgtaa tggcttaaaa ggaacggcca ttacgttata ttcgccaaat gatgagcaag
    15601 ccattgatgc gatagaaaaa ttgggcgtct catttgaacc aaaagaaatc aaaaacgggg
    15661 aaatcgtcaa aacatacgat cgtaatagac gtgccaaacg agaaaaatca aaagatgtat
    15721 tagatcccac gctgattggt cttgtgaaga agaaaaagaa aaagatcaag cctggttata
    15781 agaaaaaaat cgattgggca attgcagaaa aaaataaaaa agaacgcaaa atcgaacgca
    15841 gacagcaaag ccgtactgct cgtaaaaata agaaaaattc acataaataa aaaacaagag
    15901 gttgtgacaa aaggcgtgtc acaatctctt gtttttaagc tagacttttt ttcattccga
    15961 tacaaagtac ttcatcttca ataaaaggtt ctgtgcaagt atgataccct tgtttttcat
    16021 aaaaatctac agccgtcatt tcagctacta cataactagt ctgacaacct tctgaaaccg
    16081 cccgtttttc agcagcttca agcaactgac cgcctaaccc ttttcctcgg tattcttttg
    16141 ctacgcaaaa tcgttccggc ttgaagcaag taggtgtttt cttttcataa cgcaaagtag
    16201 ctactggtct tttttgatca agaaatagat aataggtttg ttgcgggata tcttttccgt
    16261 cgaattctag ttctgcagga atcccttgtt ctaatacaaa aacttgatat ctcaaaaagc
    16321 agctagctgc gcgtatccat tcatccgctc caactatttt tttcatcata ttcctccttg
    16381 tcatgaatag cagagatgta ttgattcctt tattcttatc gtaccaaaaa tcgtggagta
    16441 tgaccaaaag gaaatgaaaa taaaaattga gatattgaca gtgatcaata gaatgcgtat
    16501 actaaataag tacgaaaagg atatgatttg ttttttaaga ctcacagaaa attctttctt
    16561 ggctgaaaaa agaatagtca ctaatcaaat tgctcccttt ttctattttg gtagaaatac
    16621 cgacgcagct cagcgttatc gagtttaaga ggtaatgatt acacatcatt gcaatcaagg
    16681 tggtaccgcg agttttcgtc cttggctgtg atggtgtttt tttgtattta atttatagaa
    16741 agcaggtaat gacatgaaac aattaagtag tgcacaagtt cgacaaatgt ttcttgattt
    16801 cttccaatct aaagaacata cgattgaacc tagtgcgtct ttgatccccg tgaacgatcc
    16861 aaccttattg tggatcaact ctggtgttgc aacattgaaa aaatattttg atggttcagt
    16921 tgtgccagaa aatccaagaa tcacaaatgc ccaaaaatct attcgaacaa atgatatcga
    16981 aaatgtcggt aaaacagcac ggcaccatac gatgtttgag atgctgggta atttttcgat
    17041 tggtgactat ttcaaaaatg aagccatcca ctgggcatgg gaatttttga ctgatccaaa
    17101 atggatggca ttcgatccag aaaaacttta tgtaacagtt tatccaaaag atacagaagc
    17161 aaaacgcatc tggcatgaag aagttggcct gccactagat cacattattg atgtagaaga
    17221 caacttctgg gatatcggtg caggcccatg tggtccggat acggaaattt tctatgaccg
    17281 tggagaagct tataacgatt tagcagaaga cgatcctgaa aactatccag gaggagaaaa
    17341 tgaacgttat ttagaaatct ggaacttagt attttcagaa ttcaaccata agcctgataa
    17401 cacatttgaa ccacttcctc ataaaaacat tgatacgggg atgggattgg aacgcatggt
    17461 ctcaatcgtt caagatgcgt caacgaactt tgagacagac ttgtttatgc ctattatcca
    17521 cgccgtagaa aagctaagca aacaagtaaa atatggtgaa aacccagtaa cagatatttc
    17581 ttacaaagta atcgcagatc atattcgcgc cctttcattt gctattggcg acggtgcatt
    17641 gccatcgaat gaagggagag ggtatgtctt acgccgtttg cttcgtcgtg ctgtcatgca
    17701 tgggaaaaaa ttaggtattg aacaagcatt cttatatcgt ttagtccctg ttgttgggga
    17761 aatcatggta agttattatc ctgaagtact tcaacaaaaa gaatttattg aaaaagttat
    17821 ccgtacagaa gaagaacgtt tcaatgaaac aatcaatgaa ggtttggaaa tcttaaatca
    17881 agtgatccaa gaagtaaaaa acgaaaacgg gacgactcta gcaggaaaag acatcttcaa
    17941 actatacgac acatatggct tcccggtaga attaacggaa gaggtggcag aagatgaagg
    18001 actgaaagtt gatcatgctg gttttgaaaa agaaatggat gcacaacgcg aacgtgctcg
    18061 ctctgcccgc agtactgaaa aatcaatggg ggtacaatct gctttattga cagatatcaa
    18121 agtagaaagt aaattcattg gatacacaga acaagaatca gatagtcaat tattggtcat
    18181 cgttaaaggt gagtctcttt tgaatgaagt cgcagaaggt gaagctcaaa tgatctttga
    18241 tcaaacacct ttctatgcag aaatgggtgg gcaagtagcg gatcaaggat ggatcatgaa
    18301 tgaagtaggc gaaatcgtag cagttgttac agatgttcaa aaagcgccta atggtcaatt
    18361 tatgcacacc gtagaagtaa aagcaccaat gagtgaagga caaacctatc atctattgat
    18421 cgatcaatcg atgcgcaatc ggatcatcaa aaaccataca gcaacccatc tactgcacaa
    18481 ggcattgaaa gaagtacttg gcgagcatgc aaatcaagct ggttctttag tcgctcctgg
    18541 acatttacga ttcgacttta cccactttgg tcaaatcact tcagatgaat tagctgaaat
    18601 ggaaagaatc gtcaacgaaa aaatctggga agctatccca gttgaaacaa ttgaaacaga
    18661 tatcgataca gccaaaaaca tgggtgcaat ggctttgttt ggtgaaaaat acggacatga
    18721 cgttcgagta gtaaatattg gcggctggtc aatcgaactg tgcggaggca cacatgtagc
    18781 aaatacagaa aacatcggaa tcttcaaaat cgtttctgaa tcaggaattg gtgcaggcgt
    18841 gcgacgaatc gaggctgtaa caagtaaaga agcgtacgaa ctacttgaag aagaagaaaa
    18901 acaactaaaa caagtagcaa caattgttaa atcacctcaa ttgaaagaag ttgtttcaaa
    18961 agcggaacaa ttgcaacagc aagtaagaga gctacaaaag gaaaatgaac aattagctag
    19021 caagttagcg aatcaacaag ctggtgatgt attcaaagat ccagaaacga tcaatggaaa
    19081 aacaatcatt gcagcacaag taaaagtgaa agatatgaat cagctgcgtc aattagctga
    19141 ccaatggaaa caaaaaaata tttcagatat tcttgtttta gcaaacgaac aagaaggaaa
    19201 agtcagtctg ctagcggcaa tgacgaaaga atcaaatgaa gctggactaa aagctggcga
    19261 cttgattaaa gcaatcgcac cgaaagtagg cggtggcggt ggtggacgtc cagacatggc
    19321 acaagctggc gggaaaaatc ctgctggtat tccagatgca cttaaagctg cgaaagaatg
    19381 ggttgcgcaa caatagggcg tttacgtttg acagaaaaac aaaagttcaa acattgaacg
    19441 aaacatgatt atgggggcga agacaaacaa gtcttgccct catacttttt tcaattggat
    19501 ggcgcatcta atcaaatggt cggattcgga tggtctttag ttgacgaaag tgacagaaat
    19561 ttttacgatt agtataaata aaaaggggtg aaaaaatgaa tagtagaaat aagacctttc
    19621 gactagtctt acgtgcgatc ttgttagcaa tcatcatcgt acaagcgatg gttccttggc
    19681 tggggtttat tccattagga ttcattagtt taacaattat ccatatcaca gtaattattg
    19741 ctgcagtagt attaggccca aaagatggca tggtgattgg cttgttttgg ggaatcgcta
    19801 caattgttcg tgcctatgcc atgccaacaa cgccatttga tacattagtg ttcacgaatc
    19861 cgattatctc ggtagttccc agagtacttg ttggcttagt agcaggcttg gttttccact
    19921 ggatttatca aaaaaataaa tcaataacga tcagctcagc ctttgcaggg gtactgggtt
    19981 cattggtcaa tacagtctta gtgttaggat tcatgggatt attttataca ggtgcaacgg
    20041 cagatgccta tggtgtagac tctgctcttt tatttaaaac cttagcaggg gttgcagcaa
    20101 tcaatgggat accagaagca ataggtgcgg gaatcataac accattacta gcaaaagcgt
    20161 tatttgcagc aacaccgttg aaaccagaat aatgaccatc ttttaatagt aataggacgc
    20221 acgatagata gaaaaaggaa gagaggagag gaaaacaatc gtcctctcca tctttttacc
    20281 tgagcaacaa gcaaactaga aggggtaaaa aacttggcta agaaacaaga agaaaaaaag
    20341 aaacaaatac cgcaacagcg gacaaaaaaa ggagtactag cgggtatctt aggattggtt
    20401 acgaatctga tcttgtttgt ctctaagcta atgatcgggc ttttttcagg aagtgtctcc
    20461 attatggctg atgcagtgaa cagtctatca gatactgctt catccatatt gactttgatc
    20521 ggatttaaaa ttgctgcaaa gccagcagat caggaacatc cttatggaca cgaacgcttc
    20581 gaatatatca gtgggttatt tgtttcgatc atcattactt atgtaggatt tcaattttta
    20641 gagtcttctg ctgaaaaaat catccatcca gaaaatgtct ctttaacacc catcgtcttt
    20701 tttgtcctaa tcatttccat ctttttgaaa tttgcacaag gcaaaatgta tcaggttatt
    20761 gcgcaatcca ttgattctga aacattgaga gctacagcaa cagatagtta taatgatgtc
    20821 tttactacac tggctgtatt gctttcagca gcaatcgaat gggtaactgg atggcgtatt
    20881 gatggatata taggttttgc tttagcatta tatatccttt atagtggatt gacgatgctt
    20941 cgaaattttg tatacgaact attaggaagt cgcccaacgc cagcagagat tgaagaaatg
    21001 gaagagcatt taaatgcttg cgatgatata cttggctatc atgatttatt ggtgcataac
    21061 tatggaccca ataaacgctt tgcatcagtc cacatcgaag tagatgaatc aatgaatttg
    21121 aatgtggctc ataagattat tgatctgata gaaaaagact ttaaaaagtc tttggatgtg
    21181 gaacttgtct gtcacttgga tccaatgcct gttgataacg aaaaatacta taatatcttg
    21241 cggcagttaa agcaaattct tcttgaaaca ggtacagggc ttaatattca tgattttaga
    21301 gtaattcatc aaggaaaaac gctgcaattt gatgtagtgg tccctgagaa attccagttt
    21361 gttgatacag aactagaaag aatcttacgt agtcaaatag aagaaaaaat cggagattac
    21421 caattggaaa ttacgtttg
//
LOCUS       DAJSKS010000037        20902 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJSKS010000037 DAJSKS010000000
VERSION     DAJSKS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.038"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            33..1372
                     /locus_tag="ON396_001180"
     CDS             join(33..474,474..1372)
                     /locus_tag="ON396_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HCR3113279.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
     gene            complement(1858..2487)
                     /gene="udk"
                     /locus_tag="ON396_001181"
     CDS             complement(1858..2487)
                     /gene="udk"
                     /locus_tag="ON396_001181"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR3113280.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(2571..3266)
                     /locus_tag="ON396_001182"
     CDS             complement(2571..3266)
                     /locus_tag="ON396_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCR3113281.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(3279..3995)
                     /locus_tag="ON396_001183"
     CDS             complement(3279..3995)
                     /locus_tag="ON396_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HCR3113282.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            4325..4930
                     /locus_tag="ON396_001184"
     CDS             4325..4930
                     /locus_tag="ON396_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCR3113283.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            5255..5578
                     /locus_tag="ON396_001185"
     CDS             5255..5578
                     /locus_tag="ON396_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCR3113284.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            5760..7130
                     /locus_tag="ON396_001186"
     CDS             5760..7130
                     /locus_tag="ON396_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCR3113285.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(7260..8561)
                     /locus_tag="ON396_001187"
     CDS             complement(7260..8561)
                     /locus_tag="ON396_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR3113286.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(8791..10749)
                     /locus_tag="ON396_001188"
     CDS             complement(8791..10749)
                     /locus_tag="ON396_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCR3113287.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(10926..11192)
                     /locus_tag="ON396_001189"
     CDS             complement(10926..11192)
                     /locus_tag="ON396_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3113288.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(11702..13828)
                     /gene="pnp"
                     /locus_tag="ON396_001190"
     CDS             complement(11702..13828)
                     /gene="pnp"
                     /locus_tag="ON396_001190"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR3113289.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(14016..14285)
                     /gene="rpsO"
                     /locus_tag="ON396_001191"
     CDS             complement(14016..14285)
                     /gene="rpsO"
                     /locus_tag="ON396_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR3113290.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            14536..15099
                     /locus_tag="ON396_001192"
     CDS             14536..15099
                     /locus_tag="ON396_001192"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR3113291.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(15352..15873)
                     /locus_tag="ON396_001193"
     CDS             complement(15352..15873)
                     /locus_tag="ON396_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HCR3113292.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(15876..16718)
                     /locus_tag="ON396_001194"
     CDS             complement(15876..16718)
                     /locus_tag="ON396_001194"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCR3113293.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(16711..17436)
                     /locus_tag="ON396_001195"
     CDS             complement(16711..17436)
                     /locus_tag="ON396_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR3113294.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            17612..17908
                     /locus_tag="ON396_001196"
     CDS             17612..17908
                     /locus_tag="ON396_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113295.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            18476..20068
                     /locus_tag="ON396_001197"
     CDS             18476..20068
                     /locus_tag="ON396_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR3113296.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            20081..20821
                     /locus_tag="ON396_001198"
     CDS             20081..20821
                     /locus_tag="ON396_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3113297.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         6159 a         4183 c         3945 g         6615 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agttcaactt
     1381 ttggggttca gttcattagg gggtgcgaca aaagagcttc ttcaaaaaaa taagtcgaaa
     1441 tctcacacaa attcgagaaa taattttcgt aagtttcttc ttatttattg catctgacca
     1501 cttctgttac aacctctcgt taaacggtgt tcaaaagctt gcatctacga taataagccc
     1561 aacccaacga aaaacgtgag gagctgtttt atttggatgc tcttatcgct tgatgcaaag
     1621 acagcttttt tacaacctct acttcacggt gctcaatcag ctgaccttcg gtattagctc
     1681 aaaaaacggt aaaatataaa cagctatttt ccgtttcttc tttttaatac ttaggtcaag
     1741 atgctgtttt cacaacctct tgttataaat aaaaaaaaca gttggctggt gactgttcaa
     1801 aaagcagaat ccttgctttt caaacggtcg cagtccgttc aactgtttcg ctgcgcacta
     1861 tttatggttt aagaaactag ctacttttgt tgtaatcaaa tcaatagcaa cgtggttttc
     1921 tccgccttct ggaacaatga tatccgcata gcgcttcgtt ggttcgataa attgatgata
     1981 catcggttta acaactgtca aatattgttc gatcacggag tccaatgtgc gtccccgttc
     2041 ttccatatcc cgtttgatac gacgaatgat acgaatgtca tcatctgtat ctacgtatac
     2101 tttgatatcc atcaagtcac gcagacgttc gtcttctaaa atcaggattc cttctagaat
     2161 gatcacttct tttggttcct gaatgatcgt tgcctgactt cttgtatgcg ctacatagtc
     2221 atacacgggt ttttcaatgg ctttatagtt cagtaagtcg cctacatgct gaatcagcag
     2281 atcattatca aaggcaaatg ggtgatcgta atttgtgttc aatcgttctt caaaactcaa
     2341 atggctctga tccttgtaat aagaatcttg ttcaagcatc atgatggaat ggtccgggaa
     2401 gttgttgaaa atcgcacggc tgacacttgt ctttccgctt ccggatccac ccgtcacacc
     2461 aatgataatt ggcttgcttt ttgtcattga ctaaaacctc tttcgtatcc gttttttcat
     2521 tctaatctat tatagcgaaa aaacacgaaa atgctatatt tttctgaaaa ttaatccttt
     2581 tcaatttcca ataatgaata aagttcctct aatgaatcga ttcgataagt aggctggatt
     2641 tcttcgattt ctggtaattt atttctattg aaccaaatcg tatcgatccc tgccatattt
     2701 cctcctttga tatcagaagt caaagaatca ccaatgatca cagtttgctt tttatcaaaa
     2761 ttaggaatgt gggaaaagac atgatcaaaa aattctttca tcggcttttg cgctccgaca
     2821 gcttctgaga caaagatatc cttgaagtaa tcaagcattc ctgcatctgt taatcgccga
     2881 tgttgcgtgt gcgacacacc gttcgtcaca atataaagat cagcatgcgg atggaccttt
     2941 ttcaggattt ctagacttcc tggaacaaga tcatgacctt ggttcaagta gtggcggtag
     3001 cgtttttcca tttgatcact atctactgtt ttcccaaatt gctggaaaag taacccaaaa
     3061 cgactgccta ttatttcttc tcgtgatagt ctgccttttt caaactcctg ccataaatga
     3121 tgattcaaag ccttatagct ggtttcgatt tctggtgtca atgtgattcc ttgttctgca
     3181 aacaaagcac ttaaagcatt ggcttcagtt aattgaaaat ctaacaatgt atcatctaca
     3241 tcgaacaata aagttttata tttcatcttt ttgacctcct atttaaaata aatacttaac
     3301 tttttcatct ctgctaatgt caacgatcga tactcgccta ctcctagctg ttcatctaat
     3361 tccaatggtc ccatgcttag ccgtttcaat gcggtcactt tctttccttg ggctaagaac
     3421 atctttttga cttgatggaa ttttccttct tggatcgtca ataaaaccgt actttggtgc
     3481 ttgtctgctt tgagtatttt taaatcagcc ggcttacatt gatacccatc agcgaatacg
     3541 attccatttt caaatgctgt tacgtctgcc ggtgtcaccc tttcgttcac aactacttga
     3601 taagtctttg aaaccttttt ttctggaagt agcatttcat atcccaattg tccattcgaa
     3661 gtgatcagca atagtcctgt tgtatctcta tctagtcttc ccactggata taatggttgg
     3721 aaacgatcgg cacttgcaat cagatcgatt actgtcgtct tttctggatc tgatacagct
     3781 gtaacagctt tttcaggttt attgagaaga taataggact catttgtttc taagtgttca
     3841 cctgctactt cgatttgatg cagtaaacta tctacatttc gattttgtcg aaattcgact
     3901 gtgccatcta tcatcacttg cttcaaagca aacagccgct tcatttcctt gcgtgtcgtt
     3961 tctaattttt cttctattaa tttatctaaa cgcatgctgt ttcctttctt attactacta
     4021 attaattata ccttgaatat tcttgcttgc aaatacatca tcatagttat tccttgactt
     4081 aattaacagt tcacgttacg gttaaatagt aaagtgaatt ggatttctca tacacagcgt
     4141 aatgacctgg tggagaaaag ttgaatcctg actttctaag gggagatgcg cgcatctctc
     4201 cttttgcgct tttttaagcg ccctgccttc gatgatgtgc acgagattag cctttagaat
     4261 tcacttaaat aaaatctgtc cgttaccaaa gcagacagtc acattttaag gaggaatttt
     4321 ggttatgtca gtatcattag aagtaagtaa aagagaagta cgtccccgtt cattaagaaa
     4381 caaacttcgt cacgaaggga aagttccagc tgttgtttat gggtataaag tagaaagtac
     4441 ccctatctca ttcgatggtt tgacactaag caaattgttg cgtgaacatg gagcgaacac
     4501 agtcatcacg atggacatcg atggacaaaa agtcaataca ttggttcaca agatccaaac
     4561 gaacactttt accaatcatt tcgaacatgc agaatttcta tctgtaaaca tgtcggaaga
     4621 aacagaagtc gaaacagaag tcgttcttgt tggcgaatca gtgggtgcta aatctggtgg
     4681 agtgttagct caaaacttgt atacagtagt cgtatctgct acaccagaca aattgcctga
     4741 acgaattgaa gttgacgtga caaacttgga attaggtgat tctattacag tagccgatct
     4801 tccaaaaaac gacgattaca aattcgtgac cgacagtgaa gagcaaatcg ctgctgtagt
     4861 cgctcaaagt gaagaagaaa gcacaggtga aggtggcgaa ccagaagtga tcggttcaac
     4921 agaagaataa tactgaaaca atcaggtggt gttagaagcg gcgggtcagc agcttttaac
     4981 acgccttttt tatttctaat cgtttatttt catatggggc ttccactttt gtaaatggct
     5041 ttacaaacga aaaatcctaa tttaccaagc ttttcctcgc atacttttat cgtaccttta
     5101 taaaaattta tactttgaaa aaaagtattc aattattgtt gcatttttcc tgattcttta
     5161 ttagaatgaa ccctcgttga agtaagtatt tctagtttat cctaggatta attataaaaa
     5221 taaaacattc gttgattcga aggaggttaa acagatgttt gaaagttttg atagcagccg
     5281 caaccgttac gcttcagttg gtgtcgtatc aagtttgcca gatgaattga ttgacagtat
     5341 atggtttatt attgacctag atcttaaggg agtcattcca ttagaaaata tattaacctt
     5401 tgatttgatc gaaaatcgtg gaaaagtgac gatgcatttt tctcaagcag gaagcaatgt
     5461 ggaaatggat attgatttgc cgtttgccta ttcgaaccaa tttcctgctc aagtatacgc
     5521 ctatgatgac ggtacgagag aaacgatcct tttaccaagt gagattagac aatattaagg
     5581 tgatagataa acgtggatgt cccttatttt acagaccagg ccaagttgtt gattctttga
     5641 ctcaattctt ggcttctttt tatggagttt cataaattat cattgatttc taattggtat
     5701 tctcaggata aacgctatat actactaact aaattttaaa gatataagga gtttaaaaga
     5761 tgcgcgattt aacaaaagga agccccgcta aactgatttt gatgttcact gttcctttac
     5821 tgatcggaaa tgtttttcag cagttctaca atatgatcga tatgatcatc gtgggtcaga
     5881 cgctaggtaa aaatgcgctg gcagcggttg gtgcaactgg aagcctgaca tttttgatca
     5941 ttggctttgc ccaaggttta acagctggtt tagccattat taccgcacaa cgttacggtg
     6001 caaaagatta tcggggtctg aagaaaagtt ttgctgctag tgttgtcatt agtcttgtcg
     6061 tcactgtgat tttaactgtc ctaagtctcc tgtttattcg tccgatgctt caattgatgc
     6121 aaactcctcc tgaaatactt gatcaagcac aaacatttat ttcaattatt ttattaggga
     6181 tctttgcatc agtcagtttc aatttgcttt ctaatgtcat tcgcgcactt ggtgacagcc
     6241 gcacaccact attctttctg atcattgcgg tcatcatcaa cgtcgtactt gatttgatct
     6301 ttattatcta ttttggcatg ggtgtggaag gtgcagctat tgctacagta atcgcacaag
     6361 tttcttccag cgtactgtgt ctagtgtata tcaagaaaaa aatgcctttg ttacagttac
     6421 gcaaaaaaga tttcaaattt gaaaaagaag agtttgctgt acacctaaat gctgctttgc
     6481 caatggcgtt tcaatcttcg atcatcgctg ttggtgctat cgtgttacaa gcagcactaa
     6541 atagtctagg gacagatgtc gtagctgctc aagccgctgc tggtcgtatc gaccaatttg
     6601 caaatcagcc gatgatgtca tttggtattg caatggcaac tttcactgca caaaactatg
     6661 gtgcgaaaga atatggccga atcttgcaag gggtacgtca aactttactg atgagtgtcg
     6721 gatttagtct cttagcaggt gctatcgtca ttttcttcgg tcatttcttt gttcaattgt
     6781 ttgttagccc atcagaaaca cgtgtatttg aattagcaca aacctacttc aatatcaatg
     6841 ggtctcttta ctggatctta gcagtcctgt tcattttacg ctacacactt caagggcttg
     6901 gacaaagcaa gatccctacg atcgccggta tgatggaatt gcttatgcgt tcttttgcag
     6961 ctatcgttct tacaggaatg tggggatacc caggagcagc agcagcttct cctttagctt
     7021 ggatcggttc tgttgccgtg ttgctttact cttatatgcg ttcgatgaaa caattgaaac
     7081 acatgggtga agaggaacat ttcacattag aacatgcaga agcaagatag ccttgattaa
     7141 aagaaatcgt tgcggctaaa aatgaaaact tttattgaaa atatcttctt cgtttaccga
     7201 aaacaggttt tttagataac aaaaaggctg tgacaaaaaa tgtcacagcc ttttttcttt
     7261 tattctattt ttctttccgt ggactctact ttgaaataat cgatatgtgc atgctggtat
     7321 tctaataccg tgaaacggaa tgatgcaatc tcaatgattg ttcctgcttt tatttcttta
     7381 tatcggctag aagcaaagcc cgcaagtgta gtaaaattcg tttcttcaaa ctctggaatc
     7441 tctacatgaa aataacgctg gaaatcatct aatgttagtt tgccggagat gatataagag
     7501 ccatctgact gttccgctat caatgcttcg tccaaatctg tctcatctcg gatttccccg
     7561 acgatttctt ctaaaatatc ttcaatcgtc actaagccgc ttgtccctcc gtattcatct
     7621 tttaagatag caatatgctt tccctttttt cgcatgatcg tcaataattt ttttataggg
     7681 atcgtttcag ttgttacgat cggctcttca atcaactgtt ctactgtcag ctggtcatct
     7741 ttataagatt gcttgatcaa atctggcaag gtgacataac ctaaaacatt gtcttttgat
     7801 tctaagataa ccggatagcg agtatgtcct tgttcaatcg aagcttttat cgcttctttg
     7861 acggtttctg aagcttcgaa tacttgcatg gacgtacggt ctacttgtat atcttttgcc
     7921 aaggtatcat caaattcaaa cacgttttcc aaataatgat attcttcttt ggtcaactct
     7981 cctttttcat aagaattcaa tgcgactaat gtcagctctt cttgagacaa tgtctcttct
     8041 ccttcagata cgagacgcac acctaatagc ttccctagcc ctgcagctga atgattcaat
     8101 acccaaataa aaggaaacat tactttataa aaataatgaa gtggtttaac tacgaacaag
     8161 acgaccttct ccgtattgac gatactatag ctcttaggaa gcagttcgcc aataaccaca
     8221 tcgatatacg taactaaaat aaatcccagc gccacagaga ttgtactgct aatagccgaa
     8281 ctaaaaggta gtttaccgat cactggttct agtaaagccg cgacagatcc ttcaccgacc
     8341 cacccgatga tcaaaccggt tagcgtaatt cctaattgac tagctgataa atagttatct
     8401 aaatggtgtg taacatgaag agctagcttg gcgttcttta cattttcctt ttctagttgt
     8461 tccaatcttg atcgtctgat tttcactaaa gcaaattcac ttgctacaaa aaatgaagta
     8521 attgcaatca tcaaaaataa aataatgaaa ttgataacca tattctcccg cctttctctt
     8581 tttctattgt accctttttc tacaaaaaga ttgaaaataa tgcgtattca tatttaggaa
     8641 taaaaaagga gatgcggcat ttttcagcca catctcctta tcacttcaaa atcattgcgt
     8701 tacttgttgc taaacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat
     8761 tcgacattag ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg
     8821 agtatttcca atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa
     8881 ccatgtttct ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg
     8941 ttgccatttt cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa
     9001 atcgccaact tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc
     9061 ttgtttcatc aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct
     9121 gaaacctaag aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata
     9181 ttctttcgta tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct
     9241 tttaggccgt ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat
     9301 atcaggaacc aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa
     9361 ttctctaagc tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat
     9421 aatgttccct tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc
     9481 tacaaaaccg cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc
     9541 accgaagaaa aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata
     9601 aaagtaaagc agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc
     9661 ttccatatga tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat
     9721 cagattgact gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa
     9781 ccgaggcagc agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa
     9841 tgcttgcgaa gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact
     9901 ttgaggcatc atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg
     9961 ataaacattc aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac
    10021 gtaaggccag ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc
    10081 ctctgcacct gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc
    10141 tgcactaaaa agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc
    10201 ttgactgaag ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg
    10261 tccgaatgct tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat
    10321 aataactaaa gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg
    10381 cggaattcct cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc
    10441 atctgctagt aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt
    10501 tcggacaagt gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc
    10561 aatcacgaca tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga
    10621 tccgataaca aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt
    10681 cataacgtaa agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag
    10741 gccagccatt gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt
    10801 gttatatgga tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc
    10861 cgctttccta aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa
    10921 taaaattatt ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca
    10981 aggcaaaaaa ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc
    11041 agtggtccta gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca
    11101 gatacgcctc ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg
    11161 ccgataaata acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct
    11221 cacttttatt ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga
    11281 aaaaaagaag ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac
    11341 cgtgaatcag aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa
    11401 aatagttcct catattttgc cgttttttga gctaatatcg agggacagct gattgaacat
    11461 cgtttattgg agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaagta
    11521 aaaacagctc ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga
    11581 acaccgttta ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc
    11641 gaataattat tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa
    11701 attatttttc ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat
    11761 ttacgcgtcc ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa
    11821 caacatcttc gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat
    11881 cttttccttt gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta
    11941 gataaacttc accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt
    12001 tgatcatttc tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt
    12061 tcacaccagt ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt
    12121 tgattttagc aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt
    12181 cacgtggagc agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg
    12241 cttgagttaa tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct
    12301 gtaaagcagt gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat
    12361 cttccagccc ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca
    12421 tcgcaatacc ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc
    12481 cagcacagat acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac
    12541 ggatcgtata aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta
    12601 atgcgccatg tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag
    12661 aaaattgagg gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat
    12721 caatgatttg gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc
    12781 ctcgtgtgaa caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg
    12841 gacggatttc atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac
    12901 gtacgacatc tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt
    12961 catcttcatg acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct
    13021 cgatattatc ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt
    13081 aagaagcatc aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca
    13141 ttttttcttt ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag
    13201 cgtcaaaacc aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc
    13261 tttcaaccat attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt
    13321 ctgcttgttc tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc
    13381 ctgcgattgg gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca
    13441 ttgctgccat agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga
    13501 cttcattacg gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag
    13561 tcaatgttgc ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc
    13621 ctgctgcata cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg
    13681 cttctttaga ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg
    13741 ctccgtttgc ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg
    13801 tcgttttgaa tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga
    13861 cgctactaaa caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag
    13921 taggagtcca tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt
    13981 gagattcatg tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg
    14041 atcaattcgc ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg
    14101 tgaccaactt ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca
    14161 tgttcattca agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca
    14221 gtatcccctt catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt
    14281 gccatttctt tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg
    14341 cttaaccaag tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg
    14401 ttgaaaatca agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact
    14461 tttccatttt tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa
    14521 aggggtacat attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta
    14581 cgcgaagttg caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa
    14641 gagatgcttg agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta
    14701 cgaggcggtg ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt
    14761 cacgtaccaa gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat
    14821 cctaaaattt tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta
    14881 tctgttgatc gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat
    14941 tatgatatga atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt
    15001 caacatgaaa tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat
    15061 ccatttgcac taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga
    15121 tactatgcta caagaaagct ttataaaaaa ggggagtgct caaaagctgc acttcccttt
    15181 tttatgtttc tccactaatc gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa
    15241 ttctgtgtat tggatacgca tcttttctta ttttttctta tttcttccat atgggtgttt
    15301 atctcagaga tgatagaata gtttctagga taaaaagtag cagctacgat gttatccttc
    15361 gtagaggatt cctgcttcag ctagtttttg tttgatgttt ttcagcatat gcttttctgg
    15421 gtagcggatc gtatgcagat ggatgccgcc tgttaattcg gataacaaag tagcattact
    15481 tttttgataa cgtctcataa aggaatcgat atcttgtggc gttttgatgt gcaaagttcc
    15541 tgctaactca ccgtaaaggg gatgttccac aattacatca atgatttcac caccattttc
    15601 aacgattagt ctcagttctt cttctgtttg atcttttcca tgctggacag cgattttact
    15661 gatgtgccca gtattggttt cttctgtttc gaggatataa cctcttgctg ttgccacgat
    15721 acttttacct gcagctcgta gtaaagcaat atcgcctaca atgatctgac ggctgactcc
    15781 aaaagttttc gcaaacttgc tagcactcac tggtttttct gtatttgcca aggtttgcat
    15841 gatttgtttt cgccgtgtct ctccttcgat cacacttatt cctccttcat tttgttttgg
    15901 tattcgcgcg agtgtttcat caaatcaaca tacgtgtctg ttattgtttc cttatatccc
    15961 ttatttttca gcagactaac aactttatcc aatacttgct gctctctttt tgcatcataa
    16021 actgcttggt tttcttcttt tttcacttgt cctattttag ttaccgtatc cattcttttt
    16081 tctaataaag caaccaactc tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga
    16141 gactcttctg ttagttcttc ctctactgtc ttgatgatac gagcaggatt tcctgcaatc
    16201 actacattgt cgggaaaaga tttggtcaca accgaacctg cgccgactac tacgttgtcc
    16261 cctagagtga cacccggcgt gacgataact cctccgccta accaaacatt attaccgatt
    16321 ttaatgggtt ttgcgtactc cagaccgctg ttacgtttca ctggatgtag tggatgcgtc
    16381 gccgtgtaaa gctggacatt cggagcgaac atgcagttat caccgatttc aatcgtgctg
    16441 acatctaaaa atgtacaatt gaaatttgca taaaaattct ccccaacaaa tatattatat
    16501 ccgtaatcaa aattgataac tggctccata tagatttttt tccctgtacg gccaaaagtc
    16561 tctttcaaca attgggatcg cagttccgct gattctgctt ggttgatgat ttggctttga
    16621 cttcttgcaa acatacggtc ggcaaccagc tcaggatcat tggcaaaata aagctcgcct
    16681 gcaatcattt tttctttttc ggttttgaat tcagtcataa tctgctccta attcttgtat
    16741 ttttttgaaa acaaccgctc cgtcttcttg aataaacgta taaggctccg ctaaataaac
    16801 aggcgtttga ttgattacta agattttggc tgacggctgg cgaaactgta ccagatcaca
    16861 aaacggatga acttgaaaac tcgttccaac aatgacgatc agctctgctt ggctgacggc
    16921 ataagccgcc tgctccatga ctccatcttg aaacccttca ccatataaca cgatgtctgg
    16981 gcgtatttgt cctccgcatg cttgatgttt gtcgctgtga agatattctt tccagtctac
    17041 cgtttgttga cattttcgac aatgacattg atacagactc ccatgaaaat tgacgagttg
    17101 cgggctttta gcctttgcgt gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt
    17161 ttctttcgct agatgtgcta tttccaaatg aatgatattg ggttttgctt caggatgata
    17221 aatctttttg acaaattgat agaatttatc cggttcattc accattgctt gatgactcag
    17281 caagtattcc ggctgctcca tcccgtggta gattcctgac aaagatcgat agtctggcac
    17341 tccagaaggt gtggataccc ctgcaccggt caaaaatgta atttgctgat aagctaatat
    17401 ctcttttgct gcttcgtcta ctgttaattg tttcatgttt tgtcacctca ctttccatta
    17461 tagcccatac tcgaacaaat ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt
    17521 tatgggcatt tgagcaattt atcacaatgt tcactgacaa aagaatgaaa acgtgttaca
    17581 atactgctgt ataaaagtga ggtgaatcga tatgttacca ttctttattt attggggcgt
    17641 tattctggct tgtatcgcct ggttagcgtt aagcgtttat ttctctgttt tttatcttgt
    17701 acgcaaagaa aatggaaatc tttgggcttt tgctttattc aacgtgattg ccgcaattgt
    17761 tttagccatt acattagccg tatatcgaac atggggctgg ggaatcaccc aatactcaag
    17821 tttgatttat ctaatattag ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg
    17881 acgtgagcca aagaaagcgg cagcttaaat gatcgagatg gaactggaca caggaaattt
    17941 tctgttctag ttcttttttt attgtattac atacattttt gtgtataatt atactatcgt
    18001 ttgcaatgga tagatgattc acattttgat tgttttttcg gtaaaagtaa agatttctat
    18061 atttatcagt attttttcaa aaaccagcag ttatagattg acaaagcctc tcgagtatcg
    18121 taaacttttt ttgtataaat aactgaatac gagatgcctc aatcaatgag gtagaggtcg
    18181 cgagaatcat taaccctgtg gagtgaagca aacatggaga agcaggaata gggaggatcg
    18241 ccgaaatgtg cacttttttg cttggagtgt atgttgggac gcaagttaag agcttgcgga
    18301 ctgtcttagc agtgatgcta agttgcgcta tgttttgtga gaggtttatc accttatgga
    18361 attttagcgg cctttttata tctataaatc ggtcgctttt tattttttca gataaagctt
    18421 taccgcattt catctagacc gctcgtgtat catataatca gaaagggaga aaaaaatgaa
    18481 aaaaaatatg gtttcagttg ccgtactatt tgcttttgtc ttctcactat tcagtgtggg
    18541 atctctggca aatgcagatg aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc
    18601 tgggtatgca cccttcaact ggtctcaaca aacagatgag aataatgcct tgccaattca
    18661 aggccaaaat agttacgccg gcggttatga tgttcaaatc gctaaaaaag tagcagatgg
    18721 tttagggaaa aaattagtca tcgtccaaac gaaatgggat ggtctggctc ctgcccttca
    18781 atcaggcaag atcgacgcga tcatcgccgg aatgagccct actgctgaac gacgaaaaga
    18841 aattgatttt accgatcctt attacgaatc acaattagta gtagtagttc aaaaagatag
    18901 caaatatgca aaagcaaaaa gtttagcaga tctttctgga gcaaagatca ctgctcagtt
    18961 aaatacattc cactatactg tgatcgatca gataccagat gtgcagaaac aacaagcaat
    19021 ggataatttc tcagcaatga gaacagcttt agcttctgga atgatcgatg gttatgtcag
    19081 cgaacgtccg gaaggtgtaa cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt
    19141 cagcggtgca gatggcttcc agacaaaccc ggaagatgtt cagatttctg ttggtatgcg
    19201 taaaggcgac ccggatatcc aaaaagtaaa ccagattctt tctggcatct ctaacgatga
    19261 acgaaccaaa attatggacc aagcagtcaa agatcagcct gctgcaactg atagtgaaga
    19321 aaaacctggc atcatttctg atttcactac gatttggaaa caatacggca atatgttctt
    19381 acgaggtgct ggacttacct tgttcatcgc attgatcggg acagttgtcg gtactacact
    19441 tggattactg attggtgtct tccgttcggt acctgaatca ggaaatgcac tgactcgctt
    19501 cttccaaaaa atcggaaatg cacttctttc tatctatgtc gaagtattcc gcggaacacc
    19561 gatgatggta caagcaatgg tcatcttcta cggtctggca ctcgcttttg gcatttcatt
    19621 gaaccgaacg attgccgctt tgtttattgt ctctctcaat actggagctt acatgacaga
    19681 gatcgttcgt ggggggattt ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat
    19741 tggtatgacc catggccaaa cgatgagaaa agttgttatt ccacaagttt tacggaatat
    19801 cttgccagca acaggaaatg agtttgttat caatatcaaa gatacagcag ttttaagtgt
    19861 aatcggcgta gctgatttgt tcttccaagg aaactctgct tccggtgcga atttccaatt
    19921 cttccaaaca tttacgatcg tcgggatcat ctacttagtc atgaccttta cgatcaccag
    19981 aatcctgcgg gcggtagaaa agaaaatcga tggtccttca gcttatacca agcttgaaga
    20041 aatcgacaat tcaaatttgc aggaataagg aggaagaatc atgaatgaaa aaattattga
    20101 aatcaaacat ctaagaaaaa gcttcggtga aaatgaagta ttgaaagata tcagcgtaac
    20161 agtcaataaa ggtgaagttg tgacgatcat cggttcttcc ggttctggta aatctacttt
    20221 cttgcgctgt atcaatttac tggaaaaacc aacaggcggc gagatcattt ataatggaga
    20281 aaatgtatta gctcctaaat ataatttacc taaatatcga acgaatctag gtatggtttt
    20341 ccaatccttc aacctgttca acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac
    20401 aactgtcctg aaacgagata aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa
    20461 agttggtatg gatcgttata tcgaagccaa accagcacaa ttatctggtg gacagaaaca
    20521 gcgtgtcgca attgctcggg cattatcaat gaatccagat gtattgctat tcgacgaacc
    20581 tacttcggcg ttagaccctg aaatggttgg cgaagtgctt aatacgatca aagacttagc
    20641 acatactggc ttgacaatgc ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc
    20701 tgaccgtgtc atttttatgg ataaaggtgt gattgcagaa gaaggtacac cagaagatat
    20761 ctttgtccat ccgaaagaag aacgtacaaa agaattcctt tcacgtattt taaatgctta
    20821 ggatcaatag ctgattggga caaagagctg cggtgtttac cgcagttctt ttttatgaat
    20881 tactcataaa aggtaaattt ct
//
LOCUS       DAJSKS010000038        20897 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJSKS010000038 DAJSKS010000000
VERSION     DAJSKS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.039"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..438)
                     /locus_tag="ON396_001199"
     CDS             complement(<1..438)
                     /locus_tag="ON396_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCR3113298.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIG"
     gene            complement(625..1275)
                     /locus_tag="ON396_001200"
     CDS             complement(625..1275)
                     /locus_tag="ON396_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113299.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1818..2753)
                     /gene="whiA"
                     /locus_tag="ON396_001201"
     CDS             complement(1818..2753)
                     /gene="whiA"
                     /locus_tag="ON396_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCR3113300.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2787..3785)
                     /locus_tag="ON396_001202"
     CDS             complement(2787..3785)
                     /locus_tag="ON396_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCR3113301.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3782..4666)
                     /gene="rapZ"
                     /locus_tag="ON396_001203"
     CDS             complement(3782..4666)
                     /gene="rapZ"
                     /locus_tag="ON396_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR3113302.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4801..5532)
                     /locus_tag="ON396_001204"
     CDS             complement(4801..5532)
                     /locus_tag="ON396_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HCR3113303.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5536..6801)
                     /locus_tag="ON396_001205"
     CDS             complement(5536..6801)
                     /locus_tag="ON396_001205"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HCR3113304.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7064..9880)
                     /gene="uvrA"
                     /locus_tag="ON396_001206"
     CDS             complement(7064..9880)
                     /gene="uvrA"
                     /locus_tag="ON396_001206"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR3113305.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(9890..11884)
                     /gene="uvrB"
                     /locus_tag="ON396_001207"
     CDS             complement(9890..11884)
                     /gene="uvrB"
                     /locus_tag="ON396_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCR3113306.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12143..13645
                     /locus_tag="ON396_001208"
     CDS             12143..13645
                     /locus_tag="ON396_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HCR3113307.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13647..14384
                     /locus_tag="ON396_001209"
     CDS             13647..14384
                     /locus_tag="ON396_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3113308.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14555..15748)
                     /locus_tag="ON396_001210"
     CDS             complement(14555..15748)
                     /locus_tag="ON396_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3113309.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15827..16033)
                     /locus_tag="ON396_001211"
     CDS             complement(15827..16033)
                     /locus_tag="ON396_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR3113310.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16026..17168)
                     /locus_tag="ON396_001212"
     CDS             complement(16026..17168)
                     /locus_tag="ON396_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR3113311.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17680..18315
                     /locus_tag="ON396_001213"
     CDS             17680..18315
                     /locus_tag="ON396_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3113312.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18469..18546
                     /locus_tag="ON396_001214"
                     /pseudo
     CDS             18469..18546
                     /locus_tag="ON396_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18791..18907
                     /locus_tag="ON396_001215"
     CDS             18791..18907
                     /locus_tag="ON396_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCR3113313.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            18923..20842
                     /gene="tet(M)"
                     /locus_tag="ON396_001216"
     CDS             18923..20842
                     /gene="tet(M)"
                     /locus_tag="ON396_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR3113314.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT         6136 a         4279 c         3719 g         6763 t
ORIGIN      
        1 accgattagt gttaaaatat ctttattctt cttgctaaca taacccttta tgcacaaaag
       61 aacagctata cttaaagtaa caaataaata acgtattcca atagaaaaat cactataaat
      121 actctctagc ataggaccga caattgaaga agtcactact gcaaatgctg gacctatcca
      181 agcaacatta gaatgttctt tctctctttt cctttgtaaa tctttgttga gtattaatct
      241 atttatttct tctgcaggca agttaatttt cataacctta tgggggatta tccaattaaa
      301 aatcggaaaa aggtagcctc cccaaaaagt atagtgatct gtgtcaatca gataataacc
      361 tgattttgat tccagaataa catatctcaa agccgaacga aaatttttca tgaatatcca
      421 taatctcact gtttccacac ctaattcctc caattccaat taaatggatt tatagcatca
      481 ccaattatgt taactgcctg accaacctca ttgataaaat tggcaccact atcccatgct
      541 tcaacaatgc tatatatcta tcaaacaaaa aatggaaaac taaatactcg cgcacataaa
      601 ttttacattc tgttttttat aagactaata ataatcattg aaaaaatttc ttatcggtgg
      661 cgcatattcc caattgatta atgtgatccc atattctgca agtattttac gagcttcttc
      721 taagtcatag tgagaaaaat gcataaaacc tgcaatacga tcacggactg tgagctgtgg
      781 cttgcttcca ccatcattca taaaatatcc agcagagcca ctacttaaag tgattgttac
      841 atcttcactt tctattatta agaattcata atgaaatgct ttcaactctc cactattatt
      901 taaaagtttt atactccaac ttcgtctagg ttgagttgtc acattccctc taaaacttcc
      961 tataaattct gcaatcaccg tatcttcaat caaaattctt tcctcatcta tttcatattc
     1021 aagactaaac ggaaattctc catatgtaat ctctggcaat ggtggtgctg tagttctcca
     1081 aataaataca ataactgtga atagaaaaaa tccaacgatc gatataaatg ttatgagacc
     1141 tgctcgaagt aaaacccgat ataataaaaa tgctgtattt cgctttgcta tatctagaaa
     1201 aataaaaagt actgcaatca taatgatagt aaaagttgta aaaaagcaca ttttttgccc
     1261 tccctttata aacatcgatt ctcaaattct aaaccaaatc ttgatagttc aagttcaggt
     1321 ataacgtata ccaaccatac atcccaatta ctatttatga acttatgatt atcactgaat
     1381 aaatcactcc atccccttgt taaaacttct acattaatta cacctggttg ttttcgacta
     1441 gctaatccaa tgaacttatc aaccatcctt atccaccaac cacgagaatc ttgaccagaa
     1501 gtaagaaaaa cagttctcaa ggttgatcga gcagttcctt ccagttttta attaatctcc
     1561 agttatagat aaatacttga tcctatctat atcaggactt ctatattaag caaaagttta
     1621 tgtataaaac taaaatcttc attcagttgt ctccccgctt tttatacgat tgtattaaaa
     1681 aacaatagaa gcctttcaac attatgttac tccagtaaag ttggaattat agtttgacgg
     1741 aagaaaagca agtatagctt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta
     1801 tcagtttttt ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt
     1861 ttgcgaattc ggtgattaat tcccgatttt gatattgcac ctgagggaat catttcccct
     1921 aattctttca agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt
     1981 tcaggaaggg catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta
     2041 gaagccgcat caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc
     2101 gaatttctca tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca
     2161 ccaatcaatg ttaagaaatc agcaattttt tctgctcctt ttaaatacga aatatagcca
     2221 ttgcgccgtt caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct
     2281 tggttatgct gttcataaat agaaaatatt tccaaatgat aacgactcgt ttccggatta
     2341 ttgactgaac ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt
     2401 cccataatct cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc
     2461 atcaacacat cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat
     2521 ttcatttttt tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa
     2581 tagatacgtc tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc
     2641 acaataccta aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct
     2701 cgatgaactt ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc
     2761 tcctcctttc tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat
     2821 tcttctacta ctttgtctcc atcgtggaag acaccaccat ctttcaattc taaaaagtcg
     2881 gtggaaatca ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact
     2941 agatactcat catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc
     3001 aatactgtat cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca
     3061 tctgtgaaat gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca
     3121 gcagttgtct tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg
     3181 gtgaataaac ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact
     3241 tttcgagccg ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg
     3301 gttttacggt tgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaaaaca
     3361 gcatgcaagg tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt
     3421 tttgctaata attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg
     3481 atcagattac cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg
     3541 aaaatatctt catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca
     3601 ccaggaggag ccatagcgat cgattctctg attgcaccgc tgcttccacc atcatctgca
     3661 acagtcacta ctgctgtgat atctgctcct tgatttcgta aacttttcag gataactggc
     3721 agaccagtgc caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt
     3781 ttcacgaccg gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt
     3841 tatagttttt ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac
     3901 cgcctgtaca gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca
     3961 atacagtttc cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca
     4021 tcacataatc atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat
     4081 gagggttggg caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt
     4141 taaaaccaaa tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact
     4201 cattgatttt ttcacgtaat tgtcgtggtg tcaatgttgt cgtatcaatg atcacggaag
     4261 cttgggcttt caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac
     4321 cgtccatcgc cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt
     4381 ctgaagcatc taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca
     4441 gcatgttttg gatttcttca aagaaggaac gagaacgcaa atccactcct aaagccattt
     4501 ttgtgacttt acctgattct ttgatcaatt cccagaattt tggaatcaat cgaggcggca
     4561 tattatcgat acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg
     4621 caccactcat acctgttatg atgactaatt gcaaattatc tgtcatattt acgaacccct
     4681 ctcacattgt gcataataaa attatatcat cccgggcgta tcattaccag ttttcttttt
     4741 cttttacaaa aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt
     4801 ttacttttcc gatgctgtat ccaatgcctc gcttctttca gctaatgcac gttcgatcgt
     4861 tcgatccgct aaaatagatt gagcatcgat cactgggtaa tgattatctt cagcaaattc
     4921 atgcatcaaa gataattctg tgcaacctag gatgactttt tcacagttta ggcgctctac
     4981 cgcttcctct aagatttcgt aatagagctc ttgattcaaa taatccgatt ccttgatctc
     5041 atgatagatc aggtaattga ttttttcttg aagcgcagta tcaggaatca ctgtttcgaa
     5101 accaagattt ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag
     5161 aatcgctact ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat
     5221 atgcaagata ggaatatccg ttgccgcttg taactcttca aaaaagtagt gtgcggtgtt
     5281 acaagtcaac acaatgaagt ttggtcgcaa taaattttgt ttctcaatat catccagcag
     5341 aaacggcatt ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt
     5401 tgcatgattg aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg
     5461 gttgatcaag cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa
     5521 aaaattctcc atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata
     5581 gttatggaac atatatttca tcaacaacca gcgcttgatt gatcggtctt tttcataaaa
     5641 tacagtagta ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt
     5701 ttcccgagca tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc
     5761 tgattgattc gttccatagg tcgtttcaac aaagggtttg ttgaacactc gatcttctgt
     5821 gacaaaacga gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct
     5881 caaattgatt tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt
     5941 ggcaaagcct gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc
     6001 gttataatct ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa
     6061 tagtggatga ccaaggcaca tcatccgaac ttgatgatct tcatctacat acgcatttag
     6121 cacacgcata ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata
     6181 accggcttcg tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa
     6241 agcttttttt cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa
     6301 agcaactggg aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc
     6361 tctgacgatc aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct
     6421 tactttattg atcagccttt caaataaaga ataatcaata tatggacaga taaaatattc
     6481 tgacagctct tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa
     6541 aagatatttt ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat
     6601 aaatacaggg tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg
     6661 ggtaggggct aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa
     6721 agaacgtgcc ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc
     6781 attttcaata ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc
     6841 aactacacaa ggaatatcat accataaaga aaaaagcaat caaaggaaag tcctccgatt
     6901 gctcaattct tttttgaacg gattgaactt ataggaagtt tttatctata ccaatcagtg
     6961 tagaaagaac taccatgtaa tgattctaga acactgatat taataaaaca agcaaaaatt
     7021 cattttttta tgttttttta actatctcag ctatctatcc tctttatacc attatttttt
     7081 tcaaatagtg acctgtataa ctttctttta cgttaatgat ttcttctggt gttccagtcg
     7141 caaggatcgt tcctccacct tctccacctt ctggtcctaa atcaatgata taatctgctg
     7201 ttttgattac atctagattg tgttcaatca ctaaaactgt gttaccagca tctactaatc
     7261 tttgtaatac atgcaacaag cgggcgatgt catcgctatg aagtcctgtc gttggttcat
     7321 ctagtatata gaaattcttt ccattagaga ttttgtgcaa ttcactggca agtttcatcc
     7381 gttgtgcctc accaccggac aatgtcgttg ctggttgccc catcgtcaca taacctaagc
     7441 caacatcaac aatcgtttgc agtttgcgat gaatctttgg aatgtgcttg aagaattcta
     7501 cagcatcctc tactgtcatt tccaaaatat cagcaatgct ttttcctttg taatgcactt
     7561 ctaatgtttc agaattatat cgtttaccat gacatacttc acaaggaaca tagacatcag
     7621 gcaaaaagtg catttcgatc ttaataattc catccccgcg acaagcttca caacgaccgc
     7681 cttttacgtt gaaactaaaa cgccctttct tataaccccg catttttgcc tcgttcgttt
     7741 gagcaaataa cccacggata tcatcaaata cacttgtata agtcgctgga ttacttctcg
     7801 gcgtccggcc gattgggctt tgatcgatat cgatgatctt ttcgatactt tcgtagccgg
     7861 aaattgtctt gaatttacct ggcttagcag aattcttatt taatttttgc gctaacgatt
     7921 tctttaagat actattgacc aatgtactct ttccagaacc agacacacct gtgactgcta
     7981 caaattctcc taatggaaat tcaacactaa tatttttcag attgttttct gaagcaccag
     8041 tgatcttgat tgctttccca ttacctttac ggcgttcttt tggtacaggg attgattttt
     8101 tccctgaaag atatttccct gtcaaagaat tttcatcatt agctacttct tcaggagtgc
     8161 ctgcagccac gatttgtccg ccttgttctc cggccccagg tccgacgtca atcaaataat
     8221 ctgctgccca catcgtatct tcatcatgtt cgactacaac gagggtgttg ccaagatcac
     8281 gcattttctt taatgagcca atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg
     8341 gttcatctaa aatatacaaa actccagaaa gattcgatcc aatttgtgta gccaatctga
     8401 ttcgctgcgc ttctccccca gataatgttc cggaagcgcg actcaaagtt agatagtcta
     8461 gtcccacatt ttccaagaat gaaagtctgt catttacttc ttttaaaatc ggtttggcaa
     8521 ttactttctc ttgttcagat aacgttaagt ttgagaagaa atctactgct ttttgaatag
     8581 acaactcatt tgtttgcccg atattcgttc cattgatttg aacagataga gcttggggat
     8641 tcaatcgata acctttacat gtctgacaag ttaattctgt catataaaga cgcatttgct
     8701 cacgcgtgaa atcactgttc gtatcatgat aacgacgctc gatattcttc aatacccctt
     8761 cgaaagtcgt ttctacatcg cgaacgccac caaaatcatt ttcataatgg aaatggaaaa
     8821 gatcatctgt cccattcaag atgatctcct gatgctcttc cggtaattct tcgaatggtg
     8881 tatctaaatc gataccgaaa ctttcacaag cctgcgctaa catttgagga taatactgag
     8941 agctgattgg attccatgga acgatagcac cttgactaag tgtcttcgtt ctatctggaa
     9001 tcactaaatc ttgatcgact tctaatttga cacctaaacc atcacagtct ggacaagctc
     9061 caaaaggcgc attgaatgag aacagtcttg gttctagttc gccaacagta aatccgcaat
     9121 aaggacaggc atagtgttcg ctaaacaaca tttcttcttc accgatcacg tcaaccagag
     9181 catagccgtc tgctaatcgc agggccgcct ctaaagaatc aaataggcga gaacgaatcc
     9241 cttctttcac aatgatccgg tcgatgacga tcgcgatatc atgttttttg tttttctcca
     9301 gctcaggcac ttcacttaaa tcatagatct caccatcgac tcgaacgcgg acgtatccct
     9361 ctttttggat tcgttcgaat atttttttat gttgtccttt tttctttacc acgatcggtg
     9421 ccaacacttg gatctttgtg cgctcaggca aagccattac ttgatctgcc atttgatcta
     9481 ctgattgact ggtaatctcg atatgatcgt tgggacaaat cggatgtcct acacgtgcaa
     9541 acaacaagcg taaatagtca ttgatttccg tcacagtacc tactgtcgac cgaggatttt
     9601 tacttgttgt tttctgatca atagaaatcg ccggactcaa tccgtcgatg ctatctacgt
     9661 ccggcttatc catttgtcct aaaaactgac gagcataagc agaaaggctc tctacgtatc
     9721 tccgctgccc ttcagcgtac agcgtatcaa atgctaggga actttttcca gaaccggaca
     9781 aacctgtaac gaccacgaat ttatctcgtg gaatcgttac atctatattt ttcaagttat
     9841 gggcacgtgc accatgaatc gtaattttat cgttagccat gttgtcctcc tactttgaag
     9901 cttttaattc taaaatcata tctcgcacat tcgcggctgt ttcaaaatcc aaagcttttg
     9961 ccgcatcttt catttctttt tctaatttca tcaataacgt gtctttttct tccttcgtca
    10021 tttcttcgta cgagacttgt accgcttctt ttgtatcatc ttccgattct ttcgtaatag
    10081 aaatcaaatc tcggatttct ttaatgatcg tttttggaac gatcccatgt tcttcattgt
    10141 acttctgctg aatcgtacga cgtcttgacg tttcatccat cgctagacgc atagaatctg
    10201 ttactttgtc tgcgtacatg attactttac cttcttcatt ccgagcagct cgtccaatcg
    10261 tttggactaa ggaacgttca ctacgtaaaa agccttcttt atccgcatcc aagatagcaa
    10321 caagggaaac ttcaggtacg tctaatcctt cacgaagcaa attgattcca actaacacat
    10381 caaattcacc taatcgaaga tctcgaatga tttctgttcg ttccagcgtt ttgatatcac
    10441 tatgcaaata tttgactttg atccctaatt ctttgaaata atctgtgagg tcttctgcca
    10501 tttttttcgt caatgtcgtt acaaacaccc gctggtcttt ttcgactcgt tcatttattt
    10561 cgcctactag atcatcgatt tgtcccatga ttggacgaac ttcgatcact gggtcaagca
    10621 gccctgtcgg acggatgatt tgttggataa cggtatctgt ttgttcatgt tcatatggtc
    10681 ctggcgtcgc agagacataa atgatctgat tgacatgttt ttcaaactct tctaaccgaa
    10741 gtggccgatt gtctaaagca gatggcaaac ggaaaccgta atctactagc atctgttttc
    10801 tcgctcggtc accattatac attcctctga tttgaggcat ggttacgtgc gattcgtccg
    10861 ccacgatcaa aaaatcttca ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt
    10921 tacgtccgtc catatgccga gaataatttt caattcccga cgtgtagccc atttctaaca
    10981 tcatttcaat atcgtaattg gttcgctgtt ctaaacgttg agcttccaga agtttgtttt
    11041 catttcgcaa gacagttaaa cgctgttcta attcctcttt gatattcgct acggcatgct
    11101 ccatatgttc atcattcgtc acaaagtgag tcgccgggaa gattgctacg tgttccgttt
    11161 ctcctaaaac ttcacctgtc agtgcattga cttctcggat acgttcgacc tcatctccaa
    11221 aaaactccac acgtaaggca cgttcatctc ttgaagccgg aaaaatctcc acgacatcac
    11281 cgcgcactcg aaaacgaccg cgctgaaaat ctatatcatt tctttcgaat tggatactga
    11341 ctaagtcacg aatcaactga ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt
    11401 gtttttggta ctcaaaaggc gagcctaatc caaaaataca tgaaacggat gcaatgacga
    11461 tcacatcatt tctttctaat aatgaactag tagccgaatg tcgtaatttg tcgatctcat
    11521 catttacact tgaatctttt tcgatatacg tatcgcttga aggcacataa gcttcaggct
    11581 gataataatc ataataagaa acaaaatatt caacagcatt gttagggaaa aattccttga
    11641 attccccgta cagctgacca gccaatgttt tgttatgggc aataatcagt gttggtttat
    11701 tcactttctc aataagattg gaaatcgtat atgttttccc tgttccagtc gcacctaata
    11761 agatttgtgc ttttttcccg ccgactacgc catccactaa ttggttaatc gcttcaggct
    11821 gatcaccagc cggttggtat ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa
    11881 tcattcttcg tcctcctttt cattcattcc ttttcatttt acgaccaata aaaagaaaag
    11941 gaaagcaaag gcattcctta ctaaaaaatt ataccacacc gaacatactt tcgccacttt
    12001 tttatcttat tatacatgtt atgttttata acataaaatg ttagaaactc gcattttttt
    12061 cattttttcg agcttacagt cttgtctgat tcagcacttt tcgcctataa taataaaata
    12121 ttggaaattt aggaggttga ttatgaaaag aaagcattta tttcactcat ttgttttctt
    12181 aatgacagga cttgtgacac ttgcgtttgg tcatgttgca caagctgaca acactacaac
    12241 ggcagagtca agcacaagcg agacaatgca aaaaatagag cccaaaaaag acgtatacgt
    12301 aattgccagt gactctgctt ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga
    12361 aggcatcgat gtaaaattga tggaaaacat cgccaaactg caaggattta acatccgaat
    12421 ggactaccgt ggtttctctg ctgctttgca agctttggaa tcaggacaag cagacggaat
    12481 gattgccgga atgactgtaa cagatgaacg aaagaaaagc tatgattttt ctgattctta
    12541 ctttgatagc gggatccaat tagctgtcaa aaaaggagat acttcgatca agtcttatga
    12601 agatttgaaa ggaaaaacag tcggagcaaa agttggtacc gaaagtgctg aatttttaag
    12661 cgaccatgaa tctgaatatg gctattctat caaatatttt gatgctgctg atcaattgta
    12721 cgacgggtta aaagttggcg caatcgatgc aatgatggat gattatccgg taatcggtta
    12781 tgcgatcaaa caaggacaag aattggaaac accgatcaaa aaagaatctg gcggaaaata
    12841 tgcatttgcc gttaaaaaag gacaaaatcc tgagcttctt aatatgttca atgaaggatt
    12901 gaaagaatta aaaagaacag gcgaatacga taagattatt aaagaatatg tagcggatgg
    12961 ttcagaaact tccagtgagt cttccactgc tgatgaatca acatttcttg gacttatcca
    13021 aaacaactat caagcattgt tgaaaggact ttggagaaca attctcctaa ctttgatctc
    13081 ctttgctttg gctatagtaa tcggtgtgat ttttggatta ttcagtgtag caccaatacg
    13141 cgctcttcgc acaattgcta gtatttatgt cgatatcatc cgaggaattc caatgatggt
    13201 tcttgcgttc tttattttct ttgggttgcc tggtatcgtt ggatttacga ttcctgactt
    13261 ccttgctggt gttatcacct tgactcttaa cgcaagtgct tatatcgcag agattgttcg
    13321 aggcgggatc aacgccgttc ctgtcggaca aatggaagct tccagaagtt taggtctttc
    13381 ttataatcgg acaatgcaaa aaatcatttt gccgcaagcg atacgcatca tgatcccatc
    13441 attcgtcaat cagttcgtta tctcattaaa agacacaacg atcatttccg caatcggtgt
    13501 agtcgaattg ctgcaaactg gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt
    13561 ttacttgatc attgcgatca tgtacttgat tttgatcaca gcattgacaa aattagcaaa
    13621 agttttagaa aagaaggtga agtaagatgg cagaaaaaat cttagttgag catttagtca
    13681 aaaaatacgg cgataacaca gtattaaacg acatcaatgt atccatcaac gaaggagatg
    13741 tcgtctgtat cattggccct tcaggttcag gaaaaagtac attcttgcgt tgcttgaatc
    13801 gtttagaaga accttctgac ggtgaaatta ttattgacgg cgcccacttg atggataaaa
    13861 gcacagatat caacttggtg agacaacata tcggaatggt attccaacac tttaacctat
    13921 tccctcatct ttctattttg gaaaacatca ttttagcacc gatggacctg aagaaagaat
    13981 ctagagaaga agcagagaaa aaagcaatca aattactgca gacagttggt ctagaagata
    14041 aaaaagatat gtatcctgaa atgctttctg gtggacaaaa acagcgtgtg gctattgctc
    14101 gtgctctagc gatgaaccct gacatcatgc tttttgatga gcctacttct gcactcgatc
    14161 ctgaaatggt cggtgatgtc ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga
    14221 tggttatcgt tacgcacgaa atggggttcg ctaaagaagt ggccaatcgc gtcatgttca
    14281 ttgacgacgg aaacttttta gaagatggaa cgcctcaaca aatctttgaa aatccgcaaa
    14341 atgaacggac aaaagatttc ttgaataaag tattaaatat ttaaaataaa ttatatatgt
    14401 ctttttgact atctttttat atcttcttaa atgtccgcaa agccttattt tataggctct
    14461 acgggcattt tgcttttgtg gtaaacctca catatctagg tctatcttct tatattttca
    14521 ctatcaagcg tggttaaaat cgtggtaaat acttctatgc aattagacgc tgaacctctg
    14581 attttgcggt atctatggac gcatgagcat accagttcat tgtgatactg atatttgaat
    14641 gccccatgat atactgtaaa tcttttgggt tcatgttctt gcttgccagt cttgtgcaga
    14701 atgtatggcg tagcgtatgc ggtgtgatat gtggcaatgg attgtccttg tggtgcttgt
    14761 tgtatttctt taccatacgg ataaataagg cgttataatc aatcgcaact ttgggcttgc
    14821 ccttatgatt gacaaatagg aagttgcccc gtccgtctat cacaaatggt tctgcctttg
    14881 gacgtttttt cataacctgt tgaaatgcct gtatcgtttc tttgcttaat ggtacttgcc
    14941 ttattccgct ttttgtttta ggcgtttcaa tataatagcc ctgttccttg ctctttagta
    15001 actggtggtc gataatcaca acttcattct tgaaatcaat gtcggctact gtcagtccgc
    15061 acagttccga aatacgaagt cctgtcttta acagtatcag cacatcatca taatgcttgt
    15121 gatacacatt gtccgtcttg atgaatgaga gtaaggcttg ttcctgttcc tctgtcaatg
    15181 cgactttctt tttggtatca ttttctagga cttcactcaa cttgaaatca aagggatttt
    15241 tcctcacata atcatcttga atggctatgt agaatgacgc ttttaacgag cgtttatggt
    15301 tgttaatggt attgtaggaa aagcctttgt ctttcatgcg taacgcccat tccttagcgt
    15361 cagagggttt gattgtatca atgctcctag cacctaactt gtcctctttc aataaccgca
    15421 tgagttgttc ccgttgtttc tgtgtgcttt tcttcacatt tgccctctgt gcgttctgtt
    15481 tggcataaag ttggcaaagc gtcattttct tgccagtgct gtcgataccg tcctcaatat
    15541 cccgtcttat ctgctgttcc agttctcgaa gtgagggttt ttcccgtttt ccctttggtg
    15601 tcgggtctgt gggtgtcaat ctccaagcat acacatattt tgtgtttcca aatgcgtcta
    15661 catatttata taagtatttt ctgtctgttc gttggctctc tccagtatgc aggatacgac
    15721 ctttgttatc ccgtcttttt tctttcatgg tgtctgctcc tttccttgtt ggaaagagcc
    15781 ttgatatgac ttgtgttcat cataacacat acaaggctca tttgcattag atagcgtcca
    15841 atttatcaat gacctgttca aactgtcggc gtttaatctg tatgcggttg ccattcataa
    15901 tgagccagcc agcgtccttg ttttcctctg ccaatcgtct taacttgttt tctccgatac
    15961 ggaaatactt tgacgcttct tcaatggtaa gggtgtattt ttcccatacg ggaatatcct
    16021 gtgcattact cataaaacac cccctcactt tcgattacgt ctgctatgtc cgtacacatc
    16081 tgttccacaa gcatttcctg cttttcagag aggtgggtac attcatcaat cattttcatg
    16141 tagtttgagc ctgtcagcct gtcgatttcc tctatcatca cgattgacgg catgacctgt
    16201 ctttccagcc attgcagagt gcgttccata ttgacaggta cggggttcat ttcaaaaggt
    16261 aaactgtcat gggaaatgaa tatctcccac agcggatagt cgggaaattg cacaaaatca
    16321 gtaataaggt aaaacaccag cccatgaggg ttatatgtca ataacaattc ctcaactgcc
    16381 tgtactgctt ttgtaccacg cagacgaatt tcaaaacgat tgataatatc cgtatgtttc
    16441 tttttggttg cctgttcctt ttgtttttca tacaggcaga agtattttgt gcttgccttt
    16501 gaaccgatat aaagggtgct tgctaaatta cggttttttc cgcttagttt tccaccctgc
    16561 acattttcat aatttttaca gcgacaatcc gcaccgccat ttctgtattt ttcagacaaa
    16621 acgggaatat ccaacagacc acacatatca ttgattgcca agtcaaaacg tcggataaca
    16681 ccgtcacagt caagacagcg gtttaaaaat gaataccaat ctctgttctg tgcctgcaag
    16741 acatattcca tgttgcgtga gcctgcccct tttaattcca agaatacgcc catgtgttcg
    16801 tcatcagaag ccatgacctt gatttctccg taggcgtaat gctctttata gccataatac
    16861 ccatagtcat aatggataaa ataatctgct ttcatgccaa gcacctttcg gataatttcc
    16921 aatgcgtcag tcgttggata acggacagac agataatcaa cgagcagata aaaaggctta
    16981 tcgcagatat aataattcag acaacgcagg ataatatttt gtgtttcctt gctggcgttg
    17041 gcttttccgt tctcaaagcg gttgatacta tatcttgata catgggtaag acttgcaagt
    17101 tctccttgtg aaagaccgct tgcaagacgt ttgttcttca tttcaagtgc aaattctaaa
    17161 ttgttcattg tattcctcca atcccacaat agccttgaaa tacctgtaaa cctctattca
    17221 aaaagtgtga tatgcactat cttcttaaaa cttctcatta ctactcacaa ctttgggatt
    17281 gtatggtttt tgacttgtct tttgtgaaat agtgcccccc tgttagataa cgggggcaca
    17341 gacaaggctc gcaggctcgc cagccgacaa agcatttctt ccccttgaca caaggtgtca
    17401 tggcggtaag cctgagaggc ttccgccacc gtgtcagaaa tactttgtca gcaagttctg
    17461 ttttgccttt tctaacaagt tccatttcaa ccgatattca tatctacgga tttaatcaga
    17521 taggtatttc tttttgaatg tttttatatt tcattctatt gtgcttattc gattacaaga
    17581 acatagtaat tcttatatgc ttatttgtca atatcttttt ttgcctttac aaatcatatt
    17641 aagcatgata gacttataat agcgaagata taacgagata tggagggtta tgcaatgaaa
    17701 ataagccatt taggaaataa tatacagacc ataagaaaat ttaggggaat gaaacaacag
    17761 gaacttgcgg acaaaatcgg tatcaatatg cagagccttt ccaagattga aagagggttg
    17821 aactatcctg cctatgaaac gctggaaaag ataatggaag tgctggacgt tacgcccaat
    17881 gaattgttgt cgggagaatg gaagtatgtc aatcaatctg aaaaggaagt ctgtcagttt
    17941 ttaagagccg aagaacgtct aaatgcagaa ctgaaacatg gacactatga taacttcttt
    18001 gacagcgagg aagaatggct ggaatatgag ttagaaaagt tacgggaata tattacagac
    18061 tatatcaacg ggaaaagcat tgaagcgtcc gacctttacc caatcaaaga atttatccag
    18121 catttgaagt ttcaaaaatt attagaccgc tatgatgatt tatacagtat ggatatgttt
    18181 ggggaaagca tagagggaca taagtataag acaccttatc aagtcgtgaa aatgataaac
    18241 ccgaactcaa aagaagatat ggaactgtta cgagaagtat taaggaataa caattttgat
    18301 gatgaggacg agtaactttt ttcctcttgc gagaagatac agcaaacaaa aagcccgatc
    18361 aagagtgcaa agcaccacca atggtggcaa agctcttgac agggcttttt cttttctgtt
    18421 gtgggcaatc aagaggaaga aagggataaa atctgatttg tggtaaatca ggcaaaggca
    18481 acgcaggtat ctcaatttga tttggtactt gaaaagaacg ggagtaattg gaagattata
    18541 gaataacaaa tattggtaca ttattacagc tattttgtaa tcacgtactc tctttgataa
    18601 aaaattggag attcctttac aaatatgctc ttacgtgcta ttatttaagt atctatttaa
    18661 aaggagttaa taaatatgcg gcaaggtatt cttaaataaa ctgtcaattt gatagtggga
    18721 acaaataatt ggatgtcctt ttttaggagg gcttagtttt ttgtacccag tttaagaata
    18781 cctttatcat gtgattctaa agtatccgga gaatatctgt atgctttgta tgcctatggt
    18841 tatgcataaa aatcccagtg ataaaagtat ttatcactgg gatttttatg cccttttggg
    18901 tttttgaatg gaggaaaatc acatgaaaat tattaatatt ggagttttag ctcatgttga
    18961 tgcaggaaaa actaccttaa cagaaagctt attatataac agtggagcga ttacagaatt
    19021 aggaagcgtg gacaaaggta caacgaggac ggataatacg cttttagaac gtcagagagg
    19081 aattacaatt cagacaggaa taacctcttt tcagtgggaa aatacgaagg tgaacatcat
    19141 agacacgcca ggacatatgg atttcttagc agaagtatat cgttcattat cagttttaga
    19201 tggggcaatt ctactgattt ctgcaaaaga tggcgtacaa gcacaaactc gtatattatt
    19261 tcatgcactt aggaaaatgg ggattcccac aatctttttt atcaataaga ttgaccaaaa
    19321 tggaattgat ttatcaacgg tttatcagga tattaaagag aaactttctg ccgaaattgt
    19381 aatcaaacag aaggtagaac tgtatcctaa tatgtgtgtg acgaacttta ccgaatctga
    19441 acaatgggat acggtaatag agggaaacga tgacctttta gagaaatata tgtccggtaa
    19501 atcattagaa gcattggaac tcgaacaaga ggaaagcata agatttcaga attgttctct
    19561 gttccctctt tatcatggaa gtgcaaaaag taatataggg attgataacc ttatagaagt
    19621 tattactaat aaattttatt catcaacaca tcgaggtccg tctgaacttt gcggaaatgt
    19681 tttcaaaatt gaatatacaa aaaaaagaca acgtcttgca tatatacgcc tttatagtgg
    19741 agtactacat ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga
    19801 aatgtatact tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat
    19861 tgttattttg caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt
    19921 gccacagaga aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag
    19981 taaacctgaa cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc
    20041 gcttctacga tattacgtgg attctacgac acatgaaatt atactttctt tcttagggaa
    20101 agtacaaatg gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg agatagaact
    20161 aaaagagcct acagtcattt atatggagag accgttaaaa aatgcagaat ataccattca
    20221 catcgaagtg ccgccaaatc ctttctgggc ttccattggt ttatctgtat caccgcttcc
    20281 gttgggaagt ggaatgcagt atgagagctc ggtttctctt ggatacttaa atcaatcgtt
    20341 tcaaaatgca gttatggagg ggatacgcta tggctgtgaa caaggattgt atggttggaa
    20401 tgtgacggac tgtaaaatct gttttaagta tggcttatac tatagccctg ttagtacccc
    20461 agcagatttt cggatgcttg ctcctattgt attggaacaa gtcttaaaaa aagctggaac
    20521 agaattgtta gagccatatc ttagttttaa aatttatgcg ccacaggaat atctttcacg
    20581 agcatacaac gatgctccta aatattgtgc gaacatcgta gacactcaat tgaaaaataa
    20641 tgaggtcatt cttagtggag aaatccctgc tcggtgtatt caagaatatc gtagtgattt
    20701 aactttcttt acaaatggac gtagtgtttg tttaacagag ttaaaagggt accatgttac
    20761 taccggtgaa cctgtttgcc agccccgtcg tccaaatagt cggatagata aagtacgata
    20821 tatgttcaat aaaataactt agtgtatttt atgttgttat ataaatatgg tttcttgtta
    20881 aataagatga aatattt
//
LOCUS       DAJSKS010000039        20835 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJSKS010000039 DAJSKS010000000
VERSION     DAJSKS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.040"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(33..1076)
                     /locus_tag="ON396_001217"
     CDS             complement(33..1076)
                     /locus_tag="ON396_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3113315.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
     gene            complement(1237..1920)
                     /locus_tag="ON396_001218"
     CDS             complement(1237..1920)
                     /locus_tag="ON396_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HCR3113316.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            2115..3044
                     /locus_tag="ON396_001219"
     CDS             2115..3044
                     /locus_tag="ON396_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR3113317.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            3105..3896
                     /gene="phnC"
                     /locus_tag="ON396_001220"
     CDS             3105..3896
                     /gene="phnC"
                     /locus_tag="ON396_001220"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR3113318.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            3868..4647
                     /gene="phnE"
                     /locus_tag="ON396_001221"
     CDS             3868..4647
                     /gene="phnE"
                     /locus_tag="ON396_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCR3113319.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            4644..5426
                     /gene="phnE"
                     /locus_tag="ON396_001222"
     CDS             4644..5426
                     /gene="phnE"
                     /locus_tag="ON396_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCR3113320.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            5429..6232
                     /locus_tag="ON396_001223"
     CDS             5429..6232
                     /locus_tag="ON396_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR3113321.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            6316..7863
                     /locus_tag="ON396_001224"
     CDS             6316..7863
                     /locus_tag="ON396_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCR3113322.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(8160..8357)
                     /locus_tag="ON396_001225"
     CDS             complement(8160..8357)
                     /locus_tag="ON396_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113323.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(8433..8633)
                     /locus_tag="ON396_001226"
     CDS             complement(8433..8633)
                     /locus_tag="ON396_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3113324.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            complement(8753..9940)
                     /locus_tag="ON396_001227"
                     /pseudo
     CDS             complement(8753..9940)
                     /locus_tag="ON396_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9985..11111)
                     /locus_tag="ON396_001228"
     CDS             complement(join(9985..10883,10883..11111))
                     /locus_tag="ON396_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR3113325.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(11515..12297)
                     /locus_tag="ON396_001229"
     CDS             complement(11515..12297)
                     /locus_tag="ON396_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113326.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(12362..13198)
                     /locus_tag="ON396_001230"
     CDS             complement(12362..13198)
                     /locus_tag="ON396_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3113327.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(13216..14625)
                     /locus_tag="ON396_001231"
     CDS             complement(13216..14625)
                     /locus_tag="ON396_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3113328.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(14639..16462)
                     /locus_tag="ON396_001232"
     CDS             complement(14639..16462)
                     /locus_tag="ON396_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3113329.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(17305..17631)
                     /locus_tag="ON396_001233"
     CDS             complement(17305..17631)
                     /locus_tag="ON396_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113330.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(17677..18039)
                     /locus_tag="ON396_001234"
     CDS             complement(17677..18039)
                     /locus_tag="ON396_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCR3113331.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(18076..18290)
                     /locus_tag="ON396_001235"
                     /pseudo
     CDS             complement(18076..18290)
                     /locus_tag="ON396_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            18418..18753
                     /locus_tag="ON396_001236"
     CDS             18418..18753
                     /locus_tag="ON396_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3113332.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(18829..20826)
                     /locus_tag="ON396_001237"
     CDS             complement(18829..20826)
                     /locus_tag="ON396_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113333.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
BASE COUNT         6231 a         4207 c         3677 g         6720 t
ORIGIN      
        1 actgtagaca tcatccataa aatggacttt gtctacagtc tgaaatcacc actgatgatt
       61 ttctcttttg cgtctgaaac tgcctgatag gcttcctctg ataattgtcc ttttgtcaga
      121 tctacccctt cttctttcaa accaaaggtc aaatgcttgc cgccaggaaa tttatcgtta
      181 tgagaagttt ctgcaaattc tttgacttct gcaccgacat ttttctttgt agaagtcaac
      241 gtgaaattcc ctgtctcgcc gttctccatt ttataatccc cttcatccgc ttgatcgcga
      301 tctacaccaa tcacccatac ttttctgcta tagtcgtttt cattgcgggc ttttgcttct
      361 tggaacaccc cagctccagt acttcctgcc gcctgataaa gaatatcgac tccctctttg
      421 tacatagaag cagcaatagc ttttccttta tctagcgaat cataggatgc agcatattgt
      481 acgttaacag taatttcctt gttcagtgct gtggcccctg ccttaacgcc agcttcaaat
      541 ccgctttgga aacgttggat cacgggccct tccattccac caataaagcc tacttgatca
      601 ctttttgtcg tatatgcggc agctagtcct gctaaatacg cactttcttg atctttaaag
      661 gttgctgatg agatattctt tttcccagta atctcaccat ctaccaaggc aaatttcaac
      721 tttggattat ctttagcagc attttccagt tcctctgctt gcaagtagct caagctaata
      781 atggtggtaa agccgtttct gattgcctga tcgatattgg tactgttatc ctctggattg
      841 ttcgtttcta tatacgtaaa atcttttttt gctgtcagct ttttctcttt tccccaagcc
      901 tctaatcctt cccagccagc ttgattgaag gagcggtcat caaccccgcc aaatccaaga
      961 accaaagcga ttttactttt ctctttttca ctttgcttct ccccctgaga ctgactacag
     1021 ccgactaacg ttactaatga caccaaaaac aacaatgaaa caagtatttt cttcaaaatt
     1081 tttctcccct ttttatgtgt cttgacacat tgaactttta aaagtatagc ataccaatat
     1141 atagaacact actcttcaac gaagattttc cgtttcccaa tttctccact atcaaaaaac
     1201 cagcaatcac ttctgactgc tgttttttta tagctgttat tctgctttta ctggactact
     1261 tatattctca gctaagtcct ctaaagcttc gatatcatga atcaagtaat aattcttttt
     1321 cttttcgata atattctcct cacgaaaatt cgtgattgtt cgtaaaagat gtcgataact
     1381 gacatttaag attgaggcgc aggtggttaa ccgaaatgag aaacgttcgt tttcgtgatg
     1441 ggtcaaaata aactttgcta aacgagtttc aacaggttct gtcagtgagt tcgccaaatt
     1501 tatcccggaa tttaaacgat aacttagtaa ctggcaaata ttctgcaaaa agcgaacatc
     1561 tttttgaagc tgtgttcgat attgatttag cggaatgctg atacagatgc acttagtcac
     1621 tgctttaacg ctgcttttcg gcgataattc ccacaatgaa gaggcttcgc cgagtggtgt
     1681 agcgggcttt gcgtaatcga tgcaaatatt ttgtgtatca gaagaatacg agtacaccat
     1741 taccgtccca tccactaaaa aatacaaata atctgaggtt tttcccgttt gaatgaggtg
     1801 ctcttccttt tcaaaaaaaa gtaaagaagc cattgccaat atatctgaat ccatataatc
     1861 ctgcaggttg tgcttctcaa cgtaagcagt taattgcttc gtatcatgga tttttttcat
     1921 gaaatcgtca cctctaagaa gtttttcttt attatagctt agattgtttt tcggctcaat
     1981 acgaataatt atttataatt gtttgtaaat tgtatgtaaa agttgtgtaa aataggacgt
     2041 gcgtcacttc attgattttg attccctgct atgatttaga cgtattagaa attatatcca
     2101 acaggaggag aaaaatgcag ttcaaacagt tagcaaaagg gttcatcgga gtcatgttat
     2161 tgaccacttt ggcggcctgc ggttctggga atacaagtag cagctcagaa aaagagagca
     2221 aagaaaaatt agttgttcaa ttcgttccaa caaacaatga tggtagtatg gaggcaaaag
     2281 caaaaccatt tgctaagtat ttatcgaaaa agttagatcg tgaggtagaa gtgacgttag
     2341 ctactgatta ttcaacgatc gtcgaagcga tgtcttctgg tcaagtcgat gtcggaatta
     2401 tgccgcctgc agcctatgtt caagcaaagg atgctggagc tgcagatgca atcctgactt
     2461 ctcagctcgg cgattatgat caagaaactg gcttgccatt aaaggacaaa cttacgaaca
     2521 cctttaaagg agaagtattg gttcgtgccg atagcggatt gaataagtta actgatttaa
     2581 aaggaaaaaa tatcgcaacc ttaagtccta actctgctag cggctacatc tatcctgtcg
     2641 ctgaattgaa agatgctggg attgatccta ctgcagatac aaagatgacc actgtgaatg
     2701 atattccgag tgagattacc gcagtgttga acaagcaaat ggatgcggcg tttgtttttg
     2761 aaggcgcgcg aaatgttttt gcttcgaatt tcccagataa tgatttattt aaggatttaa
     2821 aagtcctgta cctgacaaaa ggcgatattc cgaatgacgc aatcgctgta cagcctgaca
     2881 tgtcaaaaga tttaaaagcg aaaattaaag agaccttctt aaatatgaag gatgataaag
     2941 atggcaaaga agcaatggct ttgtgggggc atcagggcta tgaagaagct gctgattctg
     3001 cctatgacac gatcagagac tacacgaaaa aagcagcaga ataaaacatc agtaagtgtc
     3061 agcaagcttt tttttgctta cacttaaaaa aatggagaag gataatgatt atttttgaca
     3121 atgtttcgaa ggtctatccc aatggcgttg ttggcttgaa ggatattgac ttaacaattg
     3181 aagatggaga atttgtttcc atcatagggt tgagtggtgc tggaaagagc actctgctgc
     3241 gcagtatcaa tcgcttggca gaaaccacca aaggagatat tcgcattaat ggaacctcta
     3301 ttacaaaagc gaataaaaaa gagctgcgac agattcgccg ccagattggt ctaatctcgc
     3361 aaagcttcaa tctggtaaag cgtagtacgg ttcagaaaaa tgtgctatct ggacgtttgg
     3421 gctattactc cacttggaaa agtattttag gtctttttac gaaggaagac taccagcaga
     3481 caaaggaagc actggaaaaa gttggtttgt ctgataaatt gcagacacgc agtgatgagc
     3541 tcagtggtgg gcaacaacag cgagtatcca ttgcgcgagc actcgtgcag caagccccca
     3601 ttattttagc ggatgaaccc gttgcatcat tagatccaat cacgactcaa aaggttatga
     3661 tggacctaaa aaatatcaat caaacgatgg ataaaacagt cattgtcaat ctgcattcag
     3721 tccctctggc tcgtgagttt tctacgcgtg tgctcgcgtt aaaagctgct caggttgtct
     3781 ttgatggtcg tccagacgac ttaaccgatg aacgcttaga gcaaatttat ggcaatgcaa
     3841 tctttgaaga aaaggcaggc gaaatcgatg agcttgaaag aaaccgttca ctttaaatgg
     3901 tataaacacc tgcttattct tctgttctta attgtttgtt ttcaaggaag tgcctggatc
     3961 acgtcagctg atttttctca ggtttttcaa aactccaatc aaatgactgt ctttttatca
     4021 cgcttcattc atccagactt tagctattta cctaaattag tcgagcctat ggtgaaaaca
     4081 cttcaaatgt ctttgcttgg aacggtcatt ggcttaattt tagcgatccc tgttagtttt
     4141 ttagcgacca ctgtagtgac agaaaaccgt cttctttcta ccatctttcg ttttctttta
     4201 ggtttagtac ggaccatccc taccctgctt ttagccgcgc tttttgttgc tgtgtttgga
     4261 attggtgaag cgactggcgt gttgactatc gctgtcttta cttttggtat ggtgtcacaa
     4321 ttaatgtttc aagcaattga aaccattgac tttggcccga ttgaagccgc tgcggctgtt
     4381 ggagcaaata aaatgcaaat cgctatttgg tcgatcgctc cacaagtttt gagtcagttt
     4441 gcgagctatg ctttttacgc gtttgaggtg aatgttcgtg cttctactgt ccttggatat
     4501 gtcggagctg gtggtattgg cgttattcta aattcctcac ttgcgctttt gaattatgaa
     4561 cgagtttcaa tcattattct aacgatccta gtaactgtcg cgatcgttga taaattaagt
     4621 gaacgtgtca ggaggtccct gttatgatgc ttcgtgaaat gaacagaaga acccttttat
     4681 cattagttgc tttagtgatt ctcgttattt tgtcggccgc tactctggat tactctggat
     4741 taagcacctt ctctccttcc atgggaatgg acgttttgag agggttaggt cagcctgatt
     4801 ggcagtttct atatgatggc agcggcgagg atttgattag tttgctatta ttaaccattg
     4861 gaatcgcctg tttagggaca atgatcgcta cggtattagc gattcccatt accttgatca
     4921 gtgcggttaa cctctggcaa gcacatccat gggtaacaaa aattggcaag cttatctgta
     4981 atgttttacg agcttttcct gaactagttt ttgcaattat ttttgtgaaa gtcgtcggac
     5041 ctggcccctt tgctggtgtt atggcgattg gcgtccatca gatcggaatg cttggcaaac
     5101 tttttacaga agaaatggaa gcgatggatg agcgacttgt tgaagaaatg catgcggtag
     5161 gagcaaattt ttggcaaacg attttctttg ttcgcatccc ctatttgatg ccgatttatt
     5221 gttctttagc attaaatcat tttgaaatag cggttcgtag tgcggctacc ttaggcttag
     5281 ttggcgctgg cggaatcggt gctccattga tattcgcgat tcaaactcgg tcttggagca
     5341 aagtgagtat tattttgatt ggcgtagtcg taaccgtgtt tattttagat caaataaccg
     5401 gaattataag aaagagattg agataagaat gaatatattg catatatcag atatacactt
     5461 tcgcagagag tatgaggctt gtgaagaagg ctataaggga atgctggcta cgatgcagaa
     5521 tccccttatc cctctggagc aatgcattca tcatctcctg cagcaaacac ccttggacct
     5581 tgtgattatc agcggagatt taaccgaaga tggcgaaata gacgattatc gatacttgaa
     5641 gacttggctg gaaaatgcgc taggagaaac gacgattctt gttactctag gcaatcacga
     5701 tatcaaagaa catttttggg taggctggtg cgatgaagcc gcctcttctg ccccgtacaa
     5761 ccagatagtc aaatatcctg agtttacagt gatttccttt gataattcaa gctatggata
     5821 cgcagatggt atagtggacg atcagcaatt tcaatggctg aaaaaagcct tagagcaaga
     5881 aaaagaccaa ccgattttct tggtcaccca tcatcatcta ctgtctcacc aaagcagtat
     5941 accaaaatgg cctggtaccg aacgtttctt gaagctaatc gcaccttacg atattcgctg
     6001 tatcctaaac ggtcataccc atcacacatt tactgatgaa attaatggga tcccctattt
     6061 tactgtatcg agtatgtcct ttgttggtga agatgaaggc gatggcttcg tacgctttga
     6121 agaacgtcat gggtataacc tttatcatct cgatcaagga aggattgttc gccagacaac
     6181 agaaaacttt attccaggca acattttaaa aacacttcaa atgaaggatt aaggcattat
     6241 aaatcaaaaa aagagaccga acagctgaat acgcttcggt ttttctattt tgagtcagct
     6301 aaaggagcac gcagaatgaa cattacgttt ttagagacaa gtgatatgca tggctatgtt
     6361 tcaccaacag actattccgg cagaaccgat ctggccttag gaacggctaa agtatcgtcg
     6421 aaactgaagg aattacgggc aaaagcagat ggaccggtaa ttacgatcga aaatggtgat
     6481 ttcatccaag ggtctccttt gagttattat cttgctaaag aatatggttc agccaaagag
     6541 cttacagact tgattaacca aatgagctat gatgttcaag tgttgggaaa tcatgagttt
     6601 aactatggtg tggattatct cgaggaagcg attaagaatt atcaagcgcc tatattagga
     6661 gccaatatgg tgaacaaaaa tgaccaacca tactttggaa acgcctataa gattatcgaa
     6721 aaggtgggtg taaaaattgc tatcttggga ttaacgacac aatacattcc tcactgggaa
     6781 aagccagaaa ccttagcagg aattacgttt aagagtatcg tcgaaacagc aaaagaatat
     6841 gttcctctat taaggaaaca agcagacttg gtaatcgtta gttaccacgg cggatttgaa
     6901 aaagacttgt taaccggtga ggacacagaa gcgctaactg gagagaatga aggctacgag
     6961 ctattgcagc aggttgaagg cattgacgcc cttttcaccg gtcatcagca ccggaaaatt
     7021 gctgaaacgg ttcatggagt tcccgttgtt caaccaggct atcgtggcga gttcatcggt
     7081 gaaattcgtc ttaccgtcga gaaaaaagag gggcaagtgg ttgttacaga tcaccaggtt
     7141 gctttacact cagttgaaaa atcagagcct gatccagaaa ttcttgcagc gatcgtaccg
     7201 gtagaagaaa aactagagaa atggttggat caacccttag gtaaagtgaa cggaaatatg
     7261 cgtattaatg atccgatgca ggctcgtctt gaggaacatc cctatgtaga gtttattaat
     7321 cgtgtacaat tatttgccag cggctcagag atttctggaa ctgcgttatt caataatgaa
     7381 ggaaaaggct ttggtgagac gatcacgatg cgggacgtca tcaccaacta tatttatccg
     7441 aatactctgg cagtgattca aataaatggt gcagatttga gagcggcgtt ggagcagacc
     7501 gcaaattatt tagctgtaga aaataaccaa atcgtattca atccagcctt tattcagcct
     7561 aagccccagt actacaatta tgacatgtat gaagggattc agtacactat tgatatgaaa
     7621 cagcctgttg gacaacggat taccgaactt cgttttaaag gtcaccccgt tcttccgcaa
     7681 caaatgctgg aagtggtgac aaatcaatat cgggcaatcg gcggaggaaa ttatgcgatg
     7741 tttggtgcgg agaaaattgt ccgagaaatc caaatcgaca tgaccgaatt aattgccgac
     7801 tatttgaata ttcacccacg catcgaagca aaagtgaatc ataattttca agtgattttg
     7861 tagtatataa ataaaaaatt caaaaaggtg tgagcagttt tttgcccaca cctttgttat
     7921 gccaaattgt taggctgtct gatttccatc cagccattct ttagccagcg atctacctca
     7981 tcaaaacaaa aaattttcct ctgatggttc cttaaataag actattttga tcctttttcc
     8041 atgaataaga ttttgcatca gacaatactt catttctgaa aaagttgcgg caccaatata
     8101 gcctaatacc cctttccgat cttgattaaa tacaaaaaat aaatccgctg ctgcgatatt
     8161 taaaaaaaag tcgtcaagat ttctggatac tctttgtcta ggatcggaca tgagcgagga
     8221 taatcaatta attcaatgcc ttttttactg aaatcttttt ccatatctcg atattcttct
     8281 tggaaaacaa tacttccaga aataacagct ttcacaaaat tggcctccat ttttttattt
     8341 ttcagacact ccaccatacc ggatacctct caaagaaaag acatagcaaa aatcaatagc
     8401 agctatctct ccacggtttt ttactggtaa ttttattttt cttttggaaa ttgtatcagg
     8461 aaggtcgttc cttcattttt tcggctggtc acttcaattg aaccgtggtg caggcgcact
     8521 agttccttca cgattgataa tccaagacct gactcgccta tctttgtatt tttacttgac
     8581 ggatcggctt tataatagcg atcccaaatg tttttttgtt cctcttcaat catgccgatg
     8641 ccattgtcct cgacagtaat aaatacaggc tctttgtcaa atgatgttgg taaagagaaa
     8701 tcttagaata atttggacag aacagaaaga agaaactctc tttctgttct gtttatttgg
     8761 ttaaaaatgt tttgccatga cgaatcatga tccgtagttt gaaattgtaa aagctgcgaa
     8821 agccgtacgc attgcgtttt aatactttga tgtggttgtt taaacattcc aatggtccat
     8881 ttgaatagct gtagtgaagc gcattttgga tctgtggcag aaacttttta aacgttcgta
     8941 aagtggttgt ataggtttca ggaagaccgc tgacttctct gtttaaacag tcctcaaata
     9001 atgactgatc tcttttcttt accgcataaa gaatgtcttg gtataactga tagccttggc
     9061 gcaggttggg acaagcagat aacagtcgat cgacaatctc actttcagtc aaataatctt
     9121 taaaactggc acgccaaacc cttttttcgt agtttaactt tgcttcttct ttttgtagga
     9181 gtttccaata gcgtttcaat tttttcccta accctcgttg agagtgggaa gttcctttaa
     9241 gccgtgccgt ggtttctttc actcgatgat tcaagaaggt tctgccaata tgttggacga
     9301 tgtggaaacg gtcgatgatc aactgagcat tcgggaaaag ctttttcacc aatgaaacat
     9361 aaggcgcata catatcaata acaatccatt gaacggcttc acgagtcgct aatgaaaacc
     9421 gagaaaaata gcattctaaa taaggcaagc gtcgattttc gacaatatcc aataatgcat
     9481 ggctttgtcc gtccatcatg ataaaactca ttgcactggc aacacttcga accgatttga
     9541 attcatcaaa gcataagacc tttggcaaag cctgtttgaa aggattcaat gggcggtaga
     9601 atcgcttcat gacacgatag accgaagaag tggaaatatt tttctgacga gcgatttccg
     9661 tcatggaagt attctctttt aagcgagcaa tgacagacca acgaactttt tgactaatga
     9721 aacagtgttt ttcagcaaca gaggtatccg ctacgaatgt tctttggcaa gacttacatt
     9781 taaatcgttg tttcttcaac tccagataag tgcgatactc agacgcatca ttcagtaaca
     9841 accgaacggt tgtcgtcccc caacgaatga tcctagaggg gacgacacaa agacaatggg
     9901 aacactcttc tggcttgtac agaagagttc ctttgtaaat catggatttg agtcaatatc
     9961 ttggacacat tttagtagag acggttaagc cattttttct tttgctaatt cttcaaattc
    10021 ctgtggggtt agatagtgaa tcgagctatg gattcgattt cggttataaa atccttcaat
    10081 gtagctaaat agcgctcgat ttgcttcttc aaaatctgag tagctagtcg tgtagacttc
    10141 ttcttttttc aatgaggcat ggaaagattc gattccagca ttatcataag gtgttccttt
    10201 tctactataa gaatgcctta ttttgttggt tttaagccat tgttccactt ctcttgctgt
    10261 gtattggctg cccaagtcag tgtgtagaat cattccttct ggaatgtgat aatgtatttt
    10321 tgctttattc aatgtttgaa tgacacaatc cacagtcatt cgttttgaaa atgtataact
    10381 aataattttt ttcgtgtgta aatccatgat tgaagataag taacaccaac catttttctt
    10441 agtaggaatg tacgtaatgt cagctgccca tttctcacag atcgtttcag tagaaaagtc
    10501 ctgatttaag agattctctt ttgaaatgat cggtttatta gacctttgag gtctatattt
    10561 tttaactaca atcgacctta aatttaattg tttcattagc ttttggacat gtttaagaga
    10621 tactgaaatc ccttctttta ataaaacttg atggatcttt gtggcaccat agcgttgttt
    10681 attcgtaaaa aaaattgttg aaatcttctt tttcaactta ttatttctga tttctgtagc
    10741 tgtcaacggt ttattcttat aaaaataata gacacttcta gggatttcta ataaccgaca
    10801 taacaaagaa acagtgtaat ccttacacca tttttcaata aattgaacaa gcgaatttag
    10861 gttttccgag agaatatggc tgcggctttt tttaagattt caagttcctc atttaaacga
    10921 gcattttctt ttctcagttc agctacttct tttccagtca gtcctgtgga ctgatcaggt
    10981 aaatataaat tcttccattt gtaaatcgtt gcttcagata agccatattc tttcgctaac
    11041 cctcgaacag aacgtccagt ttgattcagt tcaacaatca tttgtttaaa attttcttca
    11101 tatcgggtca taaaaattca tcctctcgta gataagttta atttattctt gtctctacga
    11161 aaagctgtct aagatttgat actaacacca agttatcttt agaaccattt cagatacata
    11221 tgaaacaatt acatatgatt ttttcataaa aaaacagtac tgtctagtag tataaaatat
    11281 acaaatttct taaaaatttt ttcactaaaa tcactacctg aaacagtcta aaacacttat
    11341 atatcaacgt ttaatgaaac tatctccgtt tcatttcaga tacaaataaa tatagtcggg
    11401 ctccaaatcc gaaaaaaaat acccccagta gcttatccta agctactgga gatgtataag
    11461 tacggtcata taacaacggg gtagggtaaa taaatgaaaa agatggaaga atcattattc
    11521 aataactttt ttaaatactt cgttgtaatc agttacctgt ccgttcttaa ataccacaga
    11581 ataatctttt ttcccatttt caagtaaccg aagtggaatg tctttaccaa cttgctctgt
    11641 cactgcatta ctgatacctg ttagattagc taataaatct gataaagatt gagcatctgt
    11701 agaaatctct gtatcagtat ttatcgtgat tcctttatct tcatttacaa gcttccaagt
    11761 aggtggtata gctgtgtatg tttcgatgaa tgaatcaatt ctttcatcta tcgtcttccc
    11821 agtatctact tctgtctcat tactcttaga tgaatcgatt ctttcatcca tcgtctgctc
    11881 cgtaactgct tctgtctcat tattctcacc ggaaactctc ttcacaccca aacttatata
    11941 ttgtccatct ttagtgtgca tagttaaaat tcccacgctt ttactaataa attcactata
    12001 accagttctt tcaatagaga cttcaatgta tttcacacta tcatactcaa aagaaaaata
    12061 gacatgtaaa agcattttgt tgtttacatc tacataataa tcctttgact ctacttgaga
    12121 ccttcctata cggaatacag ctttactgtc tgatatctct ataccagcat ccttattatt
    12181 aatctgccaa tctccaagta gctcttctgg caagctagta gtttcttgtg aacactttac
    12241 cacttcactg gtagaggtcg aaacattcac ctccgcactg ttagaagaat tttccaagga
    12301 atcaggagcc ttagcacaac caacaagtct ttatatattg tgcagccgat attttcttca
    12361 ttcagtacac ccttgaaata tgcagtgtta gataaagcag ttcgtcctga tttgctgagg
    12421 cacccatctc ttcttgaacg tactccttga ttttcaaggc cgcccgatac tctttcggaa
    12481 aacgctgctt cactgaagcc agcaaaaaat catccttctc cggcaactct tccttcttaa
    12541 tcattcgggc aataaaaaac tgtaaatgcg tcacaaatct taagtaaagc atcgaatctt
    12601 tatcaacctt tttttcggga aacagatatc ctagcatagc caagatatcc tttagtgctc
    12661 ttaccgagag catggtttct tccatatttg cttgttcact ttgggcattt acaatgtgaa
    12721 acgcaatgtt tccagcctcc tcttctggca actgaacatt catttttttg ttgatcaatt
    12781 ggatagcata gcagccgatc tcaaattctt ttgggtaata cttttgaatt tcaatcagca
    12841 ggcgattttg aaaaacaatg cctttttgac tgcgttcaat ggcatgcttc aagtgatcaa
    12901 ccaaggtaaa aaatagactt ttattcagct taaagtcaaa cttggcgttg gcataatcga
    12961 taattcgctt gacgcaaata attagatcat ccggagcagc ctcaaacagt ttaatgtaat
    13021 ccttttttag agcatcagaa agtgaatcta atttaaatac tttttggatt ttgtcagccg
    13081 gaaccaaatc gcctttcttt agttgaaatc caatcccttt ccccatcaaa attacttctt
    13141 tgccttgctt atctaagcaa agaattaaac tgttgtttaa cgttttaatt actctcatta
    13201 gattcaccgt tctctctaag ctaagccatt tcttgcgatt actttttgat accaaaagaa
    13261 gctttctttt tttattcgtt ttaattcttt aagattctca tcggttcggt caacatatat
    13321 aaaaccataa cgcttattga agccttgatg ggtactaaca acatcaatcg ctgaccaagg
    13381 taaataacca aaaacatcta caccatctgt taaagctgct tgaatagcca gaatatgttt
    13441 ggctagataa tcaattcgat cctggtcatt aattcgtcta tcttcctcaa ctatgtcggg
    13501 tgccccatag ccattttcag taattaaaat aggtaaatca tagcgatcgt acaatttcct
    13561 caatgttaat cgtagtccag tagggtcaac tacccagcca tatggcgttt tatctacata
    13621 aggattttca gctgcccggt agacaccttc ttctcccaac atgatctgtt gatcccccgc
    13681 tctagcggaa acatctgatc cgtcattttt gctggcagcg atggtggcag ttgaataata
    13741 attaatcgca ataaaatcgg gtttaccgga ggataaaatt gcctgatcct ctgcctcaat
    13801 tttaggatat aatccacgat cattcaaata gttttcaaac aaataattgt attttccccg
    13861 aactgctaca tctaagaaag accagcctct caatgtttcc caattatggg ctgcaattgc
    13921 atcttctgga ctggaggtag cttgatacat agccgttaaa ttgattgctg gaccgatttt
    13981 tcctttaaga ttcaactgat gatacaagtg aattactcta gcttgagcta ataaaacatg
    14041 gtgattaatt tgaaagagtt ctttttttga aggcagctct ccacttggtg gcaaaccaat
    14101 cgctccagga tgaaggatca tcgtgttttg ctcattaatt gtcagccaat agttcacctt
    14161 gtcaccatac tcagtaagca atacctgtac atagtccaca aaataatcga tagttgctcg
    14221 atttaaccaa ccgccctgct cctctagaaa ataaggtaaa tcgaaatggt acatggtgac
    14281 aatcggttcg attccctgat tcaggcattc atcaattact tgatgataaa aagaaattcc
    14341 cttttgattg gttttattat tttcaatatc cataattcgc gtccaagcga tggaaaagcg
    14401 aaaagctttt aagcctaatt ctttaaacag ctgtatatct tctttatagt gatggtaaaa
    14461 atccgaggca acttcaaaat tagctacccc ttctgtatgc tgatacttgt caataataga
    14521 cggttgtttc ccatcctcct gataggcacc ctctgcctgg taggcactaa tggaggcacc
    14581 ccataaaaac ttctctggaa atgctgaaag ctgtttgtat tgcacaaaaa tcctctcctt
    14641 aactaatttg catcaatggt tcgtgttcct ttaagcttcc gcttgcttca ctaattcctg
    14701 cgtaattttc actgttggtc actaccacaa tcactgtatc atcgtagcca gcgtttctaa
    14761 tggcagcgaa atcggcgttg attaataatt gtcctgctga cacctgatct cctgctttta
    14821 caaatacttc aaagggttcc ccttttaaat tcacggtgtc gatccccaca tggatcaata
    14881 attcaatacc ttctttagaa cgtagaccaa tcgcatgttt agattctaaa atagtaatga
    14941 cttctccctc aacaggtgaa taaatgctgt tttcttctgg atgaatggct acgccctttc
    15001 ccaatagtcc cttagaaaag acagcatcgt tcacttcaga aataggacac acggttcctt
    15061 ttaccggatt ttcaacaatg actgcttcgg ctattgccgt ttgctctgat tcatcagaag
    15121 tatcgtcaaa tccaattaga taagcaatga taaaggttgc aacaaaggcg attacacacg
    15181 taataattgc gtgaacaata ttcattggat tttctccaat aaaggctggt aaggctgcta
    15241 agccagggga aacaaaggca tatctgacta aaccgctcaa gccagcatac aaaccagcaa
    15301 ttgcaccacc aatcatggaa gcaactaatg gttttttcag cttcaaggtt actccataga
    15361 gtgaaggctc agtaattccc attaaagcag tgatcccagt ggacacagct agctgcttca
    15421 actctttgtt tttagttttc gcggctacag caaaggtagc acctgattga gcaatattag
    15481 aagccagcat tcctggccca ttgatcattt cagaacccgt actgctcaat tgcatagtag
    15541 cgattggcgt cattgcccaa gctgtacccg taaccaccaa aaatggctgc aaaccaccca
    15601 tcaaaaatgg aattaaccaa gctgctttgc cgttaatcca ttcagccccc attgctataa
    15661 gatcgttcag caacgttcca aacggtccaa tgacaaccaa cgctaaaggc cccataatta
    15721 aaaataccag catcggctta gtaaagaatt taataaacga aggcgaaatt cgatccgcaa
    15781 agggctctac ataggacatg atccaaacag taaagataat cggtaaaact gatcccgcat
    15841 aatctgcagc taataccgga attcctagaa aagaaacggc atcgccagta gaaaaaagct
    15901 gtgaaatatt tggatgaagc aaaatgccag caacagtaat cgccaaaatt gggttgacat
    15961 caaactttat ggctgcccca taggctaata gtactggcat aaaataaaag gctgcatcag
    16021 caatagtatt caaaatgatg taagtttgac tactgtctga tagtacacca gtcaaagtca
    16081 agacggccaa aatggctttt accatcccgg cacctgttat agctggaatc atcggtgtaa
    16141 aggtggtaga aatgacacta atcaatcttg aaataatact ttcttgattg tctgaagagg
    16201 ggcccttttt aattgaagtt cccacttcct tttgaatttc cttgaaggct gtttggactt
    16261 cattgccaat caccacttgg tattgtccac cgctatccac cacactaatt acaccaagtg
    16321 ttttttctaa ggctttcgta tcggctttcc ttttatcttt gagttcaaaa cgcagtctgg
    16381 tggcacaatg ggttaagctg accacatttt cttttccccc aacctgttgc aatacatttc
    16441 ttcctacttc tctgtaatcc attattctct ctccttaaag cagtaaaaaa gaccaagtca
    16501 aaccacgccc caatcaacat tgaagtgtta gtttgtcttg gtcttgcctg cataatcagt
    16561 aacatgccaa taacgttatt ttgctgtaat ccaataatag cacattatga tagcgcaatc
    16621 aacagggaat ttaatttttt atcttttcgt ttgaccgtat agggtaatat aacagttaga
    16681 tcacatcaac tagggtatat ctgcgtatac ttactgctaa aataagtata tctaatatta
    16741 actataccag taaaaccatt cctacataac cagcgggaaa ttcccaaaat cgtgaaagca
    16801 tcaaaaatgc atataacgga tcacacaact tcaacaattc tgctcttggg aaaaattgat
    16861 gaatttggtg agtaaaggat ggaataaaca gcattccaac aatataggca acaatagtga
    16921 tacttgtttt tttggtaaaa atcagtaagc aatagccaat ttcaatcatt ccgagatata
    16981 aaggtatcga tagaatgatt tgggctagta ttcttaaaaa ttaactagca aatgtttaaa
    17041 ctgatttatt tctaatctac atgtagtaag tatttcaatt ccaccaatta tcaataaaaa
    17101 taccaaacat atccataata attttttctt tttcatttct tttatattgt tataacagtg
    17161 aataacataa aaattcccga caatcattgt aatatttttt tgtcacctgt attagtattt
    17221 aatttatagg aaactataaa aactccctag tgaatttaca cctttgggag ttcctgtttg
    17281 gacggtatgc ataatacatt taaatcaatt taacattaac acctcagcat atggtatctc
    17341 cacatatata cggtctttct ttggatagta ctcaatattt atgttgatag cacttccatt
    17401 gtctaatata gctttagccc ggtatgtgtt atctccattt tcatgagtaa tcgtcacatc
    17461 atcacatgat cgatttaagt tgtactgttc ttgtaaaatc tgtgttacca atggtttcga
    17521 tgacttctct aatccagatg tacctgtcat ttttgaaaaa atgataccta taatagccaa
    17581 gacaacgata actataatga taaacttctt gttcatattt actttattca actttttatc
    17641 tcctatctcc aaaataattt gctcttatcc ctcttatcag tacattgaca tccctaaaag
    17701 tacaccaaag attgctccag ctacccctgc tatcatcaaa atcgtaccca tttgttcatc
    17761 tttatctcga tataagtagc ccataataac agcaacagca ccaaatacaa caggaataaa
    17821 tgctaacgct actaccatag aaatccaacc aataatcaaa tacagcttcc ctgtctcttg
    17881 tccatgtacg atttgttcct tcatttcatt aattgtctgc tcagtacttt tctttactgc
    17941 gtctgctcca gttgtctcat ctgtttcttg tagtgctccg caaaactggc aatacttact
    18001 ttcatctgtt atttctttgc cacattttct acaatacatg tcttctcctc ctaaaaacat
    18061 tcaattgttt attagttaac tgtttacttt attaaataca tcagaaaaca gctcattcat
    18121 tggtacactt aatatctcag aaagcatctt catttgatag ggtttaggaa tcgttctccc
    18181 tgtttgccac gaacttacac attgctgtgt aacattcatt tttttgctaa ctccgtttga
    18241 gaaagtcctt tttcttttct gtatcggctg agattactta cttttttcat gattctcccc
    18301 tcctgtttta ttatatacaa caatatgatg tatttcaact cataaaatat aaaaaaactt
    18361 caaataccat tacaaataca acaacttgtt gtactatact gagatggggg tgtattaatg
    18421 aaacagaaac aatatgcaaa ggtcattaga gaaaagcgaa aagctctcgg gtggacacaa
    18481 aaagaattag ctgaaaaaat cttctctaca caacaggcag tagcaagatg ggaaaattct
    18541 gtaacagagc ctaacctcga tagcttaacc gctctttcta gagccctagg aacccctgta
    18601 agccattttc tagacaatgt tgtcgtagat tatgaggaag agtttttggc tctctatcgt
    18661 tctcttagca ctgaagatgc agttcgaaca attgactata tgaagctatt aaaaaggcaa
    18721 gaaaatgaac gcaatcagct attgaaagat tgaaaaataa ataacccacc gaactttaac
    18781 ggtgggtgta ttgagatgaa ataagattaa gagctatgga taacctgtct agtttaatac
    18841 aattgggtct tttctcttcc ttactctccg tccatcatct cttgttacac ttgtaaattg
    18901 ataaagagga actaattcaa ctcctgataa tatcaattct tttattactt catctatccc
    18961 tgctcctttt gctaggtaga ctaaaaaact actacttgct ttcacattgt atagtctctt
    19021 aatatcctta gaagctcgtt caagagtgaa tccttgttta acaaagtagc ctctgttttc
    19081 ttttaaagca ttttccacgc tgttattccc aacattacgt agacgttcac aaacagtgtt
    19141 agtaagttct ttctctgtca ttagatatgt tccttgccac gataagccct gtaaaacttc
    19201 atgtgcttca tctaaaatca attgtttttc cttgctaagt tctgatcggg aattttgagg
    19261 aaatactctt ttagcctctt tttcgttaaa acatatctta acccattcat aagaaaaata
    19321 ttgatatgta aaatgaaatt tttccagaag tggaaggata tcttcattca atttttgaat
    19381 cacagaagtt gcattaaaaa cgtctaatcg atagacattg cttctcttat gattaaagtt
    19441 tcctaacgct ttctcttcct ttaacctatt taggtattcg agcatcttca aaggaacatc
    19501 ttcatcatct aattttttta tcaatcctat aaaagtcata agtgatagta acttattaca
    19561 tttatctatg tcataagttt taccatagta aaaactcatt tttttagcta attgttcccc
    19621 cgacaaaata caagtcacac tttccttact atcatcaaaa aaattaacat cagaaagcaa
    19681 gacatcatag acacgtcgca tttcctctag ataattcctt attgtcctga aaagtttgtc
    19741 tttcattgat tcaagagtgt caaaaagtac ttttgattcc tctttcaatt ttcgaaaatg
    19801 tgtaggatac aagtctaggt tctctaatag ccaattaaat gtttctaccc gactgtcttt
    19861 ctttaaaatt agcatcacaa ctccaataaa attttcactg atatcacaat tactggaatg
    19921 acaataaaaa atggaatgcc cagttttact ggtaaaaata ctagcagagg gattatgatc
    19981 gttgtgaaac agacatctta agtttttact tgacttaagt aatttattca tatcaatggt
    20041 taacaatctt tcataaatct cattaaaaga catgtcggag gaatcaaaca ggcagtcttt
    20101 aaaccactgt ttcatttctt ctttgtcgtc attttttatc aacttaacaa aatcggtttc
    20161 attgactagt tcatactctc tccctgaatc actttgacaa tttttagaac atgtcatggt
    20221 aaaatcaatc ggaagctcag taatatctaa catgttgtct tcattaatta ttatcactgc
    20281 atctgactta tatcctccaa aaaacattct attcgaagca tttacattgt catcacaccc
    20341 ggggatgttc tttttcaagt attcgtaaac agcaaccatt ttaccatagt cagtaattgc
    20401 ttcttttaat agaaagacaa tcttgtattt ttctagattt tcagagtacc gaatactttt
    20461 ttgtatcgca aacgcagaat tatgaactaa attgatagat aaaacctttt ctatgcttaa
    20521 gtatctacta tcatctttac tcaaatctac taccttcccc ttctcatctt taacagtgtt
    20581 atcgacatcc agaataatta ctctggtttc cacaaaactc tccttgtcat tgcctttatt
    20641 ctttgtcttg cttggaataa atgattgtga tgaatttagt atctctacaa actcatctct
    20701 tgtaacttct ctatagttat ctaaacacct atctcttaca tcagtttttt ctttttttgt
    20761 aactggcttc tctaataatt tttctttatc tacataaatt ggaatattaa tcattgatgt
    20821 ttccatttgt gtttc
//
LOCUS       DAJSKS010000040        20631 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJSKS010000040 DAJSKS010000000
VERSION     DAJSKS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.041"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            292..981
                     /locus_tag="ON396_001238"
     CDS             292..981
                     /locus_tag="ON396_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3113334.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            968..2176
                     /locus_tag="ON396_001239"
     CDS             968..2176
                     /locus_tag="ON396_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3113335.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2470..3741
                     /gene="serS"
                     /locus_tag="ON396_001240"
     CDS             2470..3741
                     /gene="serS"
                     /locus_tag="ON396_001240"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR3113336.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4290..5774)
                     /gene="guaB"
                     /locus_tag="ON396_001241"
     CDS             complement(4290..5774)
                     /gene="guaB"
                     /locus_tag="ON396_001241"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR3113337.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6279..6974)
                     /locus_tag="ON396_001242"
     CDS             complement(6279..6974)
                     /locus_tag="ON396_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HCR3113338.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7064..8164)
                     /gene="ychF"
                     /locus_tag="ON396_001243"
     CDS             complement(7064..8164)
                     /gene="ychF"
                     /locus_tag="ON396_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR3113339.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8173..8364)
                     /locus_tag="ON396_001244"
     CDS             complement(8173..8364)
                     /locus_tag="ON396_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCR3113340.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8385..9275)
                     /locus_tag="ON396_001245"
     CDS             complement(8385..9275)
                     /locus_tag="ON396_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR3113341.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9262..10029)
                     /locus_tag="ON396_001246"
     CDS             complement(9262..10029)
                     /locus_tag="ON396_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3113342.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10041..10754)
                     /gene="rsmG"
                     /locus_tag="ON396_001247"
     CDS             complement(10041..10754)
                     /gene="rsmG"
                     /locus_tag="ON396_001247"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR3113343.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(10986..12887)
                     /gene="mnmG"
                     /locus_tag="ON396_001248"
     CDS             complement(10986..12887)
                     /gene="mnmG"
                     /locus_tag="ON396_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR3113344.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12904..14301)
                     /gene="mnmE"
                     /locus_tag="ON396_001249"
     CDS             complement(12904..14301)
                     /gene="mnmE"
                     /locus_tag="ON396_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR3113345.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14600..14983
                     /locus_tag="ON396_001250"
     CDS             14600..14983
                     /locus_tag="ON396_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113346.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15151..15909)
                     /locus_tag="ON396_001251"
     CDS             complement(15151..15909)
                     /locus_tag="ON396_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HCR3113347.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15927..16745)
                     /locus_tag="ON396_001252"
     CDS             complement(15927..16745)
                     /locus_tag="ON396_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR3113348.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16742..17098)
                     /gene="rnpA"
                     /locus_tag="ON396_001253"
     CDS             complement(16742..17098)
                     /gene="rnpA"
                     /locus_tag="ON396_001253"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR3113349.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17216..17350)
                     /gene="rpmH"
                     /locus_tag="ON396_001254"
     CDS             complement(17216..17350)
                     /gene="rpmH"
                     /locus_tag="ON396_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR3113350.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17874..19208
                     /gene="dnaA"
                     /locus_tag="ON396_001255"
     CDS             17874..19208
                     /gene="dnaA"
                     /locus_tag="ON396_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR3113351.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19406..20536
                     /gene="dnaN"
                     /locus_tag="ON396_001256"
     CDS             19406..20536
                     /gene="dnaN"
                     /locus_tag="ON396_001256"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR3113352.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5906 a         4211 c         3794 g         6720 t
ORIGIN      
        1 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
       61 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      121 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      181 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      241 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      301 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      361 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      421 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      481 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      541 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      601 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      661 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      721 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      781 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      841 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      901 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
      961 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1021 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1081 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1141 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1201 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1261 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1321 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1381 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1441 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1501 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1561 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1621 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1681 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1741 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1801 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1861 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1921 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     1981 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2041 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2101 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2161 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2221 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2281 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2341 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2401 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2461 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2521 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2581 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2641 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2701 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2761 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2821 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2881 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     2941 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3001 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3061 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3121 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3181 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3241 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3301 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3361 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3421 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3481 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3541 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3601 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3661 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3721 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3781 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3841 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3901 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     3961 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4021 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4081 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4141 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4201 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4261 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4321 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4381 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4441 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4501 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4561 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4621 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4681 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4741 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4801 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4861 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4921 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     4981 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5041 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5101 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5161 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5221 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5281 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5341 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5401 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgcggc
     5461 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5521 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5581 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5641 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5701 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5761 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5821 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5881 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     5941 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6001 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6061 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6121 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6181 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6241 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6301 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6361 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6421 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6481 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6541 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6601 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6661 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6721 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6781 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6841 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6901 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     6961 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7021 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7081 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7141 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7201 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7261 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7321 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7381 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7441 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7501 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7561 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7621 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7681 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7741 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7801 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7861 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7921 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     7981 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8041 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8101 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8161 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8221 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8281 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8341 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8401 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8461 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8521 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8581 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8641 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8701 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8761 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8821 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8881 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     8941 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9001 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9061 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9121 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9181 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9241 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9301 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9361 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9421 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9481 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9541 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9601 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9661 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9721 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9781 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9841 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9901 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
     9961 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10021 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10081 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10141 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10201 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10261 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10321 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10381 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10441 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10501 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10561 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10621 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10681 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10741 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10801 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10861 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10921 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    10981 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11041 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11101 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11161 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11221 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11281 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11341 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11401 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11461 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11521 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11581 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11641 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11701 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11761 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11821 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11881 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    11941 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12001 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12061 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12121 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12181 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12241 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12301 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12361 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12421 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12481 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12541 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12601 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12661 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12721 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12781 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12841 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12901 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    12961 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13021 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13081 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13141 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13201 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13261 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13321 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13381 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13441 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13501 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13561 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13621 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13681 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13741 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13801 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13861 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13921 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    13981 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14041 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14101 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14161 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14221 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14281 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14341 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14401 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14461 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14521 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14581 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14641 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14701 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14761 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14821 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14881 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    14941 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15001 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15061 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15121 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15181 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15241 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15301 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15361 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15421 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15481 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15541 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15601 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15661 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15721 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15781 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15841 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15901 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    15961 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16021 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16081 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16141 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16201 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16261 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16321 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16381 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16441 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16501 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16561 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16621 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16681 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16741 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16801 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16861 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16921 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    16981 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17041 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17101 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17161 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17221 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17281 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17341 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17401 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17461 aacttatcca caagattatt tttttctatg ggagtcctat gtggaaaaag ttatgcacag
    17521 ggattatcca cagataataa atggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17581 aatccacaat caggtgattt cctgtgtatt ccgtctccta actattggta tatctatatt
    17641 ttttatccac agatgcaaga ggaaatctat tgatttttac gagttttcca cctgtgcatt
    17701 agtggtgtgg aaaacttttt tttgacaact gtgtttttct cttttttctt tttgtcgatt
    17761 gtgtggataa ttgtaattaa aaaatttttt tcttcggttt ctgtggaaaa ctattgaaaa
    17821 aaatgctaaa ttagtactag ttattcactt tttaatgaag gaggaggcct tatatggtat
    17881 ccctcgatgc tttatggaat gaattaaaag caacatacca aaaggattta tcgcctgcta
    17941 gctataatac atggatcgaa acagcgaacc ctcgaaccct tgatcaaaac cagctcgttg
    18001 ttgaggttcc tagcaagatt cataaagaat attgggaaaa aaacctggcg acaaaaatcg
    18061 ttgaagtcgg ttatatgtta tcaggaaatg agattatccc tcgctttatt actggcgaag
    18121 aagcagagca agaagaagtt atagaagaaa aaaatccaaa agttgtggca cctagtccgc
    18181 taaaaaaagc catgctgaac cctaaatata cctttgatac gtttgtcatt ggtaaaggaa
    18241 accagatggc tcatgcagca gcgctcgttg tagcagaaga tccaggttct atttataacc
    18301 cgctcttctt ttatggaggc gtgggacttg gtaagactca cttgatgcat gcaattggtc
    18361 atcaaatgct gcaaagtcag cctaatgcca aagtaaaata tgtaagcagt gaaacttttg
    18421 ctaatgattt catcaattcg atccaaaaca aaacagcaga agagttccgt caagaatatc
    18481 gaaatgttga tttactgcta gtggatgaca ttcaattttt tgcagaaaaa gaagctacgc
    18541 aagaagagtt tttccatact ttcaatgcct tgtacaatga agggaaacaa atcgtactaa
    18601 ccagtgatcg gttgccaaac gagattccga aactgcaaga acgcctcgtt tctcgctttg
    18661 catgggggct gtctgttgat attacaccgc cggatcttga aacaagaaca gcgattttac
    18721 gtaaaaaagc ggcggctgaa cgtctagaga tcccagatga cacactaagc tacatcgcag
    18781 gacaaattga ttctaacatt cgtgaattag aaggtgcgct tgttcgagtg caagcttttg
    18841 ctgcgatgaa tagtgaagat atttcgacta gtctagctgc agacgcactg aaaacgttaa
    18901 aatcaggaaa aggacatcct caattgtcaa tcctgcagat ccaagaagaa gtggcaaagt
    18961 attatcacat ccaattgaaa gatatcaaag gaaagaaacg cgtcaaatct attgttgttc
    19021 ctagacaaat cgctatgtat ttggcacgtg agctcacaga taattcttta ccgaaaatcg
    19081 gagctgaatt cggtggcaag gatcatacaa cggttatcca tgctcatgaa aaaatccagc
    19141 agctgatgga ttctagtgtc agtatgcaaa atgaagtatc agaaatcaaa aactttttac
    19201 taaattaatt tgtcgattct atcaactgaa aatagcatgt ggataaaaga aaaaaacgtt
    19261 caaaagttat gcacactttt tccacagctg ttttttcgtt tctctcttac gttttttgag
    19321 ttttccacag aatcaacatg ccctattact tttattattc tttttaacta ataataaata
    19381 aacaaacatt caaaggagat acatcatgaa agttacttta aaccgagcta gctttatgca
    19441 ggaattgcaa actgttcaac gagctatttc aagcaaaacc acgatcccta ttttgacagg
    19501 tgtaaaaatc acactgacac aagaaggttt gactttgacg gggagcaacg ctgatatatc
    19561 aattgaaact tttttgtctg ttgaaaacga aaaagcaaat atgcaaatcg aatctactgg
    19621 ttccattgtt ttacaagcac gtttctttag cgaaatcatt cggagacttc ctgaagaaac
    19681 atttacttta gaagttttag aaaataaaca agtagcgatt acttctggaa aagcgaattt
    19741 tatcgtaaat ggattagatg cagataacta tcctcatctt cctgttgtcg aaagccataa
    19801 ccagatgaaa ttacctgtac acgtattgac taaactaatc aacgaaacag tttttgctgt
    19861 ctcacaacat gagagtcgtc caatcttgac aggtgtgcat ttcattttat ctgataattc
    19921 tttattggct gtagctactg attctcaccg tctaagtcaa cgcgtgattc cagtagaaca
    19981 agcggctgat cattttgata ttgttattcc tggaaaaagt ttgatcgaat tatctcgctc
    20041 attaacaaat gaagaagaaa tcgtcgaaat cagcattatg gaaaaccaag tgttgttcaa
    20101 aacagaaacg atgtatttct attcccgttt gctagaagga aactatcctg ataccaatcg
    20161 tttgattcct tcaagtttta atacagaggt tgaattttct gttcctagct ttttagcggc
    20221 gatcgaacgg gcttctttac tttctcatga aggccgtaac aacatcgttc gtttatctat
    20281 tcgtccagat gcggttgttt tatacggaaa ttcgccagaa atcgggaaag tagaagaaag
    20341 cttgagttat acagcaagca gtggcgatcc attagatatt tctttcaatc cggattatat
    20401 gaaagcagcg ttgcgtgcat ttggagatat gagcatcaaa gtgaaattta tctctgctat
    20461 tcgtccattt acattggaac caacggaaga tggcgttcaa tttattcagc tgattactcc
    20521 tgtacgtaca aattaacaat gattttcatt gttcttgaaa atttaattga aaagaaatag
    20581 aaaaaataaa attttaaaca gaaggcccta aaatctcttt taaggctttc t
//
LOCUS       DAJSKS010000041        20363 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJSKS010000041 DAJSKS010000000
VERSION     DAJSKS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.042"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            292..1431
                     /locus_tag="ON396_001257"
     CDS             292..1431
                     /locus_tag="ON396_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCR3113353.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="ON396_001258"
     CDS             complement(1498..2073)
                     /locus_tag="ON396_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3113354.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="ON396_001259"
     CDS             complement(2078..3052)
                     /locus_tag="ON396_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCR3113355.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="ON396_001260"
     CDS             3335..4303
                     /locus_tag="ON396_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3113356.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="ON396_001261"
     CDS             4416..4694
                     /locus_tag="ON396_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR3113357.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="ON396_001262"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="ON396_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR3113358.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5151..5906
                     /locus_tag="ON396_001263"
     CDS             5151..5906
                     /locus_tag="ON396_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3113359.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="ON396_001264"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="ON396_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR3113360.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="ON396_001265"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="ON396_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR3113361.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="ON396_001266"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="ON396_001266"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR3113362.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="ON396_001267"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="ON396_001267"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HCR3113363.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="ON396_001268"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="ON396_001268"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR3113364.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="ON396_001269"
     CDS             10643..11185
                     /locus_tag="ON396_001269"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR3113365.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="ON396_001270"
     CDS             11208..12764
                     /locus_tag="ON396_001270"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR3113366.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="ON396_001271"
     CDS             12778..13680
                     /locus_tag="ON396_001271"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR3113367.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="ON396_001272"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="ON396_001272"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR3113368.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="ON396_001273"
     CDS             15123..15545
                     /locus_tag="ON396_001273"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR3113369.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="ON396_001274"
     CDS             16014..16244
                     /locus_tag="ON396_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCR3113370.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="ON396_001275"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="ON396_001275"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR3113371.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="ON396_001276"
     CDS             17655..17831
                     /locus_tag="ON396_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR3113372.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="ON396_001277"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="ON396_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR3113373.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18709
                     /locus_tag="ON396_001278"
     CDS             18326..18709
                     /locus_tag="ON396_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3113374.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18878..19762
                     /locus_tag="ON396_001279"
     CDS             18878..19762
                     /locus_tag="ON396_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3113375.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19876..20112
                     /locus_tag="ON396_001280"
     CDS             19876..20112
                     /locus_tag="ON396_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HCR3113376.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="ON396_001281"
     tRNA            20200..20280
                     /locus_tag="ON396_001281"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
     gene            20287..20358
                     /locus_tag="ON396_001282"
     tRNA            20287..20358
                     /locus_tag="ON396_001282"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20319..20321,aa:Gln,seq:ttg)
BASE COUNT         6760 a         3567 c         4425 g         5611 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga atggcgcaaa
    11401 acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag gccttgatcg
    11521 gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgattgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcaccattac tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca gcaaaattaa
    12121 gtgatgatct aggcggcggt tcaatgactg ctttgccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tctcaatcac tgacggacaa atcttcttag
    12241 aaagcgactt gttctacgcg ggtactcgtc cagcggttga tgccggacta tctgtctctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    12361 acttagcaag ttatcgtgaa ttagaagcat ttacacaatt cggttctgat ttggatgcgg
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaactgc
    12481 atgcaccgct agctgttgag aaacaagtag tgattttgta tgccttgaca catggattct
    12541 tggacagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatt tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aaacgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg tgaagaaaac cggttatatc gtcatcactg cagatggcgg tctggtcggt
    13081 ggatataaca gttcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaacc tagcatatga gctgcgcaat ttatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaatcg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtggg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataactgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattaat ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
    20341 cgaatccagc taccccagta tac
//
LOCUS       DAJSKS010000042        19835 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJSKS010000042 DAJSKS010000000
VERSION     DAJSKS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.043"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ON396_001283"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="ON396_001283"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR3113377.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ON396_001284"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="ON396_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR3113378.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ON396_001285"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="ON396_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR3113379.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ON396_001286"
     CDS             complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="ON396_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR3113380.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ON396_001287"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="ON396_001287"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR3113381.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ON396_001288"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="ON396_001288"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR3113382.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="ON396_001289"
     CDS             complement(8557..9027)
                     /locus_tag="ON396_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCR3113383.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="ON396_001290"
     CDS             complement(9073..9498)
                     /locus_tag="ON396_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCR3113384.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="ON396_001291"
     CDS             complement(9565..9834)
                     /locus_tag="ON396_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCR3113385.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="ON396_001292"
     CDS             complement(9845..11440)
                     /locus_tag="ON396_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3113386.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ON396_001293"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="ON396_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR3113387.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /locus_tag="ON396_001294"
     CDS             complement(14993..15553)
                     /locus_tag="ON396_001294"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR3113388.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="ON396_001295"
     CDS             15906..16880
                     /locus_tag="ON396_001295"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCR3113389.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="ON396_001296"
     CDS             17050..18450
                     /locus_tag="ON396_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HCR3113390.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="ON396_001297"
     CDS             complement(18528..19250)
                     /locus_tag="ON396_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3113391.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="ON396_001298"
     CDS             complement(19329..19835)
                     /locus_tag="ON396_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR3113392.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
BASE COUNT         5393 a         4389 c         3535 g         6518 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttcccctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg gaatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta cacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa agaaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggttga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcat
//
LOCUS       DAJSKS010000043        19031 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJSKS010000043 DAJSKS010000000
VERSION     DAJSKS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.044"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(43..1611)
                     /locus_tag="ON396_001299"
     CDS             complement(43..1611)
                     /locus_tag="ON396_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCR3113393.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1615..1896)
                     /locus_tag="ON396_001300"
     CDS             complement(1615..1896)
                     /locus_tag="ON396_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCR3113394.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(1893..2816)
                     /locus_tag="ON396_001301"
     CDS             complement(1893..2816)
                     /locus_tag="ON396_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HCR3113395.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2813..3691)
                     /locus_tag="ON396_001302"
     CDS             complement(2813..3691)
                     /locus_tag="ON396_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCR3113396.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3678..5015)
                     /locus_tag="ON396_001303"
     CDS             complement(3678..5015)
                     /locus_tag="ON396_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCR3113397.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5018..6121)
                     /locus_tag="ON396_001304"
     CDS             complement(5018..6121)
                     /locus_tag="ON396_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR3113398.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6195..7703)
                     /locus_tag="ON396_001305"
     CDS             complement(6195..7703)
                     /locus_tag="ON396_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3113399.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7669..8361)
                     /locus_tag="ON396_001306"
     CDS             complement(7669..8361)
                     /locus_tag="ON396_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR3113400.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8383..9357)
                     /locus_tag="ON396_001307"
     CDS             complement(8383..9357)
                     /locus_tag="ON396_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3113401.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9657..10529
                     /locus_tag="ON396_001308"
     CDS             9657..10529
                     /locus_tag="ON396_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3113402.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11111..13309)
                     /locus_tag="ON396_001309"
     CDS             complement(11111..13309)
                     /locus_tag="ON396_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3113403.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13541..14533
                     /locus_tag="ON396_001310"
     CDS             13541..14533
                     /locus_tag="ON396_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3113404.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14593..16014
                     /locus_tag="ON396_001311"
     CDS             14593..16014
                     /locus_tag="ON396_001311"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR3113405.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMLPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            16007..17506
                     /locus_tag="ON396_001312"
     CDS             16007..17506
                     /locus_tag="ON396_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR3113406.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17545..18939
                     /gene="uxaC"
                     /locus_tag="ON396_001313"
     CDS             17545..18939
                     /gene="uxaC"
                     /locus_tag="ON396_001313"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR3113407.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         5714 a         3878 c         3365 g         6074 t
ORIGIN      
        1 agtgaaaata agttagtgaa cctcatttat taaagaatta aattacagat gatcttttcc
       61 atcatacttg aaaaactcaa aatcagcata acttcctctt tttttattta gatcatgcac
      121 tgctatcccg ataaaatttc cagtaaaccc tcctgagagt atacacatgt ttttttgcgg
      181 acctactttc ttccattctt cagttgatac gtctttcaaa tgaaactgtg ctgtctcttt
      241 ctttacagtg actttcaaaa aagtcttctt ttctgtgagt ggaatcgtcc aatcttctat
      301 ttgtgctact ccgttttctg attctatcat acgtaggcat tttcccttgt cctcattcca
      361 agtaacataa caataaatat aattggattc atttaagtac agtaccagtc cagccatctg
      421 attaaaatta ttaggcatgt attcaaatgc tgtctccgca tcaaaagtaa aatccgtttg
      481 ccgaatagct aacaaatgat gttcgaaggt agattgaaga gattcaccgg aaatcagacg
      541 taaaaaacct ggtctacatt ttgtatcaca ccaatcagca tttggcaaaa tgcgtctggc
      601 attccagttc ttctttagct ctccttcaaa tgtatccata aactgatgat ctttttcttg
      661 ttgtacctct tcatttgtct caatcattat ttcttccatc ggagttgtct ggctatcttt
      721 aagtcttaac caatcgtcgg acgtccaata cacttcttga atagcggttt ctcttcccaa
      781 aatagccgca ccgtttaatg gtctggtaca aagatatgtc atgtaccatc ttcctgacgg
      841 ggtttgtact aagctcccat gtcctgtaca ttgcaatgta gaagcaggtt tgtcactagc
      901 tgtcagcata ggaaaaccag gatcaagctc gtaaggacca gtaatattct ttgctcggca
      961 aactgtaact gaatgtcctc ttcctgtacc gccttctgcc gtcatcaaat aataataccc
     1021 gttacgaaaa tacaaatgtg gtgcctccgt tttcgctaat tctgtacctg caaatatttt
     1081 atagatggga ccatcaagct tcttcttctc tgcatcatat tcttgtaata cgataccgct
     1141 ggatttattc ggtgtgtcca gacgataatc ccatagttca ttgacaagcc atttttttcc
     1201 cgtttcaaga tcatggaaca aagaaggatc aaagccgtta ctatttaaat aaattggttc
     1261 ggaccaagga cctgtgatct cagatgcagt gatcaaataa ttgtggacat ctttaaacgg
     1321 acggcctgca cttttgacat ctgtgtaaac aagataaaac aatccatcag cataactgat
     1381 ctgtggcgcc catatacttc catttgtagg atttccttgc aaagatactt gagtggtcaa
     1441 aatatccgtt gtatgtgacc agttgactaa atttttcgac tcatatactc ggattcctgg
     1501 aagccattca aaagaggaga caataatgta ataccgctct ttcactctta atatattagg
     1561 atctggattg aatcctctta aaataggatt tttcactttg atagtctgca tgattcaggc
     1621 tccttttctc attcttactc gatataaact gaatgcccca gctgctaaaa aagcaagtga
     1681 aataaacaac acaacggcaa acagaccagt catgtcctgt aataatccaa atgtattttc
     1741 taaagacaaa gttgaatgat ctacagcaag cgactgataa ctttttttaa actctgacgc
     1801 agacatatta cttaaagtaa ctgctaaccc gccaaagaaa atcaaagata gactgccacc
     1861 gaccattcct agcaatagtt cccattttct tttcatttct gaaccccctg cttttctaaa
     1921 tgtatccatt caggtctttt gatcgatgtt tcataattgt tattacgttc agagaatttg
     1981 acagtctgtt catttttctt gtcatcccaa ttatcccaac cagttgggtg aacatgccca
     2041 cctatcttac acgcttcaaa taagacattt gcatacggac gccacggtct tcctaaatag
     2101 acgtttctag tatcaggttc tgcagtcaag gtacagcttt caaaacgata ccctgttttt
     2161 tgatttttcg gcgtagatgc tgctgtgatg tatccaggat ttttactggc taagctatgg
     2221 atatgacact gatgaaaaac ggcatctgcc ccaccaaata tgaaatccac cgttccttca
     2281 atccagcaat attcataggt ctgacgacaa tactcaggat tggcagaagc cggtgtacaa
     2341 aaaggagtac catctttttg caaatcaggc aacggacccg tgcataaggt atcctgataa
     2401 ccagatagtc gacagttttt taaagatacg cgatctcctg aattgaataa agcaacagcc
     2461 tgtcctacct tttctcctgg acctgctgta ttttcgatgt gaagattttc taatacgaca
     2521 ttcgatcctt ctaagaaaaa agtagcactt cggaaagtcc ctaactggct accgtcaaga
     2581 tactcctgtt ttgcaaaacg attaccggag ataactactg ttcctgctcc cacaagttta
     2641 aaattggata atctgctttc tatatattca tcatatcttc cttcttttac tatcaagacc
     2701 tttggatggc tatccggcag gcctgccaaa taatcgattc cttcttggat agtttcaaaa
     2761 tccgtcgggg tatctatagc attctttcca attatcagtt tgtctttcat cctcataaat
     2821 gctcccttat gaattgtttc caaatatttc tcatttcttg tgtttccata tgaccgcctg
     2881 ttaaagaaaa agaagaaaag ttttctggac tacccattga ttgatacttt ttgtccagcc
     2941 cttttctcag cttcagtacg ccttcgtcca agcacatcgt atcgtttttt ccaactaagc
     3001 taagacgagg gcgcggagcg attttttctt ggattgctaa cgttgtataa gctgtcaata
     3061 gatttggcac atagtaataa aacccatgat gatctaggcg gcgatgtagc aaaagtgttt
     3121 cgatgtccac ctgtgccgct agatcaatac aaactttgac acgagaatcc aatgctgtca
     3181 cccaccagct taataagcct cccatagaaa gaccaatcgt tgcgagccgt tgagtatctg
     3241 tgtcttccct tgtctccaaa taatcgatca agctgatcac atcataaata cgcattcccc
     3301 ataacgtttt tcctgttaat aaaaattgtt tgaacaactc gctttcagaa atacctccgc
     3361 gttcttcaaa tccccaagca tcaatcgacc aaacagcata tcccattcct gtcagtactt
     3421 ctgcaaaaga tggagaagct aagtaaggag caccatggat caattcactt ttcccgactg
     3481 aaaaatctcc gccgtgagaa tgcaggtaaa ggaccgtcgg aaacggtcct tctttttctg
     3541 gataagcata gagggctggt actgtttcca attcatttaa gtacaattga tagtattcaa
     3601 gcataaaacc gttttgtttt tctcggtata atcgttttcc actaggggta tgtctgctag
     3661 atggagcacc taaaaaatca ttcaactgca taagcaaagt ttttacgaac aaccagttca
     3721 tccatatttt ctgattgatt tgattggaat ttctcaatac tgatttcttt agtattttct
     3781 aatagaaatg ctggtccctc gtgattttca atcgacatct ggttaaagga aaccttaccc
     3841 gcaaagccaa tataaaatcc ttgtctgctc atttcctcta ttcctgtcat catagcagga
     3901 acaccgggaa ctgcatcttg ggccatcgag acatcgatat tgtcaaatgt gatttcttgt
     3961 atgaattgtt ccgctaatcc ataaataaaa cctgcagaag catggacatt ccttgccgtg
     4021 atattcgaaa aatggatcct tctgaatgct gatgtccgtt catctatcgg ataagccttt
     4081 ttttcccata catacggttc tttccctcgc ggaccacaga aataataaag attcagtata
     4141 aatggacaca tcacattatc catgactata ttggatactc gaatatcttc aacgatcccg
     4201 cctcgacctc gtctagattt caatcgtatt cctcgatcag tttcttggaa gatacaattt
     4261 gaaatggtaa tattacgaat actaccactc atttcacttc ctaatacgac accgccatgt
     4321 ccatggacca tcgtgcaatt tgtgatcgtg atattttcgc aagcgatcct ttcgtacgta
     4381 tcttcagtcc ctgccttgat tgcaatacag tcatctccca cgtcaatatg gcaattacta
     4441 atacgcacat ttttgcatga ttctggatca attccgtctg tatttggtga atccgctggg
     4501 ttcaagatcg tcaagttatc aaacgttgca ttactgcaaa gaatcgggtt gatcgtccaa
     4561 cttggtgact ggatcaattt aatatctttc acagtaattc gatgacagtt atggaaactc
     4621 atcaatttag gacgaggata agcgagatta tctggttcat ttctaaaagt atgccaccat
     4681 ttttttccat ttccatctaa tgtaccgaaa ccagtgacag agatgttctc tacattttga
     4741 gcatagatac aggaagcata aacttttcga tggactcctt cccaacgtga atgaacaaca
     4801 ggataatctt ttggatcgtc agagaatttc agaaccgctc ctgcgcttaa atgaagttcg
     4861 acattgcttt ttaaaaataa agctcctgtt agaaattcac ctgctggaac aactacggtc
     4921 ccgcctccgt cacttgctgc agcatcaatg gcttgttgta ttgcttcagt attcaattca
     4981 tcaattgatg cgccaaatgt caaaatatca tacatattta taatgcctcc acttcaatca
     5041 atgcttgcaa gaatgcacca acgccttttt gatcattaca aataattggt tcactgatgt
     5101 agtaagcata tgaaccatct cgctgatctg ctccgcctaa ccctgctact tgacagtttt
     5161 tattcaagtt cagccagcct tcttttgttt ccagaacaaa ttcatcaagt aatccttgat
     5221 gtgatttctt cacttgtgga agccattctt ctttgttcag cagtcccaaa cgtattcctt
     5281 tagccattgc gtaaacaaac atactactgc cggaagcttc cagataattt cctttccgtt
     5341 ttgtttctgt tgttacttga taccatgtcc cgctgttttc ttcttgatat ggaagtaatg
     5401 cagccagtgt cttttctaga atttctttca gcaaagaaca atctgtttct tcatgaagta
     5461 attcataggt atctgctgca gccatcaaat accagccaat cgatcggctc caaaaatgat
     5521 gggatagacc tgtttttgga tcagcccatg gttggacctt cttttcatcc catgcatgaa
     5581 tcagcaaatt tgtttttgga tcgaatagat gtttataact gatttcaaac tgtctcaaaa
     5641 catcttggta atctttttct tcactaaaat tctggataaa ttcagcataa aaaggagaac
     5701 ccatatataa gccatccaac caaatttgat aaggataaat ttctttatgc caaaaagctc
     5761 cttcggatgt gcgtggatgg tttgccaact gctgggaaag gagttctgct gcttttttat
     5821 atttttcttc gcctgtttct ttgtacaata aaaagaaaag ctttcctgta tttacatggt
     5881 cgatattgta ttcttcaacg ctgtagccac ggatcgttcc atcttcttgc acgaaatagt
     5941 cgatattgtc ttttatgaaa tcaaaataaa ttttttcttg ggttcgttcc caaagtaatt
     6001 ggaacccttt aagtacgacg ccatagtcat aagaccattt tcctttatac cacttttctt
     6061 catataagcg aggtgttctt tccatgatac tttttgctaa tttctttgac cagttttcca
     6121 ttatttgctc tcctttaaaa attaaaatag gatttgggac aaataccaag aagtatttgt
     6181 cccaatcgtc tatattactg tgccgctgct ttgtatgctt cctcgtactc ttgtgtgatt
     6241 cgatcgcctc cttgtgcttt ccagtcttct acagcttttt tgaatccatc ttcgtcaagc
     6301 tgtcccatga tgtattgcac cgtagcatca atgacgattt tttcaatttc tgatcctact
     6361 tcattgttcg tcggtgaatc caatggcaca gaaggatcca ttacagcaaa ttcttcattt
     6421 tctttgatca attgattaga gaattctttt tcagggtctg tatctttgaa agagaacgtc
     6481 acttcactag gacgagagct tgataacggt tgcacctctt gttgccaaag atccatatcg
     6541 ataaattcga tagaaccatc atcattcaat tcataatgcg ttccttcgat accgccagtc
     6601 atcaatttat acacatcttc ttctaaaaga tcatcaacaa attgaagtaa tttcttcaat
     6661 tgtgcttcgt cttttacttg agatttggga aatgctagca agccgccaat accatttcct
     6721 tctgaccaaa catgataacc gccgccttca ccatctgaga tcttgtttac aggcactaat
     6781 tccatatcat cttgaatacc ttgacccatc gtcttcagat tcttgatatc caccattcca
     6841 gtgtagattc cagttcttcc ttgtgcaaat tgttcttgct gatcagtctt ttgggtaact
     6901 gcaaagtctt gtgccaaata gccattctca aatagatctc ttgaaaaatt caaagctttc
     6961 atatactctt ctgtatcaaa ttctggagtg aatttgccat tatcgtctac agcccagcca
     7021 tttggtgctc caaaataaga agtgaaaagt ttaaagctgg tataacgaat atcactaggc
     7081 gcacgatcgc caaaaccaac tgtgtcgtcc acaccatttc catcaggatc gttttctgta
     7141 aattgtttag ctacttcata aagctcatcc atggtagttg gaacatctag tcctagatta
     7201 tccaaccagt cttttcgaat gactaagcca gcacgagcgt agtctttttg tatcggtacg
     7261 ccgtacaagg caccgtcgat actcgcagct tcgattctat cttcagaaat ttgacttaaa
     7321 tttttatagt cttttaaata ttctgataca tcccagaata cgcctgattt taacgcattg
     7381 cggacagaag agtttttcaa catcgcaatt tgaagtgtaa cgatatctcc tagttcatca
     7441 gaagccaatg cagttgttaa tctttcttct tttgaagcat caggaatcca gttgaatgtc
     7501 agttccgtat cggttttttc ttcgatcaag ttcacaatcg tgtctgtagg cggtgaagca
     7561 tgatgcaaaa tatttaacca agtgatttta gcttttccat cttctgtctc tgaagaactg
     7621 ttacttgaac tgctgccgca tgcggcaagt gctagaattc ctgcaaaact aagacctaat
     7681 tttacgagcg ttgctttttt cataaatttt ctccttcact gttttaataa tatttttgtt
     7741 ttcttctgtt ttttctgtat atgagttctt ccattccaaa tggcaaaata tagaatttgc
     7801 tgtccacgtg actgcggaaa agaagagact gccaccgata aaggttaaaa cacctggcat
     7861 tgtttggatg atcatcagat aaatccctac catacttaat atcaacacta atgagtaatg
     7921 aagatgtgag aatccgaata aaaatgccat tttcattttg aaaaaatatc ctttccaatc
     7981 ataatgagcc atgattggga aaatatagag tgctgagagc acatatatga acgcagcaat
     8041 atacaaagcg atcctaagat accaattgtg tatgaaagaa agatccacaa gtaaaacaat
     8101 cccgatggcg ccatatatcc agctcatcag caaggtttca cgatagtttt ctttaaaata
     8161 tgtgaagtac gttttcgtca aaggaaacgt ttcttttgag cgcagccatt ttctaatcac
     8221 tgcaaccatc gcatacgatg caggaccgaa agtaaaaact cccaaacctg cgatgactaa
     8281 accgccccac agaaaattca aataaaccaa ctgtaaaata cgatttagcc ttccgtttat
     8341 tttatctaat gactgcagca tatctttccc tctctttcta tcttagtaaa cgccgtcttc
     8401 tcctagcttt ttggcaatct tattagctaa aattaccata aacaatccta cgaccccttt
     8461 aaacaatccc attgctgtac tgaaacttaa ctgtccattc ttcaatccag ctgtataaat
     8521 ataggtgtcg aaaatttctg caacgcttcg attcaatgaa ttcaataaca agtacatatg
     8581 ctcgaaccct aagtctaatg tctgaccgat ttttagaatc agcaaggtaa tgattactgg
     8641 acgaattgcc ggcaatgtaa catgccatgt tttgcgtaag cgtcctgctc catccatttc
     8701 tgcagcttca tacaactgtg tatcgactgc agttatcgct gaaaggtaga tgatcgtaga
     8761 ccagcccaac tctttccaga tcacttgtcc gatatatact gtgcgtgtcc attcagggga
     8821 tgttagaaaa ctgatttttt caaatcccat tgttgcaagg aattcattca atacccctcc
     8881 gtcaacattc aaaaatacat aagtaatcga aacaatgatt acccaagaca tgaagtgagg
     8941 gatgtaaatg atcgtttgaa tcccgttttt caatcgtttg ttcgttactt catttaatag
     9001 taaagacaaa atgattggca tcggaaaaaa cacgaagata tttaatccga acaagatcaa
     9061 cgtatttctt aataatgtaa agaatgtcgg ttctgtaaac aaacgaatga aatgtttgaa
     9121 accgacccaa ggacttccca ttattcctaa aaatggttga taatcttgaa aagcaataac
     9181 gagtcccccc ataggaagat acctaaaaat caagaaataa aggactcccg gcagaatcat
     9241 cacatataaa aatttattcg ttttgattcc tgcaattatt ttttttcgtc ttctgagctt
     9301 ttcttctttt ttcgatagcg tcagttgaaa tggttcttgt ttctttacac tttccataag
     9361 cccctcctta gttttgtgaa tgtttgctgc ttatgacttc atgatagata actatctgaa
     9421 ttacgtatat aatcattttt atcgtaagta aaaacgctta catagcaatg atttttgtgc
     9481 tatacaaaac gataaagtgt attttttcac aaataaacta cgcatagcaa accttctatt
     9541 tttttctatc tgacacttag cataggaaat gattatatac gtaatctttc accgatgata
     9601 aattgaacat atagaaacag aaacatttaa tttattttga ggaggagcct atctatatga
     9661 aaaagaaagt ctctatagga gaaagaagtt ttacactttt caacaatacg tttctaattt
     9721 tgctagcttt gatctgtatc gttccttttt taaatatcat cgctacatct tttgcttcta
     9781 cacaagaagt cgtcgcgaaa aagtttatcc tttttcctac taccttttct ttagacgcgt
     9841 atcgctacat tctttctacg ccgaccattt ttagagcact tgctgtttcg attggtgtta
     9901 caggtgtcgg aacgatcgtc agtatgtgtg caacttcact gatggcctac ggtctttcca
     9961 gaaagtattt gtttggtcga ggtttcgtca actttcttgt tgtattttct atgttgttca
    10021 gcggggggat gattccaacg ttcttagttg ttcgttctct agggctggtc aattcttact
    10081 gggccatgat cctgcctgtg gctgttaatg caatgaatat gatcattatg agaaactttt
    10141 tccaagcctt gccggatagt ttggaagaat cagcaaaaat ggatggttgc acagattttg
    10201 gtgtattttt caaaattatg ctgccattag ctttaccgtc tattgctact atctcgcttt
    10261 tttatgctgt tacttactgg aacacttata tgactgctat tttgtatatc aacgattcat
    10321 ctaaatggcc gatacagatt cttttacgcc aaatcgtcat tgtatcaagt gggatgcaag
    10381 cagaaagtag tgccgttgat gtgattccac cagcacaaac agttaaaatg gctgttatcg
    10441 ttattgcaac agtgcctatg ctgattgttt atccttttgt tcagaaatat tttgtcaaag
    10501 gagcactcgt aggttctgtc aaagggtgat tatgtactga acgcaaaatg gtagttgtgt
    10561 cagaagtggt cagcttcaag aaataagaag gcatttacga aaattgcttc ttaaattttt
    10621 gtgaaatttc ggcttatttt tcgaagaatc tactcctgga acaccgtgta tcagaggttg
    10681 tgaaagagac gttcagcctt gagcgatgat aaaaactctc acaaacattg cgacgagtaa
    10741 cccacaggag caataagaag cgattttgaa aaatagcttc ccatatttca caaaattatg
    10801 ccttattgtc gatagcctgt ctcttgaacg ccatttattc gtaaaaaaga actgtgacaa
    10861 gacttttgtc acagttcttt ttgcgtgctt agccttgaaa tttaactatc cataaataca
    10921 aatactaggg ttattctaac ttcttttatt gcttgaatca gtccacaaga aaaacagcct
    10981 cttgattaaa cggcgtttat cttgttgatt ctgcggcaat aaaatcagcc agcagcaaaa
    11041 catgaagtgc tgttttttgc tgtcctcttt tattgcttga atcagatcac aagaaaaaca
    11101 gcctcttgat tcacaaatat ttttttcgat attctcccgg cgtaatcgat tccttctttt
    11161 taaagaaacg aataaaattt tgcggatttc gatattgcaa tctttctgca atctctttta
    11221 cggagaggtt agtctctttc aaccattttt ttgctacctc taaacgatga ttttgaacaa
    11281 aatcaccaaa tttttctcct gtttcctttt taaatacatt acttaaatag ttagggttat
    11341 aatgcaacct atcagcgatc acatctaaag acagatcacg atcatattct gtgtagacga
    11401 tatgcataat attctccgac aaatttttaa attcatgatc agtgattgct tgagagcatt
    11461 ctgtgattgg cagaactaat tgttctaaaa caagtttttc tagttctttg ggatgataat
    11521 tgttcagtgc ttttacataa atttgtttca tgttatcaaa gttttttgaa tctactccca
    11581 ataactgtcc taattgaacg agttcattga tcaagcggac cgaaactact tcccgactca
    11641 aaggattttt attttgtgca aacaatgcat caatcaattc atgtactaaa ttcttcactt
    11701 cctgatttcc agaacgaatg gcttcaaaca atcgattttg ttcttctatc ggatatctaa
    11761 taagtgtttt ttcatgagca gcaggaacga tttcctgata aaaaataatt gaacttggac
    11821 cagtatttac acgataatac agggcttctt ttgcccgatc aaccgattgc ttgctttcat
    11881 tcagcgtttc gaaccggtta ctgatgccta tactgatagt taaattcaag tattctttta
    11941 cacgtttttg aatgagtcgg caatactcca tgactttttt gcttgcttct gcatcatctt
    12001 tatccattcg ataaatagtg gcttgcattt cttcattcaa aacgatcggc agcattcgat
    12061 tttcctttgg cacaatttcc gtgatcatgt tgttgatcgc taaaagtagc aaatcacgct
    12121 ccccaccgtg actatcttct aacagatcga tttgaaccaa agctgtataa tagcagtcat
    12181 tttgagaact ataaccaaat tgctgcaacc gctgatctag ttctcgcctt tctactcgat
    12241 ttctaaataa actaagtaca aatagcgttt ccagctgtgg cttttgggta actaagtgtg
    12301 cacttaacgc ttctttttct cctatgatcc gactgactga ttgagcgacc aaatctagtt
    12361 ctttcccact gaatcctttt gatttaagat tcagacgttc ctgtatttga gaaatcggac
    12421 gggcaaatct gtccgaaaat aagtaggaaa aaagaccaat gagtcctacc agtgttaaag
    12481 acactgcaac tagcccgatt ctcagattgc ggatcattgc gccaattgca ctagagtcaa
    12541 cttctaatct gtaaatccaa ttattataat ctgatttcag tactatatac gttttatctt
    12601 tttctcttat cactttcagc tgattgtaat ctagatccac gtccgttaat acagttggtt
    12661 gctctttatt ttttactgtc tgagtagtaa aaaggttgcc aacttcgttt tcaatagaaa
    12721 gtatgtcttc tccactttca ccaacaactt gttctattgt atgtttctct atcgtagcaa
    12781 ttcccaatgc atatttttct ttttcaaaca ttggcaaaga aatcaccatt tcgattcctt
    12841 tgtttttctt ttgccaaaat aaattatttt tgttatttat gtacttctgt tgatactttt
    12901 caacttcctc ttcggtcagt tgactcaacg atccattgat tactccccat ttaccagcca
    12961 aactgactaa atcataatta ttatcatcca taacaatcgt ttctataaaa tttagttctt
    13021 tcctgatttc agaataagtg gagtaatctg tatatgtcag cggatgcttg aatatgtccg
    13081 agaaattttt ggtcgaacca tacgtagaaa acgaatattc aatcgttcta attttccatt
    13141 caacgttagt tttaacttgc ctgagcaaat tctttttgtc tgttaaataa ctcgattcga
    13201 tcgattcata ggtattctgg taaatgaata aactaaatcc tatgacaagt acggctccta
    13261 ataaaaacat cggtaaaaat attttataaa atactgtact tttcttcatt ttcttcctct
    13321 cctcctttta aaataaaagg ctttcatttt tctgtaaaat tgatactcat ctaattaaat
    13381 gagcattccc cgctttcaag aatagcataa ttaaaaataa aaatccaagt aatttttaac
    13441 gtgtgcaatc ttttcatatc aaagatctat tgacttatcg tagttatcgt aatatgatga
    13501 atgcgttaac aaaaagtgaa aaagtaagga aaatgatgga atggtaacga tcaaagatat
    13561 cgctaaagca gctggcgttt ctcatacgac agtttctcgt gctttgaata acagcagttt
    13621 aataaaaaat gaaacaaaac aaaaaatacg acaactagct aaggaaatga attattcgcc
    13681 aaactatagt gcaaaaggtt tagtgaatca aaaaaaatat ctaatcggac tatttttctc
    13741 aagtttgcat aaaggaactt catcaaattt cctagcagat tccattgcag gcatccattc
    13801 agtattagac acaaattaca gtttatctgt tgaaagtatc gatgttgttg atttaacaga
    13861 aatcaacctg caacgctacg atggactgat cgttatgagt caaagtgatg atgatcagcc
    13921 tttcatcgac tatctcaaag aaaaagaatt tcctttcgtt gttgtcaatc gtcacatcaa
    13981 tgatccagcg gttatcaacg tcactgccga tgatgcggcc ggtgttatgc aggcagtaga
    14041 ttacggtatt ggatgcggac atacgaagat cgcttatatt ggcggaaaga aaaattttcg
    14101 ttcgacaaaa gaacgggaaa aaggggtatt gaaaagtcta aaaaaagcga gcatccctgt
    14161 caatctagcc tattttttaa caggagatta cagcttagaa agcggcctcc aaaacatgga
    14221 atacctatta tcactttctg acctgcctac tctggttttt tgcggcaacg acgacatagc
    14281 gattggtgca ttgagagctg tacatttagc tggcctccgt gttccccaga acatttctct
    14341 gatcgggttc gatgattctc cagtcgtctc ctatctcaat ccgccgctta ccaccgtacg
    14401 taagcctttg aaagaaatgt gtcaagaagg aacaaagctt ttactagatt tgatcgaagg
    14461 caggacgatc gaacaatcta aaattgagct ctccaccttg ttgatccttc gtgagtcgat
    14521 caattacttg taaactacaa gtaattttat ataaaaatgt taacgtgtgc ataacacaga
    14581 aaggaagttt atatgaaaaa actgacaaaa gaattagtga aaaaaaacga agcacctgta
    14641 aaagttcttc agtttggtga aggcaatttt atgcgtggat ttattgattg gcagattcag
    14701 cagctgaata accaacaatt gtttaaaggt aatgttgcaa tcattcaacc tttagaacaa
    14761 ggtcttggaa atatgatgaa ccagcaagat aatttatata cggtcatttt acaaggactc
    14821 agcaatggcg aaatcgttga tacttccgag attatcaccg tagtagaaca aatgatcaat
    14881 ccttatgatg aatgggatgc ttatctatct ctagcagaaa atgacgactt ggcctatatc
    14941 gtctccaata caacagaggc aggaatccaa ttcgtggaga ctgataattt aacagatact
    15001 cccccacaga gcttcccggg caaactgaca gcctttttat accgacgttt caaattaaac
    15061 aaagctggct ttaccatcat tccttgcgaa ctgattgaca gaaacggcga acgtctaaaa
    15121 gaaatcattt tacgctacgc agaaaaatgg gatttagaac ctgcttttgt tgagtggtta
    15181 gaaaaagaaa atgttttctg ttgtagttta gttgatcgca tcgtcccagg ttatcctcgc
    15241 gaagatgcag gtgttttcaa tcaacggcat aactatgaag atcaattgat ggtcaaagca
    15301 gaaccattca tgctttgggt gatcgaaggg cctaaagaat tggagaatca actaccattc
    15361 aaacaggctg gcttgaatgt catcatcaca gatgatatga ctccttatcg tgaacgtaag
    15421 gtgcatttgc ttaatggacc gcatacagcg atgctccctc ttgctttgtt ggcggaattg
    15481 gaaactgtag aagaagtaat gaaagatcct ttattctccg catatgttga tcagttattc
    15541 aatctagaat tgattcctat gctttcttta cctaaagatg aattagccat ttatgctgac
    15601 caaatcaaag aacgtttctt gaatcctttt gcccatcaca aattagaagc gatttcatta
    15661 aatagtgttt caaagttcag cacccgtctg cttccagtgt tcaagaagta tatcgaagaa
    15721 caaaatcagg taccaccgtt aatcactgtt tcactagctg cgctacttct tatgtatcga
    15781 ggcgatcaag taaagccaca tgatgatgaa aagacgataa gcgaatttac tgatgcttgg
    15841 tctgataatg gaacagctat tcctcgatta ttgcaaaacg ctgcgctttg gggagaagat
    15901 ttgtctcaaa taccgaatgt gacagataca gttcaagaaa tagctgcaaa gatcgatcaa
    15961 aaaggtgtcc ggtcagtatt acaagaaatg aaaggaacga tggtccatgc ttgaaaccct
    16021 gcaaccaaca atgaaattaa atcgacgaga cagtgtggtc gtcgcattaa cccctattcc
    16081 aaaacaaaca caactgacga ttgatcagca aacgatcacc actcttgaag atatcccgca
    16141 aggtcataag attgcattgg gtgatttgaa agctggagac aacgtgatca aatatggcta
    16201 tccaatcggt catgtgacga cagacgtaac tgccggtcaa tggctgcaca ctcataatgt
    16261 aaaaaccaat ctttctggtg aattggatta tcgatatgaa aaagatgtcc atccaagtca
    16321 ttaccttttt gagaaccgaa catttcaagg ttacttgcga aaaaatggaa aagtagggat
    16381 tcgtaatgat ttgtttttag tacctaccgt tgggtgtgtg aatggcattg ctgaactgat
    16441 cgtcaaacag ttcaaggaaa aacatccaga tctgggacag tttgaccata ttacgatttt
    16501 aaagcatcct tatggctgtt cgcagcttgg aaaagatcat caaaatactc gagaaatttt
    16561 agcggacgca gtaaatcatc ccaatgccgg aggagtttta gtatttggat taggttgtga
    16621 gaacaataca gttcctgaat tcaaaaaaat tttaggtgaa tatgatgaag aacgagtgaa
    16681 atttctagtg gctcaagatg tttacgatga gatcgaacaa ggtgtggctc tattagaaga
    16741 gcttttaaca gcggcggaaa atgaccagcg catatcagtc ccgctctcta agttgaatgt
    16801 cggtttgaaa tgcggcggtt ctgatggtct atcgggaata actgctaatc ctttacttgg
    16861 tgcattttct gactacttga ttgctcaagg aggcagcacg atcttaacag aagtgccaga
    16921 aatgtttggc gcagaacaag tattgatggc acgagcggaa aatgaagaag tttttgactc
    16981 aatcgttgac ttgatcaatg acttcaaaca gtatttcttg tcttacgggg aaccagttta
    17041 cgaaaaccca tctcctggta ataaagcagg tgggatcact acattagaag ataagtcttt
    17101 aggctgcacg caaaaatcag gaagctctcc tgtcgttgat gtgctacaat atggcgaaaa
    17161 aatcagaaaa ccagggctta gtttattgca agctcccggg aatgatcttg tagctgcctc
    17221 tgctcttgct tcatccgatt gccagttggt cttgtttacc acaggaagag ggactccgtt
    17281 tggctcatat gtcccaacgg tcaaggtatc tacgaataca actctttttg aacgcaaagg
    17341 tcattggatg gattttaatg caggcgtctt attagaacaa ccaatggaag taatcttaga
    17401 atcgtttgtt caaaaaatca tcgctgtagc tagtggagaa gaaacgaaaa atgaacaaaa
    17461 tgacgttcga gaaattgcca tttttaaaaa cggagtaaca ctttgatttt cataaaacag
    17521 tataaaaaga aagaaggaag atttatgttt ttatctgatg attttttatt aaaagacgat
    17581 tgggcgaaaa agctttacca ttcctacgca aaaaacatgc ccattattga ttatcactgt
    17641 cacttagatc caaaagaaat ttacgaaaac aacaatttca gcaatctgac agaagcttgg
    17701 ttaggaggcg atcattacaa atggcgcttg atgcgagctt gcggcgtgcc tgaagaaaaa
    17761 atcacaggaa atgcttctga ttttgaaaaa tttttagctt ggtgtcaaac cgtaccaaaa
    17821 attatcggaa atccgcttta cagttggtca catcttgaat tgaagcgttt cttcggtatt
    17881 gacttgttga taaatgaaga aaatgcagag aaaatttgga cacaagcaaa tgaaattttg
    17941 gcagctcctg attttcgccg tcgggaaatc gcaaaaaaat cgaatgtaga agtgatttgt
    18001 acaacagatg atcccgcaga cgacttgcac tatcatcagt tgcttgctca tgaagaacca
    18061 gatttgaagg ttctaccttc attccgtcca gataaagcac tcaatattga aaaagaagga
    18121 tttaatgcat gggtcacact actggaagaa gcagctgacc aaacgatcac cacctatcaa
    18181 gaactgatag aagtcttagg gcagcggata gaatacttcc atcaacatgg atgtcgtgtt
    18241 tccgaccacg ctttagacat cttacgctat aaagaagcgg atgaaacagt attagaagag
    18301 atttttcaaa aagcacgagc taacaaacta ttatcttcag atgaaatcga tgcttatctt
    18361 acagaaacac tgatacattt gacccacttc tatcattctc ataattggac gatgcagctg
    18421 cacatccatg cttatcgtaa ctgtaataca caaatgttgg aaaaattagg gcccgatacc
    18481 ggctatgatg gaatgaatga tctttctttg actattcctc tgcaaaaatt attgaaccgt
    18541 gcagaagaaa cggatcagtt gccaaagacg atcctttatt cattgaatcc gaatgactat
    18601 cctgcgattt tagctttgat gggctgtttc caaaaagaac agaatggaaa actgcagtta
    18661 ggttctggat ggtggtacaa cgatactcga gctggtatgc gcgatcaatt gacacaattt
    18721 gctgatggta gtgctttggg gaattttgtc ggcatgctga cagattcaag aagtttcctg
    18781 tcctacacta gacatgaata tttccgtcgt gttctttgtg aatttttagg cgaaatggtc
    18841 atgcgaggag aagcacctga agatgaacaa cttttaggaa gtctagttca agcgattagt
    18901 tatacaaatg ctaaaaatta ctttggcttt tttgcctaat tgattaatag taacagtaac
    18961 gaatcaaaaa caggatagtg aaataacgga atccgtttat ccacgtttta gagaagaaga
    19021 cggaccaaat t
//
LOCUS       DAJSKS010000044        18856 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJSKS010000044 DAJSKS010000000
VERSION     DAJSKS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.045"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(111..1409)
                     /gene="eno"
                     /locus_tag="ON396_001314"
     CDS             complement(111..1409)
                     /gene="eno"
                     /locus_tag="ON396_001314"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR3113408.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1525..2280)
                     /locus_tag="ON396_001315"
     CDS             complement(1525..2280)
                     /locus_tag="ON396_001315"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR3113409.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2384..3574)
                     /locus_tag="ON396_001316"
     CDS             complement(2384..3574)
                     /locus_tag="ON396_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR3113410.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3684..4685)
                     /gene="gap"
                     /locus_tag="ON396_001317"
     CDS             complement(3684..4685)
                     /gene="gap"
                     /locus_tag="ON396_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR3113411.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4726..5817)
                     /locus_tag="ON396_001318"
     CDS             complement(4726..5817)
                     /locus_tag="ON396_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HCR3113412.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(5966..7300)
                     /gene="rpoN"
                     /locus_tag="ON396_001319"
     CDS             complement(5966..7300)
                     /gene="rpoN"
                     /locus_tag="ON396_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR3113413.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            7360..7431
                     /locus_tag="ON396_001320"
     tRNA            7360..7431
                     /locus_tag="ON396_001320"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7393..7395,aa:Arg,seq:ccg)
     gene            complement(7623..8507)
                     /locus_tag="ON396_001321"
     CDS             complement(7623..8507)
                     /locus_tag="ON396_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR3113414.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(8594..9493)
                     /locus_tag="ON396_001322"
     CDS             complement(8594..9493)
                     /locus_tag="ON396_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HCR3113415.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(9510..10016)
                     /locus_tag="ON396_001323"
     CDS             complement(9510..10016)
                     /locus_tag="ON396_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCR3113416.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(10205..10825)
                     /locus_tag="ON396_001324"
     CDS             complement(10205..10825)
                     /locus_tag="ON396_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCR3113417.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(10901..11338)
                     /locus_tag="ON396_001325"
     CDS             complement(10901..11338)
                     /locus_tag="ON396_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCR3113418.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            11501..12331
                     /locus_tag="ON396_001326"
     CDS             11501..12331
                     /locus_tag="ON396_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCR3113419.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            12315..13682
                     /locus_tag="ON396_001327"
     CDS             12315..13682
                     /locus_tag="ON396_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3113420.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            13701..14510
                     /gene="yidA"
                     /locus_tag="ON396_001328"
     CDS             13701..14510
                     /gene="yidA"
                     /locus_tag="ON396_001328"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR3113421.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(14553..14996)
                     /locus_tag="ON396_001329"
     CDS             complement(14553..14996)
                     /locus_tag="ON396_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCR3113422.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            15130..15501
                     /locus_tag="ON396_001330"
     CDS             15130..15501
                     /locus_tag="ON396_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3113423.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(15670..16452)
                     /locus_tag="ON396_001331"
     CDS             complement(15670..16452)
                     /locus_tag="ON396_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HCR3113424.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16449..17420)
                     /locus_tag="ON396_001332"
     CDS             complement(16449..17420)
                     /locus_tag="ON396_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HCR3113425.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            17512..>18856
                     /locus_tag="ON396_001333"
     CDS             17512..>18856
                     /locus_tag="ON396_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR3113426.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEIL"
BASE COUNT         5305 a         3885 c         3360 g         6306 t
ORIGIN      
        1 aaactctcta cctgctgata tttcagcaag tagagagttc gattacttaa ctaagtgatc
       61 atactcgact atctttcaat agtcgatcat gtatcttcat tagaaaagaa ttatttgttt
      121 tttaagttgt agaaagattt caaacctttg tattctgcaa cttcaccaag ttggtcttcg
      181 atacgtagta attggttgta tttagcaata cggtcagtac gtgatagaga accagttttg
      241 atttggccag catttgttgc aacagcgata tcagagattg ttgaatcttc tgtttcacca
      301 gaacggtgag atacaactgc tgtgtagcca gcttctttag ccatttcgat agcttcgaaa
      361 gtttctgtta atgtaccgat ttggttcact ttgataagga ttgagttagc aattcctttt
      421 tcgatacctt cagataattt ttgagtgttt gttacgaaca agtcgtcacc aactaattga
      481 actttgtcgc ctaatacgtc agttaatttc ttgaatccgt cccagtcgtt ttcgtctaat
      541 ccatcttcga ttgagatgat tggatattta gaaactaatt cttcgtagaa tttgatcatt
      601 tcgtcagttg ttttttcgcc ttcgcctgag tcagctagta cgtaaacacc tttttctttg
      661 tcgtagaatt ctgaagaagc agcatccata gcaagaacaa cgtctttacc aggtacatag
      721 ccagcttttt cgattgcttc gatgattact tcaaaacctt cttcgtttga accaaggttt
      781 ggagcaaatc caccttcgtc accaacagaa gttgctaatc cgcgagcttt caagattgaa
      841 gctaatgcgt ggaatacttc agcacccata cgtagagctt ctttgaatgt tggagcgcca
      901 acaggcatga tcatgaattc ttggaagtcg attgagttat cagcatgtga tccgccgttg
      961 atgatgttca tcattggtgt tggcaatact tttgtgttga atccgcctag gtagtggtat
     1021 aaaggtactt ctaggtagtc agcagctgca cgtgcaacag cgatagaaac acctaaaata
     1081 gcgttcgcac ctaatttacc tttgttagga gttccgtcta aagcgatcat agctttgtcg
     1141 atagccattt ggtcacgaac atcgtagcca atgattgctt cagcaatgat gttgtttacg
     1201 ttgtcgacag ctttagttac acctttaccg ccataacgag ctttgtctcc gtcacgtaat
     1261 tcaactgctt cgtattcacc agttgaagca cctgaaggaa ccattccgcg gccaaaagct
     1321 ccgctttctg tgtatacttc tacttcgatt gttgggttac cacgtgagtc taagatctcg
     1381 cgagcgtaaa catcagtaat aattgacatg ttttgtctct cctttgagtt tgttttttcc
     1441 taagggattt ccctcatata cgattttagt taattttgct cattagtgca aacatttctt
     1501 gcatttttcg caaattttca acaattattt tacagcgtct aacaatgaca aaaatgaatc
     1561 tgcttgcaag cttgcgccac cgactaaagc accgtcaaca ttttcttttg ccatgtattc
     1621 agcaatattt tctggtttta cagaaccgcc gtattggata cgaactgctt ctgatacttc
     1681 ttttccataa agtttttcaa ctgtttgacg tacaactcca caaatttcgt cagcgatttg
     1741 tgcgtcagct gatttaccag taccgattgc ccagattggt tcataagcaa taaccatgtt
     1801 tgacacttgt tcagctgaaa gatcagctaa accagctgta atttgtcctt cgatccattc
     1861 agcagttttg cctgcttcat aggtttctaa agattcgccg cagcatagga ttggtgtcat
     1921 accatttgca aagatagctt ttgctttttt attgatgtct tcgtctgttt catggaaata
     1981 ttcacgacgt tctgaatgtc cgatgatcac ataatgaaca cctagatcag caagagctgc
     2041 tggtgaagtt tcaccagtga acgcacctga gttttcccag tagcagtttt gtgctgagat
     2101 ttttagttct gtaccttcag ctgatgtcat taattcttgt aagaataatg caggagatcc
     2161 gatgactgaa tcaactttgt catttgaagg gattttgttt tttactgctt cagcaaattc
     2221 ttttgcttct gatgcagttt tgttcatttt ccagttacct gcgataatag gtttacgcat
     2281 tgaggaacac actccttaat ttattgaaaa atggctaggg agtcatgaaa actcccttca
     2341 ccttttgatc gattactcgt cagaattgag caaacttggt cgtttattta tcgttgattg
     2401 ctgctagtcc tggtaattct ttaccttcaa gcaattccaa gcttgcgcca ccacctgttg
     2461 agatgtgtgt gaatttgtca gcaaatccta attgttctgc tgctgcagct gagtctccgc
     2521 caccgatgat tgttgttgcg ccttctaagt tagcgattgc ttcacaaaca ccgattgtac
     2581 ctttagcaaa gttactcatt tcaaatacac ccataggtcc gttccaaaca actgtttttg
     2641 caccttgtaa tgtatcagca aataatttta ctgatgctgg gccgatgtct aaagccatca
     2701 aaccatcagg aatgtctcct tcgattactt gtgtttcaac atcatttgaa aattctggtg
     2761 cacagacatt gtcgattggc aagacaagtt tgtcgcctgc tttttcgatt aattcttttg
     2821 ctagttccac tttatcagct tctactagag agttaccgat cttgattcct tttgcttcgt
     2881 agaatgtgta agtcattccg ccaccgatca agattttatc tgctttagga atcaagtttt
     2941 cgattacgcc gattttatca gaaacttttg cgccaccaag gattgcaatc attggtcgtt
     3001 ttggttcttc tactgcttca ccgatgaact tgatttcttt ttccatcaag aatccagcaa
     3061 ctgttgggat accagttgaa gcgataccta cgttagaagc atgtgcacgg tgagctgtac
     3121 cgaaagcatc gttaacaaat acatcgccta atgaagccca gtatttacct agttctgtat
     3181 catttttgct ttctttttta ccgtcaatat cttcaaatcg tgtattttcg aatacgacaa
     3241 cgtcgccgtc attcatgttg cggattgctt cttctaattg ttctccgcga gtttcaggta
     3301 cgaatgttac ttctttacct aataattcgc ctagacgttt tgcaactgga gctaaagatt
     3361 ttccttcttt atcttcttct gtttttacac gtccaaggtg agagaaaagg attgcttttc
     3421 cgccttgttc tagaacgtag ttgattgttg gcaatgctgc tttgatacga gtgtcgtcag
     3481 tgatcacgcc gtctttcaaa ggaacgttga agtcaacacg gacaagaact tttttgtctt
     3541 ttaaatcgat atctttcact gttttcttag ccattttatt tcctcctgaa taaacttatt
     3601 atcaaaaaaa gcggaagaag cgcgattgct tccccgcttt taaatgtaca tgtctataat
     3661 ttacacttgt acgtttgtct atcttataag ttagcgaagt attctaaagt acgtactaat
     3721 tgtgcagtgt atgacatttc gttgtcgtac caagcaacag ttttaactaa ttgtttgtcg
     3781 ccaactgtca ttactttagt ttgtgtagca tcaaataatg aaccgtaagt cataccaacg
     3841 atatctgaag aaacgatttc gtctgtgttg tatccgtaag attcgtttga agcttctttc
     3901 attgctgcat tgatttcgtc aacagttact tctttttcaa gaactgttac taattctgtt
     3961 aatgaacctg ttggtacagg aacacgttga gcagctccgt ctaatttacc gttcaattca
     4021 ggaattacta aaccgatagc tttagcagca cctgttgagt taggaacgat gtttgcagca
     4081 gcagcgcgtg cacggcggaa gtcaccttta ggatgaggtc cgtctagagt catttggtca
     4141 cctgtgtaag cgtggatagt tgtcattaat ccttcaacaa caccaaattt gtcgttcaat
     4201 gttttagcca taggagctaa acagttagta gtacatgaag cacctgagat aactgtttct
     4261 ttaccagtta atgtttcgtg gtttgtgtta taaacgattg ttggaacatc gtttccgcca
     4321 ggagcagaga taacaacgcg tttagcacca gctgttaaat gtttttcagc tgctgattta
     4381 gaagtgaaga aaccagtaca ttctagaacg atatctacgc ctagttcgcc ccatggtaat
     4441 tcttctgggt tgcggttagc taatacttta acttcttttc cgtttacgtt gaatgaacct
     4501 tcatgaactt caactgttcc gttgaaacgt ccttgagttg tgtcgtattt taacaagtgt
     4561 gccaacattt tagcatctgt caagtcgttg attgctacta cttcgattcc atctacatct
     4621 tggatacgac ggaatgctag acgtccaata cgtccaaaac cattaatacc tactttaact
     4681 gtcatttaag atttcctcct tatgaaaatc aaaaaaatat ttattttaaa gggttacccc
     4741 ttttaaaatc tcatttgcag cagcttcatc agtgatgagc cacgtttgtt ttggtgcatt
     4801 tttcatataa gctcgaattg ctttagcttt tgattttccg cccgcgattg ccatgatgat
     4861 cgggactttt tccaaatctt gcaattctat gccgattcta gggaccttat aaacgacttc
     4921 acctttttca ttgaaaaagt agccaaaaga ttccgcaaca gcatttgctt gtttcagcat
     4981 gactaattct ttttcagtca tcttacgacg tgctgccatg tgtaatgcac gcccaatact
     5041 atgaataaca caattcgctt cactaatcaa gtttaagact tcttggatag atggctcatt
     5101 gagtaaagaa ttgtaagttg ccagacttaa ttgttccgga acataaagtg cacggtggtt
     5161 accgtttgct ttagcagcca ttcttgcact gacagaattt gcctgtacag ttatcgcttc
     5221 accaatcccg cctcttgcag ggacaaacag gttatgccgt ttcttatttt ccagattaga
     5281 caattgttcg gctaccacag ccatagtagt tcctcccatc acagcgatga tattctcgcc
     5341 ttctggaagt agtcggtcta gggcttcgct cagcgcttcg ccgaatgcat cagccacttt
     5401 ggtctgctct tcgctgtttc ctgaaacaac gatacagcgt tggttaccaa aatattctgc
     5461 caactgttgt tcggtttgat gcgtacctaa aagctgatcc atgaagtttt caaggctttg
     5521 ataaacttct tcgcccttag ttgtcagcgt cataccgctt tttgaggtgt cgatcaaatt
     5581 cagatttttc aaaagatctg tttctgttcg caatactcgt tctgtgatcc ccatactttc
     5641 cgataaactt cgacgtccaa tcggctgcat ccaatagata tttcgtaaaa ttttatagcg
     5701 ttcttgcaac acttcgataa tatctggtgc aacagattca atcattttta tttcatccag
     5761 catagcgatc tccttagttg gacactttac gtccaatgta gacttaaaac gacccatata
     5821 cgataaaaaa agaaattaac tattcgaatt tcacatcaat aaagggaaat ctcaacctca
     5881 cgactgtaag tctaacagtt ttgctctcgt ttttcaacta aaaagactag aaaaagtgaa
     5941 atactttttc tagtcttttg actgattagt tatcataacg ttttcttttt gatgaactcg
     6001 ggattcctaa taaatcacga tattttgcta ctgttcgtct tgagatcgct atctcatctt
     6061 ttttcagcaa ttcaaccaat ttctggtcag acaacggctt gtttttgtct tcttgatcga
     6121 ctaattcctg cagctttttc ttcgctgtat ctgctgaaag gtcttctgtc tgttctgtac
     6181 tattcgtagg aattcgtgta gtgaaaaatt tttttaattc aaatacaccg aagtctgttt
     6241 ccaaatattt cccgttgact gcacgactga ccgtggattc atgcacatct atctcagttg
     6301 caacttcctt taacgtaaga ggcttgattg gacgttcctt gtcgataaag aaagcttttt
     6361 gccgggaaac aatgacttga gcaacccgca aaattgtatc cccacgttgt aggatcgttt
     6421 tcttcaacca ttcgaattcc tgtttccgtt cttttaaata attttcagtt tctttatctg
     6481 cttgttgtga catttgtttg aagtaactct cttgaaagcg tatatttggc tgattttttc
     6541 ggtttgagat aacttgtaca tttttatcgt cgtccactaa tacccggaca tcaggaataa
     6601 tgtacaattc gctgctacgg tcgaaaatcc ggccagggct tggactcaac gtttgtaaat
     6661 agtcgaatat ctgctgaaca gcatgtagat caacagaaaa acgttgtgcg atttctttcc
     6721 attttcgctc caccagttcc tcaaaacatt cttccaacac gatataagct aattccggcg
     6781 cataatcatc tcgttctgtt tgaagcatga gacattcttg caaactacgt gcacctactc
     6841 cagctggttc taattgctga atcagagtca acgcatcgag catttggata ggcgtagctc
     6901 caatctcttt tccggcttct tctaaactaa tcgtcaaaaa tccgttcaaa tcgatgtatt
     6961 ctacaagata aagaacgatt ttcctcaaaa aagtatctcg gtaattcaaa tggacttgtt
     7021 cgatcaatga atcaaataaa gacccttttg tatcaggaat ctgatttagg taatttgtct
     7081 cttctccttc ataagaagat gaagaagctt ttatgtaatc tggctgccat tccggctcaa
     7141 caacttccac caatggattt tccattgctt gattctcaac gaatgccaac agctcctcac
     7201 tgttatattg taaaacttgg atggactgtt gcaactgttg cgtcatggct agtttctgtg
     7261 tctgctgctg tttttgtgaa aaattttgct caaatttcat ttttttgtcc aatccccttg
     7321 taatttttta tctgtttagt tacaattatc ccagtgcttg cgtccttagt gtagtggata
     7381 tcacgtaaga ttccggttct tgagacgggg gttcgattcc ctcaggacgc gttctactgc
     7441 tattttagca taatttacga atagttgctt ctttattttt aagtgaaaac tgtccgccaa
     7501 agaaaattgg tggacagttt tttgttgtac ctgtatagat attgttaagc ctttgcaagt
     7561 aaggtagtta tgtgttcaac tacttattaa aatgtgttat ttattaaaga tacttgtctc
     7621 acttactgaa ctggccgatg atcgtttctg ccataatagt tctcgacagc cgttggatgc
     7681 cctaataaat ccccaactgt cgtgatgcga tacccttgct ctttcagata atcgatcact
     7741 tgaggaacgg cacgaatcgt ttcaggatag atatcatgga ataaaataat tgccccatcg
     7801 tatactgttg cttggatctt ctgcaaaatc aaatcaggat ttcttgtctt ccaatcttct
     7861 gaatcaacag accattcgat gatcggtcgc tggatgatcg ttgctcctac attcgtgaca
     7921 gaaccatagg gcggacgaac aaaagtgggg gttttgccag tgactttttt gatctcagtg
     7981 tctgctttcg tcacttcctc cgtcatcgac tgggcagaaa gagtggccag attctgatga
     8041 tcgtatgtgt gattgccaat ctcgtgccct gcttcagctg tctgcttcac gatttcagga
     8101 tgctgcgccg tattttctcc taagacgaaa aaagtggcat gcacgttttt ttcttttaag
     8161 attcgcagaa gctgcggtgt tgtggtcgct cctggtccat catcaaagct gattgcgacg
     8221 cttttgacag ccggatctcg gctcgttttt gtactgtctt gtgttgattg tgatgactga
     8281 ctttgtacct tttccgctga ttcttgaatg gcttctttga cgttagctgt ttcagttgat
     8341 tggcttttaa tggttgattg ttctgattgt ttgaaagatt gtgacgaatg ctccgaagag
     8401 tgtgtttgtc cttttacttc ggtgatcaag gaggccaccg aagtaatttc gaaaaacaaa
     8461 agcacggata aagcaataat actgataact aaataatttc ttttcactat atctcttctt
     8521 tctctttttc tcttcttatt atttacttgt acttacttga aaattgaaac tgctagtcgc
     8581 aactacttct cgtctaatga tccaaataat cttccgatgc caaaatcgtt tttgtcacgg
     8641 acttaggaat gacaaattct ggtgtaccca tataaccagg agcaatttcg tattgagaaa
     8701 aagtcaaaac taattgatgc tctttgttca aataaaaact tcgtgtaggc gtaacaagtg
     8761 ttggtttttc tatcgtccct tcttgaatat cttgctcagt ccaatagtac ttagatggat
     8821 cttccttgac ttgttcggcc atctgccgtt tgacttcttt tgtcaaaaca gatagatagg
     8881 tgtcatcttt gaataaaagt ggaagtgaga gctggacact gtttttcttg tcgattgtgt
     8941 cgtatctttt ttcaacatgg ctatctgctg ctgtttgggt gatctgcctt tcaatcacga
     9001 gaaaccggtt atcgtctacg attttctgat aatctcccaa aacttggtaa tgatccgttt
     9061 caaaatttcc atattctttt tcagcttttt caaaatctgc ttgtccttcg cgaaagtatt
     9121 tttgatttaa tccatgaatt gttttatttt ctttctcatt ttgtgtattg atcttcggta
     9181 cctgaatatc gattttttca gatcgatcag taaagcgttc gcctaagatc aattcaatga
     9241 ctgatcctaa gatgggtaaa tcttctgcaa agcttcgaac ttgactatta aaaagaaggc
     9301 tacttgttgt aatcattacg atagccaaag atactccgaa cccttggaga cgtctctttc
     9361 tccgctttgc ttttataaat gattgctctt cgataataaa acgctggcgg atctcctctt
     9421 taagcccttt gggaattgct tgttctttat aagttttctt tagttttttt aaattttctt
     9481 tttgggaata catggcactc ttctctcctt cattctagga tttgacgtaa agcattcaat
     9541 gagcggtaca ttttcgtttt aactgtgttt tcattcatat ccaagatagt cgcaatttct
     9601 ttcaaacgaa atccttcaaa aaagtagagg atcagaatct ctttttccgg actgtctatt
     9661 ttatctaaaa tcatatggag ctctgcaacg gaagtttcta aaggtaactg ttgggggata
     9721 tcttgaattt caaataagtc cacaggcgtc cttttttgtc tgcgccaact atctaatgcc
     9781 gtgcggatca atatccgata aaaccatgca ctaaaatgct cgatctcttt ttctttttca
     9841 aaatagttca gggctttatg aaaactatct tgcatcacat ctaaggcctc ttctttatca
     9901 tgtacgtaat tcatcgtgat acggaacatt ttttgataat cagtttcgat gagtccattc
     9961 agcaggtgac ggtatttttc gatctgccgt tttttcttat atccagtaaa aatcattctc
    10021 cactcctttc tgcctcactt aatatgacgt acaaggtgcg ctaaaagttt caattttttt
    10081 attttttatg tatttacttg ggatttttat atacaaaaaa gaaggtgtga caaaaatcgt
    10141 gtcacacctc cttattctga taggctgatc gtctctgtca caacctctcg taagaaaata
    10201 atgtttattc tttttcagag tcagaaaatt cttcctcttc ataatcttca tcatcatcgt
    10261 cttcgtctat gatacttaaa tcttcttcga tatttcctgg caagtcttct tcatcatctg
    10321 tcgggtcatc tgctccgatt tcttgcaagt cagaattata agcagcgatt tcttcatctt
    10381 catcatcatc gtcatcaaac agatcatcgt catcttcttc tgtcagatct gtatcttctg
    10441 ggtcatcgtc attgtaatcg atcgcgtctt catcatcatt gataaaggcg ttgactttct
    10501 tgcgtttacg gcgacgtgga gtatcttcat cctcttcgtc cattccatgg tttacttctt
    10561 catcgatcga atcgatagca taccatgaac gcaagcccca acgattatcc cctaatgaga
    10621 taaagctgcc gtcaatattc aagtcagtat aaaattgtgc gagtgaatca cgaatttcac
    10681 tgtctgattt cccaagatag ttttggatct ggttagctaa atcagaaaaa tccatgacat
    10741 cgccacgttg ttctaagatc gcgtgtgcta cttcgatcat tgataattcg cttttgtttg
    10801 caccttcaaa tacactaatt tccaatcagg acacgtcctt tcacagtcta ctccttatca
    10861 tacaaaatca gacacaaaaa atcaaacttt tcttttatat ttacgctgta aaatccaagg
    10921 tcaaataata ttcgccgatt ttttccggtc ccatgaacag atcataatcg attcgaacct
    10981 tccctgctgt gggacggtct ttcaaactaa catgcagttc tttcgtaaaa gtagcaatct
    11041 ggatcatacc gtatggtgtt cgataggatg tttctaaccg ttctttatag ccaaatttca
    11101 aacgcatccg catttctcct gcacggatca gctgtacatg accatctgga actaatttga
    11161 tcgttacagg aatctcatcg cccgtctcct cctgaatctc tttataacgt atgtacagcg
    11221 tatcgccaat tgtcaccatt tgccctttca aatcaaaata aaaatcttgc acttcccctt
    11281 gctgttttac ttttgtacgc aatttgattg aaatcggtgt tccttgtgat aattccatta
    11341 ttcattctcc tttacttctc atagctacat tgttcctagt ttcttttgtt ttgtcaacca
    11401 acttgtccat ttttacagaa aacctttttt ccaaaacaac tctttgattt tagagtataa
    11461 tattgtagaa aatatgaatt tatacaaagg agccggttga atgaaccctg cgattcgact
    11521 agatcagcac atttatcaaa aaaacgactt ttcccctttt gcattaacag atatattaac
    11581 ggaatattct aaagaacacc agcaaatctt tttacatttc tggcaatatg actgcacagt
    11641 catcttaggt atgaaggatg ttcgaactcc ttttctaagt gacgggatcc aaacactgga
    11701 agatgcaggt tacgctccag tgatcagaaa ttcaggtgga ttagggatcg tatcagatga
    11761 aggaattttg aatatttctt tgattttccc ccaaagtgac acaaaaaaaa taaccataga
    11821 tgacgcttac gagcaaatgc tcctattgac taaatacgct tttccaaagg caccgataca
    11881 agcattcgaa atcacccatt cttattgtcc tggaacttat gatttaagca tccatggcaa
    11941 aaaatttgcc ggtatcgctc agcgtcggat caaaaatggg atttctgtta tgatgtactt
    12001 aagtgtcaac ggtaatcagg aaaaacgtgg gcaagtcatg aagcgatttt atcaatctgc
    12061 tttaaaagaa gactttggta caaatggcta tcctgcagtg gatcctgcta caatggcaac
    12121 acttgaagaa ctgcttgacc aacccctttt tgtaaaagaa gtcaaagaag catttgcaaa
    12181 agcttttgaa aagctgtatt ctgcaagtga tttacaggat tctaccgact ggctaaaaaa
    12241 agaaatccag ctggagcaat ggaacaacca aatccagaga atggaacaac gtaataacat
    12301 aaaggagcta cattatgaca accccttata aatatcaaat gctgcctatg gaaaaagttt
    12361 ttcgtgatcc agtccataac tatatccatg tccagcatca agtaatctta gatttgatca
    12421 attcgaaaga agtccaacgt ctacgacgca ttaaacaatt aggcacttct tctttcactt
    12481 ttcacggagc agaacatagc cgttttactc attcattagg cgtttatgag atttcaagac
    12541 gaatctgcga tatcttttct cgaaattttt caaaagaaaa aataggcaac ggaggctggg
    12601 atgatcagga acggctggtg actttatgtg cggcactgtt gcatgatgtc ggtcatggtc
    12661 cttactctca tacatttgaa catatcttcc atacgaatca tgaagccatc actgtagaga
    12721 tcatcacttc tccagaaaca gaagtgtatc agattcttaa ccgagtagaa gaaggttttc
    12781 ctgaaaaggt agctagtgtg atccaaaaaa cttaccccaa cccacaagtt gtccaaatga
    12841 tttctagtca gatcgatgca gatcgaatgg actatttgct tcgggatgct tatttcactg
    12901 gaacagaata tggaacattt gatcttaccc gaatattacg tgtgattcgt ccttatgaag
    12961 gcgggatcgc tttttccatg agtggcatgc atgcagtgga agattatatc gttagccgat
    13021 atcaaatgta tgtgcaagtg tacttccatc ctgtttcccg tggtatggaa gtaattttgg
    13081 agcatctgct tcatcgcgca cacgagttgt atcaagaaga tgccagcagt tttgaactcc
    13141 attctcaatt acttctgcca tttcttgacg gaagctttac tttagaagaa tacctcaaac
    13201 ttgatgatgg ggtcttgggc acttacttta ctcaatggtc tgaatctgct gatcctattt
    13261 taaatgattt agctaaacgt tttttgaatc gtaaaccact gaaatctgct actttttctg
    13321 gcaaccggga ttcaaaatta gtccaagaac tcactttgtt agtagaaaag gtcggatata
    13381 atcctgttta ttacactgct gtgaattcta gctacgatct accttatgat ttttatcgcc
    13441 cagaacaagg cagacatcgc acacaaatcg agattttgcg aaatgacgga actttgatag
    13501 aattatcaca ggttagtcag cttgttgctg cattagcggg acaagaacaa ggagatgagc
    13561 gatttttctt tccaaaagaa atggttgatc cgtccttgcg tgatcattat gatttatttg
    13621 acgaaactta tcaagaattt gccagccata tccgtaatgg cgcattgatt gaaataaatt
    13681 aaaatatatt ggaggaaatt atgtctatca aactagtcgc tatcgacatc gatggtactt
    13741 tattaaatga gaaaagagaa gtaacaaaag aagtaaaaga agcgattgcc gctgctgttg
    13801 caaaaggtgt ttcgatcgtt ttatgcactg gccgtccctt accaggtgtc caggaacagt
    13861 taaacgagtt gaaccttttt caagataatg attacgttat tacttataac ggggcactcg
    13921 tacaacaaac gaaatcaggt aaaatcatcg cccgtcacgg actgacacat gaagatttcc
    13981 tagagatcga agtaatggca cgccgtgtcg gcagtcatct ccattcgatc gatgacaaag
    14041 cgatctacac gcctaatcga gatatcagcg cttatacgat ccacgaagca agtttagtca
    14101 agatgccgct aaaatatcga acacctgaag aaatgacacc agacatgaac atcgtcaaaa
    14161 tgatgatgat cgacgaacca gaaattttag atgcagcaat tgcgcgttta ccgcaaactt
    14221 tccgtgataa atacacaacg gtcaaaagtg cgccctatta cttcgaagta ctaaataaag
    14281 aagcaagtaa aggtgcggca gttgcgaatc tcgcacagca ccttggtatt gatcaagatg
    14341 aaatcatggc tatcggagat aacgagaacg acttatccat gattgaatat gctggtctag
    14401 gagtagcaat gggaaatgcc gtttctcttg tcaaagaagc agcaaatgtc atcacttcat
    14461 caaacgacga gcacggtgta gctgaagcaa tcaaaaaata tgtactgtaa taaataaaaa
    14521 aactgagaga aagctgatct actcgctttc tttcagtttt tatttttttc gatccatctc
    14581 tcgactgccc atcggtggct accacgtttc aatttcacaa gcgcttcttg agggctcacc
    14641 caccaaattt tattggtctt ttcagttggt tctcctatac gcttccaaga atcagcaaca
    14701 aagaaatatc ctggattata ataataagtt gcacgataat tcgagtaaaa atactcatca
    14761 gcttctccca gataagaaga aatttctgcc acaattccca tttcttctat cagctcacgg
    14821 acaatcgctt cttctttcgt ctcttctcct tcaatttccc cgccaggaag aaaaaaagca
    14881 ccatttggag cctcgattac tgcaatctgc tgatctcttt ctatgacaat atatgctgca
    14941 taacgggttt gataatcagc aaattgcttt ttttcaccaa aaattggttt atccatgaat
    15001 agtttccacc ttaacagttt tattttctat ccattttaca tgagaaattt ctcatgaaca
    15061 agcacaaatc aactatttcc ttacttcttt ttgttatgat tgagtaaaag gaaaaggaga
    15121 gatgaatcta tgaaaatggc tcacacatgt gttcgagtga aagatcttga agcatcaatc
    15181 gaattttatc aaaaggcatt tggttttgaa gaaagccgcc gccgtgattt tccagaaaac
    15241 aaatttacac ttgtttattt gacacttcct ggggatgact atgaattaga gttgacttac
    15301 aactatgatc atggtgcgta tgatctagga gacggctatg gtcatatcgc tatagcagct
    15361 gatgatctag aaaaattaca cgagcaacat aaagctgctg gtcttgagat tactgactta
    15421 aaaggattac ctggcactgc gccttcttac tactttgtca ttgaccctga tggttataaa
    15481 atcgaagtca tccgaggata gttagtatgg ggcattttac tcttgattga ataaaaaaca
    15541 aacaccaata attaaaatga gacgcaaact tttcattaaa ttgaaaaagt ttgcgtcttt
    15601 ttttaacaac cattgtagct ataaggttgc cactctccac caatttattt ttaattcatt
    15661 tgaaagaaat caaataaacg aggtaccttt agaaaacaaa actgcttttc ctcctattat
    15721 cacacgatct tcttttaatt cacattctag atccccgccc cttgatgaca tttgataagc
    15781 tgacaatttt gttttattca ttctttctgc ccaataagga ataagattag aatgtgctga
    15841 tccacatact ggatcttcat tgattgttaa ttttggaaag aaggttctag aaacaaagtc
    15901 tttttgcaca ctttctgctg tcacgatgac tcctattcct aattcaaaat ttttgatagc
    15961 tgtaaaatct ggctgcaatt tagccaccgt tttttcatct tctaatacga aaaataaatc
    16021 acgtgctaag taggcttctt taatcttagc cccaatcgct tcttcatatt cagacaatat
    16081 cggcactttt ttcggcataa tactaggaaa atccatgtaa taataatctt taattcttgt
    16141 cacaaataga ttaccgcttt gagtagaaaa tttaatcgtt tcatcaggta ttttgtaata
    16201 gttaaataaa acaaatgctg ttgctaatgt cgcgtgacca caaagatcga tttctcgatc
    16261 tggcgtaaac catctaagct caaattcttg gtttttcttc actgtaaatg ctgtttcaga
    16321 taaattgttt tcaatggcaa ttttttgcat cgtaccttct ggcagccatt cttctagcac
    16381 ataaaccgct gctggatttc ctttgaacac ctcatctgca aatgcatcaa ctacgtaata
    16441 atctaagttc attttaaagc ccctttctct aatttgttat aaatatattc tgcacaacaa
    16501 agatcttcaa tagctagacc aactgcgtcg aaaatcgtcg gcctcgattc tcctttataa
    16561 aatgtttttt tctcagaaac taattcgcct aatgtaccaa caatacaaga ttcctctaaa
    16621 tctccttctg ccatggctat aagcaggtcc ccgctttctg ttaaggcagc tgagtaatca
    16681 tctacataga tttgagaagt ttccatcaac gatgtactta tctctcttgt atcaggagta
    16741 aatgtgccca cagcattgat atgtgcgtct tcccttaccc actctttttg aagaaatgcc
    16801 tctttactag aggtcaacgt acaaatgatt tccgctggac gtactgcatc ttctactgat
    16861 attgcattta taaacgttat tttaggatac tgttttttag ctttagcgat aaaagtattt
    16921 cgtctttctt ctaaaatatc gtaaacaaac actgtagtga tgggacgaat ggtagaaatc
    16981 gcagtcaaat gcgacaatgc ttgttgacca gctccaatta aagcaagtgt ttctgcatgc
    17041 ggatttgcta agtaatctgt tgccagcgca gatacagcag ctgttcgaat ccaggtgact
    17101 gcatttgcgt ttatcaatgc tttgggtagt ccgttttgag catcaaacaa gaaaatctct
    17161 cctaaatgag aaggtaaatg atactgatga ttatccggaa aagcggtaat gacttttgtg
    17221 ccaaaatatc tgttctcccc caaataagca ggcatcatcg caaacatatt tttgcctttt
    17281 ccgtcaggta aaatttccac caatcgagga ttttggctga ttttaccact ttcataatca
    17341 aaaaaacatt ttttcattac ttgaatcgct tcttcaaaac taaaagaagc tactacttcc
    17401 tcaaaattaa taattttcat agacaattcc ctcctcacaa aagattaact tctaaaatca
    17461 atacaatcaa tggtaaaatt gtatggtata caattttaag gagtggagaa aatgcctatt
    17521 aattcttatg acaattaccc gttaacttgg cgaccagaca aacaaaaatt gaagcgtcct
    17581 tattatcgat cgttgattca acaattagaa caagatattt taaagaataa attacagaaa
    17641 aacactcagt taccctccca aagagagcta gctgattatt tagatttaaa ttttactacc
    17701 gttggcaagg cttataaata tggaattgag aaaggtttgc tgtatacgaa tattggaagt
    17761 ggcacattta tttctcccaa tgtctttgag tccatcacca tctctaccaa tgaagtaacc
    17821 gaacatatca ttgattttgg tctggttagt tcttttgaag aatgtaataa attcgttgtc
    17881 ccgtttattc aatcaactag taataaccca aatcttgttg gtttgcttaa ttaccaagat
    17941 cccaaaggca caaattttca attgactact gcagcaaatt ggttaaaaac tcaaggagtc
    18001 attccgtcct tagaatctct atctattgtt tccggtgttc aaaatggatt ggctatcaca
    18061 ctttctgcac ttttctctcc aggtgataga atagcaacag accgatatac atacgctaat
    18121 ttcattgaat tggctcagtt atatcattta gagatcgttc caattgattt tgacgagcaa
    18181 ggaatgcgac ctgatatatt gattcaggaa tgtagaaaaa agaagattca tggtgtcttt
    18241 cttatgcctt cttgtaataa tcctataggg tttcaaatct ctcacaaaag acgagagcaa
    18301 ttagccgaag tgattgaaaa agaaggtcta tgggttattg aagatgatat ccattcattt
    18361 ttaacaactt actatcaaca tacgatcatt cctcctttcc aaaatttatt acctaaccaa
    18421 acaatctact tagctggaat gacaaaattt atttgttctg gattgcgagt tgcttatctt
    18481 gtagttccag tacatgtcca aagcaagata gaacaagcaa tttttaatat aaatgtgaaa
    18541 acttctggat tagatgtgga agtcatcact caaatcctcc attcaaatga agcaaatagg
    18601 atcttagaaa ctaaattaag tcttacaaaa agagccaatc agctttttga ttctcttttc
    18661 aaagtagaaa aaccaacgaa tcgtgtccct tactatcgaa ttcttccagt tgatcctgag
    18721 attgacccta agataattga gaatgacttt cttgctaacg gtatccgact ttatcattcc
    18781 agacgcttta ctacacgtgc gcaaccagat gcattcgttc gaatttccct atcctctaat
    18841 aaccttgaaa ttttag
//
LOCUS       DAJSKS010000045        18529 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJSKS010000045 DAJSKS010000000
VERSION     DAJSKS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.046"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            124..771
                     /locus_tag="ON396_001334"
     CDS             124..771
                     /locus_tag="ON396_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCR3113427.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    900..1116
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1164..3809
                     /locus_tag="ON396_001335"
     CDS             1164..3809
                     /locus_tag="ON396_001335"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR3113428.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4119..5432
                     /locus_tag="ON396_001336"
     CDS             4119..5432
                     /locus_tag="ON396_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR3113429.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5422..6075
                     /locus_tag="ON396_001337"
     CDS             5422..6075
                     /locus_tag="ON396_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3113430.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6130..6822
                     /gene="radC"
                     /locus_tag="ON396_001338"
     CDS             6130..6822
                     /gene="radC"
                     /locus_tag="ON396_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR3113431.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6972..7172
                     /locus_tag="ON396_001339"
     CDS             6972..7172
                     /locus_tag="ON396_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3113432.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7426..9105)
                     /locus_tag="ON396_001340"
     CDS             complement(7426..9105)
                     /locus_tag="ON396_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR3113433.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9107..9319)
                     /locus_tag="ON396_001341"
     CDS             complement(9107..9319)
                     /locus_tag="ON396_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCR3113434.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9712..11442
                     /gene="efrA"
                     /locus_tag="ON396_001342"
     CDS             9712..11442
                     /gene="efrA"
                     /locus_tag="ON396_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HCR3113435.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11439..13199
                     /gene="efrB"
                     /locus_tag="ON396_001343"
     CDS             11439..13199
                     /gene="efrB"
                     /locus_tag="ON396_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HCR3113436.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13294..13491
                     /locus_tag="ON396_001344"
     CDS             13294..13491
                     /locus_tag="ON396_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113437.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13644..14129
                     /locus_tag="ON396_001345"
     CDS             13644..14129
                     /locus_tag="ON396_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR3113438.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14233..14826)
                     /gene="clpP"
                     /locus_tag="ON396_001346"
     CDS             complement(14233..14826)
                     /gene="clpP"
                     /locus_tag="ON396_001346"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR3113439.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            15006..15533
                     /locus_tag="ON396_001347"
     CDS             15006..15533
                     /locus_tag="ON396_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113440.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15624..16361
                     /locus_tag="ON396_001348"
     CDS             15624..16361
                     /locus_tag="ON396_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR3113441.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16365..17282
                     /gene="miaA"
                     /locus_tag="ON396_001349"
     CDS             16365..17282
                     /gene="miaA"
                     /locus_tag="ON396_001349"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR3113442.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17284..18513
                     /gene="hflX"
                     /locus_tag="ON396_001350"
     CDS             17284..18513
                     /gene="hflX"
                     /locus_tag="ON396_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR3113443.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5990 a         3204 c         4027 g         5308 t
ORIGIN      
        1 gatgcaagct tttgaacacc gttttatttg tctcacaccc ttttccttcc tacttgtaac
       61 cttgctcccg tgttagaaac atgttacact aaatatgtga aattattaac aaggagcgtc
      121 gttatgcgag aaaaaacaga tacactgcca aaagcaactg cgaaaagatt acctttatat
      181 ttacgctact tgaagatgct ggatgactca ggtataagta gaatcaaatc aaatgagttc
      241 agcgaaatca cccaaattcc ttctgcaacg atccgcagag acttctcgca attaggcgag
      301 ctaggcagaa gcggatatgg ctatgatgtc ccttttctta tcgatgtatt caataatatc
      361 cttaatacta aagaagaaaa acgaatcgca ttagtaggat atggaaatct tggcaaagca
      421 ttgaaacaca acaattttag aagaaatgaa aatctaaata tcgtgtgcgt ctttgataat
      481 gatccagcac tgatcaatcg agtaattgat ggagaaatga tctatccgat tgatcgtttc
      541 gcggagatag ccaaagcaaa aaatgtgaca gtagcaatct ctacggtacc aagtaaatat
      601 tcacaatctg ctattgatga aattgtcaaa ggaaatgtaa ccgctatact taattttgcg
      661 cctgacagag taactgtccc agcttatgtc aatgttcaat acattgattt gacaaccgag
      721 ttgcagacgc ttatttactt tgatgagaat tactcagaag ttttttctta ggaaaatttg
      781 agtaggctta ttgatcttgc gagaactcgt cgtaattctt tcaaaaattt tttagtacta
      841 aatgaagtgg ttttttcacg accacttgtt gactaaaaaa aaaaaccatt gtagaatgaa
      901 gcataacgct gaacaagagg agtaactggc acattcattc ttagagagag aactatctgc
      961 tgaaaggttc tcaatgacaa ccagtgaagg tagcttggga gtttctgtct tgaatggagt
     1021 agagatagac gagtcgtgat cgttaactca cttgagaggc aataatgatt gattattgca
     1081 atttaggtgg taccgcgtaa atcatacgtc ctagaatacg aaagtatttt aggacgtttt
     1141 ttagtttaaa ggaggaaaac agcatgacgg aagaaaataa cctatcaaca aaatataatc
     1201 cgcaagaagt tgaagctgga cgttatcagc attggttaga acaagatttg tttaaaccga
     1261 gcggagataa aaaagcaaaa ccttattcaa tcgtaatccc gcccccaaat gttactggaa
     1321 aacttcattt agggcatgct tgggatacga ccttgcaaga tatgctgatt cgtcaaaaga
     1381 gaatgcaagg atatgatact ttatggctgc ctggtatgga ccatgcagga atcgcgacac
     1441 aagcaaaagt agaagcgaag cttagagagc aagggatttc tcgttatgat ctaggtcgtg
     1501 aaaagttcat cgatcaagtg tgggaatgga aagaagagta tgcgagccac atccgcgaac
     1561 agtgggctaa aatgggctta tctttagatt acagtaggga acgttttaca ttagatgatg
     1621 gattatctga agcagtacgt aaagtttttg ttaccttata cgaaaaaggt ttgatttatc
     1681 gtggtgaata tatcatcaac tgggatcctc aagcacgaac agctctatct gatatcgaag
     1741 ttattcataa agatatcgaa ggcgcctttt atcatatgag ctatgaacta gcagatggat
     1801 caggcgtagt agaaattgcg acaacccgtc cagaaacgat gttgggagat acggcgattg
     1861 ctgttcatcc agaagacgag cgttatcaag cgctcatcgg caaaaaagtg attctacctt
     1921 tagtcaataa acaaatccca atcattgcag atgaatatgt agatatggaa ttcgggacag
     1981 gtgtagtaaa aatcactcca gcacatgatc caaacgactt tgaggtaggt aatcgtcatg
     2041 atcttccacg cgtaaatgtt atggatgatg atggtacgat gaatgaacta gcaggaaaat
     2101 atgctggaat ggaacgtttt gctgctcgta aagcaatcgt gcaagatctg aaagaaatcg
     2161 gtcgattgat taaaatcgat acgatgatcc acagtgtggg tcattccgag cggacaggtg
     2221 tcgttgttga acctcggctg tctactcaat ggtttgtaaa aatggcgccg ttagcgaaaa
     2281 aagccattga taaccaagaa acagatcaag cagtggaatt cttccctcca agattcaatc
     2341 aaacgttttt acgttggatg gaaaatgtcc atgattgggt aatctcacga caattatggt
     2401 ggggacatca aattcctgca tggtatcaca aagaaacagg tgaaatgtat gtagggatgg
     2461 aagcaccagc ggatagtgaa aactgggtac aagatcctga tgttttagac acttggttta
     2521 gttcagcttt gtggcctttc tcaacaatgg gctggccaga tacggaaagc gaagattata
     2581 agcgatactt ccctactagc actttagtaa ccggttatga catcattttc ttctgggtaa
     2641 gcagaatgat tttccaaagc ttggaattta ctggcgaacg tccatttgag aatgtcttga
     2701 ttcatggatt gattcgtgac gaacaagggc ggaagatgag taaatcatta ggtaatggaa
     2761 tcgatccgat ggaagtaatc gaaaagtatg gagcagatgc tctccgctgg ttcttatcga
     2821 atggttctgc accaggacaa gatgtccgct tcagctatga aaaaatggat gcttcttgga
     2881 actttatcaa caaaatctgg aatgcttcac gtttcgttat catgaatatc gaaggaatga
     2941 ccgtagaaga gatcgattta tctggagaaa aatcagtggc tgaccggtgg atccttactc
     3001 gtttgaatga aacgatcgaa cgagtaactg agctatttga tcgttttgaa tttggtgaag
     3061 caggtcgaca actgtataac tttatctggg atgatttttg tgactggtac atcgaaatga
     3121 gtaaagaagt actttatgga gatgatgctg tttcaaaaca aaccacccgt agcatcttag
     3181 tccatacact tgatcagatc ctacgtttat tgcatccaat catgccattc gttacagaag
     3241 aaatatggga acatattcct catcagggaa attctctagt agtggcagaa tatcctgttg
     3301 tacgtccaga gtttgatgat gaaacagctt ctaaagggat ggaagtattg aaagaattga
     3361 ttcgagccgt tcgaaatatt cgttcagaag tgaatacacc attgtctaaa ccaattacgt
     3421 tgctaattaa aacaaacgat gctaaaatcg atcaattttt agttgaaaat acaagctata
     3481 tcgaacgctt ctgtaatccc gaagagttga cgatatcaag tgagatcact gcgccagatc
     3541 tagcgatgtc agctgttctg acaggtgcag aaatcttctt gccgttagca ggattgatca
     3601 atattgaaga agaaatcaaa cgattagaaa aagaactgga aaagtggaca agcgaagtaa
     3661 aacgtgtcca aggaaaactt tctaatgaac gttttgtttc taacgcgccg gaagaagtag
     3721 tagaggcaga gcgtgcaaaa gaaaaagatt atttagagaa gcaaacggct gtcaaagaac
     3781 gaatcgaaag tttgcgtact attcaataag aatagaaaaa gagagtgtga aatcagctag
     3841 ctattactaa aagctagctg attgaacatc gttacaggaa agagtttgag cctcactctt
     3901 agcttcgatt cataaggaga tgtgacataa attttgtcgc atctcttttt tagtctactc
     3961 ttattatgta ggagaaggag ggaggtggat catgtatcaa gagtcaataa agtagttgat
     4021 aaaatcgttc ctctctctgt ttcttttctc tttgtcatac ttttattttt cttatgtgtt
     4081 ctatttgtta taataaaaga gaaatggggg aattgttgat gacgattgaa gaagcaatag
     4141 aatggatcca tagtcgatta ccatttggtt cacgtcctgg ccttgaccga gtcgaagctt
     4201 tattagaagt ggtagggcat ccagagagaa aagtaaaaac gatccatatc gctggaacaa
     4261 acggaaaagg ttctactgta gcttatttga gaagcctatt agaagaggct gggctgacag
     4321 taggtacatt tacttcacct tatatcgaaa cgttcaatga acggatcgca atcaatggca
     4381 aaagcattcc agacaatgat ttgatccagc ttgtaaaaaa atatcagcca ctgatcaaac
     4441 aacttgatca atttgaagca gtagctggta ttactgaatt cgagacatta acagcaatgg
     4501 catttgatta ttttttgatg aagaaagtgg acgtagcgat tatcgaagtt ggcttaggcg
     4561 gcttattgga ttctaccaat gtagctgtac caatgctgac gggaataaca acgattggac
     4621 ttgatcatac cgaaatactt ggagatacaa ttgaagaaat cgctgcacaa aaagctggaa
     4681 tcatcaaaca aggcgttcca gtagtaacag gaaatattgc cccagaagct tttactgtca
     4741 tccaacaaac agctaaagca aaaaatgcac cgctttatgt atacggggaa aattataaaa
     4801 taaactatct gcatcctgat gagatgtggg gagaagtatt tgaatttttt aatgaaatag
     4861 gaaagatccc tcaattaacc actccgcttt tagggcaaca tcaagctgaa aatgcaggag
     4921 tagccatcga attgtatttt gtttactgtc aattggaaaa gctgcctttc cgagaaaaag
     4981 atgttttgaa gggattgaag aaatctcgat ggccaggaag aatggagcgt ttgagtaacg
     5041 agccattagt tgttttagat ggtgcccata atacgcatgc aatgaaccgg cttgttgaga
     5101 atttgaaaaa agaattcaaa ggatatcaaa tcaacatttt gttttccgcc ttacgaacta
     5161 aaaatgttag agaaatgcta gaacgattgc agcaagtacc aaatgtccat atttatttga
     5221 cgacctttga acatccaaaa gccatcgaat taagtgaagt atcagattta gcaagtgaga
     5281 gagtatcaat cgtttcattg tggcaattcg gattagcaga tatattggaa aagatgacaa
     5341 atgaagacgt attagttgtg accggttcac tttatttcat ttcagaagtt cgccatttat
     5401 taatacaatt aggaggagat catgaagtat aatgcagtga tttttgacat ggatggtttg
     5461 ctgttcgaca cagagttagt gtattacgaa gccacacaag tagtagcaga caagatgggt
     5521 tttccttatg ataaagacct gtatttaaaa ttcttaggtg tttcagatga agaagtatgg
     5581 gcgaattatc atatgatttt ctcggaattt ggcaaaacaa gtgtacaaag attcatagat
     5641 gattcatacc aggaaacgct tgaacgtttt tcgcaaggag cggtacaaat gaagcccggt
     5701 gtgttggaac tcctctcttt tttagaagaa catcagattc ccaaagttgt cgcttccagt
     5761 aaccaacgag cagtcattga acttttattg aagaagaaca atctgtttga acgatttgag
     5821 acgattgttt ctgcagaaaa tgttcaacga gcaaaaccag acccagaaat tttcttgtta
     5881 gctaatcaga agctaggtac agcgaagaaa gaaacattga ttttagaaga ttcacaaaat
     5941 ggtattttag cagcttctgc ggctgagatc ccggtagtca tggtaccaga tctgcttcca
     6001 ccatcccaag aattgatcaa aaaaacagct ggggttgttt cttctttaca tgaaatccct
     6061 gcattgttaa aataaaaatc catctctttt cgtatcttat tttagataag atagaaagga
     6121 gatgtttttt tgggaaaacg attgatcaaa gaagtaccta ctagctcttt accaagggaa
     6181 cgtatggaaa tttatggcgc ggaagcttta tcggatcagg agcttttggc aatcttactg
     6241 cgtaccggtc aacaccctta tagtgtgatg tcgattgctg gaaatctgtt aaaaacgttt
     6301 ggaggactgg cctctttacg acaggctact ttgcatgagt tagaagagat tcgagggata
     6361 ggacgcgtga aagcaatcga aatcaaagcc ttgatcgaat taggtcgacg aattcagacg
     6421 gatcatgaaa gagcggctcc accggttcgt accagctacg aattggccca gcaattgatt
     6481 ctggaaatga aagatcacaa acaagaacat ttggtttgta tttatttgga cacgaagaat
     6541 caagtgatct taaaaaaaac agtgttcatc ggttcgctga atcagtcagt cgctcatcct
     6601 agagaaattt tccattatgc tgtacgttat tgtgcagcaa ggatcgtttt agcgcataat
     6661 catccttctg gtaatgttat tccatcgcag caggatatga attttaccaa aaggatacaa
     6721 aagtgtggag aaatgatggg gatcactgta ctggaccact tgatcatcgg acgcaaacga
     6781 tattttagtt tgagagaaga aggaatgatg gaagagaagt aagttgcttt cttgcaaaaa
     6841 aaatgaagca agtgtacaat tatattgtaa ttttgtttgg gaatagaagt tgaaacatct
     6901 gttgcacctc tatcgtacac gagcaatgtt acaatttaaa ttagtgcttt tggcacgagg
     6961 aggatattat tatggaaaac ggaacagtaa aatggttcaa cgctgaaaaa ggatttggat
     7021 ttatctcacg tgaagatgga agcgacgtat tcgttcactt ctcagctatc caaggtgacg
     7081 gattcaaaac attagaagaa ggtcaagcag tgactttcga cgttgaagaa tcagaccgcg
     7141 gcccacaagc tgcaaacgtt gtaaaagctt aattttatct gtaaatcaaa ttgatggtag
     7201 acatcaattt ttacgagaga acaaagcgag taacaaaaat acctgccaca agagacaatt
     7261 tctcttgtgg caggtatttt tttgtatgaa aaagtaaata aatttaagag gctatgaaag
     7321 agccaccttg tatcaaggaa taagaacaaa caaaaatggc ttctcatgtt tttcatttgt
     7381 tttctcttat attcaggaca aggtgctgat ttcataacct cttatttatt cttcatctgg
     7441 tgtcattacc attggcaaaa tcattggatg tctttctgta cgttcgaata agaaaggctg
     7501 aagcgcgcca gtcatcgctt ctttcaattt atattctgaa cagtcaggtc cattcaaaca
     7561 ttctcttaaa gcgtggaata agattcgctg agcttcattg atcatttctc ctgattctcg
     7621 catgtagaca aatccacgag agagaatatc tggaccagcc aagatttctt ttttcttgat
     7681 atccactgtc gcgacagcta atacaagacc ttcttctgaa agcaggtgac ggtcacgtaa
     7741 aacgacatta ccaatatcac cgatcccgct accatcgata tacacatctc cagccacaaa
     7801 atgcccagca ggacgcgcag aatcgctagt taacgctaaa acatcgccat tttccatgat
     7861 gaagcagttt tctgcaggaa cacctacatc ttgtgctaga gaagcatgta ctttcaacat
     7921 gcggaattct ccgtggacag gaataaaata ttttggcttc atcaaacgaa gcattaattt
     7981 ttgttcttct tgtccaccgt gaccggaagt atggatgtta ttgatttttc cgtgaatcac
     8041 ttctgcacca gcttcagaca gcaaattgat caaatgattg acacttgttg tattaccagg
     8101 aataggtgaa cttgaaaaga tcactgtatc gtctggctgg atttggattt gacggtgagt
     8161 accattcgcg atgcggctca acgcggccat cgcttctcct tgagaacctg tacataggat
     8221 catcgtttca ttggcaggca agcgattgat ttcgtgtgca tctacaaatg tgccgtctgg
     8281 aactttgata tatcctaaac gttgaccgtt gacgatggca ttttccatgc ttcgtccgaa
     8341 aacagcgatt ttacgaccag ttgccacagc ggcatctgct gcttgctgca aacggaagat
     8401 gttagaagca aagctggcaa aaatgatacg tccttcgatt tttgaaaaga tcttcaaaat
     8461 agaatgccca atcgtttttt ctgatttagt aaatgttgga atctcagcat ttgtactatc
     8521 agataaaagg cacaagactc cttcttcccc gatacgagcc attttatgca agtttgctgg
     8581 ttcgccaaca ggagtgaaat cgaatttgaa gtctccagtc gctacgatat ttccagaagg
     8641 tgttttcacc acgatcccta atgcatctgg gatactatgg gtcgttctga aaaagctgac
     8701 agaagtcttt ctaaaacgga tgatcgtatc ttcattgatc tcatggagct ctgcatctcg
     8761 aagcaagcca tgctcatcta atttgtttgt gatcaacgct aatgcgagag gaccagcata
     8821 gataggaata tttgcttgtt tcagcaagta gggtattccg ccgatgtggt cttcgtgccc
     8881 gtgagtaatc accaaggctt ttactttgtg taagttttgt atgatgtagc tgtagtcggg
     8941 aatgacatag tcaattccaa gtaaatcgtc ttccgggaat ttgatcccgg catcaatcaa
     9001 aatgatttca tcttggaatt gtaccccata cgtatttttc ccgatttcac ctaatccacc
     9061 aatagcaaag acgcctgtct cgttattttt gatggaaagt ttcatattaa aactccacta
     9121 atgagaaatc agcattttct ttttcgtact ctagatgact tccttctaga agttgaacga
     9181 attcaatatt gtatggggta ttttcttcca cacgttggcg agcgtctaca tcgctttttg
     9241 cctcaatgta aagagatttt gtttcttccc gtttagggtt gcgtactttt gtttcttgat
     9301 aataaacttt atagatcatt gttttgtctc ctttatctgc gtaaagcagt gatttagcta
     9361 gttgattttt ttagacaaca caaaacgagg ggacaagcca cctggttttt catatgcttt
     9421 gactagaata ctggattcga gtcaaagaga caagcctcgt ttttgcttat ctgttcggat
     9481 attttttcaa ccgtaccttc tgtataaaaa atatacaaca attaattgtt attctagcat
     9541 aaaacagcaa ataagtatag aaatcaacta gattgaaaga aaaaatcaat gtttatcatc
     9601 ccgctataat cataatgact tttttatgag agaagtgttc tattaatagg ctcgttgtgc
     9661 tatcattttt agtaatgaaa atgtaacaaa agattaaaag gagatgagaa agtgaaacta
     9721 atgtggcatt ataccatgcg ttataagaaa ttcttgtttt tcaatttcat atgcgtattt
     9781 ggttttattt tgattgaatt aggacttcct acgattttag cacgtatgat cgatgtagga
     9841 atccgcaatg atgattttga ttatgtgaag caacaaggga tatttatgat cgttattact
     9901 gtaatcggta ttattttgaa tatcttgtta ggttattttg gttcgaggat tacgactaat
     9961 atcgtagcag atattcgaga tgatttgttc gaaaagatcc aaagttattc gcatcaggaa
    10021 tacgaaaatc taggcgtatc ttcattgatc actcgtacaa caaacgatgc ttatcagatc
    10081 atgttgttcc tgcaaaatat tttacgtatc ggttttatgg caccaatgat gttcattgtc
    10141 agtttgtaca tggtcatgcg tacaagtgtg acactcagtt tgtatgtagt aggtgcatta
    10201 cctttactat tattggcagt cgtagcgatt gctaaggtgt ctgaaccgtt atctaaaaag
    10261 caacaaaaaa acttagataa aatcaacagt atcttgcggg aaaatctttc aggattgcgt
    10321 gtgattcgtg cattcgtcaa tgaaaagttt gaagaaaaac gttttgctgt agtcaatgag
    10381 aattatgctt ctagttcaaa aagcttattc cgtttgatgg ctgttgcaca accgggtttt
    10441 tatttcttat ttaatatcgt aatggtattg atcatctgga gcggggcagt gcaaatcgat
    10501 gcaggcgagc ttcgtgtagg agatttgatt gcgtttatcg agtatatatt ccatgccttg
    10561 ttctcattca tgttatttgc tagtgtgttc atgatgtatc caagagcagc tgtttcagct
    10621 ggacgtatcc aagaagccct agatgccaaa ccttcgattg tagaagatcc aaatggtata
    10681 tctgatacaa aaactaaagg gtatatcgaa tttcaaaatg taacatttgc ttatccaggc
    10741 cattcgcaag agccagtgat acgaaatgtc agttttacag cctctccagg agagaccgtt
    10801 gcttttattg gaagtaccgg tagcggaaaa tctacgttga tccaattgat ccctcgcttt
    10861 tatgatgtga gtgaaggaaa gatcttgatc gatggagtag acgtgagaga ttatcggcta
    10921 agcaagttgc gagataagat tgggtttatt ccgcaaaaag cgctgctatt tactggaacg
    10981 attgcagata atttgcgata cgggaaagaa gatgctacgc aagaagaaat ggaacgtgca
    11041 gctgagattg ctcaagcttc agaattcatt tctcgaaaac cagatggatt taatgaacat
    11101 ttatcagaag gtggttccaa tttttcaggt ggacaaaaac aacgattggc aatagccaga
    11161 gccatcatac gccgtccgga aatctatatt tttgatgaca gcttttctgc attagattat
    11221 caaacggatg cgaagttacg tgctcgtctg aaaaaagaaa cgacagaatc tactgtattg
    11281 atcgtagcac aacgtgtggg aacgatcatg catgcagata agatcatcgt attaaatgaa
    11341 ggagaagtcg tcggtatggg gacgcatcgg gagttgttgg aaaaatgccc gatttattat
    11401 gatattgccg cttctcaatt atcaaaggag gaattagcat gaaaaaaacc ttttcttcct
    11461 taaaacgttt agctcgatat atcagaccgt atcgtgagac ttttctttta gtgatcctat
    11521 ttacagttct gacagtggct ttcaacgtgg ctttgccata tgttacagga cttcctacta
    11581 cagaaatcag ccgtaatatt gccaatggcg aatcaatcaa tttttcttat atattcaaat
    11641 gtctcatttg gatcacagtt gttggaatag ggtattgtat ttcgcaatta ctttctggtg
    11701 ttttgatgac aaatgtcgtt caaagtgcca tgcatgattt gagaagagac atagaagaaa
    11761 aaatcaatcg tcttccggtt tcatatttcg ataaaaacca gcaaggaaat attttgtctc
    11821 gtgtgacgaa tgatgtagat gcagtcagcg gtgctttaca acaagctttt attggtatcg
    11881 taaatgcgat attgggaatt acgttggctg cagcaatgat gttttatatc cagccgatga
    11941 tggcgctgat ctctatgatc atgattccaa catcgatctg gatctcaaaa cgagtgatca
    12001 atgcttcgca gaaatatttt caaagcatgc agaattctct tggtgagcta aacggctacg
    12061 tccaagaaaa tatgacaggt tttagtgtat tgaaagtata tggacgcgaa aaagaaacat
    12121 tggaaggatt caaaaaagtt aatcacagct tgaaatactt tgggtttcgt gcagcattca
    12181 tttctggctt gatgatgcct ctagttcaat taactgccta tgctacttat attggtatgg
    12241 ctgttctagg aagcttttat gttattactg gggcgatcgt tgtcggtcaa ctacaagcat
    12301 tcatccaata tatttggcaa atcagtcagc caatggggaa cgtgacacaa ttgtcttcag
    12361 ttttgcaaag tgcatcagcg gcaacgaaac gtgtattcga gattttagat gagccagaag
    12421 aaaaagaaaa caaagtagat gcttcactgc cagaaacgat tttaggagat gtgacattcg
    12481 atcatgtcga ttttgcatat gatccgaaga aaccattaat ccaagatttg aattttgaag
    12541 tgaaagctgg tcaaaccgta gctgtggtcg gtccgaccgg tgccggcaag acgacattga
    12601 tcaatctatt gatgcgtttt tatgacgtag acaaaggagc aataaggatt gatggaatcg
    12661 acaccaaatc aatgagtcgc agtgatgtgc gttcgctatt tggtatggtc ttgcaagatg
    12721 catggcttta tcaaggaacg attgcagata atatccgctt cggaaagctt gatgcgacag
    12781 attatgaagt agttgatgct gcgaaaacag caaacgtaga tcattttatc cgaacgatgc
    12841 cagatggata tgatatggcc atcaattctg agggagacaa tgtgtcttta ggacaaaaac
    12901 agttgctgac gattgcccga gcagtggtct ccgatcctaa aatcttgatt ttagatgaag
    12961 caacaagttc agtagacacc cgtttagaag cgttgattca aaaagcaatg gatcgcgtga
    13021 tggagggacg gacaagtttc gtcatcgctc accgtttatc gacaatccgt gatgcagatc
    13081 tgattctagt gatggatcaa ggtcagatca ttgaaaaagg aacgcatgag agtttattgg
    13141 cacaaggtgg attttatgaa aaactgtata acagccaatt tgctgaggaa gcagaataag
    13201 tcatataaga ataaaacagt ttaaaactta ttttttgttc tctattcttg ataatggtaa
    13261 agtaaagtta tcaagaaaag tgaggacacg gagatggaac agtttttaaa tgaaaaaatt
    13321 actgaagcca aaagagaagt tgatcgttta acgcacgtct gtcagacgaa gccttttgat
    13381 tcagctgatt tttttgaaaa tgagtttcaa atcgaacgct tggcagcaca aatagaagtg
    13441 tacgaattaa ttttaaaaaa agtaatagaa gaaaatatag atgtacaata aaaacaggta
    13501 aaacgtcagt taccagatac tgtaaataga catctggcct gagcaggttt tacctgtttt
    13561 ttttcttatg aaataaatat atgctatgat acctttattc aataggatta gtttctacaa
    13621 aaaatgaaag gagaaaaata aatatggaac ggtttatagc atcaaatgcc acagtgattg
    13681 gtgatgtgac attgtcagaa gatgtgacga tctggtatca agctgtttta cgcggggaca
    13741 gtaattggat aaaaatagga caaagaacga atatccaaga tggtacaatc atccatgtcg
    13801 atcatgatgc acctgtagat attgcggaga atgtgacggt aggacatcag tgcatgctac
    13861 atggctgtac aatcgaaaaa ggggcgctga tcgggatggg gaccacgata ttgaatcatg
    13921 cggttattgg tgagaatagt ttgattggtg ctggatcatt agtaacagaa ggaaaagtta
    13981 ttcctcctaa tgtcttggct tttggtcgcc cagctaaagt gatccgtcct ttgacaaaag
    14041 aagaaataca aaaaaacaaa gaaaacatcc agcattatgt cgagataggt cggatgcatg
    14101 cgcaaggaaa attcaaggag caaagatagg cagtagcatc cataaaataa aaaaggatgg
    14161 tcagtttgag ttgaccatcc ttttttattt ggtttagata agagagctta aaaaagcttc
    14221 ctttgaaaca gattaattta atgaagaact gttttccatg acttcatcga tcaatccata
    14281 tgcttttgct tgttcagctg tcatatagtt gtcacgatct gtatcttttt caatcacttc
    14341 aagcggttgt ccagtgcgtt cagctaaaat tttgtttaag cgttcgcgtg tttgcaaaat
    14401 atggcgtgcc gcaatttcga tttcagttgc ttgcccttga gcgccgccaa gaggttgatg
    14461 gatcatgatc tcagcatttg gtaaagcaaa tcgtttacct tttgtaccag ctgtcagcaa
    14521 gaagcttccc attgaagcag ccatacccat aacaattgtt tggacatctg ctttgacaaa
    14581 gttcattgtg tcgtaaatag ccatacctgc tgtaactgaa ccaccaggag aattgatata
    14641 aagatagata tctttttcag aatcttgagc gtctaagaaa agcaattggg cgatgatcga
    14701 attagcaaga tcatctgtta cttggccgct taacatgatg atacggtctt tcaataaacg
    14761 tgagtagata tcgtaagctc tttcacctcg tgaagattgt tcaatgactg taggaattaa
    14821 attcataata gagttcctcc ttgatttcgt ttagaaatag tgtaccataa tttagtttct
    14881 aatacctaaa atgtagtata catttttggt caaaaaaggt caaataaaaa acttaggcaa
    14941 attagtcgca taaacccgct ttatggtatg ataagcagag aaacaaaaca aggaggaaca
    15001 acagattgat agttggtatt actggcctta tcggagtgct gacaggtgct gtagttgtct
    15061 atatcggcat gatactgcaa atgcgtcact tggaaaataa agaacagcgt agacgtgaat
    15121 tggaattgaa agtcaaagaa atcgagacgc tgaatcaatt aaataaaaag atcaacgaga
    15181 tcttgcaaaa acggacaacg ctgatggaag aatatgtcag ctttgatgca tttgatgatt
    15241 gttatatcac gattgacgat tttgcgtatc tccaatcatt ttcagcgcaa aatagttttt
    15301 atctgccaaa ttatatttta gacgagctat ttaagaatat ctcccatcga aaagtgattt
    15361 tatctccaga tgaaacaatg aagattggtg gatatgctta taaaggcgga cgcgtgattt
    15421 tagagaattt tacggatagt ctgactgaaa tgatcaatga aaaaaaagct cagttgaaga
    15481 ctatgacgcg ccagccgatt ggtttcttct cgaaagatga tgtttggagc tgatattttg
    15541 aagcattgcc gttgcacatg agagcgtggt aatgctttct ttttttctta atgatacaat
    15601 agaactgaac ggagtgaaga aagatgttac aaatctatgc ccatcgcgga agcagcggca
    15661 cccacccaga aaatacgctt cctgcatttg cagaagctgt acgagtaggt gcagatggta
    15721 tcgaactaga tgtccatcta agcaaagatg gttatttgat cgttatgcac gacgaagaag
    15781 tggaccggac gactaacggt aaagggctga ttcgagaaaa aacattggaa gaactcaaaa
    15841 aattgaatgc agggggcaaa ttcagcaaag aatttccagc agctaaagtt cctgaattga
    15901 gagaagtgtt aggactgctc ctccaaaaaa attaccgagg gatgctaatg attgaaatta
    15961 aaactgatca gtatgaatat cccggtatcg aagaaaaatt agccgaaaca ttaaaaagta
    16021 gagattggcc ttttcaacat gcatattgca gctttcattt ggattctttg gaaaaaatcg
    16081 cattactgga accggagata cagctggact tattgatgag tacttctgat aaaaagatcc
    16141 aactagcagc agaaacccct tatatagaag gagtacatcc aaaaaatact tggttgttcg
    16201 aacatccagt ccaggcagct gattttccaa aagcaattcg tgcatggacg attaacgatg
    16261 aagaagaaat ggccaaatgt caagaactca aaatagatgg aataataact gattttccgg
    16321 agaaagctct tcggtggaaa caatcaacga aaagtaggta aaatatggaa aaagtattag
    16381 tgatcgtcgg tccgacagca gtcggtaaaa cagcgctaag tgtggaactc gcacaactat
    16441 ttggaggaga gatcatcagt ggtgattcat tgcaaatcta taaaaatctg gatatcggta
    16501 cagcaaaaat caaagaagaa gagatgaata atgttccgca tcatttgatt gatgtgatcg
    16561 aacctactca acgttactca gccgttgatt ttcagcattc aggtcgacaa ttgattaccg
    16621 agattgcgaa ccgagggcac ctacctatca tagctggtgg tacgggtctc tatatccaat
    16681 cccttctata tgattatcag ttaggagcga cagaagaagc gaatcctgag attagaaaaa
    16741 agtatgaaac gttagctgaa acaattggtt cccaagcatt atggaatcag ttgaaagaaa
    16801 aagacccggc agctgctgag aaaatccact ggaacaatca acgcaaagtg ataagagctc
    16861 tggaagtttt agagacgaca ggacatagta tcacggcacc taaagaagag ccaaagcaat
    16921 tgtatgatta cctcatgatt ggattagata cgaagcgtag tattctttat gaacgaatca
    16981 atcaaagagt cgatttgatg ctagcagaag gtttagtagc agaagcgcgt aaggtgtatg
    17041 agcttggaga agtccaggcc agtcaaggta ttggatataa agaatttttc ccttattttg
    17101 aagggaaaga aacattggaa gaagcaacag aacaagtcaa actacattcg agaagatacg
    17161 cgaaacgtca gttgacgtgg tttcgtaacc gtttggatgc acagtggttt gatcttataa
    17221 gtgagccaaa tcaaaaaagt cagattgagc aaacaatcaa aaaatggctg gaggcggaat
    17281 aaaatggaag aaaaagtgat catcgtaggg gtagaaaccg aagcaaacca gcgttacttt
    17341 tcagagtcaa tggaagagct agttcagctg acgaataccg catcaggaga agtcgtattc
    17401 acaattacac aaaagagacc acaagtggat cggcaaacga ttatcggtaa agggaaatta
    17461 caagaattag ttcaacaagc ggatgcgcat gaagcggatt tgatcatctt taatcatgag
    17521 ctaactccga gacagagcca actgattacc gatgctgttg gtcttccggt aattgatcgc
    17581 gtgcagctta tcttagatat ttttgcgatg cgtgctcgtt cgaaagaagg gaagctgcag
    17641 gttgaacttg ctcagttaga atacctgctg cctcgtttag tagggcaagg gaaaacatta
    17701 tctcgtcttg gcggtgggat cggaacaaga gggccaggtg aaacaaagtt agaaacagac
    17761 cgccgacata tacggaataa aatttttgct gttaagaaag aattgaaaga agtagaagct
    17821 catcgagagc gcaatcgcca aaaaagaaaa aatagcgaga tttttcagat tggacttatt
    17881 ggctacacga atgccgggaa gtcaactatc ttgaatctat tgacacaagc ggatacttat
    17941 tcgaaagatc aattgtttgc aacgttagat ccattgacta agagatggcg ttttgctgaa
    18001 ggatttgaga ttactgtaac ggataccgtc gggttcatcc aagacctgcc tacgcaactg
    18061 atcgatgctt tccattctac attagaagaa agtcaaaata tggatctttt gctacatgta
    18121 gtcgatgcta gttctccaga tcgtatttta caggagcaga ctgtcttaaa attgatggat
    18181 gaactgaata tgaaagaaat gccgatactg actgtttaca ataaggcaga ccagatcgac
    18241 ccagcgatgt tcacgcctac gctattccca aatgtattga tttctactca gacgaaagaa
    18301 ggtaagatgg cgttgatcga agcaatcaaa cgtcagctga tggaactaat gaccccttat
    18361 acactttctg tgccaagcaa cgaaggacag aaattaagcg agttgagaag acaaacaatg
    18421 gttttggacg aagcgtacat ggaagaaaca aacgaatatg aagttaaagg gtttgcaaaa
    18481 accaattcaa aatggttaag aaaaaaccaa taaaatttta tttttttaa
//
LOCUS       DAJSKS010000046        18487 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJSKS010000046 DAJSKS010000000
VERSION     DAJSKS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.047"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..819)
                     /locus_tag="ON396_001351"
     CDS             complement(<1..819)
                     /locus_tag="ON396_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR3113444.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKN"
     gene            complement(792..1472)
                     /locus_tag="ON396_001352"
     CDS             complement(792..1472)
                     /locus_tag="ON396_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR3113445.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1495..2433)
                     /locus_tag="ON396_001353"
     CDS             complement(1495..2433)
                     /locus_tag="ON396_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCR3113446.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2678..3541)
                     /locus_tag="ON396_001354"
     CDS             complement(2678..3541)
                     /locus_tag="ON396_001354"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR3113447.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(3683..6256)
                     /gene="mprF"
                     /locus_tag="ON396_001355"
     CDS             complement(3683..6256)
                     /gene="mprF"
                     /locus_tag="ON396_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCR3113448.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6397..7203
                     /gene="proC"
                     /locus_tag="ON396_001356"
     CDS             6397..7203
                     /gene="proC"
                     /locus_tag="ON396_001356"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR3113449.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7282..8838)
                     /gene="rny"
                     /locus_tag="ON396_001357"
     CDS             complement(7282..8838)
                     /gene="rny"
                     /locus_tag="ON396_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCR3113450.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9249..10298)
                     /gene="recA"
                     /locus_tag="ON396_001358"
     CDS             complement(9249..10298)
                     /gene="recA"
                     /locus_tag="ON396_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR3113451.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10407..11648)
                     /locus_tag="ON396_001359"
     CDS             complement(10407..11648)
                     /locus_tag="ON396_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HCR3113452.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11888..12466)
                     /gene="pgsA"
                     /locus_tag="ON396_001360"
     CDS             complement(11888..12466)
                     /gene="pgsA"
                     /locus_tag="ON396_001360"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR3113453.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12675..13598)
                     /locus_tag="ON396_001361"
     CDS             complement(12675..13598)
                     /locus_tag="ON396_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3113454.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14337..15623)
                     /locus_tag="ON396_001362"
     CDS             complement(14337..15623)
                     /locus_tag="ON396_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR3113455.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15616..16884)
                     /locus_tag="ON396_001363"
     CDS             complement(15616..16884)
                     /locus_tag="ON396_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR3113456.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17136..17597
                     /gene="mscL"
                     /locus_tag="ON396_001364"
     CDS             17136..17597
                     /gene="mscL"
                     /locus_tag="ON396_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR3113457.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17656..18102)
                     /locus_tag="ON396_001365"
     CDS             complement(17656..18102)
                     /locus_tag="ON396_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3113458.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18195..18440)
                     /locus_tag="ON396_001366"
     CDS             complement(18195..18440)
                     /locus_tag="ON396_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCR3113459.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         5277 a         4046 c         3229 g         5935 t
ORIGIN      
        1 attttttctt ctgaaggcaa ccaatattcg tttgatgata tttacaatca ggaacaaagc
       61 aataaagact aatgtgattg ttgctcctgg aggtgtatct agataaaacg agctagttag
      121 tcctgacagc atccctagaa atcctacaca aacactgata gcaatcacga cattgaagct
      181 tttgcctaat cgcataccga ttgctgcggg aagaaccatg attgcggata tcaaaagtgc
      241 accggcaatc gggatcatca cagcaatagc cacaccagta atcacattga acatcatcga
      301 catcaaatga acgggtagcc catccacgtg ggcggtatct tcatcaaaag tcagaacata
      361 catcggccgt ttgaataaaa agaacaatat accgatggca acgaataaga ttcctaaaaa
      421 gatgacttgc tcccatgtaa tggtcacgat cgaaccaaac agataggatt ggatacttgt
      481 tgctgaattc cctccgctca aattcatcaa tactaacgct agcgccaaac caccagccat
      541 taaaatggca atggagattt ctgaataggt tcggtagatc gttcgcagat actccaatat
      601 cacagctgct aatacaacga ctagcatggt cgtaagtgta ggattaaggt tcaaaaagaa
      661 tcctaacgct acccctgcta aagagacatg agaaagcgta tcagccatca gcgattgtct
      721 tctgatcact aaaaacacac ctagcatcgg ggcgattccg gcaatgaaga tagcggcaag
      781 aaacgctctt ctcatgaatt catatgaaag caacgccatt gagaatcctc ctttctcacc
      841 agtcggatct gtctgtccgc atactgtttg atatcttcat gatcatgggt gatcattaga
      901 actgatttcc catggacatg cgcactatgt cggagaagac gataaaattc atttctggat
      961 tcttcatcca tacctgttgt tggttcatca agaacaaata agtcagggtc agtagcaaaa
     1021 atacgcgcta aactgattcg ctgcttttgt ccgccagaca attcacctat tcgccgattt
     1081 cgcatatccc acatccctac agcttctaaa gcacgctgta catgttgatg atctttttca
     1141 gagagacgct tgaaccagcg gcctcttgga tatcttcctg aacggactaa ttcgatcacc
     1201 gtactaggaa aacctgcatt gaaagatgca acttgttgag gaatataacc gatacttaat
     1261 ttttttcctt cttgattttt ctcagcaatc tttatcattc cttttgaagg tttcagtaat
     1321 cctaacgatg ctttcaccaa ggttgacttg gctgctccat tttctccagt taaaatgaca
     1381 aattcacccg catctacatg ataagagaca tcttctagta caggttcatc atcatagtaa
     1441 aaggtcaaat cagatatttc gatataacgc accttacaac ctccattcta aaacttaatt
     1501 gatactttct tttaaagctt tcagattttt ttccatgaca gaaatataat tttcgcctgc
     1561 tttgatttgt tcattagtca agctttctaa aggattcaac acttctagct tcactcctgt
     1621 ttcattcgac aatgtctcag ctaccttcga agaggcattt tcttcaaaat aaatcacttt
     1681 gatgttgttt ttcttcacaa attcttttag ctcagctaag cgacttggtg tgggttcctg
     1741 atctggtgaa acgcccgaaa tcgctacttg ttttaagtcg tattgattcg ctaaataagc
     1801 aaaagcagca tgctgagtga caaatgtgcg gtttgttgca tcttttagct catcggagta
     1861 tttttgatcc agtgcttcaa gctttttgat ataagcatcg gcattcttcg taaaagtttc
     1921 ctttttatct ggatattttt cgcctaattc ttcagcaatc gttctgactt cttttatagc
     1981 taataccgga tcgagccaaa catgtggatc gtattcatgt gaatgaccat gcccttcttc
     2041 tgattcttca tgttcgtctg cttcttgcga ttctaaacgg tcgattcctt tagctgcttc
     2101 gatcatcaag gtctgtttgc tatccactga atcctttaac gaatccacaa aaaattccat
     2161 gtctgaacta ttatacacga aaacatctgc atcggaaatt tctgctcttt cttttgcact
     2221 tggttcgaaa tcatgcggct ccgttccagc agggatcaaa agtttcacgt ttccttcatc
     2281 ccctacgatc tcttttgtaa aatcatacat cggataaaaa gtcgtaacga tcgtcaaatc
     2341 ctcttttttg tctgcttcct tgttcgtatt tccacatgct gcaaacagta aagcacctat
     2401 cattaccgta gctgtcaaaa gatatttttt catttatatg ctcctttcaa tgaacaaatc
     2461 ggaattttac gatttattaa cgtgaactaa tgtatcaaaa taaaaaaaca cagtcaatag
     2521 aaatccatca atttctgatt taagcatgta tattcttgat taaatcgtaa aatggatgag
     2581 ttgctgctca tatctagcca tttcactaca ataaaaaact gaagaatacc attttaggat
     2641 tcctcagttt ttcatcacct attttacttc ttatatttta ttgcagggtt cgtactaaat
     2701 aaacttcctc acaaaaacct tttaacccat tatatactct ctgcgctctt gaataatgtt
     2761 gacacaaggc aaaaacggtt ggtccgcttc cactcatcaa tgcagcatct gctccgtact
     2821 tgatcatacg ctctttgatt tgatggatga ctggatgccg ctttcccgtt atatcttcta
     2881 aactgtttcc catttttttg atcatctgct gataatcttg ctctcggatg gcttcagcaa
     2941 gtccgtggat gtcttgatgt tggatggctt gcgtatctac ttctctaaac actgtacgtg
     3001 tagagacact cattcttggt ttgaccaaca caacccagca ctgtggcata gaaggtaata
     3061 cttggatttc ttcccctttg ccaccgacaa atgccgtact tccgtagaca cagtaaggaa
     3121 catctgtacc gacttccaaa ccgatttctg ccagttcttc taacgaacag cccagattcc
     3181 ataattggtt gatccctctt agagcagctg cacagtctgt acttcctccg cctaatccag
     3241 ctgctactgg aatattcttc ttaacaaaga tcttgacacc ggttttgata ttgtatttat
     3301 tttttagcaa taaagcagct tgataaacat tgttccgttc atctaccggt aaaaatgctt
     3361 tatttgtttc tacaatgatt ttgtcttctg ttatttcttc aaaaaataga tgatctgcca
     3421 agtctacgct cgacatcacc atttccaact catgataccc atcttgccgt ctatataata
     3481 catccagtcc taaatttatc ttggctggcg ctttttcaat caattccatc ctctcaccca
     3541 ttcgaaaagt ctaagtcgca agtattctat catagggaaa aataaaagta tagcttatgt
     3601 ctgcctagtg gttgattttg tcatcgtttc aacagctttc acagaatttt ctcttcataa
     3661 tccaaacaag ttctagtgat tcttataatt ctgtgagcac ttcttctgcc aaactcggaa
     3721 catttccttt ttcttttcct atcagactga gtagctgcat ggtagcaaat ccgaaattac
     3781 ttcctttcct ataagcaaaa taacgtcctt cccaatgatc gacatatttt aatttctttt
     3841 cataagcaga ctggaaggaa tagaaaaaat taccataatt atatacgata tgcatgactt
     3901 tttcttgaaa gaaagaaaaa tctgtttctc caacattagc taatggagct aagcctagat
     3961 tggcttccga gtagcccatt ttctcgtata caataaacag attggctaac agatagtcag
     4021 ctaattcctc agattcttta ggtaagaccc gcaacaaatc atatcccgcc tgcttcttcg
     4081 tcatcggctg agcggtgatg aagccaacga ctttttgatc tttacggaca ataccgacac
     4141 cgctagataa tacatatgat ttatcaaatc gtcccacaga gaagtatttt tctcgtgcac
     4201 cttgaagcca tttttgtgaa acttcattta cagcttgatg taattcttcc ggcagttgat
     4261 cgtaccaagt aaaatggtat ccttctttca tcaaacgttg atattcaagt tgctgactca
     4321 gccgagacaa gtcttctgtt gctagatcaa caatccctgc ttcacctact ttcgtgaagt
     4381 gaaaacccaa ttcatgaagc atcactacgt agctttctga tatcttataa aacgcaagct
     4441 gatagcctaa acaatctgct tgtttcataa actgattcgt tgctgcccgc atcattttag
     4501 gattgccaac tggatctcct aaaacaaagc atttgtttcc tttgatctga taaccaaaaa
     4561 cgacttgatc ttggtcgtct tcttggtaat aaaaataact gtaacctgac aagcgaagct
     4621 gatggcttgt agctgtgcct ccgtatgttt ccaataaacg gtcaaaacgg tctttttcgt
     4681 actcactgcc aagcttcgta gtatcttcag ctaaatattg ataaagcgcc actaaagcta
     4741 gcaatgagat gcccaaacca ataagccctg agaaccacac atcatctgaa ggaaacaaaa
     4801 taaaatgatt tcctgttatt cgaccgacta aaaagcgtcc gctgtgatag cctgcaattg
     4861 cgtaaacgat aaacaaaaag ccgaacaagc tagcatcaaa gattatagct ccccaagaat
     4921 aaacgaattt tgttcgataa aattcttttc tggataacca cacagctaac agaatcaaga
     4981 catataccaa tatcaactgc caagataacg tacgtgtgat tgtatttaaa ataccaaaag
     5041 atagtaaagc aatcgtaggc agaaatgcct ttttgacttt tactgaaagt gctctagcaa
     5101 ggccgagtaa taaaaagcca accaatagat tcactgtctg atctaaaaaa ttgaatgaga
     5161 acggcaataa tacttgaaat agccgactga ttgcagacag attagtgatt gttgatagaa
     5221 gtaccatcat gatccccgca aaatagacag cacttacgag cacgatatgc gcaacctttt
     5281 ggaaaaacag tcgcggaatg ttatctaaaa atcgattgat tttgactcct gtatgtgtga
     5341 tgaacaatag aagtcccgtc ataaaaggta gaacataata aaacaaccga taatataata
     5401 gccaaactac tgctacatct tgccgaacag acaattgact cattcctaag atcatcaaaa
     5461 cgtcaaaagt ccccattcca cctgggacca tcgtcagcat tcctatcaat gtagcaatca
     5521 tgaacatagg atagatcgaa agcatcgaaa tatctattct catcaacttg ccgatgatca
     5581 aaaataccgc aagggctccc gtccattgtc caaaggatgc cagtagcaga ccaagcaagc
     5641 cttttggata aaactcgaag aacaatgtct ttctctttag ataagcaaaa aagaaaatga
     5701 ctggtgctaa aaaactaccg ccgaaaagcc agatccaata gcttcgaaaa atactttctg
     5761 actgaataaa aaagacatca ataaatgtaa gaaacgacca tacagacaaa ccagtcagca
     5821 taaatagtgc gacttttgat acagtggcta aaaccatttt tctatttgtc tgctttccat
     5881 aaaaactagc ccgcagactt gctcctacca caccaccaaa accggcgaga ttgttgatcg
     5941 tgtttgttac ccacgcagca agaagaaact cttttctttc catttttggt tttccttgtt
     6001 tttctaagac tctgatcgtc acccaatcat acaacaacat cggagtgaca cccagcagtc
     6061 cagccagaat catgagcagg atcgtcatgc ggctttgttg tcccatcctt tgccaaatat
     6121 cttgccaact catgccatta gcaatatttg cgacttgatt tgccacaaag actaacacgg
     6181 agccgaaaaa aatcagtttt aaaatcagac tatgttcctt tatccattgc agcatcaagt
     6241 atcgtttatt tttcacattt tcccattcct aacttgtata cttttgtgtc ataccccaat
     6301 gttatcttat ttattttaat ttttccactt tagacctgtt ctttgcttgc gatgatagaa
     6361 aaagaaagat atcctaatgc taaaggagag gataacatga aagtaggatt tatcggcaca
     6421 gggaatatgg cacaagctat tattgatggt ttattaaaaa aaggaacagt ctcgaaagaa
     6481 gaggtgttcg tttccagtgg acattttgaa aatgcacaaa aatttgctgc tgaaactggc
     6541 gtccatgcat gcaaaagtaa tcaggaagtt gcatcacaat cagatatcgt tcttttagcc
     6601 gtgaaaccaa aaatcattga aaaagtattg gaagaactaa gagaatatag ccagaaagtc
     6661 ttattcattt ctattgcttc aggaaaatca ttaatagatt tagaaacagc gcttgaagat
     6721 cctcaggcct ccatcgtacg tgtgatgccg aatgtcaatg tcagcgtagg agcaggcgtt
     6781 tctgcgattt gcaaaaatga agctgccacg gaggaacagc ttcagcaagc tacgatgatt
     6841 tttgaagcaa tcggcagcgt ctatccttta gcagaatcag atttcagtac attcattggc
     6901 cttgctggca gctcacccgc attcgtttac atgctgatcg atgcgatggg acggactggt
     6961 gtactgaatg gtttgtccaa acagacagcc actgagattg ctgcaaaagc aattatgggt
     7021 tcttgtcaaa aattcctttc aagtgataaa aatccatggg aacttgtaga ccaagtttct
     7081 tctcctggcg gtacaactgt tgcaggtgta gttgccttag aagaagcagg attcatccca
     7141 gcaatcatca aaggaatcaa tacgacgatc gagaaagatc ttgagatgtt gaataaaaaa
     7201 taatgatagc aatgtttttt cgtgccttac aaaaaaagaa gagctgtggc ttttgccgcg
     7261 gctcttcttt tgtatttttt attatttagc ataatcagtt gcacgagttt cccgaataac
     7321 gattactttg atgtgtccag gataatccag ttcttcttcg attcgtttac gaatatcacg
     7381 tactaaacga acagcgtcta aatccgagat ttcttctggc ttgaccatta cccgaacttc
     7441 gcgtccagct tgaactgcgt aacttgattc tacaccggcg aagctgttcg aaatattttc
     7501 cagacttctt aaacgacgga tgtagttttc taatgattca cttcgcgcac caggtcttgc
     7561 tgctgagagc gcatctgctg cagctacaag gacagagata acagaagtag cttccacatc
     7621 tccatgatga gaagcgatcg cgttgattac tactgggttt tctttgtact tggcagctaa
     7681 ttccgcaccg atctcaacgt gagatccttc aatctcatgg tcaagtgctt tcccaatatc
     7741 atggagcaac ccggcacgtt ttgctaattg gatatcttcg ccaagctctg ctgctaagat
     7801 accagctaat tttgcaacct cgaccgaatg cttcaagacg ttttgtccat aactagttct
     7861 gaatcgcaga cgtccgatga tcttgattag atctggatga agtgtatgag caccgacttc
     7921 aaacgctgcc tcttccccgt attcgcgaat acgttcatcc atttctttac gagatttctc
     7981 gaccatttct tcgatacgcg ctggatggat tcgtccatct tgtatcagtt tttccaaggt
     8041 catccgtgcg atttctcttc ggattggatc gaatccggaa agaacaactg cttccggtgt
     8101 atcatcgatg attaaatcga taccagtcag cgtctcaagc gttcgaatat tacgaccttc
     8161 tcgtccaatg atacgacctt tcatttcatc attcggaagg gtaacgactg aaacagtcgt
     8221 ttctgaaact tgatctgctg cgcagcgttg aatcgctaat gataataagt ttttcgcttt
     8281 gcgatctgct tcttctttgg cacgctgttc agattctttt accattagag ttaactcatg
     8341 attcagctct tcttcagtcg atttcatgat gacatctcgt gcgtcctctt ttgataaacc
     8401 agcaattttt tctagttctt tttgttgatc ttcaatcatt ttttcaacgt ctttttctcg
     8461 ctcttcaatt aattgctgtt ttgaactaag tgcttcttct ttactttcaa gtgagttctc
     8521 gcgtttttct aacgagtcat ctttacgatt cagcgtttgt tcgcgttgaa tcaaacgatt
     8581 ctcttgagat tttaattcta acttactttc tctcagctca ctttcaactt cagaacggta
     8641 tttctgattt tcttccttag cttctaacaa tgcttctttt tttagagttt ctgcttcttt
     8701 ttttgcacta tcgatgattc cagcagctga tgattgtgca ccagctattt ctttctcgtg
     8761 acgtgacttt gcgaccataa accctaaacc aagaccgaca attaaaccga tgatagcgag
     8821 gagaatgttg aataccataa aatccacctc catactatct ttttcatttt tatcatctat
     8881 acagcttcta tgataaatta ttctaaactc gtcaatatgc atcttcgcat atatgtgtac
     8941 aaaaaacatg cacaacttta ttctaatgtt tgtaccccgt agtgtcaact ttaaataaac
     9001 aaagaaacgg ctttctactt attcattaaa cgatggtttt taaaataaat gacactagtt
     9061 tcatataggt aaacctttaa attgagaaac ttttctatta aattattctt tgtagatgaa
     9121 cagatatttt tattttatgt acccactcgt ataattaaat aactcacgat ttctttattc
     9181 aataaaaaaa cggcaggtca aggagagtac ctactcttca gaccagccgt ttttgtttat
     9241 cataattttt attcttctac taaaggcaat tcttcttgtc ctttttcatc ttctgggaca
     9301 gcgacttctt caccaatacc ataagctgct ctaactagag cagaaacttc tgccatcatt
     9361 tcaggatgat tagccatata gattttagcg ttttcccgtc cttgaccaat ccgatcttct
     9421 ttataagaat accaagcacc gcttttatca acaatgtctt tttctactgc catgtctaga
     9481 agttctcctt cttgtgagat ccctaaacca tacatgacat ctacttcagc gattttgaaa
     9541 ggaggtgcta ctttgttttt tacgactttg atctttgtac ggttaccaac aatatctgta
     9601 ccttgtttca attgttctgc acgacgtact tccaaacgga tcgttgcgta gaatttcaat
     9661 gcgcgtcctc caggagtgat ttctggattc ccaaacatca cgcctacttt ttcacggatt
     9721 tggttgatga aaatagcgat tgtttttgtt ttgttgatcg aaccagagag tttacgcaat
     9781 gcttgagaca tcaaacgtgc ttgtaacccg acatgtgagt cacccatttc gccgtcgatt
     9841 tctgctcgtg gaactaaagc agcaactgaa tcgactacta cgatatctac agccccactt
     9901 gatactaaag catcagcgat ctctagacct tgttctcctg tgtcaggctg tgaaagaagt
     9961 agttcatcga tattcacacc taatttttgt gcatattgcg gatctaacgc atgctcagca
    10021 tcaatgaaag cggccgttcc gccatttttt tgtacttctg caatagcgtg tagtgcaact
    10081 gttgttttac ctgaactttc aggtccatat acttcgatga tacgtccacg ggggtaaccg
    10141 ccgacaccta aagcgacatc taacgcaaga gagccacttg gaattgtaga gatttgttgg
    10201 tcgatttttt cgcccaattt cataatagag cctttaccat agctcttttc aatttttttc
    10261 agtgcagcat ctaaggctgc tttacgatca tctgccaata cgataatcct ccttatattt
    10321 acaaatcaga gcaacatttc attatcctta tcatatcttt tttagtgaaa aaaagcaaga
    10381 aaaaaccgaa caaatattcg catttatcat tttcttaaaa gctgttttct caaaagatca
    10441 aatccttgca taaccgcatt atatctgata gcagcacgat ctctggcaaa gtgatatttt
    10501 tccgaaacca cgccgtcttt agatgaaata gcgatccata ctgttcctgc agattgacct
    10561 tctaacgtgt ccggtccagc cactccggtg aaagctacac tgtaatctgt tcttgctatc
    10621 tcacgtgctt gaatggccat ctgctccaca cattctttgc tgactgttcc atattctgct
    10681 aatagctccg gatcaatctc cagaaaacca gcttttgttt ggagcgagta ggtaacgaat
    10741 ccgcctggaa atacttcaga tacaccagca atgtcgccta atgcggcttg gaaggcgccg
    10801 gctgtcaaac tttccgctgc tgttacagtc tgcttgtttt ctttcagtag ttccactacg
    10861 acttttgcta gcgaattatc atcgccgtat ccataaaaat attcccctac tcgttcttgg
    10921 attttttctt ctaatgctaa aagagcttga ttccctgcct gaacatcatt tgttttcact
    10981 gtcagtcgta atgtcacttc atttggtttg gcatatggtg cgatcgttgg attgatctgt
    11041 gtctcaatca gatcttttag ctcagtgacc agtcgcgatt caccaatccc ataaaagcgc
    11101 aaaactttag aaattagttt ttcttcagaa ggaaaacgtt cctctagtaa aggacgtact
    11161 tgttccacaa acattggttt caactcatta ggcggaccgg gaagaaggat atacgcatgt
    11221 gtatctgttt gataaaaagt ccctaaagca agccctgtac gatttggtag aggaaccccg
    11281 ccttcgataa tctgggactg ttgcagattg tttttggtca tttttcgatg ggttgtttca
    11341 aaatatgcca ataatttttt gtatccttcc gtattttgta tgagagactt accgaggtga
    11401 gccgcagttg cttcttttgt caggtcatct tctgtcggtc ctaatccgcc acataagagt
    11461 atgagttcac ttcttgtttc agctagagca atcaactctt ctaaacgctg ctgattatct
    11521 cctacaactg tttgataata aacttcgata cctagatcag ctagctgttc tgacaagaaa
    11581 gtggcattcg tattcacgac ttgtcctagt aaaagttctg ttccgacagc tattatttct
    11641 gctttcatta cttcccgctc ccttatatag tatacgctgt atttttatat ttttttattt
    11701 aaaaagaggg caaggagctg tgacaaaaat cttgtcacag ctccttgttc caaataaaca
    11761 gtgttctcct gccaaaacct cggtcataag tcacaatata cataaaacat gaaaagctgt
    11821 tttttgaaat tatcccttgt gactcagagt tagacggcag gctcacaacc tctccactaa
    11881 acgatactta cattgatcct ttgaacacac cgctgttttt gatgaagtaa tccacacctg
    11941 aatagatagt aaagatcaga caagcataaa gcatgatttg atctactgga ataccgatca
    12001 ggctgaatgg gatattattg ataaacagca agataatagc aaccatttgt gtagccgtct
    12061 tgacttttcc cggccaagct gcagccatga cttctccatg ttcgacaagt agtaaacgca
    12121 agcctgtgac ggctaattca cgacagacaa tgatcgctac tacccaacca ggggcctttc
    12181 cttggtcaac caacatgata aaagcagtca taactagcat tttatctgcc aacgggtccg
    12241 caaacttgcc aaaattagtg acaaggcctc gcgcacgggc aatttttcca tctaaccagt
    12301 ctgtgatact agctaaagca aaaataactg cacctactag ctgtgtgaca gccagtgtgg
    12361 tgtctcctac gttaaattgt ccccaatcta aaggtaaacc tacaaccaaa atgaaaatcg
    12421 ggatcataca aattctaagt acagttaatt tatttggtaa attcacacta tcgactcctt
    12481 tcttccctat tctacgtgac tatcagcgaa gtttccaccg atattattta cttttccaaa
    12541 tgaatttatt caaaggagta tttttattga aattgatctt tttgaattaa cgaagaagga
    12601 ccgcgacaca gatttgtcac gatccttctt ttttatacct acaagtaaac agatgcgtaa
    12661 actaatttat acgatcattc tgcgtattga agagtaaggt tcacattttt tggactctga
    12721 ttgttttccc ctggattaac tggtacggat tctccattcg ctttgatcgt tgcattgctt
    12781 gctgcaccaa tcgtgatggt cgcatttgtt gctgtttccg gtaacacggt actctgtgtt
    12841 tcatttgctg ccagtgtccc ttgataaaca taggcattgt ctactagcac acctaaccat
    12901 acacgattga cagcagtaaa ttcaagggtt acaggcttgg tagcgtctgt gatatttact
    12961 gcgatggcac tgccagaatc ttctcctgta gctattgcca ttttcttttc ttcacttgat
    13021 tttgttgttg attctactgt agaagactct ttggtctgcg tgctttgctc tgaggaagag
    13081 gactcctcga ctgatccttc tactttaagc gaagaagctg tgtctccgat tattggatca
    13141 gcttgacgat cttgccaagt catatagccc acgacaacga caatcgccaa agcaaccaat
    13201 cctaataaaa tgacaggaag acttgtccaa aagcgacttg gatgcgattc ttcctgatgc
    13261 attgcttttc tagaagtact tactgtttca ggctgcggac gttcagggac cgatcctgtc
    13321 aatttgcttt tcccatcaaa aacatcgact aaatggtctc catcttctcc tacagcttca
    13381 gcgtattgcc gaataaatgc ccgcacgtaa aatttaccag gcaattggtc aaaatcatca
    13441 ttgtcgattg cttccaaata acgtttttga atttttgtaa tctgctgtaa ttcatctaaa
    13501 gatatttttt tattcaggcg tgcttgccgt aatttttctc cgatggtttc aaaagccact
    13561 cgttcatctt ctccagtctg tttgattctc cgaagcatct gctgttgcaa cgaaagacgt
    13621 tatgtttggg tgctgtgaca aaagtcatgt cacagctcct tttttccgaa taaacattgt
    13681 tctcctgcca actcctcggt cacaagtcac aatatccaat aaatataaga agctgttttt
    13741 tgaacttgtt ccttgtgact cggagttaaa cggcaggctc acgacctctt tcttaaacgg
    13801 tgttcaatca gctgttcctc ggtattagct caaaaaccgg caaaatataa ggagatattt
    13861 ttccgtatgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttccttaaac
    13921 ggtgttcaaa agcttgcatc tgcggtaata agtccaacca agttaaaaac attaagaagc
    13981 tgtttttact tggctgttct tattacttga tgcagggcag ctttttcaca acctcttggt
    14041 acacggtgtt ccagaagtag cttcttcggc aataagccga aatatcacaa aaatttgaga
    14101 agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctctt
    14161 tgattacttc gtttgatttt attttaatat aagttgatta aaagagagca tgatttgttt
    14221 tattttctca ctaatttttc caacacaact aagaatacca taaaagctga ttttggagga
    14281 ggttcctact acaagtctaa aacgaaattt aagacttttc ctcataaaat actggtttat
    14341 tttggtctca tgtaaaactc gctgaaggcc tcttcactta taaatgcttc cccagctgcc
    14401 agtacgtctt tcatttggat cgaatcgatg atttcaggaa gatcaaataa agttcgatcg
    14461 ccaaataaac tttgcgtaaa ttgattggca atatattcga tcgaatttaa tgattggaaa
    14521 tattgtccaa gcattttctt cttcagcaaa tcgagatttg tttctgacac ttccggatca
    14581 tcagcgaatc caagaatgat ttctttgact ttttctgctg ctttctctgg ttcgtccgta
    14641 tctccgctga agtccgcaaa gtgaaattca cgatccaagc tgaattcaaa accaaagctg
    14701 tcatcgataa ttccttgatt gtacatcgct agatagtttc gagaagtatt gcctaaaatc
    14761 atttggaaca acagattgat tgctgtttta tagcgtaaca gttctgttcc ttcttgcggc
    14821 aatgtgtcta atcctttaat accaagtaca aatttatctc gtgtaatggc tgcttccaat
    14881 gcactttgtt tgatgatttc ttttgtgttt tcagggaaat aacgtacaat ttcttgcttt
    14941 ggagggaaat tttttgcttc ttggttttcc cgaataagtt tcatcaattt ctcaggctcc
    15001 atcttaccaa ctacaaataa aaccatattg cttggctgat aaaacgtccg atagcacgta
    15061 tacaagtctt gagctgtgat tttgtcgata ctttcaaccg tgcccgcaat atcgatatgc
    15121 aatgggtgtg taggatacaa gttatttagt atgccaaaaa acatccgcca gttagggtcg
    15181 tcttcataca tttggatttc ctgaccgatg attccttttt ccttgtttac agtttcttct
    15241 gtgaagtaag gtgcttgtac aaaatcgatc agagtcgtca gattcttttc tacctgatcc
    15301 gtcgtagaaa acagatagct ggttttcgta aaacttgtga aagcattggc ggaagctcct
    15361 tgtttcccaa ataattgaaa gacatcacca tcttcttttt caaacaactt atgttctaaa
    15421 aaatgggcaa ttccatctgg aactttcacc ttttcttttt caccataggg aataaattcg
    15481 ttatcgatcg aaccataatt cgtactgaac aaaccatatg tcttgtgata gtcattttta
    15541 ggcaacaaat acacggtcaa accgtttggc agtacttcat gataaagtgt ttcattgatt
    15601 tgctcatatt ctgttttatt catttgcatt tcctcctgct aaaaagaaga ttgcctgcaa
    15661 ttcgatttgc tcagctactt gttgtatctc tttgatcgtt acgctctcca tacgtttcaa
    15721 ccattcttca tcagataatt ttgtttccgg cagccagctg tccaaaaaag aagcttcgat
    15781 tgccgcttgc ggagagtcta gagaaagcag atattggttt ctcagcattg cttttgtctg
    15841 agacagttcc aactctgtta tttcaccatt tcgcaaactt tctaattgct cgtgaatcag
    15901 atgaagtact tgatttcggt tcttttcatc gataccggtt tggacactca tgaatcctcg
    15961 gaacgtatcc acgctactag aggcataata agctagactt tctttctctc tgacattcat
    16021 gaaaagttta gagtgaggaa aaccgccaaa taaaccgtta aagaccatta gcgcaaaacg
    16081 ttctggttca tcgtaataca catttgtctg ataagctagg ttcagctttg cttgaacgat
    16141 tggctcacgt acttgtcgtt ccttcacgat attcacttga ggttgggtat aaaacagatc
    16201 tggataaact gtttcccgcg actcaaatgg taattgccca actgcttcag ctgctttgtc
    16261 cggatcaaca tcgccgacaa caaaaatgtc gatttgatca tttgccatca tttcttgata
    16321 gtaggacgct agcgaactag cagtgatctt gtccaaatct tcgactacgc ccaaccctgg
    16381 gatcttttgt tccgggctgt tttcaaaata aagttcttgc aaggcatagg aagcaaaagt
    16441 ttgtttatct tccttcaaac tttccaaata tagacgcaga ttgttctttt ccaagtcaaa
    16501 cgtcaactga tcaaactgat gatttttaat atttggataa aataaaacct cttttaaaaa
    16561 atctaccgct tgagaaaaaa ggtttggatc attgacatat tcaccattca caaaactgat
    16621 ccctgcgttt agccaatgta aatttccttt ttttctaact gataatccaa agcttgcgcc
    16681 ataaagctct gcaagttgcg cacttaactt tgtctgatcc gaataattca aactatttgt
    16741 ttccaacatg ctgctgagaa gtgaacgttt cgtgatgact tcttgttgca gacgcgcagt
    16801 aaaacggata aaaatccgga tcgttttgta ttgttttgtt ggtaagacat gaagattcac
    16861 gccttctgcc aattgataca ccatatttat atgctccttt tcacagttga ttgcccgctt
    16921 actcttttaa tgggtcaacc tgcctttttt acttattcct ctgcttgaaa tttataagtt
    16981 caggcaattc gccgtttttt ctaaatgatg ttacacttta tagtatagca tagatgtttt
    17041 ttatcaaaag aaacagacgg ctaaaagcgt atattgtttt tttatttttt atttttcgat
    17101 atactaaaga caaataaaag aagggagggt cattcatgat caaagaattc aaagagttca
    17161 tcatgcgtgg agatgtcttg gatcttgctg tcggcgttgt tattggtagt gcgtttacaa
    17221 gcatcgtaaa ccaagtggtg caaggcttga tcacaccact aataggttgg tttgtcgcaa
    17281 tgatcacagg tacttctgat ttagaaggag cactttcaat tcttgactgg tcgccgacaa
    17341 aaggcgtcac atttgcgttt ggtaacgtta ttagtgctat cattacattt ctgatcactg
    17401 gttttgtctt attcttagtt gtcaaagcgg caaaccgtgc gaagaattta cgctccaaag
    17461 aagagcaagt ggaagtggaa gaaactccta ctgcagaaga ttatcttagt gacatacgcg
    17521 atcttcttgc caaacaagtg gcagaagaca atacacatcc agtagagcaa acaaatcttt
    17581 ccgatacttc taaataataa agaatctaaa aaaaagaaag ccgattttca gtgtcggctt
    17641 tcttttttta gattcttaac ttgctacttg ttcctttttc atgtcgatat gtggaatatc
    17701 atcttccaaa tacacatctg agattgcctt gaatccaaac gactcataaa aagcttgtag
    17761 gtattcttgt gcgccgatca cgattggttt ttctggaaaa tttttctgga tcatgttaat
    17821 cgtttcttga acaatctttc ttcctaaacc atctttacgg ttgtcctgat gaacaaggac
    17881 acgtccaaaa tggatactct ccgcttcttc atatactcgc gtgtaggcaa ggatcttccc
    17941 atcagtatct tgaaaaaacg tatgccaagc ttctccatca atgtcatcaa cttcttgata
    18001 aggacactgt tgttccacaa caaaaactgc gattcgttct ttgacgatcg cgtaaaactc
    18061 tgctgttgac aattcttcaa atttctttac ctgatattcc actaagcaaa cgccccctgc
    18121 ttttttattc ttagcatacc gattcgcaca caaaaaagca aacgaacctt acgaatctca
    18181 ggctcgtttg ctttttaaac tgcttcagtt aaaaattcga tctcaactgt ccgagtaagt
    18241 acatctgaat agctgtaaga tactcgttca aaagaatttt cttcaggatc caaatcaaca
    18301 acgaatactg aaggataggt ttctgttaaa acgccgcggc gttctgtttg acgctttctt
    18361 cctgtctgag caaccaacat gattgggctg ccgatacgac cttctaaatc tttcttgatt
    18421 gaagctaaag ttgttggcat acagttcacc tctactatca actataccac atatttttaa
    18481 aattttc
//
LOCUS       DAJSKS010000047        18451 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJSKS010000047 DAJSKS010000000
VERSION     DAJSKS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.048"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(198..1529)
                     /locus_tag="ON396_001367"
     CDS             complement(198..1529)
                     /locus_tag="ON396_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3113460.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(1678..2391)
                     /locus_tag="ON396_001368"
     CDS             complement(1678..2391)
                     /locus_tag="ON396_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3113461.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            2566..3990
                     /locus_tag="ON396_001369"
     CDS             2566..3990
                     /locus_tag="ON396_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3113462.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            4289..4996
                     /locus_tag="ON396_001370"
     CDS             4289..4996
                     /locus_tag="ON396_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCR3113463.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            4983..5306
                     /locus_tag="ON396_001371"
     CDS             4983..5306
                     /locus_tag="ON396_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCR3113464.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(5395..6432)
                     /locus_tag="ON396_001372"
     CDS             complement(5395..6432)
                     /locus_tag="ON396_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR3113465.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(6753..7736)
                     /locus_tag="ON396_001373"
     CDS             complement(6753..7736)
                     /locus_tag="ON396_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCR3113466.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(8151..8921)
                     /locus_tag="ON396_001374"
     CDS             complement(8151..8921)
                     /locus_tag="ON396_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3113467.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(8914..9849)
                     /locus_tag="ON396_001375"
     CDS             complement(8914..9849)
                     /locus_tag="ON396_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3113468.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(10165..11814)
                     /locus_tag="ON396_001376"
     CDS             complement(10165..11814)
                     /locus_tag="ON396_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR3113469.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(11931..14534)
                     /locus_tag="ON396_001377"
     CDS             complement(11931..14534)
                     /locus_tag="ON396_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR3113470.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(14546..15595)
                     /locus_tag="ON396_001378"
     CDS             complement(14546..15595)
                     /locus_tag="ON396_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR3113471.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(15621..15935)
                     /locus_tag="ON396_001379"
     CDS             complement(15621..15935)
                     /locus_tag="ON396_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCR3113472.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(15951..16397)
                     /locus_tag="ON396_001380"
     CDS             complement(15951..16397)
                     /locus_tag="ON396_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3113473.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(16398..18320)
                     /locus_tag="ON396_001381"
     CDS             complement(16398..18320)
                     /locus_tag="ON396_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCR3113474.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT         5565 a         3706 c         2998 g         6182 t
ORIGIN      
        1 aaacctatct ttgcatatac taaatagacg tatatacaca gataggtttg tcttgtgaaa
       61 aataatgatt atccttacca aaaaatgttt gtttttctac atgttcgtac ttatcaaaaa
      121 atatgatcct tattgaaatt actggctatt tatattccaa cgacttcttg tgttaatttt
      181 tgttatctca tagtaaatta tgtttcattt tcacttaatt tagttgtcac gtgttcgtca
      241 attcgtaaaa acggtaaata tataaacaca cttgcggcta taattaatat ttgtaaaaca
      301 gctgctcgcc agtctccggc tgttgctaaa aatcccgata taactggtgg cgtagtccaa
      361 ggaatttgca ctactacatg attaattaat cccacagcag ttgcaaaata tgctactgtc
      421 aaagaaaaaa tcggtgcaag cacaaaggga ataatcagac tgatattaaa tacaataggc
      481 aatccaaaaa taataggttc gcttatattg aatatagcgg gagttaccga taatttagca
      541 aagtccttat aatcttttct acgactgaag ataaatattg caatcaataa agcaatcgta
      601 tttcccgatc cacccattac cgcaaaggta tctttaaagg ccatattgat aatatgtgga
      661 atttgctctt tgtttgcata agctgccata ttttcttgca tattctgaat caaaaacgga
      721 tctaacaatg aacctgatat tactgcttga tggataccaa atccaaaaaa tagattagca
      781 atagatgtaa ttaacaaaaa tcctggtaaa ccagttgtaa ttttacttaa tggctctgta
      841 actatcgtgg taattagctt atttatatcc atattaaaca attgaatgat tataaatgac
      901 aataaagcaa acgaggtaat cgtaataatg attgggatta atacattaaa agactttaaa
      961 acagcgggag gaatatttcc ggatagctga atctgtagtt tcttattatt agaaagttta
     1021 ataaataatt ctgtagccgc taatccaaca aatataccta caaacattcc tgaagctcct
     1081 gtataggata taggtatcac acccgtcact tcaacagctt tatccgcatt ttcaggagta
     1141 aacatcatct gcataggtgt taatacaaca aatgtcgaaa tagcaactaa taccgctgca
     1201 atatgatttg tataatttct gcttgcacac atatgataag atactgctgc tacaactaat
     1261 aatgtagtta tgcttaatgt accattggca attgaagtac caatttgttg taacgtggtc
     1321 aatgtactag tatcgacaat ttcccccatg aatcctgttg gttctaagat cactgaatta
     1381 ataagtgtca caaatcctgc taacatcata aatggcataa atgtagcaaa cgcatctcgc
     1441 aaagttctta aatggatctg attccccaac ttagtcgcaa ctataccaaa tttttcaaat
     1501 acaatgtcct tcctgctact tttgcccata ttttttctcc tttatgctta ttgtaatcga
     1561 ttactttttc cacccaaata atacatcact tatcaaaaaa agtaaatact tttaattaaa
     1621 tatatatatc ttttttagta taaaaaaata agctctgaaa aaataatttt cccgagctta
     1681 tttttcaaca aaaacagctt caaaaacaaa gtcttgatat aaatgtctcg ataccgaata
     1741 ctcgaaaggt ttaccagacg atagagcaac aactttgtct atctcaatca taaattcacc
     1801 tggatctatt tttaatattt ttgcgtcaaa ttcattcgca gtatctcctt tgatacgtat
     1861 gtgcgaagtc tttatttcca gacccagttc atttgtgata taagcatata cagatttctt
     1921 taaatgtttt ggttccaagc ctggtatgac tgctaatggc atgaatgttt gctcaatcga
     1981 gtatggttga caatcaataa aacggcttcg tacaatatta tatacatagt cttttacact
     2041 gatacttaat ttatcagcta cttttttcga tggaagttca attgagaaag tatggatttc
     2101 tgaatctacg tgttcttcac caaccacgct aaacaaacct tcgtttggac caagaactcg
     2161 tgagttatca attggtgttc tgacgacttc agtcccatac ccacttctac ttcgcaaaac
     2221 tccttcggaa acaagtaagt ctacgccttt ctttactgtt aacttgcttg tatgataaat
     2281 aactgccaat tcatctcctg taggaagttt ctcaccaatt ttatactctc catttacaat
     2341 ttttttcttt atatctaagt aaatttccat atacttcgca atttttttca atggtgaccc
     2401 tccaatcaaa aacatatctt catctatact ttaacataaa taaaccgata aaatccacat
     2461 caaaattata cacacaataa aaaagatata tatatttaaa caaaactatt tactttaagt
     2521 tgtaagcggt gttatcatta agtggaaaca ggaggtaaaa aaattatgac aactatgaaa
     2581 aagctaactt ttccagatga tttttggtgg ggttccgctt ggtcagctga gcaagcagaa
     2641 ggaaaaggag aaacaggaaa agcagagact atctgggatc gctggtttaa agaacaacct
     2701 taccggtttt atcaacgaat cgattcctct attacaaccg atcacatcca tcaatataaa
     2761 gaagatatag aactaatgaa aaaaactggg cataactcct ttcgcgtttc tatttcttgg
     2821 gcacgaatgt ttccaaatgg cggtaccgga aaagtaaatc ccaaagccat ttctttctat
     2881 cgaaaattat tttccgaaat gaataataac ggaatcaaag tatttgccaa cttatatcat
     2941 tttgacatgc ctgctactct tcaagataaa ggaggatggg aatcacgtga agtagttgat
     3001 gcttatgttc attacgctga aacttgtttt aaagaatttg gtgacttagt ttgtcattgg
     3061 tttaccttca atgaaccact tgggcctatt ttaggtactt atcttgaaga ttttcattat
     3121 ccaaacctca ttgactttaa acgcggagcg caagcagcat tcttcactat acttgcacac
     3181 gcaaaagcaa tacaagtatt taaagaacta gctcttccta gtaaaatagg cgttattcta
     3241 aatttgagtc ctacttttcc tcgtagtcaa aatcctgctg atgtgaaagc agcagaaatc
     3301 gctgatttat tttatacgcg gagcttttta gatcctatgg ttaaagggaa tttttctaaa
     3361 aagcttatcc tgttattaca agaatataat cagcttcctg aagattatac gaaagaagat
     3421 cttgtatgta tctacgaaaa taccgctcaa attcttggac taaactatta cgagccacgt
     3481 cgcgtcaaat ctcgttcttc tatggtggat acatctagtc catttttacc agagtggttt
     3541 ttcgaaccac atatcatgcc tggacgacga atgaacgaat accggggatg ggaaatttac
     3601 gaaaaaggaa tttatgattt gtgcatggat atcaagaacc attatggaaa catcgaagca
     3661 tttatctcgg agaacggaat gggcgttgcg gatgaagaac gtttcatgga tgatacaggg
     3721 caaataattg atgattatcg gattgagtat atcaaagatc atcttgctta cttatggaag
     3781 gcaataaatg aaggatgtaa cattaagggg tatcatcttt ggacatttat tgattgctgg
     3841 tcatggatca acgcctataa aaaccgatat gggctggttt cactcgacct tactactcaa
     3901 aaaagaacaa ttaaaaaaag tggcgaattt tacaagcaat taagtgatac aaatggattt
     3961 agatacgatg tagacttgtt agttgaataa tattctaaaa ttccacttga agtgaacaca
     4021 ttagaaaaca agaataatga gaaataccaa taacaaatac tttttcttca tacttcattt
     4081 atacttgtca gtataagaga ctgtgacaaa tgtagtgatc ctcagaagtt aggtctggaa
     4141 atctaacttg agaagatttt tttaaactac gtccagtctt ttttatttgt ttaaaattga
     4201 agttgacaag tcttcattaa aaatttagaa tattcacaag tttgacgaca attatgaaaa
     4261 tttatgtcgt gctaaaggag ttttgttatt ggatgaaaag ttagatataa aaactgcgat
     4321 caaagacacg ttacctaccg tttttggtta tatcggtatt ggacttgcat ttggtatcgt
     4381 tgggaaagct gccgggtttc atccgctggt cgtcacgttg atgtccctgc tgatctatgc
     4441 tggttctgcc caatttatca cggtcagcat gcttgctagc catagtccac tgctctccat
     4501 cgttctctcg acctttctag tcaattcccg aatgattctg atgagtatga cgattgctcc
     4561 ttatttcaag aaaaatcggc tgcttcaaaa tctgctgatt ggcacattgc tgacagatga
     4621 aagtttcgca ctgggaatga ataaattgaa ctatactgga caaaagttaa actttcgttg
     4681 gatgaataca gctaattgga tttcttattt gacgtgggtg ggttcttctc ttgttggcgc
     4741 acttttaggg aattttatta ctgatccgaa aaagtttggt ttggatttcg ccatcgtcgc
     4801 tatgtttatc gggttacttt atcttcaagt catttctgat agaaacacct ctaaacgatt
     4861 acagttgatc ctgattggtc tcactttgat tctggtatat gtcggactga tttttatccc
     4921 aagtaactta gtgattttag ttgtaacact gattgggtgc ggattggggg tgtggatcaa
     4981 acatgccttc ttttgagttt acattgctga ctattttagg ttgtacttta gcaacttggc
     5041 tttctaggat cctaccattt gtcttactga aaaaattcga tttgccgcaa ccgttgcttg
     5101 agtatttaag ttttgtcccg atcgtcatta tgtctgcttt atggttcagc agtctattta
     5161 cccaaaatat cggacatctg ccgcagatca atctcgaaaa tgctttggct tcagttccta
     5221 cacttttagc tgctattctt tcaaaaagct tattggtgat cgtcttggta ggaattcttt
     5281 ccctttcgtt gattcgctta ttataaataa aaaaacaaga gcttatggca aattactgtc
     5341 atgcgctctt gtttttatca aagtcaaata agaaaaaaat cggcgtattc ttgcttatcc
     5401 gttgaacgga ccactaacaa cgacttttcc taatatatgt cctgtttcta cgtctttggt
     5461 tgcttgtttc aagttcttcg cattcatccc atctgaatag accttgctca atgtactgcg
     5521 gataatccct tcatttgcta aagcagcaat gtgcgctaaa gcttctcctt gcgtttcgat
     5581 ttcatagtta tagtctgttt tagcgaacat atactcccag tcgaagctga tagattggtt
     5641 tttccaaaca cctatatcta acggctcttt gatccctacg atcgttccca catgtccaaa
     5701 tggtcggaca aggtgtttga tttgatcaaa gtagcgggta atatcgaata atacagcgac
     5761 atactctact tgatcgattc ctaattcacg aaaactttta cttaagtcgt gatgataatc
     5821 gattggatag tctacacctg tttttttcag ccattcaaaa ttcttcggac ttgcagtcgc
     5881 gtagacttct aggccagccc aatgcgctag ttgattcaaa atcgaaccga cgcccccagc
     5941 tccgttgatc actaagattt ttttcccgcg gttggcattt tcttctggag tcagaccaaa
     6001 tttttcaaat aacaattcat aagcggtcaa tgaggtcaac ggcaaggctg cggcttcttc
     6061 atctgaaaga ttttttggtg caagagcgac aatacgttcg tcaaccagtt ggtattcttg
     6121 attgcttcct gctcttgtcg ttgtacctgc gtaaaaaacg cgatcgccga cagaaaattt
     6181 ttgcacttca tcacctacag caacaacttt cccgactcca tcaaacccta agatacgtaa
     6241 agcatttctt atcccgttat cttggcgaag tttcgtatcg acaggattaa cagatactgc
     6301 gttgactttt accagaagat cccgtgaact gggtacaggt atctgttctt tttcatcaat
     6361 aaagcttttt ggatcatcga tcgacaaccc ttcataaaaa ccgactacct tcattaattc
     6421 cttttctgac atacgaattc ctccttagct tcatcaagtt ggagcatcca tacagttttc
     6481 atttcaatat atatagtatg ctatttgttc cacttttatt catcataatt attctcttac
     6541 ttaaagtaaa ctatttgcac ttttttggtt aaaaaagagg ttgcgacaag acttttgtca
     6601 caacctcttt taaacggtgt tcaatcaact gaccctcggt attcgctcaa aaaacgacaa
     6661 aatatgagga gctatttttg tttggctgtt cttattactt gatgcaggac agctttttca
     6721 cgacctctaa ttcacggtgt tcaagcaaga aatcattgtt cactgaaaag cgttggatgt
     6781 tctttttcaa aggtgtctaa gatctctcgt acttcatcag tagatttcgt attcatcaac
     6841 tgaactcgca agtcatttgc tcctggaaat cctttgatat agattttaaa gaaacgatgt
     6901 agtcctgtga tggaacgcgg gatgatttct gaataatgat cttgcagatc tagttgcaga
     6961 cgaagtagtc cgatcagttc tttttctgta tgttccctcg gttctttttc aaatgcataa
     7021 ggattcttga aaatcccccg gccaatcatg atgccatcta cgccatattt ttcggccaat
     7081 tcttctcctg tctggcgatc tgggatatct ccgttgatcg tgatcagtgt ttgcggagcg
     7141 atttggtcac gtaaagcaat gattttaggg atgacttccc aatgtgcatc tactttactc
     7201 atctctttac gcgtacgcaa atggacagaa agattggcta tatcttgatg aagtaggtga
     7261 ctgatccatt cttccatctc agacatttcc gtatagccga tacgcgtctt gacgctgaca
     7321 ggcaaaccgc cagctttagc cgcttcaatc aattgtgccg ctacttctgg gcgaaggatc
     7381 aagccactgc cttttccacg ttctgctaca ttagggactg ggcagcccat gttgatatcg
     7441 attccttgga agcccatttc tgccatagca atgctcattt ctctaaaaaa ttctggcttg
     7501 tctccccaaa tatgggcaac catcggctgt tcatcttctg taaaggtcaa tcttcctcgt
     7561 acactgtctt ttccatctgg atgacagaaa ctgtctgagt ttgtaaactc agtaaagaag
     7621 acatccggtg ctgctgcatg ctttacgaca tggcgaaata caacatctgt tacatcttcc
     7681 attggtgcta aaacaaaaaa aggctttggc agctctttcc aaaaattagt agtcatttat
     7741 ttgttactct ccattctatc aagcagtgaa gtcgcaatct ttctcaaaaa cattgtcatt
     7801 atactaggaa acaaaaaaag aagcaaagtg aacagcttaa aagatgtttc tgattgagca
     7861 aaaaaagact gtgacaaaaa tcttatcaca attccttttc cggaataaat ggtgttcaaa
     7921 agcttgcatc tgcggtaata agtccaacca aacgaaaaat aggaggagct atttttgttt
     7981 ggctgttctt attacttgat gcagaacagc tttttcacaa cctctccact aaacggtgtt
     8041 caatcagctg accctcggta ttagctcaac aaacgacaaa atatgaggaa ctattttccc
     8101 gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctctttt ctaacttctc
     8161 aatcctcttc ctttattgat caaataccaa gtaacggcgt acaaaacaac gatgaatagc
     8221 acaagtacga tcatcgatgt tgctactggc acatcgattg ttccaaggaa tccgtaacgg
     8281 aaaccgaaga tcatataaac gatcggattg actttcgaaa cagcttgcca aaatggcggc
     8341 aacatagaga tggcatagaa aacgccacct agataagtta acggctgcaa gacaaatgta
     8401 ggcacgatcg atacatcgtc ataagactga gcaaaaatcc cgttgagcag tcctgctaac
     8461 gaaaacaaga ttgccgtcat caataatgta atgatgacca tgaaccatga atacacatga
     8521 agtggtacaa agaacaagga aatgatcgtt acgagagagc cgacgagaat actgcgaccc
     8581 actccgccaa tcacaaagcc ccaaatgatc acatgcgtag gaacaggcgc taccaagatt
     8641 tcttcgatgt ttttctggaa tttttgagag aagaaggacg aagaaacgtt ggcatacgaa
     8701 ctagtgatag cagacatcat aatcaaacca ggcacgatga attccatata tgaaaaacca
     8761 cccatgtcac cgatccgacc accgatcatt tttccgaaaa taacaaagta caaggatgtc
     8821 gtgatcacag gcggaaccag tgtttggacc cagatacgca aatagcggtt cgtttccttg
     8881 atagctaaac tttttaatgc agtaaaataa agattaaaca tttttctcct ccgtatgatt
     8941 attttcttct gtgattttca aaaacagttc ttctaaacga ttcgacttat tcctcataga
     9001 aagaactttg attccttgtc ttgtcagctg atcgaagatt ccgttcaccc cttgatttcg
     9061 ttcaacttcg acagcgattg tctgttcatc ttcaaatgta caagcgtatc cttcaatcac
     9121 aggtttggtc tcatatggcg ctaaatcaaa gataaatgtt tcaaattgca gtttggatag
     9181 aagctctttc atactagtat tctcgatcag ttcacctgat tggatgatcc cgatgttgcg
     9241 acaaagcatt tcagcttctt ctaaataatg cgtcgtcaaa atgatcgttg taccattttc
     9301 gttcaattct cggagaaact cccacatctc gcgtctcaat tcaatatcga ctcctgctgt
     9361 tggttcatcc aagatcagca aacgtggttc atgcatcaat gccctagcaa tcatcagtct
     9421 tcgcttcatt cccccggaaa gcatacgtgc acggacattt ctcttttccc aaagattgga
     9481 ctgcttcaaa tatttttcgc tgcgtttcat cgcttctttg cgagagacac cgtagtagcc
     9541 tgcttgattc acgacgattt gctggaccgt ttcaaaggga ttgaaattga actcttgcgg
     9601 gactaatcca atctgttgct ttgctcggac tagatccgta tccagatcat aaccgaagac
     9661 attcactttt ccagatgttt tattgactaa agaagtaatg attccgatcg ttgtagactt
     9721 cccagctcca tttggtccta atagcgcata gaaatctcct tctttcacgg aaagatcgat
     9781 tcctcgcaat gcttcgaccc cacttccata aacctttttc aaattattta tttcaagcgc
     9841 atttgccatt tatttcctat ctccttatag acatgattct ttattgtatc atggaataca
     9901 gcagatgaaa aaacaacttg gttccagcga aaaatcaggt tgctgggctc aggaaaatct
     9961 cctaatgaaa gacatcaatc ggacgaagtc agacaacctt ttcagaaaaa acaaatcgaa
    10021 gataaaggat tactattcat atgtgcaaca aaaggagcgt gagacaaaag taaagaacac
    10081 ttttgtctca cgctctaaac acgtataaac ggcggaagca aaagccaaga tttttgatcc
    10141 aataaatact ttcgatcact tcgcctatcc caatacatgc cactgccgct tttcatcaaa
    10201 taacgcggct tccgtttttg cggtttctac taaagtctct gggtaatcca aaactttcag
    10261 caaggcaatc gcatttcggg tccttgaagg tccttctttt aatttgaaat caaaactgat
    10321 tccttttcct tctgttactt gttcttcaaa gtggacattc tcacaataat tctttaaaat
    10381 ctctgtcaat tcgatatcat gtgtggcgac aaatgctaaa gatggatact ccgcaagcca
    10441 ccgaacgact gaagcggaag aagcaatccg ttcgatcgta tttgtccctt ttaaaatctc
    10501 atctacgaag cagtagcagc gttctttgga agccacttgg tccaacaaac gtttgatcga
    10561 tttgatttct gaaacaaaat aactgtcgcc ttcaaataaa tcatcttcga ttgccatgga
    10621 agtcaacaca tgtccaggtt gcagggtaaa ctgttctgca atcgctgttt ggatcgtctg
    10681 tgctagaata cagctgatgg ctatactttt gacatacgta gattttcctg atgcattgga
    10741 acccgtgact aatgtattct tctgccagtt gaccggattc actaccgctt cttttaacaa
    10801 tggatgatag atatctttcg ctgtcacacc gccctcagta aactctggtc ggcaagtgat
    10861 gggcatataa gtacggtaat tcaatattgc agcggctact tctaattctc ccagcaattc
    10921 ccacagcttg atggcttcag ttgaatgctt ttctaattta gtgagaacga aatgatagga
    10981 gataaatggc agcatgaaca tgatgttgac atattcaaaa atcaactctg cttcactccc
    11041 actcttcata cgaaatgaaa aacctaattt agggatgtct cttaatggtt tagtttgctg
    11101 tttcagctct tcttgaagtg gtgtcggcag ttttgctagc tgattggcgg ctgaaatcgt
    11161 tgaaaccaaa taactcatgc tattcagctc tgtttctagt gtttgtttct tcactagata
    11221 atagaccgta ttgaatacta acccaccgat tgccaaatag attcctagta cttgtcctaa
    11281 aagaagcaaa cccaaaccca ccagcggaag caagccaaga aaaataaata aacctacgcg
    11341 tccaatcttt ttactttttc catccgctag atattgtttt gcaaaattat gatctttttt
    11401 tcctaaacga gcgaattgat attggatttt ttctctcagt tgaggatttt cctgataaaa
    11461 agcaatcagt ttttctaact gctgatcttc accaaaatca aagctgcgta aacgttgata
    11521 caacgcttct gaacctacac tagaataggt actgttgatt ccttcgaata cttcgaacat
    11581 atccaggtca taccaagtaa gatcatcgat ttcgctatcc catgaacgga acttcttctc
    11641 cgtgagccat gcttccttca agctttcttc tttatcaaat ctcggttgat aaggaatttt
    11701 ttcccatttg ctacgaactg tttcctttaa tttcaaacgt gaaaaaatgt ctaaagcaac
    11761 taataaaata acagctgcaa taataattaa aacgataaat tctctttgac ccatatccct
    11821 ctccgttcct ttatttgtta gctttagtat agcactgttg cgctatttga aaagagggta
    11881 aaaaaacacc tttcaaaata aaaggtgttt ttttcttgta tattacatat ttatctctca
    11941 tttatcaaaa ttgacaaata atcataggga ggaattgttg ttaattcttg tttattctcc
    12001 agtgcattga caactgtccc aagggaaagg atagcctcag gtactatttt ttcttgttct
    12061 gttggattag ctaagcgaag aacaaatcct tgttcatgaa aataagaagg ataaatcgat
    12121 gatacaatta agtcttcagg taattcaatc aatgaaaatt tacgaggaac atcaagttca
    12181 ttttgtaatg gccaaatctt gttatctaac ctgtgaataa atacattcag tttctgcttt
    12241 tgataagaaa tagattgttc taaccgtttt tctgcctgtt ttgccacttt gaactcatta
    12301 aatcttcctt cttctacatt aaatccaaaa gtgactttat actcacctat ctcttgagct
    12361 agcggtgttt ccatcatgac atgcccttca tttgtcgtat ctcctgaagc tcgcccaggt
    12421 cgccaagcaa gattaggttt tccaagttcc cctgttgttg aaaataaggt tatgattaat
    12481 tgattttcta tacgttcata ctcttttaaa ccatctgcat aaaacgtaaa ataatgatca
    12541 ttttctgcca cagagacact cttatcgaat atttctaaat taacaggttt ctctaccata
    12601 gtttctgacc aattcggagg aatgacatca ggggtatttt caataaatcc gttttgaatt
    12661 tgtgcaattg atttttcaga tttatctaaa acattaattg ctaagcgcat acgatgcgag
    12721 taaacagtat tatgaatttt gagtgtgcct tcaacgattt gactttctcc cagactgaac
    12781 gatatttcat aagatatttc ttccatttcg ggtttttcag ataaacgatc ttttaaatct
    12841 ttaggaagtt gagcttttcc gtaaacaact aatgtttctt gtaaagaatc tttatatact
    12901 ttagctgttt ctaattttag agatagttct gtatctcctt ctaatggtga ataatcatac
    12961 gtatcgccat catttgctga atcgatcaaa tgaacgaaat caagatattg tctatctcca
    13021 actatcaaat tcaattgccc atctttaaaa gaaagtttat atttattgtt acttatctca
    13081 gttttgtcta tctcgattgc tgtttctaat ttttcattat ttttctcaaa ttcaacaaca
    13141 gtgtatccta gtgctggcac atttacattt atttttatgt caagttcata ataagctggt
    13201 tcatcaaaat attcgaaacc tgtagcaatc atccgataag catttttccg ttctggaaaa
    13261 taaacttctt tttcaatgat attataggga ctacctttga agtgtatttt ctttgaatca
    13321 gctactacat gcacgatttt cgatccacta taattctcag tcgttgtgtt gaaaacaagg
    13381 acttcattcg attttagctt taatgcatca gccatccgtt ttacaataag gttttcgatt
    13441 ccgtcagcta tctcattagc ttctttgatt cgatggaaaa tatcttccgc aacattatct
    13501 gaaacacagc caccaatact atcatgagcc tggttttgca aaatcttttt ccataaccga
    13561 cgaagtaggc ctttactgat atggatacca tttctttctg caatcagcat gagcggctca
    13621 actcgtttta gaattttttg ttccaattta aaattttcta ttttcatttt tgttcttact
    13681 gagccaatag aacgatgaac acgagagtaa accggctgac gaagttcacc tttatattca
    13741 ggtaattctt ttttgtttct gataacttct acataagaag gataatctga aataatattg
    13801 tggaatttac tgtatttatt caacttctct acgacttgat caaaatttaa aatcattgac
    13861 ttttgatcta cacccgaagg tactaaaata tctttgtttt ttccttgcat atgaagcatt
    13921 tcaactgctt catccaactt ttcttttgca aattcaggaa tatctttaat tgcgtctaag
    13981 gtcatcattc cagtcatata accaaatgga aaattcatgg cataaacaaa tttatctccc
    14041 aatcctttcc agataaaata aggagatgga accatcgttt caaagtttaa tcctcgccaa
    14101 cacatgaaat tatcgatttc tacttgatgc aaaagtgtag gcaaattcgc attgaaccca
    14161 aacgtgtcag gcaaataccc taccatcatt ggatctccat atttaaggat cgtatcactg
    14221 attccaatca ttaagtttct taaaattgat tcagcatcca ctaataaggc atctgtttgt
    14281 gcataccacg gtcccacgaa tagttgtcct gttttcatca gtcgttggat aaccttaagc
    14341 ttttgcggat tgatctccaa atactcatcg acgattgaag tttgtccatc taaacaaaag
    14401 tttactttag ggttcctttc caattcatct agaacttcag taaagacctg atcagacaat
    14461 acgatggcat cttcatttgt aaaataccat tctctgtccc agtgcgtgtg ttgaattata
    14521 tgtgcattta ccatatcaga ttctcctact ttccttcttc tactgaattt acttgttgtt
    14581 cttttttata cttgatggat tttagtgcat tgaccattaa cgctgtaacc actcccccaa
    14641 taacaattga tactaaatat aaaggtatat tcgtaacaaa aggaattgcc ataaaggttg
    14701 aaagcattgt ttcttgagta acaccaaaaa atccatttag accacatgct actgcagtgc
    14761 ccaccacgat gcttggtatt actcgtaaag gatctttcac ggcaaacggg atcgtccctt
    14821 cagtaatccc cacaaggccc ataatcatag ctgtctttgc attatctcgt tccgattcat
    14881 cataaagttt tttgtttaaa attgttgcta aaaacattcc aaatggcgga atagcaattc
    14941 ctaataacat ggctgtacta ggtccaaagc ttccgcttga aaaagaagca accccaaaag
    15001 catatgcgat tttattgata ggtcctccca tatcaaacgc catcattcct cctaaaatag
    15061 ccgacatcaa agcagcattt gctcctgaca tgccttctag ccaattagtc agtcctgttg
    15121 tcattgcttg aataggcgta gctagtacat accataatat gattccaaca actaatgtag
    15181 taacaaaagg aatcagcata attgatttta gactaataac ttgcatcggt acttttactt
    15241 ttttcaatgc attagctaaa tagccacaag ctaatccagc aaccattcct ccaataaatc
    15301 ccataccaag attttgacac atcatcccag tgataaaagc tggtgctaga ccgggacggt
    15361 cagcaattga atatgcgata taggctgaaa tcatcggtac gataaactgt aatcccgtct
    15421 gaccaatcat tcgcaggact ccccagaatg tatccgtctg gtcccaaaca cctgtgccac
    15481 caaaaatgac tgcaatagaa gttagtaacc ctgctgctac gacaagcggg atcatatagg
    15541 aaaccccact ttggaaatga tttagaattt ctcttccttg tttcttgaat tccattagta
    15601 actcccccac gatatatttt ttatttgtta attaatgcaa ttgcttcatc aatcgtctct
    15661 tcaccatctt ttactacctc tgaggtccct actgttaaaa tcggtttacc gtcaaagcga
    15721 cctcgttctg aaacatttgt atctactgca aatatgacaa catctgcctc ggaaacatcc
    15781 gaaattgaaa gtttgttttc tatccccata gccccctgtg tttctacttt aatatctaac
    15841 cctttcttct tcgcaaactt ttctaaatta gcttttgcca tataagtatg ggcaatccct
    15901 gcgatacatg ctgttactgc tactattttt gtcatatgaa attcctcctt ttaaaatgat
    15961 agagctttta ccaattcttt tttatttctc gaatctttta ttcttttaac aaactcgtca
    16021 tccatcaatc ttccagataa ttctgctaaa atacgtaaat gattagtacc tttatcagtg
    16081 tcttttattg ctaacaaaaa gacgagatca actggttgat catctaatgc gccccattca
    16141 acagaccgct ttaacttggc tacagccact gttgattcaa ttactgctag gcttttgcca
    16201 tgaggaatgg cgatattttt cccaattcct gtggtcatat ggttttctct gtcaaatacg
    16261 tctttttgaa attgtttgtg gtcagacaca aacccattag tctctaacaa tttcgataat
    16321 ttttcgacta cacttttttt atcagatgca tttaaatcta tcactatatt atttatattg
    16381 attttttcac tgatcattta taagccttct ttcaatatat aaactaattt ttcaaacgac
    16441 gaactcgttt ttaaatttgc taataactca tgattcgaag cgatttcatt gaatgtttca
    16501 taaattttat tcaattcagg ttttactttt tcattaagtg caaaaaagaa aacagaatga
    16561 acaagtgtcc catcccaatc aattccttct ggattaacgt agacacagat tactggcttt
    16621 tttataaaat ctacttcgat atgcggcata gcaaaatcat tcatcgctgt tgaagatagt
    16681 ttttctcttt tgattgctcc ctctagtatc ccttctttcg cgtaattttc ttttaccaat
    16741 tttttggtaa ttatatttaa aacgttcact tgattatcct ttatttggtg ggtaaaaaag
    16801 caaaatcgtt tatctaataa atttagaaaa gcatcttctg tttgttcatt taactgtaga
    16861 tactgtttta cttttctgac atctgcccct aacatcaaag gactaacaac aataacagga
    16921 aaatttgtat tttcaatcgg aatcgtgctt ataacaagtt catcagtgac ttttctatct
    16981 tgtagttcat tgatgcttag tacatcttct atctcaacca tatctgcaaa accgttattt
    17041 attctttgct ctaataattt tgatgttcca tatccgctac tacaaactaa tatcactttc
    17101 gttttgtcag gtttgtaacg atccaaaagt gattgaatat ggagtgttac gtaagcgatt
    17161 tcatcttcat taaaatgtat acagaatctc tcttcgattt cctctaataa atctacactt
    17221 atcataaatg accttttaaa agaatatttg attttgtccg tataagggtt atagatattt
    17281 actcccaatt tgagtctttt aactgctgca ttgagatgta atactagatt attgatcaat
    17341 tcttcatcag gataaaattc atttaattta attcttacta actcttgtag ctcaacaccc
    17401 atatctaaat aggaaagctt cgtttgatct aagtcgtgat tcatagcatt tttttcaatc
    17461 gaagtaatgt gaatgtcaac ataagcttgt tcatctattg gcacaataat tgaaaatgct
    17521 ttttcaatca ttttgattaa aataagtgta ttctttgaga tttcttcttc agaattatta
    17581 ctacgtgata agaaattgct tgtttcaatc ctttctatcg caatagcaag gtggatagct
    17641 aaagtttgaa attcataatc tgtaaatttc aattgtgtct tattcatgta tcgattaagc
    17701 aattcggcta cttttttcaa tatgtcctta tttaagaaat tattcgcttc accaatgaaa
    17761 ttgatattca agtctaagag atctgtttta ctgttagatg ctgtaaagcc accccaataa
    17821 tagttcacaa cttctgacat caaccttctt ttactttttt catcagcttt gatgcgcacg
    17881 ccatttcgat ctgaaactaa ggacatccca tgcttgttta gtctttttct aacatctttc
    17941 attgtgcttt ctaccgtcga cctgctcacg tataattcgt cagcaagatt ttgaatcttt
    18001 agagaatcat tagtattcaa taatttagca aacacataag ttagacggtc ctccggagag
    18061 tctgcgatat tgattttatg actggataat aaatttcgaa gtgtttcttt ttttccaata
    18121 agcgaaacgc cttttttagg ttgaaccaac atttgtacat ctggcatttt ttggattatt
    18181 tctttcaaaa ttaaaatatc tttgctaatt gttttcgtag atacatataa ttgttctgat
    18241 aattctttgt aagttgtgtg ctcattttca atcaaaaagt atagtatttt catttgccgt
    18301 tcgctaaatt cgatgcccat tatcttcacc tcctgaatac agcttaagtg tacaattatt
    18361 atctctattt tcatacagaa gaaagttctt gattaatgta gaagtttcta atattcttga
    18421 ttatttcaaa tcaataaaaa agcctgcaat a
//
LOCUS       DAJSKS010000048        18429 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJSKS010000048 DAJSKS010000000
VERSION     DAJSKS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.049"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            172..516
                     /locus_tag="ON396_001382"
     CDS             172..516
                     /locus_tag="ON396_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR3113475.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="ON396_001383"
                     /pseudo
     CDS             517..936
                     /locus_tag="ON396_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1028..1378)
                     /locus_tag="ON396_001384"
     CDS             complement(1028..1378)
                     /locus_tag="ON396_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113476.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="ON396_001385"
     CDS             complement(1344..2675)
                     /locus_tag="ON396_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HCR3113477.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="ON396_001386"
     CDS             2931..3497
                     /locus_tag="ON396_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3113478.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="ON396_001387"
     tRNA            3642..3729
                     /locus_tag="ON396_001387"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            4063..5037
                     /gene="bsh"
                     /locus_tag="ON396_001388"
     CDS             4063..5037
                     /gene="bsh"
                     /locus_tag="ON396_001388"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCR3113479.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5362..7257
                     /gene="asnB"
                     /locus_tag="ON396_001389"
     CDS             5362..7257
                     /gene="asnB"
                     /locus_tag="ON396_001389"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HCR3113480.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7452..7898)
                     /locus_tag="ON396_001390"
     CDS             complement(7452..7898)
                     /locus_tag="ON396_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCR3113481.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8126..8275
                     /gene="rpmG"
                     /locus_tag="ON396_001391"
     CDS             8126..8275
                     /gene="rpmG"
                     /locus_tag="ON396_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR3113482.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8363..8881
                     /locus_tag="ON396_001392"
     CDS             8363..8881
                     /locus_tag="ON396_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3113483.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8958..9914)
                     /locus_tag="ON396_001393"
     CDS             complement(8958..9914)
                     /locus_tag="ON396_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3113484.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10080..10886
                     /locus_tag="ON396_001394"
     CDS             10080..10886
                     /locus_tag="ON396_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3113485.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10883..11872
                     /locus_tag="ON396_001395"
     CDS             10883..11872
                     /locus_tag="ON396_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR3113486.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11869..12873
                     /locus_tag="ON396_001396"
     CDS             11869..12873
                     /locus_tag="ON396_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR3113487.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13005..13547
                     /locus_tag="ON396_001397"
     CDS             13005..13547
                     /locus_tag="ON396_001397"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR3113488.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13567..14265
                     /locus_tag="ON396_001398"
     CDS             13567..14265
                     /locus_tag="ON396_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR3113489.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14286..14498
                     /locus_tag="ON396_001399"
     CDS             14286..14498
                     /locus_tag="ON396_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCR3113490.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14498..15460
                     /locus_tag="ON396_001400"
     CDS             14498..15460
                     /locus_tag="ON396_001400"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCR3113491.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15478..15873
                     /locus_tag="ON396_001401"
     CDS             15478..15873
                     /locus_tag="ON396_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR3113492.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16036..16401
                     /locus_tag="ON396_001402"
     CDS             16036..16401
                     /locus_tag="ON396_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HCR3113493.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16398..18209
                     /locus_tag="ON396_001403"
     CDS             16398..18209
                     /locus_tag="ON396_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCR3113494.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18271..18417)
                     /locus_tag="ON396_001404"
     CDS             complement(18271..18417)
                     /locus_tag="ON396_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HCR3113495.1"
                     /translation="MLVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRK
                     RVSR"
BASE COUNT         5924 a         3119 c         3810 g         5576 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggtaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaacttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat aatagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatcaaaga ttagaggttg tgaagaagct gtcctgcatc aagtaataag
     3781 aacggccaaa caaaaatagc ttttcctatt ttttcgtttg attgggctta ttaccgcaga
     3841 tgcaagcttt tgaacaccgt ttgttcggaa taaggggctg tgacaagact tttgtcacag
     3901 ccccttattg ttgtactttt aacctacaat attttttgat tccccctgag tactattttt
     3961 tataaatgct agtaaatcaa tcttctttgg cctttttttg ataaaaaaaa tactagatgt
     4021 aagataaaag tgtaaaaacg atttgtttta ggaggggaaa cgatgtgtac cgcaatcact
     4081 tatgcaacga aagaccatta ttttggaagg aattttgatt atgagatgtc ttataatgaa
     4141 gtggtcacta ttactccaag aaattatcgt ttcgatttta gaaaggtaaa aaatttagat
     4201 aaacattatg caatgatagg aatagcagct ggggtatcca actatccact ctattatgag
     4261 gctacaaatg aaaaagggtt gagtatggca ggactgaatt ttcctggcaa tgctgattat
     4321 aaagaattac aagaaggaaa agataacgtt gcaccatttg agtttattcc atggatacta
     4381 gggcaatgct caaccataga cgaagctaaa gaattattgg ctactatcaa tctagtcaat
     4441 atcgatttta gtgaaaaact acccttgtca cctttacact ggttattagc tgataaagaa
     4501 aaatcgatag taattgaaag tatgaaagat ggacttcatt tatatgataa tcctgtcggt
     4561 gtgttgacaa ataatccgcc atttgattat caattattta atctgaacaa ttatcgctcg
     4621 ctatcaaatg gaacaccaga aaatcatttt tcaaaccaga tcagcttgaa tgtttatagt
     4681 cgtgggatgg gaggattggg cttacctgga gatctctctt ctgtttctcg atttgtaaaa
     4741 gccacattta ctaaaatgaa tgctgcatca ggtgattcag aatctgaaag tatcagtcaa
     4801 ttttttcata tactaggctc agtcgagcaa caaaaaggag tatgcgatac tggagaaaga
     4861 aaatacgaat atacgattta ttcttcttgc tgtaacgttg ataaagggat ttattattat
     4921 cgtacgtatg aagacagtca aattactgct gtaaatatga ataaagaaga cttggatcgt
     4981 catgaattgg tcagttatcc agtcataaaa gagcaacaga taaactatgt caattaatta
     5041 atgaatagat ccgtttggta tcatattttc tgatacagtc ataattagac acgatcttta
     5101 ggtcgtgtct ttttttgctg gtttttttgg aaatagcatc cactaatatt tttttaacac
     5161 gaagagaata aggtagcctc aaaaaagaaa acatggtaaa ttagatattg ggactttatt
     5221 ttctgtaaaa acagaaactt tcatcttttg ttttcacgtg acgaaattgt aagagagaaa
     5281 atattcgtcc gctatttatt ctatgataag atgcatatga atgaaataaa cagtaatttt
     5341 atattaggat gtgaaagtta aatgtgcggt attgtaggtt ttgttaacga caaggacaac
     5401 aaaaaaacaa ttattaatac aatgatggat cgaatcgttc accgtggacc taatagttca
     5461 ggagaatata tcgataaaca tgttgcttta ggattcagaa gattaagtat tattgactta
     5521 gagggcggta ctcagcctat ttacaatgaa gatcggacaa agatcattat ttttaatggc
     5581 gaaatctata attaccagcc tttgagagaa gaattgattg cagctggcca cgtttttcaa
     5641 acacatgcag atacagaagt ccttttgcat ggttacgaag aatggggaac agaattattg
     5701 caaaagatcc gcggaatgtt tgcttttgct atttgggata atgaaaagaa tgagttattc
     5761 ggtgcaagag accactttgg gatcaaacca tattattatg cagaaatgaa cggaacattt
     5821 atgtttggtt cagaaatcaa aagttttctg cctcatcctg attttaacaa agaattgaac
     5881 aaagaagcgt tgaaacctta tatgactttc caatattctc cattaaacgg cgaaacattc
     5941 ttcaaagatg tctatcgttt gcctgaaggc cattattata cgtataaaga tggaaaaata
     6001 gacattcagc aatattggga tgcagatttt gaaacaaaag aaacgcattc ccgacaagaa
     6061 tggatcgaaa aaatcgacga aaccgttcaa gcttctattg aagcacatac ggtcagtgat
     6121 gtagaagttg gttctttctt atccagcggg gttgattcca gttatgtaac ctctgtattg
     6181 aaaccagatc attctttctc aatcgggttt gatgataaaa cctataatga agcgattgaa
     6241 gccagaaaac taaccgaact attggactta gataatacag cagcagtgat cgacggagac
     6301 atgtcgttca aagcattccc tttgatccag tatcacttag atgaacctga ttcaaatcct
     6361 tcttgcgtac ctttgtactt tttagcaaat cttgcttcac aaagtgtgcg ggtagtccaa
     6421 tctggagaag gggcggatga actgtttgcg ggataccaaa cgtatggatt ccatacgaat
     6481 tcaaaattca tccgtgtgat tgctcaagga ttgaaaaaat taccaaaagg aacacgctat
     6541 aatttaggac gtaaaatcgg aaaaatgaaa aatttccatg gacggatcca tttatatgaa
     6601 tcacttgctc ctgcaaaaga atattttatc ggtcatgctc gagtgttcga agaatcagaa
     6661 gcatcagaag tgttaacacc aaaatatcaa acagcaccat ctgtggatga aatcatgact
     6721 gttcattacg agaaaacaga aggaatcaaa gatgaagtca ataagatgca atatgtcgat
     6781 cttcatcaat ggatgccaaa agatatcttg ttaaaggcag ataaactttc tatggcaagt
     6841 tctttggaag tccgagtacc tttattggat atcgaagtca tgaaattggc tcaacaaata
     6901 ccaagtaagt ttttattgaa tcaaaacaac acaaaagata ttttcagaca agctgcgaac
     6961 aaacatcttc cggaaaaatg gtcgaatcgt gtgaaattag gattccctgt gccaatcaaa
     7021 gcttggttga aagaagaaca cggctacgaa caagtcaaag cattgttcga agcagatttt
     7081 gcaaaagaat tttttgatca agaaaaaatc atgaagttgc tagatgacca tcacgaagga
     7141 cgtaaagaag agcaaagaaa aatctggaca atcttcagtt tcttgacatg gtataaagtc
     7201 tactttgtag atgaaacgat tccgcaagca gaagcaatcg actatgtgac tgtttgatga
     7261 aaatcaaata tcaataaaaa agaggttttc tcctaccgtt taacactgag tgataagaaa
     7321 caatttcaaa aaacagctct tcccgtttta tagatattat gacttatggt tcggagttgg
     7381 cagaggaaca ccattgtttc gaacataagg gggttgtgac acgattttcg tcacaacccc
     7441 tttacatatg ttcaataatc gatttctttt tcttttgtcc catctttttt ataaatgaac
     7501 aaagttgatt ctttgttttg tgctacttgc tttccttttt cgattgcttc tttttttgta
     7561 tcaaaatggt tgcttgcctt ttttgcccct ttagatttca cgatccattg ttcttcctca
     7621 tattctacga tcacatctga atcaagcagt tttccagcac gaggattcgt atcgtgttca
     7681 tccgtcttgc ttggattttt ttctttttcg aaagttcgtt tttctgattc actagcatct
     7741 tcataccatt tttttgcttg atcaatggca attgggatcg cacggttatc agggtaccct
     7801 tcatctaata atgcatttgc gatgtcgatt gctttttttc ttgtcaattt atctaagttt
     7861 ttcattgatg caggaaagtc cttcatgttc catggcatat tagtatcccc ctttattaaa
     7921 aaacataatc tatataaaca agtgtaaggg tatttataca aaaagaaaaa gattttgttt
     7981 gagaagtaaa accgttgtat ttatcgtcaa gtagttttac ttaacaaaat cgtgaaaaac
     8041 actagcaatc tagaaaaaat catggtaaga taatcaagtc tgagagtcat cttagaggat
     8101 tcttgtaaaa atgggaggga aaaacatgcg cgtaaacatc actttagagt gtacttcttg
     8161 taaagaacgt aactatctaa caagcaaaaa caaacgtaac aatcctgatc gtttggaaaa
     8221 acaaaaatat tgcccacgcg aacgcaaagt tactttgcac cgtgaaacaa aataatgttt
     8281 ttaaagttct caattccttg ctggtcaaag aattgagact ttttttatct caaaacatct
     8341 aaaatcataa ggagcggata atatgtttat ttccatgtgg gcacaagata aaaatggtct
     8401 gatcggcaaa aatggtttgt taccatggcg attgccaaat gatatgcgtt tctttcgcga
     8461 acatacaatg gatcgaattt tagtgatggg aagaaagacc tatgaaggaa tgggggatct
     8521 ttcacttcct tatcgccata tcatcgtgtt gacaacgcag ccaaattttc gcacgaaaga
     8581 aaatgcggaa gtaatgcatt cgatcgatga acttttgtcg tatagtcaaa cgatctcaga
     8641 agatatctat gtttcgggtg gtagtcggat ttttcaagaa cttcttcctt acactggtcg
     8701 gatctggcga acattgatcg atagcacgtt tgaaggcgat acgtatattg ggaatattga
     8761 tttttcagac ttcgccctta tagaggagta tgaggggata acggatcaag aaaatctata
     8821 tgcccatcgt tttcaaaaat gggaaagagt aaaaaatagg gttgcttcaa aagaagaata
     8881 aaatctatgc gtctagtttg ttaataagaa aaaaagcata tcacgactaa tcgtgaagat
     8941 gcttttttta gttaaaatca atttagtttt tctttgatat cattgaccat ttcctgactg
     9001 gcaataggtc cgttatatag ccaactagat tcaggtccga attgcagtac atgattgtct
     9061 gtcactgctt taagattctt ccagcttggt tcattgaaca tggcagcatc tttgtcactg
     9121 ttcacaagga tgatgtagtc agcatctaat tcagctagct tttcagagga aacagcagac
     9181 cagtcagaag tggctttttt ggagacctct tccactagat caggaacacc aaattccaga
     9241 tcgtcgtata cgattcttcc acttgaccga tttttgctga ccataaaggc tgagttgttg
     9301 gtcacccaca acactgcagc tgtcttgttc tcgattttat ctgctagttc ttctcgagtt
     9361 ttcttgatgg atttttgata atcggaaatg atcttttctg cctctttttc ttttgccaaa
     9421 gctttgccga catcttttag ttgttcttcc catgtgacat ctgttccgtt ttttacaaca
     9481 taagttggcg cgattttgct atattgttcg tatttcccgc cttcgacagt agcggaagaa
     9541 gaaatcaaca gcaaatcagg ctcaaagctt aacactttct cataaggaag gtcataagaa
     9601 atcgttggga catcctttaa atcatcttgc agataatctt gaatggaacc actgccaaca
     9661 gtccactggg cgataggttt ttcccctagt gcaatcagat agtcttctaa gtaactgccg
     9721 atgatccgtt tgggcgcgtt tggaatctct actttatgtc caagtgtatc tgtgactgta
     9781 tgagtactat tggatgaagt cgtcgaattg gcagttgtag ctgtttcttt atctgaagag
     9841 ggggagcaag cgctcagtac tccagccagc agcaatatgc tagataatgt gaatactttt
     9901 gtttttaatt tcatttcttt cctcctaaca taattgagaa tctttctcat tggttattat
     9961 tcctgatatt tcgaacgctg tcaaccgctt catttggcta tagatatgaa agtagcgctt
    10021 gacgaaatgt tgaaacagcg tcataattga gaatgatttt cagcgaaagg atgggtgaaa
    10081 tgacgagatt aagagcagct gacattgcag ccggctacga aaacaaaaaa atcattgaaa
    10141 acatgtctgt tacgattcct gaagggaaaa tcactagttt gattgggcca aatggaagtg
    10201 gaaaatctac tttattgaaa agttttaccc gtatactacc tgtgaaatca ggaaagatca
    10261 ttgtcaataa ctctgatatc tctacataca gttcaaaaga acttgcaaaa aaaattgcat
    10321 tattagcaca aactagtgaa caccctacag gtatgacagt agaagaagta gtatcttacg
    10381 gacgataccc ttatcaaaaa ctgttcagtg gcatggacac gcaagatcat gaagcaatcg
    10441 agtgggcgtt ggatgctaca ggtctagctg gatttcgtat gcgggatatc gcaacgctgt
    10501 ctggtggaca aacccagcgt gtctggatcg ctatggcgct tgctcaggaa acagatatct
    10561 tgatcttgga tgaaccgaca acatttttag atccggcaca ccaattagag atcctacagt
    10621 tattgactga aatcaatcaa atgaaacaaa caacgatctt gatgtcgatt catgacatta
    10681 atcatgcttc acgtttttca gattatttat tagcaatgaa aaacggtaag ttgatcacag
    10741 aaggtacacc tgaagaagtg atcacaaaag agtgtatgaa aaaaatattt gagattgatg
    10801 caacgattgt cacgcttcca agaacaaata aaccagtttt tctaacctat gatttgttgc
    10861 caaaagagca ggaggatcaa ttatgaggac tatgaagaaa aatagactcc tgcttttggt
    10921 ctgttttgtt cttttgatta ttttttttgt tgctctttca aatggcgcag cgactgtggg
    10981 aaaagcagat ttttggcagg cgttgtttga ttttgataga tcaaaacaaa gtcaccaaat
    11041 catacgaaat attcgtttgc ctcgtgtggc gggagcattt cttgtaggga gttgttttgc
    11101 attgagcggt gcattgatgc aaggagtgac aagaaatcct ttagcagatt caggcttact
    11161 aggaatcaac gcgggtgctt cgttggcaat ggcccttagt tttgcttttt ggcctaaagc
    11221 ttctgatttt tctgtgtttc taatgtcatt ggcaggttct tttgctgtaa ctttgctcgt
    11281 atttggtttt ttgcagtttt cgaaaatggg catgaatcct ttacagctga ttttagctgg
    11341 cgtagctatt agttcttttt ttactgctat cagccaagca cttacccaat tctttaatct
    11401 gcagcaggat cttgcttttt ggtttgtagg cggagctgcc aatatcacat ggaaacaatt
    11461 agcagtattg tttcctttat atatacttgc tgttttggtt tctttttcat taggaaaagc
    11521 aatatctttg attcatttga gtgaaacaca tgcattagct cttggaagtc atcctaaacg
    11581 aacgcggttg attgttttct gtctagttgc agtgttagca gccattgccg tttcgttagt
    11641 cggacctgtt tcatttattg ggctgttggt tccgccagtt gttcgcggaa tggttggaac
    11701 ggactatcgt tatgttctac ctgtctcttt tttagttggc ggtggattgg ttatgttggc
    11761 ggattttaca gcgaggatga tcaatccccc ttttgaaaca ccttttggat tagtcattgc
    11821 attgattggc gttccgtttt tactgataca agtaaggagg gaacaacggt gaaaaaaata
    11881 tattggacgt tctatttctt gctttttgta cttgtcatcc tgtctatagg tagtttaatg
    11941 attggtacgc cttttattta cctggatcag ctttttgcca cgttcatagg gaaagaatca
    12001 tcttctttgg cgctgattgt tagagaattt cgagttcctc gattactgat cagtttatta
    12061 gcaggagctt gtcttggcgt ttctggttat atcttgcaag gagtaacaag aaatgaattg
    12121 gcagattcta gtatattggg cattaatgcc ggagcaggat ttttagtgat gctttattta
    12181 ggtttctttt cgcactattc gctgccgttt ctattgctag ttgtagcctt tctaggtggt
    12241 gttttagctg cttgctgtgt ctatcttgcg gcctatgatc gaaatggctt tttgggtatg
    12301 aatcgaatct tattgtccgg tattgccgtt aatgccggcc tttctgcatt aacattgcta
    12361 tgcacgatcc aattatctaa ggaaaattat ggatttgtga atgcatggtt agcgggatca
    12421 atctggggtg cgagctggcc ttatgtttgg gcattgcttc catgggcggt tatcttaatt
    12481 ccgctaggag gactttatgc acagaggatt gtattgctat catttggtca agaacgcgcg
    12541 caaggattag gccttaacgt ggctaaaagt caaaaaatcc ttttgttgtt agctgttgct
    12601 ttggcaagcg gtagtgtagc ggtgacaggt agtttatctt ttgtaggact actagctcct
    12661 catgctgcga agctagtcgt acgtaaagaa aataactgga cctttgttct gagcggtttg
    12721 ttcggttcca tttttctgct tagtgcagat atggttgcta gagtgattct actaagtggg
    12781 gaagtaccta caggaatttt gattgccctt tttggtgcgc catattttct gtatgcactt
    12841 tttatcagac aaaaacacgc gctgaacagt tgaaattcat taaaaaagga aggccaatca
    12901 tttctaggtc ttctttttat tctatttatg catttatttg aaagttcgat tgctattttt
    12961 ctttttttcc tataccattt tatgagagaa ggaagggtga gtttttggac caattgaaag
    13021 taagattacg ccggaaagga atcgaaaagc tgaaaaatct ggccaaaagt ggcaagaaaa
    13081 aggaaaaaga acagcgaatc tataaacagc tattcgccag cgagtactgg aagtcagcaa
    13141 aagtgatagg gacgacttta tcgaatgaat ttgaactgga tacacagccg gttatccaac
    13201 gagcagaaaa agaacaaaag aaaatagttg tacctaaaac tttgccgaaa cgtcagatgg
    13261 cgttttatga aatggatgaa cagtccgttc ttgtacgttc gagttttggt gttcttgagc
    13321 cacaatcaga gaagctctat ttgccggaga tgatcgatct cctcattgta ccaggtatta
    13381 tctacaatag agccggttat cgaatcggtt ttggcggtgg ttattacgac cggtatttaa
    13441 ataattttca taaaaaaaca tgtagccttg tttttcgtga acaattgaat gaacaatggc
    13501 aaccggagag ctttgatcag aagatccaac aattgtttat tgactaaaat gaaatgggag
    13561 gttttcatga attatcaaca gcaaataaaa ttgcgtgtat ggttaagacg accgttatgg
    13621 acctatgctt ttttagggat acagacagcg gtcttcctca tgatggaatt atttccgcga
    13681 cttgagatcc cttattatgc cggaatgtat ggtccttatc ttgttcactt caatgaatgg
    13741 tggcgtttag tgacaccaat cttcatccat tttggactga tgcatttcgt aatgaactca
    13801 ctgatactgt attttatggg gcagcaaatc gaggcaatct atggccattg gcgttttttc
    13861 ttgatttata tgttcagcgg gataatggga aatacagcaa gctttgcttt taacgaagcg
    13921 aatgttctgt ctggtggtgc aagtacttcg atattcggtt tgttcggtgc gttgtttatc
    13981 ttagggtttc attttaaaca taacccagcg atccagcagc tagtcagaca ttttctttta
    14041 tttattgtgt tgacatttgt ttttggtctt ctggatacct ctgtagatgt ttggggacat
    14101 gtcggtgggc taattggcgg attagtgtta gggaatgttt tgggtctacc gaaacaacag
    14161 acaagctatt cgatacacca aaggattctt tctacactag tttttgtatt tctttttgta
    14221 atatgtattt tactgggttt aaaaaaatat ggtttacttg tataatatga aggaagtgct
    14281 taaagatgga gagcttatat gatgtacagc agctgttcaa aaaatttggc atctttatct
    14341 atgtgggtgc acgcatctat gacattgaat taatgactat cgaattgcgt aatctgtatg
    14401 atagccgttt gattgatcat gatacataca tgacagcgtg gcgcatatta aaaagagagc
    14461 accgaattga agaaagccga aagaaaggaa atttgtaatg gacaagaaga ttattgggat
    14521 tgatttaggt ggaacaacag caaaatttgc aattttaaca ccagaaggag agattcagca
    14581 aaaatggagc atcgatacca atattttgga tgatggtaag catatcgttc ctgaaatcat
    14641 tgaatcaatc aatcatcgtt tgaatttata taatatgaaa gccgaagact ttatcggaat
    14701 cggcatgggt accccaggaa gcgtggacag cgaagctgga acggttattg gtgcgtataa
    14761 cttaaactgg acagaagttc aatttgtgaa aaaactgatc gaggcaggaa caggaatcaa
    14821 attcgctatc gataatgacg cgaatgttgc tgctttaggt gaacgatgga aaggtgctgg
    14881 agaaaacgat ccagacgttg tattcgttac tctaggaaca ggtgttggcg gaggtatcgt
    14941 tgctggcgga aatttgattc acggcgtgag cggagctgga ggagaaatcg gacatatcac
    15001 tgtggatcct gaagggttcg aatgtacttg cggtaaacgc ggatgtttag aaactgtatc
    15061 tagcgctaca ggtgtggtac gcctaggtcg tttcttggca gaagaatacg caggggattc
    15121 aaaactgaaa gcaatgctag acaacggaga agaagtaaca agtaaagata tctttgaaat
    15181 ggctcaagaa gacgatccgt ttgcattgat ggttgttgat cgcgtatgct tctacttagg
    15241 tttagcttgc ggaaacttgg ggaatacatt aaatccgtca agtatcgtgt taggtggagg
    15301 agtatcggca gcaggagaat ttttacgtag ccgtgtagag aaatatttta aagaatttac
    15361 tttcccgcaa gtaagagaat caacaaaaat caaattagca gagcttggta atgaagctgg
    15421 tgtcattggt gctgcctcat tagccttgaa atttgcttaa aaaaacggag gcattacatg
    15481 gttttatggg taattaatat cattttgcta ttgattgtgc ttgcaatcat cctttgggaa
    15541 gtctatctac gagtaatggc caaacgttca gcaacgactt tgacagaaga agaatttcga
    15601 gaaggaatga gaaaagcaca agtaatcgat gttcgcgaaa aagatgtgtt tgatgctggg
    15661 catattttag gtgcaagaaa catcccttac actgttttga aagattcatt aggatctatt
    15721 cgaaaagacc agcctgtcta tatttacgat caaaagaaaa gtttgagcat ccgtacagca
    15781 aataaattac gtaaagcagg ttatcaagag atttatttac ttaagggtgg atatgacgga
    15841 tggtctggaa aaatcaaaag taaaaaagta taaaacagat gattgtatga cagctttaaa
    15901 aaccgaatca atcattagat tttgaccaag atcacatgaa cggcgcaatg gatagttgtg
    15961 ccgttcattt tttttatttt gtgctattat ctacatatat aagctaagcg aaaaggtaga
    16021 aagaaggaaa caactatgat caaattaaaa cgtgtttacc ttgatgctga aaaagaggat
    16081 ggctatagaa tactcgtgga tagattctgg ccacgaggaa tttcaaaaga aaaagcgcag
    16141 ttagatctgt ggttgaaaga aattgcacct agtaatgaag cacgcaaaac ttttgaccat
    16201 gtaccagaac gatttgcttc atttgcaaag aaataccagc aagaattgat tttcgaagaa
    16261 gaaaagaagc aagctatttt taaactcagt caacttatga aagaacatcc agtcattact
    16321 tttgtttatg gtgcaaaaaa tgaacatcag aaccatgctg ttttattgaa aaaatttatc
    16381 gaaatggagg catgcaaatg aaaataggga tcattggtgc cggacctaga ggattaagtg
    16441 tagcagaaag gattctccat aataatcagg acagacattc tcttgaactt tttcttttcg
    16501 atcccaatgg tcctggaggt cgagtttggc ggttgaatca gccaccggag ttattaatga
    16561 attctgtttc tcaacaagtg acgcttttta cagatgaaac attgtccagt ggaggattgg
    16621 taacacaggg acccaacttg tttcaatgga gtaaaggacc tgctacagat tatatcgaac
    16681 agacggtaac caaaaataag caactaatta tagaagaaat caatcgtctg gaaaaagatc
    16741 agcaatctac tcgatgttta tacggactct atcaacgctg gtttttctca aagttgcaag
    16801 aagaatttcc agcttctatt cagtttgttt attctttagt aaaaaaagta agcaaaagaa
    16861 aagatggtta tcttttacag acagatcagc aaagctattc tatcgatcag cttgttttag
    16921 caagcggtca ctgggaaaat gatgagacag aagaagaaag agtgtgggta gactacgcaa
    16981 agaaaaatgt tttactgtat cagcttcctt ctaatcctgc agatgtagat ctttcgaaga
    17041 taccgcctaa agaaaatgtc tttttaagag gtctaggctt aagttttttt gattatgtcg
    17101 gattgtttac agtatcaaga ggagggcgct tcgagaacga aaagggaaaa ctggtctatc
    17161 atccatcagg caaggaaccg attgtataca gtggatcaag aagggggctc ccttattacc
    17221 ctcgaggaag aaatcaaaag caaggcggag caatggcatg gccacggtta ctaacaaatg
    17281 aaaacctgga gcaatggcat cagtcgggat ttttgactgg cgaaacattt tttgactatc
    17341 tgaaaaaaga cgttgagttg ttttattaca agaaaatcat cgaagaatat cacttgccga
    17401 ttccaaagaa aaattttgag catgattttc tctccctctc ttcagaggag tgtctcggaa
    17461 agcatccagc actaatccct tatagatggg attggtcgta tttagaaact cctcttgcct
    17521 atcagaatga gcccttttca gaagctagcc gaatatttat tgaagggcag ataaaagaag
    17581 cggaaaaagg caattgcaca ggagcatttg cttcaacatt tgatgcatta aaagactggc
    17641 gtgatccaat ccggcaggct attgaatggc acgttttttc tgctaaggaa tacaaagaaa
    17701 tactatgggg atggtttact catctaaata gctttttaac gatcggtccg ccaatcattc
    17761 ggacgaaaga attagcagca ctgatagatg cgggtatttt tcatttgatc gaaccgccat
    17821 ttgatattca gcaaaagcac aaccgctatg ttatttctct acaagataaa gaaatcagca
    17881 gtcgctattt gattgaagct cgattgccac aaacaagctt ttctcgttca agtaatcctg
    17941 tcataaaaaa tttgaaggaa aacaaacttg ttcgcccttt ttcattttca gataatacag
    18001 gaagttatca ttcagaagct atagatgttg agcgaggtac gaatcgcgtg attgatgctt
    18061 caggggaaat ccagtctgac ttttattgct atggaatacc tgttgaaggt gtggattggc
    18121 taactgcagc agtagctaga ccttatacag atccatggaa tctgagacag gcagataaaa
    18181 tagcacaact tatattgact gacaaataaa aatacgcatg atgacttatc tgttttttag
    18241 aaagtcttca tgcgtatttt taaataatta ttaacgagaa acgcgtttac gcattgcttt
    18301 tttacggttt tcttcgatca ttgcttcttt ttcgtcaatt ggatcgatca ctgttttttt
    18361 cacagaagca accaaaccag caacggcagc ggctgttgcc agacttccta ctaatattcc
    18421 agatacaaa
//
LOCUS       DAJSKS010000049        18226 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJSKS010000049 DAJSKS010000000
VERSION     DAJSKS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.050"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            31..1692
                     /locus_tag="ON396_001405"
     CDS             31..1692
                     /locus_tag="ON396_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3113496.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1762..2109)
                     /locus_tag="ON396_001406"
     CDS             complement(1762..2109)
                     /locus_tag="ON396_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCR3113497.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2193..3134
                     /locus_tag="ON396_001407"
     CDS             2193..3134
                     /locus_tag="ON396_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3113498.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3135..4178
                     /locus_tag="ON396_001408"
     CDS             3135..4178
                     /locus_tag="ON396_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3113499.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4184..5254
                     /locus_tag="ON396_001409"
     CDS             4184..5254
                     /locus_tag="ON396_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3113500.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5244..6197
                     /locus_tag="ON396_001410"
     CDS             5244..6197
                     /locus_tag="ON396_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3113501.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6209..7219
                     /locus_tag="ON396_001411"
     CDS             6209..7219
                     /locus_tag="ON396_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3113502.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7504..8325)
                     /locus_tag="ON396_001412"
     CDS             complement(7504..8325)
                     /locus_tag="ON396_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR3113503.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8422..8700)
                     /locus_tag="ON396_001413"
     CDS             complement(8422..8700)
                     /locus_tag="ON396_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113504.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            8968..10977
                     /gene="metG"
                     /locus_tag="ON396_001414"
     CDS             8968..10977
                     /gene="metG"
                     /locus_tag="ON396_001414"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR3113505.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11456..12043)
                     /locus_tag="ON396_001415"
     CDS             complement(11456..12043)
                     /locus_tag="ON396_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113506.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12218..13540
                     /locus_tag="ON396_001416"
     CDS             12218..13540
                     /locus_tag="ON396_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3113507.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13695..14471
                     /locus_tag="ON396_001417"
     CDS             13695..14471
                     /locus_tag="ON396_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCR3113508.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14464..15033
                     /gene="rnmV"
                     /locus_tag="ON396_001418"
     CDS             14464..15033
                     /gene="rnmV"
                     /locus_tag="ON396_001418"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCR3113509.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15026..15910
                     /gene="rsmA"
                     /locus_tag="ON396_001419"
     CDS             15026..15910
                     /gene="rsmA"
                     /locus_tag="ON396_001419"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR3113510.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16361..16990
                     /locus_tag="ON396_001420"
     CDS             16361..16990
                     /locus_tag="ON396_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113511.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17114..18223)
                     /gene="ugpC"
                     /locus_tag="ON396_001421"
     CDS             complement(17114..18223)
                     /gene="ugpC"
                     /locus_tag="ON396_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCR3113512.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT         5945 a         3296 c         3738 g         5247 t
ORIGIN      
        1 tctgaaaatt ccaataaagg ggtacgtaaa atgaaaaaga aatttacatt tggtttggtt
       61 gcggtatgcg gccttgtact tgctggatgt tatggcggca gttcagccac aagcagtagt
      121 tcagcaaaaa gcggttcagc agatggagga ggcgtattta acttagtcgt gcctcaagaa
      181 atgcctacag ctgacttatc agttgcaaca gatacaatca gttttacagc attgaataac
      241 gtttatgaag gaatttaccg tcttgatgaa gacagcaaac cacagccggc aggagctagc
      301 gagttggcag aagtcagtga agacggatta acttacaaaa tcaaattacg agaagacgca
      361 aaatggtcaa atggtgatcc tgttacagca gcggattacg tttatggctg gcaacgaaca
      421 gtagacccag ctacagcttc tgaatatgct tacctgtatg cgccagttga aaatgcagaa
      481 gacattacag ctggaaagaa agataaatca gaactaggga tcaaagcagt tggtgactat
      541 gaattagaaa tcaaattgac gaaacaaaca ccatatttcc aatatctgct tgctttccca
      601 tcatttttcc cacaaaaaca atccgtagtg gaagaaaatg gggatgccta tgcatctgca
      661 agtgataaag ctgtttataa tggaccattc acattagaag attttgatgg accaggaaca
      721 gatacagaat ggacttataa gaaaaatgat cattactggg acaaagatac agtaaaactt
      781 tctgaaatca aagtcagtgt agtaaaagaa tcttctactg cattgaactt attcaaagat
      841 ggccaagcag atgacgtgat cttgtctggt gagttagcac agcaaaacgc aaacgatcca
      901 gcttatactt ctgttaaaga agcgcggaca agctatatcg aattcaatca acgtgaaaag
      961 aattcaccat tcaacaatgt gaacctaaga aaagctattt cttattctat cgatcgtgaa
     1021 gctttagtaa aacaagtact aggtgacgga tcagttgtct ctacaggatt gatcccatca
     1081 gatatggtaa aaagtccaac aacgaacgaa gactttgcaa aagaagctgg aaaattagtt
     1141 tcttatgaca aagataaagc aaaagaatat tgggaaaaag cgaaaaaaga attgggcgtt
     1201 gattctttag aatttgattt gatggcttct gatgatgatt catcgaaaaa agtaatcgaa
     1261 tatgttcaaa actcaatcca agaaaacttg gatggcgtaa cagtcaaacc aacacctgtt
     1321 ccattctctg tacgtcttga ccgctcaact tcgggtgatt ttgacgcagt actaggcgga
     1381 tgggctgctg actatgcaga cccaagcagc tttactgatt tgtttgtcac aggtaattca
     1441 tacaaccgtg gtcgctggag caatgcagaa tatgacgaag cagttaaagc agcttcatca
     1501 acagacgctg gcgatgaaca agcccgttgg gatgatttcc aaaaagcaat caagatcatc
     1561 tctgatgata tgggtgtcgt tccggtgtac caaaaagcag aaggtcacct agtgaatgaa
     1621 aaaatcaaag gtatcgttca ccatgcggca ggcgcttcat gggattacaa atggacatac
     1681 gttgaagaat aatcgttaat accgtgagca tgagaaaaaa cgaggctgcg gcaaatgtcg
     1741 caacctcgtt ctcatattca cttatcctaa caaagaaaaa agcatgaaaa tagcagaaag
     1801 agcaattagt attccggcaa tgatcagcca aaagctgtac ataccgtagc tggcagacga
     1861 gagcgaggaa aattctggtt ttttctttcg gttccttgtt cgtgcaagat ggaccgatcg
     1921 ctgaaaatga tcgaagaaac ccgaagaaaa gacccataaa aaaccgccga taatcagaaa
     1981 cggcaatgac cataagaata gatcattaga taattgaatg agtgtaaagg aatggttgat
     2041 gagatttttt attaaaataa ccagaatcag tagaaaagct actgcgtagg ggatagattt
     2101 ttttttcatt tgattgctcc tttatttttg gttcattatc taataatgta gcgaaacatc
     2161 tgtcagaagt caaaagctag ggggaaacat tagtgaatag ttatatcaaa tatgtattaa
     2221 aacgtgtctt tttcatgatc atcacgcttt ggttaattgc aacaatcacc tttttcttga
     2281 tgcaattgct gccgggcacg ccatatacga atcaggaacg tttgagtcct gaaacaatcg
     2341 agatgctaaa taaacaagtc ggtttggata aaccagttat cgtacaatat gggatttatc
     2401 tttctaattt gcttcaagga gattttggaa tttccttcca atttaaaaac cagccagtcg
     2461 cacatttgtt agctggaaga atcggaccat ccttgcagtt agggttacag gcaattatct
     2521 ttggaacagt cctagggacg attttaggaa cgatctctgc catgaagcaa aatacatggg
     2581 cagatacatc ttctacactc gtagcgatat taggtcgttc cattccaaac tttgtatttg
     2641 ctgttttatt acaatatatc tttgcaatca aattacaagt tttgccaatc gcaaaatggg
     2701 atggctttgt gtacacaatc ttaccaacaa ttgcgttggc catgtcgccg cttgctgact
     2761 ctgctcggtt tatccgtacg gaaatggtgg aagtactgca tagtgattat gtggaattag
     2821 caaaagcaaa aggattgagc cgttgggaaa tcgcctttaa acatggtctt cgaaatagtt
     2881 tgattccatt gatgacttta ttaggaccgt tagctgtagc attgatgacc ggatcactgg
     2941 tggtagaaaa tatctttgct attcctggta tcggggaaca attcgttaag tcaatcacaa
     3001 caaatgatta tcctacgatc atggctgtaa cgatccttta ctcatttatg ctgatttttg
     3061 ttattttgat cgtggatcta ttgtacggtc tagttgatcc aagaattcgt gtttcagaag
     3121 ggagccgagg ataaatggaa atgataccaa agcgttatga aacagttgca gatatccctg
     3181 cggatgaatt tcttccatta caaaaaaata ccgaagaaga acgtgaacag attactgcac
     3241 cttctttgaa ttttatccaa gattcatggc gccgtttaaa gaaaaacaaa gctgccgtta
     3301 tttcaatggt tttactattg gtcatcattt tgatctctgt gatcacaatc tttgtttctc
     3361 ctcatgatcc aactgcccaa aacgtggctt atatcaattt gcctcctcgt attccgggaa
     3421 tcaatatcga tggattgaat ggaaaagcaa tggttggtgg tgaacttgtt gataaatatg
     3481 ctcaagcaaa cgtcccttca aacgtaaatt tctttttagg aacagacggt ttgggacgtg
     3541 atgtattaag ccgcttattt atgggaatga gaatctcgtt gctgatcgca tttattgctg
     3601 cattactaga tgtaacgatt ggtgtagctt acgggttggt ttctggtttg ttaggcggac
     3661 gtgtcgataa cgtgatgcaa cgttttttgg aaatcttatc aggaattcca aacctcgttg
     3721 tcatgatttt gatgttggtc gtttttgaac caggaatttt ttcaatcgtg gctgctatgg
     3781 ccatcacaaa ctggatacca atggcacgta tcgttcgtgc acaaacaatg aaattaaaag
     3841 atcaagaata tgttttagct ggattgacat taggtgaatc aagaatgaaa attgcattca
     3901 aacatatttt gccgaatatc tctagtgtca ttattattca aatgatgttt agtattccat
     3961 ctgctatttt cttcgaagcc tttttaagct ttatcggttt aggtcttacg cctccgtcag
     4021 cttcattagg aacgatgttg agcgaagggt acaagacatt cctgtatctg ccttatctat
     4081 tatggattcc ggcagtaact ttatcggtta tcatgattgg ctttaactta cttgctgatg
     4141 gattgcgtga tgcctttgat cctaaaatga aagagtgaag acgatgacga aaattttaga
     4201 agtgaaagac ttagaaatct cttttgatac gtatgctgga aaggtacgag cgattcgcgg
     4261 tgtcaatttt catttgaata aaggagaaac attggcgatc gttggggaat ctggaagtgg
     4321 aaaatcagtg acgaccagaa gcatcatgcg cttgctgtca agcaacgcca atatagatgc
     4381 tggtcaaatc ctctttaaag gtaacgatat cgttggaaaa tcagaaagag agatgcaaaa
     4441 aatccgtggt aaagaaatag cgatgatttt ccaagatcca atgacctctt tagatccgac
     4501 gatgcccatt gggaagcaag tcgctgagtc ccttagaaag cataataaag tttcaaaaaa
     4561 agagggacaa aaagctgcat tagaattgct taaattagtt ggaatacccg aagctgagaa
     4621 acgtatcaac agttatccgc atcagttctc aggaggtcag cgtcaacgga tcgtgatcgc
     4681 gattgcactg gtatgttatc cggaaatcct gattgctgat gaacctacaa cagcgcttga
     4741 tgtgactatt caagcacaga ttttggagct gctgaaagaa attcagacaa aaatcgatac
     4801 atcaattatc tttatcactc atgaccttgg tgtagtagcg aatgtcgctg atcgtgtcgc
     4861 tgtcatgtat ggaggaagaa tcgttgaagt tggaacatcg gaagaaattt tctacaaccc
     4921 gcaacatccc tatacttggg gactgcttgg ttcaatgccg acattagata gtgcgaacga
     4981 tcgattgtat gctattcctg gttctccacc ggatttattg aacccgccaa aaggagacgc
     5041 attctatcca agaaatgaat tcgcgatgaa aatcgatgca gaacaagaac caccattttt
     5101 tgaactatca aaaacacaca aggcagcaac ttggctactt gctccgcagg cacccaaagt
     5161 gacaccgcca gaagaaatcc aacgtcgctg ggctatcttt aaagagaaac aaaaacatac
     5221 ttacggtcaa ggaggaattg ctgatgccaa ataaactact tgaagttaaa ggattgaaac
     5281 agtatttcaa tgtaggaaaa aagaatgaag tacatgctgt aaatgacatc agctttcaca
     5341 tttatgaagg agaaacattc ggtttagttg gagaatctgg aagcggaaaa tccactactg
     5401 gccgtacgat cattcgcttg aatgaaccta ctgatggtga gatattattc gacggtcaag
     5461 atataacgaa gatcaaagat aaaaaaggtt tgacgaaatt ccggcatgat gttcagatga
     5521 tctttcaaga tccatacgct tcgttgaatc ctcgtatgaa agtcagagat atcattgccg
     5581 aaggaattga tgttaatgga ctagctaaat catctgaaga gcgtgcaaaa aaagtcgatg
     5641 atctattgag aactgttgga ttgaatccaa gccacggtac tcgttatcct catgaatttt
     5701 caggtgggca acgccagcgt attggtattg cacgagcatt ggcagtaaaa ccgcgtttta
     5761 tcatttgtga tgaaccaatt tctgcactag atgtgtcgat ccaagcccaa gtggtcaatt
     5821 tgttgcaaga tctccaaaaa gaacatcaat tgacctatct atttattgca catgatttgt
     5881 cgatggttaa acatattagt gaccgaatcg gtgtaatgca taacggcttg ttattagaga
     5941 tgggaacaag tgatgaaatc tataatcacg gtgttcatcc ttatacagaa agtttgttat
     6001 ctgctattcc tctaccagat ccagaccatg agagaaaacg cagaagaatc aaatatcaac
     6061 ctgaaccaga tgacggacaa gtacgtcaac ttcgtgaaat cgcaccagaa cattttgtct
     6121 atgcaactga gcaggaagtc ccatattacg caaaaaaact aaaacgacaa aaggaatctc
     6181 ttctggttgc taactaggag ggacatagat gctaaaagct tttaaattcc gtatgtatcc
     6241 gacagaagaa caaaaacagc aattgattcg tacttttgga tgtgctcgat ttacctataa
     6301 ccatttgctg aaaaagcggc agaaaagttg gcaacaaaca ggagtagcca atttttcgct
     6361 cacacccgct acattaaaaa aagaataccc attcttaaaa gaagtagaca gtcttgcttt
     6421 agcaaatgca cagcttaatt tggatcgggc ttttcgcaat tatttcaagg gacgtgccag
     6481 cttcccaaag cttaaaacga aaaaaagtat gtggcaatct tatacgacca acaaccaaac
     6541 ccgtacgatc tatctggaaa atggctactt aaagttacca aaacaaaaag aactaatcaa
     6601 gataaatcag catcggccag ttgaaggatc gattcgctcc gctacgattt ctgctcgata
     6661 taatgaagaa ttttatgtcg cactactttg tgatgtttcg ccagtaaaaa aagaatcttt
     6721 agctaaatgg atcgggatcg cctatcatcc gaagacattg atccaaacat ctcgacctct
     6781 agaagtaact ttaccaaaat ttcaccagac agaagaaaag ctgcaacatg cacagcggaa
     6841 attgaatgtg aaagtccgct ccgctcacca tagaaagatc aggttagatc aggcaagcaa
     6901 ttatcagaaa caaaagagaa aagtaatgga tctttatctg aaacaaaaga accagcgaga
     6961 agattatctt gaacaattat ctgggaaatt ggtcaaacaa tatgactact tatttgtaga
     7021 atcttttcca aaagaagagg cacatgctga tttttctatt cacgactggc ataaattaat
     7081 aacaaaattg cgttataagt cccaatggta taataaaaaa tttcttttga tcaataccga
     7141 cggagcagaa gaaagcaatt ccgtaagaaa gagccaagta gtagaaaaaa tgggcagaca
     7201 ttcggttatc aagggataaa aaatgaatga aactgatagg agcgttaggg ttagccttgt
     7261 taataaaaag tcttaatgac ttttgtttct aggaattttt cttttgaaga aaagctcact
     7321 aattatgaca aaccaataaa acaatagcag aaaaagttcg tacgagcttt ttctgctatt
     7381 gttaattttt tattcagaaa gtttacgaat acatcaaaat tctgaaacaa aaaacgtaac
     7441 tcacaaagag ttgtttttgc gttttttctt gaatgattag gtctgaatct tttgctcaca
     7501 accttattca tggtgccccg gcttgagctg atagattttt gaaggacggc cgattcctac
     7561 aggacgttca ccgatctcat cgaaatattg gagcatagct ttcttaaaat tggaatgatc
     7621 gatcgtttta taatcgatcc ctaagaattt agcaaatact tttcgagctt ccgtaatcgt
     7681 gaaatcttta cctaaaactt gtagaacttg cggctcgtgt tccattttat tgactacacg
     7741 gttgaatgct ttcaagatga tttgactatg gtcaaaagcc aaagtatctt tgccagaaga
     7801 ctctccagtg actaaatcaa gcgagatagc cacttctccg ctagataaat ggatcttgtt
     7861 actatgacgt tctagtgtga accagcgtac ttccttggca tcatctccgg cgattagcgg
     7921 ttcttctcca ataaaggcta agtagctgac tgtgacgacc cagcctcgcg gatctcgatt
     7981 aggcgtactg aatgtatgca gttgctcaat attttctttt gaaataacca caccagtttc
     8041 ttcctttgtt tctcttaaga cgctttctcc agttgactca tccttttgga caaagccacc
     8101 aggcaatgcc catgaatttc gataaggatg cccttttctt tggatcaaca aaactttcag
     8161 ctgatcttct tctttgttat agcacatcag tacgatatcg acagtcaaag agggcttgtc
     8221 atattctgga cgttcctgtt tttgatacca gttaagaaat tcttcttcac ttgctttatg
     8281 ttcataatat aatttttctt cttctttaga agcaaaacgg ttcatctgac cacctccatt
     8341 gacagtaaaa gtacttttta ccttattgta gcagactctg aaaaaaaacc aaggatttac
     8401 ttgaaaaatc taggttatca tttaaatatg aaaaataaat ggaagtatga gattgaataa
     8461 aaagaaaagc agataaaacg gcaatacttt ataccgctct tcacgaatgg caaagaagcc
     8521 acaaataacg atatatacgt caatgataaa acgcaaaatc caagctgctg tcataaaact
     8581 gttcaaatgc ccatggaggt taaaataacc atacatacga atataaaaat agctgaatat
     8641 taaatgtgct aatgaaagaa aagcaatact aagtgtaaaa aaaagatgtt tcttctgcat
     8701 gattgttcca gtcccttact aagatagata gtctttatta taccacgtgt gaggggagaa
     8761 ataaaaagcc gttttttagt agatatgaaa caaaacaaaa acccgcattt attcaaccta
     8821 ttcatccttt taaaggatag attgaacgta gatagaagtc gtttatccag tctttttcat
     8881 tttgaaaaaa agctaacagc atgctttttt tatggtatac tagcaaggaa acgaattcgc
     8941 atcatgcgat aaaggaggaa gagccccgtg gcagaaaagg aaacttttta tatcactacg
     9001 ccaatctatt atccaagcgg caaacttcat atcggcaact cgtatactac gattgcatgt
     9061 gacgcaattg ctcgttataa acggttgatg ggctttgatg tgttttatct cactggagtg
     9121 gatgaacatg gtcaaaaaat cgaaacaaaa gcagaagaac taggtgtaaa accgcaagaa
     9181 tatgtagata aaatggcggc tgatgtcaag aaattatgga aaacgcttga tatcagttat
     9241 gataaattca ttcgcacgac agatgattac cataaagcag ctgttcaaaa aatatttgat
     9301 cgtttattag aacagggaga tatttactta ggtgaatatg aaggctggta ctctgtttct
     9361 gacgaagaat tctttacaga aacacaactt gcagaagtgt acaaagatga cgaaggtaac
     9421 gtgatcggtg gtaaagcgcc aagtggacac gaagtagaat tggtaaaaga agaatcatac
     9481 ttcttccgca tgagcaaata cgcagaccgt ctattggaat actatgaaga acatcccgaa
     9541 ttcatccagc cagaatctcg taaaaacgaa atgatcaaca actttatcaa accaggatta
     9601 gaggatcttg cagtttcacg aacaactttt acttggggag taccagtcaa gagtgatccg
     9661 aaacatgtgg tgtatgtatg gatcgatgca ttatccaatt atattactgc actaggatat
     9721 ggctccgaag atgaaagtct attcgaaaaa tactggcctg cagatgttca gatggtcggt
     9781 aaagaaatcg ttcgctttca tacgatctat tggccaatca tgctgatggc ccttgatttg
     9841 cctctaccta aaaaagtatt tgggcatggc tggctattga tgaaagacgg aaaaatgtct
     9901 aaatcaaaag gaaatgtcgt ttatcctgaa atgcttgtgg aacgttatgg tttagatgca
     9961 ctccgttatt atttgcttcg tgctgttccg tttgggtcag atggtgtctt tacgccagaa
    10021 gattttgttt cccgtgtcaa ttatgatctg gcaaatgatc tagggaactt attgaatcga
    10081 acgattgcta tgatcaacaa atattgtgat ggactcgttc ctgactatgc ctcacttgtc
    10141 actccatttg atagtgaact gtctacgacg gctgctaacg ttgtcggacg ttaccatgat
    10201 gcaatggaaa aaatggaatt caatacagca ttggctgaaa tctgggtgct gatttctcgt
    10261 gcaaacaaat acattgatga aacagaacca tgggtattag caaaagacga agaaaagaaa
    10321 gctgaattga atagtgtcat gatccattta gctgaatctt tgcgtatcgc agcgatatta
    10381 cttcagccaa tcatgacaga aacgccagag aagatcttca atcaactagg attggactct
    10441 gaaacaatga atcttgaagg attacacttt ggcgaatttc cttcaggaac aaaagtagtt
    10501 gcaaaaggta caccaatctt cccacgttta gatatggaag aagaagttgc ttttattcaa
    10561 gaaaaaatgt ctgaaggtac tcaaacaaac gaagatacag tcaaatggga tccagaagaa
    10621 acagaacttg tttcaacgaa agaaaaacaa atcaaatttg atgtatttga aaaagtagaa
    10681 ctaaaagtag cagaagtcat caactgtcaa aaagtagaag gggcagataa gttgctgcaa
    10741 ttccgactag atgctggaga cagccaagac cgtcaaatct tgtcaggaat cgctgaattt
    10801 tatcctgatc caagcgaatt gatcgggaaa aaagtcgtca ttgtagcaaa tctgaaacca
    10861 cgaaagatgc gtggacaaat cagtcaagga atgatccttt ctgcagaagc accagatggc
    10921 tctttacaag taatcgaagc accaaaatcc atgccaaatg gctcagaaat cgcatagaaa
    10981 ttcaaagggg gtatgacact agttgtgtca tacccccttt ttccgaaatt tgagaagcaa
    11041 ttttcgtaaa ttccttctta ttacttgaag ccaatcatat aatacagaaa cagagaacta
    11101 tccaaaataa atcctagaaa aagaagtacg agcttcgctg atttttgtta atttagaata
    11161 atactaatag gaatcgtaaa aacagaaaaa aagcataatg ataaagggtc gggcagattt
    11221 taattgattc atctggtact cttttttatt gtccttacat tcataaagtt tgaatgcaat
    11281 gactttatta catctatatt tatatctaac agtagcgttg atgtgttaat tataagttct
    11341 atcactttat gttcaattag tttgtgctct tatatgtgtg attgaaaata tagataaaaa
    11401 aacaatcatc ggctttgact tcgatgattg ttttctttat tttgtaaaaa tcctatcact
    11461 tgattttatg gtaacctttt atttttgtct tcaaacggct tggtaagaga gcagtaagca
    11521 gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg tccgatacca taatgttgat
    11581 agagctttgt caacatcacg tgtaacaaga tcaaccctaa aagcaaggag ccagctataa
    11641 accaatgatt atcccaatgc atgacttcac taagtgccat cactccaaaa ggatagaaat
    11701 aaaaatagat cccgactaag gtcatccgcc agttctttgt aaatgttccg atattcaata
    11761 aaaagaaaat cgctgaaagc aaaataccga taggcggaaa aagcccaacc aaaaaggaat
    11821 acaacatccc ccacaagatc atcacaggac ctttgatcaa tgcaacgatt gcgatgaaca
    11881 aagtcagcaa tattagtttt tgtgataacg ttgcaacagt cattaatcca aataaaatta
    11941 atgtgaacca atgccaatgg gactgctgtt gttctttacg ggaaaaatcg acccggttgg
    12001 ataaataatc tgctaatttc ggataagaac gcttaaaatc cataaactgt tcctccttcg
    12061 tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa tacatccgtc ttaagaggga
    12121 gtttttcact gaaagtggag aaaaaagaat ggtaaattaa aatattctca gttatactga
    12181 aaattatacc aaatttcaaa gaagaaagag gatgggaatg aaaggcaaac taaaacgaga
    12241 aatcaatctg tttggcgcat tagctaccgt tatgggaacc gtgatcggtg caggcgtctt
    12301 cttcaaaact gctgctgtta ctgctagtac tcagtctaca agtttgacat tgctagcttg
    12361 gcttttgggt ggtttcttaa cgatctgtgc tggattgaca gtggctgaat tagctacagc
    12421 aatcccggaa actggtggag ctgtaaaata tattgaagct gcgtatggaa aattgcccag
    12481 ctttttatta ggatgggcgc aaagcttgat ttactttcct gctaatattg ctgcattatc
    12541 gattatcttt gcgacacaat tgacgaactt attacaatta tctaccgatt atcttttgtt
    12601 gattgcagtg atcaccgctg tttctgtgac agggttgaat ttgttaggga caaaagtcgg
    12661 tgcttctgta caatcagtga cgttgattgt taaattaata ccaattgctg tgattgtcat
    12721 atggggatta ttgaccccag gacaaggaac tgtccagtta tttccaatcg aagctggaaa
    12781 agatgttact tttgtcgaag gattgagcag tgcgttgtta gcaacgttgt ttgcttatga
    12841 cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa cggccggaaa aagatttgcc
    12901 taaagcaatt attcttggtt tgagttttgt aacagtcgtt tatctattga tcaattttgt
    12961 ttttttgaaa actttgccga ttgatcatct tgccggtaat ttgaatgcag cttctgaagc
    13021 atcagacgtc atttttggag gaatcggtgg aaagctggtc actattggga tcttgatctc
    13081 ggtatacggt gcattgaacg gttatacact aactgggatc cgcgtgcctt atgcgatggc
    13141 tttagaagac gagctacctt tcagtaaaca gctgacaaag ctttcaaaga agtttacggt
    13201 tccttatgtt cctgcggttt tccagttagc agtagcttgt attatgatga gccttggttc
    13261 tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg ctattcagtt tactgatttt
    13321 tatcggtgtc tttattttaa gaaaaaaagc gccggaattg ccacgtccat ataaagtacc
    13381 actttatcca atcgttccga ttattgcgat tctcggtgct attttcattt taggtatgac
    13441 gatgatgacg caaacgaaat tagcattgat cggtataggt gttaccttga taggtatccc
    13501 agtctattat caaaaaaaga aaaaacgctc agaagagtaa aatatttcaa caacagacag
    13561 aaaaagtacg aactaggtcc tctagatctt cgtgcttttt ctatttcaat aaaacaagtt
    13621 cttctatttt gatttttgct acttctgcaa gtagtgctta tgtgctaaag taagaatgaa
    13681 aaggagagat aaacatgata tttgattctc atacccattt aaatgctgaa cagttcaatg
    13741 aagatattcc agaaacgatt cagcgagcac aagaaatggg ggtaacgaaa atggcggtcg
    13801 tgggcttcga tacgccaacg attgaaaagt ctttgcaatt aagccatgac tatccaaata
    13861 tatacagtat tatcggctgg catccgacag aggctggcag ttatacgaaa gaaatcgaaa
    13921 ataaattaca agaacaattg actttaccaa aggttgtagc attaggggaa attggcttgg
    13981 attatcattg gatggaagac ccgaaagaag tccaagataa agtatttcgc agacagattg
    14041 ctatcgcaaa agaaatgaac ctaccaatca gtattcatac tagagaagca cttgaagaca
    14101 cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg tggaatcatg catagtttca
    14161 gtggtgatta tgaatgggca aaacgatttc ttgacttagg gatgcatatt tcatttagcg
    14221 gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc tgcagcaaat gtaccaatcg
    14281 aacgattact tgtggaaaca gatgcccctt atctggcacc agttcctttt agaggaaagc
    14341 gaaatgaacc aggctatact cgttatacag tagaaaagat tgctgaactt cggaatcttc
    14401 cgacagaaga agtggctgcc caaacatgga caaatgcgca ccgattattt ggtctaagcg
    14461 aacatggata agatgaagat ccaagagatt atagtcgttg aaggaaaaga cgatacaaga
    14521 aggctgcggg aagtagttga tgcagatacg atagaaacaa tagggtctgc aatcaatgaa
    14581 gagatcctga tgcaaattga acatgcccaa gagactcgtg gagtgatcgt ttttaccgat
    14641 cctgattttt caggagaaaa aataagaaaa atcattatgg aagctgttcc tcaggcaaaa
    14701 catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa agggaagcct tggtgtcgag
    14761 catgcaagtg atgctgctat tttagaagca ttgaaaaagg tcgtgacgcc agacatttcc
    14821 caaacggctg tttctgaaat cactagacaa gaccttttag actatggttt aatagctggg
    14881 gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt tgcgcatcgg ttacacgaac
    14941 ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta cgccgaaaga actcgcccaa
    15001 gcaatgaaaa tagtggagga acaacatgag tgaatatcgt gaaattgcga caccctctag
    15061 aacaaaagag attttattga aacatggttt ttcatttaaa aaaagtttag gacaaaattt
    15121 tctgactgaa ccaaatattt taaggaaaat cgtagaaact gcgcaaatag atgaacatac
    15181 gaacgtgatc gaagttggac caggaatcgg tgcattgacg gaacaattag ccaagcatgc
    15241 aaaacaagtc gtagcatttg agatcgatga tcgtttgatc cctgtattgg ctgatacgat
    15301 gcaaccctat gacaatgtga ctattgtaca tcaagatatt ttaaagaccg atttgtcaac
    15361 agctgttaga gaaacatttc atgaggagct tccgttaaaa gtcgtagcga atttacctta
    15421 ctatattacg acaccgatca tgatgcattt tcttgaatca gatttggttg tggatgaact
    15481 agtcgtcatg atgcaaaaag aagtagctga tcgtatctct gctgaaccag gaacgaaagc
    15541 ttatggttca ttatcgattg ctgttcaata ttatatggaa gccagtttag cattcatcgt
    15601 accaaaaaca gtatttgtcc ctcagccaaa tgtagattca gcgattttga aactgacaag
    15661 aagagatata ccggcagtcg aagtgacaga cgaaaaagca tttttccgat tgacaaaagc
    15721 ggcatttcag caaagaagaa aaacgctttg gaataacttg cagcattcgt atggcaaaga
    15781 tgaccagaca aaagcatggt tagcaaaaag cttggaaaca gctggaatcg atccaaaacg
    15841 tcgtggggag actttgtcat tacaagaatt cgccgcatta agcaatgcga tgtcagaaaa
    15901 tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa agctgtcctg catcaagtaa
    15961 taagaacagc caaacaaaaa tagttcctca tgtttttcgt ttgtttgggc ttattaccgc
    16021 agatgcaagc ttttgaacac cgtttaagaa agaggttgtg ggcctgccgt ttaactctaa
    16081 gtcacaagga acaatttcaa aaaacagctt ctcatgtttt tgagttagtt ggtcttatta
    16141 cttgatgcaa gcttttgacc actattatgc cgaacaagga gatgtgacac aaattttgtc
    16201 acatctccta tttttgtttc ttgatcaaaa aattcttata cttgatactc acactcattt
    16261 tgtatggatt tagaaaagaa tatgtgtctt gacagttatt agtaagtgag ttatactaag
    16321 tcgacattta tgtgtcaaga catattagga gagagaaaaa atgagaaaaa attcagttcg
    16381 aaaactaacg atttctgctt tattgattgg gatgggggtc attatcccaa tggtcatgcc
    16441 taaaatcatg attccaccag cttcctttac tttggcgtct catgtgccat tatttattgc
    16501 gatgtttttt acaccaggag ttgcagtggc tgttgcgcta ggtacgacat ttggtttctt
    16561 tttaacaacg ccggttatca tcgcattgcg agcattgtca catcttgtgt ttgcgttgat
    16621 tggggcttgg tacttgcaaa agcatccgag tatcgtttta aaaaatggac agtttacttt
    16681 tgctaattgg cgtttccaag ggttcaattt tgtgatcggt gtgattcatt ctttagcaga
    16741 aatgctagta gtcagcatct tttactttat tggtaatatg cctgaaacat attattctca
    16801 aggatatttt tatacagtat tcatcttgat ggggattggt gggcttatcc acagtttagt
    16861 tgattataat attgcttatt ttatagcagg aacactcagc aaatattttg atatccccgt
    16921 atttacggca gcaaaaaaag aaaatcggaa agagtcgatt caagggtacg cgaagaaaac
    16981 gacatattag acatgatctc tcaaaaaaaa aactctcatc tagtaatgga taataaatcc
    17041 tctaagatga gagttttttg tcctaaaaag agctgcacca acatcttctg gcacagctct
    17101 aacagggggc aatttatgga agtttgatga cttgttctgt ttctttatca aagaagtggc
    17161 ctttgctgat gttgaaggca aggtcgacta tttcgcctgg tttatggaaa tctcttgcat
    17221 ctacttttga gatgaactca gtagaaccag tttttgtgta aagcatcgtt tcagctccaa
    17281 gtaattcgga aacgacaact tctgatttca ctacggcttc aggtgaagct tcaagggcta
    17341 cttgttcact gtggatgtct tctggacgaa taccgaagat aacttctttt ccttcataac
    17401 ctttttcaac taatactttg ttttttcctt caggaatacg aagcttaagc ccatgtccat
    17461 ctgtgataac accatttttc aatgtcacat taaagaagtt catggcagga gatccaataa
    17521 agccggcaac gaacatattg acgggtgtat cataaacttc tttcggtgta ccgatttgtt
    17581 ggataaatcc gtctttcatg ataacgattc ggtcagccat agtcattgct tctgtctgat
    17641 cgtgagtaac atagatggtc gttgtttcaa gacgctggtg aagtttggca atctcagcac
    17701 gcatggctac acgtaattta gcatctaaat ttgataatgg ctcatccatt aagaagactt
    17761 ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg gcgttgtcca ccagataaag
    17821 cagctggttt tcgtttcaag tattctgtca gacctaagat ctctgctgca ttgtccacac
    17881 gttttttgat gtctgcttta tcatatttac gtaatttcaa gccaaaagcc atattatcaa
    17941 aaacagtcat atgaggatac aatgcgtagt tttggaaaac catggcaata tcacggtctt
    18001 taggtgccac gtcattcatg acttttccgc cgatatttag ttctccttca gagatatctt
    18061 ccagtcccgc gatcattcgc aaagtagttg actttccaca accggaagga ccaacaaaaa
    18121 cgataaattc acggtctttg attgatagat taaaatcagt tacagaataa ttttctgcat
    18181 tgtcatattt tttatatacg tttttcagag ccatttctac cataat
//
LOCUS       DAJSKS010000050        17919 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJSKS010000050 DAJSKS010000000
VERSION     DAJSKS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.051"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            18..1358
                     /locus_tag="ON396_001422"
     CDS             18..1358
                     /locus_tag="ON396_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3113513.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1576..2019
                     /locus_tag="ON396_001423"
     CDS             1576..2019
                     /locus_tag="ON396_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCR3113514.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2523..3941)
                     /gene="nhaC"
                     /locus_tag="ON396_001424"
     CDS             complement(2523..3941)
                     /gene="nhaC"
                     /locus_tag="ON396_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCR3113515.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4344..5567)
                     /locus_tag="ON396_001425"
     CDS             complement(4344..5567)
                     /locus_tag="ON396_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCR3113516.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5598..6437)
                     /locus_tag="ON396_001426"
     CDS             complement(5598..6437)
                     /locus_tag="ON396_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR3113517.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6454..7134)
                     /locus_tag="ON396_001427"
     CDS             complement(6454..7134)
                     /locus_tag="ON396_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3113518.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7136..8209)
                     /locus_tag="ON396_001428"
     CDS             complement(7136..8209)
                     /locus_tag="ON396_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3113519.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8265..8503)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8546..8779)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8856..9527)
                     /locus_tag="ON396_001429"
     CDS             complement(8856..9527)
                     /locus_tag="ON396_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3113520.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9614..12292)
                     /gene="mgtA"
                     /locus_tag="ON396_001430"
     CDS             complement(9614..12292)
                     /gene="mgtA"
                     /locus_tag="ON396_001430"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR3113521.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            12951..13388
                     /gene="ndk"
                     /locus_tag="ON396_001431"
     CDS             12951..13388
                     /gene="ndk"
                     /locus_tag="ON396_001431"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR3113522.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13410..14276)
                     /locus_tag="ON396_001432"
     CDS             complement(13410..14276)
                     /locus_tag="ON396_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3113523.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14436..16529)
                     /locus_tag="ON396_001433"
     CDS             complement(14436..16529)
                     /locus_tag="ON396_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR3113524.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17278..17901)
                     /locus_tag="ON396_001434"
     CDS             complement(17278..17901)
                     /locus_tag="ON396_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113525.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         5152 a         3720 c         3160 g         5887 t
ORIGIN      
        1 aaagaaagag gtcatttatg gaagataaaa aattgaaaaa acaacttagc tccagacata
       61 tcaccatgtt agctttaggt ggagcaatcg gtgcaggtct atttaaagga agtggcgaag
      121 cgattgggat cgccggccct tctgtattga ttgctttttt gattggtggg ctgattttat
      181 atatcgtgat gaaaggtctg ggaaaaatcg ttttaagcgg tggcgacacg catcacggat
      241 tatctggttt gatacgccct tatctaggtg ctcattctgc tgattttact gattgggtct
      301 actggtctat gtggattatc aatatcatcg ctgaagctgt tgcagctgct tcctttttgc
      361 agttgtggtt cccgcacatt ccagcctggg tatttgtttt tatgttggct gttttaacta
      421 cgatcatcaa tctttattcg gtacggttat tcgcagaaac agaatattgg ctggcttttg
      481 ctaaaatcag tgtcattatc ttactgatca tttttgctac ttatcttgtc ggtcagcaaa
      541 tgttgggcac aggcgttttc cctacccttc agcaattgac tgaccatggt gggtttactc
      601 ctcatggtat gaagggaatc atcagttctt tgcttgtagt tatctattct tacggcggtt
      661 ctgaactgat tgctatcacg gtaagcgaag cagatgatcc taaacgtgcg attcctaaag
      721 caatcaaagg tgtcatggga agaatcattt ccttttacat cattcctttg tttttgcttt
      781 tgattctttt tccttggaat actttagcag gcacgaatgt cagtcctttt gtgatggtat
      841 ttgaaaaaat gaatatccca tttgcagcgg atatcgtcaa ttttgtgatt gtccttgctt
      901 tgttctcatc tatcaattcg ggtgtgtatg catcttcaag aattttgttc ttccgtttga
      961 aggatcgaaa aggttcctct agaaaactag ctgttttaaa caagcatcaa gtaccgcaac
     1021 gtgctgtttt attttgtacc agtgttcttt atcttggtgt agctctttct tattttgtcg
     1081 gtgacaaatt atttggctat ctggcaggtt ccctttcgta tacagtatta ttgatctgga
     1141 tcattatcag tgcggcatcc tttgtactgg cactaagaaa aggaacatta tgggataaag
     1201 gaatcagctt ttttgcatta gctgtcttag gattgatttt ccttggtatc ctgttcacga
     1261 actccattgg ggtaactgta ttgactggcc tgctttacct gttcatttat ttcagttatc
     1321 aaaaaaagaa tgatgcgttc gtattgacga atgaatgaaa tcgatagaaa attttatttt
     1381 atttatgtgg aatagatgga gctattgaag aaaggagttg tgacaaaagt cttgttgcaa
     1441 ctcctttttc cgaataaaca gtctcatgac ctcttggtaa acggtgttca aaaaccctct
     1501 gtgcaaaata attgattgtt ctccatgctc atgacatgat agaagaaagg aatgaaaaaa
     1561 aggagtttgt tttaaatgaa aaagtattgg aagtttttgt tgatgatcgg tgtttcaacg
     1621 gtagttatgt ttggtatgat gtacttgaat gtctatgcgc tcgatcatat cttcttcagt
     1681 cgtacacgag tattcatggc gttgatgatg ggtgcagtca tggcagtcat catgctgttg
     1741 ttcatgtgga aaatgtatga aaacaaaaag ataaatgtta tgatcttagc cgctagtctt
     1801 cttgtttttg ccggttcctt gtttatggta cgaagccaaa ctactgtcaa cgacacagct
     1861 tggatgaagg ccatgattcc tcatcattct atcgctatcc tggcaagtaa acgagctgat
     1921 ttaaaagatc ctgaagtaaa aaaattagcg gaagaaatca ttcaggcaca agaagaagag
     1981 atcgacaaaa tgaaaaaaat gatcaaagaa atggaatgac cattatccgt ccaaataaaa
     2041 aaagaggatg ttccagaagt tatcagcttc aagaaataag aagaagctac ttctggaacg
     2101 ccgtgaataa ggggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     2161 aaatagcttc tcctattttt cgtttggtta ggcttattac cgcagatgca agcttttgaa
     2221 caccgtttat taagaggttg taaaatcagc gttctgacct gagtattaga gagacaaatg
     2281 gaaaaatagt tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga
     2341 acaccgttta tcgagaggtt gtgaaaaagt cgcttagttt cgcgtattaa gaaaaatttt
     2401 gtgaaatggc tttccacatt tttgtataaa tttggcttaa taccgagaaa ctggcttttg
     2461 aataccgttt attcgtaaaa gggattgtga caaaagtctt gtcacaatcc ctttttatgt
     2521 ttttattgtt ctaattgcgc tgtcttgctt actgcttctt gcggtttcac aaattttttt
     2581 gagattcccc aacctgtgaa ggcactgacc atcgaaaaca caggagaaag caaactgaaa
     2641 aatacgaatg gcaagtaagt gagtgtagga ataccaaatg tacttgctac gaaacttccg
     2701 gcaattcccc aaggcactaa gtagttgatt actgttccac catcttctag cgttctacct
     2761 aatacagttg gatcaaatcc tgctttttca taagtttctt taaatgcatt accaggcaga
     2821 ataacagata ggaattgttc accaataaaa atatttactc cgatacttgt catcaatgtt
     2881 gtaaaaatca aatttggtgt gttcgtcatc tttcttgata ctcgttccat cacggttgag
     2941 atcaagccaa attcgatcaa caatccacct aatgacaaag ttagaataat caatgacaca
     3001 gtaggcatca tactttcgat tcctccgcgg ctaagcagtg aatcgatttg ttggtttcca
     3061 gtatttgaaa caaaccctga ttcgatgacc ccagccaact tcgttaagga agtttgtggg
     3121 ttttgaataa aaatcattcc tactgctaca gcgatattca acaaaatcgt taaaattgcc
     3181 ggcacctttt tccatgcaca cagtaacatt aaaaataaag gaatcagtga ccagattgaa
     3241 atagtaaagt ttgcttctag aacatcagtc acttcacgaa tagatgaaag acttgcggct
     3301 ttacttgtgt gtcctaaaat catgaataaa aagaaagaaa caacaaatgc cgggacagtt
     3361 gaccacatca tatgcttgat gtgtttgaac aaatcggcat caacaatagc tgctgctaga
     3421 tttgttgatt cggataatgg agacatctta tctccaaaaa cggcacctga aacaatagct
     3481 ccaacaacca gcgctggatt taagcctagt gttgtcccaa taccgaaaaa tgcaatccca
     3541 atcgttgaca taacagtaaa ggcactccct acagcgctac ctacgattgc acacacaaca
     3601 aatacagaag ggacaaacca ctggacactg atcaatttga acccaatgac cattagagtt
     3661 ggaatgatcc ctgcttgtat ccatacagca atcaatgaac cgactaaaat aaaaataaag
     3721 attgggatga ttcctggttt gatgccattg atgattcctt cgtgcagcca atctgtcggg
     3781 tatcttttta tcactgcata caacatgacc attccgatcg ctgctaagat cgtgatatta
     3841 ggtgagacct ttaatccgac aacgccaatc gcaatgccta aaacaataac cattaataca
     3901 agtgttgctt ctaaaaaact tggtgttcct ttttttccca tgataatctc tcctttttta
     3961 gttgttctta tgttctaaaa aagttaagat atcggattac tccgccgcaa ttcatccgcg
     4021 attcctcctt tataaaaata aaaagacctg ttttaaacta aggacgaatg actccgcggt
     4081 accaccttaa ttaaaaacag atctgttttt cactttcaaa cttttattca aatgtatgga
     4141 ctttccatgt aattcgtcaa ttaggcttga tcggttcaca gcgaccaccg actttctgaa
     4201 caaaacacct aaccgctact gcgtgaaaag ttgacttgta cgaagtttac cttccataag
     4261 atcatttgtc aatgaaaaag aaaatatttt ttcatttttc tgaaaattat ttttataact
     4321 ttcacacaca agattatttt ttactagaaa gtagcgccct cttgtttttt tcttgctact
     4381 atttcttgat gttcttgctt gattctttct aatagctttg gctctttcaa cagatcaaga
     4441 gctgtattgg cgagtagttc tgctgcaatc gcaatagaag caaggccttt gtcactttta
     4501 gcagcagctt tgaattcttc tgtgtgtccg gcaatatatg tctcagagat tgctaccgta
     4561 ggctggattg ttggtatgac ttggctgaca tttcctacat cagaagaacc gagacttgtt
     4621 ctttgttccg tttctatttc ttcatctgga acacctgctt cttttgcatg ctggaagaat
     4681 aagtcatcaa aagaaggagt aacgatgaca tcatcaactg cattttggaa caatccaaac
     4741 tcaaaagtcg tgcccgtact tatcgcagca cctcgtacga tattttccac tttttcatat
     4801 actttatcta atgtttgccg atttcctgca cgtaaataga aacgtccagc agcgtaatct
     4861 ggaatgacgt tggcagctac tcctccgtct gtaatgatcc catggatatt gacatctttt
     4921 ggcaattgca agcgtagagc gttgattcca ttaaacactt ggatcaatgc ttccaaagca
     4981 tttactcctt tttcaggtgc tgctgctgca tgggaagcga caccgtaaaa tttgatgtcg
     5041 actggatcat tcgctaaaga ttctgtcgtt ttcccataac gaaaagctgg atggacgcat
     5101 aacgctacat cgacatcctc aaaaaaacct tctcgaacaa agctgccttt agctgatccg
     5161 ttctcgcccc cttcttcccc tggggtgcca tatacacgga tttctccacc agtttcatca
     5221 atgaattgct tcactatgct ggcggctaat acggagtaag taccaaacaa attatgcccg
     5281 catgcatgac caatacctgg taaagcatcg aattctgcca aaaaggcaat tgtgggaccg
     5341 ggtttattgg aaatataacg tgcatcaaag gctgttcggt gtccagctac ttctttttct
     5401 acccaaaacc tttctttttc taattgttta atcaacgctg ctgaggaata aaactcatag
     5461 ttacttactt caggatgttc gtggatatct agagcaagtg cttgatattc tggtaaacga
     5521 tattggatta gatccttcaa ttggtttgtt gagattgttg ttgtcataaa aaattcctcc
     5581 tatgctagtc atttttctta ttttctcccg aatgtttccc atgcaggcac gcttgatcct
     5641 tttgatgttt tctcgattac tttgactgtg tcttctgttt gataagcgtc tactattttt
     5701 tgatacgttt tattgttttc atcttctttt cttgcaacga tgatattcac ataaggacgt
     5761 gcatggtcat ccactggttc taagaagatg gcatcagaag caggattaaa tccagcatct
     5821 acagctacac cgctattgat cactgaaatg tctacatctg acaaagcacg tgctgtttgg
     5881 gaagcatcta attcagaaat gtctagattt tttctattct cggtaatatc agaaacagtg
     5941 ggcgcttgtc cttttgcctt atccactttg atcaatccag cagtttgcaa taacagaaga
     6001 gctctgccgc catttgtaac atcattaggg attgctactt ttgctccgtc tttgatatca
     6061 gctacatcct tcaaagtgtt agaataaatt cccagcggtg cattgacagt atttccgatc
     6121 gatactaaat cagttccgtg ttctttattg taattgtcta aaaagaattg atgttggaaa
     6181 gaattcaaat caatcgatcc atctgctaaa gccacatttg gttgattgta atctgtaaat
     6241 ttcacgtatt ccaaattaat gttctccttt ttcaaacggt ctttcacgtt gtcccataca
     6301 tctgtatctt ctccaataat ccctagtttt acgatagttt cttttttgtc agttgtatct
     6361 gcttttcctt ggctacaccc tgccaaccaa ataattgcta caaatgtaat gactacaaaa
     6421 cgaataattt ttttcatttt ttctcctcca ctttcaatgt gtaatttttt taatcagttg
     6481 attactgata aattgactta aaaaaactaa gattagaatc aataatgtag ccacaatcgt
     6541 tacatctgtt tggaatcgat tataccctct cgatatagca agattcccta atccgcctgc
     6601 acctactgct ccagccatgg tagtcagtcc gatcacgttg atgatagtca acgccgagac
     6661 ccgaatgata ccaggcaaac cttctaacaa atacacacgt aaaatgatac cgattggact
     6721 ggttcccatc gcttctgccg cttcgatcac cccaggatca acttctagca gtgcgttctc
     6781 gatttgacga gcaaaaaacg gaacaactcc aaaaatcaat ggcactaaag ctgctttcgc
     6841 accaatcgtc gttccaacta agaaacgagt agtcaatgct aaaagagaaa gcaagataat
     6901 gaaagggatg gaacgaatga tattgatcag cttatcaagt atttcaaata gcagacggtt
     6961 agctagtaat ccattagggc gtgtcactac taacgtcaca cctaagatga ttccaatcat
     7021 cccgccaatg aatgctgtcc aaaaagacat atatagtgtt tgcttcgtcg cttcgacaaa
     7081 ttcatccgga atcaaataaa cattgggcaa atatgtttta aagaaatcag tcattttatc
     7141 ttcctccttc gatcactttg atacgactat ctaaatcgtt tttttgtaaa atatttattt
     7201 ttacaccaga tttgatcaaa taatccaatg cttttttccg ctgtttggtt tctccagcta
     7261 aagtaacgat caaactccct aacggcgttt cttgtaataa ctctacattg ccatataaaa
     7321 tattcgctgt tacttgatat ttgctgtaca gatgagcgat tagaggttcg ttcgtctgat
     7381 ttccaatata acttaattcc accagccatt tattggcgat ctgatcggca aaagcagaat
     7441 gggcaatgat cgtttctaat gcttggtcaa gatgagtagc tgttcgaata aaatctttgg
     7501 tcaattcttc ttcaggattg ctgaagatct ggatgctgct acctttttca acgattcttc
     7561 catcttccat aaccgctacc ttgttgcaga tttctttgac aacttgcatc tcatgagtga
     7621 tcaggacgat tgttaaacct aactggcgat tcaatttttt taacaatgca aggatctgaa
     7681 gggtcgtttt cggatcaagt gcactggttg cttcatcaca taataatacc ttaggttcat
     7741 tcgccaaagc tcgtgcgatt gctactcgtt gtttttgtcc ccccgacagt tgtttaggat
     7801 aagcagatgc tttttcttcc aaaccgacaa gttccagtag ttcattcact ttttgacgac
     7861 gttcctgttt cgattttcca gaatacttta aagaaaaatc aacgttgtca aaaatagttc
     7921 ggctttccat tagattgaaa tgctgaaaga tcataccgat ggcctttctt tcttttcgca
     7981 attctttggg agagagctga ctcagattcg tctgattgac aatgacttct cctgctgttg
     8041 gtttctgcag caaattcatc acacgaacta acgtactttt ccctgcacct gagtaaccga
     8101 cgattccata aacatcgcct ttttcaatcg ttaaatctac agaatctact gcctgtatct
     8161 ctttttcttt ttgtttgaaa catacagaaa catctcgcaa ttcaatcatt aaaattcctc
     8221 cttatctaaa aaaagcgtcc tcaagaaaat gaaaatgtca ttttcttgag gacgcttttt
     8281 tgcgtgttac cacctcatgt tcaagctgtg ttcacacaca actcctcttc aagtacgatc
     8341 atactctagc aagatatcgg tcgcagccgt catcgcctta catctatgct cgggatgaat
     8401 tgctcgatgg ccattttcca atagtcgttc cttgcttctt ctcagctacc gaagctctct
     8461 gtcaaaggtt cttctatcgt acttaccatt tcaatgcaaa taatattcaa aaaaaaagtc
     8521 cttttactaa cataaactat gtcagtaaaa ggacgacgta tcgcggtacc accttttttc
     8581 ggaagtttcc ttccctcaag cccaacatct caaaaagaga gattggcgtt cgttttaacg
     8641 gtcgaacagt tccgaatttg cctacttatc gacaaattat tctccaaggc cattttcagc
     8701 ttttcttact tgactcttct caccagccag agcctctctg caaagtttcc aaacttactt
     8761 tccttttcaa cgaaatcgat attttgttgt tgggattaat ctacatgagg aaaactgaaa
     8821 agtcaagtac gaaattcaaa ttattttttc tatatttatt tttctgtttt tttatttaac
     8881 atttcaataa cagttcgaag atcttttcct tccgcaagaa gtttcaattt ttccttttct
     8941 tcgacggtat aatttcggac aaaactggaa tcaggtatca aaacaacgcc taatcctgct
     9001 cgtgaagcag ctgttgctcc tactaaagaa tcttccactg caaaggctga actagcttct
     9061 aatcctgttc gttccaaagc tgtcagataa ggaatcggtg aaggcttgtt ctccgacact
     9121 tcgtctccga atgtaaacgt gtccaagtaa tcagctaatt taaagtgatc caagatatct
     9181 gttgctcttt tcgtaacagt ggtcgtagca atgcctagtt tcatatgacg ttcttttaag
     9241 tctgccaata tttcttttgc atatggtttt aagtggatgg ctccgcttgt cagctgttga
     9301 taaaaatatt cttcacgctt cgctcggact tcctgtactt tttcagggct acctgctgct
     9361 ttcactaaaa tatcaaaact ttgtttccag ttttttccgc cccaagcgtc aacgatttct
     9421 tttgggatat ggatatcaaa ttgctgaaat ccccaaagcc agccatcacg atacgttttt
     9481 tctgtgtcaa ctaacaaacc atctaaatca aatattgcac ctttcatcta tacattaatc
     9541 ctttccaaaa caatacacat agtttagcac gactggtcca aaaagaacag aagcaaagaa
     9601 ccagccttct cgtttataag agttcttgga atcgacggat atagattttc ttcacagctg
     9661 tcacaagcgc cagataagcg atgactgtag caaataacca taaccagtaa ttgccaggaa
     9721 gtggcatcaa gcctaattca gcaccaaaac ttgtaaaagg aaggacagaa ccgacaataa
     9781 ttcctaatgt agtgataaca aacatggcaa atgttgcgtt gctttggata aatggtactt
     9841 tcggtgtacg cagtgcatgg agcactaagg tttgtgtcca tagtgattcg acaaaccatc
     9901 ctgcatggaa taatgcgatg aaaatcactc gctgtgaact atccaatgta tggaaacttc
     9961 cgcccactac agcaggacaa ataacaaaat acatcaacaa gtaagtagta atatcaaata
    10021 tcgaacttgt tggaccaaag taaaccatga attccccaat actagatgca ttccattttt
    10081 ttggctcgtc caaatattct ttatccatcc gatcccatgg caaggaaatg caggaaacat
    10141 catagatcag attcaaaaac aaaagttgta aaggcagcat tggtaaaaat ggcaggaacg
    10201 tacttgcaat cagcacggaa aacatattgc caaaattaga acttgctgta gccttgatat
    10261 atttcatgat attcccaaaa gtttctcgtc cagaaagaag gcctctttcc aaaatcgtca
    10321 aatctttttc taaaaggatc acgtctgcag attctttcgc aatatccaca gctgtatcta
    10381 cagaaatacc gatatcagcc actttcattg caggagcgtc attgatacca tctcctaaaa
    10441 atccgacagt atgaccattt ttacgtaaag ctgtcgtcaa acgtgctttt tgctgaggat
    10501 tcaattttac gaatacttca tggtcttcag ctacttgtgc taattcatga tcactcattt
    10561 ccgagatttt ctcaccggta accaattcat tgatctctag acctacttga cggcacactg
    10621 aacgtgtaac taattcgttg tccccagtca gaacttttac agctacacca tgttctttca
    10681 gagctttcaa cgcttgtttc gttgtttctt ttggtggatc aaggaaagca agataaccaa
    10741 tcaaaaccat gtctgactca tctttgactg aaaattctcc aacgacgctt ggattggtct
    10801 tttgtgcgac accaatcact cgtaagccat cttcattaag tgactgtact tttgcaagta
    10861 cggcttcttt tcgttctttt gttaatggtg taacagtacc gccaagatca ataaatttag
    10921 aaatgttcag catttcttca atcgcacctt ttgtgatcat ttgtgtttta ccagctggat
    10981 cagcaaccac aacactcata cgacgacgtt caaaatcaaa aggaatttca tccacttttt
    11041 gataattgat cttatctgta tctaaagttt ctgttgcttc atcaatgata gccacatcta
    11101 acaaattttt cagccctgtt tgatagtagc tatttagata cgcatggcgc aaaacacgat
    11161 catcttcatt tccttcacaa tccatatgat attcaagaat gattttatct tgtgtcaatg
    11221 tccccgtttt atccgtacat aacacatcga ttgcaccaaa gttctggatg gcattcaaat
    11281 ttttgatcac tgttcctttt ttggccatca cacttgctcc tttgaccaaa ttcgtcgtaa
    11341 cgatcattgg aagcatttca ggtgttagac caactgctac agatagtcca aaaagaaaag
    11401 cttctagcca atcccctttt gtaagcccat tgatgacgat aactgctgga gccatcaatg
    11461 acataaattt gatcagtagc atggatgttt tatggatgcc taattcaaag cttgattgaa
    11521 ttggtttttc agatagcgtt tctgctacat gaccaaatag tgtttcattt ccaacagaaa
    11581 caatgattcc ttccgcactt ccactgatga cattacttcc cataaagaca agattttcgt
    11641 aacttgtttc gctttgctga tcgtcttcac atactttcgc atgcttttct actggatagc
    11701 tttcccctgt caatgctgct tgggaaatga ataaatcctt tgcggacaat aaacgcatat
    11761 ccgctggtat catatcccct gctgaaagcc gaacaagatc tccacagacg atttcttcca
    11821 taggaatctc tgtgtacttt ccattacgtt ttacagctgc cgttactttc acaagtgatt
    11881 tcagtttttc agccgcttga ttggaacgta cggactgaat caacgtcatt gtcccgctga
    11941 cgataaccat agtgaagata atggctacac cgaaaagatc tttatctcca ggagctgcta
    12001 atacatattc cgtgatgaat gaaataattc ctaatgcgat caacactaat gtaaatggcg
    12061 tgatgtatgc tttcaatatt tctacaatga atggtgtttt tttgccatat gaaatcgcat
    12121 tttcaccgta attttctctt gaacgttcga tttgttcttc tgtcaatcca tttgctgatg
    12181 catggaatag ttcaaataat tcttgtttcg tttttccagc aaaagctgca tagatttttt
    12241 gtgtatcttt ctttttttcc atctcaatct ctcctttgtg tccaaaagac agctgttctc
    12301 gctgcttttg gtctttagct attcatcgga tcgatagaga aaaaactgaa aggggctgag
    12361 aacagataga taatagccct ggcctttcct ctagatcctt gtttttcgaa tgactgtcat
    12421 ccatttgttc tcatctcctt tactaagtaa ttttaattca tgacttccaa tcatttttcg
    12481 aatagttatt tttccaatta gaccacctcc tttttatttt tcaccgatgg ggatttgcgg
    12541 caacaaaaaa acctctcgcc aaatactcag cgaaaggttt gaaaaagcat attaaaaaaa
    12601 gcttctcaga tctcatcgtt gagttttgac actgtacaac gtaaaaagca gatacctgct
    12661 tttagctacc ttatgaaaag ccttatctcg acaattcctg ttctacccat tggcgtctct
    12721 cgacgttttt gggcagtagc ctatgtttgt ataggagcct cacctaacaa atcttattcg
    12781 gttttccttc gttaatataa caactaaaaa agaggatgtc aaatcaattt tccatgtttt
    12841 tcatactttt tttaaaatac acacgttata ttcctgtctc acgcggtttt atttgtctca
    12901 ttttccacct cccgctataa tagaaatata gtaggaaacg aggtgctgtt atggaaagaa
    12961 cacttgttat tatcaagcca gacggcgtga gacgccattt agtcggaagc atcatccaac
    13021 gttttgaagc aaagggactg gccattgctg aaatgaagtt tgaaactatg actcctgaat
    13081 tagcaaaaga acattatcag cacttgactg agcgcagttt ttttgatgaa ctgatcgatt
    13141 acatgacttc cggtcctgtc gtttatcttg tgctggtcgg cgaggaagtg atcgatatcg
    13201 tccgtaagat ggttggagca acaaaagctg ccgatgctgt tccaggtacg atccgaggag
    13261 actacgcgct gccggggact gaaaacatca ttcacgcttc tgattcccgt gatgctgcgg
    13321 taaaagagat cgcccgcttt ttcccgccat tcgatataaa caaaccgtct gaaaaagcgg
    13381 cgaagtgata caaattctaa actgctggtc tagttgctgg gttcagcaat ggccagcagt
    13441 tttttctcta aggacctccg ttgattcttt cctaacatat attcgagcac tgtgaagaat
    13501 tcttggtagt ctttcgtctt ttcttcccct aaagcagcag aaatcaatct ttgataataa
    13561 tctgccatca atgtttcata gatcactgta cttctttctt tatgtgctaa gctccgtttc
    13621 atatcttcat tcacactacg tgcctttttc aaccgaccat tttctatata aaacgaagtc
    13681 aggttcatta aaaaagtaaa gagatactgc tcatgattgt tcaactgtgc taatcgtcgc
    13741 attctttttt tgattttcaa ataattgttc tcaatgaacc gatcactgaa tatccaagga
    13801 aaatcagcta gaatagatag ttcaaattga ccatagtctg gtatctgcat caagtattct
    13861 tcaatgattc ggcattcata acgtaaaaat tcaaattctg tctcagatac ttctgttttt
    13921 tcgaccatgt gaagattcaa aaccaaataa tacagatctt ctgttttctt aaaatgccta
    13981 cggttattgc tgatctcttt ataaggatca aagctgtctt cttgtacccg atctagcatt
    14041 cgtctttttt tctttaactt tattggttca tccagctggt ttcgcatcca ttcatattct
    14101 gcaagtgtca tgttcattcg ctctaaaaat gatctcaatg ttacaaatga catttttttc
    14161 actgaatttg tctcaatccc gactagagaa gtcttaaaac agattccttc cgctaattgg
    14221 gactgtgtca acttcttgtt gactcgaagt ttcttgatca attcaccatc atacataata
    14281 gttctccttt ttaatttaaa tttttccaca ttcaataaat agatacgtta aaaaggaaaa
    14341 atacacgaaa aaaataaaaa aatgtttttt tcataaaaaa taccaacctt caagcattag
    14401 aatgttgtct aactactgaa ggttggtttg attgtttatt gattattttt ttgctgttca
    14461 acgtctgttc ttgttactgg atataagttg gttgttgttt tattaccatt ttttgaaaag
    14521 acacttgtcg aatgttcgcc tttttgcttt tcgattgctt cattacgttc cgcttcattt
    14581 ttatagtcat attttttcgg atcaatcgtc tcaaatccgt ttggcacata aaaccgcaag
    14641 agattttcct gattcagctt atctgaaaga cttaacgcag tcttgacttt ttcctgatct
    14701 ttggagattt cctcatcgat tcctgctgct ttcgtatcga tgatttctcc tgtctgatta
    14761 ttgtaagctt ttcctcctag gacggtgtat ttgggtgtca caaagttccc atttcgaaaa
    14821 gcaactactt gatcatgctg agaagataat aagtctgtac caaaatgaag ataatcttta
    14881 tcatcgattc ccaatagatg tagtaatgtc ggcaaaacat cgatttctcc accgtattca
    14941 tgattgactg tccctttagt ataaccaggc atatggatca tcaatggcac tcgttgcatc
    15001 tgtgcgttgt caaattcatc ccatgtatct ggatctttac caagagcgct tgctaaatct
    15061 ttattatctg tattcgagat cccaaaatga tccccgtaaa tcacaaagat tgaattttgg
    15121 taaataccag attttttcaa atacgtaaaa aattgttcca atgattgatc cagataatgt
    15181 gccgttcgaa cataattatc tactgtgcta ttgccagtat tcaaagatgg aaagtcgaaa
    15241 ttcttgtcat ccgtataata aggcgtgtgg ttggttacgg aaagaaattt cgtgtaaaac
    15301 ggctgttgta gatgctcaag atattgggca gattctctga ataaatcttt atctaatatc
    15361 ccataaccta acgtttcgtc ggattcatca aaataagatc gatcaaagaa attgtcataa
    15421 cctaagtttt tatatacatg atctctattc caaaaactag ctacattccc atggaaaaca
    15481 gcgcttgtat agccttgctt ttgtccaagg atcgctggtg cggcttgaaa gacattgtct
    15541 gacccaagtt ctgtgaataa tgagccttga ggtaaaccaa atgtgccagt ttccaacata
    15601 ttttcagcat cacttgtctt tccctgtcct acttcatgaa agaaattgtc aaagctgatc
    15661 gttgcttgat tctggaaaat actatttaaa aacggtgtga cttcttgtcc gtctaccttc
    15721 atattaatca aaaactgctg gaaactttct aaatggagaa cgatcacatt tttcccttta
    15781 gcgatcccaa atgtttcggg atttggctct gcataatgtt tctttgtata atctaaaact
    15841 tcatcaatcc cattactgct tgcatgcgcg cgaacactgc tagtcattcc tgttttgatt
    15901 ccgtcataaa tagtatacgc atcgattcct agatatttga caatatatga acggtcaaat
    15961 gttcgctgca gcaattgtgg acggttggct tcgctcagcg ctagatttcc tgaaaaaata
    16021 agccctgaaa gacaggtaac agcgatagcc atcggtttgc cgacagggtt gcttttgata
    16081 ggtattttct tgaccttcaa ccaaaccagt agcccaatga acacagcgat atccagccag
    16141 tagatgacat catggggctt catcaaagaa aaggagcttc ctgacaatcc ttgcgacact
    16201 ttcgaaaagc ctaatacaga tttgactgta atgaaatctg tgaattcccg ataaaaaata
    16261 atattcaagt acaacaaaag cgtatttaaa atatcaatga tcaaaagaat agctaacgct
    16321 ggctttggct ttttaatata caaagaaagg ccaaaaaaca ataaggtagt cgcaattggg
    16381 ttgatccaca agataaagta ctggatcgta ccttccacac ctaatgaaaa atctgcatag
    16441 taggcaatga tcgtcttcag ccagaaaaag aaaacagctg tcaaaacgat tgctaaacga
    16501 ttttggcaca attttttaag ttgtgtcacg tatatcactt ctctctattc acattttttc
    16561 ttattttgct cctgaaaaat gaaaatttcc taaaaaagtt gttatcttaa gattttttta
    16621 ttctttctaa agattattcc gtccactttt cacagcataa aaccgctgcg atgtaagcga
    16681 ttaaactaat gaagagataa aagtcttgtg gtgaccgctt cactccacgg tattcaaaag
    16741 ccagtttctc ggtattcgta caaaaaaaga ggctgtgaca aaattcttgt cgcagctcct
    16801 tatttcttga tgcaggacag ctttttcaca acttcttcct taaacggtgt tctcctgcca
    16861 actcctcggc cacaagtcac aatatttata aaacatgaga agctgttttt tgaaattgtt
    16921 ccttgtggct cagagttaaa cggcaggctc aagacctctt gatacacggt gttcaatcag
    16981 ctgaccctcg gtattagatt atgtcaggac gctaatttta caacctcttg gtaaacggtg
    17041 ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg aaaaatataa ggagctattt
    17101 tttgaaattg ctccttgtgg ctcagagtta aacggcaggc tcaagacctc ttgatacacg
    17161 gtgttcaatc agctgacctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
    17221 ttgccgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct ctgtttttca
    17281 ttgtccatag gattgatatt gtccttgtga tgactcattc gtaataattg aggcttgatt
    17341 ttcttcactt cccgttatcg tttgtccact gccagaatct gaaggacttg ttcctgtcga
    17401 ctcgtttttt tgagaagaga cagttgtttt cttgacttgt tcatttgtct ttttccattc
    17461 cgctaaacga tcggcgacca cttttttgtc tactttttca gaataagcgg tgatcacttt
    17521 tccatttttc aaacgggcta ctttgtcgcc atcatgagtc aaaagcaaaa cggtcaaatg
    17581 cttgtcttta aattgatatt gtctgagaga aatcgcacta agtacgtcat cgatattaat
    17641 aaattgattt tcttcttttc cttttggcaa ataaatgtcc aattcgcctt tttcgtaatt
    17701 ggcagttatt ttatactccg cccaccaata agttgagaat tcttttttga aattatctac
    17761 tgattctttg cttgctacga ttttttctgt ttctttctga gcaagatctg ctgtcatttt
    17821 tcctgaaaat gctgcggata aaataagcag taagaaaaaa ccgaataacc aaaagagaag
    17881 aaacggtctt tttttcctca tgctttcctc cactcctat
//
LOCUS       DAJSKS010000051        17665 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJSKS010000051 DAJSKS010000000
VERSION     DAJSKS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.052"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            56..1741
                     /locus_tag="ON396_001435"
     CDS             56..1741
                     /locus_tag="ON396_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCR3113526.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            1755..3038
                     /locus_tag="ON396_001436"
     CDS             1755..3038
                     /locus_tag="ON396_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR3113527.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            3108..3977
                     /locus_tag="ON396_001437"
     CDS             3108..3977
                     /locus_tag="ON396_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3113528.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            3974..4810
                     /locus_tag="ON396_001438"
     CDS             3974..4810
                     /locus_tag="ON396_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3113529.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            4836..5903
                     /locus_tag="ON396_001439"
     CDS             4836..5903
                     /locus_tag="ON396_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR3113530.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            5900..6727
                     /locus_tag="ON396_001440"
     CDS             5900..6727
                     /locus_tag="ON396_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR3113531.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            6772..7797
                     /locus_tag="ON396_001441"
     CDS             6772..7797
                     /locus_tag="ON396_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3113532.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            7794..9977
                     /locus_tag="ON396_001442"
     CDS             7794..9977
                     /locus_tag="ON396_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCR3113533.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            9977..11107
                     /locus_tag="ON396_001443"
     CDS             9977..11107
                     /locus_tag="ON396_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR3113534.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            11104..11781
                     /gene="pgmB"
                     /locus_tag="ON396_001444"
     CDS             11104..11781
                     /gene="pgmB"
                     /locus_tag="ON396_001444"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR3113535.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            11865..12392
                     /gene="msrA"
                     /locus_tag="ON396_001445"
     CDS             11865..12392
                     /gene="msrA"
                     /locus_tag="ON396_001445"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR3113536.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(12593..13279)
                     /locus_tag="ON396_001446"
     CDS             complement(12593..13279)
                     /locus_tag="ON396_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR3113537.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            13359..14897
                     /locus_tag="ON396_001447"
     CDS             13359..14897
                     /locus_tag="ON396_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR3113538.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            15017..15484
                     /locus_tag="ON396_001448"
     CDS             15017..15484
                     /locus_tag="ON396_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCR3113539.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            16003..16446
                     /gene="rplM"
                     /locus_tag="ON396_001449"
     CDS             16003..16446
                     /gene="rplM"
                     /locus_tag="ON396_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR3113540.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            16462..16854
                     /gene="rpsI"
                     /locus_tag="ON396_001450"
     CDS             16462..16854
                     /gene="rpsI"
                     /locus_tag="ON396_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR3113541.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(16942..>17665)
                     /locus_tag="ON396_001451"
     CDS             complement(16942..>17665)
                     /locus_tag="ON396_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3113542.1"
                     /translation="RTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDD
                     FNNHLLTFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTK
                     GRKDTVIQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHAD
                     YLNYRINSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYV
                     NTPDIVDKAVFEAFQRGLNKCD"
BASE COUNT         5741 a         3241 c         3800 g         4883 t
ORIGIN      
        1 ttccataata tgatcaaatc gttgggaacg ctcccaaaaa agaaagggga atccgatgta
       61 tacaaaactt ttacaagcga tttatcgcga ctcagatttg tctgagaaaa ccgcctctat
      121 gattgaacaa aaagtcagtg tttatcagca gaagaaaggc caggcaggca aactgcattg
      181 ggacgaagaa gatattttct tgattactta tggggatcaa ttttttgaag agcagcaacc
      241 aactttgcgt acatttaaga aattttatca aaagtatttg gcagatacat ttagagttgt
      301 acatttcttg ccatttttcc catatacatc tgatgatggt ttctccgttg taaattatca
      361 ggcaatcaac gaccgtttgg gcacatggga agatatagaa gaaatgagaa gcgaaacacg
      421 gttaatgttc gactttgtct gcaatcatat gtctgccgaa agtacttggt ttcaggaata
      481 tttaaacttg aatgatacgt acaaagattt ttttattgat ttgccactgg atacagatac
      541 aagcagcgtg actagaccaa ggaccagccc gttacttaca acgttcattg acaaaaacgg
      601 gaaagaaaaa cacgtatgga cgacttttag cgaagatcaa gtagacttga attttaaaaa
      661 tccagaagtt ttattgaaaa tgatcgatat tcttttgttt tacttagaaa aaggcgcttc
      721 atttattcgc ttggacgctg tgggattttt atggaaagaa ctaggaactt cttgtataca
      781 cctgccagaa acgcataaaa tcatccaatt attccgtgca atcgctgatg aagcagctcc
      841 aggaacggta ttgatcactg agacaaatgt gccacacaaa gataatatca gttattttgg
      901 gaatggcgag aatgaagcac agatggtcta tcagttttcg ctaccacctt tgacccttca
      961 agcgattcgt tcgggtaata caagtaagtt gaaagaatgg ttaaaaacca tcgatttcgc
     1021 atcgaaaaaa acaacttttt ttaatttttt agcctcccat gacggtatcg gattgaatcc
     1081 ggtacgaggt attctgccgg aaaaaacgat ccttgaatta gtggaacagc tgaagaaaga
     1141 gggtgcccga gtaaactaca agaaaaaccc agatggaacg accagtccat atgagataaa
     1201 cgctacgtat ctagatgcgt taagttctcc aacagatacg acggaatgga gaagcaaacg
     1261 ctttttagct gctcattcat ttttggtaac gttaccaggc gtgccggcaa tttatattca
     1321 aagtgtgctg ggaagtcgaa atgattacaa aggcgtagaa aaaacaggag agaatcgaaa
     1381 aatcaatcgg caaaaatttt tgttagcaga tattgagaaa gagttgcaga cagatccatt
     1441 aagaacagca gtatatggtg aattgaccca gatgctatct attcgaaaaa aagaaaaagc
     1501 cttccatccg aatagtttga tttacttact ggactcagca gattctttta tcagttttat
     1561 tcgcgaagcg aaggaagaga aagtcttagt tatccataat ttttccaatc aatcagcagg
     1621 ttatctttta ccaaaaggag attacctaga cctgttttct ggacaaatta tccattgcag
     1681 tcaagaacga aagttgatat tccagccgta tgaatatatg tggctgaaaa taataaaata
     1741 agaggtggaa aaaaatgaaa aaaattattg ttactacctg tctgctagct actggatttc
     1801 ttcttactgc ttgtggtgaa agtcaaagct ctaacgaatc acaacagaca acgattcaat
     1861 tcatgcactc ttctgtcgaa caggaacgat taagtgtgat caatgaactg gttgctgatt
     1921 ttgaaaaaga aaatccagac atcaaaatca agcaagtgcc ggtagaagaa tcagcgttca
     1981 atacaaaaat cgttacgtta gctcgttccg gtaaattacc agaagtaatg gaagtcagtc
     2041 aggattttgc taaagtgatg gataaagatg aactgattga tcagacggca gtccaaaacg
     2101 ttatcgagga agctggagaa gataattatt atgacggagc caaaaacctt gttcgttcag
     2161 aagatggaaa aagctatatt gctgcaccaa ttagcggctg ggttcaaggt atttggtatg
     2221 acaaagaaaa attatcagaa gctggttttt cagaaccaga aaattgggat gatattttaa
     2281 aaattgctca acatttcaca gataaagaga ataaacaata tggaattgct atgcctactg
     2341 ctgaaggaac aatgagtgag caagcgttct ctcaatttgc tttgtcaaat ggagcaaatg
     2401 ttttggatga tgaaggcgag gtaacgatcg atacggaaaa gatgagagaa gcattgtcgt
     2461 tttatcaaaa cttatcccaa tacacgatgc caggctctaa tgatgtgaca gaaatcaaag
     2521 atgctttcat gaatggaaca gcaccaatgg caatctattc aacttacatt ttgccttctg
     2581 tttatgaagc ggggaactca gaaaatatcg gttttgctat cccaacacaa aaagatcaag
     2641 ctgtctatgg cacagtatct ggattgacga tttcttctgg tctagacgag aaacaaaaag
     2701 aggctgctga agcattcaca gctttcttat ctcaaccgaa aaacatggaa aagtgggtct
     2761 tgatgtctcc tggtggtgct caaccagtga ataaacaagt agtcgaactg gatggttaca
     2821 aagaaaatga agtcatcaag tcttttggcg agctgccatc tgagattgct agtgcttttg
     2881 atgaagtaca agtctttgga ctagtcgggg aaaagaattt cacgaaaatg ggagatatca
     2941 caagctcagg tgtgatcgga aaagcagtga accaagtaac tgtcggaaac gaagatgtcg
     3001 atcaagcttt agctgacgca caaaaaaata cgaaataaca agaagttctg ccaatggtgt
     3061 tcgaaaaaag tagatagcta ttggcagatt atcaggggga ggtttcaatg aatcgtccaa
     3121 ggaaacattc tgacttaaaa ttagccatgg gacttttagc accaagcatg cttttattag
     3181 ctgttttagt gctgattcca atgctttcca atattcagat cagttttttc gatatgccgc
     3241 tgaatcctaa ctcagacgcc gtttttatcg gattaagcaa ttatcttgaa attttaagtg
     3301 atagtgaatt ttatcgttct ttaggtttaa cagttgctta cacattctta gttgtagcag
     3361 gcagtacagc tttaggatta gccgtagcta ttttgcttaa ccgcaagttc agatttagga
     3421 aaacggtgcg ttcactgatt ttattaagtt atgttacgcc atctatttct ttgatttttg
     3481 cttggaaata tatgttcaat aatagttatg gaatcgtcaa tgatctgtta gtagataggt
     3541 tgcagctttt caacgaagca ccgctgtggt tcgaccagcc agttgctagc ttctttatgg
     3601 tcgttttgtt tgctatctgg agatactatc cgtatgcgtt tatttcattt ttggctattt
     3661 tgcaaacagt cgacaaaacg ctttatgaag cagctgaaat ggacggtgca aatgcttggc
     3721 agaagttcaa gatcgtgaca ctgccggcaa tcaaaccagt actagcaact gtgattgttt
     3781 tacgatctat ctggatgttc tatatgttta cagatgtcta tctactgacg aataaagtca
     3841 atatcttagg cgtttatttg tatcagacag cctttgcgtt caatgatctt ggaaaagcag
     3901 cagctatatc gattctattg ttcatcatta ttgcagcggt cgtcttgctc tcacgaaaga
     3961 aggtggactt gaaatgaaaa ataaaaagag caggaaaatc ttattttacg cgacgatttt
     4021 gttgctatta gctgtcaatt tgtttccatt tttgatcatg ctgatgacat cattcaagga
     4081 ttcacaagaa gcaattgcaa cgacacaaac tatttttccg aaagaatgga cttttgaaca
     4141 ttatcaggat attttcaatg ctgagatttt tccattcatc atgtatttca aaaatagttt
     4201 agtgatctcc ttgatagcct ccgtaactgc agtagtgatt gggatcagcg gggcatacgc
     4261 tttgtcaaaa ctacgcttca aagctcgctc gaaaatcaat gctagctttt acaccgttta
     4321 tatgttttcc ggtattttga tcgttgttcc attatttaaa atcatttccg gtctaggttt
     4381 gtatgatacc aatacagcac tgattatcac gatggtcgtt caaacacttc ctactgcgat
     4441 attcatgcta aaaagttatt ttgacacgat tccgactgat attgaagaag cagcaatgat
     4501 cgatggtctt agccggatcc agatcattat tcggatcatt ataccattgg ctatttctgg
     4561 aatcatttct gtcttcgttt attgctttat ggtttcgtgg aatgattatt tatttgcttc
     4621 gattttcctg tcgacctccg aaaaatttac tttaccaatc ggtttgaaca cattattcag
     4681 tacaccagac tacatttggg gacgaatgat ggccgcttca ttggtgaccg ctttaccagt
     4741 catcattatg tatgcgttat ctgaacgatt tattaaaggt gatttagtcg aaggcggcgt
     4801 aaaaggataa aaagaaaaga aagaggaaag aaaagatgaa aaaattagtt gcattagaac
     4861 caagaatagc cggattcaaa gattacgaag accgtgagat cgaaaagaac gaagtacgta
     4921 tccgagtaat tttcgctact ccaaaaccag gaacagaaat tgtcgacttt agaggattaa
     4981 gtccatttat cgatgaagaa ttttcagaag aatggcgcct attcttgcca agagaagaag
     5041 gaagccaacg cggtgtggta tttggcgaat ttcaattagg aaatatgatt gtcggtacga
     5101 tcgaagaaac gggcagtttg gtgacagagt atcaaaaagg agagttagtc tgcacttatg
     5161 gacccattcg tgaaacgctg attacaaacg gtgttgataa ttataaacta cgtaaacttg
     5221 atcggttgga tcaatggaaa aatgcagtat gttacgatcc tgctcaattc gctttaggtg
     5281 gtatccggga agcacatgtt cgagcaggtg atgctgtagc aatcttcggt ttaggcgcta
     5341 tcgggcaaat tgcgattcaa ttagctaaaa aagcaggtgc tactaccatc gttgggatcg
     5401 atcccatagc gcagcgtaga gaattagcag aaaaatacgg tgccgattac acgctagatc
     5461 ctatgaaagc agatgtcggt tttcgtctaa aggaattgac agataaacgt ggagtagatt
     5521 cagttattga aacgagtggc agtgctgccg cattacaagc ttctttaaga gggatcggct
     5581 atggcgggat catttcttat gttgcctttg ctaaaccttt tccagctggg tttaatcttg
     5641 gtcgcgaagc acatttcaat aatgcaaaaa tcgtctttgc tcgagcatct agtgaaccaa
     5701 acccagatta cccaagatgg aaccgtaaac gaatcgagga cacatgttgg gaattattga
     5761 tgaacggtta cttggattgt gaagaaatca ttgatccagt tgtgccattt gcagaaagcg
     5821 cagaagctta catgcgttat gtagaccagc acccagaaga aagtatcaaa atgggcgttc
     5881 aagtccgagg aggagaacaa tgaaattcgg tacgcaagat caagcatttt tcccaagaga
     5941 cattttagaa aaatttagat ttatcaaaga ctgtggattt gaagaatacg aaatcgacgg
     6001 taagttgtta atagaaaata tcgacacagt caaacaggca atcaaagaaa cagggatccc
     6061 agtagtgaca gcttgtaacg gctacgatgg gtggatcggt gactttatcg aagaacggcg
     6121 tcttaatggt gtacgtcaaa ttgctgagat tttgcgtgca ctaagcgagg ttggaggcaa
     6181 aggaatcgtt atcccagcag cttggggaat gttcacttat cgattaccgc cgatggtttc
     6241 accccgaagc aaagaaggag actataaagc agtcagcgaa tcacttcgtt atttagataa
     6301 agtagccgaa gaaacaggta cggttattta tttggagcct ttgaaccgat accaagacca
     6361 tatgatcaat ttgttagcgg atgcgcgtca ttacattgac tctaatcaat tgaaacatgt
     6421 gcagatcatc gctgattttt atcatatgaa tattgaagaa gatgatattt caaaagcatt
     6481 gactacgcaa aaagagtatg tgggtcatat ccatttagca gataatcatc gctaccaacc
     6541 aggatctggc gcaattgatt ttactcgcca ttttcaaacg ctacttcaaa atgagtatga
     6601 aggttatatg gtatatgaat gtcgtttgcg aggagaaaat gaagtgaaaa tttatcagga
     6661 atcattgcaa tacttgaaaa actgtctgga aacagcgcgt catttaaatg atttagagaa
     6721 agaatagaga aagaataaca ataagtaaat aagcaaagga gggagaaata aatgaagaaa
     6781 gtagcaatca taggcgctgg acaagtagca gaaaaagtac atgctgctta ttatcaaaaa
     6841 agatcagatg tcttagaatt ggccgctgtg gttgatccag tcagccagcg agcagaagac
     6901 ttttgccgac gtacaggatt ttctcatgcg tatacatcag ttacacaaat gctgaatgag
     6961 atttctcccg atatcgtgag tgtctgtaca cccaatcgct ttcacttcga acatgtattg
     7021 gctgccctcg aagctaatgc ttcagtgatg tgcgagaaac caccagctat gacagctgat
     7081 caagcaagaa agatggcaga gacagccgat aaaaacgggc agattttggc ttatggattc
     7141 cagcatcgtt tttctgacga agcaacaatg atcaagaatc atcttgatgt tctcggggat
     7201 atttattacg tagaagcgaa tgcgcttcgt cggtctggtg ttccaggctg gggaacgttt
     7261 atcgacaaag aacttcaagg aggcgggcct ttgatcgatt atggcattca catgcttgat
     7321 acagcgatgt atattactgg atttcctaaa gtaaaaagcg ttcgggcgta cagcttccaa
     7381 aaaattggta cgaaaaaaaa ttcagggaaa ttcgggaaat gggatccaag caaattcaca
     7441 gtagaagatg caatttttgg cacactggaa ttagaaaacg gcaagatcat ccaactgaat
     7501 acttcgtttg ctttgaatat cagagaagac aaactgaatg tggctttttg tggcgatcag
     7561 gcaggtgcta atctgtatcc aggcgaacta ttcgaagatg tagatggaaa gctggtttcc
     7621 ttacaaaaga ccacgccagt taatgaccca catgaactaa gtatggctca ttttgtgaat
     7681 cgggcgctcg gtgaccagac tcagatgatt gcagatggct ggcaaggata tgtcatccag
     7741 aatctggtgg aaaatttata tcgttcggca gaaacaggag aagttattac cttatgaaag
     7801 tagaagaaaa acagtttgat ttagagtata tcaataagta tgcgagtcag atgaccattg
     7861 gaaatggatt ttttggtatc agaggaagcc aggaggaaga ctatcggaat caagtaaggg
     7921 gaatgtttgc agctggtgta tataaccgac ctttaggagc agaatcagcc gagttagtga
     7981 atctcccaga tgtcatcaga tgggaaataa aaatcgacgg tgaacatttt tctcttgaga
     8041 cgatgaaagt tcttgcatac gagcgggcac tagatttaga aatgggagag cttaaacgct
     8101 atgcgctcat tgaaactgct caaggaaaaa aagtggaagt cattacttgt cgtgttgctg
     8161 atcaaaataa tctccatcag gcagccctga aagtggtcat caagccgttg accgaagact
     8221 gccaagtaga tgtgaaaacg ggtatcgatg gacagcaaac caattttggt acacagcagt
     8281 tggttgaaaa agaactgcga gtatttggtg acgatttgat cagtgcatcc taccagacaa
     8341 cagaaagtaa aattgatatt gggattgcag ctaaattcaa taaagcaggt gtattccaag
     8401 caaaaaatcg ccggatcacc gcttcctttt cagaaaagat cagaaaaaat acgtccttta
     8461 cgctggagaa gatcatcgtg ctctctacct ctcttcagca aacagacccg tttaagaaaa
     8521 gtattgccca agcccagaaa agcgttagtt atacagaaat tttagagcag tcgatgtctt
     8581 attggacaga tttttggcat cagcatcgga taacgatcca aggcgattct tctttcgatc
     8641 agttagcgat ggacttcgct gcttaccatt tacagattat gactccccgc aatgatccaa
     8701 gtgttagtgt aggggcgaag ggactaactg gcgaaggtta taaagggcat gttttctggg
     8761 atacggaaat ttttatcctg cctttctttt tatatcagtt tcctgaagta gcaaagaatt
     8821 tgctgctgta ccgttatgat cgattagaag gcgcacggca aaaagcaaga gattatcact
     8881 atcaaggtgc acttttccca tgggaaagcg cacacactgg tcaggaagag acacccaaat
     8941 ttgctgcatt gaatatcaaa accggaacaa gacaagaagt tgcttcagct gccgcagaac
     9001 atcatatcag tgcagacctt gcttatgccg ttcaagagta tgtccaagca acagaggatc
     9061 aatcgttttt agaaacaaag ggaaaaacgc tcattcatga aacagcagct ttttggctga
     9121 gcagagctgt aaaagtcgat gatcatttag agatacacag agtcattggt ccagatgaat
     9181 acacagaata tatcgacaat aatgcatata cgaactatct ggcgcattat aatgtggcct
     9241 tagctgttaa atatcaagct ggcgatccgc tttttttgaa agaatgtcaa aattttttag
     9301 ataagttata tttgccagtg cctaacaaag aacagctgat tccgcaagat gacacgtatc
     9361 tcagtaagcc agttattgat ttgaccactt acaaagaacg tcctggcagc cagtctattt
     9421 tacttgatta tgcccgtagc gaagtaaatg aactccaagt cagcaagcaa gcagatcttg
     9481 tcatgctgtt atacttatta cctgagtatt tttctcaacc aatcgtttct aagaatttgc
     9541 attactatga aaacaaaaca atccacgact catcgttgag taaagcgatt cacgccatag
     9601 aggcttgccg tatccaagaa atggaatggg cgtatcattt attccaagaa gcgtgcctga
     9661 ttgatttagg accagatccg cattcatcag atgacggaat tcatgctgct tcattaggag
     9721 cactatggct agcagttata tttggttttt caggtattac agtaacgaat ggaactcttt
     9781 ctatttctcc aagacttcct tcagcatggg aaaaaatttc ttttcctttt atttggaaaa
     9841 atcacaggtt gctatttgtt ttgacaaaaa aacaaatagt aataaaaaaa gagacgaaag
     9901 aaacattacc tatttttgtc aatggagacc aataccagct ggaaaaagaa ctgatcttgg
     9961 aacggagggg aaaataatgc gtattctgat tgcgccagat tctttcaaag aaagtctgac
    10021 agccttagag gcagcacaag ccattcaatc aggttttcaa gaagtttttg ttcaggctga
    10081 atatgatctg ctacctatag gcgatggagg agaaggaacg gtttcatcat tagcagacag
    10141 tttaaggttg aagcggcaaa cagcagtcgt tactaatgca tttggcaaaa acaaagaaat
    10201 ccagtacgct tcagacggtc aaactgcgat ttttgaaatg gcagaaatcg tcggattgaa
    10261 agatgtccca ctaaaccaac aacagccact taggttatcc acgcgaggag taggagaact
    10321 gatacgacaa ttgttgcaac aaggtgttcg acacatcatc atcggcgttg gcggaagtag
    10381 taccaacgat ggcggcatcg gcatggcaga aggtctaggt tatgcttttt acgatcaaga
    10441 taatcaaaaa gtagaagcac tgggagaaaa tctaggtaaa attgctacag ttgataaaac
    10501 gaacgtgcct attgagctga ttaaagccaa aatcactgtc attactgatg ttactaatct
    10561 tttatgcgga aaagaaggcg cttcttttgt tttcggacca caaaaaggct tgaaagaaaa
    10621 tgaattagca gtggctgacc aagccatgca tgatttttat atgagatttg cccctcaagt
    10681 aatcgaggca tcgggcagta gtgctggtgg tggaatgggt gcaggactgt tagcatttgc
    10741 ttcagggaaa atagtctctg gcatcgacta cgtattggat ttactggagt tcgataaacg
    10801 ggtacagcaa gcagacctcg taatcgtagg cgaaggacgt ctagatggac aaagtctaaa
    10861 aggaaaagcg ccagttggga ttgctagaag agtaccagag ggcgttcctg tgatagctat
    10921 ttgcggaagt gttggtaaag gaagcgaaaa agcagcagat gttggcattt cggccgtttt
    10981 tccgatcctt cctgcagctg tgcctttaca agaagcttta cagcaagcca ctgataatct
    11041 tcatcgcaca gctaaaaaca tcgcatcttt acttcaagtc gttcattttc aaggaggtaa
    11101 tcgatgaaaa tcaaagcagc actattcgat ttagatggcg ttcttgtaga cacagctcgt
    11161 tatcattacg aagcgtggct ggttttagct aaccagctgt ccattccatt cactgagaaa
    11221 gaaaacgagc aactgaaagg aatatccaga accgagtcac tagagcgatt gctgtcattt
    11281 ggaaaaatgg aacagaaatt ctcagaaaaa gagaaatcag cctttgctga gcaaaaaaac
    11341 aatctatatc ttcaagcgat tcaaaaaatg gatgaaacat ccgttttgcc tggtgcaata
    11401 gctgttcttg agtacttgaa aaaaacaaac atcaagatcg gattgggatc agcaagcaaa
    11461 aatgcacgcc tcattttaga aaaaacgaat ttgacaagtt attttgatgt ccttattgac
    11521 ggcacgcaag tatcaaaagc aaaacctgat ccagaagttt ttctcaaagg agcacagcaa
    11581 ttgaatgtac cgccaaatgc atgcttggtc attgaagatt cagaagctgg ttgtcaagca
    11641 gctttggctg gtaacatgca tgtattggga ataggtgaaa acataaatct tccttctgct
    11701 gaatatgtga ttcctgattt aaccgttttt gatcaagtac gttcgttttg gcatttatca
    11761 gaagccgtat cacgtatata atagtttccg ttttttctgt cacaagcaaa acgttatcct
    11821 ccttttggaa atcaaggtat gatgagaaaa aagggggatt ttatatgaca aaaaaagcag
    11881 ttttcgcagg cggatgcttt tggtgtatgg tcaaaccatt tgaagagcag ccagggatca
    11941 tttctgtgac atccggttat actggcgggc atgtaccaaa tccaacttat gaacaagtaa
    12001 caacaggcac aacagggcat acagaagcag tagagatcga atatgatcca gaagttattt
    12061 cttatgatca gttggtggaa atctattggc agcaaaccga tccgactgac gcattaggac
    12121 agttcgctga tcgcggtgat tcttatcgtc cagtgatcta ctataatgat gaagaagaac
    12181 gaaatattgc agagcgttca aaacaagcat tgcaagacag cgggcgattc gataagccaa
    12241 tcgtgacaaa aatcgaacca agaagcgaat tttatcctgc agaagattac catcaggatt
    12301 attacaagaa aaacaagctc cattacaatc tttatcgaga aggttctgga cgtgcaggtt
    12361 tccttcgtcg acattggggc gtcaagaaat aagaagccga aaaagctact tatggaacac
    12421 cgtatatcag aggttgtaaa accagcattt tgagctaata ctgagggtca gctgattgaa
    12481 caccatttag tggagaggtt gtgaacctgt cgcctagctc caagtcacaa ggatttggta
    12541 agagaacact atatatcaga ggttgtgaca agtcgtttgt cacaaccttt tttcaatacc
    12601 taagatgaag gacaatcagc aacgcactgc tcaaaaaagg aatgaaaggc agcttttcca
    12661 actttttttt atgacagata aagtaaacag aaaaaacgga tattccaaac agactagcaa
    12721 gaaacaagat ctgaagcaac tcttctagag acaagccacc actccaagaa agcatcaaca
    12781 gagtatcgcc agctccgaga taggattgac catagcgaag gcacacaccg cctacgataa
    12841 acgttaacag cacactttgc cattggaaga cgccggcctg caaccaaaaa atccataaaa
    12901 ttgcccaaga acagaatagc gtgtgagaat ggaccatgaa gtaaaataaa tccatcaacg
    12961 aaaatagcaa agccgaaagc atccatatga acgtcgaaaa atttcttgcg gaagaaaaag
    13021 taaagaacca ggcaaaaaga ccaccacata aggcttctgc caacaaatag tagaagggca
    13081 accgacacct gcaatagcgg cagcgaaatc gttggacgat gatcgagaga atcggaatca
    13141 attcatacca agaaagcgaa cgacagcatt tgacacaatg agatctagga taaataatag
    13201 agtgacctaa cggaatccgc tgggcggtta aacataaaaa tgatccgata cagcaaccaa
    13261 tgataaaata aagtaacacg ccatctcccc cttttcaaaa ccaagatacg caagcttaaa
    13321 ctaaattttt gtacatacat aaaatttgag gtgaaaaaat gaacgtttca atcggattag
    13381 atatcggaac cacacaaacc aaagcagttg ctttcacaga tgaagcccaa gaagcagcat
    13441 ccgcttattt tcgctaccct ttgatccaag aaacacaagg gatggcagaa caagatcagg
    13501 aattgatctt tcaaggtgtc tgcaccgtta taaaagaagt aattaaacag ttgccttcag
    13561 acacaatcat tcaatttgtc tccttctcct ctgcgatgca tggcctgata ttgttggatg
    13621 aagagaaaac accgttatct cgaatgatca cttgggcaga taaccgagca gcggacgaag
    13681 cagaaaaact gaaatatacc gtgcttggca aagaatttta tcggcagact gggacgccga
    13741 ttcatccaat gacaccattg aacaaaatca agtggctgca aaacagccaa cctgaattat
    13801 tcaaaaaaac tgcttatttt gtcggtatca aagactatat cttttataga ttgtttggcg
    13861 aactgatcag cgactacgga acagcttcag gaactggata ttttgatatc catgaattcc
    13921 aatgggcaga agaagtgctt gactacttga acattcgaat agagcaattg ccgcaagtct
    13981 ttccttccac ctatcaagta acaggtctac catcaaaaac agcagcagaa ctaggtttgc
    14041 caaatgatat atcatttgtt ttgggagggg cagacgggcc gctttctaat ctagggttag
    14101 gtgcttttgg tgatacgatc gccgctttga ccgtaggaac gagcggagca cttcgtttta
    14161 ttactactca gcctcagttc catccagaaa tggagacgtt ttgttatgtt ctggatcaga
    14221 aacattgggt agtgggagga gcgactagca atggtgctgg catctttgat tgggcagggc
    14281 atacattttt acaggaagtc gttcaatctg ctaaagaaaa aggagaaaat ccatatgatg
    14341 cactgctttc ggctatcgct tttgtgccag cgggtgccaa tggtctgatt tttcatccct
    14401 atttacttgg tgagcgagca ccgctttggg acgcagatgc gtttggcagt tttatcggtt
    14461 tacagcggca acatacagaa aaagaaatga tgaaggcgat tctagaaggg atctgtctca
    14521 acctttatcg gattttaaaa ggagtttgcc aggaaaatca gctcacagag atccgtgcta
    14581 caggcgggtt tgctcaatct ccgatttttc gtcagatgat ggctgatgta ctgggttacc
    14641 cgcttgtttt ccctactgtc acagaagcat ccgcattagg agctgtgatt ttaggctgga
    14701 gaagcatcgg aaagatccaa agtatcagtg aagcacagga attgatagaa ttgaaagagc
    14761 aagttggagt acaagaaact gatcataaaa aatatcagaa aatctatcca ttatttgttt
    14821 cgacacaaaa gcatttagca cagacttatc agcaatttgc tcaactgcga gaagaattag
    14881 ctgattctga aaaatgattg gctttacgga taaaaaagcg aactgaaaat cgttctcagg
    14941 taaatgattt tgttgtactt ttcattgaaa gtctactatt attttaagtg taagaaaatg
    15001 ttaggaggca agaaagatga aattccaaca aaccaaagaa gtattaaatc aattagttgc
    15061 agatctaagc caaatgtctg ttgttgttca ccaaacacat tggtacatga gaggaccagg
    15121 tttccttaca ttgcacccaa tgatggatga gttcatggat gatttgaacg ctcagttaga
    15181 tgaagtatcc gaacgcttga tcacattaga cggttcacct tattctacat tgcgtgaatt
    15241 tgcagataac acaaaaatcc ctgatgaaat tggccgctgg gatcgtacta ttccagaacg
    15301 tttggaaaca cttgttgcag gctatcgtta tctagcagat ctttaccaaa aaggaatcga
    15361 agtttctgga gaagaaggcg acgatccaac acaagatatc tttatcgaat tcaaaacagc
    15421 cacagaaaaa cgtatctgga tgatccaagc tcatttgggc aaagcgccag agatcgacgc
    15481 gtaaaaactg ttgttgaact tgaccgaaaa agaggttgtg aaatcagcgt tttgacctga
    15541 gtattagaga aacaaacgtc aaaatagttc ctcatatttt gccgtttttt gagctaatac
    15601 cgagggtcag ctgattgaac accgtttacc aagaggttgt aagcctgccg ttcagctccg
    15661 agtcacaaga aacaatttta aaaaacagct tcttatgttt tatggatatt gtgacttgtg
    15721 accgaggagt tggcaggaga acaccgttta ttcaaaataa ggggttgtga caagactttt
    15781 gtcacagcct ctttttgttt ttcattttga caggaaacga cccttttata taaaagaaag
    15841 aaaagaagta aagtttcata taaatttttc aatcaagcag aaaaacgttg acattgacta
    15901 gattagcggg taatatgtta aatggtattg tttgccccac aaagagcccc ggaaactcga
    15961 agtgtatgtc aaacatcaga acttaaattg gaggaaaata acgtgcgtac aacatatatg
    16021 gccaaaccag gcgaagtaga acgtaaatgg tatgtagtag acgcaactga tgttccattg
    16081 ggacgtcttt caacagttgt tgcatctgta ctacgtggta aaaataaacc aactttcaca
    16141 cctcatttgg atactggcga tttcgtcatc gtcatcaatg cagacaaagt gaaattaaca
    16201 ggtaaaaaag cgactgacaa aatttattac cgtcacagca actacccagg aggattgaaa
    16261 tcaatcactg ctggcgaatt gcgtgctaaa aattctcgtc gtttgatcga aacttctgta
    16321 aaaggtatgc ttcctaaaaa cactttaggc cgcaaacaat ttacaaaatt gaacgtatac
    16381 ggtggagcag aacatccaca tgcagcacaa caaccagaag ttttagacat cacaaactta
    16441 atctaatagg agggaatttc attggcacaa gttcaatata tcggcacagg ccgtcgtaaa
    16501 aacgcagttg ctcgcgtacg tctagtacca ggaactggca aaattactgt aaataaaaaa
    16561 gatgttgaag aatacattcc acatgctgac ttgcgtgaag taatcaatca acctttcgct
    16621 gttactgaaa caaaaggcgc atatgacgta tttgttaacg taaacggcgg aggatacgct
    16681 ggacaatcag gagctatccg tcatggtatc gctcgtgcat tgctagaagt tgatcctgac
    16741 ttccgtttag ctcttaaacg tgctggctta cttactcgtg acgcacgtat ggtcgaacgt
    16801 aaaaaaccag gtcttaagaa agcacgtaaa gcttcacaat tctcaaaacg ttaatatcct
    16861 ttatatatca acgtttctgg cacttttcaa ttatttggaa agtgcttctt ttgtttttaa
    16921 gaacacattt aagaacacat atcaatcaca cttattcaat cctctctgaa aagcttcaaa
    16981 aacagcctta tcaactatat ctggtgtgtt cacatatatc tttgttgtag aaatatctga
    17041 gtgtgttaaa gcatttgaaa tttgattcat atctgctcca ccttgtctag ctatagttgc
    17101 ataagtatga cggagtttgt gtggagataa atgaactaag tcaggatgcc ttcttttaat
    17161 tgaattgatt cgatagttta ggtaatcggc atgaacagga caatttactt ctccagatgg
    17221 tttagtataa gtaaagagat actgatctaa cgtttgctta atttttaatt tcaacaattg
    17281 atcagcttgt accgatttcc attcacttag caatgtcatt acaacttctg gtacttgaat
    17341 gacggtatct tttcgacctt ttgtaaactt ttttctttga tatttatcta aagtatgttt
    17401 aagacggacc gtttgttttt caaaatcaat atatttccat tgcaaagcgt aagtctcact
    17461 ttttctatca ccaagataca gggtaatcat aaaaagtgtg taatcatgaa aagtaagtaa
    17521 atgattatta aaatcatcat ttactgcttc tatccaatta gtaagttctt ttgatgatag
    17581 tgcttgctta ccttcttgta ttcttttttc ttctaaagca ttttttttag gtgcagtgat
    17641 actttgaata attttagtag ttcga
//
LOCUS       DAJSKS010000052        17272 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJSKS010000052 DAJSKS010000000
VERSION     DAJSKS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.053"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(8..553)
                     /locus_tag="ON396_001452"
     CDS             complement(8..553)
                     /locus_tag="ON396_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3113543.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(565..1257)
                     /locus_tag="ON396_001453"
     CDS             complement(565..1257)
                     /locus_tag="ON396_001453"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR3113544.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1276..1560)
                     /locus_tag="ON396_001454"
     CDS             complement(1276..1560)
                     /locus_tag="ON396_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113545.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1573..2136)
                     /locus_tag="ON396_001455"
     CDS             complement(1573..2136)
                     /locus_tag="ON396_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR3113546.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2364..3026
                     /locus_tag="ON396_001456"
     CDS             2364..3026
                     /locus_tag="ON396_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCR3113547.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3150..4346
                     /locus_tag="ON396_001457"
     CDS             3150..4346
                     /locus_tag="ON396_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCR3113548.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4447..5823)
                     /locus_tag="ON396_001458"
     CDS             complement(4447..5823)
                     /locus_tag="ON396_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3113549.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5860..6726)
                     /locus_tag="ON396_001459"
     CDS             complement(5860..6726)
                     /locus_tag="ON396_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HCR3113550.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6815..7840)
                     /locus_tag="ON396_001460"
     CDS             complement(6815..7840)
                     /locus_tag="ON396_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3113551.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7858..8532)
                     /locus_tag="ON396_001461"
     CDS             complement(7858..8532)
                     /locus_tag="ON396_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3113552.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8716..9609)
                     /locus_tag="ON396_001462"
     CDS             complement(8716..9609)
                     /locus_tag="ON396_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3113553.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9683..11380)
                     /locus_tag="ON396_001463"
     CDS             complement(9683..11380)
                     /locus_tag="ON396_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR3113554.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11373..12686)
                     /locus_tag="ON396_001464"
     CDS             complement(11373..12686)
                     /locus_tag="ON396_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR3113555.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12686..16723)
                     /locus_tag="ON396_001465"
     CDS             complement(12686..16723)
                     /locus_tag="ON396_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR3113556.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16736..17194)
                     /locus_tag="ON396_001466"
     CDS             complement(16736..17194)
                     /locus_tag="ON396_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3113557.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4840 a         3889 c         3009 g         5534 t
ORIGIN      
        1 tgttttttta taaaatttct gcgcctagtg tggactcaaa atgtctcaat gcccattcat
       61 gtccaacggg atcgaaactt gccacggaat ctttgaaaat atggagtttg tatcctagat
      121 tatacgcatc gactgctgta tgcaacacac agatatctgt acatacgccg gtcaaataaa
      181 tatctgtgat ctggcgttct cgcaaacgga tgtctagatc tgttccacta aaagcggaat
      241 aatgccgttt gtctatccaa taaacatttt cctcttgctc atagtcttgg tataaacttt
      301 ttaaagagcc gtacaattcc cgtccacccg ttcctatcac attatgcgga ggaaataagc
      361 ggttctccgg atgaaaggaa tcttttggat catgcgcatc aatggcaaaa acgacaaaat
      421 ccttttgatc aataaacttc tttgtatgag cagtcagctt tttttcaatt acttgtcctg
      481 ctgtcccagt agtcagcttt ccatcctctg caacaaaatc atatgtgtag tcaatcgaga
      541 tcaatgcgtt catcttagtc ctccttatac gagatgtttg acaaattcga tcaccatttc
      601 tgctgatcgt ttgcctgcgt cttcgataaa ttcgtcaaag ctttgtgtcg ccgagtgatc
      661 tgctgtgtcg ctcattgcac gtacgatcaa aaatgggata ttgaactgac gagctgtttg
      721 tccaacagcg gctccttcca tttcgcaagc taatgcttca gggaaattcg ttaagatttc
      781 tttgatttta tctggcgagt cgacaaatgt atcacctgta acgatcaatc cttcttttgc
      841 gttcaaattc gttgcttttg ctgctttaac catttcactg cgcaaatatg tgcttgcttc
      901 ataatataga ggcattcctg gtaattgtcc tgggttgtag ccaaatcctg ttgcatctgc
      961 atcaaaataa gctactttgt ctgatataac gatgtcacct acttgtaagc cttcaccgat
     1021 tccgccagct gaacctgtat tgatcaccat gttcacgccg tattgttgga tcaacagact
     1081 tgtggtaata gatgctaaga cttttcctat accggaacgt acgacgatca cttcgtggtt
     1141 acctaatgaa cctgaaataa ataaagcgcc tgcgcgttcc catgataatg gttcgcttag
     1201 gttctctctc aaaattttta cttcttcttc cattgcgccg ataataccga ttttcatctt
     1261 tttaattcct tctttctata taaatgcgat tgctaaaaaa accacacata acattaggat
     1321 aacaataata agagatttca tcaaaaaaga gttccatttg gtcgtctttt cccgttcgct
     1381 gatccttgtt ttcgtgattg gtttattctt tttgctttct ttacgataaa agctggcaat
     1441 ctttttttct tcttgctgat aagctgtttc tgcttttcgc tgttttttta atgactcgct
     1501 tgcttgtgct tgttgtcgct tgcgaagttc gcttcgcgta ataagcggtc ctttggccat
     1561 aagccgatcc ctctactttc ctttggtcaa tagttcccag tattgaatcg cataaatcgt
     1621 ctttgcatca caaatcaact gatctttcat cgcttgttgc gcctcttcaa gagttagatg
     1681 atacaactcc agtacttcgt cttcatcttg tgctaaagga ttctcgacct tctctacccc
     1741 ttgtgcatga taaatatgaa ggacttcatt tgcaaaacct ggtgaaagat acatcgaagt
     1801 aagatggctc agcgacttcg cacgatagcc agtttcttct tctaattctc tagctgctgt
     1861 catttcggga ttttgtcctt ctcctggatc gatttttcca gctggaatct ccaaaatcac
     1921 tttttctaaa ggttttcgaa attgtttgac aagaagcaga cgatcttgtt cgtcaaaagc
     1981 aatgatcccg acaccgccag gatgaaatac cagttctctc ttcgaagttc ctccatctgg
     2041 caaacgaact tcgtctacag caacatcaat aatttttccg ctatagattt ctttgcgact
     2101 gattgttttt tcttcaaatt gctcattgtt cagcatatct tctcctgctt tctttgtaat
     2161 cataactgct tttttttcat ttttcaattt ttctcaaaaa ctccttatta gtttaccata
     2221 gataaacctt agattttctc taaatataga aaatccttct ttagtccgat actctttcta
     2281 gtagtccgta tgctaaaata agtatgtaat cgaataacga aaagaattca atactcaacg
     2341 ataaatgaca tggagggttt attatgataa aacgaaattg gccgtggctt ctagcattgg
     2401 tcttgctatt tctctttgtc cgaaacaata gtttttcttt tgttctagga cttttattga
     2461 taggtgtagt tttagttttg tggggactct ttggttcgcg caggaaaaag ggaaaacaag
     2521 aagaaattcc tgcactgact gacgaacttg aaactcacta tgaaaaaagc ggtatgacag
     2581 cgaatgaaat ttcatttttt cgacaaacaa tgaatcaaac aaaaaatgag atcaatcaat
     2641 tgcaaatgaa tatgcagcag acagccaaac ttaaagcaat cgatctccgc catgatccag
     2701 tcaaagcagc gaaagcttta ttcaaagaat tggtgaaaga accaaaccga ctgcatgaag
     2761 caagtcagtt tctttatacg catctgccga atctagttga tttgacaaat aagtataatg
     2821 agatcaatga ccatgagatc aaaaacaaac agacctatga aaaactagaa gaaagcgcgc
     2881 agatcatcga ccagctgtca gctttaatcg cacaagatta tcaaaaattc gttgctgagg
     2941 atctagatga tatcgacgta gagatatctg ttgcgaaaca gagcttaaag cgtgataata
     3001 aatatgaacc agaacaaaag gattaactct tttgttcata aaagaagact cggaacattc
     3061 agatgatgtc tggctgtttc gagtactttt tctaaagtag agacattcat gtaagaatcg
     3121 agaacagatt caacaaggag gaatttttta tggatcataa accgactgaa acaaaagatg
     3181 ttacaccagt taacgataca cttgaagatt tattgaacaa cccgttttca actccaacag
     3241 atacgctgac cacttcacaa caagcggaga ttgatcagct ccaagagcaa cagacagctg
     3301 ctcgtttaat tgacaaacta ccggcggaac gccaagaaca agctaaacag ctagcagcaa
     3361 aaatcgatgc taatgatgca cagtctgtca tcagctacgg atcggctgcc caagcaaaat
     3421 taagtgaatt ctcacaatcg atgttgaatc atgtgcaagc acaagatatt ggtcctgtag
     3481 gagattcctt aactgaactg atgtatcgtc tccaagaagc caatcctgac gaactgcgtg
     3541 ccggagaagg gaacatcttc cagcgtgtct tcgggaaggt caaacaatcg atctatgaag
     3601 tgacagcaaa atatcaaaag attggtgcac aaattgacaa gatttcagtg aaattagata
     3661 aagaaaaaga cggtctgcta aaagataatc tgatgttaga acagctttac cagaaaaaca
     3721 aagactactt tgatgcactg aatatttata tcgctgctgg agaattgaaa atggaagaac
     3781 ttcaaacaac gatcattcca gaagcgatga aaagagcaga agaaactggc gatcagatgg
     3841 atgttcagat tgccaatgat tacacgcaat tcctagatcg tctggataaa cgtacgcatg
     3901 atcttcgctt agctagacag atcacgatcc agcaagcacc acaaatccgt ttgatccaaa
     3961 acacaaatca ggccttggct gaaaagatcc aagcatctat cgctacagcc attccgctgt
     4021 ggaaaaacca agtcgtgatc gcattgacct tgcttcgtca aaaagatgca gtaactgctc
     4081 aacgccaagt ctctgaaaca acaaacgact tattgaagaa aaattcagaa atgctgaaaa
     4141 tctcagccat cgaaacagcg aaagaaaatg aacgtggaat cgtggatatc gagacccttc
     4201 aaaccactca aaatgatttg atcgaaacaa tccaagagac attgcgtatc caaaaagaag
     4261 gaaaagaaaa acgtcgtcat gcagaagtag agcttggaca catggaagaa gatctgaaac
     4321 aaaaactgtt ggatttaacc caatagatca cctttcgctt gttactaaaa caaacgccca
     4381 acaacagttt attacaactg ttgatgggcg tttttcttat ctttcttcta acttctccat
     4441 tggagtctat ttctcattca tcttgctgtg tccatagcca tagccaaaat aaatgatcag
     4501 tccaataacc attacgatac cgaacacgat ccatgtcaca gtttgcagac gaagcatcaa
     4561 tacaatacaa gtcaaaatgg agacgattgg caatagcgga acgaatggca ctttgaattc
     4621 tccagctttt ggtttgccaa acatttttct caacttcagt aagcctagac taacaagcat
     4681 caaataggcg agcgccatga tattcgttag ttccgctaat acattcaatg ggaaaaatcc
     4741 ggcaaagaat acagctgcca caccagcggc ataagtcgct tttttcggtg tacgatgctt
     4801 ttgatcgatt tctgtcataa acttaggaag taagccatcc ttgctgacag caaacagtaa
     4861 acgagcaagt gaatacatca tcgagatcgt taccgtcagt agcgttaaaa ttgcccctac
     4921 agcgatcacc gcgcccactg ttccatgtcc tacatacctc attgcaaaag ccactgggtc
     4981 attgactcct agttcagtat acggaacgat tcctgtcaag atcagcgtca cgatgatgta
     5041 aaggacagta gcaatcagta tcgaaccaat gattcctctt ggcacatctt tttgcgggtt
     5101 tttgacttcc tcggctgcca ttgcaacggc atcaaatccc aagaaagcaa aaaacacaag
     5161 agctgcacca tctagcacac cggaaaaacc aaaaggcatg aatggctgcc agttatcagg
     5221 tttaacgaaa aatattccta caatgataaa caacgcgatg ataccaaatt tgacatatac
     5281 catggcatta ttcaaacgta atgctttctt ggcttcaagc gacacgatgt aagtgacgaa
     5341 tatcactacg agcaccgcaa tcaagtcaat gtatgtacca ttttccggat tgtacccgcc
     5401 agttaatgct tgtggaagtt cggcccctaa acttttcaaa aaaccttgca tatagccaga
     5461 ccatccggaa gcaacagagg aaaccgctag taaaaattcc ccgatcaaca accaccctgt
     5521 cagccaggca gcaaattcac caaatacaac atacagatag gcatatggcc caccaatcac
     5581 tggcactcgc gaagcaaatt ctgcgaaact tagtccagac aaaatgacaa cgattgcagc
     5641 taagacaaat gaaagtgaca gtgctggacc agcattttga ttggccgcaa cccctgtcac
     5701 cacaaatatt cccgtaccga tgatcgcacc aattccaagc atgatcaaat cagacgtttt
     5761 tagttctttt ttcatctcac tcgtatgatt gagattgatc tctttcttac gaaacaaatc
     5821 cattgaagtt cctccccatt ttaggttgga aaacagcgat taaactgttt tcatataccc
     5881 taaaaggata ccacctaaaa tgactaaaag gcaaccaaca ataacataaa tgagctcttt
     5941 tttcgttttt ctttcgccaa ggatgaaaat cccacctaaa gtagagataa tgatccccat
     6001 ttgtgaaagt gagaagccaa cagctaaacc aagtgatttg actgttagaa gcatgcagat
     6061 gtttcctaag ccccataata atccacagat catgtttttc cacacaaact gatcgacttt
     6121 aactttacgg aaagcaaaga agcttgcgcc taagatcatt ccgatacttt gaggtaagat
     6181 aattgacatc gcatccaaat tcgcccagtt gacgatgatg gtatatacac cataaccaat
     6241 cgtagagaag atcaatgctc taaaaccctt accaaaatca agcattttat tatctgtatc
     6301 tatttttcct tcgtcatctt gtcgggcagt caaatacgca ccgcttacaa gaagagctaa
     6361 agcaataata ccgtatacat aatctttcgt ttgtgtccat tcatggaaga aaacggctcc
     6421 cgcaatcgta ttcaaaatta attgaaaacc agtagacaat ggtagcccta cagaaacacc
     6481 catgtacttc attccatgga actgtccatt ttgtccaaca ctccaagcta agccggataa
     6541 aaatccgatc aagaaaatcc aaggggtgat tacaggacta acaacgaaaa agacaactaa
     6601 agaaaacaga aatgcaccga tcgtcattcc tagtgtttgt tgattggcat cgccaccaat
     6661 tttaccactt actaaaccaa tactacccca tgccaacata gggactaatg ctattaaaat
     6721 ttccataacc aatctccttt tatttcacat gataagaaac ttatcagcga aaacgttaac
     6781 aacacaagca ctttttttcg tttctaacga ttttctaacg tttttcatta taaaaattta
     6841 agattgggaa aaataaagtc attgttgtcc cttttccttc agttgattca gccgtcaaat
     6901 ggatacctaa tttttcggcc agattttttg ctaaataaag tcctaatcct gttgaatgtg
     6961 ttttgctaag tcgaccattt ctcccagtaa atcccttatc aaagatccgt ttcaagtctt
     7021 ccgctgggat accgatccct gaatctttaa ctgaaaggct gactccttgc gcttcctttt
     7081 ctatgctcac aatgatgttt ccgtgatccg gcgtatattt gatggcatta ctaagcaatt
     7141 ggttgaagat aaaagcgatc catttagcat cggtcaaaac agtatgatcc tcaccaacga
     7201 tctcgtagcg aatatttttt tggataaaat aatttgcctg actgcgtatc acaggctgaa
     7261 tgattccttt tagtccatat tcttggatga ggtagtcccg cgagaaactg tcgagtcttg
     7321 cataatacaa aacttgttct acgtattcat cgatttttga taattcattt tctagtaatg
     7381 tatacttttg atcgtcaata tcgaactcga tcgaacgaac tagcaattta gaagcggaaa
     7441 gtggcacttt gatttcatgg acccatgagt caatataatc tttttggtct tgttgattac
     7501 tgattgctga ttgcattact tcttggtgtt cacggatcag ctgattgata tattcttcta
     7561 ccagtttttc ttcttctgtc ctcccaccac tcaaataatt ttgcaaaggg gaagtttttt
     7621 gtatgtcggc taattttccc caccatcttc tcttgctcaa gtaaccaccc agcagataaa
     7681 aaatcagaaa gacgccctca atcaaagcta aataagcaat tgtgctcaaa tcaactttga
     7741 tgtttggcgc cagccacatc acaaagatcg tcaatgcaat aaaaaacagc catcccaaga
     7801 tcaatgtcca ctgatctttc aaatatttaa aaaaattcat cttaaccgct catcctttca
     7861 gggaacgata tatccctgtc ctacttttgt ttcgatatat ccttggatac ctgcctgttc
     7921 gatttttcgg cgtaaccgat tgatgttgac tgtcaatgta ttgtcatcta caaatcgttc
     7981 gtcatcccac agtgctcgca gcaatttttc ccgacttaag atctttccat tgtgcttcat
     8041 caaaataaat aatagacgat attcattttt gctcaaatca atgatctctc cgttgatttc
     8101 catacttccg ttgtcaacat tcaacgtaat gccattgtgg ctcatcattt cactagataa
     8161 ttctgtgtag ttgtaagaac ggcgaagtaa tgcattgatt tttgcaatca gtacatcgat
     8221 ttggaaatgt tttgtcacaa aatcatctgc ccccatgttc atagccatga tcatatccat
     8281 attcgtattc ctgctggaga taaaaatgat cggtactttc gatacttcac gtattttttg
     8341 acaccaataa tagccatcca aaacgggaag attaatatct agtaatacaa gctgtggttc
     8401 ttctctttca aagtctgaga acacttgatt gaaatcggtc gttccaaagg tttcaaattg
     8461 ccatttctca agttcctcca tgatcaattg tcgaatgatc tcttcgtctt cgactaccat
     8521 gatttttgcc ataaaaaatc cctccatctt tcttcttgct actacaatat caaagaagcc
     8581 cttgtgaaac aagttggctt acctggaaaa tgtcatttta gcaaacgtta aaaaaaactg
     8641 gaaatatgcg atttaatcgc gtttgtctct tccaagcagc ttaaatttat tatgttattc
     8701 ttaataattg gtctattatc tgttattttt ctttcgttgt ttttgtcttt taaattttcg
     8761 ataacaccaa taaccgaaaa gcgttagcac tagcaatata cctagaagga gtataagtaa
     8821 tcgataaaga tggtattttt ttgtctgttc tatctttgaa gagacttctt ctgtaaaggg
     8881 aactcgtctt cctgtcacta gtaatcggtg agtgttcacg gtgtaaggtg tacaagtgat
     8941 aagtgtgact agatcctgct tttctctaat tgctaatgta tttgtttttg ttggaaggac
     9001 aacaattttc tcaacgactt cataagcgag catccgacca tacacttcaa gatagaatcg
     9061 gtcatcttgc tccatttctt caagatccgt aaagagtttt ttctcagcaa taccgctatg
     9121 ggcggtaatc acggaatgtg tactctctcc tccagttgga aaagaggtgc cttgcaggac
     9181 tgtcgctcct ctatctaaca aaagatcatt cgtttcatca aataaaggca agctaacatt
     9241 gattttcgga atatagatgg ctccgatcat atgttgttcg taatattctt ttcctggatt
     9301 ttggacattt ctaaccgctt gctcaaaagg atctttgact tgtcccattc ctgggattac
     9361 tgatgtatga gcaagcgtct gatttttctt ttcatgcact gctaattgtt gctcatgttt
     9421 ttcttcttta tcgcgttcaa gctgcttttg ataattctcg atccttcttt gatccaaata
     9481 attgctgaga gcatctgcta aaaatggata tgtaaaaact gcagctccgg caagaaacat
     9541 cactgccata aataattgta ataaaagact gatttgggtt tgaggtctat tacctatttt
     9601 ctttttcatt ttcttcacct tcttatttaa aaaaactgca gcaaccttta taaaaccgat
     9661 tgctgcagct tatttttgtt gcttatactt cagacttcat tttagattta cggtaccagc
     9721 tgtacgctcc aatcatcaaa cttattccga ttagtaagaa tgccaagagc ccgttcccac
     9781 cagtagcagg aagtgacccg cgtttcatat tcggtactct ttctggccct ggattttcta
     9841 ctcctacgat ctgaatttct tcattatagc ttccataaga gatgataaat tcagtaaagg
     9901 ctgcctcacc tgttggaagt acataacctt ctggcgccat ggtctctttc atttgatagg
     9961 ttccataatc taacccttga acctcaaagt atccatttcc atctgcgaca aattctgttg
    10021 cttgtccgct tgttgcccac tcaattctat ctgctcctga ttcataaaca gctaaaggcg
    10081 actccccttt ataaaatatt gcgtattcag aacggtcttc attccacaat tcgaatcttg
    10141 catctttaag caattcactg cttgatgcat catatttttc aaactttctt ccacctgttg
    10201 tgactttctc tggcggctct actccttctt cataatcaaa ttcatcattt cgtccgattt
    10261 caataaccgc tgtgttgtta tggaaatcat ccgcttctag ttctgtggaa accttcaaat
    10321 tatacgtgat cgttaaatct cctccacgtg catctgctaa tctacctaaa gtatctactt
    10381 tggctactcc attttcttct gtcaaaatcg tcacgacaaa cccgttgctt actacttcta
    10441 ctatataatc ttgtcctttt gttaaaatcg tctcgccggc tcgcacttct atttcctgat
    10501 cataataagt tagaccaact gtaggcgtat ctgtgatttt gtaatttttg ataacagcgc
    10561 ttccgttttc aaggacagaa tcgatgaaat aaggaatagg tgctgtgatc gtgtaagaag
    10621 ccgtttttcc tctttctaga tcaatataag aaatcgtttc ttgttctcca gcttcattaa
    10681 taatcgttac ttgtctaagg tcgctttcct ctacattcat ctgtttatct gcttctctga
    10741 tttcgttttt cggatacaaa tgcacatctg tattgtatgt gtctttccat gtagaaccat
    10801 cccacacgcc atccaccata tcaggcatca tcactggcat cataacaacc attggagacg
    10861 cgattctgga aatatgagct ggcgaataag tttctaagaa aagatagact ttatctctag
    10921 aagaaggtgc ttcttccgtc acttggacgg tagagaatac cgcttctcca gcttcattcg
    10981 ttgtttgttt gtcaatactt gtcagtcgat attttaaaaa ccaactttct gtgtgccgtt
    11041 tttgaatcca ttcaatcgct tcggcagaat taagaccgtc ttctcgttcc ggtgttaaag
    11101 gattatctcg aatcaaatcg taatatactt ccgttacgtc aaacaaatca aaatcgactc
    11161 ctggaatatt ttcttcacca aaatccgtag aaaccaatcc gctattcggt cgttcttccg
    11221 gaacagatga gaaacctttt ttgtgtaatg taatcgtcac ttcacttggt ttttgtgcga
    11281 cttcttcggc tttgactagt tttccgttcc cgaaaatccc tattcccgca gtaacgagta
    11341 acagtacact cattcccgtg acaatcattt ttttattcat ttttttctcc ccgttctttt
    11401 tttattaata aaacagcacc taacaataaa agaattccta gtaaagaaat caacgttctt
    11461 gcttcattcg tttgcgggaa tacggaccat tttcctgatt gacctggatt gcttggacca
    11521 ctcggctctt tcggtttgtc cgttgtttct tttacggtct cgttataaac acgtggtgtt
    11581 gcggattcat aggcagagac tggaactttc ccggattcta attcagccat ctcctgtccg
    11641 ttaactacta catattgtgg ttggcctttt gcatccaccc aaaattcagg gatgatgacc
    11701 tcgattctct tacttgcttc atccatctcg taaccttcca caccttttaa ttcttcaaaa
    11761 taataggtgc cagaaggcaa gaaacgttgt cctgtcgtaa caaggccctc tttatccgaa
    11821 atgaatttgc ttacattttt gtcattcaaa ggatcattgt ctgctggttt gatccattga
    11881 tttttcaagt cattagcagg agccatatct aaataatatt tccccccttc tatcatctga
    11941 tatagagcaa aaacagcacc ttctaatggt tttccttttt cagttgttcc tctttgacgt
    12001 ccatacttga agaagtatgg atctcttaga taaccgacat ttttaggata gatatgaatc
    12061 tcttgcagtt cctcggttga ttctgtcggg tgataaattg gcaaaatagc agcaattggg
    12121 atagctgtct gatcgatatc aacatccaat ccaacttctg gatcaatcga ttcttcgaaa
    12181 atcaaataaa ctgcatcccg atcttggttt ttcttaggtg ctttgatacg tgcaatcccg
    12241 tctcctaatc cgccaaccgt atctttccca gttgttaccg aagtccctat ttggacaagc
    12301 ctttcttctg tgattagctg acggatattg tttctgttcg tttcagcgat ttgctggacg
    12361 aattctttag gagtcatgtt ttcagcagct tttttttcat gataataatc ggtcatgtcg
    12421 taaatggcaa atgtcgctcc atttagtggc accgtctgtg aaatgatatc tgctgtttcc
    12481 cctgcttcct cagagatagc caagccatca ttttcatggt attcaaattg atcgttataa
    12541 tcgatatctc gaatcatccg tttatgcaac acgaattcca cgacatcttc ccctgattct
    12601 tctgccgaca tcgcatgtgg aagaaggact gctgcaagcg gtagtaaaac aagtaattga
    12661 agcatcaaac acaacagctt tttcattacc ttgctccctc ctttttagat tgtctgacaa
    12721 acaaaccata ggctgctcct gcaagcgcca tgaagaatgc agcaagcagg atatagaata
    12781 atcgtccgga tcctcctgta gcaggcaacg gattttcagg atcgttcccg acactaagct
    12841 gtaatctgat tcgatttgtc tccaaaagtt catattctaa ctcttgctgt agatcaggcg
    12901 tctctgcttc ttcgtttgga taatacgaaa tctcaacttt tagtgtaccc gcttcttttc
    12961 ctggaactac ccgatcattt tctatgtcag gttcttctcg ataactaatt tctaaaataa
    13021 agtaacctgg aagttttcta tagctgtcag gtgacttcac ttccttgatc cgatagaccc
    13081 cttccaacaa gtctgaagca ttgaataggg aagaatcgtt tggatctggt gccaagcctc
    13141 cctcgatgat ttcatactct cctgcactat tttgtttctc aattgaaaat tctgcgcctt
    13201 caagacgtcg gctattcata ctgtcaattt tcaaaagttc caaatcgatt ggcgctagtt
    13261 catcttctaa ggttacttgt agtacaccat cgatgatttc atagccttcc ggcaagggag
    13321 cagtttcgtc ttcgatgacc gtatcttcaa tgataaactt accttcttca ttcagtgcaa
    13381 actggaacac ttgatcagat agccgatacc catttggtgc gacagattcc gagacctcat
    13441 attcaccaat agacagatgc agaggttggt cgtaatttaa atcgacatac ttttctactc
    13501 ctacggcacg agaatagaag tatccgatcc caggcaaacg tttcgattct tctggttcta
    13561 acggattacc agtgtatgct cgatacagcg ggaaggaaga atcccccact ttcctgatat
    13621 cgaaacctgc accatctaat gtatggcctt catggtctaa ttttctaact cggatctccc
    13681 gataatcaaa cgtattgtaa gcagtgaatg ctacccataa acgatcctct tcatctgttg
    13741 tatacgtgag atattgccaa ttttcttcac ctgtttgctt gagttggata gtccctatag
    13801 cattatcgta aagtcgcaag atccatgtgt cattttcatc aggttctttg aaaccttctg
    13861 ggttctggct ctcaatgatt ttgtattctg catagttggt agtttcttca ctatccttat
    13921 aaacaaatga atctggctct ccgattggat gaataacttt ctcaccatcc acgattacat
    13981 atttcccgtc ttcatctact gaatagaacg tgagtctccc tttctcttcc tctacttcgt
    14041 tcacaccaga aagccctttc gctactggat atccgatttc cagtccgtta ccatctgttt
    14101 cataaagttc aaactctgcg ccattgattg gcatattact gccttcaatt agtttattga
    14161 tattcatgtg gattggcttg aattcatttc gcatttccag tttgacatac aactcatcat
    14221 actctgtgcc ttctatgtta ggctcttgta caacagaaag cttgtgatat tctccttcat
    14281 aataggaatt cggatcttta ccttccggtc cgccgactct cgaaccagat gtatattcat
    14341 ctacaagctc gtagtgacct aacatctcat cttcgttggc tgcatatttt ttgatttgga
    14401 attgatacgt ctcatctact aactgataac gtggtggtcc agcgatctct ctgatccaaa
    14461 atatgtcgct gactgctcca gtttcatctc ctaaaatcgg catcttcgtt tcaaagatcg
    14521 ctattccgtc cgtggtagtt ttacttgttt cgattggttc ggcttcccca ctctctggat
    14581 tttggtcata taatgcaaat tcgactccag ccaacggctc gccattttga cccttcttgc
    14641 tgacttggaa attacggcct ttatgttcgt aacgaatgga agaagatggg aaatacatcc
    14701 gtccagtagt gatatcattc tcataatcct ccaggttctg aatataggcc atattgttca
    14761 ctgtgcaaaa atttccgtct tgagctcccg catcaagttt tacttggtac ttgatcctga
    14821 ccatttcccc ttcacctaaa gaaatcggag aaaatttata agcttggttt tcccgtccga
    14881 catacgttgt cggagtcgcc actacttctg gtggggcttg tccatgacgt tcttcatccc
    14941 atatttctgg ctcttggtac ttcatgccgt tttctcctgg atctagatgg taaaactgaa
    15001 tatctgccga tctgccagct ccaaatggtg agacattaaa accagtttca tcataaagtg
    15061 tggcttgctg gatcgttctc ttgaaggtag aagagatatc ctccaaggct gtaacaatgt
    15121 cattcttgtt gttcgcattg taatatcgcg gtgttacgcc atcttcttct aaatcagcaa
    15181 tatttttcaa tacatttcgt ccaatagcat ctagtcgttg tctcccgcta acgtttccat
    15241 cgattcctaa accaatggag aagatacttg gcgggttctc ttgatacttg tttttcagcc
    15301 aatgatgata agcaactgtg ttgatcgcag atgacttttc ttgcgaagta ggactgccat
    15361 tcgtattgtt attagaagaa ccatcaccaa cccagtgagt cgctttatta tctccttctt
    15421 ttacctctcc ataacgatag tgtacattta cttcatcttc tggacggtta aagtcagatg
    15481 cataggtaac tggatgagta aaccctgcat tagaagtatt cccagaaaac tcctcaaaat
    15541 ttctgaacaa aacgtcatta tcttcagcaa tcttatcttc catcacagtc cagttgctcc
    15601 aagctccatt agtgctcgta cgataacttg actgaatcgg tgcataactg aaggtcggtg
    15661 tcccatctcc cacaacgatt aggatttttt ctggatttct attttctccg ccattgtctt
    15721 tatacaatgt ttcatagcca tttttaagcc ctagtgtaag cggagttcct ccgattggtg
    15781 tacgatttaa tgctgtattc tcacttcccg tcaaacgatt gatatcattt gtcaatggaa
    15841 agatgtccga actgatttgc tcatggttat tgatagctgt actattatgg taaaaattca
    15901 ccataccgat tcgaatatcc agactcgggt cagagagcag atctttactc atttcgttta
    15961 ctgcctcaat caacgcagca tttttagttt gactattggt cccagcggtt aattcactca
    16021 tactcgctga cttatctaga acaaaaacga tatcgattgg actgatttcc tttttctcgc
    16081 ccccgatgac atctagatag acattgtaaa gggttgttgc atcttcagga tgtagatcag
    16141 atgctgtgct gcttggcaga acaggttcta cccatttttt gatgtacgcc ccgttataat
    16201 ccaagtaact gttttcaaaa ttccgttcat ttccccaaag ctgaacggtt tgagctggaa
    16261 cgtttctatc ttcttcgcct gcagttgatc cattattagt agcatagtcg taatttcgcc
    16321 agttcgctcc cccgtttgta taatcactat catcgatcaa gttctctccg tcatatggcg
    16381 agcctttgtc tccttgatag cgttcattgc tttgtgtcca atttcctaac acttttgtat
    16441 agtcattcgt tgtaaacttc gggtagcgat gctgttcgtt ttcaacttct ccgctaggat
    16501 agatatattc ataggggtct tgcgtagctg ttggcccaag acgctccaaa ataccgttga
    16561 ataactggcg ttgaatgcgc tggagtgctt gaccgctggg tacgattggt tcgtccagct
    16621 tttccatctc attttcttcg ctacgttgaa tttcagcgga atcttctgta tcagtttcat
    16681 tttcttgtaa ttcttggtta tctattcttt catcaaccac cacttgaaga ggtagttacc
    16741 cttcgttcac atcggggatc ttttctgttt caaaaatcaa tgtgccggtt tctgcttctt
    16801 ttgaataaac ttttccgaca taccattctt gaccatcgat cgtctcgact tttaggtctt
    16861 ctccatcttc atcataggag gatagacctg ttccacgaac gttttttacg gtaccaatcc
    16921 cggcggctgc cgcatcgatt gctaaacgta ttctaccttc attggcactt tccttttttt
    16981 caaaagccag atgccattca agggtttctt ctttttcaat atacttccct tcaatttttg
    17041 catagtcctc atctaagaat acctcatcct cctttgtttc tgttgactca gcagctacat
    17101 ccagggtcgt catatgatag actgactgga aatatggtcc aaatagcaaa ctgacagtta
    17161 ctaactgcaa aagcttcaat tttaaccctt tcacatcctt atactccttt caaaaatatt
    17221 tgtcttcttt cacaacaatc aacaaaacaa tgcccatcta attataattt aa
//
LOCUS       DAJSKS010000053        16820 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJSKS010000053 DAJSKS010000000
VERSION     DAJSKS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.054"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..456)
                     /locus_tag="ON396_001467"
     CDS             complement(<1..456)
                     /locus_tag="ON396_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR3113558.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
     gene            complement(561..1424)
                     /locus_tag="ON396_001468"
     CDS             complement(561..1424)
                     /locus_tag="ON396_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR3113559.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1620..3719)
                     /locus_tag="ON396_001469"
     CDS             complement(1620..3719)
                     /locus_tag="ON396_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCR3113560.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="ON396_001470"
     CDS             complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="ON396_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR3113561.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5072..6913)
                     /locus_tag="ON396_001471"
     CDS             complement(5072..6913)
                     /locus_tag="ON396_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR3113562.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7095..9410)
                     /locus_tag="ON396_001472"
     CDS             complement(7095..9410)
                     /locus_tag="ON396_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR3113563.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9595..10455)
                     /locus_tag="ON396_001473"
     CDS             complement(9595..10455)
                     /locus_tag="ON396_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCR3113564.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLCGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10514..11035)
                     /locus_tag="ON396_001474"
     CDS             complement(10514..11035)
                     /locus_tag="ON396_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HCR3113565.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11313..12074
                     /locus_tag="ON396_001475"
     CDS             11313..12074
                     /locus_tag="ON396_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3113566.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12100..14691
                     /gene="mprF"
                     /locus_tag="ON396_001476"
     CDS             12100..14691
                     /gene="mprF"
                     /locus_tag="ON396_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCR3113567.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="ON396_001477"
     CDS             complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="ON396_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR3113568.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16434..16790
                     /locus_tag="ON396_001478"
     CDS             16434..16790
                     /locus_tag="ON396_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR3113569.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4617 a         3491 c         2946 g         5766 t
ORIGIN      
        1 tttttctttt tcatagcatt cctgacagat tccatggaac gttagccgat gatcttttac
       61 taaaaaatga taacgttctt ctactacttg ctccacttca agtaaaagat cctcttctac
      121 ttcctcgatc gtgccgcagt tcaagcatag taaatgatga tggaaatgtt tggcgccttc
      181 ttttctcaaa tcataacggg ctaatccatc attaaaactt actttatcga caattttcaa
      241 ttctgtcaag atttcaaggg ttcgataaac agtagcaagt ccaatttccg gactcttttt
      301 tttcactaaa aagaagattt cttctgcaga taaatgctct ttctcatttt ccaaaagcac
      361 aagcagagta gcttctcttt gcggtgtcag cttaaacccc gcttcatgta gctgctgttt
      421 tgtttttttc atcgccgaaa tagcattcat gaccatttac tcaaccccaa caatttataa
      481 taattacaaa taaaaaaaga tataacacta tgttaattaa aattattata agctagttaa
      541 gaaaaacatg caagggattc tcaattagat tttttgacta gttttgtcac accgttttct
      601 tgtgtaatca atccttgttt caaaagatta cctaatgcgc gcttaaatgc tcctttgctg
      661 ataccaaaag ctttcatgat ttcttctgga tccgatttat ctgtaaagtt gatttgctga
      721 tctgcagaac gttctaaaat ggttaaaatc atttgtgcat catctgatat agcttcatat
      781 gctcttggct tcaatgaaag attcagtaca ccgtctggtc ggacaccaat cactcgacct
      841 tttatctgct cgcctagacg aggttcatta tatcgttcag aaggatggat aaacccaaga
      901 tagaaatctt ccgttaaaac gtacgtacct gttaatttca agcggtatgc tgttcctatc
      961 acatctttgt ttttcatttc ttctgttcca tatttgctta aagatttgaa tattttttca
     1021 tctgctaaac tgccccaaat acggtctttt gcatcaactc taagggaaat catcaagcga
     1081 tccccttttt taggccatag ctctgtcatt gtcggcaatt catcaagaga tacgacaaag
     1141 tctttatctg gaagaccgat atcgacaaac gcacctaaat cttttcgtgt agcagtaacg
     1201 attccaaaag cgtaatgacc gatacggctt ttaggaattt gtgtagtgat ccgattttca
     1261 tgtttttgat tttggtaacc gaatccttca actgcttcac cgattttgtg ttcaccttct
     1321 gatttgctta agccaaaggt ttgtccgttt ttttggacaa agtagttttt ttcattttca
     1381 tcaataataa tacctgtaaa tatagttgct aataaatcgt tcattttttt ctccctctat
     1441 agtgaaaggt tttcttaata catttctttg ctatcataac ataatgttat ctacttgaat
     1501 agctgagcct tggtttggaa aggtgaaaaa aggagtatga caacagttgt gtcatactcc
     1561 cctttttcga ataaacggtg ttaaacggca ggctcacagc ctctttcatt tgcctaaaat
     1621 taattgctcc catttcctcc gaaccatcca gttttacagt tgatggttac gatgtaaaat
     1681 ggcaaagcac tttctccaat gcctttcatc aatgcagatt ttgttgaagt ggcatataga
     1741 ttataatagc cttccccatt ttcgattctg gctttttcta atctgtaacc tccttcaacg
     1801 atatatccac gttggataca tgtagcaatc actttgcttt ggacacctgg tgcccacgtc
     1861 catgctggat tagatgcatc ggcaatatca cttgctctgg cagatttgat tggctgattc
     1921 gctgagtttg aagaggaagg ggtcgaggat gccgcttctt gctgtgctgc ttgtgcgacc
     1981 gcagcttgtt gagcggcttg agctgcagct gctgcttgag cggcttgttt tgcttgttct
     2041 tcagctgctt ttttcgcttc ttctgctttt ttcttttcct cttcttcttt tttctcttta
     2101 tcagtgagca tcttatctac ttgtttcaat tgttcattca actgagcaga tagatctgta
     2161 tttttcactt tttctactgc ttcttttgcc gtcttgtatt ggtcgcgtgt tgctgaatcc
     2221 actacttttc cgtctttaaa gactatagct gttttttctt tagctgtttg caattcagca
     2281 tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt ttccaaaatc tgtgttatcc
     2341 gtttcgattg cttggatctc tttatctgct ttcaacttga tgtcatctgc tagatgatca
     2401 tcgacgatgg caggtgctat gaaccactca ttcacttttt ggatatctcg gatcttagat
     2461 tggatatctt ggtaagttgt ttctaactct gaatatcctt tttcattctt aagactggat
     2521 aaatcatttt ttagttttga taaatcttga tgaatcatat ctgcacgaat aaattgatgg
     2581 tgatcatctg tataaaaggc agcaagtttc tcatgaagtt cttccatttg ttgctgtctt
     2641 tttgcttctg ctgctacttc ggcttctact ttttgatgat ggtcataata agcccagctt
     2701 cctccaccga gtagaagtac tgctgctaaa acgagataag gtactttttt tgattttttc
     2761 ttttgcgctt tatctggatc tgttttttct aagtttgatt cttgatgtgt tttttcagta
     2821 tctggcacga ttgttattga tttcgcttct gcttcaaaca gcttaggctg gttctcttct
     2881 ttttctattt ttgcttcaag atcagtaccc tctttgctta cagattgatt ttcagcatcg
     2941 gattcactat ttatttcatc gggctctgtt gactttttgt ctgtgttttc gagtatttct
     3001 tttttctctg cttccggcag tttattcgtt ttttcctttt ctaattgttt ttcagtaaat
     3061 acttcagttt tttctgtgat atccgttttt acgtctttta tttttactgt ttctttacct
     3121 gtggcgtttt ctctatcaga gtctttttca gtttcttgtt ccttttctgg ctctactttg
     3181 ttttcctgtg caattttttc actgatcatt ttgagcagct ctttcgcgtg ctcttctttt
     3241 gtatcttcac gatgtgcttt gatataagcg gatagtatcg aattttcttc ctcattatcg
     3301 acgatttctt tcagttcagc tactcttctt ttttgttttg attcaagcgt ttcttcttga
     3361 ttctttcctg attcttccga agtctcatga acagttgctt tcttctgttc atttttctct
     3421 atattagtta cttgatcgtg agcaggtggt tcttcgtctt ctggttctgc tttttcgaat
     3481 aattccatta cagattctag aggtaaatct ttcaattccg accattcgat tggatcgttt
     3541 aattctgtat caggaaagac tgcatcttca gttgatttat cctgcatagc tgcatctgta
     3601 tttgtttctt caattgattc ttttatttgc tgatcttctg cagttatttg atgaccgcag
     3661 tctggacaga tagtattctg ctccattttt ttcgatccgc aatttggaca ttttttcatc
     3721 acttagcgat cggctccttt actaggttta cgctaccatt taatctatca tattctatac
     3781 gtcacgccaa tagcttattt ttaggattta cttaaaaata atgaatgaat ttccttccta
     3841 tagaaaaaaa agcttgagac tgcgtagtct caagcttttt ttcttttaaa ataaaccaaa
     3901 cagttttact gatttctctt ctggtcaata gtccacgctt attctaagaa atcttttaat
     3961 tgttttgaac gagaaggatg gcgcaatttt cttaatgctt tagcctcgat ctgacgaata
     4021 cgttcccgag taacaccaaa tactttccct acttcttcca atgttctcgt ccggccatca
     4081 tctaaaccaa aacgtaagcg aaggacattc tcttcccgat cagtcaatgt atctagtaca
     4141 tcttccaatt gttcttttag taactcataa gctgcgtgtt ctgctggact tgttgcttcc
     4201 tggtcttcta tgaaatcacc taagtgagag tcatcttctt caccgattgg cgtttcaagg
     4261 gaaacaggtt cttgcgcgat tttcaagatt tctcggactt tttctgttgg aagatccatt
     4321 tccgcaccga tttcttctgg cgtaggttct cttccaaggt cttgcagcaa ttgacgttgg
     4381 atacgtatca atttattaat tgtttcaacc atatgaactg gaatacggat cgtacgtgct
     4441 tggtcagcga ttgctcgagt gatggcttga cgaatccacc atgtagcata ggttgaaaac
     4501 ttaaatcctt tgcggtagtc aaatttttca actgccttca tcagtcccat gtttccttct
     4561 tggatcaagt caaggaactg catgcctcgt cctacataac gtttagcgat acttactact
     4621 aaacgaaggt ttgcttctgc cagacgctgt ttcgcttctt ggtcgccttc ttcgattttc
     4681 aatgctaacg ctacttcttc ttccgcagtt aaaagggaga cacggccaat ttctttcaga
     4741 tacatacgaa ccgggtcatt gatttttaca ccggttggcg cagaaagatc ttctgttttt
     4801 gcttgttcag cttttttctc agcactcttc aagctgtgaa ttgatggatc accattttca
     4861 tccaccacac tgatacctgc gtcttcaatc ttttggatca atttgtccat ttcatctgca
     4921 ttcaaagtga atggtgtagc aagctgattg gataaatcat catatacaac ttgtcgtttg
     4981 ggtttgtttt ctttgatgaa agctgctact gctgcttcat acgttttttt atctgttcca
     5041 tttgccataa aagaaggccc cccttgagta gctatttggc ttgttttaac tgtttcgtta
     5101 aattgatgat ttcaatcgtc aattcaagct caagctgttt gtttccagtt cgtctggctt
     5161 cttgctgcat ttgttttttt aatgttattt catcagctaa gccagatttg ctgatcacat
     5221 ctagcaagtc ttggatttct cgtggcgaac tttcttctgc caccggtaaa tattcgattt
     5281 ccaccaccaa ccgccttaaa ttttcatctt gcaaaaagtc taaaaactct gcaaggttga
     5341 attcataatg ttgtgagaca tatgcatcga ataaaaaata aacttctgca tatgcatcat
     5401 gaataaactg aaattctcgt tcttttaaaa tattacggac agaaacttct ttaaatgcac
     5461 gatataatag aatctgctct gccttttcta cttgcgtcaa aggtctcttt ttccgtgcag
     5521 tttcttgtct cgtaacctga gtgtgttgac tttcctgagg ttcttgacga ttttgctgat
     5581 ttgtccttcg aagatcgcgc agttgttttt gtatcgtctc tctgccgatc tgaaactctg
     5641 ttgacagttg agatagatac atatcttgtt cgattggtga aacaactcgg ctaagttcaa
     5701 ctagtaattc ttcgaggtat tcgatcttgt ctttttcatt atctaaattt ttcccttgtt
     5761 ttaaataacg tgatttaaaa gtaaagactg tttcgcgccc atgagccact aattcttgga
     5821 aagatgcttc tccatactta cggagatatt catcaggatc aagcttttct ggaacgacta
     5881 cgacagaaag tgctaatcga ctgttttgcc gaagcaattc aatccctcga ttggttgctt
     5941 caaaacctgc gttgtctccg tcatagcaaa agaccaattc tttcgtcagc ttttccattt
     6001 gttggatctg ttgagctgtt agacttgtcc ccatagaagc taatccagtt ttgataccag
     6061 cctgccatgc agcaatcaca tccataaacc cttcgaacaa caaaatttct ccgtttttcc
     6121 ggatatcgct tcttgcttta tcaagattaa ataaaacttg tcttttattg aacagttctg
     6181 tttctggact attcaagtat ttgggctgat ctttttcctg ccctttttca ggtgcaagcc
     6241 agcgtcctga aaaaccgatc gtctggcctt gagaattacg aagtggaaac atcactcttt
     6301 gataaaaacg atcagacata cggccatcat catgcgtgac aaacaatcca gagcgttcta
     6361 acaattcatt tgaaattgat tcattcttga atacttgctg aagaaaagaa cgctcattag
     6421 gtgcaaaacc tatgttgaat tcttcaatta attctaaaga caacccacga ctttgcaaat
     6481 attctaaagc tggctgtcca gcttttgtat gcaatagcat gtgatgatag atttccgctg
     6541 ctttttcatg agcagcaatc agtttccccg tagcagattc aggattttgg acagctatag
     6601 tacgctgacg ccattgatct tctaacggta tctgttcgaa gtctgccact tttaccactg
     6661 cttctggaaa acttagacct tctagttctt gaagaaaagt aaacacgttt ccgcctttac
     6721 cacaaccaaa gcaatgaaaa atctgcttgt cttctgcaac agaaaaagac ggtgtacgtt
     6781 cttcatgaaa aggacacagc cccaagtaat ttttaccaga ctttttcaac tggacatatt
     6841 gtccaatcac ctcaacgata ttcgtttgac tgcgtactgt ttcgataacc tcttgaggaa
     6901 tccgttgagc cattttttca ccccctgctc tattgatatc ttttgggttt aacagaagat
     6961 atttcaggag aataaccggt aaaaaatcgc actaactgaa atcagtgcga ttttaaagga
     7021 cacaattata catgtttgat tgtatcatat ttcattgctt tttcaagtat aacacatttc
     7081 tatctcatta agcatcaatt tgctactctt ttcagtaact gattttgaat atcagccttc
     7141 cagtacaata ctcgaagaac aatatttacg agcatgaata caaagaagta tcctacgctc
     7201 tctcccaacc ctaagtttac ttccaaaatt ccatggaaca gcattgcagc tacatatatc
     7261 cctacgatcg tcgttactgc taggaagatg cgcaatgggt tgaagggata acaagctttt
     7321 acaactgcca tgcaactgat cccaatcagc aagtaataca tcaaagtagt agtttcagca
     7381 tttccccatg cgaatgtttt tccaagtaga tacaccgcaa tgatattcac tactacaagc
     7441 aaggcatttg gtaaagcatt aataagtgct gtaggcaaat aacggtaagt gatttttctt
     7501 ttatcttgtt caaaagataa aaagaaggaa ggataccctt caatcgctaa atcaatcagc
     7561 gtgatttgga tagggataaa cggaaatcca attgctgtaa cggcacatat aatcgataaa
     7621 ataaatgaat aaatcgtttt gataaaaaag atgcctgaga ctttcgtcac attgttgacc
     7681 actcggcgtc cttcaaacag gacttctggc aatgtagtaa aatcagagtc caacaatacc
     7741 agattcgcaa tctgacgtgt tgctccatca ccttctgcca tagcaatgct gcaatctgct
     7801 tctcttaacg ccaaaacatc attcacaccg tcgcctgtca ttgcaaccgt tctgccgctt
     7861 tctttcaatt catttaccag caatttcttt tgctgcggac tcactcgtcc aaaaaccgta
     7921 tagcgatgag cagcttctcg cacttccatt tcttcagtaa tttgagaaag atcgacatac
     7981 gcatcataat tcggtaatcc tgcacgtcta gcgatattgg agacagtaaa aggattgtct
     8041 ccagaaatca ctttcagatc cacaccttcc tcatgaagat aagctagtgt ttcttgagcg
     8101 ttttttcgga ttggatcatc gatttcaaac agagcaaccg gctcaacaaa tgctggtttt
     8161 tcttcgttca attgctgatt tggagcgatc cccaacataa gtacgcgata gccattcgct
     8221 tgggctgttt ttagactgtc cggaagccga tcttcttcca ccaaacgttc aggcgctcct
     8281 acgtacacga ctcccagttc tggaaattcc atcgcgcccc atttgcgatc agaagaaaat
     8341 gcgactttgt ttgtcacatc ataccgatta gaaagctcaa aataatctct catagcccgc
     8401 atagtgatgt tgttatcact gctttctgcc agatacgtcc ccataatttc tgggatggct
     8461 ttctcgtaca gcggatgtag tgcttctacc cgctggatac tcattttccc ttcagtaatg
     8521 gtccctgttt tatccaagca taatgtatcg acatgggcca gcgtttcaat tgaatacatg
     8581 tcttgaacaa ggattcgttt ttttgccagt ttggttaccc ctgtcgttaa tgcaatactt
     8641 attagcaata ccaaaccctt tggaagcatg cctaacaaag ctgctgcaga tgcaataaca
     8701 gaaattttga tagaagcatc gcggatgaac agtgcttcca aaaatagtat gatccccaaa
     8761 ggaataatca cgaaactagt gaatttggat actttgcgaa tcgaatctac aagttcagaa
     8821 cgaatagctt tgtgagtctt tgcctcgcta gtcaagcgga cagcataatt atctgcacct
     8881 actcggataa cttcagccat gacagaaccg cttgtcaaaa agcttccaga aagcaattca
     8941 gctccagttt ctttgacaat caaatcagat tcgcctgtca aaagagcttc atttgcttct
     9001 gcccgtccag atataacctg taaatcggct ggtatctgtt ctcctgctcc gatttttacc
     9061 aaatctccta ataccagctc tttagtagaa atcgattgtt ctttaccgtc ccggatcact
     9121 agtacttctg attgggaaac aatcgataat ttccgtacaa gatttcttgc atgtatttct
     9181 tgataaattc caataacgat attgacgaca ataattgcta aatacgccat gttggaatac
     9241 gcacctacaa ataacagaca aatcccaata ccaaagttca gaaaattgaa taacgtcaat
     9301 atattatcgc tgaatatttg ttttgtactt tttgatgcat cttcctggta gtcattttgc
     9361 tggccagaag ccattttctg aactacctct tgctgtgtaa gtccattcat tttctttttc
     9421 tccattttcc gccaactttc aaaaataatc acgtgtttaa gtctagcgtg ttccaagatt
     9481 aaaaacaact tgtttttata aaatttatag aaaaacacac gaatgatttt acaaaaaaga
     9541 tgccaacctt tttgtttgaa tcacccgtgt gctgttttta ttatcaatct atctttactt
     9601 atattgttcc tccaaacggc tcatccacca acctagtgct gtaccggcta taaaaagaac
     9661 aggacaagct agtaccccag caactcccaa tcccgcgtaa tcggtaggaa tgatcataat
     9721 cgttgaagca aaaactacac ctaaaatgaa atggaatagc ttgctatatg cctttgagaa
     9781 aatgtaatcc atgatttttg acaatccaag aacacaaaga atcccaccaa ttccgatcgg
     9841 cacaatcact tcaagtcgta cttctttaaa tccgtcagcc atcgctttat acaatcccat
     9901 atacaccaaa aaattagaag ggctcaatcc tgggacgatc atacccaaac caatcaatcc
     9961 gccggcaatc agccatgttc caaaattctg atcgacttgc ccgtcaaata attgagcgcc
    10021 gcataataaa aacaaataag ccagaacaaa agtaacaacc atgatgatca catcagaagt
    10081 atctcttcct tttttccctg cttctttcca taacgcagga acagttccag cgatacatcc
    10141 gacaaaaaac cataatatcg ttgtttcata cgcacctaat aaaaaactga ctaaaaaaga
    10201 aagtagaaag atgcctgtca ctccgcctag accaacaggc agaaaaaaca aaacattttc
    10261 tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc cgctcataca tgccaaagac
    10321 agctgctaat gcaccaccgc tgactcctgg caagataaat cctgaaccga taaacattcc
    10381 cttcacaaac cgtaacatcc agtctttggt tgtgtacccc acttcttgat tttccatttt
    10441 ttgccttttt tccactattt gcgcactcct tctttcattt aattttattc atgattgaac
    10501 atttcctgct attttactta aagacttttt cataggtctc tattattttt tcattcattt
    10561 tgacaatatc aggataaaac atgttacctc cattatgata gagatagccg ccattgtaaa
    10621 gcaaggactg gatccgccaa tatcggtatt gtttcccttc ctcatttcct aaaagtgggc
    10681 tcagcacatc tttagaatac gcttctgcta attctactgt gttttctccg ccatgatttt
    10741 ttacataggc aatataatct ttcccaaaat tatatgcttg gacagctgtc cataaatcgc
    10801 atccttgttt ctttgcttct actatcattt ccgccaaata tttgacacct tgatcaatac
    10861 tgtcagctgg attatcaatg atctcagctt ctccatgcag actttccgag ctctgcatga
    10921 gatctgtccc ttttcctttt gattcggtca atattatcgc cttgatcaaa ttctcgtagt
    10981 tttccatatg ataatttttt gcagctgttg ttatttctat ttgatagctt tccactcgtt
    11041 ttaccgaacg ataagttctt cctcataaaa acaacaccgc accaataacc gtcagaatga
    11101 tcatgtaaaa aagaatacgg ataatagata atcgttttcg cttcatttca tcacctagtt
    11161 attttgacat ctttttttat cttactattt ctttatcttc ttggctattt cttccttgat
    11221 ttctgtgtac gccttaagaa aaaatgaatt ttcctgtatt ttttgacatc ttacgaaaat
    11281 gatcgttgtt tttcagtaaa aacaattgcc caatgactga tataatcgtt atactttaca
    11341 cagtaggtga ttatgtgaaa aaagaaaaga aatatgcaaa aatgccagat gggagcgaaa
    11401 tttactatga aaagagcggt caaggcttcc ctctcttttt actgcatggc aatgacggca
    11461 gcggccgctt tttttcagaa caggtccctg tactcgaacg ctactatacc gtgtatctag
    11521 tagatagccg aggacatggt cgttcgacaa acgaagcatc tatgttgaat tttcagttaa
    11581 tggccgagga tctgaatacg atcatgttgc tggaaaaaat cgatcaggca gattttcttg
    11641 gatttagtga tggagcaaat ctagcactgg tatttgccag cagcttccct aaaaaagttc
    11701 atcgattgat tttaaattct ggaaacacgc ttgttaaagg cgttcgtttt tctgccagag
    11761 tcatcagtaa tattcattat gcatgggtat ggttactctc tttatttcgc ccaagtcttc
    11821 gcaaaaatct tttagttatc aaacttttgc tgcatgacat cgggcttaca gaaaatgatt
    11881 taaaaaaaat caactccccc actttgatca tcgtgggaaa aaaagatgta atcaagctaa
    11941 aacattcttt gtatatagct aaaactatac caaaagcttc ctttgtgctt gtgaaagaac
    12001 aaggacatga attagctaga aaagatcctg aacgcttcaa tcgagaagtt cttcaatttc
    12061 tttctgaaac ataactatta ggaatagaag gtgatttatt tgttaaaaaa taccatacaa
    12121 tggctgaaaa accacctagg actgttcaaa acaatctttt tgatttctgt cattgtcatt
    12181 attgtcggtg aattgatgtc catcggtaaa actctatcta tccagcaatt aggagaaacg
    12241 tttgccacta tacctgtatg gaaaagcgga ctaatgctat tgattggtct tatctctgtg
    12301 ttacctatgg ttggttatga tattattttg aatcggatgc ttgaacaaaa gcagaatcct
    12361 cgttacctgt ttgagacaag ctggctgatc aatacgatca acaatattgc cggtttcggc
    12421 gggtttgtta gtgtggggct tcgctctgaa ctatatggac aaaagaaaga gagcagaaac
    12481 gtgatccaag cattatcgaa agttttcttg tttttgatgg ctggactttc tatttacagt
    12541 ctgctttctt ttttacttgt agtcttcacg ccagttgctc cttttttgaa gcaatattgg
    12601 atatggttga tcggcggcgg tctatatttt cctgtcgtct ttttatttac tactttcaaa
    12661 aagaaaggat tccttggtgg tacctcactt aaaactcaag gacagctatt gctagtttca
    12721 ttgttggaat ggaccggtgt cctgtttagt ttcctttctg ttggttattt aatggaaatc
    12781 cggattgatc tgtggcaaac aattccatta ttcattgctg cttcagtgat aggaatcgtg
    12841 tctatgattc caggagaaat tgggagtttc gatgtaatga tgattatcgg gttgtccgct
    12901 attggtgtgc caagagaaat agtcgttgtg tggatcttgc tctatcgcct gttctactac
    12961 atcattccgt ttttgattgg aatcgtcttt ttcttcaaaa atatcggttc cacttttgat
    13021 cagcgttatt ctggtatccc taaacaatta gcaacagaaa ttgcgcataa aatcgtggtt
    13081 gtcttattat acttttctgg aatcatgcta gtactttctg ccactatccc gcaagctttt
    13141 acagagtttc gctggcttca tagtttgaac ccattgaaat ttcattttat tatccaattt
    13201 cctagtatac tattgggatt tcttttaatc attatgggca gagggatcgc agcacgtgtg
    13261 aaacgagcat atttgcctac aatcttcttg attgttctag ctttacttta tgttttatta
    13321 agtgatttta gtttcacacc ggtggttttc ttgtctattc tgcttctgat cattcttgct
    13381 tctaaaaacg aattatttcg ggaacaattg atctattcat gggagtggcg tacgatcgat
    13441 ggaatcatta ttggtgcact gtccttgctt tacatcgtta ttggggtata caatctgccg
    13501 gatttccctc atcggcgtca ccattttatt tcctttttct tatttccttc tgaaaagatt
    13561 tggttttcgg gattgttggc catcatcgct gttagtttca tgatcgttct atttgttcat
    13621 ttccttcaag gagaaaagaa acagattggc gaagccttta atgaagaaaa ggccctgaag
    13681 atccttacaa cttatggagg aaactccgat agtcaattga tcttcttaaa agataagcgg
    13741 atgtttgcct atgaaaaaga cggtgaacca actgttcttc tccaattcgc ttgcttcaat
    13801 aataaatgta ttgtaatggg tgatccttct ggtaagaaag aagattttcc agaagcaatc
    13861 gaagctttta tcgaagaaac agatcgacta tgctatttac cagttttcta cgaaacaagc
    13921 gaagagattg ttatgatttt gcatgaattc ggctacgatt tcattaagat gggggaagaa
    13981 gcttatgtag acctgaatag ttttacaact tcagggaaaa aaatgaaggg cactcgtgct
    14041 gtcttgaatc ggatcgaacg agaaggattt acatttgatg tcttacaacc accattttct
    14101 actgagcaaa tgagtacttt caaaaatatc tctgataatt ggttgggttc acgaaaggaa
    14161 aaagggtttt ctttaggctt tttctctgaa gattatctac aaagagcgcc tattgcagtt
    14221 gtcaaaaata cccaagaaga aattgtcgct tttgcaacga tcatgcctac ttacacgaac
    14281 aataaagtag gaacgattga tctgatgcga tatgattcta aaaaagcacc ttctggcagt
    14341 atggatttct tgttcttgca tttgtttgcc tatatgaaag aagaacatat ccaatggttc
    14401 aatttaggga tggcccctct ttcaaatgtc ggaacttcca gaaagagttt tttacaggaa
    14461 aggattgcat acttggttta cgagtttggt tctcattttt attctttcca tggattaaga
    14521 gaatataaaa gcaagtacgc tacaaactgg gtctcacgtt atactctata ttctagagat
    14581 agttggattg catatgttat gattgctttg ctgattattg ataatgcgcc agttgagcag
    14641 acacaaggta gaataagtgg tttgaaaaaa tggatacgaa gaaagtatta aaaactgcat
    14701 accgatagat gaacactctc atgatgaata aaagagactg aagtaaaacg aaagttcatg
    14761 cttctacaat ctcttcttat acaattgaag aacaaagagc ggtccaaaca gaaatcctgt
    14821 ttggaccgct cttttatttt aaacattttt cattaccaaa atattcgagt cgcgtttctt
    14881 atgcttcgat ctctgtaacg atacctgaac ctactgtccg tccgccttca cgaattgaga
    14941 atgtcgttcc gttttcaaca gcgattggat ggatcaagtc aacatcaatc gtcacgttgt
    15001 caccaggcat gaccatttct gtatcttctg gcaatgtgat tgttcctgta acatcagttg
    15061 tgcggaagta gaactgtgga cgatagttgt tgaagaatgg agtatgacgt ccaccttctt
    15121 cttttgtcaa cacgtacact tctgctttga attttgtatg tggagtaatg gaacctggtt
    15181 tagcaagtac ttgtccacgt tcgatatcgt cacgttggat cccccgtaac aacacgccta
    15241 cgttatcccc agcttccccg taatctaacg ttttacggaa catttctaca cctgttacaa
    15301 ctgctttttg tgtttcaggt ttgatcccta cgatctctac ctcatcaccg acacgaacag
    15361 caccacgatc aatacgacct gaggcaacag ttcctcgacc tgtaattgag aagacgtctt
    15421 ccactggcaa tagtaatggt ttatctgtat cacgttctgg tgttgggata tattcatcta
    15481 ctgtatccat caattccata atagcagctt cagcatctgg atcgccttgc aatgctttta
    15541 atgcggaacc tttgatcaca ggagtgtcat cgcctgggaa accatattcg ctcaataact
    15601 cgcgaacttc catttctacc aaatcgatca attcttcatc atcgactaaa tcaactttgt
    15661 tcaagaaaac aatcaggtat tttacaccaa cttggcgtga caataaaatg tgttcacgtg
    15721 tttgaggcat cggaccgtca gttgcagaaa caactaagat tgcaccatcc atttgtgctg
    15781 cccctgtaat catgtttttc acatagtccg cgtgtcctgg tgcatcaata tgcgcgtagt
    15841 ggcgtttttc tgtttcatat tctacgtgtg cggtattgat cgtaatccca cgttcacgtt
    15901 cttctggagc tgcatcaata ctagcatagt cttgaggatt tgctaggcct tttttgccta
    15961 atactgtagt gatcgcagca gttaaagtag ttttcccatg gtcaacgtgt ccaatcgtac
    16021 caatgttaac gtgtggttta cttctgtcat aatgttcttt tgccataatt tacaaacctc
    16081 ctaaataata tagatcactg aaaaatttca gctctcacct ttttattata gtcttttttt
    16141 gactaaatca aaaagaatac gtccgactta ctttttgtaa gattcgaact aagcttatta
    16201 tacgtcaata ttggtcaaag tcaactaaga gcactcgtct gtcttatacg cgaaaaaagt
    16261 accttttata aataaaaaaa tgtgtttttt gagaaaaact tcaagatagt ccttaacttt
    16321 cttttgaagc aataagaaca aaatgaggaa aagtttaaaa ttcagctgtt gccaacaact
    16381 ttcaatagac gaaatcttct atttcgttaa taataaggaa agaggtgaag tacatgaaaa
    16441 aattaattgg cgtacttgtc gttttaggca tatttctcgg cggaagtttt gcagcttatc
    16501 attattttta cggcggcgaa gcttattata caaaaatcac gacgaatggc gaaatgtcag
    16561 ctacccaagc agataacgga gagaaattca cgacttatac ctacaagcaa gcggcttata
    16621 ataaagatgg tgaaaaaaaa caggtgacgc ttcgcgaaga aagagaacat ccattaaaga
    16681 tgaatgcgta tcttaaactc aaagtcaatc ctcgtaaagg tgtattaagc tgggaagaaa
    16741 taaaggcttc tgaagtgcct aaaaatgcgg cagaaaaaat cgataattag taaatggtct
    16801 taaaatttta ttagataaaa
//
LOCUS       DAJSKS010000054        16697 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJSKS010000054 DAJSKS010000000
VERSION     DAJSKS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.055"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            86..1786
                     /locus_tag="ON396_001479"
     CDS             86..1786
                     /locus_tag="ON396_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR3113570.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            1756..1974
                     /locus_tag="ON396_001480"
     CDS             1756..1974
                     /locus_tag="ON396_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HCR3113571.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            1994..2884
                     /locus_tag="ON396_001481"
     CDS             1994..2884
                     /locus_tag="ON396_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR3113572.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            2902..3186
                     /locus_tag="ON396_001482"
     CDS             2902..3186
                     /locus_tag="ON396_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113573.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            3673..4287
                     /gene="gmk"
                     /locus_tag="ON396_001483"
     CDS             3673..4287
                     /gene="gmk"
                     /locus_tag="ON396_001483"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR3113574.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            4292..4597
                     /locus_tag="ON396_001484"
     CDS             4292..4597
                     /locus_tag="ON396_001484"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR3113575.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            4850..7258
                     /gene="priA"
                     /locus_tag="ON396_001485"
     CDS             4850..7258
                     /gene="priA"
                     /locus_tag="ON396_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR3113576.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            7273..7764
                     /gene="def"
                     /locus_tag="ON396_001486"
     CDS             7273..7764
                     /gene="def"
                     /locus_tag="ON396_001486"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR3113577.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            7757..8695
                     /locus_tag="ON396_001487"
     CDS             7757..8695
                     /locus_tag="ON396_001487"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR3113578.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            8695..10053
                     /gene="rsmB"
                     /locus_tag="ON396_001488"
     CDS             8695..10053
                     /gene="rsmB"
                     /locus_tag="ON396_001488"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR3113579.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            10066..10806
                     /locus_tag="ON396_001489"
     CDS             10066..10806
                     /locus_tag="ON396_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HCR3113580.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            10803..12872
                     /gene="pknB"
                     /locus_tag="ON396_001490"
     CDS             10803..12872
                     /gene="pknB"
                     /locus_tag="ON396_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HCR3113581.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            13037..13936
                     /gene="rsgA"
                     /locus_tag="ON396_001491"
     CDS             13037..13936
                     /gene="rsgA"
                     /locus_tag="ON396_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCR3113582.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            13949..14599
                     /locus_tag="ON396_001492"
     CDS             13949..14599
                     /locus_tag="ON396_001492"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR3113583.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            14600..15238
                     /locus_tag="ON396_001493"
     CDS             14600..15238
                     /locus_tag="ON396_001493"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCR3113584.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            15393..16247
                     /gene="mreC"
                     /locus_tag="ON396_001494"
     CDS             15393..16247
                     /gene="mreC"
                     /locus_tag="ON396_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR3113585.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            16251..>16697
                     /gene="mreD"
                     /locus_tag="ON396_001495"
     CDS             16251..>16697
                     /gene="mreD"
                     /locus_tag="ON396_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR3113586.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLL"
BASE COUNT         5602 a         3021 c         3669 g         4405 t
ORIGIN      
        1 tttcttatgg aatctacaaa gtcgaatacg agactcatga cctttttatg acgtactggt
       61 atacttgaag aaaaaggaga gcgcgatgga gataaaaaaa gtttactttt cacagaaaat
      121 gtcgctgtat tcgcctatcc agcaagcaaa acaacccctt ccattttttc aaagtcatca
      181 agaaagtaga gacgcttttt ggcgttctgc aaaaaagcaa tgtgctttta aagcagtatc
      241 gaaaaaatat accgtctatt ttactattgc taaaagtaga ggagatgtta tttttaataa
      301 tctgttgatt gaaggtgaaa cttttgaatg gaaaaaattt tttcggtata tggaagcttg
      361 cgctcgtttt tttattaaag aaaactgttg ttttttattt caatctcctc tatcagaaga
      421 atggttgcgt atttttcata aaaacggcta tcaaggaacc acacaattaa ataaaaagct
      481 ggtctatcat acagcacttg ttttaggtgg aggaggagcg cacggtgctt atcagattgg
      541 tgtgtggcag gcgctgaagg aacatgatat caactttgag attatcacag gtacctcggt
      601 aggtgcgttg aacggcgcat tgattttaca aggagatatg aaaaaagctg tgaatttatg
      661 gaaaaagctg tcgacaagac aagtgcttgc gttaccagaa atggctgcag ttgaagacct
      721 tcgtgaacgc ttctatcgag aaagacgcca aatgacgaaa acagcactaa ttgaaggcgg
      781 ggtttcttct gctccattgg aaaagatggt caaagacaat ttggatttgt ccttactcaa
      841 acataatccc aaaatacgtt tatatacggt gtctactaaa ctgccagaac tagcggaagt
      901 agtgactgat atccaacaga caaaaacaga agaaatacca gattggatac tcgcctctgc
      961 ttcattttat cccgctatgg cttatcgaaa gatcggaaaa aataaatatg cagacggtgg
     1021 gtatcgcaac aaaataccta ttgatattgc gattaacaaa ggagcaacag aggcatttgt
     1081 cgtagatgtg caagggccag gcccagcgaa aaaaattcgt atgccagaca tttttatcca
     1141 ttggaaatgt caaacattgt ggacactagg cagctttctg ctttttgata gccaaaggaa
     1201 ccaattaaat ttacaattag gctatctgga aatgaaaaaa cgattaggct attactatgg
     1261 gaactggtat acatttcatt ctgttaaacc tgcaagaaca tactggcgag gatttttgtc
     1321 ttacttaaca aaagaaattc agttagaatt atcttttttc aaaagcataa aattttggca
     1381 aaaactgcgt aatttatata agaacagggt cgtaccagag acatgcggat tggcgatgct
     1441 agaactttta gcaaaaaaac attttttact gcccaatgaa atctatcaag tagacatgat
     1501 gattcaaatg atctgccaac aagatataaa aagtatgccg gatgatttat tggcacaaat
     1561 tggtcaatta agtactgaag aatggcgaag gtatcgaaaa taccagcaaa aaatacaaaa
     1621 tgagcgcacg aaaactgttt attttgatta tctgctacaa ggaaaacgca aggatcgttt
     1681 gcagcatgaa ttgcttaagc aaccagttga cacattactg atcctatatt tatattatct
     1741 caaggaggaa caaccatggc acaagaattt tcatatgaaa tcgtagaaga aatcgctata
     1801 ttgtcagaaa acaacaaggg gtggcgtaaa gaactgaacc tagtcagctg gaatggtcgc
     1861 ccgccaaaat tcgatcttcg agattgggcg ccagaccatg aaaaaatggg gaaaggcctg
     1921 acattgacga acgaagagtt tgaacaactg caaaaagcaa tagaaaatat gtaatgagga
     1981 aggaccctgt cgaatgaaaa gtatgactgg ttttggcaaa gccatccgcg aaacaaaagc
     2041 gtatcagtta gatattgaaa taaaaagtgt caaccagcgt tttctagatg ttcaaatacg
     2101 aagtcctaaa atcctgaact ttgttgaaaa cgattttcgt cagcttatta aacagcagtt
     2161 aagccgcggt cgaatcgaag tatttgtgaa tctagcttat attggcaaaa acgaaaaaca
     2221 gatcgtcatc aactgggaat tgattgattc acttatcact caattgacag acggaattca
     2281 acaacgatac ggtagtcaaa atcaagtgtc aattagtcgg atggcggaac gcttcgtgct
     2341 ggacgaaaac ttcgtaacgg tagaagaaaa accggaagag agcatggaag aattggcaca
     2401 gttagctcta gatacattga aaagcgcact tgattctatt gaggcgagtc gagaaaaaga
     2461 aggtcaagct ttagctttag tgttgaaaaa gaatactgaa gaattcaaag aagtactaaa
     2521 aaaactcgat agttttgtag aaatctatga aaaggaattt caaataaggt atcaaaaaaa
     2581 gctggaggaa tttcttggag cgaaagtcga tgagcaacgg ttgttgacgg agctagccat
     2641 cttgttggaa cgcggagata ttcacgagga actggatcgt ctggcgatcc atgtacaaaa
     2701 actagatgaa cttatcgaag cacaatgtcc tgtaggtcgg gaattagatt tcctgatcca
     2761 agaaatgaat cgggaaatca acacgatcgg ttccaaatca aatgcaatcg aaattaaaaa
     2821 tcaagtcgtc cagtctaaga cgatcttaga aaaaatcaga gaacaagtac aaaatattga
     2881 atgacgaaag aggtagagat aatgaaatat tatgacgtaa cgttccacga attaagtgga
     2941 aaaactgtgg ttaaacggga tatcccttct gaaaaaaacg gctttgacgt atggaaagat
     3001 gcctgtgcag actataatga gaacgaactg tttattttga tcaacgacgg tgcttacgtg
     3061 acgatgaatc ggaaatttat tgttcgaatt gacacagaag aggtagaaga tccgacagaa
     3121 aaagcacgtt ctcgaaaaga tgagatcatg ggtgtagtga atactttgtc caatatgggc
     3181 ttttagcaag gatgctgaac aaagaaatac aataaataaa cagaattgga tttcttcgct
     3241 tagcttcttt gctctaggaa tacatttaaa ggttgtgaca aaattcgtgt cacaatcttt
     3301 ttttattttg aataagtgat gttcaaaaac cattttctcg gtattaagcc aaatttatgc
     3361 aaaaatgtga gaaactattt tcacaaaatt tttcttcata ctcgaaacta atcggcttct
     3421 tcacgacctc ttatacacgg cgttcaaaag cttgcatctg cgataataac cccaaccaaa
     3481 cgaaaaacat gagaagctgt ttatgtttgg ctgttcttat tacttgatgc aggacagctt
     3541 ttttacgacc tcttggtaca gggtagcttt tttaccaatt tctagctata attaaagata
     3601 accttgaaag ttgcagaaaa aaagtgcaaa ataaacgata agtacaaaaa gactagttga
     3661 aaggacgtcg tcatgtcaga gcgtggatta ttaatcgtgt tgtctggtcc atccggtgta
     3721 ggaaaaggca cagtacgaaa agcaattttt gagagtgagg ataatgattt tcagtattcg
     3781 atctcaatga ctacccgtaa aatgcgtgaa ggggaggtcg aaggcgttga ttatttcttc
     3841 cgaagcaaag aagagtttga atcaatgatc gaagctggag agatgctgga atacgcagaa
     3901 tatgtcggga attattatgg aactcctttg tcatatgtca acaagacatt ggatgaagga
     3961 aaagatgttt tcttggagat tgaagtccaa ggagcaaaac aagtcaaaga aaaagtgccc
     4021 gatggtgtat tcatcttttt gactccgcca gatttagcgg aattaaaatc acggatcgta
     4081 ggccgaggaa cagatgcaga tgaagtaatc gaaaaacgaa tgaaagttgc aaaagaagaa
     4141 atcgaaatga tggctcttta cgactatgct gttgtaaatg atgaagtgcc gctagcagtt
     4201 gaacgaatca aaaacattat tgccagtgaa cattttcgag tagatcgcgt aatcggtaaa
     4261 tatattaaaa tgttaaagga gatgtagtct tatgatgtta aaaccttcaa ttgactcact
     4321 attagatcgt gtaaattcaa aatattctct agtgatttta gcaagtaaac gcgcgcatga
     4381 attggatgca agtgcacagc ctactctaga ttcttttgat tcagtaaaaa gcgtcggtca
     4441 agctttagaa gaaatcgatg caggaaatgt agtcaatgat ccacatcctg aattaaaacg
     4501 tgaacgtttg aagatggaag aagaggaacg tcaagcacaa aaagagcgtg aacaacacga
     4561 attggaatca cgtatccgtg aagagcaaaa aatgtaataa agaaaaatag agcgaacatc
     4621 taaaaagaaa agcaaaccca gctgtttcct tttcaaacat tcgccactta ttgtctggta
     4681 gaaaaggctg tgtctgaact ttttgacaca gttctttttt ttcttatctg ctgttaattt
     4741 tttgttagga tttggcagaa gataagacaa atccatcgat ctctagtaaa ataggcaagg
     4801 aactttttgt cgcttgataa gaaaagacaa aaaaaggagg aaacgaacca tgacattaac
     4861 agctgaagtc attgtggacg tacctactat gcagaccgat cagccattca cttatttggt
     4921 tccctctgaa atggaaacag ctttgcaagt cgggatgcga gtagaagtcc cttttggtaa
     4981 tggcaatcgt catgtccaag gatttgttat ggctatccaa aaatcagaag aatcagctaa
     5041 tccatctttg aaggcagtta ttcgtttgct agaccttgct ccggtggtca atgaagaatt
     5101 actttcatta gctgattata tgaaaaaaat tacttacgca ttcaaaatca cttgtctaca
     5161 aacgatgctt ccgagtgtga tgaaagcaga atatgataaa ttgatctatc ctttggcaga
     5221 cacaccagaa gttcaggtat tgtttgatca gcgagaagtg atttcctgga aggaagcaga
     5281 agaagaaggc agcctttctc aattgattcg ctggcgccaa gagcaattag tagatatcaa
     5341 atatgaagtc catacacgaa ataaagtaaa aacgattcgt ttggtccgtt ctttgttaac
     5401 tgaaaagcaa atcgaagaag aatgggcaaa attacggcaa aatgccaaga aacaaaaaga
     5461 acttcttttg tgtttatctg aactgagcca agaagaacca attgcttatt tcaaaaataa
     5521 aggaatcagt actgctgtct tgaatcaagg aaaagaaaag ggctggctgg agtttataga
     5581 atcagaacgt tatcgcgacc cttataaaga ccgagtattt gaccaaacga cggcattgga
     5641 actgaatgca gaacaaaaaa atgctgtcga acaaatcatc acagcaggtc agcagcaaaa
     5701 agatcaggtc tttttgctgg agggaatcac tggcagcggg aaaacagaag tatatcttca
     5761 ggcaatcgct gatgttttgt cagaaaataa aacggctatt atgttagtgc ctgaaatcgc
     5821 attgactccg caaatggtcg agcgattcaa aggacgcttc ggagaatcag ttgcggtctt
     5881 acatagcgga ctttcccaag gtgaaaaata tgatgaatgg cgcaagattg agcgggaaga
     5941 agcgcaagta gtagtaggcg cacgatctgc tatttttgcc ccgctgaaaa atatcgggct
     6001 gatcattatc gatgaagaac atgaatcaag ttacaaacaa gatgagacac cgcgttatca
     6061 tgcgagagat ctagcaattt ggcgcagtaa atatcatcat tgcccaatag ttctaggaag
     6121 cgcgacgccc tctttagaat caagagcacg agctcaaaaa ggcgtttatc agttattgca
     6181 gttaaaccac cgagccaaag cagctgcaca gcttccagct attgagatcg tagatatgcg
     6241 tgaagagttt cagaatcatc ggacctctac tttttctgct aatttgcagg aaaaaataca
     6301 aaatcgatta gacaaaaaag aacaaactgt cctactgctc aatcgtcgag gatattcgtc
     6361 ttttgtgatg tgtagggact gtggatttgt tcttccatgt cctaattgcg atatctctct
     6421 tacactgcat atggatacac gttcaatgcg ctgccattat tgcggacatg aagaacctat
     6481 tcccaatcgc tgtcctaatt gtggcggcaa taaaatccgc tactatggaa caggtactca
     6541 aaaagtagaa gaggaactcc gagaacttta tccgcaagct aggattttgc gtatggatgt
     6601 agataccact cgaagaaaag gggcacacga acagatttta caaaaattcg gtgcaaaaga
     6661 agcagatata ttattaggga cacaaatgat tgctaaaggt ttggactttc cagaagtgac
     6721 attagttggg gtgttgaatg ctgatacctc cctgaattta ccggattttc gttctagtga
     6781 gcatacattc caattgctta cccaagtttc aggacgagca gggcgagcgg aaaaagcagg
     6841 cgaagtcgtg atccaaacat tcaatcctca acattacgcc atagaattgg ctaaaaaaca
     6901 aaattatgag cagttttatc agcaggaaat gcatgtacgg catcgtggag gctatcctcc
     6961 ttattacttt actgtgaaaa taaccgccag ccatcctgaa gaacaagtag cggcaaagaa
     7021 aatctttcag attgccaatc agctaaaaga agtgttaagc cctcaaagct tgctattagg
     7081 gccaacgccc agcatgattt tgagagtgaa aaaccgatat tattatcaac taatcatcaa
     7141 atataaacat gaaccgaatc ttcctcatgt tttggatgaa attttgaatg gaagccagaa
     7201 agaacagcga caaggtcttt ttgtggcaat tgacaacgaa ccattaaact ttatttaaga
     7261 aagggccaaa ttatgcgcta tcctatattg atccacccta atgataaatt aaaaagaact
     7321 gctcagccaa tcgatgtaat cacggacgag acaatcgctt tattagacaa tttatatgag
     7381 acaatgattg cgaatgacgg aatagggatc gctgctcctc aagtaggaca aaacaagaga
     7441 attgctgtga tcgaagtaga tgaaggcgag aaatttgaac tgattaaccc agaaatcatc
     7501 gaagcaaaag gtgaaagcct tgacgtagaa ggttgtctca gtattccgca tgtctatgga
     7561 acggtcaagc gagcagatga agtgaccgtg cgttattatg atcgagacgg agaagagata
     7621 gaagtgacgg cttttggcta tttagcccgt gccttccagc atgaaatcga tcacttagac
     7681 ggtatcttgt tcattgagaa aatgatacag cagatcccgg aagaagaact tgaagaatat
     7741 atggaggaac atcttgatga ctaagctagt atttatgggc acacctgctt tttctgtgcc
     7801 gattcttgaa gggctgatag aatcaggtta cgagatccag gcagtggtaa cacagcccga
     7861 ccgtccagtt gggcggaaac gagtaattac gccgacaccg gtaaaaaaag cggctctgaa
     7921 gcacggtatc cgtgtgctgc agccagagaa aatctccggt tcgccagaaa tggaagaaat
     7981 cattgaattg gtgcctgatg tgatcgtcac tgcggcattt ggccaattct tgcctgaaaa
     8041 gttattgcaa gtaccaaaat taggggcaat caatgtccat gcttctttac ttcctaaata
     8101 tcggggaggt gcgcctgttc attatgccat catgaatggg gagaaagaaa ctggtgtaac
     8161 gatcatggaa atgatcaaaa aaatggatgc aggcggaatc tatgcgcaag aaagcatacc
     8221 aatcactaaa caagatgatg tagggacgat gtttgaaaag cttagccttt taggaagaaa
     8281 gctattgcta gaaacattgc ccaatatttt ggatggacaa aaaccagtac ctcaagacga
     8341 gtcagaagca actttttcgc caaacatcac acgggaacaa gaagcaatcg attggcataa
     8401 aactgcagaa gaaattgaca accaagtacg cggaatgcgt ccttggccaa ttgcattcac
     8461 gacctatgag ggcatacgct ggaaattatt atcggttgaa gtattggcag aaactacaga
     8521 caaagcacct ggtacgatca tccggaaaga caaaaaaaat ctatggattg cttgcggtaa
     8581 acagaccgtc ttagccatca atgaattaca gccagctggt aaaggaaaac aggccgtaca
     8641 tgatttcctt aacggaagcg gtcagcaagt tcagattggt cagcaggtga aataatgaaa
     8701 aaaatcccta aaaaaatgaa aaaatcagta cgatatatgg cattactgac attagagcga
     8761 gtgaacaagg gtggcgccta ctcgaatctt ttgttgaatg agatgatcaa acaaagcgag
     8821 ctaaatcaaa aagatgtggg tttatttaca gaattgatct atggtacgat tagccatcag
     8881 cgactattgg aattttacgt tgcaccattg atcgctaaag caaaaaaagt ggatgactgg
     8941 gtcaagatgc ttctttatct ttctgtatac caattagaat ttttagataa agtccctgca
     9001 catgctattt taaacgaggc ggtcgaaatc gcaaaagtaa aaggaaatcc tgggacaggg
     9061 aaatttgtaa atggtgtctt gaggaattat cagcgtcaag gagcaccaga tctgcaaggc
     9121 attgctgatc ctatcgaaag actttctgtc aaaatcagcc tgcctctatg gatgacgaaa
     9181 cgactggtgg atcaaatcgg atacgaagaa actgaaaagc tcggtttatc gctatatcag
     9241 cctagccatg ctagtgcccg agtagataca cgccgacttt ccagagatca ggccatcact
     9301 gttttgcaag aagaagagat cacagctgaa aaaagccaag tcagtccata cggtatagta
     9361 gcgaaaaaag ggcatttagc ttctagctca ttgttccacg atggcgtcat gacgatccaa
     9421 gatgaaagtt cgatgcttgt agcgccgtct atgcaaatcg agcctgaaca tcatgtctta
     9481 gatgcctgtg cagcacccgg aggtaagacg acgcatattg cttctttttt agatccgtca
     9541 tgcggtggag aagtggtcgc tttagatgta cacgaacaca aagtcaaact cattgaagat
     9601 aatgcagcac gtctaggtgt ctcttctgct gtctacgctc aaaagatgga tgctagagaa
     9661 gtcagtaaac agttcgaagc agagcagttt gatcggattt tagtagacgc gccttgttca
     9721 ggtcttgggc tgcttcggcg aaaaccagat attcgttata aaaaacagcc ggatgaatta
     9781 gcgaatttac caaaaatcca attggaaatt ttagaaagct gcgcaccaac tttaaagtct
     9841 tccggtattc tgacttatag cacttgtacg atcttaaaag aagaaaacca agacgttgtt
     9901 tctgctttct tggaaagaca ccctgatttt gagaggattg atgttttgac caatcctatg
     9961 ctacaatcat caatcgaaga gaaaatgttg actttgtatc cgcatcagtt ttacacagat
    10021 ggatttttca tttgttgttt gcgcaaaaag taatgaggtg aaaaaatgca gattgaatat
    10081 caatcagatg taggaagacg acgtaacaca aatcaagact atgccagtgt atttaccagc
    10141 cgttcaggtc tcaagcttgc tgtattagca gacgggatgg gtggtcatcg tgccggagac
    10201 attgccagtc agatggcggt aacgaatcta ggcgaagctt gggaaaaaca agaagctgtt
    10261 gacgatgaaa aaatcgccca atggttcatt caatcgattc aagaagaaaa tatgctgatt
    10321 tatcagcgtg gacaagaaca gccggaatat aacggtatgg gaacaacaat cgtagcggcc
    10381 gctctgtcag atgactggtt cacgattgcc aatgttgggg atagcagagc ttatttgatt
    10441 cgtgaagata aaatcatgca attgacggag gatcattctt tagtcaatga actagtcaaa
    10501 tcaggagaaa tcagcgaaga aatggcagtg aaccatccaa ggaaaaatat tttaacacgt
    10561 tctgtcggca tgcctggaac agtcgaagta gatgtttcta ctcatctttg gcaactaaaa
    10621 gatcgactgc tgttatgttc agatggcttg acgaacatgc tgtctgaaga agaaatagga
    10681 acgatcatca atcaagaagg cccattgtct cataaagtaa ctgagctgat tgatcgtgcc
    10741 aatgaagcag gcggagcgga taatatcacc gttcttctta tcgaatataa ggaggacagt
    10801 gtatgatcga gttgggcaaa aaattaaatg gccgttatca aatcactgga aatatcggta
    10861 gcggtggtat ggcaaatgta tttttggcgc acgatttaat cttggatcgt gatgtagcag
    10921 ttaaagtctt acgatttgac tttcaaaatg accagactgc gattcgacga tttcagagag
    10981 aagctttagc agcaacagaa ctcgttcatc caaacattgt tagtgtttac gatgttggtg
    11041 aagaagacaa catgcagtat ctggtgatgg aatacgtcaa aggaatggac ttgaaacgat
    11101 atatccaaac acattatcct gttccttatg aaacagcagt aaatattatg cagcagatcc
    11161 tttctgctat ttctttagct cataaccatc aaatcatcca ccgagatttg aaaccccaaa
    11221 acgtactgat cgataatgaa ggtgttgtaa aaatcacgga tttcgggatt gctatcgctt
    11281 tatctgaaac atcgatcacg cagacgaata cgatgctagg atctgttcat tatctgtccc
    11341 ctgagcaggc cagaggaagt atggcaacga agcagtcaga tatttatgct ttagggatca
    11401 tcctttatga gatgttgacg ggtagcgtac cgtttgatgg tgaatctgct gtaacaattg
    11461 ctttaaaaca ttttcaagat gatcttccat ctataaaagc acttgatcca aatgtgccac
    11521 aagcactgga aaacgtgatt ttacgggcaa cggctaaaga acctgcagac cgttataagt
    11581 cagccgagga aatgtctgat gatctttcta cagtattatc accagctcgt gcaaacgaag
    11641 aaaaatggca gcctcatgtg atggataatg aaacaaaagt tatcacaccc cttacggaag
    11701 atgcaccaat gcctgattca ttcaaatcta tgcctctgcc aaaagacaag ccagaaaacg
    11761 aaaccttacc agaggaggaa gaagttctgc ctgatcctaa aaagaaagga cagaaaaaat
    11821 ggtggctgat cctattagct attcttgctg ttttaggaat cggaacagct gtattctttg
    11881 cttctggcgg gcgaggagaa gttgttgttc ctgatgtttc tgatctttct gaagcttctg
    11941 ctagagaaac actcgcaaaa gcgaaattag aagtagcaac aaaaacagaa gaaattgcag
    12001 atgacaaaat cgaagaaggt aatgtagtga agacagaccc ggcagcggga acgacagtca
    12061 agcaaaaacg cgaagtcact ctgtatatta gtagcggcac gaaaaaaatc aaattagatg
    12121 attacagtgg taaaagttat gaagatgccg aagcaagttt gaaagaatta gggttttctg
    12181 ctgacttgat tcaaaaagaa gaggaattca gttcagaggt agaaagcgga atgatcattt
    12241 cgcaatctcc atcagaagat acagaagtcg atcctaaaac agacacgatt actttcacag
    12301 tcagcaaagg accagaaaca gtgactttga gtagtcatgt aggagaaaac tacgaaactg
    12361 ttcgtaattg gcttgttaca caaggattca atatcaataa tattagtgct acttatgact
    12421 atagcgacac tgtaccatca ggttcgatca tcagtcagga tccagcctcg ggtgaagttg
    12481 tagcagaaga aacttacatc aattttgtcg taagccaagg aacagagcct aaattgaatg
    12541 atatctccgg ctatacaaaa agcgaagcac aaagttattt agctagtgtc ggtgcagaat
    12601 atatcgggca tgagacttcc gaatattcaa acacggtgga taaagataaa gtgattcgta
    12661 cagtaccagg ggcaggaacg acaattacca aaggaatggt tgtgaacgta atttactcta
    12721 aaggaccaga tccagcgtct tctagttctg aaaaagactc ttcagacagt tctagcagct
    12781 caagtagctc cagtagcaca agcaactctt caaaagaaga atcttccagc tccacaagtt
    12841 catcagctag cacctcagaa agttcaaaat aagtgctgaa taaaatatat cagaagggaa
    12901 tgcgacattg atttgttgca ttctcttttt tgtgtttcta tctcttttga tcaattttca
    12961 agccatatac tttgagaagt ttgcatggaa taacacctac tttcctcttc gtatggtaaa
    13021 ataaacaaaa acggaggtgg cacttatcaa aggacaaatc agaaaagcaa tcagcggttt
    13081 ttactatgta tacgcaaacg gagagaccta tcaaacaaga ggaagaggaa atttccggaa
    13141 tcggaaaatc acgccgcttg tcggagatca ggtgatcttt gagagtgaaa atcaaacaga
    13201 cggctatctt ctggaaatct tgccgagaaa aaatcaatta gttcgcccac cagtcacaaa
    13261 tgttgatctt ggaatcattg tcacaagtat cgtagagcct gcattttctt ataatctact
    13321 tgaccgcttt ctagttacct tggaatacga gtcgatcgaa ccgattattt ttttgacaaa
    13381 gaccgacttg tcagacgacc cagaaaaaat cgaagagatc aagcaaacct atactaaaat
    13441 aggctatccg gtgattgtac ccaatcatac gggtgatacg acagaactga tccgttactt
    13501 cccagaacgg ctatcggtct ttatgggtca gtctggagca ggaaaatcga cgctactgaa
    13561 tcaaatatca ccggaactga atttagcaac aggagagatt tctgatgcac ttggaagagg
    13621 acggcataca acacgtcatg tggaactgat tcctatctat ggcgggttag tagcagatac
    13681 accaggattt agctcgattg actttttgac aattgaagca ggagaactgc cgagacaatt
    13741 tcctgaattt gtagaagctt ctgtccattg caaatttaga gagtgcatgc atgctaaaga
    13801 acctggctgc gaagtaaaac atagagtaga aacaggtggt atcgcacaga cacgttacga
    13861 caattatttg caattcttac aagaaatcga aaaaagaaaa ccaatgtatg caaaaaaaga
    13921 aggtaaacga aaataggagg aaacaagaat gaagatagca ccatccattt tgagtgcaga
    13981 ttttgccaat ttacaaagag acattgagtt agtggaaaaa ggaggcgcag attacatcca
    14041 cgtagatgta atggatggcc gatttgtccc aaatatcaca ttagggccaa atattgttca
    14101 ggcaatccgt cctgttacta aattaccgtt agacgtccat ctgatgatcg ttgatccaga
    14161 aaactacatt ccagcatttg caaaagctgg agcagatatc attactgtcc acgtcgaagc
    14221 aactcctcat attcatcggg cacttcaaat gacaaaaagc ttaggagtta aagctggagt
    14281 agttatcaat ccaggaacac cagtctccat gatcaaacat gttttgcctt tggctgatca
    14341 agtactagtc atgactgtca atcctgggtt cggcggacaa agctttattg aagaaacagt
    14401 agaaaaaatt acggaactcg ctgatctacg tagagagaac aattggactt atgcaatcga
    14461 agtagatggc ggaatcgttc cagaaacagc aaaaatctgt aaagatgcag gggcggatgt
    14521 cttcgttgca ggatcatata tctatggagc cgaaaatcca gtcggacaaa tcgaacgatt
    14581 aaaagaggca ttagactaaa tgaaaatttt attagctgct ggaggtaatc cgcaaaactg
    14641 gccgcagtct ctgctgtcca aatacgatgc ctatgtcggg atagaccgtg gtgcgctgta
    14701 cctgcttcaa gcaggctacc cagtagatct ggcagtgggt gattttgatt cattgtcgaa
    14761 cgaagagcag aaggaagtat ttgcaaaagc caaagagtat acccgttccc ctgcagaaaa
    14821 agacgataca gatacacagt taggtatctt aaaaacattt gaacgttacc cgcaagcgga
    14881 agtcactttg attggagcta cgggcggaag attggatcat cttttagcca atctctggtt
    14941 aggtatggaa cctcgcttca aaccctttgc ccatcagctt attatcagag attgtcaaaa
    15001 ttatttgtct tattatttgc ctggagagca tatagtaaga cagataccag atatgaagta
    15061 tttagcttat tgctgcttga ctcccgtctc gaatcttaca ttaaaaggca gcaagtatgt
    15121 attggaaaat aaagaagtag aatatccgac atcttatgca agtaacgaat ttctttcaga
    15181 taccgcagaa ttttcttttt cagatggtat gattgcagtt attcaaagca aagattgatc
    15241 cataaacaaa taaaaaaatt tgtctttcag gctaaaaatg acagaatcta ttatttgcta
    15301 aaaaaaagct taagaggagc aacttacctt ttgtttcacg gcatttagtg ataaaattga
    15361 acacaagagt ctaattaatt cgaggtgtct ttgtgaagaa gttcaatcct aataaaaata
    15421 ttattattac cttggttatt gttatcatcg tggtgacgat actaagtgtg accgctgcgc
    15481 agcgagcgaa tgaaggaaag cccaatttct tccaatccat cgttaatgac agtgttgcaa
    15541 tggttgataa aacaatctct gccccagtac ggtgggtacg aaatggagta gattctgtcc
    15601 ataatctttt ttcaacttat gccgaaaatg agcggctaaa agaaaaaatc gatagctatg
    15661 atgacgtttc acagcaaaac aagaattacg agagagaaat cgaagcgttg aaaaaagaac
    15721 ttgagttgaa tcagacgctg actagttacg aaaaagtaac ggcaaatgtc attactcgtt
    15781 cgcctgacag ttggcaggac atgctgatcg tagacaaggg ttctaaagac ggtatcgaag
    15841 caaatatggc agtcatgtcg caaaaaggat tgattggccg agtgatcgag gtcaatacgg
    15901 cttcgtctaa aatcgaatta ctgtcatcct ctaatgaaag ctccaatcat tttccagtac
    15961 gggtatcttc ggctaatggc gaagcgtttg gtttgcttaa aaactatgat gaaaagctcc
    16021 atgccttagt ggtgacccaa ttaactggtg atacggatat caaagaaggg gatgttgtcc
    16081 agacatccgg tcttggaggg aattctccag ctaacttgcc gatcggtacg gttattaaaa
    16141 cgaaaccaga tagttatggg ctggatcggg aagtttatgt gaaaccttat gcagaaatgt
    16201 atgacgtgtc agttgtgacg attgtccaac ggctagcgga ggattagtcc atgttgaaga
    16261 aagaaacaat gaagtactat ctgccaatcg ttttgttctt tttgatgttg atagatggtc
    16321 atttaacaag aatgctaggg gagtggtcga aaggcaccta tatgtcaaat gcccactttc
    16381 tgatattggc attattatgt tgcagtatgg cgtttgaaaa acgttattta ctgattacca
    16441 cgattgttct cggggctatc tatgacgctt actatattgg cgttatcggt atctatgcag
    16501 tagctctccc tttaattgta tggttgatgt atgtaatgaa agacgttatc catgtcaaca
    16561 tctttactga atttttcagt atgatcatct ttgtcacggg ttatgaattg tttacgatgg
    16621 tggtccagtt gatttttaaa ttagcagtag taaataacac gtattttatt acaaggtttt
    16681 taggacctac actgctg
//
LOCUS       DAJSKS010000055        16529 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJSKS010000055 DAJSKS010000000
VERSION     DAJSKS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.056"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(24..722)
                     /locus_tag="ON396_001496"
     CDS             complement(24..722)
                     /locus_tag="ON396_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCR3113587.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="ON396_001497"
     CDS             complement(715..2067)
                     /locus_tag="ON396_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HCR3113588.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="ON396_001498"
     CDS             complement(2290..3690)
                     /locus_tag="ON396_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCR3113589.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="ON396_001499"
     CDS             3851..4588
                     /locus_tag="ON396_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR3113590.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="ON396_001500"
     CDS             4713..5192
                     /locus_tag="ON396_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HCR3113591.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="ON396_001501"
     CDS             5189..5956
                     /locus_tag="ON396_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113592.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="ON396_001502"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="ON396_001502"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR3113593.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="ON396_001503"
     CDS             complement(6859..7461)
                     /locus_tag="ON396_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR3113594.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="ON396_001504"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="ON396_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="ON396_001505"
     CDS             8142..8726
                     /locus_tag="ON396_001505"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR3113595.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="ON396_001506"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="ON396_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR3113596.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="ON396_001507"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="ON396_001507"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR3113597.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="ON396_001508"
     CDS             10627..10806
                     /locus_tag="ON396_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113598.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="ON396_001509"
     CDS             10828..11853
                     /locus_tag="ON396_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR3113599.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="ON396_001510"
     CDS             12028..13272
                     /locus_tag="ON396_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR3113600.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="ON396_001511"
     CDS             13425..14372
                     /locus_tag="ON396_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3113601.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="ON396_001512"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="ON396_001512"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR3113602.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="ON396_001513"
     CDS             complement(15274..15516)
                     /locus_tag="ON396_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113603.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="ON396_001514"
     tRNA            15637..15709
                     /locus_tag="ON396_001514"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
     gene            complement(15828..16466)
                     /locus_tag="ON396_001515"
     CDS             complement(15828..16466)
                     /locus_tag="ON396_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR3113604.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5639 a         2949 c         3324 g         4617 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat
    15781 aaagtatgct ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa
    15841 acgactagca atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc
    15901 gagttgcttt taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg
    15961 atgtgtgcta cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca
    16021 aagatgattg ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg
    16081 agaaaataca ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat
    16141 aacgggctga aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc
    16201 aaaaataccg agactgcggc actgattact acattgataa aaaaatattt ggctatattt
    16261 ttgacaggag cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt
    16321 gtaaatgcta taaatccagt gatagttaaa gaaaaaatgc taataatgga tggcgcaaat
    16381 agcaacccta acaaaccgag tacaacgaca atagtgccca atatcccttt tttaagttct
    16441 attttttttc ctaaaatgtt attcatgata ataactccta ctattcagtt tttgaagata
    16501 gagtcagtat acatgaagag ttaaaatag
//
LOCUS       DAJSKS010000056        16212 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJSKS010000056 DAJSKS010000000
VERSION     DAJSKS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.057"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..469
                     /gene="scm"
                     /locus_tag="ON396_001516"
     CDS             <1..469
                     /gene="scm"
                     /locus_tag="ON396_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCR3113605.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTS
                     DSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNF
                     NLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(624..1610)
                     /locus_tag="ON396_001517"
     CDS             complement(624..1610)
                     /locus_tag="ON396_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCR3113606.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1633..2199)
                     /locus_tag="ON396_001518"
     CDS             complement(1633..2199)
                     /locus_tag="ON396_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HCR3113607.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2213..2629)
                     /locus_tag="ON396_001519"
     CDS             complement(2213..2629)
                     /locus_tag="ON396_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCR3113608.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3086..5020)
                     /locus_tag="ON396_001520"
     CDS             complement(3086..5020)
                     /locus_tag="ON396_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3113609.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGKSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5301..6257
                     /locus_tag="ON396_001521"
     CDS             5301..6257
                     /locus_tag="ON396_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HCR3113610.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6347..7450
                     /locus_tag="ON396_001522"
     CDS             6347..7450
                     /locus_tag="ON396_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCR3113611.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7463..8416
                     /gene="menA"
                     /locus_tag="ON396_001523"
     CDS             7463..8416
                     /gene="menA"
                     /locus_tag="ON396_001523"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR3113612.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9230..10576)
                     /gene="gor"
                     /locus_tag="ON396_001524"
     CDS             complement(9230..10576)
                     /gene="gor"
                     /locus_tag="ON396_001524"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR3113613.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10710..11963)
                     /locus_tag="ON396_001525"
     CDS             complement(10710..11963)
                     /locus_tag="ON396_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR3113614.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11969..12892)
                     /locus_tag="ON396_001526"
     CDS             complement(11969..12892)
                     /locus_tag="ON396_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR3113615.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13113..15605)
                     /locus_tag="ON396_001527"
     CDS             complement(13113..15605)
                     /locus_tag="ON396_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCR3113616.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15612..16079)
                     /locus_tag="ON396_001528"
     CDS             complement(15612..16079)
                     /locus_tag="ON396_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCR3113617.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4763 a         3391 c         2880 g         5178 t
ORIGIN      
        1 cacatcagct tcaagtgaca gtacagatac tacctctacc acaagtgata gcacggacac
       61 gtcagcttca agtgacagta cagatactac ctctaccaca agtgatagct cagattcatc
      121 taccactaca agtgatagca cggacacgtc agcttcaagt gacagtacag atacatcctc
      181 taccacaagt gatagctcag attcatcaac ttcaagcgat agtacagaca cgtcgtctac
      241 cacaagcgat acacgtcatg caagtgtctc cttctcaaaa acaagttcgg ataacaggac
      301 gaactttaac ttaactaata aaacaagcaa tgacaatgga aacaaatcaa aaagagcact
      361 tcctaaaact ggaagtcaat cgaataattg gataacactc gcaggcgtta tattacttgt
      421 aatcggttta agaatcactt ttatgagtta taagaaatcg aaaaggtaaa aaaatattca
      481 agataaaaaa gagcaacgtc gctaacggac gttgctcttt atttttgctt tttatcataa
      541 atacaagaac tacaattcaa tttgtcaaaa aacacgtaga tttccttctg agaaatttat
      601 ttacgtatcg aacctttccc tattcagtct ttgcgatcca aaatcagttg ggtaagggaa
      661 aagagattct cttttgttcg aagagaggtg gcaggtaatt tctttatttc cttcaaagcc
      721 ttttctgtgt aagaggccgc taacttccta gcttcttcca cgcctcctgt atgttcaacg
      781 attttgcgta cggcgtcgac atcttcttct gtcattgccg ctttcttctc taaataaggt
      841 agtagctttg gtttgttttt ttgcaaagag tagatcaacg gtaaagaata gaccccttga
      901 cgcatgtctt ctagtacagg tttgccgatt tcctggctct cttttgtata atctaaaata
      961 tcatcgataa tctgaaaggc catcccgata gaaagtccga tatctcctgc tttttttgcg
     1021 aagcgctcgc ttgttccact ttcaaaagcg ccaacagaac aacttaattg gaacagttca
     1081 gctgtcttac ctgaaatatt ttccaaatat tcttttacag aaagttcata attatagcga
     1141 tcatccatct gccctaattc gccattcaat actttttcca tactgcgaga attcaattgg
     1201 agacttttca aggaagtgga gtaatcagac aatagtttaa aacagcagac aaataaatag
     1261 tctcctgcat agacagaaac gacattacca aactgactac ggatattagg ctgaccacgt
     1321 cgcagatccg cttcatcaac gatatcatca tggatcagtg tggccgtatg gagcatttcg
     1381 atagaagctg ccaaagcgat ggctttgtct cgatcttgct ctggtccaaa ttgtgagaac
     1441 aatagctgat aggcaggacg caacagcttt ccgccggcat ggatcgtttc tttgattgct
     1501 tgttcgacag gtttgttttt caggcggaca gtttcttcca tccgttttag tgtttctgtc
     1561 aattctttac ttaaagcagg atagttttcc cacattggat gtagcttcat aaaaaaactc
     1621 cttgtgtgac gattattttt tggtaaacat tgggctccat ttttgtttgg ttccctgctc
     1681 ataatctttt tggaatcgtt gaagtgtctg cacatgtcgt aaaagataat tcgctgcgat
     1741 cccgattgca atccctgata aaatccctaa aactgaaagg actggcaagt acaacatgac
     1801 tgtccatgat tgtgcaatga aagaagccat caaaagctgt ccgacattat gcatgaaacc
     1861 gccggccgca ctaattccga tgatactgac acgttttggt cctaattgct tgaccaataa
     1921 cattccaaaa taactaagca aagcaccact catactataa aggaatgtgg aaatcgttcc
     1981 gcctagcaaa gtagtcagga atagtcgtag ccaaagtagt aagaagctgt cttgtttttt
     2041 catggtaaaa atggcaacga tcgtgattag attggctaag cctagttttg caccaggcgc
     2101 aaaagaaaaa ggataaggga tcatattttc caataaacca atgatcactc cttgtgcaac
     2161 aagtagcgaa atataaatca ttttttgtaa tttactcatg gtgtgtaaag gtctaataaa
     2221 tcagactgtc ttcgtcacct ccatctgttg aacggacttc gatcactaat ttatgtggca
     2281 aacagacgat ggtttcgcca tttttcgttg cccagcctcg acgtacgcag atctggtcat
     2341 cgcaatctgc ttcttttatc cgtactcgat cgccatctat ctcgatcaag ttataatctc
     2401 cgtgttcatc tttgtaagta tatgtgttgc tctcttctcc agccatcaaa gggaatgttt
     2461 tgatttctgt gccatccaca cgcagtaccg cttctttttc tgctgttgcg gtgttttgct
     2521 gaagaccaaa aatcatgact ggtaaaaaag aaagaacagt caaaagcaaa ataataaaaa
     2581 catcccaagg tttgagcaga cttttttcag caaattcttt aaggctcatt tttgttctcc
     2641 tttttccaga ttaactcacc caattttagc ataaaaacaa gaggttgtga cacctgtgtc
     2701 ttgctttgag taataagaac gaagaataga aaacagcttt ccatgttttc taattctttg
     2761 ggcttattac cgagaagcag caggtggaac accgtttata acaagaggtt gtgacagaag
     2821 tgggcaactt caagaaataa gaaggaattt acgaaaatta cttttcaagt ttttgtgaaa
     2881 tttcggctta tttttcgaag aagctacttc tggaacaccg tttataacaa gaggttgtaa
     2941 cagaggttgt gacacctgtg tcttgctcat ggaggcttat ctcgggcatc ttattccttt
     3001 aattttacat aaaaaggagg ctgtgacggt ttgtctcagc ccctttctat tgaaatcttg
     3061 cattcaaata acgttcttta tctatttatt tcttgccgta ctgggaactt ggtctaccgt
     3121 accaccaatt acctagtgta cgttgtagcc atggaattac ccagcgatcc aacccaatcg
     3181 cacgtccgga accgttcatc aatgcgaaag caacaaaaat gaaccaaatg tttacccagt
     3241 aaaacatgcc tgataagcag aacgtgatca ccaatacaat cgttgtcgca ctgcatagcc
     3301 atgtgaataa tccagcaatc aatgctaatg ccaaggcaat ttcaaagaat accatgaatt
     3361 tttggaagaa cattgctaca tcattgtttg gcatgatcac ttgcatgatc tttgtgaacc
     3421 agtctggtga cttctcaatc acagccattg gttcttcacc gtatgcataa ctcaaaccaa
     3481 aatgtgcagc ttgagtcgct gcttctccgg cacttgcccc tgctccgctg gcagctgtgg
     3541 ttgcatcacc agcagcagaa gcaccactgg caacatctgt tacattgtat tcccatgtcc
     3601 aagggaacac actttttcca gtgaaccatg atccttcacc aaaccaagta cttggtttga
     3661 acacttcacc attcccaatg atttttttgg aagcttcaac gaaccaaact aaaccataga
     3721 aaatacgaag tggcacactc cataagacat tgccgtaacg agaagaatgt cctcgagtca
     3781 cgttgcgatc atctttgata tggaaaaact catgcattaa atattggaac atataataac
     3841 cagaacgaat atcaaagaag tatttcaaat tcacgatatg tttcattacg atagctaaaa
     3901 atccgcttaa atggatctta tcaaataggt tagctacccc ccatttggca ccaatagaaa
     3961 ccataaatcc ttgatagttt cctttaaatt tatgcttttc tccgcctttg atcgaagcca
     4021 cgatattcgc tgcggctgtg tgtcctgttt gttcagcagc ttgaacgatc tgcggtgtcg
     4081 gtgttcctgg gaattcttca taaaagacta aatcaccaat gatatagatg tttttatctt
     4141 cgtagccttt tgcctgcata tattcattgg cgatcaaccg ctgtccgcgt gctgattcta
     4201 atccaaagtc agcagcgtca gaagttcctt tgacaccagc tgtccaaatc agtgtgtgag
     4261 tcggaatgtt gcgtccgtct ttcaatttga tatgatcagc agccacttct acgattggtg
     4321 cattcaataa taattccacg ttgtgcttac gcaaatagcg ttcagctttt gcagcatcat
     4381 ttcgatccaa catattcaag atcgtcggca ttgcctctac gaccatcagt gtgatttcgt
     4441 ttggatctaa tttataatct ttggctaggc gatttttcca gtcgatcagt tccccaatca
     4501 tttcaatacc ggtaaatcca gaaccacaga caacgaaagt cagcatggct ttacgcagtt
     4561 catcatctgg ttccaatgca gcttttgcta ctgtcttctc aatatgttcc cgaatcctta
     4621 cagaatcttc aaatgaccaa agggtgaatc cgtgtgcttt gacaccaggt gtaccaaagt
     4681 cattcggctc tccgcccatt ccaaggatca gctgatcaaa aggataggaa cctaatttgg
     4741 tcttgacgat ttttcgttct ttatcgatac cgattacagt gtctgtgaca agttgtacat
     4801 tcttttgacg agcaaacaaa tgctgcagat cgtattggat tgcttctggt tctacacgtc
     4861 cgccagctac ttcatgcaac tcggtcatca tagtatggta cgaatgacga tcgatcagag
     4921 taattgctac atcttctttt ttcagtttct ttgccaagta ttttgtagct gaaacaccgg
     4981 catatcctgc tcctacaacg acaatatttt gctttgccat tgatcatccg ctccttatag
     5041 actatctata taatgaatat atgataattc ttttagtttg atacttatta cacaatctcc
     5101 atatattata tagatgttct gttcttttgt ctaactcctt ttggattttt ttcaaatttt
     5161 aaaataaagc gttttttcac gaaatttgct gaaagaaaac gtttgaaata ttacatgggt
     5221 tatttatcaa aaatagttga cttataggct aaaaagatta gaatgtataa tgaatcacaa
     5281 agaaaaattg tggactagaa atggaatggt ggggtattaa aatgaagaaa aaacgttttg
     5341 taactggatt tgccgtatta gcattttcag ctctagtgtt aggcgcttgc ggcgcagatg
     5401 acaacggaag ctcaaactct tcttctgaat catctacagc acaatcttcg actgcaaaaa
     5461 gcacaacaga atcatctgca aaagttgtag ctggcggaga tttgcaagac ggcacatata
     5521 aattagaaga aaaaaattat tcaaatggtt accgtgcagt ctttgaaatg gttgtaaaag
     5581 acggcaaaat cactgaatca aaatatgata acgtcaatga aaacggcgaa tcaaaaacaa
     5641 aagatgcaga atacaacaaa aacatggaag caaaatctgg cacaaaccca gaaaaataca
     5701 ttcctgagtt gaatgaccaa ttcttaaaag cacaaagcgc aagcggtgtg gaagtcgtaa
     5761 ctggagctac tcactcatct gaaagcttcc aaaactacgc acaacaattg atccaagctg
     5821 ctcaagctgg taacacagat acaatcgaaa tcgacaatgg tgctgatttg aaagacggca
     5881 cttacaagct tgaagaaaag aactactcta acggctaccg tgtgcaattc gaaatgacag
     5941 ttgctggcgg caaagtaact gaatcaaact ttgattatat cgacaaagac ggtaaatcaa
     6001 aacaagatga tacagaatat aatgaaaata tgaaagcaaa aagcggtaca gaaccaaaaa
     6061 cttacatccc tactctgaac gacgaattcg tcaaagcaat gggcgaagaa gatggctcac
     6121 ctgctaatgt agaagttgtt acaggtgcta cacacagctc acattcattc atcatgtacg
     6181 cacaacaatt agtgaatgca gctgaaaaag gcgacactca aacaatcgaa gtagataata
     6241 tcgtaacaga aaaataatca cataatcata ctaataaaag ttggagggtg ggacgatagc
     6301 cagattaggc agtcgtctca cctttcaaaa tgaagagagg aaaaagatgc ggaaaaaact
     6361 actattttcc ttaatcacac tgggcatctc ttttatggtg ctaactggct gcagcaactc
     6421 agcaaataaa acaaccactg aaagttcagc agctaccaag atattgaaag atccatattc
     6481 tgatgaacag ttcttgttag ggacttacgt tcgtatccgt gtgtatgatg aaggcaaaga
     6541 atcagcattg aaacctgcct tcgatcgagt aaaagaactt ggtgataaaa tcacgatcaa
     6601 tcaaaaaggt tctgagatcg atgaagtgaa tgagcaagct gggatcaaac cagtaaaagt
     6661 ttctgatgac gtctacacgt tagtcaagcg ggcctacgaa tatagccaag attctcaagg
     6721 cggattcgat atggcgattg gtgcaatcac acaattatgg cgaatcggtt ttgatgacgc
     6781 cagaaaacct tcccaagaag aaatcgatca ggcattgaaa ttagtggatt atcataagat
     6841 cgaactaaat gacaaagaaa aaactgttta tctaaaagaa aaaggaatga tcatcgattt
     6901 aggtgcaatc gccaaaggtt acattacgga cgaagtagtg aaagtcttga aaaaacaagg
     6961 agtgaccact gccatcgttg acttgggcgg taatgtctat gtcttaggcc acagcccaag
     7021 aggcgaaaac caagactgga cagtcggcat ccaagatccg aacatggctc gcggatctgt
     7081 tttaggttct attaaagaac ggaacaaaac attagttacc tctggaattt acgagcgtta
     7141 tttagaagta gatggcaaaa aataccatca tttgtttgat ccaaaaacgg gttatccgtt
     7201 cgacaatgat atcgcaagtg tcacgatcat aacagataaa tcaattgatg gtgacgggtt
     7261 gtctacagca gtcttttcaa tgggtgtgaa aaaaggactg gaatacgtcg aaagcgaatt
     7321 gaataacggg acggaagcga tcttcgtgac aaaagacgat aaagtatatg tgaccgatcc
     7381 aatcaaagac accttcaaat tatcggagga ctcccattac acgatgggtg accgttctga
     7441 actaaaataa ggagtacgaa ccatgacact aaaaatcttt ttagaagtag ttgaaatacg
     7501 aacaaaggtg gctagtgtat ttccttttgc ggtaggggta ttattctcta tcgctttttt
     7561 tcatgagata cattggctaa acactttttt attttttatt ggaatgctag tttttgattt
     7621 agcgactacc gctatcaata attacatgga ttatgaaaag gctcattctg aagtatataa
     7681 gtatgaagaa aatgtcattg gccgcagaaa tctatctcct gtattgattc gaaacatgat
     7741 tttagctatg attgcatttg tttttgttat cggcgttatt ctaacgatca atacgggttg
     7801 gcttttactt ttgatgggat ttatctgttg ttttattgga gtattctata cattcggacc
     7861 gattccttta tctcgaatgc ctttagggga aattttcagt ggggtgacta tgggccttgg
     7921 aatttttgcc atggtcgttt atgtcaatac attcggttca gatgtctatg atctgacagt
     7981 caacttttca tctggacaat ttgctttggt aggaaatctt tgggctatag cagcaatcgt
     8041 attagcttcc ttacccttgg tgtttacgat tgccaatatc atgttggcta ataatttgcg
     8101 ggatcttgac cgtgatattg aaaatcatcg ctatacacta gttttttata tcggacgtcc
     8161 tgcgggaaca gtcttattcc agctattgat gtatgcttgc tatttcgtga ttttaatggg
     8221 gttgttgagc ggtatctatg aatggccgat cttggtcacc ttcttgacct tgccaattgt
     8281 gaagaagcat ttggatcaat tcaagcaaac gttaccccag cctatcagtt tcagctattc
     8341 catcaaaaat atggtcttat tcaatgccag ttatgtattg gggctattac taacaatcat
     8401 tatgcaagcc atttaacagc atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata
     8461 agaacagcca aataaaaata gctccttata tttttcgttt ggttgggctt attaccgcag
     8521 atgcaagctt ttgaacaccg tttatcagga ggttgtgaaa aagtcgctta gtttcgagta
     8581 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttgg cttaataccg
     8641 agaaactggc ttttgaacac cgtttatcag aggttgtaaa atcagtgttt tgtcctgagt
     8701 attagagaaa caaatggaaa aatagttcct catattttgc cgttttttga gctaataccg
     8761 aaggacagct gattgaacac tatttgttcg gaaaaagtga ctaactatga cacaattttt
     8821 ttcacagtcc cttttttcac ttgatttgat tcgtaccagc gaacttgacg agaactttct
     8881 tggtttggta actatatggt attcttttaa catcctcgtt tgcttcttct aatttattta
     8941 tacaaagtca cactttttta aacatctgtt cttcgataca tttctatttc ttttaaacat
     9001 cgataccaat attttgaact taagagtgca taccaaatgg attcatttac tcttcgtcag
     9061 ttacgaagat aatcaaaatc tccaacttcc ctacttattt cgttttgaga attatcgaag
     9121 caatcttaca taaataacta aaaaacaaaa aaccagaaaa tcagtatact gattttctgg
     9181 ttttacatgg tttgttcaat aaaaagctgt tggaactgtt aatattaaat tatcgcatcg
     9241 ttacgaattc ttccgctcct gttggatgga tggctactgt attgtcaaag tcttctttag
     9301 ttgcacccat cttgatagcc acggcaaaac cttgcagcat ctcatcgaca ccaacaccaa
     9361 tagcatgtaa gccgacgatt ctttcttctt ttccgacaca gatcaatttc atttcgcatt
     9421 tttggcgata atcatttaat gcaaagtaca ttggggtaaa gcgtgaacga taaactttca
     9481 attcattttc gccatatttt tccaatgctt cttcttctgt caaaccaatt gttgcaattg
     9541 gtggatgcgt aaagacgact gtcggaacaa gtttatagtc aagataagaa tctttctttc
     9601 cattaaataa acgttctgat aaacgtctac cagctgcgat tgcaacagga gtcagatcta
     9661 atttaccgat cacatcacca acagcaaaaa tatgttcatc cgttgtattt tggtatttat
     9721 ctactttgat aaaccctttt ttgtccaatt ccacatttgt attttccaga cctaattttt
     9781 cggtattcgg tacacgtcca ccggcaaaaa gtacacaatc accagttaag gtttcaccat
     9841 tttcaaatag caccgtatat tcgtcattgt ttttagtaat ttctttagca atatgatgtg
     9901 cgtaagtttt tattccagct tcttggtaca tttggatcaa attgtccgaa agcattttat
     9961 caaacgttct aagtggtcgt tcttttcgat atgcccacat gacttcacta cctaaaccac
    10021 gaaatacacc tgaaagttca gccgcaatgt atccagcacc taaaacgatt gctctcttag
    10081 gtaactctgt taattcaaaa aatccatttg aatcaattgc gtattctcca ccaggaatgg
    10141 tcattttact tgcacggcct ccagtagcga tcaagatttt aggagcacga taggtttcac
    10201 cgtttacttc gatcgtttga ctatcgataa aagtagcgta accttttata gcctcgacat
    10261 gattgctatc taatcctctt tgataagcac cgtgtagaaa atcaatatat ttttctcgtc
    10321 tttctactag accggcgaaa tcaacatttt ttaaatctgc ttggataccg taacttgagg
    10381 catctcgttc aattgtttcc aaaatcgtac ttgcttgcca cataactttt ttaggtacac
    10441 aaccgacgtt gacacatgtt ccgcccaatt cattcgcttc aatcagcaaa acttttgcgc
    10501 catgcatccc tgctctgttt gctgaagcaa taccgccaga gccgccacca attacgatgt
    10561 aatcatatat ttgcattttg ttcctcctta ttgaagagat caatcaaaac ctttatttaa
    10621 cgatcctttt tctacagaaa acaagtgtaa cacttttctt gaattttgta tgcgaagatg
    10681 cttaaaacag ggatatatca gctttttcct tatttacgtt ttcggatcat atctccaggt
    10741 ttcaccggct gtgcgactgg catagttaaa atcatcattg gatttggtgc tcggtcaatg
    10801 gattcgcctt cttcattatg cataacttca acggtttgac tgaaatgact gaatcctgga
    10861 ccataaaatt cgatttcatc tcctacagaa aacaagtttc tctgacgtat cgtagcaatc
    10921 tgtgttgcct catcataaga taatacttca ccgacaaatt tatatactgg gattttacga
    10981 cgttcaccga ataattgttc gttttcagtt ggtgtattgt agtaaaaacc tgtagctaat
    11041 tctcgttgtg ctactttcca taactcgtcg atccattctt gcttgcagac atagttttca
    11101 ggatcttcca tataactgtc tacagccgct ttatagacat ttgacaccgt agatacataa
    11161 tggatcgatt tcattcttcc ttcgatcttg aagctgtcta cgccgttttc aatcaattct
    11221 ggaatatgtt cgatcattga catatccact gcactcatcg agaattcttc ttccacttcg
    11281 cctgtatcag ttaaactgtt tcgttcttga ccaaaaggca tatcgaacaa tccatacttc
    11341 cagcgacaag attgagaaca tccgccacgg tttgcatccc gcatcgacat atggttggac
    11401 aatgtgcatc gaccagaata agagatacac atggcaccat ggataaatgc ttcgatttcg
    11461 gtatccgtat ttttacgaat ctctgccact tcctccattg aaacttcacg agctaaaacg
    11521 acacgttcca acccttcttc tttccagaat tccaatgtct caaaattagt tgctgaagct
    11581 tgagtggata aatggatcgg cagacctggg gcttctgtcg cacaaatttc gatcaaagct
    11641 ggatcagaaa caatcactgc agaaataccg acatcacgca aactgcggaa aaattcccct
    11701 gctccatctt catttccttc atgggtgacc atattagcag caacgtatac cttcgcatca
    11761 tgttcttttg caaaagcaac tccttcagcc atttcttcat aagtaaaatt acctgcacgg
    11821 cttcttaaac catatgcatt cccgccaata tatactgcat ctgctccata atggatcgcg
    11881 gtttttaact tttccagtgt tcctgctggc gctaggattt ctggtcgttt caattttctt
    11941 tttactgtca tttctttcat catactcctt atttgatagc agaaggatca atataaaaga
    12001 atcctgtatc taattcacgg ttttcaggat gcaatttact gatttcgcta gtcatttttt
    12061 ccgctttttc ttgatcccag ctaccttcta aaatttgttt ttttgcttcg gcgaatagtc
    12121 gagtgatgtt tacgaatttt tcgcctggag taaataatcc gtccaatttc catgtacagt
    12181 aatgatgctg ataaagttca ttcaattcat tcgagagatt cagatcattg ctggcaaaaa
    12241 tatgtgtgcc atgactatct tcataaatag aataatgtgt ctcttctttt ttaggttccg
    12301 agataaaaag acctctttca cgatcttttt gttcgtcttg tttcgtatag ttatagtaat
    12361 tctgtagcag tggacgtttg gattgatgga tgcatgtagc accatacaca agggtttcta
    12421 caggaatatc gagtttttct tccatcgcct tcatctcttc aaaaggaact tcacgagcaa
    12481 gtactgctcc aactgcgcct tttttcgccc agaaattgat ttgacgtgcg ctcgttacta
    12541 atgtttcagc atcataaata tacggtatac gcaattcagg attttttttc atcgtataaa
    12601 taatacctgt atcaccaact gtgatcacat ctacgtttat ttcttttaaa aaagccaaat
    12661 attcaggtac atttttcatt ttttcaggat gaatgatccc atttacagca atattcgctt
    12721 ttttaccata cttgtgtgct aattctacca attgtcgctg ttcttctcgt gtaaatgaag
    12781 caggcagacg caatccaaat tgctcttcac cgaaatatag tgtatctgca cctgcagcaa
    12841 gcaactgttc tgcttgttca atagtttcta ctgtggttat gagttcgatc ataattttct
    12901 cctcctacaa cttcataata gtagcatagc tctaaaaact ttccaatact tcttctattt
    12961 aaagtcggat ttttgaaaat tatttcacaa aaaatcaaaa taaagttcga tatattctgt
    13021 ccaaatataa aaagagattg tgacaaaaat cttgtcacaa cctcttaatc aagtcttact
    13081 attataaaaa ttcagctaca caggccaagt aactataggg aacctactgt ttccttttta
    13141 ggtccgcgaa cattcaatgt gatcttacct tttgaagcac ctactgttac gtggtcacct
    13201 aaacgaattt ctccgccaag caaagcctcg ctcaatcgat cttcaatttc tttttgaagt
    13261 gcgcgacgga ttggacgtgc accatattca ggatcaaatc cagctttccc gatcacatcg
    13321 attgcagatg gtgtgatttt cacttgaata tcttgttctt tcaatcgttt gataatcgct
    13381 ttgctcatga tttggacgat tgtatggatt tcatctttac tcaatgaatg gaagacgaca
    13441 gtctcatcga ttcgattcaa gaattcaggt cggaaagctt tctttaactc ttctaagata
    13501 cgtttttgca tggctttgtg atctttagta acatcttgga cattgaatcc gacatttttt
    13561 tcttctcgaa tttgtgtcgc tccgatattt gaagtcataa tcatgattgt atttctaaag
    13621 tccacttttc tacctttaga atctgttaaa tgaccatcat ccaatacttg cagaagtaaa
    13681 ttgaaaacgt ctggatgtgc tttctccact tcatctaaga gaatcactga atatggtttt
    13741 tgacgaattt tttctgtcaa ctgtccacct tcatcatacc ctacgtatcc tggaggagac
    13801 ccgattaatc ggcttgtact gtatttttcc ataaattctg acatatcaac acggatcagg
    13861 gcatcttcac ttccaaacat gacttctgaa agtgctttgg ccaattctgt ttttccgaca
    13921 ccagttggtc caaggaacat gaatgaacca attggacgat caggatcttt caatccgctt
    13981 cgtgcacggc ggatcgcacg tgaaactgct ttgacagcct catcttgacc aacgacacgt
    14041 tcatgcagaa gtccttccag ttctaacaag cgttcgcttt cttttttctc caactgctgt
    14101 agagggacac cagtccactc cgatacaact gtagcaacat cttcttctgt tacacgatca
    14161 gaataacctt gctcctgttt tgcttcaatt gcagaaactt tttgcagctt tttacgtagt
    14221 gctttttctt gtctgcgtaa ttgagcagca ttttcaaagt cttgtttttg gatcgctgct
    14281 tccttttctt gtactagttc atcgatttct agtttgatca cagttgattt tgttaagtga
    14341 tcggtttgat ccaaacggac tttcgctgct gattcatcga tcaaatcaat tgctttatcg
    14401 ggtagttgac gagaagtgat ataacgaaca gaaagattga ctgctgctcg tacagcttca
    14461 tctgtgatct ccactccatg gtgctcttca tatcttgtac gtaatccttg aagaatcact
    14521 tctgcttctt caggagttgg ctcatctact tgaatgcgag caaaacgtcg ctctaatgca
    14581 gaatcttttt cgatatattt ttgatattca tctagggtcg ttgctccgat cgtttgtaat
    14641 tctcctcgag ctaacgccgg tttcaagata ttcgaagcat caattgctcc ttctgctcca
    14701 cctgcaccaa ttaaggtatg caattcatca atgaataaga ttacttgacc atcattatag
    14761 atttcgtcga tgaccttttt cattcgatct tcaaattcac cgcgatattt cgtaccagca
    14821 accaaagatc ccatatcaag catcatcagg cgcttttctt gcatgtcttc tggcacttca
    14881 cctaaaatga ttttttgagc taacccttca gcaatggctg ttttgcctac accaggttca
    14941 cccaccagta cagggttatt ctttgtccgt cggctaagaa tctggataag ccgtttcact
    15001 tctctcgaac gaccaacaac tgggtccagc ttgttttctt ttgctaattt cgtcaaatca
    15061 cgtgctaatg aatctagcgt aggtgttcct tgcggtgttt gccgtttatc ttgtacattt
    15121 acatttctac gacgattcat tccttttcca gcagaatcac cagctagccc cattttttgt
    15181 ttcagaactt gacgggcttt tgaaaggctg atacctaagt tgatcatgat tcgagtagcc
    15241 atgatacttt catcttttaa taagctcatc aaaatatgtt ctgttccaac tttttgagaa
    15301 ccagttttct tcacttcgct gctagctaaa gcaaaaactt gtttcattct tggtgaatat
    15361 ggaagaaaga ctcctttagg gtaagaacgg ataccaccat aaccaatcag atgttcaatt
    15421 tcttcataaa tttccgtttc attcaaatcc atttcacgta atacttttcc agcaattcca
    15481 ttttgctcga ccactaaggc tagtaaaaca tgttctgaac taactgattg atgtttcaac
    15541 cgttttgctt cttcttgtgc aatttctaat actgcttggg ctctttcagt aaaaagttct
    15601 tccatctaat ctcacttccc atcttcataa cttaaacgtt ccagaaatgc tcggagaatc
    15661 cgagcacgtg tatgatcttc atattcattg aaattcaatg tgttttttgc aattgcactc
    15721 aacatcaagt ttccttcttt ttttgtaatt atttggtctt cataaagttt ttgaataata
    15781 gcaaaagcat ttttctcact gattgtatca tcaaataact gatttatctg ctcaagtaat
    15841 tgttttttat ctgaaatcct tacttttgca attcgtatat aaccaccacc accacgtttg
    15901 ctttctacta gataaccacg ttggatggta aaacgggtat taatcacata gttgatttgt
    15961 gaaggaacac agttaaagag gtctgccatt tcaatccttc gaatctcgat catttcacta
    16021 ttttccagaa ttttctttat atatgattca attaaatccg aggtattctg atgtgccatc
    16081 ttattcattc cctttgacta atattgacct taattataac gaatttgata atagaaagaa
    16141 agaagaacgc tttttgttaa ataaatcctt tagaatcaac aaaaaaaaat acggaatata
    16201 ctattccgta tc
//
LOCUS       DAJSKS010000057        15939 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJSKS010000057 DAJSKS010000000
VERSION     DAJSKS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.058"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            142..1167
                     /locus_tag="ON396_001529"
     CDS             142..1167
                     /locus_tag="ON396_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3113618.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(1762..5436)
                     /gene="rpoC"
                     /locus_tag="ON396_001530"
     CDS             complement(1762..5436)
                     /gene="rpoC"
                     /locus_tag="ON396_001530"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR3113619.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(5494..9111)
                     /gene="rpoB"
                     /locus_tag="ON396_001531"
     CDS             complement(5494..9111)
                     /gene="rpoB"
                     /locus_tag="ON396_001531"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR3113620.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            9839..10807
                     /locus_tag="ON396_001532"
     CDS             9839..10807
                     /locus_tag="ON396_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3113621.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            10825..11730
                     /locus_tag="ON396_001533"
     CDS             10825..11730
                     /locus_tag="ON396_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3113622.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            11727..12533
                     /locus_tag="ON396_001534"
     CDS             11727..12533
                     /locus_tag="ON396_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3113623.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            12669..13658
                     /locus_tag="ON396_001535"
     CDS             12669..13658
                     /locus_tag="ON396_001535"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCR3113624.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(13975..14499)
                     /locus_tag="ON396_001536"
     CDS             complement(13975..14499)
                     /locus_tag="ON396_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HCR3113625.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            14786..>15939
                     /gene="scm"
                     /locus_tag="ON396_001537"
     CDS             14786..>15939
                     /gene="scm"
                     /locus_tag="ON396_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCR3113626.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTST"
BASE COUNT         4618 a         3404 c         3073 g         4844 t
ORIGIN      
        1 aattaatagc ttcttaaaaa aatatggtct ctcatgatca tgcaagctat tcctaaattt
       61 tcaaaatcat agacaaaaaa gaatgtctat tgtactatgg aattattcta aaacgctccc
      121 aaaaaggagg tgcatataaa attgaaacct actatttatg acattgcgaa aaaagctaat
      181 gtctcaaaat caactgtctc cagagtatta aataaccagc caaatatctc tgataaagca
      241 aaagcaagtg tacttcaagc aatcaaagaa ttgaattatg aaccgagcaa aattgcaaga
      301 ggactgtcag gaaatggttt tgacgcgatt ctagcattat ctcatcgtac ttctcatacg
      361 acctatggca atcctttttt ttcagatgtt attcaatcca tttctgccgt ttctgaacaa
      421 aataacttcg acttgattct acaaacggca aaaacaccag aagaagagct agaaaaatgc
      481 gcgaaaaaga tccaagaaaa aatgatccga ggaattatta ttttaagctc tacgatggat
      541 gaaacacttc tttttgaatt ggatcaatac aatattccta ttgtggtaat cggaaaagtc
      601 ggaaacgact acacgaacat ctactcagtg gacacggata atttccagga tagttacgat
      661 ttggtggaaa cactgatcaa gaatggacac cgtcatatcg gctgtctcca tgctccgaca
      721 gaagtttatg tatccaatga tcgtctcaat ggttaccgtc aatgcttatt tgatcaccac
      781 ctagatatcc gtaatgaatg gattatagac ggcggctttc gcttagaaga tagcatggca
      841 gcaatgaatg atttaatgaa gctacgagaa agaccaactg cgattttttc taccgatgca
      901 ttaaaatcat tgagtattta caaagtggca gaacaatctg gaaagcagat acctgatgat
      961 ctttcgatcg caggattcag tgatccctta ttgtcctccc tattttcgcc acagttgact
     1021 cggatagaca ttccgacaaa tcagttagga atcaaagcca cagaactttt attccaacta
     1081 atccaccacc aataccctga agaaaaaaga atcattcttt caaccgagat tactctcaca
     1141 gattcgatcc aacaacaaga aatgtagaac agaattataa aagaacagaa acactgagga
     1201 taagtcagtt actctgttct tttttcaatc actcatccct cgttaccccc tcccggtatt
     1261 ttagcgaatt tttcttattt tccatctgtt ttagatagct gtggataact ataatgtttg
     1321 tgttaaacta ttttttccac agggaagaaa aaactatttt taaccacgtt gtgtataagt
     1381 ttttatagtt cgttttttta aacacattta cccttatttc aatccatgtt atttggggat
     1441 acttatctct aaaacataaa tagaataaca gacatgtcta ctggttttgt tttattcgag
     1501 gaattattca ataatagcta gcaaaatatt aaaaataacc gctctatctt cccatcaatt
     1561 tattttgcat tcaaaaaatg atctatcttt acttttgttg attgacttct cttggaagat
     1621 gatcgttccg ataccttaat tggatacgaa ccgatgttgt taagatcctt tcccactttt
     1681 cttctagtta gacaaattat tttagacaaa ccaaaaaaga ggttgtgaca ataatttgtc
     1741 acaacctctt ttttaaaata tctattttgt ttgattgttc attgcgtctt ctgcagccat
     1801 ttgcgcttca atatctgaaa tactgtagac attttcgctt gctacaccga cttcttttgg
     1861 ttccatgtca cgataacgtt gcataccagt accagctggg atgatcttac ctataataac
     1921 attttctttc aaaccaagaa gaggatcttt cttaccgcgg atcgcagcat ccgtcaagac
     1981 acgagttgtt tcttggaatg atgcagcaga taagaaacta tttgtttcta atgatgcttt
     2041 tgtgatacct agtaagacag gtcgagaagt tgcaggaacg cctcctgaga ctagcgtatt
     2101 gtagttttga tctttaaagt ctgcgatgtc taataatgta cctggtaaga tatctgtatc
     2161 acctggatcc attacacgaa ctttacgcag catttgacga accattactt cgatatgctt
     2221 gtcgccgatt tctacccctt gcatacggta aacacgttgt acttcacgaa gcaagtagtt
     2281 ttcaacagat aatacatcgc gaacttgcaa caattgtttt ggatcgattg atccttctgt
     2341 caatggtgca ccacggtgga tcatatcgcc ttctgctact ttcatacgtg ctgtataagg
     2401 aacagtatac gtacgtgtat ctgtttttcc tttgattgtt acttctttgg tacgtgttgc
     2461 aggatcttct gagatatcga tgacatcccc tgttacttct gtgatcactg cttgcccttt
     2521 cggattacgt gcttcaaaga tttcttggac acgaggaaga ccttgagtga tatcgtctcc
     2581 ggcaacccca cctgtatgga atgtacgcat cgtcaactga gtaccaggtt caccgattga
     2641 ttgagcggcg atcgtaccaa ctgcttctcc gacttcaacg tcagagccag ttgcaaggtt
     2701 gcgtccgtaa caatgtttgc agacaccatg ttttgtatta catgtgaaga cagaacggat
     2761 tgtcactgtt tcgacacctg catcaacgat ttgttttgcg atgtcttcgg aaatcaattg
     2821 gtttcctgca atgatcaatt catttgtttc aggatgacgt acttctttac gggtgtaacg
     2881 tcccactaaa cgatcttcta atgattcgat gatttcattt ccctcgcgga tggcttggat
     2941 ttcaagacct cggtcagtac cgcaatcttc ttcacggatg attacatctt gggccacgtc
     3001 aaccagacga cgagtcaagt aacctgaatc ggcagtcttc aaggctgtat cggtcatccc
     3061 tttacgagcc ccgtgagttg agataaacat ttccaagacg gatagtcctt cacggaagtt
     3121 cgaaatgatt ggcaattcca tgattcgacc attaggagcg gccatcaaac cacgcattcc
     3181 ggcaagctgt gtaaagttgg aaatgttacc acgagctcca gaatcagaca tcatgaagat
     3241 tggatttttc gcttccaaac cttccatcag tttttgttgg atgctgtctt ttgcagcatt
     3301 ccaaacagca atgacacgtt cataacgttc gtcatctgtg atcagaccac gacggaattg
     3361 ttttgtaata gtttcaactt gtttatgggc attttcaatg atttcttgtt tttcattcag
     3421 tacactgatg tcagcgatcc ctactgtgat accggcatgt gtcgaatgtt tgtaccctaa
     3481 gtctttcata cggtcaagca tcttagaagt ttcttttact ttgaatcgtt tgaagacttc
     3541 tgcgatgatg tttcctaagt ttttcttctt gaacggtaat actaattctt gctctttgat
     3601 atgtgcagga atatcagtac cagcttcaac aaagtatttg tccggtgttt ggacggtcaa
     3661 gttgaagtct gttggttcat tcagataagg gaattcagga ggcatgatct cgttgaagat
     3721 gatcttacca actgttgtga tcatcgtacg ttctttttgc cattcagtaa atggtttttc
     3781 acctaatgag ttagggttca caccgatacg tgaatgtaaa tgaacatatc cgttgcgcca
     3841 tgcgataacg gcttcgtttt gatctctgaa gaccattcct tcgccttcgc gtccttcttc
     3901 ttccattgtc aagtagtagt tacctagaac catatcttga gaaggagtaa caactggttt
     3961 accatcttta gggttcaaga tgttttgagc agctaacatc aacatacgtg cttctgcttg
     4021 tgcttcttca ttcaatggaa cgtggacagc catttggtct ccatcgaaat cggcattata
     4081 agcttcacat acaagtggat gcaaacggat tgcgcgtcct tgtactaaaa ctggttcgaa
     4141 tgcttggata ccaagtcggt gaagcgtagg tgcccggttc agcaatactg ggtgttcctt
     4201 gattacgtct tctaaaacat cccatacttc gtcttcttga cgttcgattt tacgtttcgc
     4261 atttttgatg tttgaagcta gttcgcgttg aacaagctca cgcatcacga atggtttgaa
     4321 taattcaatg gccatttctt ttggaagtcc acattggtac atcttcaaga aaggtccaac
     4381 aacgataacg gaacgacctg agtagtcgac acgtttacct agcaagtttt gacggaaacg
     4441 accttgtttc cctttcaaca tatgagaaag agatttcaat gggcggttac ctggtccagt
     4501 aactggacgt ccgcgacgac cgttatcgat caatgcatct actgcttctt gcagcatacg
     4561 tttttcattt tgaacgatga ttccaggtgc attcaagtct aacaaacgtt tcaaacggtt
     4621 attacggttg attacacgac ggtaaagatc attcaagtca cttgttgcaa aacgtccacc
     4681 ttctaattga accattgggc gtaaatctgg tggaataaca gggataacgt ccataaccat
     4741 ccagcttggt tgatttcctg aagcgcggaa tgcttctaaa atatccaaac ggcggattgc
     4801 acgtgtacgt ttttgtcctt gtgcagattt caattcttct ttcaattcgg ctgcttctgc
     4861 atctagatca acattatcaa gtaattgttt aatcgcttct gcacccatag cagcttggaa
     4921 ttcttgtcca tattgttcac gtttttcgcg atattcgcgt tctgtcaaca attgtttttt
     4981 ctctaaattc gtatttcctg gttcgatgac tacatatgaa gcaaaataaa tgatttcttc
     5041 caaagcgcgt gggctcatgt ctaatacaag acccatacgt gaaggaatcc ctttaaagta
     5101 ccagatatgt gaaacaggag ctgctaattc aatatgtccc atgcgttcac ggcgaacttt
     5161 cgaacgagta acttccactc cgcatcgatc acaaacgatt cctttataac ggatacgttt
     5221 gtattttccg caggcacatt cccagtcttt tgtaggacca aaaatccgct cacagaacaa
     5281 cccttcgcgt tcaggtttta aggtacgata gttaatcgtc tcaggttttt ttacttcacc
     5341 gtaagaccag cttctgattt tttcgggaga agccaaacct atttgcatac tttcgaattt
     5401 atttacatcg atcaaaaggg gttccctcca tttcatttaa tggcaaaccg caagacggct
     5461 gatcttcgac cgccttgcag ctcatagacc ggattagtct aaattatctt ctgcggtttc
     5521 aaagttttgg atgacatcgt ctttttcatc ttccacttgt tcagcagctt tcttttctaa
     5581 ttctttggca gtttgctgtt cggcaaattt tgtcaaagca tcaacagtga tcaaatcgtc
     5641 gtcttcatcg tccatgtcgc gaagttcgat ctctgtttct tcgatatcca agacacgcat
     5701 gtccagacct agtgattgca attctttcac caatacgcgg aatgattccg gtacacctgg
     5761 ttttggaatt ggttcaccct tgacgattgc ttcgtatgtt ttcacacgtc cgactacgtc
     5821 atctgatttg tatgtcaaga tttcttgtaa agtataagcc gcaccgtaag cttccagtgc
     5881 ccatacttcc atttcaccaa aacgttgtcc accaaattga gctttacctc ccaatggttg
     5941 ttgtgtaacc aatgagtaag gtccgattga acgtgcatgc aatttgtcat cgaccatgtg
     6001 agcaagtttg atcatgtaca tcacaccgac agacacacgt ccatcaaatg gttcacctgt
     6061 acgtccgtcg tacagaacag ttttcgcatc gctggccata ccggcttcac gaactgtttc
     6121 ccatacgtct tcatcacttg caccatcaaa tactggcgtt gctacgtgga tacctaactg
     6181 acgtgcagcc atacccaagt gtaattccaa tacttgcccg atgttcatac gtgaaggcac
     6241 ccctaaagga ttcagcatga tatcgattgg tgttccatca ggtaggaatg gcatatcttc
     6301 ttccggcata atacgggata caaccccttt atttccgtga cgaccggcca ttttatcccc
     6361 ttcatggatt ttacgttttt gaacgatata aacacgaact aacatgttta cgcctggtga
     6421 caattcatct ccagcttcac gagtaaagat tttcacatcg tgaacgatac cgccgccgcc
     6481 atgtggtaca cgtaatgatg tatcgcggac ttcacgagct ttttcgccaa agatcgcatg
     6541 caatagacgt tcttctgcag ataattctgt tacccctttt ggcgtgactt tccctacaag
     6601 caaatcgcca tcttggactt ctgcaccaat acggatgata cccatttcgt ctaaatcttt
     6661 taatgcgtct tccccgacat tagggatttc gcgtgtgatt tcttcaggtc ctaattttgt
     6721 atcgcgggct tctgattcat attcttcaat atggatagaa gtgtatacgt catcttttac
     6781 caaacgacgg ctcatgatga tcgcatcttc atagttgtac ccttcccaag tcatgaaacc
     6841 aactaaaacg ttttggccta gagccatttc gccttgttcc atagatggtc cgtctgccag
     6901 agtatcccct ttttcgactt tctcacccaa atgaacgatt ggacgttggt tgtagcttgt
     6961 tccggagttt gaacgacgga attttgttac atcgtattta tctaatgcac cgttgtcgcg
     7021 gcgtacgcgg atttcagaag catcaacgta ttcaacgaca ccatcatgtt tacataacaa
     7081 agcagctcct gagtcatgcg ctgatttgta ttccatacca gtaccaaccc atggtgcttg
     7141 aggattgatc aacggcactg cttgacgctg catgttggca cccatcaagg cacggttgga
     7201 gtcatcgttt tccaagaaag gaatacatgc tgtcgcaacg gcaactactt gtttaggtga
     7261 tacgtccatg tagtctactt tatcaataga tacttctaag ttttcacttt gagcacgagc
     7321 cataacaacg tcttgagcaa atgttccatc ttcattcaat gggctgtttg cttgggctac
     7381 gatgtaatgg tcttcgatat cagctgtcaa gtagtcgatt tggtcagtaa cacgacctgt
     7441 ttcacggtct acacgacgat aaggtgtttc gataaatccg aatttattta ctttcgcata
     7501 actggacaaa ctattgatca acccgatgtt tgggccttca ggagtttcga tcggacacat
     7561 acggccatag tgggagtagt gaacgtcacg aacttcataa ccggcgcggt cacgagtcaa
     7621 tccgccaggt cctaaggcga aaagacgacg tttgtgtgtc aattcgccaa gagggtttgt
     7681 ttgatccatg aactgtgaca actgagaaga accaaagaat tctttgatac ttgccacaac
     7741 tggacggata ttgatcaatt gttgaggtgt caatgtttca gtatcttgga tcgacattct
     7801 ttcacgaacc acacgttcca tacgtgctag cccgatacgg aattggtttt gtaaaagttc
     7861 accgactgaa cggatacgac gatttcctaa gtggtcgata tcatcgacat tgccgatacc
     7921 ttccatcaag ttcaagaaat agctcattga agcaatgatg tcggctggac gaaccgtttt
     7981 tacagctgct tctggatagc cgtttccgat cacgtttact tcacgttcag ggtctcttgg
     8041 tgaaaatact ttgatgactt ggacagtcat tggatctgtg accacaccgt cttctgatgg
     8101 atagtaagtc actgaattta ggccattatc aatgaaagga gctagtgttt ccatgacttg
     8161 gtgagtcaga actgttcctt tttcaacgat gatctcgcct gtttctggat caactaatgt
     8221 ttcagctaat gtcagattca atagacgtgt tttcaaatct aattttttgt ttactttgta
     8281 acgaccaacg tttgctaagt cgtaacgttt tggatcgaag aaacgagcat tcagcaagtt
     8341 acgtgagctg tctgctgttt ttggttcgcc tggacgcaaa cgttcgtaaa cgtctttcaa
     8401 accttcttct gttcttgagt cgcttgcgtt tttatgcaag tctttttcga ctgtattgcg
     8461 taatgtttcg ctgtcgccga agatttcaaa gatcgtatca tcggaaccaa atcctaacgc
     8521 acgaactaat acggttaaag gaatcttacg tgtacggtcg atacgtacat aagagatgtc
     8581 ttttgcatca gtttccattt ctagccaagc tccacggtta gggataacag tagaaccgaa
     8641 tccttctttt ccgtttttgt cgacttttcc atggaaatag acacctggtg aacggactaa
     8701 ctgagaaacg attactcgct ctgccccgtt gatgatgaat gttccttgtt ctgtcattaa
     8761 tgggaaatca ccgaagaata cttcttgtgc tttgatttcg ccagtctcgc ggtttgtcaa
     8821 acgcaatgtt acatgtaatg gtgcagagta gtttgcatca tgcgcacgag cttctgctac
     8881 tgtatacttt ggttctttca attcataatc tacaaattcc aaagatagat tgccattgaa
     8941 gtcatcgatt ggcaaaatgt cttcaaacat ttctctaagt ccctcatcta agaaccattg
     9001 gtaagagtct gtttgaattt cgatcaaatt tggtaattcc aacacttcac tgattcttgc
     9061 gaaacttcta cgttcgcgat gttttccgta ttttactacg tgtccagcca agctcttcac
     9121 ccctctaaaa tgtcattcaa aaagactacc cgatcaattg ttacaataat atttcaaata
     9181 ttatttttag gtaacaacag acattttttg cggcttttag gcaaaaaaaa accaaaaata
     9241 gaacattctc ttgaaacctt acttcgtgag cttctacttt tgtttcctta tattaaagcc
     9301 gaaacggaca atatttcgtt tctgcctttt cttcatcaga tagtttttgc aacttcatat
     9361 actacactta ttttctataa aagtcaacac ttcctggcgt ttcttctgct ttttcgcaac
     9421 attttcttaa tgttttcaat tttatgtttt caattatctg acaattctta attgacagac
     9481 ctttttatct cggttatact ttagaaaatt cattttgtgg aggaacttct atgcgaacca
     9541 acatcaacca aatcgcacca acacaactga ataactttta ctttagctac tttagatagg
     9601 tttgtattca tcatgatgga tacaaacgtt aaacgacagt ttgtatctat gatggctaaa
     9661 gaactttgta ttgcagcaaa aagccacgta gacaaaatgc taagtggctg agcagtgaaa
     9721 atacaaagta aaagtatcaa gcattctact ttttcaattt attttccgac ttttcaatcc
     9781 atttggacat tttgtctgaa tggatttttt gtttcccaaa attaaggagg aaaaagaaat
     9841 gaaaaaaaat ataatcatcg gattaggcgc tgccctgcta ctagctttag gaggttgcgc
     9901 acaaaaagac aattccgcag aggcatcaga tggacaaaaa acaattggcg tattgcaact
     9961 tgtacaacat aactcgttag atgctgccta taaaggattc aaagaaggat tagcagaaga
    10021 agggtataaa gaaggcgata atctaacaat cgattatcaa aatgcccaaa acaatcaaga
    10081 caacctgaaa agtatgagtg agaaactcgt caaagaagat cctgatttgc tacttggaat
    10141 cgccactcca gcagcacaat ctttattaaa tgaaacaacc actatcccga tgactgtcac
    10201 agcggtcaca gacttggaac aagcccattt agtcacatcc aatgaaaaac ctggcggcaa
    10261 tgtgactggc acgacagata tggtgccaat cgaaaaacaa atcggcttac tgttaaatat
    10321 cgtgccagac gccaaaacaa tcggcatcat gtataacaac ggtgaagcaa actctaagat
    10381 ccaagctgat ttggcagaaa aagaattgaa aaaagcgggc gtcaaagtca aagtactgac
    10441 agcaaattcc acaaatgatg tccaacaagt gacgaaaagt ctagctaaag atgtagatgg
    10501 aatctatatt cctactgata acacgttcgc aagtgcggca agtgttgtcg gtgaagtcgc
    10561 aaaagaaaca aaaacacctg tcgttgctgg cgcagttgat caagtcaaag acggcggtct
    10621 agcaacagta ggaatcgact acgaaagctt gggcaaacaa acagggatca tggcagcaaa
    10681 aatcttagac ggcaagaatc caagtgaact tcctgtcgaa acagcgaatg atttggaact
    10741 gtatgtcaat aaagacatgg ccaaagcatt aggcatcaat ccagacagca tcaaagaagc
    10801 tgaataaaac acaggaggat tctcatgaca aatttgctct ctcttttctt atcatctgca
    10861 tcgcaagggg tcttatgggc cctacttgcg attggtgtat acgtcacttt ccgtatcttg
    10921 gacattgccg acttgactgc tgaaggaagc tttcctttag gtgcagggat cgcggcagta
    10981 agtattacaa atggctatca tccattgaca gcttgtcttc taggcttttt agggagtgcg
    11041 gcagcagggc ttgtttccgg actgcttcac acaaaattga aaattcccgc ccttcttgca
    11101 gggatcatca ccatgactgg tttatattct gtcacatccc gtgtgatggg tgcgccgaac
    11161 atctctttat tgggtcagcc aacgatcttt cattgggtcg agtcattcgg cataacaaaa
    11221 gacaacgctg tactgatcgt cggtctgctt tttgctttga tcgttgtatg tttacttgtt
    11281 ttgttcttac gtacagaaac aggacttgct attcgtgcaa ctggcgataa tctggcaatg
    11341 agcgaagcaa acgggatcaa cacagataat atgaagatca tcggttatat gatttctaat
    11401 ggatgcattg ctctttctgg ctccttgctt gcgcaaaaca acggctttgc tgatttaaat
    11461 tctggtattg gaacgatcgt aattggactt gcttctatta tcatcgcaga agttttgtta
    11521 cgtaaccagc cactatttct ccgtctcttg actgtcgttt taggtgcggt catttatcgt
    11581 tttattttag cgttagtctt cgaactgaat gttgaaccaa gtgattcgaa acttgcttct
    11641 gctttcgtgc tagtgatctg tctttctttc ccgcagatcc agcaaaaatt ccatttacct
    11701 caaaaaaaga aaggcggtag tgtcaaatga atgacgtctt aacattggaa aatctccatc
    11761 atacctttga aaaaggaacg atcaacgaaa atcatgtcct gaaaggcatt aatctttcct
    11821 tagatcccgg cgacttcgtg acgatcatcg gtggaaatgg ggctggtaaa tctactttgc
    11881 tgaacagcat cgccggtaca ttttctgtcg atcagggtca aattttattg aatggaaaaa
    11941 atatcacgaa aaaatctgtt gtcgaacgtt cgaaatccat cagtcgcgta ttccaagatc
    12001 ctaaactagg gacagccgtt cgtttaacgg tagaagaaaa tcttgctctt gctatgaaac
    12061 gaggaaaaaa acgcggcttc ttccgtggtg tcaaaccaca agaccgaaca tttttcaaag
    12121 accagctggt tcggctaaat ttggggttgg aaaatcgttt atcctcagaa atcggtttgc
    12181 tgtctggagg acaacgtcaa gctattacgt tattgatggc cacattacag cgtccagaac
    12241 tgatcttatt agatgaacac actgcagcgc ttgaccctgc tacttccaaa acggtgatgg
    12301 aattgacaga aaagctgatc actgagcaga atctcactgc ttttatggtc actcatgaca
    12361 tggaagacgc gatccgctat gggagtcgac tgatcatgct gcatcaagga aggatcgtcg
    12421 tagatatcca gcaagaggaa aaacagcagc tcacagtgcc ccaattatta gcgttgttca
    12481 aacaaaacag cggcacggca ttaaaagacg atcaagtact tttatcttca taatcagaaa
    12541 gaggatggga caaaaaatga acgcccaccc tcttcttttt tctctttcct tctttcgttt
    12601 ttgctttatg tatcaaccgt tgtttcttcg aaaactattt tgttctctcc ttcttcctga
    12661 tagaatggat gggaaaggag cttgatgcca tgacaacgaa agacaaggta cttcaattat
    12721 tgaaaaagac accagatttt ctttccggag aaaagttggc acagcaactg gaagtttccc
    12781 ggacaagtat ttggaaagcc atcaaagaat tggaaaaggc aggctatcgt tttgagcacg
    12841 ggcctacagg gtatcgttac ctgccctctg acgtactaga tgcaaaagaa atccagcaag
    12901 agctacggtc gtttattcct gatttgaaag tacaaacgat gatgacttct gaatctacga
    12961 tgaaagatgc caaactggca gcagctcaag gagattctgt ttcgaagtta tttgttgctg
    13021 atacacaaga acaagctcgt ggacgttttg gacggccttt tttcgctgaa cctggacgag
    13081 gaatctacat gagtctcttg ctccatccag acaaacggtt tgatgaattg cctcaatata
    13141 cgatcatcgc agccagtgcc tgtgtgaaag ccattgaatc tgtaaccggt cgttctgctt
    13201 cgatcaaatg ggtcaatgat atctacatgg atggaaaaaa agtctgtggg attttatctg
    13261 aagcagtcag tgatatggaa agcggcagga tcagtcatat catcattggg atcgggctta
    13321 atttttctgt tcctcaaaat cattttccag ctgatctcca agaaaaagcc atctctgtct
    13381 ttcccgacgg acaagctagt gtcacaagga atcagctaat cagcaaaatc tggcaagaat
    13441 tgttcactta tctttctcac ccagaaaaag cgttggactt ctataaagaa aaatcattcg
    13501 tattgggaaa aaccgtgtcg ttcaatcaaa aaggaacgac ttatgtcggt cttgcaaaag
    13561 aaatcggtga tcatggcgaa ctgatcgtcc aaacaaatga acagaagatg agtttgtctt
    13621 ctggcgaaat cagtctcaca tctatccgta cgcaataatt atcagatttc acaaaaggac
    13681 acctgatctc atacagtgtc cttttttctg aattctcttc aaaagactct ccttattcta
    13741 tcagctgaca gtttctctat cctctagaaa aaggtattct aaaaatatct ataaagcatg
    13801 gtacactttc tttaaatgaa tcaatgcaaa aaacaagaga gtcatcagtt tacacgatca
    13861 aaagagatag caggccgctt cctttttcaa tcttatagat agaccatggg taacgataaa
    13921 aacaaaaaag aacttgcgac ttattttgcc gtaagttctt ttttagctag tgccttaatc
    13981 tcttaatccc aacaaacgtc tcgcatagat cacctgacca gagtgcatag cagcatcatc
    14041 aataatagaa acgagacgca ttcctcgtgt gactggtggt gtccagttct cgtcaattac
    14101 ttcatctagc tcggaaggtt ccagccttct taagtaagaa atagcaaaat cagcagctcc
    14161 tcgcaagtat cctagcagat cttctatttg attagcttgg atctgctttg cttgttccag
    14221 actatgcttc caatcttgtt cgttatctgc tatcgtcatc ggaaaccggc tttcccagtc
    14281 ttgactatgc caaattggag aagttcctgc taaatcagca atctggaaat ctaattctcg
    14341 agcggtatgc caagccagcc aagtcattga tttgatctgc ggggctctat cttccactgg
    14401 aaaacgattg gcctcctcaa tagagacgcc ttcaaacaaa cgttcaaacc gatcgtatgc
    14461 ccgatccaca ctctcgcata gcatatctaa ataatccatt tattttcctc ctttttattt
    14521 atcctactta ctttttccta attctaggat acctacttat atgttactac acacccttat
    14581 tttttacact ttaacagtca ccatcatcaa tagtttggag attgaaagaa aaagatagtg
    14641 tcgaactatc aattgaacaa gaagcaacaa tatgaacgtt tcattaatat tctttaaata
    14701 aacaatgatt tttcaaacga acagtttaag actaggagaa aaagagtata ataggaaaat
    14761 gtttgaaagg aaaggaaggg gaaaaatgaa aaaaagggca agtttactag tcctagttgc
    14821 tttgttgttc aatatctttt cgtatggtgt ggccgtttca gcggaaacat acattcctaa
    14881 agaccctggg aatatttata atgatctaac tggtaactat ggcttgttag gaattgctag
    14941 tcaattccac gtatttgcta aaaataagac aactgtgaat gcacatacag acggaaatat
    15001 agcaacacat gaacttgatg caaaaaataa ttttggtaca gagcttcatg aaggtttatt
    15061 gaaccaagaa ataaattata tacaaaaaac tgattctttc gctaattctt caggcatacc
    15121 tacaggtagc acaagaatga ataaatttgt cgttggcagt aacgtggatg tagcacttca
    15181 agatggtcat cctacagtca atggatccag gcttgatcac ttaactccag atgattttta
    15241 taaagatcgt gctgggcatg aatatattga tttcgagcag gaatttgcta aattgaatgt
    15301 agcagcaaat gatcttgcaa cgatcactcc agctaaaaca tatactgctg cagattttcc
    15361 agacatgaac aatcggacaa tcgatttaac tgatttatca gataccgggt ttttattggt
    15421 aaatatcgat gcagaagtgt taacgatgaa tacgccactt caaatcatta atccaaatga
    15481 tcaagtagtt gtatttaatg tcatcaacag ttcttctgca ttgaacgtac agtcaccaat
    15541 caaatacaat gatcgttcaa atcatgaaac agaagatttt tcggatgcga atatctcttg
    15601 gaattttgga aatgagatga ctgatttgac aatcagtgct ccttttcaag gaacaatcct
    15661 tgcgcctaat gctactatcc gcattactca aaaccaagac ggaacgatta taggaacaaa
    15721 tgtgatttta gatgcagcta ccaatcgttg ggatcctaac gaaatattta tcagcgacaa
    15781 tggtacagat acgacagaca caagcgacag tacagataca tctaccacaa gtgatagctc
    15841 agattcatca acttcaagcg acagtacaga tactacctct accacaagtg atagcacgga
    15901 tacatcagct tcaagcgaca gtacagatac tacctctac
//
LOCUS       DAJSKS010000058        15520 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJSKS010000058 DAJSKS010000000
VERSION     DAJSKS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.059"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            82..564
                     /locus_tag="ON396_001538"
     CDS             82..564
                     /locus_tag="ON396_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113627.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            761..1651
                     /locus_tag="ON396_001539"
     CDS             761..1651
                     /locus_tag="ON396_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HCR3113628.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            1976..4447
                     /locus_tag="ON396_001540"
     CDS             1976..4447
                     /locus_tag="ON396_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR3113629.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(4550..6286)
                     /locus_tag="ON396_001541"
     CDS             complement(4550..6286)
                     /locus_tag="ON396_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCR3113630.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6283..6987)
                     /locus_tag="ON396_001542"
     CDS             complement(6283..6987)
                     /locus_tag="ON396_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3113631.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7118..7621
                     /locus_tag="ON396_001543"
     CDS             7118..7621
                     /locus_tag="ON396_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HCR3113632.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7581..7919
                     /locus_tag="ON396_001544"
     CDS             7581..7919
                     /locus_tag="ON396_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCR3113633.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7940..9358
                     /gene="ffh"
                     /locus_tag="ON396_001545"
     CDS             7940..9358
                     /gene="ffh"
                     /locus_tag="ON396_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR3113634.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9470..10048
                     /locus_tag="ON396_001546"
     CDS             9470..10048
                     /locus_tag="ON396_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR3113635.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10052..10573
                     /locus_tag="ON396_001547"
     CDS             10052..10573
                     /locus_tag="ON396_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3113636.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10652..11206)
                     /locus_tag="ON396_001548"
     CDS             complement(10652..11206)
                     /locus_tag="ON396_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR3113637.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11459..11734
                     /gene="rpsP"
                     /locus_tag="ON396_001549"
     CDS             11459..11734
                     /gene="rpsP"
                     /locus_tag="ON396_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR3113638.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11746..11997
                     /locus_tag="ON396_001550"
     CDS             11746..11997
                     /locus_tag="ON396_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HCR3113639.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12122..12913
                     /locus_tag="ON396_001551"
     CDS             12122..12913
                     /locus_tag="ON396_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113640.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13083..13604
                     /gene="rimM"
                     /locus_tag="ON396_001552"
     CDS             13083..13604
                     /gene="rimM"
                     /locus_tag="ON396_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR3113641.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13594..14355
                     /gene="trmD"
                     /locus_tag="ON396_001553"
     CDS             13594..14355
                     /gene="trmD"
                     /locus_tag="ON396_001553"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR3113642.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14491..14838
                     /gene="rplS"
                     /locus_tag="ON396_001554"
     CDS             14491..14838
                     /gene="rplS"
                     /locus_tag="ON396_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR3113643.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(14970..>15520)
                     /locus_tag="ON396_001555"
     CDS             complement(14970..>15520)
                     /locus_tag="ON396_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3113644.1"
                     /translation="SHNKRYSLLFQWLYLTGMRSGEAIALSKDDVHITNNNASVVING
                     TMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNPNGQFLFQTTKGTP
                     FQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKLAELGTPLYAIQDRVGHENSDIT
                     EKIYLHVTKGVKEKLKEDIEKL"
BASE COUNT         5104 a         2810 c         3189 g         4417 t
ORIGIN      
        1 aaaaaaagtt tttcccccta atttttttag atagaaaatt gtatagtatg atgagagaaa
       61 aagtagtttg ggggaacgtt aatgagaaga aaagcagtaa tcgatcggct ttatttattt
      121 ttttggtttt ttcctatcgt tttgctacct tttacatggg taaatgctgt tgtacaaggt
      181 gcggagatat taggcctgat ccaaggaagc ggatttgtgg tttctaggaa ttttccagct
      241 ttgactgttt tctatttagt gataagtgta ggtctcttat taccaaaatc tctgatcaat
      301 cactacaatt ggcagctgct ttacttttct tgcttattag tatttacatc tatgttttct
      361 ttgctgcctc gcttgattat cggccctaat ggtttgagcg agagtggatt gaaggattca
      421 gcttattcct ccatgtattt cttgacgatc aaaccatggt tctatttagt aattgcctca
      481 tttattcttg caacattttt ttacttctgg acagaaatca ggataaaaag tgaacgatct
      541 gtatctataa aaaataacta ttgatggtgg atcatcagta aaaaagcata tgagctgcgt
      601 aaaatgcatg tttctcatat gcttttttta ttattatgta aagaatgcga aaaacgttta
      661 catgattttt acataactta actatatttt tacagcaacc atacgaggga tttacagtcg
      721 actgataaac tttagttgta aataagaaag gagcaataaa atgaaaaaag tattgtttac
      781 tctaggtatg atgggaattc ttttagccgg atgcggctca ggatcaggaa atacagatgg
      841 ctctgccaca caaaataatt caagtgactc aaatgaacaa gtaaaaatcg ttgcggtagg
      901 ttcgacagca ttacagccgt tagtcgatgc agcacaagaa agctttgtac aagaaaatcc
      961 aaattatcaa atctctgtac aaggaggagg aagcggaact ggtttgagcc aagtcgaagc
     1021 tggagcagtt actatcggaa actctgatgt atttgcagaa gaaagagacg gagttgatgc
     1081 ttcaaaatta gtggatcata aagtagcagt ggtcgggatg gcgccaatcg tcaataaaga
     1141 tacagatgtc aaagatatca ccaaacagga attgattgat atctttacag gaaaaatcac
     1201 taactggaag gaagttggcg ggaaagatca aaaaatcaac gtcgtgaacc gtgcaaacgg
     1261 cagcggtacc cgcgcgacat tcgaaaaatg gggactggat ggtgctaccc ctgtgcagtc
     1321 ccaagaacaa gattcatcag gaacagttcg gcagctggta agtcaaacac caggagcaat
     1381 cagctattta gctttctcat atctcgatga ttccacacaa gcactaagta tcgacggtgt
     1441 agaaccaaaa gaagagaatg tagcagataa cagctgggga atctggtcat atgaacatat
     1501 gtacacgaac ggaaaacctt cccctgaagt ccaaaaattc ttagactaca tgatgacaga
     1561 agaaatccaa gaaggacctg tcaaagaatt gggctattta ccaatcacaa tgatggaagt
     1621 agaacgtgat cacgaaggaa atatcaaata actagcagat aaacatgcca cggtagtcgt
     1681 tactattaag gaaaaatcaa ctatgtttcc cacatataca tttttccttt aattgtgaaa
     1741 aatagttcct catattttgc cgttttttga gctaataccg agggtcagct gattaaacac
     1801 cgtttattcg aaaaaaagag ctacgacata gaactgtcgt agctcttttt ttgctatatg
     1861 acaggaatga aaggtgtttg caagcttaat gtgaggaaca ctttccatat ctgttctata
     1921 ctggatagga gaagtttgga aatatccaga aaacgaaatc aatcgaggta gccacatgaa
     1981 gaataaatgg ataaatcgaa gtaaaaaaaa gaaagtaaca caaagatcat cagataaagt
     2041 catccgtctt tttccatcgt ataaagaagg attaacggca gatcaagtca aggaacgaat
     2101 agaaaaaggt gcagcaaata attcagtcga tcccacattc aaaacaaatc agcaaatcgt
     2161 attggaaaac atatttacgt atttcaattt gatctttctt atcttagcca ttttgctttg
     2221 tttagtagaa tcctataaga acttgacttt tttaccagtt atcatagcca atacaggaat
     2281 cgccattttt caggaaattc gatccaaaaa gatcttggac gaactaaatg ttttaaatac
     2341 agcaaaagtc aatgtcatac gggacgggat agaacaaaca atagatatag aagaccttgt
     2401 aatagatgac attgttattt tagagactgg acatcaaatt ccagcagatg cagaggtcgt
     2461 ggaaggaaaa ctgcaagtta atgaggcttt attgacaggc gaagcagatg aaataaccaa
     2521 agaaataggc gatccattaa tgtccggcag tttcattgtt tccggaaaag cttacgcccg
     2581 attagacaaa gttggagcag attcgtatat ttcccagctg acagtcaaag ccaagacaat
     2641 gggagaagga gaacagtcgg agatgattgc ttctcttaat caattgatca aatggattgg
     2701 gatcatcatt attcctatcg gtatcctgct tttttctcaa agttactttt ttaatggaaa
     2761 cacgatcaaa gaaagtgtcg ttgcaatgga agcagcattg atcggtatga tacctgaagg
     2821 actgtacttg ctgacgacga ttgcattagc attaagtgcg actaaactag caaagcaaag
     2881 agttctttta cacaatatga aaagcattga gacattggca agggtaaatg tcctttgtgt
     2941 agataaaaca ggaacgatca cagaaaataa aatgtctgtt caaaaagtga tcgtctctaa
     3001 aaaacaagaa ataatcagtc agtcagatca attggaagca tgcattgctg actatgcaaa
     3061 agcgatggca ggagacaatg caacaatgga agctgttaaa gcatatttta caaaaaccac
     3121 tgatcaatcc tatcaccatg ttattccttt ttcttctgtc caaaaattca gcagtgtagc
     3181 gttggcagac aaggtgtatg ttttaggagc gcccgaaatg gttttgcgcg atcaattgcc
     3241 ggaatatgcg gaagagtttg tttctttcgc tgaaaaaggc tatcgcgtgt tggtctttgg
     3301 tgtgtacgat gggattcttg aagaaccagt attgacagaa gcagtcacac cattaggcta
     3361 tattctgatt gctaatccta tcagaaaaga agcaagagcc acatttgatt acttcaaaaa
     3421 acagcaagta gccatcaaag tgatttctgg agataatccg ctcaccgtat ccaatgtagc
     3481 tgtccaagca gggatttcac atgctgatca gtacgttgat gcatctaaat tatcagaaga
     3541 agaatatgaa gcagctgtcg aaaaatatac agtttttgga cgcgtcaagc cagaacaaaa
     3601 gaaaatcttc gttcaattga taaagcgtaa caatacagta gctatgacag gtgatggcgt
     3661 gaatgatatt ctagccatga aagaagcaga ctgcagtata gcgatggctt ccggaaatga
     3721 agccgccatg caggcctcac aagtggtctt acttgattct gacttttcac gcatgccaga
     3781 agttgttgca gaaggacggc gcgttgtgaa caatattgag cgatcagcca gcttgttttt
     3841 agtgaagaat attttctcct ttttgctctc actgttttca gtgatctttg ctctgaccta
     3901 tcctttagaa ccatcacaaa tcacattgat tagtttgttt actatcggac ttccatcttt
     3961 cttgcttgct ctagaagaaa acaaaaagcg aatcagaggg aaattcatca tgaatgtgat
     4021 ggaaaaagct gtgccaggtg gactaacaga tatgatcgta gtcggtgcgt tggtgatctg
     4081 cggggtgact ttgaatctaa ataaaacaga tgtttctaca gcatctacca tgcttttgat
     4141 tgcagtaggg ttcctcgttc tctataaaat ctgttcaccg ctgaataaat tcagaagtca
     4201 gatcattctt ttctgtgcta gcggtaactt tttttcggtc atctttttgc ataagctgtt
     4261 ttcaatcacc ggtatctcag ctgtatcact tttgctgttg agcattctat ttttctcggc
     4321 agattcgatt tttaggcatt tgaccacatt ggtagaatat ttgtttgaac gtaaaaatga
     4381 aaaagagcaa aagccgttta gtcgcgtagc taaaaaattt ctatcgatat ttaaacgaga
     4441 aaattgacga aaagtatctc aaaaaagagt aaaaaagaac aaccactcgt gtatctttcg
     4501 aagcaggatg agtcatccaa ctttaaaacg cacgagcagt tgctcttttt tacattttcg
     4561 gaatggtaac ggtgaaagtc gaacctgttc caagatgact gtctacagta atcgtaccat
     4621 taaggagttg cacgtagtct tttacaatcg ctaaccctaa tccggtcccg cctgaatgtc
     4681 ggcttcgcgc cttgtctacg cggtaaaaac gctcaaagat ccgttcctga tcctcaatgt
     4741 cgatacctat accaaagtct tggacagaaa acgatagatc atctgttgcc tgatagcgga
     4801 taataatctc gctgtcgctt tttgaatatt ggacagcatt ttcaatcaga tttttgataa
     4861 ttggataaag gatatctgtt ttagtagtaa atggttcgtt ctttccaatc aatcgaatcg
     4921 tcaaccgttt cttttcgatg attggctgat aattttgaac gatttgctgg aaataaggtt
     4981 ctgtataaat tgtctggatc tcgtaagaat tctcgccatc ttttgacagc tggatgattt
     5041 cccgaatcag tttatctaac cggatcgcat ctttttgcat gatctcaaga aaagaagtca
     5101 gagtttgcgg gtcatctttt gcaccatcca gtaaagtctc ggtaaaacca atcagtgaag
     5161 tcacaggagt ttttaattcg tgagagacgt tgccaacgaa atccttttgt agcttttcta
     5221 aacgtcgcac tttcgttaag tcatacgcca cccctagaat ctgtccggta ccttctgaat
     5281 tagtaaagta gcgcaaactc aagtctaatg tctgccgacc ttctgtaagg gtgatctcct
     5341 gatggatcag cggatgctca ggagtcactt gatgaatcag ctgaatgagt tttggctctt
     5401 gaatgacttc tgtgtaccgc tgttcaggtt gatagcttac cacgtttaaa tgtagctgca
     5461 tctttggatt aagcagtagt aaacgagaat cagcatcatc aatcaggaat acgccaatca
     5521 tcagttcgtt cagtaacgta tataattgct cttctgtggt agtacacgcc cgatacatct
     5581 tactcatttg ttcgctcaac gcattaatcg tctgatacaa ttcttcccat tgatcgggtg
     5641 tttgcatgat tacttccgcc tgattaggat tttgcaccat tttttttagt acgggcagca
     5701 cggttttcaa tggctggttt ttttgataga tcagataata gatcattcct gcaagaacaa
     5761 ggaagaaaga cagaaagaag aaaaagaccc aacggcgaaa actctcagtt ctcggtaaaa
     5821 aaccgcttgt cggttctgcg atacgtaaaa tggcttctaa ctggccactt tttttgactg
     5881 gtaatgcgac atataaaaga tcctcattca aagtagtact tttgcgcaaa gcgctaccta
     5941 ggttcccacc attcaatacg gctttgattt cgggacggga gtttcgctgt tcattcaatg
     6001 tttcattgtt tgtgtcaaat aagatctttc cagtagcatc cattaaagta atccgttcgt
     6061 tcgactgatg gacgaattct tccaaagcag ttttgttttg cgctgcttgg aggttttcga
     6121 catctaattg ccggatcagc agttgtgctt tttttgtcag gtagctttct tgctgttcca
     6181 cgatctgctg ttgaaaataa tgggaaatca attgccagcc accaaaaaac aaactaacta
     6241 aaagaataag aaagaagaga cgttcataaa tttttttcca tttcatcgtt ttggttcctg
     6301 aaacttatag ccaaatcctc taatagtcac caagtacttt ggatgttttg gatctcgctc
     6361 gattttttct cgcaaatgac taacgtgtac atctacgatt cggctttgtc ctgcgaagtc
     6421 aaaattccaa atgcggtcta gcaatgtgtc tcggtcgatc acacggtctt ttcgtttcat
     6481 gaaataaaca agtaattcaa attctttcgg tgttagttca atcagatggt cgtctacggt
     6541 cacttgataa ttggtcaaat cagcagtgat ttgtccaatc gatagatatt caggctcagc
     6601 ttcatctttt gattgcttac gaggttcaat ccgtctgaat attgctttca tacgggctaa
     6661 tacttcgcga ggactaaatg gtttggtcaa atagtcgtca gcacctattt ctaggccaat
     6721 gatccgatcc acttggtcat ctttagccgt cagcatcaaa atcggtgttt catttttttc
     6781 ttgacgcagc cgctgagtga tctccatgcc atccatcgtt ggaagcatca catctaaaat
     6841 gatgaaatca aatgattgtt ctaatgctaa ttctaatcct tcgcctccat ctgtggcact
     6901 ggttaccttg tagccttctt tttccaaatt gaacgtcaag agcgttacaa tagaaggttc
     6961 atcatcaaca actagtattt ttttcattca aatctccttt aaataggttt cattctgttt
     7021 atattttagc atagaatcag tacaaagggc tattttacaa aagaaataaa tggtataata
     7081 ggaaggcact ttgctgaata gaaaggaaga gcagtcaatg attggaatca gtgcatgttt
     7141 aggcggtctg gcctgtcgct atgatggaca gaaaaaagaa gtaccagaat tgatggaact
     7201 agttgaaact ggagaagctg tcatggtctg tccagaagtg atcggcggat taccaattcc
     7261 tagagatccc gctgaaatca ttggtggaaa tggattcgat gtgtggaaag atcaagcaaa
     7321 agtatggacg acaagcggcg aagatgtaac ggaagcgtac aaaaatggcg cgatcaaagc
     7381 ctatcaaaaa ttgcaggaaa aacacattga tctagtcatc atgaaggaaa aaagcccttc
     7441 atgcggaacc cattcgattt atgacggaag tttttccgga ataaaaaaaa atggtgccgg
     7501 agtggctact gcttatttta ttcagcaaaa aatgactgtg ctctctgatg aagaatggct
     7561 gaaaactaga ggtgagcaga atggaaatcg aaaagaccaa tcgaatgaac gcgctctttg
     7621 aattttattc gaccttattg acagaaaaac aaatgaacta catggaatta tattatgcag
     7681 atgatttttc attaggtgag atcgcggaag aatacgaagt aagccgacaa gcagtatatg
     7741 acaatatcaa gcggacaagc aagatcttgg aggattatga aaaaaaactc catctttttt
     7801 ctaattatat tgttcgagaa caggtattgg aaaatatgac tgcatatatt acagataagt
     7861 acccggaaga taaaaaacta cttgaatatg tacagcaaat tcaagcaatc gaagaataaa
     7921 taaaaggaat gaagaaaata tggcatttga aagcttaact gaacgtctcc agcaggcgat
     7981 gggaaaaata agaaaaaaag gaaaagtatc agaagctgac gtaaaagaaa tgatgcggga
     8041 aatccgcctt gccttactag aagccgatgt taacttgcaa gtagtcaaag attttactaa
     8101 gcgcgtacgc gaacgtgcgg taggggcaga agtgctggaa agtttatcac ctgcacaaca
     8161 aatcgtaaaa atcgttgatg aagaattgac gatcacactt ggttctgaaa cagcagagtt
     8221 aaataaatca ccaaaaatac caactgttat catgatggct ggtcttcaag gggcaggtaa
     8281 aacgaccttt actggtaaat tagccaatta tttaaagaaa aatgaaaatg ctcgtccgct
     8341 cctaatcgca ggggacgtat atcgaccagc tgcgatcgac caattgaaag tattaggtca
     8401 acagctagac gttcctgtat ttgatatggg aactgaagtc agcccggtag agatcgtccg
     8461 tcaagggatg gagttagcaa aagaaaagaa aaacgactat gtattgatcg atacggcagg
     8521 acgtctgcat atcgatgaaa cattgatgga tgagttgaaa caaatcaaag aattgacgca
     8581 accaaatgag attttgcttg ttgtcgatgc catgactggt caagatgccg tcaacgtagc
     8641 tgatagcttc aaccagcaat taggtattac aggggtcgtc atcactaaac ttgatgggga
     8701 tactcgaggc ggggcagcac tatcgatccg ctcagtgaca ggcgcaccaa tcaaattcat
     8761 cggttctggt gaaaaattga ctgacttaga agtattccac ccagatcgta tggctagccg
     8821 tatccttggt atgggggata tgctgacatt gattgaaaaa gcgcagcaag actacgacga
     8881 gaaaaaagcg gaagaacttg cacagaaaat gcgggaaaat tcattcgatt tcaatgactt
     8941 tattgaacag cttgatcaag tgatgggtat gggcccactg gaagatctaa tcaaaatgat
     9001 cccaggaatg aatcaagtcc ctggaatcga aaacgtcaag gtagatccaa aagatgttga
     9061 acgcaaaaaa gcgatggttt attctatgac gccagcagaa agagcaaatc ctgatctgtt
     9121 gaacccaagc cgtcgtcgcc gaattgctgc cggttctgga aacagtgttg ttgaagtgaa
     9181 tcgaatgatc aaacaattca aggaatcaag aaaaatgatg cagcaaatgt ccaaagggga
     9241 tatgaacatc ccaggtatgg accaaatgtt tggttcaggc gtcaaaggaa aactaggcaa
     9301 aatggcgatg aatcgaatga tcaagaaaaa taagaagaaa aagaaaaaac ggaaataaga
     9361 tgaaaaaaac agcttcggta cagactgaaa atgtctactg ccggagcttt tctatcagca
     9421 atttttattc tttactaata caattactct aaacgaagag gtggcacaga tgagatgtcc
     9481 gtggagtgaa caaacagaaa gtatgcggca atatcatgat actgtctggg gtgttcctga
     9541 atatgatgac caaaagctgt tccgaaaatt gatgcttgat attaatcaag ccggattgag
     9601 ctggcagacg attttaaata aaagtgaagc attcgatgaa gcatatgatc gattcgatat
     9661 caaaaaagtc gcaaattatg gagaaacaaa aatcagtgaa ctaatggaaa acaaaggaat
     9721 tattcgtaac cgaagaaaaa tcgaagcagc cgtccaaaac gccaaagcag tcttgaaaat
     9781 tcaagaagaa atcggcagtt tttcagagta tctttggtcg ttttctaatg gaaaaatcgt
     9841 tgattcttct tatcattcac aagaagaagt cccaacaaca agtcctcttt cagatgaatt
     9901 agcaaaagat ctaaagaaaa gaggatttaa atttatcgga agcacaacaa tctatgcttt
     9961 tttagaagct gtcgggatca tcaatgatca ccttgacagt tgtttccgca aagaagaggt
    10021 cgtacgtatc ggcagaaagg gcgaataaag catgcgaaaa atcatatttt atggtgctat
    10081 aagtttagat ggctatttgg caacgaaaac ggatgatctt caatggcttt ttgacactcc
    10141 aacaggtaag caaactactt atgaagcttt ttatgaaaca atcgatacga ccatcatggg
    10201 cagaaaaact tatcaggaag caaaaaaata tttggagacc gaagcaattt atccagagaa
    10261 aaagaatttc gttttttcca cccacgcaca attacaattg ccagatgctg aggtcgtaaa
    10321 cgaagatcct gttgcttttc taaaaaatct acaaatgaca gaaggaaaag atatctgggt
    10381 tgtgggtggc ggtcgattga tcaaaccgct attggaacat caaatgattg atgaatggta
    10441 tatccagatt acccctgtat tattagggga cgggattcct ttattccaaa aaggaacata
    10501 tgaagcgcgt tttgaaatgc tagacacgac acgattcggt gaatttatcg aattgcatta
    10561 tgtacgaaag tagaagtaaa taaaataaaa aaggcaacta tatgcagaaa tgtcctgtac
    10621 atagttgcct tttttattac cgttatgctt tttactctgt taatttgtta gaaaaatcta
    10681 gatacgcatc taaaatcgtt tggaagaaac ttaatgttcc ttcaacgata ttaccatttt
    10741 catcaattag attcgcaacg tttccgatat atgcttccgg ttgctgcaag gttggcacat
    10801 tcaaaaagac aagtgattgg cgtagatggt ggtttgcgcc aaagccgccg atcccacctg
    10861 gtgatgcagt aacgactaaa cctggtttat gatcccaaac actgtgtcca taaggtcttg
    10921 agcctacatc taaagcattt ttcaatactg ctggcacaga acggttgtat tcaggagtaa
    10981 cgaaaacgac accgtctagt cccctcactt cctcacgaaa acgagtccat tcagcaggag
    11041 cctgttctgg agtttcgaga tcctcattgt aaaatggcag atcatcgatt tttacaaata
    11101 cactttcgta cccttcagga agcaatttcg caaaagcttc tgctactttt ttgttgtaag
    11161 aatcttttct taaactacca ataaaaaatc caattttttt tgtcatacga tttttcctcc
    11221 ttcaaactat ctataataat cttacaaaag ataagtaact ggagcaaata aaaacactta
    11281 ctgacaaaag ctacatagaa gatagattac ttatttttaa caaaaaagaa gtctaaaatc
    11341 ttctattttc taaaaaaaat cgaatttaat caagctgtaa agaaaaaaag ctttacaggg
    11401 taaaaaaaaa ctgttacact acatcttgtg aataaaacaa tggaggtgga aataaataat
    11461 ggcagttaaa atccgtttaa aacgtatggg ttctaaaaag agtccgtttt accgtatcgt
    11521 cgtagctgat tcacgttctc ctcgtgatgg acgtttcatc gaaactgtag gtacttacaa
    11581 tcctttgaaa gaccctgcag aagtagtttt aaaagaagac ttagttcttg actggttatc
    11641 aaaaggtgca caaccttctg acactgtacg taacatcctt tcaaaagaag gcgttatgca
    11701 aaaacaccac gaagctaaat tctcaaagaa ataaggtgac cggatatgaa agatttaagc
    11761 gacttagtct taacaatcgt tcgtccgcta gtcacttatc ctgatcaagt caagttagac
    11821 gtcgtagagt cgaaagactt ttacgagtac aatctaacag tggctcctga agatattggc
    11881 cgtatcattg gaaaacaagg gcgtgttgct aaagcaatcc gcacaatcgt ttacggtgtt
    11941 cggatcaatg caccgaaaaa agtgcgtttg aatatcatcg atgataagga aaagtaaata
    12001 aacgtgctgt actcgaattt tcgagtgcag cttttttgtt ttttttatta attgaagaaa
    12061 tgattatttg gcaaaacaag caagaaagcg tatccttata ggaggaggga gtgaacagcc
    12121 aatgagaaag aaaatgttat ttattccatt tgctctattg ttaagcgctt gtagttcggt
    12181 tactcaagaa tcagacaata cacatctatc aagcagtata tctgaaactg ttaccagtcc
    12241 agaaaaaagt agtaccaaaa cggcttccac cactgaacaa acaatggcta cctcaaataa
    12301 tgagaaaaag actgctttag accagttgaa agaacaacag ccaaatgttc ccatgcctct
    12361 agatgtacca gtctcttcag gctatctcaa tatcgcagcg acacatacaa agcaaggtta
    12421 ttctatttta tattacagaa cagatcgtcc cctcgggttg aatgctgacg aattgaatca
    12481 agaaacgcct attgcaacct atctatatca atatgggttt gcttctagtc aagaaacaat
    12541 ccaagttttg cagccatttg agatcgacac aaatggtcag caagtggact taggttctcg
    12601 aatcacaggg tatcagcaag gtgcagcagg ttcaagtttt cttgaatggc aagaaggaaa
    12661 ctggcgtatt cgaatccgtg ggaataatat agaaggccaa gatcccttac tattagctaa
    12721 agaaatcgta gcttatttgg aagaaaacag tctaccagca cctgaacaat ttggtaaaat
    12781 tactgttgat atgggtgata cgacgaatcg agccgtagaa gtaagctggc aagaaccgaa
    12841 aaatgcctat acgatcactc atcaagatcc tatgtctgcg ttgaagatgg cagtttctat
    12901 gaagaggttg tgaaatcagc gcgctgatct gagcccatca tttaactcca agtcacagga
    12961 agtaatcaaa aatagcacgc cgcatttttg cataattttg gcttaatacc ttaaaagaaa
    13021 actagaattg cctcttcaca catgctaaaa taaaacagac aaaaaagaaa tggggatata
    13081 agttgactga atacttgaat gtaggaaaaa tcgtaaacac acaaggtatc aaaggcgaag
    13141 tccgtgttat ctcaacaacg gatttcccag aagaacgtta caaaaaagga acggtgctaa
    13201 cactattcca agacggaaaa gcacctgtgg aattaacagt caaaagtcat cgcaaacata
    13261 aaaattttga cttattgagt tttgaaggac atccttctat caatgatgtg gaaaaatatc
    13321 gggatggcat attaaaagtg tcaaaagaca actctgtaga tctagctgaa aatgaattct
    13381 attaccatga gatcatcggt ctgcatgtat tagaagatgg aactgagtta ggtaaagtaa
    13441 aagaaatctt gtcaccaggt gccaatgatg tttgggtcgt tcaacgtccg aaaaaagctg
    13501 atatattgct tccatatatc gcttctgtcg tcaaagaagt cgatttagaa gctggagtgg
    13561 tccgtgtaga aattccagaa ggactgattg acgatgaaga ttgatgtatt gactttattt
    13621 ccaagaatgt ttgaaggtcc tatgggggaa tccatcatcg gtaaggctgt agataaagga
    13681 ttattagata tcaatatctc taacttccgc gattattcag ataacaaaca tcagacagta
    13741 gatgattatc catacggcgg tggagcaggt atgctgctca aagtccagcc catctatgat
    13801 aacatccaag ccattgaaaa agctgcaccg gatgtcaaaa aaagagtaat tcttcttgac
    13861 ccagctggta aacgattcga tcagaaaatg gcagaagaat tttcacaaga agaacactta
    13921 gtattcattt gtggtcatta tgagggatat gatgaacgta tccgcacatt agtgacagat
    13981 gaagtttcct taggagatta tgtccttact gggggagaat taggtgcgat ggtcatgatc
    14041 gatgcaacag ttcggctttt gccagatgtg ttagggaatc agacttcagc tcaaacggat
    14101 tcttattcaa ccggtttatt ggaacatccc caatatactc gcccggcaga atataaaggg
    14161 atgaaagtac cagaagtatt gacaaatgga aatcataaat tgattgaaga atggcaatta
    14221 aaagaatcac taagaaggac ctatcaacgt cggcctgatc ttttggaaac acttgaatgg
    14281 acacctcaaa tgacgaaatt tttagaagaa atcaagaaag aagaacaaga aaaaacagca
    14341 gatgaagcca agtgactttt tctaaaatct tctttacagt aggttgataa tatgttagta
    14401 ttttatggtg agtgcaaaag cacttaacta ttacgatatt ccgctgtgag aaaagctcat
    14461 aagaatattt ggaataagga gaagaaaacc atgaatccat taatcgaaga attaacaaaa
    14521 gaacaattac gttctgacat cccagcgttc cgccctggtg acactgtgcg tgttcacgca
    14581 aaagttgttg aaggtactcg tgaacgtatc cagttatttg aaggtgttgt tatcaaacgc
    14641 cgcggtgctg gaatcagcga aacttataca gtacgtaaag tttctaacgg agtgggcgtt
    14701 gaacgtacat tcccattgca tacaccacgt gttgcaaaaa tcgaagtagt tcgctacggt
    14761 aaagtacgtc gtgcaaaatt gtactaccta cgtgcattac acggaaaagc tgctcgtatc
    14821 aaagaaatcc gtcgttaatc atcgttatca gacagagaaa gaacccttgg tattcaaggg
    14881 ttcttttttt attatctgtt tttttatatt aacgatttct atttggtaca tttttgatta
    14941 ataaaaaggg gcaaaaaagg ggcaacaagt tacagttttt ctatatcttc tttcaatttt
    15001 tcttttactc cttttgtcac atgcaaataa attttttcag tgatatcact attttcgtga
    15061 ccaacgcgat cctgaatggc atacagagga gttcctagtt ccgctaattt tgaaatatgg
    15121 gtatgtctaa aaatatgtga actaagtttt ttatcaatct tcatgtcagc tttatgactt
    15181 ctcaaatagg tgttaattgc tgttagttgg aaaggagttc ctttcgttgt ttgaaataga
    15241 aattgaccat ttggatttaa ttctagaagc tcattgtaaa tagctatcgc tttttttggt
    15301 aaatcaattt ctcgcattcc ggcagctgtt tttgtagaat cagatttttt catatcagct
    15361 attgaacgtt ctctatacat catcgtccca tttataacta ctgatgcatt attattggta
    15421 atgtgtacat catctttact taatgcgatt gcttcaccag acctcatccc agtcaaatat
    15481 agccactgga aaaggagaga atatctttta ttgtgtgaag
//
LOCUS       DAJSKS010000059        15470 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJSKS010000059 DAJSKS010000000
VERSION     DAJSKS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.060"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            11..658
                     /locus_tag="ON396_001556"
     CDS             11..658
                     /locus_tag="ON396_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCR3113645.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            742..1686
                     /locus_tag="ON396_001557"
     CDS             742..1686
                     /locus_tag="ON396_001557"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HCR3113646.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1821..3272
                     /locus_tag="ON396_001558"
     CDS             1821..3272
                     /locus_tag="ON396_001558"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HCR3113647.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3293..4129
                     /locus_tag="ON396_001559"
     CDS             3293..4129
                     /locus_tag="ON396_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR3113648.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4129..4788
                     /locus_tag="ON396_001560"
     CDS             4129..4788
                     /locus_tag="ON396_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3113649.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            4914..5603
                     /locus_tag="ON396_001561"
     CDS             4914..5603
                     /locus_tag="ON396_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113650.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5609..6565
                     /locus_tag="ON396_001562"
     CDS             5609..6565
                     /locus_tag="ON396_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCR3113651.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6609..7121
                     /locus_tag="ON396_001563"
     CDS             6609..7121
                     /locus_tag="ON396_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3113652.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7377..7925)
                     /locus_tag="ON396_001564"
     CDS             complement(7377..7925)
                     /locus_tag="ON396_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3113653.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8014..9195)
                     /locus_tag="ON396_001565"
     CDS             complement(8014..9195)
                     /locus_tag="ON396_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR3113654.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9431..10939)
                     /locus_tag="ON396_001566"
     CDS             complement(9431..10939)
                     /locus_tag="ON396_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HCR3113655.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11098..11289
                     /locus_tag="ON396_001567"
     CDS             11098..11289
                     /locus_tag="ON396_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113656.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11492..12409
                     /locus_tag="ON396_001568"
     CDS             11492..12409
                     /locus_tag="ON396_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HCR3113657.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12402..13475
                     /locus_tag="ON396_001569"
     CDS             12402..13475
                     /locus_tag="ON396_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113658.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13623..14141
                     /locus_tag="ON396_001570"
     CDS             13623..14141
                     /locus_tag="ON396_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113659.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14122..14514
                     /locus_tag="ON396_001571"
     CDS             14122..14514
                     /locus_tag="ON396_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HCR3113660.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14590..>15470
                     /locus_tag="ON396_001572"
     CDS             14590..>15470
                     /locus_tag="ON396_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HCR3113661.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDK"
BASE COUNT         5111 a         2793 c         3113 g         4453 t
ORIGIN      
        1 gatcgaatca atgaaaaaaa ctgttattta tactagttta tttgcagctt tgtttttagc
       61 tgcttgtcaa tcaaatacca atcaaggcac atctgggaat acgtcttcca gtagcgaaca
      121 gcgctcttca caggtatcgg actcctcaac aaaagcaagc acatcttcgt ctagccagac
      181 aaaaagttct agtgaagcaa gtactcagtc agatatcaaa gtgtctgcag ctcgtgcgat
      241 cgaattgttc caagataaat acccagatac tgcaattact agtttggaat tagatagtga
      301 ttggggatct tacttctaca agatagaagg tgtcgatgat caaaaagaat acgaagcaag
      361 aattgatgct caaaatgaaa aaatggagac agagaatcca gaaactctag atcgagatga
      421 acaaaatggt caaaagaaaa aagaagatgg tctggatgtt gataaattga tcacgattga
      481 ggaagcagca aaaatcgccg tcgacaaagt aggaagcggt acggcaacag attgggattt
      541 ggataaagat ttaggtacta cttattggga agtcaaagta aaagacggca atcaaagtac
      601 atcagtcaag atcaatagcc aaacaggcga agtactgtca actgaacaag atgattaaga
      661 atttcttcgg aaaacaagag ttgatttttc ttttagtcat gatacactag acaaaataac
      721 ttgtttgaag gagcgattta cttgagttta gaaacgacct ttgccaatca tatctttgcc
      781 aatcctttga tgaatgcatc tggtgtccac tgcatgacta cccaggaatt agatgaactg
      841 gctcattcag aagcaggagc atttatcact aagagctgta cgatcaatga acgaaaagga
      901 aatcccgaac cacgctattt tgatgttcct ttagggagta tcaattctat gggacttcca
      961 aacttaggtt tttcttatta tctagagtat gctcttgcgt atgaaaaagt gcaggaaaac
     1021 caaaatcaac cgttattttt ttccattgcc gggatgagtg tccaggaaaa cttggaaatg
     1081 cttgagaaaa tagaaaaaag tgggtttaat gggatcactg aattaaattt atcttgtccc
     1141 aatgtgcctg gaaaaccaca actagcttac gattttgagg ccacttatga aacactgaaa
     1201 gaagtattca gcatcttttc taagccgcta ggaatcaaat tgcctcctta ttttgatttt
     1261 gctcattttg atcagatggc tgatatcttg aatcagtttc cattgaccta tgtcaatgct
     1321 atcaacagtg tgggcaatgg tttgtatatc gatacagaac aagaagcagt agtcatcaaa
     1381 ccaaaagaag ggttcggcgg cattggcgga gagtatatca agccgactgc tttggcgaat
     1441 gttcgcgctt tttatacacg tttgaaacca gaaatccaaa tcattggtac aggtgggatt
     1501 cggacaggac aagatgcatt tgaacatctg ctatgtggcg caagcatgtt acagattggt
     1561 accgagcttc ataaagaagg gccggaaatt ttttctagaa tcataaaaga attaacgcag
     1621 attatgtcag aaaaaggata tacgtcaatt gacgaattta aaggcaaatt acgcaccatt
     1681 tcatagcaaa gagacgaagg tcatgattga tcttcgtctt tttttataaa aaacactcgt
     1741 ctgttcattc ctgtgcaagc gtactcattg tatgttagac gaacttgtgg gaaaataaca
     1801 gaggcaaaaa ggaggataat atgaatgatg caacagaatt agcaaaagag cttaaagaaa
     1861 aagaatttag ttttcaagaa ctagttacgg atatacagaa aaaaataaag aagcaaaatc
     1921 atgaattgaa tgcgtttgtc gcgcttgaac cattcgagag taattcccac gagctattga
     1981 ttccccagtc agaaaaaggg ccattggctg gtatcccatt tcctttaaaa atgctcggtc
     2041 aagaaaaaaa gggatggcta gctacttcag gatctcgttt atttgaaaca caccgagctt
     2101 ctcatacatc taattatgtg cggcaagcag aagcgatagg tctagttcct tttggacaaa
     2161 ccaatgcgcc agagtttggt ttcaaaaata ttacagaccc agtcatctat ggcccagcaa
     2221 gaaacccttg gaaccttgaa tgtacaccgg gcggatcaag tggaggagct gctgctgcag
     2281 tttcatcagg aatcgttcct ttagctggtg ctagtgatgg cggtggttct attagaatac
     2341 ccgcttcgtt ttgcggattg attgggctga aaccttcgcg agggacgatg ccagttggtc
     2401 catatgcttg gagaggatgg caaggagctg ctattgattt cggcttgaca gtttctatga
     2461 gagacacgga ggctttattt tatgggatga gaagtatcca ttccggtgcg ccatatcaag
     2521 tatcgccagt tgagtggcaa acacatccaa gaaaaaaacg gttgaagata gctttttgtg
     2581 ttgattcacc gatccactca cagattagtg aagaggcagt acttgctgta aaaaaagctg
     2641 tagatttcct tggaaaagaa gggcatgatt tgacagagat tccttaccca ctagatggtc
     2701 gtaaactgat cacgtcttat tatcagatga atggcgcaga aacagcagca atgatccgct
     2761 cgattgaaac aggcatccag cgagctgtca gaaaagaaga acttgaaccc atttcttgga
     2821 ctttgttcca atatggtcag aagctctctg ccgctactta catcgattct ctgcatgtat
     2881 gggatgaagc agcaattgtc atggaaaacc tacatcaaga atacgatcta ttcctttcac
     2941 caacgactgc atttaccgcc cctaaaatca gcgaagattt gcaaagtgat gcgataagag
     3001 cacagatgga acacatcagt gattgttctg aagtagaagg tttagacgtt gtttcttcca
     3061 tgtttgagga gagcttgaag atcacacctt atacacagtt ggctaatctg actggacaac
     3121 cagcaatcag cttgcctact catttgacaa aagaaaacat gcctctagga atccaattta
     3181 tggctgccag aggaagagaa gatttgctat ttcaaatcgg ctatctattt gaagcaaatc
     3241 atcagttcca tttaccaaat ccatggaaat agcagaaaca aggagaagaa gaatgaagaa
     3301 aaaaagattg tttgctctaa taactagtgc cttttttatt ttaagcgctt gcggtacagc
     3361 tgccgctaat caggaagata acagctggag taatgtagaa aaaaagaaag aattagtagt
     3421 agcgacttct ggcacactgt ttcccgcatc ttattataat gatcaaaatg aattagttgg
     3481 ttatgatgta gatttggcaa aagaggtggc taaacggtta aatgtcaaaa tctcctttaa
     3541 agaatacaac gtggatggac aaatcacttc tgtttcaaaa agagaatcag attttgctgc
     3601 gaacgatttt ggtatcacaa aagaaagaga aaaaaaattc tccctttcag agactttcaa
     3661 atattcattt gacagtatga tcgtacgaaa aaaagatgat tcaggaatcc actcacttga
     3721 agatcttaaa gggaaaaaag cagctggcga acccaatact agctatatgc gtttagcgga
     3781 aaaacacgga gctaaattag tcacatatga caatgccacg aatgatcagt atttaagcga
     3841 tgtagcaaat ggtcgaacag atgttattct aaatgattat tatctgcaga aaatggccgt
     3901 atctgcttta ccagatatcc ctgtaaaaat actggagaac gtttatttca atgaaaacga
     3961 aagtggtttt ttattgaaag aaggaaatga tgctctgaaa aagaaaatcg atggcgtatt
     4021 aaaagagatg aaagaagatg ggacgacaaa gaaaatatct gaaaaatatt ttcaaactga
     4081 tgtttcagtc aaaccgaaag aaacgatggc tgagtcggtg agtgagtgat gtactttcct
     4141 ctaattgatt tttccctcat gtggacttca cttcctttcg tactgcaagg cttggtatac
     4201 acattaggga ttggcttcgt gtcgtttctt ttaggaaatc tggtagggtt gcttttgaca
     4261 gttttaggtc ttttggattg gctgcctctg aatgtattta ttcgttttta tttatcgttc
     4321 tttagaggca ttcccgcact agtcctgctg tttctgttat attttgggtt accttatcaa
     4381 ttaagtgcct taacggcttc agtcatctgc tttacgatta ccagcagtgc atttattggt
     4441 gaaatctatc gaggatcact agcaggcgtt tcttcaggtc agtgggacgc tggtttagct
     4501 tctggttttt ctttctttgg ggtattgaaa tatatcgttt tgccgcaagc ttttcgaatc
     4561 tcagtaccgg cactcagtaa cgtagctatg gatctagtga aaggtacttc gttagcagca
     4621 acaatcacag tgccagaaat atttcaaaaa gcaaagatca taggcggtcg gacattcgat
     4681 tatttttctc tctatatttt agttgcactg atttattggg tcatttgtat cgggatcgaa
     4741 aaagtgcaac agtatttaga aaaacgttct atgcatcagt caatctgaaa aatagaaatg
     4801 gtgctagcgt ggctggcacc atttctattt tttgtttctt gtatattctg cctagtattt
     4861 taaaaccatt tcatgtaaaa tgtaaatcga aaatatgttc ggaggtagaa aacatgaaga
     4921 ccccgctaac catcgaaata gaatcctcgt tataccaata ttgcatcgag caaggcggct
     4981 tggttgtaga agaagtcacg atgcctgatg accaaggaat tgtagatacg cttagctgct
     5041 tgacaaaatt tgatggaagc cgagaatggc gatgctatga gctgaaagtg acaaaagcag
     5101 attttcgctc tacagccaaa ctgtcatttg ttggacatta taattatttt gttttaccaa
     5161 gtaaactata tgaagaagta aaaactgaaa tccctacagg aataggtgtt ttagtgtatc
     5221 gtccgtacac ggttgaagag gagatgcctg cctctggtac atttctgatt gcgaaaaaag
     5281 cagcaaagaa agagcttgct gtaccggaag aagctttgac aaaccgcttc atggcttctt
     5341 tattcaggga agtaagaaaa gcaaagcaaa tggagcgagg cgtaaaatat tttccaacag
     5401 atcaattgta caaagaattg aaaagacgtt cagaaaatca agatccattt tctaacgaac
     5461 gatattatga acggttttta gaggaaatag ctagtcaggc aatcaatgag ttgcaagaag
     5521 aagttatcgc gcttcagcat gattatgaat atttgaggca acaacagcag atccgtcggt
     5581 tgcctacaga gccattggag tgaaaaatat gtactatcct tatttaagag gaaaacaatt
     5641 tgacctatta gcattaaaag aagcactgtc gcgaggattg ctctccaata aaatccaacc
     5701 agtgatcgaa ccagtacgtg attccgctac gcttaaaaat gtgatcgaat tatttcaaaa
     5761 aaagcaccat cgaatcgctg tgatccaaaa tccccaagta ggacaattca aattattcga
     5821 tcagcatgtg cacagttgga cagtcaaaga aaatagctct gtcgtccctg cacagatctt
     5881 aacgccagaa aatctcagtg aagtactgga ttcacctcca gcgtttcttg ttttcgatgg
     5941 acagcactac ccaaaagata cagaggtgtg gaaacagttg gcaggagttg attcgaaatt
     6001 tttgatacca gatactagtc gctttcgcat ctggctgccc gaaaacaaaa ttgtgatccg
     6061 tgactctttt caaacgagaa agcatgttga aagttatgca gataaaacag atgacttttt
     6121 tagtgatgac tatctttttt tccacgcaga cggatatatt ggattttctg atttcacgat
     6181 cgaaggtagc cgttatttcg ataaagggtt ccctagccga gcgcttgcta tccatctaac
     6241 gtatattgat gcttacggca atatacgcgt gaaacatttt gtctctgaca gtaacgactc
     6301 tgctaaagac caagcattga agtttcttga agcaggagat aaaatgaaaa aatggatcat
     6361 gcgtcaccat catcaactct tgattacttc aggaatgatt gaattattgg ctctttacca
     6421 gcaacaaaaa ttccctggac tgggagtgtt gaaaaagtgg tcacttatgc atcatttgga
     6481 acttattagt cacctgctag atcatccaaa agattggctt aaatattatt caaaagttca
     6541 ggaactatat gaagtgatcc aatgattaga taatgtaaat gaaaaaggaa tagaaaagag
     6601 gaaactggat gtatattcgg caaacaagga aagaagaagt agaagaagtg gtagaaatca
     6661 ttaatgccgc agcggatttc attgcttcgc aaggctctcc tcaatggcaa aacgggcaga
     6721 aaccaacaaa agaaacgatt ttgaacgata ttgaaagaaa agaaagctat gtattaatag
     6781 tggaagatca aattgctgga acagctgcat tagtctcaag cgtggatccc gtttacacaa
     6841 aaatagatgg tagttggaaa gaatcaactg gtccttattt atcgattcat cgtgtcgctt
     6901 tagcagaaag tactcgagga aaaaaactag caaaaccgtt cttagaacat ttgttgacga
     6961 ttgcagccag caaaaatatc caagatatcc gtatcgatac ttatccagct aatgaaccga
     7021 tggaaaagac catctattct gctggattca cttatcaagg gatgattgaa tttccttttg
     7081 aacacggcga aagaaaagca tatcaaataa tcatcaattg actataaaat catctgagca
     7141 aagataaaga cattaacact actgaaaaaa acaatcatat gatagccaag aggttgtaac
     7201 agaagtggcc agcttcaaaa aacaagaaag aatttccgaa aatgcttctc aaatttttgt
     7261 ggaattttag cgtattttac gaaatagctg ctttctgaaa ctcccttcat cgagaggtcg
     7321 tgacatagct tttgtcacga cctctcggct tatcctacga gtattattcg atacatttat
     7381 atcaataatc ctttttttat tttatctgca gcttcttgga acgctccatc gtttgacacg
     7441 actaaaagtg aggggatagc tttcaaacgt ttcaactgtg ctaaatcttc tacataaacc
     7501 gccagtctgg ctgaaatctg ttcagatgaa tctccacgtt ttttcaggcg cgattgcaat
     7561 gtttctttag agatcgtaat aaagatagga cggactaccg tcccgaaata ctcatataaa
     7621 tgccaaaaac catttgctgt caaaacggcg taacaatttc ctactgtcag tttcttttta
     7681 atttctgctt tactggaacc atatttatgt tgagcatatt ggtcccactc tgcaaactca
     7741 tttcccttga tcatctgatt gaatgtattg tcagaaacaa aataataatc ctcttgatct
     7801 ttttccccag gtcttggtga tcgattcgtg taagtaatga tcttttgttc cggctgaaaa
     7861 atagcttctg cgagtgaagt ttttccagaa ccagaaggac cgataaatac atagcaataa
     7921 ttcattgtca tttctccttt gatgattgaa caagtgaaaa agctttggaa aatatttcca
     7981 aagcttttaa aagtaacttt gcttttttgt atattatacc agcaagccag tcacaatagc
     8041 acttaaaaag ctgactaaag tcgctgttag caaaattttc aatccgtgtt ttgcgacaag
     8101 atttcctttt tcttcattta atcccttcat cgcaccagaa atgatcccga tagaagagaa
     8161 gttggcaaat gaaacgagga agacagatat aattgctgtt gtacgctcag taaaatggaa
     8221 attaccattt gctaattctg tcatagcaac aaattcattt gtaaccagtt tagtcgccat
     8281 gatactaccg gcatccactg cttctttcca tgggattccg ctgatgaagg caagaggggc
     8341 aaagatgtaa cctaggatct cttggaaagt gatgccgaat atcccgtgga agatagcatt
     8401 gatcatggca atcaaagcaa caaaaccgat aagcatagca gcaaccgtga tcgccacatg
     8461 gaaaccatca agaatgtatt ctcccaacat ttggaaaaat gtctgttctt tgttttcttc
     8521 aatgatcagc tcatcttctt tttcttctaa tacataggga tttacaattg aagccagaat
     8581 ataaccgcca aataagttca aaacaagggc agtgatcaca tatttagaat cgatcagtgc
     8641 catataagat ccgacgatcg acatagaaac tgtagacata gccgatacac tcatggtgaa
     8701 caaacgtttc tcagatagga agggtaattc cttttttatg gaaataaaaa cttccgattg
     8761 tcctaaaata gcagaagcta ctccattata agattccaat ttgcccatcc cgttgatttt
     8821 acttaatcct agaccaacag ctttcataaa taatggaaga atcttgatat agcgtagaat
     8881 accgataata gcagaaataa atacaattgg catcaatacg gaaagaaaga aaggtgcact
     8941 tcctttattt gctacaccgg caaaaacaaa gtcaacacct tgcccagcaa aagcaagaag
     9001 atgatcaaac atttttgcca atacagaaac aaccgctgta ccaaaagttg tacgtaataa
     9061 tacgaaacca agaacaagct gtaaaacgaa catcacaatt agtggtttca atttgattgc
     9121 ttttttgtta ctgctgatca aaaaagcaag ccctaatgtg acaaataaac caataatacc
     9181 taataaataa ctcaaataat cactcctatt ccaaattccg ttttatttta cctgttttgc
     9241 aaataaaata ctaatcttta gtgaaatttt cattactttt ttagagtgaa acgcattcat
     9301 tttccattag aaaataaagc gaacaaaata aaaggtttaa aaagtgtaac gtcttagtaa
     9361 agtctataaa aaaacgaaac tatcgattaa gctaatagtt tcgttttcaa catttataag
     9421 taaacttttt ctattggtaa tatagatttt cggaagggta gacaggtgcg caggtaatat
     9481 cgattcctag ttttttcaat gtttgttcat catttttatt gaccatcaca gtagagtggg
     9541 cttgtgctcc ctcaagttca gctagtttat cataagcaac ttgagcgatt ggattcgtta
     9601 ctgcactgat tgataaagcg atcaagactt cattgatgct tagtgcatcc aaagaggaat
     9661 gaagtgtctt tgatttcaat ccttgtattg tttcaagaac taatggtgac aataaaggaa
     9721 tgtcatctgc gatatgggct aaatgtttta ctgcatttaa gataactgcc gctgacgcat
     9781 ccatcaatga acttgtacgc ccggtaatga tgacttcatc tggtaattcg attgcaatca
     9841 cagcagaagg atcttttgta ggactttgcg cttgaatctt atctgcatag tcatgggcag
     9901 ggactaccac gtttcggtct tcaggacgta aaccgacttc ttccataatc agtttgattc
     9961 gattaactgc ttcttcgtcg atcaatcctt ttttgaagtc gcattctgtt tggaaataac
    10021 gacggatgat ttcttgttta gaggcttctt ggacaacggt atcatccacg atcccaaatc
    10081 ctacacgatt cacacccatg tcagtaggag attgataaac agattcttta ccagtaattt
    10141 tttcaataat acgtttgatt acaggaaatg tctcaacatc ccgattatag ttcacagcta
    10201 ctttattgta ggcatcaaag tggaaggaat cgatcatatt gacatctttt agatcgactg
    10261 tggctgcttc ataagcaatg ttcagcggat gtttcaacgg aacattccat actgggaaag
    10321 tttcgaactt ggagtaacca gctgcatgtc ctttttggct ttcatgatac agttggttca
    10381 aggaagtagc gagctttcca cttcctgggc caggagctgt cactacaacg attggtttag
    10441 ttgtttcaat atactcattt tttccaaaac catcttctga gacgattttt tctacgttga
    10501 ctggatagtc ttcgatttct gagtgtttat agaccttgat gtttcgtctt tctagtttat
    10561 tgataaagac tttcgtggca ggctgtccac tatagcgagt gataacgaca ctgttgatta
    10621 ataagccata accacgtaat tcatcgatta accgcaagat atccatatca taagtaatcc
    10681 cataatcacc acggatctta ttacgttcaa tatctccagc atagacgcaa atcagtattt
    10741 cagcttgatc tttcaatttt tgcagcaatt tgattttcgc atcttcttca aatcctggaa
    10801 gtacacgttt cgcatgcttg tcgtcaacta gcttcccacc aaattctaaa tataatttgt
    10861 catagtggtt cactcgttcc aaaatatacg ctgactgctc ttcaatatat ttttctgaat
    10921 caaaaccaat ttttttcact aggttccctc cttaagtact aaagcatttt ttcatttttt
    10981 tgaggattcg tcaactggaa gagcgaaatt cctcaaaaaa acaaagaaaa tggtttaaac
    11041 caacttgtct agatagttat tcattggtat gatagttttt gataaggaag gtgctacatg
    11101 aaaaaaagac agaaaaagaa aaacgcatat aaacactata tccgttcgat ttttaccggt
    11161 tatgaaagaa tgcttgaaga tcccgaattg gaacaattga cgtttactta tctaaacgaa
    11221 gaaacgcagc taactcgtga tgagcatcag cgcattcatt tcacaacaag agatctgcct
    11281 agtaaataaa aaataagata aaggtcatcg gattcgattc tgtcctatca gatggaaaga
    11341 ctagttgatc gaaaaaaagg ctgtaccaaa ttttggtgca gcctgtttta tggagcattg
    11401 cccgctcttt tttataacaa ttttttacag aaaatggtac tattgaagta ttaataagtg
    11461 aaaaacagat gagtaagagg aggcaagtag tatgaaagag ccagatacaa atgcaaatta
    11521 tgagccagac gaactagagg agacgaatgc ggatcaggat acaggacaag aatcgtcttc
    11581 tttttacgat atcaaagaaa aagcaagcat gaatctttcc caattattag aagacttaca
    11641 ggcttttgaa aaagctgtcc gcgaagaaga tatccctgag atctaccgtt tatacaatgg
    11701 ccgattgaat gaagaattaa aaagaagctc aaatgaaaat cacgagatcg atcaattgct
    11761 gatgcgtaag atccatgatc gttttttaca ggaatttcct tttatggaac aagtagaaaa
    11821 catctcgcca acgatgagct attacaaaat cggcacttat taccatgatc ggcccacggt
    11881 cggtattgat gcaagtctgc cagaaatttt tgttttgcct aagatcgatg aagagtggga
    11941 gaaatattcc caaccggagt caacggacta tgacaaaaaa atcaatgagc tagacgcaaa
    12001 ggtgatcact gctcagacag aaattgaacg tttagatgaa caaataaaag aaatcaatcg
    12061 acaaatgact gatttgaacg acaataaagg attcttgaat cgtaaaaaaa tcgaggaaga
    12121 aattcaagaa ttagagaaga agaagcaagt actatctaat gaaaaattag gctggcttcc
    12181 ttatattgag acgcctgaaa caatccagca gcaaaaagaa gcattgaagc aagaagcaag
    12241 agcagaccag cttcgtgcag ccatcgtaga aaaagaacaa aggcaaatca gacgctattt
    12301 cggtagcaaa gagggattcg gtcaagcaat ccatgaattc ttgatgaatt atcttggcaa
    12361 tgagaatcct aataatcaat ctaatgaagg aggttccgag tatgaatgaa cacgatgaag
    12421 cagaatggac aaaaaatgaa gaacaaacag aagatcatga agaaacgaac acttcattgg
    12481 aagaacaaac aagtaatcct ctggatgaca cagaagaaat ccctatgcta gaggaaacaa
    12541 aaatacacga aaaagaaaaa gtaactccga cgaatacgaa tactgcagaa aacacccatg
    12601 cttacgaaat aactgaaaat cccgggtatt tagaaggaaa agagcaagat catcaagata
    12661 tcgtcagttt gattgaaaca gcggaagcag aattagcgga tttacgtatg agaaaagatt
    12721 tgatcgaatc agattttcca caattattag aatcatatga tcgttttata ttgaatgaag
    12781 aaccaatctc cacgatcgga aagacaagcc tgatcgaata cttcacgtat gaattactga
    12841 aaccgttgaa tgatattggt ctggaacggt caaattcagc ttatgacaca tggaaaacaa
    12901 gacatttttc gatcaattat acgttgaatg gacaaaacga attagtcttt tcctttgtat
    12961 taccgaccat cgatcaacga taccaagtgg aatcgatgag tctcctaaac gtatcaccag
    13021 agacaatgga aatcaatgtc caagatgacc gggtgctttc tttgatccga cactggtcag
    13081 tggatcgagt attctctgca gggcagatca cgatattcaa tcacaaattg aatcaattat
    13141 tagcccatgc aagaacactt ggttttaccg ttaatcagac attactagac aatacgaagc
    13201 cacttcgttt gaatcttcag agcgaatttg aattgacgaa tgaagtattg gatgatattt
    13261 ttatcgtggc aatgaaaaat ccatcctacg atatggaaaa gatcaacgaa actacttatc
    13321 aagtacgatt agataaagga cagtctttaa cgataacaac aaacgaaaaa gatcaaacag
    13381 tcttgtcgat ctcatctgga gactacccac gatctgtcat tgatttcttt atcaattacg
    13441 aattcttggt cccattaatg gtgagaaagt cctgattgga taagttggat taccagatgt
    13501 cattcagata attctgttcc aaaggacttg cggcagttat ccagaataat tgtcacaagt
    13561 cttttctgta tgtactaaat ggacgtgaat aacttgctga tgaaggaggg atttcattag
    13621 gaatgtccac aaataaaata gcacagctta gcttattgtc tgctgcttgt gtagcaggta
    13681 ggatcgcttt tcaatttatt ccgaatttcc agccaatgac tgctattttt ttatttatag
    13741 tactttactt aagcttaaaa gatgcgttaa ttgtcatgag tctttctatt gcgatcagca
    13801 gtttttattt tggtgttgga ccatgggtca tcggccaatg gatcagctat actgttgttc
    13861 tgagtttgtt ttcaatcgtc tgcttgagaa aaacagtgca aaggaatcta tggttaaagg
    13921 gagtctgttt ttttctggcg ggcatggttt acggaatagg tatgaccgtt tttgatacgc
    13981 tgttatatcg tttgcctcaa ccttggatct attaccttca aggggtgtcg tttgatttga
    14041 tgcacagtat aggcaatgtt gtgttctttc ttgtttttct gccagtcgtc aaacgttttt
    14101 acaatcattc aggaggaaaa aatgaaaaaa ataatttata gtttgcttgc aatgagtgtc
    14161 agtatactca ttttgacaag ttgcagtaca acctatacac aatcggcttc caaccaaaag
    14221 gaggaagtaa aaacatcgca aaaagcaaca atcacgttac aagaaaatgg tcaaaacttt
    14281 tcggaaaaag aagtgttctt caaaaaaggc gatgatttac tgagtatttt gaaacgaaat
    14341 tttgaagtca aagaagataa cggcttcatc acttcgcttg aagggcactc acaggatgag
    14401 aaaaatcaac tctattggat gtttactgtg gacgggaaaa gtgctactac tggtgccaaa
    14461 gagatcaaat tgaatgatgg acaatcggtc attttcaatt tgtctaaact ataagtttca
    14521 taaaaaactg ttaaagggcg aatattgcca taattcagtg agttcagttg atgaaagaag
    14581 gaaaataaca tggaaaattt taaaaaaagc ggaatgtttt ccgtaattgc tgctctaggg
    14641 gctaatattc tagttgctat cagcaaattt gtcggctatg caatcagtgg cagtgccgcc
    14701 atgctaaacg aaagcatcca cagtatcgtt gattgcagca atcagatttt gctgttattc
    14761 ggtgataaac aagccaataa aggacagagc gagttgcatc aatttggtga aggacgagca
    14821 aaatactttt tcagtacaat cgtcgcaatg atgcttttct tcggcggtgg ggcacttggg
    14881 gtgatggaag cttttgaaaa gttgacgcat ccatcccatg aagtgggtaa tccgatcatt
    14941 gttatagcaa tactgttatt tggtttggta atcgaaggaa gttcgttacg cgtggcaatg
    15001 aaagaaatca aagaattgaa tactgaaaaa ctttccacga tgcgcttttt gcgcgaaagt
    15061 cgacacagtg aaatactgat tatttttacg gaagacttgt gtgctgtaat cggtttggtc
    15121 ttggctttag caggaacaat attgacttat gcaaccggga ttgctgcatt tgatgcgatc
    15181 agcggattgc tgatcggttt cttgctgatg ggagccgcca ttttcctagc aaaagaattt
    15241 tatagtctga tcatcggtga aagcgtgaca gcaagcgatt tatcaaaaat caagttagct
    15301 tttgaacgtc ctgagatcag tcggctgatc gatataaaaa cagttcattt aagtccaacg
    15361 gagattttag ttgcagcaaa agcagatatc gttggtaaca aagagaatga agcttactcg
    15421 gtcatcaatg atattgaaaa aaatatgcgt cagtctcttc ctgacaagaa
//
LOCUS       DAJSKS010000060        15402 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJSKS010000060 DAJSKS010000000
VERSION     DAJSKS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.061"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            138..2198
                     /locus_tag="ON396_001573"
     CDS             138..2198
                     /locus_tag="ON396_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HCR3113662.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2281..2850
                     /locus_tag="ON396_001574"
     CDS             2281..2850
                     /locus_tag="ON396_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3113663.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3025..3831)
                     /locus_tag="ON396_001575"
     CDS             complement(3025..3831)
                     /locus_tag="ON396_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113664.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3969..5483
                     /locus_tag="ON396_001576"
     CDS             3969..5483
                     /locus_tag="ON396_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HCR3113665.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5886..6869)
                     /locus_tag="ON396_001577"
     CDS             complement(5886..6869)
                     /locus_tag="ON396_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HCR3113666.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6874..7521)
                     /locus_tag="ON396_001578"
     CDS             complement(6874..7521)
                     /locus_tag="ON396_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3113667.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7606..8238)
                     /locus_tag="ON396_001579"
     CDS             complement(7606..8238)
                     /locus_tag="ON396_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3113668.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8254..8805)
                     /gene="coaC"
                     /locus_tag="ON396_001580"
     CDS             complement(8254..8805)
                     /gene="coaC"
                     /locus_tag="ON396_001580"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HCR3113669.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8802..9539)
                     /locus_tag="ON396_001581"
     CDS             complement(8802..9539)
                     /locus_tag="ON396_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HCR3113670.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9536..10525)
                     /locus_tag="ON396_001582"
     CDS             complement(9536..10525)
                     /locus_tag="ON396_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR3113671.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10783..11325
                     /locus_tag="ON396_001583"
     CDS             10783..11325
                     /locus_tag="ON396_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3113672.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11342..12427
                     /locus_tag="ON396_001584"
     CDS             11342..12427
                     /locus_tag="ON396_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3113673.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12439..13245
                     /locus_tag="ON396_001585"
     CDS             12439..13245
                     /locus_tag="ON396_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3113674.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13246..14073
                     /locus_tag="ON396_001586"
     CDS             13246..14073
                     /locus_tag="ON396_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3113675.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14070..15143
                     /locus_tag="ON396_001587"
     CDS             14070..15143
                     /locus_tag="ON396_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3113676.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         4953 a         2780 c         3137 g         4532 t
ORIGIN      
        1 ttttcttgaa acaataaaaa atcaaaaaaa atactctcct aatagtccat cttcttttga
       61 ttttatttat ataatgaaat catcttatga aaattactac ttcatgttta gacaagagta
      121 gggaggacta agaataaatg tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt
      181 gtcgtttaga taaggaacgg tcgtgttctg aaaaacacta cgttaagttt atgatgcgca
      241 cgatgctgat cagtttatct gtaggtgtct atatgcatgt acccgttcag gcaacgacac
      301 aaaccatcaa ggtcgtacgt acctctgttt ctgagaccac tgattcccct atcactatta
      361 ctcatacttt cactgatgca aatggaaaac agctacttcc tgcattgact tcctataata
      421 aagtcagtta tcctccaacg ataaaccact actggttaat tcctaacaaa acattatgga
      481 catcggaaac cggaagcagt gtgccgcttt ctatcttttc cgatatgact gatcctacaa
      541 aaggtgatca gttaaaacag atcactgata ttttgaatca tgctttgaca gatataaagg
      601 acggtggaag cgcacaagtt tcttatgtat atgaaaaaga ttattcacag tttgaaagaa
      661 agaaaatcga tgttctggca gggacaaaag tttcgatatc tgatctggta gaaaggctga
      721 agaacgcaga tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg
      781 tggatacgac taatacagga acatctacta tttccttgtc ctattttgat ccaattgctt
      841 taaaaacaat gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag
      901 gaaaagatct tcacttaaga acaggagaga gttacgcccc taaagatctt gttgaatatg
      961 taacaaaaag cgatggaact tcaggaaata aagaagaaat caaaatgaac gggacagtag
     1021 atactacaac tccgggagaa tatcccgttg tattaactta tatagactcg aaaacatcaa
     1081 atgtggcaga aacaacagct gtagtttatg tagaagcatc tgctgcttca tcgactgtca
     1141 tcactcattt tatagatagt caaacaaata aagaacttca agcagaaaca catatgagta
     1201 gtgtaaatcg tattcctgat ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata
     1261 tagatttatc gacttatgaa caaaatggga aagtcacttc tatttctgat tggatgcaag
     1321 caaacggatt agacagaaaa agtcactggt cggagatcct tgaagtactt cactcagaac
     1381 tgatgaataa taggaagcaa tcaggagggg aaattacctt tacttatgtt tatataccag
     1441 acaatcgttc attagaaggt catgatctta ttgttcatcc aaacgaaaaa ataaacaaag
     1501 aagagttgat ccaatcagca acaaattcct taggtaaacc cgtaccgtta aaggatgtat
     1561 cggttacaat caatgaagta ccattggagg atggttattt gaccaaacaa acaggtgatt
     1621 ttacgattat gtatagttat catgacccgt attcactcca agaactccat gctcagtcac
     1681 tattacatgt tttaccatta aacaaacaga tagatcaaaa agaacaaaaa agacaagaag
     1741 aaagcttgtc agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa
     1801 aacaaacaag aagcctagag ttagcaagaa tatctagaaa aatctcagaa tctaataaaa
     1861 cgataaggca agaagctcat ttggccccac aagtttctgg attggaaaat gaaattttag
     1921 agcctaaaat aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg gaagatcaga
     1981 aggaatcaga tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt gattccaccg
     2041 atgagcatct gcagccgcaa gtaccacttc ctagtccggg aacacccgca cctggtggag
     2101 gtattccttc ttctgcttta ggagatttca tgcgaggtat ggcaggaaca tgggtatacg
     2161 ctaatcgaaa tgaatttgac gcagatgact ggttctgatt tatatctcta tctgttatag
     2221 tcaaataagt agctgagtca tatgttattt ggcataatgg agacgaggag ggatagaaaa
     2281 atgttagata aaacgttacc ttacgcagaa atctggatga caagaccact tcaacaaaat
     2341 gttccaatca cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa
     2401 tctgcttggg cggagattga gacttccgtt ggggaatttc aaacgacaga tgaagcactt
     2461 gagtattttc aacaaaactt tgctccttat cctgaagaac ttgaaagaag gatgtttttt
     2521 gtagagacaa aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga
     2581 gagcaagtgt atccggtttt tcattggtta gcagtgaaac cagattacca aggaaaaggg
     2641 ctcgcaaaag cattgactgc cagagtcttg caagaatttc ctaccctcca tacggacgga
     2701 ccaatctatc ttcacacaca gacatggagc catcaggcga ttgctcttta taaaaaaatg
     2761 ggttttactt ttattgctga aaatctagac ggcacaccta atccagatta tgaaaaagtt
     2821 atgcaggttt taagtaaatt agataaataa cgaaatggtg aaataagtcc tcagtattgg
     2881 gataaaaaac gaaatagtca ttcctcatat cttacttttt tgagttaata ctatcggata
     2941 actaatggca tgccatacat tcaaaaaaga aggggcagtg acaaaagtat tgtcactgcc
     3001 ccttctcttg ttttttagca tgttttatcg tttgattttt ttaggaataa aattcttgat
     3061 gaacgtaaat ttttggtaat cgtttcgact atagaggtaa ccaaatacac tgaaaacaac
     3121 tgctccaata aaattaacaa ataaatcctt catagtatcc atgattccca cgtctaagta
     3181 accattttgg atgattgttt tatctgtttt accatctgca ttttgacttt caatgattgt
     3241 ccgagtgata tctggaacat gatagacagt gtttccgtct ttacttaact ccacactatt
     3301 gatttgctgg atgatcgtat ctttttgcat atcgaattgc agtaatttat ctgctgaaaa
     3361 ttccacaaac tcccacataa caccaactgt catcgaaaaa cagaaggaaa caactgctag
     3421 taataaagga gtcaagttca tggtttttgt atttttattg agcaaataaa caagggaaaa
     3481 gccgacaccc ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc acattgaaaa
     3541 atagccataa aaatcactta actctcctaa aactgttgaa ctaaaaataa aagataacaa
     3601 aatgatttct agcatatttg gaatctgtat ctttaatatc ttttccagaa aataagggat
     3661 ttcaaataaa attaatgtca gtaataaaag cattgtattt ccccagtgtt ggttccagac
     3721 ctgatccact agcataataa cgacgatgat acgtaataaa atatagattc cgtttgtgat
     3781 tctacctgca gtttttgtac tgttcgtaaa aaaggttttc aaatgattca taaaataact
     3841 ccttcatgta tatccttaat tatactaaga agcgtgtgtt taccaaagct atatgactta
     3901 aagaaaaaca gagttgatat tgtgaaaaaa atcacttaat atagtataag gagaaaaggg
     3961 ggatacaaat gaaaaaatta gttgctggtt tattaacagc cgttgcactt cttgctatga
     4021 gcgcatgcgg cgcagatcag aaaaatacag cgcagacaag ttctagtacg aaggaatctg
     4081 ccgaagtatc atcaggcgct tctgaacaag tatatacaga tccaaaagaa atgaaagaat
     4141 cttatgatgt cgtgattatt ggagctggtg gtgcagggat ggcagcagct atcgaagcaa
     4201 aagatgctgg gttgaatcca gtaattttgg aaaagatgcc tgttgccgga ggaaacacat
     4261 ctaaatcatc aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa gaaggaatca
     4321 aagactcgaa tgatgccttc tatgaagaaa cgctaaaagg tggtggagga acgaatgata
     4381 aagaaatgct tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg ctagatcaaa
     4441 acggtatcaa actggacaac ttaacgatca ctggagggat gagtgaaaag aggacacatc
     4501 gtccgtctga tggctcggct attggtggat acttagtgga ggggttattg aataacgtcc
     4561 atgagcggga aatccctatt tttgtcaacg cagatgtgac agacattaag aaaaatgggg
     4621 aaacggtcaa tgaagtgact gtacatgttc aaggagaaga accaaaagag gtcaccggaa
     4681 aagcaatcgt agttaccaca ggcggttttg gtgccagcaa agaatttatt gaaaaatatc
     4741 gtcccgattt agctgattat attacgacaa atcaagaagg atcaactgga gatggtatca
     4801 ccatgattga aaaattaggt ggacaaaccg ttgatatgga taaaatacaa attcatccaa
     4861 ctgtgcagca ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa ggagcaatct
     4921 tggtcgatca agaggggaaa agatttgtca atgaaatgga tacaagggat aaagtatctg
     4981 cagcaattac agcattgcct gaaaaatcag cttatttgat ttttgaccaa ggcgtgcgtg
     5041 atcgtgccac agctattgaa ttttatgacc aaaaaggata tgtcacaact ggaaagacaa
     5101 tggaagaatt agcagaaaaa atcgatttac caaaagaaac actagcaaaa acagttgatg
     5161 gatggaatac ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc acagccatgg
     5221 aacatgactt atcgtctccg aattattatg cgatcaaaat cgcaccagga atccattaca
     5281 cgatgggcgg tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa aaccagccaa
     5341 ttagggggtt atatgctgct ggtgaagtca caggtggctt acatggaaac aaccggatcg
     5401 gtggaaattc agttgcggag atcattattt ttgggcgaca agcgggcata caagcagctc
     5461 gatttgcttc ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt gaaatcagcg
     5521 ttttgagcta ataccgaggg tcagctgatt gaacactgtt tagtgaagag gttgtgaaaa
     5581 agctgccctg catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttaac
     5641 ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttattcg gaaaaagagc
     5701 gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag
     5761 cagaagaaat tccttcggaa ataagctgaa attcacaaaa atttgaaaag caattttcgt
     5821 gaatttcttc ttatttcttg gagttaaacg cttctgtccc agcctctttt ttgatcagca
     5881 tgattttatt cagttatctt caaaatgatt tttccgatgt ttttgtttgc ttccatatat
     5941 tcatgcgctt gtttgactgc gctgaattgg aatgtacggt caatgattgg ccgaatcttg
     6001 ccattttcta gaaaaggccc agctttttgc ataaaagttt gtgcgagctc tgctttatac
     6061 tgatcacttc taggtgtcaa taatgtacca agcagttgga cacgtttccc aatcaataca
     6121 ccaaggtcta tctcaggcac gatggtccca cctagcatac caatcaagac ccaacgtcca
     6181 tcaacggcta tagaagctaa attcttttcc cagtaagaag cgccgatgaa atccaaaata
     6241 acatcgactc cccgaccttc agtgattttt cgtacctctt cttgaaagtc ttgtttttta
     6301 tagttgatca attcatctgc accaagttct ttacaaaagg cgagtttttc ttctgatcct
     6361 gcagtaacga tgattttcgc gtgagaaagc tgtctagcta attggatcgc tgctgttccc
     6421 acaccacttg ctcctgcatg gatcaagaca gtttcgcctt cttgtaattt tccaagccag
     6481 taaagtgttt gataagcagt caaaaaaact tctgagattc cagcagcaga aacatagtct
     6541 agcgtatcag ggagaaggat tgcacgatcg gcaggcatca cagcatattc ggcataacca
     6601 cctagattga ctaatccgta tacttttgtc ccaggagtaa aagtggagat atcactttta
     6661 ttctcaacta cctctccagc tacttcgact cccaaaatag ggtagggagg tttcaaagag
     6721 actgattctc ttttcataat atctgttcga ttgacagctg ctgtatggac tttgatcagt
     6781 aattcgcctt gttttggttt aggaacaggc acttcaattt gttctaatac atctacagaa
     6841 ccaggatgct tgattgaaat ggcacgcatg gatctattcc tcctttgccc attcttggaa
     6901 tgctgtattc aactctttgc taccttcaga aagtactaga gtcgtacgag aaatatccgt
     6961 ttcttccact agagcggcaa gtacagcagc aactgtagag cgagtgatcg tttttgatgc
     7021 cttctcatta ggcttagtta attgaatgga agtaacttct tcatcgttag tcaatgtgac
     7081 tggttgaaga atgacaaaat ctaagttagt tcgtttcagc cattcatctg cataatgttt
     7141 agtaatataa taatctgtca tggactcagg ccattttgct ggttcatctg cataaacagc
     7201 actgaccatc agatagcgag agacatgcgc attttcagct gcgatcactg ttttgattgc
     7261 gccatccaag tctacttgca gaagattctt tccttgagag ccggcagcaa aaatcaatac
     7321 atcgatgcct ttgaatgctt cggtcatttt ttccacggac caagttaaat caaaagagac
     7381 atacgaaaca ttctcttttt ttactaatgt ttgggaagca acatctctca cccctgcaaa
     7441 aatttcatgg gaacttggag ctaaatcttt tatcaggtgt cggccaattt gtccgtttgc
     7501 acctacaaca aaaattttca tttggatcat cctttcgacg tcaaatcttt ccattcaata
     7561 gtaacaccaa aagagaaaaa aatcagagat cggctacgat gactatcagt tagtaagaga
     7621 agggtgcttg aagtatctta acaagacatt gcatactgct gcagtcgcaa gacctgcgac
     7681 gattccttca ggtataccac ttgtaaaaat aaccgctaga atagctgaat aaacagcatt
     7741 taaatttgtt ccgatgactt tggcataatc ttgctggaat aaaaagtaga tcatattcat
     7801 cactaaaatc gtattggtca atgaaccgga aagtccagct aagaataaag aaacggaatc
     7861 tgctttttgt ttcattagtt tgcgcagacc tttataaacg aaataaggaa ccaccccaat
     7921 caagatgcga ggaataaacg cgatcagcaa ggctttccag cttccatgtt ctgttccgat
     7981 gactgggata aacggagaaa agacaaaaga caaaggtgtt tggataactg tgctacggat
     8041 catgctgatc agtccaaatg taccgccaag aaaagcacct agtcttggtc ctaaaacgat
     8101 cgaagcaatg attacaggaa tatgcatggt tgttgcattg attggaccga tagggataaa
     8161 acctaaaggc gtaacagcta ataaaatcaa aatagctaaa aacatcgcag ttaatgtaaa
     8221 gttttttgta tttttcataa gtactccttt tttctaagca tttctttcat taagagtcag
     8281 catgacctgt tcgatgatcg tctgaatatc agctaaggca ccttttccaa aatctccaca
     8341 agctaaaaga gattctcttg gttcaatttc ctgatagccg acagatctca atgtttccag
     8401 attacgctga gtgatcgggt tttgatacat attcgtgttc atggcagggg caatcaattt
     8461 tggtgttccc actggaagcg ccattgcgac tgttgaaaga agatcgtctg caatgccgtt
     8521 tgctagcttg ccgataatgt ttgctgtagc aggcgcgatg agaaacaagt cagcttgttt
     8581 tgctagttcg atatgattga ttacctgcgg atttgattct tgcatgacgt ctgtatggac
     8641 tgcgcgcttc gacagagatt gcaaggttaa tggcgtgata aaatgtgtgc tgcttttaga
     8701 cataatcaca tctacctggg cacctaattt gacgaattga cttgtaagat cagcgctttt
     8761 gtaagcagaa atacttccag taacgcctag taagatcttt ttcattttga ttcctccagt
     8821 ctaattttct cagtaatcaa tgcagcaatc tcagatttac tttgagcttc ttccactgtt
     8881 ccatctttag ataagagata gccgtggtgt gtctcgccat ggatttccgt caaatcattt
     8941 gcgaaaataa aatcagtttt gttatttatc agactatttt ttgcgactgc cagaagagat
     9001 tcctttggta catctactaa tagtttgaaa ccaatgattt ttgcttttgg ctgccattca
     9061 cgtagataag aaatgatttt tggtgttttc ttcaaaacga gcacaagatg ctcggtgtta
     9121 gaagagatct ttgaaacagt ggtatctgtc tgttcatttt ccgcaaacca attgtccagt
     9181 tcttcttgcg ttgaagaagt gggtaggttt tttgccagct gttcttctga aaaactaaaa
     9241 gcaggagtaa aatcactgac tgccatactg tggataatcg catcgtagtg ttcttttttc
     9301 gttagttctt ctaattgtaa aaataagtcc agtgcagatt cgatcgtata aatcgttata
     9361 ttggagtgtc ttttcggttt cagtgcttgt ttcgtagtca cgtaatcgat tgtggtatcc
     9421 ggattggcag ctaaataatc agctaatgct tgccctaatc gtcctgtcga atgatttgta
     9481 atagcacgta cttgatcgat tttttcagtc gttcctccag ctgtgattag aatcttcatt
     9541 tatggtcacc tacatttact agtgttcttc cttggtgtgt tccagcaagt agtttagatg
     9601 ctgtttcagg caattgatcc agagtaatct cttggacgac cagttcatct gcaatctgca
     9661 aatccgccat ctgttgccat aaagaaagac gttgatctat tggtacattg acagaatcaa
     9721 ttccaatcaa ctggatgttt cgcagaataa aaggcaagac agtcgcgttt agtttgattc
     9781 ccccagcatt gccacataaa gctactgcac cgttataaga gatcaatgga agaagactgc
     9841 ttagctgctc gccacctact gtgtcgatga catagtcgat ttgctgtttt cctaaagctt
     9901 ttgttgtctc tggcagaaat tcatcaggtc gtacgacttg caaagcacct agacttttta
     9961 gccatgtgac agactcttct tttctggaaa aggtaatgat tgactggaat cctagtttat
    10021 gtaacagggc tatcgctgta ctgcctacac cgccagaagc accagtaacg acaacttttt
    10081 tatcggaggt cataccttga cggattaaag cgttcacaca caacatagca gtaaaccctg
    10141 ctgtaccaag gatcatcgct tgacgcagcg tcatattttt tggtagagga actaaccagt
    10201 ctcctggtac tttttgatat tggctataac cgcctggatg ggtgacgcca agcccaaaac
    10261 cagtagcaag tacaagatct tcttttgaaa aacgtggatt gttactttgg acgacagttc
    10321 cggataaatc aatccctgga gtcatcggat atgttcgtat cacctgacca gactcgctca
    10381 ttgccaatgc atctttataa ttgacatcag aataggcaat ttttaccaag acttcatttt
    10441 cagccaactc agtaaatggc agatcactga ttgctgtatg gaaaacaggt tcctttatta
    10501 cctgaaaaga tcgaaaagcg ttcattcgtg agacactcct tttaaaattt tcttttatca
    10561 taccataaag cctactcttt tattcttgtt cttccgtaaa tttagacaaa aattaggtga
    10621 aaaataaaga atcaaacaac tagaatttat tccaaaagat aaagaaatat tcagcaaaaa
    10681 aaattttaca aagggttgcc tttttcaata aatttcgtat actaatacga gtggtagtaa
    10741 acaggaagtt tactattaga aaacttcgat tcggaggaaa ttatggaaat tggtgagaag
    10801 ttaagaaatt tgcgcatcca aaagaatttg acacaagaag aactaggcga acgcacagat
    10861 ttgtcaaaag ggtatatttc ccagttggaa cgagatttaa gttcaccatc aatggaaaca
    10921 ttcttttcta ttcttgaagt cttaggtgtg actccagaag aattttttca tcaagaagca
    10981 gcgaatctgc aagtggttta ttcaaaagaa gatcatacgg tttatttcga tgaagaaaac
    11041 gggtatgaat tggaatggct cgtggctgat tcgaatgaaa aagaaatgga gcctgtcttg
    11101 ctgacttttg aaggtgcagg agaatataag acgtttgaac cttcattatc tgaaacgttt
    11161 atttttgtat tagaagggga attagagtta agcttaggag agacggtcta tgtggcaaaa
    11221 gcaggacagt cgatctatta tcaagcgaca aaacaacatc aattaaggaa tcatctcaaa
    11281 aaacgaacaa aagtattgat tgttgcaacg caatcatatt tgtaatatag ggggtaaaaa
    11341 cgtggggaaa ccaatcattt catttgatca agtagtgaaa cgctatgatg atgaaacagt
    11401 attaaaaaaa gtaagttttg agatcgaaca aggaaaattt tatacactgc ttggtccttc
    11461 cggctgtggt aaaacaacga tcctacgtat tatagctgga ttcaccgaag ctacagaagg
    11521 ggacatctat tttgaaggga aacggttgaa tgatttgcca gctaataagc gacaagtaaa
    11581 tacagtcttc caagattatg cactttttcc tcatatgaat gtttttgata acgttgcttt
    11641 tggattgaag atcaagaaaa tgtccaaatc agatatcgaa aagaaggtaa aagatgcttt
    11701 gcgtatggtc cagcttcctg gatacgaaaa acgggagatt agtgaaatgt ctggtggtca
    11761 aagacagcgt gtagcgattg cacgagcaat tgtaaatgaa ccgaaagtat tgctattaga
    11821 tgaaccgtta tcggcgcttg atttgaaact acgtacagat atgcagtatg aactgcggga
    11881 tctgcaacaa cgattaggaa tcacgtttat ttttgttact catgaccaag aagaagcgtt
    11941 ggcaatgagt gatgagattt ttgtcatgaa taaagggaaa atcgtccaaa gtggtacacc
    12001 cgtagatatt tatgatgagc caatcaatca tttcgttgct gattttgttg gggagagcaa
    12061 tatcgtggac ggtgtaatga tcgaagataa tctagttgag tttgttggta agcaatttga
    12121 atgtgtcgat ggcgggatgc gtctaaatga accagttgaa gttgttttac gcccagagga
    12181 tttgacgatc acgacaccag ataaaggaaa actagtcgta acggtcgata ctcagctgtt
    12241 tcgtggtgtc cattatgaaa tcatctgtta tgatgaacag caaaatgaat ggatggtcca
    12301 ttcaacgaaa aaagctaaag aaggaagcaa agtaggattg gcctttgagc cagaagatat
    12361 ccatgtcatg cgtttcaacg agtctgaaga agaattcgat gcacgtcttg acagctacga
    12421 agaatagggg ggacaagaat gaaaacaatg cgcaggatct attccgtccc ttatattttg
    12481 tggctcggtt tattcgtgat cgcgcctgta ttaatgatca tttatcaatc tttttttgat
    12541 atgaatgggc aattcacgct gaataattat ctatcttatt ttacgtcggg gacatcgctt
    12601 tctatgacga tcaattctgt atggtatgca tttttgatca cgtttttcac gctgatcatc
    12661 agttacccga ctgcttattt tttaacaaaa ctgaagcaca agcaattatg gctgatgctg
    12721 attattttgc ctacatgggt gaatctgctg cttaaagctt acgcatttat tgggattttc
    12781 agtgtgcacg gcagcgtgaa tcatttcttt gattttatcg ggatcgggcc gaaacagata
    12841 ttattcacag acttcagttt tctgttcgtg gcaacgtata tcgagattcc atttatgatt
    12901 atgccaattt tcaatgcatt ggaagagatg aatccttctt tgatcagtgc tagtagagat
    12961 ttaggcgcga atagctttga gaccttccga cgagtgattt ttccgttatc attaaatggt
    13021 gtgaaaagtg gtgtccaagc agtatttatt ccttctcttt ctttattcat gctgactcgt
    13081 ctgattggcg gaaatcgggt gatcacttta ggaacggcta tcgaacagca tttcttggtt
    13141 acacaaaact gggggatggg ttctacaatc ggtgtgattt taatcattgc tatgttcatc
    13201 gtgatgctcc tgacaggcga gaagaaaaaa ggaggtcgct cataaatgaa aaaatttaaa
    13261 tggtcgtatc tttatttagg gatcgtcttt ttattcttat atgcaccgat cttctatctg
    13321 attttttatt ccttcaatga aggaggaacg atgaacagct ttacaggatt tacttgggaa
    13381 aattatgaag ctgtttttga agatacacgt ttgatcatta tcgtattgaa tacgttcatg
    13441 ctggctttct tatctgcatt gatcgccacg atcatcggga catttggggc tatgggaatc
    13501 tattacacga aaaaacgacg tgcacggaca gcgttgttga gcatgaataa catcttatta
    13561 gtctctcctg atgttattat cggtgctagt ttcttgattt tctttacact actaggattc
    13621 ggattaggtt ttcagagtgt attgctttcc catattgctt tcagcattcc aatcgtcgtc
    13681 ttgatggtct tgccaaaatt gcaggaaatg aacgattcaa tggtcgatgc ggctagagac
    13741 cttggtgcta ataatttcca agtagtcaaa aatatcattt tgccttatct ttctccagga
    13801 atcatagctg gatattttat ggcatttacc tattctcttg atgattttgc ggttacattt
    13861 ttcgtcacag gaaatggctt tacgacgctc tcagtagaga tctattctcg tgcaagacaa
    13921 ggaatcagtt tagaaatcaa tgcgctaagt gcgttagttt tcttgttctc catgatattg
    13981 gtcattggat actactttat tagtaaagaa aaccgtccaa aacggctgaa gaaaaacaga
    14041 agagtacgcg agggggtggc acggctgcga tgaaaaaatt acaatctctg tttttaggta
    14101 ttctcatcat tattgcggtg ctgctgcttg gtgttcgtca acttgaaaaa acaagtggca
    14161 tgagtggagc aaaggtactt accatctata actggggaga ttatatcgat cctagtttga
    14221 tcacgaaatt tgaaaaagaa tatggataca aagtgaatta tgaaacattt gattctaatg
    14281 aagcgatgtt cacaaaaatc cagcaaggtg ggacaaacta cgacatcgct ataccaagtg
    14341 aatatatgat ccaaaaaatg atcaaagaaa aactagtact tccattagat cattcgaaga
    14401 tcaaaggatt aaataatatt gatccacgat ttttagactt ggattttgat ccagataatc
    14461 agtattcgat tccatatttt tggggaactt tagggatcgt atataacgat aaattctttg
    14521 atgcaaatca aattaagcat tgggatgatt tgtggaagcc ggaattaaaa gacagtttga
    14581 tgctgattga cggtgcacga gaagttatgg ggctttcttt gaatagttta ggctattctt
    14641 tgaatagtaa gaatatggca cagctgacag aagcagcaaa taaattgaat catttaacac
    14701 caaatgtcaa agcaatcgtg gcagatgaga tcaaaatgta tatgatcaat gaagaagcct
    14761 ccgtggcagt gacattttct ggcgaagctg ccgatatgat gtgggaaaat gaacatttac
    14821 attatgtgat cccaccagaa ggatccaatc tttggtttga taatatcgtt atgccaaaaa
    14881 ccgtgaaaaa taaagaaggt gcctatgatt tcatcaactt tatgctagaa ccggaaaatg
    14941 cggcacaaaa tgcagagtat attggttatt ctacaccaaa tgaagaagca aaagcgttat
    15001 tgccaaaaga catttcgtcg gacgaacaat tctatccatc tgatgacacg atctcccatc
    15061 ttgaagtcta caaagatcta ggatcgaaat acctaggcat ctataatgac ctattcttag
    15121 aattcaagat gtataggaga taatggcaaa actcagtaag aatggggact tatcaacgtt
    15181 ctaaagagct aaaaatcgtc ctttttctat ctgagttttc caatcttcta ccaaaagcgg
    15241 atgaaacgcc gctttttttg tgttatttta ttttttctac cacgtttttc taccagagaa
    15301 aagtggacaa aaccattgta aaatagggcc cgagacgggc tagtaaatct aagattgaaa
    15361 aagttacaga aggataaagt aattggaaac tatgcaaata ta
//
LOCUS       DAJSKS010000061        15336 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJSKS010000061 DAJSKS010000000
VERSION     DAJSKS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.062"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            133..3360
                     /gene="fss3"
                     /locus_tag="ON396_001588"
     CDS             133..3360
                     /gene="fss3"
                     /locus_tag="ON396_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HCR3113677.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3473..3787
                     /locus_tag="ON396_001589"
     CDS             3473..3787
                     /locus_tag="ON396_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR3113678.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3800..4174
                     /locus_tag="ON396_001590"
     CDS             3800..4174
                     /locus_tag="ON396_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR3113679.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4184..4519
                     /locus_tag="ON396_001591"
     CDS             4184..4519
                     /locus_tag="ON396_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HCR3113680.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4602..5942
                     /locus_tag="ON396_001592"
     CDS             4602..5942
                     /locus_tag="ON396_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR3113681.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            6020..6694
                     /locus_tag="ON396_001593"
     CDS             6020..6694
                     /locus_tag="ON396_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCR3113682.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6687..6851
                     /locus_tag="ON396_001594"
     CDS             6687..6851
                     /locus_tag="ON396_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113683.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            6927..8069
                     /gene="dcm"
                     /locus_tag="ON396_001595"
                     /pseudo
     CDS             6927..8069
                     /gene="dcm"
                     /locus_tag="ON396_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            8059..8349
                     /locus_tag="ON396_001596"
     CDS             8059..8349
                     /locus_tag="ON396_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113684.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8608..9792
                     /locus_tag="ON396_001597"
     CDS             8608..9792
                     /locus_tag="ON396_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR3113685.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9789..9926
                     /locus_tag="ON396_001598"
     CDS             9789..9926
                     /locus_tag="ON396_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCR3113686.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            10672..12582
                     /gene="ltrA"
                     /locus_tag="ON396_001599"
     CDS             10672..12582
                     /gene="ltrA"
                     /locus_tag="ON396_001599"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR3113687.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            12686..12910
                     /locus_tag="ON396_001600"
     CDS             12686..12910
                     /locus_tag="ON396_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113688.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            12923..13426
                     /locus_tag="ON396_001601"
     CDS             12923..13426
                     /locus_tag="ON396_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR3113689.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            13486..13875
                     /locus_tag="ON396_001602"
     CDS             13486..13875
                     /locus_tag="ON396_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3113690.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            13862..>15336
                     /locus_tag="ON396_001603"
     CDS             13862..>15336
                     /locus_tag="ON396_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3113691.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGG"
BASE COUNT         5149 a         2545 c         3412 g         4230 t
ORIGIN      
        1 ttaaattttt aggctcattc attctcaaat ccattctatt cagtcaataa aaattttaaa
       61 aagactttat aaacaaagat gttcaagcga gcatcttatt tttttgtgct taatttgaaa
      121 ggagaacatt tcatgtataa aaaacaaaaa aagaaattat gtgtattaac tattttgagt
      181 ttggctgttc aagcaattgt tggagcaatt gctcctacga ttacttttgc tgatgaaatt
      241 acccatccac aaacggtaac cgtagaacta gatttagcac atcaatatgc tgttgaagga
      301 acttttagtg atggtcgtcc aatgtcagaa gtcactgttc cgcattatgc agtgtacaac
      361 ggctttaaac aagatatttt ctgtattgaa ccaggagtcc cgatttacaa tgagttcact
      421 cctggctatg agaaaaaccc gttaccagat atgtccgata aagccaaatt agtttccgtt
      481 ttatggaaaa aagtaggtac agatgcagat actcatattg tggcacagaa gatgatctgg
      541 caagaagtta atgggtatac ccttcactca ataaaacgat cagatggtag tgccgtaaat
      601 atcgcagcca ttgaggcaaa aataaatcaa gccattgacg attatcagaa gaaaccaacc
      661 ttccataata gtacggctaa aacagtatta gggcaatcga ctactgtgac ggatacgaat
      721 aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg ccgcaaacat tgattatcgt
      781 gtgaatggta atcagttgat aatcacccca aatgccaatt caaaagaaag tggtgtgctg
      841 acgcttaaga aatctgctgg taccggaaca cctgtcgcct ataaaaaggc tggccaacag
      901 actttaatgg ccggggcgat tgataaacct aatacctaca caatcaaaat tgatgtggaa
      961 acggagggtt ctttaaagat caaaaaagtc gataaagaat cgggtgatgt tgtaccagga
     1021 acggttttcc atttagactt tggaaaaact ttacctgcaa aagacgtgac tactgacaaa
     1081 gaaggcattg ctacattgga tgagattcct catggtacaa aagtaaccat taccgaaaaa
     1141 tcagtaccgg cgccttatac gattgatacc acccccatga ctaccaccat taaagcaggc
     1201 gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag ggcaaatcat tctggataaa
     1261 agcggagttg aaacagggag tgatctttgg aatgacaatt attccttagc tggtaatacg
     1321 tttgccattc gcaaagacag tccaaccggt gaaatcgttc aagaaatgac cacggatgaa
     1381 aacggtcatg cggaaacacc aaaagagatt gctaatgcgt tggaattggg aacctattat
     1441 gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt tcaaacctgt aaaagtcgaa
     1501 ttgaagtatg ccaatcaaac cgtagcccct gttaccagta acgtaaaagg gcaaaatcaa
     1561 gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag atactgggga taaagcacaa
     1621 ggtaaggcag tctttgaggg tgctgaatac accttgttta ctgctaagga tggaaaagct
     1681 gttaaatgga gtgaggcttt taaaccagag ttagcgaagg gaacgaaagg ttctgatgaa
     1741 acggtcactt tgactttaga cgaaaagaac caagttgccg ttaaacattt agctatcaac
     1801 gagtattatt gccaagaaac aaaagcgccc gaaggatata gccttgatga aacaaagtat
     1861 cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg ccgtgattac ccgaaatgtt
     1921 acggcaaaag aacaagtgat tcgctttggc tttgatttct ttaaatttgc tggatcagct
     1981 gcgggcacta ccgaaactgg cttcaatgat ctgaccttta aagtgtcgcc attggaaggg
     2041 accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag cttataacga gcaattaggg
     2101 tttgatggtt atggcaagtt tgaaaatctt ccttatgggg attatttact tgaagaagta
     2161 gaagccccag aaggttttca aaagattacg ccattagaaa tccgttctac ttttaaggaa
     2221 aacaaagaag actttgtgaa gagtgagtat gtctttacca ttaccgaaca agaccaaaaa
     2281 caaccaatta aaacggtgac cgttccttac gagaagctga cgaacaaagc attttctgtt
     2341 agtttgaacc gtttaatgct ctatgatttg cctgaggaag aagatagttt aacttctctt
     2401 gcgacttgga aagacggaaa caaagaatta acttctctgg attctaccga gctagttgat
     2461 aaattgagtt acaatctaca tgaaatcaaa gaagactggt atgtggtagc gcaagccatt
     2521 gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa aagccaaacc agtggtgatt
     2581 gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag gaacttggaa aattcagcat
     2641 aaattaaccg ccgaacaagt tttaaataaa actattgtct tattcaacta tgtgtatgaa
     2701 aataaggaag cttttgaagc aggcgataag ccagtagcga aggatgttag cttgaataac
     2761 caagcccaaa ctgtcagttg tacagtggaa caccatgttt ctatccaaac aaaggcccac
     2821 ctagaaaacg gttctcagac ctttactcat ggtgatgtgg tggatatgtt tgatgatgtg
     2881 tcgattactc atgatgtact ggatggctca aaagaagctt tcgaaacaat tctgtatgct
     2941 ttattaccgg atggtacaaa caaagaaatt tggaaatctg gcaagattga ttatgaagta
     3001 aatgataaag aattcaccaa aactgtgctt gcgaaaaaag tagataccgg aaaatatcca
     3061 gaaggaacca agtttacttt tgcggaaatc aattacgata aagatgggaa tatcaatgga
     3121 aaacacaatg aagatttgaa agaaaaatct caaaccttaa caccaaaaga agttccaacc
     3181 atactaagta cgccgaaaca accggaaaca ccaactgttc caagtggctc tcaagaatct
     3241 agtcccacag tgaagacctt ccctcaaacc ggggagaaaa attccaatgt tttactgttc
     3301 attggtttta ctttgatttt tgcgacggcg ggctattatt tctggaatcg ccggaactaa
     3361 ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg cgcgaccgcc tcctaaaaaa
     3421 gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa gaggaaaaga agatggaatt
     3481 gaaatttgtc gtgccagata tggctgaaac ctttggaaag ttaagttatg ccggtgaagg
     3541 agaaattata acagaaggat atggacggaa tactacggtg attggtcgca gttatcattt
     3601 gtattccagt aagcaacggg ccgatgatat tgaggtggta gtagctgcgg aagccggtga
     3661 aaaggatttt gatcaagacc aaccgctaaa agccgtgaat ccccatttgg ttgccaaagg
     3721 ctatgagatt gagaatcgtg ggtttactga ttacgtgttg tatgttgatg atttagtgaa
     3781 agcataggag gaaaagaaga tgagattaac agaaggtatt gtcgtagatt caggtttaac
     3841 atttgggaag ttgcgttttt ccgcattacg ccgagaagta cggaagcaaa atgaggatgg
     3901 aacaattggt aatgaggtaa aagaacgaac ctataattta aaatcctctg cacaagggcg
     3961 aatgattcaa gttagtatcc cagctaatgt acccttacgc gagtttgctt atgatgcaga
     4021 ggtagagttg gtcaatccaa tcgtggatac cgttgccaat tatgtttttc gagaaggaac
     4081 taccgttaat tggttcatta aggcggatga tttagtcttg aagcggcagc cgaatcaagg
     4141 aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag attatggaaa ttaatgttga
     4201 acatatttta gattgtctcg atcaatatgg aaaaggcgag ctgactgagg aacaactcgt
     4261 taaagcatta acctctgatg aaaaaatgtt tctgattata catcaaggtt tacttggaga
     4321 aaatagcgac agtgaagaag actctgatgt actagactgg ttaggagaag aagagtcttt
     4381 ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag gcagaatcca ttttggaaga
     4441 aattggtgtg gagatgccgg atgctattga aggcttttta aatcaattgg ttgaaacgaa
     4501 acaactcccc tcctcgtagc agtaaatgac tagcataact ttttgaaatt gcctaatgaa
     4561 atcctattgg gctttttcga ttggaggtgg aaaccatcaa aatgtacaaa ggacatcgca
     4621 tacgcgcagg agatcagcac ttggtttatc actttgttct tggctggcta ctcgctctgt
     4681 tcatcggttg gatgagcgtc ttttattttc aagagtttag acaatttgat atttctaagc
     4741 tatcactttc tactattgaa atcgtttggt ctataaaaga tttagtatgt ctgcttggaa
     4801 gtctcgcttt ttcaggagct atgatattgc tttatattca cttttttctt gatcattggc
     4861 gaagcttgtg gcatcgacaa aagctagctc ggatgattct agagaaccat tggtatgaag
     4921 tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa tagtagccga accagagaga
     4981 ctatcagtta cttccccaaa atctgttatc gaatgaaaga tggattactg tctattcgcg
     5041 ttcaaatttc acttgggaaa taccaagatc agcttttaaa attggaaaag aagttagaaa
     5101 gcggactgta ttgtgagtta gtagaaaagg aactcaaaga ctcctatgtg gagtatactt
     5161 tactgtatga tatgatcgcc aatcgaattg ggatagacga agtagtggca gaaaatggga
     5221 ctctgcgatt gatgaaaaat caagtatggg cttatgattc tttaccccat atgttaatcg
     5281 ctggtggcac gggtggaggg aagacctatt tcctcctcac cattatcgaa gcactattga
     5341 agtcagatgc agaattgttt atcctcgatc ccaaaaatgc agatttagct gatttaggta
     5401 cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc tgcttgtgtg gaagatttct
     5461 atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat gtctaactac aaaacaggag
     5521 agaattatgc ctatcttgga cttccaccca actttttaat ctttgatgaa tacgtggctt
     5581 atatggagat gttaacgacg aaagaaagtg cggtgatttt gaataagctc aaacaaatcg
     5641 tgatgttggg tcgtcaatct ggtttctttc tgattttggc ttgccaaagg ccggatgcga
     5701 aatatttagg agacggaatc cgcgatcaat tcaactttcg cgtggctttg ggtcgtatga
     5761 gtgaactcgg atattccatg atgtttgggg aggtcgaaaa aaacttcttt atgaagcaca
     5821 tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat tagtgagttt tatacgccac
     5881 ttgtaccaaa agggtatgat ttcttagatt caattagtaa attagaaaag atgaatgatt
     5941 gagtgatctt caatactgac tttatacttc aagtaacgta taatactttt atatagtagg
     6001 aagaaagggg ataattaata tggaaagtaa tgattcttat ttagagtatg caaaagcaaa
     6061 taaggataag tttatttctt atattatcaa agggaaatcc cccgaagttg gacgagacgc
     6121 agtttttatg gcagggagtc ctggggctgg aaaatcagag gttgccattg gattagctga
     6181 gaattatgat aaccatgtga ttattgatgc ggattacttt cgcacccagt ttcctgatta
     6241 taatggaaaa aactctagtc tctttcaaaa agcatcttct tggttggtag agcaggcatt
     6301 tagatatgtc ttagatcatg gctattcttt tattctggat gcaacttttg caatattaag
     6361 tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt cgagtgacga ttttctatgt
     6421 ttatcaagac cctaaaatcg cttggaaatt tacacaagaa cgtgaaattg cagagggacg
     6481 tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa tcaagagaga acattgcaaa
     6541 agtaaagagt aaacatccag atgtattgct tcatatcata ataaaagatt atacgaataa
     6601 tatttccgaa gttcattatg ctacggataa tctagaatta tttttaccac tagggtatag
     6661 taggaaagat ttggaggttc aattacatga ctgagaaaga acaagtaacg aaaattgtaa
     6721 agaaatacaa taagagcatt gcggatttat cggaaaatgc tactgcaaaa gagttcaaaa
     6781 cggtgattaa atatgtagcc gatcaggcca atgaaaaaca acggaaatta gttggattaa
     6841 ataaaaaata gtaggacaaa ttaataatgt atgagccaag agcaaaatgt tcttggcttt
     6901 tttctttgca atgaatgagg taatgtatga gatttttaga tttattcgcc ggtattggtg
     6961 gctttcgttt aggtatggaa caggcgagcc atcattgtat cggtttttgt gaaattgata
     7021 aatttgctag acgtagttat aaagcaattc atgatacaag taaggaggtg gaaatgcatg
     7081 acatcacaag tgtatcagat gagtttattc aatccctcgg accagtggac atcctttgcg
     7141 gcggatttcc gtgccaagct ttttcaattg cgggaaagcg gcaaagattt tccgatactc
     7201 gaggtacttt attctttgaa atcgctcggt tcaccactct tctccgacct aagcttttat
     7261 tccttgagaa cgtccgagga ttgctcaatc acgaaggagg ggctacgttc gagacgatcc
     7321 tccgaacgct ggatggattg gggtatgacg tggagtggta ggtgcttaac tcaaaggcct
     7381 acattcccca aagccgggag cggatattcc ttatcggaca ttctcgagac gcatgtaccg
     7441 aacaagtatt tcctatcatt ggaccgtctt caacatctga tcaaaacatc agaaatttgg
     7501 taaatatcaa tccttccaat cgaggaatgg gtggacaagt ctatggttca gatggcgtag
     7561 cgccaacctt aacaggtgat gaaggaatca aaatcgcact gccagcaagg gatggaatct
     7621 ctgtagcagg actattaccg ggaaagtttg aacaaggtaa tcgagtttat gaagttactg
     7681 gaaccgcccc caccttgtca acgaaacaag gtggtacaaa aatcatgatt cgaaaaaatg
     7741 aaacagaata tcaagaggtg aagccagaag acagtgtgaa tctggctttt cctaattcca
     7801 caagtcgccg aggtcgtctg ggaaaacaga gtgttcatac cttactaaca ggagaccaac
     7861 aagcgatcgt cacggatcag tatcaaattc ggaagctaac acctagagag tgttggcgac
     7921 ttcagggctt tcccgattgg gcttttgatc gagattctca agtcaattca gatagtcagt
     7981 tgtataagca agccggaaac tctgtaacag ttccggtcat ttttgatatt gccagaagat
     8041 taaaagagtt ggatgtacat ggtttatgat gagttgattg gagaaattta ttgggtgatt
     8101 ggaaagattc agtcagaccc agaattagaa gaacaattgc gacggttaaa atttgatatt
     8161 cgcaagaatg gtgtcaaagt cgctggcgat ccatatttaa tgaatgagga gacggatgct
     8221 cgaattgaaa taaatcaagt aattgcagaa tttgagcgga ttgcggacca agcaaaagaa
     8281 ccagatattc gtcaatactt gttagaaatg aaagcagagt tggaagtaaa tggtattaca
     8341 gatgagtaac ctaaaccaac acaactgact gttaggcgct gaccgcggag gattcgcgaa
     8401 gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc gttagccgac catgatgtct
     8461 cacccccgta tctaacaggg gggtacaaaa acacaaaaaa taagtaagca accattaaga
     8521 atgcttgttg tatcaaagat agtaagtaat ttgtataggt gttaactcat ttgaaaaagt
     8581 taacaccttg aatttggagg tgagaacttg gcacaacgga atttagatta tcggctatta
     8641 aaagaccgac gcaacgaata cggcgtttca caaaataaat tggctacggc ttgtggactt
     8701 agtcggccgt acttaaatca gatagaaaat ggtggcgtaa ccgcatctat aaaaaccatg
     8761 aggaaaattt ttgaccaact ggaaagcttt aatcccgatt tgcctttgac gttactgttt
     8821 gactatgtaa ggattcgctt tcccacaacg gatgcacgaa aaattattca agaaattctc
     8881 catttgaaat ttgagtatat gctccatgaa gattatgcct tttactccta tcaagaacag
     8941 tatgtcatgg gtgatattgt ggtgatgttg tctcatgaag aagataaagg tgtgctttta
     9001 gaattaaagg ggcgaggttg ccgacaattt gaaacttttt tactcgctca aaagcgtagc
     9061 tggtacgact ttttcgagga ttgtctaaag gccggtggcg tgatgaaacg cttggacttg
     9121 gcaatcaatg atcgagtggg gctattggat atacctgagc taacgaaaaa atgtcagaag
     9181 gaagaatgta tctccttgtt tcgtaccttt aaaagttatc gttccgggga acttttaaag
     9241 gctgatgaaa aggacggcat gggcaatacc ttatatatcg gcagtctcaa gagtgaggtt
     9301 tatttctgtt tgtatgaaaa agactatgaa caatatatca agttagggat tccattagac
     9361 caaactgaga cgaaaaatcg ttttgagatt cgactgaaaa atgaccgtgc ttttcatgcg
     9421 attcaagatt tgttaaaagg tcgtagcatt gagagcacca cgttttccat tatcaatcgt
     9481 tacttgcgat ttgcggataa agtagaaggt aaaaggcgaa ccaactggcc cttgaatgaa
     9541 caatgggggc gttttattgg acggaatcgg aaagagattc aattaacttc cgagccaaaa
     9601 ccgtatacca ttgaacgaac cttgaattgg ctaggccgac aagtcgcccc tacgtggaaa
     9661 atggctaagg agttggatcg attgaatcgg acaacctata tccaggatat ggtacagaat
     9721 gcacgcttat cagaccgaca taaaaagatt ttggagcaac aaagtatggc aattgaaaat
     9781 ttgattatat gaaaagagga atcgtcatgg gaattgtaaa agatattttt ttggtatttg
     9841 gtggcagtat gcttggtgtt actgtgatgt gcttgatgaa tgcaagtgca gcagcggatc
     9901 gagcaatgga aaagaaagga aagtgacaag atgaatttcg gacaaaattt atgtgcgact
     9961 cgttccttag tgaaaagcta aggcactaat aaaagaacga aaaagtttgg ttattaggag
    10021 aactgataat ctgacaggtg gaatgatgaa gtaacgctct gaaacgcctg ccctgagcct
    10081 ccgacaagca ttgtatgagg aaactcagaa tgtttgaagc tcggtaaaga cggctgaaag
    10141 ataccgtaat agttgatgaa catatatctt ctatcgaaaa ctatccagag gtgggtagcg
    10201 tatccaacag gtcgggggtc tataaaatat ctatggttag aatgttctat aaggaactga
    10261 cgaaacagcg aatgtacggg tctaaatttg aactttgtag aaatgcaaag gtacgagaaa
    10321 tcgtagctgg tgtggcaaag taagaatata ggttatgaaa tgccatataa cgttacaggc
    10381 ggtatcaagc cagcaggctt atagctagtg acctaaggat atatatgtat agataagatt
    10441 atcggaacga ggaaaggtat tgggttctca tcaagagaat aaactgacga agaacaataa
    10501 gcagttgaac taatgctgaa aagcagaggg cgaacctaag aaattcttgt aatgagaatg
    10561 gaggaatgcc ctcaagtcgt gtatatatta gaaaagtatt atgttgtttc aataaggatt
    10621 acgagtaaga taaaaaagct cactcaattt caagtaaagg agtgaagcct tatgcctaaa
    10681 aataaaaatg aaacattgtg tgtagaagat ttaagacacg ctgaatatta cgaaatgcaa
    10741 aacacctttg atgatttata tgccaaaagt aagaatggag atattttcac tcatcttatg
    10801 gatattatcc tatcgagaga aaatatatta ctggcatatc gaaatatcaa agcaaatgca
    10861 ggcagtaaaa cagcaggaac agacggtaca ataattaaag atattggcaa attgccagca
    10921 gaaacagttg tcaaaaaagt aagatacatt gttgcaggca gtccacatgg gtatcgacct
    10981 aagccagtga ggcgaaaaga aataccaaaa ccaaatggta aaaccagacc tctaggtatt
    11041 ccttgtatgt gggacaggct tatacaacaa tgtataaaac aagtattaga accaatctgc
    11101 gaagctaaat ttagtgaaaa cagctatggt tttagaccta ataggtcagt tgaaaatgca
    11161 ataaaagcaa cctataaccg cctacaaatc agccagctac attatgtgat tgaatttgat
    11221 attaagggtt tctttgacaa cgtgaaccat tccaagctga taaaacagat atgggctatg
    11281 ggaatacggg acaagcactt gatattcatt ttaaaaagga tattaaaagc tccaatcaaa
    11341 atgactaacg gtacaataac atatccagag aaaggaactc cgcaaggggg aataatatcc
    11401 ccactactag caaacattgt tcttaatgag ttagaccatt gggtagaaag ccaatggcag
    11461 gaaaatcctg ttacaaaaaa ctatgtggtt catatcaata aaagcggtag cccatgtaaa
    11521 agcaacgctt acaaagaaat gaagaaaaca aagttaaagg aaatgtatat ggttcgttat
    11581 gcagatgatt ttagagtttt ctgccgctac aaggaaagtg ctgaaaaagc caaaatagca
    11641 atcactcaat ggattgaaca acgattaaaa ttggaagtat cacaagaaaa aacaagaatt
    11701 gtaaatgtta gaaaaagata ctccgacttt cttggtttca agattaaaat gataccgaga
    11761 agaaagaagt tagttgttaa atcacatata agtgataaac aattcaaaaa ccaaaaagac
    11821 aagctggtat cacaggcaaa gaaaattgct tctccctcaa aagggaaaac ggaattagaa
    11881 gaaacaagac tttataattc aatggtatta ggtatgcaaa attattatca gattgcaacg
    11941 aatgtaaatc tggattgtat gaaactgaat agagctgtaa tggctatctt cactaacaga
    12001 ttaggtacaa acaaaagggg cagattgaga aaaacaggca gacaacttac gacaaaggag
    12061 gcaacaagat atggtaaatc taaaatgctt cggtatgttg ctggcgaggg cgaacctatt
    12121 ttccctgttg gatatatcca acacagaaaa ccaatggcaa aggtgtattt agcgaattgc
    12181 tacactctag agggaagaaa atttatccac accaatctaa gtgttgataa attactgatg
    12241 taccagttaa tgaaattatc tttggaaaat cgcacaatag aatacgcaga caatcgaatt
    12301 tcgctatttt ctgctcaaca tggaaaatgt gctataactc tggaagaatt tcaacaagct
    12361 aatgaaatac attgtcatca tattgtacca actggagctg gtggaaatga cgattataaa
    12421 aatcttattt tggtaaaaga ggcggtacac cgtctaatac acgctaaaac agaagaaaca
    12481 atacaaaaat atatagctct attgaagcta aataaagtcc agctcataag actaaataaa
    12541 tatagagtat tagctggcaa tcaagaatta aacttgatat aggtctacta cataaatact
    12601 tttcaatata aacacgatgg aacgcggagt gcggtgaaag tcgcacgctc cgtgtgaatg
    12661 gggggaaaag tttgagatat ttataatatc aggaacttac ctatccatat atcaatggtt
    12721 tttaaccaat gcccaatcct tagtcttact ggcaattgtg gtcattggtt tgtttttagg
    12781 atttaagcgg gagttttcca aactgattgg ctttttggtg attgctttga ttgcggtcgg
    12841 attggtcttt aatgcttctg gggtgaagga tgtcttactc agtttgttta accgaattgt
    12901 cggtgcgtaa gggggaagca cgatggaaca gatgcgtgtt tatattgcga acctcgggaa
    12961 atataacgaa ggtgaattgg tgggggcgtg gtttacccca cctgtcgatt ttgatgaagt
    13021 gaaagaacga attgggttaa atgacgaata cgaggaatat gcaattcatg attatgaact
    13081 tccctttgag attgatgagt atacgccaat tgaggaaatt aatcggttgt gtggcttagc
    13141 ggaggaactt gaaggaacgc cagttggtga agttgcttct gagattcaac atgctttctt
    13201 caattctttt gaggagatgg tagagcatgt ggatgatatt atctattatc cagattgcga
    13261 tgatatgagt gatgtagcat atttcttgat tgtagaagct ggtgattttg gagaagtacc
    13321 agaacacttg aagacgtata ttgattttga tgcgtgtggt agagacttgg agattggagg
    13381 caattatttg gtaacgagtc gaggtgtttt tgagtatcca tattaacaaa attattgaag
    13441 ggtagttgat ttctaaacta tccttctttt taaaggaatg atttgatgaa gaaaataaaa
    13501 agctacacca gtatttgggc agttgaaaag gtcatttatg caattaatga ctttcaactg
    13561 ccttttcctg tgacttttaa ccagatggct tggtttgttt tatccttgct gtttgtcatc
    13621 gtatttgccc atgtgcctcc actatctatg atagaggggg cctttttaaa atacttaggt
    13681 ataccggtgg cagttacttg gttcatgtcg caaaaaacgt ttgacggtaa gaaaccattg
    13741 ggatttttac gatcttttat tagctatcac ttacgattta aggtcacttt cgcaggaaag
    13801 aaagtcaaag aacagaagaa acgatgggac gaacccatta ctctggtaag gagtgtgaac
    13861 tatgtacccg attaagtata ttgaaaataa tcttgtcttt aatcaagagg gagaatgttt
    13921 tgcctactat gagttaatac cttacaatta ctcctttttg tcacctgaac agaaatatca
    13981 agtccacgat aactttcgtc aattgattgc gcaaaatcgt gaaggcaaga ttcatgcctt
    14041 acaaattgcc acagaaagta gtattcgtgc cacgcaagaa cgatcaaaaa aggaaattac
    14101 cgggcgatta acagaagtag cgaaacaacg aattgattta cagacagagg cattagtatc
    14161 catgattggc gatagtcaga ttgattatcg cttttttatt ggcttcaaac tgattgcgac
    14221 agatgaagag gtcaatctaa agaatctcaa gaaatctttc ttctccggct ttcaagaatt
    14281 tgtctatgga gtgaatcatc atttgatggg cgattttgtt tccttatcca atgaagaaat
    14341 tcgccgatat accaagcttg aaaaactaat ggaaagtaaa ttggctcgaa gatttaaagt
    14401 cagacgagtc acaccgagtg atctgatgta tttgattgaa catatctatg gcgaaaaggg
    14461 tacaccattt gaagaatatg aatttcagct accaaagaaa aagctgaagt cggaaacttt
    14521 ggtgaaacgt tacgacttat tacgtcccag tcgttgtttg attgaagaaa agccccggta
    14581 cttatgcatg gaacatgaga atcatgaatc atatgtagcg tacttaacaa ttaatacaat
    14641 tgtgggggaa atggagtttc cctcctcaga acttttctat taccagcaac aacaatttac
    14701 attcccaatt gacactagta tgaatgtgga gattgtgacg aacaaaaagg cacttgctac
    14761 cgttcgcaat aagaaaaagg aattaaaaga cttggacaac catgcctatc aatctgacaa
    14821 tgaaacaaat tccaatgtct tagatgcatt ggattccgta gatgaattgg aaactacctt
    14881 ggatcagtcc aaagaatcca tgtacaaact gagctatgtg gttcgagtaa gtgccgaatc
    14941 agtagatgaa ctaaagcgcc ggtgtgatga agtgctagat ttttatgatg ataccaatgt
    15001 aaaactagtc cggccttttg gcgatatgat ggggctacat gaagaattct taccttctag
    15061 taagcgatac atgaatgatt atatccagta tgttacgtct gattttctcg ccggacttgg
    15121 ttttggcgcc actcagatgt taggggagtt ggaaggcatt tactttggtt ataacgtaga
    15181 tacggggcgc aatgtttacc tgaaaccggc attggcctca caaggggtca aggattcagt
    15241 taccaatgcg ctagcagcgg cattcctcgg ttcactcggt ggcggaaagt cctttagtaa
    15301 caatctctta gtttattacg ccgtcctttt tggtgg
//
LOCUS       DAJSKS010000062        14625 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJSKS010000062 DAJSKS010000000
VERSION     DAJSKS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.063"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            4..816
                     /locus_tag="ON396_001604"
     CDS             4..816
                     /locus_tag="ON396_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCR3113692.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="ON396_001605"
     CDS             813..1613
                     /locus_tag="ON396_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR3113693.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="ON396_001606"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="ON396_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCR3113694.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="ON396_001607"
     CDS             3110..4819
                     /locus_tag="ON396_001607"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR3113695.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="ON396_001608"
     CDS             5031..9389
                     /locus_tag="ON396_001608"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HCR3113696.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="ON396_001609"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="ON396_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR3113697.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="ON396_001610"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="ON396_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR3113698.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="ON396_001611"
     CDS             11227..11520
                     /locus_tag="ON396_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCR3113699.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="ON396_001612"
     CDS             11517..11828
                     /locus_tag="ON396_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HCR3113700.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="ON396_001613"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="ON396_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR3113701.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="ON396_001614"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="ON396_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR3113702.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4983 a         2598 c         3255 g         3789 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaatc aggctctttg tcaataagga ctgatgagtt
    14581 gtgcaaaatc aaatctgagt cagaatgaac cacattctgg ctcag
//
LOCUS       DAJSKS010000063        14577 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJSKS010000063 DAJSKS010000000
VERSION     DAJSKS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.064"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..571)
                     /locus_tag="ON396_001615"
     CDS             complement(<1..571)
                     /locus_tag="ON396_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR3113703.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGT"
     gene            complement(819..1655)
                     /locus_tag="ON396_001616"
     CDS             complement(819..1655)
                     /locus_tag="ON396_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HCR3113704.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1666..2595)
                     /locus_tag="ON396_001617"
     CDS             complement(1666..2595)
                     /locus_tag="ON396_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR3113705.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2787..3140
                     /locus_tag="ON396_001618"
     CDS             2787..3140
                     /locus_tag="ON396_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR3113706.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3214..3870)
                     /locus_tag="ON396_001619"
     CDS             complement(3214..3870)
                     /locus_tag="ON396_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCR3113707.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4096..6030
                     /locus_tag="ON396_001620"
     CDS             4096..6030
                     /locus_tag="ON396_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR3113708.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6116..7339)
                     /locus_tag="ON396_001621"
     CDS             complement(6116..7339)
                     /locus_tag="ON396_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR3113709.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7333..9231)
                     /locus_tag="ON396_001622"
     CDS             complement(7333..9231)
                     /locus_tag="ON396_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HCR3113710.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(9225..10343)
                     /locus_tag="ON396_001623"
     CDS             complement(9225..10343)
                     /locus_tag="ON396_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR3113711.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10426..11259)
                     /locus_tag="ON396_001624"
     CDS             complement(10426..11259)
                     /locus_tag="ON396_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3113712.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11252..12169)
                     /locus_tag="ON396_001625"
     CDS             complement(11252..12169)
                     /locus_tag="ON396_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3113713.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12396..13520)
                     /locus_tag="ON396_001626"
     CDS             complement(12396..13520)
                     /locus_tag="ON396_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3113714.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13703..>14577
                     /locus_tag="ON396_001627"
     CDS             13703..>14577
                     /locus_tag="ON396_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3113715.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTG"
BASE COUNT         4603 a         2883 c         2352 g         4739 t
ORIGIN      
        1 ttgtgccgtt tacgagaatc tcttcatctt tcaatactag gcctatttta tttttataac
       61 ctggcatgct cattagataa aggatttcat cgtaatggat atagtgatcg ctattaccat
      121 aacggacagt tgtccacctt tttttcgtag catgttgaac aggatagttt tctttttctt
      181 tcaataagta actttggatg ctttttttat ttttttcata agtgaattct tttatcaaat
      241 aaccactagg cgtaatcttt cgattaataa cagttattgc tttattcgga tgagacgtta
      301 gataaacaat tttacacttt tcattctttt ctctaagcat ttcgcctagt tcgataccgc
      361 tataataggc gttcaaatcg atgtctagga aaaaaatatc tgaggaatag atttcattat
      421 ttttaatgaa ttcaacaaat tgtataacgt tcaatccctg aacgatctca aatttccaag
      481 gacttaataa aattctggat tcttccaaaa tttctactag cattttttca aaaaccaaac
      541 tatcatccaa aataaatata cgcgttacca taaaaagtac ctccactcga tattttacta
      601 tcaaaatcat ttcatacaac ttttcaacat acatgttagg aataaattgt aattctcttc
      661 acaatctttt ccaacacctt tgaaaccgat atcaaatact ttcaggatgc tgcttctaaa
      721 ataaatatca aacaaatgaa tacatcatgc aaaaaaagcc agacaaccga cgtccttgcg
      781 tgtcagctgt ctgagctatg tttatttatt ttgttctttt aaaaagaacg atacacatac
      841 tgatttttcg gagcagagac tttctcagcg tttgtaacaa ctacagatgg gatttcacgc
      901 ttgaatggtt gataatagtc tgattggatg gttccttcta ttttatacca ttcatcattt
      961 ttaaccgtta ctccttccgg catatgcgtc aacaaaccaa agactcctga atctgccaca
     1021 caatggataa tgccgaatcg gaacaagaag atatctgtat tgctatcgtt cggtgtttgg
     1081 taaacaaatc cttcatagct gatttttttg ccaatgaatt cgctagggta attataaatt
     1141 agctccataa cttctaaata gttttcatct gtgatcttga tagtttgctt atccccatac
     1201 ttttctttca actcattcat ttggtctaag tagtctgatt tgttgaaata aatactggta
     1261 tccggcttta agtattgtgt ctgcatctcc ggatccccta ctgattcttt acttactggg
     1321 aaattaaatc ctttggcttc tacgatcgta gtgtcgagac taactgttgg aaacaacgtc
     1381 cccacgatta ctggcaaagc tagcagtaca taagcgattc cacgttgata aggttttgac
     1441 aacccatggt catgatcgtg ctcgtgtgcc ttttcttctg cttttccgtt tttgacccac
     1501 aaatacagct gaactaaagc caaaagagca gataaaatca ttgaaagaat agctaagtag
     1561 cgataatgga cattgatata ttgatcgagt ttgccagaaa cttgcaagta catcatcaat
     1621 tcgaaatacc cagataaaat caaaaaccgc agcatatttt ccctcctaaa taaataatgt
     1681 gaatccaata acgctaaggg caatcagtcc gatcagcgat aaaataaagc gtcctgtaaa
     1741 atatcgtttc atcatcagta gatttttgat atcgaccatt gggccaaaga ccaaaaaggc
     1801 agctacaggt gctgtgccga acagacttaa caaagaagat cctataaacg catctgcttc
     1861 tgagcaaaga gacatcgtaa aggccaataa cagcatgatg ataatggcta aaatctttgt
     1921 tgagccgagc gttagcataa ttctagttgg taaataagtc tgcatcgcag ctgcgattaa
     1981 tccgccaatg atcaaataac gacctgtatc aaaaaattcg tcgattgaat gtgtcaacac
     2041 atgcccagtt tgatggatca gctgattttt tggttcattc ttctcacttt gttcttcttc
     2101 atggacatga tgatggtgat tttctgctac tttagacgtg attttttctt tcaatacagc
     2161 gtctttttga aaataggcaa gccagacacc aatcacaagg gcaacaaaaa agctgcctat
     2221 cacccgccag cttgccatct gccaagaatt gccaaatgcg ataaaagtgg aaaaaatcac
     2281 gattggattg atgatcggag cagtcagcat aaaagcaaac gctgtgtgaa ctggtacgcc
     2341 ttttttaaca aattgatgaa cgatcggtac gatgccgcat tcacatgaag gaaagaaaaa
     2401 tcctaagatc gaaccagtaa aaatagagaa gatacgattt tcaggaagcc atctcttcac
     2461 acgttctggg gtcaaaaata cttgtaaggc gcctgagata agacagccta ataatacgaa
     2521 aggtaatgct tcaataacga tcgataagaa aatcgtactc atctgcaaaa ccgaatttgg
     2581 aataaaagaa aacatgagtt tcctcctttt tatttctagt tcattcgctt tgcaaatata
     2641 cgcttttatt caaaaaaaac aagttgctct gtaaattttt acacaactct tattttttac
     2701 caaagcactt caattgactt tttctgttat tcccattaaa ttaaggtctc atacatatca
     2761 cttctatcat aaaagggaga ataaaaatga aacaaaccac taacttgact gaagttcaag
     2821 acatactgca acagtcatcc gttgccgtca tttatctttc gatgcctaat tgcagtgttt
     2881 gtcacgctgt tcgtccacga ctagaggaac ttctcacaca ttatgatatt ccggcacttc
     2941 atttggatgc atttgaaaca ccagaagtag ctagtaaatt tgaagtgctg actgcgccag
     3001 ttgtcctgat ttttcatcaa ggaaaagaag tctttcggca agcacggttc atcgacttta
     3061 aaaaaatccg ttatctacta gatgaactga cagcagaaga tgattccctt tcttatgaag
     3121 aaatcttccg aacaaaataa tcgttgtcca aacagaaaaa agcgattgaa caagtgaaag
     3181 ttttgccttc atttgttcaa tcgcttttta tttttattct gctgtatttt cggaatgcgc
     3241 atttgcgtca gaatgcaaga aatagatcaa ggtttgcaat tcattcgtca aatcaacatt
     3301 ttggatcaat acatctgacg gagcattcaa tcgagcagcg gtaaagttca agattccttt
     3361 gattcctgct tccgttaact gattgactac ttcttgtgct ctttcagcgg gtatcgttaa
     3421 aattgctact tcgatttgtt ggatcttgat ttgttctgac atatcttcca ttggataaac
     3481 ggggataccg tctacgatgc gcccaacgat ttcctcattc acatcaaaag cacaactgat
     3541 acgtatgcta ttactttgat ggaatttgta ttttagtaaa gcacttccca agttccctac
     3601 accgatcagc gcaacatttg tcaattgatc gtcgtttaat gtctttgcaa aaaagtgcat
     3661 aagatcttct acatcataac catacccacg ttttcctaat tcgccaaaat aagaaaaatc
     3721 tcgacggatc gttgcactat ctacttgaac ggcttcactt aattcagttg aagacacttt
     3781 tttctttcca gcattattta aaatccttaa atagcgataa tacaaaggca gtcttttagc
     3841 tgtcgccttt ggaataatat gatctttcac gaaatgacct ccacgtattg tctaattaca
     3901 tctttgattt ctccatcata gcagtattta taggaaaaaa tcaattccat tgctcagata
     3961 ttgtgaaatt ttattcttta ttcatttatt tatcatttct gcaaatctcg cagtataatg
     4021 gaatttatgg tagactacta attgaaaaat agttttttat ttgaggagaa ttcacaaagt
     4081 agaaaagagg attccatgat attattacaa gccaatcaga ttgctcgcca ttttggtgca
     4141 gaagtcttat ttgaaaacat acatttagaa atcagtacag gcgctcgtgt cgctttagtt
     4201 ggcagaaatg gcgctggaaa atcgacttta ttaaagatca ttgctggaat tgaagcacct
     4261 gatgaaggaa agatcgctaa aaataaaaca gcgactctcg gatacctcgc tcaggacaca
     4321 ggacttgatt ccaatgaaac agtttggaac gagatgctga aagcttttga acaagttaga
     4381 caaatggaag agcggatgcg cgagctagag attttcatta gcgaaggtac gccagacaca
     4441 cctgcttata gttcttattt aaaagaatac gaccggctgc aacacgattt ttcagatcga
     4501 aacggctatg gttatgaaaa tgaaattcgt tccgtattgc atgggtttaa attcgattct
     4561 tcattttatg atcagaaaat cgatacttta tcaggcggac agcgcacacg tttagcttta
     4621 gctcgcatgc ttttgcaaca ccctgacatt ttgattctag acgaaccaac gaaccattta
     4681 gatatcgaga cactttcctg gcttgagaat tatctgcaaa gctatactgg tgcattattg
     4741 attgtttccc atgaccgtta ttttttagat aaagtagtca atgaagtcta tgaattaagc
     4801 agaagaaaaa tgcgccatta caaaggtaac tattcgaaat atctagattt gaaagcagaa
     4861 cagttagcaa gtgaatggaa ggcttatgaa aagcagcaaa cagaaatcca taaattagaa
     4921 gacttcgttg ctcggaacct tgttcgcgcc tctaccacaa aacgagcgca aagccgcaga
     4981 aagacattag aaaaaatgga ccggctggat cgtcctcaag gggatgaaaa acaagcaaac
     5041 tttctattca gtatagaaaa gccttctgga aatgtagtct tgcaaatcga agatgccgct
     5101 atcggatatg ataatgtcac cttatctgaa ccaatcaatc tagatatccg tcgtcaagaa
     5161 gcgattgctc tagtaggtcc gaatggtatc gggaaatcga cgttgctgaa atcaatcatt
     5221 caccagatcc cttttatccg tggaaaagaa acgctaggga caaatgtcac catcggctat
     5281 tacgatcaag gtcaagctga tctgcataca aacaagacga tcctagcaga attgtgggat
     5341 gagcatccta ccactccaga aaaagagatc cgaagtgctt taggtgcttt cttattttct
     5401 ggcgaagatg tagaaaaacc aatccctctg ctaagtggcg gtgaaaaagc acgggttgct
     5461 ttagccaagt tagcgatgaa caaagaaaat tttttgattt tggatgaacc aacaaaccat
     5521 ttagatattg acaacaaaga agtattagaa aatgcattga tcgattatga cggcacactg
     5581 ctttttgttt cccatgaccg atatttcatc aatcggatcg cgacaaaagt cgtcgaactc
     5641 tctgaaaatg gcagcaaagt ctatctagga gactatgatt attatttaga gaaaaagcag
     5701 gaagaagaag aaatcgccga attgttggca caagatgagc cagaatctgc tcctgctccc
     5761 acgaacaaat ctgattatta ccaaagcaaa gaacagcaaa agcttatccg tagccttcaa
     5821 cgaaaaatta ctcaggttga agaagaaatg gcgcggatcg atgaatggat cgatcagttg
     5881 gaacaagaaa tggtcgcgcc agaaaacctg gaagatcacg taaaactcaa tgagctgaac
     5941 caagatttag aagctgctag acaagatcaa gaaacaaaat tgacggaatg ggaagaacta
     6001 agcttggaat tagaagaata tgaaaattaa cgaaaaaacc aaaacagaaa ccattcatgc
     6061 tgttttggtt tttctttttt tattttatca aacaaaaatt ccatatttac ctttttcacc
     6121 aataagaaga ccaattacta gtcattctca ctaaagcttc taaataatag taatcacccc
     6181 aaatacaagg tgtatttacc ccattatttt tgggcttatt ataaacagac tgtgttaaaa
     6241 agccatcttc ttcttccgtt gtagcatatc tttctttcaa ttgtcgcata attttccaaa
     6301 cagcagactg gtataagttc ttttctgttt tgtctaatag acctgattct attaattcta
     6361 ataatccaca aaccatgatc accatactag atgtatcttt ttcttctctg ctagaatcat
     6421 caaagatcaa atcccaataa caaatggtat cttctggcaa ccgattcata aaataattgg
     6481 ctaatatttt gcttaaatca aaatattttt gttctttcaa ataacgataa cttaatgcaa
     6541 aaccgtaaat cccccatgct tggcctctag cccaacagga attatcacta taaccttgat
     6601 gcgtacttcc atatcttggt tttccatctt tgacatccat ataaaatgta tgataagttg
     6661 aaccatcttc tcttactaaa taatctgctg cattttgaat atgagcctga gcagcttttt
     6721 tatatttttg atttccagtt tcttcactcg cccaatacaa taatggaaga ttcatgcaac
     6781 aatccatgat cattctacct tgttgtgaag gatcactagg atctccccaa gcttgtataa
     6841 ctcctgcctt ctcccaaaaa cgctgaagta atgtttctgc agcatcaatt gaagcttcct
     6901 tcatttttcg atttcccgtt attttataat ctgctacggt tgataaagaa tacaaaaaac
     6961 caagatcgtg atgattagtg ataatttgac agtcaatcct gtggataaaa tctatatctg
     7021 ctttttcagc tgtttgttga aataaaggat cacccgtctt ctcaaataac aaccaattaa
     7081 ttcctgtcca aaatccttct gtccaaccaa tattgtctct taatggataa cgtcctttta
     7141 taggacactc gcttgggaat tgatactgac tgttactgat acgtttgatt aacctctccg
     7201 tgtgtctgcg acaacgctcg atctcattat tccattcttt ttccattaat tctttataac
     7261 attcatttat ccatttttct ggatgtatta tctcttcaaa catcacttta tcaagcattt
     7321 ccccaccagc ctttagcata attttagtct ttttttattg tttttatttt ggatagttag
     7381 cccggttgtc ccatcaaaat caattttggg acgattgttc catacttcta aactataatc
     7441 ccgatcttga tgagcaacca ctcctgttcc gagccaatgg gttccttttc caaatgtacc
     7501 ctctaatcca ggaataaaag tgttttccca tgtcatcaag tttgtattgg gcaaacccat
     7561 aatagctgtg gattttcgtt taatagtatc gtcacttaaa cagataatac ctgaaaagcc
     7621 agcttcactc accctatagt tttccttgtc ttcttctatg attattcctt ctttctcagg
     7681 agaacattca atagcaaacc ctgtctcata tagaacatat tctgtatcta attctacttt
     7741 gtgtacgcga atctgccagc cttcaaaagg aataagccaa gttctgattt ttgcctgtaa
     7801 agtaggttcc catgtagagg caagtacctc tttcttacat acaatgtctc gtacattatt
     7861 tcgaacgatg aactgtccat aggttttgat gctctcattc ttaaaactgg tcgtgatata
     7921 tgtgtccgct cgctgaatcc ctagcattga atccattgcc atttctgctt tcaattgatt
     7981 cgctcgcggt atcgaaaaac caaattgact agaataagca aattttaaat atttttcatt
     8041 ggtcaatgta tggaaatttg gccctaattg tcccgcattc aaaaaaaatg tatgtccatt
     8101 atcatgttgt atgagcaaat tggcttctga aagaagttta gttccttcac gaataggcat
     8161 aggcatttct ttagccgtcc aatactcatg gtcttcaggt agcgccaaca ataaaaaaat
     8221 cttattcagc cataatggag ataacgtact attatagggt tcagtcaatg tcaattgagg
     8281 gtaggtataa ccaatattca ggataccttt ttcatcaaat atttcttttt gcatccacca
     8341 tcttatattc cgattgacta accccttgat ttcacctaat ttaaaaggca aaagatcggc
     8401 ccacaacata gctgaccaaa agcttgaaac tgcaaaacga taaatcatgc ttcgaccaaa
     8461 aggcacattt gcaccatctt ccgcaaaaaa atgaatatag tctctagaaa aatcaatagc
     8521 tcggttctta tatatctggc taatgggctt ttctggttct agtacgctat aaatcaaacc
     8581 ataataatga aatgcaaaag gattataata atcttggcga cctcttgaac tgtctcgata
     8641 ccagccattt tgaagataac aattttctat attatcaaat gcttctttca gtgctgattc
     8701 tttaactgga taaccaaatt tttggataat tgttccgaca attatcttaa aaaattgcca
     8761 attaccatct gcacattcat attcaaaaat ttgcgagaac caatgaataa tctggctttt
     8821 ttctcctgta tctaattttt tccataataa ttgttcatga tgatataacg ctaataacat
     8881 tgcgggcatt tctaccatac gctggtctct ttcatgtatt tctccccaat aatcgggatg
     8941 ggaaggatct gttccagctt ttattcctga tagaattaat ttcaaccatc gttcatctaa
     9001 ttggtcaatt gcgggccccg ctccccataa aacccgagag aatccttcca ttcctgcaat
     9061 tttatttcca tatcctaccc cttgatgtat ataatcaatt tgtgcgccac ttgctcgata
     9121 aaacggctgg agtggttgat ttatcttttt aaataagtca aagtaatcct gtctggtcct
     9181 taatagtggt actggatgtt tcattgtatt ttctcctctt tttactacca aatagctaat
     9241 tccgactgtt ctaatcgaag taagccttca ataaaaaaat aatcagaata gatcagtggc
     9301 acattataag tcagttctct atggtatgct tctgctccgc ctccaatgat cccatcttca
     9361 tccatattcc aatttgcata gtgcttttct gtcttttgga gtatattgat agcagcttca
     9421 tgatacattt ttgatcgtac agaggaaaca tatcctgcta attccagaag accgcatgca
     9481 gcgcatactc cagcactggt atcaatattc gaatctttta cagcaaaatc aatgagagga
     9541 atataaccag ttttttcgat tttctgaata aaatttactg ctatcttttc tgctatagct
     9601 agatagtacg gattttttgt gtaacgataa cttaacgcga atccatataa agcccatgca
     9661 tttcctctgc tccaacaaga attctcattg tatccttgcc cgccaagtaa ccgaatatat
     9721 ttccctgatt ctgcatcaaa ttctgcgata tgcccggttg atccatcttt cctcactaga
     9781 tacttagctg ttgtatctgc atgctgccga gcaattttag agaacctcgg atcaccagtt
     9841 tccctcgaag cccaatacag taatggtaaa ttcatcatac tatcaataat catccaacca
     9901 ggtttatggt cattccatgc tactaaatag ttccctgaaa tattatagcg tccagcaagt
     9961 accgtagctg catgtaaccc tagttgtcga ctatgtttgt tttgagactg ttgataatgt
    10021 gcaatagccg atggtaagaa taaaaaacct acatcatgat gaacgtctga aaaatggtac
    10081 agggcagatt ctaataattg ttcttgttgt attgctgcct ctttgaactt ttctcgtttt
    10141 gtcttgtgaa agagctgcca taaaatacca ctccaaaaac cattggtcca ccagtcgatt
    10201 cctctgtcct ttattacgtc tgtatatttt ccgttgtaag aaatatatgg aattttattc
    10261 cccattctgt ctaattccgc atctatcttt tcagaagcta cattcaaaat ttttcttgac
    10321 cattccttcg tctgttcatt catggatatc ccgccattct tttaattttt atgaatgtgg
    10381 agttgctcct agaacgaact agatggttaa cattcgttat tttttttact tatttgcagc
    10441 tccaatgtca ttctccttaa atttagactg cgcccacaca taaatcaaga ccattgggat
    10501 cactgaagtt gctaaaatta gcataattgc gccataatta attggctcac ctgtactaga
    10561 aaccgtcata tttttgataa ttaagggaac cgtatattta tctttatctg aaagcatgac
    10621 taatggaaat agatattgat tccaacttcc cataaagata agtaaaccac tagtaataat
    10681 cgtaggtgat aaaatcggta cagcgatttt cgtaaaaatc acccactcat tggcaccatc
    10741 gattctggca gcctccatca gtgctgtagg aaattgactg ctatattgtc gcatcataaa
    10801 cactgaagtt gtactggcaa tcgaagggat gatcacacct gcataagtat tcgataatcc
    10861 taaacgactc ataatgtaaa ataaaggaat caatgtggta aaaaaaggca ccatcctaga
    10921 tagcataact gtattgaaca acagtttatt ccccataaaa cgatactttg ctaatgcata
    10981 acccgccatt gtagaactgg ctgtagctga aactgtactg attacagcaa taacaatcga
    11041 attccaaata atcaagggat aattatatct cgccatgatg gcttgccagt tttttaatag
    11101 catatcccca aacttgaaat tgattccgcc acttaaaatt tcttgattac tttgcgttga
    11161 agctacaatc atatataaat atggcatcaa aaatattaag gcaccaaaca caataatgac
    11221 gtacgtaaaa attctgcttt gtatcttttg tttagtcatt tttatcacct ccgaatttaa
    11281 attgaatagc cgataatata acagatacaa acaccatcgt ccaggcaata gcagaggctt
    11341 ttccaaaatc aaccatttca aaacttgttt tatataaatt gactcctacg accaaagctg
    11401 aattttctgg tccattggaa gtcccaaaga atatatttgg gatattgaaa ttacccaagc
    11461 cattgatagt ggctaaaact gtggccatta aaatgattgg ctttagcatg gggatcgtta
    11521 ataaaaagaa ttgccgaaat ttcgatgctc catctatttc agcactttcg tatatctctt
    11581 tagaaatccc ttgaagacca gctaaaaata aaatagtgtt atatcctaca ctaagccaaa
    11641 tcatcgtgat gatcattgat attcgtatcc aaaaaggatt tgttaaccac tgtacaccgt
    11701 taattcctag cgcattcaaa aatccattga ataacccagt cggattgaaa aaagtcatga
    11761 atacggaagc tacagccacc gttgatgtaa tggttggaat ataatagaca gttctaaaaa
    11821 atccttttcc ttttaactgt gctgaattca ataaaacagc aaagtaaata gctaatagca
    11881 tcgttattgg aattgatcct gccatgataa tcaaaacatt cgtgtaagat ttaataaaca
    11941 atggatctcg gaagatagct agataattgt caaatccaac aaattcaaat gagtagccaa
    12001 ttttcataaa actaaaagca aacgagataa tgatgggaat caaaatcgtc acaataaaga
    12061 aaataacaaa aggtgtgacc aatccataag cacttttttt atgagacgta tctgttgctt
    12121 ttttcttgct gttctttatt gtcttgcttg gtaacgaaga ttgcatcatc gttaagtcct
    12181 cctaatattt tttaatcacc agttaatttt acgccgtact tatgagcaaa attttctgct
    12241 tcttttagat atgaattttc aaaatcacct gtttgccaat agttatatgc agcggtagtc
    12301 aaataagacg tagcatctga ataagtggct ggtaaaacag tatttgatgt attttcattg
    12361 attttattca atagttcata gtatttttgt cctccttagt agtcaaactt tttctcagct
    12421 gcttctgttt gataggccaa tgagttagat gtagccacgc ccagctccag tgcgttggct
    12481 aagcattctt catctgtcag cataaatgta agaaattgtt gtgccacttg tggatttttg
    12541 gcatctttag ggacataaaa acttgatcct cctgatactg caccttttcc tggattttct
    12601 tttgaaaaag gaatcagtgg agccatgcgc catttgtcag atgcatcagg aaaattcaaa
    12661 gacatattcg tagcatacca gccgccagca acaaacattg cagattcttg aaatgctttc
    12721 tcagcatctt gtgcaccgta gaaatgtaca atatctgagt cgatcatttc tttgattaac
    12781 tttgcagcat gtttcgcttc tttagtattc agacggatat ttccttcttc atcaagcaaa
    12841 gattgattct gctgggctag catgttgata aacaattctt ggtccccaga ttctggcaag
    12901 gcaattccaa aactaccagt tttttctttt aatgcttttg ccacctggat atattcttcc
    12961 caagaactga tttcttcaaa atctacacct atttcagcaa atttttttgg ttgataataa
    13021 caaagtgctg gggtaaaatc actagcccaa ggaataatcg tttcattact cgtttgttga
    13081 cgcaaaatat tcaatttttg atcataaaac tgatcgccat atttgtcata aaatccatat
    13141 tcatcagcac tataaaaagc atcttgaaat ttttccagca cacctgctgc tgaagtgtct
    13201 gccatggaga taatatccgg catatcttcg tgtgttacca attttggggt gatcatttgt
    13261 actgttgctg ttcccgaagc tacattcaca atttttaagt caatatttgc tccagttttg
    13321 cggttataga tttttgcagc attttcaaca tagtttgtca gtttgccgtt tgcgacatat
    13381 acttctaatg tagtggcttt ttttatcatt ggatctgcga tgtttgctgt tacttcttcg
    13441 ttagtttttc cacatgcact taagctgaat aatagggtaa tgtttagcaa agctatcacc
    13501 aatttgtatc tttttttcat gtctatccct tctttctttt caagttctta tataaaacgc
    13561 tttcacttat gtatattaac ggaattagta acacaaatat ttatataaaa aaatatttta
    13621 cttgtcgttt tggcttttca tttttgttat aatagtcatt aattataaat agaaagcttt
    13681 ttatataaaa agagaaagga aagtggttct tttgttaaca aacaaccaat cctccaatct
    13741 tgtcaaaaaa acactaacct attccataga tacccatctt catttatctg ctgctcccta
    13801 ttttcgacta ctaataaatg aaagcgctaa tttatatgct gaatttgcac ataaatctat
    13861 agaactagat aatccttccc ttttattcat tgataaacag caagaagtcc aaatcttttc
    13921 tactcaaaag gaaacacaaa cttttatcat tatccagttt tattttgatg agcaattatt
    13981 agcacatatt acgggacaat ctttttctat agatcaagaa ataacatcta tcattacttt
    14041 tctgaaaaat gaacagttca aaattgatta tctaaaacat agtaagccag ctatttccta
    14101 tgatcaggaa atcaattctt ttttcatact atgccaacag atgagcattc atttgctttc
    14161 cttattagtt acttattgtc tgcaaaaaat tcaattgtct tccttaccaa ggcattatca
    14221 acaggaactt gctttattga caataccttc aaatgagaaa gtcattccta aaaaaaagca
    14281 tattctttat tcagatgaat tagttgatcc aatcatacga tatttagaag aacatctttc
    14341 agaaaatttt ttgcttgaac aattagccca aatttttttt atcagtccag ccagcttaaa
    14401 aaaacatttt agaaaagtga atggaaaaag tgttattagc tattttaaag aactaaaaat
    14461 ggaaaaagca aaacgtctcg tgctagaaaa tgaattaacc tatacagaaa ttgcagatat
    14521 attaggattt cattcgatcc atcatttttc ttcagctttt aaaaaatata cagggtt
//
LOCUS       DAJSKS010000064        14501 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJSKS010000064 DAJSKS010000000
VERSION     DAJSKS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.065"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..308)
                     /locus_tag="ON396_001628"
     CDS             complement(<1..308)
                     /locus_tag="ON396_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR3113716.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     A"
     gene            complement(308..538)
                     /locus_tag="ON396_001629"
     CDS             complement(308..538)
                     /locus_tag="ON396_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3113717.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            825..1163
                     /locus_tag="ON396_001630"
     CDS             825..1163
                     /locus_tag="ON396_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113718.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(1368..1607)
                     /locus_tag="ON396_001631"
     CDS             complement(1368..1607)
                     /locus_tag="ON396_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCR3113719.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1583..1930)
                     /locus_tag="ON396_001632"
     CDS             complement(1583..1930)
                     /locus_tag="ON396_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCR3113720.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(2662..3243)
                     /locus_tag="ON396_001633"
     CDS             complement(2662..3243)
                     /locus_tag="ON396_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3113721.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(3426..4052)
                     /locus_tag="ON396_001634"
     CDS             complement(3426..4052)
                     /locus_tag="ON396_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3113722.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4074..5405)
                     /locus_tag="ON396_001635"
     CDS             complement(4074..5405)
                     /locus_tag="ON396_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCR3113723.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5560..5961)
                     /locus_tag="ON396_001636"
     CDS             complement(5560..5961)
                     /locus_tag="ON396_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR3113724.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6047..6253)
                     /locus_tag="ON396_001637"
     CDS             complement(6047..6253)
                     /locus_tag="ON396_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113725.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6348..7850)
                     /locus_tag="ON396_001638"
     CDS             complement(6348..7850)
                     /locus_tag="ON396_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HCR3113726.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7868..8323)
                     /gene="argR"
                     /locus_tag="ON396_001639"
     CDS             complement(7868..8323)
                     /gene="argR"
                     /locus_tag="ON396_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCR3113727.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(8421..9038)
                     /locus_tag="ON396_001640"
     CDS             complement(8421..9038)
                     /locus_tag="ON396_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR3113728.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            9259..10632
                     /locus_tag="ON396_001641"
     CDS             9259..10632
                     /locus_tag="ON396_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3113729.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10707..11819
                     /locus_tag="ON396_001642"
     CDS             10707..11819
                     /locus_tag="ON396_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR3113730.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11891..13528)
                     /locus_tag="ON396_001643"
     CDS             complement(11891..13528)
                     /locus_tag="ON396_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR3113731.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13696..14304)
                     /locus_tag="ON396_001644"
     CDS             complement(13696..14304)
                     /locus_tag="ON396_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR3113732.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3953 a         3022 c         2556 g         4970 t
ORIGIN      
        1 gccatgtctt gcaatggtaa gttttctatt tttttgagtt ttcgttcctt aaaatcaaga
       61 gattttaatt gtgtaataat tacttgtcct tttatatcta gctcttcagg taatgagtaa
      121 cgtgtcggta aattctttat agtggaagta attggacata tgaccgaaaa cattgtttta
      181 cggttaaatt cataacgaga gacgactagt cctggtcgtc ttttttgaat ttcttttcct
      241 acggagggat caaaatcaat ccatacaata tcaccttttt taggaatata aattctttct
      301 tctgacacta taaaatttct ctcccttcag gagttaaatc ttcccattcg tctttctcat
      361 aatactcggc ctcttcccca ccactaaatg gatcttcaat ttttggaact aacgtaattg
      421 ttccatcatc agaatacaca acgatatatt cttggttttc tgaaggtttc tttccgttat
      481 cagaaggtaa agttaccact acagaacttc cttgtaatct agattttgta acaagcatta
      541 tcatcactcc cttaataaaa gtatactata taacactaag taacgcaaaa ttcccctttg
      601 taaactatct agaaaattgt tttgtttaaa gtatatctta ttggatataa atagaaataa
      661 attgtagcca gttcattttt tctttaacta aaaaaataat ccaagaaaca ttgaacctat
      721 ggatccaaaa gctacccaaa tactatagct aaccccccgc taccttcaat tttgcagata
      781 aaacgctttg ggagaaaatt tcaaagacaa caggccatcc tatcatgccc gattataaac
      841 aaaaagctgg ctattccttt tgctctgaca aatttcaatt gggaaagttc acgaaactag
      901 caggtgaaga agtttcaact gttcggatca aagaatgccc gcttcaaata gaaacattgg
      961 ttcaacgaat ttcaactaga caagactttg ctattgtaga acgtgagatc gtagctattt
     1021 ttgtagaaga agggatttta catgatccaa ctcatatcaa tgtggataaa tggcaacctc
     1081 ttatctataa attcagagaa tatacaactg ccaaccagtc gttaactttg aattttagat
     1141 tccaagaatt caaagagtca taatgataaa catcgttagg tataaataaa aaagactcgc
     1201 agcacgagta aaaaacaaat agaagctata cattagaaag gtcgactgtg accctataga
     1261 tgcagtatat gcagcttatg agcatttctt ctgttctctt tgtttttggg atctgtgctg
     1321 aagtcttttt cgttttttgc cgctctttct ttgaaaaatc gattatgtta taccttctcc
     1381 attactaatt caggcatctg ctgagtaatg atttttcgcg caaaagaaat atctgttacc
     1441 caaggttcga tttgttcttc agtcaagaca agcggcattc ggtcatggat tggtgcgact
     1501 gtgcggttag gttcagtagt cattatgatt gattctgcca ttttttcatc ctgatggcta
     1561 cgataaaaac cgccaatata cactattttt tccttatttg aaaacataaa tttgtctttt
     1621 tcttcattcc actcattggg aaaacacaac gattttgcaa aaatggttga tgaaaggtct
     1681 ttttttcttc tactgtctct gcacgggcat taatcatcaa ttgtcctttt ttaaatccgg
     1741 taaatcccca cttcatgatt tccggagtga ggatgttttc ttcctctttt cttaacgtca
     1801 caacaagatt tgacggaaaa acttctccag ctgctaattc tgctgcctgc ttcggttgtt
     1861 tcgtctgggc ttctcgataa taattctgaa ggagctctga ctctagatca aaaaaataac
     1921 gaccacacat cctaatccct cccaatcttt ttttctatca tacactgcaa caagaagttc
     1981 gcaccaacaa aacgctttca atttttgtag aactaaaaaa gttgtgacaa gtgacaaact
     2041 ctccagtgaa tggtattctc ctgccaactc ctcggtcaca agtcacaata ttcaacaaac
     2101 atgagaagct gctttttgaa attgttcctt gtgactcgga gtgaaacggc aggcttacga
     2161 cctctgggta aacggtgttc aaaagccagt ttctcggtat taagccaaat ttatgcaaaa
     2221 atgtagaaag ctattttcac aaaatttttc ttaatactcg aaactaagcg actttttcac
     2281 aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg
     2341 aaaaatatga gcagctattt ttatttggct gttcttatta cttgatgcag ggcagctttt
     2401 tcacaacctc tccactaaac ggtgttcaat cagctaacct tcggtattag ctcaaaaaac
     2461 ggcaaaatat gaggaactat tttgccgttt gtttctctaa tactcaggtc aaagcgctga
     2521 tttcacaacc tctaatacac ggtgttccag aagtagcttc ttcgaaaaat aagccgaaat
     2581 ttcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga agctgaccac
     2641 ttctgtcaca acctctaatt attaaagaac cttttgcgaa gttaacaatc tttctttgat
     2701 caattgaaaa ttctgcctaa gtagtgatag atcctgtgtg actttcaaca ataagatact
     2761 tccctggatt tcagcaatca acgctttggc ttgacggtct gcttcttcat cggacagttt
     2821 ttcttcagaa agtgccagga tattttgttt cgtatacttg acccattgat tgactaattc
     2881 tagtagcaac agacggtatt cttcatccac tgtactcaac tcaataatca aattacctac
     2941 aggacagcca tgataagtga tctgtgtctc atgatcagcg aaaatccagt ccaacatttc
     3001 ttctaatgct tcttctggct gttttccagc atttaagatg tcttcgaaca attctgtctg
     3061 ccatacgtcc atatgcgcat ggatcaccgc tagacaaact tctttttttg aagaaaagta
     3121 ataataaaat tgaccttttc ctgtatcaga agctgctaga atatctcgga ttgacgtttc
     3181 atgatagcct tttttataaa taagatcttt ggtcgcttcc aagatctttt cctttgtttt
     3241 caatgatatt ctcctttttc atttctcata gtcctatatt atacagtaaa ttcatcattt
     3301 tttcttctta atcgtttaaa aaagactgtg ccaaattatc tgacacagcc ctcactataa
     3361 aactgttctc atatagctag atgttcattg attcaagaac tgcgaggaca aaagcaattc
     3421 ctatcttatg atagttgctc taatacttcc tctgttgtca tcacttgatc tgcaataaag
     3481 ccaaagttcg tcaacgctgc atccattgct tcttggccag gagcaccaat tgcatctgtc
     3541 gctaccacaa cattgaatcc actctctacc aattcacgca tatgggaatc gctacacaag
     3601 tttccgttca ttccaccaag aattactgtg tcgattccat tttttctcaa ttggagcgcc
     3661 aaatcattgc tttctggtcc aaagatttta tgaggagagc tgatgatcgt attttcatcc
     3721 aaataaggtt tcaattcttc gataaaatcg gctcctgaac cttcttccac tgattcatat
     3781 tgactttttc tagcaaacat ctgctgctca agcatcatat gttctcctgc accgccaaat
     3841 tgccatttat ggtcatgtgg gtaaaaataa tgaggagaaa taaagagttt gtatcctttt
     3901 tcttttgctt gttttaccat ttcaatcata ttttcgatcg tattgtattt ttctaagata
     3961 tcttttgtca aaccaaagcc accgcctgct ggtttcaaga actcgacttg cgggtcagtg
     4021 agaactactg ctgtattttt attattgaac atggtggatt ctccattcgt tatttatttt
     4081 aatacatcat ttagagcttc actcattgtc ggatgagaat agatttgatc tctcaataac
     4141 gtataaggaa gttttccatt catagctagc gaaatcaagt tgatgacttc atgagaatct
     4201 tccgcataga tacttgcccc tagaatcaag tcagtttcag ggtcgactaa aactttgaat
     4261 acacctttag gatcttctaa tacttgtgct ttagggacac cgcttgtcat atacttgaat
     4321 aatttgtaat tgacgccttg tgctttggct tgtttttctg ttaaacctac attggataaa
     4381 gtcggagtga taaatacact gtaaggcaca gctttccggt tattcgttgt tcgtttattc
     4441 tcgcccttca actgatccat aatgattcga taatcatcta gagagatata agtgaactgc
     4501 aatccgcctt tgacgtctcc aatcgcccaa atgttgtctg catctgtacg aaggtattca
     4561 tccacagcga ttgctccgcg atctgttagt ttaacatctg tattttctaa tccaagtgat
     4621 gctgtacttg gttttcttcc agttgcagcc aaaacttttg acgcttggat tgttacttct
     4681 tggccatctt ctgtaaaggt gacggcagct gttgattctt ggtctgctac ttgatctaca
     4741 gatacaccta gatggaattc gattcccgca tttgtcatgt cttctaaaat catttgcgcg
     4801 atatcttcat cttcacgtgg aagaaattca gaatgggcat caagaacgac aactttagat
     4861 ccgtattcat tgaacattga tgcaaattcc aagccaatat atccagcccc gatgatcact
     4921 aatttttccg gtaattgttc ctgatccatc gcttgcgtag aatcaatcaa gtagcgactt
     4981 gtattcaatc ctttgattgg taaaatgatt ggttcagaac cagtattgat gaaaatccgt
     5041 tcgccttcaa caatcacttt ttcaccatta ttcgtcactt cgatttgatg gttagaaaga
     5101 aaacgtgctg taccgtccat cactgtccca gttgcctcat cgctgatcat atgataattt
     5161 ttggcgcgaa gcatgcccgt caatttttct ttccgagcaa cagcctctgt aaagtcgatt
     5221 cctctttctc cattgaaaat caatgattta gaaggaatac agccgatgtt gatacatgtt
     5281 ccgccataca tacgtgttga cttttcaacg actaaaaccg attcaccttt tgaagctaac
     5341 gccttcgcta aagtcttacc gccctttcca aaaccgatca caatattttg atatttttcc
     5401 atcatttttc cctcgcttct tttttaccta ccgattggta caaaaatatg ataaccgata
     5461 attgtctatt ctccaaatca tctgctttat ttgtcaaaaa aaaccagaca aaaagcggtc
     5521 ctttcacgtt tttagtctgg ttcgatttcc tttattcttc taaacaatcg ttacttctac
     5581 atcgatattt cctctagttg ctttggagta agggcagaat tcatgcgcat cttctgcaat
     5641 tttttgggct tcttcttcgc tgatgccatc gatatgcact tctaatacta caccgatctg
     5701 atagccgcca tcatctaaat tataaagaga gacacgtgct tttactgtgg actctgctgt
     5761 cattcctgct tctttgatca tatgttgtac cgccccattg aaacaagaag cataacctgc
     5821 tgcaaataat tgttctggat ttgttccttc tgcatggatt cctggaggag tgattttcat
     5881 atgcatcgag ccatttggtg cctcgacttc tccattgcgc ccgccatggt tgatcattgc
     5941 tgtttcgtat gcttttttca ttgtactgct cctttcgatt gaccatttgc tacttggcta
     6001 taattatagt gtatgacaag taaggcgctt tctacaaaaa ttctgcttat tcgaatacca
     6061 gaatgccgtc ttctactatt tttccaaaaa acaaaaaaat actactcgtg aacaacaacg
     6121 tcatatttgt tccaaaataa atagcaatct tttctacata atcttttata tgattgattt
     6181 taagatacat atataccaga aagttgaccc cataaaccaa tactgttaaa aaaataacca
     6241 tattcgttac cattttttcc tccttttgct ggtctcccta ccagcactcg atttaaccat
     6301 accacttttt ttgccgtcca agcaactagc ctttcaaaaa gactggttta ttctgctgtt
     6361 aatgattcaa gatcagaaaa atgtccgaat aattttgcta attttactaa cgccaacttg
     6421 ttctttccga attcatcatt ttctatctgt aatactagta acggctcatg taagcttaaa
     6481 cggatcaaaa accagccgtt tccgtatggc tcttctagat catatcgaat accttcttca
     6541 ttttccgggt cgaagttcat ccctttttct tcttccaaca gtcgcttcat ttcttctaat
     6601 atcttgtttc cttgttcgcg tggttcatcg ctgaacaatt ttaatcggat ttcttgtact
     6661 tctacgggct gtttcaactc tttgatttgt tcatctaatg tgataccttt tctttttaat
     6721 tctggcaaaa gcatcaggat ctttgcgatg acatatgcac catcatccag aaaatagttt
     6781 tccttgaatg cagcgtgtcc gcttgtttcg atggctagct gtgtatcttt cccaagacgg
     6841 ttaagttcta atgctttatt gattacattt cgatagccgg aaatataacg aacttggatg
     6901 cctccttttt cttcaatgaa tctttgtaga tgagaagaag taggggaatt cgtcacgatt
     6961 cttgttcctg gatgctcttt taatacgatt gtacttaata ctgcgatcaa attatttcga
     7021 ttgatcactg ttccagaagc actgacaact gcagaacgat ccacatctgt atcgaaaatg
     7081 atcccaagat ctgcttcatt tgctaaaaca gcctgcttga tactttccat cgcttcttta
     7141 ttatcaggat ttgggatatg attaggaaat cggccatctg gttccaaaaa ttgggaacct
     7201 gttgaatccg cacctaagct attcaatact ttttcagcaa agaatccacc agcaccattt
     7261 ccggcatcaa caatgatttt ccaaccattt agtggtttgc tttctagtgt tccaatcccc
     7321 tttttaattt ttgtgactaa atcttgcgca taaggtgtca aaaggtctgc tttttctacg
     7381 cttccagttt gttcgctttt tattttttgc tgctgtgata ggataaaagc aatatcttct
     7441 ttttctgctc ctccttctga ggtaaagaat tttattccat tataataata gggaagatgg
     7501 ctagcagtca gcataaccgc agcatcgcac gagaactgtt caaattgagt ggacataaac
     7561 atagcaggcg tagtagctaa ttcgaaatcg atgacatgta tcccttgttc tttcagactt
     7621 tggatcaacg cctctttcag tgctggtccg cttatcctac tgtcatgacc aatcccaatc
     7681 gtccattttt tatttggatc ggttgatttc ttctttttct ttatccagtt tgctagtccc
     7741 cagccgatct tttttaccat gtctgtcgtc aagtttactt catatccttc aactggtaat
     7801 gcgattcctc gaatatccga tccattttgc agcgctttca tatctttcat ataaaagtcc
     7861 tcctcattta ttccctcatt ttttccagct tttctgcaaa tcgctgtgct tcttcttctg
     7921 agcggcaaat aataatgaat gtatctacac cggccatcgt tccgacaact tcagggtaag
     7981 ccacttcatc tagccaattc gttaccacat cggcaccatt tcgttcagtc aatacgataa
     8041 ccatgaactg aacagactgg atacgcaaaa cctcgcgtct aattgctgta cgcaaacgtt
     8101 cttcatttaa agaaacagat ggctggttaa ataatgtata gcgtactatt ttgttttcat
     8161 tttgactttt tactaacttc atttctcgaa tgtctcgaga aatcgttgct tgagtcacat
     8221 cggcgcctgc ttcatgcagc cgcgcaagca gctcttcctg tgtcccaata ggttgttctg
     8281 taatgatctg gcggatcagt gcttgtcgct gtttcttttt catattagac acctctcaat
     8341 gttgattata gcaaaagata agtaatattt cttcatattt tggtattttt tacttttatt
     8401 ttttgttttc attcaggttg ctaacctttt tctattttaa cacgcaaaat cgtcttcatt
     8461 ttttcaggtg ctgattttct tggatcgctg atgtaaatct ctttgtgttc tttagaagct
     8521 ctttcatagc cttcttttgc gatgaatgct tctaatttct caaacgttgc tggttcttcg
     8581 gcatagggcc cgacgtgtaa gatttgggca caaagacctt cttccatctt tttgaatact
     8641 acttgttctt tcaagtcttc cggaagtttg ctagaacgct gaatggcttc ttgtgctatc
     8701 gcttctgtaa caaagtcagg ctgtcggatc atcaattgat aagaaaaatg ttcttttttc
     8761 atcactggtt catgcaaata cttttcttgc agtccccatt gtccttccag tggatagaca
     8821 gtatacatct gataatcagg aatcagccaa ttttttttag gcgacatgcg tattgcatag
     8881 ctgactgcat atagacaggc tactcttcgt ttaaagtctt cctgattcgg gtcgccgatc
     8941 cctctgatgc taaagaatga ttttttagga acctctagta tttgcgggct ttttttcata
     9001 ctatagctct tgtctttacg ccattcaatt ttttccattt ttcttcctcc gcgaaattcc
     9061 atttagttat ttattatcat acctcttttt gagtgacttg gctaatcgtg ttgacactat
     9121 ttttcagttt gtgctagaat aagtgaagta aataaatcgt acagataaca cgtttcggcg
     9181 tgtcgagaga gaggcggttt ctatttttat aatagaaatc actctctttt tttacgaaat
     9241 tttttagagg tgatgtaaat gacaaaacaa aaacagctgc ttttagtacc tgcagtaatt
     9301 gcaacaggga tgatgtcttt tgcgggcgta ttgatcgaga cggccatgaa tgtaactttc
     9361 ccaactctta tcagacagtt tgatttaacg actgcacaag tccaatgggt aacaacgatt
     9421 tatcttttga tgatttcgat cattgttccg ctgtctactt atttgaatcg caattttagt
     9481 ctcaaaaaac tctttttagc ttcaaatctg cttttcttgg caggtataat gatcgatttc
     9541 ttctctccta ccttcatggt cctattgttt ggtcgattgc tccaaggtgc agcgacaggt
     9601 atcgctcttc cgttgatgtt ccatattatc ttgacatttg cccccctcaa tcgacgaggc
     9661 gcgatgatgg gtgtcggtac tttgacaacg gccattgctc cagcaattgg ccccacttat
     9721 ggaggcatta tgacttcaca ttttacatgg aatcatatct tcctgtttct tgtgcccgtt
     9781 ttgtttcttt ctttgattat cggctggttt gctattcccg agatggcagt acaaaaaagt
     9841 ggttatttgg attggataag cgttttagga atttgtcttt tgtttagtgg aagtctaaca
     9901 tttttgagta cgttgggaac agtttccagc tggattgctt taatcatcgg cggtattggc
     9961 tggttccttt tttactatcg aagtaaaaaa gtggagcatc cactgatccg actgtctatt
    10021 ctttcaaata aaacattccg cttgtttttg attggcttct tagtttatca gtttttatta
    10081 cttggtgtat cttttgtctt gccgaatttc cttcagatcg ttcaaggaag tagtcctttt
    10141 gtcgctggat tagcgatgct tcccggcgca gcggttggtg ctttgctttc tcctttttcc
    10201 gggaaaatgc tggatcaata tggtccgaag aagccaattt ttgctgggct gattctttct
    10261 ttgattggtt ggctagcatt attcctactc ataggacacg cttcagtggg tgtattgatt
    10321 gcttgtcatg tgttttatat gatcggtatc ggattatctt acagcaatat gatgaccacc
    10381 ggaatgaatg cactgaatga agaactacaa ggagacggga acgcaatgtt caataccttg
    10441 cagcagtttt ctggcgctgt ggctacttca ttagtagcgg tcattatcaa ttacgtccag
    10501 catcatactt cccataacta tgaagtgtct actacattag gatcaaaagc agcattaggc
    10561 gtcttgttgc tgttgcttct cgtaagcttt gctcgattcg cctattatct gttctttgct
    10621 aaaaaagcgt agattccata tacggcaggt gttgcatctg ccgttttttt gtgtgaaaat
    10681 ggttgtttgt aaaaggaggc ttttttatgg aaaaaattgc cattatcggt ggcgggatca
    10741 ttggtatgac cctagccagt tacttagata catcaaaatt caatgttgct ttatttgatg
    10801 aagggacagg acaggcaact aaagctagcg ctgggattat ctccccttgg ttgtcgaaac
    10861 gcagaaacaa acaatggtat cgtctggcta aagacggcgc tgcttttttc cctaaactta
    10921 taaaagattt ccaacttgat caaacgattt acgcacaatc agggacactt ttgcttcgac
    10981 ctgaaaaaga tttagctgaa ttggctgttc ttgccgagaa acgaaaagaa gaagcgccag
    11041 aaattggaaa catcgaatgg ctttcacctg aacagacatc tcaagcactt ccgttattga
    11101 aaccagcagt ttcactgcgt atcagtggtg gtggtcgtct ggatggaaaa gcatatttag
    11161 aacatcttgg tcagcgttta gcacaaaaac aaatggagca gatccaggca aaagtcactt
    11221 tgcataagaa gagcgattct tggctcatca agggaccgtt tggagaatac acagctgatc
    11281 gtgtaatttt gacacctggc ccggcactta agcctttatt acaagaatta ggctatgctg
    11341 cagatatacg tccgcaaaaa gggcagctct tagcatttca gacatcttat aagatgagtc
    11401 aagactggcc tgttgctatg cttgacggag aagctgattt cattcctttc atggatggaa
    11461 agatcctttt aggtgctacc catgaaaacg atcaaggctg ggatttggct cctactagcg
    11521 aagcttataa gggattgact aagagtacag aacagttctt gactgatcca gatcagataa
    11581 aaaactggcc atacgagttc cgtgtaggta cccgtgctta tacgtcagat ttttccccat
    11641 ttttcggcgt actgccagat gatccaagct tactggctgc tagcggtcta ggctcttctg
    11701 gcttgaccac aggtccttat atcggctatc tattagccaa cttcttaaat actggtcatt
    11761 ggcctacgga agattatcaa aaggacatct ccacctatat tacgaaaaat ccatcataga
    11821 aaaacagctt tttcaagtaa ggatcccgag cgatattgcg catggattac ttcttgaaaa
    11881 agctgttatt ttagttcaat ttctgaatca aattctccaa actttctttt gcttcatctg
    11941 gcaagagatc ttttcctatc tgagcttcaa tgaatcgtaa acgaatatca agtcgttgtt
    12001 tcaccagatt tttataggac gtatcctcaa aagaaacagg aaaatctggt ataggtaaat
    12061 agtggatgtc ttcaaatgga ccaaacgact tgaattctgc cgtttgttgc acgattcttt
    12121 caacgattga caatgtcgtt tcaggtttga tttttttatc atcgaaatat ccagtattca
    12181 ggacatagca ggctgtttgc tgtttcaaaa acaactctcg aaaagcttga tagtcttccc
    12241 taagaggata gacacggaaa ggattagcaa atggttcaat gactagctca tctagctgtc
    12301 tttttgtagt gatattttcc gcgtctgatc gttttgttgc taatgttgca ccaaaagacg
    12361 ctgctaaaac tgggtcattg atcttgataa ttggcggaag cgaatcatct ttcatgatcc
    12421 aaaaaacagc atcgatcttt tcgctaagat gatcgactcg gtcgggtgtc gcataacgtg
    12481 atttgactgt ccgcccattt ccgtttcgta tatctcctgt tactaggact ttttctcctt
    12541 tacttgtaca tgtcacgccc acattctgac aagtaaggaa agaagaaatg gccggatcat
    12601 cagacggata gtcttgtgtt ttatcaaagt aagaaggttc caaggcagta gcaaaaccat
    12661 tttttcgaga aataacgaaa gcatcgtcat gcaagatcga aacagagtga ccttccgtat
    12721 gttttgctaa ggtaatcgtt gattttccag aaccggacaa cccaaataca gccatcgtat
    12781 aggtctggtc attactagca tattgtttca tcccaccgtg gcaggcaata aagccattgc
    12841 gatttcccaa tgcccaagca agcgtcaaag tcgctttttt taattctcca aagtaacgta
    12901 atcctagcac agccgctgta ttatggagag catcaaacaa aatgagtcca tttggaaatg
    12961 tttcatgtgt ccattcggga tcacaaaaga tataaagatc tccctcatca aaggaaacag
    13021 acttttgata agtctctaag atcttcgaat tcgctggctg aaaattcagc aaataagaat
    13081 acaaattgtt ctcaaatcct tctggaatca gcaaatgact ttgcacagaa aattcttcag
    13141 ataggccaac gattactttt gtatggtaaa actggcgctt gcgactttga tacacagctt
    13201 ctcttaaaat tttctcgtat ttgctttgtt ccttgtctaa gtcctcaaag atccgccgag
    13261 cacttgctgt tcttcccaca atgcttccat cattgaatac tagtgtccta acatcagctg
    13321 gtaaatctag ttcttcagca tgtgagattg ttaaatctgt gatgatcgtt cctggactcc
    13381 cttttgctag ttgataagct gtcttcctat caaaaacttc atgaacatta ttaccgtaaa
    13441 atgtcgtttc tacaatcgat cgaatactgg ataacagtgg atttccactt gtgatttcgt
    13501 ttaattcgag actctctttt gtactcatgg ctcctcctca ttccctaaac aaaaaaacgt
    13561 tttctttatt atacattatt cgagttttta tttgtactgc tgcgctgtgg gaagcaaacg
    13621 agttattttt cacacaaaaa aaccgagaac ttgaataaac aagtttctcg gtttcatcat
    13681 tttcctttta tcggcttagt accagccgtt tgctaaccag aatgttttag cagcatccca
    13741 tgaaccataa cgagatgtta catattgttc tgctacacgt tcttgattgg cagctgaata
    13801 gtcgccgttt aagtatgaag catctaattg ataacggccg atgtaacgtc cgttagtagc
    13861 tgtgtatgaa ccgcctgatt ctttttgtgc gatccattct tttgcagagc ttgaagttgg
    13921 cgctgttgca gcagctgggg cagcaggagc tacttcaact gtttcaacag gtgcaacgta
    13981 tgtatgttct tgcgttgctg tttcagttgt agatgctgta ttttctgttg ttttgttttc
    14041 tgttgttgct gaagaatcat ttgaagttgg gattgttaat tgttcaccaa cataaatgcg
    14101 gttgatgttt gcgatcgaat ttttttcagc aattgcatcg atcattgagt tgtctcctgc
    14161 aaatttttgt gagatcttag ataatgaatc acctgatttt actgtataaa cttcatcagc
    14221 gtgagcattt gtagttccta caaacattgc agtagcacca gcagcaaaag ttgtacctaa
    14281 aacaattttc ttaaatgaat tcatattaaa ctcctttttt tctccgtttt ttattctttc
    14341 gacgctatta actttatcat ttagaaatat ttcataggta aaagaaacat gacatttaat
    14401 gacaaaataa cgaattattt aatatttgta acatttaaac aaatattttt ttgatacaaa
    14461 accataaaag ccttgttgta taagcaaagt aatcgttttt c
//
LOCUS       DAJSKS010000065        14428 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJSKS010000065 DAJSKS010000000
VERSION     DAJSKS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.066"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(59..2134)
                     /locus_tag="ON396_001645"
     CDS             complement(59..2134)
                     /locus_tag="ON396_001645"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR3113733.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2136..3053)
                     /gene="glyQ"
                     /locus_tag="ON396_001646"
     CDS             complement(2136..3053)
                     /gene="glyQ"
                     /locus_tag="ON396_001646"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR3113734.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3439..4257)
                     /gene="recO"
                     /locus_tag="ON396_001647"
     CDS             complement(3439..4257)
                     /gene="recO"
                     /locus_tag="ON396_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR3113735.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(4400..5299)
                     /gene="era"
                     /locus_tag="ON396_001648"
     CDS             complement(4400..5299)
                     /gene="era"
                     /locus_tag="ON396_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR3113736.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5314..5709)
                     /locus_tag="ON396_001649"
     CDS             complement(5314..5709)
                     /locus_tag="ON396_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HCR3113737.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(5693..6169)
                     /gene="ybeY"
                     /locus_tag="ON396_001650"
     CDS             complement(5693..6169)
                     /gene="ybeY"
                     /locus_tag="ON396_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR3113738.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6198..8390)
                     /locus_tag="ON396_001651"
     CDS             complement(6198..8390)
                     /locus_tag="ON396_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HCR3113739.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8414..9385)
                     /locus_tag="ON396_001652"
     CDS             complement(8414..9385)
                     /locus_tag="ON396_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR3113740.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10090..11469
                     /gene="brnQ"
                     /locus_tag="ON396_001653"
     CDS             10090..11469
                     /gene="brnQ"
                     /locus_tag="ON396_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCR3113741.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            11751..13091
                     /locus_tag="ON396_001654"
     CDS             11751..13091
                     /locus_tag="ON396_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR3113742.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTILRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPLSMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKRDRRS
                     KHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVDRLLSYDDSLKRGYEVYQDF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            13248..>14428
                     /locus_tag="ON396_001655"
     CDS             13248..>14428
                     /locus_tag="ON396_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR3113743.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     L"
BASE COUNT         4016 a         3073 c         2680 g         4659 t
ORIGIN      
        1 caaaaaaaac agagacacag cacgtttcgt gcatgctctc tgtttttcaa acaaaagact
       61 attttgtgat gatttgatcc aagcttgcta atgacaaagc catggcagct atttgactta
      121 gttgtgttag tcggttctta cgtactgctg gttcatcagc catgaccatc gtttgatcaa
      181 aataattttc gatcagtgga cgaagagcga caagctggtc gtagttttcg tggatcgttt
      241 ggtcagcaaa acttgctttc attgcttcta ctgctttgtg taattctttc tctgcgtcgt
      301 tttcgaacag ctcagggtct acttctgatt gtttttcaag ctgcgctttt ttcgctaagt
      361 tcacgacacg tgtcaaagct tccattgaag gtttgaagtc ttcatctgca agatgttgtt
      421 tgaacaattg tgctgctgca aatacttttg tcagatcttt ttgttccgca ttcaaaacag
      481 catcgatgac atcatgacga atattcttcg ttgtcaataa ttggcgcaga cggcctttga
      541 taaagtcgat cacttgttgt tgcccttctg ataaaccaat gccgaaacga tccgtatctt
      601 tactgataac atcgctaatc gtttcttgca gaacagacaa aggaaaggcc cattttttcg
      661 attcgatgat ccgaacgaca ccgtatgctt ggcgacgtaa ggcataagga tcgtttgacc
      721 cgcttggaat catcccaact gcaaagaaag aaaagatgct atctaattta tctgcaatcg
      781 ctaaaacaga acctactgta gactgaggta gctctccttc actggatata ggcatgtaat
      841 gctcacgaat cgctgtcgct acagcaggtt tctcgccttt cagaagagca tatttttcgc
      901 ccatgatccc ttgtagttca ggaaattccc ctaccatgtt ggttactaga tcaaatttat
      961 agatttcagc agctcgttgc aaatccatca attcttcctc agatagtcct acttgttcgc
     1021 caatgatacg ggcaatgact tgtacccgtt gcattttctc atagatacta ccgatttttt
     1081 catggaaagt aacagttttt agtttttcaa cgtattggtc gattgaagct ttcttgtctt
     1141 cattataaaa gaattccgca tcttctaaac gagccgtcaa tactttttga ttgcctcgaa
     1201 ccacatcttc caattttacc cgatcaccat tacgagcaga aataaaatga ggaagcagca
     1261 taccttgatc attccgtacc tcgaaataac gttgatgttc tttcatagaa gtgactagta
     1321 cttcttcagg aacagaaaga tatttttctt cgaagtcgcc agaaaagaca gttggatatt
     1381 cgactaaatt gttgacttct tctagtaaat cttcgtctag atcaataacc cagtgatttt
     1441 tttctgctaa ttcgtttgct tgtgtaacga tcatttcttg tcttttttgc ggagacgcaa
     1501 tgacgaattg ttcattcaat ttttcttcat attcttttgc atgcgcaaat gtcacatctt
     1561 cgcctaagaa acgatgacct cgagaggctc tgcctgtttt gacatccaag atttcgaatg
     1621 ggattacttc gtcatctaac aatgccacga tccagtgaat agggcggata tattcaaaat
     1681 cgtattctgc ccaatgcatc gttacaggga aagtcaaact tttgatcacc cctgctaagc
     1741 ctgtcaatac ttcttttgct tccttaccat gagtgtattt cgttacatag acatattcca
     1801 caccttttaa ttctctaaaa gtgatagcgt ctgtcgtcag accttgtccg cggacaaatc
     1861 cttccgctgc tttcgtccag tttccatctg cgtccaaagc aatttttttg gctggtcctt
     1921 tgacttcttc ttcactgtcc gcttgtcgtt ctggaatatt tgttactttg attgctaaac
     1981 ggcgcggtgt ggaaaatgtt tcgatcgtat cataagtcaa ttggttttca tctaaaaatt
     2041 ttgctgtttt ttcttctaat tgtttcatgc ttggtgtgac aacatgggca ggcatttctt
     2101 ccagcccgat ttcaagtaat aagtcttttg ccatcttatt cagcctcctt cacggtttct
     2161 ggatttgctg tgtttttatt caacaaaggg aatcctaatt tttcgcgttc tgccacaaag
     2221 attttggcta cagcacgagc catgttgcgg atgcgagaaa gataaccggc acgctctgtt
     2281 acggatactg caccgcgggc gtctaataag ttgaatgtat ggctacattt caagatataa
     2341 tcatacgccg gatgaacaag attttcatcg atacagcgtt tagcttcttt ttcaaaacga
     2401 tcaaagttgc ttagtagcaa gtcttggtcg cttacttcaa aagaatattt ggaatgttca
     2461 tattctggtt gtttgaagat ctcgccatat ttcacgccat cagcccattc taaatcatag
     2521 acactttcaa cttcttggat ataagaagca agacgttcta atccataagt gatctcagaa
     2581 gttactggtt tacaaggcag tccaccaact tgctggaagt acgtgaattg tgtgatttcc
     2641 attccgtcaa gccatacttc ccaacccaca ccggcacagc ccatggatgg gttctcccag
     2701 ttgtcttcaa caaaacggat gtcgtgttct aatggatcga ttcctaaaag gcgcaagctt
     2761 tctaaataaa gctcttgaat attctcaggt gaaggtttca tcaccacttg gaattgatga
     2821 tgctgataca aacggtttgg gttttcacca taacgtccat cagctggtcg gcgagatggc
     2881 tctacgtaag cagcattcca tggttctggt ccgattgccc gtaaaaaggt atatgggctc
     2941 atcgtacctg ctcctttttc tgtgtcataa gcttgcatca gcatgcagcc gtttgatgac
     3001 caaaattttt gtaatgttaa aatcatatct tggacggtca attttttact catctgtttt
     3061 gcccctttca attttaaagc gttgctgtca aataagaata caggaaagaa aacgacaaaa
     3121 aatccctatg tctgattaca gacataggga cgaataattc gcggttccac cctacttccg
     3181 aataaagatt cggcagcttc atttatcgta ctcgcaagtg cctcttcaaa aatcgtttgg
     3241 gtccggctcc cactctcccg aactcgctaa accacgtgtt ttttactgat cttggtcaat
     3301 gtacgtattc attttgcagt tttcttgttt ctataataga tcctatcact gaacgatcta
     3361 tttaatctat ggttaataat agacggaact ccctgtgttt gtcaaatggt tttgattttt
     3421 tgttgtatgt tttatttact attcttcttt ttcaggtgtc tctttttctt tttcagacat
     3481 gtctttctct gacgtcttta atacgtgtgc ccaagacttc atttgattga tgaacttttt
     3541 gcttttcaaa tggataccga catattcatc atacagctgg tcgatgagtt cacggattgc
     3601 ttgtttggtt tcttctttca gatggattcc actaatttta tcataagaaa tagctgaaaa
     3661 catccgaacg aaataaacag ctcgaggatc tgcatgataa cggtgatcgt ccatcgacca
     3721 atgtctttcg catatcacac caccatattt ggaagaatag tcaaattttc cttttgtttc
     3781 cccgcatacg acgcagtgtg tccagattgg tgcaattccg aagcgttgca gtatttggac
     3841 ttcaaaaata ttcgtgatga tttctgcatc gtctccttgg tccatccgtt gcagtgcttg
     3901 ttgcgtaaac tggaacaaat gcgggtcgta ctgatgatct tcgattgccg catctactag
     3961 attcaataaa tatgtgccat agccgctcaa aaagatgtct tcttggatgt gtcgaaacgc
     4021 atggacatct ttgacactgc ttaaaaatga caaaccttca cttcgaaaat tcccgatata
     4081 aactgcttca gtaaaaggga gcaaagccgg agctaagggc tgattttgcc gatggatctc
     4141 tttgatataa aacatcattt tgccagaaga ttctgtaaag attttgacta atttatcttt
     4201 ttctttgtaa ttacggctag ataaaatcaa gccttttgat tctgcaagct ggctcatatt
     4261 ctccctccta aaaaataaat ctggaaaagc agattctgcc gatccagatc atttatgttg
     4321 cgatcttccg cagcttttct caacacgcgg atttttattc agtttatttc tatacctaat
     4381 ttacaagaag ctcagaatat cagtattcgt cttttcggta gccgaagtct tgcagataga
     4441 cttttttgtc tcgccagtct ttttgaactt tgacccaaag ttctagaaag actttgtctc
     4501 ctaaaagatt ttcgatatct tttcttgctt tcgttcctac ttgcttcagc atttttccgc
     4561 ctttgccgat aatgatccct ttttggctat ctctttcgac aataatcgta gcctggatat
     4621 ggattttatc attctcgttt cgcttcatcg aatcaacaac gactgcgaca gaatgaggca
     4681 cttcgtctcg tgttaaaaac aagactttct cacggatcaa ttcagaaacg atgaagtatt
     4741 caggatgatc agtcacttga tcatctggaa aatattgtgg gccctctggc atttcactga
     4801 ctaatacatc catcaaacgt tcgacgttgt ttccttctgt agcagagatt ggaatcactt
     4861 gtgcaaaagg catctgcgtt gaataatctt cgatgattcc caacaactcg tctggatgga
     4921 ttttgtcgat tttattgata atcagataaa caggagaatg cacgtttttt agacgttcaa
     4981 taataaaatc gtctccccga ccgcgttttt gatcagcact aatcataaat aagaccgcgt
     5041 ccacttcttt caacgcactg taagctgctt ctaccataaa atctcctaaa cgatgctttg
     5101 gtttatggat tcctggtgta tcaataaaaa tcaactgagc ttctggtgta gtgtatactc
     5161 cttggatttt atttctagtt gtctgtgcct tgtcactcat gatggcaatt ttttgaccca
     5221 cgattcgatt caataatgtg gatttcccta catttgggcg gccgacgatt gcgacaaacc
     5281 ctgatttatg ttctggcata tatgttctcc tttttaatta aacaacaaat gatatatttt
     5341 agggacaaat aaaatcaacc caacgatgac ggtaaaacaa gcggtcacta aaactgctcc
     5401 agctgccata tctttgattt ttttcccaat cggatgaaaa tgaaaatccg taaacatgtc
     5461 cacgacattt tcaaaaacag tattgatgat ctctactatc catacgagaa aaacagctaa
     5521 aagcagccat aaccattcta tccgatcaag ttggaaaatc acgcccataa acaaagcgag
     5581 gattccgaac gcaacatgtt tcctcatgtt gcgttcttcc tcaaaaacag ttttgactcc
     5641 ttggacggca aattccacgg acatcaggaa atgtttgttt ttctctacgt ttttatcttt
     5701 taagtccata ggcatctaag atctctttct gtaaaccaaa catttctttc tcatcctctg
     5761 gtgtcatatg gtcatacccg ttgatatgca agaatccatg aaccgctaaa aagccaagtt
     5821 cccgatcata cgaatggccg taagcttctg cttgctctgc agcacgttca gtagagatca
     5881 tgatgtctcc cagtacacga ggcaatggat tctctccatc ttcaaaaatc actgggattt
     5941 cgtcttcgcc ctcttcttcc aaggcaaaac tgatgacgtc tgtcggtgca tcttttccac
     6001 ggtaatcacg attgattact tggattgctg cattgtccat gaaagtaaca gacatttctg
     6061 tttcttctgg caactctaaa tatgatgctg caaattgcag caagtcgtcg atttctttca
     6121 ttttctcttc tgagacttgg tttgtttcat ccatgaaggt aatatccatt atacatcgct
     6181 tctttctatt cttcttgcta ttcttcttgt tcttctttta tcttctcaac ttccgatttc
     6241 attttaggat atttgatccg agaatggaag gtactgctta aaccgctgat caatgatttt
     6301 tctactttac ggatttcttt taacgtcaat ccactgtcgt ccaattgtcc atcggaaata
     6361 cgctcttcaa taagattatg gacaaaactt tcgatttttt cgctcgttgg atggtccatt
     6421 gcccgtactg ccgcttcaca actgtcagct atattgacga ttcctgcttc acggctttga
     6481 ggttttggtc caggataacg gaattgctct tccgtaactt ccgggttgcg ttcctttgcc
     6541 ttaacgtaga aataacgcat cagcgtggtt ccatgatgct gtctacaaat atcgatcacc
     6601 atttgcggca tattataatc ttccaatatt ttggcaccat ctgtcacatg tccaaagata
     6661 atatgtttac tgtcttctgg caataagaaa ttgtgcggat tttccgctcc tgaaggcaag
     6721 ttctcaacga aaaagctggc gtgtttgatc ttgccgatat catgataata gcaagctact
     6781 cgagttagta aagaacgtcc accgatttct gctacagcat tagcacttag attggctacc
     6841 atcatgctat ggtgatacgt acctggtgct tcttccagca actgtttcaa taaaggatga
     6901 ttgggattgc tgagttcatt taatacgatc acactatcat cattgaccat caattcaata
     6961 taggggtgca gacctatcgt cagcaagaat gaaaacagtg cacctgtgaa tccgcagact
     7021 agcgttaacc aagtcgttgc atccccaaag ctcattcctt ggtagataat caaaatgaca
     7081 tccatcatca caggaaaaat aatgacccac atagcagcgg ataacccttg ttctgagatc
     7141 cgtttgcgtc tgacgatcgt tgccatgaga ccagaaaata cataggacat caaaataact
     7201 gtcagaaagt tcgtgccaat cgaatcataa aaaatgaaaa gtgcggatac tgcttgaaaa
     7261 ataccagcca tgatccctgc tcttcgattc aagaaaaagc ttaaaacaag tggagcaaaa
     7321 gccgcggggt aaaacagcgg aatataagca gcttgttctg tttggaagag ttggaaaaat
     7381 ttcattagta cgacgcttac tgacattgcc gtcacgtaga aaagtacgta ttttgttctt
     7441 tctcctgcat tttggaattg aagtgaatta taaatcagta caccgatttg aagcaaaaca
     7501 gctaaaacca tggcaatgag tgggaaaata gaagtcgttt gattcgtcaa tcctagtaat
     7561 tccaattttt tcatcgctga agcatcaatt tgatttcctt cgcgaacaat aatttctcct
     7621 tggaagatca tcactggttg tacagcttct cgtgcagatt gttttaattc ttctgtcttt
     7681 ttctcattca atacatcatt aactacgatg ccttgatcca ctaagtagcg aatcatttgt
     7741 tgctgctctg ttgtgacatt caaatattga atctgatctt ctgcttcttg tttaaaagct
     7801 tccaattcgc tttcacgaac ttgtttactc atctgttcgt caatgagctt caaactttca
     7861 tccctaactg tgtgaatttc cgtttcagtc atttcaaata tcgtcgaata aaaattgttc
     7921 ggcagttttt ggtaaaacgc aacgttttct gcattcacgt tttcaaaatt tttcttcagt
     7981 gtagcaatcc gctcttctgt agtaggtttc gggatatttt catcttcctt cgccttatct
     8041 accttgccat tataagattt gttgatggta tcattcgtct tatcgatcaa ttcgaataat
     8101 tgcttgatcc gattgttctg gtcatctgcc agatcttttt gataagtata ttctggagtc
     8161 actgcttctg ctgccagttt tcttctttgt tcggtttctt ccgtgttctc aatcgtttta
     8221 ttcgcacgga tactctcttc tgctacttgg ccttctctga aatcaatggc cttttgtttg
     8281 acactactaa atgtaacgcc gcagacaatg ataaaaaaca agaacagaag gaaaggcaat
     8341 gctgcctttc cgagtttttc ttgtagtttc tgcagtgttt tattcaacat gtgcttatcc
     8401 tcctaagcaa agcttattct tgatgaagat ccgccttttc ataagctttg atgatttcgg
     8461 caactacagg gtgccgaaca acgtcacttg cttcaaaatt aacaaaatcg atttttttga
     8521 tttgctttaa tgttcgttct gcatgaacaa gaccacttgt cgtcccacga ggcaagtcta
     8581 tctgacttgt atccccattg acaatcattt ttgattgata accgagacgg gtcaagaaca
     8641 ttttcatctg agcgactgtc gtgttttgtg cttcgtccaa gataacgaat gcatcatcca
     8701 atgtccgccc ccgcatgtaa gctaaaggtg cgatttcgat cacgcctcgt tccatcaatc
     8761 ggttcgtatg atccaatcca aaaatttggt ataaagcatc ataaacaggc cgcagataag
     8821 gatccacttt ttctttcaga tccccgggta gaaagcctag gttttcccct gcctccacag
     8881 cgggccgcgt caaaatgatt ttttgcactt cgcctttttt tagggcagca attgctaata
     8941 ctacggcaag aaacgttttc cctgttccgg ctggccctac accaaatacg atgtcatgtt
     9001 tggctaccga ttcaacgtat ttcttttgac cgctgttttt tacccgtata ggttttccat
     9061 tacggtcttt gacgatctct tcctcataca tctcaacaaa ataatctaat gtaccttttt
     9121 gccccatttt caatgccgtc acaacatctg gcgtacttac gtggattcct cgtttgatca
     9181 attcctgtaa tgtccgaatg actgaggcag ccaaggaaat ctgggattgc tcaccaatga
     9241 tctgaatgat ttctccacgt gtatggatgg ttgtttccgt tgtttcctcg atcagtttta
     9301 tatgcttgtc gtgtgcacct aaaagcatgc tgacatcatc agcagctgtc aatctaatct
     9361 ctaaagaact agactcttcc gtcaaaaata gacatcccct ttttttcgat tcattctttc
     9421 ctattttacc atactcagaa gaaactatat agcaaataag aaaaggttgt aaaagagtcg
     9481 cttaaccata cgtaccaaga aacgttaaaa aatagccgtt tccattcgct aaaggtctcg
     9541 ttttgataaa aagaagctga gaatgtaagt tatatgaaag tggagaacaa aatatttcgt
     9601 ttcattcaac aagtattctc taggtatttt tcctgtttca aaaaaagaaa acaaaatagt
     9661 taaaatattc ttgcatttca aaaaaaacag gtgtattatt acgtcaacaa ataaacatac
     9721 agcaaagcgt cgaggaaaac agtaggctat tcaacgattt attcccagtg agtcatgtaa
     9781 gtgagaaatg acaataaatc gaatagacga acatcatttc cgagtcaatg tcgaaaacgt
     9841 agatcattgt ggctgcctcc attatcaggc tagcggataa ctttatcaaa aagcgtccgt
     9901 gaatgaagaa gcttttttaa gcgaaaaaag gtggtaccgt tctagaatct agagcccttt
     9961 tcccaagtgg aaaagggctt ttttttattt taaggtggtg gttttcatgg aatctgatat
    10021 tagtggcatt actaattgct gcaacgttta tatcgtcaat caaaatgata attattggag
    10081 gaaaaattta tggaagaaac aaaaacttat tcatggaaac aactattgat ggtcagttca
    10141 cttatcttcg ggatgttctt cgggtcaggt aatctaatct ttcctgtcca cttaggtcaa
    10201 atggctggcg caaactgggt cagtgctggc gtaggatttg ctatctcagg tgctcttttc
    10261 ccattactag ctatcttagc tgtagtagtg acaaaaagtg atggtctgta tgacttagct
    10321 aaacctgtcg gaaaatggta tgctgccttg ttcttaatcc ttgttcattt gacgatcgga
    10381 ccatttttcg gtacaccacg gacagctgct actgcttttg aaatggctgc taaaccattt
    10441 ttgcctgaaa aatataattc attaggtatg tttttattct ctgctctttt ctttttactt
    10501 gcttatttcc ttactgtcca tccaacacgg ttggtcaaat acgtcggaaa gtatctaaac
    10561 tccttgtttc ttgcattatt agcaatcatt tttgtcgttt ctttattcca tccaatggga
    10621 aatctaaatc aatctgctgc agatacttat caaacaaacg ctttaacaaa tggtgtccta
    10681 gaaggttata acacggttga tgcagtagct ttgcttgcct taagtgtcac ttttgtccat
    10741 gcagtccgtg accttggttt caaagataaa aaagtcacag aattaacggc aaaagctggt
    10801 agcctgagcg ttgcgctaga agtcatgatc tattttggac ttgttcttct cggtgcgatg
    10861 agtttgaata agatgtcttt atcagaaaat ggcggaactg cactttctca gatcactaat
    10921 ttttatttag gtaacttcgg cgctagcttc ttaggagtga tggtcacatt aggtgtcttc
    10981 actaccgcta tgggactcgt tgtttctttt gcgcaagatt tccataaact gttccctaaa
    11041 gtcagttata tgacatggct gcggttaacg actttcgtct cttttgtcgt tgcaaatgct
    11101 ggtttagaca atatcatcca atggtcttta ccagtattga tgcttttgta tccattgtcg
    11161 cttgcgttga tcttgctttc cttaacagca aaatttttcc aaaagacacc atttgtctat
    11221 caagtaacga tgttgtttgc tgctgttcct gccgtattgg atatgctggc aaattcgcct
    11281 gctttagtga gtcaacaaag agttgtagca agcatgctcg aattttatca tcatcacgtt
    11341 ccttttgctg cattaggact tggctggatg gtcccaacgt tacttggtta cgctggcagc
    11401 ctactgtttt attacgccta tcgcctatca gggtataaac aagaagcaaa tgaactacca
    11461 gaagaataac caaatcaaac acaatttaac ataagaaata ttaaacagga aaaaaccagc
    11521 tagatgaaag aatcctattc tttcgtctag ctatttttta gttctctttt ttacgcgcac
    11581 aaaaaaactg cggacaatga ttgtccgcag tttaaatggg ctctataata tctagtgttg
    11641 gtgacgcctt ctgttgccaa tactttttct tttttctaat aaacgcaaac agacaccttg
    11701 aaatccttta gaataaagtc gacgaaaacc attcaaagga gtgtttcaag atgtcctatt
    11761 cagagtttac caaagagtta ctagatattc tagacctaaa tcttatcttt catgaagatg
    11821 cctttcgcaa agagcgaatc aatgacgaaa catgttttgt ttttgatggc acattgacct
    11881 atcaaccaga ggaatgcttc cattgtcact atcaaaataa gcaaacgatc attaaatggg
    11941 gctggaaaaa agtatccatc ttattgaatg atgtcagtaa ctacaagacc attctgcgga
    12001 ttaacaaaca gcgcttcaaa tgtaaacact gtggaaagac ttttttagca gaagattcag
    12061 ttagtgatcg tcgctgctcc attgctcgca gagtcaaaca agccattctt gagctattaa
    12121 gcgaacctct ttctatgtct ttgatcgcac gtatgaagca catttctccg accactgtca
    12181 ttcgaatact tcgcagcctt cggcctaaaa ccgtttcttt gaaccagccg ttaccagaag
    12241 tggtgtgttt cgatgaattc aaatcggtga aaaacgtctc gggagccatg agctttgtca
    12301 tgatggacgg caagacccat caactgattg atattgtgga aaatcggcaa ctaaatcctt
    12361 tacgcgacta tttcttgcgc taccctagaa aagtccgtga acaagtacgt ctagtggttt
    12421 cagatttcta tacaccctat cgcacattgg tgaaagagtg ctttccgaat gcccgaatcg
    12481 tcgccgatcg ctttcatatt agccaacaca tcggtcgggc ctttaccaac caccgaattc
    12541 aagtaatgaa gacctttaaa aaaagggatc gtcggtcgaa gcacttaaag aaatactgga
    12601 gactacttca aaagaatgcg tgggaattga aaggtcagca tcgctactgg cgtccgtcat
    12661 ttcgagatca tctgacagaa gcagagatag tcgatcggct attatcttat gatgactcct
    12721 taaagcgagg ctacgaagtc taccaggatt ttctttccgc tatcagaaga caagatgttc
    12781 ctgaattcgt cgcgctccta aaagaagact acaaggagtt gccagagcac taccaaccag
    12841 tgttcacgac cttcaagaaa tatcgaacag aaatcaaacg agcgttgcga gttccctatt
    12901 ccaacggccc tatagaatgt ttgaacaacc atatcaaagt actaaaacga attgcctatg
    12961 gctttagaaa ctttcaaaac taccaagaac gaatcttttt atatcgagga aaatatttta
    13021 agaaaactaa gaacactacc caactgacta aagctagaac aacaacacgg ctagacaaga
    13081 tagctgtcta gccgtgtgag tatctcgatt attctattga atttcggctc tatacttttt
    13141 gatgttggtg aacttcacca acatcttttt tgtataaaaa aatatagaaa agacaccctg
    13201 aaatccttta gaattaattt ggctaccaat tcataggagg attcaagatg tctcatactc
    13261 agcttatcaa agatacccta aatattctag acctaaatat ctatttcgag gaaaattgtt
    13321 taactaaaga aaaatacaaa gggcaaattt gcttgattta caaaggaact cttctgtaca
    13381 agccagaaga gtgttcccat tgtctttgtg tcgtcccctc taggatcatt cgttggggga
    13441 cgacaaccgt tcggttgtta ctgaatgatg tgtctgagta tcgcacttat ctggagctga
    13501 agaaacaacg atttaaatgt aagtcttgcc aaagaacatt cgtagcggat acctctgttg
    13561 ctgaaaaaca ctgtttcatt agtcaaaaag ttcgttggtc tgtcattgct cgcttaaaag
    13621 agaatacttc catgacggaa atcgctcgtc agaaaaatat ttccacttct tcggtctatc
    13681 gtgtcatgaa gcgattctac cgcccattga atcctttcaa acagactttg ccaaaggtct
    13741 tatgctttga tgaattcaaa tcggttcgaa gtgttgccag tgcaatgagt tttatcatga
    13801 tggacggaca aagccatgca ttattggata ttgtcgaaaa tcgacgcttg ccttatttag
    13861 aacgctattt ttctcggttt tcattagcga ctcgtgaagc cgttcaattg attgttattg
    13921 atatgtatgc gccttatgtt tcgttggtga aaaagctttt cccgaatgct cagttgatca
    13981 tcgatcgttt ccacatcgtc caacatattg gcagaacttt cttgaatcat cgagtgaaag
    14041 aaaccacggt acgacttaaa ggaaattccc actctcaacg agggttaggg aaaaaattga
    14101 agcgctattg gaaactccta caaaaagaag aagcaaagtt aaactatgaa aaacgggttt
    14161 ggcgtgccag ttttaaagat tatttgactg aaagtgagat tgccgatcga ctgttagctg
    14221 cttgtcccaa cttacgccaa ggctatcagt tataccaaga cattctttat gcggtaaaga
    14281 aaagagatca gtcactattt gaggattgtt taactagaga cattagcggt ctgccagaaa
    14341 cctatacaac cactttacga acgtttaaaa agtttctgcc acagatccaa aatgcgcttc
    14401 actacagcta ttcaaacgga ccattgga
//
LOCUS       DAJSKS010000066        14279 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJSKS010000066 DAJSKS010000000
VERSION     DAJSKS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.067"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(6..335)
                     /locus_tag="ON396_001656"
     CDS             complement(6..335)
                     /locus_tag="ON396_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HCR3113744.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            512..1240
                     /gene="sfsA"
                     /locus_tag="ON396_001657"
     CDS             512..1240
                     /gene="sfsA"
                     /locus_tag="ON396_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR3113745.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1256..2269
                     /locus_tag="ON396_001658"
     CDS             1256..2269
                     /locus_tag="ON396_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3113746.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2269..3546
                     /locus_tag="ON396_001659"
     CDS             2269..3546
                     /locus_tag="ON396_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR3113747.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3609..6311
                     /locus_tag="ON396_001660"
     CDS             3609..6311
                     /locus_tag="ON396_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3113748.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6463..6780
                     /locus_tag="ON396_001661"
     CDS             6463..6780
                     /locus_tag="ON396_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3113749.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6810..7130
                     /locus_tag="ON396_001662"
     CDS             6810..7130
                     /locus_tag="ON396_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR3113750.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7238..8698
                     /locus_tag="ON396_001663"
     CDS             7238..8698
                     /locus_tag="ON396_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3113751.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8766..8987
                     /locus_tag="ON396_001664"
     CDS             8766..8987
                     /locus_tag="ON396_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113752.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9018..9200
                     /locus_tag="ON396_001665"
     CDS             9018..9200
                     /locus_tag="ON396_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HCR3113753.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9200..9613
                     /locus_tag="ON396_001666"
     CDS             9200..9613
                     /locus_tag="ON396_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HCR3113754.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9736..10917
                     /locus_tag="ON396_001667"
     CDS             9736..10917
                     /locus_tag="ON396_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3113755.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(10988..11152)
                     /locus_tag="ON396_001668"
     CDS             complement(10988..11152)
                     /locus_tag="ON396_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113756.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            11306..11379
                     /locus_tag="ON396_001669"
     tRNA            11306..11379
                     /locus_tag="ON396_001669"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11340..11342,aa:Arg,seq:cct)
     gene            complement(11448..12587)
                     /locus_tag="ON396_001670"
     CDS             complement(11448..12587)
                     /locus_tag="ON396_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3113757.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(12886..13521)
                     /locus_tag="ON396_001671"
     CDS             complement(12886..13521)
                     /locus_tag="ON396_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113758.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(13634..14269)
                     /locus_tag="ON396_001672"
     CDS             complement(13634..14269)
                     /locus_tag="ON396_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HCR3113759.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
BASE COUNT         4582 a         2408 c         2848 g         4441 t
ORIGIN      
        1 gggatctatt tttcagataa caacttatat gaagtgtctg cagattcgtc ataaagagtt
       61 tgttcgggat aagaaaagat aagataaaaa ccatactgat cacgtacgac taaacgatat
      121 tcatctattt ctaccacaga tgtgccaaga gactgaccgt tgatcaaaaa cgtgtaatca
      181 gatgtaatct gtaattgttg tttttgctgg ctatcactaa agaaccatgt accttgatac
      241 tttgagagtc cttctgactt ttccgcttgt tttttctttt tcaagtaaat aaaacttgag
      301 agaacggtaa tgagtgaacc aagaaaatag atcatttttt ttatcaccaa atcactcctt
      361 tatgagaaaa taatcgcata gacatattct ttcttcttta tttcattgta ccgaataaaa
      421 gctaaaattt ctctaagact ttcgactggt ttttttactg tttttctaca aattatgata
      481 ggatacatag taggaagaaa ggtgacataa tttgaagtat cagcaagtgg aaatagcttt
      541 ttttattgac cgcccgaatc ggtttatagc acattgtatg aaaaaagacg gagaaataat
      601 tattgcgcat gtcaaaaata caggtagagg aaaagaggtc ctgataccag gcgctgaagt
      661 ggctattgta tttgctcctg gacctaaacg gaaaaccgcc tatgatctga ttgctgtaaa
      721 aaagaagggt gactggttta atatcgatag tcaattgcct aatcggctgg caattgatgg
      781 tattttagac ggcacgatcc ttttaccagg attaactggt ccatttacgg tatacaagcg
      841 tgaagtaact tatcttcact ccaaatttga tatttattta gagacggtgg tcggacaaaa
      901 agcatttgtt gaagtcaaag gtatgacact tgaaaataaa gcgattggtg cttttcctga
      961 tgcgcccaca ctcagaggat tgaaacatat aggagaatta acgtatgccg cacaagatgg
     1021 ttatgccgct tatgtcttgt ttattgctca gttcgaacat cttcatctgg caacgatcca
     1081 tgaagaaatg caaccagcac tagctgatat ggttcgacat gcgcagcaaa gcggagttca
     1141 aatcctcgcc tataattgtc aagtatcggc agatgaggta actgtcaagc aggccattcc
     1201 atttgatgta gatatccctt ttgaagatcc tatacaatag aaagaaggta aaataatgat
     1261 caacttaggt attatcggaa caaattggat tactcatcaa tttgtccaag cagctttaag
     1321 cacaaaaaaa tatgaattga cagcggtata ttctagacat ttggaaaaag cacaggaatt
     1381 tggttctcat tatgaagggg atatcgaatt cgcaacagat ttgaagactt ttttcaatat
     1441 tactcatatg gacaccattt atattgcttc tccgaatagt cttcacttta gtcaagctaa
     1501 gcaagcaatt ttggcaggaa aaaatgtgat agttgaaaag ccagcttttt cgacgccaaa
     1561 agaaatggat gaaatcatcc atttggcaaa tgaacacaat gtgtattttt ttgaagcagc
     1621 aagaaatatc catgaatcag gttttaaaaa gattgccgat tacttaccat taaaagacga
     1681 gataatcggt gcaaatttca gctatatgaa atattcttcg cgctatgacc aagtattaga
     1741 tggagaagaa ccgaatatct tctcgactca tttctcaggt ggtgcattga tggatttagg
     1801 tgtttacctt gtatatgcag ctgttgcttg gtttggaatg ccaaaagaag tccattattt
     1861 cccaagaaag atttctactg gtgtagatgg tcttggatgg gggatcttac gttatgagaa
     1921 ttttgatgta tcgatccagc caggtaaaat cggtgattct tatttaccgt cagagattta
     1981 ttttgactca ggaacgctag tgatgaatgg agtcaatgca attgaacaag cacaatttca
     2041 tgatcgcgcg cataaagaaa tcattgattt ggcggtaaca gctgcagaaa atccaatggc
     2101 agatgaagca gaggattttg ccaatgtcat ggctcatccg aaagatccag cttggggatt
     2161 gttgtatgaa gagtggatcg agctagctag aaatgtgaac caagtgattt atgatttaag
     2221 aaaaaatggc ggcatcaaat tcgatgcaga taatgaggaa gatttttaat gaacgaattt
     2281 gtacaagtat ttcccgaaaa ttggcagaaa aaatatgagg ataaagggtt tatctcacct
     2341 tctcttattc aacgagcagt ttttcagcca ctaagtaata aacaaagtat agtagcggtt
     2401 tctcctacag gttcaggtaa aacactggct tatctttggc ctctactact aaacgtggaa
     2461 ccaggtgaag caagtgcgtt ggtcattttt gcttcttctc aagaactagc tattcaagta
     2521 gcagatgttg cacgtgaatg gggaaaagac aaagagttga aagtccaatc tttggtgggc
     2581 ggagccaacg tcaaacgtca gattgagggt ttgaaggaaa aaccagaaat cttgattggt
     2641 acaccaggaa gaatattgga attgatgaaa gcaaaaaaaa taaaagcaca tcaagtgaaa
     2701 acaatggtat ttgatgaagc agaccagcta tttgacgctg ggaacagcca aatcattgat
     2761 caaattcttc atcaagctcc aacagagtat cagctagctt ttttttcagc gacagcagat
     2821 cgttcccttg aaagcattga aaaaatcact gggaaaacgt ttgagacaat tgacgtgaca
     2881 gcagaggacg attctcgaaa aggactgaga cattactttt tacgtgtacc gatacgaaag
     2941 aaagatgact acttgagacg tctcacgcat atagaagatt ttcatggtct gattttcttt
     3001 aatcaactga ctgaattagg cgtaatggaa gaaaaattgc tgtatagagg tgtaccggta
     3061 ggaagtctgg cctctgatca gaacaagcta ctccgaaaag cagcgctaga acaattcaaa
     3121 aagaaaatgg tcagagcact tttgaccaca gatgtggcgg ctagaggttt agatattaca
     3181 ggtcttcctt atgtagtgaa tgcagaagtt ccgttaagtg aagaagctta tttgcatcgt
     3241 tcgggaagaa ctggtcgaat gggtaatgaa ggaatagtaa ttacactcgt acaagataat
     3301 aatctaagag atttaaaacg gcttttacgt ccatcaaata taacacttga agagattttt
     3361 ttatacggag gtaagctgca aactgagcag ccaaccaaag aagatgcagc tcctgcagta
     3421 aataaaaagt atgcgaaaac tttttctgga aaggagcaga acccagaata taacaaagaa
     3481 aaaataagaa aaaaagttaa aaataaatca aaaaaagaga gaaataaagg agcaaggcgc
     3541 aaataagcgc ctttttatgc tatgattatg aaaacgggaa caaaattggt tgccagagga
     3601 ggggttatgt gaaagaacgt caagaagaga tcttgcagct attgacagaa aaccaacaag
     3661 gattgacagc aagtgatgta gctgagcgct taactatcga tcgaagcaat gcaagccgat
     3721 atttaagtga actatacaag gcccatcata ttgtaaaaac cgctggccga ccagtcgttt
     3781 actctttgcc aacggaaaag tcaaaatctg atgaagtgca tgtcgactct tctacgcaag
     3841 tgacttttga aacactcgtt ggagaaaatg attctttaaa agtcagcata cagcaggcaa
     3901 aagctgcgat tctttatcca ccgcgggggt tgcatacgat tatttttgga gaaactggta
     3961 cagggaagtc gatgtttgct gaatgtatgt atcattttgc aatcgattca gagatgcttt
     4021 ctgcagatgc gccatttgtt tctttcaatt gtgcggatta tgatcaaaat ccgcaactgc
     4081 tctttggaca catctttggg atcaaaaaag gggcttacac aggagcagct caagatagtc
     4141 ccggtctaat agccaaagca gacggcggta tattgttttt agatgagatc caccgtttac
     4201 cgccggaagg gcaggaaatg ctgtttactt tcattgataa aggtatttac cgtccgcttg
     4261 gagaaagtgc acaagtacat gaagcatctg ttcagatcat tggggcgacg actgaatctt
     4321 cagaaagttt cttatctacg ttcaatcgac ggattcctat ggcaatcacc ttgccgaatc
     4381 ttgcttcacg ttcactggat gagcgttatg aaatcatctc tctttttatc aaacaagaag
     4441 cgaatcgttt gaatcaacgt atcgatatcg aaaaagaagc gattcttgcc ttcatgcttt
     4501 atgatgcgga aggaaatatc ggtcaagtga aacgagactt gaaactagtc tgtgcaaaat
     4561 cttttttaca ttaccgtact catcatgaga aaaagctaat cattcgaaaa gaagaacttt
     4621 cccttcaagt acaaaaagga ttactgaaaa tcaaggaaat accggaacgt ctcgaccgat
     4681 tcatggacag caaaagtcag tatttgacat ttgaaccagg atttgctgat gttgtctggt
     4741 cacaagatcc agaacgaaac atgcaggtct ataacgatat tgaggaaaaa gtcacttctc
     4801 tttccgaaac cggtgtggag aacattgatc tagagaccct catttcaaaa gatgtggatg
     4861 cttatttcca gacatatgtc aaagaattga ctaaaagttc gattcccaaa gatcttttgc
     4921 cagatgaaat ctggcggtta accaatcaat tgtatgatgt ggctgaaaaa cggttagacc
     4981 gtatttataa tgaaaaagct cgttttgctt ttgctttgca tctgcaaagt acattggatc
     5041 gtgtgagaga aggtcatatg atcgtccacc cagatttgaa taacgtacgg aaaaatttta
     5101 agagggaatt ccaggtagca atcgatcttt caacgatcat tgaagaagaa gaacaagttg
     5161 agattccttt tgatgaaatc ggctttatta gtatgtttct ttctatcaat ctaggagaaa
     5221 gtgagcctgt taaagaaaac aaagtagatg ttctcatatt gatgcacggc agacagactg
     5281 cttcaagcat gttggagatg gcacaagaac tattaggaac aaaagtaggg aaagctttta
     5341 atatgccact ggttatggaa gttcaaacaa tgtatgaaga agtgttacaa tatgtcaaaa
     5401 actatcaaga gcatttgacg aatggcctac tcttgttaac agatatggga tcattaaaca
     5461 ccttcagtga tttgatttat gaagagactg gcattcggac gaaagctatc acgatgacaa
     5521 gtaccatgat tgtgatagaa gcgatacgga tggcaagtgt tggaagaagc ttagaagata
     5581 tttatcaaaa tatccagctt tcctttgaat cgatcgtacg tgatcagttc aaagcggaaa
     5641 agtctgaaac tttacagcgt aaaaaagccg taatcgtgac atgctttact ggtgaaggag
     5701 tagctgccaa attatatcag cgtattatgc cagtgattaa tcaaaataag gtagaactga
     5761 tccagatgca gtttttagaa cgtgaatcat tcaaaaaaca catcgatgcc ttattggaag
     5821 aatatgagat caaagcaatt gcgggaacag ttgatgttga ttatcaaaat atcccatttt
     5881 tttcagccta tgacgttttt gatgatgaaa aactaaatat tttaaagcgt atagctgatg
     5941 atgagatccc gacagctaaa attgtcgatt cacttgctgg atcattaagc gatgtctttt
     6001 ctattgatga actgatccgt tcattgcaaa agacagttca gcagatccag accgatttgt
     6061 cgattatcgt ggaacctgga gtagatactg gaatcgttat ccatttagcc ttcatgttag
     6121 acggattgat caaacaagaa agccatcggc aatttgagaa actgtctatt ttctcaaaaa
     6181 tttatcgttt ggaaatggat atcgttcggg caaacttgat ggcactagaa cgaaaatatc
     6241 aggtaaaaat tccagaagat gatatcgcat ttcttacaca aatgtttctt gaaaataaaa
     6301 taaaaaaatg aacacactaa tcatactgtt tacacactaa acatctgtgt gcgcactata
     6361 aaaaagagct aaaaagcatg aaaacgctat ttcagctctt ttttttattg gcatgcttct
     6421 tgcgttatat aaaagtgtaa aaaaataaaa ggagtgttaa aaatggcaaa aaaaacaatc
     6481 atgttagttt gttcagcagg gatgagtacg agtttattgg tcacaaaaat gcaaaaagca
     6541 gcagaagaaa aagggatgga agccgatatt tttgcggtct ctgcctcaga tgcagataat
     6601 aatttagaat ctaagaatgt cgatgtatta ttattaggtc ctcaagtacg tttcatgaaa
     6661 gctcaatttg aacaaaaact tgcgcctaaa ggtattccat tagatgtcat caatatgcaa
     6721 gactatggca tgatgaacgg agaaaaagtt cttgcacaag cagaaaatct aatgaaataa
     6781 atcaagtatg cagttggggg tataacaacg tggaagatca acaaaatcta gaagccatca
     6841 tggggttaat catgtttggc ggtaatgcaa agagcgatgc aatggaagcg attgcggctg
     6901 ctaaaatcgg tgattttgag ttagcggata ggaaaatcgc tgatgcagaa gaatcactta
     6961 tacaagcgca tcatgcacaa acagacatgt tgacacaaga agcacaagga aatcatatgc
     7021 aagtgacatt attgactgtt cacagtcaag atcacttaat gacatctatt gcgtttactg
     7081 atttagcaaa agaaatcatc gaactttatc gtcgcgtata cagcgaataa atacaacttc
     7141 ttttttgaac ggaagaagaa aaatttttga acggattttt tattttgtta taaggagaac
     7201 tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat caagttggat
     7261 ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt tagttgcttt
     7321 gcgtgatgct tttatcggca tactgcccgc aacaatggcg ggatcagtcg ctgtaatgat
     7381 caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg acagtttagc
     7441 agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg gttttgtctg
     7501 gaacggtaca ctagcaatcg ccggattgat ctttgccttt tcttgggggt ataatcttgc
     7561 ccgagcttat ggagtaaatg atctagctgg ggggatcgtt ggtttagcta ccttgattca
     7621 aggtatttcc tttggtccag cattatcagc agcattagat gtgaatgttc cttcaaatgt
     7681 cgcagatgca atcaatagtt ctgagattgg tgcaacgatt gcagatggca cactgtcagt
     7741 tggattatgg ggttggctaa aactagatca tttgaatgga aacgcttatt ttacagttat
     7801 gatcatggga gctatttcag tcattatttt ttgtaaattg atgcttgcca acattacgat
     7861 caaaatgcct gattcagtac caccagcagt atctaaagca tttgcagcca ttttaccagc
     7921 gacgatcgct ttatacgtca ttgctattat caattatatc gtaggtcgtg tagctgatgg
     7981 gcaactgatc attgatttgg tgcaaaaata tattgcagaa ccattcctag gattatctca
     8041 agggttggga gcagtattga tcgtcacagt atttgttcaa gtattctggt tctttggtat
     8101 tcatggatca aatgtattgg caccagtgct agagggaatc tggggacaag cacaactaat
     8161 caatattgat attttccaaa aaggttataa cggaaaaact ggtacaccag ccgtgttaga
     8221 tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg cttttgcatg
     8281 gtttggcgga tcaggcggaa cgatcgtctt actgatagct atcttgattt tctctaaacg
     8341 tgcagattat ctaacagtag ctaaattatc cattgggcct ggtatcttca atatcaacga
     8401 accagtgatg tttggtctgc caatcgtatt gaatgcaatc atgtttatcc cattcttgat
     8461 tgctccagta gtagcaacag caattggatg gacagctact tatcttggcc tagtcgctcc
     8521 ggtatcgcag caagtcacat gggtagtacc gccgttctta ttgtcattct tagcaacagg
     8581 agcggattgg cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt tcttgatttg
     8641 ggcaccattt gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac aacaataaga
     8701 tgaacgtgag gagcgggacg ctttaagttt cgttcctcat aatgaatttt cgaggaggaa
     8761 ctgaaatgat tacaattgcc agtgcaatgc aacaagatga aatcaaagaa ttgttagaag
     8821 gatacaatga agaaggaatg acttttacat ttaaagaaaa acaaggaatc aagctgttct
     8881 ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa ctaatcaaag
     8941 cacagccttg gggaacagtc ctttattttc aatcaacagc tgtttagaaa atggtttaaa
     9001 aagataggat ggttgagatg ataaaaaacg gtttattttt atgtagtatt ggttctttga
     9061 tcgtgttgta tagtttgaat ccacattcgc aaaggtatga tttgtatagt atggtcacag
     9121 gtctttttct aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa agagaagaag
     9181 caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag catcgttttt
     9241 ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg gcaaatcagt
     9301 aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa ataaccgggc
     9361 tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta catcgacaac
     9421 gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga cctgtgaggt
     9481 tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg atatgctttt
     9541 aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag atatgatcga
     9601 agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat gaagtaagct
     9661 agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag cttttttgta
     9721 ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc gattgaggaa
     9781 atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag gcgagaacgt
     9841 tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga ggatcggcta
     9901 acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga gtacctaatg
     9961 gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg gatcaatcag
    10021 atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc ttatctggcg
    10081 ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga taaaatcagt
    10141 tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa aataaagcgt
    10201 atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat gccggaaaac
    10261 caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa gcaaaaaggc
    10321 aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata tatcgtttat
    10381 cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca tgatgctttt
    10441 tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt tgaatattca
    10501 tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc ttctgtaatg
    10561 atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga aaattatcca
    10621 tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta tgcgaaatgg
    10681 aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc aaaagtagaa
    10741 caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct attattaggg
    10801 tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa agaatatgaa
    10861 gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta tttttgataa
    10921 aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg gcatttgaag
    10981 gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt tcttttgttt
    11041 tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc cgattttttt
    11101 tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc attggaaagg
    11161 acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac tagtggaaac
    11221 gttttgccta acttataagc tgattttttt agcagataag cttgcaagta aaaaacaaac
    11281 aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag caatcgcctc
    11341 ctaagcgata ggtcgctggt tcaattccag tcaggaacgt ataaataacc cctgtatcct
    11401 ttgcggtaca ggggtttgtt ttgtatttgt gccaaaaatg tgccaaacta gttatcattt
    11461 ttatataaac tatcgaacac agtattgatt ttttctttat cctgatcttc aagctctcga
    11521 acaatatgaa gataagtaga cattgtagtt tctaaactgc tatgtcctaa acgtttcgat
    11581 acgctaagta tatttactcc ttgataaagt aaaatagatg catgtgtatg ccttagtcca
    11641 tgcaaagtaa attgcttttc aatccctaat tcttttaatt ttttgcgcaa atatttgctg
    11701 acggcgtttg atgaaactaa tccatttttt aagttgaaaa aaacaaaatt atgagggttt
    11761 ttgatttcaa aattctcgaa taattctttt tgattgattt taaacttttt taagagatca
    11821 atcgtatgtt tatcaatgga aattttccta tttgaagttt cgttttttgt ttttccccat
    11881 tcatttaatt tataattcca tgttttatta atagtgatga tttgatcttc gaaatcgata
    11941 tcttcccatg ttaatcctag aagttcagca aatcgcattc cagtggcacc agcaactaac
    12001 acgagcatag gagaagagta ctgcgcagaa aggttttctt ctacgagttt cattaaagat
    12061 ttaaattgat cataatctaa atatttatct tcttttggct tcaatgaatc ttttcctttg
    12121 attactgctt ttctagttgg atcaaacgga attaatcctt cttctacggc atctttcaac
    12181 gacgctctaa tatggttatt aaacttaata acggtagatt ttacatggtc tttagcatac
    12241 ttatttaaaa agcgttgata tcccactcta tctaaatcag aaatcaatac cgctggcata
    12301 tatttcttta tgttcattaa cgtatcttcg tattttctat aagtgatagg agatactgtt
    12361 ccttctttat aaagttgcat ccaatcctca aagtaatctg ataataatag atttttgcga
    12421 tcccctttaa ggcctttgtt caagttatat tctaattcgt tggcggcatc ttttgcttct
    12481 ccttttgtct taaatcctga ttttcttatc ttcgtgtatt ttccgtcatc ttttttgtat
    12541 gaaatttcgt attgccaact atttccacgt ttgactaatc ttgccataat tgaatcagct
    12601 ctctttctct gatacaatag gaactataaa gaagcccatt gtttgggttt tattattttt
    12661 tgcacatcct ttttgcttgc cggcgcaagg atgtgctatt tgtcgtttta atggtataat
    12721 tggatgtgta ttagatcatc caatcagagg tgtttattat gattaaagtg atttttaaaa
    12781 gaataactga acttccagag tttagattat ttgttgatta ttggtatgtc tggtttttta
    12841 ttgttgcatt gctgataggt ctagatgtca tattagaaag aaaacttaag taagagttga
    12901 gttaataaat tgaattccta aatgagatat tttgtagccc atattttgtt tgccgacatc
    12961 acctaatact atggagctaa tcgttttttc taacagacca aaatcagata atctatttaa
    13021 gctaccatct attagctctt gtgaatagcc agacatgctg atatttccag tgaaattttc
    13081 gtttccacga ctcattaagc atgctaacaa ttgaatctca atcgatgtta attgattcag
    13141 tacaagaaga taatactcct cgttatccca tgaagcgttg ttaattcgaa gtaaacaatt
    13201 cttataaaga tttttataca tatctatttt attttgtgcc tttgattttt ctacctcgtc
    13261 atttatccct tcgaatattc caatgagttg gtccttgttc tctaagttga aaacatcttc
    13321 tggaatttga tcgcgaatgt tttctaatga ttcgtttaaa tttttgtaga atttctcgat
    13381 gcgtttgaaa cgcttctcgt ttttagaccc aaaataaagt gactgcacaa cgccaccaat
    13441 ataaggaatt gctccaatac cgccttcaac tattaattca agatattctt ttttggttag
    13501 tttttcattg ctattatcca tagttacctc ctattttgtc atttccttta caagcattgt
    13561 gactcctgat ggcactcttt tgtataggtt ttattattgt gcacaatcta actcttagtg
    13621 gggcgtggtt catttatttt ttacttactt tactattaat catatcccat tgagcatagg
    13681 atttttctat gttcgatcct cttgaactaa tttctcctgt aaaagttact ttatcgccct
    13741 ttgtaaaggt atcagcattg tttaagtcct tcgcaaaaat tacatatgaa actttaggtg
    13801 ttgcagaaat atcattccaa cttttatcat cgtactcttt gtctgctata atggctattc
    13861 tagtagtcat tgattcgata acagttcctt caaaagtata tgttttacca ctaatatatt
    13921 tagtaaaagc tgtattttgc tcagtagctg gagtaataga ataataacta tctaagaaag
    13981 caccccagtc atgattattt gtctctaaaa attcttcaaa ttgagttagg cttttattgc
    14041 tgctgttaga tgtagtagtt tgagtatcag atgatgagct ttcctgtttg ttattgtcat
    14101 ttgcctcgtt tgatgaggaa agcgagaaac ctacggtagt aaaaataatc ccgataataa
    14161 ggagaatcat aggtgttttc ttatttgttt ttttgataaa tcttacaatt aacatgatta
    14221 aacctataat aaataaaaca ataccagtaa aaccaaatat accgtccatt aatagatcc
//
LOCUS       DAJSKS010000067        13808 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJSKS010000067 DAJSKS010000000
VERSION     DAJSKS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.068"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ON396_001673"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ON396_001673"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR3113760.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ON396_001674"
     CDS             complement(2261..2512)
                     /locus_tag="ON396_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HCR3113761.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="ON396_001675"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="ON396_001675"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCR3113762.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="ON396_001676"
     CDS             3462..3680
                     /locus_tag="ON396_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113763.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3969..4481)
                     /locus_tag="ON396_001677"
     CDS             complement(3969..4481)
                     /locus_tag="ON396_001677"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR3113764.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="ON396_001678"
     CDS             complement(4495..6792)
                     /gene="recJ"
                     /locus_tag="ON396_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR3113765.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6934..7377)
                     /locus_tag="ON396_001679"
     CDS             complement(6934..7377)
                     /locus_tag="ON396_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HCR3113766.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7378..8166)
                     /locus_tag="ON396_001680"
     CDS             complement(7378..8166)
                     /locus_tag="ON396_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3113767.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8163..9104)
                     /locus_tag="ON396_001681"
     CDS             complement(8163..9104)
                     /locus_tag="ON396_001681"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCR3113768.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9281..9868)
                     /locus_tag="ON396_001682"
     CDS             complement(9281..9868)
                     /locus_tag="ON396_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HCR3113769.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10089..11396)
                     /gene="obgE"
                     /locus_tag="ON396_001683"
     CDS             complement(10089..11396)
                     /gene="obgE"
                     /locus_tag="ON396_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCR3113770.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11690..12700
                     /gene="gap"
                     /locus_tag="ON396_001684"
     CDS             11690..12700
                     /gene="gap"
                     /locus_tag="ON396_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR3113771.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12785..13720)
                     /locus_tag="ON396_001685"
     CDS             complement(12785..13720)
                     /locus_tag="ON396_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3113772.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3096 c         2435 g         4469 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aagaagttaa
     3721 acttttacgg gggtgtgaca aaaatcggtc acatcctttt tttcgaataa acggtgttct
     3781 aaagccagtt tttcggtatt aagtcaaatt gcggaaaaag tgatttttta aacttctact
     3841 ggtctttgct tgtttccatt tataaaaaaa tagcgaccct caaaagtcag atagagaatc
     3901 tgacttttga gggtcgcttt aaaatcgctt ctttaagaat aacatcaaga atagcttctt
     3961 ttttctgctt aatattccat taatgtaatg atgtcatatc ctttgatttt gtcgcgtcca
     4021 tggagatcca tcaattcgat caaaaatgcg cagcctacga ctacaccgcc taattgttcg
     4081 atcagttcga tcgtcgcttt gattgttcct ccagtagcta gcaaatcgtc acaaatcagc
     4141 acacgttgac ctggtttaat ggcatcctta tgcaatgtca atgtatctgt tccatattct
     4201 aatccataag tcacttcgat ggtttctcga ggtaacttcc cttttttgcg gactggggca
     4261 aaaccgactc ctaactcata agcaacagga catcctacga tgaatccacg ggcttctggc
     4321 ccaactacca tatcaattcg tttttctttt gcataattta cgatttgttt agtagcttcc
     4381 cgataagcgt caccatctgc catcagtggg gaaatatctc gaaacacaat tccttttgac
     4441 ggatagtcgg gaatactggc aatataatct tttaaatcca ctctttgttt cctcctaggt
     4501 tgttaaccat ttctttatgg taggaatatc gctcaaaagc aaaaattcct caactttgat
     4561 tttattgagc cgtcgctggt ataatctgct ctcagtcaaa ggatgacttt ctgggctagc
     4621 gactttttgt aaaacaccgt tctctattgt aacaaatttc aattcaaaaa acacctgtat
     4681 cataaaaatt aataattttt cagggatacg caaatattga gctattgctt ttattttatg
     4741 acgtatatca attcgttctt gagaatggat caatttaaac aattttgcgt actgttctct
     4801 tgtgcctacc ccattcaaat aggcctcatc aggtgaaatc cccatcagat aaacacgatt
     4861 gaatgaataa gaccgaaaaa cttctttcag atccgctaaa tcatctggac aatccataac
     4921 aactagagag tttggttttc ttttttcaat tattgccttt acagaatcta gtccttcgaa
     4981 aacaatcgca gcttcctgga taatctcagg cacgaatttc aacgtcgaag aatcaaatgc
     5041 taaataaagg ctaccttccg acgttcggct ctgttcacgg aaacgttttg cccgccaatc
     5101 aaatacttgc agaccaccca cagaaaaatc agataccatc agctgcggtt tcttacgccc
     5161 gttccattca ttgatcgaaa gctttcctac cacatctacc tgattattca gcagttcttc
     5221 ttcttgagta ccaaaaccaa aagcgaccgc atcaagctga ctcgatgcat cggaaaggac
     5281 aaatttcaga tgctgttggt ttgctccgat tcttttgata ttttctgctt ggacttgtcg
     5341 aaataaaaac tgaggcaaag gattatccgt accaaatggc gccaatagtt tcagctcatc
     5401 aattcgctct actgttactt catttggcag caatacttca tcaattctca actcaggccc
     5461 tctcatcaag tcaatttgat gatcgactat atattggttc atcttctcct gtagtatagt
     5521 gacattttca cttggcatcg ttaaaccgac tgctgcatga tgtcctccga agtaagtaaa
     5581 taaatctcgc atctggtcaa gcatttcaaa aagattcaag gcttcaatac tacgtccaga
     5641 tcctttagct gatccatctt cttttaaagt aaggacaagc gttggtttac ccgtttcatt
     5701 cattattttc ccagcaacaa tgcccaaaac gccttcatgc catcctcggt ttaccaatag
     5761 atggatatga ttctccttat tgaccatcgc taatgcttct tgtgttatct gctctacgat
     5821 tgcttttctt tcttcattga tctcattcaa tttttttgct tgaacggatg cttcctcttc
     5881 atcaaacgtc gcaagtaacg agacggcagg acgtggatcg cccatccgac cgattgcatt
     5941 tagtctggga gcaatcgaaa aaccgatcgt tgtttcgtct gcgtccctca tttttacgcc
     6001 gctttcttca aatagcgcct gaagtcctat ccgttctgaa tgatgaatgg catcgattcc
     6061 taatgaaaca aggattcgat tctcatcagt caatgaaact aaatcggcga tcgttcctat
     6121 cgctactaaa tccaaaaatt ctgcaggcgg ttcttccaat aatgcagaag ctactttgaa
     6181 tgctactccg actcctgcca aatctggaaa tggatagtgc cccgctggat gacgagggtg
     6241 aacaatcgca taagcgtctg gtaaattctc aggtagttcg tgatgatctg taatgatcac
     6301 gtctacacca gcttgctgag cgtatgctac cgcatcattt cctgctaccc cgttatcaac
     6361 agtgatgatc aactggattc cttcttcgat cttttcttgg tataccgatt ggtttggtcc
     6421 atacccatgc tcaaatcgat ttggcaggaa tacttctact tctgcaccaa gtaattccag
     6481 tgtttctttc attaccgttg cactggtgat cccatctgcg tcataatcac cgtaaacgag
     6541 gattttttct ccactgatta cagcttcttg gattcggctg attgctttat ccatatcgtg
     6601 tatcagatac ggatcatgta attcttgttc ctttggatgt aaaaatcggt tgatatcctt
     6661 ttcctcatgg taacctcttt gccacagtaa cttaccgaca aatggagata atttcagttc
     6721 acggatcatt tggagaaagg attcagaagg ctctgtctcg cttaacttcc aatcaaaatt
     6781 tgcttgtctc actttctcac tcctaactta gttattatag caaaggaaat cagcaaaaaa
     6841 aagcagaaag aaagaataaa aacagatcag atccgctaga cagatgttca gcaggacctg
     6901 atcttttgta cgaccattta tgaaatatcc ctattagtca aaataattga agctttgttt
     6961 gggcgattga gttttttgtt cttccggcgc tttcaattga ctgatttgtg cggttttcgc
     7021 ttgtaactcc gcctgataag cagcttccaa ctccgcacgt tcgtttctaa actcgcgttg
     7081 ctgttcttct tttgcttctt caattttctt ttgaagttct gtttgatatt ctgacatttc
     7141 ctgagtcagc tgtttgattt gctttttttg ctgccatagt gttgccgaag cagtcaaaaa
     7201 tacaatcagt gcaccaatca gcgcagaacc gatgatcact aagatcaatg gcgcagaaaa
     7261 ggaagtgaag ccaaaactta caggaacatt tttattgttc aatacagcaa aaagaacaat
     7321 aatcaaaact aaaacaaatc caagaatcac attgctttgc tttttcaatt ccttcaccta
     7381 ctttttgtta aaaattccgc tggctaaata atcaccaatc gttggaaaca gcgtataaaa
     7441 ttttgatgca acttctaaaa taaacggttg attgatttcc cgttttggat gtctcatatt
     7501 gcggacgatc atttttgcca acttggcagg ttctaaaacg atttgtccga ctttttccaa
     7561 atagcttccg cttggatcag ccttatcgaa gaaatttgtc tcgattggac ctggattcac
     7621 tgtcgtaaca gctacgccaa gtggtttcaa ttctaatcgc aaagcgttgg agaagccaag
     7681 gactgcaaat ttcgtggcag aataaaccgt tgattttgca gttgccattt ttccagccat
     7741 agaggcgata ttaatgatat gaccttgtct tctttctgcc atatcgatcg caaatttttg
     7801 tgtcaaaacc atcattccta agatgttgac ttcaaacata tcgtatgctt ttccaaggtc
     7861 gaatgtaagg aagtcttcga aaatcccaaa acccgcattg tttaccagaa tatcgacgcg
     7921 cccgacttct tccatcacct tttcatataa ttcgtccacg ctttcaggat tgctgacgtc
     7981 taccttgaaa gcataagcct ctttgccgct caagcgtctg cactcgtctc tgacaccttc
     8041 tacaaatgcc attcttcgtg cgcaacttat aacgattgct ccttgctttg ctgcttcata
     8101 acagatttgt gcgcccagac cgcctgaact acctgttact aaaacaactt tatcttctaa
     8161 attcatgcct cttcctcctc gcgaaatgga atctcaatgc tatcaaaatc tttcataatc
     8221 gtcgtgtttt caaacacttc ttgtgcttct ttttcaagct ctaaagctgc ttttcctaaa
     8281 tatctcgcac tgatatgagt aagtagcagc cgttttgcct gcgcttcttt tgcaacttca
     8341 gcagcttgat gagtcgtgga atggaaatag ttgtgcgcca ttcttgcttc atctttatta
     8401 aatgtgcttt catgtaccag aatatcgcta ttttccgcaa gcacaacgga attatgtgtt
     8461 tttctcgtat cgcctaaaat agtcacgatt cgtccttttt tatctggacc tacgaaatct
     8521 tttccattga tcgtccgccc atcttctagt tggatcgttt caccttgctt cagtttgccg
     8581 tacagtggtc cagctggaat acctaattct tttagacgat ccacctgcaa ttctccttta
     8641 tgatcatgtt caaccacacg ataaccaaaa ctgtcgatcc catgatttag tttttttgta
     8701 taaacagaaa attgctgatc agtaaaaatc ggggctgttt catttagctc aatgaatttc
     8761 agcggataac ttaaacgcgt ttgcgaaata cctaaagaga cttttatgta ttcctctatc
     8821 ccttttggcc cgtaaatctc taatggcgta tccccacctt gaaatgaccg gctgctgatg
     8881 agcccgggaa gtccaaaaat atgatcgccg tgtaaatgtg taatgaaaat tttaccgatt
     8941 tttctcggac gaatagtcgt tcgcaaaatc tgcatttgcg tgccttcgcc acagtcgaac
     9001 aaccaaactt cattgcgctc gtctaaaagt ttcaatgcta tacttgtcac atttctatgt
     9061 tttgcaggaa cacctgcacc tgttcctaaa aattgtaatt ccataatcta cctgactttc
     9121 tttaagttcc tctttaattt tagggtaaaa agaagggata atcaaccgta agtcgagaat
     9181 gggttcaaat taaaaataac gttctaaagc gatagcgttt tctttgaaaa ttactataaa
     9241 aacagcagcg aacccgtttg agatcctctg ctgtttttta ttaatttgaa gatccagaac
     9301 tttcattgtc gttggaagtt tgcggatgtt tgtccttcca agtcttttgt gcagcttctt
     9361 tcgcatgatt ttcagcttct ttcgcatcat gcacatcttt ttcgacatct tcataatcca
     9421 agcgagtaat ttctccgcct aattccatca ttaccaattg attcagtggg cgattgtcct
     9481 cttcatcagc aatcaagatc agtcctgtag ctccttcacc gattttattt cctacaaagt
     9541 cgaacacggt ctgggcatct tttatttctt tggcatcttg tgtcgcaccg atcattcctc
     9601 cggcaaacca tcctaataaa atccctagcg gaccacctaa tacaccaacc aacattccga
     9661 ttaatccacc cgtagaagat ttgttattac cggtaaagtc gataaaatct tctattttga
     9721 attgatgtgt cccattagga tcatggctga caactgccat ctgttctcct ttaatcgaac
     9781 gaacagcatg taactttttg atttctgaaa atgcctgata tgcctggctt tctacttgaa
     9841 aatttaaaat gattactcgt ttgctcatta aaaacgcccc tttcatacct tcttgttatt
     9901 tattttacct ttaattgaaa aataagaaaa gaaaagcact ctttttttcc aataaaatag
     9961 ggctgtgaca aaagtcgtat cacagctcct ttttccgaat aaacggcgtt cttgaagctg
    10021 atcacttctg tcacaacctc ccttaaatct gaatctatat tttttggtat ttgtttttat
    10081 ttgcttgcct attcgacaaa ttcaaattca aactcaccga tacgtacaag gtcgccatct
    10141 ttcgcaccgc gtgcccgaag agcttcatcc acacccatac cacgtaattg acgagcaaaa
    10201 cgcatcactg tttcatcatg atcgaaattc gtcatttgga ataatttttc gatcttctca
    10261 ccagataaga tccacgttgc atctgaatca cgatcgattt ggaactcagg accttctgga
    10321 ttgaatccat agtgtacagt ttcttcttcc agttcttctt cgtacaatgg gaattctggc
    10381 gttacttcta acaaatctgc tgtagcattc aataacgcgt ccaatccttg tcgagtcact
    10441 ccagaaattg gaaatactgg gatgtcatca gcaaattcat cttctttttc cttgttcagc
    10501 tgttctttga atttcactag attttccggt gcatccggca tatccatttt gtttgccacg
    10561 atgatttgcg gacgctctag caagcgtaag ttataagtag ataattcttt gttgatggca
    10621 agatagtctt cataaggatc tcgtccttcc atcccactca tatcgatcac atgtaaaatt
    10681 actcgtgttc tttcgatatg acgaaggaat tgtgtaccca gcccaacacc ttgcgaagct
    10741 ccttcaatca agcctggcaa gtctgctgcg gcaaaacttc tgccatcact tgtagtaacc
    10801 attcctagat taggtacgag cgtcgtgaaa tgataagcgc cgatttttgg acgagcagat
    10861 gaaataacgg atagtaaagt cgatttcccg acagatggaa aacctacaag tccaacgtct
    10921 gccaatactt tcaattctag ttcgattttt cgctcttgtc ctggttcacc gttttcagca
    10981 atttccggtg caggattccg aggagaggca aaacggatat tccctcgtcc accgcgtcca
    11041 ccttttgcca caactagtgt ttgaccattt tcaattaaat ctcctaaaag agcaccagtc
    11101 tccgcatctc ggacagtggt tccttgtggt actttaacat aggtatcttc tgaaccacga
    11161 ccgtgcatcc ctttactcat cccattttca cctggctgcg ctttgaaatg gcgattgaac
    11221 cgaaaatcca tcaatgtacg taacccttca tcgacgatca agatgacatc gccgccacga
    11281 ccaccgtctc cgccagccgg tccgccgtca ggaacgtatt tttcgcgacg aaatgcaacc
    11341 attccgtctc cgcccttgcc agctttcaca tcgatcgtta cttgatctaa aaacatggac
    11401 ataattaagt cctcctatta ttcatttatt tatttgactg cctgaatttc cacaataaac
    11461 aaaagtctta cttattttat cggtgaaatc tgattttgtc tatcatttca gcaaaaagat
    11521 tgtttacctt cttctttttt tatcatttgg taaggtttcc aacctttttt ctgctaccaa
    11581 gtataatttt ttgacaaaat attctttgcg tagcagaata acagtgtatt aattgtatta
    11641 attgaggatg ccgtttcaca gcacccctaa taaaggagga acttttgata tgacagtaaa
    11701 agtaggtatt aacggatttg ggcgtattgg ccgtctggct ttccgccgca tcaaagaagt
    11761 ctcagatgat attgaagtag tagcaatcaa cgacttaaca agtccaacta tgttagcaca
    11821 attattgcaa tttgactcta ctcatggtac ttatcctggc acagttactg caacagaaga
    11881 ctcaatcgta gtggatggcg aagcaactcg tgtttatgca gaacccgacg caagcaaaat
    11941 cccttgggca aaagaaaacg gtgtagatat cgtactagaa tgtactggtt tttatacatc
    12001 tgaagaaaaa gcaaaagcgc atttagatgc tggtgtcaaa cgagtagtta tttctgcccc
    12061 agctggtgct atgaaaacca tcgtctataa cgtcaatgat gatacattag atgaaaacga
    12121 caaaatcatc tcagcaggtt catgtacaac aaactgttta gcacctatgg cttatttctt
    12181 aaataatgaa tttgggatcg aagttggtac aatgacaaca gttcatgcct acacttccac
    12241 acaaatgctc ttagatggac ctgtaaaagg cggtaatttg cgtgctgctc gttcagctgc
    12301 tgacaatact atcccacatt ctactggtgc tgcgaaagca atcggcttag ttatccctga
    12361 gctacaagga aaattacaag gacacgcaca acgtgttcca gtcgttgatg gttcattaac
    12421 tgaattggtt tcagtattga aaacaaaagt aactgctgat caagtaaacg aagcaatcaa
    12481 aaaacacaca gttgacaatc cttcattcgg gtatgatgac cgtcaaatcg tttctagcga
    12541 tgtcattgga acaactcaag gttcgatctt tgacccaacg caaacagaag taacaactgc
    12601 tggtgatttc caacttgtta aaactgttgc atggtatgac aatgagtatg gatttacttg
    12661 ccaaatgatt cgcttactag aaaaattcgc taacttgtaa tttgatagat caatcgataa
    12721 aagaagcctt tgactggatc cagtcaaagg cttctttctt ttcatcccct acttttaaac
    12781 tcgttcaatg aacatattgg cttaaaaatt gttggactac ttgctgatat ttttcaggat
    12841 ctgtcttgta tgcttccgca tgctcggctc cttttacaac atacttttct tttggtccct
    12901 tcgttgcacg atagacctta tctaacattt caaatggtac aaatgtatct tcgtctccat
    12961 gtataaagag cattgggcgc tggtttttct tcaattgttc cactgcattt gcttcaccga
    13021 aaaagtagcc agcacggact tttgtcacta gactcgttac ttgcataagc ggaaatgctg
    13081 gaagactaaa catatcgtct agctgataag caagctcatc atgaacggaa gaataaccgc
    13141 aatcttcaat gattgctttc acattatctg gtagtttttc tccgctagtc atcataactg
    13201 ttgctgctcc catgctgaca ccgtatagaa cgatttcttg cgattttcca ttgttttcta
    13261 gcactttgtt tatccactgt acatagtctt tacgctctgg ccatccaaat ccaatgtaat
    13321 ccccttcgct ttttccatgt cctcttgcat caggaacaag gacattgtaa cccatatcat
    13381 gaaacatctt tgcgtaattg gtcatcgttt cagcgtttcc catatagcca tgagccacaa
    13441 gagctgtttt ttcacttttc gtttcagcag gaagataaat cgctgataat ttcagaccgt
    13501 cttttgaagt gatggtccaa tcgcttcggt tttcttttga actaaaccat ttatgtgcat
    13561 ccgtttcttt ggatgtctgt gttgtaccag gtgtatcttc gtctaaaaag tctttctcag
    13621 aaggcacaac agcataatta taaaaataat tccctgcaaa cactaacgca acaatcagag
    13681 ctaaagcgac gcctaaaagg attttcatcc aaagtttcat aaatcatcca tctcatttct
    13741 cttttttcat ataaccttga attagtataa tcaaagaatc tctactatgc aatgttcatt
    13801 tttataac
//
LOCUS       DAJSKS010000068        13791 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJSKS010000068 DAJSKS010000000
VERSION     DAJSKS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.069"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..313
                     /locus_tag="ON396_001686"
     CDS             <1..313
                     /locus_tag="ON396_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113773.1"
                     /translation="LMGGMFLSSIDSTTLNTTELTSKDIDMLVNLSGNVVLFGGITLV
                     FSIMIFIIGFLVRKNQKTVVYGISLLILSIIPFFLLTLLWVIPGVLGIIAGLMLLIRK
                     S"
     gene            complement(809..881)
                     /locus_tag="ON396_001687"
     tRNA            complement(809..881)
                     /locus_tag="ON396_001687"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(846..848),aa:Lys,seq:ctt)
     gene            1262..1831
                     /locus_tag="ON396_001688"
     CDS             1262..1831
                     /locus_tag="ON396_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HCR3113774.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1818..2678
                     /locus_tag="ON396_001689"
     CDS             1818..2678
                     /locus_tag="ON396_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR3113775.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2868..3572
                     /locus_tag="ON396_001690"
     CDS             2868..3572
                     /locus_tag="ON396_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3113776.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3577..5412
                     /gene="walK"
                     /locus_tag="ON396_001691"
     CDS             3577..5412
                     /gene="walK"
                     /locus_tag="ON396_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCR3113777.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5409..6725
                     /locus_tag="ON396_001692"
     CDS             5409..6725
                     /locus_tag="ON396_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113778.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6726..7595
                     /locus_tag="ON396_001693"
     CDS             6726..7595
                     /locus_tag="ON396_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCR3113779.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7650..8459
                     /locus_tag="ON396_001694"
     CDS             7650..8459
                     /locus_tag="ON396_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3113780.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8562..9191)
                     /locus_tag="ON396_001695"
     CDS             complement(8562..9191)
                     /locus_tag="ON396_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR3113781.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9361..10653
                     /locus_tag="ON396_001696"
     CDS             9361..10653
                     /locus_tag="ON396_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3113782.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10729..11391)
                     /locus_tag="ON396_001697"
     CDS             complement(10729..11391)
                     /locus_tag="ON396_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR3113783.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11616..11900
                     /gene="groES"
                     /locus_tag="ON396_001698"
     CDS             11616..11900
                     /gene="groES"
                     /locus_tag="ON396_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR3113784.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11951..13576
                     /gene="groL"
                     /locus_tag="ON396_001699"
     CDS             11951..13576
                     /gene="groL"
                     /locus_tag="ON396_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR3113785.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4582 a         2346 c         2943 g         3920 t
ORIGIN      
        1 attgatgggt ggtatgtttc tatcttctat tgattcaact actttaaata ctacagaact
       61 aaccagtaag gatatagata tgttagtcaa tctaagcggg aatgttgttt tatttggagg
      121 aattacccta gttttttcga taatgatttt cataataggt tttttagtaa gaaaaaatca
      181 aaaaacagtg gtctatggta ttagtttact cattttatct attattccat tttttctttt
      241 aacattattg tgggtaattc ctggagtttt aggtattatt gcgggattga tgcttctaat
      301 aagaaaatct taattaggca atgtgtagac aacgtgaaac gattaatcaa ttaaaagcta
      361 aatacgtttt gagaaatgat aacttgtagt cgatggaaga catgtagata aaaaaactac
      421 gtgtctttta tttattctaa ttttttcgaa aaaaagacac tctctgattg ttgtattaat
      481 ggaaaagggg aaagcagcaa gtaaatttag tttttctatt aaatattctt cactttttaa
      541 ccttttatag tacttagatt taatcctata tgtttagaga gttcattggc agcatgattt
      601 tgcaaagctt ctcgaattat tgttatatta gaaactttac tgttcataat tatgcttctt
      661 ttttaactca aagtccaaag ggctacagtg gactttgagt taaaaataaa aagccctagt
      721 tattttgaaa aataaaaaaa gaacagcgta aatgttttcg aaatgctttc gcaccaacat
      781 ttacctgttc cgtgggtttg gcacccaatg ggccgtgagg ggctcgaacc cgcgacccgc
      841 tgattaagag tcagctgctc taccaactga gctaacggcc cgtacaaaaa gtacttaata
      901 atagtagcac taagaatttt aaaatgcaag actacattta aagaaaatca gaaaaatctt
      961 tacttcctta taggaaatca ttcatagatt attcacattt gctcgctatt atataagcat
     1021 accaacaaac aaagttcttg caagagaaca aaccctaaca cttttttcat tttttactcc
     1081 tttcccgccg tctccccggc gggtctcctg ttcttaacga taaaagacaa gaaggcttag
     1141 ccttcttgtt ttttgttttc atggaatccc gctaatcaaa tcacctagtt aaagcaacta
     1201 gattgaatgt ttatcataag tctgatatca tgtttatgaa atgaaaaaag gagcggttat
     1261 gatggaaaaa atgcgtcagc aattaatgga gtgggggaag gaactagaaa cattccgtct
     1321 gcctcactgg gaagaactcc ctgacttaga actttatatg gaccaagtca gaacgttagt
     1381 tgatcgttat ctttcacctg tgatacaagg agaaaagcat ccattgctta cctcttctat
     1441 ggtaaacaat tacgttaaac taggtttgat accagcacca gttaaaaaaa gatataacaa
     1501 agaacacgta gcatttttac ttgcaatcac tacgttgaaa caagtattga ctattcctga
     1561 aatcaaagaa gggatattgt tccaaggaaa aacagtagga attcgtgaag cctataatct
     1621 attttgcgat gaacaagaag cagctgtgtg gatggtcagc caattagcac aaggtaaatc
     1681 tcatccgcaa aaattcgacc aggcaacccc tgtagaatat attgctgtaa gagcggcaac
     1741 tttatcgttt gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa ccgaatattt
     1801 aaaagaggag aaaacaaatg aaaaacaata aaatcgcaat tttagtagat tcaggtacgg
     1861 atgttccaga agaattgata aaaaaatacg agatgtatat gatcccattg aaaattattt
     1921 ataaagatca ggtatataca gataaaattg acattacccc tgaagagatc tatcaacgtt
     1981 tgccagagga aatccctggt acatctttgc cggatggtga gtcgatcaca gaaatctttg
     2041 agcaaatcaa aaaagacggt tatgaaaagg tacttgcggt aacaatttcc agtggattga
     2101 gcgggacata taatgtggtt cgtttaattg cacaacagca agaagaattg gaagcatatg
     2161 tactagatac gaaaaatatt ggtattggtg caggtttttc tgcgatccaa gcagcaaaat
     2221 ggctggaaga aggagtagaa tgggatcaac tgatcagtaa tctgaatgag ttagtcgaaa
     2281 gaacaaaagt tttctttaat gttgcgacat tggaatatct tcaaaaaggt ggacggatcg
     2341 gtttagttgc ttctattgta ggaaccgctt taaagcttaa cccaatcatc tcatgtaatg
     2401 aagaagggat ttattacaca gtaggaaaag ctaggggacg taaaaaaagt ttggataaaa
     2461 cggttgctta cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta gctgttgccc
     2521 atggggatgc gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga gagttcccac
     2581 aagcaaacca aatctacttt ggccagattt ctccggcatt agtagtccac actggtccgg
     2641 gattattggg tgtcggtgta caagttttag aaggataaat caaaaaaagg aaacactgag
     2701 gtgaaaatca gtgttttcct tttttattga agttagaaca aaaaataata aagtattttg
     2761 cactttcatt ttaattttct cttttgtttt ataggcaaag atgaaacaaa agcagaaata
     2821 cggtataatg agaaggtatg acaataatat ataagaggtg gctctttatg aaaaaagttt
     2881 tagttgtcga tgatgagaag ccgatttcgg atatcgtcaa attcaattta gccaaagaag
     2941 gctatgacgt ttatactgca tatgatgggg aagaagcctt ggaaaaagtt gctgaggtag
     3001 aacctgattt gattttactt gatttgatgc tacctaaaat ggacggctta gaagttgctc
     3061 gtgaggtacg caaaacttat gatatgccga tcattatggt aacagccaaa gattctgaaa
     3121 ttgataaagt cctaggctta gagttaggcg ccgatgatta cgtgaccaag ccattttcca
     3181 accgagaatt agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg gctgcgaagg
     3241 aaccagaaga ggcagcacct gcggaattga cgattggtga tttgacgatc catccagaag
     3301 cttacatggt gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa ttcgaactac
     3361 tcttttactt ggcgaagcac cttggacaag tgatgacaag agaacattta ttacaaacgg
     3421 tttggggtta tgattatttt ggagatgtac gaacagtgga tgtaactgta cgtcgtttgc
     3481 gtgaaaagat cgaagataat ccaagccatc caaattattt agtgacacgt cgtggtgtcg
     3541 gttactattt gcgtaaccca gaacaggagt aattggatga aaagaaaagt ccgctttttt
     3601 cgatcagtca attttaagat cgctattacc tttattctga ttttattgat ctcgatcgaa
     3661 attattggtg cctactttat acgtggttta gaacgttcca cgatcaatac atttattaaa
     3721 gatatgaatc aaacggtgga atcattagca acaacaatca gcccggaatt gaatcgaaaa
     3781 gataatgcag atgatgaaga ggtaaatgcg aatatcaaaa gatttattga aaacagtgcg
     3841 actagcgata tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg aggaacaacc
     3901 gatgtcaatg aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat caatgatttc
     3961 acgactaaac gttatgttgc tttagataac gacgataaac gggtgaatat caatgttcaa
     4021 ccgatcctgt ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa aagtaatatc
     4081 gaacaaaaat attcggaaat caataacaca gcagtcattt tctttactgc ctcactaatt
     4141 gcggcattta tttcaatgat cgtatctgtt ttagttgctc gttcgattac acagccgatc
     4201 ggtgaaatga gagaacaagc cattcgaatc gctagaggag actacagtcg gaaagtcaaa
     4261 gtgcatggac aagatgaact tggacagctg gctgatacct tcaatcagtt agctgaacgt
     4321 attgaagaag ctcaagatac aatggaagct gaacgcaatc gtttggatag cgtgctatcc
     4381 catatgacag acggcgtcat tgcgacagat cgcaggggta aagtcattac gatcaatgat
     4441 atggctgttt ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc gattttgaca
     4501 cttttagata tcgaagaaga atatacgttg cggaaattat tagaagatcc aaacgaaatg
     4561 gtacttgacc gttcaaccag tacattggaa agtgatcaga tgatcttgcg agtagacttt
     4621 tctatgatcc gtcgcgaatc aggttttatc agcggattag tagctgtctt gcatgatgtg
     4681 acagaacaag aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt ttcccatgaa
     4741 cttcgtacac cgttgacaag tatgcgtagt tatatcgaag cattgagcga aggtgcgtgg
     4801 aaagacgagg aagtagcacc aaattttttg aaggtaacgc tagatgaaac agaccgaatg
     4861 attcgaatga tcaatgacct cttaaatcta tctcgcatgg atacaggtaa tacccagctt
     4921 caattagaat atgtcaattt caacgagtta gtgaattttg ttcttgaccg gtttgatatg
     4981 atggtgggaa atcaggaaaa aaattataag atccgtcgtg aatttacgca aagagatctt
     5041 tgggtcgaag tagatacaga taaaatcatc caagtagtcg ataatatcat gaataacgcg
     5101 atcaagtatt caccagatgg gggaacgatt acttgtcgct tattagaaac gcataataac
     5161 gtgattttaa gtatcaccga tcaaggattg gggattccta aaaaagatct gaaccgagtg
     5221 ttcgagcggt tctatcgagt agataaagca cgtgcaagag cacaaggtgg gacaggactt
     5281 ggattagcta tttctagaga agtgattaag gcgcatcgag gcgctatttg ggcagagagc
     5341 aaggaaggaa aagggtcgac tttttatatc tcgctgccat atgaacctta tgaggaggaa
     5401 tggtgggaat gagaatctct gaaaaagtcg ttcgtatagg actcattttg atggttgcgt
     5461 taagcatata cttctcttat gcaatctggc tgagcccagc aggaaaaagt tcaatcaacg
     5521 tagatgagac aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact tccgaagtct
     5581 ttcttccgct tcatgttaca tggcaccatt cgggaatgat ccaagaaacc aacagtgaaa
     5641 atctcgtgta ccgattgcaa ttgatcatcg ataaagcaag atttggaaag ctttctgaag
     5701 tagtttcagg agacaaatca cagtttgaac aggtgaaaaa tctgaactct ggttttgagt
     5761 tggcctataa tgcgcctttt ttgctaagag aatacaagga agcatttgat ttagatttag
     5821 atgtgtcgaa cttggcaaaa gaaagcgaaa atatgtattt tactagaata caattcgatc
     5881 aaggaaataa aaaaatccga tttttgaatt attcgaacca tctcgtatat gaagcaaata
     5941 tttcgttgaa ttggacagat atcaataaag aattaacagc aaaagacaca aaatggacag
     6001 aaatgacaac agaaccatcg acgatcgaca cgcaatatgc tacgaaacat tcgatcaaac
     6061 tgaagaagta tagttatatc ctgtcacagc aaccctatac actttttaga aatgcttttt
     6121 tccagaaacc agatagcgtg aaaaataatg atgaaagcaa tgaattgatt ttttacggtg
     6181 ggaatgaaac aatgtctatc cattcggaaa cgcaagtagt tgattttcgt ggagagctgc
     6241 cagagaaaaa agaaaatgct agtgtatttt ctgaaagttt cccctatatt agcaaattag
     6301 gcagttcttt agggaacctc agatattttg accgcagtga caatacgatc gattatcgga
     6361 tattcgtgga aggcttcccg gtttttggga cagacagcag aggacagatt ggtgtggaag
     6421 ttggcggtga aacgaatggt tctgtgcaag tgaatatcca aaccagtttg aatacgattc
     6481 aagtgccgat accttcagac gaagaagtag aacttgcaag tacgaatgaa atcatccagc
     6541 agttaaaagc taagggagca gatagcaaca agattggttc agtgatcgtt ggctatacat
     6601 ggcaaaatgt caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg tatgtgaaat
     6661 ataaagataa ctggtattca gctaacgacc ttctggaaaa attacctgga ttggaggcga
     6721 attaattgga tttcaaaaaa atcgaatgga tatttttcgt cgctttttta gggttgaatg
     6781 tgtttttatt cagcagttat catgacgcgg tataccaaga aaacaatgtc attcgctcta
     6841 atcagactga atcaatccaa caacgtctgg ctagcgacga tatcacttac acggggaaga
     6901 tttcgtctga gaacaagcaa ggctattatc ttagcgcgga gcagaccaat cttagcactt
     6961 cattagcgaa ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt gtgtttttag
     7021 acggtgatgt tttgacgaaa tctttatcca gttcagatgc tgaaaagaac gtgatcgatc
     7081 cagataaaat tagtagttcc ttggaagatt ttttatctaa caaagacaat attttattcg
     7141 gtaatgatta tgtgtacacg aaaaactttt ctcatttaga agaagaacca ctagaaatcc
     7201 aagcaagcca agagtatgaa ggcatcccat tcaatgatga tacagcaaaa atcgttcttt
     7261 ctttagaaaa aaacgataat gattatagca tcacgaaata catgcagacg catttatctg
     7321 atatcgagca gctgcgcgaa aagacggaac tgtatacgga agaggaagca atcaatacat
     7381 tgtacatcaa taacaaaata ccaagaggat caaaaatttt atggcgtcag ctagcttatt
     7441 cctgtattct gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat gtagcgatcg
     7501 aaacaagcga taaagtggtc caaattgaga gcgtcaacgc tttcagcaat acgatcgtga
     7561 caaacaatac tgtaccaaaa gtggaagatc aataaaaaag cgagtataat gaaaaaagtt
     7621 tacaaagtaa gaagggaagt aggagtcaaa tggctgaaga aaaaccagct tttaaaatca
     7681 gtatcctagc aagtggaagt tcgggtaatt cactttatat cgaaagtgaa aaaaaacggc
     7741 ttttggtcga tgcaggactt agcggtaaaa aaatcacttc cttgatggct caaattgatc
     7801 ggacaccgga ccaattagat ggaatacttg tgacgcatga acatcgagat catatacatg
     7861 gcgtgggtgt cttggctcgt aagtatcatt tggatgtttt tgccaatcag cagacttggg
     7921 aagcgatgga accactgatt ggaaatgtac cagtggaaca aaaacatcta tttgagatgg
     7981 ggaaagtcag aacattcggc gatatcgata ttgagagttt cggcgtctcc catgacgcag
     8041 cggctccgca attttatcgt ttccataaag aaaatcggtc attcgtcatg ttgacggaca
     8101 ccggatattg cagtgattat atgcgaggtg tcattgaaaa tgccgatggt tatttgatgg
     8161 aaagtaatca tgatcttgag atgttacgca tgggacctta tccgtggaat ttaaaacagc
     8221 gtattttagg agatagagga catctttcca acgaagacgg tgcacttgtc atgactgatg
     8281 tgatcggtaa tcggaccaaa cgaatctatc ttggccatct tagcaaggaa aacaatatga
     8341 aagaacttgc tcatttaaca atggaaaatg ttttgaaaga aaaagatttt ggcgttggac
     8401 atgattttga aatctatgat acagatccag atgaagcaac tgagctattt tcgatttaaa
     8461 cagttcaatc aataaaaata taaaaagcag aagataaaat caatcagggt tggttttatc
     8521 ttctattttt atttttttag catattaaaa aaataaccgt attatcgact gactacactg
     8581 atgtgctgct tttcatgttt ctcatccagt atctcatcta ctaatgcaat cgcgtaatct
     8641 gccatgctga tcgtactgtc gctagcagcg tttctttgta aataatcatc acttaacaaa
     8701 taagtacctg tacgtttgcc atgtggatca aataaagaag ctggactgat ataagtccaa
     8761 tgtagatttt tagattgttt caattttaac aatccttcgt acatattgaa agctgttgga
     8821 tagaagggtg catttttagg tacttggtca atcatacgag tcgtcttttc tgtatctaag
     8881 aataacgaag aggcaccccc aacgacaatt aagcgtgtat cggttccttc aagcatctca
     8941 atcaaatggg acaaagaagt ttgatgcatt tcttcttttc ctttgggagc gttgaaagca
     9001 tcaacgacaa catcaaaaga tgcaagatca gtagttgtca actcaaataa atccttttgt
     9061 ataaacggaa catctgatgg caatttttct ggatgacgca cgattgccgt cacgtctaaa
     9121 tctctggaca atgcttcgtc taaaataaat gaacctgcat ggccagtagc tccaatgagt
     9181 gcaattttca taaaaaacct ccttttttat tcctgtccat tgtaagcagt tgctgaatat
     9241 tcgtcaaaga atccgatttt tgaaaagctc agtgcgcatt cctttcatgg ttcgtgatca
     9301 ttgggtatag ttaaattaag aaaactgatt gataaaaata gaagttgaag gagataagaa
     9361 atgaaagagt ggttagatcg tgccttttta ccaatgataa catctgttgt gaatcttaga
     9421 cccttaactg ctttgcgtaa tggaatgact tacacgatcc caataattat tgttggctcg
     9481 ctgtttttct tatttgctca ttttcctgtg gattacatcg ctcagtggat agacaaaaca
     9541 ggattttcta tgtattttac acaagtatac caggctacat ttgatgtgat agccttttgg
     9601 atcgtttttg ccattgcata cgcttatgca aaagaagaag acatcgatcg cttaccagtc
     9661 ggcataacag ccttgtctgc ttatctactg cttattcagc cactaaatac tcagatagat
     9721 actgtctgga caggtgctga agggatacta ggagcagtta caactgggct catcatcgga
     9781 tggggctatg cacgtttagt aagacttgcg aaagggtgga agcaaagaga gaagatacct
     9841 gaaaatgttt ggcagtcttt tacttcattg attccaatca ttgttatagt cacttttacg
     9901 cttatcggtt cagcggtttt tcaacatttg ttcagaatga ctgttgttca aggagtatgg
     9961 acatatgtcc agcttcccat tcaatcgttc atagacacgt tgtttggtgt gcttttatta
    10021 ggtttttttg ttccgttttt atggttattt ggtcttcatg gatctgcgat ggtcaatggg
    10081 ttggttagtc ccgtcttgca agcgaattca ttagcaaatg cagaaatctt agcctcagga
    10141 caagagttga cggtggcaaa tggcggacat atcgttactc agcaatttct cgatcagttc
    10201 atgacggtta cgggtgctgg attgacgtta ggggctgtat tttttatgac ggtttttgcg
    10261 aaatcgaaga aatacagaga attaggaaat cttgctcttt taccagcctt tttcaacatc
    10321 aatgaatcaa ttattttttc cacaccgatc gtgatgaacc cgatgatggc tgttcctttt
    10381 attttcgcac cggtcgtttc aggattgatt acctattccg ctttatattt tggattcgtt
    10441 cctttattta cagcagtcca agtcccttgg acaacaccgc cgatcctctc gggtttctta
    10501 gtaggcggag tgcctgcagc gattttacaa ggtatcgtgt tgtcaattag ttttttcatt
    10561 tatttcccat ttttgaaaaa aatagattct gcaaactgca aaaaggaaag acagacaaaa
    10621 aatgatggag cagcagaaaa gatgaacgac taaaaaacaa aaatactcct gtcgctttag
    10681 attgggaaaa tcaccaatct aggatgacag gagtatttat agtcatgttt atactagccc
    10741 tttttggaca gctaattgaa caagaacaac taatgtgttc attcctacgt gtgtgatcat
    10801 cgatgtccag atccgaccag ttgctttgta ctctaggctg aagaagaaac ctaggaaaaa
    10861 gtagattagc agatgaccat cattatgagc aaacgcaaac agcaaagagc tgatcactgc
    10921 tccgacccaa aaatttgaat atcgactgat gatccccagt atactgcggc ggaatacgaa
    10981 ctcttccatg atgggtccag caacagttgt cgccaaaata aaagcgggtg cttccatgat
    11041 catctggatg atattttgtg tattctcgga aggggttgct ttgccaaaca atacagattc
    11101 taacatgaca gcgatgcttt gcagaatcaa tgcgacaaag attccaacca atccataaat
    11161 aacaatcttt ggagcagaag ctttttttac cgctgattcg atcgctaagg gttcacgttg
    11221 cttaaaataa aaaatagcta ggacaacggc gcctagtata tacatgactg cagttgctgt
    11281 gatcaattca gacgtagatt ttacaagtcc tatcgaagct aggatcaagg gtgagataaa
    11341 aattagacca tagcaaaaaa tgctgaggaa gctatatttt tttaaagaca tgtaattcct
    11401 ccttttaagg gataacaagc atgcaaggca agcttatatc gattgatttc aattatatca
    11461 taatttcaat aagcagctgt cagaataaaa tttcttttct ttgatttaaa agcttgcaaa
    11521 aaagagggag aatgagtatt attaaaaatg taggttagca ctcgaaggtt aagagtgcta
    11581 acagattaaa aaaagagtta tcggagggat ttatcgtgtt aaaaccatta ggtgatcgcg
    11641 tcattatcga agtcgcaaaa gaagaagaaa aaactgtcgg aggaatcgtt ttagcttctg
    11701 ctgctaaaga gaaaccccaa actgggacag ttgttgcagt cggtgaaggc cgtttgttag
    11761 aaaacggtga aaaagttcct gccgctgtaa aagcaggcga ccaagttatg ttcgaaaaat
    11821 acgccggtac agaagtaaaa tacgaaggaa aagaatatct gatcgtcgca ggaaaagata
    11881 tcatggcgat tgttgagtaa atttggaaga taaaaaaata atcaaaaata atcaatgagg
    11941 tgaatcaatc atggcaaaag aattgaaatt tgcagaagac gcacgcgcag ctatgttacg
    12001 cggggtagat aaattagcag atacagtgaa agtaacatta ggacctaaag gccgaaatgt
    12061 cgtactagaa aaatcttacg gctctccatt gatcacaaat gatggtgtga cgatcgcaaa
    12121 agaaatcgaa ttagaagatc attttgaaaa catgggtgca aaacttgtat cagaagtagc
    12181 atcaaaaaca aatgatattg caggagacgg tacaacgaca gcaactgttc tgacacaagc
    12241 aatcgttcgc gaaggtttaa aaaacgtaac tgcaggtgct aatcctctag gcattcgtcg
    12301 cgggatcgaa ttggcaacaa aagcagcagt cgaagaattg cataatatct caactgttgt
    12361 tgattcaaaa gaagccattg cacaagtagc agctgtttct tcgggttctg ataaagttgg
    12421 acatttgatt gctgatgcaa tggaaaaagt tggaaacgac ggtgtcatca caatcgaaga
    12481 atcaaaaggg atcgaaacag aactagatgt tgtagaaggt atgcagtttg atcgtggtta
    12541 cttatcacaa tatatggtaa cagataacga caaaatggaa gctgttctag aaaacccata
    12601 tatcttgatc acagacaaaa aaatctctaa catccaagat attctgccat tgttagaaca
    12661 aatcttgcaa caatcacgtc cattgttgat tattgctgac gatgtagatg gtgaagcatt
    12721 gccaactctt gtattgaaca aaatccgtgg aacattcaac gtagtagctg taaaagctcc
    12781 tggtttcggc gatcgtcgta aagcaatgct tgaagatatc gctattttga caggtggtac
    12841 agtaattact gacgatctag gtttagaatt gaaagatgca acaatcgaaa acttaggaaa
    12901 tgcttctaaa gttgttgtag ataaagacaa tacaacgatc gtagaaggtt ctggagaaaa
    12961 agaagcaatc gaagctcgtg tgcaattgat caaaaaccaa attgctgaaa caacttctga
    13021 ttttgaccgc gaaaaattac aagaacgctt agctaaatta gcaggtggtg tagctgttgt
    13081 taaagttggt gcagctactg aaacagaatt gaaagaatta aaattacgca ttgaagatgc
    13141 attgaatgct acacgtgccg cagtagaaga aggaatggtc tcaggtggtg gtacagctct
    13201 agtgaacgtt atcagcaaag tttctgctgt agaagcagaa ggagacgtgg caacaggtat
    13261 caagatcgtt gttcgtgctt tagaagaacc aattcgtcaa atcgctgaaa acgctggtta
    13321 tgaaggatca gtgattgttg ataaattgaa gaacgtggaa ctaggcactg gtttcaatgc
    13381 tgctactgga gaatgggtaa acatggttga agcaggtatc gtagacccaa ctaaagtaac
    13441 acgttctgcc ttgcaaaatg ccgcttccgt ttctgccttg ttattaacga cagaagcagt
    13501 cgttgcagat aaaccagaac cagcagcgcc agcagctcct gcaatggatc catcaatgat
    13561 gggcggtatg atgtaagaaa aagcaacttg cttatctaca actgatcatt agtgaaagac
    13621 agataaaaag aggctggaac agaagcgttt aactccaaga aataagaaga aattcacgga
    13681 aattgctttt caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc
    13741 gctgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt c
//
LOCUS       DAJSKS010000069        13635 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJSKS010000069 DAJSKS010000000
VERSION     DAJSKS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.070"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            34..1461
                     /locus_tag="ON396_001700"
     CDS             34..1461
                     /locus_tag="ON396_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3113786.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1553..1918)
                     /locus_tag="ON396_001701"
     CDS             complement(1553..1918)
                     /locus_tag="ON396_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCR3113787.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1915..2280)
                     /locus_tag="ON396_001702"
     CDS             complement(1915..2280)
                     /locus_tag="ON396_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCR3113788.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2472..2720)
                     /locus_tag="ON396_001703"
     CDS             complement(2472..2720)
                     /locus_tag="ON396_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113789.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2719..2847
                     /locus_tag="ON396_001704"
     CDS             2719..2847
                     /locus_tag="ON396_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HCR3113790.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2904..4598
                     /locus_tag="ON396_001705"
     CDS             2904..4598
                     /locus_tag="ON396_001705"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCR3113791.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4602..4721
                     /locus_tag="ON396_001706"
                     /pseudo
     CDS             4602..4721
                     /locus_tag="ON396_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4698..4994
                     /locus_tag="ON396_001707"
     CDS             4698..4994
                     /locus_tag="ON396_001707"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113792.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRTPKLKLLLMFLTWLDLLPQMQA
                     FEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5372..6505
                     /locus_tag="ON396_001708"
     CDS             5372..6505
                     /locus_tag="ON396_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR3113793.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6536..6856
                     /locus_tag="ON396_001709"
     CDS             6536..6856
                     /locus_tag="ON396_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HCR3113794.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6885..7349)
                     /locus_tag="ON396_001710"
     CDS             complement(6885..7349)
                     /locus_tag="ON396_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3113795.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7480..8733)
                     /locus_tag="ON396_001711"
     CDS             complement(7480..8733)
                     /locus_tag="ON396_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3113796.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8762..9079)
                     /locus_tag="ON396_001712"
     CDS             complement(8762..9079)
                     /locus_tag="ON396_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR3113797.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9105..9425)
                     /locus_tag="ON396_001713"
     CDS             complement(9105..9425)
                     /locus_tag="ON396_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3113798.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9889..11304)
                     /locus_tag="ON396_001714"
     CDS             complement(9889..11304)
                     /locus_tag="ON396_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3113799.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11321..12637)
                     /locus_tag="ON396_001715"
     CDS             complement(11321..12637)
                     /locus_tag="ON396_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3113800.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12787..13485)
                     /locus_tag="ON396_001716"
     CDS             complement(12787..13485)
                     /locus_tag="ON396_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR3113801.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         4325 a         2773 c         2351 g         4186 t
ORIGIN      
        1 ttgtgcaaaa aaatacaagg cggaggctct actatgattg aagaatatat tgagaaaaat
       61 attttaagac agctgttttt atgtgggcaa ttctatgtaa ataaagaagt caatctagaa
      121 aagctctcca atctcctgca tgtttgcaaa accactttac tcaatgatat caataacata
      181 aaaaaagaat ttgaagaaca gatcgcttat acacatcggg aaaaggaccg ttacacttta
      241 tatttttcag aacatatacc tcgatgcaag atcatgcagc aattgtctca aaattcgttg
      301 tttttaaaaa cttgtctgct ttatttggaa gaagatgaac ctgattatct tcaattaact
      361 gaatgtgaat tcatctctgt cagcaaagct tattcattaa aaaaacaagt attagcttat
      421 tttaatgact gcgggatcga aatcgaccgc tactcccctc gattcactga aatggaaaga
      481 cgtctgctgc ttcttaatgt gtcgtatcgt ttaggcggtt ttaattcttg ggagctacct
      541 gaatcgtttt ttgaacgtgc cgatcgtttc attgaatccg taacggaaaa tagtggcaga
      601 ttttatgata aagaaaataa agaaatctta tcgattggtt ttgctatcag tttcctacgc
      661 cagcaagtgt gtgctgtgac aatagattca aaatttatcg aagaaatcaa aaaacgtcct
      721 atttacaatt atgtcgaatc agcatgggaa aacactgatt tccaaaccta ttataaaaaa
      781 gaagaattcg cctttatttt gacactattc aatctttgta attatggttt ccattcttat
      841 cagttgattg cggaagactt tcaacagcta caccaagtgt ttattgacaa tactcccgaa
      901 ataaaagaac tagttgctac ctttgaatct cacttcaatc aagaattatt cggaaaccaa
      961 ccgttcgaac gtgcgctgat tcatctgatg cgttcggctt gggataacta tcaattattc
     1021 atgccagaaa aattttatct tttaaatgag gaacagacaa atttatataa agaagtccag
     1081 acaatttttt ccagctggtc cagccagtta ccttatgatc tacggttgaa tcctaattgc
     1141 atgcgggctt ttgtgatcga attgagcgga attctccgtc tgacaaaaga acatctgact
     1201 atctatatcg tcacaaattc ggatgttcat tatctgattt atcgggaagc cttggaagct
     1261 gtaactactt tcgattttca agttgcacca acaatttatt caagtatatc tgatataaag
     1321 aagtatgcgc agcagtcctc aaaccgggta ttatgtgaac gtacattata tacaccagat
     1381 gcggtccaat atgaaaatat tattcctatt tcgatcaata cgatcgatcg agctatcatt
     1441 tcagctgtgc aaaacaaata aggggctgtg acaaaaatca tgtcacagcc tcttatttgt
     1501 tgaagatgcc catttctgta acaatctctt gatacacgtc aggctcacaa cctcacttat
     1561 tttggacatc tacgggtccg atctctttat ttaaaaaata agaaatgatg gttcggataa
     1621 ctaccaacgc cgctagttgg aaaatatcct gcaaggtagg tttgataatc gattcgatga
     1681 tcccagctga aacaagaatt tccaaactta gaagtatata gcctcccaac atctttttaa
     1741 tagcattgtt ctcgctgact cggtcgcttt gcacttttgt atggacaaat aaatatttca
     1801 aaaaatcctt gattgataga caaacacccc aaaccaataa acagatagca aagatatcaa
     1861 gtcccagtac aagtttttcg aaatatggaa gaagaaactg catcaactct gtcgtcatcc
     1921 tacctctcct cttctttgtt tttctttgtc ttctatttcc tgattcaaga aataagaaat
     1981 gactgttcga atgataataa taagtgcaag cttcaaaata tctgtccaag tcggcttgat
     2041 aactgactcg atgatatcag caacgactag tacttccaag cttaacaaaa tataacttgc
     2101 tagcagtttt cttgtatctg cattacgagt attacgttct gcatagtttt tcttgacatg
     2161 agaccaatca aaaagtttga atagaacaag aacgatcccg acaattaaga taccgataga
     2221 acaaatattt aaaatagcaa tgatccattc tagaaattca attaaatttg agcgaaacat
     2281 atacgctacc tactttcttt taatttgttc ctattctttt attgtaaaag aaagttacct
     2341 tgttcgtcta tttaaaagtt gttctaatga taacggttcg cttaaatttt tctttttcac
     2401 tctcccattt ttctccctcc atccataaat cgtggaagta tccaaaacaa cgtagcaagt
     2461 taaaataagg cttagtccgt catcagcttt tcaaagaagc actgtctcgg tgcttttttt
     2521 gtacccaaat ttctaaaaat agaaccatac acctcctcaa atcttttctt cttacctacc
     2581 ctatttttat ttacaggaaa atcagacact tcttcttttt tgtcaaaatt tcctttttct
     2641 tctcgtccta tcatttggca ttctaataaa aacgaactat ccttggatat agaaagagaa
     2701 aacaaggagg cgtttttcat gagtaaaaaa ttaatcaaag tcggcatcgg tttaggtttg
     2761 cttgcccttg gcgctgcata tctaggaaaa aagacaggac tattcgaaga tgacagtcat
     2821 ttatatgatg aattcgaatc aatctagcat cctatttagt tgaatcacta cggacatact
     2881 tggaagacat tggaggaatc acaatgagaa aaagtaaagc aataatgatt ggtgccggta
     2941 ttgccaatat ggcagcggct gtttatttga ttcaagaagg caaatggaaa ggggatcaga
     3001 tcacctttta ttcattggat gatcacggtt caaatgatgg tgcaccgacc gacactgtag
     3061 tggatgaata ttggaataaa aaccatccat tagaaaacca aaaaggctat attgctcgag
     3121 gcggtcgcat gcttaattac cgtacctatg tagatttgat ggatctgtta agtcgaatac
     3181 cttctgttac tgagccaggg atgactgctg aagaagatac gagagatttt gatgcaaaac
     3241 accgaacctt tgacaaagca cgtttgatgg aaggtggcaa aggaatcatt gatggcggaa
     3301 aactgggatt caataataaa gaccgtattc ttctgactaa actgattgct atgcctgatt
     3361 cagaagaaga aaaactagac aatatcacta tcgaagaata tttcaaagat gatccacact
     3421 tttttgaaac aaacttctgg tttatgtggg aaacaacatt tgctttcaga acacgtagtt
     3481 ctgctcaaga attgcgtcgc tatatgcatc aaatgattta tgaattcact caaatcgaac
     3541 atctagtggg cgtcaatcga acacgttaca atcaatacga aagtattatg cttcctttga
     3601 tcgagtactt gaaagaacaa ggatgcaaaa tcattttgaa ccgccgtgtc actgactggg
     3661 aattcaaaga aacaccgatg caagatgaaa tcactgtgac tggtttgaaa attaccaata
     3721 cactgactca agaagaagaa tacgttgtgg tcgatgaaga tacagcagtt attttcacta
     3781 atgggtccat tacagattca gctactttag gagattacga aacaccagct gtagaaaata
     3841 tggattatgg tgcagcttct agtctctgga aaaaagccag tgaacgtttc tacaacttgg
     3901 gaaatcctga caaattcttt gctgatcgag atgcaagcga atgggtcagc tttaccctga
     3961 cgacgaaaaa ccatttgttg ttgaatgaaa tcacccgtat cacgactcaa gaaccaggaa
     4021 atgccttgaa ttcatttatt tcaaccacac cgatcacgcc gttaggccaa aaagatgtca
     4081 atatgtctat cgtcgtgcat catcaaccgc attttacgtc tcagaaacca aatgaaacag
     4141 ttatttgggg ttatttcctt tatccaagac gacgtggcga atttgtggat aaacagtata
     4201 ttaaaatgaa tggaaaagaa atgctagaag aactgattgg acaactttca aaagtagatc
     4261 ctggaccagt gaatattcgt gaaaaagaaa cagaaatttt cgacagcgta ataaataata
     4321 tccctgtcta tatgccttat gcttccgcgt tgttcaataa tcgtgccaaa agcgatcgac
     4381 caaaagtcat tcctaaacat tcgaccaatc tggcctttac aggagaattt gtcgagcagc
     4441 cttatcagat gatctttact gaacaaagtg ctgtacgttc tggagaaatc gctgctttcc
     4501 attttgcggg tattcctatg tcccgtcttg tcaaaacacc gcgttacgac aaagatattc
     4561 ctactctagc tcgtgccgca aaaaaaatgt ttgaataatc aagaggttgt gaaaaagctg
     4621 ccctgcatca agtaataaga acaaccaggc taaaacagct tcttatgttt ttgatttggt
     4681 tggacttatt accgcagatg caagcttttg aacaccgttt accaagaggt tgtgaaaaag
     4741 ctgccctgca tcaagtaata agaacaccca agctaaaact gcttcttatg tttttgactt
     4801 ggttggactt attaccgcag atgcaagctt ttgaacacca tttaccaaga ggttatgaaa
     4861 aagctgccct gcatcaagta ataagagcag ccaagctaaa acagcttctt atgtttttga
     4921 cttggttaga cttattaccg cagatgcaag cttttgaaca ccgtttacca agaggttatg
     4981 aaaaaggcgt ttagtttcga gtatgaagaa aaattttgtg aaaatggttc ctcacgtttt
     5041 tgcataaatt tgacttaata ccgagaaact gatcccttct gtcataacct ctttttattt
     5101 atgtctgttt ctttttgaaa acaactattt tttcaacgtt tactctcttg ccaatccaac
     5161 atcatctaac ggatacaact atttcaatga ttaccattac actaaaaaat cggaaacaca
     5221 cctctccgaa cacaaataca taagactata gaatgtttca tttaagaaaa aaagcgtttc
     5281 attatcaaaa aggtaagatc tcctttaaaa tgaaattatc tgtagtacta ataatgcaga
     5341 caattttatg aatctgaaag gagcatctcc aatgaaagca gtcacttggc aaggaaaaca
     5401 gaaaatggaa atcagaaacg tagatgatcc aaaaatatta gaaccaacag atgcaatcat
     5461 ccgtatcaca gcaacagcca tttgcggctc cgatctgcat ctttaccatc atggtgaagc
     5521 agttttggaa aaagggtatg ttgtaggaca cgaaccaatg ggaatcgttg aagaagtggg
     5581 atctcaagtt caaacattga aaaaagggca acgtgtggtt atccctttta atattagttg
     5641 tggtcattgt catttctgcc tgaatcacat ggaaagccaa tgtgatgaat caaatccaga
     5701 tcagttatat ggcgggcttt tcggatttgg caagttgaac ggtaatcatt ggggtggtca
     5761 agcggaatat ctaagagttc cttatgcaga ctctacttca tttgttgtac cagataatga
     5821 tcttcctgat gaaaaagttt tatttctatc agatattttg cccacagctt attggagcgt
     5881 tgaaaacgca ggagtaaaaa aaggcgatac agttgtgatt ctaggttctg gtccagtcgg
     5941 tctattcgct caaaaattcg ctgttatggc tggagcagag cgcgtaatcg cagtagatcc
     6001 agtacagcac cgtctagata aagcggctaa ttacaatggg gttgaaactt atttcttaga
     6061 agacacggca caagctggaa cagaattgta tgaactggct aaaggtggcg cagatgtcat
     6121 tatcgattgt gtaggaatgg acggattaga gcctgtcaaa gaaaaagcga aaaatttagt
     6181 aagcttacaa agcggcacaa tctctccatt acagatggca tcgcaagctg tcaaaaaatt
     6241 tggaacagtc caagtaactg gtgtttatat gactccagct tcttcttacc cgctgcaaga
     6301 attttttatg cgggatatcg ctgtcaagca tggacaggca ccaattatcc atctaatgcc
     6361 aaaaatttac gcaatgattg cagaaggact attcgatcct acacaaatca ttactcattc
     6421 gctgccacta tcagaagcag cacaagcata tgagatcttt gacaaaaaag aagataaaaa
     6481 tataaaagtt gttttgaaac cttgaattca gtaaaaaata caggtgattt tctttatgcc
     6541 caaaatcaaa cgaaataccc atgagatcga aataggaaaa gaagaagata tcgaaatacg
     6601 aggacgtcgc ttccgcttct atttccaaaa tcgttacaca ttgatctcac tggctgtcga
     6661 tttattaacc gggattttct atatttttgg aagccttgct tccttaacta aaatccccga
     6721 tcgatatggc acctattttt atttggctgg tgctattttc ttgacgattc gtcctattct
     6781 cagaatcgta aggaacattt ttatttacga cgaaaaaaaa gcaatgaaga gaacaagcaa
     6841 aaaaaaagag gcatgagaat ttttcaaatc tcatgcctct tttttcaccc tatcgtatca
     6901 ctttgataca gacgagcggt cgtcccactt tcttccttga ccattgtcaa aaatgtccac
     6961 tcgtatcgtt cataaaacgt ggtatgatct gtaattaagt aaagtttttc gtacccaagt
     7021 tgatgcattt cttttctgat ttccgctaat atccgcttgg ctatcccctg cttacgcttt
     7081 tccggttcaa caaacagggc acaaagattt ggtgtcagat cagggcggtt atggaagtcg
     7141 ttttcaatga cgcccgctcc tccgacgatg gtttcttttt catctaagac cacataccat
     7201 tgaggaatac tttccttatt agcgattcca gtacgaatac tttcctcata cacaacagca
     7261 ggaactcccc attttgatga aaaccaattc gcgctttgtt ttactaattc tgggtgttct
     7321 cttaatttga tccagttata tgtaaccaat gtttaaacct ccgactaaaa taaaacgttt
     7381 acaaaaatta tacaagtctt tatgaaaatt agcaatgaca acaatagtga tgtaaaaaaa
     7441 aaacggaaaa caatcacttg ttctccgttt tttgattaat caagcatcta aagcttcttt
     7501 tcttctttct tggatcgtag ctaccttgac gaacggtaga tagatcaaaa tagaaagaac
     7561 caaattgaca gcggctagta caccacctgc aatacttttt gttgccaata caccgccaat
     7621 aattggtgga gttacccatg gaggcataac ggtagctgct ggcactaaac ctgtagaagt
     7681 cgcaaagtaa gcaacactaa ctaagaccat tggtgtcaaa ataaatggaa tgaacatgat
     7741 cgggttcaac acgataggta acccaaacat cgttggttca ttaatattga agacacctgg
     7801 cgcaatcgac aagtttgtta caaccatata tggtttgttt ttacgtccta ctaaataaat
     7861 agctaacagt aaacctaaag ttgcacctgt tcctcctaag ttgacaaagc tgtcaaagaa
     7921 aggtttgttc acgataaacg gtgcgctatg cccagctgcg attgcattaa cgtttgcttc
     7981 aatggctggc gcattgattg tttgcatcaa aggatcgacc atattcgctc cgtgtaaacc
     8041 aaagaaccat aagaatggtg tgataaatgc aagcagtaag gcagatggat aagagtttgc
     8101 aagtcccata aatggttctt ggaccactgt atagaaagaa cctacgatat catcaacacc
     8161 gaatgcagca aaaattgcag caactaaagc aaacgcagag atagtgatca ttgctggtag
     8221 taatgaagca aatgcttttg caactgccgg tggcacacca tcaggcattt tgatgatcaa
     8281 tttttcattt ccgcttaaac gtacaaagat ttctgttgaa ataatcccaa caataatagc
     8341 aataaacaaa ccgtttgagc tcatacctaa tgctccgcct aaagcgaaga aagagcctaa
     8401 agaaatcaca cctgcggaaa caccgtcttt tcggtagccg ttagctaaat gataagcaag
     8461 caagaaagca atcaagacag aaagaatggc aaatgtaccg ttccaagcag cagccccgaa
     8521 tcctttccat gattcaccac caaaaattga gttcatcaat tcttggaacc cgggaatcgg
     8581 taagttattg atcattactg ctaatgctcc taagatcatt aacggcatac tcaccataaa
     8641 tgagtcccga atcgcaacta aatgccgttg tgcaccaatt tttgatgcta caggtataaa
     8701 atgtttctcc ataaatgcaa caaatttatc cataaatctt ccctcttttc tttactgtag
     8761 attacgcgtt caatcgacga tacagatcaa tgatctcttt tgctaaatct gtaaaagcta
     8821 tagatgtcat cagatgatct tgactgtgga ccgtcaaaag cgtgacttca acgtgatttc
     8881 cttgtgcttc ttgtgtcagc atctctgttt gagagtgatg tgcttggact aaagattcct
     8941 ctgcatcttt gattttttca tctgctaaag tgaaatcacc ttttttcgca gcagcaatcg
     9001 cttccattgc atcgcttttt gcatttccgc caaacatgat cagacccatg atggcttcta
     9061 gatttttttg atcttccatt tattgtatcc tccgataatt tttattattt gtttattagt
     9121 ttttctgctt ggtcgagtac tttttcacca ttcatcatgc catagtcagc catattgatt
     9181 acatctaaag gaatgccttt aggtgctagc ttttcagcaa attgattttg cataaagcgt
     9241 acttgcggtc ctaaaagtaa aacgtctaca tttttagatt ctagttgatt atccgcatct
     9301 gaagcagata cagcaaagat gtctgcttcc attccttttg cttctgctgc cttttgcatt
     9361 tttgtgacta ggagacttgt actcattcca gctgaacata caagcatgat tgttttcttt
     9421 tccattttaa acacgttcct ttctatactt acacttgtat ataagcaagt agcgtgccaa
     9481 ctttaaaagc gctttaaaaa gttgatatca tcacatttaa ggtctatacc aagttgcata
     9541 cacactaaaa aagaaagcgc acactaaaca cacaaattaa tttttattac cttttttctt
     9601 ttaacgtgca caaaaaaagt tgtgacacaa aacttgtcac aacttttttc aaataaacgg
     9661 tattcaaaag cttgcatctg cggtaataag tccaaccaag ttaaaaacat aagatgttgt
     9721 ttttactttg ctattcttat tacttgatgc aggacagctt tttcacaacc tctcaataaa
     9781 cggtgttcaa tcagctgacc ctgggtatta gctcaaaacg ctggattcac aacctctttt
     9841 atttcaaata accagtaaat acaatgaaca tctacaaaaa acttctattt atttatagat
     9901 ttcgtcaggc aaatccaatt ctctcttatc aatcaaagtc tgataccacg aagcagattt
     9961 tttcaatgtt ctgcttcgtt catcgttcaa ttcgattcga actaatccat atcgattctt
    10021 aaatgcattc attggtgaca cacagtcagt gaatgcccat aacatatagc cttcgcaatt
    10081 tgcgccttct tctgttgctt tgatcaagta atgcaaatgc tgtccgataa attcgatacg
    10141 gtaatcatct tgcacgattc ctttctcatc catatactgt tcttcatatt ctcttcccat
    10201 accgttttct gttatcagcc aagggatatt atgataattt tcttttagat acatggctat
    10261 atcatacatg attttcggat agatttccca tcccctggaa ttattcattt tcttcccagg
    10321 taaatcaaag acttcataat atcgttcagg atggaaaggt gtctcttcat tccattgata
    10381 acgaggggcg cgaacgcgtt taggataata ttgattgatt ccaaggaaat ctactgtatg
    10441 atgctgaatc gtttctaaat cctctgttgc aggatcgaaa taaatcgatt gtttttgaca
    10501 caactccagg aactcttctg tgtagtatcc ttttaccatc ggatcaaaaa atactcgatt
    10561 ataaaacaaa tcatacatat aagcagcttt ttggtcttct ggagaattgg atcgagcata
    10621 gaccatttca ggatttaaaa tacagccaat ccttccacca taatttccgc tgcggtaaat
    10681 ctctactgct ttagccgttg ctaaaacttt atgataattc cacatcatcc acttttttgt
    10741 gttttgttca tgcggccagc gaacagcatc caaatagcaa cgcgtttggg gaacgattgg
    10801 ttcgttgaat gtaaaccagt tttttaccct atcaccgtaa cgatcaaagg caatttgagc
    10861 atacttagca taaaaatcaa ctacttttct tgaagaccag ccatcatatt tttccaaaag
    10921 aaaagctggg acttcataat gttccaaaca aatcatcggt tctacgtctg cttcgatcaa
    10981 agagtcgatc atttcactta tatgctgagc atattcttca tctactatac agttctcata
    11041 atcagtaaaa aaacgagccc aattgataga agtccgataa tgtgtcagat ggatggattt
    11101 catcagatcc acatcctgct ggtattgttc cataaaatta gttgctacgg ctgggccata
    11161 cccattatgc caaacaaaat gttcattttt ataccagcta tctataaacg aatcctgtcc
    11221 ttcttttttt cctttccaac cttccgtttg ccaagcagaa gatgcagcac caagaataaa
    11281 attttttgga atagtaattt tcatgatttc ctcctgtttt ttagatccca tcattttcca
    11341 cacgatttgc tacttttaca aatggcagat agatcaaaat agaaacaata atacaaacaa
    11401 gttgcgtaat aactgcaccg atatttcctg ctgttgccag ataagcattg acgatcggcg
    11461 gcattggcca agggaccatt acaactgctt tcgccgcaaa tcctaaagcg gttgcaaaat
    11521 aaccaatcgt tccagtgata agaggcgtta agataaacgg aatagctaaa attggattca
    11581 gcatgatcgg cataccaaaa ataactggtt cattgatgtt gaagatccca ggagcaaggg
    11641 ataacttggt gatttgtttc atatcttccc gttttccaac taacaatact gcaatcagta
    11701 aaccgatcgt gactccagac ccgcccatac tcatatacat atcccaaaat ggcatagtaa
    11761 taatgttcgg tacttctttt ccagcttcaa aagcttccgt atttacagca attgccgcta
    11821 aaagcaacgg ttcccgtatc ggcttcacca tttgatttcc atggatacca atcacccaaa
    11881 agacttgcgc aataaacatc aatagcaaaa tacccggtaa cccttgtacg acaccttcta
    11941 atgggcgttg tacgacattg taaatgatat cataaaggta cattcctgta agttctttga
    12001 tgataaatcc cgcagccgcg atacccgata cagtcagaat cgtcgggaat aaagcagaaa
    12061 aactagcaga aacattcgtt ggcacggtat caggcattct cagctgtagt ttcttctgtt
    12121 tcctcaacca gcaataaagt tcaatagaaa ggatggagac caacattccc aaaaataacc
    12181 ctttcgtatc tgtatattgt ttagccaata cgttttctac tataacagtc tgattttcat
    12241 tcactaattc ataaacagtt ggattaacag ataaataaga catcactgcg atgataccag
    12301 ggaatacacc atccacttta ttcagcttgg ctaattcgat ccctatcaga aaaacagcat
    12361 agatggtcaa gaaacttagt gtgacatagc tgatggcaga tgcgataggc tttaattctt
    12421 ctaaaaaacg caagaaactt atctgtgcta agccattttc cgagtcaaag accattgctg
    12481 aaaacaatgt gccaaaagca cccgtaataa ttaccggaat caatgcagca aatgcgtttt
    12541 ttatcaccat gatatacctt aaattattga ttctcgttgc cacgttccct aaaacattgg
    12601 ctaatttatc cataaaactc attccagatc ccctcattcc tgcttcttct ccttgacgtc
    12661 atctcttttt gtctattagt cagcgcttac atttcgatga acagaaacta tttactcctc
    12721 ttttttacca aatataaaac aaaaattcat ctgatgtatt tatcataaaa tacatcagat
    12781 gaactgtcaa ttagtttctt tttctcctat tattttctgt aattctctcc gatttattgc
    12841 catatggaca gtcattgcat caaacgcagc aagtgcattc cgcctttcga tggcctgaac
    12901 gatctctgta tgaatatcga tcgtctcgat tttcagatgc tggatatcat aatttttatt
    12961 aaacaaagca attgattcat taatgattgg gataatatgc agcaaggcta cgtttccgct
    13021 agcttctgca atcatcgtat gaaaatcgat gtctaattga acatgttctt gtgtgccttc
    13081 tcgaaaagaa gtttcaatct tttgcgttaa ttttttcatt tggacgattt gattcttcgt
    13141 tgcatattcc gctgccaaac ctgccatctt aggctccaat aaatatctca gttcaaataa
    13201 gtctcgtatc atccgatcct gatcttcaat caaagaaaaa ccgagtgggt cattggcaat
    13261 ccctcgctta tcactgacaa atgtccccgc tccttgccga acttcaagga tattcctgga
    13321 agctagagcc cgcacagctt ctcgcaccgt gcttcgcccc acctctaaat aatcggacaa
    13381 ttcatattca ttcggcagtt tatctcctac ttgcaactgc atttccttta aatatctaag
    13441 gatcttgtca gtagttactt ctactagggt tttattttct tccaaaccac atacctcctt
    13501 ttcatctgat gtattttttc gtaattatta taacacgttt gaatcatctg atgatttttt
    13561 gtatacaaaa ttgattaatc cgttcataga catacaaacg aagaagatat ttaccataaa
    13621 aaagactagg ataaa
//
LOCUS       DAJSKS010000070        13428 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJSKS010000070 DAJSKS010000000
VERSION     DAJSKS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.071"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..242)
                     /locus_tag="ON396_001717"
     CDS             complement(<1..242)
                     /locus_tag="ON396_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3113802.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQA"
     gene            complement(721..1440)
                     /locus_tag="ON396_001718"
     CDS             complement(721..1440)
                     /locus_tag="ON396_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCR3113803.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1489..3135)
                     /locus_tag="ON396_001719"
     CDS             complement(1489..3135)
                     /locus_tag="ON396_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3113804.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3314..3904
                     /locus_tag="ON396_001720"
     CDS             3314..3904
                     /locus_tag="ON396_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3113805.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3996..5501
                     /locus_tag="ON396_001721"
     CDS             3996..5501
                     /locus_tag="ON396_001721"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCR3113806.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5585..6937)
                     /gene="celB"
                     /locus_tag="ON396_001722"
     CDS             complement(5585..6937)
                     /gene="celB"
                     /locus_tag="ON396_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCR3113807.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6937..7455)
                     /locus_tag="ON396_001723"
     CDS             complement(6937..7455)
                     /locus_tag="ON396_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113808.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7471..7797)
                     /locus_tag="ON396_001724"
     CDS             complement(7471..7797)
                     /locus_tag="ON396_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HCR3113809.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7810..9789)
                     /locus_tag="ON396_001725"
     CDS             complement(7810..9789)
                     /locus_tag="ON396_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR3113810.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9810..10124)
                     /locus_tag="ON396_001726"
     CDS             complement(9810..10124)
                     /locus_tag="ON396_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HCR3113811.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10292..10585
                     /locus_tag="ON396_001727"
     CDS             10292..10585
                     /locus_tag="ON396_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR3113812.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10663..11775)
                     /locus_tag="ON396_001728"
     CDS             complement(10663..11775)
                     /locus_tag="ON396_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3113813.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            12162..12341
                     /locus_tag="ON396_001729"
     CDS             12162..12341
                     /locus_tag="ON396_001729"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113814.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12535..12705
                     /locus_tag="ON396_001730"
     CDS             12535..12705
                     /locus_tag="ON396_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3113815.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(13129..>13428)
                     /locus_tag="ON396_001731"
     CDS             complement(13129..>13428)
                     /locus_tag="ON396_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3113816.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4257 a         2698 c         2284 g         4189 t
ORIGIN      
        1 aatgcttgat ccgaagaaaa taaaaatttg aaattttccc aacccaccca aggactttcc
       61 ctcaagctag ccaagaaccc atctgctgaa atatgaaaat ttttgaatgc tacgacattc
      121 gccattacag gtatgtaaaa gaaaaaaatc atccaaagca tacccggtac agccatcagg
      181 acaagtgctt tataacgcca tagattcata aaaaaatgat tgaacccctt tcgttttttc
      241 atctaaaaac ccctttcgat tcattcgcaa cgtaattata gcgctttcaa taatgagaat
      301 aaataaacaa agtataggga aaatgaaaca aagtatagga gaggttgtga aatcagcgtt
      361 ttggcctgag tattagagaa aaaaacggga aaatagttcc tcatattttg ccgttttttg
      421 agctaatacc gagggtcagc tgattgaaca ccgtttatca gaggttgtga attcagcgtt
      481 ttggcctgag tattagggag atcgtgtcct tactatctaa ctctgagtca caaggaacaa
      541 tggggactgt aacaaaagtc ttgtcacagt cccttattcc gaacaaacgg tgttccagaa
      601 gtagcttcta cggaaataag ccgaaatttc acaaaaaagc catccgaaag tcagacggac
      661 aagtctgctt ttcagatggc gctacaacta ctctgcttat tcttttagct atccattcaa
      721 ttatttttct ctcacttgtt ctagctcttc tttggtcatg ggtcgatatt gccctttatt
      781 caaatcttta ggcagccaaa attttcccat acggattctt tttaaatata agacttcttt
      841 tcctactgct ttgaccattc gcttaacttg gtgaaacttt ccttcatgca aaatcagacg
      901 gataattgaa gtttctgtct cttgatctac ttgatcaatt tggagttttg ccggcaatgt
      961 ctgctcatcg ccatcaataa tcagtccttc tgcaaataaa cggatatctt cttctgtcat
     1021 tataccgcta acctcagcta gatattcttt ctccacatgt tttttaggtg atagtaactg
     1081 atgggcaaac accccgtcat tcgttaaaat caagaggccc tctgtgtctt tatctaatcg
     1141 gccgactgga aaaagatctt cccgataatc ctggtcttcc aataaatcga ttacggtttg
     1201 atcatgatta tcctgagtag cagaaatgac tcctgcaggt ttatgcaaaa tgtagtaaaa
     1261 atctttttga tacgtaatga tttctcctaa ataagtaacc gcatcttcga attcatttac
     1321 ttgcgcttta tcgcttttca ccacttgtcc attgacttcg atcgttcctt ttttgatcaa
     1381 tcctttgact tctttgcggc tgccaagtcc catatctgct aaaaacttat ctaaacgcat
     1441 gcgtttcccc catttcaaaa gagcatcaat ctagctcttc tgattcactt attttatttt
     1501 taatttatgt cgtaaacgcg ctattcctgg tcctagtagt ctttctgcca agcggatttt
     1561 caaagctaaa taaccataca ctaaagcccc tatacctgct actacaagaa tcaacaatag
     1621 agactggaat ttgctatctt cgtttaaaaa agcaccaaaa atcatctttg tgatccacga
     1681 agccaaaagc ataatcaagg taagaataaa gatcagcaat gtcctgcgcc agacaaaact
     1741 tggtttaaag cgggctactt catggattct ctttaagatc aaggtacaag acacagcaaa
     1801 tccgatcatt gtcgccaata aagggccgta tacttcaaat aaacgaatgg ctgggtattg
     1861 aagaaggagt ttcactagaa acccaataac caaataatta attgccgcct tgttttcaaa
     1921 cattccttgc agcatgttgg aaactagcat atatagtcct aggaagagtc ctacaaaact
     1981 tgcttggatc agtacattac ttcctagaga atccggtgta taaaataatg tatttagcgg
     2041 ataggcaagt aacataacac caaatgtcgc cggaaacata aaaaatgaaa acaactgtaa
     2101 atttccgctt gtcaattttg ccagacctac tcgatctttc aacgtaacag cttctgtaat
     2161 caacggtaac ccagtagacg cgatcgatgt tgccaatgca ataactacca tcgtcaactt
     2221 atctggattg gcactaaaaa tcgaaaaaag atccagtaac tgtgcattgg aatactcggt
     2281 ggatgcagac atgatcttca taaaagtgaa ctgatcgact aatttgaaga tcgttacccc
     2341 tgaacccaca ataataaacg gaatcgcttc tttaacggta tctaacaaca attccttcgt
     2401 tgcaatattt attttgtttt cgctgtagtg aataaacgcc gatgtatacg cttgatgttt
     2461 cttcaaaaaa tacaaaagga caagaatact tgctagcata ccgataaatg ccgcgaaagt
     2521 tgattgcgtg accgccgtca catagtcacc atccatgact ttcatgatga tgaatgtagc
     2581 caatagcata taaaaaatac gtgccacctg ttctacaatc tgcgacagcg cataaggcat
     2641 catttcttga ttgccttgaa aataccctcg tatcacactc atacaaggaa aaaccaatac
     2701 agcgatactc aaagaacgca tcgttggaat cagttcttct ccgccaccag aagcatgagc
     2761 tagccaagga gaggcgatgt acatgaacaa agcaaacaac aggcctagcc cgcccatcat
     2821 ctgtaatgca cggataaata atcgtcgtga agtgccatat tcattcaatg aattatagcg
     2881 tgcagtttgt tttgcaatcg cagcaggtat tcctgctgta gagatcagta gaaacaatgc
     2941 atatatattg tatcccatat tgaataatcc attggcagcc ttagcatttt cgcccatcca
     3001 agcataccag ggaatgatat agacagcgcc tagcaatctt gaaatgatat tactggcggt
     3061 catccaagca gagcctcgtg ccattttttc ttgcggtgtt aattgatcta atgctggttt
     3121 tctttgatta tccatcggtc agattccaac tttctacaaa acattactat cattttaatt
     3181 ggtaaaacag aaactggcaa tcgattttat ataactttta taagaacaat tgtttttttg
     3241 aattcggttt cgtttttttg gtaaagtaaa gaaatataca gccttttatg gctaacattt
     3301 ataggagata tcaatgacac aagaaataaa aaaactaccc gttatatttt taggtctttt
     3361 ctttatcaca gttggattga attggttctt gctccctcac gatatagctt ccgccggagt
     3421 gggcgccatc ggacatttag ttgaaacggc cttttctatc caacgctcag ttactgtttg
     3481 gagcatcaat cttatgatgc tgcttctagc cgctgtccta ttaggaaaat cagtattttt
     3541 taagactgta gcgggaagtc tgctttttcc tgttgtttta gagattgttc caaaagtgga
     3601 aatgatctcc tcacatttct tttcactgat ttcaggaagt cttttgttca gtcttggggt
     3661 ttacacattg tacacagtcg gggcctcaaa tggcggtata acgataccgc cgattatttt
     3721 ccacaaattt tttcggcttc caatgccgca aggactttta cttaccaata gtcttatcgt
     3781 ctttttgaat tattatgttt ttggattgtt ggagacacta ttcgttctcc tatctattac
     3841 agtcagttca ttgtttatga aaatactcat ccgcttgtcc cctgtgtcaa gaaaatttga
     3901 ataaagctgg attttgtcat agcggaaaca aacgatatat taaaaagagt gtgctataat
     3961 aaaccaagaa attgaataaa gcgagggatc aatcaatgag cttctcttta gaagacatcc
     4021 gtcatttact attaaaagaa aatctattaa aagaatttgt ttcaacaaga ggctggcatc
     4081 ttgatgtacc agaaaaagct gaatttcttc agttaagtta tgattcaaga aaagctgatg
     4141 cttctacttt gtttttctgt aaaggaatga atttcaaaga agaatatttg gattcagcaa
     4201 tcgaacaggg tattcaatat tatgtatcag aacaaccata cgaaaattca gcagtaggaa
     4261 tcatcgtaac ggatatccgt aaagcaatgg cgcttcttgc tatggctttt tacgactatc
     4321 cgcaaaataa attgaaagtg atcggattca ctggaacaaa aggtaaaaca actgctgcgt
     4381 actttacaaa agcaatcttg gatcacacaa caaataaaaa aacagcattg ctttctacta
     4441 tgaatacaac gttagatggc aaaacatatt tcaaatccca tttgacaacg cctgagtcat
     4501 tagatttata tcgtatgatg tcagaagcag ttgaaaatgg tatgactcat ctagtgatgg
     4561 aagtttcttc acaagcttac aaaacacaac gcgtatacgg cttgacttta gatgtcggaa
     4621 tcttcttgaa tatctcgcct gaccatatca gtccgatcga acatccgaca tttgatgact
     4681 atttctattg caagcgacaa ttgatcttga attcaaaaac agttgtttta aatcatgaaa
     4741 gtgactattt tgatttactt agggaaacag ccgatctatt ccagattcca acgatcactt
     4801 atggtcgttc ggaagaaagc aattatcaag tgatccgttc agataaaggt actcacggtt
     4861 ttactttatc ttctcatgaa gatcgcttag ctatctgcga tactgcttat gatattttgc
     4921 tggctggagg attcaaccaa gaaaatgcag ccagtgcgat cattgccgca gcactaacag
     4981 gtgcctccag tcaagatgca caagacggat taaaagaagc tcgtgtacca ggccgaatgg
     5041 atcaattgat ccaatccaat ggagcgcatg tctatgtgga ctatgcacat aattatctaa
     5101 gcttgaaaac attgctggaa tttgccaaaa atgaacatcc agatggccgt gtaattgttg
     5161 tattaggcag cccaggaaac aaagcaatct cccgtcgaca tgattttgga aaagtgctat
     5221 cagaaacagc tgacatcgcc tttttgacgg cagatgaccc agcatttgaa gatccgaaaa
     5281 aaatcgcgga agaaatcaat gaagcaatta ctaatccaga tttgatcgtt cattatgaaa
     5341 tggatcgtcc tgaagcgatt cgacgcgctt tagcagaaag cgctccgcaa gattcagtcg
     5401 ttattgcggg aaaaggcgtg gatccctatc aaaaaatcaa tggtgtagat gaaccttatg
     5461 aaggggatta tgcaatcgtg aagcggttga ttgaagaata atggtattta tatactagtg
     5521 gtttcccatt tctaagaaaa aaagggactg tgattttttg tcacagttcc ttttttctta
     5581 gaaatcattt taacttttca ctagctaatt ctgctgcctt ttctctttct tcatttaata
     5641 atttattatc atagaatttg ataaatggga accagataat gaatgcgatg attgcacaaa
     5701 tgactgctaa cacagcagct ttccaatcac caccactacc aacaaatgct ccgatcccga
     5761 ctggtgaagg ccacggcatc tgtgcaagca tcggtttgac gatttccagc cgaatggcaa
     5821 aataagcgag tgtagcagaa gccatcggag ctaagaaaaa cggaaccgct aaatagggat
     5881 tgtagacgat tggcatcccg aaaatgattg gttcattgat attgaataat cctggtacaa
     5941 gtgaggcttt tcctaacact ttcagctgat cagatttcgc gaggaatgca ataaagatga
     6001 tcaatcctaa agtcgcacct gatccaccaa cggttacata agaattgttg aattctcctg
     6061 ccaacggata atttgcgcct tgtgcgttag ctgccatatt agctaatacg atcggggtca
     6121 agaaagaagt gatgatattc gctccatgga tccctacgat ccacaatgca tggatcaaga
     6181 aataaatcac catgacacca agccatgtat tcgtcaactg cgtaacaaac ccaaatggga
     6241 tagcaatcat tttgaagata tccgttccta gtgcaactaa taatccattg atgatcatca
     6301 ccacaaacgc aatgacaaat gttgggatca gagcagtgaa agaacgagaa actcctggcg
     6361 gaacaacatc tggcattttt atgacccaat ttctcttgac acacatacaa tacaattcga
     6421 ccgcaataat cgacataata atagctgtaa agattcctga agttcctaaa cgttcgacaa
     6481 aagagcccat cctaatacca tttactacag tttctgtctc attgatagaa gcaatcaagc
     6541 tgattttacc atcagtcatg atcaattcag ggatacacat aaagaatgca aaaacagata
     6601 gtaatgcccc cgtgagcgga ttaacattta atttttgttc atctcgttca attgacgtta
     6661 attcataagc aaacacaatg gcaaagtaaa tagataaaat ccccatcgtc attgtattga
     6721 cgatcatgta caagtcacta actttgaaaa aagttgcatt aaaaaatcct tctaaaaatg
     6781 ggaacggtaa tggcaaaata tttaaaacca aaaacatcga gcctacaatc gtgaaaggaa
     6841 tagaagccat ccccgctgcc atgacagccc gaacgatttt aagttgcgca attttcccca
     6901 taggtcccat caggtatttc tgcaaaaaat caaacattag ttattctcct ttcctaaata
     6961 taacgctttt tcatattgtt taatacggat atactgttgg tcttggttga gtgaaatttt
     7021 ctttaaacga tactgtataa atgcactggc aaaaataccg cttacaacaa gaatgaaaac
     7081 aaataactgt cctccattgt tatttgccat aaaaggatac aagaatgtaa atcctgaggt
     7141 aaacaacact ggaatcacta ataaattaaa gacactctgg aacaaaaaat accactttgt
     7201 ccatggaacg ttcgtttggt gttttcgata aagctttact tgttctccta ctgcaggcaa
     7261 aatagctaaa atcagaatca tcggtaaact acttcctaaa cttttagctg atagaagcag
     7321 aatcatccat tgcatgttca tgaaaaagaa aaaggcagtc aggtaacgta cgattagata
     7381 acgattgaaa tacatgtttt tcaaactaag ttgctttttt tctaattcga ttttcctttt
     7441 tactgtatcc tgcacaatga tccttccttt ctatttattt ttttgataca aataattcat
     7501 ttcctgtgca acttccaata atgtcatcgt cgtcatcaga tgatcttgtg catggaccat
     7561 gataatttcc atttctattt ttgtcccgct ggcatattct ttcagaagtt gagtttgtga
     7621 ctgatgcgca tgaagaatct cttcatttgc ttgtcgcaac ttctcttcta cttcttcaaa
     7681 catctcttta cgcatcgagc gaaatgcttc atgtatcagt gtccgtgcat ttccgctatg
     7741 gaaaatgata tcaaaagaag tcacttgtag tgtttcactg tttatttttt cagccatctg
     7801 ttttcctcct tattcgatca gtttgataaa tttactgctg aaattctcaa aaataggttc
     7861 agctaagatt tcctgctgta cttttagctg gtcaactaac ctaacgattg ctttagtcac
     7921 gaccgtaatt ccttcatttt cgacataaga aggcgaaact aaaaaaacaa aatgaatttc
     7981 ttgatattga tcccactgca tcccagaagg aatcaatgca acggctacct ttgtttcgat
     8041 tccaactgga acagcgggat gtgggactgc cactgtatca ctgaatatga tttgtcccat
     8101 attttcacgt tgctggatct gctttcgcat ttctcttata tatccttctt cttcatttat
     8161 gctcagcttt tgcagcaaat catgaagaat ttcttccttt ttcggttcgt acgtataagt
     8221 cttgaaaaat ttttcagaca actgttcggt gtaaatctgc tttttttgct gtagagaaaa
     8281 tgctggtatc ggtctatctg aggaatgata gggtatagat tctccaacta cctttcgtat
     8341 tttacgaaca tcatcttcat tcaaaaaaat actgacatgt aaaactggaa ttttgaaaaa
     8401 cattgtcgaa agatcaatag aagaaataat caggtcgatt ccttttaaag ttgtttcatt
     8461 gatctcataa tagcctttta cacttaccac tgtaatattt ttaccgaatt cactgactac
     8521 gcgattcttt aatagttggg cactgccgta acccgttgca cagataatca atgcatgtag
     8581 tttatgcgca gctctttctt tttccaatgc cgccatgaga tgcaaagcca aataggccca
     8641 ttcatcttca ttgatggtat agtttttcaa agccggcatg ttgctgaaat gcctttttac
     8701 tgcgtcaaag actttgggat tttctttctt tatctcctca gtcaatgggt tctcaatgcg
     8761 aatacctcgg ctcaatcgaa tcatcatcgg tgacaaatga tcgagtagtc cattgaaaag
     8821 ctggagatcc tctactaatg aaaaatgaaa ttcctgagac aatcttttca gaactttcgc
     8881 taattccttt gatagttcct gatcattttg attttctttg tgattcgact tagccatgag
     8941 atgtaatgtc aaataagcaa tttcttcttc gggaaacgat acacctgcga ttacctcaat
     9001 acgttgaacg atccgctgcg ccacttgata ctctactcgc ttagaaactt cttgttcgat
     9061 gcctaattcc ttgatttcaa acccttctcg gagccgtttg atccccaaag ccaagtgtaa
     9121 agtcagattc tgtatgataa aatccgaaac ttttaactta gcttctcttg tttcatcgag
     9181 aatgataatg actagctctt caaaactgat gtctgggaaa aaacggcttt gccctaaata
     9241 ttctttcaat gaatttccat aatggtttcc aaaaaaagta tccatgataa aatgccgtcg
     9301 atttctttct tccccttcga tccatgtccc ttgaccatgc ttagaaacga tttttagtga
     9361 atatggaatc aatcgctcct tgatttcttg catgtccttt gtcaaactgg aacgactgat
     9421 ataaagctct tctgccaaat catccataaa aagaatggca tcttccaaaa gcaattggtt
     9481 caatatatac ttttgtcgat cttcggactc ataaaatcct tgcggttctc tagcatatcc
     9541 aggtactatt tcttgccgac tcaaaaacaa gtcaaatgtc agtctttgta aaatatgcag
     9601 ttggtagcca taacctggtt tagcaatgat cttacctccg tttttttcta ctaactcttt
     9661 gatccgctgc aagtaattac ggattgtccg atcagataat aacaattcag aagctaaacg
     9721 ttgactggtg acatactcct tcttgtgatc aatcaaatat ctcaacagca atttttcctt
     9781 ctgtttcatt atcatccact cctattccat catatgttct cttcgatcac ctctgctaac
     9841 ttctggatgc ctgtagggat tgggacatac gcttggaaag ggatactaac aacaggcttt
     9901 ccaacagatt ctcctaattt tttgaattta tccaaaaaca tcgtcgtttg cgggctgatc
     9961 agaaataaat caaaatcact attcttaatc atattatcgc cttctgtagc tgaaactgca
    10021 tctacgagta cctcttctct tttttgctta aagaaatccg ttgtctttga ggccatcatc
    10081 gaggatgaca ttcctgccgc acaaataatc aatgcttttt tcataaatca atcactcctt
    10141 ttatttttat aaaccaagca tatcttgtaa gcgttttcta ttcaaaatat atatttccgt
    10201 agcattgcgg aaaaaatgtg tatcatatct tgaataataa tttctggcac cgtgatatac
    10261 tagtgataga attttttcag gaggggattt agtgagtatc acagtgaata ttttgtatac
    10321 aagtacaaat ggacaagcca gaaaatttgc agaagaaatg gtcgaaagaa acattgtcaa
    10381 agcgattcgt gcagaagaag gaaacgagaa atatgactat tactttccac tagaagatcc
    10441 agaatctctt ttactgatcg accgttggaa agatgaagaa gcaatcgaaa agcatcacaa
    10501 atccgaaatg atggctcaaa taaacgaact ccgtaaaaag tataaattaa aaatgaaagt
    10561 cgaacgtttc actgatttac cgtaaaaaga tacacatgga atgatagcaa tttatcatcc
    10621 atgtgtatct tcactttttc cttatttttt attcgatctc atctaatgat tcaaaccgtg
    10681 tttttctctt atttttcgga taagtatttc agtcacccca atatacatga ttcctaaaca
    10741 taccccaagg atattctcaa aaatgcggat cgttactgct cctggaaccc cgaataaact
    10801 ggcagtgatt gccaaagcac caaaagaatt aaaaactgtt ttatatctat aggtagaaca
    10861 aacacctaaa gctagaccac ctaatatacc gacccagtta aaaggaataa attggctgat
    10921 accaataaat aaaacacatc caatcaatga gccaatgatt cggtctactg ctcgttcttt
    10981 gaaaccaatc gacatcaatc catagctaga tacaatagaa gaaaatgcaa accctgccca
    11041 catcaatcgc tgaaaaggga catactctcc tgcaacgatc aaaagactaa tgccaaatgc
    11101 atagatgagc atccaaattt tttcctgcga atacatgcct ttcaaaaaaa tctctttaaa
    11161 caagcttttg tctttgttct tttctcgatg ttttctgtaa agaagaactg aaaaccaaca
    11221 aaaaaacaaa aataataagc tacttgtttg tgtaaagaaa ttcttgttca acgaatcttt
    11281 aggtaacgaa taaacaataa agagatatga aaagccgtac aaagaagcat tccccatttt
    11341 gggatcacta gcggttgcca ttaatatgct gcttagtaaa ataaaatgaa tcaacatgcc
    11401 cataactgac catttgatca gctggacata cggtgctact aatagtgaga acatgacgat
    11461 tgctaaacct atgatcgaat gggaaatctt ataaccaaag tcaacaaaac gaatactcat
    11521 caacatacag aataaaacca cagcgtatgg tttatgcttc gattcaaata atgttgtaat
    11581 tataatgata aaacaaatgg caaacaatac gatgaataat gatcgtaaaa acaatgaaca
    11641 aatatacatg cacttttctt ttttagaagc tgcttctcgt attttttgtt ttaaaataaa
    11701 cggatctagt tgtagagact gataaaaatc cacctgttgt cgtcctttct ttttactttt
    11761 attttttaca gtcatctatg acaggttgta aatactatgg aacatcatat ggcgttgtca
    11821 ttttcttgtc aactattttt cctattcatg tttttataaa aaaacatatc gttttcttgt
    11881 atgattaaat caccacaaaa taaacaactg agagaacgat atgctttatg aatggttcta
    11941 tcaaaaaaat tctgagatca atagacaaaa atttgatgat tacagaggtc tcttacgaaa
    12001 ccttacagaa gaaaaaaaca ttgatcgttg ctgcagttct cttccctgct ccatgtgtct
    12061 gtagaaattg tggttctgct gtggtagatg gaaataggaa attaatggtg gtgaaaaaca
    12121 agaagaagga aaccattgtc cactttgaat aatataatca tatgccaatg gttatacgcc
    12181 taaaaaagca gcgctatacc tgtaaaaact gtcgaagcca ttggaacgct caaagttatt
    12241 ttatccgtcc aaggcactcg atttcaaatc atgtcagaca taaaatcact tctttactga
    12301 ctgaaaaagt gtcgttattt tttatttcga aaagttgtta ggtatctttg acaaccgtta
    12361 ttcgcacatt gaaagagttg agaagctatt taccaaagtc ttccaaaaga attctgtctt
    12421 gagtgttgag ggttgtgaat ttcgttctca cgtttccgtt gaggataaac tgagttttat
    12481 ttccgcggat ggcgagacag aaaaattaat cgagaaaatt tacctacaca aaaaattcct
    12541 cgattaacaa actatttcct tagctgcact aatcctaaag aaatagaatt cttagttaca
    12601 gacatgaacg ccacctactt tcagctcacc aaacatgttc taccaaatgc gaaattggtg
    12661 attgatcgat tacttggttt ttccacgcct ttcaaggagg cgtaaccttt attcacgagt
    12721 tggttgaagc tttccgagac aaaagacccg acttgttttt ctccttattg acaaagagcc
    12781 attaaaacta gagaaaaaat tacaactgat cgctgattat ttgtagaaag aaaatcaaag
    12841 tattacccaa taaacttgag tcattaggca aaaaacactg cgaaactcaa gaatgatttt
    12901 cgcagtgttt ttgtatattt tttattaact agtattacta caaaggttta tttttcactt
    12961 ctcaacgcag cacaaaattg tctttactta tttactggtt gggttcaacc ttctctactc
    13021 cgatactaat atttgtttgt ataccccttc taaaatcggt agtttccatt caattgatgg
    13081 gtactagttg attttaaaaa ctatgcttat tcaatttttt attttagctc atatatttcg
    13141 tttctttcta aattttatac tgatgaaaac aagtagcaca cccaatattg acaatataaa
    13201 ctggctaatg atggtttctc cagtttttgg aagatgctta tttccactgc ttgttggatt
    13261 gtttcctctt atatctgagc tatcagcaac ttttctattc tcttttgtta aaaagatcgc
    13321 cacttcttgt tctttttcga tcttgaaagt tactggtgtc gcatccaatt cataccccgt
    13381 tggcgcttga gtttctacca gctgatatgc tcctggtgct aagccatc
//
LOCUS       DAJSKS010000071        13212 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJSKS010000071 DAJSKS010000000
VERSION     DAJSKS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.073"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            147..1016
                     /locus_tag="ON396_001732"
     CDS             147..1016
                     /locus_tag="ON396_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HCR3113817.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ON396_001733"
     CDS             1225..2250
                     /locus_tag="ON396_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCR3113818.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ON396_001734"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ON396_001734"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCR3113819.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="ON396_001735"
     CDS             3333..5036
                     /locus_tag="ON396_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3113820.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ON396_001736"
     CDS             5038..7071
                     /locus_tag="ON396_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR3113821.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ON396_001737"
     CDS             7082..7522
                     /locus_tag="ON396_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3113822.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ON396_001738"
     CDS             complement(7722..8849)
                     /locus_tag="ON396_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3113823.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ON396_001739"
     CDS             complement(8852..9529)
                     /locus_tag="ON396_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3113824.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="ON396_001740"
     CDS             9793..11559
                     /locus_tag="ON396_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR3113825.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="ON396_001741"
     CDS             11574..12011
                     /locus_tag="ON396_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3113826.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="ON396_001742"
     CDS             12024..13175
                     /locus_tag="ON396_001742"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR3113827.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4488 a         2293 c         2731 g         3700 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DAJSKS010000072        13107 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJSKS010000072 DAJSKS010000000
VERSION     DAJSKS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.074"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            74..1813
                     /gene="yfcC"
                     /locus_tag="ON396_001743"
     CDS             74..1813
                     /gene="yfcC"
                     /locus_tag="ON396_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR3113828.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1970..3154
                     /gene="iadA"
                     /locus_tag="ON396_001744"
     CDS             1970..3154
                     /gene="iadA"
                     /locus_tag="ON396_001744"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR3113829.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3265..3468
                     /locus_tag="ON396_001745"
     CDS             3265..3468
                     /locus_tag="ON396_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR3113830.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3569..4231)
                     /locus_tag="ON396_001746"
     CDS             complement(3569..4231)
                     /locus_tag="ON396_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3113831.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4244..5746)
                     /locus_tag="ON396_001747"
     CDS             complement(4244..5746)
                     /locus_tag="ON396_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3113832.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6294..7973
                     /locus_tag="ON396_001748"
     CDS             6294..7973
                     /locus_tag="ON396_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR3113833.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8100..8579
                     /locus_tag="ON396_001749"
     CDS             8100..8579
                     /locus_tag="ON396_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HCR3113834.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8798..9478
                     /locus_tag="ON396_001750"
     CDS             8798..9478
                     /locus_tag="ON396_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR3113835.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9486..10817
                     /locus_tag="ON396_001751"
     CDS             9486..10817
                     /locus_tag="ON396_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR3113836.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10832..11794
                     /locus_tag="ON396_001752"
     CDS             10832..11794
                     /locus_tag="ON396_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HCR3113837.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11905..12243
                     /locus_tag="ON396_001753"
     CDS             11905..12243
                     /locus_tag="ON396_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HCR3113838.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12322..13029
                     /locus_tag="ON396_001754"
     CDS             12322..13029
                     /locus_tag="ON396_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCR3113839.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4162 a         2317 c         2878 g         3750 t
ORIGIN      
        1 ttactgtata attcatttga ttatccattt tttattcatc aaaattactg aaaaaagaaa
       61 ggaagaaata aatttgaaga aaacaaattc ttcaggaaaa ccgaaacgcg acttcactaa
      121 attatcgaca ccccatacgt atgtcattat ttttggtgta gtcatctttg catggatact
      181 gacttttgtc gttccagcag gaaagttcag cacgcaagat attgaataca aagatgcaaa
      241 tggcgaaaca agtactcgta ctgttttacg tcaagattcc ttccgttatg cttacgagct
      301 ggataagtcg tatgtgtttg atcaattaga agaactgcaa gatcatccag cagaacgtga
      361 aaaactggat gttccggaaa aaggattgga aaaagtcatt gctgacggag aaaaaaatct
      421 tacgcaggaa aaactagatg aaatctcctt gacagatgat gtgctttacg atcagtacgg
      481 cgaaaatatc tatgatactt ctaaaaaatt gcataaaaca gccaaaatat ggggaacaga
      541 tgattttggc ggctttggtt tcttaaattt tgtttttgaa ggactagtat ctggagataa
      601 gtatggatct gctgtaggga tcgctgcatt gatccttgtt gttggaggtg cttttggtat
      661 tattatgcgt accggagcga tcgacgcagg tatctatgcg tttatcagta aaactaaggg
      721 actggagcgt ttagctttac cgctattatt ctttgctttt tcattcggtg gcgctacatt
      781 cgggatggca gaagaggtaa ttccattctc aatggtgatg gttccatttg tcattgcgtt
      841 aggatatgac tcgatcgtag cagtaaccgt gacttatgtc gcatctcaag tcggaaatgc
      901 gacctcttgg atgagcccgt ttagcgtagc ggtggctcaa gggattgctg gaatccccgt
      961 gttgtctggt gcgacattcc gattgattat gtgggtagtt gttactgcat tagccgctgg
     1021 ttatatgatg atatatgcag aaaaaatccg taaaaaacca gaaagttcgt tgacttacaa
     1081 atcagatgat tatttccgtt ctcatatcaa aaagacatct gatgaaaata aaaaattcat
     1141 gcttgggcat aaattgatct tattagagat gttagttgtc cttgtctgga tcgtttgggg
     1201 cgtgactcaa aaaggctatt acattccaga aatcgcttct caattctttg tgatgggatt
     1261 agttgctgga atcattgctg ttcttttcaa attagatgga atgaaagtca atgacatcgc
     1321 ttcttctttc caatctggtg cagctgattt agctggtaca gcaatcgtag taggtatggc
     1381 caaagggatt cttcttgtct tgggaggatc tgatgcatct gttccttcag ctttgaatac
     1441 gattttgcat gggatcggga cggcgttgac cggtgttcca gctgtcattg gtgcttgggc
     1501 gatgtacatc ttccaaagtt tgttcaactt ggttgttaca tcaaattcag gacaagcagc
     1561 tttgactatg ccaatcatgg ctccactggc agatttagta ggtgtatcca gacaagtagc
     1621 agttcttgct tatcagcttg gttcaggttt tatggacgct tttacacccg tatcagccag
     1681 cttgattggt gtgctaggtg ttgcacggat cgaatgggca aaatgggcaa gattccaaat
     1741 caagatgcaa ggattcctgt ttgttttagg aactattttc atcatgattg ccattgcaat
     1801 aggtctgcag tagtactgga gacctgtaaa taaaaaagta atccaaacag tagataagga
     1861 ctccaagaac cgtcatttta cggtaaaatc aaaggagtaa agatcctatc gtttggatta
     1921 ttttttaaga tcctatcatc agttatactt aaagaaagag gaaaaatgta tgaaaatcat
     1981 tcgacaaatt gaagtgtttg caccagatta cttaggaaaa atggatgtac tgattgccgg
     2041 tgggaagatt ttggctgtag aagaacagct agaaggcggc tacgaaggag tggaagtaga
     2101 agaactttca ggtgagggaa aagtactaac cccgggtttc attgactgtc atttccatat
     2161 tttaggtgga ggaggagaag gcggttatca aaatcgaacg ccagaagtca cattgagcca
     2221 attgactaca gctggtgtaa cgacagttgt cggttgtttg ggaacagacg gagaaggtag
     2281 agatatgact gcgttgatca gcaaagcgaa aggtctggaa gcagaaggaa tctccactta
     2341 tgtatacgaa ggttcgtacc gattgccggt aaaaacagta acagattcta tcatcaaaga
     2401 ctttttgaca attgataaaa ttatcggaat cggagaaatc gcagtatccg atcaccgttc
     2461 ttctcagcca agttttgaag agttcgctcg tgcagtagca gatgctcgtg taggaggcat
     2521 gttatccgga aaagctggga tcatcaatgt ccacttaggc ggaggaaaaa gaaagctaga
     2581 gctattgaca cgaatcgtag aagaaacaga aattccgatc acacaatttt tgcctactca
     2641 tgcaaatcgc acaccagaat tatttgaagc ttgcgtggca tttgccaaac gtggtggcac
     2701 gattgacttc actgctagcg aagatcctga tttttgggag aagacagatg gtgaagttcg
     2761 cttcagcaaa gcactgaaac gattgatcga ggaagatgtt tctttagata atttcacgat
     2821 gacctcagat ggacaaggaa gcttgcctta ttttgatgaa aacaatcatt tcctagggtt
     2881 aggagtagga agtgccaagg cattgctagt agggatcaaa gaagctgtgc aaaaagaaag
     2941 tatccctttg gaaatcgcct tacgagctat aaccagtaat ccagcacgta tattgaaatt
     3001 ggataaaaaa ggaaaaatcg aaattggtgc tgatgcagat ctgtgtatct tggataaaga
     3061 aacgcttgat attgatacag ttatcgctaa aggagaaata atggtccaag aaaaagaagt
     3121 caaagtatgg gggactttcg aaaaaagttt ttaatcaaca gctctaacta tcttttttag
     3181 aaaacgagcg ttctgtttgt ctgtatctgt tgttgatgtt accctgaagg tagatcaata
     3241 aacaaactaa gaggaggaag cacaatggca gatttaaaag gacgttctaa agatatgaaa
     3301 gataaagtag tcggtgaagg aaaagaagca tacggcaaag caacagacga caagagcaaa
     3361 gaaacagaag ggaaagcaca atcactaggt gctgacatca aagaaaaagc tcgaaattta
     3421 ggcgacgaag tcaaagacaa gttttctcga aaagacgaaa aaaaataaaa accacttatt
     3481 actaattgaa tcaagcaaat aaaaaatccg aaccatatct gcgagacaga tatggttcgg
     3541 attttttatt tgatcattat cctgtttttt aaacttcgat tttttgaaat tttttatttt
     3601 ttagttcaaa gacttcatca cagctttttg ctacttcaat ctcatgagtc acgatgataa
     3661 tacagcgatt ttgttcatgg gcgatttctt ggaaaagttt gacgatatct tgtgaagttg
     3721 tttcatctag atttccagta ggctcatccg ctacgattag ttgatgttca cagcagatag
     3781 cacgaacgat agcgactcgc tgctgctgac ctcccgaaag tttggtcacc tgtttttttg
     3841 ctaatatttc atcaattcct acacgagcca gtcgatctaa agcaacttgt cgataattct
     3901 cttgcttggt cttagtgatt tccattgctg ctatgacgtt gtctagagcc gataaataag
     3961 gaagcaagtt gtaagcttgg aaaataatcg agacatcatt tttacgataa cttcttaacc
     4021 cgattttgtt cagtgaacga tcttgatagt atacctttcc ttcttttggt gcatctagtc
     4081 cagaaataag cgaaagaaaa gtcgtttttc cagaaccact tgctcctaaa atagcgtaca
     4141 tttttccagc ttcgaaattc agttctacat ctttataaag cgcatcttgc ggatcttgat
     4201 accaaaatcc caattgttct gtccgtaaca tcaaattcct cctttagttt aataggattt
     4261 ttcttgggtt taatcttaat atccctatag aagctaaaaa gacagaaaac agactgattc
     4321 caaatgctac tccggctaat agtcccaatt gtgcaggttt gacattgatt tccaattcgc
     4381 tgacttgatc agaaacagca aatggattat tcgtggttcc ttgttgacca tcgcgttgac
     4441 cgccttgttc ttgtctgtta cctggcattt gtccaccgcc aggagcttgc tggttttgtg
     4501 tcgtggtttc tgtctgttgg tcaagtagct gctgtcccac ggcattcgcg acgacgtttc
     4561 cgctaagcga agcaactcct aatgcaagaa tcatacaaat agccacttcg gtaaacaatt
     4621 ggagaatgat tttacttctg gattctccta aagatagcaa gacgcctaac tcatgccgac
     4681 gctcccgaat ggtaatcata atgatcagag taagtatgat aataccggct acagtcacta
     4741 gtaaaacaac atttttagaa aaacttgcca cgttgtttaa aggttccaac attgactgat
     4801 acatggaatc atttgattgt aagctaaatg tatctgtgtc gatcagcttc tcagcttctt
     4861 tgagaaactc atccatttta tctggatcag atagagtgta aatagcagaa tctatcgtgt
     4921 tatcgtcaga tgtcccattt agttcatttg caaaagtgta agaagtgtaa agtgtattcg
     4981 acggattcat gaagttgaat ttcataccca tactgccgct tgtttcgctg gattcataaa
     5041 ttcctttgat cgtcatttca taagtggtat cttcatcttc agtactcgtg attgtgaaag
     5101 tatctccgac tgccaggtca tttgtttctg ctagagtaga atcaatgact acattgtttg
     5161 tgcctttgtc gctgtcatct attccttcac catccgtgat ttttgctgtt ccatcagaaa
     5221 aattactatt ttgtgcagtt gctgacacac cactgatttg aaagtctgct tgagtcattt
     5281 gcggattccc gcccattcct ccagcaggtt caccgcctgc atcagtagtt tctgaactgt
     5341 cttcacttga atcagaacta gtgatggcag ttattccatc ttttgccata gctgaggcgg
     5401 aactttcaaa ggaataactc tttacattat ctaattcagc tattttttcc gcgtccgtta
     5461 aggaaactgg tgtaagagaa aaactgcctg gatctggacg ttcttcagtg gtagaaccgc
     5521 ttgaagaagt ttgttcctgt tttttaaatg aagcttctcg attggcactt aaagtcactg
     5581 ttgctccaac ttctttttgt gcttgattcg ctgcttcttc tgcagcgctt cgaatcgtga
     5641 ggccagctag cacaaaaata agaatggctg tgaacacggc aaacaataac aatgaacgtc
     5701 cccattttac ttttgtactt ttccatgcac gtttcataaa attcatattt tgcatccctc
     5761 ctagtagatg catttgataa tagacaacct atcttaaagc tagcttaaat cttatgctgt
     5821 ttgcacacca tcaacatgag gttctgtata tataaaaagt cactatagtt attgcaaaaa
     5881 atgtggtaaa atgaaaaaag tatgtttcaa accgacgaag aagaaatgaa agaggaatag
     5941 caaaagaaat cctagatgct acagagggat ttttacgtgc atagttgttc taacaattgc
     6001 tgatactagc gatttagttt atttgttgta taataatggg gaacagctga atacttatat
     6061 aagtattctg gctatagcgt ttcgcaaaag atccattttg ttaatgaagt atatccaaaa
     6121 taaaatgccc atccaactgt cagaacgcgg atgtgctggt aattttcctg ctgattctcc
     6181 ataagttgag aaatagtgat tttgcatatg cttcattgga atggctcata tatttagata
     6241 aagaagaaaa tcgacatctt taaacttgga tgtcataaga agaggtgaag tttgtgagta
     6301 ccattaaaat tatcccatta ggcggcgttc gtgaaaatgg aaaaaacatg tatgtcgcag
     6361 aagtgggcga ggatattttt attttagatt gtgggctaaa atatcctgaa aatgagttgt
     6421 tggggatcga cgtcgtaatc cctgatttta cgtatttaga agaaaacatt gatcgtgtag
     6481 ctggtgtctt tttaacacat ggacatgcag atgcgatcgg ggcacttcct tacttgttgt
     6541 caaaaatttc tgtaccagta ttcgggacag agttgacgat cgagttagca aaattgaatg
     6601 tcagtcgtaa tgaagcagcc aaaggattta aggatttcca tgtggtagat gagcatacag
     6661 aaatagactt tggtgaaaca gtagtgagct ttttccgtac cactcatacg ataccagatt
     6721 cagtggggat caatctgaaa acaaaagaag gtagtatcgt ttatacggga gattttaaat
     6781 ttgaccaaac ggctatccca atgtatcaaa cagattatgc acgattggcg gaaatcggca
     6841 aagaaggagt cttagcctta ctaagtgaat cggcaaatgc agaaaatcct cagcctgtag
     6901 cttctgaagc ccaaatagca gatgaagtat ttgatacgat caaatattgg gaagggcgta
     6961 tcatcgtcgc ttgtgtagca agtaatttgc aacgagtgca acaagtattg gatgcagcag
     7021 aaaaagcaca acgaaaagtt gtcttgactg gtcaagattt cgaacgaatt attcgcacag
     7081 cgatgcgttt agaaaaactt caattaccaa gtgaagatct gcttgttacg ttgaaagaca
     7141 tgaaaaacta tgatccagaa gaactgatca ttttagaaac tggccgaatg ggtgaaccca
     7201 tcaagtctct gcaaaaaatg gcaaacggta cacaccgtaa tgtcaagatt gaagaaggag
     7261 atttggttta tatcactact acgccaagca tcgcgatgga aaccatcgtg gcgaaaacgg
     7321 aagatatcat ttatcgagct ggtggaacag tgaagttgat ctctgagaat atgcgcgttt
     7381 ctggccatgc caatcctaat gacttgcaat taatgatcaa tttgatgaaa ccaacatatt
     7441 ttgtgccagt tcaaggagag tatcgtgaat tagctgctca tgcagatttg gctcatgcag
     7501 tagggatgcc atacaaaaat atctatatta ctggacgcgg agacgtgctg gaatataaga
     7561 acaagcgtat gagtgttgca ggaactacaa gtgcagaaaa tgtaatgatt gatggattag
     7621 gcataggtga tatcggaaac attgttttac gtgatcgaaa aatcttgtca gaagacggga
     7681 ttttcgtagc ggttgtcact atcagtcggc gagaaaagaa aatcatttca aaacctcaaa
     7741 ttacttcacg gggattcgtt tacgtaaaag ctagccgtga tctgatgaaa gaaagcagca
     7801 atatgattga aacaatcgtt gagaaacatt tagaaagcaa tgattttgag tggagcaaat
     7861 taaagcaaga catccgagat caattaggcc gttacttatt tgaacaaaca aaacgccgcc
     7921 cagtgatctt gccagtcatt atggaagcta ctcaacgtcg aaataaaaaa taaacaaaaa
     7981 gagtaagatg aacctctcta tagagtgctc gtcttgctct ttttttgcga aaaacaaata
     8041 atttttgaat acagcatccc tcgctttata ataaacgata gaataaaggg agtgaatcaa
     8101 tgatgagtca aacaaaagtc gaacgttatt tagaagaaca aaagatcagc tatcaaccac
     8161 ttgattttag tcatgtggta gaagaatctt tcacagaaga actacaaaaa agagggattg
     8221 atccagcttt gatatgtaaa actctcgtag tgaaaggaaa caaaactggg gtgatcatcg
     8281 cattgattcc tttgaacgat cacttagatt acaaaaaaac gagacaagtg acgaaagatc
     8341 gtaaagttgg ttttcctgga atggattttg tattaatgca tacaggatat ccccacggag
     8401 caaacacccc tattggaata aaactcgcac atcctgatta ttgtttttta gcagacagtt
     8461 ctttaaaaga aaaagaagaa gtaattgtgt caagcggtga aatcgggaga agccttcgga
     8521 taaaagtagc cgatttagaa aagatcgtcc atcctattta cgctgattta attagttaag
     8581 ggacgagaag agaagctaag cagagcagag acctcaaact tgagattata tctgcttggt
     8641 acttggtgca tcctgtatta tctgttacaa ttgctttgta catactgtac tatcaatcca
     8701 atgaaaatat atttagcagc tatatttttt cattgatgtg caagtgatga aatcgttttt
     8761 aataaaaaag atacagagaa aacgcgaggt gtaaaatatg aaaattacgt atcatggaca
     8821 ttcctgttta acaattgaaa tggaaaatgg ccaacgcgtg atgattgacc cgtttattac
     8881 aggaaatcca ttgacagact taactgcaga agaagtaaag acagattggt tgctgattac
     8941 tcatggtcat agtgaccaca taggagatat gattcctatt gcaaaagaaa atcaagccac
     9001 aattatcagt attgttgaga ttgctgatta tgcgaacagc agaggagtag attcattcgg
     9061 tatgaatatc ggtggaaaac atgcgtttcc attcggcgtg gtaaaattcg tacatgcaca
     9121 gcatagttca agctatgaag tagacggtat cgttcagtat atgggcgagc ctagcggaat
     9181 tattattcaa gcagaaggaa aaacgattta tcatgcaggg gatactgctt atttcagtga
     9241 tctaggattg cttgcagaag aattcgatat tgatgtcgct ttcttgccga ttggcgataa
     9301 ctatacgatg gggccagaag atgcagcaaa ggctgcgcaa acaattcgtg ccaagaaagt
     9361 agttccaatc cattacaata ctttccctgt cattgaacaa gatcccgaat cttttgtaga
     9421 gttactggaa gatagtgaag gaaaaataat gagcgtctcg gaaacgctgg aagtgtaggc
     9481 agaaaatggc gacaaaacat gatttgattt tagaatatat tgaaagttta cctgttggca
     9541 atcgaatttc tgtaagaagc atcgcaaaag aattgaatgt cagtgaagga acagcgtacc
     9601 gtgcaatcaa agatgcagaa aatgtcggac tagtttcaac aattcaacga gtagggacca
     9661 tccgtatcga acgcaaatta aaaaaacata ttgaacgttt gacatttggc gaggtcgtca
     9721 ggatcattga aggggacgtt ttaggcgggg cggcaggatt agacaaggtc ttgaataaat
     9781 tcgtcatcgg cgcgatgact aaacatgcca tggaacgcta tattacgcca ggatcactaa
     9841 tgatcgtcgg caaccgggcg gaagtccaaa aattagcatt ggaagatggc gccgcagtat
     9901 tgatcacagg aggattcaat acgactgctg agattgcaaa gctagctgat gatcttgaaa
     9961 tgccgatctt acgcacctct tatgatacat ttacagtcgc aaccatgatc aaccgagcgt
    10021 taagcgacca attgatcaaa aaagatatca tgcttgtcag tgatatctat atgccagctg
    10081 aaaagactca ttatttgcag cagacagata cgatttctga ttatcagcgt ttgtcagaag
    10141 aaacgcagca ttcgcgtttt ccagtcgtca accgccatca tcgtttgatg gggattgtta
    10201 cggcaaaaga tgttttaggg aaatcgccaa accagatcat cgatcgggtg atgacgaaag
    10261 aaccaatcag tgtaaagaaa acaatgagta ttgcctctgt tagccatcaa atgatctggg
    10321 atgggctaga agtcatgccg gtcgtttccg atgacttgac attggaaggc ttgattaccc
    10381 ggcaagatgt aatgaaagcg atgcagcttg tccagcgaca accgcagatt gcagatacga
    10441 tttcggatca gatatctgga aatatccata cgattgatac tgaccgtgaa ggaaatcgtt
    10501 tggaaaaccc taagttcaaa ttcgtagtag caccacaaat ggtcaatggc gttggaacca
    10561 tttcttttgg cgtacttagc gaaatcattt ctgatgtcgc acaaaaaaca atggtgatga
    10621 accaaaaacg aaatatcttg attgaacaag tgaacctaca ttatttgcgg ctgatccaat
    10681 tggaaagcga attagacatt cgtccacgtg tattggaaat cggcagacgt tccgctaaac
    10741 tggatatcga agtgtttatt gaaaacacca tcgtttccaa agcaatcgtt gtttgtcaag
    10801 taatggaacg ttcttagaaa gcaggagtga aatgacagta cagcaagaaa tagtagatac
    10861 aataaaaaaa tacgataaaa tcattatcca caggcatttg cgccctgatc ctgatgcatt
    10921 aggatcacaa gtaggattag ccgaactatt acgggaaagt ttcccagaaa aagaaatacg
    10981 tcaagtaggt gaaacgattg aagggcttcg ctttttagca gaaatgcaag aagttgaaga
    11041 tgtcttctac caaggagcgc tagtcatcgt gactgataca gctaattcac ccagaatcag
    11101 tgatcaaaga tatcagttgg cagaaaaact gatcaaaatc gaccatcatc ccaatgatga
    11161 cccttatgga gatcttgtgt gggtaaatac aaaagcaagt agctgtagtg agatgattgc
    11221 agaatttgct ttgatgtttc ctgaatcttt aaagatgaac gttaaggctg cacggttact
    11281 ttatgcagga atcgtcggag atacagggcg ctttttgtat ccctccacga cgtcccgtac
    11341 attagagatt gcttcgatat tgagaaatta tccatttgat gcaagtgctt tgaacagaga
    11401 gattgaacaa atgccgatga aagtcgctaa actttccggt tatctttatc aaaatatcca
    11461 ggtggatgaa aatggtgcag gaagaataat ccttccgcaa gaaattcttg ataaatatgg
    11521 aatcgatgat tcagagacag cttcgatcgt ttcacttcct ggagtgattg acgaagtgct
    11581 ggcttggggg atttttgttc agcagccttc agggtattac cgcgtacgtt tacgttctaa
    11641 ggggccgatc atcaatgaat tggctaaacg ccatcatgga ggaggccatc cattagcaag
    11701 tggtgcgaat gcaaaagatt tagcagaagt agaggaaatc tataaagaga tccaagatat
    11761 atgccaatcg tataagagca gaaaaacaga atgatacttc gttcgaaaac gtgccttttg
    11821 tagtaaaaat cacaaatgta tcttttcaag atcttctttt tccgttacaa tagtgctcac
    11881 taaagatttt aaaggagaaa gaagatgaca ttaccaaatt gcccagaatg tggctcagag
    11941 tacacatatg aagatcgagg cttgttgatt tgcccagaat gcgggaacga atggaacaaa
    12001 gaagaacaag aaactgtcat agaagacgga ttagtggtat tagatgcaaa tggaaaccgt
    12061 ctgttagagg gcgatagcgt gacagtgatt aaagacttga aagtaaaagg tgcgtcaggt
    12121 gccattaaac aaggaacaaa agtcaagaac atccgtttag tagaaggcga tcataatatc
    12181 gattgcaagg tagaaggatt tggaccgatg aaactgaaat cggaatttgt gaaaaagaat
    12241 taaagaatat tcaaggtctt ttcacattct tttcctacta ctaatgataa aataaagtaa
    12301 acgaaaagga gtgaaacgaa aatgtatggt tatggaatgt atggtttttt agatccgaca
    12361 tttcttttgg tcattcttgg attagttatt tccggaattg cgtcagcgta cgtcaatagt
    12421 actttccgaa aatacgatca agtgagaagc aaaaacaatg tgactggtac acaagcagca
    12481 caatatatat tacaaagtca acagataaat aatgtcggtg ttcaacaaat tgccggagat
    12541 ctcacagata actacaattc aggtaataag atgttgagtt tgtctcaagc aactgcacag
    12601 tctacttcag ttgctgcgat aggtgtagca gctcatgaat gcggacatgc ggtacaagat
    12661 gcttcgggtt atgtcccatt gaagattcgt gcagcacttg ttccagcagc taactttggt
    12721 tcgatgattt cgttcccctt gattatggta ggtgtattgc ttagctggaa ctctacgtta
    12781 atcaatatcg ggattttcgc tttttcttta gcattacttt tccaattggt gacattacca
    12841 gtcgaattta acgcgtctcg gcgagcaatc caaattttga gtgaaggcgg actactgacg
    12901 gaagaagaag taccgatggc aagacacgta ttatttgcag cagcactgac ttatgttgca
    12961 gcagcgcttt cgacattcct gcagctgttg cgcttgatct tattgttcgg cggcaaccga
    13021 agagattaaa ataaagaagt tgtgaaatca gcgctttgat ctgagctaat accgagagtt
    13081 agctgattga ataccattta tttggaa
//
LOCUS       DAJSKS010000073        13048 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJSKS010000073 DAJSKS010000000
VERSION     DAJSKS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.075"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(263..1711)
                     /gene="cls"
                     /locus_tag="ON396_001755"
     CDS             complement(263..1711)
                     /gene="cls"
                     /locus_tag="ON396_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR3113840.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1863..2756)
                     /locus_tag="ON396_001756"
     CDS             complement(1863..2756)
                     /locus_tag="ON396_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3113841.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2740..3426)
                     /gene="ftsE"
                     /locus_tag="ON396_001757"
     CDS             complement(2740..3426)
                     /gene="ftsE"
                     /locus_tag="ON396_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR3113842.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3531..4632)
                     /gene="prfB"
                     /locus_tag="ON396_001758"
     CDS             complement(join(3531..4559,4561..4632))
                     /gene="prfB"
                     /locus_tag="ON396_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR3113843.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4758..7292)
                     /gene="secA"
                     /locus_tag="ON396_001759"
     CDS             complement(4758..7292)
                     /gene="secA"
                     /locus_tag="ON396_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR3113844.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(7514..8065)
                     /gene="raiA"
                     /locus_tag="ON396_001760"
     CDS             complement(7514..8065)
                     /gene="raiA"
                     /locus_tag="ON396_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR3113845.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(8193..8912)
                     /locus_tag="ON396_001761"
     CDS             complement(8193..8912)
                     /locus_tag="ON396_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCR3113846.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(8909..10222)
                     /locus_tag="ON396_001762"
     CDS             complement(8909..10222)
                     /locus_tag="ON396_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR3113847.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(10303..10623)
                     /locus_tag="ON396_001763"
     CDS             complement(10303..10623)
                     /locus_tag="ON396_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3113848.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(10731..11018)
                     /locus_tag="ON396_001764"
     CDS             complement(10731..11018)
                     /locus_tag="ON396_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113849.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            11145..11789
                     /locus_tag="ON396_001765"
     CDS             11145..11789
                     /locus_tag="ON396_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCR3113850.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(11848..12555)
                     /locus_tag="ON396_001766"
     CDS             complement(11848..12555)
                     /locus_tag="ON396_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3113851.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(12775..12945)
                     /locus_tag="ON396_001767"
     CDS             complement(12775..12945)
                     /locus_tag="ON396_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113852.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3622 a         2823 c         2258 g         4345 t
ORIGIN      
        1 taaataacaa aataaattct cataattgta aaaaaaacta tctcattaaa agtttctatg
       61 ctatactggc tttgaaatgt gtctctttta atagcatata ttagcttatt tcttgctttc
      121 cccaaaaaac aagagatacc ccaaagttaa tttcgctcat gcgagacgct ttctccattt
      181 ctgacgtact ctcagaaatg gccccgttag aaaaggacac gagatctcca tctgttgatg
      241 gttctcatgt cctttttatg ttttaaagga gcggcgaaat caatcttgct aatccttctt
      301 tcatcttgat gattattcct cggttttcat acatttcttt tgtgagatgc gtagaaattt
      361 tcgagtcttc aaaaaaagct tttcttacac gtgaagccat tcgttcatca taaaccagtg
      421 tattgacctc aaagtccaag cgaaatgaac gaacatcgat atttgctgag ccaactgaaa
      481 caatgccacc atcgataatc atcgtctttg catgaataaa tccattctca tatgtttcga
      541 tccttgctcc atactcgatc agctctgcag cgaaagaata cgttgcccag taaacgagaa
      601 gatgatctgg tttgttaggt atttggatat gaacttttac gcctgaaaga agagccaact
      661 tcagtgcttc atgaatcgat ccatccggta tgtaataagg cgtttggatc aatatctccc
      721 gtttggctaa cgaaatcatt ttcaaataag tcattttgat ttgttcatgt tcggtatctg
      781 gtccgcttgt cacgatttga acagctatat caccagtagt gtgaatggac gggaaaaatt
      841 tgggagaata gcctacttct tttgtatgct gagaattcca gtccatcaaa aaacggtttt
      901 gcaaggtata caccgcttct ccgtaaatac gcaaatgatt gtctcgccaa taaccaaatt
      961 tctttaccaa tcctagatat tcatttccaa cattgaaccc acctgtatat ccgattttcc
     1021 catcaataac tacgatttta cgatgattgc gataattgat ccgaggatta atatacggaa
     1081 caaatagagg aaagaaaaat gcgacttctc cgcctgcttt tttcaaattc tgaaaaaagc
     1141 tggaagaaac ttgtgtggaa ccccatgcat caagaaggac tctgactttt acccctcgtc
     1201 ttgccgcctc tgtcaatgca tctcgaactt cttcacctaa tgcatcactg cgataaatat
     1261 aatactggaa gtggatatgg tcttctgcct gcttgatatc ttcaatcagc gcatcgaatt
     1321 tttcacgacc atctgtaaat aaagtgattt catttttagt cgtatagagc gtactttcaa
     1381 ataccgtgag catatagatc aattgtttca catctacttg tccagtaggc ggatgaggat
     1441 aaagccctcg catcaatgcc tgtttgtctt cttcaatttc caggttctta ccgattttcc
     1501 cttggatctt caaatcaaag attttctctt tggaaatccc tcttcctaaa aaaatatata
     1561 aaataaatcc tacaactgga atgaacatca aaacgagcag ccatgcccat gtttgagcag
     1621 tttcctttct ttctcgaaaa acgatgataa aggaaagcaa aatattcata atcaaaagga
     1681 tcgtaaaaaa attgtcaacg ataattttca cggcttcacc tcttcttcta ccaacttaag
     1741 aatacccaag ttgtttttta aagtaaaggg aaaaaagccg cttccttcta ttctcctagc
     1801 caataaaaaa agaaagctgg aacgaaactg tccaactttc tcattttatt tatattgtgg
     1861 tattagaatt tcaaaaaacg tctcatagaa atgatcgagc caagtgaacc aatgatcaca
     1921 ccaatgccga ccattaaaag attgattttc caaacgatat tttgtggacg aatcaatgaa
     1981 taatgggaac gtaataatga tggattgatc aattggaaaa accatgcata accaaatgtg
     2041 atgatcaaaa tcggaagaat cgcaccgatc agaccgatcc agccaccttc aaggaaaaac
     2101 ggccagcgaa tatatccatt ttttgcaccg actaaacgca tgatttgaat ttcacgctgt
     2161 ctagacaaaa tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac gaacagcagc
     2221 aatgccgctg caactaatcc ccatgtccga actgcaccag cgatttggaa aatccgatct
     2281 gaggaagctc caccgtaatc agaacgagaa acattcttca aatctgctgc ttcttctgct
     2341 acttgttttg tataagaagg atcagtagca cttactacat acacatcata taacgggtta
     2401 ttgtcacctt caaacaattt ccaggaatca cccatttcgt tttggatttt tttcaattcc
     2461 tgttctttgc ttgaaaattc aactttttta acatgatcta agtcattcaa ttctttttct
     2521 agctttttca tctcttcgtc ttttgtaccg atatctacga atacagatac atcaacgttt
     2581 ccttctatat cctgagctaa tttagttgca ttcatgataa ctgccatgaa tacaccgact
     2641 aaagtcaacg tgatcgttac cgcactgact gaagcaactg tcatccagcc gttacgtttc
     2701 aaactcttga tactttctaa taaatggcgg aaaaaggttc taatcatcgt atccatattc
     2761 cccttccgct tgatctcgaa taatgcggcc gttttcgata gcaattactc ggtgccgaat
     2821 cgtatttacg atcgtactgt tatgtgtagc catcacgaca gtagttcctt gggcattgat
     2881 ccgatctaat aatttcatga tttcccatga gttttctgga tccagatttc ctgtcggttc
     2941 atccgcgata agtactttag gcgtattcac gattgcccga gcaatagaga cacgttgctg
     3001 ttccccaccg gataattcgc taggaaagac tcgaactttg tgtttcaaac caacaaggtc
     3061 cagcacttcc attactcgtt ttttgatatc tcgaggtttt ctgccgataa cttgcatagc
     3121 gtacgcaaca ttttcatata ctgtttttct tggtaataat ttatagtctt ggaacaccac
     3181 gccgatttct ctgcgtagat aaggaacttc gcggtttttg attgctatta gatcatgacc
     3241 tgcgacgttc aaagttcctt ttgaagcttt ttcttctcga tacatcaatt tgatgaatgt
     3301 agatttaccg gctccagacg gaccaaccac atagacaaac tctccttgtt cgatgacaat
     3361 cgagagattg cggatagcgg tcgtaccatt tgagtacttc ttcattacat ccttcatttc
     3421 aatcatggcg ttctctccat atctttttca gtctttagct attatagcat ggcaatgttt
     3481 caacatgctt gcaaaatctt acaattttat ttcaatttaa caatgagttc ttattgtaga
     3541 tgcattttta aatagccgtc aataaataaa tcaaggtcac cgtccataac tgcttggaca
     3601 tttcctgtct ctacgtttgt ccgatggtct tttaccatag aatagggatg gaaaacataa
     3661 gaacggatct gtgaacccca gccgatttct ttttgttctc ctcgaagtgc agctgcttct
     3721 tgttctttct tttccacttc taattgataa agcttagctt ttaacatccc catcgcttgc
     3781 tcacggtttt tcaattgtga gcgttgtgcc tgactagcta ctactgttcc tgtaggcaag
     3841 tgagtgatcc gtacagcaga ttctgtttta ttgatgtgct gaccacctgc accacttgct
     3901 cggtacgtat cgattttcaa atcatctgga ttgatttcta tatcgatcgt ttcatcaagc
     3961 tctggcatca catctaccga acaaaaagaa gtatgacgtc gtttcgctga gtcaaaaggc
     4021 gaaatccgta caaggcgatg aacacctttt tctgatttta gataaccaaa agcgttatgt
     4081 cctttaatca gcagggtcac actcttgatc cctgcttcat cacctgcttg ataatctagt
     4141 gtttctactt gatagccatg ctgctctgcc caacgtgtat acatacgtaa aagcatgctg
     4201 ccccagtctt gggactccgt tccgccagcc cctggatgca attcgacgat cgcattgttt
     4261 cgatcataag gtccgtctaa cagcatagat aattcatacg tcttcatttt ttcatctaat
     4321 gcattgatac gtgtttctgc ttcttcttgc attgctgcat caggttcttc ttgcagcatc
     4381 tccaccatca attcgatttc ttctagttct tcggcaagag aatggaattg gtcataggtt
     4441 tctttattag cgttattttc gctgatcact ttttgtgcgg cttcgctgtc atcccaaaaa
     4501 ccaggttcag ccatttttgc ttctgcttcc gcgatgtctt cttccagctg atctaagtca
     4561 aagagacctc ctgaaacttg tgaccttttc ctgcatttct gtcaggagat ttcgaatttc
     4621 ggatatttcc atgttttttc cttctttcac tatacttttt tcattatatc tagtctaaca
     4681 gaatcagcaa ataacagaaa gaaggtgaga cgaattctta aaaaaagaat ttgtctcacc
     4741 aatcctgctg tcagaaatta gtttgcattt tttccatgac agttcttata ttttttcccg
     4801 cttccacaag gacaaggatc atttcttccg accttgttca catggattgg ctgttttttc
     4861 gcgctagtat tactattttc tttggcatct ccgtcttctg agtgagaagc tgttccttga
     4921 gaaacctgtt cacgctgtac gttttgacgg atctcagctt tcataaagat tcgagtgact
     4981 tcatactcga tggcgcctac catatcttca aacatcttat agccttctgt ttgatattcg
     5041 actaatggat tgttctgtcc ataagcacgt aagccgattg actgacgtaa ttgatccatt
     5101 gcatcaatat ggtctgtcca tttagagtcc acaacacgca agatcacgac tttttcaaat
     5161 tccaacagtt gctctggacc atttagttga gccgctttgg tattaaatac ttcttgtgct
     5221 cgttcgttta gatactcttt gatctcctga gatgtttttc cttcgatatc tgcaagagta
     5281 atactatctt catggacaag agtactgccg gcaaaatcga tgatactttc caaattccaa
     5341 ttttctttct ctaattgtgt atgcgcatct actacacgac tgattgtccg tttgaccatg
     5401 ttcattagtg gcttggataa gcttttttct tccatgatta cttcttgtcg ctgtttatag
     5461 attacttcac gttgttcacg catcacatca tcgtattgta agacgttttt acgtgtgtca
     5521 tagttatttc cttctacacg tttttgtgca gattctactt gtttcgtcag catcttgctt
     5581 tggataacag catcttcctc accgatcttc atccgatcta agaaagcttt gattcgttct
     5641 gaaccgaatc gtttcatcaa atcatcttcc aatgacaagt agaattgtga cataccaggg
     5701 tctccttgac gtcctgcacg tcctcgaagc tggttatcga tacgacgaga ttcatgtcgt
     5761 tctgtcccga taactgctag accaccaagt tctcttaccc caagtcctaa cttgatatct
     5821 gttccccgac cagccatgtt ggtagcaatc gtcacagcac ctttttgacc agcattcatg
     5881 atgatctctg cttctttaaa gtgatttttc gcattcagca cttcatgagg aatacgttct
     5941 tgattcagca tatctgataa taactcagat gtttcaaccg caacagttcc gactaacaca
     6001 ggttgacctt tgcggtagcg atctttgata tcttgcacga cagcgtggaa cttgctttgt
     6061 aatgttgggt aaagtaaatc tggacgatca tcacggataa ccggacgatt tgtcggaatc
     6121 tggtatactt ggatgttata aatttctctg aattcttctt cttctgtttt agctgtacca
     6181 gtcattcccg caagtttctt gtacatacgg aaatagttct ggaaagtgat cgtcgccatg
     6241 gttttcgttt cgtcttcgat ctctacgcct tctttagctt cgattgcttg gtgaagtcca
     6301 tctgaataac ggcgtccatc catgatccga ccagtgaact ggtcaacaat cagtactttt
     6361 ccgtcttgga caacataatc gatatctaaa agcatgatat agttggctct caatgcttga
     6421 tccaagtgat gggtcaatgc agtattttca atgtcgtata agttatccaa accgaatgtt
     6481 tcttctgctt tttcgatccc tgcttcggtt aaactgattg tttttgattg tacatcgatt
     6541 ttataatctt cttcttcttt caaacgtttc acgaagttgt ctgctcttgt atataaagct
     6601 gtggattttt ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc aatcaagatt
     6661 gagtcaactt catcgacaat ggcataattc agcggacgtt gtaccatttg atggcggtaa
     6721 acgaccatat tgtctctcaa gtagtcaaat cctaattcat tattcgtact ataagtgata
     6781 tcacaattat aagcctcgcg tttttcatcc gaagattttg agttgatatt caaccccact
     6841 gttaatccta agaaattata aagttcgccc atttccgtcg agtcacgggt tgctaaatat
     6901 tcattgacgg taactacgtg taccccttcg ccagtcagag catttagata tactggcatc
     6961 gttgcggtca aggttttacc ttcaccagta cgcatttcag ggatatttcc atcgtgaagc
     7021 acgattccac ccatcaattg tacgtggtat ggatataacc ccaatacacg ttttgctgct
     7081 tctcggacga ctgcaaaagc ttccggaagt aattgatcta atgtttctcc tttttggtat
     7141 ctgcccttga attcagcagt ttttgcctgt aattgttcat cagttaatgc tgccatttgg
     7201 tcagcgtatg cttcaacttt ttttgcaatg ctatctaaac gttttagttc tttcttatcg
     7261 ttttcgataa gtgaacgaat caaattggcc atgcgttgtg taatctcctt ttccttctgt
     7321 gtattatcca gactatgtag actttacttt tcattttatc attagttata gataattgaa
     7381 atatcttaag cgtattaaag taagaacttc ataatttatt catatttcat ctaaaagagg
     7441 aggaaactca aaaaaacttg cggcagctgt cagtagctgc tgcaagtttt cgttgttatt
     7501 taagtaaggc tgatcagtct gtttcaatca gtccgtattt tccatcttta cggcggtaaa
     7561 cgatacttgt accatttgtt tctgcatctt caaaaataaa gaagttatgt cccagcatgt
     7621 tcatttgtaa gactgcttct tcactatcca ttggtttcaa tgaaaggcgt ttcgtacgta
     7681 cgatatctaa ttcagaatca ttttcgtttt cgtcctcttc attgaataaa gcagcagcat
     7741 ctgcagtatt catgcccgtt tcacgggatt tacgattaat ttttgttttg aatttgcgga
     7801 tttggcgttc caatttatca acaaccaaat cgatacttgc gtataaatct ggagaagttt
     7861 cttcggcacg taatacaaga taaggtaatg ggatcgttac ttccactttt gctgtttttt
     7921 ctgtataaac ttttaagttc acatgcgccg ttgcatcggg tgcgtcactg aaatagcgtt
     7981 ctagtttgcc tacttttttc tcaacgtagt cgcgaatcgc ttcagttact tcgatatttt
     8041 cgccgcgtac attatatcta aacataaaaa ttaccccttt cgtctaccac aatcaagaag
     8101 gagaaggacc actctttccc tcttcttaat tctattatat cgaactctag gttatttgca
     8161 aattaaatcg ctatcaaatt tccacatcta attcatctcg ccaaagaaat ggttcggatg
     8221 attttcggct gtgcgacctg caaacaatct gctgcgtgga aaagagtctg tcctgttgta
     8281 tagacatcat caataatcaa tattttttta ttttgtatct tagaaaaggt gactgcaagt
     8341 tcaaaaggtt gaggcatctc caatctttcc tttcgcgtgt tttttgcttg agccagcaga
     8401 tgcttctctc ttttgagtag catctgaaaa ggaattcttg ctgctttcaa catctcgctt
     8461 acttgattga atcctctttc tgttcgcctt ttttcagata aaggtattgg acaatagatg
     8521 tatgtcgggt attctttttt tatctgcgtc catttatatg caaaggtgcc tgctagtcgg
     8581 atatctccca taaacttgta ttgattgagc caggccttca tattttcatc gtacacaaaa
     8641 aaggcacggt gctgaaaatc ataggtcgga tatttcttct tccattcttg acagtcactg
     8701 cagtaaacgg gaatagactt tactttcttt ttcgtactct ctagattgaa atggattggt
     8761 ttttgacagc cagaacagcc tcttgtttcc aataaatgaa attgtgcgcg acaaactgaa
     8821 caagtctgag gtaatctgta tttcgaaaca ccaataatct cgctgaatgt taaattttgg
     8881 aagatatctt tactgcagca actgcacctc acagcatctt cttcccttct tcattcattt
     8941 ctttgatttc ctgacaagct tttcgaatag ctgcagtttt ttcttcatag aaaaagtata
     9001 cttgactgtt cgtataaggc cctttacgat cggctcttcc tgcgatttgt accaaagaag
     9061 atttagtaaa aaccggatga tccgctccta aaacgataac ggatattctt tcaaatgtga
     9121 ctccacgttc aaggatcatc gtggttaaca gtatatcata ttttgcttct cgcattttct
     9181 ggactttttc cgcccgctga ttgtctcttg aagaaacatc tgtgatctca tatctcggaa
     9241 gagcctcttg aagtgtggct ttcaaacgat gcatcatcgt gatacttgga caaaataaaa
     9301 ggatatggtt ttctttaatt aattgatgaa tgatccgcac caaagatttt atcttcttct
     9361 tgttcttgca tttttgtcgc caattattca tgaaaaccaa gacgggttca ggaagcaaac
     9421 gccgatgaaa gcgtaaagca agtttgtgga tgtcaaacgt ctgcttgatt tcctttagta
     9481 actttttatc aggtgtggca cttaagtaga tgaatcgtcc gtctggttta atcgctgttt
     9541 gcacagcatg agctaatcct gcatccccgt tataaggaaa cgcatcgatc tcatcgatca
     9601 ctattaaatc aaaggaccga taaaaacggt atagctgatg aatcgtacag ataacaaaag
     9661 aagtaaaggt ataagccgtt gctgtattcc catggagcaa cgtgattttt tcttctggaa
     9721 aaacataacg atagcgtaaa aacaattcat tacaaacatc gactcgcggt gtacaaatcg
     9781 ctactcttcc tccagctttt aaagtttcca ctaaaatagg ataaagcatc tctgttttcc
     9841 ctgcacctgt tacagcccaa ataagatgat gtcttttttc tgaatggcaa agtattctct
     9901 ttgagatttc cgtttgtttt tcagtaagct gtcctgtcca aaaaaagacg acttctcgag
     9961 gttttgcttc aggctgatca aacaaatata aatcttgttg agtgtcgcat cgccctaaat
    10021 atagacaatg tgtacaaaaa taaccgtaat ctgcaagctg tccatcaatg acggacgtcc
    10081 tcattccaca gcgatgacaa agaatccagt tgcccaaacg ttccactgct gcgatcttct
    10141 cataatttgg taaatttacc tcctcacagt ctattgcttc aatatctctc gtcagaagcc
    10201 ttctacccca aaaatgattc atattcttcg ccctaaaaaa atatacgaaa aaacaagcga
    10261 tctatttttt ggatcgcttg ttcgatagtt atactgttct atttattcac ctatgagcgt
    10321 gattgcttga tctaacactt tttcgccatt catcatacca taatcagcca tattgatgac
    10381 atctaaagga atgccttttg gttccagacg tttctcaaaa tcagctttca taaagcgaac
    10441 ttgtgggcct aataacaaca catccacgtc tttatttgca aggttgttat ctgcgtcaga
    10501 agcggacaca gcgaaaatat ccgagtcgat tccttttgct tcagcagctt tttgcatttt
    10561 tgttactagt aaacttgtgc tcataccagc cgaacataca agcatgattg tttttttagc
    10621 catacttttc tctccttttt aaaacaaact atgcaaacgt tttcttcgcg tctgttactc
    10681 tattatataa aaagaaagaa atttgtcaac gttttctaaa cgcggtttat ttaatcaaat
    10741 agccaactag atttcactaa tttattgaac acgacatatt ctttgtccaa attgatgaca
    10801 actacttcat agcctgcatt tatccagcca taggcacctt tttcagggtg tttataatca
    10861 tttgcccacc aatctgtact ttcccttgct gttttgggaa gtccgttact gggaaacagc
    10921 agttcgtcaa attgggtaaa ggtcaacgtt acttgagagc cgccattgtt cttcaaataa
    10981 tgcgtaattg catcgtactt tttcattcgt tcctgcatcc ccagatccac ctgcctttgt
    11041 ctttattatc gcatactaca gcaattgcgc aaccgttaca gtgttgaaac aacctagaac
    11101 tttatttata ctgaataaag aaaatgaaaa aaagaggtga caagatgatc gagcgctatc
    11161 gtacaataaa agaaaatgga caaagtgaga ttgagatcaa aaaatcacgc tttatctgtt
    11221 ctcttagacg aatagaatca gaggaccaag caaaaagttt cattcaagaa ataaaaaaag
    11281 aacattggaa agcgaaccat aactgcagcg ccttcatcat tggagaaaaa aatgaaatcc
    11341 agcgaagcag cgatgatgga gaaccaagtg gaacggcggg cgtaccgatg ttggaagtcc
    11401 tcaaaaaaaa tcaactgatc aatgtagctg cagtagtcac tcgatatttt ggaggaacga
    11461 aactcggcac aggcggatta atacgagcat acactcatgc ggtctctcaa gcattgtctg
    11521 acattggaat cgtagaaggt accttgcaac aggagatctc tgtgatgatc acgtatccgc
    11581 aactaggaaa attacagaat tatcttgaac tccaacattt ttttataaaa gaaatccaat
    11641 atactgatca agttagcgtc atctgtgctg tagatgaaaa aagtgctgac cactttaaag
    11701 aatccatcat tgagctgctg aacggacaag tccaatttga agagggttct gtttcttatc
    11761 acgagcaact gataactgat catatataaa aacaggtgat tctgattctc ttttatttct
    11821 aataaggaat cagggatcac ctgtttttta tttttccaaa taaaactcaa aacgatttcc
    11881 tacgtattgc gtacgcacat actcaaacgg cgtaccgtct tcaaaataag aaatctggcg
    11941 caaacgaaga actgcatctc ctcgttttac ctctaaatag tcagcaattt tctcagaagc
    12001 caacacagca gtaatcgttt gattcgctgg accgatcttt ttcccgcctt tttcttctag
    12061 cgtgcgatac aacgattcag taatctctgc tttgctgtag ccatcgatca gcttttgtgg
    12121 aacactggca acttcaaaac aaatagggac atcatctgcg aaacgaatac gttccattcg
    12181 caagatcgtg tcatctttac tcaagtttag tttttccatt tcacttgagc ttggcgaagt
    12241 caagaaataa gagattgtcc gactagacgg tactcgattt tgtgattcca taatttctgt
    12301 aaaacttgtt gcaccagaca ttttttcttg tactttttct cgcgcaacat acgtacccga
    12361 accgatcttt ctttctaaga ttccttcatc tgccaagttt tgaattgctt gtctcaacgt
    12421 catacggctg acattgaatt tcactgctaa ttctctttct gaaggaagac ggtctccgac
    12481 tttccatata ccctgttcga tttcattttt taattggtca tgaatctgta tatatacagg
    12541 taattgattc gacataacgt acacccgtcc tttcgtttct tctattataa ctggtatata
    12601 cctgattcac aactgaaaaa gataaaggtt tctatccatc aacagaggat ttagacacac
    12661 atcgttctcc ctactcctat tgtcagaaac aattttttag atatgttcaa cctagaaaat
    12721 aacaaaagac cctaaaatcg atgaaaatgc cgacttatgg atcttttgtt attattagcg
    12781 gtttttagcc atccattttt ttgtatgatt cgttgggcgt tttgatttta cctggaccgc
    12841 ttttccgcct tttttcggaa tattcacttc aatattgtat ttttctgatt tgaccaaatc
    12901 atataacttt tcagctgctt cgtctacttt ttctttccat ttcatgtgga tccctccaat
    12961 tcttgtagtt aacttaactc tatcttaatt cagcagaatc ttcaaggaac aaggtagcaa
    13021 agcaaaaaaa agagctctcg aactaacg
//
LOCUS       DAJSKS010000074        13013 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJSKS010000074 DAJSKS010000000
VERSION     DAJSKS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.076"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..608
                     /locus_tag="ON396_001768"
     CDS             <1..608
                     /locus_tag="ON396_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113853.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
     gene            628..1656
                     /locus_tag="ON396_001769"
     CDS             628..1656
                     /locus_tag="ON396_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCR3113854.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1653..2411
                     /locus_tag="ON396_001770"
     CDS             1653..2411
                     /locus_tag="ON396_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR3113855.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2497..3237)
                     /locus_tag="ON396_001771"
     CDS             complement(2497..3237)
                     /locus_tag="ON396_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3113856.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3298..4551
                     /locus_tag="ON396_001772"
     CDS             3298..4551
                     /locus_tag="ON396_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR3113857.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4852..5100
                     /locus_tag="ON396_001773"
                     /pseudo
     CDS             4852..5100
                     /locus_tag="ON396_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5222..5548
                     /locus_tag="ON396_001774"
     CDS             5222..5548
                     /locus_tag="ON396_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCR3113858.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5616..6329
                     /locus_tag="ON396_001775"
     CDS             5616..6329
                     /locus_tag="ON396_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR3113859.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6639..7491
                     /locus_tag="ON396_001776"
                     /pseudo
     CDS             6639..7491
                     /locus_tag="ON396_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            7612..9477
                     /locus_tag="ON396_001777"
     CDS             7612..9477
                     /locus_tag="ON396_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3113860.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9525..10934
                     /locus_tag="ON396_001778"
     CDS             9525..10934
                     /locus_tag="ON396_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCR3113861.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            11042..11743
                     /locus_tag="ON396_001779"
     CDS             11042..11743
                     /locus_tag="ON396_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3113862.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11902..12381
                     /locus_tag="ON396_001780"
                     /pseudo
     CDS             11902..12381
                     /locus_tag="ON396_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         4377 a         2181 c         2746 g         3709 t
ORIGIN      
        1 cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac caagcaggag
       61 aagctgctgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt gggaatcaca
      121 atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct gctacgaaga
      181 ttgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgattgt ggtcagatca
      241 aagcaactgg gttgacgcaa gaagcatttg gccaaatgga tatacaggaa aaattatgga
      301 tttatgatca gcgagaaaca aaaagtccat ggatcttaac gataaaatta tctcagcctt
      361 tcacaaccga tgcaggattt tctatgcagg atgtcctata tttcaagaaa gctgaggagt
      421 atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca acgaatgtgc
      481 aaacagatta ttcagaatat ttaaataatg gtcgggcgtt taaacttgtc ttaaccaaag
      541 aaaatcagat agctggtaat tactcagcag agatgacatg gacattagca gatgcgccga
      601 cagaataaag atcaaggaga atgattgatg aatcgaatat ggaataaaag tcatcgaaac
      661 aactgctctt tacttctgat ccttctcctt ggattcatcc tttggcagac acctgtccat
      721 gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat tgataagaac
      781 aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc ctacacggtc
      841 tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc tacgacgaat
      901 tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac attaagttat
      961 gatatgaaaa acttagcaaa aacagtggga gaaataactg tccctcctca aagtgcggtc
     1021 actgagacac tttcactgaa agctccagaa aaagagctgg ctggtgtggt cgcaggagga
     1081 atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat
     1141 cagttttctc aagtgaaagc aatcttgatt cgaaatagcg aaaaagagtc ttccccaaat
     1201 ttgaaattaa aaaaaactta tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat
     1261 cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga aatctacaaa
     1321 aaagaaacaa aagagcctta tctttcaata aaaaaagaaa aaatagatgt agcacccaat
     1381 acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg acattatcaa
     1441 gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga ttttgaaata
     1501 actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa tcagccagaa
     1561 acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt atttgctgga
     1621 ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg
     1681 ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat aagagctcag
     1741 ccactacaga aggaacgatt tctgtaacaa aaccagatgc cgcaaatcct gaagacgttg
     1801 ttccagttcc tcctgttgat ccaaatgatc ccgatacgcc gtttacacct aacgaaccaa
     1861 atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat tttggaacaa
     1921 tcaagttagg tacagcaaaa acagtatatg ctgcattaca aaacggaacg gacagtacag
     1981 ggacagcaac acaagtacag aatttcgtac aagtaacgga taaaagcgga atttatgccg
     2041 gttggaaact atcagtcaaa cgaacagaat ttattggtac aacatctgat acgaataaac
     2101 taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca actaatagca
     2161 gtggaacgcc atctactatt tctggtactg gcgctgaagg aattgagatc ccagcagatc
     2221 aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg atttattcat
     2281 taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct gtatccaatt
     2341 atgccgtaga caactacgtt tcaacattca catggtcgtt aacagatgct cctacagaag
     2401 ggaaagcgta aaatcaaatc aatgacttca gggagtggag ccgattcaag gccaactccc
     2461 tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag ttttttggaa
     2521 gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta aggagtcacc
     2581 attgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg ataagttgat
     2641 acagtaagat tttaatagta aaaagtaagc atacttttcc aactctgtct tcaattgttt
     2701 taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt ggtttttcaa
     2761 acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac ttccaccttt
     2821 ttgatcaata aagattttat cgacatttac gatttgctgt tctttccaat tgatctgttc
     2881 tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc caatcggaag
     2941 gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt
     3001 actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga gtgattgctt
     3061 caagtcgata tcgataaaga aaacatcgtg attatcaata gtacccaact ggatcaactg
     3121 attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat tgaccaattc
     3181 tttgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact ttttcatatg
     3241 caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag gaataagatg
     3301 gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg tatctattta
     3361 ttcttttcga aacaactgct agtaaaaaac agctttggtt ttatagcttt gatggtggtg
     3421 acacttttat ttcctatccc taaagtgagc tttccttacc tagttggctt attattgatg
     3481 tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag taatcttttt
     3541 tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta tttaggaaag
     3601 agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt ctatctatta
     3661 gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta tccaactgaa
     3721 cactattttg gaggtatttg tattttatta agctggggcg tgatttactt ggtattggga
     3781 agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat agtcatcagt
     3841 tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga aagacaacat
     3901 caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct agaggaattc
     3961 aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga aaataaaaaa
     4021 gaaacgttgg aatttttaga aatcttagaa gatcattctg cagctatttt tgaaaaaccg
     4081 caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt tttaaaattt
     4141 ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa
     4201 acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat ttctaacgca
     4261 ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga agaaaaagag
     4321 ggattacgtt ttagtatagc gaacaaggca aatccgacga atttgacgct atcggagatg
     4381 gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa
     4441 atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat
     4501 ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc
     4561 aaaggataag cttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa
     4621 agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca
     4681 aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt
     4741 agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc
     4801 agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagaa aatccctacg
     4861 atcacacaaa aaatgctgac ccagcagtta agagagttag aacaagatgg cattgtccat
     4921 cgaaaagtct ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa
     4981 tcattaagag agattctagt agcgatgtct gtttggggag aagaacgtat cgaaaaacaa
     5041 aaagcctcag gggaagaagt cacattactg catacacatg gtggctacct aaattactga
     5101 tttctatgac atttatttta aataaaagaa aacaggagtt tttttaaaag catcatctat
     5161 tttttatgta aaaaaatggt atgattaatc aagtaagaaa tgattggaag aaggcaagca
     5221 aatgaatagc atggaaaaag ttaaccagtt tagagatgaa cgtaattggc gacaatttca
     5281 taacgaaaaa gatttggcta tttctatttc tttggaagca tctgaattgt tagaactttt
     5341 tcaatggaaa acgccggaag aagtaaaaga aacacaatta gaacgaatca aagaagaact
     5401 ggcggatgta ttgatctatt catacatgat ggcagacaac cttcagctgg atctagatga
     5461 gatcattgaa gaaaaattga tcaaaaataa tttgaaatat cctgtagaaa aaagcgcagg
     5521 tgtacgaaaa aaatacacag agttttagaa atgtgtcatt cggctgttgg aaagagcaaa
     5581 agaaaaacag taaaatgagt ctggagggag agaaaatgaa aaaaaaagga aaagcaaaaa
     5641 gatggctagt caatatcctc ttgttcctct tgttattagt aggactagct ttgattttca
     5701 atgaacagat caaggattat cttgtcagag agacaggaga taaatatgct atagcgaatg
     5761 taacaaaaga agatctgaaa aagaataacg ataaagatgt cagttttgac tttgatgcgg
     5821 tagaaccaat gacgacagaa ggggtcatga ggtcgcagat gagaggaaca gatctaccag
     5881 tgattgctag tatcgcagtc ccttcagttt caatcaattt accgattttc aaaggcttgg
     5941 ataatacgtc actactttac ggagcaggaa cattatctcc agatcaagaa atgggaaaag
     6001 gaaattatgc cttagcaagc catcgtgcaa cgaaccctga attgttgttt acaccgctgg
     6061 aaaatttaga gatgggtgcc aagatctatt tgacagattt agaaaatgtg tatacataca
     6121 aaacattttt taaagaaaaa gttgctccta cagataccca attactgaat gaagtcgagg
     6181 aaaaagaaat tgtcacacta attacttgtg gcgacatgga cgccgtcacg cgactagttg
     6241 tacaaggaga actggaaagt gttacaccga taaaagatgc gacagatgat atgagatctg
     6301 ctttcaatct agagacaaaa acattttagg atactgggac ataagtcgtt acagattaaa
     6361 aacagccaaa ggatatgaat cgataaggaa aaaccaattt gctgaaaaaa agaggaacgc
     6421 aaagaggtgc atctctgatt tggttgtttt ctagatagac ttgtgttcca gtttttttat
     6481 tttttatatg aaaaggcttg cataaataaa aattcgatgg tatgatgatt ttgaataaga
     6541 aatggattgt tactgtacgg caggcaaaac cagaattcag ctaagaaaca gtaggcgaat
     6601 tctaggtttt tttatttttc ttgagagggc gaagcaaaat gaaaattgat aaaattttga
     6661 ataacaatgt cgtcatttcg aaaaatggat tcggggaaga agtcgtttgt atgggaaaag
     6721 gactagcttt tcaaaaaaaa gattggggac gagatttcgc cagaagcggt ccaaaaagaa
     6781 tttattttaa gagattcttt tgctaaaaac caattccagc agctgatggc tgatattccg
     6841 gcagaggaga tcgaatgggt caaacaagta gtcgaactgg ctgaagataa actaaaagtt
     6901 gaattttcgc ctaatattta tctgacattg accgatcatc ttcattatgc gatcacacga
     6961 gcaaaagaaa atatccaact gcctaatcca ttattatttg aaacaaaaaa attttatccg
     7021 aaagaatatc aagcagcgaa agaggctctt ttgttcgtga aacaaaagac gggtgtggaa
     7081 ctttctgagg atgaagccgg ttttattgcc tttcattttg tgaatagcca acaaggaaac
     7141 gaagacatgc aagtgacgat gaccgctacc acaatggtaa gagatatact gagtatcatc
     7201 agtaaattct tcggcatcgt gttcgatgaa gaatctttga attatcagcg tatcattact
     7261 catctgcagt tttttgccca gcgttatctc aaaggggaag gatctgacga gcaagatgaa
     7321 tttctctacg cattagttca aggaaagtat ccaaaagcgt ttcattgtgt cgaacggatc
     7381 aatgagtatc ttcgacaaac gcaaaaacag gaaatgggga tcgcagagca gatttattta
     7441 acgattcata ttcaaagagt agtaagtgag aaaaaaagca tccaatcata aggattgtta
     7501 ctgtatggca ggcaaaacct ggattgaaaa aataggttca cgaatgtttg tgactgtttt
     7561 tttcaatctg ggtttttatt ttttaaataa aaaagaaaga aggaaaaaac aatggataat
     7621 caagcagttg gtcgtcgtgt atgggaagcg gtaggaggac aaaagaatgt caaaagttta
     7681 gtacattgtg ctacgcggtt gcgattcaga ttaaaagatg aatctttagc agatacgcaa
     7741 aagttaaaag aagacccgga cgtgatccaa gtcgtacaaa gtggcggtca gtatcaagta
     7801 gtgattggca gtaatgtagc agatgtttat caatcgatcg tggatgagga aggtcttgca
     7861 gtttcggagg atggaaaaga agagtccaaa aatattttga atcgtctgat cgatattatc
     7921 tctagtatat ttacaccttt tctaggggcg atggcagccg ccggggtatt gaaaggattc
     7981 ttatcattgg caacggtgat gggctggctg acagctgata gcggtactta tcaaatattg
     8041 tttgctgcag cagatggtgt ctttaccttc ttacctgtca tgcttgcttt tacagcagct
     8101 aaaaagttca aagccaacca gtttttagca gtagcaattg ctatggcact tgtgtatcca
     8161 gctatcacag cagttgctgg tgcgggagaa gctatcagtt tctttgggat cccagtgatt
     8221 ctttcaccat ctggttatac ttcttctgtc ataccaatca ttctagccgt atgggttcaa
     8281 agtaaattag agccattcgt aaaaaaagtg attccgcaat ttctgcaaat gattttagtt
     8341 ccgcttgtcg ttttagtagt gatggttcca ttaacatttc ttgctttagg accaatcggg
     8401 acggttgccg gtaatgcttt aggcggtctc ttcaactcga tttatggatt tagtccaatc
     8461 gtagcaggtc taatcatggg aagtttatgg caagtattcg tgatgttcgg catgcattgg
     8521 ggattcgtac cgatcatgtt tttgaatatc gaacaatatg ggtttgatgt attgatgcca
     8581 atgcttcttc cagccatctt ggcacaaggt ggggctgctt tagctgtagc attgcgaaca
     8641 aaagatacga aacttcgagc attagggatt tcttctacag tgacttcgtt atttggaatc
     8701 actgaaccaa ctgtgtatgg tgtgacatta cctttgaaga aaccattcat tgccgcttgt
     8761 atttctggag gtattggtgg agctattatc ggattttccg gagtaaaagc tttctccagc
     8821 agtttggtca gcttattgac gattccgact ttcattaata cggtggatgg tgtagagtca
     8881 aatgtgacag tagcagtaat agctacagga attgcttttg ttcttgcatt cgtaggtaca
     8941 ttgattttag gatttgacga acaaacacaa gacaatcaat tagaaaacaa acatgcgaat
     9001 gcaggagaac cgattacttc agcacggcat accttaaaaa gtcctttaac gggtaaagta
     9061 ctgccattga gtgaagtacc agaccaagtt ttttcttctg gcgtgatggg aaaaggtatc
     9121 gctattgatc cagaagtagg agaactagtt gcacctgcag acggagagat cacgactatt
     9181 tttccaactg gacatgctgt agggatcaca acgactgacg gagcggagat tttgattcat
     9241 atcgggatgg atacagtaga attaaatggg aatggctttg agatcttagt caaacaaggg
     9301 gatctcgtca aagcaggaga tttattgatt cgttttgaca tcgaagcaat tagagcagct
     9361 ggctatagcg taatcactcc agttgtcatt acgaatactg atgcatttgc cgatatactt
     9421 gaattagacc aaaaagagat tattgcaaac gaagatgttc tggcaattgt aaaataagga
     9481 ggaaatcagg atgcaatcac gtttttcaga aaaattttta tggggtggag caactgctgc
     9541 caaatcaatt agaaggggct tatgatcgag acggaaaagg actatccgtt gcagatgcta
     9601 tgcctggagg taagcagcgc tttgcgatta tcggcagtga agaatttgac tggacgattg
     9661 atcaagaaaa atatatttac ccgaatcatc gaggaatcga tcattacgat cgtttcaaag
     9721 aagatcttgc cttgtttgcg gaaatgggat tcaaatgtta tcgtttttcg attgcctgga
     9781 cacgaatttt tccaaatgga gacgaaggta ctcctaacga agcgggatta gaattttatg
     9841 atcagctgat tgatgagtgc ttaaaatatg atattgaacc tgtgatcacg atttctcatt
     9901 acgaaatgcc tcttcatttg gcaaaagaat acggtggctg gaaaaatcga aaactgatcg
     9961 acttctatga acgatttgct cagacagtat tagagagata cagcagtaaa gtcaaatatt
    10021 ggatgacttt caatgaaatc aattcggcct ttcactttcc agctttaagt caaggattag
    10081 taaaaagcaa tggagcagga gagtaccaaa atattttcca agcatggcat aatcaattcg
    10141 ttgcaagtag caaagccgta aaaatcggcc acgaattgcg ttcggatatc caaatcggct
    10201 gcatgattat ctatgcaaca acttacagca tcgatgccaa tccagtcaat caggcagcaa
    10261 caatgatcca aaaccaagaa ttcaattttt tctgcacgga tgtccaagta cgtggagaat
    10321 accctgcata cacagcgcgt acccataaaa aatatggagt cgatccggaa aaattagagc
    10381 aaacggaaaa agacttccag ttgctaaaag aatatccagt ggactacatc ggatttagtt
    10441 attatatgtc tacggcaatc aatgagactg atccacaagc agcaacttca gaagggaact
    10501 tgctgggagg agtaaaaaat ccgtttcttg aggcaagtga atggggctgg cagatcgatc
    10561 cagaagggct gagaatcgca ttgaatgaat tatacaatcg ttaccagaaa ccattattca
    10621 ttgttgaaaa cggtcttgga gccattgatc atatggaaga ggatggcacg attattgatg
    10681 attatcggat cgattattta cgtcgtcata tcgaagcgat ggctaatgca gtagcagacg
    10741 gtgtagattt gatgggctat acgccttggg gatgtatcga tcttgtcagt gcatcaacag
    10801 gagaaatgag taaacgttac ggctttatct atgtggatct agacgatgaa ggaaatggga
    10861 cactgaatcg atcaaagaaa aaatcttttg attggtataa agaagtaatt gcctcaaatg
    10921 gcgtaaactt gtaattaaag cagctgaatg aaaaagaaca cacttctcac atgtgatttg
    10981 tgttcttttt ttatttttta tgtgctattc ttttagtaat agtaaagcaa agggtgacgt
    11041 tatgaaaagg acacgtgtac tatacttgga agtcgcagat aaaattaaag aagatatttt
    11101 ctcaggaaaa tatccagtcg gttcgatgtt gccgacagaa agtgaattag aagaactgtt
    11161 tcaagtcagc aaaatcactg tccgaaaggc aatcgaactg ctggcatcag atgaatatgt
    11221 cgcaaagaaa agtggtcgtg ggacgacggt tctaagcaat cgtccttaca atcgattatc
    11281 caaagcggca agctttacgc aaatcttgga gaactcccga tatgaagtcc ggaaagaaac
    11341 gctggatttg caattggtcc atataaagaa ggaagatcct ttgttttcct ttttcggtga
    11401 gcaagctgtt tgttttaagc gcttatatta tttgaacaat cagccttata tttattttgt
    11461 tcattatctt ccacaagaaa tgatgcaatt cagcagagaa gaatttggca aagaatcttt
    11521 gtatcgtttg ctgacgaaaa aaggatatac tattgactca ttttctgatg atttctcggc
    11581 agtattttta acagaagaag aacaaggaat tctgaaaaca gacgcacaga tcggcatcaa
    11641 acggatacgg aaatcgtcta cgcaaattgg ggaaatgatt gaatattcgg aggcggtcta
    11701 ccatacggag ctgcatcctt accatattga ttatgaaaca taaataaaaa aagaggttgt
    11761 gaaaaagctg ccctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt
    11821 ttcgtttggt taggcttatt accgcagata cacggctgtt tctggattac catttattct
    11881 ggataagggg gtgtggcacg attttcagaa ggaatctatg aaccaatcac tgtattgatt
    11941 ttagcttcga tggtaacccc attcggcttg acgatcgctt atttcttagg gaaaatcatt
    12001 cgtaaaaata tactcaatcg tcaagaaatc gatacattga agaccgcttt tcctatgggg
    12061 atctgtcaga tcacagaggg atgtttccct atcgtattga acgacttggc acgcaatgtc
    12121 atagcaacgg gagtcggtgg gacagtaggt ggcggattaa gcatgttttg gggcgcagat
    12181 agccgtattc cggcatcggg aatgtttgca gtcgctacga tgaccagacc atgggcattc
    12241 attggtgcat tgttagctgg ttcttttgtc acaggaataa ccattttatt attgaaaaaa
    12301 ccagtagatc cgaatgcaga aatcatccaa gaagaaaaag aggaagaaga catatcttgg
    12361 gatgatttga caattagtta ggagaggcag aatggattac aagaaaaaag tacaatagat
    12421 gtaccttgag acaggaatgg tatgaaagaa tcagagttag aaagaatcga atatacagat
    12481 tttggtttag gagcgatcga aactgtccag agcatcggca ccctccttga aatgacgatg
    12541 aaggaaaaat gagaaccttt cgttgtcgaa agggtctggt ataccttatg tcgaaggaaa
    12601 ggaaacaaaa aaactttcct gtactattcc aaaaaggaaa gaaagttatt atacagtaaa
    12661 aaaagaaatt attcttcatg ctagagatca gtacacgttc gagcccaata tcaaacacta
    12721 gttccaggca ggcgcaacag gtgcggttat cactgaattt tcttccagca gtgacgattc
    12781 aagtgatatt tttacaaatc cagctattta tcgttaacaa aaagattttt aggagaagct
    12841 gatgaacaaa aatgatcaaa ttttgatcgt ttagttcgtc ggctttttta tttagagaaa
    12901 catcatttat cagtgaaaca taacacgaga acaaaatgat tttttcgtta ataaaagaaa
    12961 caagtatttg ttgtttaaaa taatacacgt tattggcata taggacgaaa tcc
//
LOCUS       DAJSKS010000075        12772 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJSKS010000075 DAJSKS010000000
VERSION     DAJSKS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.077"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            52..3579
                     /locus_tag="ON396_001781"
     CDS             52..3579
                     /locus_tag="ON396_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HCR3113863.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3576..7298
                     /gene="addA"
                     /locus_tag="ON396_001782"
     CDS             3576..7298
                     /gene="addA"
                     /locus_tag="ON396_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCR3113864.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7367..7708
                     /locus_tag="ON396_001783"
     CDS             7367..7708
                     /locus_tag="ON396_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3113865.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8017..9060
                     /gene="pheS"
                     /locus_tag="ON396_001784"
     CDS             8017..9060
                     /gene="pheS"
                     /locus_tag="ON396_001784"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR3113866.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9065..11485
                     /locus_tag="ON396_001785"
     CDS             9065..11485
                     /locus_tag="ON396_001785"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR3113867.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11655..12665)
                     /gene="trpS"
                     /locus_tag="ON396_001786"
     CDS             complement(11655..12665)
                     /gene="trpS"
                     /locus_tag="ON396_001786"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR3113868.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4319 a         2232 c         2716 g         3505 t
ORIGIN      
        1 ttttttgata taataaagga gcaccgtatc atgtgttgaa ggaggaaaag catgtcttta
       61 caatttattc ttggaaacgg gaaaaaagat caccgtcaag tcttgatcga cgaagcatcc
      121 gcttggctcg accaggatgc aagtaaccaa gtatttttcc ttgttcccaa ctacagcaaa
      181 ttcgaacagg aacaagaaat tttagcggag atgcgaagaa gaagcggaaa aaaaactttc
      241 agcacaacaa acgttcaagt atttagtttt tatcgtctgg cttggtattt gctgcaacag
      301 acaactcttc ttacaggaaa tgaattatca gaatcaggat ctgcaatgat cttgcgacag
      361 attttagaaa aaaatgagga agaattaaca atctttcgcg gagaaatcaa taaaagaggg
      421 tttattcgtc aattacaaga actctacagt gaattgcgta caggaaatat tcaaccagaa
      481 gacctgatgc ttttatttgg tgattcacct aatgcagaag accagcaact taaaatgaaa
      541 gacttgaagc tgattttttc agcttatgac agcgaattga ctcaacgtgc actaacaaat
      601 gaagatgcac tatctatact agcaaattat atgcagacac aagatttcaa caacgtcaaa
      661 tttattgtca gtggtttttc tcgaatcaat ggtcaagagt atcaattact gcaggcaatg
      721 atggaaaagg ggcaattggt tgtcgattta ttgctggatc gtccttatac agcggatctt
      781 ccagaagtac ttacgctctt tcatgagact ggaaagcttt atttccgtct ttttcaaagt
      841 gcaagagaac ggaaagtccc tatattagtg gaccgatttg caccagagca gactcgtccg
      901 acagagcttc aactgctgca aacaatgtgg gaagagacat ctagccaaaa gaaagcggca
      961 gtacacaaaa tagggggaca agagcatctc catttgtggg aagctgaaaa tccaactgaa
     1021 gaagtccgac aattagctgt tgaaatcaga cgactagtca gtgaagaaaa ctaccgttat
     1081 cgtgatatcc aagtgctgac aaaaaatatg tctttatatg gaaatatcct tcggccgatc
     1141 tttcaagaaa tggcaattcc tttttatatc gatgaagaac aaatgatgga aaatcatccg
     1201 ctgatcgaat ggatcaacag tctgttcgct cttgaccgct attcttatcg gctaagtgat
     1261 attatgcgtt ttttcaaaac tgaattattt ctgtcgacgg aagcagaaac cgaacttgat
     1321 gaatggaaac aagagcgaga tgaattcagg aaaaaactgg acatcactga aaatgtagca
     1381 cttgcttaca attatcaagg aacttattgg actcgtgaaa aagactggca gtttgtggcg
     1441 tatgattttg aagcagaaca gattgaagac gtacagtatt tggaagaaca gtcaaatacg
     1501 attagacgat tatttcagaa agctgtacca gtatttttga aaaaaataaa gaaaagcaaa
     1561 acaggacaag aggccgcaaa tgttttttat caatttttga tccaaagtgg cgtacagcaa
     1621 caattattgt tttggcgtaa tcaggaagta gacagaggaa acttagaagc ggcaagaaac
     1681 catgagcaga catggggagc gctgattgat ctgttggatg aatatgtagt aatttatggt
     1741 gaatcggctt ttgactggtc actgttccaa gaaatcatta caagtggtct agaaaatcta
     1801 agttatggta aaatccctac cgcaatcgac caagtgcgaa tcaatcgttt ggaattagtt
     1861 cgaccaaatc aagctgccgt cacttttgca ttagggttga atgatcaagt gtttccagac
     1921 cgaaaagaga caaaaggatt gttgtccagt gaagaacgag aagtattaaa tcaggccttg
     1981 ccagatggac agtttttatt cgacccatca aaggaaagta tctcttttga gcctttccaa
     2041 gcctatcttg tttttctttc cggaacagat cgattatatc ttagttatgc acaaagctat
     2101 gacacagatg gttcgctgaa gatgtctcct tatttgcgta gaatcacaga atatttatct
     2161 gttcccgtcg agagaaaaga gcatttgacg atcgaaagcg accctaattg ttatgtaggg
     2221 acttacagaa gcggaatcaa tacagtcaat aggatttatc gattagcttt agatgaaaaa
     2281 aaacctgtac caaagcactg gcaaaagctc aaagaattag tactgaattc gtcatggcga
     2341 aattttgctt tacagatttt tgaaagtcag gaacatttaa atgttcctgt ttcgatgcca
     2401 gaaaaaatgg cggaagagct ttatggaaaa gagatttata cttcaatttc acgtatggag
     2461 aatttttata attgtgaata caaatacttc atgcaatttg gtttgaaatt gaaagaacga
     2521 actgtttatg gtctaactcc agcagcaact ggtgattttt atcatgaatc attggatcgt
     2581 ttcttcaagc tactgttttc aaaccaatta tccttagtga atatgtccga tgcgcagcgt
     2641 agagacttta ctgaaaaagt gctgcaggaa gtattcggag aacttcggtt tgagatattg
     2701 gatagttctg caagaatgaa ctatatccgc taccagttag gacagacgat tcaaaaagtg
     2761 acatgggctt tgcaaaaaca gagcaaaaat acaggcatgc atcctcttca gacagagatt
     2821 ctatttggtc agattgctgg agcaaaaggt atcacaggtc tggaattacc gttgagtaat
     2881 ggtggaaaac tccatgttcg agggaaaatc gatcgaatcg atgtagcttc tgaacaagaa
     2941 gatacttggc taagcgtcgt tgattacaaa tctagcggac gttcattcga tgtcacagaa
     3001 gcttattatg gcatggctat gcaattattg acgtatctgg acgtagcatt gatggatgcg
     3061 gtacgtttga ctggaaaggc ggctgttaaa ccagcaggat catattattt acatgttcat
     3121 aatcctgtat tgacggagac agatgatgta gagaagaata cattgaaaaa atttagttat
     3181 gatggcgttt ttgtcgatga tcccgctctt ttagaagtac tagatcattc actggaagca
     3241 aaagaacaat cactgatcta tccagtgaaa aaaaatgcga aagatgaata ccaaaaaggt
     3301 agtttcagta aagacaaatt tttcacggaa gaagaactgc aactattcat ggcgcataat
     3361 cgggaaaata tgcggttagc tggagatgcg attacttccg gagaaatcaa actaaatcca
     3421 gcttataaag ataaacagcg gattgcctgc gaattttgtc cattcagaag catctgtacg
     3481 tttgatgtga tgctgaaaga aaataattac caccgtctgg agaaaataga taaggaagaa
     3541 gcactgcgta gaatattgaa aaggagtgaa gaagagtgaa tcaaattcct ttaaaaccgc
     3601 aaaatgaacg attcacagat gatcagtggc aagcgatttt tgatcaagga gataatctat
     3661 tagtctctgc gtctgccgga tcaggtaaaa ctacagtgct tgttcgaaga gtgattgaaa
     3721 aactaaaaat gggttttgat atcgatgaat tattgattgt gacatttact gaagctgccg
     3781 caagagaaat gaaagaaaga attcaagagg cattacaaga atccgtcaat agcgaaagtg
     3841 accctgtacg tagacagcat tttactaagc agttagtttt attgcccaca gcgaatatct
     3901 caaccttaca tgctttttgc ctgacagtta ttcgacgtta ttattactta attgatattg
     3961 atccagtctt tcgcatgtta acagatgaaa cagaaacgat cttgatgaag gaggatgtgt
     4021 gggacgagct gagagaagca ctttatgcag aaaatgatga acgatttttc caattaacaa
     4081 tgaatttttc taatgatcgt tcagatgacg gcttgacgaa tctggtgttt tctctttatg
     4141 agtttgcccg tgccaaccct gacccccaaa aatggctaga acagttaagc gataattatc
     4201 gtttgcctga ggggctagcg aaatctcgat tatatcaaaa acaaatccgt cctttagttt
     4261 tggcagatat ataccaatgt gtacagttat atgaacagat gactcagtta gcacaaggtg
     4321 aaggattaga aaaaatgcat gagcaagtag ctggagaaca acagcagatc aagaatatct
     4381 atgaagcgtt ctcgcaagat cggttagaag aagcttatgc tggtttggaa caactaactt
     4441 tttcaacttt taagagcagt cggaaagcag aattgaaaga aatttccaat gaagtcaagg
     4501 gcatgaggga taaagcaaaa aaactgattc agcagataag taaaagttat tttcctgttt
     4561 ccccaagcca aatggaagaa ttaacggaca aagcactacc gttagtcgaa gaaatgacaa
     4621 aagtcacgca aagcttcatg gacggtttca gtatgcgtaa acgggaaaaa ggtgtgctgg
     4681 atttcaatga tctggaacat ttagcgttgc agattctcac agaaaaaacg aaagatgtat
     4741 ggctgccaag tgaagcttca aaacattatc gaaaaaaatt caaagaagta atggtagatg
     4801 aatatcaaga cgtgaaccaa ttacaagaag ccattttata ctggctaaga gaacccgatg
     4861 atacaaaagg aaatatgttc atggttgggg atgtcaaaca atccatctat tctttccgtt
     4921 tagcagatcc tagtttattt atcgggaaat acgagaattt ctcaaagaaa gaaggcggac
     4981 gtcgaatcgt tttggctgag aacttccgct ctagaaaaga agtcctttct tttacgaatc
     5041 ttatttttga acaactaatg gatccagcag tgggtcaaat caattatgac gaagcagcca
     5101 aactgatcca aggattttca gactttccag agaatgaaca gtttgagccg gaaatcatga
     5161 tctatgaaaa agaacaggaa gaatcagaga ttgagattcc gacggatgat atactggaag
     5221 acaaaacaga aggcgaactt tttatgacag gactaaaaat tcgtcaatta attgattcct
     5281 cctttatgat ttatgataaa aaatcaaaaa aaagtcgtcc gatagagtac aaagacattg
     5341 ttctcttgac acctacaaag aagaacaact taacgatttt agagattttc aaaacattgg
     5401 atattccatt agagatgaat gatgctcaaa actattttca agcgacagaa attcgcacga
     5461 tgatctcgct gctacaatta atcgataatc cctatcaaga tattccgctt gcggctgtat
     5521 tgcgctcacc gatcgtgggt ctgattgaac cagaattagc cagcattcga ttagctaacc
     5581 gtgcacatac ttactatgat gctgtgcttg cttatcaagc atcaaacgaa gatgaattag
     5641 cagctaagct ggaacatttt ggcaagcaac tagagcattg gcgagaactt gctcgccgtt
     5701 cttcgatcac tgacttgtta tgggatatct attatgagac gggatatcta gagtatgtgg
     5761 tcggtctgcc agcaggggca cagagacaag ccaatcttta tgcattggtt gatcgtgcca
     5821 aagcttacga gcaaagttct ttcagaggat tataccagtt cgtgcgtttc attgaaaaaa
     5881 tgcaagaaaa agataaagac ttagcagaac cagtcatttc aatagaagat aatgctgttc
     5941 gcgttatgac gatccatgca agtaaaggct tggaattccc agttgtattt ttgttagata
     6001 tgacaaagga gttcaactta caagacttgc gcaatcggta tgcttttgaa gaaaaacttg
     6061 gagcaggaat tcgctatatg gatcctgaaa cacgagtgtt atatgatacg ttaccgtttc
     6121 aagccatcaa attggcaaag caaaacaaat tgttgtcgga agaaatgcgt aaattgtatg
     6181 taggattgac tcgggcagaa caaaagcttt tcattgtcgg ttcctataaa aataaagagc
     6241 aaatgatcca aacgtggtct gaagcagctg atcatgagga gttagttttt gatcctgcgc
     6301 tacgtttgaa gggcagaagc agcttgatga attggattgg ttacggattg atccgtcatc
     6361 ctgaaatgca gaaatactta gaggaagaaa tttcgacttc tcttcttcaa cattcaaatg
     6421 cacagttttc tatttcatgg atgaatcagc aatcgatcat tgaacaaaga cagttgcttg
     6481 ctgaaaaaga actggttaac ttagatcagc agatgaaaga agatgaaact ttattagcag
     6541 attcgttaca aaaacgtctg gcatatgaat atccttatca agcttcttca caaaccacaa
     6601 gttatcaatc tgtatctgaa atcaaacgat tattcgaaga tccagatgat acacaagaat
     6661 cacgtttgac tctggaaagc agtcagaaca aagcagcatc ccgtcaattt cgttatacgc
     6721 aagaacaact agcagaacca aaatttttgc aaaaagatcg gcaagtgtca gcagctgctg
     6781 ttggaactgc tacgcatgct ttgttacaat tactgccatt agagatgcca actacagaga
     6841 gtatccatca aaaattacag gaattggtaa ggaaacggtt agtagacgaa aaagtcgcga
     6901 aaaaagtaga tgtttcttct attatatggt tctttcaaac agaacttggt caacagctga
     6961 ttgccaacaa agaaaacgta aaacgagaac aacctttttc catgctgctt cctgctgacg
     7021 aagtatttca agactatcca aatcaagaag atgaactatt aatccacgga atcgtcgatg
     7081 gatacctaga agaaaaagat catctcaata tctatgattt taaaacagat tttattttac
     7141 atccggatga tccagcagaa atagatgcaa tcgtccaaaa atatcaagga cagctaagac
     7201 tttaccaaca agcaatgtca gaagctttga ataagccagt agaaaatgtt ttcctaattt
     7261 tgcttcgagt gaaacaaatt ataaacatta acaaataatt ataattgagc aaacttgact
     7321 tatgttttgt aaggattatg ctatactctg ggaagaggtg agtatgatgg agctaacaca
     7381 gacagaattc gttttatgcc ctaaatttga aaaaacattc tctattttag gaaaaaaatg
     7441 gaacggattg attatcgatg tactactcga aaacggtccg caacgctttg ttgagcttgc
     7501 ggcaaaaatc ccgatggtca gtgatcgagt attagtcgaa cgcttgaagg aattagaaaa
     7561 agaaggaata gtcactcgaa actttgcctg tagcgaaaag agccgttcag aatatctctt
     7621 gactgaaaaa ggagcagact tgcaaaaagc gatgcgtcaa atacaagttt ggagcgaaaa
     7681 atggatcaca gaggaagaat gcggttgatt ttgtaaaaaa aatcttgtaa actatagtcc
     7741 atacgaataa gtaaagaacg atgacgaaaa agagtagttt ggctaaacca gttcagggag
     7801 atctgtcgca gactgaaagc agatcctgtg tttgaaaaat gaagttcact ttccgagttc
     7861 gtctgataaa atcagaccgg caattgtcgt taacgaagta agagtgcggt gtaagtcatt
     7921 tatccgcaaa ttaggatggt aacgcgaaga cctcgttcct taacaggaat gaggtctttt
     7981 tgttttgtat ttggaattta aaggaggaac aagcacatgt ctctacaagc acaattagag
     8041 agcttgaaag aagcaacgct cgtcaaaatc acagcggcac aagatttaaa tgaattaaat
     8101 caaatacgtg ttgaaacgtt aggaaaaaaa ggaccgatca cagaagtatt acgtgggatg
     8161 aaagatctaa gtccagaaga gcgaccaaaa gtcggaagtt tcgcaaatga gatccgagat
     8221 cttttgacag aagagatcgt gcgaaaaaaa caagtattag aagaagaagc gttaaatgca
     8281 gctttggcaa aagaaacaat agacgtgaca ctacctggta gaaaagtaaa acatggtaca
     8341 cgtcatatcg tgacacaaat catggaagaa attgaagata tttttgtggg tatgggctat
     8401 cagatcgtag aaggatatga agtagaatct gatcattaca atttcgaacg gatgaatctg
     8461 ccaaaagatc atccagcaag ggacatgcaa gatactttct atatttcaga cgagatattg
     8521 attcggacac atacttcacc agtccaagca cggacaatgg aaaaacatga tttctctaag
     8581 ggtgctctac gaatgatctc gccagggaaa gtcttccgca gagatacaga tgatgcgacc
     8641 cacagtcatc agttccatca aattgaaggg ctagttgttg ataaaaacat cacaatgggc
     8701 gaccttaaag gaacattaga agtcgtaatg aaaaaaatgt ttggggaaga tcgtgaaatt
     8761 cgtttgcgtc caagttattt cccatttacg gaaccatcag tagaagtaga tgtcagctgt
     8821 ttcaaatgtg gtggtgccgg ttgtaacgta tgtaaataca ctggatggat cgaaatttta
     8881 ggagctggca tggtacaccc gaatgtgttg aagatgtcag gaatcgatcc agaagaatat
     8941 tcaggctttg ctttcggcct tggaccagac cgcgtagcta tgttgcggta tggagtgaat
     9001 gatatccgta atttctatca aaatgactta cgtttcttaa gtcagttcaa gggggagtaa
     9061 gaaaatgtta gtttcatata aatggctcaa tcgttatgta gatttatcga atgtaacacc
     9121 aaaagaatta gcggataaga tgtctgtgac aggaatcgaa gtagagggag taacggttcc
     9181 agaagaaggc ttgaaaaaaa tcgttgtcgg tgaagtcaag gaatgcgtac cacatccaga
     9241 ttctgatcac ctttctattt gtcaagtcga tattggcgaa gacgaactgt ctcaaatcgt
     9301 ttgtggagcg cctaatgtga aggttggaat caaagtgatt gttgcacttc ctaactcacg
     9361 tatcgctggt aatgtaaaaa tcaaaaaagg aaaaatgcgt ggccaagtat caaatggcat
     9421 gatttgctca ttacaagaaa ttggctattc agatagtgtt ataccgaaag aatacgctga
     9481 aggtatctat tatatgccac aagaagctgt aaatggcgat ccagtttttc cttatttgga
     9541 tatggatgat tcgattattg aattatctat cacaccgaat cgtgcggatg cattgtcaat
     9601 gcgtggcgta gcttatgaag ttggggcaat ttatcgtcag actcctacat tcgatgacga
     9661 aaagctggta gaatcctcac gtcctgctag cgataaaatt caagtggaag ttgaagataa
     9721 agaagcagtt cctgcttatc aaatcagaat cattgaagga gtaagaatcc agccaagtcc
     9781 tcaatggctg caaaatcttt tgatgaacga aggcattcgt ccaatcaaca atgtagtaga
     9841 tgtgacgaac tatattttgc ttttattcgg acaaccacta catgcatttg actacgacaa
     9901 attgaaatca aaagagatct ttgtccgccg tgcaaaagaa aatgaaacat tagtaacact
     9961 agatggagaa tctcgggaat tgacaactga caatctcgtc attactaacg gcaaagaacc
    10021 agttgcttta gctggtgtca tgggagggtt agactcagag atcacgaatg aaacaacaac
    10081 agttgcacta gaatctgctt tatttgatcc aatttcgatt cgtcgtacag caaaacaatt
    10141 taatttgcgt agtgaatcct ctgcacgttt tgaaaaagga atcaaccatg cgactgttgg
    10201 agaagccagt gaggttgctg cagcaatgat cgtttctctt gcgggtgggg aagttctaca
    10261 aggcgcggtc aaaggctcag agatcaaagc agaagacgta tcagtgaaga tttcacttga
    10321 acgcatcaat caatatttag gaacatctct tgtcgttaaa gaagtaaatg agatttttga
    10381 agcacttgga tttagctatg aagaaaagga gtatgtttat tccgtagtag taccaccaag
    10441 acgctgggat atccaaatcg aagcagatat tatcgaagaa gttgctcgta tttatggata
    10501 cgaccagttg ccatctacac ttccaagtgg agaaacggtt gctggaagct tgtcaaaaga
    10561 acaagaaact actcgtaaaa ttcgtacaat ctttgaagga agcggattga gcgaagcaat
    10621 cagttatgca ttgacaacag aagaaaaatc aagacagttt gttataaatg aaagtaatat
    10681 tacacgtttg gactggccaa tgagcgaaga acgttctgta ttacgaatga atttgatcag
    10741 cggactatta gatgatatca gttataacac tgcccgaaaa aatacagaga ttgctttata
    10801 tgaaattggt cgggtgttct tccaagaaaa tgatccacgt acacatctgc cgcaagaagt
    10861 aaaacatgca gcaatcgcta tttctggtat ttgggaagaa aaagattggc agacgaaagc
    10921 tcaaaatgta gactttttca ccatcaaggg tcttttagaa aacttatttg aacaactagg
    10981 catcactgaa gatgtccatt atcaaacagt gacagaaatg aaggaaatgc atcctggaag
    11041 aactgctgcg atttaccttg gcgaaaaatt tattggtttt gtcggtcaag tgcatccaac
    11101 gattacaaaa agttatgata ttccagaaac atatgttgct gagtttgatt tatctgctat
    11161 agtggaagct gctcaaaaag ggatcgtttt cgaagcagtg acaaaattcc cagcagtcag
    11221 ccgggacatc gctttgcttg taaaagaaac tgtgacaaat caggaattaa cagaagttat
    11281 ccgttctgct gccggaagat ttttaacaga tatccaatta tttgatgtct atcaaggaga
    11341 aaacattgaa aaaggacata aatccatggc gtatagcctg acattcgtca acccagaagc
    11401 aacattgaca gatgaagaaa tcaataaagc aatggaaaaa gtcgaaaaag cgttggtaga
    11461 aaacctagag gcaagtattc gctaagaagg aaaatatagt aagtaacgat caagtagctt
    11521 ttgaagacca tacatttgaa aaaaagatgt gacacaactt cgtcacatct ctttttttga
    11581 ttttaaaaaa ggaatagcac agagacgaga taaaaaaaca ggcctttaca gcctgctttt
    11641 ttagagtaaa tgaattaatg gaagtaattg attcccattg ctgattttac ttcagaaagt
    11701 gtttgtgcag ctactttagc agcttcttca ctacctttac gtaagatttc cataacagct
    11761 gaaggatctt tttccaattc ttctcggcgc gcacggatcg gtgcaaattc agcttccaat
    11821 acatcaatga ggtatcgttt gatctttaca tcaccaagtc cgccgcggcg gtaatgagct
    11881 ttcatttctt caattgcttc tttatctgtt ccgaatacat ccaagtaggt gaagaccata
    11941 ttcccttcta cttgtccagg atcttgtaca tggatgtggt ttggatcagt atacatactc
    12001 atgacttttt tctgtaagac atcggcagga tcggagatat aaataccgtt tcctaacgat
    12061 ttactcattt tgccatttcc atcgattcca ggtaatctac ccattccttt tggtgggaat
    12121 acgccttttg gttccaccaa gacttcacca tatgtatgat tgaagctttg tacgatttcc
    12181 tgtgtctgct ctagcattgg tttttgatcc tcaccaacag gtacaagatt cgctttgaat
    12241 gcagtgatat ctgctgcttg agaaacagga tagataaaga atcctgtagg cacgctttca
    12301 ccgaattttt tttgttcgat ttctgttttg acagtcggat tgcgccgaac gcgtcctacg
    12361 ctgactaagt tcaaataata catggttaat tcagctaatt ccggaatttg tgattggata
    12421 aataaagtag attttgcagg atctaatccc acggctaaat agtctaaagc aacttgcaag
    12481 acatttgatg aaaccttctc aggattttta gcattatctg tcagtgcttg catgtcagca
    12541 atcatgacaa acagttggtt gtcttcatct gcctgcatag ccactcgggt ttttagagaa
    12601 ccgacgtaat gccctaaatg cagcttccca gttggtcgat ctcccgttaa aataatattt
    12661 ttcaaaaaag acacctcttt acgatttgta agtctgagca cttaatatct taccgttttt
    12721 cctgaaaaga aacaacagtt cctaaggaaa aaacaaggaa agaggataaa at
//
LOCUS       DAJSKS010000076        12742 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJSKS010000076 DAJSKS010000000
VERSION     DAJSKS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.078"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ON396_001787"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ON396_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR3113869.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ON396_001788"
     CDS             complement(322..891)
                     /locus_tag="ON396_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCR3113870.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ON396_001789"
     CDS             1077..1979
                     /locus_tag="ON396_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3113871.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ON396_001790"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ON396_001790"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR3113872.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ON396_001791"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ON396_001791"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR3113873.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8322)
                     /gene="dnaE"
                     /locus_tag="ON396_001792"
     CDS             complement(5002..8322)
                     /gene="dnaE"
                     /locus_tag="ON396_001792"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR3113874.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8553..8738
                     /locus_tag="ON396_001793"
     CDS             8553..8738
                     /locus_tag="ON396_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCR3113875.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8800..9996)
                     /locus_tag="ON396_001794"
     CDS             complement(8800..9996)
                     /locus_tag="ON396_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR3113876.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10166..11602)
                     /locus_tag="ON396_001795"
     CDS             complement(10166..11602)
                     /locus_tag="ON396_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCR3113877.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11895..12704
                     /locus_tag="ON396_001796"
     CDS             11895..12704
                     /locus_tag="ON396_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3113878.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4013 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tactcttgct gtcatccacc caataagcat ctgacagaac
     5101 tacttttcgt tgcgtttttt cgtgatacat aactactggt atatatcctg ggtgtttctg
     5161 aaaaatcact tgtaattgtt tcattatctc gggatcttcc cgttcttctg taatacgcaa
     5221 atagcaagtt tgtgaaggat actgggcttc taactggtta ggatcagcaa agctttctcc
     5281 aattagttgc agttcctgat tatatttgct gatttcgctc cgcccttgaa caaagatcac
     5341 tcgatcaacg gcaaacaaat tacgatattt acgataaaga gttggaaaaa ctgtgacaga
     5401 aatttctcca ctactgtcgg ttccttcaac aaaggccatc agttctcctt tttttgtccg
     5461 aatttctcta acttccttga tcatcactaa taaattgact ggttgattag ggacgatatc
     5521 tgtaatcagc tgaacttgtt tagctaaacg aagacgatca aacccttctg caggatgacc
     5581 tgacaaataa atacctaagt actcctcttc cagcttcaag cgttcttcta gtgtatagtc
     5641 agcgacttct tcttctttta atgccatgat tcccagcaag tcaagacttc caccactgta
     5701 aacgatattt tggatttttc cttcgagatc aagcatcaat tgtttcctgt taggagataa
     5761 ttcatcaaaa actcctactg ctgtcagtga ttgcagtaac tctaatttca gccaacgctt
     5821 atcgatccgt atgaggaatt gatccactga ctggaacggg ccattttgtt ttctttcgtt
     5881 taatatatct tctacaaaat cacgccgaat accttttatc gctgtcagtc cgaatctgat
     5941 ttcttcctta ttgaccaact caaaactgga attactttga ttaatggacg gcgaggaaag
     6001 tttgagttta tttttacgtg cttctgcaat atattctttc aattttgatg tgttatgccg
     6061 aacagaattc atcaggctgg taaagaaagc acctggatga tgggctttta gatacgccat
     6121 ttggaatccc acgaatgaat aagcaaatgc atgtgaccga ttgaatccat agtttgcaaa
     6181 tttttcgata tattcatata cggtcgctgc tgttttttcc gaatatccct gttgtaatgc
     6241 tcctgataca aaatgcgact tttcttcatc taatacgtct tttttctttt tactcactgc
     6301 ccgtcgcaga atatctgact gccctaatgt aaatccagcc atagtcgagg cgatctgcat
     6361 gatttgttcc tgatagacga tcactccata tgtgtttttc aaaatcggga tcagtgaatc
     6421 atctgggtag cgaatggctt cttttccttt cttcctggca atgaaggtat ctatattttg
     6481 catagggcct ggtcgataca atgcattgac tgcggcaata tcttcgatgt tttctggatc
     6541 taatcttcgt aaaacgttcc ggataccagc agattcaaat tgaaatactc ctgaggtttc
     6601 gcctcgttga aacaagagca gcgtttttgc atcatttagc gggatctctt ttaatcggat
     6661 ctcataccct tctactcgct taacagcttt caaagtaaaa tcaataatcg ataagttgcg
     6721 tagtcctaaa aagtccattt tcaaaagacc gattgcttct acctcattca tcgtatactg
     6781 ggtcagaaaa gcttcatcag aaccgcgttg gagcggaact agttcaagta gattcttatc
     6841 gctgataact acacctgctg cgtgagtcga aacatgtctt ggcaatcctt ctaaaatcac
     6901 tgcagttttg aataaaagtt ggtttttttc gcttgcctga actaattcta ctaattgatg
     6961 cgaagtttta taagcttggg tcaatgtgat tttcaattga ttgggtattg cttttgacca
     7021 gcggtttgct tcactttgcg ataaaccaaa cactctagct gcatctctaa gaaccatttt
     7081 tgctgccatt gtgccaaatg ttgcgatttg tgccatatga tattgtccat acttttctcg
     7141 aacataattc aatacttctt cacgtcgatt gtcaggaata tccaaatcga tatctggcat
     7201 cgtaaagcgt tcggggttta aaaagcgttc gaacagcaaa tcatattcta atggatccac
     7261 atctgtgatt tctaacacat aggctactaa tgaacccgca gcagaaccac gacctgcacc
     7321 ggtaacgatt ttgtttctat gagcaaaagc cattacatcc catacgatca agaaataatc
     7381 atcgaatccc atttgatgga tgacagataa ctcgtagtcc aaacgttctt ggtattcctt
     7441 tgtatagtca gtgattcggc gttccacttg cgaatagcat aactctctta aatattctcc
     7501 agcttgtttt ccattaggaa ctggataatg cggaagtaat ttctgatgga actggaattc
     7561 aaatgaaatc gattcagcta aacgctgggc gcctattaca gcttgggaaa gacccgcttt
     7621 ttcgaatcgt ttgatcattt cttgttctga aagaagatag acatttgctg ttagtggtaa
     7681 agatgcagat aaattttcta aaggtatttg gcttccctct ttgatatgtt tcatcacttc
     7741 aagtgcaaaa agttcttctt gttgtaaact agcgatggga tgtaatgcac aaagcgaata
     7801 attttctttt atcacatact gataccattc aggcaattga tcaggcgtgt tttctgtcaa
     7861 gccgatataa aaatttctag taaaaacatt ttcaagtaaa aactgccaat gaccgaaaaa
     7921 gttatcctct ttattgaaag cctcagaaac agaatgattt gccggaacaa caaacgctag
     7981 atggtcggtt tctgtcattt ctgcaagttc caatttttct tggatcattt ttcggctgga
     8041 aagtttcatc agctgctgat aacctatttc atcttttgcg tacgcaagca agccataatt
     8101 tttttcttca taagtataac taagatgtaa tccaatgatt ggctgtactc cttcttctac
     8161 acaaatacga tagaattcag gtgcaccgta taagacatct ttatccgtta tggctaatga
     8221 agtgtagcct tgtttttttg cttcttgtac atattctcga atacgtatcg tactttgtaa
     8281 aagagaataa gaagtaattg tataaagttg tgggaaaaac atcttgcatt ccgccttttt
     8341 tttcgttaaa attcactagc gttaaaaggt ttgatccttt tactttcatt aagatagcag
     8401 gcgctgaagt tgattattcc acttcatttg cttgcttgta atctgtagga atgagttctt
     8461 tcgcaagaac tcaaatttta ttgagatttg gtctcgtttc tttacattca aagaaaatct
     8521 ggtaaaatta agatagaaaa gaggtgttct ttatgcgata tatcgttaca ttattctggg
     8581 ctgtcgttct aggtcaagta gtcggttata ttggtgctgc attaacaagc ggaacatatg
     8641 attttacttt gacaacaatc atttcattca ttgcgggtgt cattatcctt ttgattggtg
     8701 cagttgcgcc aagaaaagaa acctctgccc attcgtaaaa aaacagccaa gagcttgaga
     8761 tttctcaagc tcttttttat tttctactcc tcaccaccat tataatctgc tgcgaagaac
     8821 tttacgaaag ttgaataaag aaagaagaat atttgacaga acaaacaaac ttgtcacaat
     8881 aaaaacagca ctgtaattat aagcatgcgc gactgtcgat cctagcatgg gacctaaaac
     8941 ttgtccaaag ttgctaaaca tctgattgta gctatagatt cggctgactc cttcttgagg
     9001 cgtaatctta ctgatcagtg tattgacaga aggcatcaaa gcgccagttg aaaagcctaa
     9061 aataaagcga agtataccta attggagcgg tgtagtgacg aatgccatag gtaaataaca
     9121 gcaaaatgat aaaatgagtc ctccaattag aactttttga ttcccaatct tatcccctag
     9181 ttttcctagt ttcggtgaag aaaagacagc ggaaacacca gctatcgaaa caatcaagcc
     9241 gctgacaaaa aggatatttt ctgtacttcc gctcaattca cgaatatata aggtcaaaat
     9301 agggctgatc gtagtgattc ctatctgtaa aatcaaggtc gtaatgaaga gaccgatcaa
     9361 aatagagaga tgatccattt ttgaaaaaat ttctttggtg cttatcagat cttttttctc
     9421 gattggatgg aaatcttctt tgactaagaa aatggtcaat accgttgtga tcatcaacaa
     9481 agcccctgtg atcaagaata cattttccat accaaaccac tgagccagtg caccgcccat
     9541 tgaaggtccg atcagatttc cagcaatcgc tccggtagac aatgttccta acgcccatcc
     9601 gctcttttct ctaggtgcct gtgaagcaat catcgctgtg gcatttggaa tatatccaga
     9661 caaaatccca ttccaaaacc gcatgatcaa aagccaatac gcgtttggta caaaagccaa
     9721 agatcccatt gttaatgtca tccctgctgc tgcacggatc atcatgagtt ttcggccttt
     9781 tcgatcagct agatttcccc agattggtgc aaccacggca gatgcaaaag cagtcacaga
     9841 gattgctagt cccgaaaaca attcgatttg atcttttggt gttcctagtt gctcaacata
     9901 aactggtatg aatggcataa ctaaactaat acttgcacca gtgaaaaaac agccaatcca
     9961 cgcaatcact aaattttttc tccaattaat ctccatctat catccctctt tcgtctcttt
    10021 tatcatacct attttcgaat aaacaagcaa cgatctcgcc agtaaaaaga gaaaaatccc
    10081 gcaaaggtca ttttatatgt aacgttcttt ttatgtaaaa caaaaagaga tcagaacaaa
    10141 tactgttcca atcccttctc tttagttaaa ttgttgctag aatgatgaag ttgataatga
    10201 ataaaatatc aacgatccac aaagcaacag aaacttgatt gtattttcct ttgattactt
    10261 tcacaattgt atagaaaata aatcctgctg caatcccata ggaaatgcta tagcacaatc
    10321 ccataaagat cgatgcaaag aaggccggta aagcttcttc catatctagc catttgatgt
    10381 ctgcaaaaga agccatcatc attactccaa ctaaaatcaa tgctggcgca gttgcttgat
    10441 ttggtacaat ggcgatcagt ggagagaata aactacttaa tgcgaatagc actgccacta
    10501 ctactgaagt caaaccagta cgtccaccgg ccccgatccc agctgcagat tcgacataag
    10561 ttgttgtatt cgatgtacca aagatagccc ccacagaagt agcgatcgca tcggcaaata
    10621 gtgctttatc catttttgtt ttgaacccac gaccatcttc caacgcgatc tcgtcttctt
    10681 ttgagaaaat acctgtgcgg cgtcctgtac cgataaatgt tccgatcgta tcgaaagtat
    10741 cagataaact gaatgcgatg atcgtcatca atacttgagg aatcttggaa gaatcactga
    10801 ataaagattg catgccttca gaaccaaatg ctgcaccgaa agttgttcct aattctttaa
    10861 atgaattacc taacgaattc gtttgccaat caatggaact taaatcaaca acacccataa
    10921 aaataccaag gactgtggtc gctacgatac cgataagaat agccccacgg acatttttta
    10981 caactaaaat ggctgtcagt accaaaccaa tcacagccaa taagatagga gcgttgtcaa
    11041 agttggctaa cgcagggacg attccaccat ttatcgtaac atttgtcgct tttccgcctt
    11101 caacgacaga gctcgaaata gctgactggt ctgctgaaaa agataagaat cctgcatttt
    11161 tgattccgac atacgcaacg aaaataccga tcccgccacc aatcgcatgt tgaagacttt
    11221 cgggaatcgc ccgaataatc atttttcgaa tatttgtgac agtaataaaa atattgatta
    11281 atccgcaaat aaacaccatt gccaaggctt gttgccaaga ataaccaagc ccaaaaacga
    11341 ccgtaaaagt gaaaaatgcg ttcaatccca taccaggtgc ctgagcatat ggtacatttg
    11401 cgaacaatcc cattaccagc gtaccaataa tcgatgcaat gattgtcgct agaaatactg
    11461 cttggaaggg catacctgat gctgacaaaa tcgtcggatt tacgaataag atataactca
    11521 tggcaaagaa tgtcgttact cccgccatta tttcagttga tacatttgta ttgtttttct
    11581 ttaaaccgaa aaacttttcc ataaaaagtt ccactccctg aatttttttc tgatggaaaa
    11641 ctataataat aaaaaataag taaaataggg caaatccatc gaacaatttc aaattatata
    11701 gacgtttcat tcgcttttca acgttttttt aacaaaaaaa gcgaaaataa tacttaacgt
    11761 tcgtgtttag cttatgttta gctatttata tagccataac tgtagttttt gttaggaata
    11821 gctgattgct cttgttaatt acctaaaata tgctagacta aaacaaatgc tagttgagga
    11881 aggaaggacc cttattgaat aaaaaattaa ttgccatcga tctggacgga actaccctca
    11941 atcaagattc gatgattaca tctaaaacaa aagagactct aaaaaaagca atcaatgccg
    12001 gtcatcatgt cagtattgct acaggacgcc cattccgaat gagccatcag ttttaccaac
    12061 aattagaatt aacaacaccg atggttaatt ttaacggcgc attagtacac atccctaatc
    12121 agcactggga tggtgaaaga gaaacgttga tcaatcgtga aatcgctttt gaaatcttat
    12181 cgcaaaaaaa gcagctaaat ttagatttta tcgctgctga aaatcgtgat acttttttta
    12241 ttgacagttt tgatttcttt gatgaaaaaa tttttgcttc ttctcgtcca ggagagaaga
    12301 atttgctttc tcctaaaaat ttgacgacaa atcctacatc tttgcttgtc cgaacagaca
    12361 aacgttttgc aggagctgta tctgctgaat taacacgtca atttggcagt tatgtcgatg
    12421 ttcggacatg gggtggacct actgccatct tagagattgt ttcaaaagga atccaaaaag
    12481 caaaaggcgt gcaagagatt gctaactacc tgtctatcga tcaaaaagac gtcattgcct
    12541 ttggtgacga gcataatgat ttggaattgt tggattatgc aggttgggga gtagctatgg
    12601 ctaacggtac tgaccaactc aaaggcattg caaatgatgt cactcctttg agcaatcagg
    12661 aagacggttt agctgtttat ctggaaaaac tacttaaact ataatagaga attagattcc
    12721 acaaaaaaga ggttgtgaca at
//
LOCUS       DAJSKS010000077        12695 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJSKS010000077 DAJSKS010000000
VERSION     DAJSKS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.079"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..547
                     /locus_tag="ON396_001797"
     CDS             <1..547
                     /locus_tag="ON396_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3113879.1"
                     /translation="NVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRMSDI
                     IGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQGRH
                     ITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQAITK
                     RYIGITEDEIGASLRGFKLGV"
     gene            complement(720..1193)
                     /locus_tag="ON396_001798"
     CDS             complement(720..1193)
                     /locus_tag="ON396_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3113880.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1205..1741
                     /locus_tag="ON396_001799"
     CDS             1205..1741
                     /locus_tag="ON396_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3113881.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1781..2152
                     /locus_tag="ON396_001800"
     CDS             1781..2152
                     /locus_tag="ON396_001800"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3113882.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2436..3707
                     /locus_tag="ON396_001801"
     CDS             2436..3707
                     /locus_tag="ON396_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR3113883.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            3952..4977
                     /locus_tag="ON396_001802"
     CDS             3952..4977
                     /locus_tag="ON396_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3113884.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5271..6380
                     /locus_tag="ON396_001803"
     CDS             5271..6380
                     /locus_tag="ON396_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR3113885.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6463..7359
                     /locus_tag="ON396_001804"
                     /pseudo
     CDS             6463..7359
                     /locus_tag="ON396_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7375..8199
                     /locus_tag="ON396_001805"
     CDS             7375..8199
                     /locus_tag="ON396_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3113886.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8231..9541
                     /locus_tag="ON396_001806"
     CDS             8231..9541
                     /locus_tag="ON396_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HCR3113887.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9689..10948
                     /locus_tag="ON396_001807"
     CDS             9689..10948
                     /locus_tag="ON396_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3113888.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            10958..11830
                     /locus_tag="ON396_001808"
     CDS             10958..11830
                     /locus_tag="ON396_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3113889.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11842..12672
                     /locus_tag="ON396_001809"
     CDS             11842..12672
                     /locus_tag="ON396_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3113890.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
BASE COUNT         4270 a         1894 c         2467 g         4064 t
ORIGIN      
        1 taatgtacaa ccgttaagga caaatgaaga gatacaagat tttcagtttt ggctaagaag
       61 aacaagtaat ccggaaagag atagtttttt atttttgttt ggaatcaata atgggttacg
      121 tatgtcagat attataggat taaaagttgg agatatacga ggcaaaagta agcctattat
      181 tgttgaacat aaaacaggta agcgaaaacc gatttttata gataatttga gagaagaaat
      241 tttattatat actgaaggta aagaagaaaa tgattggtta tttccgagtc ggcaacaagg
      301 tcgacatata acaagggatc gtgtctatca gatatatgca gatatagcag aaaaattagg
      361 acgagatgat ataggaacac atacattaag aaaaactttt gggtatcact actacaagaa
      421 aactagagac attgctacct taatgtttat ttttaatcat agctcgcaag caattacaaa
      481 aaggtacatt ggtataactg aagatgaaat aggagcatca cttcgtggat ttaaactagg
      541 tgtataattt aaaaattttt ataaacagta aagacaaaga ttaaaagcta tttttatcta
      601 gaacgatttt atttcataaa gttcgcggaa taaaagctgg ggttactggt acttggtata
      661 ggttcgtgtt atataaacta agtaattaaa taacattttt aggttggaaa aggatgaaat
      721 taaaaaaaga gaatcaatac ggtctcaaaa acgcaaacgc tgcggaataa gaaaaatact
      781 gataactcaa cgtttagcag actatctaat agggatagtc tgcttttttc gtttttgacc
      841 atacttttga ccttctgcaa acttccttgc ttttcctctg tagaactcat gaaatctgca
      901 aaacgaattg ccgtttcctc tgtctgcttt ttcgcgatat gggtgtatat atcatccata
      961 gtggttttaa tatctgtatg tccaagtcga acatacactt ctttgacaga cgcaccagat
     1021 tcaaagagca gactacaatg ggtatgacga aaaccatgaa gtgtgatatg ttttaagtta
     1081 ttctctttaa tgatgatttt caaattgtga tttacatagt ctaaataaag gggcttattt
     1141 tcttctgatg aaaacaaaag ttgttcacca tcttttatag gtttgattct catttttaga
     1201 tattatgccc gcacgtattc atcacatttc tattgtgaat cgctttattc gaccgacatt
     1261 tgacttttat cataatatac tcggtttaaa attactaatg aaaacaatta atcaagacga
     1321 tcataccatg tatcacctgt ttttttcaga taatcaccac cgtgtgggaa ctgaactgac
     1381 tttttttgaa gtacaagaag ggaacaacca gttttttgga accaatacga ttgaacgaac
     1441 gatactaaaa gttccttccg aagcatcatt gcatttttgg gaaatatatt ttgaaacaca
     1501 aggcgtttgc cattatggaa tcgaatcatt ttctggacga cctatcttac gttttgaagg
     1561 gccagatgca acacgattag ctttagttcc cttacgcgaa tttgaagata ttgatgatta
     1621 tttcccgtat gtcacagacc agatccctac agagcatgcc attttaggaa ttgatgccat
     1681 tcaactacgc gtacaatatt ccgatgcgac tgaacgagag ttattgtcgg taggctggta
     1741 gcacaaagaa tacgtcagtt tcttttcggg tcatcaacga gtgacaatct tagaaaatca
     1801 agacactcag ttctatcaag aagttcagat tattcaagat cgtgagaatc cagttgccat
     1861 tgaagggatt ggtagcgtcc atcatgttgc gtttggcgta gaaaataaat ctgatttaca
     1921 acgcatcgac aaacaattac aagagcgcaa ttttataaat tcaggaataa aagatcgtga
     1981 attttttgta tcactttatt atcgtgaccc aaatcaatta ttgattgaaa ttgctacagg
     2041 ggaaggaaac ctagatgcaa aagcgtatga aaatcaaagt ccacgctttg aagaaattcc
     2101 tttattttta ccgcagtact taaatttcat tcgtgaacaa gtggagcaat aattagctaa
     2161 tcaaatccat gacgaagagt aagaggttga ctcataagtc aaaaaataaa gaaaaacgca
     2221 tcaaatccat tctaaagaat gttgatttga tgcggttcct atttttataa actatcattt
     2281 tcgactaata aaactactta tgagtcagcc tctctttttt tcttgatttt tatacctctt
     2341 tctaggtaaa ctaaacatct gccaaaaata ccgtgtctca ttgaaacatt ctcaactatt
     2401 tcaaaataaa ggggagtacc aaatgaacga gtatattgaa attatccacg caagagaaac
     2461 aacttaaaaa tgtcagtctc aaaattccca aaaataaaat tagtattttt accagcgtat
     2521 ctggttcagg aaaatcatcg attgtttttg atactattac ccaagaagct ggtcgtcaac
     2581 tgaatgacac ctatgatagt tttacccgtt tatttttacc taaatacagt cgtccagagg
     2641 ttgatgagat tgataatctt tctacggcga ttgtggtcaa tcaaaagaag attggtggca
     2701 gtgcgcgctc aacgttaggg acgattacta acatcaatgc gttatttcgt gtgttgtttt
     2761 catgttttgc aacacctagt ttaggatatg caaatgctta ttcatttaat gatcctagtg
     2821 gaatgtgttc tgtctgtcag ggaatcggta agacgattgc atttttaata ggattgcttt
     2881 atttttcggc tattgataat gctgattttg tattttctga tccatttacc gcagaagttt
     2941 tgtctggaat tgaggaacag gcaaagaatc atggttattt tgtattggtg cataatgtta
     3001 attcagcaga agatgttaaa actattcaga gaaattggcg ttttgagggg ttcattgttg
     3061 ttggtgtacg tgtaaaagaa tttttggaat taaataacgt ttttgatggt cctgttgttt
     3121 atattgatac gcatctttca aaagagacat taagagaact tgaacaacaa atgaatcgtt
     3181 tatttgtaaa tacaaatgat taccaagcta gtacattggc agtggaacat ttgattcatt
     3241 atggtcatga gaaaatagcg tttctttctt ttgactacat tgtaggtgaa ccaagtgtta
     3301 ttcaagaacg acatcaagct tatatcgata cattaaagaa atatcattta acctttaaat
     3361 cgcattggat atataacaac tctgaatttg agcgaattta tcaagaatta gctgaattta
     3421 ctgcaattgt tgttactgga gattatttag cggctaaatt agtaaaattt ttaaaaaata
     3481 agaataaacg agtgatggaa caattatcga ttattggatt tgatgatatt acttttgcag
     3541 aattaatgga tcctgcgttg acaacaatac gactaaatcc aggtaacaag ggggcgattg
     3601 cttttcacca actgattgga ctggccaaac aagaagaaat aaaaaatcaa gtactactat
     3661 tagagggaga attgatttct cgagactctg ttcacaaaat caaataaaaa agctctgtga
     3721 aataagtata gttaacaata aaatatccga acgatagaga gaatgaactg tgatctagca
     3781 gttcctcgtc gcaaattcac tagaattagc attatttact ctaaattgtt cggatatttt
     3841 ttcttgtttt taattatgat tacagtgcct atttttaacg aaatttgctt attttcaaaa
     3901 tgtatgatta tatgttagta aacactttca aatatgaaaa gaggagctat tatggtagga
     3961 attagggatg ttgcaaaacg ggcgggtgtg tccattgcaa cagtatcgcg cgtgctaaat
     4021 gaggatccga cactttcagt aaccgatgaa accaaacgaa agattgcaga atcggttaat
     4081 ttttatcatt atgtcaaaaa aagtcccgtt gcaaaaaatt taacaaatat tgcattaata
     4141 acgacagttt ctgaagtaga tgagttggaa gatccctatt ttcgaacgat tcgcagaggg
     4201 attcaagtcg aagcagaaca aagcaaagtc aatttgaaga aaattattcg tttgtcagaa
     4261 acaccgttgg agcaagacga tttgctaaaa tgtcaaggtg tgttgattat tgggcaggtg
     4321 ctatcttctg tgattgaaga agtatcagca attaattcga atgtcgtagt gattgatgac
     4381 cctagtattg aagctgaagt agatgcagtt tatactgatt taacaaaagc aacgaatcag
     4441 catttagatc gattgtataa aaaaggacac cgaaacattg cgtttatcgg aggaggacgt
     4501 gttcgtttag atagccaagg aaataaacac gtgtcagatg atgattatcg aaaaatagcc
     4561 tatgagacgt ggatgaaaca aaaaggatta gaaaaatata gtcaaattta tttagaaggc
     4621 tggaccacac ttgacggaat gcaggcagca gatttacttt tgaaaaaaca tgctaaagat
     4681 aagttgccga ctgctatcat ggtaggaaat gatcctattg ctgtgggggt ttatcgttca
     4741 cttcaaaaaa atggtctaca tattccagaa gatatttcga ttgtgagttt tgataatatt
     4801 gaagttgctg aatttttaac acctgcatta aatacggtga atgtgaatac agaagaaatt
     4861 ggaaaactag cagttcgcat ggttaatgaa cgaataaaag aagttagaaa tgtttcgatc
     4921 aaagtgattg tttctaataa tattattgtt agagaaagtg aaaaaaatat cgattaaaaa
     4981 agtaaacatc caaaaaaggg tgtttacttt tttgtttact aatattttta gtaaaaaatc
     5041 tcgacaaaac attaagtaaa cagttatggt gttgttaaca aaaagaaaac gcattcaaaa
     5101 caggaggaat taagatgaaa catttcaaaa aaatagcttt aacagggata gctgttttaa
     5161 ctgggacatt actcgcagca tgtggtgggg gagatagtaa ttctaaagac gggcaagtaa
     5221 ctttaagcta tgcgagttga gatactgacc aagctccagg actaagaaaa atgttagatg
     5281 aatttgaaaa acaaaatcct gatatcaaag tagaaatgga gacaacgcct tgggatcaat
     5341 attgggttaa attagaagct gcaacaacgg gaggtaatat gccagatgta gtaaccatgc
     5401 actctagcga atcctataaa tatatgtcac agaatgcttt gatgggactt gatgatgtta
     5461 tcaaagaaaa tgatattgac atgggaaact ttactgaagg aattgctgat ttctatacgc
     5521 ttgaagacga actatacgca atccctaaag atgcttctgt agtggggcta tggtacaaca
     5581 aagaattatt tgataatgca ggtgtcgatt atccagatga aacatggaca tgggaaacac
     5641 tcagacaagc tgcaattgat ttaacagatg aatcaaaagg catctatggt tttgcatcac
     5701 atattggaac agagactggc tactggccat ttatttatca aaatggggga gaagtatttg
     5761 ccgatgacaa tacaaaatca ggtttgaata ccccagaagc acaagaagct ttagcttttt
     5821 atcgagatat tgcacttgtt gatcatgctt caccaacaat tacagaatta caagatcctg
     5881 ataagttaag tcgttttcaa tcaggtagat tagcaatgat gattgaagga aactggtttg
     5941 caccgggatt tttgaaaaat gactatctag ttgaaaatgt tgcttctgcc gtgttaccta
     6001 agggaaaagt taatgcaaca attacaaatg gacttggttg ggctgccagt gcgagtacaa
     6061 aatacccaga agaagtgaaa aaactaatgg catttttagc tagtgaggac gccaataaaa
     6121 tccaagcgga aacgggggca tcaatcccag cactaaaagg ctttgaagac gagtgggctg
     6181 cttcttcaga tacaattgat ttgagtgctt ttggaaaaat gttggagtat ggagtccctc
     6241 gtccatacaa taaagatgga ttacgagctg aacaagcaga agttcaaatt attaaccgta
     6301 ttctgtctgg tgaagtaact gttgaagatg gaaccgctca aattgaaaaa gaagtcaatg
     6361 cgatattgag cggaaattaa attagttaat tatacaatat acaggcgaaa ggattctatt
     6421 tcgtctgtat attaatggat gaataccaaa aagggggata ttatgaaaaa tcgaattttt
     6481 ggaaaatatc gtaaagatca agtgatatgg ggctatattt ttatttttcc aacggcatta
     6541 ggattaattt tattaaatat atggccagca attcaaacag caattttaag ttttcaaaaa
     6601 acggtagggt tcggtgatac tgaatgggtc ggattaagaa attaccaaaa aatgtttgca
     6661 gacggtgaag tttttcagtc tttattaaac acattaattt atgcagtggt ttcgatacct
     6721 agtattgttg tattatcctt gttggtggca gttttaatga atagaaaagt taaaggattg
     6781 tcgatttatc ggacaattta ttttctacca gtcgttgctg caccatccgc agtcgcaatg
     6841 atttggcgct ggatgttcaa taatgattat ggactgatta ataacatgtt ggctaaaata
     6901 ggtctcgatg gaccagcatg gttaacggat ccatcgattg cgatttattc tattatcatt
     6961 gtaggagttt ggagtgctat tggttataac atggtgttat tactagccgg cttataagaa
     7021 attcctaaag attattatga agcagcggaa attgatggtg ctagtccaat ccagcagttt
     7081 ttcaacatta cgttaccatt agttacacca acattatttt ttgtagttgt aacaacagtt
     7141 atcaatgctt tccaagtatt tgatgttatt tttatgatga ttactcctaa tagtaccgcc
     7201 atgtttaaat cacaatcatt agcctattta ttctataaac attcatttat tttaaatgac
     7261 aagggatatg gttcggcaat cgttatgttc cttttagtga ttatattaat tattactgca
     7321 attcaaatga agttgcagaa aaagtgggta aactattaag gaggctatca aattatgaaa
     7381 aataaatttt tttcaaatgc gccaatccat atcatgctta ttttaggtgg tattgcaatg
     7441 gcattacctt ttatttggat gattttaact tcagggaaaa cgttggttga atctacccaa
     7501 attccaccta caattttccc tgagaaattt cagctaagta actatcaaca agtacttgat
     7561 ttattaccat tcatggcttt ttatatcaat acatttttaa tggttgttgg acgagtgatt
     7621 ggctcggttt tattttcagc aatggctgca tatgcttgtg cgcgtctaag ttttccaggg
     7681 aaaaatatcc ttttcagctt agtacttatt caaatgatga ttcccggttc catttttatt
     7741 actccacagt atttattagc acaaaaattg ggtattttaa atacaattcc agcattaata
     7801 ttaccagggg tagttagtgc gtttggtacg tttttattaa gacaattttt cttaggttta
     7861 cctgatgaat tggaggaagc ggcctattta gaaggtgcca atatttggac gatctttttc
     7921 aaaattatgt tgccgcttgc taaatcagga ttggttgcac ttggaatatt cacagcgttg
     7981 tttgcattta aagatttaat gtggccattg attgtcaata cttcaattga taagatgcct
     8041 ttagctccgg gattagcaaa cttacaagga caatatgcca cgaacttccc acaattaatg
     8101 gcaggttcgt tattagcaat ttggccaata gttgtgatat tcatcgtttt ccaaagaaaa
     8161 ttcattgaag gaattgctac aacaggtagt aaattataaa actattaatt agtgattgga
     8221 gagtaagtac atgacaaaaa ttacatttat tggtgcagga agtactattt ttgcaaaaaa
     8281 cgtattaggc gatgcgatgt taactccaag tctacaagat gcacatattg cattgtacga
     8341 tattgatgaa aaacgactaa aagaatcaaa tttaatgctt aaaaatatta ataaaaattc
     8401 gaatgaaaat cgtgctgata ttgtttctta ttatgatcgc aaagaagcgt taagagaggc
     8461 tgattttgtt atcaatgcta ttcaagtggg tggttataaa ccttcaacag tgattgattt
     8521 tgatattcct aaaaaatatg ggttgcgaca aactattggt gacaccatgg ggattggtgg
     8581 gatttttaga accctacgga cactaccagt aatgttagac tttgctaaag aaatggaaga
     8641 agtttgtcca aatgcttggt tattgaacta tacaaatccg atggcgatgt taaccatggg
     8701 tgttttaaaa gcgacgagta ttaagactgt tggtttatgt catagcgttc aagtatgcgt
     8761 accagaattg tttgagcatt taggaatagc cgatcaatat gacttaaatg actttcaatg
     8821 gaaaattgca ggcattaatc atatggcatt cttgttggaa attacaaaaa atggggaaga
     8881 tttctatcca acaattcgtc agttagctgc agaaaaacca aatccacatc cagattctgt
     8941 acgttttgaa ttgttgaaac gttttggtta ctatattaca gaatcaagtg aacacaatgc
     9001 agaataccat ccatacttta ttaaatcaaa atacccagaa ttgattgacg aattagggat
     9061 tccgttggat gaatacttac gtcgttgtga acaacaaatt aatgattggg aaagcatgcg
     9121 agaggacatt gtaaataatg gggagttaac acatacgaga agccgtgaat atgcttcata
     9181 tatcatggat tctattacga caggaactcc gtcagtgatt gcaggaaatg tcttaaataa
     9241 aggcttaatc acaaacttac cgacagatgt ttgtgtggaa gtaccttgtt tagtcgataa
     9301 aaatggcgtg caaccaacct atgtcggtga attgccaaca caattagctg ctttaaatcg
     9361 gacgaatatc aatgttcaag aactgacagt agaagccgct ttaacactag ataaatcaaa
     9421 aatttatcaa gcagcgtatt tagatccaca tctatcatct gaattgtctt taacagatat
     9481 caaaaattta gtggatgatt taatcgaagc acatggggac tatttacctg aatataatta
     9541 aaaagaagct gaaagagtaa ggaattttca attccttact cttttcttag ttaaatttag
     9601 taaaaatatt gacaaaacac ttagtaaaca gttacattag tgttaacaaa aagaaaacgc
     9661 attcaaattg tataaggagg agcataaaat gaagtggtct aaaaaaatta ttttagcttg
     9721 tatagcacta ggagcaacga tctctttgac tgcttgtgga aaaaatcagg aagatgcaag
     9781 cggtaaggta gaaatagagt tttttaatca aaaaaaagaa atgactcaaa cgattcagga
     9841 gattgctaaa gattttgaag caaaaaaccc tgatattcat gtaaaagttg tggatgtacc
     9901 gaatgctggt gaagtaatta aaacacgtat gcttgcaggc gatgtaccag atgtcattaa
     9961 tctttatcct caaagtatag aattgaaaga atgggcgaaa gctggttatt tagaggactt
    10021 aaccgaagaa ccgtatttag agaatatcaa aaatggatat gcacaacgtt ttgctattga
    10081 ggatcgtgta tatagcatac cattaaccgc taatgtctat ggtttttact ataacaaaac
    10141 tgcttttgag gaaatgggga ttcaagcacc agaaacttgg gcagaatttg aaaaaatagt
    10201 agctgagatt aaagatcaaa acaaagtacc gtttgcgatt gcaggagcag aaggctggac
    10261 tttaaatggt tatcaccaac tagctatagc gactgctgct ggtggtgaag aggaagcgaa
    10321 caatgtttgg cgtttctcag aagttaatgg aattaatgct gaaagcaaag aaatgcaaaa
    10381 agattttaat cgattagatt tgttaagaga accaaaagct ttgcaaaaca actggcaagg
    10441 tgccggttat aacgatacag tagttacttt tacaaaaggt gatgcgttga ttatgccgaa
    10501 tggttcatgg gcaatgccga tgattcattc acaaaatcca gattttgaag taggaacttt
    10561 tccttttcca gcagatgaag caggtcaaag tctaacgatt ggcgcaggag acctagcctt
    10621 atcagtttct gcaacttctc aacataaaga agaagccaaa cgttttgtag aatatatgac
    10681 aacacctgaa gcgatgcaga aatattatga tgtagatggt gcaccgtgtg ctgttgaggg
    10741 cgttatcgag aatactcagg attcaccatt aagtggttta actgaacttg cttttactga
    10801 tcgtcattta gtttggttag ccaaagattg gaatagtgaa aatgattttt ataccttaac
    10861 aacaaattat ttacacaatg gcaatcaaaa agccatgatt aacgcattaa atgctttctt
    10921 taatccaatg aaagctgacg gaaggtagga aaataaaatg aaaataaaag aaattttaaa
    10981 tcgttactgg gcaatcattt ttgtagcaat tccaattatt ttgcagatga tttttttcta
    11041 ctttccaatg gttcaaggtg ctttttacag tttaactaat tggactggtc tgacttataa
    11101 ttttgatttt gtgggtttga acaattataa gattcttttt tctgatccga agtttatcaa
    11161 atcgattggc ttcactttaa ttgtgacgct ttgcatgatt gctggacaaa ttggttttgg
    11221 tattttgatt gcgcgggcgt tgaacgcaaa tattaaaggg aaaacattct ttagagcatg
    11281 gttctttttt ccagcggtct tatcgggcgt ggttgtttct ttagtattta aacaaatttt
    11341 taactatggt ttaccaaaaa ttggtagtat gttaggcatt gaatttttac aaaccagtct
    11401 tttaggaaca acatggggag cgatttttgc agtaattttt gttttacttt ggcaaggaat
    11461 tgccatgcca gtcatcattt tcttagcggg ttttcaaagt attccacaag aaattattga
    11521 agctgctaga atggatgggg cgaatgataa gcaaattttt tggaatattg aaatcaccta
    11581 cttattacca agtatttcaa tggtatttat tatggcatta aaaggtggct taacagcatt
    11641 tgatcaaatt tttgctttaa ctggtggtgg accaagcgat gccacaacat ctttggggtt
    11701 acttgtttat aattatgctt ttaaaaacaa tcagtttggt tatgcgaatg ctattgcttt
    11761 agtcttattt atcttaattg ggattgtttc actggcacaa ttaaaattat cgaaaaaatt
    11821 tgaaatctag gaggtttccc aatgaaaaca tcaaaagaaa aaaatatttc taaatacttg
    11881 cttttattat tagggggctt catgatttta gttcccttag tttcaacgct attcggttca
    11941 tttaaaacaa cacccgatat tatgaataat ttctttcgtt ttcctaatcc agcgactttg
    12001 gaaaatttcc gaagattgtt agcggatgga attggtcatt acttttggaa ttcggcaatc
    12061 atcacaatca ttgcagtagt tgctgtgaca gcaattattc ctatggctgc ttattcacta
    12121 gcaagaaaaa tgactcgtgg gaaagcatac ggattgatgt atttattttt aattttagga
    12181 atttttgttc ctttccaagt aattatgatt ccgattaccg ttatgatgag taaaattggt
    12241 ttagcaaata tgtggggatt aatcttactc tatctcactt atgccgtacc acagacacta
    12301 tttttatatg ttggttttat taaactaaat gtgcctatca gtctggatga ggctgctgaa
    12361 attgatgggg ctagtcactt tacaacttat ttcaaaattg ttttcccaat gttaaaacca
    12421 atgcatgcga cgactattat tcttaatgct ctatggtttt ggaatgattt catgctacca
    12481 ttattaattt taaatcgtga ttctaatatg tggacactac cattgtttca atataattat
    12541 acgggacaat attttaacga ctatggtcca agttttgctt cctatacagt aggaattgtc
    12601 acgattacac tagtttatct tgctttccaa agaagtatta tagctggaat gagcaatgga
    12661 gcagtgaaat aaccaataaa ttattctcat tataa
//
LOCUS       DAJSKS010000078        12601 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJSKS010000078 DAJSKS010000000
VERSION     DAJSKS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.080"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(324..893)
                     /locus_tag="ON396_001810"
     CDS             complement(324..893)
                     /locus_tag="ON396_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR3113891.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="ON396_001811"
     CDS             complement(967..2256)
                     /locus_tag="ON396_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113892.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="ON396_001812"
     CDS             2610..3458
                     /locus_tag="ON396_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR3113893.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3472..4545
                     /locus_tag="ON396_001813"
     CDS             3472..4545
                     /locus_tag="ON396_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HCR3113894.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="ON396_001814"
     CDS             4535..6706
                     /locus_tag="ON396_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HCR3113895.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8974)
                     /gene="copB"
                     /locus_tag="ON396_001815"
     CDS             complement(6818..8974)
                     /gene="copB"
                     /locus_tag="ON396_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HCR3113896.1"
                     /translation="MHMGHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHMGHNHG
                     DIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGMEFPFQ
                     VTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYIYSVYS
                     FIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPESVKRL
                     TKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGIKKQAN
                     DKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAKWLFYV
                     ALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAAKNGLL
                     LKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGALEANAN
                     HPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLGLNVEQ
                     ALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVMLTGDNK
                     NAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLARASIGM
                     AIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILAIPLAA
                     GILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9117..11303)
                     /locus_tag="ON396_001816"
     CDS             complement(9117..11303)
                     /locus_tag="ON396_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCR3113897.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11303..11512)
                     /gene="copZ"
                     /locus_tag="ON396_001817"
     CDS             complement(11303..11512)
                     /gene="copZ"
                     /locus_tag="ON396_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCR3113898.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11525..11965)
                     /locus_tag="ON396_001818"
     CDS             complement(11525..11965)
                     /locus_tag="ON396_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HCR3113899.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12039..12512)
                     /locus_tag="ON396_001819"
     CDS             complement(12039..12512)
                     /locus_tag="ON396_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HCR3113900.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
BASE COUNT         3537 a         2653 c         2235 g         4176 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgtcccatgt gcatggaatg gtccatctgg ctatggtcat
     9001 gtttcatctc catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt
     9061 tatgttcatg ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac
     9121 tttttcatgt gccgatttaa gctcagggag ttcaacaata cactgacgga actaaacgcc
     9181 atcgctcctc ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc
     9241 gtattataga caaaagccca gaaaagattt tgtttgattt ttctcaatgt caccctagac
     9301 aactcgattg tttgtgcaat ggatgccaaa ctgctactca tcagcgtcac atcggctgtt
     9361 tccatcgcga tatccgttcc acttcccatt gccataccaa catcagctaa tgctaaagcc
     9421 ggtgcatcat tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt
     9481 tctacgtagg ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata
     9541 ccaacttgtt taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca
     9601 tctaatcctt tattctggag ctgcttgatt gcttgtttgg cttctagttt gatttgatca
     9661 gccacggcaa tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc
     9721 tgctgttcta attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt
     9781 aatcgtttac gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca
     9841 cctgggtgag ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat
     9901 tccacgattg ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat
     9961 aggacttctt tagaaccgac tagatctgta actttgggct ttccttctgt aatcgtccca
    10021 gttttatcca aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta
    10081 attaaaattc catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct
    10141 aaacctaatg cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt
    10201 tgccaatcac cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg
    10261 atcggaacaa aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct
    10321 tgagcatctt ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgtg
    10381 acttccatgt gtagcagacc attcgtattg attgtcccgc caaaaagcgt gtcatccgga
    10441 ttcttttcta caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct
    10501 gaaataattc gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca
    10561 acgactactt cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt
    10621 tttgtttgaa gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt
    10681 tccaaatatt tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga
    10741 tcttgggggt gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt
    10801 ccaattgcta ccaatacatc catatttggt gctttcgact tgattgcatg gtacgcacct
    10861 ttataaaaac gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt
    10921 ggaaaatgaa aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac
    10981 aacatcaacg gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa
    11041 tacgcttgct tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca
    11101 tagccgatgt tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca
    11161 cttgcttttt cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc
    11221 tctttttcga tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc
    11281 attttggttt ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg
    11341 cttgacaaat cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt
    11401 ttttcaaatt tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc
    11461 ttgctgcaca atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt
    11521 cctcctattg atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat
    11581 ctactggttg cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag
    11641 tggcttcttc tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa
    11701 aaagattttc cgtcgcactg cgaactgttt ccatctccga gacagctgga tgataaatga
    11761 atttttttcc ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag
    11821 ttttgatcgt tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg
    11881 ctgtcgtctg tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactaatct
    11941 ttactggcgt tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt
    12001 aacttatgaa tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt
    12061 ttcaatttag ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt
    12121 cttgcattag ctacttggat cagctgctgt atttttggtt cattgtttcc ttcaaaatgc
    12181 cagataaaag ataaaaattc taagtatttc ctgtttagtt tctccgaaaa tgattcaggc
    12241 atatctggcc gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag
    12301 atggcgagat aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc
    12361 tctaacgtag aagtataatt tccttcaacg atccatgacg gattagatac cataaacgat
    12421 tgctgttctt gcaaaaacca cttttttgcc tgaatgcgga aagccggtga tttctttgat
    12481 cttccttgcg agggacgatt ttcccgaccc actttgtcca atcacgatga tcttcattct
    12541 tctccctcac ttctctttgg aatttggttc gatcatacca cttataacgc cgcaaaaaaa
    12601 c
//
LOCUS       DAJSKS010000079        12444 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJSKS010000079 DAJSKS010000000
VERSION     DAJSKS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.081"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            35..1336
                     /locus_tag="ON396_001820"
     CDS             35..1336
                     /locus_tag="ON396_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR3113901.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(1515..1784)
                     /locus_tag="ON396_001821"
     CDS             complement(1515..1784)
                     /locus_tag="ON396_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3113902.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(1774..2040)
                     /locus_tag="ON396_001822"
     CDS             complement(1774..2040)
                     /locus_tag="ON396_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR3113903.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            complement(2109..2336)
                     /locus_tag="ON396_001823"
     CDS             complement(2109..2336)
                     /locus_tag="ON396_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCR3113904.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            complement(2541..3173)
                     /locus_tag="ON396_001824"
     CDS             complement(2541..3173)
                     /locus_tag="ON396_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3113905.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            complement(3186..5909)
                     /locus_tag="ON396_001825"
     CDS             complement(3186..5909)
                     /locus_tag="ON396_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3113906.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            complement(6045..7010)
                     /locus_tag="ON396_001826"
     CDS             complement(6045..7010)
                     /locus_tag="ON396_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3113907.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            complement(7064..7876)
                     /locus_tag="ON396_001827"
     CDS             complement(7064..7876)
                     /locus_tag="ON396_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3113908.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            complement(7890..8663)
                     /locus_tag="ON396_001828"
     CDS             complement(7890..8663)
                     /locus_tag="ON396_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3113909.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            complement(8677..9147)
                     /locus_tag="ON396_001829"
     CDS             complement(8677..9147)
                     /locus_tag="ON396_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3113910.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            complement(9144..9560)
                     /locus_tag="ON396_001830"
     CDS             complement(9144..9560)
                     /locus_tag="ON396_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR3113911.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            9885..10727
                     /locus_tag="ON396_001831"
     CDS             9885..10727
                     /locus_tag="ON396_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3113912.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            11122..12105
                     /gene="istA"
                     /locus_tag="ON396_001832"
                     /pseudo
     CDS             11122..12105
                     /gene="istA"
                     /locus_tag="ON396_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(12186..>12444)
                     /locus_tag="ON396_001833"
     CDS             complement(12186..>12444)
                     /locus_tag="ON396_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCR3113913.1"
                     /translation="GITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFFSLEKLGCQN
                     FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         3914 a         2440 c         2019 g         4071 t
ORIGIN      
        1 gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc atatcaaact
       61 actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga agagcattaa
      121 gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc gctgtccttt
      181 gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa aatcgctcat
      241 tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac aacgctacca
      301 ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata gaaactgctt
      361 tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac aagcaatgac
      421 gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac tcgcttctct
      481 gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa tggatgaatt
      541 tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg gcgatactgg
      601 aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt attttatgca
      661 atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga atgtttctta
      721 tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc gttttcacat
      781 tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga aaagctttga
      841 tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt ctcggcttct
      901 gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca attttggttg
      961 ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg acgaattacg
     1021 aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag aggcggacac
     1081 tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc attctataaa
     1141 aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc cttattcaaa
     1201 tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg cttatggctt
     1261 tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga ttacctatga
     1321 atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat ctcactctat
     1381 cagtcctatt tgacaaagag ccataaaaaa gcagaaacca accgatagta tcgggtagtt
     1441 tctacctgtg gtattatgca aaattcaaaa gattatacat tgactttcga aactctactt
     1501 cgttccaaaa acggttatct tttatatatt gtacttctat gtccaattga gaatatctgg
     1561 atttcaattt cagaatcaca aatattagcc aatattcgat agtttccaat tcgatagcgc
     1621 cattccccac tcttatttcc agtcaaacct tttccgtgct gacgcgggtc agtcgtgttt
     1681 tctaaattct ttctgatcca ggcgacaatt attttagctt ggtttctgtc catttttttt
     1741 aattgcttct gggcttgcgg catgtagatg actttataat tcatttagca gtttctccat
     1801 ttcctcttga gaaataccac tctctttttt ctctttggaa acttcaattg cactccgtaa
     1861 agtccgtaga tcgatttcat cttcgatttt atccagtaca gcatttcgat aaagttcggt
     1921 caatgaagaa ttgtgtgctt ttgcatattc taataccagt tctttttcat cgtcgggaat
     1981 cctaaatgta atggttgatt gagacatgat tctacctccg ttcatcattt gtactaacat
     2041 tgtactaaat aaaatccttt ttgtcaatac aacgttagta caaacaagat ttgcatcata
     2101 tccattattc aaattctttg agatagcacg gtatcgcaaa atctctgact gatcgtttta
     2161 agaagctggt cattttgaag cgattttctc caattttcag ggatcacttc acccccgtaa
     2221 agaatacccg caagagcacc agttacggca gcagtagtat cagtatcccc tcctaaattg
     2281 accgcagtca atatggctgc ttgataacta ttcgtatttt ggttctgttg caaaatattc
     2341 tagcaatccc taggatcatt cacatgacct tagggattgc gaacaaaata aaaaacttaa
     2401 aaagccaatc ccattatcac tgccgaagca ttttcgtaaa aggtgttacc agcacctttt
     2461 actacacctg aattatagca agtttttttg gttagtcaaa atagctagca cagatatata
     2521 gccttatttc cattaattca ctaacctttt tctaaaacaa tttgttccag ttcttgtaga
     2581 ctttcaattt gtatgtctgc ttcactctga tcgatttgca ttggttgttt tagtgccgcc
     2641 gtatagatat ttgctgcctt tgctgattta ataccaatcg ttgaatcctc tactgcaata
     2701 aagaagtcac agtctaattg ctgtttcgct tttaaatata tttcgggatg tggtttgctt
     2761 tgttttaatg actcaccact tataacaaag gaaaaatatt cagtaagttc acattgaatg
     2821 agcatactct ctatctcgac agctggagaa gaagatgcca gtgccaatgg tatatcccgc
     2881 ttcttgagga agtcaagtaa cggtttcact tcttttcgta aagctttagc gaaatcaatt
     2941 ggatgcgttt ttcgataatc tacatatttc ggataaagtt cctctcttaa tgatttgtcc
     3001 tcaggaacta atgtttccca tattgcagct tctgttttcc ccacataatc atcaatctgg
     3061 tttgatcctg gatctatctc cagttcttga aagaagttca ttcttctctc aaaataaaat
     3121 gtctcgctat ctatgagtac cccatccata tcaaaaatta ctcccattgt cattcgaatt
     3181 cactcctatc cttgaacaat atcgctcaat aatcgcaact ctaaatctgg tatttcaatt
     3241 gtgtattccc tttcaatctc aaaaaaagcg ctatgaattt tatccattat ttctccattt
     3301 gcttgcatat attcttcaat gtcctcttgc tcatccacac tttctttacg tacaattcgt
     3361 tcaatcatac aactacagtg aaggtacaac aaaaacagct gattattaga aaaatcaaaa
     3421 tttaataact tcgataattc tttaattgct ttttgtacaa aagatagcac cttatctgga
     3481 tccaaaatgg ttaaattttc gacaatttta ctaattgaaa aattgaaaat taattctttt
     3541 tgtaaatcgt tcacttcaat agtcagctct ttttccaagt aatgaacaaa ttcgccaatg
     3601 cttcccccat taatcaggcg atctaatcca atatatggaa tctgattaat tttgggatct
     3661 tctgtaccta caatcatgat gacatcgtat aattgaaaag gagtctcaaa attcttgttt
     3721 tctaacagct ttttgtaatc atacggaata attactaatt ctctaactgc atctttcaag
     3781 catttcgtta aaatctcctg aatctgggtc gcactaccaa tccctgtata acaacaagta
     3841 atgatggctt tctttttctt tttcttggga agtagtaact gctttttgaa agtaagactt
     3901 gtttcaattt ctgctcctat ttcggtgata cttttattgt ttaatacttg ttggccgact
     3961 tccaataata acggtgtatt gatatgatca atgattaaca gtggtccatt tagatgattc
     4021 actaataaac cgcctagttg gtttagtgaa cccatatcta ccaataaaat caagcctgaa
     4081 tctgttcgat aatctccaag gaattgaatc aattttgctt caattttctc taatgtaata
     4141 tctaaaggca tatcataagc ttgaaaaacg ttcttattca gcatacggtt ggcaacattc
     4201 gccatactac tagctgtaga ataaccatga gctagcacaa tactcttaat acctgtttta
     4261 acattcgtga cattcaaact ataaaaatat agcgtaataa agaaaatgtc ctcttcatac
     4321 acaggttgat ctaaacactc agataaaagg cttaagattt tttttgcata ccaataaggg
     4381 gtttcaagaa cattagcaaa aaattcgtta agctgatctt tggttttctt ttcttgaaga
     4441 ctattttcaa aaatcacttg attatccttg acatatagat agttggccaa agcgactacc
     4501 cggttaccat cttgttcaaa cccgtagctt tctcgcataa aatcaatact ttgacgaata
     4561 tgatagttta gaatggcgta taaagactgc tctttttcgt aatcctcttg aaaaataagc
     4621 gtatccatta cttgtgttac cttggtaacc atttcttcta aaaatagttt aatatccttg
     4681 ttattttcaa tcacttcaga gaatccagta agtaattggt caaaaaattg aacaaaaagc
     4741 tgattgtttc tttctagttg cacttgtcgt gtttcttctg gatagtagat tcgatctgct
     4801 cttttctttc tgggtttatt tagttgctcc ttgaatttat aactgtactc taaaggcata
     4861 tccaataatt taacttgaat tgtttgctga ctcttctggt ggatatatgc gtttccgcaa
     4921 gcatacttga taatattttt aatattcccc acgttacctt caaaatcact attcattaag
     4981 aaatgaatca atgcatcaga aacagatagt tccttagcta aaatttggct ctcagaaaca
     5041 aaaaattcat caatcaatga cagacgttca aacgacggac gttgttgaaa tctaggtaga
     5101 ttaacaataa ctggcaatct cctaagaaaa gttggtaaga aagtcgtata tatattttct
     5161 gttgttgcaa agactaatcg aacatcggct tttttccgaa tactattatc ccccatagga
     5221 gagaattcac ccgtatccat aaatgtaaat aatttctctt gcccttcttc agataagcga
     5281 tgcacttcat ctaaaaacaa tactcctcca tcggcttttt ctagcaaacc agtatgctcc
     5341 tggcttgctc cggtaaatgc tcctttcgtg tagccaaata aagcagagga tagtaactct
     5401 ggattgctca catattgtgc acaattataa tctatgaaag gtgccgatgt gtgaatgacc
     5461 ttttcttcta ctgcatattc atatatttta ttcgctagaa aggttttccc tgatccacta
     5521 gcgccgatca ataataaagg caaccctttt ccaggatata agactgccgt tttaatttga
     5581 tcaattgctt ctttcaaact tcctttagag ccaattactt gatctaatgc gggtttttga
     5641 gaaattgtta acaagctttc aattgattct atttcggagt tattcagtgt gatatgtagt
     5701 tcttcaagag ctgttctatg taaaaataaa accgggtaac tttttacttt tatgaaaagc
     5761 ccttcacgaa caccttcatt taagtagcta ctgattgtac ttctattcaa ctgtagagct
     5821 tgcgaaatac tgcgagctga ataattatct gaaggcgtag atttcaaaag caatgttagt
     5881 tgataaagat agtcatttaa tcgctgcatc tgccacctgc tttcacaata tgtactagtt
     5941 attttaccat atgtactagt tattttacca tatgtataaa atcacattga agaaaacaag
     6001 gtggcttaat ttctcaagcc accttattta gtttgatcta ttttttaata agttagtttc
     6061 cacatataac gtctcttagt taatggatgt tctcttgctt ctgacaattc ttctgcataa
     6121 actctaaata ctgcagtaat cacgaatggg ttaaagtagt ctaagacttc tttagcaata
     6181 tgtgaagaca agccataatc caaggcatct acaacttctg ttttcgtatc aaagcgttca
     6241 aggaaagtta atgctcgagc atctatattt ctagtttttc catcattcat taataatata
     6301 aacggtacgt ctttatccac aatttcgaat ggtccgtgga aaaattctcc tgaatggaag
     6361 gtaccagaat taatccattg catttccatc atcaagcaaa ttgatgttga gtatgctact
     6421 tcatgtgtag caccactact cattacataa ataacttttt catctttaaa gcgttcagca
     6481 aatttcttcg catctttacg agctgattct gcactttttt gggcaagatc atagatagta
     6541 gcaaatcctt cttgcattgc ttcataatgg tcataacctt ctacttgatg taaaagttct
     6601 actgccaatt ctaaacagta gcctgttttt tctaatttag cagcatagtt ctctgcaaat
     6661 cgatgataaa ttacataatc tgcatctttt gttaacggtg agtcaatgct tcgtgttaga
     6721 ctaataactg tagcaccttt ttctttagct actgcgttag cttcaactgt ttctggagtc
     6781 gtacctccta aagaagctga aataacgaca gttgactcat ctacagatgc tggtgttgca
     6841 tagttaaatt cgctggctgt gtaatgacta atccgtaact gcttcgcatt ttggtctaga
     6901 aaatactttg caggataaag gtccgctttt gaagcgccac agccaacaaa tacgattgat
     6961 tttacctctt ttttcgctac gatttcttta acaatttctt ttgaattcat caattgaatt
     7021 cctccttatt tatattattc aaccaaattt attggttatt cccttattgt aagataccta
     7081 acaaactaaa aataattcct ccgactaaac agattgccaa catccatcct gttttgacct
     7141 ttttctgcaa taaccagtac atgaagaagg ttaatcctaa tggtaaaaat tgtggcataa
     7201 tactatctaa tacatcttgt aagacaaaag ctttacccat ttgaattggt gttgtaatcc
     7261 cgatcatgct ggcaatcatc gaaccaatga ccatcaaccc cacaatggag caaacataca
     7321 ttactttatc catcaaacct tcttgataca gtttagacaa gtatttattc cccatgttat
     7381 agccaatttt cccaccatag taagtaacta aaaaggaagg tacaaaggaa attaacattg
     7441 ctagaattgg acctaaaata ctaccttgtt gggctaaatt aattcctagt ccaaaggcta
     7501 atacccgaat cgttccttga aagaaagaat caccaacacc agatagcggt cccatcagag
     7561 aagtcttcac tgcactgatt gattccggat taaagttttc ttggtcttcg tagtattgct
     7621 cctccataga agcagttaac cctaacacaa atgcacttgt ttgaggggta cagttgtagt
     7681 acatcatatg ccgctcgtaa gcttctttct tcattgcttc atcttctgga ttcttgtaaa
     7741 tgttatctag aattggtgca attccgtaca taaaacccat gttcatttgt cgttcatagt
     7801 tccatgaggc ttgaattgcc catgaacgcc ataagaactg ccgatatttc catgcatttt
     7861 gatctttctc tttcacttaa tctacctcct tataagtcat ctaatggatc tggctcattt
     7921 tggttaccat ttgagttttt accttgaact tgaaccatga caaacgcaac aatacatgcg
     7981 aaggctgcaa taccaatgat tcccaatcca gaataagcag taattaaaaa tccaagtaat
     8041 aaaaatggca ccattttttt cgttaacatc gtttgtaata acaacgcaaa cccatatgct
     8101 ggtaaaattt tacttgcaac cgttaagcca tttgttagcc actcaggaat cgcatcaaca
     8161 atctgagaaa caagattcgt tccaaaataa attgctagaa aaataggaat aaaatacatg
     8221 agagcatata atcccgagcc ataaagtata tgaattctcc ttgcttggct aaattttcct
     8281 tgttcgataa agcgatccgc aacgtgagtc aaatttgaca aaatcgtccg cattaagata
     8341 cctagtattt gacctaacac tgaaattgga atggcaatcg ttaatgctgc ttctgcattc
     8401 gcatttgata aaatggcaaa ggctgtagca ataattgatg ccatattcat atcaggtggt
     8461 gaagcagctc cgatatttac taatcctaag gacatcaatt ccaaagaggc tccaacaatt
     8521 aatccttttt ccatgtctcc aagtaccaaa ccaacaagcg tactgacaat caacggtcgt
     8581 tcaaaattca ttcttcctaa aagccttgag tctaaaatac aaaaaacacc gattaaccct
     8641 aaaccaattg actgaacgag cattttcatt cctcctttat aacattgtat tcaaatcttc
     8701 ttttttagaa gttggtacct gttgaataaa ttgtttaatg ccaagagctt ttaatttttt
     8761 ggcatcttct tgctcagatt cattcaagaa aatggcatta ctaaattgct tggaaccttc
     8821 ttttttgatt atccctccat agttgatttc ttgtacttta ggataggctt cacaaaattg
     8881 acgaatttct tttgtattac caaaaataag catgatattt tcgttaagtg tttcgatttt
     8941 aggcattttt tctagtaact gttcaactga aaaaattgtt aatttaatgc cagtcggttt
     9001 agataaattc aaagacattt ttttgaattc atcattcgct gcctctttat tggcaataat
     9061 aatacgattg atatttaatg atttactcca agaaaatact acctgtccgt gcaacaaacg
     9121 atggtcaata cgtactaact taatcatagg tcatctcctc cagtaagttg ttctactaat
     9181 aattttctac aatcaatcac tgattgttga ctctctttaa taagcttttc tagctcactt
     9241 tccccaattt ttccattata ggttgcaata gaaataacta aaggtaagtt cattccagta
     9301 attaattgca atccctcata ctctttaata atagtgagca tatcattatt cacactacca
     9361 cctagaatat cagttaaaat gtaggtttct tctatatcta ttgtggctaa aagttttttt
     9421 atttgtacca aaatagattc atcttcatct ctaaatgctg acaaggcatg aattggtaca
     9481 ctatctccaa taatcattgt cgctgtttgc tttaatcctt gagcaaattc cccatggctg
     9541 gcaagaatta tagttgccat tttcattcct cctatcctta cgcttttcta taagcaagat
     9601 gcgtgccaat tttaggaaac gctgttaatt aaaggttttt ctatatcatg ccaacacttt
     9661 tctttcccta aattatagtg ttgatgaaag tgtgggtatt taaaggtgtc taattaaatg
     9721 atcactgtaa ttggaattta gctttgcccc aatcccccgc cacatcctaa gaaattttct
     9781 tccacatacg aaataaagcg taaaaagcgt ccattaatat ttttttcggt aattacaaca
     9841 aaaaatagaa cttaatgtgc tacaatatat gaatacacgt tcatgtgaaa ggagatgtta
     9901 tagtggaaat caaggtttta tttgaaaact caagaataaa tttgaacttt aaaagtggac
     9961 atgggttatc tctcttaatt aactactcag ggaagaaata tctcttcgat gctggcgcta
    10021 ataaaaaatt agtctataat gcgactaaaa tgggggaaac tttgaatgat ctggaggcag
    10081 tgattatttc acacggtcat tctgaccaca ccggcgggtt aagatattta aaatcaggaa
    10141 acgcccctat ttatacagct gaaggtgtaa ctttacccca ttatttaaag attttaacca
    10201 cgtttctgta tgttggtatt cctaaaaatg aaatccttaa aatgcatgag aatttacata
    10261 caataaaaac gaaaacagaa cttgctccga gcttccatat catcccccta ggcaaagcaa
    10321 actcgaccac caagaattta tacaaaaaga tggatgataa atttgtttat gatgattttt
    10381 cagatgaatt attggtcgta attgagcaag aagacggtct gattgtcttt actggctgta
    10441 gtcatcatgg cattatcgat atgatggaaa ttgctcttga aacatttccg actaaaccca
    10501 taaaatacct aattggtggc ttccatatga ttggcatacc ttatataaat aatttaggtc
    10561 tacctgagca agaggtaatg aaaattggaa ataaattgaa tgactcacga gtagaaaaaa
    10621 tccttacttg ccactgcaca ggttcaaaag ccttcaacat attaaaaaca cagttaggaa
    10681 acaagctaga agctattaaa actggacagc acttagagat aagttaaaag agaaactgat
    10741 aggactgtcg agaggacaaa agctatatga aataccccct ctaatgtttg ctggcgataa
    10801 cacaaaaggc agaccttctg tgaatagggc tacctttttg tgttttttca ctaactttca
    10861 atatcttggt cattcaagga aaatcaccta tatttctgtg tgggtttact tccttattaa
    10921 atcgagtcat ctatttcgtt tattcacttt ttcaagtgga cattcattaa gctgtttatg
    10981 gcagaataaa agtacataca agtgcaacga aaaactacac aactccgcaa atcgattaag
    11041 ttaagataaa aagccattgc gggcaacctg aaactcctct aaagttatag tctcgacact
    11101 gtaactggag gtaagaaagg tatgctcaca atgaccgata ttaatactat cagaaattta
    11161 cgaaataatc atgataaatc cattaatcat attgcaaagg agctagatgt aaattggcgc
    11221 acggctaaaa aatatgcaga tgatgatttg ctgccgcaac ctaaagcacg caaaaagact
    11281 ggtatgatgt atacagaaaa gtggggaggc atcgtttccc tatggctttc agaagatgct
    11341 aagctgccaa ataaaaagcg taggactgct gaagcaatga ctactgcttt aaaagccgat
    11401 ggctttccag gatcctaccg aacagtctgt gtattcattc aagattggaa agcgacacac
    11461 tacttggaaa cactggaaga ccaaggcttt gagcgtcttg aacacccgcc agcagaagcg
    11521 caacttgatt ttggtacgat ggaagtttgt cacgatggag cgtttaaaga tgtgaaggta
    11581 ttagtgatga ctttcccttt tagtaatgca ggattcgccg ttgtactccc cgccgagaat
    11641 caagaatgtt tgttggaagg catgaaagaa ctcttcagcc aatcaggtgg cgttcctcgg
    11701 aaaatacgga ttgataatat gtcaacggcc gttacttaga caaaaacaaa gactgaacaa
    11761 gcggtgctga ctgacggttt cctccgattt tctatgcact atggttttga gacacaggtg
    11821 tgcaatccac gtagcggaaa cgagaaaggt agtatggaaa acaaagtcgg ctatgtacgg
    11881 tacaacttct tttcatctac accgattatg aaggatttcg ccagtttcaa cgaggcatta
    11941 gcggtggagc tgtcacacga tcgagagcga atccattacg aaaaacatgt cattatcaat
    12001 gatctatgga acacagaaaa aacagatttg ctagctttgc cggacaccga ttacccagta
    12061 tttaaagaaa tcgaagtcaa agccaataag tacaacgaaa ttaaacaaaa caaaataaca
    12121 aatttatcgt agttcaatat acttgataga aaaactattt attttacccg tggtgctagt
    12181 taattttaat atcctaaaga atatctcaaa gttccttcaa aacgtcgttg tgctcgactt
    12241 aatagaacac tgtaagccag taatatgctt tcaaatctac tctctagccc tttaacagaa
    12301 cgtgagttga aattttgaca acctaatttt tctaaggaaa aaaaaacagt ctcaactgtt
    12361 tttctttgtt ttccaagtaa gctgtggtct agtttttctg atttatccat gtttttacgt
    12421 ggcggaacgg atatagtaat gccc
//
LOCUS       DAJSKS010000080        12176 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJSKS010000080 DAJSKS010000000
VERSION     DAJSKS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.082"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(138..302)
                     /locus_tag="ON396_001834"
     CDS             complement(138..302)
                     /locus_tag="ON396_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113914.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            597..1925
                     /locus_tag="ON396_001835"
     CDS             597..1925
                     /locus_tag="ON396_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HCR3113915.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2127..2960
                     /locus_tag="ON396_001836"
     CDS             2127..2960
                     /locus_tag="ON396_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR3113916.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2976..3713
                     /locus_tag="ON396_001837"
     CDS             2976..3713
                     /locus_tag="ON396_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HCR3113917.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3868..4836)
                     /locus_tag="ON396_001838"
     CDS             complement(3868..4836)
                     /locus_tag="ON396_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR3113918.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5068..5901
                     /locus_tag="ON396_001839"
     CDS             5068..5901
                     /locus_tag="ON396_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR3113919.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5935..6774
                     /locus_tag="ON396_001840"
     CDS             5935..6774
                     /locus_tag="ON396_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3113920.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6800..7360
                     /locus_tag="ON396_001841"
     CDS             6800..7360
                     /locus_tag="ON396_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR3113921.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7559..9280
                     /locus_tag="ON396_001842"
     CDS             7559..9280
                     /locus_tag="ON396_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCR3113922.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9445..10587
                     /locus_tag="ON396_001843"
     CDS             9445..10587
                     /locus_tag="ON396_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR3113923.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10603..11814
                     /gene="thiI"
                     /locus_tag="ON396_001844"
     CDS             10603..11814
                     /gene="thiI"
                     /locus_tag="ON396_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR3113924.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4151 a         2042 c         2527 g         3456 t
ORIGIN      
        1 taaataaagc ttatgcaaag aaaaagaaat ccaatcactt gggtttcttt ttcttttatt
       61 attatgatga atcaagcttt ctgtttttgt cacgtatgtt gtaatcttta cgaaagagta
      121 gagttggcta ctcataatca accatacttc ccatcatcgc taaatacgaa ataaatcagc
      181 attcctacac caatcaaagc acaagctgca ccaagaaaag ggatgacatt cccaaagtta
      241 tctgtattca taagtatgcc accacccaca aacaaaaagc cgccagcaat caggtttttc
      301 attttattca tctcctttct tacgttatag tatttccttc tttcaatgag tattttgaaa
      361 gctatcttat tgtatgatgt tcttctaggc ctcttcttta ttatacaggc cattttcgtt
      421 atctcataga aatattcctt tttatctgaa aactattgta taccgtatga catttttctt
      481 catgaagttg taagtatatt ctcattcaaa tgaaaatttc atcatagaag agattgtatt
      541 tctaaattct taagagtaaa atacgaagta taacttggat ttggaaggat gattggatga
      601 aagctattac gcctgatatg acaacaaaat ttaacaaaca gtttcaagca aatatcaaac
      661 agcaggcatt gcagcgggca gttgtgaaga acggaattac tgcttctgct gaaaatgttt
      721 cagcacatgt ggagaataca ccggtatttt ctatagattt ggctacgggg aaagtggcta
      781 atcaaatgca atcggggcgt tgctggatgt ttgctgcact gaacacattc cgtcataaga
      841 tgctgaatca attccattta aaagagtttg aattatcaca aaattacact tttttctggg
      901 ataaatatga aaaggcaaat tatttctatg aaaacatcat tgcaacagga aaagaggcaa
      961 tcgatagccg gaaagtagct ttcttactcg ctactcctca acaagatggc ggccaatggg
     1021 atatgatcgt ttcattattc caaaaatatg gcgttgtgcc taaatcggtg atgccggaaa
     1081 gtgcgaatag ttcaaattct cgagatttga ataactactt gaacaaatta ctgcgtaaag
     1141 atgcagtagt gttacggaaa atggttgctc aaggagaaac attagacgct attgaagaaa
     1201 agaaagaaga aatgctagaa agcatttata acttcttagc tatctcatta ggaacacctc
     1261 caaaagaatt cgattttgag taccgcgatg aagaaaagaa ctatcatctt gaccgaggac
     1321 tgacaccaca aattttttat gataaataca tcggtgtaaa attagatgat tatgttagcg
     1381 tgatcaatgc accaacaaaa gacaaaccat tcaatcgttc ttatacagta gagatgctag
     1441 gaaatgtcgt tggcgggaaa gaagtcaaat atctgaatgt tgatatggaa actttcaaaa
     1501 aattagcgat tgctcagctt gaagaaggag aatctgtttg gtttggttgt gatgtaggtc
     1561 aatcttctac tagaactagt ggcatcatgg ccttagatgc ttattcaatg gatgatctat
     1621 ttgatacgga ttttacaatg actaaagctg aacgtttaga ttttggagaa agtctgatga
     1681 cccatgcgat ggtattgacc ggtgttgact tagtagacgg gcaatcaaca aaatggaaag
     1741 tcgaaaatag ctggggcgaa aaagttggaa agaatggctt ctttgttatg agtgatgcct
     1801 ggatggacga atatacgtac caaatcgttg taagaaaaga tttattgacg aaagagcaat
     1861 taaatgcgtt caatgaagaa ccaattgttc tatctccgtg ggatccaatg ggtgctttag
     1921 catagacgag tatttcaatg aagagtatgt agtaaagata aaaagagctg gcttaagcca
     1981 gctcttttta tctttaaagt ccattccctg ttttttatgg tccctgtgct ataattctaa
     2041 ataaaggata aggattggaa ataatccttt tatcattaaa aatatgatga cgaagtagaa
     2101 taaaattggg agtagggggg aaagtcatgg atatccaaac tgttttatct gatctgaaat
     2161 taaatggtaa agtgattcca gttgttggtg gcgatgttaa tcagacctat cgtataaaaa
     2221 ccgaacatcg tgcttatttt ttaaaaatcc atccgaatgt aaaaaaagga ttttttgaag
     2281 cagaagtaga cggattaaaa gaactgtcag cattcgtacg tgtaccggat acgtatatgc
     2341 taggtgaaac aagtgaaggc gcttatttgt taatggaatg gatcgagccg ggaaaagggg
     2401 atcaacgaga tcttgctgca gctttagcta atctccatca gcaaacagcg ccacaatttg
     2461 gttttcgtaa agataactat ttgggtacac ttgttcagaa aaattctttt gaagaagatt
     2521 ggtggacttt tttctttaaa gatcggcttg aatcccagat ttctttggca gaagaaacga
     2581 atcgatggaa tgtacagcgt caagaaaagt atctgcgatt taaagaacgt gtattgaaat
     2641 cggttgaacc aaaaaagatc actccacgcc tcttgcacgg agacctgtgg agtggtaatg
     2701 tgttctttga ccagcaaggg catccagttt ttgtcgaccc agctgtttct tatgggaatc
     2761 gggaacaaga tattgcaatg agccagttat tcggtggttt ccgaccggaa tttttagatg
     2821 cttatcaaac tatatttcca ttagaaaagg gatggaaaga ccgactgccg atttaccagt
     2881 tatattatct tttggcacat ttgaatatgt ttggagaaag ttacggttcc caagtagatc
     2941 aattactaga aaatttttaa gaggagggcg tgtttttggc aaaaaaatat accaaaaagc
     3001 atgaagtttc atattatgag tgtgacatca accaaacgat gacatttcct tcgatgttag
     3061 gagtagttat caaaacgtct gaagatcaaa gcgaccagtt gggaagaggc agtgaattcg
     3121 tcaatagctt tggacttact tgggtcatca cgaattattc catggagatt aatcgtctcc
     3181 ctagagtcgg tgaagtaatc tcagtaacga ctcaagccat ggagtacaat aagtttttct
     3241 gttatcggaa cttctggatc catgatgaag atgggaaaga attagtcaaa atagaatctg
     3301 ttttcgtatt gatggatttt gtgaatcgaa aaatgagcag tgttaatgaa gagatcattg
     3361 ctccttttga gagtgagaag atcaagaaaa tcaagcgtca ggaaaaaatt gaaaagattg
     3421 attcgggctt catgttgcca tatcgtgttc gtttttatga tatcgatagt aatcagcatg
     3481 ttaataatgc gatgtatttt aattggatca ttgacgtatt aggatatgac tttttgacta
     3541 cgcatgttcc agaaaaagta attattcgat ttgataaaga agtagagtac gggaatgaga
     3601 tcgaaagcca ttttgaacaa atccctatcg aaaatggtgt caaaacaaaa catgaaatcc
     3661 gaatgcaaga acagctgtat tgtgaagcaa atattatatg gaaaaataaa taaaaaagag
     3721 gttgtgacag aagggggcaa cttcaagaaa taagaagaaa tttacgaaaa ttgtttctca
     3781 aatttttgta aattttcgac ttagttttcg aagaagctat ttttgtcaca gcctctcttt
     3841 ttaagtttag ctattagata atgatgttta gttttggata aacgaaatca attcttcacc
     3901 cgacaaacca tagtttacag caatcaatag ttttaaacgt gctttttgac tattaatact
     3961 gttacagaaa ataatcccca ttttttctag ttcttttccc ccgccttcat aatcataaac
     4021 aggctctgcg attccattga aacaacgaga aacaagtact acagggattt tttggacaag
     4081 aagcttttcc aaagcagcta aggcttgtgg agggagattc cccgcaccta aagcttcgat
     4141 taccaaaccg tctactttcg tatgggcgat ggcttccaat aagtcccctt gcatcccagc
     4201 atatgcttta acgatcggga tagtcccatc tacagcggaa atgtccagtc gctttgtttc
     4261 taaaagttcc tgcaagaata aaattcgatt cttagttact aagccgatgg gacccaatgt
     4321 aggtgttcgg aaagtagcaa catttgtggt atgggtcttt gtcacgtagc gtgcagaatg
     4381 gatctcatcg ttcatgacca ccaaaacccc cttatctagc gcttcttcac aacttgcgac
     4441 acgaatcgca ctttcaaaat tataaaggcc gtcacttcca agttcattac ttgaacgcat
     4501 tgccccagtc aaaacaattg gtaatttgtt gccaatcgtt gtatcaagaa aaaaagcagt
     4561 ttcttctaat gtatctgtac cgtgtgtgat caccactccg gatatacctg atgcataagc
     4621 ggacaagata cgttctttta ataccaacat gtgagaaggc atgatgtgtg gtgagggaag
     4681 attgaagata tcttcaacta ataattctac atgcttgggg attgtgattt ctgcatctaa
     4741 taatgggttt gccacatcag gactaacacc gcccgtgagc atgttttctt tcatggcgat
     4801 cgttccgccg gtgtgtagta caagtatttt tttcatttta cagacttcct tacgaataat
     4861 tttgcatagc cgttaaatct tattgtatac tatataaaag agttttgcta tcagtgaaga
     4921 ataacgaata gcctaactta tcataaaaaa gctgacctta ttaaaatgga aaagactcaa
     4981 ccttgacctc ctaatacaat ggagtatctg gttgagtttc ttttacaaga aagcagccaa
     5041 ggaaatcaat cacgaaggtg gccggaaatg atcagaatcg gattaacatc gtttaaagaa
     5101 catgacaaat taactggtaa aaagaataac actctttatg aatatgcagg gtttctaccc
     5161 ttagtggaat tagatactgc ctattatgga attccccgaa agtcctcagt tgagaattgg
     5221 ataagtgaag tacctgaatc atttcgcttt gtagtcaaag tctatcaagg aatcagcggt
     5281 caaggagact ggaagcctta ttatcagacc gaagaagaga tgattgcgca ttttttagat
     5341 gttatgtcgc caatggtaga tgctggaaag atgttttgct ttttagtcca attcccctca
     5401 tcattcaaat gtacgaaaga aaatgtccag tacttgcgca aacttagaaa gtggtttgag
     5461 ggttatcctg tttctataga attgcgggat tattcttggt acagcgatga atttcttgaa
     5521 cgtacaagaa actttatgtc tagagccgct ttttctttag ccatcatcga tgagccacag
     5581 ttgctgaata caacgattcc ttttgatcca tttgtcacca atccgaactt tgctcttttt
     5641 cgattccatg gtaggaatgc gcaaggatgg caagcaaatg ataaagagtg gcgtaaaaaa
     5701 agaacgttat atcgttattc agaaaaagaa ctgcaagaac tagcacatga agtcaaagta
     5761 gttgattcta agacaaaaga ggtaggtgtg atttttaata ataattctgg tggtgatgcg
     5821 gctgaaaatg cattagcact gcaaaaaata ttaaatatca agccagatgg actcaatcca
     5881 acccaaatgg atctgtttta agggcttatt ttattcaaga aaggggcgaa gtctatgatc
     5941 aaagctattt tttttgatat tgacggaaca ttggtcacca atcgttcaaa agcgttggaa
     6001 tcaacaaaac aagcaattga atatgctcgg aaaaacggca ttttatgcgg tgtagcaacg
     6061 gggcgcagcc cagtaaagat caaagaaatc attgatgaat tggaattaga tatgtatgtc
     6121 gtgtataatg gtcaactggt ttttactgca gatcgcacga ttatcgacca cccttttgaa
     6181 caaaaagtat tggaacatat tgttgaattt gcagatgaga accatcgaca aatcgttttt
     6241 ggtgcgagaa atcggttaga tggatcaacg acgatgctat taggacaatc gatttttatc
     6301 aagcgactag tcagcttttt accgcgtaaa ttcccggtac gtctgatgaa aaaaatcctc
     6361 caggttttta gccctcaccg acaaaaggat cgttatgaaa cgttagagat attaaaagaa
     6421 ccaatttatc aatgtatatt gctcagtccg gagtcagaac agaaaaagtt agaacatcgc
     6481 tttcccgagt gtacgtttca gcgttcaaat acgtatactg tagatatcat cccaaaaggt
     6541 ggttcaaaat tactaggcat ccaagctttc gcaaatgctg tcgaaattga aatggaagaa
     6601 attatggcat tcggcgatca ttacaatgat atcgagatgc tgaaaggtgt aggaattgga
     6661 gtggccatgg ggaatgctca gatcgaagta aaacatgctg cagactttgt gactcaatca
     6721 aatgaagaag atgggatata cgaaggactg aaacacttcg gtctgattta ttagtcaatg
     6781 aaagaaggaa aaaatcagta tggaagcaga gacacctttt caaaaagtag aaacattcca
     6841 caaaactttt gatccaagaa gaccagagat acccacggct tttacagaaa atgaagctca
     6901 tacacgtgct gcgttcaaaa tagaagaact tgttgaattt gttgcagcta cttcacctaa
     6961 taaagaacaa ctgcatgaag ctgttatgct gctgcacgaa gcattagata aagcagaaga
     7021 aaaaatactt ggtaaaacac agtggggaag tgatccgctg gttgaacaag tagatgcact
     7081 gacagatatc ctttacttta cttatggttc tttttcatta ctaggaatag tgcctgatcg
     7141 aatattcgaa atcgtccatc aggcaaatat ggggaaatta tttccagatg gaaaacctca
     7201 ttatgaccct gtaacaaata aagtgttgaa acctgaaaac tggcagatgg aatatgctcc
     7261 agaacctaaa atcgcggcag aaatccaacg tcagattcaa gaagcgattg ctaagaagaa
     7321 atccgctgat ataggcatgt gcacaaatga aaaagagtga tcagagtttc actatttttg
     7381 ttatctttat acgatggaaa aacaaaagat tttcgtttaa gttagtgaat gcatgaaaat
     7441 agtatgctat aatctagaat agatgtcttt tgcacgagag acgtattatt tggatttatg
     7501 ccaagcagaa cgccatctgt tgcataaaag atgcatactg aaaacggagg aaccataaat
     7561 gggaaataat gttatcatcg gaatcgtcat tgcaatcatt atagtcgcca ttgtcgtctt
     7621 tttggttagt tacttgatga agaaaaaaaa ccaagatcga ttggatgcct tagaaaaacg
     7681 caaggaagaa ttatttgact tgccagtgat tgaagaagtc gatgatgtta aaaaaatgca
     7741 tctggttggg caaagccaaa atacctttcg tgaatggagt caaaaatgga atgacatatc
     7801 tacttcctcg ttcgctgaac tagaaagtca aattttcgaa gtggaaaacc tcaatgaaag
     7861 ttttcggttc ttaaaaggaa aaaaagcagt tgttgaagca gaagctacaa tggaccaaat
     7921 ggagaaacaa gtgacggaaa tccgtgaagg tctaaaggaa ttgcgggaaa cggaagaacg
     7981 caactcacta gctgttcagc aggcacttga cgtctatgaa gaactgaaag aaaaagtcag
     8041 agaagacgag catgaatacg gaccagctct ttttgaattg caaaaacaga tcaaagtgat
     8101 cgaaaaagaa tttacgcagt ttgtgacttt aaacacttct ggagaccctg ttgaagcccg
     8161 cgaggttttg gataaagcag aaaaacatac gtatgaagtg gaagctctga tgaaaaaaat
     8221 tccaccgctg tatgaggagc tccataccac tttccctgaa caacttgaag aaatcagtga
     8281 tacttacgag aaattgatgg aagaacaata tgtattccca gaagaagatc ttgctgaaga
     8341 tatcgcaaaa gtaagtcgaa gaattgaaaa ttcattagcc aatcttgaaa aaacagaagt
     8401 tgaaacggtc gaatttgaaa accgtgaaac agctgatttg attgattcgt tatatgatat
     8461 cttagaacgg gaaatggaag ctcagagata tgtaaaaact aaccaatcaa cgattgcaga
     8521 gtatatcaaa catacaacaa agaataaccg tcaattattg attgagctgg atcacacatc
     8581 tcaaagctat acattgaacc acaatgaaat tggccgtgta cgaggtttcc aaaccgaagt
     8641 ggaagaaatg gaacgtcaaa atgaacagat gattccacag atccgtcaac acgaaattcc
     8701 ttattcagaa gtgcgcacgt tctataaaac agtcttcaaa gtcttagaag atatcgaaaa
     8761 gcaacaagta gagatcgacg actcattaca tgaattgaga aaaggcgaaa aagaagcgca
     8821 agaaaagatc gatacattcg aattcaaatt gcgcagtttg aaacgttttg tagaaaaaca
     8881 acgtttgcct ggcttgccga atgattacct agaattcttt tttgttgcta ctgaccgtat
     8941 tgaagaacta agtgttgtct tgaataaaat tcgagtaaac atggaagagg tcaatcgttt
     9001 agttgctcta tgtgaagaag atctagaatt attagataag aagacacatg atttagtaga
     9061 tgcagcagca ttaacggagc aaatgctcca atatgcaaat cgttatcgtc atacacacga
     9121 agaagtgaga gaagcaattg ataaaagcta ctatttattc aataaagaat atcactatca
     9181 agaagcatta gacgaaatcg gtacagcatt agagcgagta gaacctggtg ctttcaaacg
     9241 catcgaagat ttttatttta atcatccaga cttagtataa agcaaaaaag ttagtaaacc
     9301 agcaagagca agacggatta ttgtcttgtt cttgtctttt tttgtatatt tgaaaattga
     9361 aacaagtgaa aatttcacct gctcttgaaa gcaccttgga caaccgttat aatagaaaag
     9421 aatttcaaaa aagaggggtt tcttatgata tattttgata acagtgcaac aaccccaatc
     9481 tttccgcaaa gcttagatgc atatgtaaaa accagtcagc ggattattgg gaatccatca
     9541 agcttgcatg atttaggcaa tcaagctaac cggttgttgc agcaagcaag aaagcagatc
     9601 gctgatttga tccatgtaga accagaagaa atctatttta cttcaggcgg aacagaagga
     9661 gataactggg tattaaaagg aacgatgatc gaaaaaagag aatatgggaa tcatatgatc
     9721 atttcaggag tcgaacatcc ggccgtcagc gagacagcag aacaattaaa agaattagga
     9781 atcgaagtaa gtatcgcccc agtggataaa cgcggatttg tacgtgtcga cgaattgaaa
     9841 gaattaataa gaaaagagac agtattagtc tctgttatgg ctgtgaataa tgaagtagga
     9901 tcgattcagc ccatccagga aatcagtgat cttttggatg attttcctaa aattcatttc
     9961 catgtagatg ctgttcaggc gattggaaaa gtaccattct cgcaatggtt gacagaaaga
    10021 gttgattttg caacattttc agctcataaa ttccatggcc cacggggaac tggtttcatt
    10081 tattggaaaa agggtcgtcg tttagcacca ttactgacag gtggagggca agaaagaaat
    10141 cagcgaagtg ggacagaaaa tgttccaggt attgtggcta tggttaaagc tttacgttta
    10201 tcattagaga taaaggaaca aaaacctgag cagacacaac gcttaaaagt agctctaatc
    10261 aatgcgttga agacgtatga gaaagtgacg attttttcag aaggaactga ctatgcaccg
    10321 catattttat gttttgctct aaaaggaatt cgcggcgaag ttctcgtcca tgccttagaa
    10381 gaaaaacaaa tatttgtttc tactactagt gcctgctcct cacgtaaaca tatggcaagc
    10441 agtacattgc acgcaatgca tgtaccaaat gatctggcta catctgctgt tcgaatcagc
    10501 ttagatgaaa acaattcgct tgcagaagtc gagcaattta tgattatttt caagcagctt
    10561 tataagaaat tttctgcaat caattaaaag aggagtgttc ttgtgaaata cagtgaaatt
    10621 atggtccgtt atggggaact ttcgacaaaa ggaaagaatc gaaaaagctt tatcatgcaa
    10681 ctagcacaaa atgtcagaca agctttgaaa gactttccag aaatcaaaat ccatgctgat
    10741 cgggaccgga tgcatctttt attgaatggc gcagacgggc agctagttat tccaaaacta
    10801 gcaaaaatat ttgggatcca aaatttctct ccaagtattc gtgtagaaaa agatgtcgaa
    10861 gttttgaaac aagctgttca agacatcatg aaagaaattt atactgggaa agaaacattt
    10921 aaaattatcg ctaaaagaag cgatcaccaa tttgaattga caagtaatga attgaatcaa
    10981 acattaggta atgctgtttt tgatattttc ccgcatatcc aagttcaaat gcgtcaacca
    11041 gatattccat tacgtgtaga gattcggcgt gatggcgctt atctatcgta tgaaacgatc
    11101 aagggagctg gaggattgcc cgtaggaaca agcggaaaag gaatgcttat gctttctggg
    11161 gggattgatt ctcctgtagc agggtacttt gccatgaagc ggggagtgga aattgaagct
    11221 gttcattttg ctagtccgcc gtatacaagt gaacaagcac tgcaaaaagc aaaagatcta
    11281 acagccaaac tagcacctta tgtgggaacg atccatttta tcgaagtgcc atttaccgag
    11341 attcaagaag aaatcaaaaa gtcagtgcca caaggatatt ggatgacaat tactcgtcga
    11401 atgatgttgc gtttgaccga tgaaattcgc cgtattcgtc atggtttagt cattttgaat
    11461 ggtgaatcat taggtcaagt ggcttcacaa actttgcaaa gtatggtggc tatcaatgaa
    11521 gtaacgaata cgccaatcat tcggcctgta gcgacaatgg ataaacttga aatcattgaa
    11581 gtagcagaac agatcgatac atttaatctt tctatccagc cttttgaaga ttgctgtacg
    11641 atttttgctc ctccacagcc aaaaacacgc cctcaactgg aaaaggtcca tcaatatgaa
    11701 gagcgtttag caatagatga aatgatccaa cgagcactag ctggcttgaa gatggaagag
    11761 atacaagcag aatcgtctaa tgaaaaaact gaagaatttt cagaattgct ataagaggtt
    11821 atgaaaatag tgttttgacc agagtattag agaaacaaaa cgaaaaaata gctccatgta
    11881 ttttaccgtt tttgaactaa tgtcgaagat tagctgattg aataccattt atcaagaagt
    11941 tatggaaaag ccggttcttt tttactaact gaaaaataaa attttattta gagatcaagc
    12001 tacagttgat aaatgatata aaaaagagaa tttcccatga aatagcgagg ttgtgacaga
    12061 agtgggcagc ttcaagaaat aagaaggaat ttacgaaaat tgcttttcaa atttttgtga
    12121 aatttcggct tatttttcga agaagttact tctggaacac cgtgtatcag aggttg
//
LOCUS       DAJSKS010000081        12128 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJSKS010000081 DAJSKS010000000
VERSION     DAJSKS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.083"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(21..578)
                     /gene="frr"
                     /locus_tag="ON396_001845"
     CDS             complement(21..578)
                     /gene="frr"
                     /locus_tag="ON396_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR3113925.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(581..1303)
                     /locus_tag="ON396_001846"
     CDS             complement(581..1303)
                     /locus_tag="ON396_001846"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR3113926.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1439..2320)
                     /locus_tag="ON396_001847"
     CDS             complement(1439..2320)
                     /locus_tag="ON396_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR3113927.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2419..3201)
                     /gene="rpsB"
                     /locus_tag="ON396_001848"
     CDS             complement(2419..3201)
                     /gene="rpsB"
                     /locus_tag="ON396_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR3113928.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3560..4039
                     /locus_tag="ON396_001849"
     CDS             3560..4039
                     /locus_tag="ON396_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCR3113929.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4256..5347
                     /gene="phnW"
                     /locus_tag="ON396_001850"
     CDS             4256..5347
                     /gene="phnW"
                     /locus_tag="ON396_001850"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HCR3113930.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5340..5468
                     /locus_tag="ON396_001851"
     CDS             5340..5468
                     /locus_tag="ON396_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCR3113931.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5472..6017
                     /locus_tag="ON396_001852"
     CDS             5472..6017
                     /locus_tag="ON396_001852"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCR3113932.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6471..8162)
                     /locus_tag="ON396_001853"
     CDS             complement(6471..8162)
                     /locus_tag="ON396_001853"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR3113933.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8582..9529)
                     /gene="arcC"
                     /locus_tag="ON396_001854"
     CDS             complement(8582..9529)
                     /gene="arcC"
                     /locus_tag="ON396_001854"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR3113934.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9644..10663)
                     /gene="argF"
                     /locus_tag="ON396_001855"
     CDS             complement(9644..10663)
                     /gene="argF"
                     /locus_tag="ON396_001855"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR3113935.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10754..11983)
                     /gene="arcA"
                     /locus_tag="ON396_001856"
     CDS             complement(10754..11983)
                     /gene="arcA"
                     /locus_tag="ON396_001856"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCR3113936.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3311 a         2522 c         2193 g         4102 t
ORIGIN      
        1 cctctgtttt tatgatttta ttaaacttcc aataattctt tttctttatc agcagcgatc
       61 ttatctactt ctttgatgct gttatctgtc aatgtttgta cgtctttttc taatccgcgc
      121 aaatcatctt cggtaatatc accatttttt tgttgttttt tatattcatc gatcgcatca
      181 cgacggatgt tacgaatcgc gatttttgca ttttctgctt cttttttcac atctttagct
      241 aattcttttc tgcgttcttc tgtcagttgc gggataacta agcgaatgac atttccatca
      301 ttggttggac tgattccgat atcacttgcc ataatagctt tttcaacatc ttgaagaatg
      361 ctcttgtcaa aaggtgtgat cattaaaaca cgtgcttctg gaatttggat tgatgccatt
      421 tggttcaatg gtgttggcgc accgtagtag ttcacagtaa tacggtccaa aaggctagcg
      481 ttcgcacggc ctgcgcggat ttgcccaagt tcacgttgaa gattttgtgc tgcttttttc
      541 attttttctt ttgcttctgt cataattgca tctgccattt ttatttcccc cttacagttg
      601 ttccgatatt ttcgcctaag attgcacggc gaatatttcc gtgttcattt aaattgaaga
      661 caagcagtgg aatatcatta tccatgctta gtgaacttgc agttgaatcc atgacttgta
      721 atcctttggc aataacttcc agatgagtca gttcttcaaa ttttactgca tttgcatcta
      781 ctttcggatc agcagaataa acaccgtcta cattgttttt agccatcagg ataacatctg
      841 cgccgatttc tgccgcgcgt aatgctgctg ttgtgtctgt cgagaagtac gggttgccgg
      901 taccgcctgc aaagataacg atccgtcctt tttctaaatg acgttctgct ttacggcgaa
      961 tatatggctc tgcgatttga cgcatttcaa tcgaagtttg cacgcgtgtc ggtacaccca
     1021 cgttttccaa cgtatcctgt aaagctaagg cattcataac tgtagctaac atacccatgt
     1081 aatcagcttg agcacgttcc atgcccattt gtgctccgat ttgtccgcgc cagatatttc
     1141 cgccgcccac aacgattgcc atttcaacgc ccagctcgtg aacttctttg atttcttgga
     1201 tgatttcttt gattactggt ggtttgattc caaatcctgc gtccccagct aaagcttctc
     1261 cactcaattt taagactaca cgctgatatt tcggtgtgac catgttcatt cctccaatat
     1321 tttctattgc cattttacca tacttagatg atttcgacac tctaagactg gtttttcttg
     1381 ttttgcttaa aaaaagggag cgcacaaacc gtgcgttccc gtctgttatt caacagaatt
     1441 attttttcac ttggctcata acttcttcta caaagttatc ttcgcgtttt tcgatacctt
     1501 cgccaacttc gaaacgtacg aatgttttaa cagttgcgcc ttttgatgca acgtattttt
     1561 caacagtcat atcaggatct ttaacgaatg gttggtccac caaagcgatt tctgctttga
     1621 atttcttcaa gcgtccttct accatttttt caacgatatt cgctggtttg ccttcgttca
     1681 atgcttgttc tgtaagaaca gttttttcat gttctaattc agcttctgga atttgtgatt
     1741 cgtttacata acgaggattg atagctgcta cgtgcatagc tacgtctctt gcaactgttt
     1801 cgtcagttgt tccatctaga acagtcaata cggcaatacg tccgcccata tgcaagtatc
     1861 caccaaatgc agcattgtcg tctttttcta caacttcaaa gcgacggaag ctgatttttt
     1921 ctccgataac ttgagttgct tcgatcaagt cagattcaat tgtaccttta tctgttttga
     1981 ttttcattgc agcttccatg tctgctggtt tgttttcagc aacaagttct gcaatttctt
     2041 ttactaaatc ttggaacatt tcgtttttag aaacaaagtc tgtttctgag tttacttcaa
     2101 cgattgctgc agtgttacct ttcacagcaa ctgaagccaa tccttcagct gcaatgcggt
     2161 catttttctt cgcagccttt gccattcctt tttcacgtaa aagatcaact gctttttcca
     2221 tgtcgccttc tacttcaact aatgcttttt tcgcatccat cattcctacg ccagtcattt
     2281 cgcgtagttc ttttaccatt ttagctgtaa cgtctgccat gtttaactcc tcctgtgtgt
     2341 ttttcttttt aaaaaaagct gcttcaaagg agagtgaggc ttgtcgccca gcctttgaaa
     2401 cagctccatc attcaaaatt attctgctga agaattgtcg ccttcaacaa catctacgat
     2461 ttcttcgatt gatgttgctg tgttttcttc tgtgaaatct tcttcaacta cttgatcttc
     2521 accttggttt ccttcgatga aagcatcagc catttttgaa gtaatcaatt taacggcacg
     2581 aattgcatcg tcgtttgaag ggattactac gtcgatttca tctggatcgc agtttgtatc
     2641 aaccatagct acgattggga tatttaattt gtgtgcttct tgaacagcaa tacgttcttt
     2701 acgtgggtca acgatgtaca ttacatctgg gattcttggc atatcagcga taccgcctaa
     2761 gaatttttca agacgttcac gttctttgtt caaaccagca acttcttttt taggtagaac
     2821 ttcaaaagtt ccatcttctt ccattgcatt gatttgtttc aaacggctga tacgtttttg
     2881 gatagtatcc cagtttgtca atgttccgcc taaccaacga tggttaacga agtattgtcc
     2941 tgcacgagtt gcttcttctt tgattgcttc ttgtgcttgt ttttttgtac ctacgaataa
     3001 tgctacgccg ccttcttcag caacgctctt catgtaatca taagctgcat ctactaattt
     3061 aactgttttt tgcaagtcaa tgatgtagat tccgtttctt tctgtgaaga tatatttctt
     3121 catttttgga ttccagcgac gagtttggtg accaaagtgt acgccggctt ctagtaattg
     3181 tttcatagaa attactgcca tgtttgtttc ctccgatttg gttttatttt tcctcttctt
     3241 atcttctacc ttgccggcaa actttttcaa gcacctaccg acaatccaat aagaatgtgg
     3301 atttgctgat gcataaagca ccctcagcta ttatacagac aaaagatccg tatcgcaaga
     3361 gcaaatatac ttttctgctc aacattcaat agtttacctg aaagataaaa agaattcaag
     3421 aaaaacagta gatttctctt tgtttcttta ttatcctttt tccataaaat aattttaggt
     3481 ataaaagacg aatattattg ggcaatcctt cttttttttg ttatgataca tgtgaaatct
     3541 ttcgtaaagg agaaagaaaa tgcgcaaagc tcaaagacat ttgctgatta aacaagtcat
     3601 tgaagaattc ccgatccgta cgcaagaaga actattaaac aaacttgggc attatggcgt
     3661 taccgccacg caggcaacga tttctcgaga tatccgtgat ctaaaaatcg tgaaggcacc
     3721 tgatgaaaat ggcgtttccc gcttcgtatt gtttcgcgaa aaaggaactc ctgagactag
     3781 gactgaggaa gaggatcggc tggttcaaat gattgaagac attgtattga aagtcgaacg
     3841 agtccacttt ttgacgatcg tcaatacctt gccagacaat gctcatttat ttgcatctgt
     3901 tttagatgag atcaaacctc cctctattgt cagtacaatt gctggattcg atacgatcat
     3961 ggtcatctcg gaaaatgagg aggacgcaaa acgagtagaa gaatttcttc accatccaac
     4021 tgaagtatca cttttttaat cagagcacgt attcctaata tggaatgcgt gtttttttat
     4081 tttcaatatt ttccttattg aataaacctt tacactcttt acaaaaatac atcatttttt
     4141 attcaaattt ttcaacacct tgattgaatc acaataatca tcatttatat tggatgttaa
     4201 caagagcagc ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa atcatatgta
     4261 taaattatta acttctggac cattgacgac ttctgaaaca gtgaaaaatg ctatgctgat
     4321 cgatcattgt acttgggatc aagaatacaa agaaattaca caatggataa gaaaaaaatt
     4381 attggatctt gctcaagtat cggaaggaat atacacaacg gtcttaatgc aaggtagcag
     4441 ctccttcggg gtagaatcgg tattaagttc tgtgatcagg ccagaagata ctgtactgat
     4501 ttgttcaaac ggcgcatata gacaacgaat gatcaacatg tgcgaatgtc tgaaactgaa
     4561 ctataccgtt tatgaggtat cggaacatca aatcctccaa gctcatgtga ttacacagct
     4621 gattgaagag aatccgtcta tctccgcact agcaatggtc catagcgaga ctacttccgg
     4681 tattttgaat aatttggaag agattgctgc tgtcacacaa gaaagagatc ttttgtttat
     4741 agtggacgcg atgtcaagct ttggtgggat accgatttgt gtagccgaac tagggattga
     4801 ttttttgatt tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt cttttgtact
     4861 tgctaataaa gcggaactgg aaaaacgaaa aaatgttagc cgaagtttgt cacttgatct
     4921 atatgaccag taccgcgtga tggaaaacga gaatggcaaa tggcgtttca cttcccctac
     4981 ccatacggtt ttggcttttc aacaagcgct gaaggaacta gaggcagaag gaggaatcga
     5041 aactcgatat tcacgttatc tcgccaataa tcatgtgcta cgacaaaaaa tgacagagat
     5101 ggggttccag cctttcattc aagaatatca ggggccattc atcacctctt ttctttaccc
     5161 taaggactgg cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa atggatttgt
     5221 gatctatccc gggaaaatca gcgatcatga atgttttcgt atcggaaata ttggcgagat
     5281 ctatccggaa gatattttgg aactgacagc aattatcaaa gactataagg agaacgttaa
     5341 tgattaaaac tgtaattttt gattgggcag gaacaactgt ggactttggc tgtatggctc
     5401 ctgtccacgc ttttagaaat gcttttctag aaaaaggaac ccaactgact gacaaagaaa
     5461 tccgctaacc gatgggaaaa ttaaaatggg atcatatcca acagcttctt tccctccctt
     5521 ctgtcaaaaa acaatggata gctatttatg gacaactacc tcaggaaaaa gaggtggagg
     5581 aattgtatca acaatttgaa cattatctgt ttaaagattt ggtcaagcat tctaccttga
     5641 aacccgacac attggaaacg atcgaacggt tgagaaacgc tgggatcaat attggttcca
     5701 cgaatggatt taatgctcct atgatgacaa tcgtagcaga aaccgcaaaa aaagaaaggt
     5761 attcaccaga cttcatggca acttttgaag acgtcgaagg gtatggtcga ccgtttcctt
     5821 atatgatcca taaaaacatg cagtacttca aaaacaagtc ggttgatgaa gtaatcaaag
     5881 tcggagatac ggtctcagat atccaggaag ggaaaaatgc cggagtgttg acagtaggtg
     5941 taattgaagg tagttcgcta atggggattt cttatgatga gtaccaaaaa ttagagatgg
     6001 ggaaaaagat cgtttaaaac agtctgttgc tcttagattc caacaagccg gcgctgatat
     6061 ttgtgtaaac aatttaaaag agttaacaga atacattttg tacgctgaca agaatccaga
     6121 atgactttta tacaaaataa gaggttgtga aaaagctgtc ctgtatcaag taataaaaac
     6181 agccaagcaa aaatagctcc tcatattttt cgtttggtta ggcttattac cgcagatgca
     6241 agcttttgaa caccgtttat tcgaaaaaaa aaagggagct gtgacacgac ttttgtcaca
     6301 gtccccttat ttgcaaacaa acagtgttta agagacaacc tctccactaa acggtgttca
     6361 ttcagctgac tctcggtatt tgctcaaaaa acgataaaat ttgaaaagct attttctctt
     6421 ttgtttctct aatactcgga tcagaacgct gatttcacat tctctttttc ttacatctta
     6481 tctggtgcat gaagtcctag caaacgaagg tcttctttca ataatacagt cactgcataa
     6541 actagagcta agcgtgcttc tttttgttca tcatcagcta aaatcttcgt atgagcatag
     6601 tatttgttga atgcttgtgc taatttgatt gcatgtttcg caatcactga cggttcgtat
     6661 ttttccccag cagacaagac cgtttcaggg tatttttgaa ctaatttaac cacttcccag
     6721 ctggtatcgt cattcaatgc atagtctgct gtttctgaag ggacaaatcc tgctttttcc
     6781 aacaggctga ctgcacgagc atgtgtatat tgcacataag gtccagtttc tccttcaaat
     6841 cgaacaactt cttccaggtt aaagtcaaat gtattcagtc gatcgttttt caaatcgtgg
     6901 aaaatgactg ccccaacccc aacttgttta gctacagcat ctttgttttc caaatcaggg
     6961 tttttctcgc tgatttgttc tttagcagat tggattgctt catttaatac ttcttctaat
     7021 aagacgatct ttcctttacg agttgataat tttttaccac cttgcgtgat caaaccaaat
     7081 ggaatatggt gcatgtcgtc agaccagtca aagcccattt ccttcaagac agctttcaac
     7141 tgtttaaaat gatagctttg ttcattccct actacatata gagattgttt gaagtcgtaa
     7201 gtacgtttac ggtacaatgc agcagctaag tcacgtgtaa tgtatagtgt cgcaccgtct
     7261 gacttcttaa tcaacgctgg attcaggtca taagcagata ggtcaacgat ctctgcacct
     7321 ttatcctcgt tcagcaaatg tttttcttct aaaagtttga cgatttcatc catcttgtca
     7381 ttgtagaaag cttcaccgtt caacgaatcg aaacgtactt ccagcagatc atagatttta
     7441 ttgaattctt tcatcgattc atcacggaac cattgccata attgaatagc ctctttatcg
     7501 ccttcttcca gacgtttgaa ccaagcacga gcttcttcat ttaattctgg ttctgtttca
     7561 gctacttcat ggaattggac gtataaacga agcaattcat tgattggttc tgctttaaca
     7621 gcttcttctg taccccattt tttgtaagcg acgatcagtt ttccaaactg tgttccccag
     7681 tcacctaaat ggttgatgcg gattggctga taaccgattt tttccataat gaaaccgatc
     7741 gaattaccga taactgtcga acgcaaatgt cccattgaga ttggtttggc aatattagga
     7801 gaagacatgt cgattggtac tgttccttga ttcccgatgt tgctatcccc ataatgttct
     7861 ttttctttca ctactgtttg caaaactttt ttgctgataa gttctttatt catgaaaaag
     7921 ttcaagtaag gtccgacaac ttcgattttt tcaaagttgg cagaatcgat tttttctgct
     7981 aaatctgcag cgatttgctg tggcgctttt cggtaaactt ttgctaatga aaaggctgga
     8041 aatgcaacat ctccgtgttc agctgatttt ggattttcta gtaattgctc gacttgttcc
     8101 agtgttaaat catctttgac agcgtcatag acagcttttg cgacaagatc tttattattc
     8161 atctttattc ctcctaaagt gttcccttga ttgttaaaca aaaaaaatct ctagcgacgg
     8221 catacttagt atgccatcac tagagacgag atttcccgcg gtaccactcc aattgtccta
     8281 aaaaaggacc agctccatct gatatcggta gacgaccgtc tgtttaaaac agaattctcc
     8341 aagtgcgctt cgttgtatct tcttcattcg gcttccacca cttccgaatt cgctagccaa
     8401 gaatgataca agtactctct tgttcaacaa atcttagtct tattaaactt tcttgaatta
     8461 tatcagaaaa aaaaacgaaa ggctagtcgt ttcataaaaa aaaaatccag atggcctaaa
     8521 ggagaagaaa taccacctgg atagctataa atcatttcgt aaatacgaaa gacttaacaa
     8581 attaatcagc tacgataatc gttccttctt cagaagctaa aagattctcg atattttcta
     8641 aagaagtgat gattgcctta gcgtttggtt ttgcttcaac aaatgcaata gctgcttcta
     8701 ctttcggaag catgcttcct ggagcgaatt gcttttcatc aatgtattgt ttcatttctg
     8761 aaacagtaac agtttctaat ttcttttgat ctggtttttg gtaattgaca tacacattgt
     8821 ctactcctgt caatacgatc agcaaatccg catcgatgat ttcggctaat ttttctgaag
     8881 caaaatcttt atcgattacg gcttctcgtc cttctaatcc agtagctgtt tctacaacag
     8941 gaatcccacc gccaccaaca gagactgtga taactccttg atcgaccagc gtttcgatga
     9001 cacgtgcttc tttaatcgag atgggtttag gggaagcgac tacttttctc catccacgtc
     9061 cagcatcttc tttgaatgtc acagtggagt cagcattcat ttgttctttg gcttcttctt
     9121 ccgtgtagaa tggaccgact ggtttacttg gatttttgaa tgaaggatca ttttcatcca
     9181 caataacttg agtgactaaa gaaaccacat ctttatcgat ccctttttcc ttcagtactt
     9241 cgcccatggc gttttgtaac cagtaaccga tagatccttc tgtcattgca acacatgtat
     9301 ctaaaggcat tgccggtgtt ttctcagaat cagcagcttg ctgctgaatc aataggttac
     9361 cgacttgcgg accatttccg tgggagatga tcaattcatc tccttgttcg ataaatttca
     9421 ctaaatactt tgctgtttcc attaacgctt cttgttgggc ttttgcactt gcatccgtag
     9481 acaagatggc attgccccct aaagcgacaa ctacttttcg tttggccatt tcattcgtcc
     9541 tttcttcagt tctaccgaat tttctagata acaaaacgag ccagcgtttt gtgattttcc
     9601 ctcaatgctg actcgttttt tcgtgagatg aatgtggtta tgcttaaacg cgagggataa
     9661 acaagttacc taatgttgct gccatgatcg ctttgattga gtgcatacga ttttctgctt
     9721 ggtcaaattg acgagcatat ttgctgcgga agacttcatc tgttacttcc atttcagtga
     9781 tgccaaagcg ttctttcatt tgttcaccgt aaacagtgtt tgtatcatgg aaagctggca
     9841 agcagtgcaa gaagatcaaa cgatctgtgt tgtgtgtttt ttctaccatt tccatattga
     9901 tttggtaagg tttcaacaat ttgatacgtt cttcgaattt atcttcttca cccattgata
     9961 cccatacatc agaataaagg acgtctgctc cgtctacacc tttatcaaca tcgtcggtaa
    10021 tcatcaattg tgcaccggat ttttcagcaa agccttctgc cattttgacg atttcttctt
    10081 caggttgtaa ttcttttggt gcaacgatac gcatattcgc tcctaaaatt gcacctgtga
    10141 ctaataaaga gttagccata ttgtttcgac catctccgca gtaagcaaca gtgatacctt
    10201 ctactgttcc aaagttttct tggatagtta aaaagtcagc gatcatttgt gttggatgcc
    10261 attcatcagt caagccattc caaactggaa caccagagaa ttctgctagt tcctctacca
    10321 ttttttgact gaatccacgg aattcgattc cgtcaaacat acgtcctagt actttagctg
    10381 tatcttctgt ggattctttt ttacctaatt ggatatcatt tgcacctaga tattcaggat
    10441 gtgcacctag atcgatcgct gcagtagtaa atgcagaacg agtacgtgta gatgtttttt
    10501 caaataaaag cgcaatattt tttccttcta gatagtgatg tgggatacca cgttttttca
    10561 agtctttcaa gtgttctgaa aaatcgatca aatattgtaa ttcttctttt gtaaagtctt
    10621 tttctgctaa taagcttctt ccttggaata ctgattcttt catattaaac attctccttt
    10681 agattgtttt tatttaagat taaagaggaa atcccgacgg cagctgacag taagactgcc
    10741 gtcaggaaac aaattacttc aagtcttcac ggacaagtgg ttggctcatg cagcgtggac
    10801 caccacgacc tcgtgacaat tcacttgaat tgatttctaa cactttgatg ccatagctac
    10861 gaagcaattc gttcgataca taatttcggt tataagtgac tacaacacct ggagcgattg
    10921 ccaatgtgtt tgatccatca ttccattgtt ctcgaggtgc aacgatctca tctccattac
    10981 cagttgggat cagtacaaga tcatctaatc caagagcagc tttcatcgtt tcgtgtaagt
    11041 tatctgaatg agtaatcttg atatcatcac catctggttc gattgtaaag acatccatct
    11101 taccgtcttt actttgaatt gctggatgga tagtgaattt atcgtgatct accattgtaa
    11161 atacggtatc caaatgcatc attgcacggt tgttcggaat tttgattgcc aacacttttt
    11221 caaatcctga attttttgcg aataggtggc gtgctaattt ttctaaagct ttcgcattag
    11281 ttcgttgaga aatcccaaca gctactactt tatcgcttaa gatcaattcg tctccgcctt
    11341 cgatatgatc tggattttca cgatccaacc atacttcgat tcctttattt gcaaatcttg
    11401 gatgatgttt caaaatatat tctgtaaaca tcgattcacg acgacgtgct tcaaaagtca
    11461 tcttattgac tgtcatccca tttcccattg atgcggaagg gtcgcgtgta aagtacaagt
    11521 ttggcattgg gtccatgtaa aatggatagt catcgtctgc tgacagatca tatagactgc
    11581 tagaacgtac ttcgatatct tttgtacgaa ccccagccat gattttatct accatttctt
    11641 gcgtttccat tgatagaagg aactcatgaa gaccctctct tacagcattc gatgcaatat
    11701 gagattcatc caacatttta ttcaagaatt gttctttgac attgcctgcg tcaatcgctt
    11761 ctgccgctaa tttttccaag taaagagtct cgacgccttc attttgcaat gtcttcgcaa
    11821 agttgtcatg ttcttcttga gcaatcggaa gatatggaat atcatcgaac aacagacgat
    11881 ccataatatc tggtgtcaaa ttttccactt cttgtccagg ccgtttcagt aaaactgtct
    11941 ttaatttccc tatttcagag aaaacgtgaa taggtttatc catgttgtgt tgcctccttc
    12001 tttttgatta catgtatagg atatcctcct tttgtaatca ctttcataat tttgtctcat
    12061 tcaaaatgag acattcctca cattaatatt cgataattaa ataattaaac gtttttctat
    12121 ttttaagc
//
LOCUS       DAJSKS010000082        12084 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJSKS010000082 DAJSKS010000000
VERSION     DAJSKS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.084"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            140..460
                     /locus_tag="ON396_001857"
     CDS             140..460
                     /locus_tag="ON396_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113937.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="ON396_001858"
     CDS             912..1112
                     /locus_tag="ON396_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3113938.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="ON396_001859"
     CDS             1259..4048
                     /locus_tag="ON396_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR3113939.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="ON396_001860"
     CDS             4095..4622
                     /locus_tag="ON396_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HCR3113940.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="ON396_001861"
     CDS             4643..5833
                     /locus_tag="ON396_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR3113941.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="ON396_001862"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="ON396_001862"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR3113942.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="ON396_001863"
     CDS             7966..8640
                     /locus_tag="ON396_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR3113943.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="ON396_001864"
     CDS             8637..8978
                     /locus_tag="ON396_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113944.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="ON396_001865"
     CDS             9008..9367
                     /locus_tag="ON396_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR3113945.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9891..11975
                     /gene="cadA"
                     /locus_tag="ON396_001866"
     CDS             9891..11975
                     /gene="cadA"
                     /locus_tag="ON396_001866"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCR3113946.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
BASE COUNT         4031 a         2110 c         2600 g         3343 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa acagtattaa
    10681 gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct tatgaaaatt
    10741 ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa gcaccagctg
    10801 aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga ttggccgttt
    10861 tattagctgt tttacctcca cttgttgttt ctagagcggc gttttctgaa tggatctatc
    10921 gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc gtgccactta
    10981 gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa ggttcaaatt
    11041 acttggagtt gcttgcacaa actgagacag tggtattcga taaaactggc acattaacga
    11101 aaggtgactt ttcgatccag acgatcgatc caacaggaat tgatccaaag aaatttatgc
    11161 aatatgttgc aagtgcagaa cagttttcta cacatccgat tgctcaatct gttttagaag
    11221 gctacgcagg tccgctttta ccaacagcta atatccagga atttgcaggt gaagggatct
    11281 tagctgaagt tgatggcaaa caagtattag taggtaatca caaattgatg gaaagatttg
    11341 aaatctcttt tccttcaagt caagaaatcg gtacattgct ttatctagcg atcgatcaaa
    11401 gttacagtgg ttatcttgtt attgcagata ccttgaaaga agatgccgta gacgcattgg
    11461 ttcaattaaa acaggctggt gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa
    11521 ttgctgatca tatcgggaag caagtaggcg tggacaagat atatagcgaa ttgcttccag
    11581 aagataaagt gcagcggtta gaggaaatct tacaaagtaa caataaaaag accgcatttg
    11641 tcggagatgg aatcaacgat gcaccggtgc tagcaagagc agatgtagga atcgcaatgg
    11701 gtggattggg aagcgatgct gcaattgaag cagcagatgt agtcatcatg aatgatcaac
    11761 catcgaagat tgcagaagca atccatttag caaagaaaac attgaaaatc gtcaaacaga
    11821 acattgtctt tgctattggg ataaaaattt tggtactttc cttaggtgct tttggttttg
    11881 catcaatggg agatgcagta tttgcagatg ttggggtgac agtattagct gttttgaatg
    11941 ccatgcgaag cttgcatgta aaaaataaac gttgatcaat gaagaggacg aatgaacgtc
    12001 ctcttttttt tatatccttt tcttaatgaa aaatgtaaag aaaatagata ggattataaa
    12061 ataactaatg tttttaattt tgca
//
LOCUS       DAJSKS010000083        12024 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJSKS010000083 DAJSKS010000000
VERSION     DAJSKS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.085"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            155..934
                     /locus_tag="ON396_001867"
     CDS             155..934
                     /locus_tag="ON396_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCR3113947.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            927..1244
                     /locus_tag="ON396_001868"
     CDS             927..1244
                     /locus_tag="ON396_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCR3113948.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            1488..3098
                     /locus_tag="ON396_001869"
     CDS             1488..3098
                     /locus_tag="ON396_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3113949.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            3255..4103
                     /locus_tag="ON396_001870"
     CDS             3255..4103
                     /locus_tag="ON396_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR3113950.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            4334..4813
                     /locus_tag="ON396_001871"
     CDS             4334..4813
                     /locus_tag="ON396_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113951.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            4846..7074
                     /locus_tag="ON396_001872"
     CDS             4846..7074
                     /locus_tag="ON396_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3113952.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            7203..8846
                     /locus_tag="ON396_001873"
     CDS             7203..8846
                     /locus_tag="ON396_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR3113953.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(8919..9464)
                     /locus_tag="ON396_001874"
     CDS             complement(8919..9464)
                     /locus_tag="ON396_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCR3113954.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(9487..9972)
                     /locus_tag="ON396_001875"
     CDS             complement(9487..9972)
                     /locus_tag="ON396_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR3113955.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(9962..10774)
                     /locus_tag="ON396_001876"
     CDS             complement(9962..10774)
                     /locus_tag="ON396_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HCR3113956.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(10913..>12024)
                     /locus_tag="ON396_001877"
     CDS             complement(10913..>12024)
                     /locus_tag="ON396_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HCR3113957.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         3887 a         2224 c         2397 g         3516 t
ORIGIN      
        1 cccaaattat tctcgtaaat aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat
       61 ataatcttgt ttttaaataa aacggctaca atttagcgaa tttatgttac aatgagtccg
      121 ctgtttattt gttgtaatta aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg
      181 gcttcaagct ttgcatgcag tgatgccact ttgtataagc tatataccag taggattagc
      241 ttgcggagta ttgttgcaaa aagttggatt tgatcctctc ttatcaggac tgttatcgat
      301 tctggtcttt tccggtggcg cgcagttttt agttgcatct atgcttacga cgcaagcatc
      361 atttgcaacg acattattga tggttttctt tttagaatta cgctatacat tattaggagc
      421 aagtcttgcc ggttttatga aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc
      481 actgaatgat gaaaactatg cggtgaacta cttaaagttt tcaactgacc cttcctggaa
      541 taaaagaaag gctttatatg tcaattggtt ctcaatgact gcatgggccg gtagcaatat
      601 gatcggtaat tttttcggct cggtgattcg agtagatgct gaccttgtcc attttgcttt
      661 gacggccatg ttcattttca tgtttatcat gcagatgaaa agcgcactac tgatctttac
      721 aggtttattc tcaggcgtat taggggtcgt attcatggta ttattccaaa atactttcgg
      781 attgattgca gcaacagtca ttgcttcatt tgccggattt atgctggaga aggcattcaa
      841 aaaagaaaaa gctaagaaac taagagaccg cggtaaagat gtcaatgagc cgctcttcaa
      901 cttcccggaa caggaggcca atcaccatga ctaaactata tttgctactt ttagttgctt
      961 gtctgttcat cgtggcatat atcccgcgct tgttcccgat gctttatttt actcatcgaa
     1021 aagtgccatc ctggttcagt gactggatga aatatgtacc ggtggcatta tttgcggcgt
     1081 tagcttttaa agatgttttc attactcatg aacatctgga tattgcttgg aatatcaaaa
     1141 tagctgcaat gattctagta gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag
     1201 ttttgacagg acttgcgtct gtctttttgc tgtctatgct ttaaactgtt ttattttatt
     1261 aaaaacagag attgcgacat aagttgttca aactgatcta tccgaactgc atttgcaaca
     1321 gtcaattctg gatgcaagtt tattcgtagg gataaaaaag attagtgttg gcttatgatg
     1381 caagctcttt tttattttag gaattagaga gggatttgtc cttctatgat tttttgctat
     1441 aatagggaag atgtttattt tatgaaagaa tagttgaggg aatttaaatg aataaaaaat
     1501 tgatgcaagg aacattttgg ctaacctttg ctaatcttct atgtaaagtt ttaggggtag
     1561 tctatttgat tccctggcta agtatgatgg ggaacaatca agatggtatg cttgctacga
     1621 cattgtataa tgttggctat cttccttatg gattgttcct gatgcttggt acagtcggat
     1681 ttccaaatgc gattgcaaaa aaagtagctg tagccacaaa agaaggagat aacgctgctt
     1741 gtcggctgat ttttagaagt acgatcaata tcatgtttgt catcggtatc gcctctgctg
     1801 cactgatgta tttatttgct cctttactag ccggaatcag tccgattgct aacgttaata
     1861 atgggatact agctatcaga agtttatgcc catctttgat tgctatccca attcttagtg
     1921 caatgagagg atatttccaa ggaaaaaatg atctgcggcc ttatggaact tctttgatta
     1981 tcgaacaggc tgtgcgagtg atcgttatct tggctggtac ttattacttg cgcgtattga
     2041 ctgacggaac catattagaa gctgtattga tcagtacagt cgcttctttc ttcggtgggt
     2101 tagctgcgat tatccatatg tatatcgtcg gacgcagaaa agacttcttc gaacttaaag
     2161 attttttgat ttccagccgt tatttcgaaa gagaaaaccg atctgcctct gtctctatca
     2221 ttcgggaaac actaccgttt atttttgtcg gctcagtcat tactatcgtt caaatgatcg
     2281 accaagtaac gatgaaacca ctgcttcact ttttccgtcc agagatcgca gatcagcaac
     2341 tggaattctt attcagccgt gcttctgtca atccaaataa attgacattg atcctgatct
     2401 ccatggtagg aacagtagct ataagcagct tgccaatatt aagcacgatg aagaaaaaag
     2461 atcggataca aatcgaaaaa acagtaggag atagtttttc gattgcgtta ttgattcttc
     2521 ttccttcatt gactggaatg tctttattag ctggaccact atacacattg ttttttggct
     2581 atgatcctga aagtgtcgga tatttccaaa tggctttgct agcttctttg tttttctctt
     2641 tattcacgat cctatctacg atgcttcaat ctttgaatca ccatcgtttt gcgatccgat
     2701 taactgtaga agcaattatc ctgaaagtca tcttccaagt aatcggatta ggattagttg
     2761 gcggatacgg gatgagctta tcaaatggct tagcattcgg tattgtcttt gttcgcggct
     2821 atcactttat gtgcaaagaa taccgaatag cacctttagc aaagatcagc aattttttct
     2881 taaaaacttt ccgctccacc ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc
     2941 tgaatcagcg attgtcaatg gaatcaaaaa gctatgcaat gatttattgt gtggctgtag
     3001 gaagtattgg cggcttagta tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt
     3061 tgaaaaatgt taaacatcat agcagaagca aagaataggt taactaatcg ttttttgctg
     3121 tcacttgaaa aacggcaact cgtatgatta cgcttgtcgt ttttctattt tccaatttta
     3181 gtttcatatc taatcttact tatgatatag ttataaggtc tggaaaaatt ctttgaaacg
     3241 aggaaattaa acacatgaaa cttgcagtag ttacagatag ttcggcatat ctgccggaac
     3301 gtatcaagaa ccatcccgac ttattcgtta ttccaatccc tttgattatg gatgggaaaa
     3361 tttataacga aggaatcgat atcgaagcgg atgaatatta tggactttta aaaaacagta
     3421 aagattttcc tactacttca caacctgtcg taggggaagt tttgggttta tatgaggaat
     3481 tgaaagagaa agggtatgac acgattttaa gtatccatct ttcttcaggg atttctggct
     3541 ttgtcaacac attatttgcg atgaaagatg acatcagcgg agtaacggtc attccttatg
     3601 actctaagat cactagtatg ccgatgggac acatggtaga agctgcctta gacttaaatg
     3661 atgaaggtca gtcccttgat gaaatacttg cacatatcga tcgaatccga gataatacct
     3721 acgcttatct gattgtagat gatttgaaca atttagtacg aggaggacgt ttgacaaatg
     3781 gtgcagcgct gattggcagc ttattaaaaa tcaaaccgat cctgacattt tctgaaggga
     3841 aaattgtttt attcgaaaaa atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa
     3901 ttattggtag aagaaataac gaaatacaac cgccagtcaa actttacgtg atacatgcaa
     3961 ataatttaga agtagcgatt gcggagaaag agaaattgca gaaaaaatat ccaaatgcag
     4021 aaatcgaaat tggtcatttc ggaccagtca ttggtacaca tttaggtgaa aaagccatcg
     4081 gcattgccat ttcagcgcaa taattccttt tattcaaaaa acataccaaa aatattccaa
     4141 catcatcttg gactttctac atgttactaa tgagcgtgta aaagaacgag atggtgtttt
     4201 tatttctatc aatgaagctc tttttctttg caatcctcac ttcaatcctc tataatgtcg
     4261 catatagttt agtaaatgag taaactacta atttacttat ttactaaaaa taaaaggatg
     4321 aggagttaaa ataatggaaa taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga
     4381 agactatgat aaagttgatg gaagaaatgc attaaatacg gatgcaaaag ggctttcact
     4441 gggccttgct cagtggaatg atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca
     4501 tactggagaa aaatggtcac gacagtcgga agaattacca atcacacgta ttttagattt
     4561 agcgatctta gctgcacaag gaagtttata cttccaagat gcttatcgtt atgaaaaatt
     4621 ttatgatcca gaaaatccag tcgtagacat cattggatta caagggaacc gaatgacggt
     4681 agaagtagat accaaaaatc caaagatcga tgaagacatc attttgtatt acgatacact
     4741 acaaaaagac ggagaactga ttggggaacg attccgtatt ttaaaacgtc ttttagatga
     4801 gctaggatat taatagacag agatgatcaa aaggggaaga gaagcatgga gaatttctca
     4861 ctaacagaaa accgaacctt ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa
     4921 agccatcgga caagtgcaga agaatggcgg atcgcctccg aaatcaaagg gaattggcaa
     4981 gatcctgaaa tgaaaatcag taacgtactt ttggaaggag atgccgggtc ggggaagaca
     5041 caattagcca aagcactatc tgctgatctg caacttccct atacaaaagt gacctgtttt
     5101 gcagatatgg acaaatcaga tgttttcgga gccttattac ctgtagtaga ttctaataag
     5161 gaacaagacc aagaattact agaagctatt taccaaacag atacacttga agctgtactg
     5221 acactgatcc agaaccatta cggcattgat cctttttctg ctaaagaaaa attagcagag
     5281 ctagttcaaa ggatcgaagc tgattatcca gatgcaatcc attacagcta ttacccttct
     5341 gaaatcgttc gagcgattga aaaaggatat ttattagaga ttcaagagcc gactgtcatc
     5401 cgagatgcct ctgttcttgt ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat
     5461 ttaccgacag gaatcattcg tcgccatcca gattgcgtca ttgtgatcac tacaaatcga
     5521 aattaccaag gaaatcgccc attaaatgaa tctttaagag atcggatgca acatgcggaa
     5581 aaaatggatc ttcctgcctt agaagtcatg gcagaacgtg cgatggcgaa aactatgatc
     5641 caaaaacaag aacttctctt aaagatggca gagattatcc gtttgttgga tgaaactgca
     5701 aaagctaacg ccatcaaagg tgtagcagga atgcgttctt atttttattg ggcaaatacc
     5761 tataagcaag gacaagattt actccagtcg atttatccaa aagttttgta taaattatcc
     5821 actgattcag atgaattggc aattcttgaa caagctttgt ctgacagtgg attgctactg
     5881 gaacttcagg acatattgag aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa
     5941 ggaagaacca ttagcgccga agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa
     6001 attctgcgct tagatgaaaa gaaatcgttg tctgctgaag aaccaaatga gggagaagag
     6061 aaacaagaag aaacaataga aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt
     6121 agcgatatcg aaggaaaaaa tcaaaaagaa caacaaacag gagatgattc tcaagaaatg
     6181 tctgcttctg acatggaagc acatgataag gaaatcaaga agcaactgaa taaagaagcg
     6241 cgacaaatca tgaaggaaac gattcatgaa aaagaaggtc tcatcgttca ccgaccagct
     6301 ttccaaggaa aaacgccgga agcacgtgag ctacacatgc aagtaatgcc aatagtagaa
     6361 tcacttagcc ggcaaatttt agagttactg gataatgaac aaacagagac gtaccaaaaa
     6421 ggaaaatacg aaggtcagcg gttcaatgca agcaaagtag catatggtga tttgagaaat
     6481 tttgacaaaa agaacccgcc acatgagcaa ccttctttag cggtagctgt acggatcgat
     6541 gaatctgggt caatgatacg ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc
     6601 attgccgaat tttctaagaa agtcgatatc ccattgttga tttacggaga tacagcagac
     6661 cgttcgtcaa gagaaaagac ttctttgttt tcttataaag aatttgaaga cggatttcaa
     6721 tggttaaatg aaaaattagt cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca
     6781 cttcgtttag cagcagaaaa attaaacaga caatccgcaa caaccaaact gttgcttaat
     6841 attagtgacg gtcagccaaa agcgttgccg gattatacag gcaaaaaagc aaaagaagat
     6901 atccagtctg ttttgaaaga atatgaacgt caaggaatcc tatttgtctc cgcagctatt
     6961 ggccaagaca aagaagaaat caagtcgatc tacggtgaaa gtcgttttgt agatattact
     7021 gacttgaatg aatttcccaa gcaaatgatt caactgattg cccgctatct atgataaaat
     7081 aaagctcaac gaacgcactt agcagattcg ttgagctttt cgtttctagt taccggtaac
     7141 aaggatactt ctaaaataga atcggttata atcaagaggg accgttaaag gagtgaaacg
     7201 atatgataca agaacaaaaa tggtggaaag aagcagtagg ctatcaaatc tatcctgcta
     7261 gtttcaaaga tagcaatggc gatggtcaag gagacatcaa tgggatccgt gaaaaactta
     7321 attatttgaa agatcttggt atcggcttta tctggatcaa tccgatctat caatctccat
     7381 ttgtcgataa tggttatgac gtaagtgact atcaagatat agaagagaga ttcggaacga
     7441 tggaagattt tgatttattg ctaaaagaag cacatgaatt agggatcaaa gtgattatgg
     7501 atcttgtcat caaccatagt tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta
     7561 aagaaagccc gtatcgcgat tattacattt gggtcgacgg ggtagatggc aaagaaccga
     7621 ataattggac atcgattttc ggcggttctg cttgggagta ttctcatgaa actggacagt
     7681 attatctgca tgtttttgca aaagaacaac cagacttaaa ttgggaaagc gagaaattga
     7741 aagaagaact attcaatatg atccgatggt ggttggataa agggatagat ggatttcgtt
     7801 tagatgcgat ttctcatgta aaaaaagatg aatattcagt aaaagccaca gagaaccctt
     7861 tctcaccctt tcaaaatgtc tcaggaatcg aagaacattt gaccgatttg aaacatgttt
     7921 ttgaagagta cgatatcatg actgtcggag aagcaagtgg tgtcactgca gaagaaggac
     7981 cacaatgggt agggaaagac ggttatttcg atatgatatt cgaatttgat catatccata
     8041 tctggcagca agaaaaagaa gggcaattag atgtccttaa attgaaactt gcattaagcg
     8101 cttggcaaac ttcattggat ggtatcggat ggaacgcttt atatatggaa aaccatgatg
     8161 ttcctcgtgc cgtttcagtg tttggagata ctcgtcccga tttttgggct atgtctgcaa
     8221 aagcaatcgc tatgatgtat ttctttttgc aaggtacgcc atttatttat caaggacagg
     8281 aaatcggaat gacgaatatg ccctttgaat cgatcgatca agttgatgca gtagattcga
     8341 aaagacttta taaaaggctg cttgcggaag gtaagacaag agaagaagca ttggatatca
     8401 ttcgtgaaac aacgagagac aacagcagga cacccatgca atggacttcg gaacaatatg
     8461 ctggtttttc tacacatgaa ccttggctga tcactaatcc aaataccaaa acgatcaatg
     8521 tagaacaaca agaatatgaa ccagagtcgg tcctgcaatt ctataagaac atgatccgca
     8581 ttagacaaac caataaaggt ctgatatacg gttcttataa agaatatctt catgaacatc
     8641 cacaacttta tgtctatgaa agatatttag aagatgaaca atatcttatt atggtaaact
     8701 taacagaatc acttgctgac tatgaacttc caaaagaagc agatcagtcc tggacattac
     8761 tcttgtcaaa ttcctctagc ggagaatttg aagctaaagg catactagca ccttatgaag
     8821 ctcggttata taaaacaaac aagtaaaact tcctcgttaa agttcattag ttggactttg
     8881 cgaggaagtt tttcagattg ctctgtcaat tactcatttc aataattcgc tctaggacca
     8941 ccgatatatt taggacgaga gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa
     9001 cgaggtctga caggtacttg gacatgtttg atatgcatac cgattgatgt gtcaccgata
     9061 tcgataccgg cttgtccaac tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa
     9121 gcagcaacag aacaagcacc tccagcatgt agtgcaggaa caacagagac aatttcgaaa
     9181 ttttttgctt ctgccactgt tcgttctact actaatgctc ggttcaaatg ttcacaacct
     9241 tggacagcta gcgcaatttc tttaggatcc aacaactctt ttaacgtacg gatgatccat
     9301 tgaccaactt ccgcactgga attttttcca atatgtcctc caacgacttc gcttgtgctg
     9361 catcctaaaa caaaaagatc accttttttc aagtgtgcct cagccaatat gtcattaact
     9421 atttcagtca attggtcttt taattctttt tctgacaact tcattttcta aaaccttctt
     9481 tttcaattaa tattgcttcc gtgcttgaat acgttgaaga cttgcagtta taggaaaggc
     9541 aagtcccatt ccaatcagat tttggaagat gtttcctggt atagaagcca ctccagccgg
     9601 ccaatcatat aaaatccaac cggcgatacc atagccgatg atcataacta agcttcctaa
     9661 taaaagccct aatccccaca tacgagtaga agatttgctg aaagaaccag caacatatcc
     9721 ttgtaacccg tggatcacta atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc
     9781 gatcaataga ccactggaag caccaacaac taatccaccc gttggaccat aaagaaaagc
     9841 agcggtatag atcccggcct cgcataatgt cacaaatcca ttcgtagccg gtacagggat
     9901 caaaattgtt aatgaaaggg caactgtcaa cgcaatcagc atagcataga tcgttaattg
     9961 cttagttttc atttctcatt cctccttttt gaataccgcc agaccagaca cttccggtat
    10021 gatcgttcat cagcacgcca ttagaaatac aatgggcaac aaatttttta ctttctgcta
    10081 ctgctttggt aaaatctgcc cctaacacaa gattcgctgt tattgcagaa gaaaaggcac
    10141 aaccggcccc gtttactgtt gtagtgtcga tttttttatg agaaaatacc tcataatgat
    10201 caattgaata gagaagatcc acagcttcat ctccagccat ccttgtgccg cctttgatca
    10261 caatgttttt tgttcctgaa gcagtgagtt ctttagcgag atggatcatt tctgttaacg
    10321 aagagggaag agcttgacca gaaagtaaag cagcttctgc caaattaggc gtaatcatcg
    10381 taacaagtgg aaaaagttct tcgatgagct gcgaaatata caattgattg atcacagctt
    10441 cagtttcttt gaatgccaaa acagggtcta aaatgattgg gatattttgt tttgttttta
    10501 tgaagtctcg aacaaccatg attgcttcac ttgaattcaa taacccaatt ttgataccat
    10561 ctaatgaagt cattttatca atcgaaacta actgtcgttt aagcaattcc aaagatatag
    10621 attccagcat aaatcgctca ttaatatcta gtgcccctat agaagtcaag gcgcttagtc
    10681 cgaatagacc aaaattctca aaagttttta aatctgtttg gattcctcca ccagcaaatg
    10741 gatcagaacc gccaattgtc aagattcttt tcataacatt ttcctccatg aataagagta
    10801 taacgaaatt ttcaaaaaag aaaaacagcc aattggcata ttttcatctc atccaattta
    10861 aaaaaacaaa aagaaaagaa agaccaaaat ggatctttct ttccttaaat agttaggact
    10921 tttgtgattt taattctttc tcgatttgaa gccgttgcac tttcattgta gctgttcgcg
    10981 ggattgcatc aaaatcacgg atgataggga cgttcaagcg aggaagttca tggacttgct
    11041 cccaccatcg attccaatcc atttctttat caggagctaa agcaataatc ggttgtggag
    11101 aatgattttt atcccttaca atcactactt ctgccaaaaa atctaacgaa tctaataaaa
    11161 agtcttcaat agctaatgta ctattgatcg tttcgatcaa atccacttgc cgatctttca
    11221 aaaataggtg gccatgctta tccataaaac cgtaatctcc gctatcccac caatcgccat
    11281 aaacattttc ctggaaccgt gcatcttcct tgtaataagt caaagcccgt cctttagaca
    11341 gtaattgtat atgaccatct gtgttttcag gtaggacatt cccaactgaa tctgtaatcc
    11401 gagcgcttgt catgtcttcc agtccaacgc ccatatctct tgcatcactt gttttcaaac
    11461 tctctagtgt atgagcctta agaatcattg gtccgcattc actttggcca tatacttgaa
    11521 gaaataccgc atcctgtgcc atcgacgttc gcaaaaatgt cttcattgtc tggttgttta
    11581 tagcatcaaa agtggagtga tagtatcgga tacccgaaaa agcttctgga tgctccttag
    11641 cagtaaaacg ccactgaaca aagttattcg gatgggtctc tagtgcaatt ggccggtgag
    11701 cttccaacat atgaacaact tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc
    11761 ccatggacat caaagaagag ataccaatat tgaatctgga atgcactgga gagatgtgaa
    11821 aagcgattaa tttcttttcc gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc
    11881 atcccataga atgagcagaa tgacaaataa gtttagggat accagttgta ccagaagtat
    11941 gggtcatata agaaatttca tttttgtcta aaaatgattg agaaacagac aaagcaggtt
    12001 gttccattaa ataaagaata ggaa
//
LOCUS       DAJSKS010000084        11676 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJSKS010000084 DAJSKS010000000
VERSION     DAJSKS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.086"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(227..712)
                     /locus_tag="ON396_001878"
     CDS             complement(227..712)
                     /locus_tag="ON396_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HCR3113958.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    802..1147
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1192..1833
                     /locus_tag="ON396_001879"
     CDS             1192..1833
                     /locus_tag="ON396_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3113959.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1852..2487
                     /locus_tag="ON396_001880"
     CDS             1852..2487
                     /locus_tag="ON396_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3113960.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2505..3320
                     /locus_tag="ON396_001881"
     CDS             2505..3320
                     /locus_tag="ON396_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR3113961.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3639..5075
                     /locus_tag="ON396_001882"
     CDS             3639..5075
                     /locus_tag="ON396_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3113962.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5264..7711
                     /locus_tag="ON396_001883"
     CDS             5264..7711
                     /locus_tag="ON396_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR3113963.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7941..9008
                     /gene="rlmN"
                     /locus_tag="ON396_001884"
     CDS             7941..9008
                     /gene="rlmN"
                     /locus_tag="ON396_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCR3113964.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9201..10304)
                     /locus_tag="ON396_001885"
     CDS             complement(9201..10304)
                     /locus_tag="ON396_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR3113965.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10857..11405
                     /gene="aac(6')"
                     /locus_tag="ON396_001886"
     CDS             10857..11405
                     /gene="aac(6')"
                     /locus_tag="ON396_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCR3113966.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3877 a         1968 c         2516 g         3315 t
ORIGIN      
        1 taaaattccg atttataatc gaaaaaagaa gcatttttat atatgcttct tttttcgtag
       61 aatcaataaa tatggatttg gctaactatg gaagaatact cgtattagtt ttcagcgttc
      121 atactagttg ttacattgtt tgattggcca aaagaatttc tgatatattt tgtgctaaat
      181 aaagaaaaag acgcttgaat tgggaaataa gaaaactact ctatcattac aaattttcat
      241 aaactggcag atgttctaag taaacaagta tcttttcaaa gtcggaaaaa tccataatca
      301 acgtagtggt attgatattt ggatgaaagc ctacggtatc ttgacgatca atagcagcat
      361 cgatcacgac ttggattttt ttctctttgt cgtgcatcaa tgctaatgga gtcactgtac
      421 ctgctggaac gcctaggagg tcaggaagct gttcttcaga aacaaaagat aagcgctttt
      481 cttttaattg ttcagctagt tgttttaaat cggcgtgctt ttcatcaggc aagatgacaa
      541 gatagatttg ttttcctttt cttgatttca ataatagatt ttttacttgc ggaccaggta
      601 gggaaaaagg gacatttttg actgacgtaa tcgcaggatg atcgactttt tgaaaagaga
      661 tacccagttt ttttagtagt tcataagctt cttgttcggt tgcacgactc atcgcgcatc
      721 accctccttt ttttattagt gtatcataaa aaagagttga caaatgaaaa ataatcatgt
      781 aaattacacg taaagttaaa acaaatgaca tcgaaaagaa gagtagccgt taggtgtttt
      841 tagagagtct ctggttggtg gaaagaggca gcagttaacc gtgaaacaca tcttggagtc
      901 gattttctga agcgtatagt aggaaaatcc ggggagcccg ttacagctgc ggtcgttgct
      961 agatgaccag ataaggctgg cttttccgtg aggagtcggt aaaaaaaggt ggaaccacgt
     1021 gattaacgtc ctttgatctt cggatcaaag gactttttgt tttcatctga acggcaacga
     1081 ctgtgtgagg ttttttctgc aaggaaaaaa taaacagagg tggaaccacg tataatacgt
     1141 cctcttggca tttgccaaga ggattttttt attttaaagg aggaaaagaa gatggagtat
     1201 ttgattgaag ttatgccgca attattagta ggagcactca caacattgaa attattttta
     1261 tttaccttga ttggctcatt accattaggg attgttttag cattaggatt gtctttgcat
     1321 tttaagccgc tacgctactt gcttcaaaca tatgtatggg ttatgcgggg aacaccgctg
     1381 ttgttgcaac tgatttttat tttttatggg ttgccgacga ttgggatcgt ttttgatcgt
     1441 ttcgaagcag cactcattgc ctttatctta aactatgcag cttattttgc agagatcttt
     1501 cgtggaggga tacaatcgat tcctcaaggt caatatgaag cagcgcaagt tttgcaatta
     1561 agtccctttc aaacggtgac gaaaatcgtc atcccacaag tgacaaagat tgttttacct
     1621 tctgtaggga atgaagttat caacttggtc aaagattctt ccttgatcta tatattaggt
     1681 ttaggtgacg taatgcgtgc tgggaaaatc gcgatggaac gcgacgcgac tttactgcct
     1741 ttgctaggcg tggcaatcat ctatcttaca ttgactggta ttctaacaat cataatgaaa
     1801 caagttgaag gaaaattaaa ttattaccgc taactgatag gaggaagaaa tatgattgaa
     1861 ttgaagaaag tgaacaaagc atttggtgaa aaaaaaataa tcaaagattt gaatctaatc
     1921 attccagatg ggaaaacatt agctatcgta ggtccttccg gtggcggtaa aaccacgctt
     1981 ttacgtatct tggctggctt agaaagttct gacagcggag aattttattt agacggtgta
     2041 ccgttcaacc cattcgagct taaaatgaaa gaacaagttg taggagtcgt attccaagac
     2101 ttccagctat ttccacatct gaatattttt gaaaacatta ccttagcacc aaaaatggta
     2161 ttgaaacaga caaaagaaga atacaccaaa aaagcagaag aattagcggc ttcattaggg
     2221 attagtgatt tattgaatat ctatccttat cagctttctg gcggacaaaa acagcgggta
     2281 gcgattgcca gagcattggc tatgaatcca cgggtattgg cttatgatga accaacaagt
     2341 gctttagatc ctgaattacg ccagcaagtc gagcgtttga tcctatcatt gaaagaagat
     2401 ggggtaaccc aaattattgt cacccatgat ttagtatttg cagaaaatgt cggtgatcaa
     2461 ttactaaaag tttccccaat tcattaagaa aggagaagat agagatgaaa aaaaccagtt
     2521 ttctattcgc tttgatcgca ggtcttcttc tttttgcagg ctgttcgaac aaaaaaactt
     2581 cagcagataa ttgggcgact atcaacgaag agaaaaaaat cgttataggt ttagacgata
     2641 catttgttcc tatgggattt agagataaag acgggaagct cacaggattt gatattgatt
     2701 tagccaaagc agtctttgaa caatatggga ttacggtaga ctttcagccc attgactggt
     2761 caatgaagga atttgaactg aacaatggaa cgatcgattt gatatggaac ggctattcca
     2821 aaacgccggc aagagaaaag aaagtgcaat ttacaaaacc ttatatggaa aacgatcaag
     2881 tgctgatcac acctaaagaa tcaggaatca caaactttgg acaaatgaat aataagagat
     2941 taggtgcgca aaatgggtca tctggttatg atgtatttac gaaacaacca gaagtattaa
     3001 aagagatagt caaaaaccaa gatgctgttt tgtatgatag ttttaatgaa gcattgatcg
     3061 atctgaaaag cagacgggtc gatggtctgc tgatggataa agtatacgca gactattatt
     3121 tgaaacaaag aaacgaacta gaagacttca acataacaaa aggttcttat gagagtgagg
     3181 attttgcagt tggtgcacgc aagtcagata gtacattagt gaaaaaaatc aatgaagccc
     3241 tagatcaact agagaaaaca ggtgaattcc aaaaaatatc cgataaatgg tttggcgaag
     3301 atgttacgcc gagaaattaa caaatagggg actgtgacaa aagacgtgtc acagtccctt
     3361 tttccgaata aacagtgttc tcctgccaac tccttgtgac tcggagctga atggcaggct
     3421 cacaacctct tggtaaacgg tgttcaaaag ccagtttttc ggtattaagc caaatttatg
     3481 caaaaatgta gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcgacttt
     3541 ttcacaacct cctttacata attttatttt gttgtggaag gggtttcgtg ttattctaaa
     3601 agaggaacga ttgaaggtta taggaaagga agagatgaat ggaacacaaa gaacttacct
     3661 catttccaaa agattttctg tggggctctg cttctgcagc gtatcaagta gaaggagctt
     3721 ggcaagaaga tgggaaagga gaatcagtct gggatcgatt tgtacggata cctggaaaga
     3781 catttaaagg aacgaacggt gatttggcag tagaccacta tcatcgttat aaagaagata
     3841 ttgctttaat gaaagaacaa ggtctgaaag cttaccgctt ctctgtagct tggacacgga
     3901 tttttcctaa tggacgagga gaagtcaatc aagctgggct cgctttttat gagagattga
     3961 tcgacgaatt gattgaaaac gatattgaac ctatgttgac tttatatcat tgggatcttc
     4021 cacaagcatt gcaagatgaa tataatggtt gggaatcacg tcagatcatt gaagatttca
     4081 ctcattatgc agaaacatta tttgaggcat tccgcgggaa agtccattac tggattagtt
     4141 tgaatgaaca aaatatcttc acttcattag gttatttgtt agcagcacat ccgccaggtg
     4201 tgacagaccc gaaacgaatg tatgaagtta accatattgc taatttagca aatgcatctg
     4261 tcatcaataa atttcacgag ttagaaatgc ctggaaaaat cggaccaagc tttgcgtata
     4321 cgccaaatta tccaattgac agtaatcctg aaaatgttct ggctgctgaa aatgcagaag
     4381 acctgatggc caattattgg atggatgtct atatgtgggg aaaatatccg attgctgcca
     4441 tgaggttctt agaagaaaaa ggatgggcac caacgattga agctggcgat gcagaattgc
     4501 tagaatctgc taaaccagat tttcttggga tcaattacta tcaaacggcg acgaatgcat
     4561 tcaatccatt aaacggagtt ggtgctggaa aaatgaatac gacaggaaaa aaaggttcat
     4621 cagaagaaac tggagttcct ggaatgtata aaaaagttga gaatccattt gtcgaacgaa
     4681 caaattggga ctgggagatt gacccagaag gattaaggat cggactgcgt cgtatcacaa
     4741 gccgttaccg catgccggta ctgattacgg aaaatggctt aggagagtat gacaagttga
     4801 cagaagataa acaaatccat gacgactatc gaatcaatta cttacaaagc cacgtcaaag
     4861 ccatcaaaga agcaatttcg gatggagcag aagtcctagg ctattgcacc tggtcttata
     4921 cagatttgct tagctggctg aacggttatc aaaagcgata tggatttgta tatgttgatc
     4981 aagacgaaac acaagaagga tctttggaac gatataagaa agacagttat tactggtacc
     5041 aaaaactgat tgaagaaaat ggagagaacc tttagttcac atacttaaaa caagaaggta
     5101 cggctggata tggccgtgcc ttttcttgat aaaatcattt agaaattaag aaaaactgta
     5161 aaataagctg aaaaatgaaa gaaagcgtca gttctttcaa acaaaacttt agttccctta
     5221 cttttttttg tataatgaaa cttgatgaaa tgagggataa taaatggcac aaaagaaacg
     5281 gcctactaaa aaaaggaaaa agacgaaaaa aacgaaaaag caaaaacagc agcaggaaca
     5341 aatgctgacg attggtgttg ggtttgcctt gattttattt gctgtcttcg gctttttaaa
     5401 attaggtttt ttaggaatat tgattgcaaa cggctttcgg atcattgcag gaaatactta
     5461 ccaaatcctc tgtcttttat tagccgtatt aggattttgg attgtcatta agaatacaga
     5521 gttttcaatt ggtaaaaacc gacgctggtt tggcggcata ctcttttatt tcggtatctt
     5581 attattgtta catgcccatt tatttggcaa actccatact ggggaaccaa atatcatggg
     5641 gacgacatgg gatttgttag ccagtgatat aaaacaaagt caagtagaca ataacgttgg
     5701 tggtgggatg attggagcaa tcctttatca ttttacttac ttcttgattg cacagcctgg
     5761 aagttattta gcagcagtgc tattactggc aggaggagca ttcctgttca gtaatctgga
     5821 aggctatcag ctgttaaatg gtatccaatc aattggagaa cgagtacagg aattattgga
     5881 aggtgatcct gccaagcaag ctcgaaagca agcggcaaag gaagagagaa tgaaacagcg
     5941 cgcagaagcg aaagaagcaa ggcgcttagc tgcaaaagaa gctgcagaaa aagaagctgc
     6001 agaatatgag aagaaaaaag cggtgagcca gcaaagggaa aatactgctg atgaatggca
     6061 ggaaccagaa aatacagagc cggagcagct aagttttgtt ccaatcgaca gttttcaaga
     6121 aaatatccat cctgcgaatc tagagaaacc agtaccagat acaccaaaac agaccaatac
     6181 agcagaaggt ttttcagatg agttgccaga ggatgacggt acctcattag aatttgagat
     6241 tgaagcagaa caggaaaatc aagactatga attaccgaca gttgatttgc ttgattctat
     6301 accaactgtg gatcaaagtg atgagtataa aaaaattgaa aaaaatatcg gcgtattaga
     6361 acaaactttc caaagtttcg gtgtcgatgc caaggtcgtc aaagccagct taggaccatc
     6421 cgtcacaaaa ttcgaagtcc agccggctgt aggggtaaaa gtaagtaaaa ttgtgaactt
     6481 aacagatgat atcgcgcttg ctttagcggc gaaagatgtc cggatggaag caccgattcc
     6541 aggtaaatca ctgattggta ttgaagtacc aaatggaaag atcagtatgg tttcctttag
     6601 agaaatcatc gaagcacaac caaaccaccc agataaatta ctagaagtac ctttaggacg
     6661 tgatgtttct ggacgggtac aaacggctga tctttcgaag atgcctcact tactcgtggc
     6721 tggttcgact ggtagtggga agtcagtagc aattaatggg attatcacaa gtatattaat
     6781 gcgtgctaag ccccatgaag taaaactgat gatgatcgat ccgaaaatgg ttgaattgaa
     6841 tatgtacaac ggcatacctc atctgttgac gccggtcgtg acgaacccta gaaaagcagc
     6901 gcaagcctta caaaaagtag tacaagaaat ggaagaacga tatgaaaaat tcgcggcaac
     6961 cggagtaaga aatatttctg gttacaatga atttgttcaa cagaaaaact tggaaaacgg
     7021 aacgaaacat ccaaccttac cattcatcgt agtgatcgta gatgaattag ctgatttgat
     7081 gatggtagcc agcaatgaag ttgaagatgc gattattcgt ttagcgcaaa tggctcgggc
     7141 agcggggatt catatgatcc tagctactca acgtccaagt gtggatgtta tcacaggaat
     7201 catcaaagca aatgtacctt ccagaatggc atttgcggta tcaagcggta cagattctcg
     7261 gacgatcatt gattctaatg gggcagaaaa gctcttaggg cgaggagata tgctattctt
     7321 gccaatgggt gagaataaac cgatccgtgt gcaaggagca ttcatttctg accacgaagt
     7381 agaacgggtc gttcaatttg ttacagatca gcaagaagct cattatgaag agaaaatgat
     7441 gccgacagat gaagttgaaa cgacaggtgc tccagaacag ccacaagatg agctgtttga
     7501 agaagcaaaa gcacttgtcg ttgaaatgca aacggcaagt atttcccttc tccaaagaag
     7561 atttcggatc ggttacaacc gagcagcacg tttagtagat gaactggaag ctcatggagt
     7621 tgttggcccg tcagaaggaa gcaagcctcg gaaagtattc attgagcagg aagaaacatc
     7681 agaaacgttg ccggacgata atgacatgta aaacttgtgt gatgaagggt tgaaaaacga
     7741 ataaccaata aaaagacaag gcatgatgat accaaaagaa aaaatggttt ttttccatct
     7801 tttctaatac ggtatgatgt cttgtctttt tagtttttgt gagattaaag ttctattcga
     7861 tttatttgtg aagtattgca ttaaggaaat gaatcatgta tagtatgtag cgaaactttg
     7921 agaaaaagag ggaaaatact ttggaaaaac agtccattta tggcttgacg aatgaagaat
     7981 taatcaattg gtttatagaa aacggcgaga aaaaattccg agctgcccaa gtttgggaat
     8041 ggttgtatca aaaacgtgta agtaatttca cagaaatgac gaatctatcg aaacaattga
     8101 tcgaaaaatt atcggagcat tttatcatca atcctctaaa acagatggtc gtccaagaag
     8161 cttcggatgg tacagtaaaa tatttgtttg aactccctga taaaaacatg attgagactg
     8221 tactgatgag acaagaatat ggcttgtcag tttgtgtgac gacacaagta ggatgtaata
     8281 tcggctgtac gttttgtgca agcgggctgc tcaaaaagaa ccgtgatctg actgctggag
     8341 aaattgttgc acagatcatg atggtccaac attattttga tgagcgcaat cttggcgaac
     8401 gcgtttcaca tgtcgttgta atggggatcg gtgagccatt tgataattac gacaatgtaa
     8461 tggacttttt gcatatcatc aatgatgcca aaggattggc aattggtgct cggcacatta
     8521 cagtttccac tagtggatta gcacacaaaa tcaaggaatt cgctaataac ggcttgcaag
     8581 tcaatctggc tatttccttg cacgcgccga ataatgaggt gcgaacatcg atcatgcgga
     8641 tcaaccgaag tttcccaatt gagaaattga tggaagctgt ggatgagtat ttagagaaaa
     8701 caaaccgtcg aatcacgttt gaatatatca tgttgaatca agtaaatgat agaccagagc
     8761 atgcgcaaca attagcagat ctattaaaag ataagaaaaa attagcatat gtcaacttga
     8821 ttccatataa tccagtcagt gaacatgatc aatattctcg cagtccaaaa gctgatgtat
     8881 tgagatttta tgatgtattg aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg
     8941 gtacagatat tgatgcagct tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg
     9001 ccaaataatc gtatcctact cgttttttca attaccataa aaggagcagt tttcttaatt
     9061 aaggaactgt tcaacttaaa taagagaaaa gattctatag cgaaagctat aaagcttttt
     9121 ttgacaagaa aaaccctcat caagcaaaga aatgtcctca atttgatgac agcctgcttg
     9181 atgggggttt tagcaattat ttaactgatt ttcttatatc cttgctggct ttcttgtgta
     9241 gcatgataag tttctacgtc aagaatggcc tgtttaaatt tttctcgggt gatttcataa
     9301 ggcacatgtt taatatcatt tgttgccatg gctttatcta agattgcttc taaatcggtc
     9361 gtgacattga gattcatgtc ggttaacgat actggaagtt ggttcttttt cataaacaca
     9421 tagattttat caaacaaatc gaactgctga tccatcatca ataaggtcag tacaccataa
     9481 ctgacagatg ctccgtgaag gtatccctcc attttttcta gaacagtcgt accgtaatag
     9541 aatgagtgtg ccatagcaga attatagtta ttgttaacta aaacagaaac caatccggta
     9601 gtaacaatga tatccgaaac gacttgttca attgcatatg agggacgatc gtcggcacta
     9661 tctttcaatg cttgcgtacc ataagtcaag agacgatcaa tgcacatttt tccgatttgg
     9721 attcctaatc tatccgtgta gcctagatca tcgccacgag cagaaaaagt agattcgact
     9781 tctttggata aagcatcgcc aatacctgcc cataagtatt tatcaggtgc ttcggcaata
     9841 attgctgtat caatgaaggt gtgtacaggt ggttttgaag gaaagaagag tccgtccatt
     9901 acgccttctt cgttatagat gacacatacc gcagtgacgg gcgaacagtt cgaaccaata
     9961 gtgggaaagg tgaagactgg tttagctagt ttttcaccaa tgacttttac agtatcgcaa
    10021 gcacggccac caccgactgc aaacaggacg tctgcttttt ggacactttc atgctggatc
    10081 aactgttcaa tatttccata agttgaatta cccccgtacc agagactggc gacaacctct
    10141 atttcctgtg tcaaagctac ttttattttg ttttctgcct ttgaaagtgc agtcttccct
    10201 ccaatcagca ctacttttgt cccatattct ttgacgattc catttgcttt ttcatacgca
    10261 tctgttccaa tggtataatt tggtaagaaa acactttgac tcatattcat ttccttcttt
    10321 cttcgttgaa acgggttaaa attttaaaga gtgatcgatc tgactgcggc tgtagatgga
    10381 gtagaggtga acctaagaca ggataagtga tgaataaggt ggcatgaaca ccttgaaatc
    10441 atcaataagt atcctaaaaa cgaacaagta caaaaacatt gaaaacgaca ataatgaatt
    10501 tttgttcctc ttagtatttc taccgattcc ctttgctttt ttgtaattat ctaaaaattc
    10561 taaaaattaa gattattata tgaaaacagg ttttatttgt caattcataa ttggtttttt
    10621 ttaaagcaaa agataatcta tatcatgatg aacaaacaga gttttttcct ccaattgatg
    10681 gctttcctga tctttcctat tttgaataaa gtgtttctgt ataatttttt tattttataa
    10741 gtaagataaa taatgaaaga atagagatac tgattggtag ctaaagattt ttgagcgaga
    10801 acatttcgtt ttttcaaaag gactgttaga ataagcaaga acaaggggga agacgtatga
    10861 taatcagtga atttgaccgt aataatccag tattgaaaga tcagctttct gatttactga
    10921 gactgacttg gccggaagaa tatggagaca gctcggcaga agaagtagaa gaaatgatga
    10981 atccagaacg aatcgcggta gcagcggtag accaagatga gttagtagga tttattggtg
    11041 caatccctca atacggtatc acaggttggg aattgcatcc attagttgta gaaagctccc
    11101 gacgaaagaa ccaaataggt actcgattag tcaattactt agaaaaagaa gtagcttcca
    11161 gaggaggaat cacgatttat ttaggtacgg atgatttaga ccatggaaca acgttaagtc
    11221 aaacggacct gtatgagcat acatttgata aagtggcttc catccagaac cttcgtgaac
    11281 atccgtatga attctatgaa aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa
    11341 atggctggga caaaccggat atttggatgg caaaaacgat tattcctcga ccaaattctc
    11401 aataataaaa agaggttgtg acagaagtaa tcagcttcaa gaaataagaa ggaatttacg
    11461 aaaattactt cttgaatttt tgtgaaattc caacttattt ccgaagaagc tacttttgga
    11521 acaccgtgta ccagaggttg tgaaaaagtc gcttagtttc gagtagtaag aaaaattttg
    11581 tgaaaatggc tttctacatt tttgcataaa tttggcttaa taccgagaaa ctggcttttg
    11641 aacaccgttt attcagataa gggactgtga caagac
//
LOCUS       DAJSKS010000085        11445 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJSKS010000085 DAJSKS010000000
VERSION     DAJSKS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.087"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            185..1183
                     /locus_tag="ON396_001887"
     CDS             185..1183
                     /locus_tag="ON396_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3113967.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="ON396_001888"
     CDS             1295..1804
                     /locus_tag="ON396_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR3113968.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="ON396_001889"
     CDS             complement(1834..2493)
                     /locus_tag="ON396_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HCR3113969.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="ON396_001890"
     CDS             complement(2495..3259)
                     /locus_tag="ON396_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCR3113970.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="ON396_001891"
     CDS             complement(3267..3944)
                     /locus_tag="ON396_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCR3113971.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="ON396_001892"
     CDS             complement(3981..5366)
                     /locus_tag="ON396_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR3113972.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="ON396_001893"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ON396_001893"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ON396_001894"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ON396_001894"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR3113973.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="ON396_001895"
     CDS             6305..7162
                     /locus_tag="ON396_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3113974.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDGTEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="ON396_001896"
     CDS             complement(7243..7578)
                     /locus_tag="ON396_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113975.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="ON396_001897"
     CDS             complement(7645..9015)
                     /locus_tag="ON396_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HCR3113976.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="ON396_001898"
     CDS             complement(9093..9629)
                     /locus_tag="ON396_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3113977.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9938..11419
                     /locus_tag="ON396_001899"
     CDS             9938..11419
                     /locus_tag="ON396_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3113978.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         3517 a         2300 c         1855 g         3773 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcgctatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatggtactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc
     9901 taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaaa
     9961 agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac aagaattatc
    10021 caatgactta catattccaa aacggacaat caaagaactg atccgaaaaa taaatgtatc
    10081 actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag gagagatcca
    10141 aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa aattatgcta
    10201 cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc cacaaactgc
    10261 tgtaccaaaa aaatatttac ttgaacaact ttatatatcg ccctcctatc tcgaaaaact
    10321 gacaaaacag ctcaatcatt ccttaaaaaa attccattta cggatcctat ctgtaaaaaa
    10381 ttgctgtact cttgaaggaa atgagttatc tatccgtctc tatctttact tttttctttc
    10441 agatacattt ggaggtaacg attggccatt ttcctcactt gaattaaatg cactaaaaat
    10501 gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa gaaaaaaata tctacttgct
    10561 gttaacttta ttcatattac gaaacaaaca acaggcatat tttccaaaaa cttcttctga
    10621 aatcactgaa ttgatggact tattgcagca tacacatgat tattcagaat actttacttc
    10681 tttttcagtg agtcgccctt gtgaagagac agaaacagag agagcctact ttaattattt
    10741 tttctattgt ttttttccaa atcaaatctc ttttcgacaa aaacaggaga ttggtttttt
    10801 attttccaca agcaaaaacc ttatttgtcg acaagtcggt caattgatga cttacttaac
    10861 tcaacagttt cctctcaacc atttagaaaa taaaatacca atttattttt attttcttgt
    10921 gttggcagtt gtttttttcg aactgttggg tgaatccagc agtagttttt ctacactttg
    10981 ctttccagaa gccacgtcca atcattctag ttcttttata caggaaaaga aattaagaga
    11041 aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac caaatcagtc aataccttag
    11101 tcaattgatc caacacttac tacaaacaga aacgaaagag cagttactca tccatttgca
    11161 gataaccaaa cagtttacag gaaattatca tcttcaaagc catttgcagc agttatttcg
    11221 caaagatgct gttaaaatta ccgcatattc agaacatgct gatttgatca ttacagatca
    11281 gcatgagaaa atttcagatg aaacggagac tttctatctg gatacaacgc acaatacagt
    11341 gttatttggc aagttaataa aagcggttca tgaactgtat atgaaaaaag gttatgagaa
    11401 caaaacaaag aggatgtgac aaaattcttg tcacatcctc ttatt
//
LOCUS       DAJSKS010000086        11390 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJSKS010000086 DAJSKS010000000
VERSION     DAJSKS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.088"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            34..1674
                     /locus_tag="ON396_001900"
     CDS             34..1674
                     /locus_tag="ON396_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCR3113979.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            1828..3150
                     /locus_tag="ON396_001901"
     CDS             1828..3150
                     /locus_tag="ON396_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HCR3113980.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3212..3643)
                     /locus_tag="ON396_001902"
     CDS             complement(3212..3643)
                     /locus_tag="ON396_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3113981.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3707..4543)
                     /locus_tag="ON396_001903"
     CDS             complement(3707..4543)
                     /locus_tag="ON396_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR3113982.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4733..5035)
                     /locus_tag="ON396_001904"
     CDS             complement(4733..5035)
                     /locus_tag="ON396_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCR3113983.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5061..5492)
                     /gene="ruvX"
                     /locus_tag="ON396_001905"
     CDS             complement(5061..5492)
                     /gene="ruvX"
                     /locus_tag="ON396_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR3113984.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5489..5755)
                     /locus_tag="ON396_001906"
     CDS             complement(5489..5755)
                     /locus_tag="ON396_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCR3113985.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6009..6734)
                     /locus_tag="ON396_001907"
     CDS             complement(6009..6734)
                     /locus_tag="ON396_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCR3113986.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7100..7170)
                     /locus_tag="ON396_001908"
     tRNA            complement(7100..7170)
                     /locus_tag="ON396_001908"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7136..7138),aa:Gly,seq:ccc)
     gene            7287..8039
                     /locus_tag="ON396_001909"
     CDS             7287..8039
                     /locus_tag="ON396_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCR3113987.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8055..8534
                     /locus_tag="ON396_001910"
     CDS             8055..8534
                     /locus_tag="ON396_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCR3113988.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8785..10365)
                     /locus_tag="ON396_001911"
     CDS             complement(8785..10365)
                     /locus_tag="ON396_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCR3113989.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10358..11101)
                     /locus_tag="ON396_001912"
     CDS             complement(10358..11101)
                     /locus_tag="ON396_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3113990.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11277..>11390)
                     /locus_tag="ON396_001913"
     CDS             complement(11277..>11390)
                     /locus_tag="ON396_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCR3113991.1"
                     /translation="RVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
BASE COUNT         3458 a         2277 c         2040 g         3615 t
ORIGIN      
        1 ttcgtttgta ttaaaactta ggaggaatca ttcatgctta cagggctaaa cttattaaat
       61 aatccttttc taaataaagg aacagctttt acaaaagaag aacgggcaaa atacggaatc
      121 actggtatgc ttccaagtac tgtccaaaca ttggaacaac aatctgtcca agcttacggt
      181 caatacttaa gcaaacagac cgatttggaa aaacgtatct tcttaatgaa tctattcaat
      241 acgaaccgga cattgtttta taaattgatg ggcgaacatt tagttgaatt catgcctgtc
      301 gtttatgatc ctgtcgttgc ggattcaatc gaacaataca acgaaatttt cttgaaacca
      361 caagatgctg tatttctttc aatcgacgaa ccagaaaata tcaaagcaag tttgaaaaac
      421 gcagcagatg gacgcgacat tcgtttgatc gttgtgacag atgcagaagg aatcttaggt
      481 atgggtgact ggggtgtgaa cggtgttgac atcgctatcg gtaaattgat ggtctacact
      541 gccgcggctg gtatcaatcc tgcacaagtc ttgcctgtat caatcgacgc tggtactaat
      601 aacgaagaat tattgaataa tccattatac ttagggaatc gtcatgctcg tgtagaaggc
      661 gaaacttact acgaattcat tgatcaattc gttcaatcag ctactgaatt attcccagaa
      721 ttgttattac attgggaaga ttttggacga ggaaatgctg caacgatctt agaaaaatac
      781 gaagataaaa tcactacttt caatgatgat atccaaggca caggaatcgt tgtactagct
      841 ggtgtattag gtggattgaa tatatcaggt gagcaattga aagatcaaac gatcttgaca
      901 tttggtgctg gtactgctgg tgtaggtatc gcaaacatcc ttttagatga aatgatccgt
      961 caaggtgtac ctgaagaaga agcacgccaa catttctatc aagtagataa acagggttta
     1021 ttgtttgaag atacaaaagg attgactcct ggtcaaatcc cattcgctcg taaacgctca
     1081 gaattcacta atgctgatga attgacaaac ttagaagcag ttgtaaaagc aatccatcca
     1141 acgatcatga tcggtacttc tacccaacct ggagcattca ctgaagaaat cgtgaaagaa
     1201 atggcggctc ataccccacg tccaatcatc atgcctttgt ctaatccaac aaaattggca
     1261 gaagcaaaag caaaagattt gattgaatgg acggacggaa aagctttagt tggaacaggt
     1321 atccctgctg ctgatgtgga atataacggt gtaacttacc aaatcggtca agcaaataat
     1381 gcgttgatgt acccaggttt agggcttggt ttgattgctt caacagctac acgagtaaat
     1441 gctgaaatca tttctcaagc tagtcgagca ttaggcggaa tcgttgatgt aacaaaacca
     1501 ggagcagcta tcttgcctcc agtagcaaaa atcactgaat tctctcaaac gattgctgaa
     1561 actgtcgcaa aatcagttgt tgcacaaaaa ttgaatcgtg aagaaatcac ggatatcaaa
     1621 gaagctgttg aatctgctaa atgggttcct gaatataaat ctttagaaga ttaagattgt
     1681 tatagtaaaa gaacatacag aaactttgta tgacgtctaa aaagaaaaaa gcgactgcgg
     1741 caaaacagca gccagccaac tactgctctt ttgccgcttc tctttggctt ttttcatacg
     1801 atacagaata agaaaggatc tgtaaaaatg gcacaaaaaa caacaaccgc aactgcagtt
     1861 gaggaaaaga aaaaagaagg gttttggtca acaaaaatta ccggcgtaag tttgcccctt
     1921 tatttgatca tggtccttgt cctgatcatt gcaatggctt taggaaaatt accgacgaat
     1981 atgattggcc caatcgctgt tttggtcatt ttcggtaatt tatttcattt tatcggaaca
     2041 aaaatcccta tagtcaaaag ttatttaggt ggtggctccg tttttgcgat ctttgcctct
     2101 gccgcgattg ctacatttgg gatcttacct gagtatgtag taaaatccac agaaaacttt
     2161 gtcagcaata tgggcttttt agacttttat attgctgcct tgattactgg tagcatcctt
     2221 ggtatgaacc gtaatctatt gatgaaagct tctgttcgtt ttattcccgt tgcacttatt
     2281 tcaatggtcg tctccttttt tgccgtagga ctcgtaggta tgcttatcgg aaatggtttt
     2341 gcaaattccg ttttatatat ctcattgccc gcaatggctg gtggtgttgg agccggagct
     2401 gtacctttat caacgattta tgcaaatgtt ttaggtacgg acgcttcttc tatcatttcc
     2461 cgcctgattc ctgcgacagc aatgacaaat gtcctagcta tcattggtgc agcgttagtt
     2521 gctcgtgcgg gacaatcaat gccaaaattc aacggtaatg gtaaattaat ggaaaaaggc
     2581 gaccttggcg atggaaaacc tagaaatgta aaaccttcca ttccacaatt aggtgtcggt
     2641 ttgatggtat cattgacttt cttcatctta ggacaaatct gtaactattt tgttcctaaa
     2701 gtccatgctt acgcattcat gatcattatc gttgttatct gtaaaatcgc aaacctatta
     2761 ccagaatatt acgaagatgc agctatcatg ttcaacaact tgattgtcaa aaacttgaca
     2821 gcagctgttc ttgccggtat cggtattgcg ttattgaatc taaatgtatt ggcttctgct
     2881 ttgacatggc aatttgttgt cttatgtttg acaagtgtta tcgcgatttc agtagtttca
     2941 ggatttgtcg gtcgtttgtt tggtttatat ccaatcgaat caactattac tgctggctta
     3001 tgcaacaact cgatgggtgg tacaggaaat gtagctgtct tatctgcagc taatcggatg
     3061 gaacttattg cttttgctca aatgggcaat cggcttggtg gtgccatcgt tttgatcatc
     3121 tcaggtttct tgatgcaatt actttcttag tcaaaaaaaa tacctgatca tcaagtatcg
     3181 gcttgatgac caggtatttt ttgataaaaa tttacaggat tttcaaaaat cttttttgca
     3241 ttttatcgga atgataaccg atgcgttcat aaaatcggtg tgcttctact ctgtattctg
     3301 cagaattaag tcttattgct gatattcctc gtcttttcgc ttcttctacg ccagtcatca
     3361 aggcctttcc gattcctctt ttttcagcat gtttagacac agcaagtgcc ataatattga
     3421 acatcggatc gctgaaaatc gattcatagg actccgcttg tacatagcca gctgcttttt
     3481 tagaagtagg atcttcatag accaagaaaa aatttttttc tggctccttc aacagtttct
     3541 tcatttgtct caccgtaacg gttaatggta catcatatcc taattgttcc gcattgattt
     3601 ctcgtagtgc ttctgcatct cctaaacgca tttcccgcaa catccaaatc accagccaat
     3661 ttttattttt ttaaatgaat ttttttatca cagtatactc cttttttcac ttaaaaaaag
     3721 acttttcagt gattttgact tgcttgaatc cagatccttc taatgtttgc aagatgacga
     3781 aagcattgta aagattgatc aaaagaacca cgatgacaat cgcttgaaaa ataaaggtca
     3841 ctttttttgg agacaaaacc ctgctaaatc ttgctcccca caaacctcca ataacagcag
     3901 cggggataac ataaaacaac atagataaat caaagcgcat aaagctactt gttaaagcaa
     3961 tcgttaccaa ttttgccagt tgagaaaaga aaatcgtaga tagagaatat agtgttgctt
     4021 cttttattgg caatgcaaac atcagcatca acaaagaaac attgactggt ccaccaccaa
     4081 tacctaaaaa actagctaaa aagcccagaa cccccccaca tataaggtac caagtccatg
     4141 acgtcaaatg aaattttggc tgatgatgct tggtgtagaa gaacgcaaaa accaaggtca
     4201 ataccgtcaa aaaaatttgg atcagctgca catgcttctc gttttcaaat aactgtaaaa
     4261 aaacttcgaa tacaatgttg ccgactactc ctccaacaac agctccacta gatacccaac
     4321 cgacaatctg ccaattcagt gatttcccgc ttgatacttg tcgcacagtc gaaacaatgg
     4381 acatcgtgaa taccgcaact gttgagtaaa aagaaatccc tgcaacagaa tgggcgccga
     4441 taagatcaag aatcggtttg atcaacacgc ctccacccat cccggaaata gcgccaactg
     4501 tgttcgccag aacgatcacc acaaaataaa tcagaccaat catcttgtat ttcctttctc
     4561 ttgtttatat cttttagcga ttgttactct tttcttttat tatacagtaa aagaaacaaa
     4621 cgaggcttcg aaagcttttc tactttttaa ggaaatttag tgctaaacga aaaaaccggg
     4681 ctctactttt cagagagtcc agcttttctt acaccttatc gattcttttg ttctattctt
     4741 cttccgccat gaatgtattg aatacttctt cgatcatatc ccattctgcg tctgtttcga
     4801 tttgttctaa ctcgccttct gttccatctt cgttttcgat gtatgcataa gcttgtaatt
     4861 ctacatcttc atcttctgaa acaccagctg gatataaaag aacatagtta cgtccaaatt
     4921 cttcttgtcc gtcaactgtt agcaagattt cgtataatgt ttcatttcct tggtcatcca
     4981 ccaatgtgat gtgttcgtgt ccttcgtgtt cgtgggaatg gttatgttct gtcataagat
     5041 tacctctttt ctttttatta ttaaaataat tgcttacctg agccgtctaa ataattttgt
     5101 aaaatcatca cagctgctaa tttatcgatg actttttttc gtttcgctct tgaagtattt
     5161 gcctgctcta ctagcatacg ttctgcctga acagtggtta gtcgttcatc ttgataatca
     5221 accggcaagg agaatagttc tttgatcttc tctccatatg acatggaagc ttctgccctt
     5281 ggtccgatgg aattgttcat attcttaggt aatccaacta caaaacggtc tacttcgtac
     5341 tgcgccacta attccttcaa ccgatcaaaa ccaaactctt cttctgcctc attgattcga
     5401 atgatttcta ctccttgagc tgtccagcca aagggatcgc tcacagcaac gcctactgtc
     5461 ttagaaccga catccaagcc cattactctc ataaattcac tccatgattt gagaggtagt
     5521 ctttgacaat tacttccatg atttcatcgc gttcgtgacg gcgaatcaaa tttcgtgcat
     5581 cacgataacg aggaatgtag gccgggtctc cagaaagcaa gtagccgaca atctgattga
     5641 ttggattgta gcctttttct tccaaagccc gataaacaat cgctaatgtt tcgctcactt
     5701 cttttttgcg actatcgtca aaaccaaaac gtacggtttc atctgtaaaa cccatgattc
     5761 tacacctctt tcccgcaagg taattccagc ttaccttcta cacccttatt ttactcgaag
     5821 aaagggaaaa ctacaaacaa atattccaaa cttctgaata aattaagaaa cacgatttgt
     5881 ctgatttttc cttttttctg tggctttttc aaaagccctc tttccataaa gtatacgcta
     5941 tatttctttg aaagtatgtt tttacacaat tttttcataa agttcataaa aaattcgaat
     6001 tgctgctact aatttttcgt tttcgttgct tgccctgtct cataattgat ggtgtagcca
     6061 tcttgaacat tgtagataaa gacattgaat tcaatttttt tgtcttcaat gctttctgct
     6121 tccaactgca cacctcttgc taccagttca tttccctcga aatgaggagt gacacgatag
     6181 cgtacatgat gatttgtttc cttgatatat gccgcaatat cattttcgtg ggcaagcatg
     6241 tacggagcat tcaacgaacg tgtccctgtc attaagtttt taatgttatt attttgacca
     6301 gtcaactgga aaccaatcaa atggctgcgg ttgtataacc attgtccgtc acttaatttc
     6361 ttttgtttcc aacctgtcgg atcaatatac aaaggttctc tcttttcagt aggaaacaag
     6421 tcttttccca acatcgcatt ggcttgtcct actctgttca atttgtctat atttgaatag
     6481 ctttcccatg tccctttgga aagactaaga tcctcttttg taaactccgg ctgattccca
     6541 ttgatttcta tttcttgatg ttttccatca tattctggca catccgctac ttgagaagct
     6601 tgcggtgtgg aatcaaaaat ggttgtaatt tgttggaaga tatctgaatc tttttgtgtg
     6661 gtggaaatac cgagtgcccc tgcaatcaca gcagcgacga tccccacgat cgccttggtt
     6721 gtttttttgc tcatttcttt actcctttac taacagttcc ctaaatctta acatatgttc
     6781 ccctataaat aaatactgga aagaaataga aacagtaaaa aaataaaacc agtttttaga
     6841 cggcttaaga atacaaaaaa agagacggat tcatcgtctc tactcaatca caaaacacta
     6901 tttacaaggt atttattatc attcaaaaaa tttttcttaa tactcaaaac taaacggctt
     6961 ctgtacgacc tcttggtaaa cagtgttcaa aagcttgcat ctgcggtaat aagtccaacc
     7021 aagtcaaaaa cataagaagc ttttttgctt gactattctt attacttgat gcagggcagc
     7081 tttttcacag cctcttttaa gcgggtgacg ggaatcgaac ccgcgacatc agcttgggaa
     7141 gctgggattt taccactaaa ctacacccgc gatacaaaaa cagtatacgc ctctttcact
     7201 ccttcctcaa gtttttttat tctttacgct tccgatatgt tttgtagtaa aataaaacta
     7261 atcaatcaat tattggagaa gatgctatga cgggaaaaga attagatctg ttgctggaca
     7321 cttgtttact cgctgggaaa atcatgatgg aaagcaatgc ggagatgtat cgggtagaag
     7381 atacgatgag tcgaattgct ttagcttctg gaaatttccg tttggtcagc tatgtcactc
     7441 aaaccggtct ctttattggt cttgatcgta cctcgaccat ccgaatggaa caaattacca
     7501 accgttcgat taatttagaa aaagtagtaa aagtaaatga tctttcacgt aaatatgttg
     7561 cttcagagct aacattagaa gaactttact gcgaactgca agcaatcgac cgagaccgtt
     7621 ctttctttcc tgtctggttg caaatcgtta gcgcggcagt gatcagtgga acaattatgg
     7681 tgttatttgg cggaaagatg accgatttac ctgtgacttt agtcattgga ggattgggat
     7741 acgcagctta tctatatagt ttgaaatttt ttagaatcaa atttttatcg gaatttttat
     7801 cttctttatt gattggcata gccgctatct acagcgtacg ttttggctta ggaagcaatc
     7861 aggatctaat cattatcgga tgtgtgatgc ctttagttcc tggagttcaa attacgaacg
     7921 ctatccgtga tttgctagct ggacattatg tctcaggagt ttctcgcgga acagaagcga
     7981 tgatgactgc tacgatgatc ggttttgcga ttgcttttat tttccagctt ttttattagg
     8041 aaaggaacac aagtatgggg acgatattta ttcagttttc ttttagtttt ttagcgactg
     8101 cggcatttgc cgttattaca aatgtaccta ggcgttcatt gatttgttgt ggtctttctg
     8161 gtgcttttgg ctggatgatc tactggatct gtgttcaaat aggcggatct gctgcttttg
     8221 gttcattgct tggttcttta ggagtagcat tcatcagcga tcttttctct aaacgtttga
     8281 aaatgcccgt aacgattttc aatattcccg ggatggttcc tctagtccct ggtggtttag
     8341 cttatcaagc ggttcgaaat ctcgttactg aatcctatcg agaagcggct cattatgcgg
     8401 tccaagcaat tatgattgct ggagcaatcg ctttaggtct tgtattatca gaagtcttta
     8461 accataacat ccgtaatttc aaagtaaagc gtgacgcaat catgatgaaa cgaaaaaaac
     8521 ggctttttaa ataaacgaag tgtaacaaag aaaaggttgt gaaagagacg ttcagccttg
     8581 agcgatgaca aaatatctca caaacattgc gacaagtaac cgcaggagca ataagaagta
     8641 attatgagta ttaaaaaaag ttttgtaaaa atagcttctc acatttttac ataaaattga
     8701 cttaatactg agaaactgac ttttgaacac cctctatctg taaaaaaggg gctgcgacat
     8761 gatttttgtc acggcccctc ttctttattc aaattttttc caaaacgttc gttgataata
     8821 aacgatccat agagcggaaa gtattagaac taaaccgatc acaggaatat tcactaacca
     8881 aaatggataa aacaaagcca acattccgta taagacgata agaataatac tgatgatcaa
     8941 tgaacccatc atagttgcga ctaaacccaa acttcctgaa cctctgttaa ataattggtt
     9001 aatattggtc cagttcagca gcagcaaccg ataatctcta gcgaaataac gtagtgacaa
     9061 taagtagcag ccgaataaag aaccaaatag caagctaggg ataaaaaaca aaggcagctg
     9121 aaaagacagt ccgcttaata atgcgatccc accagttaaa aaactctgca atatccatcc
     9181 gaatctgaac ttttctttta aatattggtt catagagatt ggcaaagaac ggataaacag
     9241 aaaattttct tgatccaaag aaataagatt gctgataaaa gaagtctgat taactgttaa
     9301 gaaagccaat gcgatcccta ccaggaaaac aacaccgatt aacttattat ctaaatcagt
     9361 taattttatt tcgcctccta gggcaaaagc aagaataaaa atgattggag ttaacaagga
     9421 agaagaaaga acttgcataa tcaaattagg ttcttttact aattgagcat tgtaactaaa
     9481 taaaagctga tgcacatttt ggttcttttt atgggttctt tttattttga actcactgtt
     9541 tgaatcagtc agttgctctg taagctttgg tactatcagc tgttttacag caaggaaaaa
     9601 tcccagagcc gtgatagcta gtattcccca acccaataaa ccttgttgag aaagtggctg
     9661 actggcagca tcatagaatg gcaataatat cgaaatcgga tgccgatctg ttaactcatt
     9721 ttcaatagtc cccgtttgat gattcatcca catgattcca gcaaatgcga tcagcatcga
     9781 gctgccaagc aagatactag aaactgtttt tctatgtttt ttaaacactg ctgttcgagc
     9841 caaaccaaaa acaatcaaac tagccagaca aaaaagaata atcaaaaata gagaaaacaa
     9901 aaggattgag ataatgatag taagcaaaat ccctagatca gctcgccatc cggttaaaat
     9961 aaacatgact agaagcggaa aaacaaaagg aatgatggtc aaagcaacta ccaaaatctt
    10021 tgctgaaaaa acctgttctt gtccaaaagg cagaggcaaa aatacttgca ggtcatttcc
    10081 ttcaaaaaag atattataaa tgactgatat actttgagtt aaacctaaca taccaaaaag
    10141 tgccacgtaa taagtaaaga aacctggcaa tttaacaaag ttcatagcaa acatagcaaa
    10201 tccgtaaatc agtaaaaaaa tcagccctga aaaaagattt tgagacaaaa gatagatagt
    10261 taatcgcttt cccttttttc ctttctctct tgctttattc gtgagctgtg gattaacata
    10321 taaaagatta actcgaatta attctaataa ttgtcgctta tacatacggt ccatctcctg
    10381 tcgcttgcct tccagccatt ttcaaataaa tcttttctag cgtttcatca ggtgagccat
    10441 tcaataattc actaactgcc ccctgataaa tcaattcacc tttcttcaaa atagccagtt
    10501 tatcgcataa ctgttgtgcg gtatccaata catgtgtaga aaaaatgact gtttttcctt
    10561 ttgcggcatg agccttcatc atttctttca aatcaaatgc tgcttgagga tcaagtcctt
    10621 gcaaaggttc atccaatacc caaatatctg gatctggcag caacgtgccg atcaaaatcg
    10681 ttttttgacg cattccatgt gaaaaacttg cgattgtttc attttgatga ctatacatat
    10741 caaaaagcgc tgtcaattct gctaaacgct tttctttttt ttgtgtgtct aattcatagg
    10801 cagctgaaat caagtcccaa tattctccag ccgtcaattg taaaaaaata tctggggaat
    10861 caggcacata accgattttt tgtttgatac tcaatcgatt ttccgtcaat ttcattccat
    10921 ctactaaaat cgttcccgat gatggttcaa taatactaac taaactttta atcgtagttg
    10981 attttccggc accattatgt cctaaaaatc caaaaatttc cccttgtctt atcgagagag
    11041 acaagtcttt taatgcttgt tttttcccgt aacttttact tacattgatg aattcaatca
    11101 tttctgtact ccttttgttt gttattactt tttattgtac ccttcgttat tggtggagta
    11161 aatgtcaaaa agagtgcata acaaaaaagg atagaaacga ctaggctgct acttgggttg
    11221 ttctcttcta caggaaaaca ctcttctaac aggtaagata tgagtctttg ttttctttaa
    11281 aatgattcat tttctatctt ttcttgcata aaattcttta gtttatcaat acttctcttt
    11341 cccatctcta gttgttctcc atttgcaaat gttactattt cgtttactct
//
LOCUS       DAJSKS010000087        11369 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJSKS010000087 DAJSKS010000000
VERSION     DAJSKS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.089"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            172..378
                     /locus_tag="ON396_001914"
     CDS             172..378
                     /locus_tag="ON396_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HCR3113992.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(580..1581)
                     /locus_tag="ON396_001915"
     CDS             complement(580..1581)
                     /locus_tag="ON396_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3113993.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1586..3499)
                     /locus_tag="ON396_001916"
     CDS             complement(1586..3499)
                     /locus_tag="ON396_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCR3113994.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3667..4173)
                     /locus_tag="ON396_001917"
     CDS             complement(3667..4173)
                     /locus_tag="ON396_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3113995.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4333..4773
                     /locus_tag="ON396_001918"
     CDS             4333..4773
                     /locus_tag="ON396_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCR3113996.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4799..5956)
                     /locus_tag="ON396_001919"
     CDS             complement(4799..5956)
                     /locus_tag="ON396_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3113997.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(5959..6315)
                     /locus_tag="ON396_001920"
     CDS             complement(5959..6315)
                     /locus_tag="ON396_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3113998.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6613..7587)
                     /gene="sgrA"
                     /locus_tag="ON396_001921"
     CDS             complement(6613..7587)
                     /gene="sgrA"
                     /locus_tag="ON396_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HCR3113999.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7783..8622)
                     /locus_tag="ON396_001922"
     CDS             complement(7783..8622)
                     /locus_tag="ON396_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR3114000.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8807..9694
                     /locus_tag="ON396_001923"
     CDS             8807..9694
                     /locus_tag="ON396_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR3114001.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10288..10983)
                     /locus_tag="ON396_001924"
     CDS             complement(10288..10983)
                     /locus_tag="ON396_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR3114002.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(10967..11365)
                     /locus_tag="ON396_001925"
     CDS             complement(10967..11365)
                     /locus_tag="ON396_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR3114003.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3391 a         2280 c         1701 g         3997 t
ORIGIN      
        1 atgatggtaa actttgtacc tatctgattg ctgaatcatt aattgcttat cattagccat
       61 ctttgtttca agttcgctag atgatcattt tattcacatc ctaacaaact tgtcacattt
      121 atagattctt aatctattta tcatacaatt ataaaaaagg aggtgctgct tatgaaagct
      181 atccatattt ctttgctttt tttcgctagt gcattgattt tatttgctat tcatctttgt
      241 tcccagcctt atatcaataa caacggaata ttagtggaac cagcattctt ttgcttacca
      301 ctcggctttt tgtcccttct tgcctccgca ggcttcggat ttgttgcttt ttttaaacga
      361 tcaaaacaac aaatctgact tataaaattt acgtaaaaga gatcgtgaca ataatcgtgt
      421 gatattccca aatttcgaat aaaaggtgcc ccttgccatc tctaactcct cagtcacaag
      481 ttacaatatt cataaaccat gaaaagcttg tttattgaaa ttgctccttg tgacttggag
      541 ctaagtgaca gattacaacc tcttttgcat cataagactt tactccttga aaaacattac
      601 taacttttga tatgtttctt gcgcttgttt attgctgaaa tcaaactgat attcatggtt
      661 caaaacagca tttttctccg cataatctac ttttgtgact ttcacccccg ctttctcaaa
      721 tacagaagtt acttcttcca tttgtttcgt aaacgtattt gtatttccat ccgttaagaa
      781 tgtgttttgc gtccaacgat ccgcatgtct gaccaaggct aaacttttga ccttctctga
      841 ttgttcaaag tccttattat caaagtaagc ccatccacat ttttcataaa gaaagctaga
      901 gattttatca cttacttgag taagtgcaga aggatccaac agaccagaca aagaaataaa
      961 cttcgtaatc tgcttgttct ccaaagtcgg atcaagatga tcagcttttt taatttcttc
     1021 attttgaagt gctaacaaat attctccact aatttgtgca cctgccgaat ttccaccaat
     1081 cgaaatattc gaccaatctg cttgatcttt tatctctttt ttagctgctt gaatggcttc
     1141 gttcaattgg ataactggtg taggatgttt tgcttccggt gatcgttcat aatctatatt
     1201 tacgatgatt tgctgcgtgt ctgaacatag atattccaaa taatcacgac aatcattttt
     1261 atcccctcct acatatgcgc ctccatgcat ccagaaaaga atcggtaatt ttttcttttc
     1321 cactttcggt gtgtagatat ccatattatt atttttgtac tttgaaggat aggatatttc
     1381 tttttttata tcgatttgtt cgattttttg ctggaaatca tctggtcggg catatacttg
     1441 ttctttttca cttgtgtcaa attgttttct aacaagcaag ctaatcggct ttggtgtgat
     1501 ttgattaatc aaaaatacag tgactaaaat aactacgatg ataactggta ctgcaatcac
     1561 tattcgcttg aaccatttca ttcatttacc ctctctttct tcgccaaatc catgtgataa
     1621 tcgtgctaac agataaaaat ccacctgaat aaatgagaag cgaatgtttc ttttctccag
     1681 ttttcggcaa cattcttttt tttgcatctg tctttttttg tactttcact gtcatctgaa
     1741 tcggttgcgt atcatcactt gattgatcct ctcgaggttc attctttccc ttattgtctg
     1801 cattctctgt cggatcttgc ttttttggtg gagctactgg gatttttttg taatagaaaa
     1861 taatagttgg tatcgtctca ttaaaaatcc cttcagtcgg tccttcaaca tttgctaatt
     1921 cgtatccttc gaatgtcttt tgctctgtca tataattttc tccataagct ccgtaaagct
     1981 ggatttcgtc ctggtctttg agtagacttt tattgttttc atccacatac tttacgttta
     2041 tctgatcatg gaacactact ttcaaaaagt ccgttacatg tttcgtgtag gcagcatggt
     2101 caacttcctg tgcctttcca tgccctgctc cttctactaa taaaagatct ttataccctc
     2161 ctgataactc atataatttt tgcgcgttag acacaggtac tacatcatct gctgttccat
     2221 ggataatcaa tttaggcaat gaatctgaca ttccaatcgt aggtagagca gcttttgtat
     2281 ccaaatgcat ctcctgatca aaatagtatt gtttcaaaag gcccataaat aaatcttcat
     2341 gctcttgatc cccaatgatg tctaactgat tttttatggc atccggtagt gcgggggcta
     2401 ctgtctggct gtacgtaaaa cgtagttgtt catcaattga tgcaaatccg caattttcaa
     2461 taattccttt gacattgaca ggaatatctt tcgctaatac agacatcatc gtagctgctc
     2521 ccatcgaacc accataaagt aaaatctttt gagatggccg ctctcgaact tcttgattta
     2581 tccagtaaag gacgtcatct gactcatatt gtccataggt cacataatcg cctccgctat
     2641 taccagtcgc tctggaatca acaaataata aattataccc ggccttataa aaatcattgt
     2701 attcttctgt aacgatcccg ttattccaat tccctcgaaa tccgccatgg atcatgactg
     2761 ttttatctga accttgatcg acataatatg catatacatc catcattttg ccatcttgtt
     2821 tgtttagtac agtttgatga agttgttttg tatttggata gaagcctgtg ttttctgtag
     2881 gcgttgtccc atcatctgta cctccaagca tcgacatcaa cacactgttt tttgcaaaag
     2941 cgatatcgta caatagataa gtagcaatca gatgtttgat aatctcttta tctacaccat
     3001 aatcattaac agcaagctca gtgactaata actctattaa ttttgacttg atgtcttctg
     3061 ttggtccatt aagtatttct gtcatctgtt taccgaagta attcaaatat tctttaccat
     3121 tgaaattttc acctttaccg atatcatcat tttctgtccc attctctttg tcacttactt
     3181 tagcagaaga ggattcgtcg tttgacattt ttgtagctgt tgcttcttct gtttgttggt
     3241 tatctgtggt agcggtcgtt tcttgatctg tgctagtaac agatgtctca accgttgttt
     3301 cagtatttgt ttctgtttgt gaagtactcg atttcgtttc atttccagca tcagtagttt
     3361 ctgtggtttc tacctcccga gattccgatt cggaagaaat gctctctcca tatactgtcc
     3421 attgcgggac gcttgtactt acagcaaata agatgaccaa cgctcccttt aatacttgtt
     3481 ttgttctcaa gctattcact cctttttatg tttctaggag aattatatgt aaacgcaaac
     3541 attttttata caaaaaataa atggtttatc ccataaaaat agaacaggat tattttctca
     3601 ttttacgtaa taaagcagaa tggataaacc agtatccatt ctgctttttt taaacgaaga
     3661 aacaacttat tgctccaaaa atgtcagaaa tcgtttatcc caaattttct cgtaaaaagc
     3721 gattgtttcc tttgctttca taaaaggatt atcataagtt gtcgttgctc ctctacacat
     3781 cataagctgg tatcctaatc catgagcaaa cttcacagtc gtatctatac aatactctgc
     3841 ttgtgcccca cataattcga tcgaatgaca gtccaattca gttaataaaa cactcaaatt
     3901 agtcttataa aatgaattcg catgggtttt tctcacaaaa taactgtgct caggaataga
     3961 taattccgac aatagctccc atggttttgt ttgtggcact aattgagcat cttcgtgttg
     4021 aacgaaaata actggtttcc caatcgtttc gtatgttttg attcttttat tcactaatgc
     4081 tgtcactgtt tctaattcgt gtatcggcga agaatcaaaa catacccctt tttgtaaatc
     4141 gatcactact attgcgtcgg ctgtgtttga catacttgtg ccttctttct tttttcttga
     4201 tttaatagta tttcttatct agctagttcc attatacgtg acaacatttt cttattttac
     4261 acctctaaat ggcacttttc gactatttct tcatgttatt atttcataaa tacttagcta
     4321 ttggaggaat gtatgatgat taaattgtat acaggttctg gctgctcgtc ttcaagacgc
     4381 gcaaaacgtt ggcttatcgt ccatgatata ccgtttatcg aacagaactt aattcgtcaa
     4441 ccactaacca aaaatgaatt tatccactta ctaaaattga caaacaacgg cgtgaccgaa
     4501 atgctctcta ctcattctat tagttataaa aatcttgttc acagaataga tgaactcagt
     4561 ttgagtgaac tatatattga gattcgtaca tccccttctt tattgaaaaa accgattatc
     4621 tacgaccaat ctgcaaaact tttgactgga tttagcgaag ataaaatcag gatgttcctt
     4681 tctaaaacta aaagaaagca agagctcaac gaactcttgc tttctggtca tcttgtcgat
     4741 tttaaagaaa ataatctttt tgcaatgtct taattttggc ataaacaatc tgcttttctt
     4801 aattaacgaa atccaatttt tccaatatgg atctattttt ctcattcctg atttttatga
     4861 tttcagcttt cagacgcgcc caatcatttt ttgtaggttg tccattccag taaaatatct
     4921 gtgtattcgt ttgatcatat gcctttttgt cgataggata atcgctaatg acaatatcag
     4981 gtaactgatc agcggatgca ctatatttta ttgggcgttc agtcatagat aatatccttt
     5041 tctttgccca taattcctgt gttttaccat actttgattg aatatagaat atgacaggtt
     5101 gttcgtgttt tataaaaata tccctgacta acatacagta ttgaaaaatc aaccgtctgt
     5161 tcttttcata ataagtatta agctctggaa ttctgcgagc taccccacta aaaaactgtt
     5221 gcatgattgg atacatcact ggaaatatat gctgaaaatc tctgtcttct gctgattttc
     5281 caaaagcatc caccttcttt tttgtcccaa aaacccttga atacaaatgg atcattgcca
     5341 aattgacata tagataattt ttttctgaag ttgtaaaaca aatcttgaag tttttttcaa
     5401 aattttctac aatagacgat tcaatccagc cccaactttc ttgtacttta tctggagtat
     5461 ccaacaaatc ttcttccaaa taggtgttca ttacaccaaa cataaaataa aggtaagctt
     5521 gctctgttgg agtgatgtga ttcatcgacc agaagtttgc ttcaaaaaaa ggctctagta
     5581 tgtaatgcgt aaactcttct tttgttatca atggatgcac aaatttgttc atctcttctg
     5641 ggacatacgt aatcgagtgg ccttgtttca ttcgttgcaa cgaaattctt agtaggctga
     5701 tccatttctt tctatctata ctacgcgtat atggcgcatg tttcgcaaat ttttctaata
     5761 gttgacgttt attttttata cttgataata tatcagaatg attttcgtcg aatgactgcc
     5821 aatacatcaa gtgaaaaaag tatcggatac ggtattcttc tccttctatc ggattacctt
     5881 ttttcaagct gcaagataaa tgatagtttt tcaaaatagt attaagttct cttaattttc
     5941 tttgaatcgt acgcaaactt atataatttt ttgactaaga ttttctaagg attgataagt
     6001 acctttgtaa agtgaatcca gtaaaatata accaatcgtt ccctctaaaa gtatagaata
     6061 aactttactc agtaaaaact gagaagaaaa ctcgactttt atcatcttgt tttctgaaag
     6121 aaaagataat ttatattctg gatacctttc gcaaaacgat tctgcttgtt gaatcaccga
     6181 attcatctta gccagggtgt agtttagatc taaagcgata tccttcacca catataattt
     6241 ctcgtctctt tcataacaat agttcaaaaa ttgatatact tgcctttcat tgatatcaat
     6301 aaattcttca aacaaagtac atactccttt ttatctatta tttagactta ttccgacagc
     6361 tacatgaata acgtacaaat agattcgtta ctgatttttc agagatacta ccatgcgcag
     6421 tgtgcattgt caattattct tgctcttatt ttccatttgt gttcctgtta tttcctgttt
     6481 ctcaaatatg ttagtaaaca aaaatagatc tggcaaagct acaacttcgc caggcaatac
     6541 agcactctcg aatgaaatat tcactcaaaa atctatcgtt ttattcaatt ttagatctgt
     6601 tgcttttatt ctttatttca ctcgatggaa gagagaacga aaatagcttg ctcctacaat
     6661 caaaatgagt aacacaccca tccatgtaat tagcttgttc aaggttctac taccagtttc
     6721 tggtaaatcg ctttttctat tatctgtttc tttcgctttt tcttgatgat caattccacg
     6781 agaaacaaaa tcaggctgtt ttagcacact ttttaggttg ctccctgcat ttctcttttg
     6841 ttccttagtt ggtatgatag gtaccaaatc aagttcatta tttacttttc ctgtgctgct
     6901 ttcgctgctc gtgcttcctg tgctactctc actactcatg cttcctgtgc tactttcact
     6961 actcgtactc tccgtactgc tttcgctgct cgtgcttcct gtgctacttt cactactcgt
     7021 actctccgta ctgctttcgc tgctcgtgtt ttctgtgcta ctttcactgc tcgtactctc
     7081 tgtactgctt tcaccgctcg tgttttctgt actgctttca ctacttgtgc tatccgtact
     7141 gcttggcggt ccaacaactg tccaactgct tggtttcaaa ttctccgtga tcgtcatctg
     7201 tccatcataa aaaacaatca aatcaacttt atctttactc ttgatctcat caatcgatag
     7261 ttctgcagaa atcattattg tatactcaat accagcggtc cattgtacgt cttttaaata
     7321 aacttcccca ataacttttt ctgaacttcc attaccatcc caaccatatg ttagaacagc
     7381 atgtacatca ctagcattca cgcttttgtt tggtttgact ggaatagtaa tgacttgttg
     7441 tgtcatgtca taaacaatcc gatcagtttt cacagtaaat gaaagattct catttgcccg
     7501 ttcatttgca gaagtactga ttccaatcat tgctgcaatt atactcagcc caagaataac
     7561 aaataatacg gtcgctgttt tcttcatttc tatcactctc catataatta ccttctattt
     7621 gataaaggaa ttatataaaa tgaaaatgat gagaacacga cagtcagttg tgaaaatcta
     7681 acaaaagatg tcgtgtattc gcttgaattt gtttactttt tcgtttaatc aatgacagac
     7741 ttaaaaaagc aagaccgata tcagccttgc tttcttcatc ttttattgat aaacagcagt
     7801 gtatttcttc atccgctttg ttttatatgc atagagtaac aaaccaacaa tcaagactaa
     7861 caagaataat gtagttgata tagatgcttc agccccaaag tttcccccag taattattgt
     7921 attttgcgat tggatcagag tggagaaaaa accttgttct tgcgttccgc tgactggtat
     7981 acccaaaata gctccttgtg tccaattcca agtaatatga gcaccaatag ccgtaaataa
     8041 acttcctgta ctgtagataa gtgcgctgaa aaagcaacct gctaaaaaga tattgaaaac
     8101 tgccagaaat gtcatgttag gattcaatcc gtgcaaaaga gcaaataatg cagcagatcc
     8161 aatcattgct attaacggac caaatctaag catctgatgc tgaaaaaagc cacggaataa
     8221 aagctcttct gatgccccca ctgcaataaa taaaataaaa ctcgttatca gcggcacaat
     8281 gtttgcgttt cctgaatcta accgatacgc ccccgtcaaa tataatggac tagtaataag
     8341 cagaaacact ccaattccaa caacgagacc tcccgctaaa tacccccagt cttttcctcg
     8401 aacagaaaca ccataatctt ctattctttt tccttcgacc ttactagcaa gaaataaagc
     8461 tagtaaaaaa acagctaaat gtccgaccgt accggcaatt gctccaatcc attcactatt
     8521 tccaataaac atatccgcaa gtgcattatt gacaagaatt cctaaaacaa aaccaactaa
     8581 gactattaaa acagatccaa tcaaccgaat aatcctgatc atctttcatc cctcctttat
     8641 attaagttta acaacccttt tctttcttgt ctcgtgaaaa ctatgtaaat gttctccatc
     8701 atagagagaa tagattttta cgaactaaga atatctacag caaccttacg aaaatgaaag
     8761 actgtctccc taattcttct gttagactgt ttttaataag gaaaggatgg gaaatttttt
     8821 gaaaaaacaa ctactaatta tgtgcctttc agcatgtgca attttactat tctcaggttg
     8881 tagcaagtcc aatgaaacga ttgcaacgtt taaaggtgga acacttacag ttcaggattt
     8941 ttatgataac ccaaaagtta aatcaaataa tcaaacacta ttaaaaaaaa tgattgtctt
     9001 caaagcattt gatgatgtat acggaaaaga gatttcttca aaggaagtaa caaaagaata
     9061 taatgaacaa atcaaaaaac tcggtccaaa ctacaaggaa caattaaaag cagttggcca
     9121 aacagaagag acatataaga aactctttaa acagatgctg gctttccaat acggtttgaa
     9181 agccaatgta aaattaacag ataaagatct agacactgct tggaaagaat tctatccaga
     9241 agttagtaca cagatcattc tattttcgac taaagaagaa gcagataaag caaaaaaaga
     9301 agcaaatgaa ggagaaaatt tctccaagct tgttcaagct tatggaaaaa ataaaacatt
     9361 aaaggaaact gatggaaaaa tgaattttga ttcaaccaat ccagagattc ctactgaagt
     9421 aaaaaaagca gcatttaaac taaaaaacgg ggaagtttct gatatcattc ctgtaacaga
     9481 tccaacgact tatcagcaaa gctattatct agtaaaaatg gtaaaaaaac aggataaagg
     9541 ttctaataaa gacaaataca aatctgagct agaaaaaatt gctaccgaag caaaaactct
     9601 tgatacggaa ttcatggata aaacaatccg taaagtgatg aaaaaagaca atgtaactat
     9661 taaagatcct tatgtaaaaa atatattcaa ataaacaaat ttgacccaat tggggatgtg
     9721 gcaaaatttg agaagtgatt tttggaaatt ccttcttatt tttgaggcaa accacttttg
     9781 ccacaacttc atcggggcct ttttttattt gtaccaaaac aaatcactgg atttgttaca
     9841 atcatgagga tataatttaa aaaagttttt tatttctatc acatgaatca atgcgccatt
     9901 aagtagattc tttcatgctt ccctattttt tcacttgccg tatcgcttca aaaacaacta
     9961 tcataaactc aaactcgact gaaaagttat tttaaaaaag actaaaaata tcccttctgg
    10021 acttgttctt cttattctgt tatactagag aaacacgaaa gatattttta tgcgacactt
    10081 ttcatttgtt tttatgaaat ttattcaaaa ctggcgtaac ttcagagtta caaggatgta
    10141 ttagaggtta gggaatacat cgtttaaatg agaaataagt aatagttagt aatagtcacc
    10201 caaactttaa attaatgaat attgaatcgt cagatagcct cctcgagttt tcgagaaggc
    10261 tatcgtttat tttatggaga aattgattca gaagtaatca gcgacctttt ttagttcacc
    10321 aatcgtttct gcttggaaag tttgcaccca atcttcttgg aagcccctca ctgctttatc
    10381 aatcgagggc atctctaata cttgatctag tagctcttta cctaacgtta tcgcatgagc
    10441 tccagatcga atggctgtag tgacttgttc acaatttttg aaactagctg ctaatatttt
    10501 tgttggtctt tgcgtacgtt cgatctcagt ccgcaacgaa tcaatcactt tcatagcatc
    10561 aatgccttga ttttgcatcc tgttataata aggtgcaata taatctgctc cattattaat
    10621 agctaaatac ccttgtaatt ctgtgtaaat agctgtaccg gtaatcaaat aaccttcttt
    10681 tttcaacttt ttgatcgctt gaaggcctgc ttgattcacc ggcaccttga taaacgtatc
    10741 ttttcccagt tcttctatga taactcgagc atcctctatc atttcttctt ccgttcttcc
    10801 tattgtctga atatggatag gaatctgtcc gattattttt ttgatttctt tcatatgcga
    10861 gaagaagttt gacgctcctt cttttttcag aattgtagga tttgttgtta cgcctgttaa
    10921 attcaaaaac tgttgacaag aataaatact ttttgtattt gctgtatcaa gcatgatttc
    10981 catatagctt tcccccattc tgatcctctt gatcgatttt aatgattgtc ccctttctta
    11041 aatgcggcat ttccttatct tctacataca ttgttcctgg aagttccggc tcatgagatc
    11101 catcaaattt tatcgtgata tgtcctaatt cattcaaatt agtttggaca acatctccta
    11161 ctgctgtgat tacatacatt tgtttatcaa aacagagctt catatcacaa gttatttctt
    11221 ttcttacctg attcaattca atcttataac aataatctgt taatgtttcc ggtgcttcat
    11281 ctccaaataa aatcattatt ttttctttcc tgaatacttc tgcttctata ccaacatctg
    11341 ttacaaccgt ttcaaagatc gtcatctct
//
LOCUS       DAJSKS010000088        11337 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJSKS010000088 DAJSKS010000000
VERSION     DAJSKS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.090"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(364..1482)
                     /locus_tag="ON396_001926"
     CDS             complement(364..1482)
                     /locus_tag="ON396_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114004.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1953..3260
                     /locus_tag="ON396_001927"
     CDS             1953..3260
                     /locus_tag="ON396_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCR3114005.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3379..4578
                     /locus_tag="ON396_001928"
     CDS             3379..4578
                     /locus_tag="ON396_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR3114006.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4605..5873)
                     /locus_tag="ON396_001929"
     CDS             complement(4605..5873)
                     /locus_tag="ON396_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HCR3114007.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6036..6682)
                     /locus_tag="ON396_001930"
                     /pseudo
     CDS             complement(6036..6682)
                     /locus_tag="ON396_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7090..7593)
                     /locus_tag="ON396_001931"
     CDS             complement(7090..7593)
                     /locus_tag="ON396_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR3114008.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7716..8480)
                     /locus_tag="ON396_001932"
     CDS             complement(7716..8480)
                     /locus_tag="ON396_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCR3114009.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8588..8863
                     /locus_tag="ON396_001933"
     CDS             8588..8863
                     /locus_tag="ON396_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR3114010.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9301..10251
                     /gene="yidC"
                     /locus_tag="ON396_001934"
     CDS             9301..10251
                     /gene="yidC"
                     /locus_tag="ON396_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR3114011.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10430..10630)
                     /locus_tag="ON396_001935"
     CDS             complement(10430..10630)
                     /locus_tag="ON396_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114012.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT         3401 a         2387 c         2002 g         3547 t
ORIGIN      
        1 taaacggtgt tccagaagta gcttcttcgg aaataagccg aaatttcaca aaaatttgag
       61 aaataatttt tgtcaatctc ttcttatttc ttgaagctga ccacttctgt cacaacctct
      121 tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaccaaa caaaaaatat
      181 gaagagctat ttttgcttgc tgttcttatt acttgatgca gaacagcttt ttcacaacct
      241 ctcatacacg gtgtttaatc agctgacctt cggtattagc tcaaaaaacg gaaaaatatg
      301 tgaaactatt tttccgtttg tttctctaat actcaggtca aaacactgat ttcacaacct
      361 cttttaataa cgaagaatcg ctgtcagact cttttcatct ggcgcatctt tttgatccaa
      421 aataaacact ttcccgccat taatgatcac atcatctgct aatgcattca gcacttgacg
      481 ccgatcatat tctgtttcag gatcttcacc gaaaccatca gctaagtttg acgtagcgat
      541 aaataaatgt gacacttttc cttctttcgc agctggaacg atatcaacta actgatcgat
      601 gaatttctta tctaagagtt tattgtattc ttttgattct tgagctgcga gttcacttga
      661 cagcttcttc gccccttctt cgatatctct aaaggatagc tgcgcaggag aagcagaaat
      721 cgcaacgtct gcacaataat agggattttt agcaattttt ttgaacatcg tctgattttc
      781 tggtaatgca aataaataaa tcggccattg ttcaggattt tcgaaagaat ccttcaagta
      841 cgtatcaaca gcttggtaat agttgaccca atcgatctct acttcttcat cttttggatt
      901 tacgccatgg aaagttactc catctcgggc tccgcctcct ccattagaag agaagttcaa
      961 accgccacct gtcagttcag atcccaatgc agtaacgaca tcaaccggtg ccccttctgg
     1021 tagagatact tcttttactt gtttgttgct gacctcatac aatttcatgg agtcgcggtt
     1081 caatcccaat aaataataag aatagttgaa ctgcccattt ttgataatag ccagtaaata
     1141 aggaagatca cttacatagt attgatcgtc taccgggata ctcaaacgat ggatataggt
     1201 ctggtcagca gttataatga ccgctacact cgtagttgca ctacgccaaa acgaaggatc
     1261 tcctaacaaa ttttcaattt tttcttggaa cggggaccag tcatgttcgg aatatttttt
     1321 ctcaaaacgt ttctttgctt ctttcgcaaa atttttgaat ttgatctgat ctttttccac
     1381 atcttgatgg gcgacatgcg tattcagcat gatcgtgacg aatggtcctt tagcgccttc
     1441 tgaagtcaat tgtgtcaata attctttatc cttttttgcc ataaacgtac ccctcctaaa
     1501 aaaatcattc ctttgtttct atctaaagtc taccacaaca ctatttttcc atacaataaa
     1561 acgtatctaa aaaaataaaa cggtttctac ggttttaaat ttagctattt ttttaagtta
     1621 tcttcataat ttcctcaatt atttagcccc agtcttcaac tattcttcat gaaatgctga
     1681 taaactaaga aaaagaataa aagaggtgga tcttatgcat ttgattatga ttttcagttt
     1741 attaagtatt atcagtttat ttgctttcag cgcttcccgc ttattagatg cggaaacaca
     1801 gcctattcct gtaaaagtac gtaaaaacga ccgctaaact tccataacgc gcgttcttat
     1861 cctaaagttt tttcagaaac attgaaactc tctcccctct ctgataaaat tttaatttgt
     1921 agcgaaacaa attaattaca aggagatcgg atatgtccat aaaaaaaaca cgtctagtcc
     1981 tattattggc tagtctcatt ctttgccttg cttcttttct accagctgtt gctgtgcaag
     2041 ctgaagatac gttcaaagta aatgcaaaag cagcttttgc tgtagatgcg caaagcggta
     2101 agattcttta tgatcaagat ggagaaaaaa ccatggggat cgcttcaatc acaaaaatca
     2161 tcggtcttta catcgtcctt gatcaagtaa aagaaggaaa actgtcatgg ggtgataaag
     2221 tttcgatttc agattatgca gaaaatttga gtatcacccc agatttatcg aatgttcctt
     2281 tgcataaaga aaacacctac actgtcaaag aattattcga ctctgcaatc atccagtcag
     2341 ccaatgcttc aatggtggcc ttggcagaaa aaatctctgg cagcgaagct aaattcactg
     2401 aacgaatgaa agaacaattg aaagattggg gaatcaaaga tgcaacaatc gttaatgcat
     2461 caggattgaa caactcttat cttggcgaaa accgaccaga aggcactgga gaaaacgacg
     2521 aaaatcaaat gtctgctcaa gatgtggcca tcgttgcccg ccatttgatc ttggatttcc
     2581 cagaaatact ggatgtatca agtactacga cccaaatgtt tggcgaaaat acgcaatcac
     2641 cagtggaaat ggtcaactgg aactggatgc tcccaggatt cgttaattac aaggaaggcg
     2701 tggacggatt aaaaactggt acgactgagc ttgcaggtgc ttgtttcgtt ggtacgatca
     2761 ctaaaaacga tcaacggatc atcactgtcg tattgaatgc ggacggccat gcggaaaacc
     2821 cgagtgcccg cttcaatgaa acagaccgtt tgatggacta ctgctatgac aattggtcag
     2881 aaaaagagct cggaaaagct aacgcaagta ttcctagtct aaaaaccatt gatgtaaaag
     2941 acggaaaaga atcaacagtt cctgttgtgc ttaaaagccc tgtaaaagta tgggtacgaa
     3001 acgatatgga tactggcaaa ttaacgatca cgccaacact tgacaagaag caagtcactg
     3061 acaatgagat ccaagcacct atggataaag gtacaaaaat cggtaccgct acgatcacct
     3121 tggcagacga taaattaggc tatttagaag acaacaatgc accatcaaca gagatcatta
     3181 cggataaaac agtggaaaaa gcgaatatat tcgtgattgg ctggcgacgc gtcgcagaat
     3241 ttttcggcaa cttgttttaa ttgaacaata agaaaaacat ctttccatcg gaaagatgtt
     3301 tttttgcttt ttctagttta aataacagct tttttcatct attttcgtta tactgtataa
     3361 gaaaaaggag ttgaatttat gaatctaacc agagatctga tctttttagt tctctatctt
     3421 attggggtat tctctatcgt ttattggcga aaacagaaga aggatccttc cttgttcttt
     3481 tacggcagta cgtggacatt tacggtaatc ttaacttatt ttgtcgtgtt ggaaattttt
     3541 tattcgacct tttatttttt gattccattg ttgttcttta gtatctttgc tttttcttat
     3601 ttcacagaaa aacgaaggct actgaatggt cttttattca atgttttact catcagtttc
     3661 ggcatctact tgtttgtctt gctgtatgaa acgcaaaata tttttcttgg cggactgatc
     3721 gccttgatca cgattccatt gctacttgtc cttttatttg gtatttatgg attgattgtt
     3781 tttctttttt ggaacggggt cacagtttta cgtagagaat cgcattcatt agctaatctt
     3841 ttaacgttga tactagccat ttttcttaca ctgtttcttg tttttgactt tttcttgtta
     3901 aaatatttgc cgcaatggat aaatgctttg tttttctgcg ttcccctgat cttgatctat
     3961 ctatttatcg tattctacaa ctttttgact gtctcattct tgtatcagtt caaccggcct
     4021 cgatacaatc aagacttcat cgttgtgtta ggcgctggat tgatcaatgg agaaacagtt
     4081 tctcctcttc tagccaagcg aatcaataag gcaattgctt tttatcgggc tcaaagccgt
     4141 gctacattaa atccgcccat cttattgatg tctggcgggc aaggagcaga cgaaaaagta
     4201 ccagaggcca ttgcgatgaa acaatatgcc atggaacaag gaatccctga aagagacatt
     4261 cttgtcgaaa ccaattcgac aaccactttg gaaaacatgc tctattcaaa ggaaatcatg
     4321 gatcagcaaa tgaaagggcc ataccgcgcg atctttagtt ccaacaatta ccacattttt
     4381 agagccggtc tttatgcgcg gcaagccaaa ttgaaagcaa atggtattgg tgcaaaaaca
     4441 gctttctact acttgcctaa tgcgttttta agagaatacc tcgccatctt gatgatgaac
     4501 aaaaaaagac atatgatcat ttgcggattg cttttcgcag gctccgcatt cttatcgctg
     4561 attactttac ttttctaaaa aaacaaggtg tgacaaaaca tatgtcacac cttgtttttt
     4621 tctttcattc tcttaactat tggcaaaaag agtatccctt ttttgaccgc tacttgctga
     4681 gcagaataaa taccgctgtt gcctgaacac ataaagctga tcaagcagat cataaagaag
     4741 tatactgccg cttgtgaacc gaacaattcg atccccatca caaagcaggc aatcggtgta
     4801 tttgtcgcac cagaaaatac tccgataaag cccaatcctg caagaaaagg aacagaaaca
     4861 tggagcaaag gcgctagtgc tgcaccaagc gttgcaccaa tttcaaacaa tggtgtcact
     4921 tctccccctt gatatcccgc gcctaaagac aaaactgtga agaataactt cccaataaaa
     4981 tcatagacat gtgcctgtcc attaaatgat tcttgaagta acggaagact caatccgaga
     5041 taacgttgtg tttgcaaaat caatgctgct gccacaacta cagctccacc tatcgtattt
     5101 cgacaaacag gatgaggaat ccattgtgca tatcgcgctt ttaaaaagac gatcgaacgg
     5161 ctgaataccc aaccaatcaa accaaaagca atcccagcaa caaaaacttt gaagaacagc
     5221 aacatactcc attcaggaat cacacccatt tggtaatgta gatgagaaac accatagctt
     5281 tctgtcatga aatttgcaaa cagtgcagca aagaagctag ggaagatcgc ttctgttcgg
     5341 actttcccga ttgccaacac ttctaatcca aataatgttc cagctaaagg tgtgccaaat
     5401 acagaactaa aacccgcact gattccactg ataatcaaga tttcccgttc tgatttatcc
     5461 aatcggaaca aacgtgccgt attatctgct aacgctcccc ccatttggac tgctgttcct
     5521 tcacggccaa cagatccccc aaataaatgg gtagttatgg ttccaaacag tgtaagtgga
     5581 ataagccgca acgggatttt ttcttctcca ccatttcctt gatcgatcac taagttgttt
     5641 ccacgactag aaagtccgcc gtgatacgca tagagataag caaaaagcgc accactaact
     5701 ggtagtagac aaaggagcca aggatgtgat agtcggatat ctgtcacaac tgtcaggcta
     5761 tttaagaaaa aagcagacaa gctccccatg aatataccga gaatcgctgt gatcaccagc
     5821 cacttcagca tatacactaa aatgcgtact ggactcggta attgtttttc cataaaaaat
     5881 tcctcctcaa taaaaaaacg cctactaaag aaaggggctg tccccagact ttagtaggcg
     5941 tcattagcta tctttcgtta gcatttaaag gcgaacacca tcacctattc acttccatta
     6001 aaagatagca tatttttctg taaaaaacaa taaaattatc gttctttctt tacacgttca
     6061 gcaaattcat agaaaggagc caatttcgtt aactgttgat tacattggtc actatcatga
     6121 caaatataat tccccttttt cgtatacgtg ccatcagaac ctgctttcgt tgtagaaaga
     6181 aaaagcgaaa cattcgatgt tttctgacag attgcacaga ttcccttatt tatcgttggt
     6241 gataaagtac cgctaatccc ttttaatttt tcatcttcgt aatagaccat gaattttttc
     6301 tgcgtacctg gatcattcca gccgatgtac gtatgttctt gcaagtcgat ttcattccaa
     6361 gctggttgtt tgagtttttt cacttttcgg aataatttct cgatttgtgg tttgctaggc
     6421 tgcataaacg gctgaacgat ttgcttcagc tcctccaatg ctctttctgc ttgcgctttt
     6481 gtcagtttta cgtctaaaac tgtcgttaaa aatgtatcga ttctggcagt tcgtgatcta
     6541 atatgtctcg aatcttttca gctgctagtg cctgaactgt ttgtaccgtt tttggatcat
     6601 tgacagatga gtacgcattt aataactgtt ctacttctct tattataaaa aaatattggt
     6661 atggttttat tgtttgttcc atgattttaa agtcctttct tctaaccaaa gtgcatactt
     6721 tgtagaaaaa agtgatcttc tgcaaagtat gtgttacctg aagatcccgg actcttttaa
     6781 ataagatacg ttttgcatct tatcatcacc ccctttaatg cttattattc tatgtacatg
     6841 attcattcga taaataaaag taaaccgtat cttgaacaga tagtcaactg caaacagaaa
     6901 aaaggactgt gacaaaagtc gtgtcaaagt cccttgtttc gaatgaatgg tgttcaaaag
     6961 ctggcatctg cggtaataag ccgaaacttc acaaaaattt gagaagcaat tttcgtaaat
     7021 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tcaatatggt ctattattgg
     7081 ttttatctat taaatcgagg attctttccc aaagtaacgt tgccatctct tacgcatact
     7141 tgctgacata ggctgagcgg cttctttgac tgcgcagtat ttgtctacga atgttacgat
     7201 atatccttct ttgtacttag gcggtgcaat cgttgcgccc cacatgtgct tcaaaatgat
     7261 atctcgctca agatccgaca attcggtgat cttttctgca ttctttacag caattcttgg
     7321 atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta cgccaatcat agtagaacag
     7381 atcatgcaaa agtccagcac gcgctgttgc tttagcattg ccaccccatt tttttgcgag
     7441 cagataacta tagtaagaaa cactgatgga atgctccaaa cgagtggaat gaacatgctg
     7501 tgtgtaattc gctaattttt tcactgcttc tgtttctaaa agatcttcta cataagacat
     7561 atattcttcg tcttcacgcc atttttctgt cataattatc gatctgccac ctttttgaat
     7621 ttcattttct tctgaaattg atctaagtat accaccagtg ttcggattat tctagtcgcc
     7681 ttggataaaa tagccaaagc ttaatagttc cttaatcata aagaaaaatg ataaagaaca
     7741 atccctgccg caaccccaac attcaacgat tccgcatttc ctttcatcgg gatatatacg
     7801 ttctgatcag tcaaagacaa gatttcctga cttaccccct gaccttcgtt ccccatgatt
     7861 attgcgaagt tatctgattt ttctactttg tctaatgcgt gtgcttcttc atttaattct
     7921 gtgccataca ctgtaaaccc tttgtctttc attaaagaaa tcacttctgt caaatctttt
     7981 tgcaacacag gcaaatgata attgcttccc tgcatgctgc gcagcacctt tgtactataa
     8041 atatctgccg ttcctttgcc taataaaaca ccagccatat caaatgcatc tgccgtacgg
     8101 atcatcgtcc ctacatttcc aggatcttgt acattatcta acaacagcca tcctcctgaa
     8161 aaatctggcg tgccttcagc cgttaacgcc atcactgcta agattccttg tggtgtaggt
     8221 agatccgata cagcatccat gacctcatcc gaaactagaa aagtagaaat ctctttgttt
     8281 gcggccagcc agtctcccca ttctttcatg cctcgctgtg tcacaaaaag ttcttttatt
     8341 tctgcatcag acgacgcagc ttcttcaatc agatggaacc cttccaaaag atattcttcc
     8401 ttttcttccc gatatttctt tttatgtagt ttttttaatt ctttgattct tggatttttt
     8461 gctgattgga tctctttcac attcctcacc tccccatgat tatatcatgg aataggtgct
     8521 tgagcttgtt tcatcctaaa aaaatcggta tcattaattt ataaaatagg aaagaaaggt
     8581 gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc gtccaaggcg taggatttcg
     8641 ctacatgacc aagatggtcg ctgatcaact aggcgtgact ggaacggtaa aaaacgaaga
     8701 tgacggctca gtcacgatcg aagcaatcgg caatgacgac atcatccaga aatttattga
     8761 agaagtcaag aaatctccga gcccaagtgg acgagtgcag tatgtggatg tccaagaaga
     8821 cccgatgatt gaggaacgaa agaaatttga tgtggtggga taaggttgtg aaaaagctgg
     8881 tctgcatcaa gcattaagga caggttgtga aaaatagctc tccatatttt tcaacaggct
     8941 ggacttaatg ccgtagatgc aagcttttga acaccgtcga taaggttatt cttgaggcgc
     9001 tttgcctcaa aaattaagga cagcagtgaa aaagctgatc tatcacttgc agtgagttca
     9061 tttgactccg ctttttcggg cagaaaaaaa gtttttagtt tattaaagta agaaagaaac
     9121 acttggaatc attctacttt ttcctagtat ttctttcttg attttttgtc tttttttcaa
     9181 aatttttcgc tacgtaaaag aacgaatatt tatatgaaat ttctatgaag ttgattgaat
     9241 tccatgcgct tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct
     9301 atgaataaat ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta
     9361 tctggctgtg tgcggatgga ttcaaacgga aaccctgaca cttcgggaat cgtttatcga
     9421 gtactcgtcc atcctatggg ccaagcgatt acatatctag ttgaaaattt caattggtct
     9481 tatggctggg cagttattgc aatgactgtg atcgtccgta tcatcatctt gccactaggt
     9541 attagtcaat cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa
     9601 gttgaagcag cccaacaaaa attgaaagct gcaacaacac gtgaagaaca aatggcagca
     9661 caagcagaaa tgcagcaagt ataccgcgaa aacggattga gtatgaccgg cggaatcggc
     9721 tgtctgcctc tattgatcca aatgccgatc ttctctgcct tgtactttac ggcacgttat
     9781 acagaaggta tccgggaatc atctttctac ggcatcgatt taggctcacc aagtatggta
     9841 cttgttgcca ttgcaggggt cgcttatctg cttcaaggat acatctcaac gatcggtatt
     9901 cctgaagaac agaaaaagac gatgcgtacg atgctgatcg tctctcctgc tatgatcgtc
     9961 ttcatgtcga tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc
    10021 agctgtctgc aaacattcat cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg
    10081 gcagaagagt tgaaacaaaa cccaccaaaa caagtcgtca ctccaagaat tgatgtgaca
    10141 gaatcagcta aaccagctga cagcaaacaa gcaaccaaac aaatcaagca acccaaaaac
    10201 agtacaaaca ataaaggccg caacgcagga aagcagcaga agcggaagta gatagtaaat
    10261 aagagtgtaa taaaaattca ttaacagttt tagcaattaa aaatatcgac caagattttt
    10321 gaaaaagaaa tctggtcgat atttttattt aagtgaatgt ttaatttgaa tcatcgattg
    10381 atcgctatct aattgcagca gcatcttttt atgaagaaac aactttttct tatctatcaa
    10441 cgtagacagg tgttgctgtt tcttctaata gtgtacttaa ttccttaata ttttgttttc
    10501 cttcttctaa catccaacta ctcgcttttt cttcgccttc ggtaacaaaa ggcgccactc
    10561 ccttcgccca tgttgctcgc ccacataaga caccattctc cataaaggac aacgcggaga
    10621 taccacccat ctaaataaca tccataaacc tttctagtag aagaatagtg atagtatcca
    10681 tccctttttc tactgggaat acaattacac gattcaaagc ttgatacaca acatttccct
    10741 tcttataaac cgctattgta accgaattgt tttgaatcac ctttttggga tagaaactgg
    10801 ctcctttccg caacttctta aatgttggtt tcttttatac tttctttgta gcaactacaa
    10861 atttcttcaa atctctaaat gtttgcataa caaccgcctt tctgactttt actcaggatg
    10921 gtatttaaaa atcagcaaaa taaggcttct ctccatcttt tttatttcct agctgcttta
    10981 aagacactct tgctgttttt taatctctat caagggggct ctgaatctat tttgatgaac
    11041 ttatcttatt aatttctcaa atgagctgtc agacacaact gtattttttc aatctcatac
    11101 cggacaacta tgccagctta aaaaaatttc ctctggcaaa acttctttat tgaaaaatga
    11161 caatgaaaaa agaccattct ttttgttgaa tgctcattgt gtaaacatat ttcctgcgac
    11221 tgctcttcga accattctca gtgcaatccc taatttatct attaaatctt cttttagttc
    11281 ctcatctttc atattcaaaa tttcctttgt atgttacaca agtttgttct ctaaata
//
LOCUS       DAJSKS010000089        11246 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJSKS010000089 DAJSKS010000000
VERSION     DAJSKS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.091"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(24..959)
                     /gene="murB"
                     /locus_tag="ON396_001936"
     CDS             complement(24..959)
                     /gene="murB"
                     /locus_tag="ON396_001936"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR3114013.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1032..1952)
                     /locus_tag="ON396_001937"
     CDS             complement(1032..1952)
                     /locus_tag="ON396_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3114014.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2065..2817
                     /gene="xth"
                     /locus_tag="ON396_001938"
     CDS             2065..2817
                     /gene="xth"
                     /locus_tag="ON396_001938"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR3114015.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3681..4214
                     /locus_tag="ON396_001939"
     CDS             3681..4214
                     /locus_tag="ON396_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCR3114016.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4704..5228)
                     /locus_tag="ON396_001940"
     CDS             complement(4704..5228)
                     /locus_tag="ON396_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3114017.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5221..5694)
                     /gene="tsaE"
                     /locus_tag="ON396_001941"
     CDS             complement(5221..5694)
                     /gene="tsaE"
                     /locus_tag="ON396_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR3114018.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5817..6797)
                     /gene="pta"
                     /locus_tag="ON396_001942"
     CDS             complement(5817..6797)
                     /gene="pta"
                     /locus_tag="ON396_001942"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR3114019.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6950..7630)
                     /locus_tag="ON396_001943"
     CDS             complement(6950..7630)
                     /locus_tag="ON396_001943"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR3114020.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7756..8625
                     /locus_tag="ON396_001944"
     CDS             7756..8625
                     /locus_tag="ON396_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3114021.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8651..9166
                     /locus_tag="ON396_001945"
     CDS             8651..9166
                     /locus_tag="ON396_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114022.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9213..9806)
                     /locus_tag="ON396_001946"
     CDS             complement(9213..9806)
                     /locus_tag="ON396_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3114023.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9831..10010)
                     /locus_tag="ON396_001947"
     CDS             complement(9831..10010)
                     /locus_tag="ON396_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114024.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10087..10596)
                     /locus_tag="ON396_001948"
     CDS             complement(10087..10596)
                     /locus_tag="ON396_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114025.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10692..11072)
                     /locus_tag="ON396_001949"
     CDS             complement(10692..11072)
                     /locus_tag="ON396_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3114026.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
BASE COUNT         3230 a         2301 c         1996 g         3719 t
ORIGIN      
        1 cttattttaa gttccattac ttattaattg gctaaacttc tgcttgtttc tgatattgct
       61 tgtttttctc caatgatccg cacttctgtt tctaaagaca catcaaattt ttctttgatt
      121 acttcttgga tgtgcgcaat caattcaata taatctgtcg ctgtcgcatg atcgatattg
      181 acgatgaatc cagcatgttt ttcagagact tgtgcaccgc cccatttcag gccttgcaat
      241 cctgcttgtt ggatcagctg acctgtatag tgtcctactg ggcgcttaaa gacacttccg
      301 catgaagggt attctaatgg ctgttttgat tgacgcaatt cggtcaactc atccattctt
      361 gctttgattg cttcataatc ccctgtttgc aaactgaatc gagcttccaa tacgatagca
      421 cgtaattctt ggactttact gtgacgataa gaaaatacca tttcgtcttt tgtcaaagtt
      481 ttcaatgttc catcttcaag caaaagatcc acttcagcaa aaacatcttt gatctctccg
      541 ccatacgcac ctgcattcat ataaaccgct ccaccgacac ttcccgggat gccgcaagca
      601 aattcaaatc cagtcaatga ttctgccaaa gctgcataag tcgtatcaat cagtgaggca
      661 cctgcttccg caatgaccat cgttcctttg acatgaatct gtttcatttc agtcagcatg
      721 atgactacac cgcgaatccc gccatcttga acgatcagat tgcttgcatt tcctaatacc
      781 atccaaggta tatcatggat tcggcaataa tcgatgattt gctttacttc ttcttgtttt
      841 ttcgggaatg caaggacatc agctggtcca cctgttttcg taaacgtata gttcatcaat
      901 ggttcatcaa acaatatagt aatcgtggct aagctttcaa ctatttcttc tttattcact
      961 gaggattttc ctttcttttc tcaaacgtcc tcattattct atcacgctta atacaatctt
     1021 gccagtcttg ttcaaagttg atgacggtag tgatctttaa gcacttcctc acataattca
     1081 tgacttctta caacttcttt ttgtttaagc aattctgttt ccgatttacc taaacggatc
     1141 tgagaaaaga aggcctctac cattggctca aacccgcgtt tgactagcgt gtttgtccag
     1201 tcgccaaatt ctttcacttt ttttccatca gcatcttgaa tgacaagctg ggtcagatcg
     1261 tttacttcat aagtaccatt tttacttgtt acttgatatt tttctatatt agcgccactt
     1321 gctaaatcca tcgttaaaat agccgtttga ttttgtgttt ctaattgtaa aatggcatat
     1381 tccaaaaatt cctttgtttc tcttatctgg tattttgttc tcaaaatggg ttcatctaat
     1441 aaatacacag ctgtatcaat aagatgtaaa aacaaatcat aaaggacgaa ctctgtcgtt
     1501 tcttttgccg ctattcgatt tttttgcagg tggatcaacc gcttatctgg gatacgtttc
     1561 aattcttcta ccataggagc aaagcgccgg ttgaaaccga tcatcaaaat tatttggttc
     1621 ttatcagcta attcttgcaa ttcacgaact tcatttagat ggacactcag tggtttgtca
     1681 atataaacat ggataccatt ttctagacat tttttggcta attcataatg tacttttgtt
     1741 gctgcgtgga tcatacatgc ttcgattttt tcttctaaaa gctcatcgat cgtttcatat
     1801 agatgttgga acccgtactc tttttgaatt ctttcttttg tttctttatt tcttgttgca
     1861 aaataaaaat caagtgttcc ttgttttttc gcataagttg gtaaatatgc tttttctgcg
     1921 atattcccta caccgattac accaatcttc atctactttc ctcctggatc atttagttac
     1981 tttcatctta caccattttt tactaaaatg aggtacaata gtatcgcaaa ggtaaggaaa
     2041 ttaattcgaa ggagtgtgct tttattgaaa tttatctctt ggaacgtaaa cgggcttcgt
     2101 gccatcgtaa ataaaaattt tttagaagtt tttcatgagt tagacgccga ttttttctgt
     2161 ctgcaggaaa cgaaactcca agcaggacaa attgatttag atcttccagg ttattatcaa
     2221 tattggaatt acgcggaacg aaaaggctat tccggcacag cgatttttgc taaaaaacca
     2281 gcattgaatg ccacttacgg aatgggtatc gatatccatg atacagaagg ccgtctgatt
     2341 actttggaat attccgattt ctttcttgta acctgttaca cgccaaattc tcaaagcgaa
     2401 ttaaaacgtt tagattaccg attagaatgg gaagaagcct tttataatta tttggaaaat
     2461 ctaaaaaaac aaaaaccagt catcgtgtgt ggggatctaa atgtcgctca tcaaaagatt
     2521 gatttgaaaa actggaaaac gaatcaaaaa aatgctggat tcactccaga agaaagagcg
     2581 gctctttctc gactgttgga taacgggttt atcgatacat tccgttattt ttatccaacc
     2641 caggaaggtg tctactcttg gtggaactac cgattcaatt caaggaaaaa caatgctggt
     2701 tggcggatcg attacttttt gacgagcaaa gatttagagc caggactagc tgatgcaaag
     2761 atccatacgg acattatggg cagtgatcat tgtccagtag aattagattt gaaataaaga
     2821 ggttgtgaaa aagtcgttta gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc
     2881 acattttgca taaatttggc ataataccga gaaactggct tttgaacact gtttatcgag
     2941 agattgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
     3001 catatttttc gtttggttgg gcttattacc gcagatacaa gcttttgaac accgtttatt
     3061 aagaggttgt gaaaaagctg cactgcatca agtaataaga acagccaaac aaaaatagtt
     3121 cctcatattt ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt
     3181 attaagaggt tgtgaaaaag ctgcactgca tcaagtaata agaacagcca aacaaaaata
     3241 gttcctcata tttttcgttt ggttggactt attaccgcag atgcaagctt ttgaacaccg
     3301 tttattgaga agttgtgaaa tcagcgtcct gacctgagta ttagagaaac aaatggcaaa
     3361 atagttcctc atattttgct attttttgag ctaataccga gggtcagctg attgaacacc
     3421 gtttagtgga gaagttgtga aaaagtcgcc tactttcgag tcgtaagaaa tttttttgaa
     3481 aatggttcct cacaactttg cataaatttg acttaatacc gagaagtcat gatccccatc
     3541 taactccaaa tcacaaggaa tgagttcaaa aactagcttt tctcaacaaa tattacataa
     3601 aaaattctat gttttttgtt aaatttattt gcttttttca aaagtatgtt acagtaattt
     3661 cccaaggagg ttttacagaa atgaattttg ttgccatgga ttttgaaact gccaatcatc
     3721 agccttatag tgcttgttct cttgcgctag tcatggtaaa aaatagccag atcgttgatg
     3781 aattctatac attgatacag cctgagactc cttttttctg gcgaaatgtc caaattcacg
     3841 gaatccatca agaagacgta cgcaatgcgc cgaaattccc agaagtctgg caaactatcc
     3901 aaccatattt tcaaaaaaat cgtttagtcg ttgcacataa tgctgctttt gatacaaagg
     3961 tcttagcggg atgcttagat tattatcatc ttgaacagcc taattatctt tctttatgta
     4021 cagtgaaaag tagccgtcgc ctgtttcctg aatttccaaa tcatcgattg aacactgtgt
     4081 gcgaaaattt gaacatccaa ttagatcatc accatgatgc attggaagat agtcgtgcat
     4141 gtgcggaaat cctgctttat caagaaaaaa aattcgggac agatccattg aaaaaacttg
     4201 tgactataaa gtaaaagaat acttatgaaa cacacaaaga ggtcgtgagc ctgccgtttc
     4261 actccgagtc acaaggagca atttcaaaaa atagcttctc atgtttatta aatattgtga
     4321 ctggtgaccg aggagctggc aggagaacac cgtttattgg agaggttgtg aaaaagctgt
     4381 tctgcatcaa gtaataagaa cagccaaaca aaaatagctt ctcatatttt tcatttggtt
     4441 ggacttatta ccgcagatgc aagcttttgg acaccgttta ccaagaggtt gtgaaatcag
     4501 cgttttgacc tgagtattag agaaacaaac ggcaaaatag ttcctcatat tttgccgttt
     4561 tttgagctaa taccgaaggt cagctgattg aacaccgttt attcaaaaaa aatggactgc
     4621 gacacgactt ttgtcacagt cccttcgtgt gtttttctgc cttctcaaaa ggatgacagt
     4681 ttctcactct tttcctattt cttctattca atcaaataac tcattttata tacatctaaa
     4741 aattcaccta gatctgttct tgcccctcta ggcatgaccg cttcgatttg gaagcccatt
     4801 tttcggtaaa ggtgtacggc tctttcatta cgaacttgaa cgtcaagctc caatcgaaaa
     4861 agtacaccca tttccttcgc ccaactgatg atttcttcta gcataagagt gcctagtccc
     4921 attccccaat attcctgcag aatactgata ccgacttctc ccacgtgaga caaacgaaat
     4981 tgactgtctg ccttgacaga tgctgttcca ataatcgtcc cttcatagat tgcaagcagc
     5041 aataaattat tattgctttc gtatatataa tccaactctt cttctaaggt ttctggtggt
     5101 aacaggagtt ctgcttcatc caaaactaaa aattctgtct ccttattaac ttgtcccatc
     5161 atcaaaagaa tgtcttttgc atctcctggc actgcttccc gcaagatgat cccaacttct
     5221 tcactcatga ttttcagccc atttttctag aattcgctgt ttgaaggttt cactttgctc
     5281 cccgtatgag attagtttca cataccgctt ttctaaatct gtatcacttt tttctaaaat
     5341 cagttccaaa taatcctccg gcagtgcttc ttctaataga tttccccatt caacaacgga
     5401 aagcccatca ccttcaaaat attcatctaa ccctaaatct gctccgctag ctgctacccg
     5461 ataaatatcc atatgataca acggaatccg accttgacta tattctctaa taatcgtgta
     5521 tgtcggactt ttgatcattt gcgttatccc taatccttgg gcaattcctt tcgtcaacgt
     5581 tgttttaccc gcacctagat caccagtcaa tatcagattg tcacctggca ttgcggaatg
     5641 accaattgcc tctgcgaatt gttctgtcat tgttaatcct gatagttcga tcatttttca
     5701 tcttctcccg ctcttctttc tttttgccag tatatcgcgt aatgaagttt ttttaaagaa
     5761 aaaatgaact agtcaagaac agcagctgct gtttttggct agttcgcgtg tatttgttaa
     5821 tcaatcaatg tttgtgctgc tgtgataatc gataatttat atacatcttc ttcatttgct
     5881 ccacgagaca aatcagaaac cggtttgttc aagccttgta gaattggtcc gattgcttca
     5941 aagttaccga aacgctgagc aattttatag ccgatatttc ctgactgcaa ttctggaaat
     6001 acaaagactg ttgcttgacc agctacttct gaatctggcg ctttcagttg tgcaactgaa
     6061 ggaacataag aagcatcaaa ttgcaattcg ccgtcgatca tgtattgtgg cgctagtttt
     6121 ttagcgattt tagttgcttc aactactttg tctacttctg gcgatttcgc tgagcctttt
     6181 gtcgagaagc tcatcattgc tacttttggt tcgatatcga acaattctgc tgtttttgca
     6241 gaatctacgg cgatttcagc caattcttgc gcagttggat tcacgttgat cgcacaatcc
     6301 gaaaagacat atttttcttg atctcttcca cgtaccatga tcatcgctcc gcttgtccga
     6361 ctgatgccag gctttgtttt gatgatctga agtgctgggc gaacagtgtc gccagtcgaa
     6421 tggatcgctc cagagaccat tccgtctgca atccccatat acgtaagcat cgtaccaaag
     6481 taattcacat cttttaaaat cttttctgct tgttctttgg atgcctttcc atttctacgt
     6541 tcaacaaaag tatccaccat ttcatcccac ttatcgtaat tatcagggct gatgatcgta
     6601 aagctagatg ttttgattcc tctggcatgc gctgcatttc ttatttcttc ttcgttccct
     6661 actaaaatgg gttccacaag ttcttctgct ttcaaccggg cagctgcgcc taaaatacga
     6721 gcatcagtag cttccgggaa aacgattttg atcccacggc gaacaatttt aaatcttaaa
     6781 ctatcaaaca attccacaag atgacctcca gacgttttta tatgtattat catacactat
     6841 tccagaaaaa aagaacagtt tattttatct gtatcagtca aaaatgctgg aaaaattaag
     6901 tttttcacat acaaagcgaa ctgcactata acaggaaagc gctgttttat tacactgttt
     6961 caggcagttg ccaatcaatc ggttcttctc ctaacgcaag gagcgcatca ttcgttttcg
     7021 agaagggttt ggaaccaaaa aagccacgac ttgcagataa cggactggga tgtggagatg
     7081 ttatgatgat atggcgactc ttatcgatca ttttgatttt ttcttgtgcc ggttttcccc
     7141 acaagataaa cacgattggt ttttcccgtt catttaattt ttcaatgatc gtgtctgtta
     7201 atctttccca acctttcccg cgatgagaat acgcttgccc ttcccgaacg gtaagaacgg
     7261 tgttgagtaa aaggactccc tgtttagccc atgacactag atttccatgt tgaacaggtg
     7321 cgattccaag atcatcataa agctctttat agatatttct taacgatggc gggatcttta
     7381 cgccaggttg cacagaaaaa gataagccat gagcttgatt ttctccatga taaggatctt
     7441 gtcctaaaat caccacttta acttcttcat aaggagtcaa ttccaatgct tcatagatat
     7501 gatacatatc tggatggatc ttttgggacg catattcttt ttttaaaaat tctcttagat
     7561 gcagataata atcttttgaa aattctgagg acaaaacttc ttgccaactg ttatgaatga
     7621 tttctttcaa aaactccacc tcctaataaa aagaataact cacattttct tcaatcacaa
     7681 gggacaatct tcaccctcag cacttgatta atatggtaaa ctaggaacaa gaagcagaaa
     7741 ggaacgataa tcattatgat caaattaatt gcatcagata tggacggcac attattagat
     7801 gcccatatga gtgtttctcc tgaaaatgcc gaggcgatac gttttgcaaa tgatgccggt
     7861 gttgagttca tggtcgctac tggtcgaaac tatcaagaag ctcgcgcagc tttagatgag
     7921 gtaggaatcg attgtgcgat gatcactttg aacggggcac aggtatttga taaagaagga
     7981 aattcattgt ttaccgtgtc gattccaaat aatcaagcaa tctctgtttt agatattcta
     8041 gatgctaacg gtatttacta tgaagtagca accaacgatg gtctttattc tgaaaatcaa
     8101 gcgaaacgaa ttgaaagttt tgcttccatg gtggctaccc atttacctca ccttacgtat
     8161 aaaatggcga ttgccatggc ctcagctaag ctggagttat tgcacatcac atacgtggac
     8221 agtattcgtg ctattttgga agaaaagaat ctggaagttt taaaaattat ttgttttcat
     8281 acagaagggc cagctgtatt aggtccagtt ggaaagcaga tttctctttt aggcgatcta
     8341 gccgttactt cctctggtca aaacaatatc gaagtgaacc ataaaaacgc tcaaaaagga
     8401 attgctgttg cccatgtcgc tcacgagcgt gggatttctt tagaagaagt catgacgatt
     8461 ggcgataact tcaatgatgt cagcatgctg caagtagcag gagtcagttt cgcaatggga
     8521 aatgcagaaa tcgaagtaaa agattatgcc aaatatacaa cggacacaaa ccttgaatct
     8581 ggcgtaggaa aagccatatt gcgcgcgatt gcagaagact tgtagtttta tctagaaaag
     8641 agggatatag atgtcagaat ttttcattca ggaaaagcag cttggacgtg caactcggac
     8701 aattgtcaaa aacgaaaaag gacattccct gtttttgatg gttggtcgct ggggaacaaa
     8761 gggcgatgca ttgtctcttt atgccatgaa tggcgatctc gttgctcaca tcaagcagat
     8821 aagcctgaca ttcggtacgc gttttgagct gtataaagag ttcgaaaagg tgggaaccat
     8881 gcagaaaata ttcaattggc ccggtgactt ttattatatt cgtagacttc actggacagt
     8941 ccaaggtgat atctataatc atcaatatca aatccatcat ttcaatcagc aaatcatgaa
     9001 aatggataaa gcaacacttt tttcaggaga ttactatgta ttggatgtct caaatgatga
     9061 agatgctcca gtttgtattt gtattgctgc ggttttagat tactggctct accgtaaaga
     9121 caaaagtcgc gataagcctg tttcttaccg acttaactta agttaaaaag atgtacctga
     9181 aatgcggaaa tacgcatttc aggtacatct ttttagatat tcttttgcat atgatcataa
     9241 agatgaccac tgatcatccg ctgaccgaca actttgaagt catggcgttc gtataatttt
     9301 ttagcctttg gatttttttc atccacactt agaccgatga cggagcgatt agctttcttc
     9361 gccaatttag gtaatgcttc taataatcgc gaaccgatgc cttgtcctct aaaatcttct
     9421 gatacactga tagagtccaa ataccattcg ttgggaaacg tttctttatc aatgaacaaa
     9481 cgtgtagacg catctagctg atatttctct aagataggta ccagtggctg atcgatgatt
     9541 gattcttctt catctgtata accgtaagca actcctgcaa ctttaccgtc tatttcttct
     9601 acgatacctc gtgaataact gtaacgataa tttggatcag tgattgcttc ttctaaaatc
     9661 tctaaagtct tttgttcccc tgtctgcttc aaaaatggca gttccatatc ttttagaatc
     9721 actaagatca atggggcgat ttctggagca tcttcttttc tcgcaaaacg aatcatagtt
     9781 tcctctcctt ttctccattc taacatgcag aaaaagaaat cgcaaaatcg ttattttaaa
     9841 aaatcttcaa tcttttcagt aatgaaataa ccatgagtaa cgattcttgc cgcttcagtt
     9901 cggcaataag ggcagacgac tggcatttct ttatcattat gttcgactac attattgact
     9961 tcttcgtatg caaatttttt tccgcaatta gggcatttca ttttttccat gttatctcaa
    10021 cttcctttca tctttactct cttagcttac tcttatattg taaaaaagag aagcaatatc
    10081 cttcttctat gcagatatcc atacctgacg gtcctgttct ccatctatta atcggacttt
    10141 ataataagga cgttcatctt tgactagaag tgtccactct ttcacttttg cttttttccc
    10201 gtagtagctg actgcttcct tcatcgctgc ttgcggttgt ttgaactctt ccgttttttc
    10261 tatatcaaaa agatgttctg cggccttttc cgtgtcattt cgtacggttg ttttcccagt
    10321 ggatggattg actgctatga cttgtccttc acaaaaaaac tggtattcat atgttgcatt
    10381 cgtaggattc tccaaatctt tttcaactgc aaaatgaata gcagatatgt tatgttcttt
    10441 tgtttcgttt tttacgattg ctgctgcttt tttcaatgag atccgatatt cctggtcact
    10501 aatatcatcc atgtgaaaag cggcattgag cagtcccgtc ataccggtaa tccctattac
    10561 gatgagggca gcaacaccaa acatcatttt tttcattctt cattctcctg tttctttttt
    10621 ttaaagaata tcaagaaatg aacgaatgaa aaatcgaatg aaacgaaaaa aaagtgacaa
    10681 ttttgttatc tttattcgtg cagttctaat ggcaatccgt ctggatcaaa gaaaaacgtc
    10741 attttttttc ctgtaaaggt atcttctcta atcggttctg attcgatccc taaggactgc
    10801 aattctgcaa ttgtttcttc aatatcgtct actttgaaag caagatgacg caagccacaa
    10861 gcttctggat agtttggtcg ttgtggcgca ttagcaattc caaaaatctc caattcagca
    10921 cttcctagtt tcagatctaa tttatagtca tctcgttctt tgcggtaatt ttctctgatt
    10981 acttcaaatc ctaatttatt aacataaaaa tcacgtgatt tctgataatc tgaaacaatg
    11041 atggctatat gatggatcgt tgtaaggttc atatgagcac tcctattctt cgtttaagct
    11101 cggatctata ttaatctcca gttccattgt aaatgtgtca gattgaaaaa aatagctgtt
    11161 tttccttttt ataagaaaaa aggcaaaaaa aacagaaagt aggtcctcca agacactctt
    11221 cctgttccca atataataaa tgaaag
//
LOCUS       DAJSKS010000090        11197 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJSKS010000090 DAJSKS010000000
VERSION     DAJSKS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.092"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            149..1552
                     /locus_tag="ON396_001950"
     CDS             149..1552
                     /locus_tag="ON396_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR3114027.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            1901..2533
                     /locus_tag="ON396_001951"
     CDS             1901..2533
                     /locus_tag="ON396_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HCR3114028.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3121..4560
                     /locus_tag="ON396_001952"
     CDS             3121..4560
                     /locus_tag="ON396_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3114029.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4855..5073
                     /locus_tag="ON396_001953"
                     /pseudo
     CDS             4855..5073
                     /locus_tag="ON396_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5322..5537
                     /locus_tag="ON396_001954"
     CDS             5322..5537
                     /locus_tag="ON396_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114030.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5754..6010)
                     /locus_tag="ON396_001955"
                     /pseudo
     CDS             complement(5754..6010)
                     /locus_tag="ON396_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6197..7108)
                     /locus_tag="ON396_001956"
     CDS             complement(6197..7108)
                     /locus_tag="ON396_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3114031.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7127..8131)
                     /locus_tag="ON396_001957"
     CDS             complement(7127..8131)
                     /locus_tag="ON396_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCR3114032.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8128..10251)
                     /locus_tag="ON396_001958"
     CDS             complement(8128..10251)
                     /locus_tag="ON396_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3114033.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10256..11176)
                     /locus_tag="ON396_001959"
     CDS             complement(10256..11176)
                     /locus_tag="ON396_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3114034.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         3471 a         2106 c         2084 g         3536 t
ORIGIN      
        1 tttaaaggca gtaacaggat taaacattga aacataatcg ttactaaatg aaatcaagaa
       61 atatactgta ttactcaatt cattgaaaat atttatcata atttttaata gaaaacacta
      121 ttaaagagaa aaaagagagg taaatcaaat gaaaaaatct ttaaaacttg catcagcagt
      181 tatattaatg tcccaactcc ttatagcaac acctttccat gtgttggcaa gcgaaaattc
      241 aacatacaaa attgcaaaag aaagaaccga gacgactgaa atgagtaatt ccgatgtaac
      301 agcagagagt agtgaacttt tgacaacttc atcaagtgaa agtagtacga cttcaaatgc
      361 cgagtcttca acaacttcat caagtgaaag tagtacgact tcaaatgccg agtcttcaac
      421 aacttcgtca agtgaaagta gtacgatttc aagtaccgaa tcttcaacaa cttcaagtag
      481 tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac catatagaat cttcaaaaaa
      541 ggaagcgcca aaaacggaag gacaaacaaa aactttttcg acaccacagc aaatagaatc
      601 aacagaatct ttccaatcac aaccaatact atctggtaat gaagaaattc attttgacaa
      661 aaatgaatct gtagaaaatt ttattatgaa aattggtgag tctgcgagaa aaattggtca
      721 gaaatatgat ttatatgctt ctgttatgat tgcacaagcg atcttagaat ctgccagtgg
      781 tcaaagccag ttagctcagg caccgaatta caatttattt gggataaaag gaacttataa
      841 tggaaacttt gtcattatgg taacaaacga agatttgggt aatggaacgc tttatacgac
      901 ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc tttgaagatt atgccaaatt
      961 attaactaag ggaattagtg gaaataaaga tttttatgca ggagcattaa aagctaatag
     1021 taaaacatat cgagaagcaa ctaaattcct tacaggacgg tatgcaacag atacacaata
     1081 ctatttgaag ctaaatgaat taattaaaac ctacgattta accaactatg ataaagaagt
     1141 taaaggtcca caaatgagta agactggata ccaagtgcct attcataatt attcaataac
     1201 cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga cttgatatgg cagctacaca
     1261 gggtgagccg attcatgcag ttaaagctgg taaggttgtt aaagcagagt ttcactactc
     1321 ttggggaaat tacgtggtga tacaacacga agatggatca accgcgttat atgctcacca
     1381 gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa ggacaaataa ttggctatgt
     1441 aggaagtact gggaatagta ctggcccaca ccttcatttg gaaatttgca aagatagtag
     1501 tctttctcag agcatgttag ttgatccgaa aaccttatta ttcggagact aaaaaaggta
     1561 atcaatctga caaagtaaca agaaaatggt tggattctta gacctttgat caggatattg
     1621 agactggaat tgatatctcg attacgatgg gtttgacaaa taaaacaagt ccattatcga
     1681 atatcgagtg tattattgcc gtctttatgc tcatattttg agcaggtttc actagatgaa
     1741 aacaatttat ctatcttgag gatttcgtgt acgtatcgtt cagtcttgta ttgtaaatta
     1801 gtcataaaat cagactaaga aaaattggaa atattttttt ctgatttatt aaaagttaat
     1861 tgaaattata tggtacttct ttcttgggag ggtaggtgca gtgaaggaaa gatatggtca
     1921 ccttactgtt gttgaggaga ttactcataa tggatataaa aaatatcgtt gcctatgtga
     1981 ctgtgggaat gaaacgattg tatatcgatc aaacttacta acaggtagaa ccaaaagttg
     2041 tggatgtgga aattataaaa ataggcataa atataaaaat ttgacaaata aaacttttgg
     2101 taagttagtt gcaatcaagc caactaaact gcgaagtgaa ggaactgttg tttgggaatg
     2161 tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag cgacttctaa aaggtgatgt
     2221 aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt gaaaataaaa aattcggatt
     2281 acttactgca aagtatcctt tggacaataa ctgttcatgg aaaacaaaat gggtttgtca
     2341 ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac ttattttttg gacatactca
     2401 aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt gttgaaggga cagtaataga
     2461 gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt gttaagggag tttatcgttc
     2521 taaagagaaa tagattgcgt atataacttt aaaaaaaaag aggtactatt taggatcgta
     2581 tgattctatt aagagtgcag ctcaagctag aaaagatgct gaaaataaat tgtttcaacc
     2641 tttgattgac aaatatcgag gtaatcatta attattgtgg ataataatta acagtgatat
     2701 ataaacatct ataatatatt attcatacta ttgatattta gtataatcct atggaattga
     2761 atatcatagc aatgtatttg aaaagcaaat tggaaaacaa ataggtgcaa caccataaat
     2821 atgaaaaaat tgggaaaaag tagaatggaa atttgcttac gtctcacgag atagctcaca
     2881 ctattaatct gaagaacaat gaagtcgact tttagaaatt gctagaagaa agtaaagcga
     2941 attatcacta gttactatag agtaagttca aataaagcat ctccaactta catggttaca
     3001 tgaggatatc aaatcgaaat tactcaagct tcctatggct agagtcgcga gaagttgttg
     3061 ggcagtagac ataggttata ttgttcttag ctacacgatc attagaaagg aaatcctatt
     3121 atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc gctggtggat tttgattgct
     3181 atatctatgt ttaccttcat gtccacattg gatggcagta ttgtcaatat tgcactacct
     3241 accatctcag atgatatggg cataccaatg aatcaagcgg aatgggtagt gtccgtttat
     3301 ctgatgattg tctgttcttg tttgttgctg tttggtaaaa ttggcgatag ccgtgggaaa
     3361 atcaaagtat ttcggattgg tacgctgatc tttgtcttgg gatcgctgtt gtgtgggttc
     3421 aatcattcgc taggtatttt attgttcgca cgggtggtac aagccattgg tgctagtatg
     3481 actatggcta caaatgccgg aatcatcacg gaagtctttc ccatacatga acgtggtcgg
     3541 gcattgggag caataggtgc gtttgtttca ttaggctcga ttgctggacc aggtcttggc
     3601 ggcttgattt tgtcccaatt ttcttgggcg tacatttttt ggatcaatgt accggtggga
     3661 atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag atatcaccat gagcggccat
     3721 acaatcgatg gagggggctt tgctgccatt gcggctacga ttatcagctt ttttggagca
     3781 atctttttgg ggcaagaaag tggctttttc catccagtcc cccttgtact gttcgtaatt
     3841 tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga aagcagcctc tccattgatt
     3901 tctttcggta tttttaagaa caagattttt accatgagct tgattacagc cttgttgatt
     3961 ttttcttcca acttctttgt caatgtagtg attccttttt atctgcaaaa tgctcgcggg
     4021 ttgagtccta gctatgctgg gctgttgatg atggtctttc ctttgatgat ggtcgtcggg
     4081 gcgcctttaa gcggctacct gaccgaccgg gttggcccgg agattttggt attagtaggc
     4141 ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt tgtcagtgga ctctcctttg
     4201 tggctataca ttgtcgctac tgccatcatg ggtgccggca atgccttgtt ccagtccccc
     4261 aataatacga tggttatgag ctcggtcact atcgaaaact tgggactggc aggctcgatg
     4321 aattcctttg cccgaaattt ggggatggtc agcgggattt cgctttctac gacggtctta
     4381 tatcaagcga tgtctgctaa gatgggccag caagtaacta actatatcgc aaatcggccg
     4441 gatgtctttt tatatggaat gaagattacc tttttaggca gctttgtttt gtgttttgtt
     4501 gcctttttcc tgactatctg gcgtatcaaa aaacaaggag gtattcagaa aaatgtctga
     4561 aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg gtgagtggga tttccatgga
     4621 tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc aagtattatc ttcaaaatac
     4681 tagtgctcaa tgagatgctt gatttgatca caagaagact gcgaaagccg ctggcaaccg
     4741 caagctaggt atcccaccga tggactacgt tttagaggct actggttatg aatacggagc
     4801 caacgctctc atctgcataa gaatacatca cccagattat gtaaagattt tcgatacttc
     4861 tgtcaaaaaa tcattaagat agataaatga tttccgtcta gtcaaattgg ttcagaatgt
     4921 agacacacag atggtaagaa gtcactcaca attcgagaat ggacttgtcc tatttgccgt
     4981 atacatcata atcgaaacgt caacgatagt atcaatattt tgaccaaggg ctatgaatac
     5041 aagcattgcc ttagaaaaca tcaagaacct taggaacaac ggggatagct tggttaataa
     5101 gagataccgc tgtttaggct tgcaagtata ctcttttatc aagaattccg tggctttagt
     5161 catgagaggc ttaatactat gaaaattttc tttagcattt ttgatttagg ttccttttct
     5221 aattataata agatgaccac tacagtaacc tactgaaata tataaacagc cactgtagta
     5281 ccattttcaa agtagtctat gagaggagaa tcttcattct tatgaacgct ggaattgcgg
     5341 atatgaattt aataaaaaaa acccttaatg attttacttc caattcaata tcaaaaggta
     5401 ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg tgaaagatca gtggaaaagc
     5461 tgaatctttt agatgctata aaaataacgg aatttgctat gaaaaatgga aaagctgaga
     5521 ttgaaatatg gagataatta tcgtctagtg aattacttgc cacttagctg aatttaacga
     5581 tccttggtgc ttgaaggggg agattcttgg gaatagagca tacttgcaag cctatgtctt
     5641 tactaacaac gatatctctt attaaccaag ctatccccgt agttctaagg cttttacttt
     5701 tatctaagca ataatcgtat tcaaaggaaa tttttgaccc ttagaaagtc tcattttatc
     5761 agacgtgagc ttaggcattg ctgaattttg ttaattcggt tttaggttgt gctttgagca
     5821 taatatgaaa gtattcttta tcacgatttc attcatctaa ggtaatgtga tgtgattctg
     5881 aaattctttc aaaggcagtt tttgcataag ctgacattac atcatcaatg acatgtttac
     5941 aatgtttcac tactaaaata agggggtaac caagaagaaa aactgaatga ttatttgtat
     6001 ctaattccat ggtatatcaa ccatttccta tatagactga ttatgacatg gattaaaata
     6061 aaaaggcact atgtcttaat tgtcggtttt cgagttaccg aatggcattc atcacccacc
     6121 tatagaggat gggcgacttc tgcctgaaat aagttacact acttataact tctaataaaa
     6181 ttagattgag tcattattat ttaacatcaa cgattttcca gtttccattt ttttgtaata
     6241 ctaatttgta ctgaaagtag ctagtggttt ttgatacatt atcaaggtat tttatctttg
     6301 tactcactag aaaatttttt ccgttttgta gataaactgg attctctaac tccgaaaact
     6361 ttaagtcaat attgattggt cgcatcgcac cattttcaac ataatactcc aattcttttt
     6421 cagaagctga tggataaagt ttaaaaaatg tttctaaaaa atcggtgatt tcttccgttg
     6481 ttttggcatc aatttctgaa tcgttttcta cttgttttgc ttcatatttt gccttgtctg
     6541 gctttccaat cattgttggc aaactagtta ctaaatgatt cctgttttca tcttcaaaga
     6601 ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt tccctctgta atatgttgct
     6661 ccactaaaaa tgaaacgaca aattcttccg agtgtttttc aacatctaga atttttacag
     6721 attgaacttc ggaactcgtg ggaatatctg ctcgaaccat atcttggatt aaagcgagac
     6781 cgttttctgt aaggtactgt tcgagattac tcattctctt ttcaattgct tctttgttat
     6841 ttttccatga aaaatattct ttcgcaaaat cctctgtaaa actttcaacg ccagaagtat
     6901 tcactacctt ctcttttata accacttttt catgagttgt atgttgatca atggcggtga
     6961 aatttttata aacgccaaaa gcaagactac aagacaataa aagccaaagg gcaagcacca
     7021 tttttcgatg cttacccacg gatacaactc gttctttttt cttttttggg ctcttttcct
     7081 tttgattccg ttcaattttt atcttcattc tttttcctct tttctcttat ttgtatcggc
     7141 cagctccgat aagatgctgc cgccaatagt tttccgttag attcgtccac cccactggat
     7201 ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc cacatgggtc acataagtac
     7261 cggcatcata ggttgaatga aagaaaacta aatcacctgg tttggcatct tttagatcaa
     7321 tatgagtcat ggcatcatat tgatcctgag ccacccgagg aagtttcagg tcaactttgg
     7381 cataacacca ttgggtcaag ccactacaat caaaccccgt ttctggactt gagccaccaa
     7441 acacataggc cgttccttca tacttaaatg cctcatcaaa aatttcttgg actattttgt
     7501 catcaaactg agttgtagtt aggtattgct tcactaataa acagtaaaac atattgccat
     7561 aattataccg ccacccacca ttttccttaa tcgcgattgg gttggggtaa tcgacttttt
     7621 gaccacctga tttttccttt gaaaaagatt cagctaaagc aaaactatgc ttcttcccgt
     7681 ttttcccgac atgatctaga tacccgccac catagttata ggcctgaata atactatctt
     7741 gatcacaccc tagttctttg gaacgcttaa ccaaagacgc aaaatagaga cagccttgtt
     7801 tgatggatgc ctcggtactt aatgaattag gtggtaatcc ggctgactca gaactttgca
     7861 taacgtcttc cattgtgcca ccagattcca cttgaataat cgccaaaaga attggaatat
     7921 actctgttat atccgattct ttggcatact tttccaccat tgcacggtgt ttcaatactt
     7981 cttctgagac actagttccg ccaggagaag tgccagaact accatttccc tcactgtctt
     8041 caccaaacaa caaacccaca cacatcaaaa tacccataaa agagatggtc agaaaagtta
     8101 atgaaataac acccactttc ttcaagttca ttttttctca cctgaccttg attttggacg
     8161 atggttcatt cttttttgta ctttttgatt ggtctgcttt ggcagagtgg acctctgttt
     8221 cacttgttgg aaagaatttt tcttacttgc tgattggctt ttcccttttg aaatgatttc
     8281 ttgactagtt agttttcgtt tgatttcggg attcagattt gctctcacaa tccgctttgg
     8341 tgcttctttt ttcagcatca tatctttctg aggttgagct gctttatcta ttggaatatt
     8401 cgcccttctt tttccctgac gatttgttgc ttcactcagt atcttgcgtt tttcgtccat
     8461 tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc actcgatccg ctttttcttg
     8521 gaacagtccg cgcttgaatt cttctggtgc ttttttcgac ttttcgattc ctttatggag
     8581 attgtatttc aaattcgttg gcgtattcct tacctgatcc tttacttgtt tagtcttatc
     8641 tttcaccaaa gcttgggtat ccaaaacttt gccgattttt ctcccagcca aattcattcg
     8701 ggattgtttc ttattatttg aaactggatt ctccttagtt aagtcagata gtatttcgtg
     8761 atcgttcggt aatcgtgaat tctttcgtag agaagaaccg gatggcttaa atttggattt
     8821 tgattgttcc tttgccaact tatttccgac tgctgcacca ccaccaaaag ccaaagctcg
     8881 tcccacattt cgattgagtt gccgcaattt tcgattcatt aacatttgcg gttttcgcat
     8941 gacgcgtctt cccattgact ggctatcatt actctggagg ttgaacatac tcataatgtc
     9001 gcccaatttg aaatagatac cggcaaaggt cacaatttgc agaaaagcaa ccatgaaaaa
     9061 tggataggtt gccgaaatat tgaaaaacat cgtggagatg ctaaatgcag tcgtaatcac
     9121 caaagtaaca cccgctcgca tcataatcgt attgaataaa cgaataatcg ccttctttcc
     9181 taaactttca taagtcggca acatggacaa taagaaacta attggcagaa acatcgcaaa
     9241 gataatgaag agaatttgcg agaacaacat aatccctgtt agaagaaaaa caaagaagga
     9301 aatgccaata ttgaacaata ccaaaaagac cacggtccct aaacggttca tggtcttggt
     9361 aatactgaga ttcgcattgt cattgtcttc aatctctgcc ttgaccgctt cttcccgatc
     9421 tttgcctttg ttttcatctg gactcacaga tagaatcttg ttgacccggt cttcaccaat
     9481 ttcttctatg ttggaatcat caaattgcag tagtaaccaa ggttgttgga cttgaatcga
     9541 aaacaaacta tcccgaatca aatccacact atcttttcct tggctttctg aattgggtac
     9601 aacgatctcg gttccaaggg taagggccgc ttcactaaca tctgagctaa agtcattgat
     9661 ttttgtgata taggtaggcg cataagcaat gaacgaaccc gatagtagaa aaatcatcac
     9721 aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt tttaataatc cggtatacgc
     9781 cacatacacc cccagagcta aaatcatcaa taatagaaac ccaaaataaa atccggaggt
     9841 ctggagtcca ttttcagtaa taccagccaa tgtttggata ttctttccaa tactttcagc
     9901 cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc acataacccg tggcgttcga
     9961 caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca taaagaccat acatcacgct
    10021 tttccctaag ccatcacccc aattccaagg caaccaatcc caagagctat ccacaaagaa
    10081 atctagttga tagttgttca gtgaatattt ggaatataga tttcctgctt caactgtatc
    10141 atcaactagc cctgtcgcct cggctaccgt accaactaaa ctcaatagaa ctatcagagt
    10201 gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt ttctttttca tcccctcact
    10261 ccattctctc ttgaacaggt ggacgggtat caaaggcatg gaagagttct tcaaagactg
    10321 gatgtacctg aaccacgcct acccgaccat acaaatcctg catcaagcat tggccatttt
    10381 ccaaatctcg taggcgtttt tgattgcttt catcttctcg atccaagcca aagaattcca
    10441 aggttttctt gatttcatga atatcggtac tgcgaaacgc aaacttcaac ccgatattat
    10501 tcttcaagcg ttcatccagt aaatcatcac tattttgcgt gacaaagtaa accccggcat
    10561 tcatagcccg cccagcacga acgagcttat tcgataatgt tttcccttgc gctacttgaa
    10621 gaaaactcca cgcctcatct aagtctacaa ttttgaagat ttctcgatta ctatggataa
    10681 agtccaaggc aaaagtggaa atgacgatta gcatggaaac actaagcaat tccatggtgg
    10741 tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa atccgctact tgaatgatat
    10801 ttaactgcct atctaaacta atcgactggg taatcgaacc atctgagaaa agcaaatggg
    10861 caaaatcata atcggtaaag gactcgatgt gatccgcgat attttccgca actggtgagc
    10921 cttcttttcg aagctcatta attacttgta ataatccccg ttgattgctt tgggtaaccg
    10981 tccgaatggc cttacgcaac actggaaact tctccccatc tcgactagaa attcctgtta
    11041 aaaaggtcag aatatcaatt gctagacttt cagaatcctt ctttttctcc ataatcacat
    11101 aaggatcaag taatccttga ttttttggct tactagttaa gttgagaata ttgatttcat
    11161 ccgcaatttc tggcaaggtt tctttccagc caccccg
//
LOCUS       DAJSKS010000091        10841 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJSKS010000091 DAJSKS010000000
VERSION     DAJSKS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.093"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            152..1123
                     /locus_tag="ON396_001960"
     CDS             152..1123
                     /locus_tag="ON396_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR3114035.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(1201..1437)
                     /locus_tag="ON396_001961"
     CDS             complement(1201..1437)
                     /locus_tag="ON396_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3114036.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(1406..1537)
                     /locus_tag="ON396_001962"
     CDS             complement(1406..1537)
                     /locus_tag="ON396_001962"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3114037.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(1613..1996)
                     /locus_tag="ON396_001963"
     CDS             complement(1613..1996)
                     /locus_tag="ON396_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR3114038.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(2029..2814)
                     /locus_tag="ON396_001964"
     CDS             complement(2029..2814)
                     /locus_tag="ON396_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HCR3114039.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            2992..4092
                     /locus_tag="ON396_001965"
     CDS             2992..4092
                     /locus_tag="ON396_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3114040.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4082..4906
                     /locus_tag="ON396_001966"
     CDS             4082..4906
                     /locus_tag="ON396_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HCR3114041.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            4903..5718
                     /locus_tag="ON396_001967"
     CDS             4903..5718
                     /locus_tag="ON396_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR3114042.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            5715..6545
                     /locus_tag="ON396_001968"
     CDS             5715..6545
                     /locus_tag="ON396_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3114043.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            6552..7583
                     /locus_tag="ON396_001969"
     CDS             6552..7583
                     /locus_tag="ON396_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3114044.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(7690..8963)
                     /locus_tag="ON396_001970"
                     /pseudo
     CDS             complement(7690..8963)
                     /locus_tag="ON396_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9240..9449
                     /locus_tag="ON396_001971"
     CDS             9240..9449
                     /locus_tag="ON396_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114045.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            9499..10215
                     /locus_tag="ON396_001972"
     CDS             9499..10215
                     /locus_tag="ON396_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCR3114046.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10117..10637)
                     /locus_tag="ON396_001973"
                     /pseudo
     CDS             complement(10117..10637)
                     /locus_tag="ON396_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3011 a         2088 c         2522 g         3220 t
ORIGIN      
        1 gaggactacc tgtattagtc ctcttttttg accagatttt gaccagtcta ttttacattc
       61 taatatattc atatgagcta attttatgat taaatggtaa gtttttcctt gtgtgtttta
      121 gtttaaacta gtgtgtaatt caaaaatcta tatggaagga acagtattaa gaatagaaca
      181 gggttctcta catgatggtg ctggtttacg gaccgttgtt tatttaaagg gctgcccatt
      241 acgctgtgcc tggtgttcga ttcccgaatc acaaagcaag cagattgaaa aaggatttgg
      301 ccagaccatg accgctgaag aggtaatgga tgagattgag aaggatgctg ttttttactt
      361 tcactcggat ggcggagtga ccattagcgg aggcgaggca ctggttcagg cggattttgc
      421 taaagaaatt ttgcaaaaat cgaaatacat tggaatcaat accgtactgg aaaccagctt
      481 ttgtggtgct tataatgaaa tccaaaaggt agctccctat gtggatacat tgtttgtgga
      541 tgtaaaaatg tttaccagca agctccacaa acaatggacc ggcttagata accaacaaat
      601 tttgaagaat attcgtcgct ttttaattga gtatcctaac tgcgaagtgc gaattcgagt
      661 tcctgtggtt ccaggtatca atatgaacct gaccgagctg ctgacgattg cttgttttgt
      721 ggcagatctt gatcggttcg taccgttaga attactaccg taccactgct atggtatgca
      781 cggttaccaa gcattgggac tggagtatcc gttggcagac acgccagcgc cttcacctaa
      841 agaaatgttt gcattggcag atcaactggc tcgcacggtt cctcatttgc tggtcacagc
      901 tttaaccaaa acatttattt atttattgaa tccaagcttt atcttatgga taaatgaaag
      961 acactcacta gctgcagcca gctcaagaat agagcttttg ctgaagtcag tgagtgtctt
     1021 tttacgatgt tttttcgaag ttaatttgtc gaatgaacac tggctcaatc ggtacatctt
     1081 cactgtttgt cggctgagct gcgatcgctg ctacgatttc taacccttgg tatacctgtc
     1141 caaaaacagt ataacgacca tccagctccg gtagtcctcc aagctccaag tatgcttgct
     1201 ttacattagc aggtcagcct gcgcctttgt gttttgcaca ataaaaaact ggttgccgtt
     1261 ggtgtggggg ccgtggtccc ctaaggccaa ggcgccatta aaatgataaa gtccgcgatg
     1321 cacttcatca gcgaaaaagc cctcatagct ggaaacagct tcatcaccag aaccatccaa
     1381 agcgccacct tgaataacaa attccttaat gacacgagcc gaaaggtgtg ttgtcataaa
     1441 cgccttgctc agctagggag cgccagttgg ctacagccag gggagcttgg tcaggaaata
     1501 agcggagacg aatttcacca gcatcggtga ccagcacaca aacttcttcc tgctgcggtt
     1561 tttctaattg aaagagagga aggtttgcaa ctgaaacgcc ttgatcctgc catcaaatat
     1621 cgaaaatccg ccactcgacc ttcgcctgat caccaaggcg agccattccc aataaaacgg
     1681 tataggcggt ttcatccgct tgaaaggtca attgcagctg tcctcgtaaa tcgttgtagt
     1741 catctggggc ggctacttga taaaaaagat gaaactgcgc atgcaaatat tccaaaggct
     1801 gatctttaaa ggctgcaaaa taagcctgaa ctttttcccg ctgcaagctg tcagccacca
     1861 aaaaattttc cagttgttgt tttggaacgg ctgctaacaa ggatcggcaa accgcctcaa
     1921 ttttcaacgc atcccaatgt cgcgcaatta aataatggat gggtgcctct tgattgtagt
     1981 cccacaggtt gctcattttt ttcaccactt tcttctatta tataggaatc acaaaatcgg
     2041 cagcggcgtc aggcggcgtt cttttccttg aatgactagc aacttttcta cctgacagtc
     2101 tgccaacagc tgactcattt ttttaaagcc cagctgataa tcttcggcaa catgagcatc
     2161 cgagcctaag gtaaagagct ttccgccgac actttgatac aaaggaacgg cataacggta
     2221 aaggtcttcg ttgccgtatt tcaagaagct cttggcattt aactccatcg ccaattctcg
     2281 ttcaacaacc tttttgaaaa tcgttagcag ctgagcctca aaatgctccg cgaattcttc
     2341 cgctgtgaag gtgaagcggc gtaatccata atcaaagtga gtcaaaatat ctccttctgg
     2401 aaaatccgtc aagacctgca acatctgatc aaaatattcc ttggtagttt gaatgggatc
     2461 tttggttaaa accacatcgt ccatataatc aaagcggcca ttttgatgaa tacttaccag
     2521 ctttaaatca taaggatgct gagcgaggta atccacgatc cgctcttctt gtcctggaac
     2581 aacgccaatc tcgatacctc gcagaaattt ggtggaatag gtttctttta aagagtccag
     2641 tttgtttata taggctggat aatccggaat gtcatctttt ccggtagatg gattgtttaa
     2701 atcaaagtga tccgtagaaa cgaaaaactc cggctgatag gctaaatagt tttcaaattt
     2761 ttcctctgaa tcaaaggaaa aatacgtgtg gagatgctga tcataatact gcatagggag
     2821 cctccttttt tctactatca taccgcaaaa gctttagaag gttaacggga attcgtcgaa
     2881 atttacaaac catatacaac ctttacattg ttcatgaaac cttttcaaga aactgattct
     2941 ctcattacaa agctttgtta gaatggaatt atcgaaaggg gagaagaatg aatgaaaatg
     3001 aatgaaaaaa ttcgtaacat gactatcggt ttattttgtg cagcaacagt aagtatcctg
     3061 ctgacaggct gcggctcagg tgaagcaacg aagcaatcgg ctgcggcaga cgactcttta
     3121 gatacgatta tagaaaaagc caaggaagaa ggagagattg ccagcttggg aatgccggat
     3181 acttgggcaa attgggtagg cacttggaac gaccttgaaa aagaatacgg cttaagtcac
     3241 gtggatacgg atatgtccag tgcagaagaa ttggctaagt ttgaatcaga aggaaaaaat
     3301 ggaaccgccg atattggtga tgtcggtatc agcttcggtc cgttggctga aagcaaagag
     3361 ttaaccttac cttataagac caactattgg gaggaaattc cggattgggc aaaggatgat
     3421 gatggggatt ggttgttaag ctatacggga accatttcct ttatcaccga taaagagaat
     3481 gtgaagcaag caccgactag ctggaaggag ctgcaagagg gtgactatca agttagcgtg
     3541 ggggatgtga cgattgccaa ccaagcacaa tttgctgtat tggctgcggc gattgccaac
     3601 ggaggtgacg aaaaggatat tcagccaggt ctgaattact acaaaaaaat ggcagaaaaa
     3661 ggccgtttgt ccaccgttga tgccagcgta gcaaatcttg aaaaaggtga aatcgatgtg
     3721 gcagttgttt gggacttcaa tggactaaat tatcgtgacc aaattgactc cgagcgtttt
     3781 gaggtaactg tgccaacaga cggctccatc attagcgggt atacaacctt aatcaataaa
     3841 aatgcccctc atcccaatgc ggcaaagcta gcacgggaat atattttatc tgatgaaggg
     3901 caaatcaatt tggcaaaggg ctatgcgcga cctattcgca aggtagagct gcctaaagaa
     3961 gtagctgaaa aattactgcc ggaggatgct tatgaagcgg cggtgccagt tgaagacaat
     4021 gaagcttgga acaagacctc tgtagagctt ccagagcttt ggcaggcgga ggtactgacc
     4081 tatgcaaagt agacttgaca aactgattgt agttatacta gacggctgtc gctacgacac
     4141 agccgtcgcc cagcttgggt tcatgaatca tttagttgag caccagcaag caagcttatt
     4201 gaaggtaaaa agtgagctgc ccagcaattc acgtccgtta tacgaagtac tgatgaccgg
     4261 cgttccagcc tatgaaaatg gcatctatag taattaccat gctcagcgat ccaaagagtg
     4321 gtcgctgttt gaattagtta aggaagctgg cggaaaaaca ggagccgccg cttaccactg
     4381 gtacagcgag ttgtacaatc aagcccccta taatcgtttg gttgatcgca tccagcatca
     4441 ggaggagcga ctgattcagc atggcatctt ttatagtgag gacacttacc ctgattcgca
     4501 tttatttgcg gatgcccatt atttagtgaa tcagtatcag ccagactttt tggtcgtaca
     4561 ttcgatgaat attgatgatg tcggtcacaa atttaccgcc gattctcatg aatatgcagc
     4621 agcggttaat caggcggaca gattattggc tatttacctt cctgaatggc tgcaaaaagg
     4681 ttatcaagtc gtggtgactg cggatcacgg gatggatgcg cacggactgc atggcggcag
     4741 cttggctgca catcgtgaag ttccttttta tttgatttca aataaacgcc aaaagctgaa
     4801 ggaacaaact gtaccacagc tgctggcggc tccattgttt tgctggttat taggaattgc
     4861 accgtcagag aaaatgcagg acattcaaga attgatgagg ggatgaaaga gatgaaaaaa
     4921 cgcttggttc tggttcctgc ctcctttatc ttctgtgcat ttttactgct gccgctgctt
     4981 tttatggttc tgtgcagctt gcacagtgat ttaactaacc agtggacatt ggccaattac
     5041 agtgatattt tcagcaacaa atattacaca caggcctttg ttaacagcgt actgattgct
     5101 gtcagctcca gtgtgattgg gttaatcggg acgtttattt tatgtttatg cttaatgaat
     5161 ctatcgacta aattgcagga gaaaatcacc tttgtttcca atttggcagc taattttgct
     5221 gggattccgc tggcattctc ttttattatt ttgttgggga atgtaggtgt gctgaaaatg
     5281 attttgccat ttctagcgga cttcaatttg tattcttggt ggggattgac catcacgtat
     5341 acctatttcc aaattccgtt aggggtcttg tttttgtatc ccagcatgaa ggaagtgaaa
     5401 aaggaatggc tggagtccgc acaattgctg ggcgcttcat cagcgtatgc ttggttcaag
     5461 gtggctttgc cttttttgcg accatcgatt gccagcacct ttgtcatctt atttgctaat
     5521 ggcatgggga cctatgaaac agcgtatgcc ctgaccagca gtaatgtaaa tcttttaacg
     5581 attcgaattg cggctttagt gtcgggagat gtgttcgcca agcctaattt aggcagtgcg
     5641 ttggcagtcg ctttaggtgt cctgctggta acggcaatgt tgattattca aaagaaaggc
     5701 aaggtggtgc agacgtgaaa aagttaataa aaccgatttt gttctgcttt gtcctgtatt
     5761 acttgctgcc gattattgga acgattttgt atgccagctc cactaaatgg tcaaaatcgc
     5821 ttttacctag tgattttacc ttgcaatggt tccaacagct gctgacggac agggaattta
     5881 ttgctgcggt gggtcgctca cttttattag caggtgtggt cttggtgacg attctgttgt
     5941 tgatgattcc caccattatt tggattcatt tgtatttccc tcgtttgaat cgttggctgg
     6001 aaaagctgct cctgctgccg tatgctttac caggagtgat tttggtaacg gctttgcttc
     6061 gaacgtatgc tgaaactggt atcccaatgt ttgtagtgtt ggtaggtgca ttgtttatta
     6121 cggcgttgcc gattgtctat cttagtctta acaaccagat gcgcttaatt aatctgaagg
     6181 aattagtgga tgcagcagaa accttgggcg ccccgatgtc tacgattatt atccaggtgc
     6241 tgtttccaaa catccgtatc ggtgtgaccc tagtttctct gatgattttt tcttcggttt
     6301 ttggggagta catgctgacg aacctgttga ttggcggtcg atttcagacg gtacgcatct
     6361 atatgctgcg gcggatgaac gaaaacggcc atttggctag cgctgtgatg gttttgtatt
     6421 tgctgtttat ggtgggaatt gctttaacta cctttatgct gacttatcga caaaagcaac
     6481 agctgtccct taagcagacg agggaagaga agcagtcgaa aaagaagccc ttaaaggagt
     6541 tgtgacggaa aatgttttta gaaatacatg atctcaatct gttttttgat gaaaaacaga
     6601 ttttagctat tgatgaaatt cagattgaaa agggtgagct ggttacttta ctaggtccca
     6661 gcgggtgcgg aaagtctacg ttgctgcgct gtatcacggg attggaacaa ccaaagagcg
     6721 gtgagattgt gctggacaat gagaatattg ccgataagcc tacgaaggat cgcaatatcg
     6781 gctttgtttt tcaacagtat gcattgtttc caactatgac tgtttttgaa aatgtagcct
     6841 ttggtttgaa ggtaaagaag ctgagtcctg aggtgattca ggaaaaggtt tttgagatgc
     6901 tatcattggt ggatatgacg gagcaggcag ataaaaatgt ccagtttctt tctggcggtc
     6961 aacgacaacg ggtggcactg gctcgctcat tggtcactga accgaaagtc ctgctgctgg
     7021 atgagccatt aagtgctttg gatgcccgaa ttagaaaaca gctgcagcgg gatttgcgcg
     7081 ctatccagca atcgctaggg atgacgatga tttttgtgac tcatgaccaa gaagaggcga
     7141 tgcgaatcag tgatcggatt tttgtgatgg aagctgggcg ggtggctcag gtttccacct
     7201 cgaaggaatt gtatcagaat cctcagagcc gatttgttgc ggaatttatc ggcaactata
     7261 atcgtttcga atggtatgag ctgaatcagt atacccacga gttttccgct aagggcaatc
     7321 gctgcattta ttatttaaga ccggagctga ttcaatttga accggttcct cagggggtta
     7381 agattccggt tctatggaag gaatcattta ttttaggcaa cattcaacgg tatgtttttc
     7441 aaacgctgga gggcaaagaa attttagtgg atcggttgaa cgatcgggaa gagaaaatta
     7501 gcgatgcctt atacattcag ctgaaagata ttctagcaat ccctgtggaa attgatactc
     7561 agcattttgc acaacaaagc tagtgtatag tcggcaaaca tttctggctc tttgtcaaat
     7621 aggactgata gagtgatata agaacatcta aggtagaatt gattttctgc tttagatgtt
     7681 tttttatgtt taataagtga tcagatgatt gattaagaaa attctggttc tcatcctaac
     7741 ccgtaagcta ttcgtttcaa tgctttgatg tgggtatgca gattttctat tttgccattt
     7801 gagtaaggca actctaaggc taaacgaatg ccggattcat agtttataaa cgcttttttt
     7861 atatgatgca gttctcttgg aaccgacttg ggcattgttt ttacaagctg aaagaagatg
     7921 tccgcttctt tgtcataaaa agcttgaaga atttcttgat agtaagcata agctattcgt
     7981 agttcatcag aagtagaaag tagacgttct acgacttcac tttcggttaa ataggcccaa
     8041 ccaaaattac gccattttgt ccatttatca taaacaagcg tgtcctgtcg tttgatcaga
     8101 agtcgagata aagccttcac ttgtcgatag tgtttggctt gtattggatc gtttcggtcg
     8161 aagcttttca tgactcatag tcaaatatcc ttaaaactgc gaatgaggtg cttgacgatg
     8221 tgaaagcgat ccacaacgag tttggcatga ggaaagcact cttttatcag caaagcataa
     8281 gaggcgttca tatctgttac gagtattttt acgtttaaac gtgcacgtcg ttcaaattgc
     8341 ataaaataag aaatgagatc tttcttctta cgtgaaggaa gaatatccag taatttccca
     8401 gtatcgccat ccatacaaga aaaagaatag ggaccgactt gatttttcaa tgaacgaaat
     8461 tcatctacca atgaacagcg aggcaaccaa tttattttca cgtttgtcat ggggagcaga
     8521 gaagcgagtg ttcgcgaaac ggttgaccaa gaaacgccgc aatgtttaga aatcgtcgtc
     8581 attgcttgtt tttcagtcaa ttcctccaat attttataat gtacttgttt ggcgataaaa
     8641 cagttttttt caacaatgta ggtattcgcc gtaaaatagg tgtgacagcc acgacagagg
     8701 taccgttgct ttgccaaagc aagataagtc cgttgattcg cacacttatt gagaaggacc
     8761 agcgattttt tctttccgtt cttaacgatc agcccatgtt gattcacctg tttacactga
     8821 ggacagggct gaatcgtttt aatgagcgtg gcagaaacaa cgatactgtt gattgaatgt
     8881 atttttttct tctcgacatg agtgatttga atgtttccat ctttgattcc tagtaatagt
     8941 ttgataagat gaagatcagg catacagaac gctcctttga ttgtgttgtg gtgattcaat
     9001 tctaacggag cttctgtatg ttttgttttt ttatgctcaa aaggactgat agaatgattt
     9061 actatcagtc ctagaaatta tagcgccaca tttctttcac ctttgacttt tcagacggca
     9121 gtttttatgc tgatactgta gagagagcag gacacagctt ttgcttgttt tgggtaaggg
     9181 ggaaagaaga tgaccaaagc acttattttt gattttgatg ggacgatgat tgacagtgaa
     9241 tggatgtttt acgagagtat tcatcactat ttactgaagc ctatttactg gagcagttcg
     9301 gctataaaat tccttttgca gactatcaac aaggtgttgg gacggcagca gaactgtcct
     9361 tttatgaagg gataagtcaa gcggttggga gaccagtgga tgctgaacat tttgagcagc
     9421 ggatcagcag cactggaaaa tcggagtagt ctcaattctg aactgcggga gctagactat
     9481 ttttttgaag cacatccgat gattaaggaa aaaattgatt cctttgtgac gatcgatgat
     9541 cttcaggtcg gcaaaccaga tccagaaggt tatttgttgt gcttgaaacg gctgagggtt
     9601 gaacaggctg ttgtagttga ggatagtcca gtcggtgccc aagcagcacc gcatatgaca
     9661 gttgatgtac aagcgttgga tgcagatttt ttcgccttta gcgggcataa aatgtgcggg
     9721 ccgacaggaa tcggtgtatt atatggcaaa cgtcaattgt tagaacaaat ggagccagtc
     9781 gaattcggcg gtgaaatgat agattttgtc aatctttacg atagttcatg gaaagagctg
     9841 ccatggaaat ttgaagctgg tactccgaac attgcaggag caatcgatta cttgaatgag
     9901 attggtatgg agaatattca tcgttatgag caagaactgg ttgcctacgt tttacctaaa
     9961 ttacaagcga tcggcggtat caccatttac ggaccgcaag atcctgaaca tcatactggc
    10021 gtggttgctt ttaatttgga tggcatccat ccccgtgatg tggcgactgc tctggatatg
    10081 gaaggtgtcg ctgtacgttc aggacaccat tgtgcacaac ggttcatcga cgatttctgt
    10141 ttgactaatt ggtcgtttaa acagtcgatg ataatgccgt ttgagcggct ccaatgtcgt
    10201 ggaatctttg agtaagagtt tccaatatcg cttgagccga cggtacttct tctgattttc
    10261 agattacgtg attgattttc gtacagttct atgagaatcg cctgcttcag ttcctttgtt
    10321 agttgatgat gttcatcaac taagtccgtt ttcgcagaaa aggtctgccc acaatcatag
    10381 catagaaaac gggtacgttt taattctaaa atagtcaact tttctttgac tttcaacatc
    10441 tgagtacgtg tcgtatagga accgttgcgg ataatacgaa tcgatccaca gtgttcacaa
    10501 tgggaaggtg agtcatccaa tctcccttta ataaactgcg cttgaacgcc tttatattct
    10561 ttttcaatca gccattcttc atcaaaaaat aaatgtttat ctgttaatcc gagtaatttt
    10621 ctagtatgat tatccataga gcgactcctt tggtagggtg atgttttggt cgactaaatt
    10681 ttaccaaaag tgagtctctt tttgagttga aacgaagaaa aagtgttagt agaaaatcgc
    10741 cttcgatttt ccaacaacac aaaatattat agaagccttt tttctaaagg atttcaaggt
    10801 gtctgtttgc atttattaaa aaaaagaaaa agtattggca a
//
LOCUS       DAJSKS010000092        10787 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJSKS010000092 DAJSKS010000000
VERSION     DAJSKS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.094"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            336..605
                     /gene="rpsN"
                     /locus_tag="ON396_001974"
     CDS             336..605
                     /gene="rpsN"
                     /locus_tag="ON396_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR3114047.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            620..799
                     /gene="rpmF"
                     /locus_tag="ON396_001975"
     CDS             620..799
                     /gene="rpmF"
                     /locus_tag="ON396_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR3114048.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            831..1208
                     /locus_tag="ON396_001976"
     CDS             831..1208
                     /locus_tag="ON396_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114049.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1229..1378
                     /gene="rpmG"
                     /locus_tag="ON396_001977"
     CDS             1229..1378
                     /gene="rpmG"
                     /locus_tag="ON396_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR3114050.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1402..2385
                     /locus_tag="ON396_001978"
     CDS             1402..2385
                     /locus_tag="ON396_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR3114051.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2415..2537
                     /locus_tag="ON396_001979"
     CDS             2415..2537
                     /locus_tag="ON396_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCR3114052.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2555..4078
                     /locus_tag="ON396_001980"
     CDS             2555..4078
                     /locus_tag="ON396_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCR3114053.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4102..4341
                     /locus_tag="ON396_001981"
     CDS             4102..4341
                     /locus_tag="ON396_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114054.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4534..4995)
                     /locus_tag="ON396_001982"
     CDS             complement(4534..4995)
                     /locus_tag="ON396_001982"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3114055.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5190..5408)
                     /locus_tag="ON396_001983"
     CDS             complement(5190..5408)
                     /locus_tag="ON396_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114056.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5526..7142
                     /locus_tag="ON396_001984"
     CDS             5526..7142
                     /locus_tag="ON396_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCR3114057.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7383..8723)
                     /locus_tag="ON396_001985"
                     /pseudo
     CDS             complement(7383..8723)
                     /locus_tag="ON396_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8999..9154)
                     /locus_tag="ON396_001986"
     CDS             complement(8999..9154)
                     /locus_tag="ON396_001986"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCR3114058.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9450..9782)
                     /locus_tag="ON396_001987"
     CDS             complement(9450..9782)
                     /locus_tag="ON396_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3114059.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10296..10486)
                     /locus_tag="ON396_001988"
                     /pseudo
     CDS             complement(10296..10486)
                     /locus_tag="ON396_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(10580..>10787)
                     /locus_tag="ON396_001989"
     CDS             complement(10580..>10787)
                     /locus_tag="ON396_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3114060.1"
                     /translation="VLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIGKV
                     LGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3586 a         1710 c         2093 g         3398 t
ORIGIN      
        1 ccagttaaac ataaaagatt gaataaaaag ttatagtttc tttcatattg ccgttttaag
       61 tagaggaaaa gctacttaaa acggtatttg tgtttgattg gactaataaa agtgaattct
      121 tatttttcag agcctaaact gcatattctt ttaaatgatt tgattatctc tatgtaccga
      181 taaattcgtt ttcaaatagt tatctattca tatgagtttt atttcatgtt atttattgtt
      241 gacaagagca actcttgttg gtaaagtatc agtgtcaaaa cgtaacgatt acgatttgag
      301 gtgtctaaat caaaataaaa ggagtataaa ggtatatggc aaaaaagtcg aaaattgcta
      361 aagcaaagaa acaaatggcg atgattgaaa agtatgcaga taaacgtcag gaattaaaag
      421 ccgcaggaga tcgcaccgct ctcgctaaat tgccaagaga ttcaaatccg aatcgtctga
      481 ggcttcgtga tcagacggat ggtcgtccaa gagggtatat gcgaaagttc ggtatgtccc
      541 gtattaagtt tagagaactt gcccatcaag ggttgattcc tggagtaaaa aaagcaagtt
      601 ggtaaaagga ggaaatatca tggcagtacc agcaagaaaa acatctaagg cgaagaaacg
      661 gttaagaaga actcatcaaa ctgtagtaaa accagaaatt tcatttgacg aagcgaatgg
      721 tgattataga cgtagccatc atgtatcttt aaaagggtac tataatggaa agaaagtaat
      781 taaaggagct tcaaaatagt ttaatatata acttttagga gggaatcaaa atggaaatta
      841 tttacccacc tttagttgaa gaaggattaa agtattatct tgaaacgacc cagcaatcgc
      901 tagataaatg cactttttat cgttcgatgg tagagagagg aattattact gaaacgggtt
      961 taccgaccca acaggcaatt gaaaatggat tagtcaaaga ttactatgag gaccaaggct
     1021 tatcatttga tgatttttta agaatctatc caatttttga agagtatgac gaggagttat
     1081 ttcaatgtat cgatggttat tgggagatac ctgttgatat gaaagaaaat ttagtttcac
     1141 aattggaatc tggcgagtta acttttgaag atgcacaaca aattcaagca tatcttgaag
     1201 atcgataagt attgaaggag gaagatttat gcgtcaaaat attattttgg aatgtgtcga
     1261 aactggagaa cgtctgtatc tcaccagcaa aaataaacgg aataaccctg aacggttaga
     1321 attaaaaaag tattcgccaa aattgcgcag acgggcaatt tttaaagaag tgaaatagct
     1381 aagtggtaaa aaggtgacaa aatgggcata ccagttacaa tcgtttctgg gtttttaggg
     1441 gctggaaaaa caacgctaat caatcaagcc ttgcaagtct caccctaccc cagggaagaa
     1501 attattataa ttgaaaatga gtttggtgaa gttgggattg atcacaaatt attagttcat
     1561 agcgaggaac gggtgttaca actaaataat ggatgtatgt gttgcagtct tcgcagtgat
     1621 ttattagcaa tattgatttc tttactggaa acaatagaag aacatcaaca accaatttca
     1681 caaatcatca ttgaaactac aggtatagca gatccccaac caatcattca aacactttta
     1741 acagcgcctc aactaagagg aagagtatat atcgatagtt tattgactgt tattgatagt
     1801 gacagttttg aacttattga aagagaacca caagccctta agcaacttgt catggcagat
     1861 cgcatttttg tgtctagaaa acaaaacttg aatttatctg cactaaaaaa attacagaga
     1921 gaaatcaaaa gtattaaccc tttatccgat attcgaatgt tcacacatag tgaaacgatt
     1981 caaaaaaagg atttttataa tttgaaaaaa tttaatcatc ccttagtttt tgaaagtgat
     2041 tcatcagaaa atcactataa tggcaaatac tatcatcaac accatcaagt acatgagttt
     2101 aaatcaattc tcttaacttc agatcaagac ataaaaaaag attttttact ccaatggatt
     2161 gactggctga ttttaaccaa ccaagggagc ctgtatcgtt ttaaaggatt gattgcttta
     2221 caagagcagg attttatagt tgccatgcaa gaggtaaacg aacaggttaa ttttcaatac
     2281 accactcgac aaaaagacca aaccactatt attctaatcg gaaaaatgtt ggatgaagaa
     2341 aggatcaaac agagttttga taaattactg gcggaaagca aataatgata agtagtgatt
     2401 gggggtaatt tctaatggag aaaacggata tatctagtgc atatcgacga cttaaaagcc
     2461 cgaatattaa aactagaaaa agggcattaa aaattatcaa agaacataaa cgaaataaaa
     2521 tgaagaaatt agcataatta aggaaggaaa aagaatgaag aaaatcagtg taggacttat
     2581 tgggatagca gctctaggat tattgggagc ttgctccagc actaatgatg caaaagtatc
     2641 gaatgataaa gatggaaaat tagaaattgt aacaacattc tatccaatgt atgatttcac
     2701 caaaaacatc gtcggcgatg aagcgaacgt ggatttaatg gttccagcgg gatcagaacc
     2761 tcatgactat gagccctcag ctaaggatat ggcaaaagct catgatgctg atgtttttgt
     2821 atatcataac gaaaacatgg aatcatgggt accaaaagct aaagaaagtt ggaagaaagc
     2881 tgggcctaat gtagtagaag gaacaaaaga catgatttta ttgccaggta gtgaagaaga
     2941 agatcatgat cacggagaag aagatcacca tcatgaacta gatcctcata cgtgggtttc
     3001 acccaaaatg gcaattaagg aagtttcaaa tatcaaggat cagttagtta aattgtatcc
     3061 gaaaaaagca aaagtatttg agacaaatgc tgaaaagtat ttgactaaac tgaaacggtt
     3121 ggatgctgac tataccacta gtcttaaaga agcgaaacaa aaaagttttg tgacgcaaca
     3181 cgcagccttt ggatatttag ctttggatta tggattgaaa caagttccta ttgctggact
     3241 aagtcctgaa gaagagccat cttctggtcg attagctgaa ttaaaagagt atgttaaaaa
     3301 aaataagatc aattatattt actttgaaaa gaatgctaat gataaaattg caaaaacttt
     3361 ggccaacgaa gctgggataa aattggaagt actgaatcct cttgaaagtc ttacaaaaga
     3421 acaaatggat aatggtgaag attatgtttc agtaatggaa gacaatctaa aagcgttaga
     3481 aaaaacaact atggttgctg gtgaagaggt agttccagaa aaagaagcga aggatgaaaa
     3541 gacagttgct aatgggtact ttaaagatgc cgatgtgaag gatcgcgaat tgagtgatta
     3601 tacaggcgaa tggcaatctg tttatccgtt gttaaaagat ggaattttgg atgaagtttt
     3661 cgattacaaa gcaaaaatca ataaagacat gacagctgcc gagtacaaag attattatac
     3721 tactggctat aagacagata ttgatactat caacattaaa gacaacacca ttgattttgt
     3781 agtcaacggt gaacatcatc aatacacata caaatatgtt ggatacaaaa ttttgaatta
     3841 tgaaaaagga aaccgtggtg ttcgttttaa ttttgaaact gatgatgcag gtgctggtcg
     3901 ttttaaatat attcaattta gtgatcatgg aattgcacca agtaaggcag agcatttcca
     3961 tattttcttt ggtggagaaa gtcaagaaaa actctataat gaattgcata attggcctac
     4021 tttctatcca gcttcattgt ctaaacatga aattgctcaa gaaatgatgg ctcattaaaa
     4081 ttcaattttt ggaggaaata catggtaaga aaagtattat tgtctatgga ttccaagaat
     4141 attgatgctt atcggcgtta tcgatttgca aagcaaatga gattaaatgg gagtacatta
     4201 gctgatattg ctgcaacttt acatgttagt attgacacag ttcatagagt attaaaacgg
     4261 gatcctgatt gttgttttat caaggtcaat actggaagaa gtagacgttt agcaaaacgg
     4321 cataatagct tggccaatta attgataata ccagagtaga tcatttttcc cttatttgga
     4381 tttgatctgc tttttattat gtcttgaatt gtcaaatcaa gcgcaacact ttatcaaaga
     4441 agacttcata aggtgttctc caatctaaac actttctagg tctgttattc agtttttcaa
     4501 tcatctgatt tatcgttgag ttatctaccg tagctatctc tttcgatttt ggcaagtatt
     4561 ctcgaatgag tctattcgta ttttaatttg ttcattgttg ccaagggcat gaggatctgg
     4621 aaaataaaag ggcatattat tcaatgcttc tgtaactgcg aaatgattgg agaattcttt
     4681 tcctctatct ggagtgatcg ttttgaccgc ctcatttgga atactgctta aaagttcaat
     4741 cattttctcg ctcacataag cggctttctt tctggggacc ttctttcctg ctaaaaggaa
     4801 gcatgatttt ctgtctgtca aagtcactaa acaagtacca ccagttttgc cagctacaat
     4861 atcggctttc caatgaccaa aatcacttct atcatctgcg gattttggac ggccatggat
     4921 cgaatgagta aggcaaatct ttccacgaga ctcgacatga ttttttgtat gtcttgtttt
     4981 tcctcgatgg tgcaatgaac ggatcaggcc tttattgccg tgtgagaggg gttctgtatc
     5041 gaaaagatca ttgcaaattg cacgttagat cgtgataaaa ctaattgaaa agtccatatg
     5101 ttcatgcttc aaacgattcg agatttcctc tggcgaccac tgaaaatcga caaataaatg
     5161 acgaacaatt gaccaaactc acggatcatt tagaatgtga taacgtccgc atttttttct
     5221 tcgataagca taattttttt ccgctttgga aggtgaatag tttttttctt gtttcttctc
     5281 agttcatgta aaatagtaaa cgggctatgc tgaatgtcgt tgccaattat actaatagaa
     5341 aatccttctt cgtgaagcag aaagattctt tctcgttcga gtatagtaag atgacgatac
     5401 tggctcataa ttattttcct tctaggagtg ttctcgacaa acttatacta gttggatatc
     5461 gattatgact catttttatg tgttgcactt aaagtgtaaa ttcaaggtat agaaagaggt
     5521 tccctatgaa aaaaatacct attacaatct taactggcta tcttggttca ggaaaaacga
     5581 tacttctaaa tcatattctg tacggtgatc acggaaagga aattgctgtc attgtaaatg
     5641 acatagggga agtaaatgtt gatggggtat tagtcgacaa gaaaggtttt aaaagaactg
     5701 gagaaaaact tgtttcgatg ccgaatggat gcatttgttg tacattacgc gaagatttga
     5761 ttgttgaact agatcaatta gtgcaatttt ctgacttgaa tcaaattgtt attgaagctt
     5821 caggaatcag caaaccaatt ccaattattc aagcaattat attagctaaa aaccctttag
     5881 acattgattt gacccaacga ttagagattg gtgccattat tatagtagtg gatgtaaaac
     5941 gtttttggga taatttcgaa acgggtgaaa agattcagga aagaaaagct gaaggtaatg
     6001 tagttgaaga agaacaggat attcgtggct tgttaattga tcaattcgag tgttgtaaca
     6061 tattactttt aaataatagt gacttagtgt caaccaagac gatttacaaa attaacggtc
     6121 taattgctaa gttacagtcc aatgccaaaa taattaggac aattaaaggt gaagtagact
     6181 tatcgaaaat tctaaataca aatcttttta atatagatga gacttcctat tcagtgggct
     6241 ggttcaaaga gttagagctt ggctatcaaa atcatattcc tgaaacagaa gaatatggta
     6301 tctcaagttt tatctatcga aatcggttac tccttgacgc acaaaaattt gcccatctta
     6361 cggataaaaa ttttcctggc aatattacaa gagcaaaagg gtttctttgg tttgcccaac
     6421 atagtgattt ttcgacgatc tttgaaacat cgggtagcta taaaagtatt gaatcattaa
     6481 attattgggt agctagttta cctaaaattg agtgtcaaat gattatacaa aaaaatcccg
     6541 aattgaagga agcctggcat ccgttgtttg gtgaacgcat taataaacta gtttttattg
     6601 gtatgaagat ggatcaacaa tgtattaagg aacaacttga gggttgttta actgattctc
     6661 ttaatgcaga taatttccga aaaatttcac catttccatg gaaaaagtct aatatgttta
     6721 gccagatatc gttaatgaaa tttttaagaa aagctggtgt caagataggc gatgtcataa
     6781 ttattggcat tttgattatt acctctttta ttcccttatt tgtattctct atagaaaagg
     6841 agaatgaaac aactgaagat gctatcaaat atgccatcgt tcgaattgat ggcaaggaaa
     6901 tggatcgctt cgatttggat aagatcaatc ataaactcgt gaattatcat cctgcgaagg
     6961 gacaatataa tattgttgag attaaagatg gaagaattcg agttaaagaa gataatagtc
     7021 ctgatcaaat cgcggttaga actggatgga tctcaaaacc cggtcaaacg agtatttgtt
     7081 taccccataa gttagttatt agtattgaga aaaaagaaag cacagattac tacatttact
     7141 aatctggaaa tagatgagga ggtttttaat aagaaaaaga tattattatt aataatagca
     7201 ggggctttat taagtgtgtt aagcgcttgt ggacaagaaa aagaagcggt gacaacaggc
     7261 acaagtatct ttaacgggac ctgcaatggt ttagttaaca gtcggttaga ttacttattt
     7321 tggctcttcg tcaatgagtg ttggtgaaga aattcaatag aataatcgag atactcacac
     7381 ggctagacag ctatcttgtc tagccgtgtt gttgttctgg ctttagtcag ttgggtagta
     7441 ttcttagttt tcttaaaata ttttcctcga tataaaaaga ttcgttctcg gtagttttga
     7501 aagtttctat agccataggc aattcgtttt agtactttga tatggttatt caaacattct
     7561 atagggccgt tggaataggg aacgcgcaac gctcgtttga tttctgtttg gtatttcttg
     7621 aaggtcgtga acactggttg gtagtgctct ggcaactcct tgtagtcttc ttttaggagc
     7681 gcgacgaatt caggaacatc ttgtcttctg ataacggaaa gaaaatcctg gtagacttcg
     7741 tagcctcgct ttaaggaatc atcataagat aatagccgat cgactatctc tgcttctgtc
     7801 agatgatctc gaaatgacgg acgccagtag cgatgctgac ctttcaattc ccacgcattc
     7861 ttttgaagta gtttccagta tttctttaag tgcttcgacc gacgatcccc ttttttaaag
     7921 gtcttcatta cttgaattcg gtggttggta aaagcccgac caatgtgttg gctaatatga
     7981 aagcgatcgg cgacgattcg ggcattcgga aaacactctt tcaccaatgt gcgatagggt
     8041 gtatagaaat ctgaaaccac tagacgcact tgttcacgga cttttatagg gtagcgcaag
     8101 aaatagtcgc gtaaaggatt gagttgccga ttttccacaa tatcaatcag ttgatgggtc
     8161 ttgccgtcca tcatgacaaa gctcatggct cccgagacat ttttcactga tttgaattca
     8221 tcgaaacaca ccacttctgg taacagctgg ctcaaagaaa tggttttagg ccgaaggctg
     8281 cgaagtattc gaatgacagt ggtcggagaa atgtgcttca tacgtgcgat caaagacata
     8341 gaaagaggtt cgcttaatag ctcaagaatg gcttgtttga ctctgcgagc aatggagcag
     8401 cgacgatcac taactgaatc ttctgctaaa aaagtctttc cacagtgttt acatttgaag
     8461 cgctgtttgt taatccgcag aatggtcttg tagttactga catcattcaa taagatggat
     8521 acttttttcc agccctattt aatgatcgtt tgttcatttt gatagtggcg atgaaagcat
     8581 tcctccggtt gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc attgattcgc
     8641 tctttgcgaa aggcatcttc atgaaagata agatttaggt ctagaatatc tagtaactct
     8701 ttggtaaact ctgaatagga catcttgaaa cactcctttg aatggttttc gtcgacttta
     8761 ttctaaagga ttttaaggtg tctgtttgcg tttattagaa aaaagaaaaa gtattggcaa
     8821 cagaaggcgt caccaacact agatattata gatcccttat tttcttcgct tcaaattgtc
     8881 aaggagtcat cataaatttt gtatggattc ttttgtttgg gtagtagtct tctaaataat
     8941 cttggattaa caccttcatc tctattttac ttttatattt gttggagtcg attaattctc
     9001 atttgattga tcgatgaaac gcttcaatta ctgcattgtc aacaggagtc ccagtttggc
     9061 tcactgaatg tgtgatgtgg tggtcgttca acagctcttg ataaataaaa gaccgatgct
     9121 ggctacctct atcggaatga attaccgtag gcaattctcc ttaataaatt tgtaatggac
     9181 ggacgacaat ctcttttttc atgtgtgaat ctaccatata gtaacgtaga atacgagttg
     9241 ctaagtcaat ataggtggac aaatagaggc gaccgtcatt acatggaatg taagtaatat
     9301 ccatcaccca aacagcatca atttggttct gttcaaatta tcgattcaac atttttttac
     9361 catttctttg attgtttttt ctctaccatg agaagatttg cgtcgatgaa atcctcttgt
     9421 gcataaattc atttcacgca taagaacagc tacaacacgt ttattgattt cgattccttc
     9481 atcgtaaagt ttttgagtga tccctggaga tccgtatcga tctttgtgtt cgtagaacac
     9541 tcgtcttcct cttttagtcg tttattttcc tgttcaagaa tgtagaaatc tgctttatca
     9601 ggtaatcttt ttccttgtcc agcaaaagca tttttcccgt atcgacgata ttctgaaacc
     9661 catcgggaaa ttgttggacg agagatattg tattcttcag caattttctt ggtagttact
     9721 tttttctcaa ggatatcctg tgaaatcttt agtttaaatt tacgatcata tgtttttctc
     9781 aatagagttc attccttccg gtaacgtaac tgatagtcaa gaaaagtgta tcatagccca
     9841 acttagtatc agggtttgtc aactaaactg tggaagttga tagataagag tttttaacaa
     9901 ccaaaattct cttgactatt tttgaaatca gacaaatttt gattggacac atagttgaat
     9961 aaatctaccg tcgttacgga aggtaaatcg catgcttttc aattctagag gagaagtcct
    10021 cgttgaagta ccatagccca gttctggatt cgacctcctg cccacttcat acgtaaataa
    10081 agtaatttta ggagtgagac tgtgacaaaa gttattttta agctaaaaac gaattaaaaa
    10141 acgttgataa ttaaaatcat tcgttttaga ttctatgaat cattatagaa cgctgtaaaa
    10201 tcgatacttt ataattataa agaagatagt ttcaaattta ttgttcgaaa tttgaaacta
    10261 tctttttact tatgtcacag actcccttag ttgagttatt ctttaatttc gaatttcgag
    10321 atagctagat atattgcaag catacattct tgataacaat cttgatcata ttttccagtt
    10381 ctcaggattg cttatgaatc aaagagctaa atcttttgag aattgcctca acagcttctt
    10441 catttccagc ctttgctaaa atgaaatctt cttttaaaga attcataaat ctaccttctt
    10501 cccatggact tataatctat ttctttggga aggaggagaa cgagcaaaga agcatacaaa
    10561 attagttatt atgcagatat taaaccagca ttttttgaat ttttttgtaa agtcgatgtt
    10621 ttaaatttgt cgcaccctgt cttgtaatcc ccagaacctt tccaatttct ttatcagttt
    10681 tatcaaagat aaatttttcg ataagaaaaa tttgttcagg ttctttcaac aaaggaagga
    10741 tttttgctaa ttttatatct tcaacaagga gagtcacatt taaaaca
//
LOCUS       DAJSKS010000093        10737 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJSKS010000093 DAJSKS010000000
VERSION     DAJSKS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.095"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            81..1226
                     /gene="tgt"
                     /locus_tag="ON396_001990"
     CDS             81..1226
                     /gene="tgt"
                     /locus_tag="ON396_001990"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR3114061.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1323..1703
                     /gene="yajC"
                     /locus_tag="ON396_001991"
     CDS             1323..1703
                     /gene="yajC"
                     /locus_tag="ON396_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR3114062.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            2008..4605
                     /gene="adhE"
                     /locus_tag="ON396_001992"
                     /gene_synonym="adhC"
     CDS             2008..4605
                     /gene="adhE"
                     /locus_tag="ON396_001992"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR3114063.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            4834..5940
                     /locus_tag="ON396_001993"
     CDS             4834..5940
                     /locus_tag="ON396_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCR3114064.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            5957..6985
                     /locus_tag="ON396_001994"
     CDS             5957..6985
                     /locus_tag="ON396_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCR3114065.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            6991..7470
                     /locus_tag="ON396_001995"
     CDS             6991..7470
                     /locus_tag="ON396_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR3114066.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            7486..8781
                     /locus_tag="ON396_001996"
     CDS             7486..8781
                     /locus_tag="ON396_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCR3114067.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            8792..9616
                     /locus_tag="ON396_001997"
     CDS             8792..9616
                     /locus_tag="ON396_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR3114068.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            9781..10680
                     /locus_tag="ON396_001998"
     CDS             9781..10680
                     /locus_tag="ON396_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114069.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
BASE COUNT         3486 a         1854 c         2374 g         3023 t
ORIGIN      
        1 agggaacttg caaaaagcac ttgaaacgat tatgatggtg ggagttgtca agatatttta
       61 tatagaaagt agggaaatga atgacgcaac ctgccattcg ctatcgttta atcaagaaag
      121 aaaaacatac tggcgcgcgt ttaggggaat tgatcacgcc acacggaact ttccccacac
      181 caatgttcat gccagtagga acattagcta cagtaaaaac aatgtcccca gaagagttga
      241 aagaaatggg tgctggcgtt attttaagta atacatatca tttatggcta cgccctggtg
      301 aagacttagt agaagaagct ggcggactgc ataaatttat gaactgggat caaccgattt
      361 taacagattc aggcggcttt caagtttttt cattaagtga tatgcggaat atcgaagaag
      421 aaggcgtgca tttcaaaaat cacttgaacg gttcgcgtat gtttttatca ccagaaaaag
      481 caatcaacat ccaaaataaa ttgggctctg atatcatgat gagttttgac gaatgtccac
      541 cattcgatga aagttacgac tacgtgaaaa aatcagtaga acgtacgtct cgatgggcag
      601 aacgaggatt gaaagctcat gctaatccag accgtcaagg attatttgga atcatccaag
      661 gagcagggtt tgaagatctt cgtcgtcaaa gcgcgaaaga tctagtcggg atggatttcc
      721 ctggttactc aatcggcggg ttatctgtcg gggaacctaa atcagaaatg aatcgtgtat
      781 tagaattcac tacaccgttg atccccgaaa acaaaccacg ttacttgatg ggtgtcggtg
      841 ctgctgattc attgatcgat ggtgttatcc gtggagtaga catgtttgac tgtgtcttgc
      901 ctactcgaat cgctcgaaat ggcacatgta tgacttctca aggccgtcta gtagtgaaaa
      961 atgcgaaata tgcccatgat ttccgtccaa ttgatgagaa atgtgattgt tatacatgta
     1021 aaaactatac acgtgcctat atccgccatt tgatcaaatg tgatgaaaca tttgggattc
     1081 gtttgacttc ttaccacaat ttatacttct tgttgaatct aatgaaacaa gtacgtcaag
     1141 caatcatgga tgataatttg cttgaattta gagaagcctt ttttgaagaa tacgggttca
     1201 ataaagaaaa cgcaaaaagc ttctaaagga aaatctttta aaaattaagt aaaaaactag
     1261 tgttatcagc atttttctgc taaagtgttt tttgtagata cagaattagt gaggtgaaaa
     1321 aaatgggaac attaccaatg cttgtgatgt ttatcgcgct attagcaatg tggtttttta
     1381 tgtcacgttc gcaaaagaaa caacagcaag aacgtcaaaa cttgttagat agcatgaaac
     1441 caggtgattc agtcgtaacg atcggcggat tacatggagt tgtttcagaa atcgacaatg
     1501 acaaacggac agttgtttta gactgcgaag ggatcttcct tgagtacgat cgtgcagcaa
     1561 tcaaaacggt caaaccaggg actgtcgtaa caaatgatgc aacgactact gttgaaacaa
     1621 cagaagaacc agctgagaca gttgaagtaa aaactgaaac aacagaaacg ctggaagaga
     1681 cagaagagaa ggaagaaaaa taagtatcaa agcttattta aagacgaggt agggcagaaa
     1741 gatgtcctac ctcatttttt attcaaaaaa gaaagagata agagcatggg atatattgct
     1801 caatattgaa taatcgggtg tgtcatataa ataataataa ataactttat atatttttct
     1861 atacaaacac ctgtaaataa agcaatttta tatttaaaaa taactgtgaa aaaaatcaca
     1921 aaaaagtgtt tacatagaaa aataaatatt gtatgatata catgtgaatt atttaacaaa
     1981 taaccatgac ccattaggag gataatgatg gctaaagtaa tagaaaaaga aaaaacgaag
     2041 acaattgacg tacaagcaat gattgatgat ttggcagaaa aagcaaatgc agcattgaaa
     2101 gaaatggaaa gctttgatca agaaaaagta gaccatatcg tgcatgaaat ggcaatggca
     2161 gctttggatc aacatatgcc attagcgaag atggctgtag aggaaacagg ccgcggtgtt
     2221 tacgaagata aagcaatcaa aaatatgtat gcgtccgaat atatttggca taatatcaaa
     2281 catgataaaa cagttggtgt gatcaatgaa gatgtccaaa aagggttgat tgaaattgct
     2341 gaaccagtcg gtgtggtttg cggagtaact ccaacaacga atccgacttc cacaacgatt
     2401 ttcaaatcaa tgatcgcgtt gaaaacaaga aatccaatcg tttttgcttt ccatccaagt
     2461 gcccaaaaat cttctgcaga agcagcacga gtggtacgtg atgctgctat cgctgccgga
     2521 gctcctgaaa attgtatcca atggatcgaa catccttcga tcgaagcgac ttctatgcta
     2581 atgaaccatc caggtatcgc gatcgtctta gcgacaggtg gagctggaat ggtcaagtct
     2641 gcttactcta caggaaaacc agctttaggt gtaggtccag gtaacgtacc agcttatatt
     2701 gaaaaaacag caaaaattaa acgtgcagtg aacgatttga tcgtttcgaa aacatttgat
     2761 aatggaatga tttgtgcttc tgaacaagct gtgatcgtgg ataaagaaat ctatgcagca
     2821 gtaaaagcag aattccaagc acaccaagtt tatatcgtaa aaccagatga attgcaaaaa
     2881 ttagaagatg cagtgatgaa cgaaggaaaa tatgcggtca atccatcaat cgttggacat
     2941 tctgcgatgg aaatcgcaaa attggcagga atcagtgtac caaaaggaac gaaaatgctg
     3001 attgctgaat tggaaggcgt aggcccagat tatccgctat caagagaaaa actttctcca
     3061 gttcttgcca tgatcaaagc taacaacacg gaccatgcgt ttgatttgtg tgaaggtatg
     3121 ttgaatcttg gaggattagg acatacagca gttatccatt cagaaaacga agagttgcat
     3181 gtcaaattcg gtcttcgaat gaaagcttgt cgtattttag tgaatacacc atctgcagaa
     3241 ggcggcatcg gtgatatcta taacgaaatg attccgtcat tgactttggg atgcggttct
     3301 tatggtaaga actctgtttc tcgtaacgtc tctgctgtaa acttaatcaa tgtcaaaact
     3361 gtagcgaaac ggagaaataa catgcaatgg ttcaaattac cgccaaaaat cttctttgaa
     3421 aagaactctc tattgtactt agagaaaatg gaaaacgttg aacgtgtcat gatcgtttgt
     3481 gaccctggta tggtccaatt cggttactgc gatacagttc gtgaagtatt ggcacgacgt
     3541 aaaaatgatg tgaagatcga agtcttctca gatgttgaac cgaacccatc gacaaatacc
     3601 gtatacgcag gaacgaaact gatggcagac ttcaagccag atacagtaat cgctcttgga
     3661 ggcggttctg caatggacgc agctaaggga atgtggatgt tctatgaaca cccagacaca
     3721 tctttctttg gtgcaaaaca aaaattcttg gatatccgta aacggacata taagatcgac
     3781 aaaccagaaa aaacacaatt cgtatgtatc ccgacaacat cagggactgg ttctgaagta
     3841 acgccatttg cggtcatcac tgacagtgaa acacatgtga aatatccgtt agcagattat
     3901 gcattgacgc cagatgtcgc aatcgtcgat cctcaattcg tcatgtcagt tccagcatct
     3961 gtcacagcag ataccgggat ggatgtgtta acacatgcaa tcgaatccta tgtttcagtc
     4021 atggcttcag attatacacg tggattgagc ctacaagcaa tcaagttagt ctttgactat
     4081 ttagaaaaat cagtaaaaac acctgatatg gaatcacgtg aaaaaatgca caacgcttct
     4141 acaatggctg gtatggcgtt tgctaacgca ttcctaggta tttgtcactc agtcgcacat
     4201 aaaatcggcg gggaatacgg gatccctcat ggacggacaa atgcgattct tttaccgcat
     4261 attatccgct ataatgcaaa agatccacaa aaacatgcga tgttccctaa atatgactat
     4321 ttccgtgcag atacagatta cgcagatatc gcaaaattct taggtctgaa aggcgaaaca
     4381 acagaggaat tagtcgaagc tttagcaacg gctgtttatg aattaggaaa atctgtcggc
     4441 atcaacatga gtctaaaagc tcaaggagtc acacaagaaa cactagatac aaccgtagac
     4501 cgtatggctg aattagctta cgaagaccaa tgtacgacag ctaatccgaa agaaccattg
     4561 atctctgaat tgaaacagat catcattgat gcttacaacg gataagcaaa agaaagcgta
     4621 gaaaaaagat aagaaaatag atgaaaaaga acttggaaaa cgttgtttct ccaagttctt
     4681 tttttaattt ccaagtaact gaaagttact tttgttattt aacatttagg tcaattttag
     4741 acagtttttg ttcaaaaagc gatatttata gtggacggta ttactgctat attaaggata
     4801 taaaatgaaa gcgtttaaaa aggtggcgta aagatgatta ttaaagaaga gatttttgta
     4861 cctaaagaat tagtgaaaga aaaaattgac ctgctcgttg aaaacctgac aacgatcaaa
     4921 gacgataatg gtgaattttt gttggacttt gatggactga aagtagatga caaaagctgg
     4981 acagtgtgga attggccaca aggtgtcggt ctatatggta tttataaaaa ttaccagatg
     5041 acaaaaaatc ctcgcgctta tcaagtggtc aatgaatggt ttgaggatcg tatggaagaa
     5101 ggcgctccgc ccaaaaacgt caatacaatg gcaccgctat tgactatggc ttacttgtat
     5161 gaagacacga aagattcacg ctatttgcct tatttagaac aatgggcaga atgggtcatg
     5221 aatgacatgc ctcggacaaa tgaagacggg ttacagcatg ccacatatgg accggaaaat
     5281 aagaatcagt tgtgggacga tacattgatg atgacagtgc ttcctttggc aaaaatcggg
     5341 aaactgttga atcgcccaga atatttagaa gaagctcgtt accagtttat gatccatatc
     5401 aaatacttga tggataaacg aacaggattg tggtatcacg gctggacgtt tgaaggaaat
     5461 cataactatg ccgaagcatt ttgggctcgt ggaaactgct ggttgacgat tgctattcca
     5521 gagatcattg aaatcttaga attatcgaaa gaagatgcat tgcgcaaatt attgatcgaa
     5581 acacttgaag cacaagtgcg tgcattaaaa acttatcagt ctgaaagcgg tttatggcat
     5641 acgttgttgg atgatccttc ttcttacgta gaatcttctg caactgcagg atttgcttac
     5701 ggtattttga aagcggtcca taaacgttat ttaccacaag aatataaaga agtcgcttat
     5761 cgtgctatcc aaggattgct tgaacaaatc gatgaaaaag gagaagttca aaacgtttca
     5821 atcgggacag gaatggggga cagtttagat ttttatcgga atatcggaat cacagcaatg
     5881 ccttatggac agtcgttaac ggtgctttgt ttgacagaat tgctcgtctc ttattgttaa
     5941 aagaaaggaa acccatatga aaaaaacatc actgctgttt gcagcaagtt cgcttagcct
     6001 aggtctgcta ggtctaagcg gctgcggaaa taattctagt gtggcaactg tcgcagatgc
     6061 agatgtaaat gagatagtta ccttgcgctt tgcttatgca agcaacagcc agccagtaat
     6121 tgatgcaatg aatgaatttg ggcgtttagt aaaagaaaaa acagatggtt ccgtacagat
     6181 ccagtatttc ccagatggac aactgggtgg agaacgtgaa ctgatcgaat tgacacagtc
     6241 aggagcagta gatttcacaa aagtaagtgc ctcagcgttg gaaagtttct ctaaagatta
     6301 ttcgattttt tcagttcctt atctattcaa tgatgaagaa catttcttta aggtgatgga
     6361 taatgaagaa ataatggagc caatctacca atcgaccaaa aatttaggat ttaccggatt
     6421 gacctattat gattctggtc agcgcagctt ttatatggta gacggtccga tccatacgcc
     6481 ggaagatttg gagggaaaga agatccgtgt gatgcaaagc gaaaccgcta tcaaaatggt
     6541 agagctttta ggcggttcgc ccgtacctat gggaagtgat gaagtgtaca cgtctcttca
     6601 atccaatttg atcaacggat cagaaaacaa tgaatttgtc ttgtatacag ctggacatgg
     6661 tggtgtggct aaatactatt cctatgatga gcatacacgt gtgccagata tcatcatcat
     6721 gaatgatgcc atcaaagaac agctaactga tgagcaggaa aaagccattg aagaagcagc
     6781 gaaagaatca acggtttttg aaattgaagc ttttgcaaaa gcaattgaag aagaaaaagc
     6841 tttagcacaa gaagaacatg gagtccaatt caatgatgta gataatacac cgtttcgaga
     6901 ggcagttgcc ccacttcatg atatattcaa aaacgatcct acctacaaag acctttatca
     6961 aaagatccaa gagcaaggag cgtagctgtg atgaaaatga ttttaaacaa ggtattagag
     7021 atcctgggag ctgttttgct tgttgggatg gtcgctgttg ttttatggca agtattttcc
     7081 agaacggttt taggaaatcc aaatacggtc acggaagagc ttgttcgatt cggtcttgtc
     7141 tggtttgcca tgttgtcttc cgcgtatgtt gtgggacaaa aagcacattt agccgtaacg
     7201 gttttaagtg aaaaattaac tggcaaaaaa agtaaaattt tagaagttgt tgttcaaagt
     7261 ttatttctgg tatttgctgc gatgatcatg gtgtatggcg gatggaatgc tgtcacattg
     7321 acaatggggc agatctcgcc ttctttaagt attcctatgg gatatgttta tctgtcagta
     7381 cctgtatcgg gggtactcat cattatctat agcctgatca atctaatcga tacattaagc
     7441 aacaagccag caattcaaga aaaaatataa aaatggagga ataaaatggc aatagaagcc
     7501 ggattagtgt tattttttgt atttgttgtg ttgcttgtat tagggatgcc gattgctata
     7561 agtattgcat tctcatcgat gatgacttta ttgttagtta taccgtttga tgtttctgct
     7621 tttagttcag ctcaaaaaat ggtgggaagt atcaatagtt tttcgctgat cgctattccg
     7681 tttttcgttc tctcgggcat tatcatgaat aatggaggga tagctaagaa actagtggat
     7741 tttgcgatgt tgttcgtcgg gcgcgtgccg ggggctttgg ctcatacgaa tgtgttaggg
     7801 aatgctttat ttggttccat ctctagctcg gccattgccg cttctaccgc gattggcgga
     7861 gtgttgatcc ctcagcaagt ggaagaagga tatgaccgga agtttgcgac agcggtcaat
     7921 atcgcgtcag ctccgacagg tatggtcatt cctccaagta ctgcttttat catgtattca
     7981 cttgttgctg gtggtgcttc gatctctgct ttgtttatgg gaggttatct cgtaggcgct
     8041 ttgtgggcgc taggaatcat gctagtagca ttcatcgttg ctaaaaagaa taaataccct
     8101 gttgtcgaaa aaggacagat ggctcatgct ggaaaaataa taaaagaggc gattcctagc
     8161 cttctattga ttgtcatcat tatcggtggt atcttgacgg ggatatttac agcagtagaa
     8221 gcttcagcga tagctgtcat ttactccctg ttgattgcta tgttttacta taaaacggta
     8281 aaactaaaag atatacctaa tatgttgttt caatcagtag caatgtcagg gacgatcatg
     8341 ttcttattgg ctacttcttc catgatgtct ttcgccatgg cgtttaccgg tattcctcag
     8401 gcaattagtt cgttgattat gggcgtaacg gataataaat ttattattct gttattagtc
     8461 aatcttgtgt tattactggt gggtatgttt atggatgtgg cgcctgctat cttgattttt
     8521 acaccaatat ttttaccaat cgtcacaagt gtaggggttg atcctgtaca ttacgggttg
     8581 ttttcaatca tgaatctgtg tgtaggctcc atcacgccgc cagtaggtac aggactatat
     8641 gtcggtgcaa gtgtaggagg cgtaaaagca gaggcaatgc tgaaaccgct tcttcctttt
     8701 tatggggtta tcttagtcgt cttgtttttg attacttact tcccgcaaat cgtcatgtgg
     8761 ctacccaata tgctgagtta ggagaatagt tatgttgaac attacaatta gaggacatga
     8821 tctttccgaa gtccaaacga tagaagatct tgcagaaaaa acaaaagaac aagggattca
     8881 tacactgcag ctcgccttag gtctttcgtt tcctgaaaca ccttctggcg caaatgagat
     8941 caattctggg atgggaaatt atgtcaaacg agttttagaa aagcaagaag tgaccgtagg
     9001 aattttaagt tgctatatca atatgattca tccagattta acgatacgac aagagttgct
     9061 gacaaagttc gagcagtatc ttcgctatgc ttcttccttt ggtgcgtcga tggttgcttc
     9121 ggaaactgga tgtgttttgc cggaaatcca atatacggaa gaaaatttca cagatgaagc
     9181 atttgctgaa gctgtgtccg ttatccgtcg cttggtaaaa gctggagaaa aataccaaat
     9241 gatggtgggg atagaaccag gtttgaatca tccggtgtat tctttagcaa gggtagagca
     9301 gctgatccaa gcagtagatt cggactatct aggaatcatt ttagatccga ccaatttgat
     9361 cacaagtagt gattatcaag aacaagtcca attggtagaa gaagcttttg aacgttttgg
     9421 tgaaaagata tgtgcggttc atttgaaaga ttttcgtgtc gaacaggaaa aaatcgtgcc
     9481 ggtaaatttg ggagacggtg tgatcgagta cacaaaaatc aaagaaatca tcatgaagaa
     9541 tcgtccgtac ctgtatgtgg ttcttgaaga aacaaaagat gatgggattc gttatggaag
     9601 aagtcttctt gaataatgta aagaagacgt tggttaacta atatttagaa tggagggggc
     9661 ggggctagaa gatgccacgt ctcttctttt ttataagagt atataaaagc agctcttttt
     9721 gattacaaaa aacgtggtat aataaaattg tgcataagtg aacaaaagga ggtgcggcac
     9781 atgctccaaa agaaaatcag cggtgaacaa gtcaatacga tggtttctga tcagtatgaa
     9841 atctatcatg tcaaagatgt cgtcagccaa aataaaacga tctatcatca tcatgatttc
     9901 tatgagatcc atgctacatt aaagggagaa gcgacttttt atttgaacgg acgacaattt
     9961 acgatcaaag caggaagtgt gctgctcatc cattccaatg atttgcatcg tatcgtccgc
    10021 caatcttcag atgtattcga gcgtgtctat atctttgtca ccccttcttt tctagaaagt
    10081 cgttccacgc catggtctaa tctatcggct tgttttcaac caatgggaga gaagaggagt
    10141 cgtgtattgc aaatagaaca ggaagtattg aagcaaaaat tatcatttat cgaccatcct
    10201 cctgataaaa aaatttacgg tgaagacata aaatatgaac aagctctggt agaatacttg
    10261 atttttctca atcaagttgt acaaaaagaa gagagtgcgc aagaacagaa tgctgtagtt
    10321 cctaatgagc gtgtggaaca aatgttacaa tatatctcaa aaaatttatc tcatccgctt
    10381 tctttagagg aaatggaaaa gcagtttttt gtcagcaaat accatatcac acgagaattt
    10441 aaaaagtaca ctggatttac gtttcatcag tatgtaatga agaaaaagct gatctatgcc
    10501 aagcacttgt tgcgagaata ccgcagctct agtgctgtat atagtaaaag tggtttttct
    10561 tcttatccgc attttctgaa agcattcaag agagagttcg gcataacgcc taaagaattt
    10621 ttgaaacgtg atacacagaa acagttggtt cattatgaac attttgaaga tgaacaatga
    10681 gaatgatcac taaaaaataa ccgcagtagc tgctgttgtt tacatagaag cagaaac
//
LOCUS       DAJSKS010000094        10566 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJSKS010000094 DAJSKS010000000
VERSION     DAJSKS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.096"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..387)
                     /locus_tag="ON396_001999"
     CDS             complement(<1..387)
                     /locus_tag="ON396_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114070.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGE"
     gene            555..872
                     /locus_tag="ON396_002000"
     CDS             555..872
                     /locus_tag="ON396_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114071.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            805..1284
                     /locus_tag="ON396_002001"
     CDS             805..1284
                     /locus_tag="ON396_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HCR3114072.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            1301..1477
                     /locus_tag="ON396_002002"
     CDS             1301..1477
                     /locus_tag="ON396_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114073.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            1734..2165
                     /locus_tag="ON396_002003"
     CDS             1734..2165
                     /locus_tag="ON396_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114074.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            2171..2488
                     /locus_tag="ON396_002004"
     CDS             2171..2488
                     /locus_tag="ON396_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HCR3114075.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            2607..3293
                     /locus_tag="ON396_002005"
     CDS             2607..3293
                     /locus_tag="ON396_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3114076.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            3268..4617
                     /locus_tag="ON396_002006"
     CDS             3268..4617
                     /locus_tag="ON396_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR3114077.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            4608..5081
                     /locus_tag="ON396_002007"
     CDS             4608..5081
                     /locus_tag="ON396_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3114078.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            5056..5583
                     /locus_tag="ON396_002008"
     CDS             5056..5583
                     /locus_tag="ON396_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HCR3114079.1"
                     /translation="MKIKRRMINMTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATK
                     SGAEFIDIDLIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSY
                     NGFDELLADFVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGP
                     ERSRATTVRNDNLPF"
     gene            5598..7910
                     /locus_tag="ON396_002009"
     CDS             5598..7910
                     /locus_tag="ON396_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR3114080.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            8157..8474
                     /locus_tag="ON396_002010"
     CDS             8157..8474
                     /locus_tag="ON396_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HCR3114081.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            8471..8632
                     /locus_tag="ON396_002011"
     CDS             8471..8632
                     /locus_tag="ON396_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR3114082.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            8635..8934
                     /locus_tag="ON396_002012"
     CDS             8635..8934
                     /locus_tag="ON396_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114083.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            8934..9248
                     /locus_tag="ON396_002013"
     CDS             8934..9248
                     /locus_tag="ON396_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HCR3114084.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            9241..9771
                     /locus_tag="ON396_002014"
     CDS             9241..9771
                     /locus_tag="ON396_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCR3114085.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            9768..10037
                     /locus_tag="ON396_002015"
     CDS             9768..10037
                     /locus_tag="ON396_002015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114086.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            9967..10170
                     /locus_tag="ON396_002016"
     CDS             9967..10170
                     /locus_tag="ON396_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HCR3114087.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            10167..10454
                     /locus_tag="ON396_002017"
     CDS             10167..10454
                     /locus_tag="ON396_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114088.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
BASE COUNT         3805 a         1727 c         2358 g         2676 t
ORIGIN      
        1 ttcaccaggt aatatatctt tcatatctaa aacttctgaa aaagcgactg agtagccagg
       61 agagattgct tgtgccaacg gtataggaga taaaaggcta cttttatgaa tgcttcccat
      121 atcaaaacca ttggcgtgat aatgtgaaca attaattttc ttaccgttgt aaagatacgt
      181 acattcgata attgagtttg gcatttttga tgggtttgca attacgccag taagaatcat
      241 tcgattactt ctatcatgct taactaaaaa ataggagagc gtaatttttc ttttatcttt
      301 aaataagtta tagagggata aaaaaaatcc tgaaatagaa atgattaaag taatagcgac
      361 ttggtaatcg ctcaaaattt ttatcaagta attcaccacc tttttactca aattatatca
      421 aaaaggagag aagaaataat gcaagaatta gtaattttga aaaataaaaa aagacacgaa
      481 cgccggcaag catgatcgtg tcagggaaat aaactatctt tctatatttt accacagaaa
      541 ggaatgaacc gtaaatgcta attgcaacgg atacactaga ccgcatcttt ttaaaagacg
      601 aatacaaact gcgcaatata gatgcgtcag gaattttagt atttgatctt tatgacaatg
      661 gaaaaattgg tatctatcaa gcaagtgata ttgaagaaac aaaccttgct ttcgaacaaa
      721 ttgatgattc tgtggaattg gatttagatg aggcaatcct agcttttgaa caaattgcaa
      781 aattattaaa ggaggcacaa aagaatggca actctttacc aactcagcga gtcatatatc
      841 aaagtcctgg aactggcaga acaattggat gaagaaattc ttcgcgatac tctcgattcg
      901 attaatgaag cgatcgagta taaggcagaa aacttagcaa aaatagttaa agaagtagaa
      961 gggaaagctg agttaatcga tagtgaaatt aaacgtctgc aggaacgtaa gacatcgctt
     1021 ttgaacaatg ctaagagtat caagcactat ttacaagagg aaatggaaaa gactggtaaa
     1081 acgaagatta aaggtgaatt attcaacatt ggaattcaaa ataatccagt atcggtcaat
     1141 gtaatcaatg agaacttaat tccaaaagga ttctttaccc ctgtgcctcc caaattggat
     1201 aaaaagcaat tgaaggagga actgaagcac ggagatattc ctggtgctga actcgtccaa
     1261 acaaaaggtt tgaggataag atgatgaagg aggagaaata atggaacacg aaaacgtaaa
     1321 agacgctttg aaagctgcta tcgaaatcgc ggaagctaaa ggaataaagg ttgacggcaa
     1381 accagcaact gtccatgaca ttcaaaattt gacgaaagaa catctttatt ttatcgccga
     1441 cttgcttggt ttgtcggaat tgtatttaga caaataaccc cacacggaag gagtggctag
     1501 ggtggttgta ttacaagtaa tagtctgcgt aacattcatt tggtcgtgca taccggtttg
     1561 tatatatatc atgaagaagc ttcgccctga gtcttacaaa ttatacgcaa tttacagcat
     1621 aacagtagct cttgcattta cattgtttgc aatttctagg cttccgtttt aatcgtggga
     1681 atgaattttt aatgaactaa attatctata aagcaaaaaa aggagaaaca acattgaaaa
     1741 acaaactagc aaaaacaaca gcaatcatcg gactagcact aggtagcggc gttatcggct
     1801 acgcagcaag caatgcgttt caagatttgg acacgatcaa agcaaatttc aacacagtcc
     1861 tacaatacgg tcaaacgaaa tcgcaacgtg tgtcagaact tgaatcacag ctatccaaca
     1921 acactcgcac acaggagcag ctgaaagccg aaattgagca aatcaaatcg gacaagcaga
     1981 aggaaattga agccaagcaa cgagaagtcg aacaaaagca acaggagatc gctacaaagc
     2041 aacaggaagc cgatagcttg cgccaacaac tatccacagt gcaaaacgac aaggagcagt
     2101 tagaacagcg cgtgagcgag ttacggcagt atacggatca aaaggtaggg gagttgggga
     2161 aatgaaagag ttgactattt atgacccaaa tatttcatgg gctaaacaca caattaaagt
     2221 ttcttttatg atttggggtt acaaaggata tgttacttac aaagtgggcg gtaacactaa
     2281 gggtttatca ttaatagcta ttgattcaga tgatctatat gatgctaatt tcgaggataa
     2341 cccagttaat ttccgtgacc ttgatgaaga ttggttctct atggaactta ctaacgataa
     2401 aggcgacagt acacttgtag aagatgaatt cgacagacta ggtgattata ttgtcggtgt
     2461 tgaaattatt gcgcacgaac cagagtagga gagtgaatac aaaatgatta aaagaatcac
     2521 tatatcagtt gaagaagata gtggatcaac aatgcagcat accgacaatc gaaaaacttt
     2581 tttaaccaat agaaaggaga attttgatgc agattaagaa ggcgacggat atcgaacaaa
     2641 ccaagggaac atatttgatc tacggtgctc caggaaaagg taaaacttca acggctaaat
     2701 attttccagg gaaaacactg atccttgata ttgatcggac ttcaaaagtc cttagaggtg
     2761 agaaaaatgt cgatatcgta tatatcgaca acgaagatac ctggaacgac tggggcaaca
     2821 cgttggctga tttaactacc aattatgtag gtgtttatga caacgtggtt gtcgataatg
     2881 tcagcgaatt ggaacggtgt attctgtcca gtttaggcgc agaaggaaaa aacaacggcg
     2941 ttccttcgca aggcgattat caatatatgc aatttagaat ggtgaacagc ctacgctata
     3001 tgaaaaattt agacagcaac cttatctgga ccgcatggga agaaatcgat ttgtggacgg
     3061 attcggatgg atcttcttac aacatcgctt tgccgcagat caatcgaaag attcgaaaca
     3121 atattttggg attatgtgac gttgttggtc gattgatggt aaaggaagac gatgaacgcg
     3181 gatttatttt acaagccaca gattcaacct atgccaaaaa tcaattggat aatagatctg
     3241 gctgtaagca aagtgagctg attttagatg gaattgtacg actaccagaa tgatttagtc
     3301 gatcgtgcaa gacaagccta tgttgatggt tacaaggcac catgtatcgt atcaccgtgc
     3361 ggttctggaa aatcagtcat ggttgcagag atcgctaaac gaaccacacg aaaaggcaac
     3421 agagtgttgt tcttggtcca tcgtcgtgaa ttgattgatc agatcaaaga tacgtttcaa
     3481 aagatgggcg tagataatca gcttgtaaat ttcggcatgg ttcaaacagt cgtacgacat
     3541 cttaaaacca tcaagaaacc acaactgatc atcactgacg aaaaccatca cgggttagca
     3601 gcgtcctaca gaaaaatcta tgagtattat tcggacgttc cgcgattagg ttttaccgcg
     3661 acacccatcc ggttaaacgg tagtgggttg ggggatgtga acgacttgct aattgaagga
     3721 gtatcggcta aatggttgat tgagaatcat agattagctc cttatgaata ttatgcacct
     3781 aaattgatcg atacagccga attaaagaaa gcttcgactg gagattttac aaagaaatca
     3841 atggataagg cagtgaagaa tactatttat ggcgacgtgt tgaaacatta taaaacgtta
     3901 gcagaaggtg aacaggcaat agcatactgt cacagtatcg atgcaagtaa acacactgct
     3961 gaaatattca acgatgctgg ttataaagca acacatatcg atgcaaaaac tccaaaagat
     4021 gaacgtgcag atattatcga agcgttcaga aatcatgaaa ttaaaatcct atgcaatgtt
     4081 gatttgatcg gagaagggtt tgatgttcct gattgttcga ctgtaattat gttgagacct
     4141 actcaatcgt tatcattgta cattcagcaa tcgatgaggg ggatgcgtta ccgaccagat
     4201 aaaacgtcaa tcatcattga ccatgtgggt aatgtgaacc gttttggatt gcctgatatg
     4261 gatcgtgaat ggagtttaga tacaaaaaag aaaacaaaaa ctgatagtga tttatctatc
     4321 gttcaatgcg cgttttgttt tggtgcttac gaacggccaa aaggcgataa tatttgtccg
     4381 tattgcggag agttgcagcc gattgaagaa cgaaagtcag agttagaaat cgatgaatcg
     4441 gctgaattaa tgaaagtcgg agaaacgaaa attactttga attttgagaa caataaatat
     4501 tacaacatga cggaagatga agcagaaagt atagaggact tatatgccat agcaagagct
     4561 aaaggattta aacctggttg ggcttatatg gctgctaaga ggaaggggtg gttgtaggaa
     4621 tgggcggtat ttttggtaaa aaagcagaag atgttcaagg ttcaataata aatggcgtaa
     4681 caataatagg tgatactgga aaaagagata gttcaggcaa tcaaattgta atcgctagaa
     4741 ataaaaataa cgaagttttt gaatcttctc tatctaatat aaaaagaggt gttgttaacg
     4801 ggacaggcga agagagtggc tacgttaatt taaaaaaagc taatgaaaaa taccatgttg
     4861 aaggaactta cgttaaggct ttgtctgtcg gtggaacaaa aggcgttaat tgggataaac
     4921 gaagaaataa atggaaagca cgattatatt tcaaaggaga atatgtctta caaaaggatt
     4981 tcaacaccaa agaagaagca atccaagcac gacttgcagc agaagaaaaa tatttcaaac
     5041 ctatcttaga aaaatatgaa aataaaaagg agaatgatta acatgacagg atttaactta
     5101 gacttttcaa aagcgcaaca agggaacgaa atcaaagacg gtacgtacga agtggtcgta
     5161 aacaaagctg tagaaaatgc aactaaatca ggcgcagaat ttatcgatat cgatttgatc
     5221 gttcgtaacg atgtggatca accgttccaa aataaacaca ttttcgcaaa aatctggaaa
     5281 gccaaagcta ccggcaaata taacgaaggt atgatcatgg caattgcaca agcgctacag
     5341 ttggaagacg gaaaatcata taacgggttt gatgaattgc tggcggactt tgttttgaaa
     5401 accgcctcag tacgcgtgaa aactgaagag tcaaatggat ataaaaacgt caatgtgaag
     5461 tcttgggata aaacgaacac acggggcgta atgaatcacc aattcaaaaa tggtgatgaa
     5521 ccatccttcg gaccggaacg ttcaagagcg acaacggtta gaaatgacaa tcttccattt
     5581 taattgaggt gtaatcaatg tatgaaaaaa ttccaaaaga gcttaaaaat ctcaaacaat
     5641 ggtgtgttta taagctagtt tgggatgaaa agcggcagaa gcacacgaag atcccttata
     5701 acgccaacaa cgggcataaa gccaaatcca atgacgagag tacctggtct gattttcaga
     5761 ccgctctcgc tgctattgat cactacggaa tgagtggact ggggttcttt ttcaaaccac
     5821 cttatttcgg cattgatatc gataatgctg aaggtgaagt tgagcgattt aaatcaggtg
     5881 atatagaaga aaatattatc tatgaattta tcgaaagcat gagatcctat gctgagtatt
     5941 ctcaatcagg tactggcatc cacattattg ctcgcggaga attgcccggc ggaagacgca
     6001 ggaaaggtga cgtagaaatg taccagaacg gccgattctt cgttatgact ggaaatgccg
     6061 catcaaaata tttagaaata actgagccga atccaaagga tatcaaacgc ctatatgatc
     6121 gctacgttgg agatgaaaaa atcatccaat tcaaagaaga aaatccgctg atgaataccg
     6181 ttgatttacc ggttgaagaa attatcaaac gtgctgaatc gtcttcccaa ggggcaagat
     6241 tcaaagtctt tatgaacggc ggttgggaag ctttttattc ctcgcaatcc gaagcggata
     6301 tggcgttcgc caacgacttg gctttttgga ccggtagaga ctttgaaaaa atggatgaga
     6361 ttttcagagc gtctgcaatg atccgtagca aatatgacca aaaaagggga tcggcaactt
     6421 acggagaatc tctactaaat aaggcaattg ctgatacaaa ttccgtctac aaccccaaac
     6481 ggaaatctga ttttaagatt tttatcaaag accaggaaca gccaaaagag gaaaaatact
     6541 attcgtacga cgacaccggc aacgcggatc ggtttactga tatctacgga acgttagtca
     6601 aatattcgta cattgataaa tcttggtatt actacgatgg gaaagtttgg ctacaggaca
     6661 ataccggcga agttcggaaa atgatcgata cgacagtaga catcatggga aaagaaccgt
     6721 taacgatccc tgaaggcgca gacgatgaaa ccaaagaagc tttgatgaaa gcgaaagaaa
     6781 aacacgtcaa gcgttctcgc agcaatgccg gaaaaaacgc tatgatggac gaattgaaac
     6841 atcgactatc cgtattgccg gaagagttcg acaaagataa aacgctgttc aacacacaaa
     6901 gcggctatct gtctttgcat gacggtcttc tacacgagca tgagatagac aaaatgttta
     6961 cacgtgtttc aaatgtcgag tacacagaat cggtcgattg tcctatgtgg gaagaattta
     7021 ttaatcagat ttttgacaat gatcaagaat taatcagata tatccagaaa tgtgtcggat
     7081 attccctgac cggatctacc agagagcaat gtatgttcat cctctacggt catgggtcaa
     7141 atggaaagtc ggtatttttg gaaattattt ctgaattgat gggaaactat gcgatgacta
     7201 tgcaggcaca aacgatcatg gtcaagcaaa gccaatcatc agcaaacagt gatattgcac
     7261 gattgaaagg tgcgcggttg gtcacttcgt cagaaccgaa tgaaggcgtc cgactggacg
     7321 aaggtctggt caaacaattg actggtggtg ataaagtcac ggctcggcac ctttatggaa
     7381 aagaattcga gttcgaacct gaattcaagt tgtggctggc aacgaaccac aagcccatca
     7441 tccgtgggac cgatgacggc atctggcgac ggttaaattt aattccattc actgtccaaa
     7501 tcccggatca caaaaaggat aaaaatctga aatttaaatt gcagacggag ttgcaaggga
     7561 tccttaaatg ggcaatcgat ggttgcctgt tgtggcagag agagggcttg gagaaaccaa
     7621 agtcggttgt tgcagcgagt caggattatc gaaacgaaat ggatcaaata ggtacattta
     7681 tcgaaacgtg ttgcgaaaca ggtccaggac taaaaatatc aggcggagaa ttatacaaag
     7741 tttatcgtga atgggcatca gataatggcg agcatacttt cactaacacg aagtttggca
     7801 gagagatttc aaagaaatat agcaaagaaa aagctggtgg atttatggtt tataaaggaa
     7861 tcacattaaa accaagaaaa tacgataacg ttagagaact ttttaaataa atctggaggg
     7921 ttctggaggg ttgagaacga aaccctccaa cccttagaaa cgttgatata tcagtattta
     7981 tattatattt ttctttcttt ctggagggtt ttaaaaaaat aataaagtat taaagtaaaa
     8041 aataaaaagt aaattatata aaaaagaata ggtttttggg tctgaccctc caaactctcc
     8101 accaaaatct tcctaaatgc tttgatacca acgattaaac agtctggagg gttacattga
     8161 aatcagaaca tcaaatacaa gacgagatca gaattgcctt atcaaaacat ggttgcacga
     8221 tttttcgatc caatgcggga caagtgacaa cgatcgaagg gcggcaattt tacgggatgc
     8281 caaaagggtt cccagatcta tgtggacatc gtaattctga tgggaaagca atttatatag
     8341 aagtaaaaaa tgaacgcggt aagttgcgtg aagatcaaaa aaggtttgct gaatttttaa
     8401 acggtcaacc tgtactatac ggtgttgcac gatcagtgga agaagcattg aaaattgtgg
     8461 aggaatcatc atgacaacag aagaagtgat tcaaatgcgt attcgaagcc ttcagcgtga
     8521 gattgacgat ctggaacgga caaaggcagt gatggtcaat gaaacggcga ggaaggcaat
     8581 cgatttgcac atagagaatt taagaaggga aatccatcga ttggaggaat gagcgtggat
     8641 aagaaagcag caatgaaacg aatcatcgaa ctgacacatt ctgagaattg gcaagaagac
     8701 aaagaaatag ttgcagaagt ccaaaagctc ggtaaatcaa tgtggagtga aaaaaccaaa
     8761 cggagaacac cgagaaaaat tgcaatctgg catggtgacc gaattctagt aacaggtact
     8821 gctgaacagt tatctgaaat tactggatta agcaaaaaca ttatctggga tagagctaaa
     8881 cgcgagaacg ttgattcgaa aggtcgtcaa tttaaacatt gggagaagaa ataatggagg
     8941 aactaatcac aaaagtagag cagtgggcaa aagataaggg attggatcaa gctgattcca
     9001 gcaagcaaat gttaaaaacg atcgaagaga ttggggaagt tgccgcttct ctagctagaa
     9061 aagatgaata tggtttaaga gacggaattg gagacgtagt agtaaccttg attattttag
     9121 ctatgcaaaa tgatatggat ttgtacgagt gtctgaacca agcatacaac gaaatcaaag
     9181 gacgcacagg aaaaatggta gatggtgtat tcgtgaagtc gagtgatttg gaggacagcg
     9241 atgaataaaa aagtattaat tgataaacaa gcattgattg atgaattaat gaaaatacct
     9301 ggtgtgggat ctaatagtga cgctttagaa acgattaaac gtttcccatc gtatgaaccg
     9361 cagaagccag ttgtgcctaa gtttgtggcg gaactactcg actattatcg tcagtcaaca
     9421 gatgttgatt tattagcttt attgataact ttccatgatt ggtattatcg caaaactaaa
     9481 gacggtgagc atgaagaagc aattgattgg ctagttgatc atcctgaaat ttatatgcgt
     9541 gcatggctgt ttggctacga gctcgagaaa gagccgttgt attgcgtaat aatcaatggt
     9601 agatatcttg tcaaagtatt cagcaataca aacgttgtca tattagttcc agcagatgaa
     9661 ttttcacagt acgttactca aaaatttcaa ttaactgaaa aacaaatcaa agcaattgat
     9721 gaaagatact ggccgtttgc tgtgccagtg gatgaggtgg tagaaggatg agcgacccat
     9781 acattactaa attagaaaag ttgtccgaag attttgagaa gaataaaaat aaactaacta
     9841 aagataaact tatagaactg gttaggttgg ttatcagagg agctattaca agagaaaagg
     9901 cgcagcaaaa catagaaatt tttgctcgta gtgtccgaga tggattagag aagaaagtta
     9961 aacaaaatga acaacagaca ccgcagaata acaaaactaa gaaaacggga actgaatgta
    10021 ctaaagacaa agtttgaaaa agaatatgga atttcagcag aagaaacata taaagtggca
    10081 agtcagtgtg ttgctgatgc gagcggcgct attcgtaagt ttggaatttc gatattaagt
    10141 gatgatcgta aatgggagga aaaagaatga aactaaaaga cggattttac tccagcagtc
    10201 atggtatcgg cggtttaatg ctagatatgc cgacaaagaa ccctaaaaca cgtgagaaac
    10261 caaaattcaa agtcggtgac atggttcgct gtgaagcaga aggattcatc tatccatttt
    10321 gtggatatgt agaacatctc tataatcact cagcaatcat tcgtattgaa aacacgatgg
    10381 aatgtgacaa gtggttagcg aaaagcaaag agaatttagc agtagcgaga ttggtggata
    10441 tggaggttat ataaacagcg aaaaaatgta cttctaagag aaaaatttat tttttataaa
    10501 aataggaaag tatcattatg aaaaagacca cagagataaa taaacctacc acataaaaaa
    10561 tgaata
//
LOCUS       DAJSKS010000095        10504 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJSKS010000095 DAJSKS010000000
VERSION     DAJSKS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.097"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            21..188
                     /locus_tag="ON396_002018"
     CDS             21..188
                     /locus_tag="ON396_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR3114089.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(248..601)
                     /locus_tag="ON396_002019"
     CDS             complement(248..601)
                     /locus_tag="ON396_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114090.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            1105..1524
                     /locus_tag="ON396_002020"
     CDS             1105..1524
                     /locus_tag="ON396_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3114091.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            1550..1756
                     /locus_tag="ON396_002021"
     CDS             1550..1756
                     /locus_tag="ON396_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114092.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(1984..2157)
                     /locus_tag="ON396_002022"
     CDS             complement(1984..2157)
                     /locus_tag="ON396_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114093.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            2133..2798
                     /locus_tag="ON396_002023"
     CDS             2133..2798
                     /locus_tag="ON396_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114094.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(2880..4046)
                     /gene="dnaJ"
                     /locus_tag="ON396_002024"
     CDS             complement(2880..4046)
                     /gene="dnaJ"
                     /locus_tag="ON396_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR3114095.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(4197..6026)
                     /gene="dnaK"
                     /locus_tag="ON396_002025"
     CDS             complement(4197..6026)
                     /gene="dnaK"
                     /locus_tag="ON396_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR3114096.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(6077..6640)
                     /gene="grpE"
                     /locus_tag="ON396_002026"
     CDS             complement(6077..6640)
                     /gene="grpE"
                     /locus_tag="ON396_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR3114097.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(6734..7777)
                     /gene="hrcA"
                     /locus_tag="ON396_002027"
     CDS             complement(6734..7777)
                     /gene="hrcA"
                     /locus_tag="ON396_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HCR3114098.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(8011..9176)
                     /locus_tag="ON396_002028"
                     /pseudo
     CDS             complement(8011..9176)
                     /locus_tag="ON396_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
     gene            complement(9192..9419)
                     /locus_tag="ON396_002029"
     CDS             complement(9192..9419)
                     /locus_tag="ON396_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR3114099.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            9903..10493
                     /locus_tag="ON396_002030"
     CDS             9903..10493
                     /locus_tag="ON396_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HCR3114100.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
BASE COUNT         2999 a         2146 c         1839 g         3520 t
ORIGIN      
        1 ttataaacta caagtggata ttgtgtcctg tatgtggaaa taaaacacga ttaaagataa
       61 gggaagatac tgaattaaaa aaattccccc tctattgtcc gaaatgcaga caagaaaatt
      121 taattgaaat aaagcagttc aaagtaactg tgattacaga gccagacgca aagacgcaga
      181 gccgataaaa tgagattaat acaatctcat tttatcggct ctttccgtta tgtatggatt
      241 cttttaatta gtcttcgatg tttcttgctt cgttgatacc gctggctaaa gattccatta
      301 aggatagttc tttgtctgta aagctatcca tgtatttctc tatctgtaat cgtcgggtgc
      361 tttttaccaa gttattagca ggtaagaaaa attcatcaac ggaaacatga agtaacgata
      421 caaggtcata aagaacttgt atgctggggt gttgcccttt attttcaata ttagttaagt
      481 accgtgggtc aatttcaatc aatgctccca cttgttcacg agttaaacct cgtttcaatc
      541 gagcttcttt aatggctaaa ccaaaggctc taaaatcata tttatcttct tttttacgca
      601 tagtagacca cctctataca ttttattgtt cctactgaat taaaaacagg tatagaaaaa
      661 cgtgttatat ggtttatagg tttatattta ataaaaagca ctactaaacg ccaataaaaa
      721 aaaccgttat atggtagtgc tatttacgct gttaaaatat tgtatattac ttccaaatgg
      781 cggtttgttg gaggtcaacg tcgccatgaa gtacatcata tacaataaat ttccttacat
      841 tgggttcttg tcaaaaaaag tcgtctatct gcaatagata agtacgtcca ccaatgtggt
      901 tttataaatc atatagatag aataacagaa gcatgtaaac agagaaataa atctgtttat
      961 atgctttttt ggctattcag aactttttta caaagtttat ttatcagtaa tgcaacaaat
     1021 ccccctttca cattgggact aagagtgaaa ggagataaac gagcaaggct cacttccttt
     1081 cctagacaga aagggggtga gattatgagg acatcttctt tcgagcatat cgtcagatta
     1141 cagttcaatt ctttaatgct gattgtcatt aagaacaaag tgaaaagtcg aaacagacag
     1201 cttgcaagac attctaaacg tgaagtattg ttttgtgaaa tagccgaaac gaaacaagtt
     1261 gaacgtggaa cgattgatac ctgtttttat gattgtgtat catttaaagt cttgcacttt
     1321 acaatttatg tatctgatga aacacttggt acagttttac atagactatc agaaaaacag
     1381 cgtagtgcga ttttattacg ttattttcaa ggaatgaacg acagagaaat ttcagaactg
     1441 taccatgtat cacgttctgc tatttgcagt agaagaacta gaggactaag gaagttaaaa
     1501 atgcttttga gcgaaaggaa gtaaagatta tggctatccc tcttttgaat tgatttgcaa
     1561 agcgtcaagc ggagatgaaa tggcagttaa agagattttg aagttttatg acggttatat
     1621 ttctaaatta tgcttgcgtc ctttttacca ctctgaaagt ggtaaaatca ttatgcaggt
     1681 tgatgaagaa ctaaaaggag aaattcatac agacatgatg aaagcaatat tgaaatttga
     1741 aatcggagtg aagtaatcaa ctgtataaaa aaatcgggag atacacagtt tcgtgttatc
     1801 tcccttagta gttttacggt catatcaagg ctcattcaat cgtggtaaat tcgtggtaag
     1861 atgagtgttt ttctatactt caaaatgctt gaatgtatag tgtttatgcg gataattgaa
     1921 aattagatat aaaatttaat aaaaaccaaa aatgcacggt cgccatgaag ggaccgtgta
     1981 tttttatctt cttattttct cacctaatcg tatcagccgc atcgcttgtt ttcctgcaca
     2041 aacaagcaat cgctctacct gttccatcgc ataagaaagt accatcggtt cattgatgat
     2101 atcaagtaca tagtcgatac caccctcact taattgagct tgatttttct tctccatatt
     2161 atgtagttgt gggacaactt tctgacaatc agcaattgtg tcaagaacgg atcaatcctt
     2221 tattggagtt gctcgaaagg gtcttctctt attacggaca ggctttgctc actgtacata
     2281 gacaacaaat cattatccta gtcaatcgga tttccagagc ctcattgcta tcattgcttg
     2341 ataaaatcca gacaaagttt aataaaatgt atcagttaca gctaaacttt ggaattgggt
     2401 cgctttgtta tacagagcaa gagacaccgc aatctttcct tcatgccaaa caagtttgcg
     2461 aatggatagc tttccatcaa tctgtgaacg agatccgctt ttttgaagat ttagacctcg
     2521 ggatcgtttt accagcaatc ccgtcagatc aacgaacatt atatgtaaaa cgtattttga
     2581 aaagcctgac agaagaagaa gtacatttgt tcaagaagac tttggcatgc ttcagtaaaa
     2641 ataacggaag catcaaaaat tgctcagaag aattatttat tcataaaaac accttgcaat
     2701 atcgattgaa caagttccat tctctcactg gttatacccc tagaaactat gacgattatc
     2761 atattctaaa gctggctttt ttgttggtac aaacatgaca actacaaaaa aaccaggcga
     2821 acatatgatt gttcgtctgg tttttaatta ttaatgactt agcgttttta ctagaaaaac
     2881 tacttccgct ttttcccgcc aaaagcatct ttaaacttat cgaagaaacc gtcttcttgc
     2941 tgttcttcaa ctttgatgcc gccagcttca gcgaatgcac gtaatgcttc tctttgttgt
     3001 tcatttagat ttttcggtgt gatcagtttt actttgacat gttggtcgcc tgttgcacca
     3061 ccacgtaact tcggagcgcc ttttccacgt aaacggaagt tcgtacctgt ctgtgttcca
     3121 gcagggatct tcaatttcac atctccatgg acagttggta cttggacttc atcccccaaa
     3181 gctgcttgta caaaacttaa aggcaattca tagtaaattt cagaaccttc acgatcaaag
     3241 atatcacttt cttctacacg gaagaccaca tacaaatcac caaatggtcc gccgttttct
     3301 ccagcttctc cttgaccagc taaacgcatt tgttgtcctt cttctacacc agctggtaca
     3361 ttcactttaa ctgaatgtgc tttcttctca tgtcctgagc catgacaggt tggacaaggt
     3421 tctttgattt ctttccctgt cccatgacaa acatcacaag tttgacggct catcactcgt
     3481 ccaagtggtg tttggcgttc tacattaatc gtacctgcac catgacattt atgacaagta
     3541 gttggttgtg tgcctggttt tgcaccgttc ccaccacaag tatgacagat ttcttcccga
     3601 ttatacttga tttctttttc tacaccaaaa atcgcttctt caaaagacaa gtcgaccgta
     3661 tattgcaaat cagcaccttg acgaggcgca tttggatcga ctgaccgacc gcctcctcca
     3721 aagaaagagt cgaagatatc ttcaaaacca ccgaagccgc caccagagaa gccgccgaat
     3781 ccaccaaatc cgccaccagc tccaccagca ccgtaatttg gatctgtgcc tgcatgcccg
     3841 tattggtcat atgcagcgcg tttttgcggg tcacttaaaa tctcatatgc ttctgatact
     3901 tctttgaatt tttcttccgc atcaggttct ttattgatgt caggatggta ttttttcgaa
     3961 agcttacgat aagctttctt tatttcatct tctgatgctc cttttgagag ccctaagact
     4021 tcataataat cacgttttgt tgccataggc tttcctccta ttggttctca tcattttcac
     4081 gtcgcttgtt atcatatcat attttgtttg aaacaaaaac cgatacttca aactattttt
     4141 caaaagaaaa agccaaagta gcaactttgg ccttctctcg aaattcagtg aactgattat
     4201 ttatcgtcgc catcgacttc ttcaaaatca gcatcaacga catcatctgc tccgccttga
     4261 gctgcttctg ggttttcttg agcttgttgt tgtgccgctt gttcgtatag tttaacagtc
     4321 aagttttgaa cgatttcgtt caatgcatcg cgtttagttt tcatttcttc gatgttgttt
     4381 gcttcaacag cggctttcaa ttcatcgcga gctgtttctg cttttttcac ttcttcttcg
     4441 tccactttgc cttctaattc tttcaatgtt ttgtccacag agaatagcaa tgcatctaca
     4501 tcattacgta ggtcaacttc ctctttacga gctttatctg cttctgcatt tgcttctgca
     4561 tcttttacca tacgttcgat ttcttcgtct gttagacctg aagaagattt gatcgtgatt
     4621 ttttgttctt tttgtgtacc tagatctttc gcagatacat ttacaatccc gtttttatca
     4681 atatcgaatg ttacttcgat ttgtggaata ccacgaggag ctgctggaat atctgtcaat
     4741 tggaatcttc ctagagtttt gttgtcagca gccattgggc gttcaccttg taatacatgg
     4801 atgtctactg ctggttgatt atctgccgca gttgagaata cttgtgattt acttgttgga
     4861 atcgttgtat tgcggtcaat caatttagtg aacactccac ccattgtttc gatacctaat
     4921 gacaatggtg taacgtcaag taaaacaaca tctttcacat caccagtgat cacgccacct
     4981 tggatagcag cacccatagc aactacttca tcagggttaa ctgatttgtt tggttctttt
     5041 cctgtttctt tacgtactgc ttctacaact gcagggatac gagtagatcc accgactaaa
     5101 ataacttcgt cgatttctga ttgtgataaa cctgcatctt ttagcgcttg acgtactggt
     5161 acttttgtac gttctactaa gtcagctgtt aattcatcaa attttgcacg tgtcaatgtc
     5221 atttccaaat gcaatggtcc agattctcct gctgtgataa atggcaagct gatttgtgtg
     5281 cttgatacgc cagataaatc ttttttcgct ttttcagcag cgtctttcaa gcgttgtaaa
     5341 gccattttgt cttgagacaa gtcaatgctg ttttcttttt tgaattctgc taccatgtag
     5401 tcgatgattt tgttatcaaa gtcgtcccca cctagatggt tgtcgccagc tgttgacaat
     5461 acatcaaaga caccgtctcc tagttcaagg atagatacgt caaaagtacc gccaccaagg
     5521 tcgaatacta agattttttc gtcgcgatct gttttatcta aaccataggc aagagcagct
     5581 gcagttggtt cattgacgat acgttctact tctagaccag cgatcttacc agcatctttt
     5641 gttgcttgac gttgtgcatc attaaagtaa gctggcacag tgataactgc tttttcgact
     5701 ttttcaccta aatagtcttc tgcgaatcct ttgatatatt gaaggatcat tgctgagatt
     5761 tcttgtggtg tatatgattt accttctaca tctactttgt aacctgcttc accgatatgg
     5821 cgtttgattg atgaaatcgt atttgggttt gtcacagctt gacgttttgc aacttcccct
     5881 acttggattt ctccattttt gaatgataca acagaaggtg ttgtacggtt tccttctggg
     5941 ttagcgatga tttttgcttc gtttccttcc aatactgcta ctgcagagtt agtcgtacct
     6001 aagtcaatac cgataatttt actcataatt aacgtctcct tctttaattg aaagtgtaag
     6061 ttttatatcc gcacaattat tgtgcaacaa tgaccatggt tggtcttaac acgcgatcat
     6121 gtaatttgta gccttcttgt agtacttcta caatcgtatc ggctggtgta tcttcagtcg
     6181 ctggaacagt ttgtactgct tgatgaagat taggatcaaa ggcttctcct ttagcaggga
     6241 ttttttcaat tccttcttct tccagtgcgt tacgcaaact ttccaagacc atttccacgc
     6301 cttttttcaa acctgcccct tgatcatctg acacttctgt ggctaatgca cgttccaggt
     6361 tgtcaattga tggaagtaat tttttggcta aatcttgtga acggtatttt tgtaattgct
     6421 cacgctcatt ttttcctcga tttgccatat ttgcgatttc agcacgagcc cgcaagtatt
     6481 tatcttccat ttcttctaac tcagctttta atttcgctgt ttcgaattct tccgcttcag
     6541 cttctgaaat acccgaatct tctgcagctt cgacatcgat ttccggttct ggctgagcat
     6601 ctgtcatttc tttatctagt tcttcttgat tttctttcac gatacagtct tcctttcatt
     6661 attcagtcag ctgtttgatt gctcttacta agaaagagag aacgatcagc tgcaaatagc
     6721 ttcgttattc acattacgag tttgacaagt caagcgaacg gtagtaatca gcaagtttag
     6781 ctgctagttc tcttcgataa acatcgacta gaccgaacat ccgagaataa ggcatactag
     6841 taggccctaa caaggctatt gtacctcttc catgaccatc tatctcgtaa cttgcttgaa
     6901 tcatactcat attctgcaat aaatcattgc ctaattcagc accaatcttg atatgaatat
     6961 tgctatccat cggagtgatc atttgtgtaa gtccgtctga gtctttcata aaagaaaaca
     7021 tcgatttgaa ctgatcaaga tcttgacggg gctcataatc caagagattc atttgtccac
     7081 tgacataaac cttatcttca aaaacatgac caagaacggt gtcaaataac tgagacatcc
     7141 cttctgtcgt ttggaaatat ttatgaagaa tcatcggaat ctccgtacga agtttatggt
     7201 acaccgtcat cagaggatct ccgactagac ggtcgtttac aatctgtacc attttttcta
     7261 gttcctgact tcccatatat tcgggaagag tgaacacttg gctttctaca ttccccatat
     7321 ctgttacgac gatagcaata atctgtcgat tattcaaggg tacgatacga aagccagtca
     7381 gtcggcggtc ttttatttca ggtcccaatg aaaaagtcgt atagcttgtc agctcagata
     7441 atatctcagc agattgctta ataatctcat tgatttcatg aaattctctg cctagagact
     7501 gacgaatcag cagcaaatca tcttctgcta cgcgagcagg ttttaacaaa tgatccacgt
     7561 aataacgata gcctaaagca gatggaatcc gcccagaaga agagtgagtc ttcaacagca
     7621 atccttcatc ttctaatgct ttcatatcat tgcgaatcgt tgctgagctg gccttgactc
     7681 cctcagacat caatgttttg gaaccaacgg gcacgccact actcgtatag tgttggatga
     7741 tcagacgtaa aatgtcgttt tgtcgctttg ttaacatctt cctcacctct cattagcact
     7801 catatattct aagtgctaat ccacttataa atatagcaag aagcccttct cctgtcaaga
     7861 gaaagactcc attttttagc actctaaaca caagagtgct aattctaaat aattgatgta
     7921 aatccagtta tatcaacgtt tcaatagtcc tttattgtat ttttgaactt tttttattta
     7981 aattcagata aaaaacaaat attctttaaa ctatttttca aacaaaaagc gctcaaacac
     8041 attgttcccc aaaaaggtcc ctcttggtgt caaataaaca cggtcttctt cattaacaag
     8101 taacccttct tgctctaatt cccttagcac atcaccgtac accgattcaa ttgttttttg
     8161 atagcgatca tagaaatgtt ttttagtaat ccctgttttt ttcttagtcc taagaacatt
     8221 tcttcttcta tttgattttt tcgtgtcaat ttttcttctt ccaataccgg gcgttcattt
     8281 gctcgtaatg ggcgtaaata gtgttggatt ggtcctttgt tgcggtaacg aatatctcca
     8341 agataaccgc tagcaccagc tccaaaccca aaatagtggt cattgttcca gtacatgagg
     8401 ttatgttgag attcatgtcc ggagagccca aagttactta tttcatattg gtgacgtcca
     8461 tgcttttcca ttgcttcaat cgtctcttca aacattctgg tttccgtttc ctgatcaggc
     8521 agttcgagtc ttccttgtcg tacccagttc atgaacattg ttttgttttc gaggatcaat
     8581 gagtacaaag aatagtgagg cagatccaaa gccaaagctt tttccaaagt atccagatat
     8641 ccttctagtg tttgtcccgg taatgcataa atcaaatcga tgcttacatt ggtaaaattt
     8701 tctgcttcta aaaattgcat agtttgatac acatcttctg cggtgtgttt acgtccaatt
     8761 tttttcaata agcgattatc aaatgtctgc actcccatgg ataaacgatt gacgccatag
     8821 tttttcatta cttgaagttt ttcacgggtc aaatcacctg gattcgcttc tacagtaaac
     8881 tcattaccat tttgaaaagg caggatctct ctagcacctg ataacagtcg atcaagttgt
     8941 ttagcagata aagaagttgg tgtcccgccc cctacgtaaa gtgtttctaa attcgctgtc
     9001 ggatgacttt ctagcattat tttcatttca ttcagcagca tttccacata ctcatctact
     9061 ggctgccctt ctaaaaaaac tttattaaaa tcacagtaat agcagatatg ctcgcaaaat
     9121 gggatatgaa tgtacgcaga aaccttgttt tctgcgggat ttattgtttc tatcattgaa
     9181 attctttctc cttacgtcga aaagtcgtgc ctgttttcga ccttcttaaa atcgcgttca
     9241 agcaccaact cattgaaata agccgcatat ttagaatcaa ggaaacctag ctctaaaaat
     9301 gcttgcttat tgtcatcgga gctacacggt gcttttacag cctctcgata aacggtaatc
     9361 gcaaagctga ccttcggcat taagtcaaaa tctgcaaaaa tatgaaaagc tattttcaca
     9421 gattcctcct taagtgctca gaccacaacg ctttgcttac aacctctcat atacgcatac
     9481 taaacgtatt tggcgaacaa atccactaaa aaatccaaga atcgacttct tggatgattt
     9541 tttctttcat taaactggtt acctagttat atatctctag ggttgttttc atgtctgagt
     9601 gcccgatatg atttattttt cgtttctata attacttttt gccttccagt ttcttttaat
     9661 attgtactca agcaagtggg aattggttct tttgtcatcc atgtatgcaa aatgttataa
     9721 tgctggtaaa caaaaatgat tacctgtcat ttttatttag ttcgtattta attaagttga
     9781 ctatctgtat actctgtcta aaacttaaga attgttgttt tcgctgtcgc tacccaattt
     9841 taatgaatgt ctgtaggtta aaactgttgt ccttgttata gatagtaaag gagagcttga
     9901 aaatgaaaac tgcgattacg tttctattag aggaatatgc cgattgggaa ggtgcttata
     9961 ttactagcac actaaaccaa aatgagaaat ggcaaaacaa aactgcttct atccaatcag
    10021 aggtcacttc cattggagga ttaaaaacaa aaatcgatta cttaatcgaa caaatacctg
    10081 aaaaagttga cctttttatt atgatcggtg gcaattcatg ggacataaaa aatcatcatt
    10141 tatatcaaat aatcgataaa ttgttaaaaa cagacgtacc agtagctgct atttgtgggg
    10201 cagttgatta tttagctcag agcggattat tgcaaggtta caaacacacc ggaaatgcgc
    10261 aatttccttg gttagaatcg gaaaattatc agaataaaga agattttata gaacaacaag
    10321 ctgtgaaaga tcgtaatttg attactgcga atggaacagc tcctatagaa ttcacagact
    10381 tgattcttca agcagtccat tttgaaacag aagaaataag agaaaaaacg attcgtctat
    10441 acaaacttgg attctatgat tactataaaa agtacggaga tcctttctcc taatcattct
    10501 cttc
//
LOCUS       DAJSKS010000096        10421 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJSKS010000096 DAJSKS010000000
VERSION     DAJSKS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.098"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            258..533
                     /locus_tag="ON396_002031"
     CDS             258..533
                     /locus_tag="ON396_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114101.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            546..2624
                     /locus_tag="ON396_002032"
     CDS             546..2624
                     /locus_tag="ON396_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCR3114102.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2784..4670
                     /locus_tag="ON396_002033"
     CDS             2784..4670
                     /locus_tag="ON396_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR3114103.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4682..5629
                     /locus_tag="ON396_002034"
     CDS             4682..5629
                     /locus_tag="ON396_002034"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR3114104.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5649..6176
                     /locus_tag="ON396_002035"
     CDS             5649..6176
                     /locus_tag="ON396_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3114105.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6239..6892)
                     /locus_tag="ON396_002036"
     CDS             complement(6239..6892)
                     /locus_tag="ON396_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR3114106.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7024..7866
                     /locus_tag="ON396_002037"
     CDS             7024..7866
                     /locus_tag="ON396_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR3114107.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8024..8920
                     /locus_tag="ON396_002038"
     CDS             8024..8920
                     /locus_tag="ON396_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR3114108.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8923..9636
                     /locus_tag="ON396_002039"
     CDS             8923..9636
                     /locus_tag="ON396_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HCR3114109.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9656..10174
                     /gene="msrA"
                     /locus_tag="ON396_002040"
     CDS             9656..10174
                     /gene="msrA"
                     /locus_tag="ON396_002040"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR3114110.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQDYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10171..10398
                     /locus_tag="ON396_002041"
     CDS             10171..10398
                     /locus_tag="ON396_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCR3114111.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
BASE COUNT         3615 a         1776 c         2156 g         2874 t
ORIGIN      
        1 atttttgtcg cagccccatt tttttgttgt gataaaagaa caagaaaggc aggaatggga
       61 aagtaaacat ccttttgtca gaaactttta tctttcttaa gttttacttg atatctgata
      121 ctatttggaa tagaatgagg cgttgaaaaa tttatggaaa tttctaacta gcaataaacg
      181 atgaaacaat gaaatatttt tcatccaaaa agaactttgc tataatgatg gaagtttgtg
      241 tataggagat ctggaaaatg agagagagtt ttgaacaaca aaaaaaactg ttgcatgatc
      301 gctatggcgc cctttcgatg gatgaccgcc gtcaaatact ttgtaagttg cgtaaaagaa
      361 atatattgat gtatcgtcag ttggaacgtc tgaaacatga tctgcttcgt ttggagtcaa
      421 aacgtgtaca gtgtgaatta gaggggaacc aaacacaggt tgaagtagtc gaaaccaaaa
      481 ttttaaagaa aaaagaacaa tttttaaaaa tgcttacaca aaataaaaaa taaaggggat
      541 ggacgatgga atttgctgag ttagttattg tatttgcagt aacgatcacc ttatcgaaca
      601 tagctagccg cattttgcca atgattccag ctccactgat ccaaattttc cttggagtgg
      661 tattaggtct gacagagtgg ggacaatcaa tcgattttga acctgaattg ttcttagtta
      721 tgattattgc accattgtta tttagagaag gagaaaaagc agatatttct tcgattctca
      781 aaaatttcgg gacaattttg ttcttggcat ttggaggagt cattttgacc cttgtcgggg
      841 taggtgcgac gttatcattc ctgttgccaa gtgtcccact tgctgcctgc tttgcttttg
      901 gagcagcgtt aggtccgaca gatgcggtag cagtcagttc tctttctgga cgagtgaata
      961 tccctaaaaa ggcgatgcat attttggaag gagaaggtct tttgaatgat gcatctggtg
     1021 ttactgcctt ccagtttgct cttggtgcgc tgatcaccgg atctttttca gcagtaaacg
     1081 cggggatgag tctagtagtt tctagtattg ggggagcttt gatcggcttt ttgcttgtct
     1141 ggttcaaaca aaaaataatt catttgatcg aaaaagcatc agcacaagat gtcacagggt
     1201 atctgctgat cgaacttttg cttccttttc tagcctatgt attggcagag gcatttgatg
     1261 tatctggaat cattgctgca gtggcagcag gtattctcca agcttctggt tttaggaaaa
     1321 tctctgtgtt tgatgcagaa ttgtcgagct tgtcccacag tacatggaca actattgctt
     1381 ttacactgaa tgcattagta tttatatttt taggaatcga attgacgcaa gtgttctcgc
     1441 cagtatgggg agacggttta tatccaaacg gtcttttatt agcgattatc gtattaatct
     1501 ctgtcatgct ttttgtgatc agattcattt ctatcagtct tttttatgta ttcaaagatg
     1561 gcagtaaaaa gttcaaaaaa cagttgaacg agatcttgat cttaacattt ggcggtgtaa
     1621 agggaacagt cagtttggct acgattttta ttttgccatt ttcaatcaat aatatgatgt
     1681 tttaccagcg gtctttgctt ttgtttctga cggcgggtgt catcttagtg actttggtca
     1741 tagggatcat tgtcttgcct atgctgacgg aaacagagga agcgaaaagc acggatttga
     1801 atgctttgat gattttagaa gaggtcgttg aagttttgcg aaaagaaata aaggaaattg
     1861 atcgtaatac aaaagaattt ttagccactg aagcagtgat agaaaattat caagaacgga
     1921 tacgtgatct gtacttggaa gatttaaccg atgatgaaaa gcaagaagta caagaaatac
     1981 aagcactgat tttgtcgatc gaacgggatg gattggatga aagttatcgt tcgggcaagc
     2041 tttcttctaa tggttatcgt ttttactccc gttttctttc tcgattcgaa cattctatca
     2101 caagccagat tttgtctttt atcggctttt ggttcattgt tgtccgtagg ttaatgcgaa
     2161 tcgttctcca tccgaaaatg ttttggcaaa gaagatatgc tgatcgtcag acatttatta
     2221 gtgatgaaga tatccaagaa atcagagaga catatcaaaa aaatactcag ttgattattg
     2281 aaagtttaga taacttgact gatatttatg atgaaacgat ggttcaattt tttatccagc
     2341 aacgcaaact tgaaggaata aaaatgaact cggggaattt gatttcttcg tggatgatcc
     2401 agcaagatag tctgtttacg aaaaaaatgc taagaggtta ttatcttgaa agaaaaatca
     2461 ttgatgaata cgaagttgct gaaaaaatca cgacgttttc tgcgaacagc tatcgcagga
     2521 atatcaattt gttagaatct tatacgatga acaaaccatc tgataacttt tctttccgat
     2581 ttgcgtttcg agcaaagtca aaaaaaacga gtccaataaa ataaaaaggt aaaaattttc
     2641 aagttttata aaaaacaaaa catatcagaa caggatcgaa tcggtcctgt tctttttttg
     2701 agaacacaaa aagaacaaat accaaaaaaa ttttcctgaa gttcactatt tttatgatac
     2761 aatatccaag ataaggaggg aacatgttga aagaattaaa agtaactgac ctcaaaaaaa
     2821 cttatggtga aaaagatttg tttgaccaga tttctttttt ggtccacgaa aaagaccgta
     2881 tcgggttgat tgggacgaac gggacgggta aaacaagtct cttaaatatt attgccggta
     2941 tcgatagcag cgacggtgat cggcaaacag tgttttatcc aactgattac cgtattggtt
     3001 atctttctca agcagctgaa ttttccgatg aattaactgt tctccaagca gtctttcaag
     3061 gaaacagccc cttgatccaa actgttcgag catatgagga agcattgatc gaactagggg
     3121 aaaacggaga agaccctgat gtgcaaaaac gttatgctaa agcagaagaa caaatgaata
     3181 aagaagatgc ttggactaca gataccaacg caaagattat tttgcaaaaa ttaggaatca
     3241 gtgaattaga taaaaaaatc agtactttat caggtggtca gaaaaaacga gttagtctgg
     3301 cgcaagtgtt gattgatgaa cccgatttac tattactaga cgaaccaaca aaccatttag
     3361 attatgaagc gatcgagtgg ttggaaaact atttgaaaca ataccgtggc gcactattga
     3421 tggttaccca cgatcgttat tttctagata gagtagcaaa ccgaatcttc gaactttcat
     3481 ttggaaaatt atacgagtat aaaggaaatt atgaagctta cgtcttagaa aaagcagaac
     3541 gtgatcgtgt agaagtagaa caagaagaaa agcgcaagcg gctttataaa caggaattag
     3601 cttggatgag ggctggcgta caggcacgtg gcaccaagca gcaagcacgg atcaaccgtt
     3661 tcgaagatct aaaggagaac ttgtaccaag taaaccaaga agatgattta gagctgaacc
     3721 tagcgacaca acgtttaggg aaaaaagtac tggaaatcaa agatggcagt taccgtataa
     3781 atgaccaaac actgctagaa catttagatt tattgattca atctcgggaa agactaggta
     3841 ttactggtaa aaatggtgcg ggaaaatcga cattgcttaa tattctagct ggcagaattc
     3901 ccctggatag cggtacaatg tctatcggtg aaacggttca tcttgcgtat tatacacaag
     3961 aaaatgaaga aatggcacca gataaacgga tgattgctta cttacaagaa gctgctgaag
     4021 aagcaaaaac agcagatggc agtcaaatcg gagtagcaga attacttgaa cgatttttat
     4081 ttccaagatt tatgcatggg acactgatag gcaaactttc aggcggagaa aaacgccggt
     4141 tatttttgtt gaaactgctg attcagcaac ctaatgtttt attattggat gaaccgacca
     4201 atgatttgga catagcaaca ttgacgattt tggaagatta ttttagaagt tttccaggtg
     4261 ctgtgatcac tgtttcccat gaccgttatt tcttggacaa ggtagcagat aagttattag
     4321 tattccaagg aaacggaaaa caagaacttt attacggaaa catgtccagt tatttactga
     4381 agcaaaaaga gacccagcag cctgctgaaa aagcgaaacc aaaaatacag agcaaggaac
     4441 ctgctgaaaa gaagaaatta tcttacatgg aacaaaaaga atgggaaacg attgaagatg
     4501 agattgcaga acttgaagaa aaaatctcat tgctccaaga agagatgaat catcaaggag
     4561 ataatttcac tcgattacaa gaattgcaaa acgacgtatc tgaaacagaa gcacaacttg
     4621 aagaaaaaat ggcacggtgg gaatacttga gtgaatgggt agaagattag gaggaaaaag
     4681 gatggaacaa gcgtatcttg acttaggaag aaaattgttg gaagaagggc atcgtaaaga
     4741 agacagaaca ggcacaggca cattaagctt attcggatac caaatgcggt ttgatctgca
     4801 aaaagggttt cctttgttaa caacgaaacg agtacctttt ggattgatca aaagcgaatt
     4861 gctttggttt ttaaaaggag atacgaatat ccgctatcta ttacaaaata aaaaccatat
     4921 ttgggatgaa tgggcatttg aacgctacat caaaagtcca gactataatg gaccagatat
     4981 gactgatttt ggccatagat gtttgacaga tcctgaattc aatgaagaat ataaaaagca
     5041 gagcaagctt ttttgcgaga agatcctaac cgatgattcc tttgctgaaa catacggcga
     5101 tcttggacac atctacggct atcaatggcg tcactgggaa acaaaagacg gtggctttat
     5161 cgatcaaatc aaacaagtga tcgaagcaat caaaaagact cctgattcta gaagattgat
     5221 cgtttctgca tggaatccgg aagatgttcc tagcatggct ttgcctcctt gtcatacgat
     5281 gtttcaattt tatgttcaag agggacggct aagctgccaa ttgtatcaac gcagtgggga
     5341 tgtgtttcta ggcgttccgt tcaacattgc aagttatgcg ttgctgactc acctaattgc
     5401 acatgaaaca ggtttagcgg ttggcgaatt tgttcacaca ttaggagatg ctcatcttta
     5461 tatcaatcat atcgaacaaa tgaaagaaca attgagccga gaaatacgcg catttccgac
     5521 tttagttttg aatcaggaaa agcagtcagt atttgatttt gagatggaag atatccaatt
     5581 agaaggatat gacccacatc cggcgatcaa agctcctatc gctgtttaga atcagaaagg
     5641 atgagaaaat gttagctgca atttgggcac aggatgaaaa cggattgatt ggaaaagaag
     5701 accgtcttcc ttggcgtttg ccaaatgatc tgaaattctt caaacaaatg acagaagcaa
     5761 atacactagt gatgggacga aaaacattcg aagggatggg tagccgtccg ttgccgaatc
     5821 gtcagacgat tgttttgacg agagattctt cctataaagc agaaggcgtg catgtcatgc
     5881 atagtgtgga agacgtgctt gctctggaaa aagaaacaga cggtatcttt ttcatcgctg
     5941 gagggtcagc tgtttatcaa gaatttctgc cattttgtac gatattatac cgtacagtca
     6001 tccatcatgt attcgatgga gatgcttatt ttccacctgt tgactggtct gattggtcac
     6061 tgatcaatac tagtcaagga gaaatggatg aaaaaaatcg ttatccgcat caatttgaaa
     6121 cgtatcagcg taatgagaat cctgtaaaaa gcgggacagg cgaattgacg atataaaaaa
     6181 gctatggata gactagctgt tatttagagc tgtcccatcc atagctttat tttttttatt
     6241 aagtaaagaa taaaacagag aagtacataa aacctgcacc taacataaca aacaaatgcc
     6301 aaacaacatg catatagcgg atagaattca agctataaaa gaatgcgcct atcgtgtaag
     6361 aaagcccgcc tgctaccatc aaaataacac cagtcattcc aagagagtga taaagaggaa
     6421 cagcagccac gacacagagc caccccataa aaatatagat gatcgtagat acttttgata
     6481 ctctctcccg tttatgcaac gtcaacgctt tatatataat gccaagaatc gcaaacaacc
     6541 agattaatac aaataaagtc caacctagcc atcctcggat actcagcaaa caaaaaggag
     6601 tataacttcc tgcaatcaaa agaaaaatcg aactatgatc gaatacttgg aacacctttt
     6661 ttgcttttgt aaaaatcaat gaatgaaaca aagtagacgt taaaaataaa agaatcatca
     6721 tcgatccata gatagcataa gagacaatat ggagggagga atgcaagtgg gcacctttta
     6781 ctaaaagaac cactagtccc gcgatactca atccagtccc aattccatgg gtgactgcat
     6841 taaacacttc atttaatatt tcatacgttc gattaaattt cgttttttcc atgaaaacgg
     6901 ctcctttact aggtgtaatt aatgaaaaat cattatttgt ctgttatact aaaaatagaa
     6961 attgtcttac atgtagttta acatgaataa ggcatcttgt taaagaaaga gagagagaaa
     7021 ataatgacaa atgtaaaaat cgtaacagat tcttcatgta cgatggaaaa aaatgtacgc
     7081 gatgctttag gcattcgtgt catgccgcta tccatcatga tcgacggaac agtatatccc
     7141 gatgatgacc aactagacgg cgaaagattt atggaaatga tggcgaaagc aaaaaatctg
     7201 cccaaaacaa gccaaccacc aattggagaa ttcatctcga tgtatgatga gctaggagaa
     7261 gacggcagtg aagtgatttc gatccatatg acaaaaggct tgagtggaac agtagaagca
     7321 gcaagacaag ccagtaatct aacaaaaacg aaagtaacgg ttatcgacag tgataccact
     7381 gaccaaggat tatcttttca ggtaattcga gcagctgaat tagcaaaaga aggtaaaaca
     7441 gtagagaaaa tccttgaaga agtcaaaaaa gtacaagaca atacaaaatt atacatcgga
     7501 atatccacac tagataactt ggtaaaaggt ggaagaatca gccgaacaac tggtttgctg
     7561 tctagtgttt tgaacatacg cgtggtgatg aatttcgccc attctgaatt aatccctgta
     7621 actaaggggc gaggaaagaa aacgttcgat aaatggttta ataatttgaa aaatgaactg
     7681 aaagaattgc caaatgtgcg acaaatcggt atttcacatg cggatgcaaa agaattggcg
     7741 caagaattca aagagggttt gcaagaatta ttcccggaca tgcatatccc tgtactgcat
     7801 acgaatccgg ttatcgctac gcatactggt aaagacgcgt ttgcaatcat gtattatgta
     7861 gattagtcga aaagaggata agccctcagc ctgaaaggct tgcagcttat tctctttttc
     7921 tttataaaat taggaaaata attgttttct tttttcataa agactacctt tttgataaaa
     7981 tttttgataa cattagaacc aatcgaatac aagaggtgaa aaaatgaaat taacgaaacg
     8041 acatttgatc ctcgttttgt tcccattatt aattgcgatc atcatctatg caggtttagc
     8101 tgcttctata ccgaaagctg gcccactaat gaaaccagta actactgtga gagaagatcc
     8161 aaataaaaaa gaaaaacttc actacatagc tatcggagat tcgctgactg aaggagtagg
     8221 agatcaaacc aagcaaggag gattcgttcc ccttgtcgca aatgatctaa aagaccgtta
     8281 taatttgacc gcggttgaaa cagaaaacta tggtgtaaat ggtgaacgaa gtgaccagat
     8341 cttaaagcga gtaaaaacaa aagaagaaat tcgaaacaat atcaaaacag ctgattttat
     8401 cacactgact gttggcggta atgatttgat gaaagttatc caaaataatt tattcgggtt
     8461 aacggtcaat tcctttaaaa agccactgaa aaactatcag gaaaatgtca cagaactatt
     8521 ggaagaattt cgatccctca ataaaaccgc acctatttac attttaggca tctacaatcc
     8581 attttatttg aactttcctg agatcactga catgcagaca attgttaaca attggaacaa
     8641 aggaacagaa acggtgacaa aaaaaatgcc gaactgctac tttatcccaa tcaatgattt
     8701 gctttatcaa ggattgaatc aggaagtagg tgtcacagaa gaaggaacag atcaaacaac
     8761 tagcacgaca gaaaatctaa atggcatcaa aaataatgtg ctctatgaag aagatcattt
     8821 ccatccaaat aatttaggtt atcaattgat ggcaaatgca gtgagagatc agctgatcga
     8881 gacacaaaac caatggttag agaaggagga tgatcaataa acatgaaaac aagatctgac
     8941 gtatcttcaa acaaaacaaa gaaaacaaat tactggaaat tcgccttcct tatccttctt
     9001 ggggtagtac ttggttcgac cgtattttta ggcagccgta tttttgctaa tcgggaacca
     9061 gaactcccag aaataccagc tcttacggaa agacaaggag atcctgtctt aacgatcaat
     9121 tcaaataaag aaaaagtcaa tcaaatcatc agctttttcc ttagtgagta tcaaaaagat
     9181 agtgatattg aatataaatt ttatttggaa aatgaagcac tactcaatgg tactttcgaa
     9241 gtactaggtt ttcctatcga tttctattta tattttgatc cgtatgtgat ggaaaacgga
     9301 aatgtccaac taaaagctag aagtctttct atcggaacat tgagtttgcc aatcaaagat
     9361 gtgatgaata tgatcaagag aaactataaa ttaccagatt ggatcgaagt caatacagat
     9421 gatctgacta tcatgcttag gttggatcaa ttccgaatgc aaaacgggat gtatattaaa
     9481 gcagataaaa tcgacttagt aaatgatgac attcggttta gtctatattt gccaaaagat
     9541 acctctgatg cttcaaacaa ctcaaatacc aacaaaacta gcaccagcag tactgaaagc
     9601 aagacaaaag aaaataaaaa atcatcggat gagtagtatt agagggggaa tcataatgga
     9661 acgagcaata tttgcaggag gctgcttctg gtgtatgatc cagcctttcg acacgcttcc
     9721 tggtatccat acagtgatgt caggatatac tggagggcat gtaccagatc cgacttatga
     9781 acaagtaaaa agccaaacga ctggacatac agaagcagtg gagattatct atgatccaac
     9841 attgatttca tatgaacaat tagtagaact ttactggcag caaactgatc caactgacgc
     9901 tttcggacaa ttcgaggatc gcggtgacaa ttatcgcccg gtgatctttt atttgacaga
     9961 agaacagcgg atcattgctg aaaaaagtaa gaaacagtta gctgaaagtg gacgtttcga
    10021 acagccaatc gttacaaaaa tcgaaccagc ccaaccattt tatctagcag aagaagacca
    10081 tcaagactat tataaaaagc atcctgaaaa ttttaaaaga aaccatgcac gccgggctgc
    10141 ctttatcgaa gaaaattgga ggaacgaagg gtgaatcgaa gcttttttca ttatttaatg
    10201 acactgcgag gcggtggcac acatgatgct ttatctgttt tcgctacaga agccggtaaa
    10261 gacatccaat ttcctaagca cagctctagc tacgaagaaa tttctaatta tctggaactg
    10321 aatgtcgact atttgccgtc catggatatc tttgacgaag catgggaaaa atatttagaa
    10381 aataatcatg acatataaga gatagttttg tactttatga t
//
LOCUS       DAJSKS010000097        10363 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJSKS010000097 DAJSKS010000000
VERSION     DAJSKS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.099"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            6..305
                     /locus_tag="ON396_002042"
     CDS             6..305
                     /locus_tag="ON396_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3114112.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ON396_002043"
     CDS             378..1739
                     /locus_tag="ON396_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR3114113.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ON396_002044"
     CDS             1761..2021
                     /locus_tag="ON396_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114114.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ON396_002045"
     CDS             2035..2787
                     /locus_tag="ON396_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HCR3114115.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ON396_002046"
     CDS             complement(2881..3486)
                     /locus_tag="ON396_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR3114116.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ON396_002047"
     CDS             complement(3539..4513)
                     /locus_tag="ON396_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCR3114117.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ON396_002048"
     CDS             complement(4891..5610)
                     /locus_tag="ON396_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3114118.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ON396_002049"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ON396_002049"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCR3114119.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ON396_002050"
     CDS             7161..8558
                     /locus_tag="ON396_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3114120.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ON396_002051"
     CDS             8639..9079
                     /locus_tag="ON396_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR3114121.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ON396_002052"
     CDS             9227..9553
                     /locus_tag="ON396_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3114122.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ON396_002053"
     CDS             9554..10303
                     /locus_tag="ON396_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114123.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3288 a         1828 c         2148 g         3099 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cac
//
LOCUS       DAJSKS010000098        10235 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJSKS010000098 DAJSKS010000000
VERSION     DAJSKS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.100"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            140..289
                     /locus_tag="ON396_002054"
     CDS             140..289
                     /locus_tag="ON396_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCR3114124.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            300..1814
                     /gene="dltA"
                     /locus_tag="ON396_002055"
     CDS             300..1814
                     /gene="dltA"
                     /locus_tag="ON396_002055"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCR3114125.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1811..3016
                     /gene="dltB"
                     /locus_tag="ON396_002056"
     CDS             1811..3016
                     /gene="dltB"
                     /locus_tag="ON396_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HCR3114126.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3061..3294
                     /gene="dltC"
                     /locus_tag="ON396_002057"
     CDS             3061..3294
                     /gene="dltC"
                     /locus_tag="ON396_002057"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HCR3114127.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3297..4565
                     /gene="dltD"
                     /locus_tag="ON396_002058"
     CDS             3297..4565
                     /gene="dltD"
                     /locus_tag="ON396_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCR3114128.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4706..5788
                     /locus_tag="ON396_002059"
     CDS             4706..5788
                     /locus_tag="ON396_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCR3114129.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5788..6591
                     /locus_tag="ON396_002060"
     CDS             5788..6591
                     /locus_tag="ON396_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCR3114130.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6661..7401
                     /locus_tag="ON396_002061"
     CDS             6661..7401
                     /locus_tag="ON396_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR3114131.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7412..8416
                     /locus_tag="ON396_002062"
     CDS             7412..8416
                     /locus_tag="ON396_002062"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCR3114132.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8461..10158)
                     /locus_tag="ON396_002063"
     CDS             complement(8461..10158)
                     /locus_tag="ON396_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114133.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3282 a         1696 c         2077 g         3180 t
ORIGIN      
        1 cagtgatttg atgtgctatt tttataaaag ctatgtgaca aaactgttag taaaaaaacg
       61 gtttctgtaa gctagacaca tgtttctttt tgtcaaaatt agttacactt tgtgtgaggt
      121 tatatgagga gggaatccaa tgaacaaatg gcgttctaat ccaaaattga attattgggg
      181 aaaattcatc attcgaacaa ttttttatag tgcagtactg ctgttcctga tttatctgta
      241 tcactataaa aatattcaag gaggaacttt tatctataac gaattttagg aggaagagta
      301 tggaaatcaa aacgattatt gaagcaatcg atgattgggg aataaatgaa cctgatcgag
      361 ttgcttatca ggcggatgag acgcatactt ttggtgaatt gaaacaagca tcagacgcac
      421 ttgcttatta tttagaaaaa aaggtagaag gcgatggacc aattgtcgtt tttggcaatt
      481 tggagtttga aatgattgtc tcatttctag gggttgtcaa atctggccat gcctatttgc
      541 cgattgaaga gcatacaccc aaggagcgta tcctatcgat ctttcgggtg gctaaaccat
      601 ctatggtcat cagtatcggg gactggatag aagatattcc ttcggttcct gtcataacaa
      661 aaaaagaatt tcaagaaatc aagaagatac cagtaggatt ttatcccaga aatagcgtaa
      721 aaggtacaga aaatttttat attattttta cttctggtac tactggagaa ccaaaaggcg
      781 tacaaatcag tcatgataat ctagtcagct ttgttcaatg gacgatggaa gactttggga
      841 tcaagcctgg tatgcatttc ttagcccaag cgccattttc ttttgattta tcggtatttt
      901 ctatttatcc tgcattagtt tctggcggaa tgctgaagcc gttaaataaa gcagttgttc
      961 aagactttcg tcaattattc gcgacattgc cagccttgaa gttgaatgtg tgggtttcta
     1021 ctccatcatt tatggatatt tgcttgatgg aacctacatt caatggagaa aatgtgccgg
     1081 aattggacat gtttttattt tgcggagaag aattgacgaa aaaaactgca gaaagtctac
     1141 ttgatcgatt cccaaatgca cggatatata atacttacgg tccaacagaa gcaactgtag
     1201 ctatttcaag tattcagatt gaccagcatg tactagatag ttatgaccga ctaccgattg
     1261 gctacgtaaa aaaagacacg aaagtttcta ttgtccaaga aggtaaacct gtttcaaaag
     1321 gagaaacggg tgaaatcatt attgcaggac caagcgtatc aaaaggttac ctgaacaatc
     1381 ccgaaaagac agctgctgcg ttcttttgtc aaaatgactt agcaagttac catacagggg
     1441 atgcaggtag actagatgaa tatgggcttt tattttatga aggacgtatg gatcagcagg
     1501 tcaaattaca tggataccgg attgaactcg gggatatcga acattatctg ctccaagaca
     1561 accgaatcaa acaagctgtc gtcgtaccaa aataccaagg aacaaaagta cagcagctgg
     1621 ttgcttttgt cgtattagaa aacaaaacga aacagccgga ttttcagttg actaaatcta
     1681 tcaaagaaca attactagaa gttgtaatgg attatatgat tcctcagaag ttccattatg
     1741 tcgatctgct gcctcagaca gttaatggaa aaatcgatcg aaaaaaatta acagctgagg
     1801 tgaatcctga atgatgttac ctcatatgat cccttatgca gagccgtttt atttttttct
     1861 tttagccatt gcgcttgtac cgatcgttct atctttattg atatgggaaa aaagattacc
     1921 agtttatcaa tcagcagtga cacttttctt tttatttgtc agctttggag gtaattattg
     1981 gcaacaggga gcggcgctga ttgggtatgt tttgtggcag acgatacttg tatggggcta
     2041 ttttgtttat cggcaaagga aaaatcagac aggatggttt tttgccgccg ttattttagc
     2101 gataattcca ctgtttttaa cgaaagtgac tccttttttt gaacatggaa aagcatcgtt
     2161 acttggtttt ttaggaatct cttatttaac ctttaaatct gtacaaatga tcatggaaat
     2221 aagagatggc atgatcaaaa catataagcc atttcgttat atccagtttc tgctgttctt
     2281 tccaacaatc tcttctggac caatcgatcg ctataggaga tttgaaaaag atcagctcca
     2341 tccgcctgtt cgtgataaat atgttgatct tttgggaaaa ggcgtccaaa atatttttgt
     2401 cggtttttta tataaattca ttatcggtta ttattttggt caagtactgc ttcctgtagt
     2461 gggacgcatt gccttacagc atggtggtat ttcatgggca ttagtaggtt atatgtacgt
     2521 gtacagtatg tatttgtttt ttgattttgc tggctatagc ttatttgcag tcggtacaag
     2581 ctacatgatg ggatatcaga caccaatcaa tttcaataaa ccttttttat catggaatat
     2641 caaagaattc tggaatcgtt ggcatatgac gctgtccttt tggttccgtg attatgtata
     2701 tatgcgattg atgttcttca tgatgaagaa aaaatttttt aaaagcaaga tcgtaatgtc
     2761 aaatatcggt tattttgctt tgtttcttct aatgggtata tggcatggat tgacgtggta
     2821 ctacatcgta tatggactgt atcatgcttg tctgatctgt ctgacagatg catggatccg
     2881 atttaagaag aagcataaag agcagttgcc ttctaacaaa tttacgcatg gcttagccgt
     2941 atttttgaca tttcaagcag tatgttttag cttcctgatt ttttcaggat ttttagataa
     3001 actttttttt agctaacgaa tagtgacata ttaatttata taacaaagga gaaaaggaaa
     3061 atggaagaac aaattttatc aatcttagaa gaaataacag gaacagatga agtaagaaaa
     3121 aatcgtgata ttgatctatt tgatgaaggg ttgcttgatt ctttagcatc tgttcaatta
     3181 ttagtcgagc tagacggaga gctggatatc caagtccctg tatctgaatt cgagcgagag
     3241 gattggtgca caccgaataa aatcattcaa caagcaactg cattgaaggg ttgagtatga
     3301 aaaagaaact ttttgctatt ttcggcccgg ttgttttagc tgttggctta ctgtttgtgt
     3361 tttttacctc ctcctatcaa gtagatatct ctaatccaga aacgattaga cgtgcctctt
     3421 cttccatgtc agtgaacgtg ttgaaaggag atgcgataaa aaattatgca ttatctgaaa
     3481 aacaatatat tccttttttt ggctcaagtg aattaagcag aatcagtcca tttcatccgt
     3541 ctgttctggc agaaaaatac cagcgaaact atcgtccatt tttgctggga gcacccggga
     3601 cacaatcgtt aagccaatat atgatgatgc gttctgcagg agatgctatg aaaaacaaga
     3661 aagtcgtctt tattatttct cctcagtggt tcgttaaaaa tggtgtaaaa actgattact
     3721 ttaatacgta ttattctgaa cttcaaacat acgactggtt gttttcaatg aagaaagtaa
     3781 cgcctgctga tcgctattta gctagaagat tattaacttt ctcaaaagta aaagagaatg
     3841 atacgttgac ggcgattctg caaacgatca aaaaagggaa gctaccttta cctgaatctt
     3901 tgaatcagct gcgcagccaa tggaacatgc tgaagagaga ggatgaagta ttcagcaata
     3961 ttgggttgaa agatcgacag caaaaaatcg atcatgagtc aaaacgatta ccaaaacaat
     4021 accaagaaac agaattatcc attttagcaa atcaaattgg agaacgagaa acgacgaata
     4081 atccattcgg gttgaaaaac gatttttata cgcatcgtat ccgagcccac gagccagagc
     4141 tcaaacagtc acaaaaaaat tgggattatc gtttttcgcc agaattttct gattttcagc
     4201 tggtcttgga tcaattagca aaaaaccata acgaagtatt gttcatcatc ccaccggtta
     4261 acgagaaatg gagcgattat accggactat ctcaagaaat gttacaagga tttgccaaaa
     4321 agatcaaatt tcaattaaat agccaaggat tcaatcgaat agctgatttt gtcaatcaag
     4381 cgggtaccaa ctattttatg gaagacacga tccatctagg ctggaaaggc tggttggctg
     4441 cagatcaaca gatcagaccg tttttagaag aaaatcatat tactgctagt aagtatcatc
     4501 ttgacgatgc tttttttagc aaatcttggc aacatcaaat accagataaa ctacaattaa
     4561 aataaagaaa acaggcgagt gttgctcgct tgttttcttt atttttgaat attctgaata
     4621 aaagataaaa ataccaagtt tgcctgaaca aaaatcataa gcaatggtat aatcacaata
     4681 tgaatataga aatgggggaa aacatttgga tacaaaagaa gaacagctac ttattgattt
     4741 aacttctgca aaaggagtac ctggaaatga agaagaggta agagaagtat tcagagaata
     4801 tgccaaacca ttcgctgatg atatttttta tgacggttta ggcagtgtga ttgccaaaca
     4861 tggtgctggc ggaggcccca aggtctttat ttctggacat atggacgaag ttggttttat
     4921 ggtaacaaaa ataaccgaaa aaggattctt ggaattccaa actcttggag gttggtgggg
     4981 gcaagtcatg cttgctcagc aagttgaaat caaaacacgt gaagggaaaa tcgtccacgg
     5041 tgtgattggt tccaaaccac cacatgtttt aacacctcaa gtaagaaata aaccttatga
     5101 aataaaggat atgttcattg atattggcgc atcaagccaa gaagaagcca aagagtgggg
     5161 catacgtcct ggagatatgg tcactccata tattgaatac aaacgaatga atggcagtaa
     5221 atatttactg gcaaaagcat gggataatcg tatcgggaca gctgtttctc tgcgagtatt
     5281 agagaatcta tctaaagaag cacacccaaa tgttttattc gcaggaagcg atgtgcaaga
     5341 agaagtaggc ttgcgaggtg ctcgaacaag tacacacctt gtaaatcccg atattgcttt
     5401 tgctttagat actggtacag caggagacac tccaggaatg acaccaaaag aagcagattc
     5461 gattttagga aaaggcccac agattttgat ttttgatgca tctatgatcc cgcataaaaa
     5521 attattgaat tttgttattg atgtagcaga ggaaatggat attccattcc aatatacagt
     5581 gatttctggc ggaggaactg atgcaggaca aatgcatttg acaagagatg gtgttccgtc
     5641 tgtagccatt accgttcctg ttcgttatct gcattcccat acatcagtga ttcatgaaga
     5701 tgattatttg aatacagtga aactagtcac agaattagtg aaacggcttg atagtgaaac
     5761 agttgaaaaa attcggagtt atgaataatg aataatagaa tacctatttc tgaatatcgg
     5821 aaaaaagcac gagtttcttt ggaaaatcaa tggggaatca atacttggct tgttttctta
     5881 tctataatat ttacgagcgt catccagaac ttttttgacg gtttcagtcc ttttccggaa
     5941 gaaagcatgc agcagcactt attgagtttt ctagtcgata atttactttt attcgcattc
     6001 aactatgcat tgttttacat tgcgctagta gttgtacggg gtggcagacc aaaaagtaac
     6061 cttttatttt cagttttcca aagaaagtat tttggttcga tacttggcat caatttgatc
     6121 aattcagtca tcaactggct actcaacagt ttaatctttc tgcccatgtt tttaatcagc
     6181 ggagtgagta cttatatgca gctgcttttc agttcaaatg cggtacctac gggtgtcatg
     6241 ccagaagatt ttcttgatat cagctttgtt ttatcgatga tagggatgat ttttgtttca
     6301 gcactcatca tgagtattgt tggcggttta ttccaatttg ctgcatggac aaagttcgac
     6361 tatcctgatt tgtcggttat tcaatgtttg aaatatggct ggtttttatt gaaagataga
     6421 attggtacgt atattttgtt gcagctgtct tttatcggct ggtatttgct aggagcaatc
     6481 gctttgttca tcggtctttt gtgggtcatt gtttatgtga atgtgtcagt tgcagaattt
     6541 tatgaacaag cacggattga aaaaggaggt ccagacgcct attttactta gcatgtaagc
     6601 agtttattca tcaaaaaaat aacttttgtg gtaggataaa tctttgaggg ggaatgtacg
     6661 atgaaattaa ctgttttagg atgcttagga gcgtatcctt ataaaaatca agggacaaca
     6721 ggttatttgc ttcaatctga cgggttcaat ttgttgatcg atgcaggtag tgctacgctg
     6781 atcaaattac aagaacacct tgatccactt gatctagatg ctgtgatcct tagccattat
     6841 catcatgatc atattgctga ccttggtgta ttacagtatt actggcaact tcatcctaag
     6901 agaaatccgg agacattgct ttcaatttat ggacacacaa aagattccat tcactttgaa
     6961 gaattgacgt tggaaaatgt ctcaaaagga catccttatt ttgaagctga agaattaaaa
     7021 attggtcctt ttctgattac ttttatggaa acaatccatc cagtagtatg ttacgccatg
     7081 cgtatcgtag aagaaacaac ggggaaagta ttcgtattta caggtgattc aggttatttg
     7141 gaaagtttcg ctgattttgc gaaagatgct gatttgtttc tagcagatac ctatcttttt
     7201 gaaggaaatg aacatcatca tgcccatttc acttcaaaag aagctggaga acttgcgaaa
     7261 tcagctaacg taaagaaact tgtacttaca cacctacctc agtttggtga tttagaacaa
     7321 ttgaaaaatg aagcgcaaaa ggcagcaggc aaagggattg atatagaact tgctgcagtt
     7381 gacaaagtgt ttaacattta ggaggaagaa catgattttt gtaccaaatg aaaaccacga
     7441 cccaagaatc aatttagcaa ttgaacaata tttgctgcaa gaaatgcctt tagatgaacc
     7501 tattttgctc ttttatatca atgaaccttc aatcatcatc ggcagaaacc aaaatacgct
     7561 tgaagaaatc aatcgagatt atgtggaaga aaatggcatt catgttgtcc gtcgcttaag
     7621 tggaggcgga gctgtctacc atgattttgg aaacctgaac tttagtttta tcatgcctga
     7681 tgatggggat tcattccgtg actttgcgaa agtaacacag ccaatcatcc aagcacttca
     7741 tgaattgggt gttgctgggg cggaattgaa aggccgaaat gatctagtca ttgatggcat
     7801 gaaattttct ggcaacgcga tgtatgctac caacggtcgg atgtttgctc atggaacaat
     7861 tatgttcgac tctgatatca atgaagtagt caatgcatta aaagtaaaaa aagacaaaat
     7921 cgaatcaaaa ggaatcaaat cgatccgttc tcgtgtaacg aatatcaaac catttcttcc
     7981 agaagaaaag caaaaaatga caacagaaga atttcgtcaa gaaatcttgt tgaaaatttt
     8041 cggcgtagag tcagtagaag aagtaaaaac gtacgaattg actgatgaag attggaaaaa
     8101 aatcaatcga atctctgagg aatattatcg aaattgggat tggaattacg ggaagtctcc
     8161 agatttcaat ttctctcgcc aaaaacgttt ttctatcggt tcgattgaag tccatttgaa
     8221 tgtctcagaa ggaatgatca aagaagcaaa aatctttggc gatttcttcg gactaggaga
     8281 tatctctgac gtggaaaaaa aattggttgg acagaaatat gataaagcat ctctatcaaa
     8341 aatagtcagc caaattgatg tgaagaaata ttttggtgcg atagaaccag aagaattgct
     8401 taccttactt tattgaaaat aaaaaatagc ggaatccaca tatatggatt ccgctatttt
     8461 ttatacagga ttcgggtaat ttcgagagag ttgaacagtg atccgatcaa tgaaagtatc
     8521 tttttcctgc gttagtgttc ctgtgtaacg atccgctacg tctttcatca tcattaagtc
     8581 aaaacgttcc tgatcatcta attcttctgt gatgcctcga ctaaataggt ttgtatcgta
     8641 atgtgcacta tgttcgcaac aaatgacgaa atgttcctct tgttcgtgta tcgacaaata
     8701 aatttttcga tacagtggat tttcgatggc atgagtttct ctaaaggaat gctctaaagc
     8761 gtggatcaaa agttgagcca gatcgtcatc gttgatagga agttcttcgg gaagatctgc
     8821 ttggaaggtt atctgaatat tccgtttggc agctaacttc tggtaacgcg ctaagataag
     8881 atttgtcacc tggtggttgc aaaaagaaca cagcatttcg aaattttttg cttcttctaa
     8941 tatcttttcc acatagtttt tagcttttgt ttggtccgta tgcattaaat cttgcagatt
     9001 ctccatctct tggacaaatt ccaggcgaag tgtatttact tcttggatat gctgtcgaag
     9061 ctgctcataa tgttgctcca acaaatcatt tttgattttt aagaaatcta cttgtctccg
     9121 atctttgtcg gtatttaaga tgtgatcgat tagattgtag ctgataataa cgattagaat
     9181 gataatatat aaaatcgtag accaattgat cgttgtttga tagataccta atgccgtttg
     9241 gatgtaaatc aagcaatgga taatggctac caagacgatc caaggtgtac aagctacata
     9301 aaaacggtta ttcttatatg cttcgccaat gcatgcaagt gaagtgacta aagtgcttat
     9361 aacactagca atattagcaa tcggcattaa ttcaggaagt tctgcaggag taaaagttgc
     9421 ataaagcaga acgccgataa aaatcaaaag ttggaacgta caaaaaattc cgtaccattt
     9481 tttatagtaa aacattcggc tccaatagta gcagatgatg aaagctgacg ttaagataat
     9541 aaataaatga gataaaaaag aatgaacgaa tggttcaaaa agaataccac ttaaaatatc
     9601 ttctgataca gcttcaaatc ctaatgccaa agcaaagcaa cttaagagaa taagtgagta
     9661 ttcaagacgt ttttgtttat acatcaagta gatcgtatag aaaaaggtcc ctatagcgat
     9721 aaaaacagcg attatcatcg tcaatacttg cggcactgat tgtgaataaa taaaactaat
     9781 caatggtcca gttgttccta gataaacttt cggtggcagt ccagcatagt tctgataagg
     9841 agatgagact gtaagggtca atttttttcc gatataattt gctggtaaat ctatcacagc
     9901 caatgagagt ccgggattgc tttcttcctg ataatctttc cgttggtata aaatcttttc
     9961 gcctagatat acagtaatcc attgatggtt tcctttgatt agtaaatttg cttcgtccaa
    10021 cggtttttca agtgtcgttt ccataatgaa tgtttcgtct ttatccacgg taggaatcgt
    10081 acgagtgtac ttgctgtcaa acttaaaatc aggatcatct tttgtataga aaacccacga
    10141 atcttttatt tctttcattt gattgctgtc cgcaggagaa aaaataaaat aaaagaaata
    10201 agcaattcct gttaaaaata acaatgataa tataa
//
LOCUS       DAJSKS010000099        10168 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJSKS010000099 DAJSKS010000000
VERSION     DAJSKS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.101"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(47..367)
                     /locus_tag="ON396_002064"
     CDS             complement(47..367)
                     /locus_tag="ON396_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCR3114134.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(515..2749)
                     /locus_tag="ON396_002065"
     CDS             complement(515..2749)
                     /locus_tag="ON396_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3114135.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            3228..3494
                     /locus_tag="ON396_002066"
     CDS             3228..3494
                     /locus_tag="ON396_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCR3114136.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            3494..5221
                     /gene="ptsP"
                     /locus_tag="ON396_002067"
     CDS             3494..5221
                     /gene="ptsP"
                     /locus_tag="ON396_002067"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR3114137.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            5385..6431
                     /locus_tag="ON396_002068"
     CDS             5385..6431
                     /locus_tag="ON396_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR3114138.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            6448..7671
                     /locus_tag="ON396_002069"
     CDS             6448..7671
                     /locus_tag="ON396_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR3114139.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            7760..8647
                     /locus_tag="ON396_002070"
     CDS             7760..8647
                     /locus_tag="ON396_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR3114140.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            8733..8963
                     /locus_tag="ON396_002071"
     CDS             8733..8963
                     /locus_tag="ON396_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HCR3114141.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(9020..9985)
                     /locus_tag="ON396_002072"
     CDS             complement(9020..9985)
                     /locus_tag="ON396_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR3114142.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         3243 a         1789 c         2050 g         3086 t
ORIGIN      
        1 ctttactttt gtcccacgct ctttaattat tatgttcaaa atttgtttac acggctaatg
       61 tagcgcctaa ttcatgtttt ggaatcgaga tttcaacaag gcctgccaat tcgattactt
      121 tgatttcatc ctcgtggtaa acggcgtctt tgattttatc taagaaataa tttttagcat
      181 cttcgatttc tttcggctgg attttacgtt tgtcattgat cagcagaatt tcattatgtt
      241 gaggggcttc agtgtagaca atagtggtat ttccggctgt gtagaccttg actagatggg
      301 catcagtgtt ttcaagctgg tttaatacaa gacgggaatg gctgtttgtt acatttacca
      361 atttcatggc gctcacctct tttatgtatt tttaattgct tgtttctctt attataattt
      421 cttttttata aaatgaacag tattttgact tttctgaaaa aagaatgaga cggcatttct
      481 caaagaaata ccgtctcacg ctataaaact ggatttattc agccgtttct tcggctgatt
      541 ctttagccaa acgttctggt ttagaagtaa cgatgatttt tccatcttta tccagttttg
      601 ctttcaactc atggatagaa gggtgatcaa gataaaattc agcgatacca tcttcgattt
      661 gttcttggat cgtgcgtcga agaggacgag cacccataga tggatcatag cctaagtcaa
      721 ctaatttttc tttgacattc gtaggaactt cgatgtgaag ttgctgtgct gccagcattt
      781 ggttgacatc gtctagcatt agtgtgacga ttgtcatcaa gttttctttt gataatgcac
      841 tgaattcgat gatcccgtca aaacggttca gaaattctgg tgtaaagtag ttgtttaatt
      901 ggttcaatac agaacgagta acaccttcgc gtgccgcacc aaaaccaacg tttgcttcta
      961 ctttaccagt tccggcatta cttgtcataa tgatgatcgt atctttaaag ctgaccgttc
     1021 ttccttgtgc atctgtcaat cgaccatcat ctaagatttg caagaacata tgcaaaacat
     1081 ctggatgagc tttttctact tcatctaaca agacaagact gtaaggattt ctccgtactt
     1141 tttctgttaa ttgaccagct tcttcgtatc cgacataacc tggaggagaa ccaatcaatt
     1201 tagagacact atgtttttcc atatattcag acatatcgaa acgaatcatt gaatcttgtg
     1261 aaccgaatag ttcgtaggca agttgtttcg caagctctgt tttacctaca ccagttggtc
     1321 caacaaataa gaaggagccg attggacgat tctgtttgtt taatccaaca cggtttcggc
     1381 gaatcgcttt cgctactcga tctacagcgt tatcttgtcc gatgacatga gctttcaagt
     1441 catctgctaa gtttttcagt tgtgtctgtt ctttttcttt taattcaccc actggaatac
     1501 ctgtccgttg ttcaacgatt ttttccatat ctttttcggt aatcacaggt gtttcctctt
     1561 cagtgagctg acgttctttc attttttgaa gtttattgat ctgatcgcga taataagctg
     1621 ctttttcgaa atcttcttct cgagaagcta aaactttttg ttcttctgct tcttgcagtt
     1681 ttttctcgat tgttttagga tcaacgattt ggatcgtcaa gtttttctta gaacctgttt
     1741 catctagcaa atcgatggct ttatccggca agaagcgatc ttggacgtaa cgattggata
     1801 aagttgcggc tgcttcgatg gcctcatcag tatattttac atgatgatag tcttcataac
     1861 gtttttgcaa acctttcaaa atactgatcg tttcttcgac agttggttca tctacccgta
     1921 caggttgcat acgacgttct aaagcagcgt ctttttcgat gatgcggtac tcatttaagg
     1981 tagtagcacc aaccatttgc aattctcctc gagctaaagc tggttttaaa atattaccag
     2041 catccatatt tccatcacca gcagaaccag caccgactat ttcgtgaact tcatctatga
     2101 ataggatcac attttctgct tgacggatct cttcaatcaa tttttgcata cgttcttcaa
     2161 attgtcctcg tataccagta ccttggacta aagatacgac atctaaacga atgacttctt
     2221 tgtccattaa tttttgcggt acatcaccat ctacgatttt ttgtgctaaa ccttcaacaa
     2281 ctgctgtttt accaacacct ggttctccaa tcaagacagg attgttcttg gttcggcgat
     2341 ttaaaatttc aatgactcgt ttgatttcgt catcgcgtcc gatcacagga tcgatatcac
     2401 cttgtcgtgc ttgttctgtg atattgattc cgtattctcc caataaacct tctgtattgc
     2461 cttgaggtgg ctgaccacca ttgaagttgt tgtttccgcc aaattgtgtt ggtggtgttt
     2521 gctcataatt tccttgctgt tgcatttggc gagacatgga acggaaaaga tcgtccaaac
     2581 tgccaaatcc aaatggatca ttttgggcca tattagtcag acctccattt tgttgatttt
     2641 tgattttttg ataacagctc tgacagtaat cgagttgtgt ccgctgacca ttgacagtgg
     2701 catacaagtg aatcgttgcc tcattcttgc cgcagttttg acaaagcatg aatcattcac
     2761 gtcctttcac aaaaatgggt acatccatat tttacatctc gtttttcatg aggtaaaaga
     2821 ataaccccta gttttttaag agatttatga ggtagaaaag gtgaaatctc tattgaccat
     2881 tcctgacctt tgactcttat tatactaaaa ataaaaaggg aagcaagtaa ttcgtttcat
     2941 atgaaaacaa agagggtcaa aggaaagaat gataaacgtc aggagaagag aaagcgacaa
     3001 gaaaaaggaa ataaacacgt ttgttataaa atgtgaagaa aaggatgaaa aagaatccgg
     3061 aataatttga atacttttat ctgaaattga atgaaaactg ttgtatctaa cgaaaaaaaa
     3121 tgttaatctt tacagatgaa agggtggcat ttgttaggat tctctgaatt actaaaaaag
     3181 aaaaccctca cataaaaaca acactcactt aaaggagacc gattaatatg gaaaagaaag
     3241 aatttcacgt agtagcagaa actgggatcc acgctcgtcc agctacacta ttagtacaaa
     3301 cagcaagcaa atttaactct gatgtaaact tagagtataa aggtaaatca gtaaacttga
     3361 aatcaatcat gggcgttatg tctttaggtg taggtcaagg ttctgatgtt actattactg
     3421 cagaaggtgc tgacgaagct gatgcaatgg cagcaatcgt tgaaacaatg aagaaagaag
     3481 gcttatctga ataatggttg aaatgctaaa agggattgcc gctagtgacg gtgtagccgt
     3541 tgcaaaagct tacctgctag ttcaaccgga tttaacattc agtaaggcaa cagtagaaga
     3601 tactgcggct gaagaagctc gtttggatgc tgcattagct aaatctactg aagaattgca
     3661 gcaaattcgt gaaaaagcag cgcaaagctt aggtgaagca gaagcgcaag tatttgatgc
     3721 ccatttgatg gttctttcag atccagaaat ggtaggtcaa atcaagcaaa atattaaaga
     3781 caacagcgta aatgctgaat cagcgctaaa agaagtaacg gatatgtata tcggtatgtt
     3841 cgaagcaatg gaagataatg cttatatgca agagcgtgct gcagatattc gtgacgttgc
     3901 taaacgtatt ttagcgcact tattaggcgt tactttacca aatccatcaa tgatcaacga
     3961 agaagtggtc gttgttgcac atgacttgac tccaagtgat acagcacaat tagatcgcaa
     4021 ttttgtaaaa gcgtttgtaa cagacattgg tggacgtact tcacattctg ctatcatggc
     4081 acgttcttta gaaattccag caatcgttgg gacaaaagaa attactgcta aagtcaaaga
     4141 aggcgatatt ttggctgtca acgggatcga aggcgatgta atcatcgatc ctacagatga
     4201 acaaaaagct gaatttgaaa aagcaggtgc agattacgca gcacaaaaag cagaatggga
     4261 aaaattaaaa aatgcagaaa ctgtcacagc tgacggcaag cattttgaat tagctgcaaa
     4321 catcggaaca ccaaaagatc tagttggtgt ccataataat ggtggagaag cagttggtct
     4381 gtaccgtact gaattcctgt atatggattc tccagacttc ccaacagaag atgaccaata
     4441 tgaagcttac aaagctgtat tagaaggtat ggaaggaaaa cctgtagttg ttcgtacaat
     4501 ggacatcggt ggagataaag aactaccata tctgcaatta cctcatgaaa tgaatccatt
     4561 cttaggatat cgtgcattgc gtatcagttt atctgaacaa ggcgacgaaa tgttccgtac
     4621 acaaatgcgt gcattgcttc gtgcatctgt tcatggtaat ttacgtatca tgttccctat
     4681 ggtagctaca ctcaaagaat tccgcgcagc aaaagcgatc tttgaagaag aaaaacaaaa
     4741 attgataagt gaaggcaagg aagtatctga tacgatccaa gtcggcatca tgatcgagat
     4801 tccagcagca gcagtcttag cagacaagtt tgcaaaagaa gtagatttct tctctgtagg
     4861 tacgaacgat ttgattcaat acacaatggc agcagaccgt atgaacgaac gcgtttctta
     4921 cttgtaccaa ccatataacc catcaatttt acgtttgatc aaaaatgtaa ttgatgcagc
     4981 acacgctgaa ggaaaatggg caggtatgtg tggagaaatg gctggagatc aaacagctgt
     5041 tccattgcta gtaggtatgg gtctagatga gttctcaatg agtgcgacat ctattcttaa
     5101 aacgcgtagt ttaatgaaac gcttggacac agcgaaaatg gctgaacttg ctgatcgtgc
     5161 attgaaagaa tgcgatacga tggaagaagt cgttgaactt gttcatgaat atgtaaaata
     5221 aacaacgtat caaaagctgg tgtggcttgt aaaagagcta cgccagtttt tttgttcttt
     5281 tttaagaaaa cgtttttgtc agtaagaata gacaaaaata gggcttaagt ttgagtgaca
     5341 gtttgtaggc tatgttatac tatataagtg aagggggacg taaggtgaag gtattattat
     5401 attttgaaag tgaaaagatg cttgcaaaat ctggaatcgg gcgtgcgctt gatcatcaaa
     5461 aacgtgctct gacggaagtc ggtatctctt atacactaga tgaaaaagaa gattatgaca
     5521 tcctgcacat caatacatac ggaatcaata gtcataatat ggtcaataaa gccagaagag
     5581 aaggaaaaaa agttgtctat catgcgcatt ctacagagga agatttccga aattccttta
     5641 tcggatcgaa ccagctttct cctatcgtaa aaaaatatct ggtaggattg taccaaaaag
     5701 cggactatct gatcacgcca acaccttatt caaaacagtt gttggaaagc tatggtatcc
     5761 gtgtgccgat tcaagctatt tccaatggga tcgacttaga gaaatatcac cctgatccaa
     5821 tgaaagaaca aaagttcaga gaatatttca aactgtctcc ggatcaaaaa gtaatcattt
     5881 gtgtcggttt gtttttcgaa cgtaaaggaa tcattgattt cgtagaaatt gctaagaaga
     5941 tgcccgaata cacatttatt tggtttggtc atgttcccat gtactcgatt cctcggaaca
     6001 tccggaaaat tgtaaaagaa gatcatcctg aaaatgtcct gtttcctggt tacattcgtg
     6061 gagagatcat agaaggtgcg tattcaggtg cagatctatt ctttttccct tcttatgaag
     6121 agacagaagg gatcgttgtt ttagaagctt tagcaagcaa acaaaacgta cttgttcgag
     6181 atattccagt gtataatggc tggttagaag atagcaagaa ctgctatatg gggaagacaa
     6241 acgaagaatt tatccgactg atccaacaga ttaccaatca agaactaccg agtacgacac
     6301 aagccggtta tcaaacagca aaagagcgaa gtatcaataa aatcggtgaa gaacttaaaa
     6361 atgtatacga aagtgtactg aatgaaaaag tctctagcaa attaaaaaaa gtaaatcagg
     6421 tcaaggacta gtttggaggc gaaaaacatg aaaatcggct tttttaccga tacctacttt
     6481 ccgcaagtca gcggagttgc tacatcgatc aaaacattaa aacaagaatt agaaagacat
     6541 gggcatgaag tctatatttt tacaacgacc gatcctaatg cggataagtt ggaaaaagat
     6601 gtgattcgaa tgccaagtgt accatttatt tctttcaagg atcgaagagt tgttgttaga
     6661 ggcatgtggt atgcttattt ggttgccaaa gaactagaac tagatcttat tcatacccat
     6721 acagaattcg gagcagggat acttgggaag atggtagcta agaagttgaa aatacctctg
     6781 atccatactt accatacgat gtatgaagac taccttcatt atatcggtaa aggaaaagtg
     6841 atcaggcaat cccatgtaaa atacttgtct agattatttg caaatcatac gacaggtgta
     6901 gtttgtccaa gtgaacgtgt tattgataca ttacgcagct atggtgtaat agcaccgctt
     6961 cgcgtgattc ctactggtat cgacattgaa aaattcaaac gttctgatat cactcagcag
     7021 gatatcagca aattaaggga gtcactaggt cttcaggaaa accatttgat gattctttct
     7081 ttaagcagga tttcttatga gaaaaatatc caagccttga ttaatgggtt tcctcaggtc
     7141 atctccgctt atcctaacgc cagattagtt atcgtaggaa aaggacctta tgtagaagag
     7201 ttagaggaat tgatctcaga actgcagtta gaagaatttg tgcagttcac aggagaagtg
     7261 gacaacaatg atgtagcact ttattataaa gcggcagatt attttgtcag tgcttctact
     7321 tcagaaacac aagggcttac ttatacagaa gcgatggcag ccggaacacc ttgtgtcgtg
     7381 gaaggaaatg cttatttgaa ccgattgttc gatcatcctt cattagggaa aacttttgaa
     7441 accgacgaag actttgcacc agcacttatt gattatattt ccagtgaagt tcctaaagat
     7501 ccaaaagttt tagaagctaa actttatgat atttcatcta cacatttcgg cgaagctatg
     7561 atcgaatttt atcaagacat ggttgtttat catgaagaag agatgcgaag aaaagaagaa
     7621 gaagactcga ttgaacgaat caaaataaaa ttaaattcat ttaaaagata aaattcaaac
     7681 attttttatg aaaaaatcct tgtttctagc gtatactagc atagaagcaa ggatttttga
     7741 tttttaggag gtcgaaaaaa tgaaacttgg ttttatcgga acaggcgtca tgggaagtgc
     7801 tgtcgcacgc catctactag aagcaggaca cgaagtagct gtctataatc ggacaaaagc
     7861 aaaagctgat cctttagtaa cagaaggagc tatctgggca gatacaccaa aagcggtagc
     7921 agaacaaagt aatattctct ttacaatggt aggttacccc aaagatgtag aagaaatcta
     7981 ctatggacag tcggggattt tttctgcaga tatctcagga catattttag ttgatttgac
     8041 tacaagtaca ccaagcctgg cagaaaaaat tgcaaaaaca gcaaaagaaa aaggcgcaga
     8101 tgcgttagat gcaccggtat ccggtggtga cttaggagca aagaatggta cactgacgat
     8161 catggtcggt ggagaagaag ccgtttatga tcaagtactg cctttattca aagaatttgg
     8221 tacgacattt acattgcatg gttctgctgg gaaaggtcag catacaaaaa tggctaatca
     8281 gatcatgatt gcaggtacga tgacaggaat gacggaaatg cttgtctatg cacaaaaaaa
     8341 cggacttgat ttaaagaaag tgattgagac gctttcaggt ggaagtgcag ctaactggtc
     8401 tttaagcaat tattctcctc gtattttaaa agaagattac acacctggtt tttttgtcaa
     8461 acattttatc aaagatttaa agattgcatt agaagaagca gaaaaaatgg atcttgtatt
     8521 gccagcaact acccaagcat tgaaactata tgaagagttg gcagataaag gttttgagaa
     8581 tgacggaacc caagctttga tcaaactttg gtggcctgaa ggaaaaatac cagaaaaaaa
     8641 atcataaaaa acgagttttt ttagagaaaa tcaatgcaag taggctatgc ttttgataca
     8701 atataacaga aataagaaaa ggaggacctc cgatgacaca ttcacaatta gtcgcaatca
     8761 taaaacgact ggaggctatg gtcgagtcag cagataatga actgcaagtt cgccgttttg
     8821 aaaaagaagg cgtagagaag tgtacagtaa cttatgataa agcgaccgat acatttgaat
     8881 taactgaaac agatactcat cagcaatacg aatttgataa tatcgatatc gtagcgatgg
     8941 aaatctatga cttaatccaa taaattttag gataattgac atttgaagtt gaagatgcct
     9001 aaaagaagcc gagaagcaac taatttagtt gcttttcagc ttcttttttg tttaacagtt
     9061 caatttcaaa ctgttctttt tctaattttt tgcggtaact gatcaagatt tgtacaggat
     9121 cgatttcttc ttttgacaat tgacgtttca aagataaatg agcttctttc ttttcataat
     9181 agaagtcagg agaaacgtca ccagaggtaa tcattgtttc tgcaagttga ccataacaaa
     9241 cgaggtagtg ctttttctga ctctcatctg ctgctaatgg catgctgaag gtgagcgatg
     9301 gtgttcttaa aaactgcttt tgttcctgga tccgtctaat aaaataagta actaattcga
     9361 tatctttttg aagcataccg gctttttcga aattcaactg atagatgaaa tgttgttgtc
     9421 tttttttcaa gtaagtcagt agctttttat ttcggccttg aaacagccct tggagttcct
     9481 tttttttctg ttcaaaagtc atttcctgta ggtcaggaag ctggactcgc aaagctaaca
     9541 aactaatttc agacaaccaa ggcatttgat aagtttcttc cattatacgc aaaatagacg
     9601 gcattttttt atagagacga aaaggcccgt atgttcgagc cgtaggagta tctgttaata
     9661 caaatccctt attggtaata tgtacataat tataattact gaaataattc atttgtcggt
     9721 tataaaaagg acggtaatgt tggatcaatt gacactcgag caataaagca tctaattccg
     9781 tgtctactac gacgaaatca aaatctgtta tatgatgaat catacgtaat actttttttg
     9841 aatgctgttt attctgatga aaatagctgc ttactctatt tttgagccgt tttgctttac
     9901 caacatagat gaccttacca tctcggtctt tcataagata gacacccggc agcatgggga
     9961 gagttttcat tttttctttt aacataggat ctccttttac gctttgaata aaaagactat
    10021 aacaaatttt gaaataatgg acaagtttta gggaattcta tagttctgtt cacagaaaat
    10081 actttgataa tcaaaatatg tgagatagat gatttttcta ataaaagaaa ttataaatgc
    10141 tgatttatca gttgttttaa atgagtaa
//
LOCUS       DAJSKS010000100        10086 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJSKS010000100 DAJSKS010000000
VERSION     DAJSKS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.102"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(24..1043)
                     /locus_tag="ON396_002073"
     CDS             complement(24..1043)
                     /locus_tag="ON396_002073"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR3114143.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(1676..2680)
                     /locus_tag="ON396_002074"
     CDS             complement(1676..2680)
                     /locus_tag="ON396_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3114144.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(2693..3742)
                     /locus_tag="ON396_002075"
     CDS             complement(2693..3742)
                     /locus_tag="ON396_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3114145.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFFFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(3759..4580)
                     /locus_tag="ON396_002076"
     CDS             complement(3759..4580)
                     /locus_tag="ON396_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3114146.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(4567..5346)
                     /locus_tag="ON396_002077"
     CDS             complement(4567..5346)
                     /locus_tag="ON396_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3114147.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(5363..5833)
                     /locus_tag="ON396_002078"
     CDS             complement(5363..5833)
                     /locus_tag="ON396_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3114148.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(5845..6264)
                     /locus_tag="ON396_002079"
     CDS             complement(5845..6264)
                     /locus_tag="ON396_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HCR3114149.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(6345..9122)
                     /locus_tag="ON396_002080"
     CDS             complement(6345..9122)
                     /locus_tag="ON396_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3114150.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            9313..9537
                     /locus_tag="ON396_002081"
     CDS             9313..9537
                     /locus_tag="ON396_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114151.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         2944 a         2100 c         1658 g         3384 t
ORIGIN      
        1 acgattaact attgatcatc tatttaacga agagaaaatt cattctccat ttttccgtca
       61 cgtaacatga tttcttttgc tgcttttttg atatcttctc cattatacaa aacattatag
      121 attgtttgag taattggcat ctcaacacct agctgttgag ctagctctac tgctgctttt
      181 gtcgttgcca caccctcaac gaccattccc atgttttcta gaacttcttc cagcttatgt
      241 ccctgtccta acaagttacc ggcacgccaa ttgcgagaat gaacacttgt acaagtaacg
      301 atcaagtccc ccacaccact caaaccgata aatgtcagag gattggcacc catcgcgaca
      361 ccaagacggc tgatttctgc caatcctcga gtcatgattg ctgcttttgc atcatctccg
      421 aatcctaatc catggatagc gccggcacca attgcaatga tatttttcag tgctgcacca
      481 gtttctacgc caatcacatc attatttgta taaatcctga aataatcatt catgaacagt
      541 ttttgtacat aggctgctgc tttttgatca atacttgcag ctgtaattgt tgtgatatca
      601 tgtactgcca cttcctcagc atggctagga ccagataata caacaattgc ctgtcttttt
      661 tcttctggaa tttcttcagc caaaacttct gagattcgtt tatgcgtatc ttgttctagt
      721 cctttacttg catgtataat gattggctta gtattcattt taccaataag ttcctgtgcg
      781 actgaacgaa tcgcttttgt tggaacaaca aataaaatag catctgcgtt atccactgct
      841 tctgccaatt ctttatatcc gataatcgat tctgggatcg tcaaatcagg taaaaaatgc
      901 tgatttgtat gatacttatt gatttcatca atctgttcag gtacatttcc ccaaatacgg
      961 acatcatgcc cgttttctgc caatgtttgt gctaaagcag tgccccaaga tccaggacca
     1021 agaactgcga ttttttgttt catggatatt cctccaaata aataattgat aatttccatt
     1081 atagtataca tttcttaaag ttgggactaa tttccattgt ttttaagtgt ttgtttactt
     1141 cttttatcgt ttttacttga aataatcaat gatatgttac aatagtctac attttatttt
     1201 tacactcgtt atgctttgct tgttcctaag aggaagcagt tttgacaatg ttactaccag
     1261 cattactatt aggaggttag gaaacttctc ctagtgagta ttgatttaaa atacataaga
     1321 gacagattga acatttctaa atctgtcagg tcctgcaacg gacagattgt ctctgttgac
     1381 ggttgacata ggaacttttg ttttagtcaa cattctggat acaaaacgct atgtaaactg
     1441 gcgcaactcg ttgtacttgg acttttacta cggatccatg caacaactaa atagtactat
     1501 tgcataacaa aaaatggttg gcacccacat gtcccattaa acagattata agttgaactg
     1561 tagataatgc aaaaaatgtg ttttataacg taattgatgc caatatttta aaaaaataaa
     1621 gggagcagtt atcgctgctc tctttatttt ttatattctt tttatccagt ctatcctaat
     1681 aatcaaactg acgatagtag cggcgtatct ctaatggatg gcagttcaac tgctccacat
     1741 gagcatcgat gcgttgtgtc actacatgca tcaataagaa agacaaaaca cgtccgcgat
     1801 atttttcact gataccttct actggatagt cttttgtgtc tatcaatgta taattgctgc
     1861 aaatttttgg caaaagttta gccacacgtt cagataacac tctctgttca tcttcgccta
     1921 aaaataatgt gactggggtt gtttcttcta cgatctctaa cgtcccgtga agaaactcgg
     1981 cagcatggat agatttcgat ggaagccagc tttgttcttc ccagtaacac atggcatatg
     2041 aataaacagc tccccattga ttccctgctc caataaaata atgcatacta tcacgtcgat
     2101 gtttttctgc aaatgataag ccaaatgcat ctgctttttt ctctgcttcc actaaccctg
     2161 ttggtaaata acgttcgagc tgttcatagt attctgaata agcttcgaat tctccattca
     2221 aatacatcaa tcgatctgct gccataaaaa atttgatttg ttctgttcca ggagctggat
     2281 aagtgaccac aaaatcgcat agctgagaca acttactgtt cgctttatcg ataaatccaa
     2341 tcagtgtcgc tcctactttc ttgatttgtt ctactgcttt cactacttct tgagttgtac
     2401 cggtaacaga ggacaaaata acgatagaat cttttgttaa tcttttgttt ccagtagtgt
     2461 aatattctgc agcatgctgt acaaagacag gcaagttgct tttcccattc atataagtga
     2521 cagcttgcat cgaggaagca tacgttccac cgattcctag ataatagaca gcgtcaaatt
     2581 tttccgcata aagtttatcg atgatttctt ctacttgcgg acgtaaagct agtgcgcctt
     2641 ttattgcttt gatttgttct tcttcattga atttcagcat gatttccacc tcttataatt
     2701 tacttttcaa gactgtatca aagtcttcaa aaatcttttt gttcaagtca atacctttag
     2761 ctactgcggt tttataagcg aatagttgaa acggaacgac aagcgctaat acagacagat
     2821 attcatcaca ttctattgct aatccaagtg ttttctcgtt tcttgctttt tttgtggtaa
     2881 tggtgagagt ctctccaacg taccgactca tatatctttg caacttttgc gaacgttcct
     2941 tatttacact ttccccttca ataaagaata gcaaatgcga agcatctgcc tccaggtatg
     3001 ggccgtgcat ataagcttct aactcaaatc cttgtgacgg aacgcggatt gtttcagtca
     3061 atttcgtctc tgcttcttta gctgttcccc agtttggacc atatccaatg gtgacgaaac
     3121 gctgtgctaa acgaaataaa ctttgatatc catcaaaaaa ttcttccgtt ttttgaataa
     3181 tcgctggtaa atggttgatt attttgcgca actgttgcat gtagtcttgt tctatttttt
     3241 tgctgatttt atttttactt attccaattc ctatccctaa gagtagtaac tgtaaaacag
     3301 tcgcagaata gccttttgta acgaagccta ctgattctac cccggcacct aaatcaataa
     3361 tttgtgcagc ttctaaagca agtggacttt gcacgtcatt tgtgatagca acaacaggta
     3421 acccttgttt ttgtatcatt tttactgctt caacagtaga agcacttttc ccgctttgtg
     3481 acacagcaat cacagtatcg attgaaggag aaagttttcc ataattgaaa aaaaaaaaag
     3541 gttcttgaat atcgacagta agatcaccat aagattccaa tgctggcttc gctgctaaac
     3601 aagcgttata agatgaaccc gttgctaaga tcagtaaatt cactgttttt ttcatatgtt
     3661 ctcttgtcga aaaatcattc tttttcaaga tcgtacttag tacttccttt tcttcataga
     3721 tataatcttg catcgtaaac attttctctg ctcctttttt aaaaaatccc tatccacgaa
     3781 ccaaaaattc caaagaaagc tagtccgatc aagatcgtta atggtttcac ttctttcttc
     3841 aatagataaa acactatgcc aaatgctcca agacataaga tatttggaac gatatcatca
     3901 aaaatacttt gaacagtcac agcattttct cctgaaccga tttttaaagg aatattgatg
     3961 gtgatcatcg tggcagtcat tgctccaacg accatcagtc caataataga cgcgcctaat
     4021 gtcaaacttt ccatcatacc gtttgcctgg atctttttca aaaaacccgt tcctaagcga
     4081 tatccccaac ctgttgctag atagcgaaac agaatgtgcg gaatattgaa aatcaaaatg
     4141 aacaagatag ggcctaaaat atttccttgc atggctaaag aggtacccac acctgtggca
     4201 atcaatcgca aagttcccca aaagaaagaa tctcctaacc ctgcaagagg tcccatcaaa
     4261 gatgttttga tactatcgat cgcagtcaca tcaaattcag gatcatcctt attttcttcc
     4321 tccattgcag ccgtgattcc taaaatcagt gtaacgatat gtggggtcgt gttaaaaaac
     4381 tctagatgcc gtttcaaagc tgctgccatt tcttctttcg actgatataa ctttttcaaa
     4441 atcggaatca ttgcataaac ataagccata ttcatctgac gttcataatt ccaagaaaac
     4501 tccatttgaa aagaacgcca aaatacgtga tttagctctt ttttcgttat tttgctgttt
     4561 ttgatctcag aattcttcat cttcttctac ctcccctgca gttgtttgaa gtggtagttg
     4621 acggacattc ataatattga ctaccacaac tgctaaaatc gcaccaaaaa tagctactcc
     4681 ggtcacagga atctctaagt aagaagctat agcaaaacct aagaaaaaat atggtgcaac
     4741 ttgtttgttg atcagcaaac gagcaagcat cgcaaaacca agagctggaa tcaaccctgt
     4801 agctactgac aacccctttt gaacgaagtc aggaatcata tttaatagat ttccgatcgt
     4861 attgcttccc accatataag aaaccattac cacaacacca agcatcaagg aaagaccgat
     4921 accggcaagc aagtgcatat gctcgactcc tttataattt ccttcttctg cataattgtc
     4981 tgctttatgg ttcataattg gaatcaaaag ccctaagtaa atatttttca atatcaatgt
     5041 caacgtagca atcggcaaac ctagtaaaag cgcggtttct gtaccggctc cagctgtaat
     5101 agcaaaagcc gtccctaaaa tcccaccggt cacaacatca ggtggaatcg aagcacctac
     5161 tgagaacgat ccgataaatg ctaactctaa agttgcgccc ataatgatcc cagtttttac
     5221 atcacctaac acgattcctg taaacagccc cgtcacaatc ggacgagaaa gcaacgatgt
     5281 ccctagtgca tattctgatt gtgcgacaaa tgcaacaatt cctaataaga ctgcttgtac
     5341 aatcattgag gttcttcctt tcttacaaaa cttctcttac atccactttt ttatctgctg
     5401 gtacttgacg gatttctact tctatttgtt tgtcaattaa tcgtttcaag cgttcctcct
     5461 cttctggtaa aatatttacc gcttttccga tgctgcgagt accaggtttt gccttgatcc
     5521 ctcctaagtt gatggttttg atttctggat acgcttctgc cagcttatat gcatcctcga
     5581 tcgattccac tacgatgaat aacttatatt tatctgtcac cccgcttttc aacgcggcaa
     5641 tcgagtcatc aattgatttg atcactagtt tgactccttg tggcttggct aatttgatcg
     5701 ttgtctttcg tatctcattc gtaggtacat catcattagc aatcaaaata cagtctgcgc
     5761 ctattgtttg tgtccatgaa aaagctactt gtccgtgtaa taaccgatgg tcgactcttg
     5821 tcaatacaat catttttctt cctcctaaaa ttcgtcttct tctattttct gattgagccg
     5881 ctcgttacaa aactggatca ttttttttga atcagttagc acctgctgga tcaaaacagt
     5941 tgtctctact tcagcgtcca accgagatac tagctcaatc aatagtggca aattcaatcc
     6001 ggcaacgaga tagacatgat cccgattgat atattgtaag aattcgttgt tgacacttcc
     6061 gccaaaaaga tccgtcagaa caatgatttc tttatctttg tacctcataa aaagctgcct
     6121 tactgtttcc gctaaatcaa aattctcatc agtatagcaa tcgaatacag taatagaatg
     6181 atttttccca tagatcagct ctaatgaatc taatatccct gaagcaaatt ttccatggga
     6241 agctaagata atttctctat ccactttttc atctcctctt ttcacttaca catactaaaa
     6301 aagcaagttg catgccaaaa ttagtggcaa caacttgctt atgttcaaaa ttcttcttct
     6361 atcgagagaa actctgtatc ttcactcaaa atatcataga tataggcaat ctcagaattg
     6421 gggatcatga cactataatg ctcttcaatg acactaaacg cagatttaat agcagataac
     6481 ccttcttttt gacactgctt ccatttctct aatccttgat aggtttcgat tggcatttgt
     6541 cggatcaatc gttcaatgag acagctcaca tggacataca acgctaattt tttgggattg
     6601 ctgatgcttc gattcaactg cacctctaat tctctcataa aatgatcgat ttctcccatc
     6661 accttctctg tatctagaat agtcacggaa tccagtactt tttccaatga aaaattacga
     6721 atgatgcgct gattgaatat ttccttctct gaagcattca tagtagtgtc caaccattca
     6781 gctagtacag acatcttttc ccccgagatg aggtcttcta atgaaagata gtcgatattg
     6841 gtgatatcag gatctgtggt gccaatcatt cctacgactt cgtatatgga aaataatggg
     6901 tcactgtttt tcttatcttt taattgctga tattcatatg ggatgatttt caactgacaa
     6961 gccgtaggta agcttttttc tagcagttca cttaagtgga ccgccgtacc gattccagtg
     7021 aagcaagtag taatgagtgc tttgggtttg ttttcctctg gatagagcag tcgccattct
     7081 ggctggatct gtcgaacagt ctcttctgct atctcttcta atgatacatt tttttgcagg
     7141 cattccccta ctgtgattgc taaaggtgtc gtcacattat tcaaaatcaa aagcggtacg
     7201 gataactgtt ttttaaaaaa ctgatggatt tctttcagag atcccatgtc tactaatatg
     7261 atcaatccat ttgaaacatc gctgttttcg caataatcaa ggatttctga agcaatctgc
     7321 tgtggtgtga catctagtgg catatcaaat gattcaaata gatcctcttg taataaacga
     7381 tttactacat ttgccatact gctagcagtt gcataaccgt gagcagcaat catcgctttt
     7441 ggcagtcgtt tttcttttga ccatttcgct ttttttagat agatcgtcag taaaatccga
     7501 tccatacttg tgtcttcaat atctaatttg atcttactta attccagaat tcgttggaca
     7561 tagtgataac tagatggata agactgagaa acttgttgtt ctaattcttt gattagttgg
     7621 ataatatcat gttcttccgg taaccatttg atagtgcttc gctgatagag atagtaactc
     7681 aaggcataga cactgtttcc atcgaatttg atttgatagg cagtttccat ttgtcgaaag
     7741 gtctctcgga tagattgtgt ccaataaacc agcagctcgt gtttttcttg tctactggtc
     7801 tcaaacagca aaaaatcaaa taattgaatc acttcttgct ttaattttcc ttcgcaagaa
     7861 tatagcagac agttattttt cttgaaatca attaggattt gctcaaaact tttaacgatt
     7921 cgctgctgtt cgggctgttg cttctccatt aattgctcta aggtctggtc ttgcgtaata
     7981 tagattcctt gctgtgctgt atttttagta ataggctgcg caagtagttc tttttgcaaa
     8041 tgatggatcg tgacatggat ttcaggctgt tcacgctgtt cagcatatgc ttttgctgct
     8101 gaaactttga cgatattttt cagctctcct atgtttccgg ataatttctg acttgttaaa
     8161 agtgacaaag ctggtccact gactgacaaa gggctcccca tttttctttg ttcttcgaga
     8221 aaaaaagagt aaatcagttc tagccgttca ttacgactac gctgattaag tgcaggtagt
     8281 tcgattcgga taggtattct tcgaatgaaa gttgttaaga aatgactagt aatctcttca
     8341 gtcgtagcaa aaaagagacg tgttttgatc cggcgtgaca aattaggttc tcccatacga
     8401 taaatcacac cttgatcaag ataggtgaac agtttttcct gaccttctgg tgttaagcga
     8461 tgtacctcgt ctaaaaacaa gatcccacca tctgctgctt caaaagcccc ttttttattt
     8521 tcttctgcac cagtgtacgc tcccttaaca tgtccaaaca aattgctagt caaaagttca
     8581 ggattatctg catattgtgc acaattaaaa gtgatgaatg gtgcagaatc ttctagcaga
     8641 tcatgaagca gacaatactg atagaccaaa ttaactaaat aacttttacc tgtgccactt
     8701 tcgccagtaa tcaaaagtgg taatccgcca tctggatagt aaagagctgt tttgatttgt
     8761 tcgatacttt ctttcaaact ggcgtcgtat ccgatcataa aagagaataa gtcttttggt
     8821 tgcgcgaaaa aaggctgttc ctctttgaga tcatcgatag aaaaataaaa attcgtgcgt
     8881 aacttaaaaa attgtttttc aaaagcttct ttgtggaaat aaatcactgg tcgagaattg
     8941 attttgacta gtagttgctc atcatttaac tggttcaaat aatgactgac tgtgtttctt
     9001 tttaccccaa aatgatcagc taacctttgt gctgtaaaga cctcgttcat atgttctaaa
     9061 tcgaaaaagg ctgtctggtt ctctaagtaa cataaaattt ccttcttcaa caacttattc
     9121 acccctcctc catcatagaa aacggattca ttttgtcaat aaaatcttta ataaagaatt
     9181 gaaaaatata tcttattata gacagcagcc tacttatccc ttataatttt tattctaaaa
     9241 aacgtacagt tccttccagt ttttatactg gaatttcaac atatgaaaga ataaggaggc
     9301 tatgactatt caatgaaatc ggataaatat aaaaacagga aacatacttc ctggaaaact
     9361 tttcgagaaa tcatcggatc ttatctcgct gtagttgtat ttttgccttt gctttatatg
     9421 gctatgacag aacaaactcg ttttattggc gtagatacgc aatatatagc cgaaacagga
     9481 tttttattca tctttatctt gatttttgcg ttctataaat ccttccatac aaaataagat
     9541 tcacagattt caactttcct gtctgttttt agctattaca aatccttata tgtggtataa
     9601 caacaattta ttggcataat gaggaaaaac gagttatgat gctttcagag agtaaagagg
     9661 tactgttgtc accatccttt ttattgatta cgtacaccac tcttagtttt ttcccattga
     9721 ttacgaaaac aaggtagaaa cattgatgtt cctaccttgt tttctttttt tattttcaga
     9781 tcaaaatctc aagtagcttt tgacttgatg tcgggaggtt gtcttcgttc catcaaacct
     9841 gatagaatct gcgccaaacc atagttttct ccactattat ttattcctct aagatagtac
     9901 ccacaataaa cgaataaata tttttctcat tttcatactc cttctttgaa aatcaattag
     9961 atttctctca acccactttt ttacaaaaat tacgtagcaa aaaagattgc aagaacaaat
    10021 taaaaaagaa gtaaatagag tttcatctat aaacatttac ttgatatatt ttttatgcag
    10081 aattca
//
LOCUS       DAJSKS010000101        10055 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJSKS010000101 DAJSKS010000000
VERSION     DAJSKS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.103"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(19..171)
                     /locus_tag="ON396_002082"
     CDS             complement(19..171)
                     /locus_tag="ON396_002082"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCR3114152.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(362..1240)
                     /locus_tag="ON396_002083"
     CDS             complement(362..1240)
                     /locus_tag="ON396_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3114153.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1279..2397)
                     /locus_tag="ON396_002084"
     CDS             complement(1279..2397)
                     /locus_tag="ON396_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCR3114154.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2394..4493)
                     /locus_tag="ON396_002085"
     CDS             complement(2394..4493)
                     /locus_tag="ON396_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HCR3114155.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4544..5365)
                     /locus_tag="ON396_002086"
     CDS             complement(4544..5365)
                     /locus_tag="ON396_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3114156.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5362..6171)
                     /locus_tag="ON396_002087"
     CDS             complement(5362..6171)
                     /locus_tag="ON396_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3114157.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6192..6686)
                     /locus_tag="ON396_002088"
     CDS             complement(6192..6686)
                     /locus_tag="ON396_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3114158.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6698..7186)
                     /locus_tag="ON396_002089"
     CDS             complement(6698..7186)
                     /locus_tag="ON396_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3114159.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7251..9947)
                     /locus_tag="ON396_002090"
     CDS             complement(7251..9947)
                     /locus_tag="ON396_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3114160.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         3055 a         2264 c         1657 g         3079 t
ORIGIN      
        1 tacggctgaa aaaatctatc accaaaaaaa ttcaaacatt aagccaagga ttatgaagtc
       61 aacatttcga aaaatgaaca tacattggta agcaactata actattacca cagacataaa
      121 gtaacacaat atattactat ttaagtgtac gccaccagtg aatatcctca aggtatacag
      181 cgcacaacaa aaagaaaaaa ggaaacgcag aagcatttcc tttcagtgat ttaatgaaaa
      241 caacacgatt tgacttagag cctttttatt tgaattcatt ttcgctttat ccaacttcat
      301 ttgatcattt gattactcgg ttaaatactt ttgacatgag tatcaatgaa acattcatta
      361 attatggttg cttatttctt accgcttttc tggccaatgc taatccgccg attattccgg
      421 cattatcacc taattcgggc gtaataatgt agctatcaat tggtggtatt tcaacatatc
      481 catttataag ctcaacaaat ttatcctgaa cttttttctt catgtgttct tgtttcatta
      541 caccaccgcc aaaaataatt ctatctggcg aaaacatcag tgttgtattg tatgcacatt
      601 gggcaaggta aaaggcttca atctgccaaa acgaatcgtc agccaataaa ttctggcctt
      661 ttacacccaa ccgtttttca atcgctggtc ctgctgccat cccctctaaa caatcttggt
      721 ggaagggaca gtttcctgaa aaattgtcct ttggatgtcg cttgatcaac atatgcccca
      781 tttccggatg actgaaccct tccacgaaag tcccattgaa caaggcacca cctccgatac
      841 ctgttcccac agtgaagtat gcaacacctg ctaacccttt gccagaacca gcaacatatt
      901 caccatagca agccgcattg acatctgttg tccattcaat cggaatattg aaatattgct
      961 ttagcgttcc tacaaaattg aaattttgcc aagcaagttt tggtgttgat gtgatatgac
     1021 cgaaactcgt tgatttgcat tgaatatcaa ttggaccaaa gcagccgacc ccgatgcctg
     1081 ccaattgttc cttaaaaggt ttaaaaaaat caatgacctg cttcattgtt tcaactggag
     1141 ttgttgtggg aaaactgact ctttccaaaa tcgttaattc ctcagtaccg actgcacaga
     1201 caaatttagt ccctccagct tcaattgccc catatatcat cataggttcc tcccttcgaa
     1261 tatgtgaaaa acacttcatt aacgcggcaa gcgttctcga tcagtgaaat gacgtttagt
     1321 atttcctgaa gcttctgttg aataccagta agcgacacta gcaacatcgt cttgccgctc
     1381 aaataagcga acatcatcgt tgccgatttg ttgtaaagtg accgataaat cttcggtaaa
     1441 acagatgggg tcttgaagat gccaacgata aagaccatgt cgtggcattg cgtcgtcacc
     1501 aaaggcatga acggcattca attttcccgt ttcaaaacga tctcttgtgg catctctatt
     1561 tgattggtaa ggatacccta aaaaggctgt tgaatagttt ttcgcatgtg gacgtccgaa
     1621 ctcatcgtac tcatgaaagg cccacgcccc gccaaaataa tcttctgaac ctgttgaggt
     1681 tacggtcgga tactcactat ccccatcaat gtagaattta aactcgcctt cgccccacca
     1741 ataacgttct aggcaggtaa gcgcaaggta agtacccacg taatagcctt gaccctttaa
     1801 tcgatcaatt aacttataat cctcctgtaa tttggtaatg cgttcccgat gccaatttgc
     1861 gtgaaaatat aggctatttt cttccaattc gtccaccaaa gcgtaattta tcgtataaaa
     1921 aaatgaggac atatccttcg gatgtaaatt ctcgatcgta attttagcgg atgtttggaa
     1981 aggcatttcg aaataggaat taaagccccc tgtaggatta accactatcg gcaacgagtt
     2041 cacctcgcaa cgctctccaa acccattaca aaagaaatcc cctaaaggac tttcaacagc
     2101 agggctatcc gacccatccc aataaattct taagattaaa tcacgtaaaa caaaactgcc
     2161 aaattctgtt cgatcacgaa tcgtcatcca catatgacga attattcccg ttccagtaat
     2221 atccgcaatc acagtttctt caccagaagt cagcggaatt gcgccacgac ctttgcggct
     2281 tggtcctagc tcgcttggca acatcgcagc tttacctttc tcgcctgttg gattttcggg
     2341 agtgattgct cgactctgga tttttttaaa ttgggtaata ttttttagta tactcatctt
     2401 tttctccttt gaatgtgaat cgtttttccc ttttctacgt gaagaatttt tttcgtatca
     2461 cttattttca atggcttttc tgacaaattt gaaatactca actcggattg cgtacattca
     2521 atcgaaatga aggcttgacc gaacttcaaa ttcttccaag aaaacgttaa attatcaaca
     2581 ggatcaattt gcaatactcc ttttttgaat ttcaatccga aatattgact gatcattagg
     2641 ctgacaaaaa gacccgatgc ccagccgcat ttgccgcagc caaagtctcg cacgacttct
     2701 ccttgttgag cctctaagcg atacggccac caccaccacg aaccgtctaa atctgccaaa
     2761 caaattagtt cttctaatcg ggttacgaaa gcttgagggt tgtgcagctc gctcattaaa
     2821 acagtgataa agccaggaaa agtggcgccg gattctaatc cccaagtgat gcctttgatt
     2881 tcctcgctat aggttggatt gaaagaacta gcagaaaagg ccatggtgtt ctgatataaa
     2941 tgatctttat tatctaaaaa ttgataaaaa ggcatcaacg tagtgtctga ttcttctcca
     3001 tcatgcatca ataaattaat ttttccatcc acaatcacat cacttaaaaa gattaaccct
     3061 tgctcaagaa tcggtttttg atagtgcttc actgaatagg ttgttttctc ctcatcaccg
     3121 attccctcca aaaattgctc accaaaagga ccatcgatag tcatttgttt acaaattgat
     3181 tcctttagtt tacaggcaat gtttttgtat cgcttgctaa gactcagttg ttctagggct
     3241 tcaaaaattt ctgcaagtcc aatacaggcc ttccaaagac aaagattgct gccggtatga
     3301 tactttccaa gtgcaaaggc gtctgataac caaacagatg gaaaaagcat tggttcccct
     3361 gaccgacgtt gagctaatat gtcctcaata attcttgaag cattcagtag aacctgcgga
     3421 caccgctcaa agaattttgt atccccagta tgttcaaaat aaagactagc cagtaacgca
     3481 ctggataacg aattactcag agaatgattg atacctcctg taaattttga accaggaaac
     3541 ttaacgccat attgatcaaa ccatagaacc gttttttgac ataactccgg ttcaaacatg
     3601 gtgaatggca atgatgcgta atacatatcc ttattccaaa ttcgattagt agcaggatag
     3661 ctgccccaat tgctgccgac gacctgtccc tgtcgattca taccgaaact agaaaagctt
     3721 tgataaagtg cacgacgata gagatggaca actctatcgt ttggcattga cagctgccca
     3781 aatagctgag caaaatagtt caatgtttcc ttcagccatt tttcgatatc agattcctga
     3841 aaagtcatta cttcttcaaa agcatttggg tccacaaaag caatggcgaa gcttgccact
     3901 tcctgttgtg cgatcgtgtc cttatttcct ttttgagaaa tcaacacatt ttgctgatcc
     3961 gaatacttct cttgatacga aggcacatct ttcactgaaa cttgaaggct ttcgtgtgtt
     4021 tcattcacaa tgtaaacctg ttgaacaagc atacttaaac gcttattagc cagtatggga
     4081 gcaaaggata ctacaatgac cttaaacgtc gaataatgat gaatatatct aggcaaacag
     4141 tcttgaatca catcgacttc aatacgatga tccgattctg acaaatggta gacaatcccg
     4201 tttacttcaa cctttaagga taacgaattg gtttgagaga gctgtttatt gacccaaaca
     4261 cctggttttt cctcagttag taaattttct cgataaaagg tcaacgtttg aatcgtcccg
     4321 caaacatcaa ggtttgctaa tgtcttttga ttacttacat cgaaggtcaa tccctttaca
     4381 tcaggaatat tccgatagta gctgtattcg tttgaagtag tcacatttcc gtcgtttaac
     4441 tcagcattaa aaaatgggtg tttgggtgaa taaattactt cattaatagt catcttttcc
     4501 ctccttgcta aaaaaactgc agcctagtag ctgcagcttc ctactatgcg attccaagta
     4561 atgacaataa aattcccaag acgaccattc caaaaataat tttgatgacg ccgacttttt
     4621 tatctagtaa tttgaaacag ctcaatgtta ccaataacgg tacaattcct ttaaaaatac
     4681 tatcgaggat ttcttggaca ttcattagtt cttcgccctt cattgaaaag ctcaaaacgg
     4741 tattgaattt gaccatactg atcgtcattg cacctaccat gatcagaccc aagatactag
     4801 cagcttttgt cagaatttca attaacccgt tcgcatacag cttttcaata tatttagagc
     4861 ctaatgaata gcctaaaacg ccgccatagt atcgcatcaa gtaattaggt atattgaaca
     4921 taactaaaaa gataattggt gccaaaatat ttccggcatt ggctaagttg atcgctagtc
     4981 ccgcggtaac gacacgccaa actccccaga acaatgaatc gccaattcca gccaatggtc
     5041 ccatcaaact cgttttgatc gcgttgatac tttccacgtc aaaaccagct ttttcagaat
     5101 tttccttctc catcgatgcc gcaatcccca tgacaaagga cgcgcaagga atggtcgtcg
     5161 aaaaatactg tgtatgacgt tccagcgccg cagctcgatc ttcttcactt ttgtagaaat
     5221 gattaatgaa gggcatcata gaataggcga aaccaccgcc gcccatcgtc accggattca
     5281 ccgaagcata catagtcatc gagcgccaaa acatcttccg aaccaatttt ttctcatctt
     5341 tagaaattcc atcagctaat ttcattgaaa gaaatcctcc tcttcttggt caagcgttgc
     5401 atttacagca gcatcgcttt gaactgcatg aacagatata ttttttagtt gtagatctct
     5461 ttgggctata acaattgcaa taatcgttcc aactgcggca attgcaatca atggtaactc
     5521 gagatacgcc ataacaacgg ccccaaagaa aaaccacaca gcaatatttt tactccaaag
     5581 catccgtaac agcatcgcca tcccaactgc cggcagcaga tttccagcga ctgttaatcc
     5641 gttcatgatg acctctggaa tattatccaa gacgcttttg acaacatctg ctccaaataa
     5701 aacggcaaag aatggaataa gagcataaag cgcatagtgg acaaaccaca taaagaagtg
     5761 cagattgatg attccttttt gatcaccaga ttttgcaaat ttgaaaaatt tagcagccat
     5821 cggtccacat acaaaagaaa aaatcatatt cattaactgt aatcctaata ctgctagtgg
     5881 aaagacaatg ggaataatcg catccacgtt tcctcccatt ttgattgcga acgctgtagc
     5941 cagcccagca gccaaagaag gctccgccgt actagagatt ccgatattga cgacacccat
     6001 aaagatggct tctagtgacg ccccgacaat caaccccgtc tgcaaatccc ccatcaacgc
     6061 tcctgctaac ggagcaacaa caatcggacg actcagcatg gacatcccga acatttcatg
     6121 tccagcaact ccaataaaaa cggccaaagc gactacaaat gcatcataca taatttttct
     6181 ccttcctttt tctaaaaatc actcaaaata gagatagctg aacgtttttc catcgccggt
     6241 gtaggctgaa aaacggtatc tggataaagt tcaactaatt cttttaagtc ttgaatttct
     6301 gacgtcgtta acatcacttc ctttgataag gttttcttat cttccgatgg cggtgtcatt
     6361 ttcccagcat tccccacgtt cattaattcg atttccgttg ggaaagcttt cgcaactttt
     6421 accgcatctt tgacattagg gactaaaacc atcaagcgca tgtccttcgc ccgcggatct
     6481 tttaacaaat gaatcgcgtc atcaactgtt ttaataatga ctttgacatt cgatggtgca
     6541 gccatcttca atgtcatctt ttgaatatcg ttggtagccg cctcatcgct tgctactact
     6601 aacccggtta aatttagact ctttgtccaa gtcatcgcta cttgtccatg aattaatcgt
     6661 tcatcaattc gcactgcttt aatcattttt cttcctccta aaaaaaatct tcctcttcat
     6721 ttactacatg tacctcatca agaatggtca tctttaattc ctgttggcat ttatttacca
     6781 gttcctgaat atcctccttt gttaattcct tggttgccaa tactatttcg agcagaattg
     6841 gcagattaaa gccagacaaa atagttacag caatctcttt ctccaaaaag gttttggtaa
     6901 tcttctgatt gacacttccg ccaaataaat cggtaaaaac aaaaatctga tcctgctcgt
     6961 tcacctggtt tacaaataca ttcaactcct gttcaaaatc acactcatcc acataagcat
     7021 taatcaccgt aatctcttga accttccccg tcagtaattg aatactgcta agaaagcctg
     7081 aagctaagtg accgtgagtc gctattagaa ttttcttcat atggttaatt cctttctttc
     7141 tgttcacttt tatataagca attcctgtgc caacttttaa aaacattggt ttaatcacgt
     7201 taattcacat taaaaaaagt gtctaaccta aaaaaaacag attagacact ttatttttga
     7261 atatacggtc ctaataattc atagagcagc gaaagctcat aagcattgac tttaaaatta
     7321 tatttcatct ccattgattg aaaaatactt ctagaaacat aataaaattc atcttcatct
     7381 ggtttcaaat cataattcgg ttcttcagat ttttctgtcc gagccaagaa taaacgttcc
     7441 atcatcaatg ccgtatgcat atagagattt agttttacac ggccatttaa agtgaaatga
     7501 tagtaatttt catatttaga aatgactgtt tccacttcac tcatcacgat cgttgggttc
     7561 agaaagttca aacggtccgc aattccttcc aacgaaaaga attttactag ctcctgaatc
     7621 aagcggttaa attctgccgg cttcatttca ggactcaaaa tatgctccaa attggctgct
     7681 ccatcaccgt ctaagatgtc ataaacatta atattaggaa ttgaaaagga acgtggaaga
     7741 tccgaagtcg tgatgaccaa ttttgtcttg tcaaaataag tttcatcatt ttggtcgatt
     7801 aattcacgta gttctctgta gtccttcgtg aaaacttcga tggattcatc cgataaatga
     7861 tgacgaaaaa catctttcaa tttgtccgaa atccccaaac ccgacataca ggaaatgata
     7921 atgttgctac tttgagaaaa gccttcaaag tattgaacat tgattgaata cttgtctgct
     7981 gcatcctccg caatctgttt aaatggcgca ttcatcgtca tcttcaagcc aatatcaagt
     8041 gcaatggaag tcgtcaaatt attcagaaga agtaactccc cttttaaatc gttcttgatc
     8101 tgtgtgtaaa tttgatttaa agagcccata tctacgatca gcactagact atctgtattc
     8161 acttgccgtt tcacgaaatt ccttgtcgat tcgacaatct ctgacacatt actctctact
     8221 ggcatatcca acgcttcaaa aacataattg ccgcataatt gattcactac tgcttgaatg
     8281 ctgctagccg tactatctcc gtgagcaagc aacaggccct tcagctcgat ggattcatct
     8341 acataatcag ataaagtcac gacaataagc agtcttagaa agttctcgtc cttttctgag
     8401 agcggtggta aatgctccaa gaactgatta cataagtaag tcgtccgagg tagatttgca
     8461 gctaatgtac gatttatccc ggttaagtct gtcttatagc ttggggaaat gacataggat
     8521 ctgctaataa tttctgcaac aggtccactc gtcgtcaatc cgtacgtttt tcctaaaacg
     8581 gatacccaga cctttctaaa catctgaatc atcaattttc tactcattga taaaaaagaa
     8641 tccttatcaa tgtgatgcac caaatgctgt acttcattct ttatcgaaga aaaattttca
     8701 ttgaaattac cttcatttat cagctgagta attttttcca aaatcgaatc ataatcaaac
     8761 ggtttgattc gatccgtttt tatcttttga tttttcttta tcaataaact agcaatcggc
     8821 aagctttcta cttcggattc cagcatattt aactgaatca agatctcatt tttcccaatc
     8881 gttaatggtt cttcttgtgt tcgactatac acatccgcac aactgatttt aatgatattc
     8941 ttcagcttac caatattccc actaaaggga tgagataaca ttaaagacaa ggcttctctt
     9001 gaaacaataa tatctttttg taaaacatcc gcttcatttt gaaaaaataa ttgaatcagc
     9061 tctaatcgtt ctcgaatggg gcgatcttca aaagttggca acataacact aatttgaata
     9121 cgccgatcaa aggtgtctaa taaataattt tcaccttttt ccgtcgtggc gaaaataaaa
     9181 cgaaccttag cagaatgcca atttacattt tcaccgactg gacgatagtt tccagtatca
     9241 ataaatagaa agagcttttc ttgattttct ttcgataggc gatggacctc atcgagaaaa
     9301 agatagccgt tgttggccgt tgccaataaa ccatcggtat ccttttcagc ccccgtaaag
     9361 gcccccttct tgtatccaaa cagtgttgcc gaaagcagtt cgggattgtc cgcataatcg
     9421 gcacagttta agatagcaaa tggtgcatct ttttcaataa tttgctcatg gatagcgtat
     9481 tcaaaaattt ttgtggctaa aaaacttttt ccaacacccg tggctccatt tattaataca
     9541 ctgagtccgt taggcggata tttgacagcg gctatacatt tttgaatgac ctctctcaga
     9601 cttccgtcat gaccaactac agaatgaaag gcttcgttta tttgctccgt attatctaca
     9661 accgcatatt catctaaagg cttccacaaa acaggctttc ccggtaattt ctcgaccttt
     9721 ttatcttcca ctaatctcgt caaataatgg ctggcattct gacgagagat acctagattc
     9781 ttcgaaattt tctcggcagt gacagaagac atattccctg ttagtcgtat cagctcatca
     9841 aaaactcgat ctttagcgct ctttttcatt ttttcctcca gtttaatgtg tctaaaagca
     9901 taataaacta tcggctgcaa tatgtctatt ctatatttgt attccaattt ttactttgaa
     9961 acacaaatag tcgaatattt gcctcagata tctgacaaaa taatttatct accaatttag
    10021 tttttatcta atatgatttt tatgtttttt catag
//
LOCUS       DAJSKS010000102        10022 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJSKS010000102 DAJSKS010000000
VERSION     DAJSKS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.104"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..722)
                     /locus_tag="ON396_002091"
     CDS             complement(<1..722)
                     /locus_tag="ON396_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR3114161.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RCWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRKLQNIGAYQYFEKQNIIISQSTHGGA
                     AENSYEGNVKNLQSYQNVQLSKRAIEILKEEIELNHQHIRFNPDYKDNGWIFTSKSRH
                     KPGMATVFLTNFIRCRTSFRML"
     gene            complement(727..1494)
                     /locus_tag="ON396_002092"
     CDS             complement(727..1494)
                     /locus_tag="ON396_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3114162.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1983..2408
                     /gene="lacA"
                     /locus_tag="ON396_002093"
     CDS             1983..2408
                     /gene="lacA"
                     /locus_tag="ON396_002093"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HCR3114163.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2425..2940
                     /gene="lacB"
                     /locus_tag="ON396_002094"
     CDS             2425..2940
                     /gene="lacB"
                     /locus_tag="ON396_002094"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HCR3114164.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2951..3883
                     /gene="lacC"
                     /locus_tag="ON396_002095"
     CDS             2951..3883
                     /gene="lacC"
                     /locus_tag="ON396_002095"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HCR3114165.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3886..4866
                     /gene="lacD"
                     /locus_tag="ON396_002096"
     CDS             3886..4866
                     /gene="lacD"
                     /locus_tag="ON396_002096"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCR3114166.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            4894..5211
                     /locus_tag="ON396_002097"
     CDS             4894..5211
                     /locus_tag="ON396_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR3114167.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            5211..6917
                     /locus_tag="ON396_002098"
     CDS             5211..6917
                     /locus_tag="ON396_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3114168.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            7059..8465
                     /gene="lacG"
                     /locus_tag="ON396_002099"
     CDS             7059..8465
                     /gene="lacG"
                     /locus_tag="ON396_002099"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCR3114169.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRLKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            8588..8761
                     /locus_tag="ON396_002100"
     CDS             8588..8761
                     /locus_tag="ON396_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HCR3114170.1"
                     /translation="MKLPSLLKCGKWYVLTGKEWICHSDLELSAFEWEFLQSLDVEIR
                     ETIHFEVNYLPFQ"
     gene            8782..9681
                     /locus_tag="ON396_002101"
     CDS             8782..9681
                     /locus_tag="ON396_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCR3114171.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDEDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSSLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         3164 a         1690 c         2006 g         3162 t
ORIGIN      
        1 aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac tgttgccatt
       61 ccaggtttat gacgactttt ggaagtaaaa atccatccat tatctttata atcaggattg
      121 aatcttatat gttggtgatt taactcaatt tcctctttaa gaatttctat agccctttta
      181 gataattgga cattttggta actttgtaag ttttttacat taccttcata gctattttct
      241 gcagctcctc cgtgagtgga ctgactaatg atgatatttt gcttttcaaa atattgatag
      301 gcaccaatat tttggagttt tcgactctgt aagtcaacag ggccattagt tagaatacct
      361 attgggatgc ggtttttatg tagagccagc agtagttgat gtatctcagg aatcatcgta
      421 atctgatcga gttcatattc atagtcagct tggaagtcta gagcttctgt cctagaaata
      481 ctatcgatgc caaattcttc catagtcttc tgacagcgaa aaattctaag ctgctcaata
      541 ctgatgagtt ttttcgtgta tttaggaaaa gcaacatcag accaacatct gaaacgtttg
      601 taaatgttat ctatttgttg gatatcaatc gttggaaaac atttctcaac gctacgttta
      661 aaggggagtt gatggtcata aagagtgtca tcaaggtcaa aaacaatatt tttataagtc
      721 ataattctag tccgctttta aaatagtagt gtactggcta aattcctcta agtgttgagg
      781 gctaatctgg ttatctgtaa tgatggtatc gagttgatct agattataga agttaaagaa
      841 atcgtatcga tcgaatttag tactgtctac taataagaat ttttctacag cattgtttag
      901 ggcaagttgt tgaatcacac cttccttgtc actatatgta gcaatagaat tatgggtaac
      961 agcatttgca ctaacaaaag ctttggcaaa tctaattgct tttaaatttg tcgaagccat
     1021 tgaacctaca aaagctccag ttatttctct atattcaccg ccaagaagca ataaatcaat
     1081 ggtttcgcta tcatttagaa tcaagaacac agggagacta tttgtaatga cacgaatttt
     1141 ataaccttta cgacctttca attctaatgc cagttgttca agtgtagttc cgggtccaat
     1201 aaaaatagta tcgccatctt tgattaaaga gcaggctttt ttagcaatgt caattttttc
     1261 ttttgtattt agacttttct tctcgatatg tgtcttttca agtggctttt tagaggaaag
     1321 taattgtgca ccaccatgag tacgtgttaa aagtccatca gcttctagag catcaaggtc
     1381 tctacgggct gtcatatcgg aaatatctag ttcatctatt atttctttta tggttatggt
     1441 cccatcaatc tttaacatat ctaaaatttt ttctaatcgt cgttttttgt tcatatgaag
     1501 acccttcttt cataaagtaa tttttttcca aagataattc tcttttaatt gtatcataaa
     1561 agacaatatt ttcaaggtaa aacaaacaat ttcaaacaaa aacaaatgtt agatgatgaa
     1621 ataagaacag tggattgacg tatattagct taggtcagat tttgtataag acgaaaataa
     1681 agtaggacct cttaatcagt aagttataga aagtaaaaga cttttgtaat acttgaatag
     1741 atatttcacg tccattttgt gatggattaa atgaacaaaa atgaacaata atttaacggt
     1801 gttatctatt ttttaagaaa acaaataaaa aaaacaaaaa ataaacaaaa aagtgttgct
     1861 ttttgtttga atgattgata ttatataaac aaagaaatga tgaaaacgtt ttttcgaaca
     1921 ttttgcaaaa tgttttctac ttctacgtag catttctttc taaaatttag gaggtggtca
     1981 aaatggctat tgttgttggt gcagatctca aaggtacgag attaaaagat gttgtgaaaa
     2041 attttcttgt agaagaaggt tttgaggtaa ttgatgtgac taaggacgga caagattttg
     2101 ttgatgttac cttggcagtt gcctctgaag taaataaaga tgagcaaaac ttaggtattg
     2161 taattgatgc ttatggagct ggtccattca tggtagcaac taaaattaaa ggtatggttg
     2221 cagcagaagt ttcagatgaa cgctcagctt atatgacacg tggacataac aattcacgaa
     2281 tgattacggt tggcgctgaa atcgtcggtg atgagcttgc taaaaacatc gctaaaggtt
     2341 tcgtcaatgg taaatatgat ggcggacgtc atcaaatccg agtagatatg cttaacaaaa
     2401 tgtgttaaga aaaggagtta aggaatgaga atcgcaattg gatgtgacca cattgtcaca
     2461 gatgaaaaaa tggcagtatc agaattcttg aaatcaaaag gacatgaggt ccttgatttc
     2521 gggacatatg accacgcacg tactcactac cctatctatg gtaaaaaagt cggtgaagca
     2581 gtagtaagtg gtcaagcaga cttgggagta tgtatctgtg gtacaggtgt cggcattaac
     2641 aatgcagtca ataaagttcc aggtgttcgc tcagcgttgg ttcgtgatat gacatcagct
     2701 atatatgcaa aagaagagtt aaatgcgaat gttatcggtt tcggtggaaa gattactggt
     2761 ggtcttctta tgaatgacat cattgaagct ttcattgaag ctgagtacaa accaacagaa
     2821 gaaaataaaa aattgattgc aaaaatcgaa cacgttgaaa cacataatgc acatcaagca
     2881 gatgaagatt tctttacaga attccttgaa agatgggacc gtggtgacta ccacgattaa
     2941 ggtataacaa atgattctga cagtcacact aaatccttca gtagacatct cttatcctct
     3001 agaaacattg aaaattgata cagtgaatag ggtgaaagat gttagtaaga cagctggtgg
     3061 aaaaggctta aatgttacta gagtcttgta tgaatctggt gataaagtta ctgctacagg
     3121 tttcttaggt ggtaaaattg gtgaatttat tgaaagcgaa ttagaacaat ctcctgttag
     3181 tccagctttt tataaaatct caggaaacac tagaaactgt attgctatct tacacgaggg
     3241 taaccaaaca gaaattttgg aacaaggacc aacaatttct catgaagaag cagaaggctt
     3301 tcttgaccac tatagcaatc ttattaaaca atcagaagtt gtgacaattt caggaagttt
     3361 accttcagga cttcccaacg attattatga aaaacttatc caacttgctt cggatgaagg
     3421 agtagcagtc gttttggatt gttcaggtgc acctcttgaa acagttctaa aatcgtcggc
     3481 taaacctact gccatcaaac ctaataatga ggaactttca cagttgcttg gaaaagaagt
     3541 aacaaaagat attgaagaac tcaaagacgt tcttaaagag tcacttttta gtggtatcga
     3601 atggatagtg gtctcattag gtcgaaacgg tgcctttgca aaacatggtg atgttttcta
     3661 taaggtagat attcctgata tcccagttgt taatccagtc ggatcagggg actcaacggt
     3721 agctggtatt gcatcagctt taaatagtaa aaaaagtgac gctgacttat taaaacatgc
     3781 gatgacattg ggtatgttaa atgctcaaga aacaatgaca gggcatgtga atatgactaa
     3841 ttacgaaaca ctaaactcac aaattggagt aaaagaggta taaaaatggt acttacagaa
     3901 cagaaacgca aatctttgga aaaaatttca gataaaaacg gtgttatctc agctttggca
     3961 tttgaccaac gtggtgcttt gaaacgtttg atggcacagt atcaagatac agaaccaact
     4021 gtagctcaaa tggaagaact aaaggttttg gttgctgatg aacttacaaa atacgcttct
     4081 tcaatgttgc ttgaccctga atatggactt ccagcaacaa aagcattgga taaagaagca
     4141 ggtcttcttc ttgcctatga aaaaacaggt tatgatacat caagtacaaa acgtttgcct
     4201 gactgcttgg atgtttggtc tgcaaaacgt atcaaagagc aaggcgctga tgccgttaag
     4261 ttcttgcttt actatgatgt agacagctca gatgaactta accaacaaaa acaagcttat
     4321 attgaacgtg ttggttctga atgtgtcgct gaagatattc ctttcttctt ggaaatcctt
     4381 gcttatgatg aagaaatctc agatgcaggt tctgttgaat atgcaaaagt gaaacctcgc
     4441 aaagtcattg aagcgatgaa agttttctct gatccacgct ttaacattga tgttcttaaa
     4501 gtagaagtac cagttaacgt taaatatgtc gaaggttttg ctgatggtga agtggtttac
     4561 agtaaagctg aagctgctga cttctttaaa gcacaagaag aagcgactaa ccttccatac
     4621 atctacctaa gtgcaggtgt atctgctaaa ttgttccaag aaacattgca atttgctcac
     4681 gactcaggtg ccaagtttaa tggtgtgctt tgtggacgtg ctacttgggc aggatctgta
     4741 gaaccttaca tcaaagaagg tgaaaaagca gcacgtgaat ggttgcgtac tactggattc
     4801 gaaaatattg atgaacttaa caaagttctt gttaaaacag ctagtccatg gactgataaa
     4861 gtatagtaaa aacataaaac ggaggatatt gttatgaaca gagaagagat gactctctta
     4921 gggtttgaaa ttgttgctta tgctggagat gctcgctcta aacttttaga agcgcttaaa
     4981 gcggctgaaa atggtgattt cgctaaggca gatagtcttg tagtagaagc aggaagctgt
     5041 attgcagagg ctcacaattc tcagacagct atgttggctc gagaagcttc tggggaggaa
     5101 cttccataca gcgttaccat gatgcatggt caggaccact taatgactac tgtcttatta
     5161 aaagatgtga ttcatcacct catcgaactt tataaaagag gagcaaaata atgcataaac
     5221 tcattgaact tattgagaaa gggaaaccgt tctttgagaa aatctctcga aacatctatc
     5281 ttcgtgctat tcgtgatgga tttatcgctg gtatgccagt catccttttc tcatctatct
     5341 ttatccttat tgcctatgtc ccaaatgctt ggggattcca ctggtcaaaa gacattgaga
     5401 ccttcttgat gactccttat agctattcga tgggtatcct agcattcttt gttggtggta
     5461 ctaccgctaa agctttgacg gactcaatga accgtgacct acctgcgacc aatcagatta
     5521 acttcttatc tacgatgcta gcgtctatgg tagggttcct tttgatggca gctgagcctg
     5581 caaaagaagg aggcttcttg acagccttca cgggaacaaa aggtcttttg acagcattta
     5641 ttgcagcctt tgtgacagtt aatgtttaca aggtctgtgt gaaaaataat gttactattc
     5701 gtatgcctga agaggttcca ccaaatatct ctcaagtatt taaagacttg attccattca
     5761 ctgtagcggt tgttctcctt tatggatttg aactcattgt taagggaact cttggtgtaa
     5821 ccgttgcaga atcaatcggt acccttcttg ctcctctctt ctcagctgca gatggttacc
     5881 ttggaattac acttatcttt ggtgcttatg ccttcttctg gtttgttggt atccacggtc
     5941 cttcaattgt cgaaccagca atcgctgcta tcacttatgc caatatcgat gccaacttgc
     6001 atcttatcca agctggacaa catgcagata aagttatcac ttctggtact caaatgttta
     6061 ttgttaccat gggtggaaca ggagctacat tgattgttcc attcttgttc atgtggattt
     6121 gtaaatcaga acgtaaccgt gccatcggac gtgcctcagt tgttccaaca ttctttgggg
     6181 ttaatgagcc aatcttattt ggtgcaccaa tcgtattgaa tccgattttc tttgtaccgt
     6241 tcattttcgc tccgattgtc aacgtttgga tctttaaatt ctttgttgac acattgaaca
     6301 tgaactcatt ctctgccaac cttccatggg ttactcctgg tccgttggga attgtactcg
     6361 gtactaactt ccaagttcta tcatttatct tagccggact cttggtagtt gttgatacta
     6421 tcatttatta cccatttgtt aaggtatacg atgaacaaat tcttgaagaa gaacgttctg
     6481 gtaaaacaaa cgatgctctt aaagaaaaag tagctgcaaa cttcaatact gctaaagcag
     6541 atgccgttct tggaaaagca ggcgttgcta aagaagatgt agcagcaaac aataatatca
     6601 caaaagaaac taatgtcctt gttctttgtg caggtggagg tacaagtggc ttgcttgcca
     6661 atgccttgaa caaagcagca gcagaataca atgttccagt caaagcggca gcgggtagtt
     6721 acggtgctca ccgtgaaatg ttgccagagt ttgacttggt tatcttggct ccgcaggtcg
     6781 cttcaaactt cgatgatatg aaggctgaaa cggataaatt gggcattaaa cttgcaaaaa
     6841 ctgaaggagc tcaatatatc aaattgacac gtgatggaca aggtgctctt gcatttgttc
     6901 aacaacagtt tgattaataa atttgtaaaa gttatttgag acgagattat tctttcagtt
     6961 ccttataata ttgttaacca tatcattgca tttcatttcc ttggtttccg gaactcagaa
     7021 tatcgtctca aatgcttttt ttgaaaggat ttatagttat gactaaaaca cttcctaaag
     7081 attttatttt tggtggtgct acagctgctt atcaagcaga aggtgcgact cacacggatg
     7141 gtaaaggccc agttgcatgg gataagtatc ttgaagacaa ttattggtac acagcagagc
     7201 ctgcaagtga tttttaccat aaatatcctg ttgatttaga attagctgaa aaatatggcg
     7261 ttaatggtat tcgtatttca attgcctggt ctcgtatttt cccaactggc tatggcgaag
     7321 taaatgaaaa aggtgttgaa ttctaccata atctttttgc cgagtgccac aaacgtcatg
     7381 ttgagccatt cgtgactttg caccactttg acacaccaga agttcttcac tcagatggag
     7441 atttcttaaa ccgcgaaaat atagaacact ttgtagatta tgccgctttc tgttttgaag
     7501 agttcccaga agtaaactac tggacaactt tcaatgaaat tggcccaatt ggagatggtc
     7561 aatacttggt tggtaaattc cctccaggca ttcaatatga tcttgccaaa gtcttccaat
     7621 cacaccacaa tatgatggtt tctcatgcac gtgctgtgaa attgtataaa gataaaggtt
     7681 ataaaggcga aattggtgta gtacatgcgt tgccaaccaa atacccttat gatccagaaa
     7741 atccagcaga tgttcgtgcc gctgagttag aagatatcat ccataacaaa tttatcttgg
     7801 atgcaaccta ccttggacac tattcagata aaacgatgga aggggtcaac cacatcctag
     7861 ctgaaaatgg tggagaactt gatcttcgtg atgaagactt ccaagcactt gaagcagcta
     7921 aagatttgaa cgatttcctt ggtatcaact actacatgag tgattggatg caagcctttg
     7981 atggtgagac tgaaatcatt cacaatggta agggtgaaaa aggaagttct aagtaccaga
     8041 ttaaaggtgt tggtcgccga atcgcaccag attatgtgcc tagaacggat tgggactgga
     8101 ttatttatcc tgaaggcttg tatgaccaaa tcatgcgatt gaaaaatgat tatccaaact
     8161 acaagaagat ttacatcact gaaaacggtc ttggatacaa agatgagttt gtagataata
     8221 ccgtttatga tgatggtcgt attgattacg tgaagaaaca cttggaagtt ttatcagacg
     8281 caattgcgga tggtgcaaat gttaaaggtt acttcatctg gtcacttatg gatgtcttct
     8341 catggtcaaa cggatacgaa aaacgttatg gactattcta tgtagacttt gacacgcaag
     8401 aacgctatcc taagaaatca gctcattggt ataagaaagt agcagaaact caagtgatag
     8461 agtaatatta cttgaaaatg taaaaaattg ttatccgata ctttagaaaa agtacaacac
     8521 ttatttagac caggctttga aaaggtatat attgttagtt tcgaagattg tcctatgatt
     8581 cctgagcttg aagctaccct cgttgctaaa atgtggtaaa tggtatgttt taactggaaa
     8641 agagtggatt tgccattctg atttagagtt atcagcattt gaatgggaat ttttacaatc
     8701 actcgatgta gaaatacgtg agactattca cttcgaggta aattatttgc ctttccaata
     8761 ataaaaagaa aggaattagt tgtgactatc gaattaaaaa acgagtatct tactgttcag
     8821 tttaaaacac ttggtggtca actgacttcg attaaagacg aggatgggat agagtatctc
     8881 tggcaagctg atccaaatta ttggaatgga caggcgccta ttctatttcc tatctgtggt
     8941 agtttacgaa atgattgggc tatttataga cctcaagaaa gacctttttt tacaggtctt
     9001 attcgtagac atggttttgt tcgaaaagaa gaatttattc tggaagaagt caatgataat
     9061 agcgtgacct ttagtattaa accaaatgct gagatgcttg ataattacct atatcagttt
     9121 gaactgagag ttgtttatac cttgaatggt aaatcaatta gaaccgaatt tcaggtaacg
     9181 aacttggaaa tggaaaaaac aatgccttac tttattggag cccatccagc attcaatagt
     9241 tctttagtag aaggtgaaaa ctatgaggat tattatattg aatttagtga ggttgagtct
     9301 tgctcgatac caaagagttt tcctgagaca ggattattag atttgcaaga tcggacacct
     9361 tttttggaaa atcaaaaaat tctcgatttg gactactcac ttttttccca tgatgccatt
     9421 acacttgacc agctcaagtc tcgtagcgta acgcttcggt caaggaagtc tggtaaaggt
     9481 cttcgagtag attttgatga ttttccgaac ttgattctgt ggtctacaac taataaaagt
     9541 ccattcattg ctttggaacc ttggagtggt ctctccactt cacttgaaga aggtaatttc
     9601 ttagaagata aaagacaggt aacgaaaatt tcacctcaag aaacttctag aaaaatctat
     9661 gatattacta tccttaatta aaatttgtta ttatttgagt cttcaataag atttgaatac
     9721 tcttaccttg tgatgataga ttggtcttct ccgtgccagt cttacatcgc aaggttttta
     9781 agttttttgt gggtctatgt caactgtagt gggtgacgaa aagctaatat ctaaagacag
     9841 tctggtagta aaaaataaga ttatttacta cttcttgatt taaaataata gtggagccat
     9901 taatttgtgg caaatttaat ttaatatttt gcctcaaatt agccacactt tttatagaac
     9961 taagaggaaa aatgaaattt tcttcctatt aatatagttg tttgaaatct taaatatagc
    10021 gt
//
LOCUS       DAJSKS010000103         9914 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJSKS010000103 DAJSKS010000000
VERSION     DAJSKS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.105"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            79..513
                     /locus_tag="ON396_002102"
     CDS             79..513
                     /locus_tag="ON396_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3114172.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(591..1124)
                     /locus_tag="ON396_002103"
     CDS             complement(591..1124)
                     /locus_tag="ON396_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCR3114173.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1218..2396)
                     /gene="mutY"
                     /locus_tag="ON396_002104"
     CDS             complement(1218..2396)
                     /gene="mutY"
                     /locus_tag="ON396_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR3114174.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2389..3186)
                     /gene="recX"
                     /locus_tag="ON396_002105"
     CDS             complement(2389..3186)
                     /gene="recX"
                     /locus_tag="ON396_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR3114175.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3293..4675
                     /gene="rlmD"
                     /locus_tag="ON396_002106"
     CDS             3293..4675
                     /gene="rlmD"
                     /locus_tag="ON396_002106"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR3114176.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4849..5427)
                     /locus_tag="ON396_002107"
     CDS             complement(4849..5427)
                     /locus_tag="ON396_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HCR3114177.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5749..6219
                     /locus_tag="ON396_002108"
     CDS             5749..6219
                     /locus_tag="ON396_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HCR3114178.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6327..7553)
                     /locus_tag="ON396_002109"
     CDS             complement(6327..7553)
                     /locus_tag="ON396_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR3114179.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(8307..8507)
                     /locus_tag="ON396_002110"
     CDS             complement(8307..8507)
                     /locus_tag="ON396_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3114180.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8641..9249)
                     /locus_tag="ON396_002111"
     CDS             complement(8641..9249)
                     /locus_tag="ON396_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCR3114181.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(9334..9804)
                     /locus_tag="ON396_002112"
     CDS             complement(9334..9804)
                     /locus_tag="ON396_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR3114182.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
BASE COUNT         2954 a         1973 c         1643 g         3344 t
ORIGIN      
        1 ttttattttt gaaaacttgc catacttttt atttgtttta ttttccagta tactatacga
       61 aaggaaggtg gctagaccat gaaagttgta caaacaaaag atacaatgag caatatttac
      121 ttagatgctg tacggatcag gcatcaagtt tttgtagtcg aacagggtgt gcctttgtct
      181 cgagaaatag acaaagatga agcccattgt atccacttcg tcctgtactc agataaaaaa
      241 gaaccgcaag gaactgtacg tcttttgcct ttagaaaacg gaaaaatgaa attacagcgg
      301 atggcgattc tttctgaata cagacaccaa gggttaggaa aaatattgat tgaagaagca
      361 gaaaactttg ctaagaatca aggctataat acgattttat taggggcaca gtccacagct
      421 gaaacttttt acgaaaaact aggatataca gcttacggcg atccttttga agatgctggc
      481 atgcctcata tttatatgaa aaagacttta taaaaattta tttcgaaaaa agcaaaaaac
      541 agcaagcttt tgagaaaaat ctctcactgg cttactgttc ttttttgtgt ttacttacgc
      601 gacaattgct tgtaacggtt ataccaaata tcaatatagc cttccgagaa cgggccatct
      661 ccattattga tccagtcaac cagaatcttc acattttctt ttaggatgaa gtcaatatcg
      721 ttcgggtaat tcatcatttt gctgtgcatc tcatattcat cgacatccaa tagtcgtttc
      781 tcgccgtcag gaaatacctt gatatccaag tcatagtcga tatatttcaa ggcctcatcg
      841 tccagcaaaa aaggtgaagc tagattacaa taataggaaa cccccttttc tctgatcatt
      901 gctattacat tgaaccagta ttttttatga aaataaacaa tcgctggttc gcgcgttacc
      961 catcggcgtc catcagattc agtgactaat gtgtgatcat tcacgccgat catagagtgt
     1021 tcgcttgttt ttaataccat ggtatctcgc cacgttcgat gcaatttgcc gtcgtgcttg
     1081 taactttgga tcgtcacaaa ctctccttct tttggaactc ccatagcaac ccttccttaa
     1141 gcagcatttt gtgctttgtg tttgggtgtt cattacaccg acatcataat tattatatca
     1201 ataaaaatga gctttgccta gaaaggattc ctattgtttc gatttttctc taactgatct
     1261 accagtttca tttggacctt aggaaaaacg accgaatcaa atgcatcagg cttcaaccac
     1321 ttcgaagttt tgttctcggt aaattcttgt tctgctccct cggttgcccg tccgtaaaaa
     1381 aggagaacat gccatttcag atgactaaaa atatgagtaa cttctcctaa atgtcgtgtc
     1441 tgccaaacaa agagttcctg cttttcaaat gggagatttt gctcttcttc tgctactagg
     1501 tcatcaaaca gatcgatttc tggctttgtt tcccattctt ttttcaatcg ctcatactcc
     1561 tcttgtgtca cttccatcat tggaaatgtc cacatattcg ccagcaattt ttggctatct
     1621 ctttcttcaa aataatacgc tccagaatga ttttgaagag ctgcactgat ataataaacg
     1681 tcttttggct ttgctttttt tgtttttaca ggaaaactcg tttggattcc tcgtttgtta
     1741 gctaaacaaa aggcttggat cggacaagtc tcacactttg gagaagttgg tgtacagata
     1801 gctgaaccta gatccatcat tgcttggttg aattcacctg gatgcttttc atcaatgatt
     1861 ttccgcatcg cttcatcaaa gattttcctg ctagaagctt ttgcgatgtc tgcttcaatg
     1921 caaaacaatc tgctgactac tcgcatcacg ttgccgtcaa ctgcaggttc tggaagtcca
     1981 aatgcgatac ttgcaatcgc gcctgtcgta tatggtccta ttcccttcaa tgagctaatt
     2041 tcttcaggcg tttgcggcat ttccccatca aattctgaca tgatctgttt agcagcagct
     2101 tggatgttcc gggctcttga gtaataacca aggccctccc atgcttttaa aagcttttct
     2161 tctggagcat ttgctagctc ttcaattgta ggaaaccatt ccatgaaacg ataaaaataa
     2221 tcgatcactg tgtctactct ggtttgctga agcatgatct cagagatcca gatacgataa
     2281 ggatcacgat tatagcgcca aggcaggttt cttttttctt gttcatacca ttgaatgaac
     2341 tgatcttgaa attcttttgt ttctttatct gtccattttt cccattcttt attcactttc
     2401 ctcttccttt tccgtaatga atcgctggat catatccaaa tcaaatcctt tttgatataa
     2461 gctttgcttt acttttagat ttcttttttt aggttcaaac cttaaatttc ttcgccatag
     2521 tttatctcct tgtttgacta aataatcata ttctatttct tgatctgctt cttgcggaag
     2581 atcagctatc gcttgttgga tcacttcttg agaaaagcct ttcgtcatca atgtttgttg
     2641 gatttttctc aataattctt tttggctttt tccgtgattt tttccaaacg ctttttcggc
     2701 tgtctgatgt gcattttgaa cttggtcttc aaatgtatat tggctgagcg cttgttcaat
     2761 gatttcaggt ttcaaacctt tttgctgcat ctgctgcgct aatttctttg gtcctttgtc
     2821 acttaagcgc atattcgtgc gcacataact ttccccatag accaaatcat cgacaaggtc
     2881 caattctttt aagcgggcta cgattttatt tcgatcttct tgaaggattt ccttttcttt
     2941 taagtatgtt cggatttctt tttccgatcg caactgataa cttaagtaat tcattgctag
     3001 gtgaaaaccg aaatcagctc cgctactctt cttgatctct tggatagttt cttcgtctaa
     3061 ttcccgtcct tttaataatc gatagcgtac taatacatct tcagatactt gtaaagcttc
     3121 tccttcagat aaatctacgc gatagaaagg gcctttgcct ttactgattc tcgtaacggt
     3181 taacataaac atcctcctcg aacatatatt ctctatcata ccacagaaaa tccccgtaat
     3241 caggtattat atgttagaat aaaccacaga aaaaagaaaa agggagatac ttatggcaga
     3301 aacaattgtc aaaactggac aaacggttcc tttgaaaatc aagcgtttag gcataaatgg
     3361 tgaaggaatc ggttatttca aacggttgat catatttgtt ccatatgcac taccaaaaga
     3421 agaagtacta gtgaaaatca cgaaagcaac tcctcgctat gcagaaggag aattaataaa
     3481 agtcaaaaaa acaagcaaag atcgcgtagt ggcaccatgt cctgtttact atgaatgcgg
     3541 tggctgtcaa ttacaacact tagcttatca agctcaattg gattttaaaa aggatctgct
     3601 tttgcaagca ttggaaaaat tcaaaccagc aggctttcga tcttatcaac ttttgccgac
     3661 gatcgggatg gatgagcctt ggcattatag aaacaaagca caatttcagt tacgaaaaaa
     3721 taagcagact caaaaaattg aagctggttt atacgaagca aactctcatc agttagtccc
     3781 tataacagac tgcttggttc aagaacctcg aacacaaaaa gtaatgaata ctgttgttca
     3841 actattgaat aaatttgatg cacccatcta tgatgaacgg acaaacagtg gaatcttccg
     3901 cacaatcatg gttagaatcg gtgttaaaac tggggagtta caagttgttt ttattactcg
     3961 cagtaaaaaa ttcccgcaaa aaaacgcaat gatccgtgag atcaatgaac gactgcccga
     4021 agttgtttca atcatgcaaa atgtccaggc acaaaaaacg tctctcgtga tgggggatga
     4081 aacgatccac ttatggggaa aagaagccat tgaagaacaa atcaatgaag tgactttcga
     4141 tctttctcct cgtgccttct ttcaactcaa tcctcagcag accgaattgc tttatggcga
     4201 aggaatcaaa gcattagatg tccagcctaa tgaaacgatc gtggatgctt attgcggagt
     4261 tgggacgatc ggcttaagtg tagcaaaaca agccaaagaa gtacggggaa tggataccgt
     4321 tcctcaagca atcgctgatg caaaaaaaaa tgcggaaaga ttaggatatc acaacactcg
     4381 atatgaagtc gggacagcag agcaagttct gcctaaatgg ttgaatgaag gttttcagcc
     4441 tgatggtatc atcgtagatc caccacgtac gggtttggat aacgcattga tccaatcatt
     4501 attgaaatac cctccaaaaa aacttgttta tatctcctgt aatgtttcta cacttgccag
     4561 agatctggtt aatttaagta aaaagttcaa gattgaatat ttacagtccg tcgacatgtt
     4621 tcctcagact gcacgttgtg aagttgtcgt gaagatgacg agactcgctg actaaatgaa
     4681 agaggttgtg aaaaagtctg ccctgtatca agtaataaga acagccaaac aaaaatagct
     4741 cttcatgttt tatgaatact gtacttgtga ccgaagagtt aacaggagga cactatttat
     4801 ttaaaaaaag ggttgtgaca tagtttttgt cacagccctt tttgattatt attggatatt
     4861 cacgtctatc acttgataaa atgcatttgt cgtatcgctg atattccata ctgctaaaat
     4921 cacattatat ccttttcgat cctgcggaag tgtgatcgtt tggttgactg tttttggtgg
     4981 aacagttgct ttgtcgtcta tttttgtgat caattcaaaa tcactaaatt tcaaaggttg
     5041 attaggattc caagttggtt tggtaatgta gtaatcccaa gtacttgtcc gatgttgtgc
     5101 tgttaaattc caagtaatat ttaatgttcc tggattaacc atgtttttat gccatctgtt
     5161 agatgtctgt tcatctaaag gctcaaagcc gctgactcct gcactagcaa tttttccatc
     5221 aatgaatgta tttttggtag cttcgatact ttgcggttcc cattgtgctc ttccgacatt
     5281 ttgatttaag tttcctgctg ctgatgttcc taggtatgca cggcttcctg gagaagtaat
     5341 ataaccatgc gcaaaacctg gaatgctaaa accgaacaac cctaaactaa ctaacccaat
     5401 aaacaataac gtactaatct ttttcatgta gaaacctcct tcatttgata accacattgt
     5461 aaacgcttta ttgtattaac acaaacatta tgcttttata attttatgtt gaagtaaaaa
     5521 aggtgaaaaa agtaagatgc agattataac agaagtagac gagaaaagta caaatagaaa
     5581 acatgagaat caaatcatat gatctttttc aaaaacatca ttcatataaa caaatgttca
     5641 actttttaat aacaacaatt atcaattttt ttcttacttc acaaactaaa tacaggtctt
     5701 gctgtgagcc ttcatggtaa actaatatta tagaaaggag ttagtaaaat gaagatgata
     5761 aaagatattc gtttttgggt ttgtgttata ggaattgtca tattaggctt tttatcaggg
     5821 ttgcttagcg gaaatcccgg aaaatattac tattctttgc aacttccacc atttgcacca
     5881 cctagctgga tatttgggcc gatgtggacc ttactgtata tattgatggg tatttctctt
     5941 tatttgttac ttaatcacaa caacaaaaaa cagcgaaaca atcttgtcgg tctttttgtg
     6001 attcagttca tttttaattt tatatggtct gctctatttt ttaatttaag aaatattttc
     6061 attgcagcaa tcgatattac acttttagtt attttcttgt cagtgcttat gtatcagttg
     6121 tggctgcatc atcgcctggc aatgtggctc atgattcctt attatttatg ggtactattc
     6181 gcaactctat tgaattattc aatttatttt ttaaactaag cgctaaaaag agacgtcctg
     6241 taatttaaca catagaaaaa gtgccaccta agttagattt tttgtctaat ttaggtggca
     6301 ctctttatta accaaactat ttttttctag tacccacttg ttgttccata ctcattattc
     6361 tggtcataac cataaccatt ttgttcctgt ccagcgtaag gatcataact ttgagtatct
     6421 tgacttggtg tatagtactg acttgtatct tctgtttgct gataataggt attcggatct
     6481 tggtactgat catatgtgtt ttctgaatag gaagaataat cggtgtaccc ttcactattc
     6541 gatccatcac cgtttgtcga atcgccatct tccgaatcat caaagaacat cattgaagca
     6601 ttctcatttt tcaaattagg taatgtatcg cttgttttta tatttaattg tttcttcagt
     6661 tcattttgga tcgaaagaag atcatttttt cctaaaattt gatagtagat atcattgatc
     6721 atttgtcctt cgccttgcaa ctggtcttgt ttgattgttt caaacgccgg ggtgtagttc
     6781 gtcgctacat caagcatgtc atcccaatct aagtccgtcg tcacattgtt ctcaactgct
     6841 tttaaaattt ttctataatt gcttacgcca tctaaactta aaactttatt gacgattttc
     6901 gtcactactt cccgctgacg acgttgtcga ccgatatcgc cttctggatc ttcgtgtcgc
     6961 attcgagaat aggctaaacc tttttctcca ttcaatgttt gttcacctac tggtacatgg
     7021 acaccatcca aagtaaagtc aattttatta ttcactttga tgccaccgac cgcattgatc
     7081 aaatcttcca tgccatccaa attgatcgtt acataatgat caataggaat atcgagcagt
     7141 tgctcaattg aatccatggc catttcgaca ccaccaaatg cgtaggcatg atttaattta
     7201 tctaccgttc cataacctac gatattcgta taaatatctc ggtccaaact gatgatcgtt
     7261 gatttttttt gtttcggatt aactgttact accatcatcg tatcagaacg tccttgttcc
     7321 gttcttccta atccaccggt atctaaacct aatagcaaaa ccgaaaaagg ttctttgtca
     7381 tcaatgctta ctctctcaga acgtttggag gatattcgat tagagttttt agaaattttg
     7441 tttactgttt ggctcgcttc tcccataaat ctgattccgt atgctgataa tccggcaatc
     7501 agcaaaacga atacccctaa caaggttaat agaacttttt gccatcgctt cataatacac
     7561 ctctgtttca cattttctaa aatattgtct catataactg ttcaatcagc aaattcactt
     7621 tttaatcata agaaaacttt aaaaaacatt tttgtgtaac gattattatt ttttaaaatt
     7681 tctcaaaatt cttaactatc ataccatacc gtgaaaaaga tcgaaagacc gaatacacgc
     7741 agttcgcaat cgttttatct ttttttatgt attttttcca gaaaaagcga acaacaaggt
     7801 attttccaaa taatcgtgca gtaaaatgat cgcacatacc tttcccatct ttttaacagt
     7861 ctttgcctac cgttcagatt atcattcgat ttcacgacaa tttcttccta tgactggtcc
     7921 ttttgttaaa cccatcttct attcgtttta tttccagctc actgcatggc tcttaaaatg
     7981 actcgaacta gtgattgcat taccatcatc ttttaatgga tagatatgct cctcttcaaa
     8041 gtaaaacgat tttcttttaa agtaatagaa ttgacatggt aatgagtatg tatactagtg
     8101 gtttcacaaa tggtctttta ttcaacatcc aaaaactgaa atttctttaa aatccattac
     8161 taacgaagaa aatgttcctt gcttgataat tagttaggac atgtcaagtg aacaaatgca
     8221 tttacttagc caaaaaatct aaaaacttca taaatagaca aaaagacacg actctctaag
     8281 aagtcgtgtc tttattcaac aaattattat aatttagtca cttcagcagc ttgaggtccg
     8341 cgtgcacctt caacaatcgt gaattctact tcttgacctt cttctaggct cttgaagccg
     8401 tctccttgaa ttgctgagaa atgaacaaat acgtcatctc cgccgtctac tgtaataaac
     8461 ccaaatcctt tttcattgtt gaaccattta accgttccgt gttccataaa tgtatatcct
     8521 ccaaaaaagt tcttgcaagt aaccttgcaa gaacattata cccaatttag aaaacgctag
     8581 caagctttcg ctcttttatt ttccaaatta tttttaagac tgatatgacg gaggaaatgg
     8641 ttattttgct gcttcgaaac gtttgtttac ttcatcccaa ttaataacat tccagaatgc
     8701 tgcaatataa tctggtcgga catttttata tttcaaataa taagcatgtt cccaaacgtc
     8761 caaacctaga attggtgtct ttccttccat caatggagaa tcttgatttg cagtagaggt
     8821 aatagctaat tttccatttt ccattacaag ccaagcccat ccagaaccaa atcgtccagc
     8881 ggctgctttt ttgaattctt ctttaaaaga agaaaaatca ccaaaagctt cattaatcgc
     8941 ttcttttatt tctcctgtag gttcgccacc agcatttggt gccatgattt cccagaaaaa
     9001 tgaatggtta gcatgtccgc caccattgtt acgtacagct gtcttgatat ctgttggaat
     9061 agcgtccata tcagataata attcttctat tgttttttcg cctaattctg ggtatttctc
     9121 aattgctgaa tttaaattcg tcacataagt attgtgatgc ttatcatgat gcagatgcat
     9181 cgtttcttcg tcaatataag gttccaatgc gtcatatgca taaggtagat ctggtaatgt
     9241 gtaagtcata aaaaaattcc tcctcaatgt tttcttaata atgtctacat tacaaagagt
     9301 aacaaagaga aggaaattct tcaattgtta tgcctagtcg tctttttcac tttgacctac
     9361 ttcttcttca aaaacctcag tttctttaaa aataaacaac ttgctgaaaa tatagttcgc
     9421 aaggataaca ataatttgag tgatgatttt cgccacatac tcattgctgt tcataacatt
     9481 gatcaacaat accattgccc ccatgtccaa tccgtaagaa aggatacggt agaagaagaa
     9541 cttaccgcat tcaataagtg catgtgtaaa attcggtgat tttgaatgga atacccaaag
     9601 tttgttcgtt acaaaagcaa acaatacaga acaaaaccac gaaatagaat tggataaggc
     9661 catacttaaa tcaaaatatt ggcgtgcgag agcaaaacta acaaaattta caatcgtagc
     9721 caaaccacca aaaaagagat agactagtat ctcccaatat ccttttgctt ctaaatatcc
     9781 ttttaactgt cgatactgct tcatgagcca acctacctta ttaaaaattc ttatttatca
     9841 tactctatat ttcaataact ttcatcttta tttaaaaaaa ttaatttttt ttgatccttt
     9901 gcctttgtct ctgc
//
LOCUS       DAJSKS010000104         9813 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJSKS010000104 DAJSKS010000000
VERSION     DAJSKS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.106"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            80..817
                     /locus_tag="ON396_002113"
     CDS             80..817
                     /locus_tag="ON396_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3114183.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            831..1646
                     /locus_tag="ON396_002114"
     CDS             831..1646
                     /locus_tag="ON396_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR3114184.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1659..2306
                     /locus_tag="ON396_002115"
     CDS             1659..2306
                     /locus_tag="ON396_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3114185.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2322..2978
                     /locus_tag="ON396_002116"
     CDS             2322..2978
                     /locus_tag="ON396_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR3114186.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3140..3961
                     /gene="racE"
                     /locus_tag="ON396_002117"
     CDS             3140..3961
                     /gene="racE"
                     /locus_tag="ON396_002117"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR3114187.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3964..5319
                     /gene="rph"
                     /locus_tag="ON396_002118"
     CDS             3964..5319
                     /gene="rph"
                     /locus_tag="ON396_002118"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR3114188.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5320..5838
                     /locus_tag="ON396_002119"
     CDS             5320..5838
                     /locus_tag="ON396_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR3114189.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5945..6445
                     /locus_tag="ON396_002120"
     CDS             5945..6445
                     /locus_tag="ON396_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR3114190.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6725..7645)
                     /locus_tag="ON396_002121"
     CDS             complement(6725..7645)
                     /locus_tag="ON396_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCR3114191.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            7810..8823
                     /locus_tag="ON396_002122"
     CDS             7810..8823
                     /locus_tag="ON396_002122"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCR3114192.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(8933..9688)
                     /gene="fabI"
                     /locus_tag="ON396_002123"
     CDS             complement(8933..9688)
                     /gene="fabI"
                     /locus_tag="ON396_002123"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR3114193.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         3184 a         1714 c         2093 g         2822 t
ORIGIN      
        1 actaaactat cagatataat gtattttatt atacatcttt aaaagaaatc ttactaaata
       61 ataatgggag tgtaattcta tggcaatgat taaatttgat cacgtggata aatattatgg
      121 caagttccac gcattaaaaa atatcaatct cgaattcgag aaaggagaag ttgtcgttgt
      181 cattggtcct tctggttcag gaaagagcac gatgcttcgc tgtatcaatg gattagagac
      241 gatctcttca ggtaagctgc tgattaatga tactgacttg catgataaaa agacaaaact
      301 gacagaagtc cgaaaaaaca tcggcatggt tttccaacat ttcaatttgt acccaaataa
      361 aactgttctg gaaaacatca aattagcacc aatcaaagtg ttgaagcaag accaggcaac
      421 tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat atgttagata agaaagattc
      481 ttatccttcc atgttatccg ggggacaaca gcaacgggtg gcaatcgcca gaggtttagc
      541 gatgaatcct gagatgctgc tgtttgatga acctacttct gctttggatc cggaaatgat
      601 tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac ggtatgtcaa tgattgtcgt
      661 gacacatgaa atgggctttg ccaaagaagt ggcggatcgt gttatcttca tggctgatgg
      721 agaagtattg gaagacagca gaaatgtgag agactttttc gaaaatccaa atgaagtccg
      781 agcacagcaa tttatcagta aagttatcaa tcattgatag gaggaaaaag atgcgcaggt
      841 taaaaaaaat acgcctgatt ttccttttag gcattgcttt tcttgcacta gccggatgta
      901 aaggaaatgg gttggcaaat gaagatattt tagagcgaag caaatctacc aatgaaatca
      961 tctggggtgt caagtatgat actcgattat ttggaatgat ggatattgag agcagaactg
     1021 tacaaggatt tgatgtagat attgcaaaag caatcactaa aaagatcctt ggtgacaatg
     1081 gaaaaacaga atttgttgaa gtcacttcta aaactcggat tccattattg aaaaacggaa
     1141 atatcgatgc aattattgca acgatgacga tcacagatga acgaaaaaaa caggttgact
     1201 tttcagatgt ctatttcgat gctggccaag ctttgttggt aaagaaagga agccaaatca
     1261 aaagtgtaga tgatttgaat gcttctacaa cggttctagc agtcaaaggt tctacatcgg
     1321 ctgcgaatat ccgacagcat gcaccagatg caaaaatttt agaattagaa aactatgcag
     1381 aagcatttac tgctttacaa tctggtcagg gagacgcgat gactacggat aatgcgattt
     1441 tgttagggat tgcagatgaa aatccagagt atgagttagt tggtggaacg tttactaatg
     1501 agccttacgg gattgcgatc aacaaagggc aagaaaactt cttgaaagct gttaaccaag
     1561 cgctagagga aatgcatgca gatggcacat acgataaaat ctatcaaaaa tggtttccaa
     1621 atgaaactga aggaaaagta gagtaagggg gagaggcaat gagcgaattg atacaaactt
     1681 atggaccaat ctttgtcgac gggttcaaga tcacattgtt ttcgagtgtc ttagcacttg
     1741 tgtttagttt gattatcgga acgttaatgg caatcttcca attatcaaag aataaagtag
     1801 tgagcgcctt agcaaaggca tatgtagaat tttttagaaa cattccatta ttaatcatcg
     1861 tgatgttttt ttatgttgtc gtaccgattt attggtttaa tctagatgga ttccaagcag
     1921 gtacgattgg attaacgatc tatacatctg cttttattgc tgaaacagta cgttcaggga
     1981 ttcaaaccgt accaaaaggt caaacagaag caggactatc ttctggtttt acctattcag
     2041 aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat cgttatcccg ccattaggca
     2101 accaatttat caacctcgta aaaaactcat ctattttagc aatcgtagct ggtttagatt
     2161 tgatgtatca aggagacttg atcgctagtg aaacattcaa taccttcgat acgtatatca
     2221 tcgttgggtt attttactta gttatcacgc taccgttatc ttacttaatg gtttatcttg
     2281 agaaaaaatg ggcggttcgt tcatagaagg aggggaaata catggatttt agcggcgctt
     2341 tttcatggat gaatatccgt tttttattgg aaggattaaa ggttactgtt gaagtttcat
     2401 tgttttcgat catttttagt ttcttgatcg gtggtctgac aggtgtgatt cgttttgcga
     2461 atatcccttt cttttccaaa atccttggtg tagtgattga tattatacgg aatcttccat
     2521 tattattgat cattttcttc acttactttg ccttacctca aatcggcatt cagatgaaca
     2581 tcttctggtc cgctgtcgca gcattaacga tttttgagtc agctatgttg tcggaaattt
     2641 tccgagctgg actcaatgct gttccaaaag gacagatgga agcaggatta tcaacgggac
     2701 tgacttatgt agaatcgatg cgaacgatcg tgttaccgca agcatttaaa tcaatgatcc
     2761 cggcaattgt gagccaattg atttcactga tcaaagatac ttcattagca gtaatcattt
     2821 ctttacctga actttctcat caggcacgta ttatttatgg acagaacacc aattatgtgc
     2881 ttccgatgtt catcatcatg acacttatgt acttcgtggt atgttacgcg ctttctctgc
     2941 tttccggtta cttggaaaag agacggtaca gttattaata gatgattctt ttagcggttg
     3001 tggcaaacta aagtcacaac cgcttttcct ctttccattc gaaaaaaaga gatttcgatt
     3061 gttttaagca agtaagagca cttgaaaatt cacgctaaaa agtacacaat agggaggata
     3121 gaatttattg aaaaaaagga tgatacgatt gacagataat cgccctatcg gatttattga
     3181 ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg aaacaattac cgaatgaaaa
     3241 tattttattt gtaggagaca cagcacgctg cccatatggc cctagacccg cggaacaggt
     3301 aatccagtat acttgggaaa tgacggatta tctggtggag caaggaatca agatgctggt
     3361 gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa atcaaagctg ctctttctat
     3421 tccagtcatc ggtgtgatcc ttcccggtac tagagcggca gtaaaaaaaa cacaaaataa
     3481 acaagttggc attatcggta cgattggtac ggtaaaaagt caagcttatg aaaaagcact
     3541 gaaagagaaa gtaccagaat tgactgtgac aagtcttgct tgtcccaaat ttgtttcagt
     3601 tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa attgtggcag aaacattagc
     3661 tcctttaacc actaaaaaaa tcgatacatt gattttggga tgcacccatt atccattatt
     3721 acgccccatc attcaaaatg taatgggaga aaatgttcaa ctgatcgatt ctggagcaga
     3781 aacagtaggt gaagtatcta tgctgttaga ttatttcaat ttgagcaatt caccgcaaaa
     3841 tggtcggaca ttatgccagt tttatacaac tggctctgcc aaacttttcg aggaaatagc
     3901 tgaagactgg cttggaatcg gacacttaaa tgtagaacat atcgaattgg gaggaaaata
     3961 aggatgcgtc atgacgggag aaaagcccaa gagcttcgca aactggagct tcaaacaaat
     4021 gttttcaaat atccagaagg atcggtagtg atccgttttg gtgatacaac agttatttgt
     4081 tctgcaacaa tagaaaattc tgttccccct tttttgagag aaacaggaac aggatgggtg
     4141 acagcagaat atagtatgct gcctcgagca acgagtacga gaaatcgtag agaaagcagc
     4201 aaagggaaac tttctggtag aacaatggaa atccagcgtc ttattggtcg ttccttgcgt
     4261 gcagtagtag acttggaaaa attaggagaa cgtagtatca tcgtagactg cgatgtgatc
     4321 caagcggatg gcggtactag aacagcaagt atcactgggg catttgtggc attgaaatta
     4381 gccattgaaa aacttttaag agaaaaagaa ttgtcagaag atccaataaa agaacatctt
     4441 gctgcagtta gcgtaggaat cttaccagat gggacttgcg tcacagattt ggattaccaa
     4501 gaagattccg cagcacttgt agacatgaat cttgtaatga cagaatctgg aaaattcgtg
     4561 gagattcaag gaactggaga agaagcaacg tttgatggtg aacagttgaa tgaaatgctc
     4621 ttttttggaa aaaacgcgat tgaagatttg atcaaagagc aaaaacatgc tttgctaaca
     4681 gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa caatcatcat tgcgacaaga
     4741 aatccaggaa aagcagaaga gtttcgaaat atgttcaaag aagcgggata tcatgtaaaa
     4801 acattacttg actatcctga attgccagat gtggaagaaa ctggcagcac atttgaagaa
     4861 aatgcccgat taaaagcaga aacgattgca caattgcttg atcagccagt cttagcagat
     4921 gattcaggtt tgaaagtgga tgctttaggc ggtatgccag gaatctattc tgcacgtttt
     4981 gcaggggaac aaaaaagtga tgcggggaat aacgccaaat tattatatga attgacagat
     5041 gtgccggatg aaaaaagaac agcacaattc cattgtacgt tagtattcgc ggcacccaaa
     5101 aaagacagcc ttgtagtaga agcggaatgg cccggtcgag tagcccgtat accatctggc
     5161 gaaaatggct ttgggtatga tcctttattt atcccagaag gtaagaaaca aacagcagca
     5221 gaattatcga gcgaagaaaa aaacaagatc agtcatcggg ctcaagcaat gaaaaaattg
     5281 agtgccgagt ggaaacaatg gctggaagga gagagataga tgcggtattt agtagtaagt
     5341 gataatcatg gagacagaga tatcctcgtt gacttagtag atcgttatcg ttctgaagtg
     5401 gatatctttt tccattgtgg tgattcggaa ttagaagctt ctgacccatt atgggataca
     5461 ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat tcgaacagaa aaaagtgatc
     5521 caaactggtc aggatacagt attcatgact cacggtcatt tatcgaatgt ccgatttggt
     5581 ttgacacagt tagcaatcga agctcgagct gcaactgccg atatggtgtt ttttggtcat
     5641 acccatcaaa taggctgtga gatggatcag aaaatcctgt ttttgaatcc tggtagcatt
     5701 tctcagccta gaggcccgat ccaaatccca tcatatgctc ttatcgaaag tttagatgac
     5761 caaatttctg ttcaatacta taaccgtgcc catcaggcag ttgaagagtt ggccttttct
     5821 ttttcgagag agcgctgaat aaaaaaactc taatttttaa taaaaataag ctttatttta
     5881 tgaattttgc atccggtttc ggtaaaatag tagtgttgaa tgaaatagaa atggaggcca
     5941 tcagatgatt ggacctatag taagagaaat gctgctggaa aatcaggaaa cgtttttgat
     6001 tcctgcagaa aatgtggcaa atgttatgta tcatcatcca ttgtcccatg gtttacttgt
     6061 gttatcgaag gtcggttatt ccaaaattcc tgtattagga aaagatgatc actttgtagg
     6121 actagtaagt ttatcagatg tggtcaataa aatgattgac ttgcaaacaa tcagcatgga
     6181 cccgttagaa ggactaacgg ttgctgatgt gacagaaaca gatgttccga caattgatga
     6241 aaattgggaa ctagaagaag tactccattt gttagtcgat tcgccttttt taccggttgt
     6301 cacagaagat cggattttca aaggaatcat tacaagaaaa gagctgctta aagccgtaaa
     6361 cttcatggtg catgaattag aacgtcgtaa tatattgtct ccgaaaatcg aagaattaaa
     6421 agaacaaatc gatatcgtga gctaaagaac gagaaactaa aagataatca aaaaataagt
     6481 agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa gaggctggga caaaagcgtt
     6541 taactccaag aaataagaag gaattcacga aaattgcttt tcaaattttt gtgaatttca
     6601 gcttatttcc gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgggac
     6661 aaaagtgttc tttacttttg tcccacgctc tttttagtga gattcgttgc atcgtttgtc
     6721 caaattattt tccgacagca attggactgt tcggaatcgt gaaaccagaa gctgtcaatt
     6781 ctttgacata ggagctcaag aactcttctt ttaaggcata ttgttttccg ttcaatgcgt
     6841 agcagaccgt acgaatcgca aaattgctgt ttccaagatc cactaatcca aagatagaag
     6901 gttcagtttg gagctcttct tggtattttt cagctaatgt acgatttact cgatcaatga
     6961 tctcatagat tttgtcatat ccttcttcag gaacaatccg aatatctaaa agtacttgca
     7021 tatttgcacg agagagattg ctgatcgtgg tgatgtttcg attcgggatg aaatggacag
     7081 taccatccgt tgctttcagc tgaagggtac ggataccaac agatacgacg gttccttcaa
     7141 tcgttaagtt tgctagcttg atgtagtccc caacatccat ctgttgttcc atgataataa
     7201 aaaatccggt aataacatca ctcatgaacc cttgggcacc aagaccgatt gccacacctg
     7261 caattccggc accagccaaa agtgatccga tcggcacacc gatagcagat aaaattgcat
     7321 agataaaaaa gaaaccaagg gtataatgaa aaatagtatt taaaagacta tgtaaagtct
     7381 ttaagcggct ctcgtttaga atggcttttt tagaatactg attaaaagac ttatcaatca
     7441 gatattttcc cacacggtga agaatccaaa aaaggacgat caaaaaaatc agatataaac
     7501 ttttttggat aaaggcagcc aaaatggcgt cccaatcaat cccgtcccaa aaccgctgaa
     7561 acgcatttac tttgctgaca gcttgatctg tcaatgtctc gccagaagag tctgtcgtac
     7621 cggcagttgc taataatgaa aacataaaat cctcttttct aaaatttgat tctttctgta
     7681 ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata actataatga aggaaaaaag
     7741 ctgaaaataa aatgacaaat aatatggata atgataaaat gtggagaaaa taacgaaggt
     7801 ggtaagtata tgcaaatcga ttggaacaat ctaggttttt cttatatcaa aactccttgg
     7861 cgttttattg ccaaatggaa agacggacaa tgggaagaag gagaattgac agaagataat
     7921 tacctgacga ttcatgaagg ctctccagcg cttcattatg gtcagcaatg ttttgaaggt
     7981 ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct ttcgacctga acaaaatagt
     8041 cgacggatga atcaaagtgc tagacgattg ctgatgccaa tggtccctga aaagatgttc
     8101 gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt ttgtgccgcc ctatggaaca
     8161 ggcgctacat tatacttgcg ccctttactg attggggtcg gtgaaaacat tggtgtccat
     8221 ccagctccag aatatttatt tgtcgtcttc tgtacgccag taggagcgta tttcaaaggt
     8281 ggactaaaac caacgaattt tattgtatct gattacgatc gtgctgctcc taaggggact
     8341 ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac ttccaggaac agaagcaaaa
     8401 gaactggatt actcagattg tgtttatctt gatccagcca cacatacgaa aatcgaagaa
     8461 gtaggtgcag caaatttctt tggaattaca aaagacggaa aattcatcac tccgcaatcg
     8521 ccctctatct taccaagtat cacgaaatat tccttgttgc agttagctga agaacgacta
     8581 ggactgcaag caattgaagg agatattttc attgatgatc tagatcaatt tgcagaagct
     8641 ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg ggatttatca caataacaat
     8701 cttcatgtat tttatagtga aacggaagta ggaccagtaa caaaacggtt gtatgatgaa
     8761 ttgacaggta ttcaatttgg agacatagaa gcaccagaag gctggattga agtcgtggaa
     8821 taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa aaatcaggaa gagactttaa
     8881 aaagtttttt cctgattttt atatgtttta ctttaaagaa tgcaacaaaa gtttatgaaa
     8941 gatggacacc tttgtcaacg tagataatat caccgatgat tccgctggaa agtgggctga
     9001 ttaagaaagc acaagcatta ccgacttctt cgatcgtcac accttgctga tcaggcgtac
     9061 gagattcaga taactggatc agtttttgat agtctttaac acctgtgaca gctaatgttt
     9121 tgattgcccc agctgagatt ccattgaccc gaattccttt aggcgagaac tcagctgcca
     9181 aataacggat agcagcttca agagaagctt tcgcaatacc catcatgtta taattcggga
     9241 ttgcgcgttc tgaacctaga taagtcatgc taacgatccc gctgtttggt gcaagaaacg
     9301 gttgtgcgta ttttgataca gcaattaaag agtagctgct gatatcttga gctaattgat
     9361 agccgtctct ggaaatatca gacacatttc cggacaattc ttctttgttg gcgtaagcaa
     9421 ctgcatggac aaggccgtgg atctccccag catattcacc gatggcattc attgcttttt
     9481 cgatgctttc gtcgctagaa acatcacatt ctacagtgaa tgcttcttcg ccgaccaatt
     9541 tgactaatga ttttttcatt cgttcatttt ggtaagtata gataatttct gccccttgtt
     9601 tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt gttagcgacg cccataatga
     9661 cgattttttt tccttctaga aatgacataa tgactctcct ttgtttctga taaaaatttg
     9721 atagtcgtaa aaaaatagag aataatagct aaaataactt cttgatttct taattattag
     9781 tcaaaatgtt ttgattgtca aactattttt tct
//
LOCUS       DAJSKS010000105         9811 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJSKS010000105 DAJSKS010000000
VERSION     DAJSKS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.107"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(34..2154)
                     /locus_tag="ON396_002124"
     CDS             complement(34..2154)
                     /locus_tag="ON396_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR3114194.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="ON396_002125"
     CDS             2384..3562
                     /locus_tag="ON396_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCR3114195.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="ON396_002126"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="ON396_002126"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR3114196.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="ON396_002127"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="ON396_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCR3114197.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="ON396_002128"
     CDS             complement(4915..6168)
                     /locus_tag="ON396_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3114198.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="ON396_002129"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="ON396_002129"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR3114199.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="ON396_002130"
     CDS             7746..9092
                     /locus_tag="ON396_002130"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR3114200.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="ON396_002131"
     CDS             complement(9400..9786)
                     /locus_tag="ON396_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR3114201.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1782 c         2009 g         2892 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta t
//
LOCUS       DAJSKS010000106         9783 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJSKS010000106 DAJSKS010000000
VERSION     DAJSKS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.108"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            91..3369
                     /locus_tag="ON396_002132"
     CDS             91..3369
                     /locus_tag="ON396_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HCR3114202.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3910..5817)
                     /locus_tag="ON396_002133"
     CDS             complement(3910..5817)
                     /locus_tag="ON396_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HCR3114203.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5947..6822
                     /locus_tag="ON396_002134"
     CDS             5947..6822
                     /locus_tag="ON396_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HCR3114204.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6902..7876
                     /locus_tag="ON396_002135"
     CDS             6902..7876
                     /locus_tag="ON396_002135"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR3114205.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8056..9201
                     /locus_tag="ON396_002136"
     CDS             8056..9201
                     /locus_tag="ON396_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR3114206.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9338..9682
                     /locus_tag="ON396_002137"
     CDS             9338..9682
                     /locus_tag="ON396_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HCR3114207.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT         3187 a         1801 c         2063 g         2732 t
ORIGIN      
        1 ttattataat tttgtttttt attgacagta aaaataaatt aggtaatact ttaagtaaac
       61 gctttctaat ttgttacggg aggaatacac ttgttaaaaa aacgtataaa atacagtgcg
      121 ctaatgatga atctccttat gctcagtacc ccggttttaa tttctgtcgt tgaagttgct
      181 gctaacgaac aacagtctgt gaacgaagaa aatgcacacg tgaacattga agggatgtca
      241 acaatggagg aaataccaag aactagtgaa gatatggata cggaagaagg aaatgatcct
      301 atgatgaaag aatcagaaaa tgaaaagaca aaaaagcaat ctgaagaagc aaatgaagag
      361 tggaaagggg ttgttttcgg agaaagcaca agtgctagta ggtatgccat tgaaccatta
      421 gaagacggcg tgcggctctc ttccacgaat aacggcggaa aattccagtc aagcggatca
      481 gatggaatga cctattatta tcaagcaatc cccaaagaca tcaactttag gatgcgggcg
      541 acgatcgaaa tagaatcttg gacctatacg aatgcccaag aaggatttgc tttaatggta
      601 agagacgcag taccaaaaaa tcatttgttt gggcaggcat tttattcaaa ttcatttgcc
      661 atactaggca gtcggatcga atatgtctgg gactcggata gacaagaagt ggtcaacacg
      721 agcggcagca aatatacgat gcgacttgga ttggggacac gggcaatcac aggaatatca
      781 tctgaagatc cgcaagctgc gccagctcct gggagtgtct cagtggaaac aacaccttta
      841 gagacttcag cagggttctt ggaaaaagaa acaggttctt ataatatatt cggaaacggt
      901 catgggaatc tgtttccggc cacgaactcg attactaaac tggacgtaga gatgaaaaaa
      961 acgaatactg gtttagaggc atattattac gatcctgaaa caggagaaga aatggcttct
     1021 gacatcatgt atgactggga aaaattatac gcttcagatg aatcggttat ttatgcagga
     1081 tttgctgcag caaggaatat gacgatcaag gtagaaggaa tcgaagttgc ttactcagat
     1141 cctgcgacag atcctcctgg gcaagaaaga cctactcgct tgattgatcc gatttacact
     1201 gtgacctcta gtaatcattc aggaaaccaa gatcatacta tgtcctttag agcaaatgct
     1261 gacggattat tgacaattaa aaataaagca acaggagagg tgcttaagaa cgcaaaaaac
     1321 ttggctatca ctgcgaatcg ggaatttgat tatcagacaa aactgatgga aggaacgaac
     1381 gaatttactt tcagttttac gccagataaa aactatccgc cagaagaata cgtagagatg
     1441 actagttatg aaacaaaaga gattcttcat acagtggact accgtaaacc taaatattcg
     1501 acagtttatg taacaccaga agggtcagat gcaggaaacg gaagcagaca aaatccatta
     1561 tctctgacac aagcattgag atacgcatat gctgggcaga caatcgtcat ggcgcctgga
     1621 acttattcct tcgaaagagg attgacgatt ccaaaaggcg ttaacgggac aagagaaaac
     1681 ccgattcagt tgattgcaga gaaaaatcca gaaaacaagc gtcctgtact tgattttaaa
     1741 ggaactggaa atgggatgac actgttcagt cactactggg gtttacgagg gtttgacatt
     1801 acaaatagtg cagctatgca aaaaggatta cagatttctg gaaatcataa tttgatcgaa
     1861 gagattcatg cttatcggaa tggaaataca ggaatccaaa tcagcggtag ctcaaatgat
     1921 cctttcgaag catggcccgc gcataactta gtcaagaact gtacatcttt tgaaaatgcc
     1981 gatccaggat ttgaagatgc cgatggattt gcggccaaac tgacaatagg agaaggaaat
     2041 gtatttgacg gttgtatcgc gtatcataat gcagatgacg gttgggacct ttttgctaaa
     2101 aatgaaacag gttcgatcgg aaaagtaatc atcaaaaact cagttgctta tcgaaacggc
     2161 tgggtaccag gaattgaggg agaaggtaac ggtaatggat tcaaaatggg cggttcttct
     2221 ttaacgggcc ctcatgaatt gatcaatagt ttgtctttcg aaaatcttgc gaaaggaatc
     2281 gattccaata gcggaacaga tattatcgtc aatagcagta caagttttaa taacggttca
     2341 tataatgtgg ctctttatac ctcttcagca ggaaacacag actaccatgc ttctggtatc
     2401 ttatctttcc gaacagagaa tcttgagatg agagagcaga tccgcccatt gaatcaaaac
     2461 gaagatcaaa tctaccactc gttgaactac tattggaatg aacgagagaa acaatcagaa
     2521 aatactcttg gacataccat atcaaaagat tggatcgagt ctctaaatac atgttcgaaa
     2581 gaaggtcagc agcctgttac gcgctatgaa aatggaagta tccatgttca tggactgatg
     2641 cagattaaac cggggaatcg tcaaacagaa aaggaaagag tggatggtct tccattatct
     2701 gtaggtgcta tttttgacgg agaaacctca ccgtcttcgg aatatccaga gtatgcaatc
     2761 gtacaagaac caaatcagac aaaaccaaat gaacaaaata atccgattat tatacaagaa
     2821 gcaaatggtg tggcggagaa tgcagaagga aatatcattg aactgaagcc ttttacagtt
     2881 tttgtgtcaa ctttcacacc aaatcaagga acgcctgtct ttgaagatat gcgttatttc
     2941 ccagaaaaga tccaaatcgt tgaaacacag tcaagtgctc aattttctaa tcataccgct
     3001 attttttcag caccttcaaa aggagaatat acgctagaag tcacttatcg ttcggaaata
     3061 tttgatgggg aaaaatggtc tgctcataca gaatctgtta cccagcaaaa agcgatcact
     3121 gtgcgagaaa ctgccgatgt gcccccggga aatgacaaac cagaaagaga aaatccagga
     3181 aatgaatcac caaatggaaa accaaatgga aaaccttctc atccggatag gacagcaaaa
     3241 gaaagtgaaa aaaatggcac tcaaggactc ccaaaaacgg gagaatttaa taacccgttg
     3301 ctagcattag ctggaggaat tttgttaatc ggtgtagttg tttatgtcat gaaacagcga
     3361 aaaaaataag tggaatttct ccttttatga tgtaaaacaa ttgactgagg aaagtaaaaa
     3421 tagaaggaaa tgagaccaat agatgtctca cttccttcta tctttttata gatgaggttc
     3481 tccttgctgc ttttgattgg ctgttcttat tacttgatgc aggacagctt tttcacaatc
     3541 tctccactaa acggtgttca atcagctgac cctcggtatt agctcaacaa acggcaaaat
     3601 atgaggaact atttttccgt ttttttctct aatactcagg tcaggacgct gatttcacaa
     3661 cctctcgata aacggtgttc aaaagccagt ttctcggtag taagtcaaat ttatgcaaaa
     3721 atgtggaaag ccattttcac aaaatttttc ttactacttg aaactaagca gctttttcac
     3781 aacctctaat acatggtatt ccagaagtaa cttcttcgaa aaataagccg aaatgtcaca
     3841 aaaatttgag aagcgatttt cccaaattcc ttcttatttc ttgaagctga ccacttctgg
     3901 cacaacctct taccagattc gcactctttt ttctggcgct agatacatag cgtcttgttc
     3961 cgtgatacca aatgtttcat agaattcgtc aaggtttttt ggttgcacgt ttgctcggag
     4021 tttagcaggg gcatggacat cgatttgcag taaaagttgt tggtattctt tttttgcttt
     4081 tgtacgccaa atcgttgccc aattgacaaa gaattcctcg atagaaagat ctgattccgt
     4141 ttttgctgct tccaatgcac agcttagacc gcctgcatca gcaatatttt ccgaaacggt
     4201 taattttccg tttacttttc cgtcagcaaa aggcagacca tcgaattctt cgatcattgc
     4261 ttgtgctttg ttttgaaatt gtgtaagatc ttcttctgtc caccagttgt tcaagttgcc
     4321 atattcgtca aataaagcgc cgttattatc aaatgcatgg gaaatctcat gagcgataac
     4381 agctccgatt ccaccataat tagcagagct tgattgttct agactgtaaa atggtgcttg
     4441 aaggatcgct gccggaaaga cgatcacatt ccgaaatgga tgataataag cattgaccgt
     4501 gtcagcactc atttcccatt ctgaacgatc cactggtttg ttccatttgc tgaaacgatc
     4561 ttttaatgtg agtttgctga aaacaagagt atttgaaagc aaagtaccac cctgatctgc
     4621 aggtactgtt ttgaactggt cgaataatgc tgggatctta tcggggtagc ctacttggat
     4681 acctaagtta tttaatttta tgacggcttt ttcttttgtt gcatccccaa gccagtcatt
     4741 ttcttctaga cgttttttat aaacggcgat catttttttc accatttgtt ctacgtcttt
     4801 tttagcagct tcgccaaaat atttttttcc gtagtaatcg ccaactactt gatcgaattg
     4861 tccagaggca aggtaatacg ctgctttttt ctgaggcatt gcttcgtctg ttccagacaa
     4921 tacgcgagaa tagattccgc tgacttggcg gaattcttct gatagataac cactaagaga
     4981 acgtactgtt ttaatgatca tccagctttt gaataacgga aaatgagctt ctgtcaggat
     5041 gtcattcaaa tgatcgaagt acactggatc agttacgatc actttatctg gagttgtccc
     5101 aatcaaaccc gtgacaagtt ttttcaagtc cagtttatct gtgtatttaa taaatgtatc
     5161 aaaagattgc ggattataca ttttgctgta atctgcgttt tcttctgcac tttttacatg
     5221 aggagcaatc aatttatcaa attgcactgc ttgttccgtg attgcttctg cctgactttt
     5281 ttctttgccg gcaagctcta ataactgaac agccatatca aagaaaacgc ctattagttg
     5341 tgcaccgttg ggatggtctg atgcataata cgtcttatct ggaaggaata aagaaggcgg
     5401 ataagcaaag aaggcgttcg tttgcgcatt cttcatatct gcatctacat ccagattaaa
     5461 tggcaatggt aatgtatcca acagccattc tggaaattgc ttattcaatt cgtcgaaaga
     5521 ctgcaatgat tcaacttttt cgattatagg ttgaagcggt tgagcatgca actcatttct
     5581 tcggtcataa tcgtttgcca gttgataata tgcaaggaaa tgtttcattt tttctgaaga
     5641 cactttttct ggatgggcaa gcagatcgtc tgtttctccc atcaaaagtt ggtcgatccc
     5701 gtcaactaaa tcttggaaac cgcctgtagc tggtttatct gcagggattt ttgctgtttt
     5761 ttgccattct tcattgactg cttcatagaa atcttgtttc aatcgttctt tgttcattca
     5821 tcgatcgctc cttttttgtt tcgtgtgccc attatcttac aaaatgaagg aattaacaaa
     5881 tagaacataa aaaattgaaa attcgaacaa tcaaactgat ttgttttttt caatccttca
     5941 cttataatga aaaaaaggag gcgatcccaa ctgaatcaag tagtggataa acctttatat
     6001 ttcaaagtag ataaaaaaat taaaaaatta gccagcactc agcagctgca gtcaagaaaa
     6061 agtaaattgc tttttttagc tctggttttt gaagatcaaa gttatgtgat tatcgatcaa
     6121 tcaggacatc cggtagaata ttctccagca aaatatactt atcaagaggg tctttcttgt
     6181 aaaaaatgga agctgatcaa tgaagagcca atcgagttat ctagatggat caacagaaaa
     6241 gaagatattc ccgttttgat cgaagaaaaa aggtcaggaa aggaactcgc aaattgctgg
     6301 gtgggattgc cagaagaacg ttttttgagg tataagaaat gggctacccc ctcaggctat
     6361 ctttgtggta cttacgcggc agcagttctc cttgcctatt atcaagacta tcgtaagggg
     6421 tggatgcttc caaatgagat tcgcaagaaa aatacggctg attcgttggt tttgacgaag
     6481 gcattgcgta gccagatcca accactagga cttcctacga ttccttttca ggttagtaca
     6541 gggatttcta gttttttaaa gaagaatggc aatcatgagc gtgcacgagc aactcttcta
     6601 ggttcatggc agcgagctac aaaacgtata cgtgaaggca aacctgtgat gattggtatt
     6661 ttgaaagtac ttggcagtac atatggcaat cattgggtga cagcgtacgc ttattttgaa
     6721 actgaaacag gagaacgtta ttacaaagta cacgacaact ggggcgatta tcataaagtc
     6781 attcctgcca gttggagtaa cggaacagtt tcacttcctt aagaatttgc tgaaattaac
     6841 agaaaatctt ggaaaaatat gttaaactat cgaagaatag agaagttttg gggaggacta
     6901 catgagtaat aagtatcagg atgacacgtt gaagattttt agtttgaatg gcaaccgtcc
     6961 attagctgaa aaaatcgcga aagtgttcgg aacagaatta ggtaaaagcg tcgtgaagca
     7021 atttagtgat ggcgagattt ccatcaatat cgaagaaagt attcgagggg atcatgtgta
     7081 tatcgttcaa tcaacgaatg cacctgtcaa tgattactat atggaactac tgatcatgat
     7141 cgatgcaatg aagcgagcaa gtgcgaaaac gatcaatgtc gtattgcctt actatgggta
     7201 tgctaggcaa gaccgcacgg cgaaacctca tgaaccgatc actgcgaaat tgatcgctaa
     7261 cttgatcgaa gaagcagggg caactcgagt actgacttta gatctgcaca cggttcaagt
     7321 acaaggattc tttgatattc ctgtagataa tttatttacg atgccgttgt ttgctcatta
     7381 ttaccgtcaa ttaggtctga cgggagatga catcgtagtt gtttcgccaa aaaacagcgg
     7441 tgttcagcgt gcacgtagtc tttcagaata tttgaacagc acattagcca tcgtagatca
     7501 tgcagatgat gcttcaactg agggtggtta tgtgatcggt gatgttcaag gaaagacatg
     7561 tatcatggta gatgatatct tgaacacagg agcaacgctt gcacgagcag caaatgtatt
     7621 aaaagaaaac ggcgcaaaag aagtatatgc ctgtgcgtca catggattat tgtccgatca
     7681 tgcgaaaagt attttagaca atgcaccaat caaagatatt tgtatcactg attctgtttt
     7741 cacagaagaa aatcgtcatc cagaaaactt aacaatcgtt acatgttcag aattaatggg
     7801 tgaagcggtc aaacgcatcc atgaaaatac accgatgagt ccgttattcc gtctggaaga
     7861 aaaagaattc gaataaacaa aaaaaagagg gaaagaagtt gtgacagaag tgagcagctt
     7921 caaggagctg cgacaagact tttgtcacag ctcctttttt ctatgcccat gaaacgtcct
     7981 ctagacaaaa atgagctctt ctttggtttg ttcgctattt tatgctagaa tgtgcctagc
     8041 aataatttgg aagggatgaa tgaagtgaaa actatttatc tggatcatgc ggcaactacc
     8101 cccatgcatc cacaagtaat tgaagcgatg acggcaatca tgcaagacac gtttggaaat
     8161 ccttcaagca tacatggatt tggtcgccac gcgcatgaaa aattggaaac agcaagacaa
     8221 atcattgcag acagtctgca agcaaaacct cacgagatca tctttaacag tgggggaaca
     8281 gaaggggata atacagctat cttagaaaca gcgttctcgc gtcaaaaaga aggcagacat
     8341 ctaatcacta cagctatcga acatcctgct gttcttaata ccatgaaata tttagagaca
     8401 aaggggtttg acgttacata tctgcctgta gatgaaaaag ggaatctgtc gatcgaagag
     8461 gtcaaaaaag cgttacgaga agatacgatc ttggtctctg tgatgtacgg gaacaatgaa
     8521 atcggaaatt tgatgccaat caaagaaatc ggtgaacttt tagcggatca tccagcttat
     8581 ttccatacag atgcggtaca agcgtacgga aaccaaacga tccatccaga agaattagga
     8641 atcgatttat taagtatctc tgctcataaa atcaatgggc caaaaggcgt cggtttctta
     8701 tataaacgag acggagtaaa tatccctgca ttgcttttag gcggggagca ggaagaaaag
     8761 cggcgtgcag gtacagaaaa tttagcaggg atctggggta tggcaaaagc agttgaactg
     8821 ctgtcacctg aagaacgtga agcacgtaac aacaaatata aaggattcca gcaattgatt
     8881 cttgatacat tagaagcaaa cgaaatccct tatcaagtaa atggtgattt aacgaataaa
     8941 ctccctcatg tgttgaatct tcgcttaatt ggcgaagcaa atgatttgct actgatgaag
     9001 cttgatctta atggggtcgc tatttctact ggttcagctt gtacagcagg aaatatcgag
     9061 ccttcacacg tattagaagc aatgtatgga gaagatacac cgatactaaa agaatccatc
     9121 cgtatcagct ttggtttggg aaataccgaa gacgacatcc agtatttcct tgatcaatta
     9181 gttcgtgctg tcaaaaaata actagtgaag tcattcttat atgaacagat gtctgagaga
     9241 acgccttcat tttttaggga acttaactga aaaacctagt aaaaatcagg ataaactttt
     9301 ataataaaga gaaactaatt ttatcgaggt gaaaatcatg gcatttgaaa caacagcaac
     9361 ggtactgggc gcaccaatta gctatcgttt gcatcctgat gcaaaaagat atacattgcg
     9421 cgataatgga tttacagaaa caaacggagg caattatcaa ttgatccgac cattagatgc
     9481 cacgccgcaa agcaaagaag gattcaaatt aaaaatcact gtatcaaaag atattcaaac
     9541 attgaaaatg tcgatcacta cagcaaatgg attgaaagca gtcaatattt tcaaagatcc
     9601 aaaacagaaa atgagccaag ataaatttta ttttctgatg gatgggatga ttagccgtgg
     9661 gttattcgaa aaagtcgaat gatctgtgga tataaaaaga caactagttt tgaaacggcc
     9721 tattgttaga tgactaactt tgtaaggacg acgcaaatta gttgtctttt ttatttttaa
     9781 att
//
LOCUS       DAJSKS010000107         9648 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJSKS010000107 DAJSKS010000000
VERSION     DAJSKS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.109"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            569..1723
                     /locus_tag="ON396_002138"
     CDS             569..1723
                     /locus_tag="ON396_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HCR3114208.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1717..2139
                     /locus_tag="ON396_002139"
     CDS             1717..2139
                     /locus_tag="ON396_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114209.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2441..2821
                     /locus_tag="ON396_002140"
     CDS             2441..2821
                     /locus_tag="ON396_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR3114210.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            2803..3717
                     /locus_tag="ON396_002141"
     CDS             2803..3717
                     /locus_tag="ON396_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR3114211.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            3957..4646
                     /locus_tag="ON396_002142"
     CDS             3957..4646
                     /locus_tag="ON396_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114212.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQVLAQKIEKFTA
                     CYEDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKV
                     EEACSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4766..4852)
                     /locus_tag="ON396_002143"
     CDS             complement(4766..4852)
                     /locus_tag="ON396_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCR3114213.1"
                     /translation="MFELFICPLLVGVCISVFSYWLNNRNKK"
     gene            5498..6430
                     /locus_tag="ON396_002144"
     CDS             5498..6430
                     /locus_tag="ON396_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR3114214.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIH
                     GEVDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEAD
                     FKKQQQTEKEEAEKQSISNELKQDLLENLQNLFD"
     gene            6447..6917
                     /locus_tag="ON396_002145"
     CDS             6447..6917
                     /locus_tag="ON396_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCR3114215.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTL
                     VVSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLD
                     QQQQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
     gene            7001..7305
                     /locus_tag="ON396_002146"
                     /pseudo
     CDS             7001..7305
                     /locus_tag="ON396_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7580..8761
                     /locus_tag="ON396_002147"
     CDS             7580..8761
                     /locus_tag="ON396_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR3114216.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKI
                     TKELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVL
                     TGAKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIAT
                     LPIGILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISD
                     TGRDFLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNI
                     HVPVRESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVA
                     MIFFHVFENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQ
                     K"
     gene            complement(8977..>9648)
                     /locus_tag="ON396_002148"
     CDS             complement(8977..>9648)
                     /locus_tag="ON396_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3114217.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT         3519 a         1576 c         1584 g         2969 t
ORIGIN      
        1 aaaactgtgt tggtactggg ttctatttct ctaggaatat taggttttag ccccatgcta
       61 ttatccgtaa ttggaaaaaa caattccaac agagaactta aattaaattc aaattattac
      121 aacagtccta acgaagaatt gtttgtaaaa caaacggata aataataaaa aagactgctc
      181 tttcgagcag ccagttaaaa aataatgagt gtttattctt gaaaaagcac gcgcttatga
      241 agcgctacca aaaatttacc atattttaca aaaaataaca agtcttaaaa aaatgacaga
      301 taaaaatgca caaagaatta gatatagata accgcgaaaa atcaagcttt agaaagttgc
      361 ttttagctag accaattaaa aatcacaaag tgagcgtctc tatccattgg tagaggggct
      421 acttggcata gtgtacatta tgttaactaa cgctataaat aacgattatg ttcccaaaac
      481 aatattttat gaactataac aaaaatttgt tatcgcatat tgtatatttt aattataggt
      541 gtataccact taaatttaac acaaaggcgt gattattttg aaacccaaaa agaaaattta
      601 tctaggtctc ctcttttcct tgatgattgg aatttttagt ataggaatta caaataccta
      661 tgcttcagaa aattctaatt tcaatatcaa ctcccaacca attgaagaac aagcaattaa
      721 ttttgcgaaa actgattttg aacactccat aacttcaatt cttttaatgg aaaataacac
      781 cgaaactgat attaactata ctcttggttc gccatttaaa atgctcgatg gagaaaatac
      841 ggcaaatagt tctattttct ttccagtatt ttcagacgct gataatacga ttaaatatat
      901 ttataccgtc aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc
      961 aaaagaactt caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga
     1021 taatagcatt tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag
     1081 tacagaaaaa aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat
     1141 aaaaaataaa attaaaaatt tctctaaaaa acaaaatttt cctgcgatta aacgtgtaaa
     1201 aagtgtctcc tccggagata actttattat gattaattgg cgtatagcag aaacccaaac
     1261 aagttttccg tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt
     1321 acatgctact tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca
     1381 aattgaatca aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt
     1441 ttatcctaaa aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa
     1501 aggaaatgcc atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc
     1561 acttgcatta tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc
     1621 ttggtataac tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc
     1681 tggaggtggt tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta
     1741 gttatgggtc tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa
     1801 aagactgatc tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta
     1861 gtaatcacta atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag
     1921 aacaatcata aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt
     1981 tacaaagcaa caaataattt actaacactg cgctctaata aaaaacaatt agattacact
     2041 tttagtttgg attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa
     2101 gggacagcta aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac
     2161 aaaacaataa tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt
     2221 gctagccttt tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg
     2281 cccctttgaa aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag
     2341 cttgccaaac ccttttagtg agctttactc attgtacctt tttaggtaca atggtattgc
     2401 gaacgatacg tccgtaggac gtactgaaag ggtgggactt atgagcgaac aaaatcaaaa
     2461 tttggctagc gatcattcta aaaaatatac ctatcgatcc gagccaaaac aaatcagttt
     2521 tcgagtgagc gaatctgaat ttgcaaagct gaagcagtca gctgaagctt tgcaaatgag
     2581 tgtgccggct ttcgtaaaag ccaaggcaca aaacgcgcgt ctggtgacac cgaaagtggc
     2641 gccagacatt gcccaggcaa tcgcgcgcga tttagccaaa gctggaggga atatcaatca
     2701 aattgctaag tggtgtaata cacaccaaca cgacgttgct cctggagacg cccaacgctt
     2761 atccgaaaac ctaaaaatca tgcaaaagga gctacaaaag atatggcaac agttaaagta
     2821 agccgaacca cttcttgtag tcgtgcaatt aattacgctg aaccacgagc aaccattaaa
     2881 acaggaataa actgtgacgt cgattatgcc aaaagtgaaa tgaaacaaat tcgcatgctg
     2941 tatggaaaag acgaccgtgt acaggcacac ttattgattc aatcttttcg accaggagaa
     3001 attaccgctg aaaaagctaa tcaactaggg gcagagtatg caaaaaaaat agcccccgat
     3061 caccaaatag ctatttacac acatacagac aaagaccata ttcataatca cattgtcatt
     3121 aactccgtta atctggagac tggaaaaaaa tttcaagctc atggtcaagc atttctaaac
     3181 aaatgttatg acctaaatga cgagatttgt cttgcacata acttaccaat cactgagcga
     3241 ggaaagaaac cagaaaaacg aaccatgtct gaaataaaat taaaagaaaa aaatgaacct
     3301 gtttggaaag acgaaattcg gattgcgatc gatcaaacaa tgaaaaacaa aaaaaccaga
     3361 acctatgacc aattttgtaa ttccctcaaa acattgggga ttcattgctt caatcgcggg
     3421 aaaaatttca cttacgaatt gatccacaaa aagaaaaaag tacgatccaa cagactagga
     3481 aaggactacg aaaaggagac gattttacat gagctggaca gacgagaacg aacctataac
     3541 caacagcgaa ccaacgagct acgaagagct cgagctagca ttcgcgaaac tgcagaagaa
     3601 gtacgaagag aacaacgaac tcttacgcct gaacaaccgg aaattagacc ggctcgaaga
     3661 aaccatgaac aacacgttga aagaggaaca gaccaccaac aagaattatc gctctaaaac
     3721 gttaaaagac atagaaaacg aaataaagaa acaacgtgct tttctcgttc cttatacgca
     3781 agaaaggata caaactacga ttgatttaca agttaaaaaa ctccatcaag agctattaga
     3841 ccagcgaaat aaggaacaga aaaaggtgct tattcaacga tcattggatt ttcttgcccc
     3901 cttatgttgc ttagtcctga cactgttcgc attttggtta gtatggggag gctaaaatgg
     3961 cattttattt accctatcta ctcatttttg ttagtatttc aggaagtatt tggctcatct
     4021 ataaaatttt tcaaacaaga tattcattaa aaggctctaa aattcgattt aagcgatttt
     4081 tctttcttgg ctgtatattt tccttgataa tcgtttcaag cggcttattc ggcgttttag
     4141 agggaaataa gcgcgtttct agaagtattt tattaggaaa tgtcactcaa aagtatgaat
     4201 cagcgcgtga taaaaagaaa aaagagcaag tattagcaca aaaaatagaa aaatttaccg
     4261 cttgttatga ggacatgaat gatatttttg tgaagcaaga aaagcgtttg acggataaaa
     4321 atatggaaac actcactcga ctttatcgga atttgccaga agaacagcaa aaagagtacc
     4381 aagaaaaata tgaacaagta aaaaaagacg tgcaatatgt gaaagatacg aaagtggaag
     4441 aagcttgtag tgatttattt ggagatacaa ttcctttttc aactagcgaa caagaacgaa
     4501 aagaacgaca acaatcagtc acatatgaac gctatgaaat cttgcttcaa caagcaatta
     4561 acatacaaaa tctgactaaa aaagaaacag ctttaaatta tttaaaaagc gtcaaggaat
     4621 ggttagatca gcaacagcaa aattgaattt gattgaaatt catgttaaac taaacatgca
     4681 aatgttcaaa cattagatac acgaaagccc cagagaggtc ggattctctg gggctttttt
     4741 taggttgatg tcgcctttac tgattttact tcttgtttcg gttattcaac caataactga
     4801 atacagagat acaaactcct acaagaaggg ggcaaataaa cagttcaaac atcagataca
     4861 cctccttcca gtcgcaagac tgtcggatag gcgacaagtt attataccct acaaaattaa
     4921 atctgtgtgg caattttaag tatctcttat ttccgggttg ttagggcttg ccctgaccgc
     4981 ctgtagggcg ctttaaatgc cagataaagg tttttatact aagactgtct taaaacggct
     5041 tagacaagct cttagagcct caaccctcct ttttgaacaa tggttttagc aaaggagctg
     5101 gaggcgactg cgcaggtata atgtgagagc gtagcgatca cacctttttg gcgaccgtag
     5161 ggagcgaggt tttgaattag cagtttagaa aagttcgcga acctgtttaa agaaaaaata
     5221 attgtttttt taggtgggaa attttgtctc cctcttttat aatctttttt taaacctctt
     5281 tttaaacctc tttaccgtcg gctgtgtcct tactccccca agggatacaa aattttatag
     5341 gcatgaaatt atctgtttat aggcatgaaa ttatctgttt ataggcatga aattatctgt
     5401 ttataggcat gaaattatct gttttaggta ttgtttttag acataatatg tcctaaaatg
     5461 atgtcaataa aatcatttag actggagtaa caactaaatg gcaaatgaaa tcgtaaagta
     5521 tgaaccagaa ttaaatacca ttcctttaag aaaatttacc ccaatagaaa tgaacttgtt
     5581 tttttctatt gtctctagaa tgcgcgataa aggtaatcaa aaaatacgct tttatttcag
     5641 ccaacttaaa gatttaagtg cgtataagca gactgcagga gaagtcttta taaaagattt
     5701 ggcaagaaca tacgatcatt tgatggattt acgtttcgga agaaaaagca atggtggatt
     5761 aacaatagaa cgatttacaa tgtttactga ttttgaaatt catggagaag tcgataaccc
     5821 ttatgtagat ataaaagtcc acgaaaaagc tttgccttta cttaataatc tagaaagctg
     5881 ggtacgtttc tctttaacag aatttcgaga tttaaatagc agttatgcta aaacaatgtt
     5941 tagattgcta aaacaatacc gaactcaagg atgggctgaa ttttctaagg aagatttttt
     6001 agagctatta gatattccta aaagctatag acaaacaaat ataaatcagt ttgtactcaa
     6061 accaataaaa gaagaactaa ctccactatt taaaggatta actattcgta aaaaatatgg
     6121 taaaggtcga ggtaaacctg ttattggtta tcgttttact tggaaagctg aaatcaacca
     6181 tgctgacgat ttttcaaaag ggaagcaaga agatttacgt ataaaattat ttaatattga
     6241 acacaatgga gaattaacac aaaaagaaaa atggcgtgct aaagatcgaa tactaaatct
     6301 tccgttaggt actcatgaag ctgattttaa aaaacaacaa caaaccgaaa aagaagaagc
     6361 ggaaaaacaa tcaatctcaa atgagcttaa acaagattta ttagaaaact tacaaaactt
     6421 attcgattga agcataagga gtatttatga tagataaaac gatcaaagaa ctcgcggaag
     6481 aatttcatgt ttcaaaacaa gcaattagga aaaggctatc agctgatttt agagaaaact
     6541 atgtgcaaac tgttactagg aacggagtga aaaccttagt tgtcagctac aaaggatatt
     6601 tgcttttaaa acaacatttt ttaggtagta acaccactgg taaccaagta gaaaccgttg
     6661 ctagtaacga tgaaaactcg attattgttt ttttacatga acagctccaa gaaaaagata
     6721 ctcaaataaa acaaatgcaa aagttactcg accagcaaca acaactcacc ttgcaggcaa
     6781 atcaacagat cgaaaaactg caagaacagt tacaattagc ttatacggaa gaacctgaag
     6841 aagaggaatt aacttctgtc aataaagaaa acaacaatgc aataacaaca gaaaaaaagt
     6901 ggtggaaatt ttggtaacaa ttaccaactt tccataaaga agaaatcaat ttctatagac
     6961 tctatctttc taagaataaa aaatatgttt aggtgatcaa atgactataa gtaaagtaat
     7021 caaacaaaaa agaatagaaa aacaactaac tcaagaagat ttagctgaaa tgcttttagt
     7081 atctaaaaaa accatttcta attgggaaaa tggtcgaaca ataccagata cagaaaattt
     7141 aattcagttt gctaagttat ttgatttatc actagacaat attttgttag gagaagaaaa
     7201 aattatggta aaaaatcata caaaaacact aatactatta tttcattttg taattttttt
     7261 aagtttagtt ttcaccttaa ttcagtccgg attcttttat tttagccatt caattgccac
     7321 ttggttatta atattttttc taagtttact attaataatt aatactttaa ttattatctt
     7381 taataaacat agaaacttat ttcttcacag catatttcca attgttttat taatttttct
     7441 atactttata taaaaagata gaactattta gtaagataaa aaagtagcta attgtaattt
     7501 tcgtctaaaa ctcaaaacta gtaaaaataa ctttattgtg ttatttttat ttataaatta
     7561 tgaaaaagga cctttcgaaa tgaaaaataa tagaatctac aaaaatcagt tgaattatgg
     7621 gcttttattg ccagtttttc tgctatgtat cattggatta ctttctttat atgttgcttt
     7681 atatcacgac tcttacacta attcaaaaat cacaaaagaa ttaattaaac aagtattgtg
     7741 gtatagtatt gggttcactt cagcaataac tatttatttt tttaatccta aaattttgtg
     7801 gaaattaact cctatatttt acggattatg catcataatg ttattacttc tcttgaaatt
     7861 ctataatcca caattagcag tcttaactgg agcaaaaaat tggttttata ttgctggttt
     7921 ttcgtttcaa cctactgagt tagtcaaaat aggatacacc cttatgctag ctaaaataac
     7981 cgtcaattat cagcaaaaat ctattactag taatacgaaa gatgattgga tttatttact
     8041 taaaactttt attgctactc ttccaatagg aatattatta ttttttcaga acgatctagg
     8101 aacaatgtta gtcttcattg ttatttatat ttttattttg tttacgtcta acataaatat
     8161 aaaaataatt gccccaataa tcgccattgg tgttgtttta tccttaatat ttttatattt
     8221 ggttatatca gatacaggaa gagatttctt attaaaaata ggatttcacg actatcagtt
     8281 taaaagaata gactcatggt tgaatccatt tttagatcca aatggatcta gctatcagtt
     8341 agcaaatagt ttaattgcta ttggctctgg cggactttta ggtactggtt ttaatgtatc
     8401 aaacattcac gttcctgtta gagaatccga tatgatattt tctgtaattg gagaaaattt
     8461 tggctttata ggaagttgtc ttgtaatatt cctatacttt tatttaattt atcaaatgta
     8521 taaaacatgt tttaaaagca ataacttatt tttcacctat atcggtatag gcattgttgc
     8581 aatgattttt tttcatgttt ttgaaaatat aggtgcaagt atcggattac tacctctaac
     8641 tggtattcca ctcccctata ttagtcaagg tggttctgcc attcttagta attttttggg
     8701 gttgggattt atgttgtgtt gtagtagata tactaacgaa aaaaaaggaa cacaaaaatg
     8761 aattcaaaat aacactttat gaatagaatg tctatttcaa taaaaattat gataccgtga
     8821 actactcacc acttagctaa acctgacggt tcttaacgct tgaagtggga gcttcttggg
     8881 aatagagcat acttgatagc gtatttcttt actaacagcg gtgtctctta tttaccaagc
     8941 tatccccgta gttcctacgg ttcttgatga ttggatctaa gccaatccca tagaatgtag
     9001 tcttagacct tcggtcagaa tattgatact agcattgata tcacgatcat gatgagtatg
     9061 acaaacagga caagtccatt ctcgaatttc aagagatttc ttgccatctt tatgtccgca
     9121 ctctgaacaa atctgactag acggaaacca tttatctact ttgatgattt ttcgtccata
     9181 ccagtcagcc ttgtattgta atttagtcac aaactttgac caagaaacat cagaaatact
     9241 tttagctaat ttatgatttc gcaacatacc ttttgtgttt aagtcttcaa tacagataat
     9301 atcgtgattt ttgatcattt ctgtactcaa cttattcaga aaatcagtac gttgattcat
     9361 tactttttca tgcaatctag ctactttgcg tttttgtttt tgataatttt tagcttcaaa
     9421 tagattggta cctttatttt tagccaataa agcacgccta gacaatttgc gttgttcacg
     9481 ctttagtttc ttttccattt tggacgtgaa tttattatta tcaatttttt gtccatcaga
     9541 aagaatcgca aagtccataa tacctaagtc aataccaatt gtggaattag ttttaggtaa
     9601 ttcgataatt tcttctttac acaacaaaga aatatagtat ttaccgct
//
LOCUS       DAJSKS010000108         9458 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJSKS010000108 DAJSKS010000000
VERSION     DAJSKS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.110"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            67..402
                     /locus_tag="ON396_002149"
                     /pseudo
     CDS             67..402
                     /locus_tag="ON396_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            567..794
                     /locus_tag="ON396_002150"
                     /pseudo
     CDS             567..794
                     /locus_tag="ON396_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            920..1891
                     /locus_tag="ON396_002151"
     CDS             920..1891
                     /locus_tag="ON396_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3114218.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            2031..3344
                     /locus_tag="ON396_002152"
     CDS             2031..3344
                     /locus_tag="ON396_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR3114219.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            3387..4253
                     /locus_tag="ON396_002153"
     CDS             3387..4253
                     /locus_tag="ON396_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3114220.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            4250..5098
                     /locus_tag="ON396_002154"
     CDS             4250..5098
                     /locus_tag="ON396_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3114221.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            5112..6584
                     /locus_tag="ON396_002155"
     CDS             5112..6584
                     /locus_tag="ON396_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCR3114222.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            6601..7473
                     /locus_tag="ON396_002156"
     CDS             6601..7473
                     /locus_tag="ON396_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3114223.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(7708..9090)
                     /locus_tag="ON396_002157"
                     /pseudo
     CDS             complement(7708..9090)
                     /locus_tag="ON396_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2875 a         1643 c         1972 g         2968 t
ORIGIN      
        1 ttttgaatga atgaaattaa gcgcaataaa aattcttttg gtgtttattt aggagagcct
       61 agctcggaac agttaaataa aggtgaagct cgtcatgctc tatgtcgaaa tatattttat
      121 ggtagaaaag gcaaacttta tcaaacttat tacgacggta tggaggaaca gctcaacgca
      181 ttaggattag taactaatgc tatcatatat tggaattcac tatatttaga aaaagtcttt
      241 gaacaaatta aaacggaagg gttcgactgt tccgaagaag tgttacgcaa gcaatttcca
      301 ttaattagtg atcacattaa ttttgttggc aaatatagtt tccgctatga aaaagaatta
      361 tcgaatggaa tactacgtcc gttaaatata gagaattatt gagaatgttc gtcttttggt
      421 tcctaccgtg cattttcgca cggatgttcc agatactatt tttgaatgta taaaattgaa
      481 aacacaagaa tactaataaa tcaacgattc ttgtgttttt aatttttcac taccgtgcat
      541 tttcgtttct atgttccatg taccccaatc ttgaaaaatt aattaaagac ttagataaag
      601 ccattgacga cttagtcgtg gtgctaccgg agtatcaatg tttaaccagt atcccaggta
      661 ttggtcctgt ttaggctgct ggaatactcg ctgaaattgg tccaattgaa cgtttttctg
      721 atgaaataaa attgacaaag tatgccggcc tatattggcg acagagtcaa tccggtaaat
      781 ctgaatacga aaattttttt acgaaaagct tcacgtctaa tatctatttt aaaataaaaa
      841 tattcctgct aatttggaat cgtttccttc ttttggtata ataatacctg aaattctttt
      901 gagaggagtt gttcgtgaag tgaaaactac tatgaaggat gttgctgcgt tagcaggagt
      961 tggtgtgggt acggtatcta gagtaataaa tggagtaaag gtgaaagagg ttaccaaaga
     1021 aaaagtaatg gctgcaatta gtgagcttaa ttatgagcct gatgaatatg ccagaggtat
     1081 gaaaacgaat agtagcaata caatagctct tattttacct acgatttggc atcccttctt
     1141 ttccgagttt gcctactatg tagaggaagc tctcagccaa aaaaattata agcttttttt
     1201 gtgtaatgcg aacggcagtg cggaaaaaga aaaagaatat atccaaatgg tcaagcagag
     1261 taaagtagac ggaattatag gtattaccta ttcagacatt gataaatatg tatcatcaaa
     1321 tttaccattt gtcagtatag accgccattt ttcggaacag gtttcttatg taacggcaga
     1381 taattattta ggtgggcaaa ttgccgctca agaactactt actcgtaact gtaaaaactt
     1441 gtgctatgtt gggggtgttt caccttaccc taacgaaaca aacaatcgta aagctggttt
     1501 ttacaatatt tgtgaggaaa ataaaatagc tgtgaaggta ttggatatgc ctgaaccaat
     1561 tagcaattta gaggaacaat tgctcgcttt tttttcggat aatcctgaaa ttgatagcat
     1621 ttttaccatc aatgatttca tggctttgga tgtgataaaa gcccaaaaaa aactagggaa
     1681 ggaagcacct aaggattttc aaattattgg ttttgatgga gtcaagctat cgttgcaaaa
     1741 agactattta ctatcaacga ttgtacagcc gatagaggaa atggccaagg tttcagtaga
     1801 aacactcatg gcgatgattt taaatgaaga accgcctaag cgtgtggtag taccggtttt
     1861 ctttcacgct ggagagacga ctaaagagta aaaaaccggt ttataacgat ttttttgaga
     1921 taggttgaca gaaagtgatt tcaatgatat cttaaaacca attaattgga aacgttacca
     1981 attaattgaa aaataaaacg gaaacgtttc aaaaaatagg aggaacatcg atgaaaaaga
     2041 gaatgttagc aattgcagca attttagcat taacagctgg aggacttgca ggatgtggca
     2101 gtaaggagaa aagttcaggt agcagtgatc caggagaaat taagatttgg gttcagtttt
     2161 ctgatgaaac gcctgaagga aaagcttggg cagagattgt gaacaatttt aatgatgaat
     2221 acgagggtga atataaagct gttactgagt atattcctcg tagcggaagc ggcggtggat
     2281 atgaggataa agtaaatgct gcaattacaa ccaataccct tcctgatgtt attacgttag
     2341 atggaccgaa tactgcagct tatgctcatt caggagtcat tgctccatta gatgattatt
     2401 tgaaagatgc ggacatggat gatgtattag acagcattat tcagcaagga acctacgatg
     2461 gcaaaatgta tgcgtttggt ttctcagagt ccagtgtggg tgtctattac aataagtcgc
     2521 tgttcaaaga tgctggaatt acagaagatc aattgccgac gttagaagat ccttggactt
     2581 ggacggaatt tcaagcaatt tgccaaaagc tgactgataa atacaaaacg cctgcaattg
     2641 atatgcaaat ggcgtcggga gacgaaatgc tgacctacgc atatacacca tttatatggt
     2701 cgaatggcgg cgatgtcgtt aatgctgatg gaaccaaagc cgaagactat tttaacggta
     2761 aaaaatccgt tgaagcgttg gaatttattc aagaattggt tgaaaaaggt tacaccacaa
     2821 tcagtcctat tgaaaaagga tttgaaactg gcaaatatgc catgatgctg agcggttctt
     2881 ggacaatggc agatttagag gaaaactatc aagagattga ttatggtatt ttgccttacc
     2941 ccgtttcaga tgcaactggg gagttagttt caccaaccgg aagctggcag ttagcaatga
     3001 ctgagaaaac agagaaaaag gatgcttctg cggcctttat taagtttgca accagtacag
     3061 aatctagtcg tattatgagt ttagggaaca gtgttctacc tatcagaaat tcaacagttg
     3121 atttaattaa agatgaagtg tcggaaggaa tgcaggtatt aattaagcaa aaccaacttt
     3181 ctggacatgc ccgcccagtg gttgtagctt atccgcaagt atctagagca ttccaacaag
     3241 cagttcaaga tattagctac ttcgaggaaa acccagatgt acaaaaagta gcagatagtc
     3301 gcgctgctga aatgcaacag gcaattgata cttcactaaa atagaagttt ctaaaggggg
     3361 ccaatcaagt gttaaaaaaa ccgacaatga aagacaattt gataggttac ggctttctag
     3421 cgccagcttt gctgctgctg acgatttttc tgatcatacc cgtaatcatg gtaatttatt
     3481 atgcctttac tgattattat ttgttaacgc cagatgctag acaatttatc ggcttagaaa
     3541 attttaagcg actgttcgct gatccaattt ttgtaaaaag tatttggaac acggctaaat
     3601 ttgttgtttg gattattccg gtccaaatag gggcagcttt aggaatggca cttattatca
     3661 acaaaaatcg taaaggaaat atctttttta aagtagcctt ttttgcgcca gtcgtgatgt
     3721 ccttagtcgt tatttcgatt ttatggttgt acttgttaaa tcctaatgaa ggtctggtta
     3781 atacgctatt tgcaaagatt ggaattgctt cgcagccttt tttgactagt cccaaacaag
     3841 caatgtatac gcttgttttc gtatcggcat ggcaaggtgc aggatatcag atgctgctgt
     3901 ttttaggggg aatgcaaaat attccgcaag acgtttatga ggcagccgaa attgatggtt
     3961 tttctaagtt tcagcaattt cgctatatca ccatgccatt attaaagccc acggcgattt
     4021 ttattttact taccacattg atttcggcct ttaaactaat cattcagcca atggtaatga
     4081 ctcaaggtgg accaatgaat tctactatga caatggttta ttacatttac caaactggat
     4141 ttacagaccg tatggtaggc tactccagct ccattgcgtt aatctttacc actatgattg
     4201 gtttaattac aattgctcaa agaaaattgg taaaggagga tgatccagca tgaccaagaa
     4261 atttggaccg ctaatgatcg tagaatattg tttgctgttc cttttagcgg cattattcat
     4321 tttcccgatg ctgtggatgg tagtttcctc tatgaagcct gaagcagatg tctatcaaaa
     4381 tttagcttcc ttcaaagctt ttttacctag tttgaatcca gctaattggt ttaaaactta
     4441 tcaagaggtt atttcccggt tcagtgtggg aacttattta ttaaacagta ttttttatgg
     4501 tttaaccttt gcctgtgggt caatcattgt aaactctttg gcgggttttg cctttgcgaa
     4561 ggttaatttt tcaggcaaga aaattatctt tggagctttg ttagctctgc tgattgtccc
     4621 gatggagacg gttttaattc cgcaatttac catcgttaat tcactaggct tagtgaatac
     4681 tcgcttggca gtgattttac cagcgatggc cagcgcgttt aatatctatc tgtttagaaa
     4741 tttctttatc gcgattccag aagaaatcat tgaatcagcg aaattagatg gtgcaaatat
     4801 tgttaaaatt ttctttcaca tcatgctgcc aatgtctaaa ccagccatag ccacagtagg
     4861 tgtcttatcc tttatctcca gctggaatga ttatatttgg ccgttaatgg tcttaactga
     4921 cagcagcaaa ttttcaatgc aagtggcaat tactacgatc aatactacac agccagttta
     4981 tatcaatcaa gtgatggctg ttttaaccat tgcaacgatt ccactgatta ttgtttatat
     5041 cgttgcacaa aaatatattc tgcagggact tggcggttct ggaacgggta taaaatagga
     5101 gtgaatagtg aatgttgcaa aaacttttaa aggccaatga gtttattgag aaaaatgctc
     5161 aaacagtcaa tcaagaatac cggaataaat ttcatttgat ggctccgatt ggctggatga
     5221 atgatccaaa tggttttgtt tattatcaag aggagtatca cttgttttat caatattatc
     5281 cttacgacag catctgggga ccaatgcatt ggggccatgc taaaagcaag gatctaattc
     5341 actgggagca tttgccagta gcactggcac caggtgaaag ctatgacaag gaaggctgtt
     5401 tttccggcag tgccatagaa aaggatggca agctgtattt gatttataca ggacacaatg
     5461 ttgtggaagg gcaagttcga caagttcagt gtttggcagt ttcagaagat ggcattcact
     5521 ttgaaaaata tgccggaaat ccaatcatcg cagaagatca cttagcttct gttgcaacga
     5581 ccgaagattt ccgcgatccc aaagtgtttc agcgaaaaga agactatttt actgtagtcg
     5641 ctactaaaac tgcagatgct cgtggacaaa ttgtgatgtt ccattccaaa gatttactaa
     5701 cttgggagtt ttattccgta ttgctggagg gaactccaga acaaggcatt gtatgggaat
     5761 gcccagactt gtttgaacta gatggcaaag aagtcttgat tctttctcct attcaaatga
     5821 ctccacaagg caatgcttac caaaatacga actcaactgt tgctttcatc ggtactgtcg
     5881 attggcaaac aggcaaattt ctagtagaga attttcatga aatcgatggc ggtttagatt
     5941 tttatgcacc ccagacctgt gaaaacgaag caggccaaag aataatgatt gcttggatgc
     6001 aaatgtggca gcgtaaaata cctacgaatg atctgggcca tggttggtca ggttccatga
     6061 ccttgcctag agagctgagg gtggctgata atcaattgtt gcaacggcca gtttcatcaa
     6121 tttatcaagt aattgatccg caggaaagga ttgaatgggc gaagactgaa gagaatcgct
     6181 tcttactgaa ggacttagta tatgaccagc actatttacg cttggaaatc aatttaaaac
     6241 aggcaacgaa actagaactt cactatgcga agggacccga ccatacatta ctgcttaact
     6301 atgatgttga acgggagtgc tttactgctt ccagggaaca catgccgtat gatttatcag
     6361 gagctgaaaa ggaagtgttg aaaaagcgca gtgtgacgat tcctttggaa aataattgtc
     6421 taaccatcga aattttccgt gatacttcag ctattgagct ttttctaaat gatcgtctga
     6481 ctatgagtac aactttttat gagacagaaa aaggacagga ttttcttttg ctggcagaca
     6541 gtgaagtaac cttttcattg gaaagcggct taattaatct atagaaggga gagctcagtg
     6601 atgatttatg gttcaatcga agcaggcggc acaaaatttg tgtgtgccat tagcgatgaa
     6661 gaaatgacga tcaaggaaag agtgagcttt ccgacaacga cacctgagga aacaatacct
     6721 ttagtcattg acttcttcaa acaataccaa gcagatttag cagggattgg gattggctca
     6781 tttgggccta tcgatatcca tcgcgattcc gccacttatg ggtacattac ctcgaccccg
     6841 aaagtggcat ggcaaaattt tgatttcatc ggtaccatga aaaaagaatt tccgatcccg
     6901 atttcttgga caacagatgt caatgcggct gcctatggtg agtatgtctt cggcagtggc
     6961 aaaggattga gcagtgtcgt ttactatacg atcggtacgg gtgttggcgg cggtgcctta
     7021 caagaagggc gctttatcga agggtttagt catccagaaa tggggcatat gctcgtcgtc
     7081 cctcatccaa aagatgactt tgctggcagc tgtccatttc atggcaactg tttagaaggg
     7141 atggccgctg gacccgccat cgaaaaacgc ttagggaaaa aaggacaaga ggttgcagaa
     7201 gatgatcctt attgggaaat cgaagcctcc tatattgctc aatgtgccta caatacaacc
     7261 ttgatgtttt ctccagatgt gatcattttt ggcggtggtg tgatgaaaca acgccatatg
     7321 ctgcaaaacg tccacgacgc atttgcaaaa ttggtcaatg ggtatgtcga aacacctgag
     7381 ttaagcaaat atatcgtgac cccagcatta gaagacaacg ctggaacgct cggttgtttg
     7441 gcattggctc gagaagcggt cttacattca taaacgaaaa aaggctcttc gacatttagt
     7501 gttggtagag aaagatacaa ctgaaaattt agtcataacc agttaggcag agtgcttaac
     7561 tggtttttgt tttacttgga agatcaagcc ttgaatgaga taagttcttg ctcgaaagtt
     7621 atggaaattt cgatagccgt aggctacttg tattgtgcaa tagtttttta caaaaaaagg
     7681 gggtgcggat attttttttg gaccttttta acaaatatat ctttgcttct tccgatattg
     7741 aagagggctt agccttccta aagataactt aattcgttga ttattatacc aatgaatata
     7801 atcatccaat ttgtttataa actgattgat tgttgtattc ttccagtcac gttgataaaa
     7861 catctcgttt tttaatcgtc caaagaaacc ttcgcacgct gaattatctg gtgaataact
     7921 ctttttagac atagagtgaa gcaagttggc tttgttccaa tagtccaact aacaatagca
     7981 ccatcgtaac aatcgacaag tggtgataag tagattttcc ctgcgagaat tctaaattca
     8041 gtaatatctg ttaaccattt ttcatttggc ttctttgctt taaaattccg atttaataaa
     8101 ttggggtgtg caggagaaat ttccccagca tacgaagaat acttttttcg tctagaaaat
     8161 ttaacaaata gattttcctc agtcatcaat ctacgtacaa ctttttcaga aagcttttta
     8221 cccatatttt ttaaagcttg gtgaattcta cggtaaccat acgcacagaa gttatgattg
     8281 aaaatcgtga taattaattt gacgttcttt gtaatactta tctggtttct caagtgcttg
     8341 tttttgataa aaatagctgc ttttaggaaa ctggagctgt tgaagaagct ctttcaaggt
     8401 gaactgattt cgtaaggcat caatcaatat cgttttttct tgatttgata gacaaagaag
     8461 attgacgcct ttttcttttt ttagcagttc agccgctcct tcgagtaatg ctttttcgag
     8521 ctgtaattgg tgtacttgtt tttgtaagtc ttctacttcc tttaaaagta gctctttttg
     8581 tttttcctga ttttttaaag tcatacacaa tggaaaatcc tttccaagaa ccgtgttttt
     8641 ccattgatat agagtaactc tactaattcc tccagattcg gctaaatttt tcctattagt
     8701 ttgcggttta tagaattttt tcaacacagc ttctttttga tcttgtgtta actttaccgc
     8761 actttttcgg agttttctag ctcctggtgc aagttcttca caccgctgcc gcaaggactc
     8821 tctattagga taaccaagca tgcgaatggt tcgtgcataa cattgaccat attcaaaata
     8881 atgattcacc gctattcgtt tttgttcttc agaatacttt gatttttttc ggtgctcctg
     8941 atggagctct ccggattcca aatactcttt ataccatttc cgtagtgcac caatcgacgg
     9001 ataccctaat tctcgaatgg ttgcagcata agagcaatcg tattttaaaa ataactgaat
     9061 tgcccttaca cgctctttgt gggaaaacac acggacattc ctcctaagtt tattttttcg
     9121 tccaggatta tgtccgcacc cccttgcttt ctgatgactt attctggttc tttgagtgtc
     9181 aatgcataga tgtgatgtag ctagttagaa aaaaattttg aatactcttt ttttctttca
     9241 gactcctttt taagtaagat tttgaagtgt gagttacggg gtcagaaact taaaagtttt
     9301 tgatccttta ttttcaagtc tgatttggca gcttttcggc agctcattta tttatggtca
     9361 gaaatagatg ctgaaatttt aatctattag caaatagtcc agaacgtatg aagaggagtc
     9421 ggatatcatt tgtcattaac accataaata tttataaa
//
LOCUS       DAJSKS010000109         9252 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJSKS010000109 DAJSKS010000000
VERSION     DAJSKS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.111"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..651)
                     /locus_tag="ON396_002158"
     CDS             complement(<1..651)
                     /locus_tag="ON396_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3114224.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPL"
     gene            785..1072
                     /locus_tag="ON396_002159"
     CDS             785..1072
                     /locus_tag="ON396_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114225.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1488..3026
                     /locus_tag="ON396_002160"
     CDS             1488..3026
                     /locus_tag="ON396_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCR3114226.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3023..3988
                     /locus_tag="ON396_002161"
     CDS             3023..3988
                     /locus_tag="ON396_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3114227.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3975..5297
                     /locus_tag="ON396_002162"
     CDS             3975..5297
                     /locus_tag="ON396_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR3114228.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5360..5464
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5538..6281
                     /locus_tag="ON396_002163"
     CDS             5538..6281
                     /locus_tag="ON396_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3114229.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6296..7288
                     /locus_tag="ON396_002164"
     CDS             6296..7288
                     /locus_tag="ON396_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3114230.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7300..8037
                     /locus_tag="ON396_002165"
     CDS             7300..8037
                     /locus_tag="ON396_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3114231.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8027..8749
                     /locus_tag="ON396_002166"
     CDS             8027..8749
                     /locus_tag="ON396_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCR3114232.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8833..>9252)
                     /locus_tag="ON396_002167"
     CDS             complement(8833..>9252)
                     /locus_tag="ON396_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCR3114233.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3026 a         1598 c         1958 g         2670 t
ORIGIN      
        1 aagaggatca agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac
       61 agatccaatt tccagatact tcactttatt caatgtataa gatagaatag aacagctaag
      121 taatgtatac agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa
      181 agtaatcaag aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata
      241 aatgatatct gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc
      301 agaaagaata cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg
      361 aaggctctcc cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag
      421 aaaagcgtgt tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag
      481 tgaagtgaca gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac
      541 cccaccatct gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc
      601 tgcgcctaaa atgattccgc acaattctgt aaataaattt aattttttca tttctagcct
      661 cttttctttt gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa
      721 gaggaaaatg aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg
      781 aaaaatgcga gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta
      841 tcaaggagtt tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa
      901 taaagcaaat caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga
      961 catctttctt tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga
     1021 actcattcct ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat
     1081 aagaaaacaa gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt
     1141 gtcatgatac agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat
     1201 ttctacttta ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat
     1261 agctgattcc aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag
     1321 aaccaaaaag ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg
     1381 tatttttttc ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa
     1441 aaggaaagaa atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta
     1501 tacaaacatt aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca
     1561 tttctctcat ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag
     1621 ccaatcataa aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc
     1681 cttcacttgc cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat
     1741 tgattgcact tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag
     1801 ctgaaattga cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac
     1861 gcttgctgaa agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg
     1921 ccttagtttt aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag
     1981 ggacatttat tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga
     2041 tcagttctgc cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga
     2101 aacctcgata ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt
     2161 tatatcagtc tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg
     2221 aacctgaaat cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag
     2281 ttgaattaaa accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc
     2341 aaatcgatat ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg
     2401 aagcaatgaa aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat
     2461 tgaataagca attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct
     2521 tggcagtcaa gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag
     2581 ctatctctga tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat
     2641 ataaaggaat ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa
     2701 gtttgcgtta ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag
     2761 atagccagct caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag
     2821 cttatcaagg cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta
     2881 aagcactgaa taaattaagc ggaaaaataa cagaggatca aatgattgat atgaattacc
     2941 aagtcaatgt cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag
     3001 gattagtaaa ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa
     3061 gtatgatgga aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt
     3121 tgttttggtt ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt
     3181 atctaaacca tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct
     3241 gcagaaaatg cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat
     3301 gacagtcaag caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag
     3361 agcagaccgt gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga
     3421 tcgtatgcct agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat
     3481 tgctgcaagc ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc
     3541 aagaaactca ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt
     3601 tttcgttacg cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa
     3661 ggatggagaa attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga
     3721 ctacgttcgg catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt
     3781 agtcgtcaat ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt
     3841 tgatacgcct atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt
     3901 agaaaagcaa agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg
     3961 agaggaggaa ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct
     4021 taagtgctgc ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc
     4081 tatggggcgg gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag
     4141 tagaggcacg taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg
     4201 ccaactggga agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc
     4261 gaaaaaaaca attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg
     4321 atgattcatc tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa
     4381 ccgggcaaag acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag
     4441 actttttatc attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg
     4501 cttttgagct agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata
     4561 atcaacggcc attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg
     4621 aagcatctgg cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta
     4681 ctcgctatcg tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga
     4741 ctgggagaca gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata
     4801 aacacggaat tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg
     4861 gagatattgt ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt
     4921 atgtagctaa gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta
     4981 cgatcgtcta cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt
     5041 cgtcagatca agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg
     5101 aaccagttgc taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag
     5161 tgattagcga gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat
     5221 tgactaaaaa agagttggat agctatatca tggggtatcc gacattggca agtgatctct
     5281 cctatttatt gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg
     5341 ctacacttta aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt
     5401 tgtgaactta gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga
     5461 cgcttgaagc gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga
     5521 agaaaggagg taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt
     5581 taattatttg gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac
     5641 ctgctgctgt attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca
     5701 cgacattaaa tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg
     5761 ccttggcaat ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt
     5821 cacaaatgtt gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta
     5881 tgcttccaaa agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt
     5941 cggatacatt gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag
     6001 atacagggaa gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg
     6061 gtttaaaagt ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg
     6121 ctcaagcagg cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag
     6181 tttttgctgc gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat
     6241 tattgaaaaa agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa
     6301 aaaattatgg cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga
     6361 aacaagtagc aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt
     6421 accgaatact aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc
     6481 tggtttaaat gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg
     6541 tgcggacaaa gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga
     6601 tggacagaat ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt
     6661 cgccacccta tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc
     6721 aggttggcaa agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg
     6781 ggacttttca aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa
     6841 atcctcagat atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg
     6901 tattgaacta aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc
     6961 ggtaattatt acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat
     7021 ggatgccacg aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact
     7081 gttgcaaaaa tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt
     7141 gtcaaaaaat tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg
     7201 caattataca gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga
     7261 actattcact aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac
     7321 ttgaatgtag tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa
     7381 gataccttta catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact
     7441 ttaacaggag tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact
     7501 ggtctccata cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc
     7561 gtctatcaaa acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa
     7621 gtaagggatt atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa
     7681 ttatctggcg ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct
     7741 tacttgcttc ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa
     7801 gactggatgt tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat
     7861 atcgaagaag caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat
     7921 gtgattgctg agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa
     7981 tcagagttga agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc
     8041 tcattgtcat ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt
     8101 gacgatcatc aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag
     8161 taagactcaa gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga
     8221 acaagttgag ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat
     8281 ttggacgaat gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc
     8341 agccataaat gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa
     8401 ttatatccaa gcttacccta atcactattt gatccattgg tattcttctt tggactatca
     8461 acaagactat attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc
     8521 agcagtctgg caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg
     8581 actacatgca gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact
     8641 gcttgaagaa cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa
     8701 aaaaaatact tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt
     8761 gacacaacta gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt
     8821 aataaatgaa tcttagtacc aaccgtttgc ttcccagaat gctttcgccg cttcccaaga
     8881 accataacgt gaagtaacat attgttctgc tactctttct tggtttgcag cagagtagtc
     8941 accatttaag taagaagaat ctaattggta acgtccgatg taacgtccgt tcgttgctgt
     9001 gtaagaaccg cttgattctt tttgtgcgat ccattctttt gcagagcttg tgcttgctgg
     9061 agtagcagca gtttctgtta cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg
     9121 tttttccgtt tgaaccgttt cttgtacaac aggttgttca gcttggactg gtgcttcttg
     9181 aacggctgct tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg
     9241 ttgtcctgag ta
//
LOCUS       DAJSKS010000110         8738 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJSKS010000110 DAJSKS010000000
VERSION     DAJSKS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.112"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..1947
                     /gene="topB"
                     /locus_tag="ON396_002168"
     CDS             <1..1947
                     /gene="topB"
                     /locus_tag="ON396_002168"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR3114234.1"
                     /translation="KATRKDGYFEIQDRLFTGETVITYGFGHLVELDSPDMYDEKWKQ
                     WALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADTIVIATDSDREGELIAWSIIQQ
                     AGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYPKFEEVQARQIADWLIGMNGSP
                     LYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRNFQKEPYFEGKAQVIAQNGAFD
                     AKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVETEKKSAASPRLFSLSSLQSKM
                     NQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEGEYAYLLDHLDEYKHFLKAEAI
                     PTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQLSPLQQAIYEQVLKTTVAMFA
                     EKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGKESDGKEKEEEPLLPKVTKGEM
                     VTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSEEAQSILKEVEGIGTEATRANI
                     IETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLTSAEMTAKWESYLLKIGERKGT
                     QTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKAASEKQEAARHLGICPKCQEQE
                     VLLYQKVAACTSEACDFKRSEGFI"
     gene            2063..2212
                     /locus_tag="ON396_002169"
     CDS             2063..2212
                     /locus_tag="ON396_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114235.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            2293..2889
                     /locus_tag="ON396_002170"
     CDS             2293..2889
                     /locus_tag="ON396_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCR3114236.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            2902..3801
                     /locus_tag="ON396_002171"
     CDS             2902..3801
                     /locus_tag="ON396_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCR3114237.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            3804..3935
                     /gene="ssb"
                     /locus_tag="ON396_002172"
     CDS             3804..3935
                     /gene="ssb"
                     /locus_tag="ON396_002172"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3114238.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            3957..4289
                     /gene="ssb"
                     /locus_tag="ON396_002173"
     CDS             3957..4289
                     /gene="ssb"
                     /locus_tag="ON396_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3114239.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            4311..4568
                     /locus_tag="ON396_002174"
     CDS             4311..4568
                     /locus_tag="ON396_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114240.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(4727..4849)
                     /locus_tag="ON396_002175"
     CDS             complement(4727..4849)
                     /locus_tag="ON396_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCR3114241.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            5039..5263
                     /locus_tag="ON396_002176"
     CDS             5039..5263
                     /locus_tag="ON396_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114242.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRNQLVIESMQIRNPDHFTRLMGI"
     gene            5711..5917
                     /locus_tag="ON396_002177"
     CDS             5711..5917
                     /locus_tag="ON396_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCR3114243.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPYTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            5917..6168
                     /locus_tag="ON396_002178"
     CDS             5917..6168
                     /locus_tag="ON396_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114244.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            6184..6621
                     /locus_tag="ON396_002179"
     CDS             6184..6621
                     /locus_tag="ON396_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114245.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGQWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYLENVNKYGACYIMKKEDNENE"
     gene            6614..7309
                     /locus_tag="ON396_002180"
     CDS             6614..7309
                     /locus_tag="ON396_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114246.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEELNSKDATVDDYYLVFTKFLTCGTE
                     SIVLMGMDYELAVPEVFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRK
                     RMPYQRFERKKGQ"
     gene            7690..7869
                     /locus_tag="ON396_002181"
                     /pseudo
     CDS             7690..7869
                     /locus_tag="ON396_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7931..8115
                     /locus_tag="ON396_002182"
                     /pseudo
     CDS             7931..8115
                     /locus_tag="ON396_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            8149..8580
                     /locus_tag="ON396_002183"
     CDS             8149..8580
                     /locus_tag="ON396_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR3114247.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT         3128 a         1500 c         1819 g         2291 t
ORIGIN      
        1 aaagctaccc gaaaagatgg gtattttgag atccaagatc gattgtttac aggagaaact
       61 gtgatcacgt atggttttgg tcatttagtc gagttagatt cgcccgatat gtacgatgaa
      121 aaatggaaac aatgggcatt agaacatttg cctattttcc ccaatcaata ccactatcat
      181 gttcctaaag ataagaaaaa gcagtttagt gtcgtcaaac gacagctgca gtcagctgat
      241 acgattgtga tcgcgactga tagcgatcgt gaaggggagc tgattgcctg gtcaatcatt
      301 caacaagcag gcgccaatca aggaaaaacc tttaaaagac tttggatcaa ctccttagaa
      361 aaagaagcca tttatcaggg attccaacag ttacgagacg cagaagagac ctatccaaaa
      421 tttgaagaag tacaagcacg tcagatcgcc gattggttaa ttgggatgaa cggtagccca
      481 ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gtttttcttt aggtagagtc
      541 caaaccccaa cactctatat gatttatcaa ttgcaggaaa agattagaaa cttccaaaaa
      601 gaaccatatt ttgaaggaaa agcccaagta atcgctcaaa atggcgcatt tgatgcgaag
      661 cttgatccaa atgaaaccca agcgacacaa gaagcatttg aagcctattt aaaagaaaaa
      721 ggtgttcaat taggaaaaca gccaggtacg atccttcaag tcgaaacaga gaaaaaaagt
      781 gcggctagcc cacgtctttt ttctttatcg agccttcagt ctaaaatgaa tcaactcatg
      841 aaagctagtg ccaaagatac tttggaagcc atgcaagggt tgtatgaagg aaaatatcta
      901 agctatccgc gaacggatac cccttatatt acggaagggg aatatgcgta tctgttggat
      961 cacttagacg aatacaagca ttttttaaaa gccgaagcga ttccgactcc tattcataca
     1021 ccgaatagtc ggtatgtgaa taataagaaa gtccaggaac attacgcgat cattcctaca
     1081 aaaacggtta tgacagcagc ggcttttgaa caactaagtc cactgcagca ggctatttat
     1141 gaacaagtgt taaaaacaac ggtggccatg tttgcggaaa agtataccta tgaagaaaca
     1201 acgatcttga cgcaagttca gcagcttcag ttaaaggcaa tcgaaaaggt gccgctagat
     1261 ctaggttgga agaagttatt tggaaaagag agcgacggaa aagagaagga agaagagccg
     1321 ctacttccta aagtgacaaa aggagaaatg gtgacggtgg atctacaagt tcttgaaaaa
     1381 gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcaat gaaaacagct
     1441 gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga aggaatcggt
     1501 acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata tatcaaggta
     1561 gagaaaaata agctcgtggt gacgaataaa gggattttgc tttgccaagc cgtggaaaaa
     1621 gagccgctgc ttacgagcgc cgaaatgacc gccaaatggg aaagctactt gttaaagatc
     1681 ggtgaacgaa aaggaacaca aaccactttt ctagccaata tccaaaaatt tgtctctcac
     1741 ttattggaag tcgtaccagg gcaaatccaa tccactgatt ttggatcgac gttacaagaa
     1801 gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg tccaaaatgc
     1861 caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga agcctgtgat
     1921 tttaaaaggt cagaagggtt catttgaagc aacgattgtt ttaaaagaag acttcactac
     1981 aggctttgaa ttttctgaaa agaaaaaaac gaacttcaag aaaagaacac gccgcactgc
     2041 aaaataatgg agggttaatc ttatgcgtaa taccacacac aaacaacaag aaatcgaaaa
     2101 tcaaaaagcc aaaattcgtt cagaacagga aaaaatgcgt gccagcaaac gaagactgca
     2161 gcaattgaag aaccagcaga ggattcaaaa agaaatcgaa ttagatcgat aaaatcttcc
     2221 taactattgt atacgtataa acttatgtta tactgttttt ataagtttat acgtatacaa
     2281 aggagggaga aaatgacaaa agcaatggta tacaagctgc tagaagaatt taatggaact
     2341 ttggctttga aggatgccaa aaaagcgggc atttctcctg taacgatcaa acgtatggta
     2401 gatcgagggg agctggatcg agagtatcca ggttttttca ccttacctgg tcaatttcct
     2461 gatgaattgt ttatggcgca aaaaaaatat gaacgcggca ttatttcaca tattaccgcg
     2521 ttagatttgt atgatttaac agatatgatc cctagacaga tcgatctgac tgtaccttac
     2581 ggttatcacg tttctgaaaa aggattgaag gaattcgcag ttgagctaca ctatacaaaa
     2641 gcggaatggt atgaattagg taagattgaa ataaaaagtc gctatgggaa tcctattata
     2701 gcttatgatc cagaaagaac tttatgtgac atttggaatc cctggtatac cgttgaggat
     2761 gaaatcaaag taaaagcaat caaaaactat atggagtcgg acagaaaaaa tcttagaaaa
     2821 ttgaatgagt atcgacgaat tttaccaaca gacaaaacga tgcgttctta tattatggct
     2881 ttaaattaaa ggagaagata catgacacct acgcagttaa aagcaaaagt gaagaatatt
     2941 tctagagaaa aagaagttga tccgcagctt gttttaagac attttatgat ggaaaaattc
     3001 ttggaaaaaa tctcagagtc tccttatcgt gaaaattttg tgttaaaagg cggcttcttg
     3061 attggctcaa agtatggcat tgaaaatcgc acaaccaaag atattgatac gactttacgg
     3121 gaaatgaaag taaccaaaga aacattaaca acggttctaa acgaaatttt ttcaacgccg
     3181 acgaaagaag gcattcaatt tgagattcaa ggaatgaaag aaacgagaga agccgattac
     3241 tatccaggat ttagcttacg tgtgctggca catttggaaa acatgcggcc agattttaag
     3301 gtagatgtga ctacaggtga ctcgatctat cctgcaacga tcactcacag tcataaattg
     3361 atgtttgagg atcgaacgat tcctttggaa tcctatccta cagaacaaat tattgctgaa
     3421 aaattaagtg caacctttga ttttttaaca gataattcga gaggaaaaga tttttatgat
     3481 ctctatacta ttcctaaaat ggagaagatc gatgagaaga ccttgaaaga tagtgtgaga
     3541 aatacattta cacgtcgagg aaaaactgat ccgttaaaga tatactatga aaaggatatg
     3601 gatgtactga aaaatagtca cgagctaaaa gatacctgga agaaatatca aaaaagcaat
     3661 ccttatgctg cgtcgatcac gtacgatgag acaatggatt cgattagtaa tttgatggaa
     3721 aaagtgattc acgtcgaaga aaaagagcga aaactagctt atcaaagaca ccaacagatg
     3781 aatagagaaa tggagagata aagatgatta ataatgtgac attagtagga cgcttaacca
     3841 aagatcctga tttacgttat accgcaagtg gtactgcagt agcgactttt accctagccg
     3901 tcaaccgaaa tttcacgaat caaaacggca attgagaagc cgattttatt aactgcgtga
     3961 tttggcgcaa accagcagaa acaatggcga cattagctaa aaaaggtagc ttgattggtg
     4021 tagttgggcg gattcaaacg cggacttacg acaaccagca aggacaacgt gtatatgtga
     4081 ctgaagttgt agcagataac tttcaactat tagaaagtaa agcagcgaca gagagccgtg
     4141 cgcacgctga tcaatcgtcc accagtccaa gcaccgccac ctttgaacaa cggaatacag
     4201 cgacgcctaa caacaatgga ttgaacgctt cacagaatcc atttggtgga caatcaatcg
     4261 atattagcga tgatgatcta ccgttttagt ttgagaaaga aggagtaagc atggcaaaaa
     4321 aaattagtct gacgatggat ctattgatac ccgtaattgc atttatagac gggatttctt
     4381 tgggcgtcac tgaaaatcct ttggtaaata gaattgcgaa aggatcacta tttcttgtat
     4441 tcgtgtatct tgtctgttct tatcttttaa aaagagcagg aaaggataat tccttttggc
     4501 gattgactaa agtcacagta gcagcgacag ctgttatact tacggctatt ctaccgcttt
     4561 atatgtagag ttaacgggaa atcgttgaac ttcttgcgaa atattgctat aatgaacatg
     4621 aaaaagagga gttgcgctaa caacccctca ttaacagcac cgattaaaga acggtggcag
     4681 aatattagtt atttggaaat aaccgctgac ctgctaaagt agacggttat ttctttttgt
     4741 catttttaag catatttaca atcaaactaa tcaaagcaat ggtaaacata ccaaaaccaa
     4801 gaatggtttg aattgtctca tatgcggaca aaaggcttac tcctttcata gattttagca
     4861 cttacattca taagcaccac cacctttcca gaagtcgcca ccgtcttcaa actttactgt
     4921 cttgactatt atagcatatc taaaatcgaa taaaaattct caattttagc tgtttttgct
     4981 ataattttag cgaggtgatc gtgatggaac aatatattgg tttactcgat aaattagggt
     5041 gatcaagaca cagccattgc ttgtgcgatt tacattacgc acgagtcatg aaccaatcaa
     5101 ttgtgtagtg gccaatatag aaatagcgga taaattgctg attttggaag aaggcaagta
     5161 tactatttca gtgacaggtc acttcaataa acgaaatcaa ctagtcattg aatcaatgca
     5221 aattagaaac cctgatcatt ttacgagatt aatgggcata taaataaaag aagagctgca
     5281 gtcctaaatg aggattgcag ctctttttgt tttacataga aacaagaaaa atgaaaaggc
     5341 ctttttagga aggcgcaagc gccttctcaa ttgaaaaacc tctctctcac tccaaccgcc
     5401 accgccgttc gagaagaaag gaagtgaacg ttgcacgttc actaagataa ggggtcttgc
     5461 aaagcaagac aaaatagaaa aaacttcctc tcaccaaaag gttcgttcct gttcgctatc
     5521 tctttattga tttgccagac cggcaacaag ttgcctttta tcaccaaaaa gcgacaagga
     5581 caagggtaaa tggcacgctg cacgcccttg tccttgtcgc ttttagttga ttcattctgg
     5641 ccaacaaatc aataaagaaa gaactaacga aaggaagtaa aacctttcct aagaggaggc
     5701 agaatcaatc atgcgaaaga aatttgatta ttggggtgtt cccttcagcg aattattccc
     5761 aaactatcat gcaccatata ctgtcgaatg cgattgtgga gaaagagcga aatgtatcaa
     5821 aagctattgt ttgtatcaat gtccaacatg cgggaaaaaa tataccttgt cttatggcga
     5881 ttatattttg attgatgaga aaggaaagaa acgctgatga aaacacaaca caaattattg
     5941 actgtaaaag aagtcgttca aattttggtg gacaaagaag tcatttctga aaaagtagca
     6001 gaagctttac ttattccgcg gtactggacg atagaaaccc ctttagtaga ggatgcaccg
     6061 actatgctat ttgaagacct agtgaagatt gcaggatatg aactgcatta tcaagaaaac
     6121 atggaaactt atgaaaacga atattggtta accaaattag agaactaaaa aggagaaaaa
     6181 aatatggctc aaaattacta tgacgaattt gtcaaattac ctttggataa aatggcacaa
     6241 aaaatggagg atatgacatt cctatacaat gagacacgtg ttccaaaaaa acattataag
     6301 gaaagattgt cggtagctgt ggaagaaatg attgaatcgg gtgtcgaaat gaatctcatt
     6361 gcgacctact atcgaacgct tgaagaactg aaaaagcaaa atggacaatg gtttttccaa
     6421 gccttactat gcttggaagc aggcgtcaag ccaaacacta tcaaaccttc tgaataccaa
     6481 gctttagaac taacgtatgg gaaatttgtc gaaacgaaaa aagccaagac tgtttcaagt
     6541 gaatggttgg actacttaga aaatgtcaac aagtatggcg catgttacat catgaaaaag
     6601 gaggacaacg aaaatgaata aggtgattta tcaactaaca acggaagatg tgtcacactt
     6661 tttaacaaac aaagaacatt tgaatgaagt aatcagacgg attcaaaaag actttagttg
     6721 tatggaagaa atgaagtact ttattcaaag agtcgaagaa caattgtatt tggaaagtga
     6781 aacgagtcaa acgaaggtta cagtcagcaa taattattta gacgtgtata aacgtcgtcc
     6841 tgacacatta aaaaaaggag cggagcttct acgactagaa acagaaaaat atatcatgaa
     6901 tgtttaccta gaaaggagtg aactggtcga aaactttgtc agctacacga tttgcaggaa
     6961 acactattac tcagaatcag aatattattc aatttttgag agcatggaat taatggaaga
     7021 gctaaacagc aaagatgcga cggtagacga ttattacctt gtatttacta aatttttgac
     7081 gtgtgggacg gaatctatcg tgttaatggg aatggattat gaactcgctg tacccgaagt
     7141 atttttgagg gaatatgtag aagaagtaga taacccaggc gacgactggt acacgacttt
     7201 aactgatttt ttagatacct acacttacga cgatacagaa gaaatccaaa atgaacttga
     7261 ccgtaaacga atgccatatc aacgatttga aaggaaaaaa ggtcaataac catttatacc
     7321 gaaaagtgag aatgtgtgaa ataaaaacaa aaaaccttga aattaaggga atggttctta
     7381 tttcgcacca atcgctccaa ccgcccccgc cgcttaggtg gtgcgaaata agcgagcaaa
     7441 gcccgaaaac ctaagagaaa gaaggagatt tatatgaaca aaattattcc cttattatta
     7501 gtcctatttg ttggagaaat acttatgcga cttagtgcag ctgtacattt aaacctcgca
     7561 gtatttttgg taatactaat gttttgtttg atcttttggc gatttgtatt agccctcgta
     7621 agtctactat tcaagatgat tgcagccata tttttcatag gattatttcg atgaaaggag
     7681 agagtaccaa tgccaattgc aataggaaat aaacggttac ccgtgacgtt ggatgaaaag
     7741 agacaaaaag aattgcagta accaaagcag aagtacggca aaagtgaatc caggattatg
     7801 tgtattgcgt tagatttatt gattgcgcaa gaaaaagcag gatttgacat accagcactc
     7861 aaaaagtgat cgcactttgc gcacgtgtta atacgtgtta taattatttt gtaaggaggt
     7921 atagtgcaca atgccactta cgggaaaaga aatgcttaag ttattgaaaa aaatggctgg
     7981 gttgagcgaa ggcaagaggg atttcatcat catctttata aggatggtgt tagaatcact
     8041 gttcctgttc atgccaatca agatcttggc cgaggacttg agcgaaaaat tttaaaggat
     8101 gcgggattga aataatctcg cattcgatta aagaaaggat tgactgaaat gttaagtgag
     8161 cctttagcaa aaacctatcc tgctattttt agcccagaag aaggtggtgg gtactttatt
     8221 gaatttcctg atgttcaagg tgcttacaca ggaattaacg aagacgatat atcttatgga
     8281 attgctatgg cacaagaggt gttaggtatg gttttagcag attatattga gcatgaagat
     8341 ctacttccag aaccaacacc aatcaataag atttctgttg aagatgattc ttttactact
     8401 ttaattcgtg tagatgttgc caaatatttg aaagacacag aacttgtcaa aaaaacctta
     8461 acaattcctc aatgggcgga taagctaggc aaaagggctg gcataaattt ttcagtcctt
     8521 ctaacagaat caattgcaga taaagcagat aacattcttc gttctggaag aaataattag
     8581 aactcaaaag ggtcagaaac ttaaaaatct ttggcttctt tttttactac aaagatggct
     8641 tctttaaatt atttgcctga ttgatttaaa aaaattgcgc aaagactacg agctaattaa
     8701 gttgctcgtg gtcttttttt atgaaaactt tttagatc
//
LOCUS       DAJSKS010000111         8708 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJSKS010000111 DAJSKS010000000
VERSION     DAJSKS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.113"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            33..1094
                     /locus_tag="ON396_002184"
     CDS             33..1094
                     /locus_tag="ON396_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR3114248.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1097..1993
                     /gene="murQ"
                     /locus_tag="ON396_002185"
     CDS             1097..1993
                     /gene="murQ"
                     /locus_tag="ON396_002185"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR3114249.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2007..3989
                     /locus_tag="ON396_002186"
     CDS             2007..3989
                     /locus_tag="ON396_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3114250.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4054..4500)
                     /gene="nrdI"
                     /locus_tag="ON396_002187"
     CDS             complement(4054..4500)
                     /gene="nrdI"
                     /locus_tag="ON396_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR3114251.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4711..6135
                     /locus_tag="ON396_002188"
     CDS             4711..6135
                     /locus_tag="ON396_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3114252.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6316..7089)
                     /locus_tag="ON396_002189"
                     /pseudo
     CDS             complement(6316..7089)
                     /locus_tag="ON396_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(7086..7991)
                     /locus_tag="ON396_002190"
     CDS             complement(7086..7991)
                     /locus_tag="ON396_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3114253.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8145..8465)
                     /locus_tag="ON396_002191"
     CDS             complement(8145..8465)
                     /locus_tag="ON396_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HCR3114254.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         2728 a         1570 c         1866 g         2544 t
ORIGIN      
        1 aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata tttatgaacg
       61 attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat ggctttgtcg
      121 gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa aagcgattga
      181 tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat atttcaggag
      241 aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa gcaattggtg
      301 taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac tggtcacatc
      361 aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat gaattgaaag
      421 aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca cgtcccgaaa
      481 cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt caaggtctcc
      541 ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat caaggattgc
      601 caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat ctattagcaa
      661 gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt caggagcaat
      721 ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta gatgaagaat
      781 tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga gatgtcatac
      841 gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat acagctacgc
      901 gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg ggtgagatcc
      961 aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct aaagttatca
     1021 aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa tttataagaa
     1081 aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg aacgcagaaa
     1141 ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac taatgaacaa
     1201 agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg aaccagtgat
     1261 tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg gagccggaac
     1321 aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg gtgtagaacc
     1381 ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag ctgtcgaagg
     1441 ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt tgacaaaaaa
     1501 tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg gtggattaga
     1561 ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac aagccgaaat
     1621 cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt tgacaggctc
     1681 tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt ctactatcag
     1741 tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac caacaaatga
     1801 aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg attacgaaac
     1861 agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg tcatgatttt
     1921 gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg gatttgtaaa
     1981 agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg gaagaactgg
     2041 caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt gtccactgca
     2101 tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct gaattaaaag
     2161 cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc atcggacctg
     2221 ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa ttaggagaaa
     2281 aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa gaccgtgtaa
     2341 acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca tctaaaatca
     2401 aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca tttgtcggta
     2461 ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc ggaacacttg
     2521 atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac gggatgttta
     2581 gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca acaccaactc
     2641 ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa gcaccactga
     2701 aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta ggtgtcatct
     2761 tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta cctgatgcgg
     2821 tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt gaaattttct
     2881 tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc aactgggtac
     2941 ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg ccgatggtta
     3001 tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat gaaactggtc
     3061 gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt gcagcattgg
     3121 cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa ggagctttac
     3181 cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta cctctaggac
     3241 gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc ggaatgatcg
     3301 gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca ttgatctctg
     3361 acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt ggcttcgttg
     3421 ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta gcgactgaaa
     3481 cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct gaaagtgtga
     3541 ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca agtgatccag
     3601 tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac ggacatatct
     3661 attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct attgggatca
     3721 caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt gacttaggcg
     3781 gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat acgcttgtag
     3841 cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg atcgtcttgg
     3901 taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa gcaaaagcaa
     3961 aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac gaaagaagaa
     4021 actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg ctaaaatctg
     4081 ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca agaaaggaaa
     4141 gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga agtttttatt
     4201 tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca gatattcacg
     4261 catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc cgtctaaata
     4321 agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat tttcatggat
     4381 ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt cagcatattc
     4441 tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata aaatgttcat
     4501 ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt ttggtggtgt
     4561 aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg tagaattata
     4621 tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat actttttgat
     4681 aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa aaaaggaatt
     4741 ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg cgtatttggt
     4801 attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg ggtactcgta
     4861 ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa acgtccagat
     4921 ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc tggttttatc
     4981 agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc aacattgttg
     5041 atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct ggcatcgatc
     5101 gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg agtcgaaaat
     5161 gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt tttatttatt
     5221 gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc tagcgacatt
     5281 atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag ttgcatcatg
     5341 gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc acgagcaaaa
     5401 aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat tattatttac
     5461 gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca aacgatcaag
     5521 gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg agcagtcgtg
     5581 atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac gatcttaccg
     5641 attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg gggaaaactc
     5701 aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac gaatgtcttt
     5761 ttactttcat tattattcac tgacagtgct tataactttg cttacacact tggtacagca
     5821 gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc ttatcagcgg
     5881 aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt atgggctatg
     5941 ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc aggcatttat
     6001 ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca cttagataag
     6061 attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt tgccactggg
     6121 atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc tgtcgtttat
     6181 tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc tttttcagaa
     6241 taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt tcacagcctc
     6301 ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta atgctaagaa
     6361 caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat gagtcaattg
     6421 cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga tcggattcca
     6481 tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga ttgacgaagc
     6541 aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa agcaagcaat
     6601 catcaacact aaacttaaaa tcagctataa accgatataa ctacccaaag tataagtgaa
     6661 caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa tcgaagggaa
     6721 aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat aaacagaata
     6781 aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact ttcgataaag
     6841 gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg cagctgcaat
     6901 catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt caactggatc
     6961 aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa caactaatag
     7021 aacagataaa acagcagttg actttttctt gatcaattta aataattctt gttttacaag
     7081 caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag agaagattcc
     7141 aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga caatagctgg
     7201 atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc aggatcttcc
     7261 aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt tgtagagata
     7321 acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt gatgatcttt
     7381 ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag taagtgactg
     7441 gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg cacatctctt
     7501 gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga cggatggttc
     7561 agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga tttcgctttt
     7621 ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc agatacatcg
     7681 ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata gatacccggg
     7741 tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga aatctcattt
     7801 tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc tttcatgatc
     7861 gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg ggataagcta
     7921 aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc ttggaccgaa
     7981 agacttttca aaaatattca ctccttttct atagatagta caagcataca cctgatgagt
     8041 ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa ttgaatatta
     8101 aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg gccgctagta
     8161 atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt ggaaagattc
     8221 agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc aatccctgtc
     8281 cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa aaagtaaaaa
     8341 ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa accatcactc
     8401 catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa ttccatccat
     8461 gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa aatcatccct
     8521 tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat tgtatatctt
     8581 tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt agtaaagtgt
     8641 accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg caatttcata
     8701 gaaagaag
//
LOCUS       DAJSKS010000112         8697 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJSKS010000112 DAJSKS010000000
VERSION     DAJSKS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.114"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            18..497
                     /locus_tag="ON396_002192"
     CDS             18..497
                     /locus_tag="ON396_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3114255.1"
                     /translation="MVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITNDLKLSTSMIHL
                     IDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNKKTKLVSLTKY
                     GRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEYQVSLTRKED"
     gene            590..1752
                     /locus_tag="ON396_002193"
     CDS             join(590..866,866..1752)
                     /locus_tag="ON396_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR3114256.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2032..3012
                     /locus_tag="ON396_002194"
     CDS             2032..3012
                     /locus_tag="ON396_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3114257.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3134..4552
                     /locus_tag="ON396_002195"
     CDS             3134..4552
                     /locus_tag="ON396_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HCR3114258.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4623..6092
                     /locus_tag="ON396_002196"
     CDS             4623..6092
                     /locus_tag="ON396_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3114259.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6102..6569
                     /locus_tag="ON396_002197"
     CDS             6102..6569
                     /locus_tag="ON396_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3114260.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6605..7477
                     /locus_tag="ON396_002198"
     CDS             6605..7477
                     /locus_tag="ON396_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3114261.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7559..>8697
                     /locus_tag="ON396_002199"
     CDS             7559..>8697
                     /locus_tag="ON396_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3114262.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKE"
BASE COUNT         3007 a         1301 c         1761 g         2628 t
ORIGIN      
        1 tagaaagagg tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca
       61 tcaaagtaaa taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc
      121 taaaactcag tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta
      181 gtattacgga attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc
      241 ccactttaga gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta
      301 aacttgtatc gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg
      361 aagagcttta tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa
      421 ccattaaaca gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga
      481 caagaaagga agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca
      541 gaaaaaataa ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg
      601 tcaacgtaga acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg
      661 aaagccccga gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg
      721 ggtcaagcaa gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca
      781 agtagaacta attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt
      841 aaagcaagcg gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa
      901 tactcaatat cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa
      961 gccaaaccag ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc
     1021 aatcgaaaag cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa
     1081 gtgagtcaac gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca
     1141 ttggctcatt tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta
     1201 gacggaacct tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt
     1261 cgtgttggga agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc
     1321 atcggctatt catgcgggga gaagaaagat gcgacgttag tcaaagaagc ttttgcaaag
     1381 attccgtact cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat
     1441 aaccagacga ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa
     1501 ggctgcccct atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc
     1561 gttcatcaat accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc
     1621 cattggtgga ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt
     1681 cgacagcaga gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc
     1741 gaggcacctt aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg
     1801 ttgtcaaggg cagtcggaac accagcagca agcttacgca ataccttata atttttgtca
     1861 aaaaaagtgt tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg
     1921 atattatata tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat
     1981 gtcgaatagt cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa
     2041 attggaatta gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta
     2101 ttgaatgatc gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa
     2161 aaattagaat actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc
     2221 gtaggtttga tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc
     2281 gagttcgagc tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca
     2341 acaaaagaaa aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta
     2401 ggaacgcaca ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt
     2461 gatagaagtc ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa
     2521 ttagctactc aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat
     2581 agtaagataa agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa
     2641 ttcatgctgg aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat
     2701 gaaataatta agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat
     2761 gatttattgg cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa
     2821 gaggttaaag tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta
     2881 tcaactattc aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat
     2941 atgattaagg gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa
     3001 aatacagcat aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga
     3061 aacgggttga catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca
     3121 ctggaggaag aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca
     3181 acctaaggat gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat
     3241 ttatcatcaa cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt
     3301 agcaactacg aaagattttg ttcattttga agactttggg gaatctcttt taagagggga
     3361 tgacaatgag agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt
     3421 tcacgcattt tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt
     3481 gttgcttcat gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga
     3541 gttagaacca caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga
     3601 tgatgaaaaa gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct
     3661 acaaaccgga cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg
     3721 tgatttttgg gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg
     3781 tgactggtgg tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat
     3841 gagtcaaagt ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc
     3901 ttattatgca ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc
     3961 aacaaaagat caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca
     4021 tgaagtctat caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa
     4081 tgcattcata aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga
     4141 agaagtaacg cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt
     4201 tgatgagaat cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc
     4261 ttacgaatat cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta
     4321 cccttggttt caatatatgg ataaaggact tattagacct atatctttaa agcctaacca
     4381 atcttataaa ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat
     4441 cgctcttaac gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa
     4501 tggatcaatg actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc
     4561 aatcatgtgg caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat
     4621 gaatggacta caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata
     4681 tctcaagcgc tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag
     4741 cgaatatgga aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac
     4801 agttacaagt aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat
     4861 tgacaggagc taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag
     4921 gaaatagact tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag
     4981 cttttattgg ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt
     5041 ttggaatgga tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc
     5101 tgggtattat tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa
     5161 aacgttttgg cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag
     5221 gactagccaa tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat
     5281 ttggattaag cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt
     5341 cttggttttt agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt
     5401 acattttagt cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg
     5461 gtccattggg gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga
     5521 agatgggtgc ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag
     5581 gaacacaaca tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt
     5641 atatccaacc tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt
     5701 atttactctt taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa
     5761 cactggtagg tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc
     5821 cttttgtttg ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg
     5881 taaaagccat tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc
     5941 ttccttatat atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt
     6001 atgataaata taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg
     6061 actcaaaaaa attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa
     6121 aaaatgaaat aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa
     6181 gtgatccagt cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg
     6241 aaacgattgt atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct
     6301 taggaataaa aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg
     6361 agctaaatgg tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg
     6421 atattcttat gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca
     6481 ttgttgtttt tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac
     6541 aaggacaaga attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa
     6601 atgtatggtt tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga
     6661 taaagatttg aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat
     6721 gaatttagtt ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg
     6781 atcgttcggt cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac
     6841 gccaaaactt gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat
     6901 tccaactgct tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa
     6961 tggcagaggc ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc
     7021 tatccaaaac ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt
     7081 aaaacgacac aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga
     7141 gggcttagct gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac
     7201 tgaaaatgac gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac
     7261 aacattgatg ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca
     7321 cttaaaaaag aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc
     7381 cgatgttgac aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg
     7441 tttagcttta gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac
     7501 tatttgtttg gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt
     7561 gaaaaaaaaa atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga
     7621 tttttctgct caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta
     7681 tttgaatcag ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat
     7741 gttcaagtat aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat
     7801 atctttgaaa tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct
     7861 cccattaata ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta
     7921 tcagtatgct cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg
     7981 tgcagattat gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg
     8041 agcctttact ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata
     8101 ccttttttta gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct
     8161 tatggattct caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt
     8221 tcgccttatc ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag
     8281 acgcatccca gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat
     8341 tcaattatta tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg
     8401 ttcgattgaa ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt
     8461 gaggaatgaa atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc
     8521 aatttattta ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga
     8581 tttaaaaaaa atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa
     8641 tcacatttca attgaagaaa gcatgagtca attaagagat agaatactta aagagtg
//
LOCUS       DAJSKS010000113         8578 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJSKS010000113 DAJSKS010000000
VERSION     DAJSKS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.115"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(194..628)
                     /locus_tag="ON396_002200"
     CDS             complement(194..628)
                     /locus_tag="ON396_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3114263.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(860..1570)
                     /locus_tag="ON396_002201"
     CDS             complement(860..1570)
                     /locus_tag="ON396_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR3114264.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1560..2420)
                     /locus_tag="ON396_002202"
     CDS             complement(1560..2420)
                     /locus_tag="ON396_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCR3114265.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2425..3063)
                     /locus_tag="ON396_002203"
     CDS             complement(2425..3063)
                     /locus_tag="ON396_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR3114266.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3078..3377)
                     /locus_tag="ON396_002204"
     CDS             complement(3078..3377)
                     /locus_tag="ON396_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3114267.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3420..4904)
                     /locus_tag="ON396_002205"
     CDS             complement(3420..4904)
                     /locus_tag="ON396_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR3114268.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(4924..5385)
                     /locus_tag="ON396_002206"
     CDS             complement(4924..5385)
                     /locus_tag="ON396_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3114269.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5403..6470)
                     /gene="ulaG"
                     /locus_tag="ON396_002207"
     CDS             complement(5403..6470)
                     /gene="ulaG"
                     /locus_tag="ON396_002207"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR3114270.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6688..7458
                     /locus_tag="ON396_002208"
     CDS             6688..7458
                     /locus_tag="ON396_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR3114271.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7731..8558)
                     /locus_tag="ON396_002209"
     CDS             complement(7731..8558)
                     /locus_tag="ON396_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3114272.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2572 a         1823 c         1347 g         2836 t
ORIGIN      
        1 atttgcttta aaaaaaaatc atgtgttcat tttttatatt agttcgaatt tgcaaaaaat
       61 tgtatttgat aaagaggtga gtaaaaaaat aaaatagctg agcattggag gatagattga
      121 gtgatactaa gtctatcctc ttgtttttct aaagaactaa gctagaattt tttcttttgt
      181 acgttattat atgttatttg tcattaccaa aaagcacctt ttttcttcta gtatctatga
      241 cataagaaat accacccatt tcctttactt gaacaaatcc tactgattca taaaattttt
      301 ttgttttctc agtatcatct gtcaataatt cgatttggac tggtccataa ctagtgatct
      361 tctccaaagc ttcattcaaa agcactttgc ctattccttg gcgctgatac ctcggatgaa
      421 tgaggatatc ttgaataaat gcgatcgtcg caaaatcagt gatccaacga acgattccga
      481 taatcttccc ttccccatta cgctttatca gagattcaga ttgttcaaat gctctagcta
      541 accttgcagt gtcttttgtg taattcgtcc aaccaacgct cttatataat tcagaaatct
      601 catcgatcat aagggaatca atattcattt ttttcttcct ttcttataca attctccctc
      661 taataataca gttctttgac tatttaatct atcacatata actctcctgt aaagattctc
      721 tcgattcctt aaaattcctc tttcctttta gaatgcatac tttttgataa ataaaaaggt
      781 tgtgataaat tttcttcaca acctctgttt ctctctatca agtgttatct tttaattttc
      841 tacccttacc ctagcatctc tattgcccgt agtatgcata ctttccatgt ttgcgaaaat
      901 aatgtttatc tagtaaatat tgcgggattt cacatatatt ggggttgatc agctcgtttt
      961 ctttggccat cagacaaatt tcatctaaga tcaaactgtt ttctactgct ttcataggtg
     1021 aatcccccca tgtgaacggt ccatgtccat agacaagtac tccagggact tcgtttggat
     1081 ctaattttct ttccttaaac gtctcgacaa tcacattccc tgtatgaact tcgtaagctt
     1141 ctcttacttc ttctttcgtt aattggcgtg tacaaggaac cttaccataa aaagcatcag
     1201 catgagtcgt gccataagct ggcaaatcgc gtcctgcttg cgcccacatg acggagtgcg
     1261 tggaatgagt atgagtaata gcagttatat cttcaaaagt ttgatacaaa acaacatgtg
     1321 tggggagatc tgaagatggt tttaagctat cttcttctat tatatttccc gacaaatctg
     1381 ttaccaccat ttgatctgct tgcatgcatt catatctgac accgcttggc ttaatgacga
     1441 tgactcctag ctcacggtcc acttcactca cattgcccca agtcaatttg actaatccgt
     1501 atcgcggcaa tgccagattt gcttcaagga cacgttgctt cattgctgca attttttctt
     1561 cacaagacat catagccagc ctccttcaat ttagaactta agtagtcttt tgcctgctgg
     1621 atttctcttc gaaagtcttg gcttttttca ctccacattt caatcagaaa tgttccatca
     1681 taatctagac gtttcaaatg tcttaataat ccaagaaaat caacacaacc gctaccaaaa
     1741 ggaacatcac gaaactggcc tgaatattct tttgttacag ggtaagtatc ttttaagtgg
     1801 atagcagtga tataatcgat ccctttctca agttctcgag ccggattatt ttccggccat
     1861 gcggacaaat tgcctaggtc tggatatact tgtagaaaag gtgaatgaat ctgttctttg
     1921 atttctaaaa attttgaaat ggagttcata aatggatcat ccatgatttc cacggaaaga
     1981 atgatgccat atttagaagc ttcctttacg ctttctttta ttccttcgat aaagtcttct
     2041 ctggaattca ttgatttctc ttcataataa acatcgtatc cagctatttg gattactttg
     2101 acgcttagat tatgcgccaa atggattgct tttttcatta gttgtttcgc tgcttttttc
     2161 ttttctggat cagcagaacc aaacggaaat cgccgatgcg cacttaaaca aatcgaatgg
     2221 atacgtacac cggagctgaa catctcttct ctcagctcag ctatcttttc ttcagaccaa
     2281 tctaatcgtg ctaaccgctc atccgtctca tcaatcgaca tctcgataaa atcgaagtcc
     2341 atttctttta caagggaaaa acgttctttc catgaaatat ctttgggtaa cgctttttca
     2401 taaatcccaa tttgagccat ttccttaccc ccagatacga atgatctcat ctttgaagtt
     2461 ttgtgcggcc ttcatcggat catttgctgc tgtaatacca cgaccagtga taaaggtata
     2521 cacatcgatc ccttcaaata agtgtaatgt gtgtggattt aatccaccag ttacagaaac
     2581 attaaagcct agttcaatca gttttttgat cttcgataaa tctttttcac cccaagtttc
     2641 accagataac agcgcatcac ggctttgatg gtagattacc tgccggatac cgatgttatg
     2701 ccaatccatg gcttgttcaa aagtccaatt gccgtagagt tctacttgca attcacctat
     2761 ttctttttgt gctgcctcca tagttggaat cgttgcacaa caaatgactg tcatccagtc
     2821 tgctcctgct tttgcaacat tactcgccac tgttcctcca gcgtctgcac atttcgtatc
     2881 agcgactagt tttttatctg ggaacatact gcgaatacag cgaatcgctt tttgtccttc
     2941 ctgtaagcaa agaattgtgc ccacttcaat aatatctact acatcaccaa ctttcataac
     3001 atctcctaat gcatgttcta agtcattgtg atctaacgca atttgcaaat tcggcctgct
     3061 catcttagtt ccttccttta cttgtttttt tggtaccact ttgtttcagc aaatttttca
     3121 ctgatttctt tgactgacat cacatttttg ataccgataa ctgtaactcc ttttttctct
     3181 gcctgagtaa acatattcaa aaaattgact ggcgtaaaaa ctacatcgta ttggccagct
     3241 gaactttttc cctctgaaat ggcacaatga tggattttgg taattggtac gtttagttct
     3301 ttcattgctt tttccacact tctcatcatc atcaagcttg ttcctgatcc atttgcacac
     3361 gatactaaaa ttctcatctt aatccctcca ttatgagtgt tgtctaatca gattttcttt
     3421 tatactctct gcttttctgc atatgtgtca tagtcttcta caatcaaaaa ataaccatca
     3481 ggatttttcc gatattgtaa ctgcggaata gccaataaac caatgacaac tacggcaatt
     3541 ccagcataac ctaggacttt catgattact gtaaacaatg gccaagctgt tgcccagtcg
     3601 aacatcccga tataaccacc gtatgttgac atcccaatcc atgtagcgaa taatgctgaa
     3661 ccgcctactt gtatgattcc tgaaagaaac gggaacaggc aagcggcttt gaatccacct
     3721 cggttattgg cataaactgc tataacggca ttatcaaaga acaacggaat gaaaccggct
     3781 atgacgatcg ttggtgattt caataaaatc agtaaaccaa tcatcaaaaa ctgtcccaaa
     3841 gcaccaaaca agaccccaat cgttaccgca tttggcgaac caaagccaaa agtagcagct
     3901 acgtcaattc ctggaacagc tcctggtaaa atcgtgtttg agattccttg gaaagattga
     3961 gttagttcat cgacgaatgt ccgaacacct aattgcaaga tcgccagata taccgcaaaa
     4021 tttaaagaag tcgtcataat gtagaataag aaactctgcc cttcttgcat gaattgcgct
     4081 tgaatcaaat aatctttgcc tagtacaatc agaatgatcc caaagaaaaa tagcatgagt
     4141 aacgaagtag caaccatatt ctcattgaaa atcgacaaga atcctggtaa tttgatatct
     4201 tcaatttttt tgtcttgctt gattgatcta tttttctttt catcccgctt tttcatccac
     4261 tcagctaatt ttgcaaatat aaagattccg aacatttgct gatgtgccac tgcaaatccg
     4321 gcaccttctg tcaattcctg cgtgattcct actgttaagt tagaagctac tgtccaataa
     4381 caacctaaaa tcagtcccat aaaaatcaat gcttgtatcc tacctagatc aggaaagcaa
     4441 aacagtaaaa tccaaaaagc ggtagctgct tgttgaacct gaacgtttcc ggtagtgaaa
     4501 acggaacgca gtttcgtata tttctgaaaa cggactaata agatatttaa aacaaatgca
     4561 atcagtaata aaatcatcac atctccaaag gtacggccaa atgtctccat taatccagca
     4621 tccacagcat tttgtccaaa ataaggatct gtaaccatgg catccaaatt gaaccgttcc
     4681 tttagtccaa ccaagattgg ccgaaaatta ttgaccaaac caccagagcc aacagtcaaa
     4741 ataaagtaac cgaccgttgc ctttaagaat ccagcgatgc attcatgaag cgggcgtttc
     4801 agcaaaatat agcctaataa cacgataaaa ccaatcaggt atgctggctg cgtcaagata
     4861 tttgttgcaa aatacgtcca aatgcttaac aaaaattccc ccatcttttt tctcctctat
     4921 caattaatta tattctgcca tcacaacttg gtaatcatcg atggagttcg tagagatgag
     4981 acgctcaacc aatccatctg tcattaataa ttcggataat ttttggatat tttctagatg
     5041 ttcttctgga tcttttgctg cgagtgtaaa aaacaaacta gccgttttct cctcattctc
     5101 cccttcactt tcaaaacgga ttggctgttt caacttggta aatgaaatag ctgttccaaa
     5161 aacaccatca cttttttctg aagaatgcgg cattgctact tgaggtgcga tcacaatata
     5221 aggaccgtat ttttctacac aatggatgat ctcctccaca tacgtttcat caataatttc
     5281 cttatcgatc aatacaagac agctttcggc gatagcttca cgccaatccg tgatttcttt
     5341 atctgggtaa tgaatcaatt gatggtcaaa aaagtatttc agcaagaaac tccccttctt
     5401 tcttataaaa acgacggaaa cggtgtatca ttttcaatcg agaagacatc ttcaaatcct
     5461 cgataaaaat taaactcaag atcatctttg tttgtcggat agatgaattt cccgcctact
     5521 tgccagatat aaggtttaaa tccatacttc aggcgatcct ttttgaattt ccaaatttca
     5581 gtgatctctt ttggatcagc cataaaatta gaccaaatat cgtagtggac aggaatcact
     5641 acttttgcat tcaatgactc cgccatgcgc agcatatcaa cagaagtaac tttgtctgtt
     5701 atccctcgtg ggttttcacc ataggcccct aaacagacat cgattttgtg ttcattacca
     5761 tgtttggcaa ataaattgga gtaatgtgaa tctcctgcat gataaagatt ccctcctgat
     5821 gtttcgaata aatagtttac tgcaatttga tccatctcct gaggcatttt tccttttaat
     5881 acctcttctg gatcttcaca agtgaccaac gctgtccgat caaacgcttc taaagctact
     5941 acattgatgt ctttgatttt gactgcatca cctggcttta ctgtaatcgt tttttcttca
     6001 ggaattcccc atttttgcca cgtgcgaacc acttcttttg gcccaatgaa tgttgcttca
     6061 ggacaatttt gatgaactgc tgcggctgta tggatatcta gatgatcgga atggatatgt
     6121 gtaacgatta aagcgtctac attttttatc gcaaaaggat cgattacgaa aggctgagta
     6181 cgtaaattag gctgcatatt ttgaacgcca ctcatacgca tcatctgatg gccttttttc
     6241 atcatgccat tcccatgact acgttttccc gtcccacacc atagatcaca taaaatattc
     6301 gttccttcat gacttttcag ccaaattccc gtacagccca gccaccacat agcaaaagtt
     6361 ccaggatcca ctacttctgt ttcgatttct tcattcaagt atgtgcccca ctcaggaaat
     6421 gtttctacta accacttttc ttttgttact tcattgacag ttttactcat ttgcttgctc
     6481 cccccatatc gttgttccaa cttacaagta aatattaatg tgtatcagca tccatgaaaa
     6541 cccttttata tgaccgtttg ttttttatat gattttcttt cagtaatcaa tgattgtttc
     6601 agtataataa agacagatat gaatgtttaa atgaacaact agtcaaataa atccatggat
     6661 caagatgtcc ctcaggaggc aaaagtcatg aaaaactcac ttgtcaatat tgaaaaacgt
     6721 caaaaagaaa ttttggaact gcttcagcag actgaacaac tatcaacttt cgaattagct
     6781 gaatcccttg atgtatctat tagtacgata cgaagagatt taaatttatt agaagaaaaa
     6841 aacgagatca tacgcaatta cggttattgt tcttataatt acaaaaacca gactgatttt
     6901 gatcaatctg gtcctgaaag gattaaacaa gcgattgcca gagaagctag taaatacatt
     6961 aacgattatg atacgctgtt tatcaattct agctcgactg cgttgaaaac actggatttt
     7021 cttcaatcta accatttgac agtcattaca aacaacgtga aaatcaatta ctctccgcac
     7081 aaagataatt gtagttatat tttaactggt ggagagatgc gctttccaaa agaagcacta
     7141 gtgggcgaca tcgcattaaa tacgatttct tcagtcaatg cagacatttg tatcattggc
     7201 tgtagcggag tcgatgtagt acatggggtc acaacaaaga tattaaatga atcaacgatc
     7261 aacgaaatga tggtcaacaa gacaatcaaa tgtaagattt tagtcgctga tcatcgaaaa
     7321 atcggactga catcaaagtt caagattgct gatatttcag tctttgatta tttgatcact
     7381 gataaatttt gttctgaaaa agtattagag gagatcagaa atacggggat aaaaaccatt
     7441 caagtagatt cattttaaga accaggacgt aaatatgtaa ataggattat caaaaaaatg
     7501 aataacaaat ttatcgataa atgaaaaaca cgctacgctc aaattgataa aggaatccca
     7561 agatgtatcc atccaatcga taccatcata atctattgct tcttttataa ccgttatatg
     7621 ttgcctaaat aggaatcaaa caatatcgaa aaaaggaggt atgacaaaag tcacacctcc
     7681 ttttttcgaa taaacggtat tcaaatcaga acactgattt cacaaccact ttatttcagg
     7741 tcgttttcaa tcacctttac cgcgtaatca tttccgttga aacgcgctat tttttttgcg
     7801 tccatatatc tttctaaata atcatcttct cctaattgct tatggcaaga agcaatttca
     7861 taaagcaagt attccaatgc ataagtcgat ttatgtttct tattatactc aatacccttc
     7921 tcacataatt ggattgcttt atcataatcc tcgattttac ggtaataggt ggcgatatca
     7981 taatatacaa tgcagaccat cagattatca aaatcagtct cactcaatat tttaagtgta
     8041 cgctccacat aaactttcgc aaaatctaat tctccttttg acaagtagat cattgccacg
     8101 tttgcagctg cttgtaagtg atggatatct gtttcggtcg tttgttccaa cacttgatta
     8161 aaataaaata atgctgcatc tggttttttc gcataaagat gatcaagcag tccttgataa
     8221 aataaaattt gatttaaaag atcagaatta cttacttttt cctgatcgat cgtttgtaaa
     8281 attttataag attcttggta ttttgataca gaattcaatg attgaatctc atttattttt
     8341 ttcctgattt tgtcttcttc tgtttctact aatatatcgg agatctcgac attcagcttt
     8401 ttgcaaagcg cacttaagat attgatgcct ggcgacatat tcttgttttc gatattgctg
     8461 acagttgctt gttcacaaat gccttctgct aattgtactt gtgttaaccc ctttttcttt
     8521 cggtattctt ttagtaatgc tccatctact ttaatcaaaa caatttcctt ccctttaa
//
LOCUS       DAJSKS010000114         8550 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJSKS010000114 DAJSKS010000000
VERSION     DAJSKS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.116"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..817)
                     /locus_tag="ON396_002210"
     CDS             complement(<1..817)
                     /locus_tag="ON396_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR3114273.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLL"
     gene            963..1766
                     /locus_tag="ON396_002211"
     CDS             963..1766
                     /locus_tag="ON396_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HCR3114274.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1944..2891
                     /locus_tag="ON396_002212"
     CDS             1944..2891
                     /locus_tag="ON396_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR3114275.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2959..3252)
                     /locus_tag="ON396_002213"
     CDS             complement(2959..3252)
                     /locus_tag="ON396_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HCR3114276.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(3280..3945)
                     /locus_tag="ON396_002214"
     CDS             complement(3280..3945)
                     /locus_tag="ON396_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCR3114277.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(4085..4717)
                     /locus_tag="ON396_002215"
     CDS             complement(4085..4717)
                     /locus_tag="ON396_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3114278.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(4724..5791)
                     /locus_tag="ON396_002216"
     CDS             complement(4724..5791)
                     /locus_tag="ON396_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR3114279.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(5788..6519)
                     /locus_tag="ON396_002217"
     CDS             complement(5788..6519)
                     /locus_tag="ON396_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCR3114280.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(6688..7167)
                     /gene="greA"
                     /locus_tag="ON396_002218"
     CDS             complement(6688..7167)
                     /gene="greA"
                     /locus_tag="ON396_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR3114281.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(7303..8478)
                     /gene="mltG"
                     /locus_tag="ON396_002219"
     CDS             complement(7303..8478)
                     /gene="mltG"
                     /locus_tag="ON396_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HCR3114282.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         2538 a         1761 c         1330 g         2921 t
ORIGIN      
        1 ttaataaaga aattatacga ggatatatct tttcaatctc gcttagtagc tgatcttcaa
       61 aatcaatatg tccgtcgaaa ataattatct tttcgctata ctgccaaata tggacatgat
      121 gaacatcctt taccccatcg attgcttcaa tttcttgttc cattttttca tagtcgatag
      181 caggtgcact ttgtatcaaa atagcggcag cttttttgat caccattatc gcttctatca
      241 atatatatat cgaaatgatc agtgtaataa ctggatcgat ccagtagata tcgaaaaaac
      301 ggatcaaaat tgctccgata atcacactta ccgaagacag tgtatcactt aacaaatgca
      361 aataactaga ttttatattc aaactttcat ctgcttcttg tcgtaataat aatactgaga
      421 acaaattcgc gagcaatcca ataactgcta ctgttagcat caaatgacta tttatctttt
      481 ctggtgaagt gaaccgcttg aatgcttcaa cggttaaaaa caaggataat atgatcaata
      541 tgaatgagtt gatgaaagca ctcaatattt ctaaacgctg gtagccatac gtacgttttt
      601 tatttgcttc ttttcgggcc attttttgtg ccccgtaaga aaaaataatt gccaatgtat
      661 cactaaggtt atggaaagca tctgaaacaa gagacaaact ccctgacata atccctccaa
      721 taaattctga taaagtgatc accgcattca aaaccactac tccaaatagt ttttttgatg
      781 atctctgtgg ctgtgtaggc actattcctt cctccatttt atttatattg gatagtcttt
      841 agacaagaat aacatttttt tgatatttca ccaatcattc agctgtttcc ttaaagcact
      901 tgtatttcac ttgatacttc gctagaattg caagtgatga cactactaga tgaggagtac
      961 ttatgacaac aaaacaatta attatcagtt cctttatacg attcaaagac ctaaaggagg
     1021 cgagaaatgt cccattttgg aaagccattg tctatctttt agttttgagt ataattatgg
     1081 ctttgccaat ctcttttcaa gtttttcaag tcttaaacaa cattcaattg gatgggcaaa
     1141 agattgctac tcgcattcct gattttacaa tccaaaacgg gcagcttcaa accgaagaaa
     1201 aagaaggatt catttaccaa acaaactcca ttatcttcac atttgatcca gagggaaaac
     1261 gaacagaaca ggacatctct actgatttga tggggaattt tgttagtgtc gggatgttaa
     1321 aaaacaaact tatcatagct cttcctaata caggcacgac ttctgctttg ttgaacaata
     1381 accaattaga acttccctac accaatgaat ctttaaaaaa cttaacaggt aagcagctcc
     1441 gttctttttt aagtgaagca tctataccac tttggataaa agtcctgaca tttatatttt
     1501 ctatttatcc tagttttctg aatctgctga tcactttgct atttaccaac attgctgctt
     1561 ttctctatgc acgctttcga ctaacaaaag ctacttttct tgattgttta aaaacattga
     1621 tctattcaat ttcattgcct actataattg caacaatctt gatggtcttt cttccttctt
     1681 ttgacactag cgccttcatt gcttttgcag gaatctttat ttttgctcaa gctgtcaaag
     1741 gatgggcaaa aatatcgatc aattaagaaa taaagacaac tcgttatttc tacttcaata
     1801 gaccctttta gaggttgtga catttttttg tcacagcccc ttttttattt gaaaaatatt
     1861 tctttctcaa aaaacaagca aggcgcttca aagttcttct tgtatgcgct acacttagag
     1921 taaagaaagg cgggaaatta actatgtcag aaagaatcat ttatgcagat cgtccatttc
     1981 gtgatgaatt tatcgaagaa atccatacta tagcgcctgg ttatatcttt aaaacagaat
     2041 tacaaccaga ggatctacaa aatgtagaga tcagtttggg ctggaactca gactaccaag
     2101 aaaaactatt agcctcttct accttgaaat gggtccaatc tatctcagct ggtgttgatc
     2161 agctacccct tgagactttt tcaaagaaac atattttact ttcaaatggt agtggcatcc
     2221 acagtgaatc gattacggag catgtcttag gaatcatcct tgggtattct cgtggtcttt
     2281 ttcaagcaca aaaggcacag agtgacatga aatggttagg ttcatcggtc cattaccaat
     2341 ctatccaagg gaaaaaaata ctaatcattg gaactggcca tattggaaaa atgattgcaa
     2401 aaaaatgcaa agcattagac atgaactgct atggaatcaa tacatccggt catccagcag
     2461 aaggaatgga aaagactttt tctttaaatc aactaaagga agttcttcct gaagtagatt
     2521 tcgtagtcaa tatcttacct ctaacggagg aaacaactgg gctctttgat cagcatatgt
     2581 ttgaacactt tgatccaaaa tctgtattca tcaatgttgg ccgtggtgct tctgttaaga
     2641 cacaagatct ggtgcaggct ttaaataacc agcaactttc ctttgcagca ttagacgttt
     2701 ttgaggaaga gcctcttcca caagatcatc ctctttggaa aatggacaat gtactgatta
     2761 cttcccacat tgcaggtttg acacctgatt ttcaaaagaa attaatggct atttttttaa
     2821 ccaatctgaa aagctacttt gccacccatg agcttcaaac caatcaagta aaattgtctg
     2881 caggatatta atagctgaaa acaatatttt caaaagaaaa aagagaaggt gacaaaaatt
     2941 gtgtcaccct ctcttttttt attgtttttt aaagtgatag gttggctcct ccaggttggg
     3001 atcataatca acaagtaaat catatccttt aaagaaccat tcatctgctt cctcgataaa
     3061 aaaaagtgtc ccatcaattt ccactttctt tacaggatgt tctggctgtt caactgacat
     3121 tccaatcgaa aaaccatcat gaacctctgt tttgccgtag acttttccaa aaaaacgtat
     3181 tccgtaagtt tctggcagat caatttctga gtcaaaccac ttttgtgctt ttggcgttac
     3241 cgttaatttc atcccaacaa cctactttct cttttctttc tattgattca tcttatcgtc
     3301 tagaatatcc acatcgcctg tctctccaat aaccagtaga ttatcatttt cacgaacata
     3361 ttcatctgct gcaggagaag cgataaccgt ttgatctact cttcgtatgg cgactactgt
     3421 taagtcaaaa cgttgcctga aatttaattc agctaatgtt ttgttgaaaa atttaggatt
     3481 ggagactcgg atttctgcta aagaatattt atctgacaac tctaggtaat ctaaaatatt
     3541 ctttgacact aaattatgcg caatccttat tcccatgtct cgctcgggat ggacgacgcg
     3601 atcagctccg atcttttcaa gtactctggc atgatactca ttttgggctt ttgctaaaat
     3661 atttggtacg cccatttcct ttaccatcag agtaactaag atactggctt gaatatcttc
     3721 tccaatagcg acaattacat gatcgaaatt tcttatgcct aatgatcgaa gtgtcatttc
     3781 atcctgtgca tttgccacaa cagcatgagt ggctatattc atatattcat tgacccgatc
     3841 ttcattacta tcaatagcca gtacttcttg accagcttcg atcagtgttc ggcagatact
     3901 tccgccaaat cgtccaagac ctacaattgc aaaattttgt ttcaccgttt tcctccttta
     3961 gtatcttctt ttctcagtat aacaaagcct ttacaaaaac aaaagcaagc atcattattg
     4021 taaaaaagcc aagaaaaata atagttattt tctcagcttt tgattatcct ctttatcatc
     4081 gtttttattt caccaaacca tgtttgaatg catagatagc ggcttgtgta cggtcttcca
     4141 cttctagctt agccaatata ttggaaacat gtgtttttac tgttttcagt gtaataaaca
     4201 attcgtccgc tatttcctga ttactttttc cttcagaaat cagcattaaa atttcatttt
     4261 cccgattcgt tagttcttca tgtaatacag gctcatttcg ccgactcatt ttttccatca
     4321 tttttgttgt cacttctggt tctaatactc gctcgccacg ttgcgttgcc cttatagcat
     4381 cagcaatttc atgtgcggta gaggtcttca acaaataccc tgctgcacct gcttcaatgg
     4441 caggataaac tttttcgtca tcaataaaac tggttacgat aatgatttta gcttctggcc
     4501 aatcttttaa gattgctttt gttgattcga taccatccat ttcttccatg actaagtcca
     4561 ttaaaatgac gtccggtcga agtgtcatcg ccttctcata gccttgtcgt ccattttcag
     4621 cttctccaat tacttcaatg tcttcttgaa tagataaata aaaagagacg cctaagcgca
     4681 ccatttcatg gtcatctact aataaaactt ttatcatcct tatctatgcc tccttcatca
     4741 aagggacctt gatttctaca cttgtacctt gacctttaaa actaatgatt tttactgttc
     4801 caccaatacc agctactcgt tctttgatat tgttcaatcc ataacttccc gttttcgttt
     4861 cattcatatt aaaccctgtt ccgtcgtcaa taatacgtaa caaaagatta ttgtctattt
     4921 tgtgcaaata tacctctaat tcattggctt tagcatgtct taatgtattt gataacaatt
     4981 cttgaacgat tcggaacaag tgatcctcaa tggaactaga tagttttaca tcttctacat
     5041 cccatttcag cgaaatctgg attttatttt gcaattcctt caataactgc tcgatacctt
     5101 gctttaaact tttctcttct aaattgactg gacgtagatg aagaagcagc gcacgcatct
     5161 cagactggga agcgttgatg atctctgcta ccattttcaa ctgcttgcga aacatttctg
     5221 gtgtctcgct tttttctgct tgctccgtca atgccgacat catcatcatt gctgcaaaca
     5281 attgttgcga aactgaatca tgcagctcac gggctaatcg atgtctttct aattctaaaa
     5341 tttcttcttt ggtttgtcca tctacatatt ggggacggct cgttacaatt tgtaattcac
     5401 tagatacttc aatcattttt tcttttatct tagtgatttc cttatcaata tctttgatgt
     5461 ataaatcgtc cgatgcactt gggatcggaa gaagcaacaa tttggattca taattgccag
     5521 aagaaagtgc acgcagcttt tcttcgattt tcccgtattt tgtcttttga ataagtgata
     5581 ataatagaaa gacaattaat cctacaccta aggaaatggc taacaaatgg aagataagcg
     5641 gaacatataa taaacgtgca cgcagtaatt ctccccacca atgactttga ttgctggcat
     5701 aaaaataagt gaaaagtgaa aatagaataa taaggaaagc cgcaatcccc gaataaacag
     5761 ctagcatcgc tctggatatt tttcccatca tattcgtatc acctcgacat ctccaatcaa
     5821 ggtattagta ataatcttca atctacgagg gttttcatca taatcactgc tgtagatttt
     5881 gatttgttca tttttcaaag aaaattgctc ttcttcaaac aagacatttc ctactaatgt
     5941 cgcatgctct aatcggatgg ctacgcccaa aggtactaag atccgtgttc tgcctatccc
     6001 ttttcttaca atcacaatat tatcatcttt tggcaaaagc gtattgccca aatctatgat
     6061 agtatcacca gaaatgacgg cgatgttgat atcgtcccat tcatagacgt cgtttccaat
     6121 acgttggtta ccgaataatt gctgtctttt ccgctcatta ttatgtgttt ttaattgctc
     6181 tgtttggatc atcatgatct gtttctttcg ccaaaaagca tttttggtta aatctatccc
     6241 tgaaatctct acacctttca gaccaataaa taaaacggca aatacagcca tcatccataa
     6301 agcaggactg ttgactaagc taaaaaaaat gaccaaactt cctaatatca attgaaaatt
     6361 ttgaaattta gtacgaggat gtttgcgcat agctagatat atattgaaaa taccgaatat
     6421 cactaaaagc aaaagtccag tattgtttac gatttgccaa acagcaaata acagcagtag
     6481 tgcttcaact accacaaaaa aacgccaaga actattcatc gctctttctc cctcctgaca
     6541 cattctcatt tcatcatacc tattttttta actgttggct ataggacttt agatgtaaga
     6601 tcgtttgttg aaatctaaaa tacacaaaga gaaaaagatc tagagtgtat catcagtaag
     6661 atgaactctc tagatctttc agtcagacta cgcttgttct actttgacga ttttaactaa
     6721 catatcgcca cctggagtag caatagttac ttcatcattt ttacgtttgc caatcaatgc
     6781 ttgcgcaata ggagaatcat tggaaatttt gccagaaaac ggatctgctt cagcgcttcc
     6841 gacaatcgtg tactcttctt cttctccatc tggtagttcg atgaatgtaa ctgttttacc
     6901 aattgatacc tcatctgcat ctacaccgtc gttatcgatg atttgagcaa aacgaatcat
     6961 attttcaatt gttgtgattc ttccttcaac aaatgcttgt tcatctttcg ctgattcata
     7021 ttcagagttt tcggaaagat ctccgaaacc gcgggcaatt ttgattcgtt caatgatttc
     7081 tccgcgtttg actgttttta gttcttctaa ttctttttct aatttttctt taccttctag
     7141 agtcattgga taaactttct ctaccatgtg taacgtctcc ttttttcgat aaaataccgc
     7201 aaaaggtaat cttttgagga atatagtcca aaaaagatta ccacgctttt cttcaaatgt
     7261 aaatgttttt ctaaaaatga tcaatgtcta gagcctgttt ttctaagaat tatttacata
     7321 tttttccact aattccatat gttcttcata agttttagcg aaataaacat ttccagtgct
     7381 cgtatctgct acgaaataat aatagtcatt ttctgctggt tctaatactg ccttgattgc
     7441 ctcttcgctt ggattgtcaa aaggtccagg tccatagccg gtattggtat acaaattata
     7501 tggtgaatct acagctgtat cttcatacgt cactaattct ttgtgttcac ctaatgcgta
     7561 taaaacagag atatctgact gaagtggcat atttgctttg attcggttaa agaagacttg
     7621 tgcaatgttt ttacgatcat tttcttttac gccttctttt tcaactagag aagctagagt
     7681 taaaacttct tgtacagtca aatttttctg cttgatagct gaataatagt tttgcatgac
     7741 cgtattcgtc ttgttcacca tttcaatcac tagatcctta agcgaagtac tcgtgtaata
     7801 atcatatgtg gctgggaata agtaaccttc caaacggtat tttgtgttct cagctttaga
     7861 tgcactttcc aacagttctg ggaatttttg gtgcaactcg ttaaagaaag tctcatcatt
     7921 cactaaatct aaaaattctt tcttgtcttt accagttacc ttcgctaccc gttcagcaat
     7981 ctgatcgata tcgtaccctt ctggaatggc aattttccca tctgcaactt ttgttggctc
     8041 agaagtaccg ccttcttgca actgtttgcc aatctcgtca agagtcatat taggcgctaa
     8101 ctgataatag ccagcttgaa agccagttaa atttttaaat tttgtataat aattgaaaac
     8161 aatcccgctt ttaataatat tgtccttctc caaaatctca ccaatctgct tgttagaaga
     8221 gccactcggg atctcgactt ggactaattg gtcatctgat ttatctaaag gcttcaaccc
     8281 gctatctaca taacgataca cggtaaatcc aagaaatccg ccaataatca ataatgtaag
     8341 agcaatcact aaaatgatct tcctcacaat cttatcttct tttttacgca gatttctccg
     8401 cttgtcgatt atatctgctg tagctgcctt actggcttct tttttatctt tacttgggtg
     8461 ttgtttgttt cctgacaaaa caactcctcc ttactatcaa gttcgtaact ttattcatta
     8521 tacatgatat ttgaattggc gtgaatgatt
//
LOCUS       DAJSKS010000115         8494 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJSKS010000115 DAJSKS010000000
VERSION     DAJSKS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.117"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..173)
                     /locus_tag="ON396_002220"
     CDS             complement(<1..173)
                     /locus_tag="ON396_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3114283.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="ON396_002221"
     CDS             complement(593..1714)
                     /locus_tag="ON396_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR3114284.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ON396_002222"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ON396_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCR3114285.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ON396_002223"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ON396_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HCR3114286.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ON396_002224"
     CDS             complement(4304..5215)
                     /locus_tag="ON396_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3114287.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ON396_002225"
     CDS             complement(5232..6239)
                     /locus_tag="ON396_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR3114288.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ON396_002226"
     CDS             complement(6236..8365)
                     /locus_tag="ON396_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR3114289.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="ON396_002227"
     CDS             complement(8370..>8494)
                     /locus_tag="ON396_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3114290.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAJSKS010000116         8355 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJSKS010000116 DAJSKS010000000
VERSION     DAJSKS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.118"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            112..2721
                     /gene="clpB"
                     /locus_tag="ON396_002228"
     CDS             112..2721
                     /gene="clpB"
                     /locus_tag="ON396_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR3114291.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2872..3558)
                     /locus_tag="ON396_002229"
     CDS             complement(2872..3558)
                     /locus_tag="ON396_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114292.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3536..4030)
                     /locus_tag="ON396_002230"
     CDS             complement(3536..4030)
                     /locus_tag="ON396_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCR3114293.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4050..5270)
                     /locus_tag="ON396_002231"
     CDS             complement(4050..5270)
                     /locus_tag="ON396_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3114294.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5417..7252
                     /gene="lepA"
                     /locus_tag="ON396_002232"
     CDS             5417..7252
                     /gene="lepA"
                     /locus_tag="ON396_002232"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR3114295.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7448..>8355
                     /locus_tag="ON396_002233"
     CDS             7448..>8355
                     /locus_tag="ON396_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114296.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYG"
BASE COUNT         2832 a         1504 c         1690 g         2329 t
ORIGIN      
        1 gtcaaaaaac tatgtgtata ttttgaccaa aattgaccaa agcgatataa tagtttttgt
       61 aaagatcaaa gaaataagtg atcttacctt taattaggag ggtatacctg tatgaatatt
      121 gagaaaatga ctacgacgct tcaagaagcg attgctgaag ctcaacagat tgcggtcact
      181 cgaaaacatc aagatatcga cattgcccat gtatggaaaa tctttttaca atctaatcat
      241 tttggtcgaa atttctatac ggatgccgga ttggatgtcg aatcttttga acatgaaatt
      301 gatcgtctac tggatgagta tccagttgtt tctggtggaa acgtccaata tggacaaaac
      361 ctcagccaga atttctttcg attattgaat gaagcagacc aaatacgtga aagcttcggc
      421 gacgagtttc tttcaacgga agtcgttatt ttagcgttga tgaaattaaa aaattatccg
      481 ttaactgttt atttgaataa aaacggatta tctgaaaaag aattgcgtaa aaacatcgaa
      541 gagatgagag gaggagaaag agtgacctca caaaaccaag aagaacaata caaagcatta
      601 gaaaaatacg gcgttgattt agtacagcaa gtcaaaagcg gaaaaatgga tccaatcatc
      661 ggtcgtgatg aagaaatccg tgatgtcatc cgtattcttt cacgaaaaac aaaaaataat
      721 cctgttctga taggagaacc cggtgtaggg aaaacggcaa tcgttgaagg gcttgctcaa
      781 cgtatcgtac gcaaagatgt gcctgaaaat ttaaaagata aaacgatttt ttcattagat
      841 atgggtgcat tgatcgctgg agcaaaattc cgtggtgaat ttgaagaaag actaaaagca
      901 gtcttgaaag aagtcaaaaa aagtgatgga cgtatcctct tgttcatcga tgaaatccac
      961 aatatcgtag gggcaggaaa aacagaagga agtatggatg ccggaaatct gttgaaacca
     1021 atgcttgctc gcggtgaatt acatttgatc ggtgccacaa ctttagatga gtaccgtcaa
     1081 tatatggaaa aagacaaagc gttggaacgt cgctttcaaa aggtattagt caaagaacca
     1141 acagtggaag atacgatcag tattctgcgt ggattaaaag aacgttttga gatccaccat
     1201 ggtgttaaca tccatgacaa cgcattagta gcagcagcta ctttatctga ccgttatatt
     1261 actgatcggt tcttaccgga caaagcaatc gatttgatcg atgaagccag tgcaacgatt
     1321 cgggtagaaa tgaactctat gccaacggaa ttagatcaag ttactagacg tttgatgcag
     1381 ttggaaatcg aagaagctgc actgaaaaaa gaatcagatg atgcaagtaa aaaacgcttg
     1441 aaaaatttgc aagaagaatt agctgaactt cgtgaagaag caaatgcgat gaagatgcag
     1501 tgggaaacag aaaaagaaga agtcaattct gtttcagcaa aacgtgctga aatcgataaa
     1561 gcaaaacacg aattagaaga tgcagaaaat aactatgatt tagaacgcgc tgctgtattg
     1621 cgtcatggta cgatacctca attagaaaaa gaattgaagg aattagaagc taaagcaaaa
     1681 gacagcgaga ttaagatggt tcaagagtcc gttaccgaaa atgagattgc tcaagtcgtt
     1741 ggcagattaa cgggtatccc cgtaacgaaa cttgtagaag gtgaacgaga aaaactaatt
     1801 aaattgaatg aaacacttca taaacgagtg attggtcaag atgaagcagt tgatgcggtc
     1861 agtgacgcgg taattcgttc acgggctgga ttgcaagacc caaatcgtcc tctaggttct
     1921 ttccttttcc taggaccaac tggtgtcgga aaaaccgaat tagcgaaagc gttagctgag
     1981 aacttgtttg actcagaaga tcatatggta cggatcgata tgagtgaata tatggaaaaa
     2041 catgctgtgt ctcgtctagt cggagctcct ccaggctatg taggttacga agaaggtggt
     2101 caattaacgg aagctgtcag acgaaatcct tatacaatcg tcttgttgga tgaaatcgaa
     2161 aaagcgcatc ccgatgtctt caatatcttg ttacaagtcc tagatgacgg acgcttgact
     2221 gattcaaaag ggcgagtagt agatttcaaa aatacggttc tgatcatgac cagcaatatc
     2281 ggttcacagt tactgctgga aggcgtgact gctgatggaa cgattccaga agcagtggct
     2341 gaacaagtca acacgctatt acgtggaaac ttcaaaccag aattcttgaa ccgaatcgac
     2401 gacacgattt tatttacacc attgagccta gacaacgtga aaggaatcgt tgacaaaatg
     2461 gtggcgcaat tggcacaacg tctggaacat caagaaatcc tgctgacaat cagtgatgaa
     2521 gccaaaacgt ggattgctga aaatgcttat gaaccagcat atggtgcaag accattgaaa
     2581 cgatttatca ctaaggaagt agaaacacca ctagctaaag aaatcgttgc aggacatgtt
     2641 atgcctaaat caaaagtaac gattacttta cttgatggac aacttcattt caaaacagaa
     2701 gagctagaag aaatcgtcta atcttccatt gagactataa aagaaaaaag gggtctgaca
     2761 caactagtgt cagacctcct tttttcaaat aaacagtgtt ccagaagtag tttcttcgaa
     2821 aaataagccg aaatttcttg aagctgcccg cttctgtcac aacctctttt attaggtatt
     2881 tttcactttt tctaatttat aactttcaaa gtaacgctca tcttttttac tttgagacca
     2941 tgcaaacaat cccattaagg cagcacctat cgtatttgtc agcaagtcgc ccatcgtatc
     3001 catcaatgct gctcgtccga caaacagtgt gccatcagaa gcagcaaaac gttgaagatt
     3061 catgcctaag aactggtcgc ataagaattc ccagaactcc caaaacacgc cgcaaagtcc
     3121 agcaaacgca aagccgaata agaggaacaa ccaaggactg gtttttgata tctcagcatc
     3181 tttcatcaag taaccaatca atccatagcc aagagctgtc aggaccattg gactcaccgc
     3241 atgtaagatt ttgtcccaaa aagagatgat actgatcaaa tgcatccctg tgcctaaaaa
     3301 gacagagatg aacaagaaaa accagtaaaa caaaacgatt gctgctggta ttttaagttt
     3361 gaaaactttc aaaatgactt gtggcatgaa aatcaatgca atccctaata aagattccac
     3421 gataaatgtg attccttgag aagtcgaata tttttgtgtg ataaattcat aaaaattata
     3481 gcagacaacg aatacaccaa aagcaagtaa agctatttgc acctttttga tttctttaat
     3541 cactgaaagt ccctccatct aaaaatcttt ttacttctga aagcaactca atttctgaag
     3601 aaaccacttt tccgttcaat ttgatcaaac ctattgtata taagttcagg tagtgaaatt
     3661 gattttcagc tgtttcttgt agtgcagcga tttttttcgg gtgggttccg ccttgctgtc
     3721 gagtatcagt atataagccg atcactggga tgcctttcgc ataagccaca ccgatttccg
     3781 atgctacccc ggcatcgatc gtcagcccat ctagtaaagc aatcattaaa tcactagcca
     3841 acactttttc tgtgtcagca agagcaatca ttcgactgtc tgcataagca gttttgtcat
     3901 tgattgcttc attttcttga ggaagataga tcgaaagctc agaagagagc tctctgattt
     3961 ctttaactaa ctgttggtta tataacaaat cacttttagc aaacattggt gcggcaaaat
     4021 aaattttcat ctaaacatca tcctttttat caaaaataat cgctaaagta agcttccatg
     4081 tttggaatga tacgctctag tgacttcagt gtttcttcat ctgtttctaa ccgttcgatt
     4141 cttgccaaat aagaagcacg gcgataattc atcatcaagc aagtcaatgt ttggatatcc
     4201 agtcgaacgg attttcctat aggttcattt aagacacgga cttgtccctg ttcatcccat
     4261 gttaaagcga agacaccatt attccaagga gcgacaggat cttcaatgat gaaatgaaaa
     4321 gcatcagctg ttccaacaaa cggaaaccgt tgaaggaatt cttttacatc aacgatccga
     4381 gccataaaat atggttcgat ctgctctttg atctgactat cttcaatcaa aaatgctaaa
     4441 ggctcatttt tataaatatc tccatgtacc cagtagatca tggaaaaatg tgcggaaata
     4501 aagttccata agccatttcg agcttcttga tcaagataga acatttcttt tacgtgaaat
     4561 atttcttcag ctacccagta gaacagaaca cctcttggtt catggttgct atcgtagtag
     4621 acagcggctg tgcgctcatc ttcattttcg aaacgccaat actcttccca atgaaaatca
     4681 ctgcggaata aagcaccatg gttttgttgg gcaaatttgt cataaacgga aaatacgtct
     4741 ggatgatcaa ctggtaatcg ctcaaccata ccacttactg ggatttgttt aggtagctgt
     4801 gtatctctga ttttaaagga aagtttatca gacatgattt cccatccttt acgccgatag
     4861 taggggatac tgtaaggata caaataagag atccattgct tattcttgcg catattatcc
     4921 aatgcaacga taattagatc ttgcatcaat ccatggttag catattctgg ataagttcca
     4981 actccagtca caccacccat tttataacgt gcaccgtgaa tattgacttc acaaggatag
     5041 atagcgattt gagagatcag tttattttcg tggaaccatc cgaacacttt cgatacttcc
     5101 aaaatcggtt ttttggaacg gataaaagct cgtttattct caaacccgct ttcctcgatg
     5161 tctgcttcag tcacttggaa cacataggaa agcaattcat tgaattgatc gatatgttct
     5221 tgagtgaccg gttttaattc aagattttct ctaaaatctt tttgatccat tttttcatct
     5281 ccatttatta gcttgataac agtatagcaa aattctacaa aaaaaacttc tatagctgac
     5341 taattaaaaa ctctttaaat tactgctttt cgttggtata atggaaagtg ctgattatat
     5401 gaaaagagga gacgctatgg atataaagaa aatgaaagaa cgccaagagc agattcgtaa
     5461 tttttcgatc atcgctcata tcgaccatgg gaaatctaca ttggccgatc gtattttaga
     5521 aatgacgcac actgtgacgt ctcgcgaaat gcaagatcag ctgttagact cgatggattt
     5581 ggaacgtgaa cgtggaatca cgattaaatt aaatgctgta gaactcaatt acacagcgaa
     5641 aaatggagaa acatatatct ttcacttgat tgacacccca gggcacgtcg acttcaccta
     5701 tgaagtctct cgaagccttg ctgcctgtga gggtgccgtt ttagtggtcg atgcagcaca
     5761 aggaatcgaa gcacaaacat tggccaatgt ttatttagcg ttagacaatg atttggagat
     5821 cctgccagtc atcaataaaa tcgacctacc cgcagcagat cccgaacgtg tccgtcaaga
     5881 aatcgaagac gtaatcggaa tcgatgctag tgaagctgta ttagccagtg ccaaatcagg
     5941 gattgggatt gaagatattc tcgagcagat cgtggaatat gttcctgcac caagtggaga
     6001 cttagatgca ccgctaaaag ctttgatctt tgactctatt tatgatagtt accgcggtgt
     6061 tgttctaaat gtccggatca cggacggtat ggtcaaaccc ggagacaaaa tcaaactgat
     6121 gagtaatggc aaaacatttg atgttacaga ggtcggggtc ttctcgccaa aagcagttgc
     6181 tagagacttt ttgatggtcg gtgatgtcgg ttatatcaca gcaagtatca aaactgtcca
     6241 agatacacga gtaggggata cagtaacgtt agcagacaat cctgccgctg aagctttacc
     6301 aggttaccga aaaatgaatc cgatggttta ttgtggtctt tatccaattg atacttctcg
     6361 ttacaacgat ttgcgggaag ctttagaaaa actgcaattg aatgatgctg ctttacaatt
     6421 cgaaccagaa acctctcaag cattaggttt tgggttccgc tgtggattcc tcggacttct
     6481 tcatatggat gtcgtccaag aacgcttgga acgggaattc aatctagaat tgatcacgac
     6541 tgcaccatcc gttatttacc atgttaataa aacggatggt acgactgcta cagtagataa
     6601 cccggcagat tttcctgaac ctgtaacgat tcaggatgta gaagaaccat tcgtcaaagc
     6661 acagatcatg gtcccaaatg attttgtcgg tgcagtaatg gaactttctc aacgaaaacg
     6721 gggagaattc attacgatgg attatttaga tgattaccga gtaaatgtcg tctataatat
     6781 tccgctttct gaaattgtct ttgacttttt cgacaaattg aaatcaagca cgaaaggcta
     6841 tgcctcattg gattatgaaa tgtctggtta tcaaaaaagc aagttagtga agatggacat
     6901 cttgctgaat ggagaaaaag tcgatgcatt aagctttatc gttcaccgag actttgccta
     6961 tgaacgtgga aaagcaatcg ttgaaaaact gaaaaaacta atcccacgcc aacaatttga
     7021 ggtgccaatc caagcagcta ttggacaaaa aatcgttgcc cgttcagaca tcaaagcctt
     7081 gcgtaaaaac gtcttggcta aatgttatgg tggagatgtt tctcgtaaac gtaaactctt
     7141 agagaaacaa aaagaaggga agaagcggat gaaacaaatc ggctccgtcg aagttccgca
     7201 agaagccttc atggccgttc tgaaaatgga tgagcaagat agtgtgaaat aacttataat
     7261 caatcgaaaa acctattaaa taaacctttt tgagtagatg caacactgag gattatctgt
     7321 ataaaagatt ttaaactgtc atttggttat cacggcgata ctaaaattaa caaaattgga
     7381 atatttttct tgaagatttt tagctgtcag tatatgatga aagcatctca taggaggtgc
     7441 ttttttcatg gttgataatt caagtggaag ggttcttaaa tctctaagaa aagagaaaaa
     7501 actatctcaa aaaaagttag ctgatttagc aggtatttca caaagtactt tagttaaata
     7561 tgaaaaaggg tcaagaaaaa tccctaaaga cgtagataat actctaagca agattctgaa
     7621 tatagaaact ctaataaaag atgaggaaga taagattgag attctcatcg gtaaactaat
     7681 tgcatacaga gatatgaata agttacttaa caaagagtta gctgataata taggaattag
     7741 tgaagtatta ctcagttatg tgttaaatag aaagagaaat ccgtcaaaag aaatgcaaaa
     7801 aaaaatcgat atttttctat taagtaatga aaaagaaatt ttgaaggaga ttaatcgtga
     7861 tagtgagata tttagtttat caaaagacga taaaattgtt atgggaaaaa gaatacgaga
     7921 agtccgaaag aaccgggaag agacgcttga aaaatttggt aaaaacttta ccatctatac
     7981 aggcaaaaat gttataagta gatgggaaaa aggtattaat attccagata ttgaaaaact
     8041 aatgaatatt gcctatcttg gtaaggtcac tgttccttac cttatgtacg gagaagatta
     8101 taagaatata cttccaaaag atgaaagagt tagcgatttt aaaaagataa attcttttag
     8161 tatgggatta aggatgagga aaattaggaa agattattat ctagaaaggg aggagtttgg
     8221 aaagcttttt tctccttcaa tttcaaaatg gtccatagat aggtatgaga atggtagaga
     8281 tatacccaat actaatcgaa ttatccaata tgcttatata ggaaatcttt ctttagaatt
     8341 tttgatttac gggat
//
LOCUS       DAJSKS010000117         8260 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJSKS010000117 DAJSKS010000000
VERSION     DAJSKS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.119"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            166..1038
                     /locus_tag="ON396_002234"
     CDS             166..1038
                     /locus_tag="ON396_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3114297.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            1035..2993
                     /locus_tag="ON396_002235"
     CDS             1035..2993
                     /locus_tag="ON396_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3114298.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3325..4176)
                     /locus_tag="ON396_002236"
     CDS             complement(3325..4176)
                     /locus_tag="ON396_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3114299.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            4299..4766
                     /locus_tag="ON396_002237"
     CDS             4299..4766
                     /locus_tag="ON396_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCR3114300.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(5029..5511)
                     /locus_tag="ON396_002238"
     CDS             complement(5029..5511)
                     /locus_tag="ON396_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCR3114301.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(5611..6474)
                     /locus_tag="ON396_002239"
     CDS             complement(5611..6474)
                     /locus_tag="ON396_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR3114302.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            7072..7947
                     /locus_tag="ON396_002240"
     CDS             7072..7947
                     /locus_tag="ON396_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114303.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(8092..8244)
                     /locus_tag="ON396_002241"
                     /pseudo
     CDS             complement(8092..8244)
                     /locus_tag="ON396_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
BASE COUNT         2546 a         1674 c         1483 g         2557 t
ORIGIN      
        1 tgattacgtt aacaataatt cgtttcatgt gactagatca tactaggcat gaaaaatctc
       61 tgaggaacat ggtttcccta ggaggttttt cgtgtctatt tttgtttggc cgcaatcaac
      121 tggtctagca aacgtgaaaa ccaaatcttc aaaaagggtg cgcgcatgaa aatcaaaaag
      181 atctttaatc aaaatgcagt attggtagat gacggtggac aggaaaaagt agctattgga
      241 aaagggattg gatttgacag aaaaagaaat gatttgattt ttgatcgtga tgtcgaacgg
      301 atatttgtca tggaaccaga aggtcaaatc aagttgcaaa atttacttaa tcagattgat
      361 gaacgctttt tatttgcagc agaaacgatc attgatcatg cggaaactgt tttgatggaa
      421 aagttgaatg aacatgtatt gatcgctttg accgaccaca ttgctttttc tgcggagaac
      481 attaataacg gtatcgtcat ccgcaacaaa cttttaagag aaatcgaagt gctttacagt
      541 gatgaattca gtattgctca atgggcagta gagtatctga caaaagagct tgggattcca
      601 tatacttatg acgaagcagg atatatcgca atccatattc acagcggacg aagcggtcgg
      661 aacaataacc atcgcagtat ccgtgaagtg acgattgtat ctgacatcat ccatttagtg
      721 gaatcagaat tggaaacaga tatccattca gaaccatttt cactgaatta ttcaagactg
      781 gtgaatcatt tacgactgct tctacaacga acccatgcgc aacaatatgc tgttttagac
      841 acggaaatcg tagaaatggt caaacgaaaa tacccagaaa gctataagat ttctaaaaaa
      901 atccgtgtgt tgctgaccaa agaataccag ctcgcgatca cgaaagaaga actgggttac
      961 ttggccatcc atatcgagcg tttaagaagc gcaatcgtac aaacaaatgt taacaatcat
     1021 gaggaggaaa aaaattgaaa gcatatctac aacgaatggg acgttccctt caacttccag
     1081 ttgcggtctt acctgctgct gctttactag tagggatcgg aaactggtgg gcatcttata
     1141 gtaacgacat tgtcgctcat tttttacaag ctggcggtaa cgcagtttta ggtcaattac
     1201 ctttgctgtt tgccgttggg ttagcactag ggatgtcagt ggacaaatca ggtgctgctg
     1261 ccttggcagg tttggtcgca tttgaagttc cagtaaatgt tttaaaaact gattcagtag
     1321 ctactttatt gaatatcaaa ccagaatcag ttgatccatc ttttgctcag atcagtaacg
     1381 tatttattgg tattattgcc ggattgattg cggcagctct ttataatcgt ttccacgaag
     1441 caaaattacc gatggcttta tctttcttta gcggcaagcg tctagtgcca atcttggctg
     1501 cctttgtcat gttgctgatc agtgcagtgc tactgctcgt atggcctccg gtttactctg
     1561 ctttagtttc ttttgctaaa ttcattttga gtttaggctg gggtggtgca ggtctttatg
     1621 gcttcttcaa ccgtttactg atccctacag gattgcatca agcaatgaac tccgtgttct
     1681 ggtttgacgt agcaggaatc aatgatattg gtaaattttt ggcttctgaa ggaacaaaag
     1741 gcgtaacagg aatgtatcaa gctggatttt tcccaatcat gatgttcggg cttccagcag
     1801 gagcatatgc tatttacaga aatgcacgtc cagaaaacaa agcaaaaaca gcttccttga
     1861 tgttagcagc agcttttgct tcattcttta ctggtgtaac agaaccactt gaattctcat
     1921 ttatgttcgt tgcttggcca ttatatgtct tgcatgcaat tttcactggg atttctttag
     1981 cagtgagtgc attcttccat tggacagcag gatttgcctt tagtgccgga tttgtcgatt
     2041 acttcctatc tttgaaaaat ccgattgcga atcagccatt gatgttgatc gtccaaggat
     2101 tagtaacagc agtgatttac tactttggct ttaactttgc tatcaaaaaa ttccatctga
     2161 tgactcctgg ccgcgaggaa gcagatcttt cagatgacga tactgcaacg actaattcag
     2221 acaataaata tgcggcacaa gcaagcaaaa tctacgctgc attaggcgga gccgacaatg
     2281 taacttcgat cgataactgt actacacgcc ttcgtttgca agtcaaagat acaggcctga
     2341 tcgatcaaaa taaaatcaaa gccactggcg tacctggtat gaagatcatc gacggaaaaa
     2401 atgcgcaagt catcgtaggt acagaagtac aattcgttgc agatgagatg gcaaaactac
     2461 atggtggtgc tgcaacacga cctgcacaaa caaacaccgt ggttaaaaca gaaacatttg
     2521 ccggtggaac gatcgaacaa gatatttacg ctatcgcaaa tgggaaactg attccaatca
     2581 ctgaagtttc agatgatgta ttcgcggaaa aaatgatggg tgacggctat gctgtattac
     2641 cagaaaatgg agaaatcttc tctccaatcg ctggtacgat cacaaacatt ttcccaacga
     2701 aacatgctgt cggaatccaa acagatgctg gtatcgaagt cttgcttcat atgggcatca
     2761 acactgttga cttaaaaggc gaaccattca ctttatatgt aaaagaaggt caaaaagtcg
     2821 caagaggtca attgattgct ttagttgatc ttgccgctat tcaatctgca ggcaaaaaca
     2881 cggatatggt cgttgtattc acaaatggcg acaaagtcca aagcttagaa atcgaacccg
     2941 cacgtgatgt aaaagcaaat gataaaatcg gctctgtttc caacaaagcc tgagccatcg
     3001 tcttccccag acttttttat cgataaacaa acaggagctg agacaaaaat cttgtctcag
     3061 ctcctttttc cgaataaaaa cggcgttctc ctgccaactc cgcggtaata agtccagcca
     3121 aacgtaacat atgaagagct attttttatt tgactcttct tattacttga tgcagaacag
     3181 cttttttaca acctctgata aacggtgttc aaaaaccagt ttatcggtat gaagtcaaat
     3241 ttatgcaaaa atgtggaaag ccattttcac aaaatttttc ttcatactca aaactaatcg
     3301 gctttttcac aacctcttta tacattattc ctgatccaat atttctttta atgctgcttc
     3361 aagagaagtt aatggttttc ccaatacttt ttctaaatca tcagaggaaa tagctaactg
     3421 atccttttta atatcatgtt ggatcgctaa aaacatgtca gctgcttctt gagggaatcc
     3481 ggcttctttt agattttcga caaacgctgc atcacttgct tcaataattt ctaacttttt
     3541 acctgtcgca cgctctaatg ccttggctaa ttcttcatag tcagttagat tgccggataa
     3601 ttctaagatt tctggagagt cactttctac tagtactttt gctgcagctt ctgcatattc
     3661 acgtttcaat gcccaacctg tttttccatt tcctgcagca taaacgaatt gaccagtttt
     3721 taaagcctgc ccaatgatag gtaattcatt ctctacatac caattattcc gtaaaaatgt
     3781 atgggaaata cctgaatcct taatcatttt ttcagtgaat tggtgatctg gcgctaatac
     3841 actgatcgag tgatcggcat ctgcaaaact tgtataagcg atataagata ccccagctgc
     3901 ttgtgctgcc ttcactacat tcttatgttc ggcttgacga tttcctggaa cgccagagac
     3961 aaaaagcaat cgatctattc ctttcaacgc ttctttcatt gagtctaaat catcataatc
     4021 accaatacgt atattaaatc cagcttcttt cagtgcttct cctttttctt cactacgtac
     4081 tagtacatag atttcatttt gtggaaccat cttttttaag aattccagcg cataaccacc
     4141 aaaacctcca gttgctcctg ttactaaata atgcatattt gccactccct attcttttta
     4201 tttgttaaga tttacagctg tacatttata aattacacct ttatcataaa gagtgtggta
     4261 agataagtca agcaaaaaaa atgaaaggat gacaactgat gaagtattct ctgcaattta
     4321 gtgatgcaat ccatgtactt gcctatattg aaatgtttca agatacccga tcactttcta
     4381 gtgagatgat tgccagtagt atcgaaacaa atgcagcaaa tgtacgtaaa atcatgagtc
     4441 agctcaaaaa atccggtttg atctttacaa aaaccggaaa accgcagcct tcccttgcca
     4501 aaagtccaga tcagatttct ttactagatg tctatcgcag catcgaaggt aatacacagc
     4561 ttatccaagt cgatccaaaa acaaatccaa attgcgtcgt tggtgcgaat atccaagaag
     4621 ctttagaaga aagctacgct cggctgcaga aaaaggcaga agaagaaatg gctaagatca
     4681 cacttgatag tctgatacat aaaatagccg ttttggaaaa agaaaaaaga ccagaaaaca
     4741 caggcctcgt agagagattt ttataattct ctctactgtg cctgcatttt tctgatcgga
     4801 atcgtctaca taaattcttt tattctccgg gggacaaaag ccttgtcatg cccctttttc
     4861 ctctctaatg ctcaggtcaa agcgctgaag tcatgacgtc ttgataaacg gtgttcaagt
     4921 agctgaccct cggcattagt tctgaaaaat gcaaaatatg gaaagctatt tcccattttt
     4981 cctctctaat gctcaggtca aagcgctact ttcacgacct ctatattatc atggtttatt
     5041 gactaccggt atccagattt ctcccatata tttcccgttg gtaactccca tttcgactac
     5101 agcatttgga cccccaacat aggcgtaatc ttctacttct cttaaaacat gcccaaaggc
     5161 cgtattcgtc aaatcatttc ttaattcttc aggcgattct gctaaagcga ttacaacaag
     5221 atacgttcct tttgggaatg ggatgattcg ggtagtgttt ggaacatcct gactcgtttg
     5281 aacaccgaca tagtacatca ttttcccgtt atagggttcg ttgactgtaa agaaataatg
     5341 attttcagca gcttgtttca atgcatctat tgttccatta ccgataactt tttgctcaaa
     5401 tgcttgtttc tctgcagcca aaccgacaaa atccgtattg ctactcttca attccacacc
     5461 aatacccgtc accgtaaaat ctggtttttc ttcgatccta tactttgcca tttctcccac
     5521 cctttctatt atattaagtt catcttacat aaaattaaag ggaaatgaca cgattacgct
     5581 cttttttcta gggatatttt tattttttaa ctattttttt gctttcactg caatccaaat
     5641 ctcactgtaa acattactga aatcctctcc ccattttgta aaagagatct cgggagcttc
     5701 gactaactca taatctgaag aaggaagcca ttctgcatag attttccccc aagtatcttg
     5761 taaagtttgg ggaaatggcc ctttattagg aaaaatggcc caagtgtgtt cttctatagc
     5821 aagcatttct aaatcagcgt acggattttc ttttgtcgta gcaaagccaa tcatgtgcat
     5881 caaactgccc ttttcttcca agcgggctcc atcaaatcca taagaaacgt tgattacttg
     5941 atttggatat aaatctccta acttatgcat ctcttccctt tgagtctccg tgatcgtctg
     6001 cgctaacttt tgtatttctt gattgactcc ttcaaatttg acaggaactt ttttcgccac
     6061 acctgccaca taaaactgtt ctttcttttc gatacgaaat tccatcgatt ttcctcctct
     6121 gacatcaata aaaaatgtca agcgtggaaa agatttttgt atgccgtatc tgctgatttc
     6181 tgaaggcaga tatcctgacc aatcacgaaa agctcttgaa aaaccttcga ctgtttcata
     6241 tccatatcta aacgccgttt ctgtcactct tgatccatct aggagatcgc gattagccaa
     6301 agccaatcta cggtttttga tatagtccat tagcgacatc cctgcaataa acgtaaagat
     6361 gcgttttaat tgatattcag agattcctgt attttcggct attattttat aggaaagtcc
     6421 ttctgtcaaa tgttcttcga tgtaatcgat caattggttc agatctttaa gcatatttat
     6481 cttcctcctt gcttgccttt ttatcatatt atcaaagaaa gtcaattact tgatgaatca
     6541 ggaaccaatt tagtcagatg tagaaaatgt tgtatggatc taaagaaaat tctcaagcag
     6601 cgtttcgtaa cggataattg gtattatctg cctatctatg ttttaccttt agaaaaataa
     6661 attttgggga agacacattc taagtaaaca aagcggtaaa aatatctttt attcccttag
     6721 ttaatccgct tactacctac tgtacaaagc aaattgttgt tcaaactgct gatcagaacg
     6781 ctgcttggat ttttctttgc gatacatcat acctctttta tcaattggaa caattattta
     6841 tttctaacaa aatcacttca tttttgtcag ataaataaaa aacaaaaaaa gttccatctt
     6901 taaaacacgc tattctttta ctttttctca cattctctaa gaaaagaaaa ggagaatgaa
     6961 tttttgacaa ttcgagcaaa agtgattatc aaatatgtaa aatcagctac ttttttctta
     7021 aacgtttctt tatactaaat ataaataagt gtttcatgaa aggagaacta gatgaataat
     7081 tggcttgagt attttcctga aaacgttttg gaacgcggtt acagctatca tctgcacggt
     7141 tttgtccgtc atttgaacta tacaagcaag tacttgtccg caacagtttc tggcacggaa
     7201 gattataagg tagtgatcac ttgggatgaa aagaccaaca tgacttgtga ttgtctatat
     7261 gccatagaag gaaaaaaatg caaacacatg gctgctgtct tattcgctta cgaagaacgt
     7321 ccaataaaaa aatccaacta ctcactttcc gaactctcgt cccttgtttc ctccgctagt
     7381 tcctctcttg tacgggagct tttaacagag attcttatag aacatccgca atttatcgaa
     7441 cgcttcaaag ttaagatgcc atttcatgca ataaattatt cagataaatt aacaactatc
     7501 atccataaat acgatcatat tatcaagaaa aacaaaaaca ggaaaacagc gaagttcatc
     7561 atggagatgc gaaagttcat ccaagaagca gttgaatcgc tcattcagca aaatgcctat
     7621 cttcccgcat tcgaattgat caatgaagtg attgctactc tcgaaacatt ctattgggag
     7681 cctgaggatg aacgaaccct tcttctgata gaggactgtt attatttatg gaaagaatta
     7741 ttagcagaag cacctcatgc agaaaaaagg caaatgttct cttggttcgt ctgccaagtc
     7801 gatcatactg acgcctctta ttcaaaacgc tactccataa aaatcctgaa agaagacttt
     7861 cgggaaaaag aattttctaa ccaaaagaaa aaaatagata aaaaaactaa agaaacggta
     7921 aagaaagacg atttcaatga aaaatagcaa gtaaacgata acaaccatcc taaatccaca
     7981 agaaatacag cagaccttgc tttaaaaaca cgaatcatgc aaaggatgaa gtaaagaaga
     8041 cttaacgcgt acccaatatt agtcatttga ctattaggta cgcgttttgt tttataagtc
     8101 atgacaaatt tccttcgttg ctgggtagat tttccggcat tgttgataga ttttatgata
     8161 cttttctaca ttttgtaaga gaggttccac tgcatctgta tattggataa aattctgaca
     8221 acaactttca ggtgtgtcga accactctaa tctttaaaga
//
LOCUS       DAJSKS010000118         8178 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJSKS010000118 DAJSKS010000000
VERSION     DAJSKS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.120"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            186..962
                     /locus_tag="ON396_002242"
     CDS             186..962
                     /locus_tag="ON396_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HCR3114304.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            977..1180
                     /locus_tag="ON396_002243"
     CDS             977..1180
                     /locus_tag="ON396_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114305.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            1196..1534
                     /locus_tag="ON396_002244"
     CDS             1196..1534
                     /locus_tag="ON396_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCR3114306.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            1521..1700
                     /locus_tag="ON396_002245"
     CDS             1521..1700
                     /locus_tag="ON396_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114307.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(1743..2213)
                     /locus_tag="ON396_002246"
     CDS             complement(1743..2213)
                     /locus_tag="ON396_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114308.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            2300..2998
                     /locus_tag="ON396_002247"
     CDS             2300..2998
                     /locus_tag="ON396_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HCR3114309.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            3176..3517
                     /locus_tag="ON396_002248"
     CDS             3176..3517
                     /locus_tag="ON396_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114310.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            3510..4181
                     /locus_tag="ON396_002249"
     CDS             3510..4181
                     /locus_tag="ON396_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCR3114311.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            4187..4879
                     /locus_tag="ON396_002250"
     CDS             4187..4879
                     /locus_tag="ON396_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114312.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            4876..5625
                     /locus_tag="ON396_002251"
     CDS             4876..5625
                     /locus_tag="ON396_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114313.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            5637..5906
                     /locus_tag="ON396_002252"
     CDS             5637..5906
                     /locus_tag="ON396_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114314.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            5912..6076
                     /locus_tag="ON396_002253"
     CDS             5912..6076
                     /locus_tag="ON396_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114315.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            6069..6368
                     /locus_tag="ON396_002254"
     CDS             6069..6368
                     /locus_tag="ON396_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114316.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            6368..6670
                     /locus_tag="ON396_002255"
     CDS             6368..6670
                     /locus_tag="ON396_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HCR3114317.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            6710..7075
                     /locus_tag="ON396_002256"
     CDS             6710..7075
                     /locus_tag="ON396_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114318.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            7565..7705
                     /locus_tag="ON396_002257"
     CDS             7565..7705
                     /locus_tag="ON396_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114319.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            7702..7884
                     /locus_tag="ON396_002258"
     CDS             7702..7884
                     /locus_tag="ON396_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114320.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            7897..8091
                     /locus_tag="ON396_002259"
     CDS             7897..8091
                     /locus_tag="ON396_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114321.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
BASE COUNT         3031 a         1256 c         1691 g         2200 t
ORIGIN      
        1 ctaaaaagac aacaaaaaac actattgata tgtaaatgca gaccgccaag aaagctttaa
       61 aatcaatgtt tttatgtctt atttaattat gctcttagta tagagtatta ctcagatact
      121 gtcaattgaa tttagaaaaa agttgttaaa tttgtttacg aatataaaag aaaggaatga
      181 gaaatatgaa cacaccgcaa atttttaatt tcgaacaaaa cgaagttcgg acaattttag
      241 taaatgatga accatatttt gtaggcaaag acgttgcgag tgttttgggt tattcaaaca
      301 ctaaagatgc tttgtcgcgg catgtagatt tggaagataa gatggggtcg cgaatcacga
      361 cctcaggtca atcaagagag atgacaatca tcaacgaatc tggtttgtac agtttaatct
      421 taaaatcaaa acttccctct gccaaaaaat ttaaacgttg ggtaacaagt gaagtgttgc
      481 cagcaattag aaaacatgga ggttatctaa ctccagaaaa agtagaagaa gctttgctta
      541 atccagatac aatcattcaa ttagcaactc aactaaaaga agaaagaact ggaagattaa
      601 tcgcagaaca aaagattgca gagtacgaac ctaaaatctc ctatttagat agcatattat
      661 cttctacaga ttcagtaaca attagtcaga ttgcagcaga ttatgggatg tctccacaac
      721 agatgaataa attacttcat aaactaggta ttcagaaaaa agtcggtaac caatggttat
      781 tgtgcaaaaa acacatgaac caaggataca caaaatctca tacaactgag atcccgaaag
      841 ccgatggtgg cactaaaatt gtaatgaata ccaaatggac acagaaaggg cgtctattta
      901 tctacgaatt actaaaaaaa gaaggatatt accctcaaat ggatttagag gaaattggtt
      961 agaaaggagt tttagtatga ctgacattgc agaaatcact caacgagata gagaaaaaat
     1021 caaagaatat gtcgaaagtt caaagttctt aacttacacc atgcttgctg aaagatttgg
     1081 aattagcaaa agctacttat ctttaatttt aaacggtaaa aagacttctg cagaagcaaa
     1141 cagaattata gattcgatta tcactatgta cgaattgtaa agggaggaac agctaatgca
     1201 atatctagaa gcaaaaatcc caattccaga aggctatgta attatctccc aagtggatta
     1261 tgaggagtta aaaaaagctg atgatactgg tagatggatg acgttgccag aagtgctaga
     1321 acggattaac agaaaatatg attggtttac ttctagagtt ttaaagaacc caagatatag
     1381 aaatattatc gatatcgaga aaaacaaaaa tggatttgtc tattatccag ttgaaggaag
     1441 agacacatat ctatttttaa gaagtaaaac acttgaattt ttagaaacaa atttttcgga
     1501 aatcttaagg aggcaagcgg atgggaaaat ttaatagagc gctagtgttc agcgcaccgc
     1561 taatcatcta cgctttagga ctttggggaa gcaggcaagc gttgatagga acgattgttt
     1621 acatggtttg gatttttatg gggcttgatg aagctgaagc tgagtacaga gcgaaaaagc
     1681 caaccgaggg ggctgactaa aaatgaatag aaaagagaaa ctagaatgta tattactatt
     1741 actcagttta attctttcac taatttctct attgggaagt ttgtattttt gatatcaaca
     1801 attttgtgga tttgggttct atttagagta aatatcattt ttgtttggtg attaaggagt
     1861 ttattaaaat taattttccc tacttcaaat gcaaggtaac aagatatagc acttttagga
     1921 ggaatattta gcggtacagc ttcagagtat attctattat catcgactga tcctccttta
     1981 gcaattaatt tcttaaatgg agtagctgac catctgcggt ttaagttagc ggatgaaagt
     2041 tctaaattaa ctaacatagc tggttctgtt gaatagttag aaatgattac ttttgtataa
     2101 atcatatctg cagcgaagta agaagcatta agttcaactt ctagttgagg gcgcattcct
     2161 tttattttta tccctgttaa aaacgtactc aatataaaac ttattattga catccattga
     2221 aaaattgtta agtgaaaaaa attcaaaata aacaccacca gtttttaact aaattatacc
     2281 aaaaaggaga gaagaaataa tgcaagaatt agtaattttg aaaaataaag aagctgtgac
     2341 tacgagctta caagtggcag aaagcttcga gaaaaaacat caacatgttt taagagatat
     2401 tgatgcacta aaaaaagatg tgtccaattt tggacagatg tttgtagaag gtaatgaacc
     2461 agattcatat ggcagaaatc gacgagtttt cttcattagt agagatggat ttttcttgct
     2521 ggctatgggg tttacaggaa agaaagctat ctacttcaaa caaaaataca ttgaagcatt
     2581 caacgaaatg gaagatgtta ttcgcaagaa tactgttcct caaacaattg aagatatgat
     2641 gatctatcaa ttagaagaaa tgaaagatgt taaaaaagat gtttccatgc ttaaagatac
     2701 tatgcgaatt agcggacaac aagagtttga aattaagcaa aaaggaaata tgaaagttat
     2761 gggagttcta gggggaaaag aaagccgagc ttatgaagaa atcagcaaaa aagtattctc
     2821 aaaattttgg tctgaattta aacgtacctt ttcaatccca agatatggcg agttacctcg
     2881 taagagattc gatgatgctg tttcatttat tgaaatgtgg ttgccagaaa ctgcgatccg
     2941 tatggaaatt gatcaactga acagacaaca gagacttttt ggtgatgaca atgaatagag
     3001 ctgaagcgct aagaataggg acggcaattg ctaattgctg gtggaaatac tataaaccaa
     3061 tcatcctaag ccaacaacat attgacaagc aaaaagcatg gcaacaaata aaaaagtgac
     3121 tccgccggca agcaaagagt cacaaagaaa acacatcata aggagatttt agcatatgga
     3181 aaaagaactt tccactctag atcaatattt gattgatcct gattggggca agccgaaaat
     3241 tgaggaaaca agtggtcgaa aaatcagacg aaatcttttg acgaatgaag aactagcttg
     3301 ggatcaagat gatttaggca accatgtaac tatttgggat catgtttatc ttatccatct
     3361 atcgaagcat tcgaataaac ctgaatatat ttacgtcatc gaagatggct tgattgatgc
     3421 gctagaagag tacgacagag ataacttgat tgatatctct tattacggac caggtaagaa
     3481 atacattgct gaaatggagg cagaatttga tgagtgaaat caaagggaca acgaactttg
     3541 aaaaactttt tagtcgtaag ttaaataaaa ttctcaagaa aaaaggaaat tttgattatt
     3601 tatcttgggc tcacgcgtgg gagattatga aaaagaatga tccacaggca acggtaacta
     3661 ttaatgagta caaacactac agggttgttt ctggaactca tcaagacttt cttgttgagg
     3721 aatataaacc ttttcttatg gatgaaactg ggacttatgt atctgtctca gtaacggtta
     3781 aaggacacac ggaaaccgaa ttatttcctg ttttagatta tcgaaaccaa ccagttgtta
     3841 aaccaaatgc aatgcaaatc aataactcat tgaagcgatg ctttgtgaaa gcattggctc
     3901 tacacggact gggattatat gtatttcaag gggaagatat tccaacacca cctagaatcg
     3961 atacaaagaa attaaacatg ctagagacga ttctagaagc tttcaatgag cagatgggta
     4021 aagatatgac caaaacctta atcgaatatg ttaatgagca gacagataaa ttagggctct
     4081 tagctgataa cgttgaaact attgaacagt taagctatga gcaatgtgcc ttgatggagc
     4141 gagcaatagc agctaagaga aaagaattag ataagaagtg atatgagtgt ttaaaccatt
     4201 aatcgattca tattcagcgg ttctgaaaaa gttcaaagga aaagacataa gcgcaaccat
     4261 caatgaggaa gtgaacattg atcgactaaa gacgatgtat gacggctacg atggtgatcg
     4321 agtcattgaa attcgtttta ttgatcctag acgtttcacc gtacagcaac gaaacttcat
     4381 ctatgcgctg ataggcgata tttttatcga tacaggcatg ccaacggact tctggaagga
     4441 attcttctac tttcgttttg aaggtgtcac agggcgcaaa ataagcctga aagacgaatc
     4501 gaatacaact gtgagtgatg ccaatgtctt agcaaatatc atcttagatt tcatctttga
     4561 acatcatatt cctttcaaag aaggctatga gattttacct gcgaatcaag aatattactt
     4621 ctacaaatgc atcacaaaaa gagtctgctg catctgtggc aaaacaggag ctgacatcga
     4681 tcactttgac aaagcgctag gaagacgaaa gcgcaaagaa gttgatcatg cagagtacac
     4741 atttgcagca ctctgcagaa tccatcacac agagaagcac aaaataggtg tgattaattt
     4801 caaaaataaa tatcaaatca aagggatcaa attaaaccag aaaacaatca aaaagttaaa
     4861 tattggaggg gaaaaatgac agaacatcga agttattatg cgattatacc agccaacgta
     4921 aggtacgaca aaagacttaa accaaatact aagttgttat acggagagat aacggccttg
     4981 tgtaatgaaa gaggcttttg ttgggcaggc aatgagtact ttgcagattt atatggtgtg
     5041 aataaagaga ccatatcgcg atgggtaagt gatttgatta agtttggata cttgaatcgg
     5101 gaaatcattt acaaagaggg taccaatcaa ataatcaata ggtacctacg aattaatcaa
     5161 taccctattg acgaaaaacg caatacccct attgacgaaa aagtcaaaga taataataca
     5221 tctattaata atacatttaa taatacaaaa gaatatataa gagagttacc gccttcgaaa
     5281 aaatcgaagg ctaagcccgt ccgtcataaa tacggagagt ataaaaatgt tcttttgtca
     5341 gatgagcaga tggagaaact caaaacagaa ttccctaatg attaccaaga gcgaatcgaa
     5401 cgactgtcag agtattgtga atcatctggt aagacttata aaaactattt ggcaactatt
     5461 cgaagttggg caaggaaaga aaaaagtgaa cctaagaacg caagcagtgg atacaagcgc
     5521 acaggaagac gagagaagct tcctgaatgg gcaatcgacc aagaagccta tctcaagaaa
     5581 aaagcgctag aacgagctaa tagacaatca aaagcaccat tctaagaggt ggaaaaatga
     5641 agatcgatta tctagaacta attaatgaaa tagcgaatta taaaaagggc gaggaattag
     5701 acgtcctgag agacgtatat gatcaactcg aagaagctgg aattgaagga attaagaatg
     5761 atcgttcgag ttggagtaaa ctcagatact atttcgcact ctatatcgat ggaacgcaat
     5821 taagaaattt agcatatacc aaattactat tcattgattg tgttaaaggc ttgcaaaaac
     5881 atcttaatga acttgagcag gtgtaaacaa gatggaccta aagacattta cagcacagat
     5941 cgaattaatg catcaagaag ctttaagaca aagtgtgtcg tacgaagaca agtggctcaa
     6001 cacatttcat ggcggacgtg agagcgcact tgatcaagta ctcaaattac tgaaaggaga
     6061 atgtcgggat ggataagaaa gcggcaatgc agcgaattat cgaattgact tattcagaag
     6121 attggcagga agataaaaaa atagttgcag aagtccaaaa gctcggtaaa tcaatgtgga
     6181 ctgaaaagcc caaacggaga acgccgagaa agattgcaat ctggcatggt gatcgaattc
     6241 tagtaacggg taccgctaaa cagttatctg aaattactgg tctgagcaaa aatattattt
     6301 gggatagagc taggagctta tggattgatt caaaaggaag acagtttagg tatgtggagg
     6361 agaaataatg gacgaactaa tcgcgaaagt agagcagtgg gcaaaagata agggattgga
     6421 tcaagcggat ttcagcaagc aaatgttaaa aacggtcgaa gaaatcgggg aagttgccgc
     6481 ttctctagct agaaaagatg aacatggttt aagagacgga attggcgatg tagtagtaac
     6541 cttgatcatc ttggcaatgc aaaatgatat ggatttatac gagtgcttgg attttgctta
     6601 tgacgaaatc aaaggtcgga ctggaaagat ggtcaatggt gttttcgtta agtccagtga
     6661 cctgaaataa ttccattaat ggaagaaata actgaatagg aggatagaca tgattgatgc
     6721 aaaacaagtg caaaaacaga aagatggaat gttgatgttt gaagcatatg ttctaccatt
     6781 tcttaatcaa tttgaagttt tggaatgctc tgccagcgga gaagaattgg agtatgttgt
     6841 aattcgtgaa accaaagaaa acgttcagaa gctaaacgaa tttctttgca ctattaactg
     6901 ttgggatatg atagctccag gatttttatg tccagctatg ggagaatttt tggaatattg
     6961 tcgtcttgaa gatgcaggca ctttggattt agcttatctg gtatacaact acctaaacat
     7021 caacacagat catttgtggt ttggcactgc cgaaagaaaa tgggtagtcc gctaaccacc
     7081 aatatcaatg aaaaaaggcg gtttcctgaa agtggaattg ccgccattgc tgtaaggttc
     7141 tttacgcgac tcaaggtcac agaaatataa atgcaaatct cttttgaagt gatccaaagc
     7201 tctcggaaaa tggaagtttt ttgtttcaaa gaataaatga gatttgcgtc aagaacctga
     7261 gagttatttc caaaaaagct tccaaagaac cgaatgagct aaggacatca tgtgtaaaca
     7321 cataatatag ctctcctttc acggctaatc ataacatgaa aaatattcga tgtcagtagg
     7381 aaaaaaggtg aattatgaaa agaatattaa attatcccgg cagtaacaga ttctgcaatt
     7441 aaactccgaa gctgatgtat cagtatcaac tgtagaatat tatgagcgtg gagaaagtca
     7501 agcagtcaca aatgtaaaaa tcgataacgt caacggtctt atctgtttag atgaaggcga
     7561 tagtatgaat gaaattgctg cagaccatga attatcagaa actgctttaa ttgatcaaat
     7621 cgaattagct agacaatact ttgttccata tgcagatacc tggtccaagc ataagaatga
     7681 catcattttt ccagaggaac aatgagagca actgatccag taattatcct tgaggaagcc
     7741 aaatttattt ggactcacga agagatagag caagcacgct tgctcttttc tcaaggagtt
     7801 aagccgagca aagtagctga aataatgggt caaaagattc ttgatgtcgg attgcttttg
     7861 ctccatctag cagaaaaaat ttgatttgag gtgaaaatga tgattcaact tgcaggcatc
     7921 caaacaggga aaatttattt ttctggagaa agcaaaagcg aagccaacca atggctattg
     7981 aaaacttaca cgaacaataa gaagctaagg aaaaaatatc ctgatttgtt gctaaaggat
     8041 gatcagatta tgccggaacc gatgattttg acccgtaagg agggaagttg aaactaaaaa
     8101 accagccgtg tgggctggag ttgctatttc tttttaataa tgagtttttc taaatttaat
     8161 tcattctcac gagtttgc
//
LOCUS       DAJSKS010000119         8070 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJSKS010000119 DAJSKS010000000
VERSION     DAJSKS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.122"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(133..306)
                     /locus_tag="ON396_002260"
     CDS             complement(133..306)
                     /locus_tag="ON396_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3114322.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="ON396_002261"
                     /pseudo
     CDS             754..3116
                     /locus_tag="ON396_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="ON396_002262"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="ON396_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR3114323.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="ON396_002263"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="ON396_002263"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCR3114324.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="ON396_002264"
     CDS             5704..6489
                     /locus_tag="ON396_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3114325.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="ON396_002265"
     CDS             6502..7353
                     /locus_tag="ON396_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HCR3114326.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="ON396_002266"
     CDS             complement(7477..7929)
                     /locus_tag="ON396_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114327.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         2714 a         1373 c         1711 g         2272 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg
//
LOCUS       DAJSKS010000120         8055 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJSKS010000120 DAJSKS010000000
VERSION     DAJSKS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.123"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..507
                     /locus_tag="ON396_002267"
     CDS             <1..507
                     /locus_tag="ON396_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A chloramphenicol O-acetyltransferase"
                     /protein_id="HCR3114328.1"
                     /translation="EIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFN
                     KQTEKFTNIWTESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDF
                     SSFNLNIGNNSSFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDI
                     IHRVDDWI"
     gene            complement(1200..2138)
                     /locus_tag="ON396_002268"
                     /pseudo
     CDS             complement(1200..2138)
                     /locus_tag="ON396_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(2138..2755)
                     /locus_tag="ON396_002269"
     CDS             complement(2138..2755)
                     /locus_tag="ON396_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3114329.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(2769..2939)
                     /locus_tag="ON396_002270"
     CDS             complement(2769..2939)
                     /locus_tag="ON396_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114330.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(3287..4777)
                     /locus_tag="ON396_002271"
     CDS             complement(3287..4777)
                     /locus_tag="ON396_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HCR3114331.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(4820..5119)
                     /locus_tag="ON396_002272"
     CDS             complement(4820..5119)
                     /locus_tag="ON396_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114332.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            complement(5485..5769)
                     /locus_tag="ON396_002273"
     CDS             complement(5485..5769)
                     /locus_tag="ON396_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3114333.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(6288..6773)
                     /gene="vanZ"
                     /locus_tag="ON396_002274"
     CDS             complement(6288..6773)
                     /gene="vanZ"
                     /locus_tag="ON396_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HCR3114334.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(6926..7837)
                     /locus_tag="ON396_002275"
     CDS             complement(6926..7837)
                     /locus_tag="ON396_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HCR3114335.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         2430 a         1456 c         1203 g         2966 t
ORIGIN      
        1 gaaatttatc cctctttaat ttatgcaatt atggaagttg taaataaaaa taaagtgttt
       61 agaacaggaa ttaatagtga gaataaatta ggttattggg ataagttaaa tcctttgtat
      121 acagttttta ataagcaaac tgaaaaattt actaacattt ggactgaatc tgataaaaac
      181 ttcatttctt tttataataa ttataaaaat gacttgcttg aatataaaga taaagaagaa
      241 atgtttccta aaaaaccgat acctgaaaac accataccga tttcaatgat tccttggatt
      301 gattttagtt catttaattt aaatattggt aacaatagca gctttttatt gcctattatt
      361 acgataggta aattttatag tgagaataat aaaatttata taccagttgc tctgcaactt
      421 catcattctg tatgtgatgg ttaccatgct tcactattta tgaatgaatt tcaagatata
      481 attcataggg tagatgattg gatttagttt ttagattttg aaagtgaatt taattttata
      541 cacgtaagtg atcataaaat ttatgaacgt ataacaacca cattttttgg ttgcttgtgg
      601 ttttgatttt gaatttggtt ttgaacttat ggactgattt attcagtcca ttttttgtgc
      661 ttgcacaaaa actagcctcg cagagcacac gcattaatga cttatgaaac gtagtaaata
      721 agtctagtgt gttatacttt acttggaaga tgcaccgaat aaaaaatatt gaagaacaac
      781 tagcaaaaga ttttaaagag ttattttatt ttaagtcttt ataacatgag tgaagcgaat
      841 ttttaaattt cgatagaaat ttttacatca aaaagccccc tgtcaaaatt gacgaagggg
      901 gttttttggc gcacgctttt cgttagaaat atacaagatt gaaaatcgtg tataagtgcg
      961 ccctttgttt tgaacttagc acgttacatc aattttttaa aatgatgtat aagtgcgccc
     1021 ttttaaattt tgagtgatta tattttttga gttagaaaaa gggattggga aaatttccca
     1081 aaataattta aaaaataagc aaaaattttc gatagagaat gtgctatttt ttgtcaaagg
     1141 tgtatacctt gactgtgctt gctgttacat taagtttatt tttaagttat taaaaaagaa
     1201 atagctttta aagtttggct cgctgtcgct ttataaagct gattgacttt tgattgcaaa
     1261 ctacttaaag aaaacaaact cggactattc gttttcttct ctttggtttg aacatcagca
     1321 attatcccct cttgattgcc tattttagct tgtttagaag aaacaaaagc taaaagctcc
     1381 tcttgggttt taaaacgctg tgtggggctt agaacgccct taaacgaccc ttggtttact
     1441 tttatactag cttccacctc gaaaaaaggt tcttttttaa aattctctat ggcttcctgg
     1501 cgctgaaaaa taaggtataa ggtgggcgtt tgaacacgtc ctagtgaaaa tgtaccttgt
     1561 acgcccttct gttgtaaatt taacgtatac aaagggcttg cgttcatgcc gatcaaccaa
     1621 tcggcaattt ggcgtgtttg cgcttcttga taaaagggat agtaattcat tccaggttgc
     1681 aaattttgaa aaccgcttcg gattacatct ttttctaagc tattgatcca tagtctttta
     1741 aatgttttat cttttgaaaa ggcatttgct ttatggataa tcgaccaggc gatattttca
     1801 ccttctctgt cgctatctgt tgcaacaata attgtatttg cctttttgag aagttctgca
     1861 acaattttaa actgctttcc cttatctttt gcaacttcaa aatcgtatcg atcaggaaaa
     1921 atcggcaaag attcaagttt ccaattttgc cacttttcgt cataatgacc tggttctgct
     1981 aattccacta aatgcccaaa accaaaggtg ataaacgttt catctgtaaa tagtgggtct
     2041 ttgatctcaa aataaccgtc ttttttggtg ctttgtttta aagcacttgc gtaggctaat
     2101 gcctggcttg gtttttcagc taaaataacc gtactcatta actatccctc ttttcattgt
     2161 tttttctttg atcgactgtc acgttatatc ttgctcgata ccttctaaac gttcggcgat
     2221 tgattccagt ttgttcttca acttctttat cggataaacc attcaaaaac aaatcgaaag
     2281 catgctttaa acgtggatca ttttctttaa atttatgctg acgacctttg aatttgcctt
     2341 ttttcttagc aatttcgatt ccttgtgcct gacgttcctt aatttttttt cgttctgatt
     2401 ctgcttgata cttgtacaat tcaatgacaa ggctattaat caaacgcctt aaattttcat
     2461 cttcaatacc attcattgag ggtaaattta agacttccag ggttgccccc ttaatttgaa
     2521 tttgattcat caattctgtt aattctttat tatttcgtcc taatcgatct aattcagtaa
     2581 caataacaat atccccttca cgaatatagt taagcatagc ttgtaattgt gggcgttcga
     2641 ccgattgacc gcttaatttg tctgaaaaga ccttagaaac gccctgtaac gcttgtaatt
     2701 gccgatctaa gttctgttct ttgctactga cacgtgcata accaatttta gccattttca
     2761 accaacctct aaaattctct cggttgcaat aaccaatcag caatatctac tttttcaatt
     2821 tcaaattgct tatcagaaat tgtcttttcg taagcgataa aatcttgcgc atattgttgc
     2881 tcattaaaaa tagccaccac ttcgtcattt tctaaaactc gataaataaa ttttttcatt
     2941 ttactcctcc tattatgccc aacttaaatg acctattcac caagtcaatt atactgctaa
     3001 aatcatatta ggacaaatag gtatactcta atgacctata aaagatagaa aattaaaaaa
     3061 tcaagtgttc gcttcgctct cactgcccct cgacgtttta gtagcctttc cctcacttcg
     3121 ttcagtccaa gccaactaaa agttttcggg ctactctctc cttctccccc taataattaa
     3181 ttaaaatctt actctgtata tttctgctaa tcattcgcta aacagcaaag aaaaaacaaa
     3241 cacgtatcat agatataaat gtaatggcat agtgcgggtt ttattttcag cctgtatcat
     3301 agctaaacaa atcgagttgt gtgtccgttt tagggcgttc tgctagcttg tttaaagtct
     3361 cttgaatgaa tgtatgctct aagtcaaaag aatttgtcag cgcctttata tagctttctt
     3421 tttcttcttt ttttacttta atgatcgata gcaacaatga tttaacacta gcaagttgaa
     3481 tgccaccatt tcttcctggt ttaatcttaa agaaaatttc ctgattcgcc ttcagtacct
     3541 tcagcaattt atctaatgtc cgttcaggaa tgcctagcac ttctctaatc tcttttttgg
     3601 tcgtcactaa ataaggcttg tatacatcgc ttttttcgct aatataagcc attaaatctt
     3661 ctttccattc tgacaaatga acacgttgac gttcgcttct ttttttcttg aatttaaacc
     3721 acccttgacg gacaaataaa tctttactgg ttaaatcact tgatacccaa gctttgcaaa
     3781 gaatggtaat gtattcccta ttagcccctt gatagttttc tgaataggca cttctaacaa
     3841 ttttgattac ttctttttct tctaagggtt gatctaatcg attattaaac tcaaacatat
     3901 tatattcgca cgtttcgatt gaatagcctg aactaaagta ggctaaagag agggtaaaca
     3961 tgacgttatt acgccctatt aaaccctttt ctcctgaaaa tttcgtttcg tgcaataaga
     4021 gattaaacca gggttcatct acttgttttt tgccttctgt accgcttaaa accgttagac
     4081 ttgaacgagt aaagccctta ttatctgttt gtttgaaaga ccaatcttgc cattctttga
     4141 aagaataacg gtaattagga tcaaaaaatt ctacattgtc cgttcttggt atgcgagcaa
     4201 taccaaaatg attacacgtt agatcaactg gcaaagactt tccaaaatat tctcggatat
     4261 tttgcgaaat tattttggct gctttgacag atttaaattc tgattttgaa gtcacataga
     4321 ctggcgtttc taaaacaaaa tatgcttgat aacctttatc agatttgata atcatagtag
     4381 gcataaaacc taaatcaata gcggttgtta aaatatcgct tgctgaaata gtttcttttg
     4441 ccgtgtgaat atcaaaatca ataaagaagg tattgatttg tcttaaattg ttttcagaat
     4501 gtcctttcgt gtatgaacgg ttttcgtctg catacgttcc ataacgataa acgttgggtg
     4561 tccaatgtgt aaatgtatct tgattttctt gaatcgcttc ctcggaagtc agaacaacac
     4621 cacgaccgcc aatcatgctt gattttgagc gatacgcaaa aatagcccct ttgcttttac
     4681 ctggcttggt agtgattgag cgaattttac tatttttaaa tttgtacttt aacaagccgt
     4741 catgaagcac agtttctaca acaaaaggga tattcattca gctgttctcc tttcctataa
     4801 atcctataaa ataggttgtt taattaactt ggtttgcttt ttcattcaac tgtttcaata
     4861 ttgcatgttt tgaaaaagat ttttttcctt tataagtcaa tttttttcca ctaatcgaat
     4921 aaattatttt gttattttct attaacttat atatataatc ttccccctcc gaagaaaaat
     4981 acttatctga ttttgtttct aagtagatat ttctcttttc taactctttc ttaaacgttt
     5041 ctagtgtata gatatttgct aattttctta tctccaataa actatttttt atataagttt
     5101 tacattcatc atgattcata caaactccac cttctataaa tgaatacaaa aaaagcaatc
     5161 aaacgatttc cgattgattg cttaacaatt cttaaattca gtagcttaga tacttgaaaa
     5221 ctctctgatt tccctatata atgatagtac ggttatatac cgtcttcaaa caaagttaat
     5281 taaataactt cttacgaggg aagagttcat ctgactaact gataagcgtt ggtttggcaa
     5341 tcttatcggg ctatgcattt ataaaatgtc gtcaaacatt ttataaatgt gtcatggctc
     5401 ttttttcgtt tctattcagt tcgttgtttc gttatatcta gtataccgct tttaaaaaaa
     5461 ataagcaacg atttcgtgca ttattcacac gaagtcattg cttttttctt cttccatttc
     5521 taaatccaat gttacttgtt ctgattctgt ttctggttct ggttctgttg gctcatttgg
     5581 gattaaatcc actactagcg ttgagttagt taactttgca atttgttcta gtgtttttat
     5641 ggttggatct gattttcctg attctattcg tgaataattt gatctactca tttctaattc
     5701 ttgggagaat ttttcttttg atttggtacc tctcatcttt tttaagcttt ctctaaatgc
     5761 tagttccaag tttctccccc ctctttttgt gtaataaaat tcacgcttta tttaaattat
     5821 agcttttaat atcttaataa acaagtgatt aatcacttgt gatttgtgat tattccaaaa
     5881 gtaggtaatt tcaccaacct ttttcaaaag tttttatcga cgattattgg actggatcgt
     5941 gtttcctaaa gcgattctaa gcgcatgttt aattttaaag tgggtaattc tctttaaaac
     6001 gtcctacaaa gattcaaaaa aagcatggct gctagtgggg gtaccgtcag gaaaatgcgg
     6061 atttacaacg ttaaggaaat ttattaaaga gaccaattcc tcagcactaa aattgataat
     6121 tgggctttct ttgttactat agtgttaggt aagcttttta ctttatcacc ggttttcccc
     6181 tcacttcaca cctacgggcg agtttcccca catcccgttt tccatctaat ccaattcatt
     6241 caatatcttt atgtgtatga aagatcagat tgcttgattt agacatctta cacgtaattt
     6301 attcttaaat gggtacggta aacgagcaat aatacgagca aaagtatacc tacaaaaata
     6361 ataactctgt ctaatttttt ttgattcata tgcttattgc ttaaaccata taatttcagt
     6421 ccaagaaagc ctccaacagt atttgtaatt acatctgtta tgtctgtcgc tccaatagcg
     6481 aagataaatt gaattatttc aaaagtaaga cttaaaacca gtacaaaagc aaacttaggt
     6541 aaaaatccga tttctttaaa attgacattc aaaagcaagc caaatggaat aaagattata
     6601 acattatcta tcatctctct gaaattccca gtagcagtaa atggagtcaa gttaagactt
     6661 ctttgatgat aattaaatac tgataaaata ttgtattgta atttgaataa cactaaccag
     6721 attagtgtca ctaaatataa agctagcaat cctctagata atatttttcc caatataatt
     6781 tcctctaatt cttttgttta agttgtatca ttagtatagc aatgtcctag ttaacgtggg
     6841 ttttaaatta aaaagctaac gttaccccaa aagtgctagt acaataatgc tgttcattaa
     6901 tttcgttggt ttcattccgc catccttacc tccttgaatt agtatgtgtt gatccgggaa
     6961 acactgtcac aattacaccg tctatattgt ttcctgatat ctcataacga agattagtcg
     7021 gcacatgaat ggtggtattt ttagtaacag gataataaaa gatctcatat ttttccccat
     7081 ttacactaac agaaatggtt ttttcttctt ttaggtaatc catatattcc tcgagaacga
     7141 aattcttttc tttcataatc gcactatgtg gtaaaccaac atagcgaata tgccatggtt
     7201 catattgaat tcctgttaac tctgttttgt cctctggata acgtaaaatg aacccgtatt
     7261 tccaagcatt ttcttctatc cactttcctt caggggctcg ttccattttc gtcaagcttg
     7321 atcctacatc tagtgataaa cctgaattat gctcactata acctgctggt aaggcatact
     7381 cagcccccat ttcttggtaa agcacacttt gctcatcaaa gtctcgatag ccactattaa
     7441 taataaaatg actaacgcca ccctttacag catcattgac catctctgaa aatttttgtg
     7501 ctatttcttt tgacatataa atattactat caagcaaccc gtatccattt attaattcgt
     7561 catgtttaga taaattcacg atatctgact tcacactttc ttggcgaaca ggatatttac
     7621 tattgattaa tagcagattt ccttgataaa cctgttcttc tgtaattgtt ttctcttggg
     7681 tattttcaga agtcccacta ttttctaaat gttctttggg attttgatca taattttgaa
     7741 attcgacttt ttcctgagaa aacagtgctt cattaacgta gtcataacct aagtatatta
     7801 agaataacaa taacaataaa aaaaacaact tcttcatttt cagtctcctc cttaactata
     7861 tagttggaag tatgaaatat ttctagaatc ttccccttaa acggcacaat ctcgtttgag
     7921 attgtgccgt tttgccccgt aaattacagt caattatttc gccctactgg atttactatt
     7981 atcaccaatg tagatgaatt ttcataagtg atacccatgc tgaatttaac gaggctgaac
     8041 tgcctgttga caatg
//
LOCUS       DAJSKS010000121         8029 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJSKS010000121 DAJSKS010000000
VERSION     DAJSKS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.124"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            69..770
                     /locus_tag="ON396_002276"
     CDS             69..770
                     /locus_tag="ON396_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR3114336.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1379..2395)
                     /gene="tsaD"
                     /locus_tag="ON396_002277"
     CDS             complement(1379..2395)
                     /gene="tsaD"
                     /locus_tag="ON396_002277"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR3114337.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2392..2856)
                     /gene="rimI"
                     /locus_tag="ON396_002278"
     CDS             complement(2392..2856)
                     /gene="rimI"
                     /locus_tag="ON396_002278"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR3114338.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2863..3405)
                     /gene="rimI"
                     /locus_tag="ON396_002279"
     CDS             complement(2863..3405)
                     /gene="rimI"
                     /locus_tag="ON396_002279"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR3114339.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3389..4111)
                     /gene="tsaB"
                     /locus_tag="ON396_002280"
     CDS             complement(3389..4111)
                     /gene="tsaB"
                     /locus_tag="ON396_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR3114340.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4302..5282)
                     /locus_tag="ON396_002281"
     CDS             complement(4302..5282)
                     /locus_tag="ON396_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3114341.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5306..6790)
                     /gene="galT"
                     /locus_tag="ON396_002282"
     CDS             complement(5306..6790)
                     /gene="galT"
                     /locus_tag="ON396_002282"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HCR3114342.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6802..7791)
                     /gene="galE"
                     /locus_tag="ON396_002283"
     CDS             complement(6802..7791)
                     /gene="galE"
                     /locus_tag="ON396_002283"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR3114343.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2274 a         1715 c         1411 g         2629 t
ORIGIN      
        1 taaaacttta caaattcttt aaaaaaagtg acaataaata tagtgaacaa taaggagagg
       61 actttttaat gcgtgttaat aaaatcgatt ttcacttttc aaaactcaga tatcaacctg
      121 attttgaaca tttctcggat gaagagttcc aacagatcaa ggaacatact gttcttcgtt
      181 cttataaaaa aggacagatc cttttcgacg atggtgatcc cagacaacgg ttgtatatct
      241 tgaccaaagg actcgttcgt ttagaacgtt atgatgaaag tgcaacttat tactactatg
      301 actatgtgaa tacaaataac ctttttccta tgtcaggaat gtttggagaa gagacctaca
      361 cttatacagc tcaagcgatg actgatattg aaacattcta ccttcctgtt tcctactatg
      421 aaaaattagc acgaaaaaat cccgaacagt tgatctatat gatacaaaaa atttctaaag
      481 tagttgaaat acatgaattg cgtatccaaa tcggattgac ctctagcgcc tttgaccggg
      541 taaaacaaaa tttattcatt ttgaaagaag aattgggcgt accaacaaaa gatggtcata
      601 tcaccattcc ttatccgatc accttaaaag aattagcagt gaatagcggc acgacacgtg
      661 aaactgctgg acaagtaatc aaacaactac gtgattccgg gctgttagat tatgacaaaa
      721 agcgttttac tttctgtaag acagctacta ctccagctat gagagaataa cgcgaacaaa
      781 aataagcagt gaccctgata aatatcaggg tcactgctta ttttcctccc taaaacttct
      841 gaaagatccg gcagttttag atcactggac aagactcaaa gattttctat gtgtgtttca
      901 gttatgtaat attcactgtt tgcaaaaaat actgttagat taactagatg aagcgtcttg
      961 tcctcatccg atctagatta ttcgatattc ctcgacaata aacgattact cagtgagggc
     1021 aatcagagga acggataatg ataaataagc tgtaaaaaat agcaaaataa caataatgat
     1081 taaaagtatg taaaaaatag ggctttgaca aaaacgtgtc acagccctat ttcgattaaa
     1141 cggtgtgtac ctgtcaactc ttcggtcaca agtcataatg ttcataaaca tgaggagctg
     1201 ttttttgaaa ttgttccttg tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta
     1261 aacggcgttc aaaagcttgc atacgcggta ataagaacag ccaggtcaaa aacatgagaa
     1321 gccgtttttc ctgggctgtt cttattactt gatgcagggc agctttttca caacctcttt
     1381 attcagataa aaccaagctt ggatcagcat tcaactgata gctagcaaaa tttctttttt
     1441 gcatttccac atgtgctgct gctccaatca ttgcagcatt atctccacaa agtcgaagag
     1501 ggggaatcac tagttctacc tcagggagtt tagttgccaa agcatcctgc aatccttcac
     1561 gcaacccttg attggccgct acacctccag ctataatcag ttgcttgact ggataatttt
     1621 gacaagctcg aagtgtttta gtaatcaata catcgatcac acttgcttga aagctagctg
     1681 ctaaatcatt tttgtctaga tcttctccac gctgttgtgc attatgcacc aaattgataa
     1741 atgcactttt taatccgcta aaactgaaat cataattatc ttcatggatc atcgctcgcg
     1801 gaaaatggta attatctttt ccctgatgag ctagctgatc gatttctttt ccgcttgggt
     1861 aagaaagacc taatactctt ccaactttgt cataagcttc tcctgctgcg tcatctcttg
     1921 tctccccaat gatttcatat gatccatctt cttgcatata gactaattct gtatgtcctc
     1981 cgctaacaag aagtgccatc aatggaaact ggaatggttt aaccagacgg gctgcataga
     2041 tatgccctgc catatggttt acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct
     2101 tagctgcaga aataccaatc agcaacgagc cgacaagtcc tggtccataa gtgacagcga
     2161 ctgccgataa atcttctgcc gacacaccag cttctaccag tgcatcttcc aagcataagg
     2221 tgatttgttc tacgtgatgg cggctagcta cttcaggtac tacgccacca aaacgtttat
     2281 gactgtttat ttgagaagca acgatgttag ataagatctc tgttccattt ctaacgaccg
     2341 ctacactcgt ttcatcacaa cttgattcaa tcgccagaat caattcttca ttcatttcat
     2401 cgtctccgtt tttaactttc tgctcataat gatcgcatct tccgtcggat tgcgataata
     2461 tgatttccgt ttacctaata tacgaaactt ttctgattca taaagttttc ttgctgcttc
     2521 attagataga cgtacttcta aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag
     2581 taattgtaac aaatgccgtg catgcccttt tccttgttct gaaactgcaa cagcaatatt
     2641 tgtgatctcg acttcatcta ttactttcga gtatccaata aagccacaga tctggttatc
     2701 ttcagctaaa agcagataat cagagtgagg ctgttggatg tctgccaaaa actgcatttt
     2761 cgtccaaggt gaaccatgtg gataagcatt ttcgctaatc atccatacgc attctgccaa
     2821 gtacgcttca tcaaaatctt tttttctgca aatcaactaa tctcactcat tttctaaaag
     2881 ttttatcata tccaacgcat cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt
     2941 tgcttgaaac cccaatttcc gataaagtct ttgtgcatct tgattactca tacgcacttc
     3001 taatgacatc gtctcacagc gattcattat agcaaagttc tcgatttcgt caataagcag
     3061 cgaaccgatt cgtttacgct gatatgccgg tcgaacggca atattcgtga tatggcaatc
     3121 taaaccgaac gctcttgaac cgataaaacc aatcattctt ccattatcaa agatacctag
     3181 atatagatga ggaagcggtg acttcaattc tgatataaag gcactttttg tccatggcaa
     3241 ttcgccatta tagacttctc gttcgacatc cagcagctct ttgatatctt ctgtagtcaa
     3301 ttctcttagt acgtaacgac gatcgatttg ttcgactgtt ttttctgtat aaatcatttt
     3361 atcttgaaaa aagcttctgg catacgctct aaattttttc aacataactt tcgtctccag
     3421 gtgtgtgcgt ctccaaccat ttttcttctg cttctactcg ttttaaatag cgaggaagaa
     3481 aagcttgttt ctctgcaact ggttcaacta aagcacctaa ttgagccaca gttacaccac
     3541 ttggaatatc ccatgctgga actgtattaa tcatcgcact aggcaatatt tcctttattt
     3601 cagagaagaa tttgtgacaa tcactcccga cgaaataaat agcttcattc agcttttgta
     3661 gttgtgtgaa caaatcagaa gcagaaatat gtacatcttc aagtacagtt tctaatctat
     3721 tttctttcca gcggtatgcc ccagcgtata cattttttct tcgagcatca aacatgggca
     3781 caataatatt atccacaccg atgcaattag ccgcgatggt tttcagacta gatactccta
     3841 ctagctcttt atctaatgta taagcaatcg tttttgctgt cgttacacct atacgaagcc
     3901 ctgtataaga accgggacca tcagaaaccg cgattcggtc aatgtctttt ggacttattt
     3961 gaagatctgc aaacaattgg tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga
     4021 tatttgtttg tatttgtcca agaatttgtt cgtcttctac cacaccgatc gccaaagttt
     4081 ggtttgcagt atctattcct aatgtaatca tatatttgat cctttctgct atgacattct
     4141 cgccttccat tgtagcatac ttttttattc tgaggatgaa aataccaaag gcaaaactag
     4201 tacaataata catgattatc tgctggcggg caaattttgc cccttcctga aacacaaaaa
     4261 agctgcggta tcactccatt ttcccacagc ttttccatct tttatttcgt tgattctcga
     4321 ataatcaact ctgtttccac aataatcttt cttgaaaccg gtgctttgga attacttaat
     4381 tgaagaatcg tctccactgc tagctctccc atccattcgg tataaacttt tacagtagaa
     4441 agcgagggcg ttacatattt cgctacactg acatcattga agcccatcac agaaatatct
     4501 tctggtacca ctaaaccttt ttcttgaatc gctttcagtg cgccaatcgc taatgcatca
     4561 ttagctgcaa aaagcgcatc aggaacatgc ggattcgttt ttaaatactg cttcactgct
     4621 tgatatcctg actcgacaga aaaatctgct tcgataattg gaccttcgtc gtagcctttt
     4681 tctgccaaaa cttgttgaaa tgccataatt ctgggatctt ttagccgctg atggtttttc
     4741 ttcgtatatt ctgttccagc aagcatagcg atatgttgcg ctttatggga gacaaagtgt
     4801 tcaatgactt tttgaacact tgacttaaag tcgacaacta gagagtcgat tccttgagaa
     4861 gtgccgtcag aatctaccaa aagcaaggca ttttgggatc ctttcatttc taataactga
     4921 gcttcgtcga attttcctag tgcgatcgtt ccatctgccg gaagatcact aatggaatcc
     4981 cacgtttctt tcaataaagt aacgtttgct tcttctgcct ttttttcgat tcctaggcga
     5041 atagataaat aataaagatc ttcaagctct tccgtatcgt cgtaccactg gatcaggcga
     5101 agcgtttgct tagtatttgc tgcattttta cgatgtttcg tataatttaa gcgttctgct
     5161 gcttcaaaaa ttttttgctt tgtggctaag ccaacggaaa gatcaggatc atagttgaga
     5221 actcttgaaa cagtagcagg agaaactcct gccaactgcg caatgtcttt aattgttgcc
     5281 atttgtctct cctccttttt tacgtttata aaatcgcagt aaatcgttca aacgctgcct
     5341 gtcctttttc atctcgcttg aatactccag cgtcttccag tacacgtgca aaaacctgac
     5401 ctacagattt ttgtacgaca tcggtgaccg tatctttcgt caaatcagga tgttcttttt
     5461 ttaattgttc tgcccacgga cgatgatatt cagccatctg gttttcttct cctaataaga
     5521 aatgctctac ttctactagt tctggtttca aacgaggtgg taaaacagca agccccatta
     5581 cttcgatcaa cccaatgttc tcctttttga tatgttggac atctttatgt ggatgaaaaa
     5641 tcccatcagg atgttcttct gaaacattgt tgtctcgaag gacaaggtcc aactcaaagt
     5701 aattgtccct tcttcgtgca attggcgtaa tcgtatggtg tggtgtacca tctgctgaaa
     5761 aagctctgat agatactgtt tcatccgaat atgctttcca tttttccaat actgagttag
     5821 ctgcttcaat aagcaagttt ttctctgttg attgcaaacg gatcacagac atcggccatt
     5881 tcacgactcc tgcgatgact tgcggataat cttctagttt gataagtttt tctatcggtg
     5941 cttttgccat aggaaattca tgacgtcctg cctgataatg atcatgagaa agaatcgatc
     6001 ctccaacaat tggtaagtca gcattcgaac caacaaagta atgaggcaag acttcagtaa
     6061 tccgaagcag tctagcaaat gtttcctttg tgattttcat cgggcggtgt tgttctgata
     6121 aaatgatcga atgttcattg taataagcat aaggggaata ttggaatccc cagctttcac
     6181 cgtctagatt cattcgaata atccggtgat tggtgcgggc tggatagttc aagcgacctc
     6241 tatatccttc gttttccatg caaagcatgc aggtaggata gtctactttt accgcctcgc
     6301 gttctgctgc gatttgtttt ggatcttttt ctggcttaga taaattgatt gtgatttcca
     6361 gttcaccata ctccgactct gccggaaatt tgatgttttt tgcgatggct cttgttttga
     6421 tgtagtcatt tttcttactt aaattaaaga aatactcagt tgcttcctct gggcttttcg
     6481 aatagtgctg tgcaaagaat gcattgacga ctgaaggtgg cggtgtgaga aaatccatta
     6541 attgcgcttc aaaaatctct tgttctgctg gcaattctga aagaacacca ttttctcttg
     6601 ccttagcgac aagctgatcc aataagtcta cagattcttc cttaactggt cttacttcaa
     6661 tgctttctag agcatcttct cctaccatcc ctaaaagacg attctgtaaa tataagcggt
     6721 ccatttccat ccatccgcca gcttcgatcg caagtgtggc aaaatcacaa atcgtttgac
     6781 tgattgacat atctcattcc cctattcttc ataaccattt ggatggctga catgccaatc
     6841 ccatgctgtt ttgataatcg ctttgatatc tgtgtattca ggtttccagc ccagaatctc
     6901 tctcgctttt tcacttgaag cgactaatcg gctaggatct ccagctcttc ttggtgctat
     6961 ttctgctgga atttcttttc ctgtcacttc tctagctgct tctagcattt ccttgactga
     7021 gtatcctttg ctgctgccta aattgaagaa gttgctttca tttccttctt tcaagtattc
     7081 caaagctaaa atatgtgcag caatcaaatc ttctacttga acataatctc tgatacatgt
     7141 tccatctggt gtatcataat cgtctccata aacagccaag gcttttcttt gaccaagagc
     7201 tacttgtaaa atgattggga caagatgcgt ttctggtgta tgatcttctc cgatagatgc
     7261 atctgctttt gctccggcta cattgaagta gcgcaaagcg acatagcgca tgccatatgc
     7321 ttggtcacac cacttcatca ttttttccat catcaattta ctttcaccat acggattttt
     7381 aggattagct ggtgtatctt ctgtgattgg agactctttc ggctctccat aagttgctgc
     7441 agtagaagag aatacgattt tattcacgtt gaactcgtgc atgacttcaa gtaagatttg
     7501 cataccgtat acgttgttgt tgaaatacat caatggtttt tcgacagatt ctcctactaa
     7561 agagcttgca gcaaaatgga ttactccttc aatcggttcc ttttcaaata cggaacgtag
     7621 aaattcttta tcccggatgt ctccttcata aaaatgagca tctggatgta ctgcttgttt
     7681 gtgacctgtg agcaaattat ctaccacagc tacttgatac cccttttgaa ctagctggtc
     7741 tacagcgtgc gaaccgatat aaccggctcc gcctagtact aaaattgtca taaaaaacac
     7801 tcctttgatt agtaaaattg tgtttgttga ttctatataa tttaaaaaac gaagtttact
     7861 tttatggtga aaacaaacaa agtaaaattt cgtaaattca tatggtaaat ttataataaa
     7921 acatttagta aacattttca aggatttttt gcaaaggttt acaaacaaac gtaaaactag
     7981 cctttttttc tcttttgttc cataatagat ataacaaata ataaatgag
//
LOCUS       DAJSKS010000122         8012 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJSKS010000122 DAJSKS010000000
VERSION     DAJSKS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.125"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            258..2024
                     /locus_tag="ON396_002284"
     CDS             258..2024
                     /locus_tag="ON396_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HCR3114344.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2126..3385
                     /locus_tag="ON396_002285"
     CDS             2126..3385
                     /locus_tag="ON396_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR3114345.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3532..4833
                     /locus_tag="ON396_002286"
     CDS             3532..4833
                     /locus_tag="ON396_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3114346.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4836..5687
                     /locus_tag="ON396_002287"
     CDS             4836..5687
                     /locus_tag="ON396_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3114347.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5700..6515
                     /locus_tag="ON396_002288"
     CDS             5700..6515
                     /locus_tag="ON396_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HCR3114348.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6666..7832
                     /locus_tag="ON396_002289"
     CDS             6666..7832
                     /locus_tag="ON396_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3114349.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         2615 a         1379 c         1592 g         2426 t
ORIGIN      
        1 tctacgccaa aaactgttaa aaaactttta ctttagaaaa ttggagaaat tgatcgctta
       61 agataaattc aggacactaa atagtacagg aagtagacta aaacttttaa aagttttaag
      121 tctatctatt tggcaagttt aaaaaataaa aattcggtat caaaaaactg ttaccgataa
      181 catttttgaa agcgttgtct tttgcttctc tatcacttat agtgatgacg tcatattaaa
      241 acacaaggag gaaatcaatg gatacagctg caatttatca tcgaccggaa agtgaatatg
      301 catttctata tacgaataca agatttcgta ttcggcttcg tactaggaaa aatgatatca
      361 aaaaagtata tgtgctatgt ggagatccat ataccataac aactgaaaaa tggtatcaaa
      421 aacaacgacc aatgaaaaaa tgtctaagta ccaatgttca tgattattgg gaaattgaag
      481 tgacgagcga aacaagtcgc ttacagtatg cgttccatgt tgttggggaa gataacacgg
      541 actgttttta cggtgatcaa ggaatttttc cttatcgaga aaatgtgatt actgaaccaa
      601 atttttattt tcgaatacct tattttcatc aaatcgatcg attcactatt ccagaatggg
      661 taaaagaaac agtatggtat caaatttttc ccgaaagatt tgcaaacggg gacaaagcaa
      721 atgaccccga acacacttta ccttggggaa gtaaagatcc agaccgagaa gactttttcg
      781 ggggagattt gcaaggtgtg atggatcatt tggattactt gacggacctt ggcatcaatg
      841 gcatctattt ttgtcccata tttaaagcca catccaatca taaatacgat acgattgatt
      901 attatgaggt ggatcccgct tttggcgata aaaaactttt aaaaaacttg attgatgaag
      961 cacataaaag agggatacgt atcatgttcg atgctgtatt caatcatatg ggtgtccatt
     1021 caccacagtg gcaagacgtt ctcaaaaacg gagaaaaatc gatctataaa gactggttcc
     1081 acatccgttt tttcccagta gattcttatc agatgactga cttaccagaa actgctgaaa
     1141 atatcccata tgatactttt gcttttactc cattcatgcc gaaactaaat acagcaaatc
     1201 cagaagtgca aaaatatttg ctggatatcg caacttattg gataaaagaa ttcgatattg
     1261 atggatggcg tcttgatgtt gctaatgaag tcgatcatca tttttggaaa aaattcagag
     1321 aagcagttac agaaatcaaa ccagacatct atatccttgg agagatttgg cattcctctc
     1381 aagcttggct gcaaggggat gaattccatg cagtgatgaa ttatgcattt acagattcga
     1441 ttaaagatta ttttgcaaaa aagaaaatca cagcttcaca aatggtcagt ggaatgaatc
     1501 atcagcaaat gctttacaga gatcaggtga acgaaggaac attcaactta ttagattctc
     1561 atgatactgc caggatcttg accctttgcc aaggtaacaa agaattgatg aagtctgtga
     1621 tggcctttat gtttcttcaa aaagggtctc cttgtatcta ttacgggaca gaaataggga
     1681 tgactggtga agatgacccg gattgccgca aatgtatgat atgggagaag gaggaacaag
     1741 atctagagct attcggtttt ataaaagaac ttgtttctct aaggaaacag ttgtctaaga
     1801 ttatttctga aggttctact cagtggctaa tagtaaatga tcgtgaagat aagctctatt
     1861 tcacacggaa actagaggga cagatcatct atgtttactt taatcagtca aaagaacctt
     1921 gggtagtaga acaggaaaat gaggtgattc ttagtcaaaa ttgtcagttg ttagaagacg
     1981 ggaaagcaga gatacgacaa tacggatttt tgatcatgtg ttgaaaactg ttaccggtaa
     2041 caacaagaaa gcgtttgcaa atgaagattt gtgcccatta taataagggt gtaacagcac
     2101 gttgaataca aaggaggaaa tgaagatgaa aaaaagaagt gtgaaacagt tgggtttagg
     2161 gttaatctct ttaggaatct taggcggatt agcggcttgt ggcaatggta actcttcagc
     2221 agaaggggaa gccgatgaag gaaaaacgtt aacagtttcc gttgattctg gatataaaga
     2281 ttatataaac gaaataaaaa acactttcga aaaagaaaac gatgtgaaaa tcaagctagt
     2341 agaaaaggac atgttcgatc agttggaatc attagcgtta gatgggcctg ctggaaaggg
     2401 acctgacgtt atgatggcag catatgaccg gatcggtgcg ttaggacagc aaggacatct
     2461 ggcggaagta aaactgggaa atgaagcagc ttacgatgaa acagacaaag cccaagtgac
     2521 ttatgatgga aaaatctacg gagaacctgc agtaatcgag acccttgttt tgtactataa
     2581 caaagattta gtcgatacag caccatcaac gtttaaagat ttggagaatc tatccaaaga
     2641 cagtcgtttt gcttttgagt cagaagcagg aaaaaataca ggatttttag ctaaatggac
     2701 tgacttttat tattcctatg gtttaattgc aggttacggt ggctatgtat ttggtgatga
     2761 tggcacaaat ccttcagata tcggtttgaa taatgcaggt gctgtagaag gaatctctta
     2821 tgcaacagat tggttcaaaa atgtttggcc aaagggaatg caagacataa aaagtgcagg
     2881 agacttcgcc agccagcaat tcatgtccaa taaaactgca gcaatcattg acggtccatg
     2941 gcaagcgcaa acatacaaag aaaacaatat caattatggt gtagccaaaa ttcctactct
     3001 aaataatgga cagccttatc aaccgtttgg cggaggaaaa ggatgggtag tcagcaatta
     3061 tgctaaaaac aaagacctct ctcaaaaatg gcttgattat gtaacgaatc aggaaaatca
     3121 agaaaaattc tttgaaatgg taaatgaaat ccctgcaaac caacaagcta gggaaacagc
     3181 aaaggctaaa aacgatgaat taacaacagc tgtgattgaa caatatgaaa cagctcaagc
     3241 gatgccaaac atcccagaaa tgggcgaagt ttggacaggt gcagaaaact tgatgttcga
     3301 tgcagcatca ggttcaaaaa caccaaaaga atccgcagat gaagcagtaa aaacaatcag
     3361 tgaagccata gaacaaaaat actgattaaa atgataaccg tccattttta taaaaaatgg
     3421 aacaggtgct tcagataaaa gtggcaagag cgagatggaa aattatcttg ctcttgttgc
     3481 cattatctta aaagcagttt gcggtttagc aagtttagag ggggcattca gatgcagcaa
     3541 gataaaacaa gcaatatcaa cgaaaaaaaa atcacgcttt gttcacttat tccagggatg
     3601 gggcagtttg taaatggcca agcttttaaa ggtatcgttt ttttcgccat attcgtccta
     3661 ttcattggtc agatgcttct gggcggattt catgcattgg taggattggt tactttggga
     3721 agtataccaa tggaagatca ttcattattt attttgattg aaggcacttt gcaattcatc
     3781 attacaatca ttttcttgat tttttacggt ttgaatcttt tagacgctta tcgagtagcg
     3841 aagatgcgtc aagagggcaa aaaaatcaat cggacgatga aggaagtcat acataatata
     3901 ggtgatgaag gttttcctta tctattgaca cttcccgctt atcttctcat gatttttacg
     3961 atcattttcc cagtcctagt tacattgttc actgcattta ctaattatga cttcaatcat
     4021 attccaccag ccagtttaat tgattgggta ggggctaaaa acttttttag tatcttcttt
     4081 ttaagctcat atcgtaatac tttttttgca gtcttttcgt ggaccgtgat ttggacttta
     4141 tgtgcaacaa ctttgcaaat cacccttggt attctcacag cggttgttgc tcaccagaaa
     4201 tttattaaat tcaaacgtat tttcggtatc attttcttat taccatgggc ggtcccagct
     4261 tttattacta tcatgagttt ttcgaatata ttcaacgata gcgtaggtgc catcaatact
     4321 caagtgattc ctttcataaa ccagcttcct tttgtagata tcagtgcaat ctcttggaaa
     4381 acggacccga actggacgaa agtagcaatt atcttagtgc agggctggtt aggatttccc
     4441 tatatctatg tgatggttac aggaatctta caatcgattt cagaagactt atatgaagca
     4501 gcaaaaatcg acggagcaaa tgcatggcag cgcttttcaa acatcacgct tcctgctgtc
     4561 ttcctagtgg cagcgccaac atttattaca cagtatacgg ggaatttcaa taatttctcc
     4621 atgatctatc tgtttaacaa tggcggtccc ggaagcgtag gcggaggagc tggttctact
     4681 gatattctta tttcctggat ctataaactt acgactggaa cttctccgca gtattctatg
     4741 gcttcagcaa tcacgttgat tatctccgtc atcgttatct gtgtgtcatt gctggtattt
     4801 aaaaagacaa atgcgttcaa tatgggggat taacaatgaa aaaatttcat tcgagtgctc
     4861 gattccaaaa acgactttct cactttttta cttacttttt tcttttaata ctgtcagtca
     4921 ttatcatcta tccgcttttg atcacagcaa gtactgcctt caagtcagga aatattgcag
     4981 cttttagttt agatctttca agtgactgga ctttaggaaa ctttactcgg ctattcagtg
     5041 aaacacttta cgggacttgg tataaaaata cattgatcat ttctgtccta acaatgatcg
     5101 tacaagtgac tgtagtaact ttggcgggct atacttatag tcgctatcgt tttatcggcc
     5161 gcaaatcaag tttgatgttc tttttagtga ttcagatggt accgactatg gcagcattga
     5221 ctgcctttta tgtcatggct ttcttattag aagcgctcga taagtactgg ttcttgacaa
     5281 tgatctatat aggcggtgga attccaatga atacttggct gatgaaagga tactttgaca
     5341 ctgtaccaat agatttagat gaatctgcta aacttgatgg ggcagggcat ttcaggattt
     5401 ttttccagat tgtattaccg cttgtcaaac cgatgctcgc cgttcaagca ttatgggctt
     5461 ttatgggacc ttttggggat ttcatgttag ctaagttcct gcttcgaaca cctgaaaatc
     5521 agactgttgc tgtcggctta caaacattta tttctgatgt gacaaaccaa agagtctctc
     5581 tttttgctgc tggggcaatt ttgattgcac taccgatttg tctattattc ttctttttac
     5641 aaaagaactt tgttaccggc ctgacatctg gcggaacaaa aggataggag cagagtatca
     5701 tgcaaacatt tccgttaaac tatttttccg cattacgtac gccaactcaa gtgtttgcgg
     5761 gacgcaaact attatcatgg cctaaatttt tcttgatatt tgtattttta gtttccttga
     5821 tggtcatgcc tgtgactctt ttttatgcca atcagatcca agcgattcca ttagaacaat
     5881 ttctatccgt tcattcgctg atcgatgaac aaggaaccca aaaattttct gaacttgaat
     5941 tatctgaaac tgggttacaa gcatcgcaac aaacgatagc tgtgacccct gaaattttgg
     6001 taggtgtgtc tttatctgag aaacaacaat ccgatcatgg aacttttatt gattttgaga
     6061 aggaacaatg ggtgatccaa caaaaggata aatctggtat acgccgctat acgatgaatt
     6121 atagcccatc tttccaacca gattctgtac gtacgcccga agatttccaa cgatttttag
     6181 aaagagaatt ttatgccagt aatcgtccaa caatcatttt atcttatagt ttaagtcttg
     6241 gtcttgtcct atttgtcatg acgagtctca ttttattcgg ggcttccttt ttcttgtgga
     6301 tgacaagaaa aagccaactt tcatccattc atacattcaa agagagtgca aatttgatgc
     6361 tgaatgttat gggaataggt tccatggcag cagcagttgt agggtttatc cactttgact
     6421 ttatcttgat gctaagtgtc caaacaacga taactgtctt gctgctttta tgggtttttg
     6481 tcaaaacaag attcaaagat aagcgtgtcg aatgacaagt ttaagcgatt tatgcgacaa
     6541 tgaatgtgaa aaaaatgaaa aataaagtag tttttgaaaa atttctgaaa aaattaaact
     6601 tcttagttga ttcttttctt ttttagtatt aacgtaatag ggtagttttt ttaaggagga
     6661 aaatcatgtt agagaaaact ttatacaatc aattattttc acgttctttc tcgctccctg
     6721 tcgaagtaaa ttattgggac ggtacaacga aacaatacgg agatacggac actcctcccc
     6781 aaatcaaaat caatatccat gaagaaatcc caatgaaaga gattacaagt aacgcatctt
     6841 tggcactagg agaagcctat atggatcatc gaatcgagat cgagggaagt atccaagcat
     6901 tgatcaacga tgcttatcag caagcaaaca gctttttacg aggaaaagat tatctaaaat
     6961 ggctgccaaa gaaagaaaaa catacgaaac aaaaaaacaa agaagatatc catgcgcatt
     7021 atgacttagg taacgacttt tataaacttt ggctagatcc aactttgact tattcttgtg
     7081 cttatttcaa tacacctgaa gatacgttgg aacaagcaca aatcaataaa gtccatcata
     7141 tcttagacaa actctttatc aaagaaggcg aaacgttgct tgatatcggt tgtggctggg
     7201 gaacattgat gttcactgct accaaagagt ataatgtcaa ggcaactggg atcacactaa
     7261 gtgaagaaca atacgatttg attaccaaaa gaataaaaga agaacattta gaagataaat
     7321 gccgtgtatt attaatggat taccgcgaac taaaaggcga aacgtttgat catatcacaa
     7381 gtgtaggtat gttcgaacat gtaggttctg aaaatcttga aggttatttc aaagtggtaa
     7441 aagatttatt gaaaccaaaa ggaacagctt tgatccatgg aatcagccga caacaaggcg
     7501 gtgcgacaaa tgcttggatc aataaatata ttttccctgg cggttatgtc cctggtgtta
     7561 ctgaattagt tgataatatt acaaaaaacg atttgcaact gattgatttg gaaagcttac
     7621 gtcgtgacta ccaattgaca ttagaacatt ggacaaagaa ttttcatcaa gtccaagatc
     7681 aagtcacgaa agaaatgggt gattccttct atcgaatgtg ggatctatat ttacaagctt
     7741 gtgcagcttc atttgaatca agtaatatcg atgtgattca atatttgctt gttcaacctg
     7801 gaaacaatga catcccaatg catcgtgcat aattgaggca aaagtactgt ttaaagacta
     7861 tttaaaaaga agctaagttt tatgatgctc cagaagtgaa cttttttgtc taacttctag
     7921 gattgtctct aaagcttagt ttctttttct ttatattttc ataataaaag tttggttctt
     7981 taatttttaa acatgacaga aaattttttc tt
//
LOCUS       DAJSKS010000123         7887 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJSKS010000123 DAJSKS010000000
VERSION     DAJSKS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.127"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..510)
                     /locus_tag="ON396_002290"
     CDS             complement(<1..510)
                     /locus_tag="ON396_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCR3114350.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRI"
     gene            complement(609..959)
                     /locus_tag="ON396_002291"
     CDS             complement(609..959)
                     /locus_tag="ON396_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HCR3114351.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1116..2645)
                     /gene="ahpF"
                     /locus_tag="ON396_002292"
     CDS             complement(1116..2645)
                     /gene="ahpF"
                     /locus_tag="ON396_002292"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR3114352.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(2655..3218)
                     /gene="ahpC"
                     /locus_tag="ON396_002293"
     CDS             complement(2655..3218)
                     /gene="ahpC"
                     /locus_tag="ON396_002293"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCR3114353.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(3362..4024)
                     /locus_tag="ON396_002294"
     CDS             complement(3362..4024)
                     /locus_tag="ON396_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3114354.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            4208..5854
                     /locus_tag="ON396_002295"
     CDS             4208..5854
                     /locus_tag="ON396_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR3114355.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            5845..6927
                     /gene="uxuA"
                     /locus_tag="ON396_002296"
     CDS             5845..6927
                     /gene="uxuA"
                     /locus_tag="ON396_002296"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR3114356.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(7035..>7887)
                     /locus_tag="ON396_002297"
     CDS             complement(7035..>7887)
                     /locus_tag="ON396_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HCR3114357.1"
                     /translation="KDVQKEFTEYEYIDFFIDLSVDILRPNLPICREQRILVNILARL
                     SITKIESEETSLEQYKLLKKANVPHVLYSREMVESLCTVFDLFIPEKEKILYSLAFAR
                     NKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILEKE
                     NVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQNDFM
                     ENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQSK
                     KENQVTS"
BASE COUNT         2504 a         1468 c         1453 g         2462 t
ORIGIN      
        1 tattctgatg aaattgataa tctcctggat gcccatgaaa atcaaaataa agccgaatat
       61 ttggaataga agcatcaaac ttttgaatgg attgaacaat aagacgaatc cgccaatcaa
      121 caacagaata ctactataaa cactccaact tttagctttt gtattcatgc tgaagaacaa
      181 ctggaacaag ctgttgatga taacagctaa tccaagaaga atcggcagga tagaaacgat
      241 tgcaggtgca aaagcataga cgatcagagc taagatcagg aaaaaaagac cactgaacag
      301 accgatactc caagaacctg ttttcctttt aaaacggtaa gcctcaaaaa gattcaacaa
      361 cccaagcaaa acaagatagg cggtgatcag ataaccgata aaatgaaaaa cagcagaagg
      421 gtttagcaca atcgcaatcc cagaaataat ataaacagca gctcttaata aggcgtattt
      481 ttgaatattt tgtttaatcg tctcaaacat ataagaaaac ctcctttgac ttattttaac
      541 gtgaactagt cattttacca aattaaatgt tacaaaatga gcgaaggaga agaatatctc
      601 tggaaaaatc aacttctgag gatcttttcc atttttttgc cttttgctag ctcatcgatc
      661 attttatcta aataacgaat ttctttcatc aagttatcct cgatgtcttc tactcgtaca
      721 ccacaaacaa cacctgtaat caaggaacgt gaaggattgg ggacaggggc ttccgcaaaa
      781 aattgttcga aagtcacttg tttttgtagt tgatattcta gttctgtttg ggaataaccc
      841 gtcagccaac aaatgatttc atctacttcc tcttttatac gatcttttct ctccgctttt
      901 gctacataca taggataaac gcttgcaaaa cttgtcgtaa aaatacgatt ttgcgccata
      961 atcagtcacc tttctttttt tcaaattatt tactacttat agtatataag aaaaagagaa
     1021 gaaaaagcga ttattcaaaa aaacaaaaag gattgtgaca aaattcttgt caccatcctt
     1081 ttttccgctc tctgactttt tacaatcttc atagctcaag tgcgcatcag ataatcgaat
     1141 gctcctaaag ctgcagtagc tcctgaaccc attgaaacaa tgatttgttt gtatggacta
     1201 tctgtacaat caccagctgc gaaaacgccg gggatattcg ttgcgccatt tttctcggtc
     1261 actatttcat ttcttggatt catttcgatc gttccatcta gccaatccgt attagggagt
     1321 aagccgacta gaatgaatac tcctgaaaca gctagtgtat gcatctccat cgtacggcga
     1381 tcgatatagg agagagattc tacctgattc gtaccgctga tttctttcgt cgctgcatgt
     1441 ttgataacag tgacattcgc aagagagact agtttatcct gtaagatttg atctgctttt
     1501 aattctggta agaattctag aacatataca tgttttgcta acccagccaa atcgattgct
     1561 gcttcaacac cagaattacc accaccgata acagcaatct cttttccttt aaataaaggg
     1621 ccgtcacagt gtgggcaata agcaattcct ttatttttaa actctttttc gcctggtaca
     1681 ttgatagaac gccagtgcgc acctgtcgcg atgatggcgg tttttgcttt aagtgatata
     1741 ccgttagcta agccaatatt aataaaatgt tgctctttat ttaaggaaac ggcctgttga
     1801 ttcatcataa tgtcgatagg gtaactacgc acatgacttt ctacttgctg catcaatttt
     1861 ggaccttctg tgtaagaggt accaatcata ttttcaatcc ctagtgtttc aacgacttgt
     1921 ccgccgaaag aatcagcgac aagtcctgta cggattcctt tacgtgctgc atagatagct
     1981 gcactactag ctgctggtcc accgccaatc actaatacat cataaggttc tttttcaaac
     2041 aactcttctt caatcaaagg gccagtgatt ttttcaacaa tttgttcgat cgtcatgcgt
     2101 ccgctactaa attcttctcc atttaaaaac acagttggca ctgccatgat ttttttatca
     2161 tcgatttctt tttggaacat cccaccttca atcatcgtat gagtaatatt tggatttaat
     2221 acagctaata tattcaatgc ctgtacaacg tcggggcaat tatgacaagt caagctaaca
     2281 taactgtcaa aatgcaatgt ttgtgttatt tttttgattt taagactcaa agattcactg
     2341 attttcggtg cccgtccact aatttgtaac atagccagta aaaaagagga aaattcatga
     2401 ccgaggggga taccagcaaa aacgattcca ctttctttgc caactggatt gatagaaaaa
     2461 ctaggtgtgc gcatcgaatc tcttttttcc caagagattt taggagacat ttctgctatt
     2521 tccttcgtaa attcagctag tttttgacta ttttcactat tatccaaact aacagaaaaa
     2581 acaataggag attcaatcag tgctaaatat tgttcgagat tttgtttagt ttctttgtct
     2641 aacatatcca ccacctaaat ctttccaaca agatcaaaac ttggtttcaa tgtttcgcca
     2701 ctttctttcc attttgccgg acaaacttca cctgggtggg tacgtacata ttgagcagcg
     2761 cgaatcttat caatcagtgc actagcgtca cgaccgattc catctgcatt gatttccata
     2821 gcttgaacga ttccgtctgg atcaataata aatgttcctc gctgagctaa tccttgctct
     2881 tcatccaaaa catcaaaaat acgagaaatc tgatgagacg gatcaccgat catcacgtat
     2941 tcgattttac cgatagcatc tgatgtatca tgccatgctt tgtgagtaaa gtgggtatcg
     3001 gtagagcaag aaaagacttc aacacctaaa gatttcaatg tactgtattg ttcttgtaag
     3061 tcttctaatt cagtaggaca aacaaaagta aaatcagctg gatagaagca aagaatactc
     3121 cagttcccca taagatcttt ttcactgact tctaaaaatt ctccttgatg ataagcatgt
     3181 gcattaaatt cttcgatttt tttcccaatc aatgacatag taaaacctcc aataataatt
     3241 atttatttat aattattgta atttagttgg agttgcaaag tcaatggata ttttgttatt
     3301 taataaagat tactcaaaaa cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat
     3361 gctactgctg gaaatactcg ggataacgtt gagtaacagt ttcctgttct ttcagcatca
     3421 tgtgtaagtg caatcccgtt tgaaatcgag cttcttctgg ttttttatca actaaagcct
     3481 tgagtaaatc ataatgttgt tccatgatcg tttcccatgg aagcccctga atggtcactc
     3541 gtaaccagcg gaaacgttct aaatgggtat tatggtcatc taaccagttc caaacttctt
     3601 ttcttcctgc aatttcgaaa caagcttcat gaaataaatt atctgcacgg aaaaaagcgc
     3661 gttcgtcatt tattttgact gctttttctt gttcttctaa aatcgtttct agcactattc
     3721 gtgtttgtgt gtccaatttg gaacaacatt caatcatgat ttgctcttcc aactgttctc
     3781 ggacaaagcg agcattttct gcagaaacaa gattaatttt ggaaacatac gtaccacttt
     3841 gaggtatcgt atacacgagt tcttgctgac gtagttgaat caatgcttcg cgaatgggag
     3901 tccggccgat agcaagatgt tcctccagtg ccttttcaga tattttcctt ccaggttcta
     3961 agtcagcata gataatttgt ttacgaatcg cttgataagc ttgtgattgc aagctgcttt
     4021 tcatctttat tcctccaaaa tcatttctat gaacgtagta taacagaaga ggtctgaaaa
     4081 acaatctggt atgtcacatt aactagtaaa tttgtataaa tagtaaaatt ttggttgaca
     4141 tagcgatttt tattgcataa aattcacttg aatacaaaaa ctaatatgtt agtttaggag
     4201 gggaaaaatg attgatttaa acaactggag gaactttatc cctgaattat ctcaaaaaga
     4261 agtagaaata cctcattttg accgtgcatc attaagagaa aatggacaaa aaaatcctgt
     4321 ctggatccat ttcggtggcg gaaatcttta tcgaggattt catgcagaaa tcgctcaacg
     4381 tttagcggat ctaggcgaat tgtccactgg tgtcattgtt tgtgaaacat tcgatgaaga
     4441 aataattgaa agggcttacc aagattttga tgatttgatt cttgaagtgg tcatgccaag
     4501 cagtggagag ttcgtcaaac gtatattagc tgcgacagct gacagtctgt attgtcatcc
     4561 agattgttct gaacactttg ataagatgat tcgacacttt gaaaatccta gtttacaatt
     4621 agttactttt gctattactg aaaaaggtta tcgattgaaa gatacaaata ataaatatat
     4681 aggaccgatt gcagtcgata taaaaaatgg tccaaagaag ccatcacata caatgagtat
     4741 tcttgctgtg ctactttata aacgtttttt atctggagct tggccgattg cacttgtctc
     4801 tacagataat ttttcacaaa acggtagtca attacgtact tctttgctaa cgatcgcaaa
     4861 agaatggcaa aaggaaggtt atgtacaaaa tacatttatc caatacttat cgaatgaaga
     4921 aattgttgcg tttccatgga cgatgattga tcgtatcacc ccgaatcctt ccttgtcagt
     4981 tagcaaaatg ttggcaaaag agggcttttc aaatatgtcg attttccaaa ccgcaaaagg
     5041 aaataatatt gctccatttg tcaatacaga ggacatccat tatttaatca tggaagatca
     5101 ttttcccaat ggacgtccga aattcgaaaa agctggggta ttcttgactg atcgtgcaac
     5161 ggtagataaa gcagatacga tgaaggtgac gacttgcctt aatcctttgc atacgtcttt
     5221 agctattatg ggttgtttac tggggtatag gagcatagct gaggagatga aagatccaga
     5281 tttatcgctg ctagtttctg aaatcgggta caatgaaggc ctcccagttg tggagtctcc
     5341 aggtatcatt gatccaaaga tctttctgga agaagtaatt actgtgcgct tttctaatcc
     5401 aaatattcct aatatgcctc agcgaatcgc cacggatacc tctcaaaagg tggcgattcg
     5461 ttatggtgag acgattaaaa aatatatgga aatagaagtt aaagacccgc agacacttgt
     5521 attcatacca ttgactctcg ctgcttggct tcgatacctt attggcattg atgatcaagg
     5581 aaaaccattc atcccgagtc cagacccact attgacagac cttcagcata agctttccga
     5641 tattcaactg ggtacgcaag atacatcaca tattcatgag aaaatggctc ctattttgtc
     5701 aaataaagaa atttttggtg tagatcttta ccaagcagag cttggcagaa aaatagaaga
     5761 aatgttcatc aagatggtag caggaccaaa tgccgtacga aaaacattac aggattactt
     5821 acaagaacat ggaggaaatt tatcatggaa atgactttta gatggtacgg atcacaggaa
     5881 gaaaagatta ccttagatga aatcagacag attcctgcgg tcaaagggat tgtaggcaca
     5941 ttgatggata taccagtagg agaggtatgg cctagggaga ggatcaaaga actaaaagaa
     6001 gaaattgagt ctcgtgggtt aacactaaaa gtaattgaaa gcgtcaatgt gcatgatgcc
     6061 attaaaattg gattaccttc ccgtgagcag tatatcgaaa actataagca gacaatccga
     6121 aacttggcag aatatggaat agaagtcatc tgctataatt ttatgcctgt atttgactgg
     6181 gtgaaatctg accttgacta ccgcttagaa gacggctcat ccacgttagc ttttatatct
     6241 gatgatattc cagatgatcc aagagaaatc gtcgcgcgca tcgaacattc cagcaatgaa
     6301 tttggattgc cgggatggga acctgagcgc ttagctccta tcaaatcctt gcttgaagcc
     6361 tatgcatcag tagacgaaga aaaacttcga gaaaattttg cctattttct ccaatcaatc
     6421 atcccaacat gtgaagaggt aggggtcaaa atggctgtac atccagatga tccgccatac
     6481 tcgatttttg gattagctcg aatcgtaaag aatcgagaag atttagattg gttgtgtcgc
     6541 atagtagaca gcccttataa cggaatcact ctttgtacag ggagtattgc agaagatcca
     6601 gaaaatgatg tatatgggat tttagcagaa ttttgccaaa gagatcgaat cccatttgca
     6661 catgttcgaa atattaaatt tttagctaat cgagatttct atgaggcacc acataaatcc
     6721 gaatacggtt cattagacat gtaccgcata ctaaaaacaa tgtatgacaa cggatttaac
     6781 ggatacatcc gtcctgatca tggaagaatg atctggaatg agacaggaag accaggatac
     6841 ggcttatatg atcgagcatt aggtgctgtt tatctaaatg gattatggga agcaatcgaa
     6901 aaaggaaaca aaagtaaaga taattagtga aaaaaactgt aattttatta tgtaaataag
     6961 aaggatgttc aaattcttga tacataaatc cggaggttgt taataagcat tcggatttat
     7021 gtatcttatt tggtctagga agtaacttga ttttcttttt tcgattgatc catgagtgct
     7081 tcttctttta attcaaagat atattctgag atcctcataa tcactttttt ccagtcttct
     7141 ggtgtgatga ttccttttaa aatatagatt tttttgatat gagaaggcaa ttgtttcaaa
     7201 gggcagtcgc tgataacgat gttgcagttc tccataaaat cattttgtat tttgatcgat
     7261 tcatttgaaa aaatttgttc tatcttaacg atcagatctt tactgatata aaaatcacgc
     7321 gtatagttca caccaatcac aattcgcggc tgttttttgt agcgatcata caaatgtact
     7381 aggtcttcaa ccatccattg cacattttcc ttttctaaaa taactggtaa caagtgttga
     7441 ttttgttctc tgaaatccat ataaaattgt tcaatggcgg tttggacttc tttcaatacg
     7501 ctatgttctt gataaagcat gaaagtagga aggggcgtat ttggttgatt gaagttaaga
     7561 aatccattat aaagtttatt tcgtgcgaat gccaatgagt aaagaatctt ttctttttca
     7621 ggtataaaca aatcaaatac agtacaaagg gactccacca tttctcttga ataaagaaca
     7681 tgaggaacgt ttgctttttt taaaagtttg tattgctcca agcttgtttc ttccgattcg
     7741 attttcgtga tagaaagtcg agctaaaata ttcactaaga tgcgttgttc gcgacaaatt
     7801 ggcagattag gtcgcaaaat atctacagag agatcaataa aaaagtcaat atattcatac
     7861 tctgtgaatt ctttttgtac gtctttc
//
LOCUS       DAJSKS010000124         7726 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJSKS010000124 DAJSKS010000000
VERSION     DAJSKS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.128"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(67..1488)
                     /locus_tag="ON396_002298"
     CDS             complement(67..1488)
                     /locus_tag="ON396_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3114358.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1731..3608)
                     /gene="tdc"
                     /locus_tag="ON396_002299"
     CDS             complement(1731..3608)
                     /gene="tdc"
                     /locus_tag="ON396_002299"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCR3114359.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3899..5155)
                     /locus_tag="ON396_002300"
     CDS             complement(3899..5155)
                     /locus_tag="ON396_002300"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR3114360.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(5267..5472)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5735..7036
                     /locus_tag="ON396_002301"
     CDS             5735..7036
                     /locus_tag="ON396_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR3114361.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(7105..7704)
                     /locus_tag="ON396_002302"
     CDS             complement(7105..7704)
                     /locus_tag="ON396_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3114362.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         2285 a         1587 c         1359 g         2495 t
ORIGIN      
        1 aactattcac ttggcaggac ctcctactaa atcagcgaca aaacgtcggc tgaaagactt
       61 atttttttat ttttcttcta aacttttttc tacctcaatc ccccaagaat ctttcttgaa
      121 gtgattgatg atcaatggaa taacgaacat gaccactgtg atagctgcta ctaataatgc
      181 atcactcaat gtcgctgtac ctaatgtagc aaatacgaca acgatgatag agaagatcag
      241 catgcagctg actacccaag ctaatccgtt tccgttttta ccaattcggt aaggacgttc
      301 gaattctgga tgtttcttac gcattttaag caatgaaaca gcaatcaaaa tgtacacgat
      361 acaataaata acagttgtag catttgtaag tgtcaagaat acagcactta cgtcatccat
      421 tacaccataa agcaaagcaa ataatgaaat aacgattgat tgtgttagaa cgacatttct
      481 agatacgccg tatttgtttt ctttatggaa tccgaatttt ggtggaagga atccttcacg
      541 agctacttgg atgattgttt ttgatggtcc agtaacccaa gctgataatt gtaaaagcac
      601 accaataaat accatgaagc tgaagatatt tccgatcact tctggtaagc ctaggatttg
      661 acagtcaatc aagataggct gcgtgatatt tgctaattcc atttttccat ctggtacgac
      721 atttgctaca agcatcgcat tgatgacatt caataaaact aatccgatca aagcgataaa
      781 tacgcctttt gtataatttt ttgttgcgtc tttcaaacga ggcatgtaaa cagaactcat
      841 ttcgatccca acaaaaataa atgtgatacc agctaagtat ttcaatgaat caatatgttc
      901 caagtcaggg aaagcttttg accatgaaaa gtcacctaga tagccgtttg agactagtcc
      961 gactttgaag gctgccaaaa cacctaaaac gaacatgatg actactggga tatatacccc
     1021 tagccataca ccgatgttcc cgccgacttt tgccatatcg aatttcaagt tcaagatcgt
     1081 gatgatccag tagataacta aaatacaacc taaaacgaac cagtgattat tgccaagttc
     1141 tacattacca aatgtgtttc ctaacaatgg tcctaaagta gaagcgacca ttaccatacc
     1201 tgggaacatt tgcacccata atagccatgc aacgacaaat ccccatttgc tgcccagtgc
     1261 tgtttttacc cataactgag gaccgccttc ttgaggaagc atcgtggaaa gctcaccgga
     1321 aaccattgat atcggaccgg caaaaaacaa aactgcaaaa attgaataaa aaatcaatgt
     1381 ccaaccgact gcggctaacg taggaatact ccgaaccgtt gcacacagtg ccattgtcag
     1441 accgataaag gtcccaagac ccattttact tacttcagat ttactcaccg ttgacactct
     1501 ccttgatttt ttggataaaa taacggtgtc tttgaagtag tttcagcaat aaacgaaaca
     1561 attcagcgaa tatttgaggg gattctttga gtttattgct tgaagggttt cttcaaagac
     1621 aacctcaaaa caaagggatc gaagagctta ggtctcccct tgctcttcag atccaaacat
     1681 gcgtcagaaa cagttagtat tcaagctgtt ttctaacatg attaccttgc ttattttgct
     1741 tcgcttgcca ataattggtc tgcgtagatt ttttccaatt tttcttgaag agctgctttg
     1801 attttttcag catattcttc aaagtgttct tctttgttca tgtatggtgt cattacgctg
     1861 gcacgcaata cagttacttt tccggcacgg ttccattctt catctgagaa tcctagttgg
     1921 ttcacaaatt gtaatgggct gttgccatag tctgggatag caaagtcagt atgtgaagtt
     1981 aagaactcat taccgtaaat gcttcctttc acgtatgaag aataatcata tacgtcatgg
     2041 ttcaatttat tcattgcaac taagtcatcg ttgccttttt ctttgaatac atagtctacc
     2101 atattgaaat ctggataagt taatggatga acttcgattt ctttgtctcc tactttaaat
     2161 gacaagtcat tcaagaagtt gtagaaacga tgtgcacctt cgattgatgc acccattaat
     2221 ttaccatatc ctgtgacatt caaaggtaag acatggtgtg ctgcccaaac gctggctgct
     2281 gttgcccctg cttttgaacc ttccaagata taagccccaa gtaatgctgg gatatctgcg
     2341 cctttttcaa atacatatgt cgcaaagtaa gagataacgt ctctcatgcg gatatctttg
     2401 atgacgatac caccggcaga atatggaaca tagcccattt tatgtgggtc aatcgttaca
     2461 gattctgctt cttcgattgc tttatatgca ctgtgtactt cttctaagat ataatctttg
     2521 ttttctgtaa agacattgta tttgtaatga acatctttta aatcttcaaa tggaatgaag
     2581 ttgttgtctt cgtctaagaa aatcgcacgg ccgtatccgc cataagcagc atctacgtgt
     2641 aagtagaagt aaataccgtc tttttctaat acgcgacgca atgcaacgat tttatcgata
     2701 ccgtcgattg ctccttcttc tgttgatcct acaacaccga ctacacctag gattggtgtt
     2761 ttttcagctg ctagaccacg aacgattttt tcaagttcgt tgatgtccat gcgatagttg
     2821 tggtcaactg gcactggaat cacttggtct aaaccgatac cgatgatgtc ggcagctttt
     2881 agccatgaat agtgtttagt ttgtggaacc aaccattttc ctaatttttc taaatgtttt
     2941 ccgctacgtg ctgagtgagc tttgatttcg tcgatttttt ctgggacgct gtctaacaga
     3001 ttcatgattt cttctgttga caagttcatt aattcccaat cgctttttcc agcaactagt
     3061 tcaggtgtca cttctttcat tgcaagcggc aatgatttga tgttgcgggc ataccaaagt
     3121 ccttctaagt ttgctaaaga accatcagca acgatgtggc cccagccatc tttatagctc
     3181 attaattttg caaattccat tcctacttct tcttccattt ggcttgttgc aggagaagat
     3241 tcataggcaa cgttattacc attccatagc atagcaaaat tgtacgctag taaagatggc
     3301 atcaatgttt cagagttcat atgaccccag taacgtccgg cattatgcca aggaacggaa
     3361 tgggtacgca tccgcgctga aatttctgat agaacatcct ttgttttatt cacagtgtgt
     3421 tcaaaactag cactgctttt ttcttctggc gtaataatag gcatgtcttg aggcatgtag
     3481 ttttgacgcc agcctaaatg ttcatccacc aattcattca acaaagcttt atagatttgg
     3541 ccgttctcag ctttgtcccc aatgaatagg gcatttaggt ttagatcttt cgacaatgat
     3601 tcactcatag tgaaaccctc ctaaaaaaat atttaaattt tgtcgagccc tacattacac
     3661 ctgttcttct attctgtaaa ctaagttttg gcctgatttt taatcatatt ttaatatgta
     3721 tcaattatcc gaattgttca cacttttgaa ataattaaaa aaaacgaggg aatttacaaa
     3781 tgtaaactcc ctcgcaaaga ttgtattaac gacaaccagc gttctatttt aggatgggtt
     3841 tcaaaattgg aatgttgtaa tagtatttcc ctttttcaag gtaaaaactt actaaattct
     3901 aatcaattac tttggccaaa aagtaatttt tcttaccttt tcgaataacc acaaacttac
     3961 catcgaattc atcggatgga tttatgacaa aatttaaatc ggtaacgcgg tcaccgttaa
     4021 tacttatagc tccgtttgaa acatcttcac gtgcttgacg tttagatggt tcaatttttg
     4081 tggaaacaag cagttccacg atattttcag gtgtgcttga aatttcaacg ttcggcattt
     4141 taccgaatcc ttgcgcgatt tcttccgcat ttagttcttt aatatttcca gagaacaatg
     4201 cttgcgttat tttttgcgct tctttcaagt cttcttcact atgcacaaat cttgtaactt
     4261 cttctgccaa acggcgttgt gcttcacgtt tctcaggttc tgtctctact tttttcgcta
     4321 agtcttcgat ttcttcttga gacaagaaag taaagaattt caagtatttc actacatcgc
     4381 gatcatcttg attcaaccag aattggtaga actcaaatgg tgaagttttc ttcggatcaa
     4441 gccaaacggc accgcctgca gtttttccga atttcgttcc gtcagccttc agcatcaatg
     4501 ggatcgttaa accgaacgct tctgcttctg gtccttctaa ttttcggatt aggtctaatc
     4561 cagctgtgat atttccccat tggtctgctc caccgatttg caattgcaca tcgtatgttt
     4621 tatgcagatg caaaaagtcg atagattgca ggatttggta agtaaactct gtaaatgaga
     4681 ttcctacttc caaacggctg gcaacgatat cttttgctaa catcgtgttg atgttgaagt
     4741 ttttaccgta gtctctcaaa aattcaagta aagagatttt tgacaaccag tcgtagttgt
     4801 tcacaaatgt gatatttgca tctttaccaa acaattttct catttgattg gaaagtgcgt
     4861 ccacgttatg ttgcacttgt tccatcgttt gcaacacacg ttctgttttg cggccgcttg
     4921 ggtcgccgat cgttcctgtt ccgccgccaa tcagaatata tggatgatgg cctgccaatt
     4981 ggaatctttt catcatcata aatggaatca aatgtccgat atgcatcgaa tcaccagttg
     5041 gatcaacgcc gcaataaagt gaaatgctct tttcttcaac gagctcgcga agtctttctt
     5101 cgtttgtttg ctgattaatg gcgtcgcgcc aagttaattc atcaataata ttcattttac
     5161 tgacctccgt gtagtttttt tatgaaagca ggaacctgct agcatgtgta attaattatt
     5221 tcttcaggat aggataggaa ataaaaaaag tcccttggca tataagccaa gggacgaaat
     5281 ctcgcggtac cacccaattt gtatgtctag atatccaaca tacctctcga acatttaacg
     5341 ctgtcgcgtg gcaatcactg ccaaagactc ctgaaatgta attcgcttta aagcatgtac
     5401 tggtttgcag cgaccaccag ctttctgaaa cattggcttt aacaactact tcgtttcgat
     5461 cttcgtcttg ttattattaa tatgtgtcta tcatagagcg caactttttc tttgtcaaca
     5521 gaaaaaggat atttttttta aattcctcat tttttctttt ttgaatccgc tctaattccg
     5581 aacattttat tttcaattat tgttaaaaga aataactttt tctgtatttt ttctgcaatt
     5641 tatcgagaag tcactttact ttgcctctta ttctttcttc gttacaataa ggaatgggac
     5701 aagtaaaaaa atgaaaaagc aagggacgag gtatatggaa aaaaagcaaa aaattatttt
     5761 aggagccact tctgctttaa ctatcgttat tttaattcta agtttgtttt ttgtcatccg
     5821 atcatctaca aaaaaagaac caatcattcc tgaaaaaaaa ataacagaaa aagcaaaaga
     5881 accgacagat gataagcaac aactagttac tcaagcaaaa aatctggctc aaagctacga
     5941 ttatgaccaa gcgattgcgt tattggaaaa agatagtaag aacggatcta ctgaaacaca
     6001 acagcttctt caaacactaa aaaaggagaa agaacaactg atcgactggt ctgatcctac
     6061 tcagatctct catgtctttt tccacagctt gatcgtggac ccgcaaaaag ccttccactc
     6121 tcaacaggca caaggataca aagattatat ggtgactgtt gaagaattca accgttcgat
     6181 cgaacaactt tatcaaaatg attttgtttt agttaatttg aatgatttga tccaaaaaga
     6241 cgaacaagga aatctttcct ttaacgggct tgcgctacct gaaggaaaaa aaccattggt
     6301 tctttcacaa gatgatgtca gctattatga atatatggat aattccggtt ttcctagccg
     6361 attggtcgta tcaaataaca atgaaatcaa aaatgtctat aaagagaaag ataaagaaga
     6421 aatcggtgac tttgacatgg ttcctctgat cgatgccttt gtcaagaaac atcctgattt
     6481 ttcttatcga ggcgcaaaag gtaccttggc cctgacaggt tacaacggag ttttaggtta
     6541 tcgaacatca aaatctcaat atggagacaa tgaaaaaaca aaaaaagaga ttaaagaagc
     6601 taaaaaagtg gcggaacagt tgaagaaaga cggctggagc tttgcttccc atacatgggg
     6661 tcacttgaat atgactcaag cttcactaga agatgtaaaa aaagacaatg aattgtggca
     6721 gcaagaagtt gcacctatcc taggaaaaac aaatattctg atttatccat ttggtgcaga
     6781 catcagcgac tggcagcctt attcagcaga aaatcaaaaa tttgcttact tgaagagtca
     6841 aggtttcgat attttctgta atgtcgatgc ttctacacca gcttggggac aattaggtac
     6901 ggatttctat cgaaatgctc ggatcaatat cgatggcatt cgttttcaat ctgatttaga
     6961 cggaaaaaat cctattcttg atccattcat caatgtccat gatgtctatg acaaagaagc
     7021 acgcgaatct gcttgatttt acgtcattta tgaaccaaaa aagccgaact gacattttat
     7081 cgtcagtttc ggctttttta tgctttattt cttcttcggt gcgattccct tgatcaggac
     7141 ttctgtccat tctgctgtat atggaagcag ttttgcgtat atctccggct tagaaattcc
     7201 ttctaccatt gcttgaaaat agaggatcaa gaactcatcc gaatatttca aatccacttt
     7261 tccttctttc cgccctcgat ggaaaagatc aagcatgata ctgaaacttt cttgcgtata
     7321 cgcatccatc gcgtcagaaa attctttttt ttcttttttt gtatagtatg tcatcaaatc
     7381 gatataaaat tgtttgttca ctcgattgag ataacggatt ttgttttcac tcatggcaat
     7441 cagtgtttct tcaaatggaa gattttccgc aatcaacgct ttcgcttctt gccctaaatc
     7501 attcataaat gcaaaaaata cttcataaat cagcccttcc ttgctgccaa aatatttaaa
     7561 aatcgtcgct ttacttacct gagcgttctc agctacctta tccattgtca ctccagcggt
     7621 tcctatgtct gtgttcatca agtcaaaagc tgcttgtaaa atctctttct ttttctcttg
     7681 tgctctcttt tcaaaaccgt tcatttgtag agcctcccgt aatttt
//
LOCUS       DAJSKS010000125         7648 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJSKS010000125 DAJSKS010000000
VERSION     DAJSKS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.129"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            18..2345
                     /locus_tag="ON396_002303"
     CDS             18..2345
                     /locus_tag="ON396_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3114363.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2471..2800
                     /locus_tag="ON396_002304"
     CDS             2471..2800
                     /locus_tag="ON396_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCR3114364.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2840..3844
                     /locus_tag="ON396_002305"
     CDS             2840..3844
                     /locus_tag="ON396_002305"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3114365.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3841..4287
                     /locus_tag="ON396_002306"
     CDS             3841..4287
                     /locus_tag="ON396_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCR3114366.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4437..5252
                     /locus_tag="ON396_002307"
     CDS             4437..5252
                     /locus_tag="ON396_002307"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR3114367.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5458..6630
                     /locus_tag="ON396_002308"
     CDS             5458..6630
                     /locus_tag="ON396_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR3114368.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6792..7286)
                     /locus_tag="ON396_002309"
     CDS             complement(6792..7286)
                     /locus_tag="ON396_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114369.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(7283..7477)
                     /locus_tag="ON396_002310"
     CDS             complement(7283..7477)
                     /locus_tag="ON396_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114370.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
BASE COUNT         2573 a         1445 c         1525 g         2105 t
ORIGIN      
        1 ttttattgag aggagggatg aaaatgatta agaaaactca aaataaaatc caacgtatat
       61 gtcaaaaaca ttttgtttct agactcaaag ctgtatctaa aagaaaatac ttacatccca
      121 ttatgatttc gatcatcttg atttttactt tcttagggag cttatcaagt atttatgtaa
      181 acgtttatac agaaacgtca aattttgcta accaaacgac agaggagaca aatagtccac
      241 cctattctga aatacagtcc atagaaagca ccaatcagtc tagcaacaac gagacaacct
      301 caaccacatc ctcgactcaa ctgattgatc cgacagaaaa agaaatcaca gcagcaaaag
      361 cagcagggga gaaagcttat tcagaaacag gtagaagcca gacatttagt actgttgcgg
      421 ccactgcgaa tttcaacgga cataccatac cagatggata tacagtgata tatgccaaag
      481 atggagtcat tgttgcggtt aacggatcga tgagtgcgga taaaactgcg ttacaaggcg
      541 taaccaaagc aatcgctttt attgataacc gtccagatat ccataatctg tttattggct
      601 ccacagtaaa tggtactact atcacattcc caaaaagtat ctatatgggg gtggctaccg
      661 ctggaaatac gatcgattca gttacaccca atgcggataa aagttattct tttacaccct
      721 caacatgggg agggcttcct gccggaggag gtatgagtac tttttcaatc ggtatacagt
      781 tagcaaatga tattccattc acggggtact cgatcaaaaa tgggcagatc gttgatggta
      841 agggaaatgt ttcaaatttt ccaacctatc aagcagcaac ggttcaggtc aatgcgaatc
      901 ggacaggaac tttttcttat acagtcaatg gtggagcagc aaaaatcgga acgtacggga
      961 ctacattatc tggaatccat gtgggtgaca aagtggttat cactccaaca gcagtaacgg
     1021 gctattcttt tgcacaaaca aacggttctt tcacagccct agaaagtaac gtcgatacgg
     1081 tgacctatac aggcaaacaa acaccaatca ctattaagta tcaagactat tttggacaga
     1141 tgattgctcc ttctaaaata atgaatctta cttacggaag tgctgcacaa gacctgacga
     1201 ctaatacgcc tactatcaac ggatatacct ttacagctgt ttcagcgata gaaacaaaga
     1261 accaatcagc aatgagtgtt ttagctagtt tagatacaaa tggaaatgta atcgtaaagg
     1321 acgcaaatgg aaaacagatt tctgaagtca tcctatatta taagacaaat gcaacagtat
     1381 caatcaatgc aaatggcagt aagtactatg atggtctttc tgttgtaccg atcgttaaat
     1441 atgcctttaa tgataaaaca gaatcaacat cccttttaaa caatgatctc agtattgatc
     1501 aaatcacatg gaatccaact gatttcaaag cgatgaatga aaagggagtt gaagtaactg
     1561 cgcctactga aattggaacc tatacgtctt ggcaattgac aaaaagcgga ctagctaaac
     1621 tagctgccag aacaaactat ttatttacta tcgtgcagac atctgatgtc tatacgatta
     1681 agcaaatcag cggtgcggta gagctcagtg gcagtaagac atatgatgga aaagcaggtg
     1741 tcccgtcaat ccatgtcaag ctagcagagg gtgtcactag ttcgcttact ccagtacaag
     1801 tggcacttag cagtatggat tacacagcag atgcacaatc agcaaaaaat atggttaatg
     1861 ctggcagcta tacaattaaa ctaacgaata gcggaataga caaagtcaaa gcagcaaatc
     1921 ccaagttgtc atttactgat atagccaaca catcaggaac ttataccatc tataaagcta
     1981 acgcagtgat tactgtcgat gacgctgcct ttaattatga tgcccaaagt cactcgattt
     2041 cggttggaaa tctccacgtt accggtgttg tcttaggcga atcattggat tataccctaa
     2101 cagttaacag ccggacagat attggatcac aaatagtaac gattagttta ggcgaaggaa
     2161 gtgtaaacaa taattatacg attatcgcaa cagataccgc taagttgacg atcaatccga
     2221 ctctcttgct tccttcgaca ggtgggatag gtctactgcc ctttgtcttt ttaggactga
     2281 tctttatttt ttctggcttc ttttatttta tccatcgtaa gaaaaaagca ggtgaacagc
     2341 gatgaaaaaa acaaaaaatg tacgtgtgga tatatttgct gttctctttt ttatcctact
     2401 ggtctttttt ataggttctc cgactgtgtc agctagagaa tcacctatta catttacttt
     2461 gcataatcga atgttcgaaa gcgataagat gccaagtatc agtcaaaacg acggaataaa
     2521 tatcccggat aactttcaag gagtaaaagg gttgaacggc gttactttcg ctatatatga
     2581 tgtctcggat gaattttaca agctgcggtc agaaggaagt agtgtggaag acgcacaaag
     2641 aaaactagca caaaaaagtg atagtgaaaa aatactggca gaaaatgtga caaaaacagt
     2701 agacgaggaa gagggcattg cttcttttag tgcatcggac aaagatgagc aaggtcgtga
     2761 cgctgtttac cgatttacgg aaataaagac ttcagattaa gtaaaagaaa aatcagcacc
     2821 atttgtcgtt gtgcttcctg tgacagattc atctaatcat aaattgacga ccatccactt
     2881 gtatccaaag agcgaacaaa aaatacctgc tgcactcaca ttaacaaaaa cagtagagaa
     2941 taagcagaca gattttgcag atggggataa agtcccttat ctcatcaccg taacaattcc
     3001 tgagaacatc aacgagatag gaacctatac gatcaaagat acagctgatc ctcagttatg
     3061 gctagaacca gaaacgatcg attttttgat tggtggagac aagattcata catttcacac
     3121 acaaaaaata aaacatggtt ttgtcttatc agattctgga aaagatctta gttcgtttgc
     3181 tggaaaaaaa ttaacgatcc gttaccaaat gacattgaaa gaaaacagtg aaaaaacgac
     3241 atttgataac gatgtacgtt tgacgactga caatcaaaca gtagaaacac atcttgccat
     3301 ccaaacagga ggcaaacaat ttgtcaaagt ggatcggcaa acgaaacaaa aactagcaga
     3361 tgctcaattt ctagtcaaaa aaaatgacca atacttagcg caagaaaacg ggataaatca
     3421 ttgggtagcc aaagatgatt caagagcaac gatttttact tccagtaaag atggaacatt
     3481 ttctgtccat ggattgtctt ttggaagtta cgaactagtg gagattaaac cgcctaaagg
     3541 atatatattg agtcaatcaa ccattctctt tgaagttgaa aaaggcagtt atgatccgct
     3601 tcatcctata ccattggaaa tcatcaacga accaaaagca aatccgacga ataatggaaa
     3661 aacgccagtt cagatgcaga caaatggtcc atcaaatgaa aaaaatggag gaagctttcc
     3721 aaaaacaagc gaagagatgc ttggaggttt ctcaatttta gggttattgc tagtcctttc
     3781 aacagggaca gcttggtttt ataaaaaaca acaaaaagga aacaatagga gggaaataaa
     3841 atgaaaaaca tcaaaacaaa aatatggacg tttttaggaa ctgctatcat gctgcttcct
     3901 ttcgttctcg gcctaggaac tgcagaggtt tctgcagccg tatcacctac accagaaaat
     3961 gttacggtta atttgcataa attgaaattc acttcagcac cagaaaatca aataaacaac
     4021 ggaacggaat tgacatttcc gaatagcgaa ccgttgaacg gcgttgaatt caacgtatat
     4081 gacatcactg ctacgtacta tccaagtaaa gacacagcag ttccagcaga cgctacaccc
     4141 ttcgcaagtg taacgacttc tggtgaagga ttagctaacc tgactcttcc aggtaaatct
     4201 gatgggaaag atgctgtata cgtctttgta gaaacaccaa agcccggtgt cgaaacgtcg
     4261 ccaaatatcg tgttaagctt accctagacc ctgacaaacc tttagacacg attcacctat
     4321 atgctaaaaa tgtcatcaaa gaaagcaata tcaaaatcca gaaagctggg aacttttcag
     4381 gggtagataa gttagcaggg gcgcaattcc agatcaaaaa ttcagagaat caattcgtga
     4441 ccggttttga tgaaaacact ggtgtcgctc tctatgatcc tgctgagtca gcgttgacat
     4501 tcacaactga tactgaagga tccatcagtg tcaatggatt aatagacggc aattatactt
     4561 tagttgaaac aaaagcacca gatggttatc aattaaccaa tccacaaaaa gaaactactt
     4621 ttatagtaaa aaacggaaaa ttgcaacaaa caaccggtat catccatggc gcagatgaag
     4681 ccaatcttgg gaatccaaac ggtgtagtca atcgggaaaa tacaactacg gataacacga
     4741 ttactgtaga aaatgtccaa aactacggtg atttccactt catcaaacaa gatgtgaaca
     4801 cgcaagaaaa attagccgat gcagaatttt ctattctaag agcaaaagat ccaactgtta
     4861 aattaatgaa aaaaatagca aaccttgcct caggagaata cccatatgct tggtcagatg
     4921 aagtcacagg ttctgactgg gaagaagtga aattgatttc tgattcagaa gggaaatttt
     4981 caatcgaagg gctgaaacaa ggggcatact gccttcaaga aacaaaagca ccagaaggct
     5041 acgcattacc taagaatgaa gctgcgtaca ctgaatttac agtagatgat gatccaacaa
     5101 cttctgatga aacaacgatc gataaccaac ctaaaggaat cttgccttca actggtggta
     5161 tggggatcat cgtattcatc ttggtaggta ctgctttagt tggaggagca gtgatctatt
     5221 tcaaaaaacg ccatcaagaa acagaagctt aaactattgg aaacaaaaat catacgataa
     5281 ataaacgata gtgattcaaa tggggagaaa ggttgtgaca agtggtcggt ctcttggtat
     5341 tcgctcaaca aacggcaaaa taggaggaac tattttgccg tttgcttcgc taatactcag
     5401 gtcaaggcgc tgatttcaca acctcttcat atacatatta gaaaataggt ggacatgatg
     5461 aataaaagca gaataagaat actggacatc ttcatggcaa tcattttagt cgttgggatc
     5521 gggatctttt cttatccttt tgtagaagat tcactgaatg attttcttgc ccagcaaatg
     5581 atcatccatt atcaaaagca agcatcgaat aagaattcag aggaaataaa aaaacagcag
     5641 gaaaaaatga caaagaaaaa ccaacagttg gcagagcaaa acgtatcacc agggataggc
     5701 agtttcaacc aagcagttga tgcaaaagca ttaaaagatt tacctagtaa tgcctttttt
     5761 atggaacata tgcttggagt catcgaaatc ccaaaaatca atgtcagctt acctatattc
     5821 gatcagacaa cggaaatatt tttgcaaaaa ggaacgtcat tattggaaga aagttcttat
     5881 ccgactggcg ggaaaaacac ccatgccgtt ctttctgggc atcgtggact cccagaagca
     5941 aaattattta cagatttgcc taagttgaaa aaaggagacc agtttttcat ccagatcaat
     6001 ggaaagacat tggcgtatca agtagaaaaa atccaagtgg tcttacctga tgaagtagat
     6061 tctttgggta tccaaaaagg cagagatctc gtgactttgc ttacctgtac tccttatatg
     6121 gtcaatacac atcgactgct tgtgacgggg catcgtattc cttatcaagc aaaagaggca
     6181 aaaaaagcga tccaaggaat tgatcaatgg aaaaaatgga aattttttat ttggtttatc
     6241 ggtatactac ttggatcaat cggcttagtt tggctactca tcgcttattt agattctttg
     6301 gccatcgcta agcgaaacta tcctttatct ttttatgtaa agaacacaaa cggacgacca
     6361 atcgaaggaa tggtcttttc tgtaaaaacg ctcaacggca aacactatat tactagagaa
     6421 aaagttccat ttgtgaaagc ttctgatgaa tacggactag tgagattttc tgatttaaaa
     6481 ggcaggaact atcgcttgca acatgaggag ctcctgctta aaatccatgt aaaacataaa
     6541 cattcgaaac agttttcaat gaaattgaaa aaaggaagat ataaactgcg aaaagaaaaa
     6601 gaggtgtact atttgattga gaaagaatga atgaagatgt ccaaaaaagc agagggaaca
     6661 gactaaatga aaaaagagag aagctgacga aatggacgtg taaactgtta tttttggttt
     6721 actatctgga acgccatctt ctattttttt atgtttcaga agaattttat tattatagta
     6781 taacttttga attagtttcc ataacatttg gagcaagggg ttaaaccacg agcttgagct
     6841 tcgcttaaag tggcaggggt atacgtacca tttccacatt tatgtgtatg gtattttgaa
     6901 ccggtcggtg tgacatagac agtttgttca ttattttgct caggagctgc ggcttgtgcc
     6961 tgctgagagg cctgttcagc tgctgattgc tgtcgcgcct gttctgcttg ttgctgtgcg
     7021 attgcttctt gcctcgcctg ttctgcttgt tgctgtgcga ttgcttcttg ttgcgcttgt
     7081 tctgcttgtt gtcgtgcaat ttcttcttgc tttttccgtt cttcttctgc ttcttttgct
     7141 ttgattgtgg aatcaattgc agcaagacgg cttgtcaact cttgtttgtt ttcaggtagc
     7201 gcttggatag ctgctgaagc agttgcatag ttttcttttg taggatttgc ttcagcttgt
     7261 gacaaagcag attccgcttc tgtcactttt tttctagtgc agctttttcc gctttttgtt
     7321 tctcttcttt tttcttttca atttgttctt gttctttcga tgaatctgcc gtactttcgg
     7381 agatcgtggt actgctgctg gaagcctgat ctgaacttgt gctttgagta gtagacgtag
     7441 tagcaccaca cccactcaac aaacttcctg cgaccaaaaa caatgcaact aattttttcg
     7501 ctaaatttcc tccttaagta aaaaacaacc gttttcaata aatgtccttt tcaattgttt
     7561 acttacttgt tataataatt gtgttatgaa tagtgattga ggaaacttaa tctaatcccg
     7621 tgttgataca cgggattttt tgctatag
//
LOCUS       DAJSKS010000126         7600 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJSKS010000126 DAJSKS010000000
VERSION     DAJSKS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.130"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(942..1355)
                     /locus_tag="ON396_002311"
     CDS             complement(942..1355)
                     /locus_tag="ON396_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCR3114371.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="ON396_002312"
     CDS             complement(1811..2296)
                     /locus_tag="ON396_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114372.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2650)
                     /locus_tag="ON396_002313"
     CDS             complement(2435..2650)
                     /locus_tag="ON396_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HCR3114373.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="ON396_002314"
     CDS             2874..3674
                     /locus_tag="ON396_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3114374.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="ON396_002315"
     CDS             3693..5561
                     /locus_tag="ON396_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR3114375.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="ON396_002316"
     CDS             5554..6045
                     /locus_tag="ON396_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR3114376.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /gene="srlA"
                     /locus_tag="ON396_002317"
     CDS             6033..6587
                     /gene="srlA"
                     /locus_tag="ON396_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR3114377.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..7600
                     /locus_tag="ON396_002318"
     CDS             6605..7600
                     /locus_tag="ON396_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR3114378.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1443 g         2236 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gtttccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctga
//
LOCUS       DAJSKS010000127         7489 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJSKS010000127 DAJSKS010000000
VERSION     DAJSKS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.131"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            301..774
                     /locus_tag="ON396_002319"
     CDS             301..774
                     /locus_tag="ON396_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCR3114379.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            771..2903
                     /gene="feoB"
                     /locus_tag="ON396_002320"
     CDS             771..2903
                     /gene="feoB"
                     /locus_tag="ON396_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCR3114380.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2905..3039
                     /locus_tag="ON396_002321"
     CDS             2905..3039
                     /locus_tag="ON396_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCR3114381.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            3133..3777
                     /locus_tag="ON396_002322"
     CDS             3133..3777
                     /locus_tag="ON396_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3114382.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            3964..5157
                     /locus_tag="ON396_002323"
     CDS             3964..5157
                     /locus_tag="ON396_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3114383.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            5150..6877
                     /locus_tag="ON396_002324"
     CDS             5150..6877
                     /locus_tag="ON396_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HCR3114384.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            7271..7468
                     /locus_tag="ON396_002325"
     CDS             7271..7468
                     /locus_tag="ON396_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HCR3114385.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
BASE COUNT         2445 a         1279 c         1658 g         2107 t
ORIGIN      
        1 aactgtggaa tactaagttt tgtgtttttg aaaaaagagc tgaaaacaag tatcgtgtgg
       61 gaaaatagca aatttaatag agaaaaaatt gtggaaaact atgaagaaat atttttttct
      121 catttttatc tatcaatact aaagattcgc tcataatatc acaagtataa ttgacattct
      181 caattaccga gagtattcta tgtgagaatg attctcattt atttgcgtaa cacgtaagaa
      241 aattagaaca tataatgaat gattttaata catgatttat ttcacaagag aggaatcaat
      301 atgcagacct tagcgcaggc aaagcagaat ttcgtttatc aagtcgaacg aatcgaaaca
      361 gaagaaaaaa caaaacgcca tttgcaaaat atcgggatcg ttgccggaag taaaatcgtc
      421 gtcgtcaatt tatcaggcaa caatgccatt ttactggtaa aaagtcatcg agtggcagta
      481 agtcgggaaa tactaggtca aatctttgtc aataaaaaga ctgcttcctc tgaaagctgg
      541 acttctcttg atcaattaaa agcaggagag actgggatcg tcgtagcaat ccacggacaa
      601 ggcgcagtga agcgacggtt aatggatatg ggattgacgc gaaatgtaaa aatagttatc
      661 cgaaaactag cccctctggg agatccaatc gaactgacag tgcgagggta tgaactgacg
      721 ttacgaaagt cagaggccga actaatcttg atacaaaggg aggaaaaaga atgaatatcg
      781 cgttagtcgg aaatccaaac agcgggaaaa ctagcttgtt caatctcttg actggttcga
      841 atcaatatgt agggaattgg ccgggcgtaa cagtagaaaa aaaagaagga tcgttgaaaa
      901 aaaatccgaa cattcatatt caggacctac ccggcatcta ttctttgtct ccttatacac
      961 cagaagaagt gattgcaaga gattatttgg ttcaggaaca gcctgaactg atcatcaaca
     1021 ttattgatgc taccaattta gaacgaaatc tttatttaac tacgcagtta ttggatttag
     1081 acattcctat gatcattggt ctgaatatga tggatgtcat caaaaaagaa ggaaaaaaaa
     1141 tcaatctaga aaagctttcc tatggtcttg gtgtaccagt tttaccaatc agcgtattga
     1201 aaaagcaagg aatcgaaaaa ctaatcgaaa agattcagga gtctgcaaaa aagcggcctt
     1261 ctattcctgt tccctgtaca tatgatccac gtttagaagc agctttacat gaaattgaag
     1321 atgtactagg agaacaaagc ggcaagaaac gctggtatgc aatgaaatat tttgaacgag
     1381 atcagaaggt aagagaggat tacgagatag cagtttctca gcaaaaagag atcgagcaac
     1441 tgatccagtt aacagagaaa ctattggacg acgatagtga gacgattctg gtcaatgaac
     1501 gttatgaatt tattacccaa ctatgtacac tctgtgttgt gtcaaatgat tctttccaat
     1561 tatcgatgag tgacaaaatc gatcagattg caacgaatcg ttggttagcc ttgccgatct
     1621 tcgcatttgt gatgtggctg atctattatc tggctattca aacagtagga acaatgggga
     1681 cagattggat caatgacaca ttgtttggta catggttacc agaacatgtt ggcagattgt
     1741 tagctgaatg gcaagtagcg ggatggatgc aggagctgat cttgaacggc atcatcgcag
     1801 gcgtgggagc agtgttagga tttctaccgc aattgattat attgttttta tgcttgagct
     1861 ttctggaaga ctgcggatat atggcgagga tcgcttttgt catggatcga cttttcagga
     1921 aattcggtct ttcaggaaaa tccttcattc ctatgctgat tgctacagga tgtggagtgc
     1981 caggcgtcat ggccagtcgc acgattgaaa acgagcaaga tcgtcgtttg acgatcatgg
     2041 tcacaacctt catgccttgt tctgcaaagt tgccgattat tgccttagta gcaggggcct
     2101 tttttcctca tcaatcttgg gtagctcctt ctgcctattt tcttggtatg actgcaatta
     2161 ttttctctgg aatcagcttg aaaaaaacac gtttgtttgc tggagataca gctcctttta
     2221 tcatggaatt gcccgcatat cattttcctc aatggtcagc ggtcctaaga caaacctatg
     2281 accgcagcaa gtcttttgtt aaaaaagcag gaacgattat ctttgtttct agtatcgtga
     2341 tctggttcag ctcttcttat aatttttatg ctcaacctgt ttctgaagac caaagtatct
     2401 tagccttttt cgggcgaatc ctggcagttg tttttcagcc gcttggttgg ggaaactggc
     2461 aaggaacagt cgcagcgatt acaggattgg tcgctaaaga aaatattatt ggcacatttg
     2521 gtattttgtt tggtcatgcg gaagtatccg aaaatggcag agaaatctgg caagtgctac
     2581 aacacacata tactcccgca gcagcttatt cattgttagc cttcaattta ctttgtgcgc
     2641 cttgttttgc ggcaattggg gctatccatc gggaaatgaa tgccaagaaa tggacttgga
     2701 tcgccatagg ctatcaatgc ggtttagctt atttagttag ttttgtgatt tatcagttgg
     2761 gacacttatt ggagggtgga caggtagcgt tagggacgta tcttgctatt ttactgatga
     2821 taggactcgt ttatgttttg ttccgtcagc caaaatccaa gaaccagttt tatactatat
     2881 tgtcattaga aggagaggaa taggatggcg acattcttat taagtgtgtt aatctttgga
     2941 agtgcaggcg caattgtcta tactcgttta aaatccggta agaattgtga tgactgtcaa
     3001 acagcttgcc cagtaaaaaa ggaacagatc aagcgatgat cagtcgttgc aagaggctat
     3061 tctccccttt tttgcttatt ttgctagagc atagtaagat gagggaaagt aaaaaaagaa
     3121 agaggaaaga aaatgtctgg aaagaaaaaa atcgtccgcc cgcgctacca gcagattgct
     3181 gttgatttag ccgaacgtat cgttgaaaac cgatataaag tgggtgagaa gatccatgcc
     3241 cgctcaacac ttgccagtac gtacaatgct tctcctgaga ctacccgcaa ggctatcaat
     3301 gtgctggtag atcttggaat catggaagta cggcatggaa gtggggcatt tgttgcttcg
     3361 aaggaaaaag caaaagattt cctattgcaa tatcaagatg tacaatccat ccaagataca
     3421 aaagcagaaa tcatggcaag tgtggagaag caacaagatg agctgaatca tttttctcaa
     3481 ttattggatc agctggtcaa tcaaacgaag cgaatacata agatgaatcc tttacttccg
     3541 tttgaattat ctttatcaga aaaagcaatg catctggacc gttcaattag cgagttgaat
     3601 atctggcagg cgactggcgc cacagttatc gcaatttctc ataatgaaga actgcttgtt
     3661 tcacctggcc cctatgctaa atttacggca ggggacatcg ttcattttat tggaaatgaa
     3721 tatacactac agcgtatgac aaactttttt tacccggagc atcagcttgc agaatgattg
     3781 tgtgaaatat taaaaaaata ttaacttggc taaacctttt atttacaaag atttagccaa
     3841 gtttttttca gtttggacaa gcaaatttct ttcatttttg acaaagtgac tacatgatgt
     3901 taacatagtg tggtcacttt tttcagaaag aaaaatgaat ttacataaaa aaggagaatt
     3961 tgtttggtaa aagtagaagt aaaacattta acaaagatat ttggtaaaaa gacacaagcc
     4021 gctttagata tgatgaatga tcaccagccg aaaacggaga ttttaaagaa aaccggtgca
     4081 acggttggtg tgtatgacgt gaattttgac gtaaaagaag gagaaatctt cgtgatcatg
     4141 gggctttccg ggagtgggaa atccacattg atccgtctat tgaatcgtct gattgagcca
     4201 acttcgggca gtatctatat tgatggagaa gatgtatcga agttaagcaa agaagaattg
     4261 cgagaagtcc gtcgtcataa aatcaatatg gttttccaaa acttcggtct gtttccacat
     4321 cgaacgatct tggaaaatac agaatacggg ttagaagtcc gtggtgtacc taaagaagaa
     4381 cgacaagaaa aagcagaaaa agcattagaa aattctagtt tgctttcatt taaagaccaa
     4441 tatcctagtc agttatctgg tgggatgcaa caacgggttg gcctagccag agctttagca
     4501 aatgatcctg agatcttgct gatggacgaa gcgttctcgg ctcttgatcc gctgatccgg
     4561 cgagaaatgc aagacgaatt actggacctg caagccaatg ttcaaaaaac gattattttc
     4621 attacccatg atttgaatga agctttacgg attggggaca gaatcgcctt gatgaaagac
     4681 ggcgaaatca tgcagatcgg aacaggtgaa gaaatattga ccaatccagc aaatgattat
     4741 gtacgtgaat ttgtggaaga agtcgatcgt tccaaagtgc ttacggctca aaacatcatg
     4801 gtacctgcat tgacgacgaa tatcgaatct gatggaccaa acgttgcatt gacaaggatg
     4861 cgtaatgaag aagtcagcat gctgatggct gttgacagaa agagacacct gaaagggatc
     4921 atcactgcag atcaagcgtt agaagctagg aagcaaaaac gtccattgat cgacttccta
     4981 gatgaaaatg tgacggtgat tgggaaagac atggtggtaa gtgatatttt caatattatt
     5041 tatgactcac cgactccact tgcagttgta gaggatggaa aattaaaagg tgtcgtcatc
     5101 cgaggtagtg tgattgaagc attagcagaa acaagcgagg tgagtgaaca tgaataatct
     5161 attgaactat caattaccag tggcaaactg ggtagaaaat atcacagaat ggtttacgac
     5221 aactttttct ggcctatttt catttttaca aacgattgga caggcagtga tgtcggggat
     5281 tacaaattta ctgttagtga ttccagctcc tttgtttatc ttgttattga cggtagcagc
     5341 tttctttatt tctaagaaac gtccaggact tacgttgttc acattaatcg gtctatggtt
     5401 tatttataac caaggtctat ggaatgattt gatgaatact gtcacacttg ttcttttatc
     5461 tagtgtgatc tcgatcatca ttggggtacc attagggatc ttgatggcaa aaagcagtaa
     5521 agcccaaagt attatcaaac cgatccttga ctttatgcag acaatgccag gattcgttta
     5581 tttgattcca gcagttgcat ttttcggtat cgggatggtt ccaggggtat ttgcctcagt
     5641 catctttgct ctgccaccaa ccgttcgttt tacgaattta gggattcgac aagttcctaa
     5701 agaattagta gaagcttctg attcattcgg gagcacaagt cgtcaaaaac tattcaaact
     5761 agaattgcct ttagctaaaa gtacgatcat ggcagggatc aatcaaacaa caatgctgtc
     5821 actttcaatg gtcgttattg cctctatgat cggtgcaccc ggcttaggac gcggcgtatt
     5881 atcagcattg caaagagcac aagtcgggaa tggatttgtc aacggggttg cattagttat
     5941 cttagcaatc atcattgacc gttttacaca gcatttgaac cagccaaaca ataaaaaagc
     6001 tgcaggagca acaacacaga aaagtaaaaa acgtcaagga ctgatcattg gtgctgtagt
     6061 agtagtgatc ttaggtgcga ttggtatagg gagtttttca tctgccaaag aaacaaaacg
     6121 tatcaactta tcttatgttg aatgggatac agaagttgct tcaacaaatg tagtcggtga
     6181 agtattgaaa caaatgggct atgatgtaac gatgacgcca ttagataatt caatcatgtg
     6241 gaaatctgtt tcaaatggcg agtcagatgc catggtttca gcttggctac ctaaaactca
     6301 tggttcacaa tatgcacaat ataaagatca agtcgaagac ctaggtgcca atttgacagg
     6361 agcaaaagtt ggtttagcag tgccagccta catggatgta aattcgatcg atgagttaac
     6421 cgatcaggca ggtaagaaaa tcataggaat cgagccagga gctggtgtag ttacggctgc
     6481 tgaaaacacg atccaaaaat atgacaactt gaaagactgg aaagtagaga catcttcttc
     6541 aggagcaatg accgttgcac taggtcaagc gatcaaaaaa cacgaaccaa ttgtagttac
     6601 aggatggaca cctcattgga tgtttgccaa atatgatctg aaatacttag aagatcctga
     6661 aaacggaatg ggaagcgaag agcagatcca tacgatggtt cgaaaaggat tgaaagaaga
     6721 ccagccagaa gcttacaaag tattagacaa tttccattgg tcagaaaaag atatggaaaa
     6781 agttatgttg gaaatcaaca atggaaaaga tccacagcaa gcagcaaaag actggatcaa
     6841 agagaaccaa gaattagtcg aaagctggaa aaaataaaaa gacagacacg aaataaatgg
     6901 agcttccaaa attggatcaa atatccaaat ttggaagctc catgttcgtc tctatctttt
     6961 ttgtctataa aaaaagagaa tgcgtagctc atcttcgaaa cgtccaaaga cgccgacaaa
     7021 ctattatgaa tttatgggtg cgagtcaaag agatcgatcg taggtatgaa agactaatgt
     7081 gattaaagtt tattatgtac gaagtgcatt ctcaaaaaat attaaagaat atacatagtt
     7141 tatatactta aaagtgatta aattcttatt caggaattat tttgctacgt ttcaagaagg
     7201 aattgttttt tgaaatttaa tgaatgttag aataatagtc taaaaacatt aatttagggg
     7261 tgattagatt atgaaacatt taaaaatttt gtctattaaa gagacacaac ttatctatgg
     7321 gggtaccact catagtggaa aatattatgg aaatggagtg tattgcacta aaaataaatg
     7381 tacggtcgat tgggccaagg caactacttg tattgcagga atgtctatag gtggtttttt
     7441 aggtggagca attccaggga agtgctaaaa tgaaaaaaaa tgctaagca
//
LOCUS       DAJSKS010000128         7391 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJSKS010000128 DAJSKS010000000
VERSION     DAJSKS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.132"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(26..937)
                     /locus_tag="ON396_002326"
     CDS             complement(26..937)
                     /locus_tag="ON396_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR3114386.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1314..1952)
                     /locus_tag="ON396_002327"
     CDS             complement(1314..1952)
                     /locus_tag="ON396_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3114387.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1956..2726)
                     /locus_tag="ON396_002328"
     CDS             complement(1956..2726)
                     /locus_tag="ON396_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HCR3114388.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSTYWERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            complement(3144..3560)
                     /locus_tag="ON396_002329"
     CDS             complement(3144..3560)
                     /locus_tag="ON396_002329"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114389.1"
                     /translation="MMTREKEAYERFILLFVERFNQAFHTDYATNIFEDFENSYSYSV
                     ISSTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNGFSLHQVLWCLLGKKKI
                     IITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGI"
     gene            complement(3611..3992)
                     /locus_tag="ON396_002330"
                     /pseudo
     CDS             complement(3611..3992)
                     /locus_tag="ON396_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4118..4783
                     /locus_tag="ON396_002331"
     CDS             4118..4783
                     /locus_tag="ON396_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114390.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5180..7213)
                     /gene="brxL"
                     /locus_tag="ON396_002332"
     CDS             complement(5180..7213)
                     /gene="brxL"
                     /locus_tag="ON396_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HCR3114391.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
BASE COUNT         2369 a         1436 c         1093 g         2493 t
ORIGIN      
        1 agttcatatt ttttatttat accgcttatt tactacttgc tgtactagaa tctgaagtga
       61 atgtactcaa aacattttca aagtctttgt ctttgatttt tacattagcg tctttcaata
      121 cttcgccaat tactttattc tggaaggtac tgtcacttaa ttttgtgtct gttgcaattt
      181 ctttcaattc ttttttgtat ttatccatgt cgttgccttt gttttggttt ttcaccattt
      241 taaccacata atattccgta gtataagttg aagcattggt agaagtgatc acatcagaaa
      301 cttgaccatc tttcaactta aaggctgctt ctttgacttc tgccggaacc gttgtagtag
      361 tggaatcaaa tttcactttt ccgccatctt cttttgtcgt agtatcagtt gacttgtctt
      421 tggctaattt agagaaatcg tctcccttat cagcagcttt cttcacatct tttgcatcat
      481 cttcacttgc tacttggata atttgagctt ccacttctgg atggaaagat tcccaagccg
      541 tttttaaatc ttcatcggtc aatttgatat gtttctttag accttcttga aaggctagac
      601 tttgtttgat actgtctttg aatgtttgtt ctgtgtaacc tgcagatttc aattgtgaat
      661 caaatgaatc acccaattgt ttctttgttt gatcatattg ggaatcgatt tctttggttg
      721 atacgtcatc tccatattta tcttcaaata cttgataaac gatcaactgt tgcacagcgg
      781 aatctgctcc ttgcattttt ttcaagctat ccattacttc ttgttcgctg attgtaccgc
      841 ctttataact aacggctgtt ttttcaccgg cattcgtaca gcctgacaat aagccagtgc
      901 ctagtacgat ggcaagtgct aaaaatcttt ttttcattta tcaattctcc tacttttcgt
      961 tttttgcgaa ttgctaaaaa caaaggtgtt caagatgaaa atcatcaaga acacctttac
     1021 taatctgctc gatacgtcct gaatagatat aaggctacac aatccagaaa gttcgctcag
     1081 cagaataaat gtcaagtagc acatcacacc ttaatatact acttcttttg ttaccgtttt
     1141 tcaagtctat cttgcgttcc gtatttaacg taactaattc ttagaacaaa tgttatcgat
     1201 tttgtttttc ttcttccgct gttgggatat tcaaatcaga cacttttatg ttcgattttc
     1261 cagacatttt tttgttgttt ttattagaaa aagtttgttt tttcatttgt tttctaaagc
     1321 atgatcttct tgtttcctat tttggtcaga tatggatcat gcgtgaccac gataactaac
     1381 gtaccttggg aagcctgttg cgataagatt tcaacgactt gctccttatt tcgtttatct
     1441 aatgatccag ttggttcatc ggctaaaatg attttgctat tcttcaataa gactctcgca
     1501 caggcgattc tttgcttctc accaccagat aatgtgtaga taggcgtact tagcttcttg
     1561 tttatattta cttttttcaa tgcctcatac tttgctgttc gcttatctga aacatttaaa
     1621 ttcgaaaact tcaatccaat atctaaatta aaatcgattg tttcatattc aatcaaccca
     1681 aaatcttgaa aaagaaaaga gagggcattt ctttttatca agcgcttctc tcttccagtt
     1741 aaatgttttg tttcttgatt aaataaataa aattgccctg aactgaattg atcattcaat
     1801 cctaatatat tcaataaagt acttttccct gctccgctct ctccaactac agtcacaaaa
     1861 tccccagcat ccgcagtgaa acgatccaaa tgaagaatcg ttttgtttcg aatgattttt
     1921 ttcgcacctt ttatcactat caacggctcc aattgttaac ctccattcat cagcaataac
     1981 tgacttttct tttctgtaga caaaataaaa acaactgaaa gaataaaatc aatgaagcta
     2041 aagaaaaaaa tattaaaaag atatttaggc tctctttcta taagaaaaag gaagattaca
     2101 cttgtcgttc cccatttgat gaggttcatc atgaagaaat ctttatacgt ttcaaagagc
     2161 gaataaccac ttgaacgaaa gatagcgatt ttccgacaat tgtagacaaa atatatgcat
     2221 aacatgacat agctcaacaa gaagaaagaa aagaaagtga gtaaaaatat ctgtatcaag
     2281 taatgaaatg atctgcttaa cactttgaca gctatctctt cagtctgagt atacggaaat
     2341 gataactgca agttatcacg taactgactt tcttcaatca gctcaaataa cctttctttc
     2401 gacaaagagc ctatcttgat ctttaacggt ggatagattt ctccgctgag aatatttctt
     2461 tcccaatacg tactgttttt caatgtcagt atttcaacaa ccggataatg ttcgatccag
     2521 gggatagaag gaataaatgt ataaatgggt tgattgtttt ttataaatag aaatttcggt
     2581 tctttttcac tgatttcttg ataatattct gttatttgcg ccaggtcatt ttttaaatct
     2641 gtatgtctat atcgttcagg gatcaatata acagggtgcg tttctttttc ggttatttgg
     2701 atcaaccttc cagagacatc aatcaaagga aatttcttaa gataattcag atttattttt
     2761 atccctctgc caaactgttc attttcttca ttcaagtaat ctgatacatt gactaatata
     2821 cttccgtttt tattcaaagt ttcatataat tcctgatcgg ctgctgatgc aaaacgattg
     2881 gaataaataa aatccacgtg gtttttcccg atggttactg gataaaatac atgatagtcc
     2941 tcaccagcga tttcttttgg tgtttcatgg gaaacagcta actgtgaaga agaaaaaacg
     3001 atagttgcta attcgacatt ggcaacggtc aaaagaagag aaaacaacaa tccaaatgta
     3061 aaaagaatgt attggatata gttgaacgct tctcttaacg aagcgatttt ttcaactaat
     3121 aaaagagaaa tcaaagagat accctatatt ccgctgaaca agaaaacgta catctggaac
     3181 aaataaagaa attgctcctt ataatacata aaaataaaac caccaatgat tcctgcaatt
     3241 atgaatgtaa taatgatttt ctttttcccc aataaacacc acaatacttg atgtaatgaa
     3301 aagccattat tcaacaataa actgatttct ttattcatca ataagaaaaa ggatgataac
     3361 aaaaggaata aaagaatcaa tccaatagtt aaatacactc gataattata tacgtctgta
     3421 cttgaaataa ccgaatacga atagctattt tcaaagtctt caaaaatatt agtggcataa
     3481 tcagtgtgaa atgcttgatt gaatcgttcc acaaatagta aaatgaaccg ttcataggct
     3541 tctttttctc tcgtcatcaa gaaaaattca ccttcaaaat cttttatttt gatcgattct
     3601 tcaatggacg ttacgcgaaa ggaggtatta gaatagggta ccttctcgtg agaagatttg
     3661 acatcccctt gataatacaa tgtcatttca agctttggct gtatcttgaa gttccaagat
     3721 gtatctactt cattggattc atttactgtt tttttcatat aagaaagctc agtttctgat
     3781 gagacatttt caaataccat gattagctgc tcatattctt cttgcccaat aaaatgttgt
     3841 ggcaatcgaa tcggagtata agtatctgtt tgatggaaaa attcatttgt ccgatagagt
     3901 acctgtttat ttataataac caaggcaaaa ccactcatga taccggtcac tatgattgtg
     3961 agtaaaactg tgaacttttt tttgattatc accactctcc cttacttaaa tgattctttg
     4021 ataaaatcat accgtttttc atcaaaaaaa cgtatctatc aaatgtatag gtcatgtaga
     4081 ataaatctat atactgtaga atcgaaagga gaagaagatg aacatcggag aaaaaatacg
     4141 aacttaccgc agaaattgta agcttacgca aaaagaatta ggcgaaaaaa tcggtgtttc
     4201 cgataaaaca atctctagtt gggaaaattc tcgtacaatg ccagaccttg aaatgctgtc
     4261 acttttacat cagactttag gtctgccgat tgattttcct tctattgaat cagctaccga
     4321 atcaatagaa ccaacattgc cggatgaacc tcttgttatc aagaaatctt attcatcaaa
     4381 acaacccttc tcactcaaaa agacactatc tttctttctg ctcgtctttt gtttggtcta
     4441 tctttttctt atcaattatc aatataaaaa tgaaacggtt gatcgattca atcctttttt
     4501 gaaaccacaa atcggctata ccacactccc tgtaggatac gtggaaaatt ccatcgaaca
     4561 ttgggtatgt aatgatatct ttgttcatgg tacatggctg tcttttcacg ctggagaggt
     4621 agaaaaaggt aagaatcatg ctgtaatcaa acacaaagga aaacacgtca tgaacgttcg
     4681 cctgctcact tgggaagcca ttccctcaga ttaccaagaa atcctcatcc aagactatga
     4741 cgatgcctta aaaagaagcc ctcaggagcc agataggcat taagtaggag atgaacgtag
     4801 agatagaaaa aatcaatgca catgatctta tcaaaaaaaa acaggggtac aaattaattg
     4861 aaaaagcttt gagatgaaag ttcctaaata aaaaagttca gaatgatagg taaaaaaatg
     4921 actaactatt ctagtattag gctatttgta ttattcttct gttattttta tatcaaaaaa
     4981 tccagaaata actaaatata ggtatttctg gcttatagta taatatttac gccacagccc
     5041 ccaatgattc tttttggaat catgatcaag gacggacata cacaaaaata ttttgcccat
     5101 tcgatccgaa tagattaacg actaatctag taagtaatat agattggtac gtagtcaaac
     5161 cagcaaatgg aaaagttgtc tattccaccc caagggcttt aaagacagca tcttccggcg
     5221 tttgataaaa aatcaaatta aaggtaccca ttaattctgg gggtacagtt ccaatatcag
     5281 cagcgcttat cataggtaca agtacttttt tcgctccggc atcttttgct acttgtaaac
     5341 agttagctaa attatctacc ttaatcatag aaccaccaat tgtaatatcc cctaaaataa
     5401 ctaaacttgg taatacaggt ttatttaagg aaactgagca taaagctatt atagttgata
     5461 acgataatgt ttttgtcata ccaatgccat tcatatcttg aacatgaacg acaaaatctt
     5521 ttgtcgtcaa actaatagac tgactaacac ttccataatt agctcttaaa tatcggtatg
     5581 ccgaatccat cgcttctctt gctccagcat ctgaattgat cccagtacgt tcaaactttc
     5641 catttcccga agtcatttgc gtttccaatt tataggtacc taattgccca gttttgccat
     5701 aagataccgt gtacaaatgc cctggcttcc ctaacccttc tggaatcaaa gttcctccac
     5761 cttgttcagg aacagaaaca tactcttctc gctcttcttc taaatcaatg tatgaaaaat
     5821 tgacatcata aaattccatt ccaccaattt tctttaattg ttcttttaca cgacgacgca
     5881 tttcaagagc aaatcttagt atttcttcga catcactttt ttcaaatcta ccatcaggat
     5941 acaacagttt catcatccca gatacaatcc gacgaactgc aatcgtatcc cgctgattta
     6001 attgagaacc aagtttgaaa tatttatcaa aactatcgcc aaaactaact tttcgaagct
     6061 cacgcatgac ttcagaaaaa taatctgtaa tgaagccata gtcatttgta aaaaaatctg
     6121 gtcgatattt aggaatttcc caacctggta aataacaatg catccgatcc aaaaaggctg
     6181 aatccgtccc cataacctcc ggaaatggct caaataaatt cgaagttttc aatattacat
     6241 caacgctttg attaatattt cctacaaaag ccattgatgc tgaagcattc ttttcttctt
     6301 tgcctctagc aaaagatcct gaagccatgt aatctttcat gatttgaacg ccatcattat
     6361 ccttaaattt aatcccagca acttcgtcaa aagcaacaca gtcccacata cctacaagtc
     6421 ccattgtacg actagccata ttatagaaaa ggtttgctac tgttgtttgt ccacctgaaa
     6481 caaggattga gtttggtgaa atttctttat atacatgcga tttacctgta ccacgtggtc
     6541 ctagttcaca gagattaaaa ttattttcaa ttaatggaat aagtcttgct aaatgaagcc
     6601 attttaccct tttattaaat tgtgtaggtt ccataccaat ggaccgtaga agtaagtcaa
     6661 tccattcatt tgtagtaaac tccttgcgag ccatcttgta agcctcaatg tctaaaccag
     6721 gcatttggat tggatcaacc ttttttgcaa taaaagggct attccaacga tcattctcat
     6781 cataccaata ttccatcgaa acaatacacc agatcccccc acctaataat cgatcaaatt
     6841 ttttcggata ctcttcacta atttgaacat tttttaatcc caggttagag aattctgctt
     6901 cataacaatc tttcttttca tttaaatgta cagttacttt atcaataact gtataaccac
     6961 ccaattcacg aatttttgct tttacttttt ctgcttcatc aggacgcaca tagtttcgag
     7021 caagaatatt tttaacattt tccacacctt tttcaaccaa tgtttcatcc aatgacgaag
     7081 catacatacc aagtaaatat tccaatacat aaacaggtac attcgcacct tctttaattt
     7141 tctttgttaa atcttttctg acaatctttc cgggaaagaa ttcagataac tttaaaattt
     7201 cctgttgttc cactagaatt cctccatgtg ttcagtcatt ataatagcaa ttaaagtata
     7261 tcatacttaa ccagtaggct tgataataca aaagaaaaag tatccacaaa aaattgtgga
     7321 tacctcgttt ttatatatca aaatcaaatg attctgcaaa aggattatct ataatgaatt
     7381 ttattcgctc g
//
LOCUS       DAJSKS010000129         7103 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJSKS010000129 DAJSKS010000000
VERSION     DAJSKS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.133"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..337)
                     /locus_tag="ON396_002333"
     CDS             complement(<1..337)
                     /locus_tag="ON396_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114392.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEF"
     gene            complement(545..1471)
                     /gene="glsA"
                     /locus_tag="ON396_002334"
     CDS             complement(545..1471)
                     /gene="glsA"
                     /locus_tag="ON396_002334"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR3114393.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1494..2705)
                     /locus_tag="ON396_002335"
                     /pseudo
     CDS             complement(1494..2705)
                     /locus_tag="ON396_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(2740..4353)
                     /locus_tag="ON396_002336"
     CDS             complement(2740..4353)
                     /locus_tag="ON396_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3114394.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            complement(4465..4674)
                     /locus_tag="ON396_002337"
     CDS             complement(4465..4674)
                     /locus_tag="ON396_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HCR3114395.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            complement(5663..6877)
                     /locus_tag="ON396_002338"
     CDS             complement(5663..6877)
                     /locus_tag="ON396_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR3114396.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(7005..>7103)
                     /locus_tag="ON396_002339"
     CDS             complement(7005..>7103)
                     /locus_tag="ON396_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3114397.1"
                     /translation="IKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
BASE COUNT         2010 a         1525 c         1168 g         2400 t
ORIGIN      
        1 taaattcaag atcagccagt tctgtgctcg tgaatatacc attaaaataa taacttaaaa
       61 actttaaaaa ataagattga ccgtacagaa actgtgtgag ctcagaaaga gcaataccat
      121 gtttgaatac gagtttatac ttatgcttct ctcttacttg ttcttttata caatattcta
      181 gacactcggc tagactttct agatcgtgaa ggatcgttgg tgtagtcacc cttagagaaa
      241 tagctgtttg ttcgatgtct atttcttgga attcaaataa ttgttcacac agatacactt
      301 ttctaaatat atttcgctcg atgtaatttt ccagcattcc tgatacacgc ctccagtttt
      361 tcttatgatt ttatttaatc atatctgtaa atcgtatttt tgtttccttt ttatttagaa
      421 aagccctttc aaaaaccaat aaaaaaagaa actgggacaa aagtcaaaca agctcacaat
      481 ttcagcctca ttgtaggtag ctttgagccg tttggacttt taccccagcc tctttcaaga
      541 atggttattc aaagatgtca gcgtctagtt ctttgactac atctttcaac aattggatac
      601 cagctgcgct gttaccaaat ggatctagtg ccgggctgaa gacacctatg ccgtaacgat
      661 ttggcacagc tgccatcaac ccgccgccta caccgctttt tgcaggtaca ccaacatgga
      721 cggaaaactc tcctgattca tcatacaaac cagcagttgt cataattgat ttgacgattg
      781 tagcactttc ttcagtaatg atttgtttgc cattccaagg agcaattcct ttatttgcca
      841 gcacagcagc taattttgct atgcctgtag cggtgacatt gagggagcat tgtctgaaat
      901 aagcatcgag tatctccggt acgtctcctt cgatcatttg attacctttc atatagtaag
      961 caagagagcg attgatatca cctgttcttg attcagaatg atagatttct tcattcaatg
     1021 tgatattagg atcatcacag atctctttca taaatgacaa gattcgctca aatttttcat
     1081 ctgcatcttt accggcaatc aacgaggttg ttgcaatcgc tcctgcatta acaaaaggat
     1141 tcattggatg tttgcgatga ttgatttcca tattcatgat agaattgaag gcaaaaccgg
     1201 ttggttccgt gttgattgtt tgaaagactt tttctattcc gttgtctttg attgctaata
     1261 acaatacggg aacttttgac atgctttcga tggcaaagcg cacatcggcg tctcctccct
     1321 caatcagttg ttttgtatcc aaatcataaa tcgccatacc taattgtttg ggatccactt
     1381 cagctaatgc tggaatgtaa tccgccactt gtcctgtttg ataatagtgg aaattttttc
     1441 caatcaattg ttctaattct tttacttcca tttgaataac cagctttcat tttttatttt
     1501 tctgcatgct ggaatttatg acggcatgcg tagatcgtaa atggaataac taagacgata
     1561 agataagaaa caatcagaat cgtttcataa atcccatgct gtccagctgc gatactagca
     1621 ggtggaacaa atgagataca aaaagcaaaa agtgaagtca aaagacctaa acaagcaacg
     1681 attactttta caacttttcc gcctggaacc tgataggctc tttcaagact gtcatcttta
     1741 aaaaccagct tcaaatagcc cgcgaaaaac ataaagtaca acatcaggta gattacaaca
     1801 gtcaatgaca tcgctgcaag aaatgaaaga ttattccccc cgccgcttaa tgtaaagacg
     1861 gctgcccaga ttgttgcaac gatcccattt gcgatcatca aatgagcagg aacaccgtgt
     1921 gtgttttctt ttgcaaaata ttcaggaagt aatccttctt ctgctgctac gtgaagtgct
     1981 tttgaaggag aggttaccca agaagcaatt tctccgatga cacctaaaca aacaagaatc
     2041 gcaagaattt tcgcaatcca tgcaagattt ggatcaatgt gtcttaatag gaattctaac
     2101 gattgaatca cccctgtatt aagagacaaa ccatgttgag gtactgtagt agcgacggtc
     2161 aatccgccaa aagtatctaa aatgattgcc agaataacca atacaaacat tgctaatgga
     2221 taatcctttt tagggttttc catttcattt gcatgactgg cagatgtttc tacgcccata
     2281 tatgctaaaa taaaggaaac aaaaacaacc agcgtaggca atttcgaaaa atcaggaatc
     2341 aatgttttca tagacagtat cgtttgtagt ggagcgcctg aagcgacata atggatccct
     2401 aagccaaaca agactaatgc tggcaacaag ataccagcta caaaaccgat ccgagcaatc
     2461 aatgaagtgt atttggttcc ttttaactgt gagagtaaaa tagcccagaa aataatcaat
     2521 aggattgcca atttcagata tttattgttt tgtatagcac caatacccgt tgcataggat
     2581 aatgcaccag ttaagaaata cagcatggtc aagtagccaa ctgttatctc aaaccattgg
     2641 aaaaagattg cggcaaaacc gaatcgtttt cccaatgtgc gactgaccca agtatagaca
     2701 ccgccggttt cccttagtta aaaatattac tcttgttgat tactcaactc ttccaataaa
     2761 tcggtaatat atctcaattt cctgtattct ttcgttatcc tcattcgttg ttgcttcatg
     2821 tatgaggatt ttttctatca tggcatttaa taatggtgct gtcagttcct ttggtacgga
     2881 atattccttg attaactcaa tccacttttc agcacctatc atatcctgtt ccattttact
     2941 tagttcttct cttaggcttg ttatctgctg ttccagttct atctgttctt tttggtactt
     3001 gctggacaac atcacaaaat ttcgctctgt tatcttctca caggctctat cttcatacat
     3061 tttcgcaaat aaacggtcaa tctcattttg tctgttctcg gctttcttca atgtacttgc
     3121 ctttttcttc ttttcccgta ttctctctgc attgccagct ttctgtatct tgttcaatac
     3181 cttttcttcg tcctgctgta ctgccttagc ccaatactgc aagcgttcca atacggcttg
     3241 atacagcaca tcataacgaa tgtagtgcat agaacaatta cctttgccaa actgcccgta
     3301 gtaactgcaa gcataataac tgtaaggcgt tttatttgtc ttattcgttc caaatctcat
     3361 agaccaacca caatccgcac acttgacaag ccctgcaaaa atcggcgttg ccttttcctt
     3421 tgtctgtctg cgtcttgatt ttatctgctc ctgcacacga tagaacactt ccttgtcaat
     3481 aatcggctcg tgagtgtttt ctactcgaaa ccattcactt tcgggcttac gcactttctt
     3541 tttgctcttg aacgataccg tagactgcat attgtgaaca ctgtttccga tataaacctc
     3601 acttttcaat atcgccttga catgagcaat cgtccactca taacgcttac tttcgggttt
     3661 tccctcaaag atatgtgcaa aagtaccgta tcttgtaaaa tttagccatg acgctgtcgg
     3721 aactttttct gctcttaact gctttgtgat ttttgcactt ccgtaacctt gataggctag
     3781 ggaaaagatt ttttcaataa tccattttgt ttcatcatca accaacagtt ttcctttgat
     3841 gtcagggtgt ttcttatatc ctaacggagc ataagcaccg taatgagccc cctccgcaaa
     3901 ctttgtctta aatgctgatt tgactttacg gcttgtatct cttgccaccc attcattgat
     3961 gatgttctta aatggtgcaa tctcactttc gcctttttcg ctgtctacac cgtcatcaat
     4021 cgctatgtag cggacattat gactagggaa atacaattct gtatattgtc ctgtcataat
     4081 ataatttctg ccaagacggg aaaggtcttt cgttacaaca cagttgattt ttcctgcctc
     4141 tatatcctca atcattcttt gaaaactcgg tctgtcaaaa tttgttcccg accagccgtc
     4201 atcaatatat tcatcaatga cattcaaatg atgttctttt gcatataaac gcaacatact
     4261 tctctgtgtg gtaatgctgg aactttcgcc ctgtaattca tcatctcggc ttaacctaag
     4321 ataaagtgca gtattgtaaa tctgttgttt cattgtcagt tatcctcctt tataactaac
     4381 ccgtgtttac aatacctgct tgcaagtaaa gtataacacg ggtacttttt gtcattcaat
     4441 ctatttctta caactgcacc gattttatgc gattgtctgc tttgcttttt ccagcatgac
     4501 atcagttaga agttcttcca ttgttttgcc cttttcggaa aaatgctctt ttactacaat
     4561 ctttgtcttt cctctgttgc caatgccaca cttgccattt ttcttttgaa ttgtttttac
     4621 agcattatca ataacacata ccccctttct gtaaaatctt tctaagcaat tcaacggctt
     4681 ttgtcatagc ccacaggagt tccacctctg catagttctt atgtagccgt acccattgcg
     4741 tgcgacgctc ttaatgctca agctgtggct gtacgggagt atcattatct gacagtacag
     4801 gtcatggcga taaaagagga caaatctttt acaagaacac aaataggttt tcgctcgctc
     4861 ttgcagggga aaggtcgtgg cgtatttgtt tcaacggcta gctgtctgtc aaacgtattg
     4921 aatagcttta ttcagttgtc aaagaacgat tgaaagaaaa aaatcgtctt tcctatatat
     4981 cgcgttggaa aagaggagaa acgtaaccaa aatccaaaac tttttccatt attagtacaa
     5041 atggaacggt tcattttggc aatcaacggt tgattttctc ctttttcact actatctgta
     5101 cagcaaacgt ccatttgcta agtttaaatg gaaaaatttt aacttctttt catgcaccat
     5161 atagggatag aaaatgtcat tttgctaagt tgatgatgaa aaaagaatat tttccttttc
     5221 acaccctaac tacccaaacc ataccgttac tgccaacaat agaatgaaat attttctttg
     5281 caccatatag ggaacggaaa ccttaaattg ttaagttgag aacaaagtat tttcatcttt
     5341 tcacatcaac actcaactgc taatgcctgt ttgaatacaa aatgatgatg tttttcctct
     5401 tttcacagaa gactgtccac taatcacgaa tttccgtcat tttttcaaaa cttttttctt
     5461 ctttcacttc atatagggac agcaagcccc gatttaatga cctattttca aaaaaagttt
     5521 agatttcctt ttctcgcacc atatctgtac gcaagtagcc attttgttgc agatttaata
     5581 aaaaatttat ttttcttctt tcacttcaca tagggaatga aaacagctct ttgctgacta
     5641 aatctgaaat ttttcaatta cattaaaaaa taccgtcatt ctcttgtgga acagcggtat
     5701 ctatacgttc ttctatggtt gatttctcta attgtaataa atgtttatgc ttgtctttta
     5761 gttctgcctg ctcaatcata tctttcagta tcgttgtacg attttctctg tctaatgcct
     5821 gtaccatttt tatggttggt gcgacttgtc tttgtagcca atgcaacgct ttttgtaaag
     5881 tgtaaggctc tggctttgtg gttaagcgta tcggctctct gttttcccct acaaaccatg
     5941 cccaatcatc atttgttatc cattgacttt ttggcttgtc gtcctctctg tcaacaaagc
     6001 ggacataatg attgatgata gaaaaagcgg tgcgttcagc gtcccgatag gtcagtaaat
     6061 ctacaaccgc ataataggct cgttcgttct tcatgcgtat ttcaaaacgg ttcttaatct
     6121 ccgtatcttc aatttctgtg ccattcttga catactgctc ataatttttc tgataagcac
     6181 acatatataa ttcgctactt gttgaaccga gatataaggt ttctcctgta ttctttggta
     6241 aatcgctacc gcatttttct gtaccgctgt aatctttctg catacggaag taggacatac
     6301 attcgccagc cttgtatttt tcctttaact ttggaatatc caatattcct gctttatcat
     6361 tgatagccaa atcaagccgt ttcatcacac caccagccgt catacaatcc agcatgaagt
     6421 cataccatga gcgttcctgt gccagcagat aacattccat ttgccgacag cctttgcctt
     6481 tcagttccaa taaaacacct aaatgctcct gcatggaaca catgatattg atgtcgccaa
     6541 gtacatattt gctttcatat ccgtattttc cataatcttc ataaagcatg taattagatt
     6601 tcagttgtaa tacatcacgg ataatcgcca atgcgtccgt agtaggaaag cggacacgaa
     6661 agtaatcaat caataagaat agtggttctt gcggattgaa acgctcaatc tgtttttcta
     6721 tgatttctct taattcgtca ttcaatggct gttttccttt ttcaatgcgg ttgtaatatt
     6781 cacggctgat accacaggca acagcaagcc ttgtttgtgt tactccgtat tcctcacgct
     6841 tttgtcttag ttcttcaata aagtttttat cattcattct tcaattcctc caatcaaaaa
     6901 aggcaactac cataattgat aattgcctgt cattaaaaat ctgtatagaa aaagcagtca
     6961 atcctaagac taactgcttt gtgtgtatgg atataaaatt gcttctaatc ttgaataggt
     7021 atccaaattt caataactga attatcacta ttccaatgaa aacgataatc gtatttttca
     7081 aagtgtagga taatatcttt tat
//
LOCUS       DAJSKS010000130         7078 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJSKS010000130 DAJSKS010000000
VERSION     DAJSKS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.134"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(103..621)
                     /locus_tag="ON396_002340"
     CDS             complement(103..621)
                     /locus_tag="ON396_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR3114398.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(623..1240)
                     /locus_tag="ON396_002341"
     CDS             complement(623..1240)
                     /locus_tag="ON396_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCR3114399.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1237..2091)
                     /locus_tag="ON396_002342"
     CDS             complement(1237..2091)
                     /locus_tag="ON396_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3114400.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2088..2999)
                     /locus_tag="ON396_002343"
     CDS             complement(2088..2999)
                     /locus_tag="ON396_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR3114401.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3040..4374)
                     /locus_tag="ON396_002344"
     CDS             complement(3040..4374)
                     /locus_tag="ON396_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR3114402.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4582..5412
                     /locus_tag="ON396_002345"
     CDS             4582..5412
                     /locus_tag="ON396_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HCR3114403.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5656..5938)
                     /locus_tag="ON396_002346"
                     /pseudo
     CDS             complement(5656..5938)
                     /locus_tag="ON396_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066964.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6096..6290)
                     /locus_tag="ON396_002347"
     CDS             complement(6096..6290)
                     /locus_tag="ON396_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114404.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6795..6980)
                     /locus_tag="ON396_002348"
     CDS             complement(6795..6980)
                     /locus_tag="ON396_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114405.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT         2334 a         1409 c         1212 g         2123 t
ORIGIN      
        1 aagttattaa gacaaatgaa taaacaacta cgctttcctc gaaacaaaaa aataaagcac
       61 aaatgcgcat ttacgcactc atgctttatt cgtacaatta attcagataa cggcagcgca
      121 taggatgcca ataacacttc ctaaaatagc tcctcctaaa atgtcagtag gataatgtaa
      181 aagtaaaaaa atcctcgaat aagcgatcaa acaagcaaat aaaaaggcta atattcctgc
      241 aactggactt aattcgaaca acgcagctac tgataaaaag gctagcgctg tatgtcctga
      301 aggaaaagaa gaagtcttcg gtttacttgt gtaaaggaca agccgacgat ttttttcata
      361 aggtcttttc cgtcttgtcg tttttttgat aaaaagcaaa ccaacaaaaa aatagatcaa
      421 actgccgata agaatggcta gaccatattc tctagtatca ggtatcaaga ctaaaacagc
      481 agcaaggagc aaccaaataa aaccaccatt agagatatta ctgataaatt tcaaaaaatt
      541 gatttttcga ctatccgttt tccgttgctt ggcgaacttg ttgataatac tatcatcgat
      601 tttagcaatc aaacgcaaca tgttattcct cctttttaag aaccttttct tgcgtacgat
      661 ggataaaaag caggacttga aaataaaaca agaaactgaa acctaataaa gcaaaataga
      721 ttggtcctgc aaaccagaat gcgacaagaa ggatcaacgt gcctgccaaa tagaaaagcg
      781 cgcttttgaa atcattaaaa aacaagaaaa atgcagtaat ccaagaagct tctttttgtt
      841 ccagttgaag caagattttg taactaaaga gaagcgcgct taatagaagc aaaaacaacg
      901 tagtatacat caatgtatag ccaattcccc caccccaata gcggaaaatc tgataatcga
      961 tcaatagaag aagaaaataa acacttgcta aactatcttg ctttccataa agagagaagt
     1021 tctcacgaaa ctttttccag aatagtgaac cgagcttttt ctcttcattt tttttgtaat
     1081 gataaatcat atacagaact gttcgacttg cagttcctaa tgtgaaaaca ggcaagacgc
     1141 acaggaacca tagtagctgt aaatggataa gacgtggaat ccattcaatg atttctaaga
     1201 aataatcatt ggctttttta ccaatcgatc gattcatcat ccttttgaac ctcctgtcaa
     1261 atccatccct tcaatgaaat aacgttgtgc aaaaaagaac agcaaaatcg tcgggatcaa
     1321 gacaagtgtt gctcctgcca tcagataatt ccattgtgtc gttgcttggt tctgaaaaac
     1381 ttgcaagcca atctgtaacg tgtatttttc ttgatcttgg atatataaca gaggacctaa
     1441 gaaatcattc caagctgcgt tgaaggaatt gatcgcaatg gtaatcaagg caggtttcgt
     1501 caatggaagc atcaaatgac tccaaatata caaatgattc gctccgtcga tttcagcagc
     1561 ttcgatcaat tcattattga tcgataagta aaattgacgc atcagaaata tattgaacgc
     1621 attgccgaag aacgatggaa cgatcagggg caaataagta cctacccagc cgatcttgga
     1681 gaataataca tattgaggga tcatcgttac aaaccccggg atcatcatcg tagaaagaac
     1741 caaagcaaac atcagctttt tcccaggaaa atcaagcttc gcaaatccat aagcaataaa
     1801 agaatttgaa aggacattac cgatcactac tagcacagtg atgaataatg tatttcttgc
     1861 atagcgccaa aaaggaaagg cggcaatcgt atccagatag ttttcccata cgattttttc
     1921 tgggaaaaag gtcggaggat aacgaacgat ttcttccatt ggtttcaatg aagtaaacac
     1981 catccaaagc aatggaatga tcaaaatgat tcctattact gtcatgagga cataacttag
     2041 tatttttttt gtttgccgat tcacactggt ctttttgaaa taatccatca tttgccgtca
     2101 cctccagaat aatagaccca cttaggtgca agcttcaaat tgacaaacgt cagaaccaac
     2161 acgatcagaa acaggatcat tgacatagcc atcgcataac ccatatcaaa agctacgaac
     2221 gccttgttcc acatatgcag attataaaaa agcaaagaat ttgcaggtcc gcctgttcca
     2281 tttttcgtca tgacataagc ttcttgaaaa atttggaaag aaccgatcgt agaggtgatc
     2341 acatcgtaaa aaataatagg agtgattaat ggcaaagtaa tacttttgaa ctttccccaa
     2401 acgcctgctc catcgatttc tgctgattca tataaagatc taggaacatt ttgtaatgta
     2461 gctaagtaaa gtagcattgc tcctccagaa ctccatattt tcatcaaaat caacgcaggt
     2521 ttcgtccatt gtggatcaaa cagccaagct ggtccaggga tatggaacca gcccagaaat
     2581 gtgttgatca atcctgcaga tggagaaagc agctgcatcc ataagatgta gactgctact
     2641 cctgataaga ctgccggtaa atagaaagct gtacggaata cactgattcc tttgattttt
     2701 tgattgagta ctgtggctaa aaacacactg aaaatcgtcg tcagcggtac attgaagatg
     2761 acgaaataaa cggtgttgta taacgatttc caaaataaat catcgtgaac gaacattctt
     2821 ttgaaatttg ctagaccaat gaaattcatc ttggatgtca aattataatc ggtgaaacta
     2881 ccaataactg aaaaaagcat aggccctagc ataaatacca agaacccgat aataaaaggc
     2941 gagataaata aataggccca tcttgcttct tttttctttc ggatagataa acgcttcaaa
     3001 ttttcttgcc tcctctgtac ctagacagtc aaaaatggat tattgatttt gcttgactaa
     3061 gttttctact gctttttgtg catctgccaa tccttctttt ggtgattttg ttcctaaaag
     3121 aatctggtca atctgttcat tcactaatgt cgtgtaatcc ggagcatcta aaggaactgg
     3181 tgtgatcacc gtatttttaa agttttcgtc cgccatttga tagatcattt ttcctttatc
     3241 cgttaggttg tcattattca ctaaattctc attggctgtc ttgctcgcca tgatatcaaa
     3301 acttttttcg ccgaattttt cttgtacttc tggagaagaa agatatttga tgaattcata
     3361 agaggcctca ggatcttttg ctccgtatgg tacttccaag acgaatccgc caccccacga
     3421 ccaatggcct gaaccttctt ctttttctgg gagctgcatc acgccaaaat caaaatcatc
     3481 cggtgcattt tccatcaaac tagagtaata attgatattt tgggcacgca tggctactaa
     3541 tccggaaata aatggatcag atacaccaga cccaaattcg gcttctaatt tattgatcgt
     3601 atttttccca taataatctt gccagtcttg gatccactcc aaggcttcca ctttattttt
     3661 agtatcaatc ttgacttttt catctttgtc aaaccaacta gtcccgttat ctgcgtttag
     3721 cgcccataca tcagccccta aattccataa tggataaaaa ccgatgcgtg taaattcttt
     3781 tccttctttt ttatctagtt tacgtgctgt ttcttctaat tcatcccaag ttgtcggtag
     3841 atcttgttca ctgattccag cttctttaaa taacttttta ttataaaaaa tgacttgtgt
     3901 atctgtattg aatgggatcg cataaggatc accttcatat tctactgtat cccaaagctg
     3961 cggataaaaa tccttagaag tgtctttttc tacatatttg gagagattag ttgcttgctg
     4021 tgcttctgct ctttgggcaa cggaatttat gtcctgtaca ataacatcgg gtgcatttcc
     4081 agctgttatt gcagataaag atttagtcca gatgtcaccc catggctgat acttataatt
     4141 tacatggatc tcgttttgtg attgattgaa atcctcaatg atttcttcga tagcttcttt
     4201 tcgtgcaccg gatccccaaa aggaccagaa atcaatattt tttcttccat cacttgtttt
     4261 ttcagaagaa tttgctgcac ttgaactggc atcgcttgca ttacttgatc cgcatcctcc
     4321 taataaaaac aatgctgaac ttgccagcaa ccctgtaaac aataattttt tcatatttta
     4381 cctccaaatt ttaatgtaac cgatgccggt tttaatataa cgatatcatt ttgttctgaa
     4441 aataacaatt tataaggtat gattctaaaa aaattattaa tgataccaat actttatata
     4501 attcgcgagc gctttctttg aattttttaa tctttcgtag cagaatgaga atgtattaaa
     4561 aatagattag gaggaatttt gatgtttaga caccagaaag aattgcagtt tgaagcaaaa
     4621 ccagatcgtc cgaatccatt aatcgcgaaa tatctgcagg agctgatcgg cggccaatat
     4681 ggcgagatga gtgtagctat gcagtacctg ttccaaggct ggagctgccg aggagaagaa
     4741 aaatataaag atatgctgat ggacattgca acagaagaaa tcggccacgt ggaaatgttg
     4801 gcgacaatgg ttgctcgttt attagagggt gcgcctgttg aagatcaaga agcagcgatg
     4861 caaaaagatc caacaatcgg tgccattatc tctggaatga atccacagca cgcgatcgta
     4921 gctggcttag gacctcgtcc tgctgacagc gtgggcaatc catggagcgg tgcctatatc
     4981 attgcgagtg gcaacttgtt agcagacttt agagctaatt taaatgccga atcacaagga
     5041 agacttcaag tagcacgtat ttacgaaatg atcgatgatc gtggcgtaaa agatatgctg
     5101 tctgttctcc ttgcaagaga tagctatcat caaaatctat gggaatgtgc agtgaaagaa
     5161 ttagaagaaa aagaaggttc aatcttagtc ccaagtactt tcccgcgtga caaagaacga
     5221 aaagacattg cttatgactt ctataacttc tcagagggag accaaagtag tgaaggttct
     5281 tgggctagtg gcaaagcact ggatggcgaa ggcgaatatc aatggttgaa agaacctaag
     5341 atcgaaggcg ctgcaccaaa attagaacca gtaacaccaa aaatgtatgg gacacctcca
     5401 ttcaagaaat aaatatctac ccgtagaaac acccgttttt tgttccacac gctttgtgag
     5461 caaaactgac taaagtcatt aatcataacc acccttcaaa agggtggttt tctctagagc
     5521 tataagctca aatcaccggc cagcgcctaa agacgctggc tttcatattt tatgttcaag
     5581 ccatattgcc tttgccagtt ttgcccacct cttaaaaggt ttatgtacta ctccttagcc
     5641 cttaaaagga tcgtcttact ctcgtacact cagcttatct aacgcactat catgtttttc
     5701 ttgttcttga atgtactttt tgatggtcgc ttcattttgt cctacttttg tcacatagta
     5761 tccttccacc caaaagtgtc gatacccaaa tttatatttt agatttgcac aatctcttga
     5821 tgatctcctg aatattggct cgatattgat tataaattat ttttcttcta tactttggtg
     5881 tgaatacaat gtggtattta cacatctact ttgtacgggc taaactattt ccctttattg
     5941 ctccacccgc atagcaagtg gttttgtatt tcaatcgctc ggcgcgctca attaggtcta
     6001 aagacaataa atctaaaaac ccgctgcttc actgacagaa acaacgggtt ttcagaaaaa
     6061 aatgaaatga aaaagatctc ttacggcaat ctattttatt tttttgattt tttataaatg
     6121 gaaaatgctt caagaagtcc aagaaataaa gccgtataaa aacaagaacc tatgaaatct
     6181 ttatttatta gatattcaac agaaatcccg ataacactag ccaacaatat atttatcaga
     6241 agtgtaaaat aaggatgctt tttatagaat agaactagtg ttgttttcat agactctcct
     6301 ttcttcatat ttccaattaa tcacaaattc acggcattac tagaaaaatt aaaggggcgc
     6361 agaagtggaa aaatactctt cgactttccc tacaaaccag gccatcttgt gtttaataac
     6421 tataccaaat ctaaggtacc tctaaatcta aattccattt gtggcggtga cctccaattt
     6481 ttaactcttc tatctcccat ccagtataca taaccttgat acttcgacca cttatatatc
     6541 gaacacacaa atacctatcc attgcaggag tactacctaa cttagttgct acttgcatct
     6601 aaataacagc tgaaaagata ggacttactc ttagtaaagt atctgaacta ttaattttta
     6661 aactatcatt tttatcctca actacatgca catctgttgc aacttcactt tctgtctcat
     6721 ccagataaat gattaaggaa tcgggaattg caatatcttc agctgacgct ggaatagttg
     6781 agctaaatta gtcatcatat ctttattata aaataaccaa cagcaaaaaa tacaatgaaa
     6841 aacaaacttg gaactaatgt atcattaata aatgttgaaa aattcagcaa ctcattactg
     6901 tatgatttta gcagcccaac caacatatac cctataaaag ctaataagat ggttaggaat
     6961 cctttagaat ttttactcat tcatagacac ttccttaaat aacttttatt atagaaggta
     7021 ttgtaaataa taatattgac aaaaagtgac tcaaaataaa aaaaacctta cattatac
//
LOCUS       DAJSKS010000131         6983 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJSKS010000131 DAJSKS010000000
VERSION     DAJSKS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.135"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            182..1039
                     /locus_tag="ON396_002349"
     CDS             182..1039
                     /locus_tag="ON396_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCR3114406.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1032..1934
                     /locus_tag="ON396_002350"
     CDS             1032..1934
                     /locus_tag="ON396_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCR3114407.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1967..2317
                     /locus_tag="ON396_002351"
     CDS             1967..2317
                     /locus_tag="ON396_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR3114408.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2366..3289
                     /locus_tag="ON396_002352"
     CDS             2366..3289
                     /locus_tag="ON396_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR3114409.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3367..4260
                     /locus_tag="ON396_002353"
     CDS             3367..4260
                     /locus_tag="ON396_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3114410.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4298..4861
                     /gene="amaP"
                     /locus_tag="ON396_002354"
     CDS             4298..4861
                     /gene="amaP"
                     /locus_tag="ON396_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCR3114411.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4875..5063
                     /locus_tag="ON396_002355"
     CDS             4875..5063
                     /locus_tag="ON396_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCR3114412.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5076..5978
                     /locus_tag="ON396_002356"
     CDS             5076..5978
                     /locus_tag="ON396_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3114413.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            6010..6252
                     /locus_tag="ON396_002357"
     CDS             6010..6252
                     /locus_tag="ON396_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3114414.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6508..6879)
                     /locus_tag="ON396_002358"
     CDS             complement(6508..6879)
                     /locus_tag="ON396_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114415.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2542 a         1188 c         1391 g         1862 t
ORIGIN      
        1 tcaatacctt tgaataaaaa gccatgaaaa acgaatagtt agcgcttacc gatgcgtttt
       61 gtgataaaaa aattttaaaa tattcaatga aaacaaagca tatattttgc tttaaaacca
      121 aatgaggaat attctagatg tagaacaggt tagaaaaaga ttagaaaaag gagtgatttt
      181 gatggagaag agatacggcg tagttcctgt ggtaactgct tcagacgaaa attatgctcc
      241 ttacttgagc gtgatgatcg caactgcatt agaaaattgc aataaaacaa gacgcataaa
      301 attttatgtg attgacgatg gtctatcaga atatagtaaa gaaggattgg aagaaacggt
      361 aaataaatat tcaagtaatg caagtatcca attcctgaca gtggaaaaag atatctacga
      421 agacttccta gtcagtgacc atatcacaac taccgcttac ttaagaattt ctttaccaaa
      481 cttattagct aaagaggatt ataagaaagt cctttactta gattcagatg tgttagtact
      541 agacgatatc gtaaaactat atgatgagcc gttaaatgga aaaacaatcg gcgctattat
      601 cgatccaggc caagtgaaag ctttggaaag attaggaatc gattccgatg acttgtactt
      661 caattcagga gtgatggtga ttgacattga tcaatggaat aaaaaagaaa ttacagaaaa
      721 aactatccat tacctcagtg aaaatggcga tcgaatcatc tatcacgacc aagatgcttt
      781 gaatgctgta ctttatgaag attgggaaca acttcatcca aaatggaaca tgcagacttc
      841 gctgattttt gaacgtcatc cagcaccaaa tgaaaaatat gaacgtcagt ataaagaagg
      901 aaatgaaaaa ccttcgatcg ttcactttac aggacatgat aaaccttgga atacgttgaa
      961 agatcatccg tataccaatt tatacttaaa aaaattagca cacagtactc taacgaaagt
     1021 aggcgagatc aatgaataaa aaagaaatag cagtagtcgc aagttgtaac acaaaattcg
     1081 taccacattt agctgcatta tttgtctcag tattagataa ttgcaacccg tctaaatttg
     1141 ttcggttcta tgtgattgac gatgatatcg attttgaaag taaacagctt ttacgcttct
     1201 cagtgaaaaa cgcacgaatg aactcagatg tggaattctt gaaaatcaat aaagaattct
     1261 ttacaaatgt ggtaatcagc gatcgtattc cagaaacagc ttattatcgt attgcgattc
     1321 cggaattgtt ccgcggcaca gaagttgaac gtatcttata tatggattgc gacatgatcg
     1381 ctttacaaga tatctctaaa ttatggcgtc ttgacttcgg tgattcaatc gtagcagctg
     1441 ttgaagacgc tgggttccat caacgactgg aaaaaatgaa aatttcagct aaatcaatgc
     1501 gttatttcaa ctcaggtttg atgctgatca atgtgaaaaa atggttagat gaaaatatta
     1561 cgcaaaaagt attagatttc atcgaacata atccagaaaa attacgattc catgaccaag
     1621 atgcattaaa tgctatctta catgaccgtt ggttaccact tcatccgcgt tggaatgcac
     1681 aaggctatat catggcaaaa gcaaaaaaac atccaacagc agcaggtgaa cgtgaatacg
     1741 aagaaactcg caacaatcct tacattatcc atttttcagg acatgtgaaa ccgtggagca
     1801 aggattttga agggccaacg aagaaatatt acgaaaaata tgcagggatg acggctttcc
     1861 gttgtgtagc taaattccct aaatatccaa aatatgcaaa agttcaacca aaaacagatg
     1921 tagttgttaa ataatagaag acaacaactg aagaggggga gtaggaatgg aaattacaat
     1981 gtctaaaaat gcagtagaga cgttaattga aaaggttggg gaaaatacaa aaatcgcact
     2041 cgcgcttatc aatgattccg atccatttct aagggacaaa ggtgcttttg caaaaggcag
     2101 cttttttcaa atcattcctt ttgtttcgga atttggagaa tatgcgacta aaatcgagca
     2161 tccattattg gacatctata catctaaatt agagcaaaat tatttcggta aacgattgaa
     2221 catggacttc aacaaacagc ttgacagctt ttcattagaa aatgaaatcg cagtactcga
     2281 ttataatatc aaattaaaga actgcttctt cagctaatag tttcgagaaa gtccaaaaag
     2341 gaggtataca ttgagatact actttgtgga tgaagaaaag aatgtaatag aaacagatga
     2401 aaaaaaatat aattggctcg tacttgatag tgcaaagccc gaagagattg agcaagcaat
     2461 cgaagaattc gagcttcctg atgatatttt cgtaggaacc gaatatccgg aagaagtttc
     2521 tcggatggag cgcctttatg agacgaaact agagcatcca ttttctttgg tagtgattaa
     2581 tctagatcag tcagataatc gtatcgaaaa aaaattaacg ccaatatctt ttgtactttc
     2641 cgataatctc ttgattactt gtatcgatag aaaaaccgaa tttgtggatc ggctgctgac
     2701 aaagcatgga aatcatttaa actcgtttga aaagatcatc acgtatacat tattagatat
     2761 ttacacccat tacgtgaaag aactaagaga gatgaaaaag cgaattgacg ctttggataa
     2821 agcagctcgt aaaacaacgg aaaatgaaga actgtataaa caggcagatc ttgaaagaga
     2881 catcgtatat atcgatcata ccttaagaga tcaacgggaa acaatggatc aattatggaa
     2941 ttctgaaact ttcatcaaac gtttagacga tgaacagcta ctctatgatg tccatttgag
     3001 acagcgacaa acagaaaaaa tgatcgagat ctatcgagat ttattagaga gtattggtga
     3061 tctattcaac gggatgatgg acaataatct aaatcatatc atgaaatatc tagattcagc
     3121 tgcattaatc atttccgttc cggcgttgtt cgcagggatt tggggaatga atacaggagg
     3181 attaccgggt aaaagttctg ctctaggctt tatactcgta gtctttggct ctttgatagc
     3241 agcagtagtt ttaggttacc atttataccg gaaagattat acaaattaaa gaatctattt
     3301 aaaacaggta tcaaacaaag aaatttgttt tggcaataca atttaaaaac taggaggaat
     3361 acagcaatga cagaaccaaa attaatcgac ccgcgaaact tgtaccatac ggagaaattt
     3421 cctaaacaag atcaggatac accagcttta cagcagaata tgattcctgt tcctgattgt
     3481 ggagaagaat cctatgaagg caataatcaa ctagaaaacc gccgtgtact aatcactggt
     3541 ggagattcag ggatcggtcg tgcagcagcg attgcatttg ctagagaagg ggcagatatc
     3601 gctcttcaat tcttcccagg agaagaaaaa gatgcagagg aagtagcaaa atacgtgaaa
     3661 gttgcaggac gtaagattgt cttactacct tttgatcttc gtgacgaaca agcacctaaa
     3721 gaaatcatag ataaagccgt gagtgaactt ggcgggctag atacgatggt actaaacgca
     3781 ggacagcaaa tttcttgtca gtctattgct gagttaccac ttgagcaagt caaagacaca
     3841 tttatgataa acatcatcgc tatgtttgct ttagtgaaag aagctgttcc gcatatccca
     3901 gcaggaggag caatcgtaac aacaacttct gttcaagcat tcaatccaag cgaacattta
     3961 ttagattacg cagcaacaaa agcttcaatc gctaacttta cgatcggttt agcaaaacag
     4021 cttgcaccta aaggaatccg tgtaaatggt gtagcacctg gtccaatctg gacaccatta
     4081 caactagacg aaggacaacc ttctgattcg atccctgaat ttggtcagca aagtttacta
     4141 gaacgtgcag gtcagccagg agaattagca ccagtttatg ttttcttagc atcgaataaa
     4201 gcaagttatg ttacagccca aatttacgga gtaactggtg gagaagcgat caacttataa
     4261 gaagttgatc gtttcacccc tatgaggagg tgcgaaaatg aataaaggtg taaaaacgat
     4321 cggagtgatc ggaacattac ttttgctatc tgtgcttttg tttgcgatac tattaagtgc
     4381 aacctatcca ttgccttttc agctagagaa gtttcgattc ttttccatca ctaattcata
     4441 cgtgcaacaa tatgtatttt ggcttgccgt tgcatttgct attttgacaa ttatcacgtt
     4501 acttgttttc ctgttttatc caaaaacaat cggaaacttt ctactgaaac atggtgatgg
     4561 ggagttgacg ttggataaaa aagcaattga aggaatggtt cgttctcact tgcatgaaga
     4621 tgaattcatc cattcaccaa aagtaaatgt caaagcaacg aaaaacaaga tcgccgtcaa
     4681 tgtaaaaggg gagttgaaac gaacttcagc actcattggt aaaacaggaa cattgatgaa
     4741 agaaatcgaa aatgaagtac aacacatatt gggaacaaaa gagccagtga aagtatcggt
     4801 gaaatacaca ggctttgaag aaccagctaa acgaagcaat gaccgcaaac gagtagaata
     4861 gggggggacc aaaaatgaaa gaactatttt cgacatacaa gctccctatc atctttgggg
     4921 tacttggatt gatattagcc gttttatgga tcaccgtggg ctttttcaag accttattga
     4981 tcttgatcat ggcagcgatc ggcgtcgcta tcggttttta tctgaaacaa acgaaaatct
     5041 tagatcacta ttttaaatct taggaggaaa catccatgaa taacaataac aaaaataata
     5101 acaataaaat gaacaaccaa aaaccagtgt ctgcacatcc agacccaacg acaccaaacc
     5161 aatcaaatgg tgtgaacgat gaaaagaaca aaacagcacc agtatctgca catccagatc
     5221 cagtagtccc taacgttcaa caaggagtag aagagaagaa acatgatgca gaaaagcatg
     5281 cacctcatca tccaggaact ccagaatcta aagttccaga acaacatgat gcaatcgatc
     5341 cttctaaacc tgtggaagag caaaaacatc cttctgacca tgcgaaagca caagaaaaac
     5401 ttagttctga ccacacaaac ggacaaggaa aacttggttc tgatcataca aataaccaag
     5461 gaaagcatga ttctgaacag gaagattcaa tgcgcggtga gctgaattat gaagataaag
     5521 ttgtgcaaaa aatcatcgga atcgcaattg aacaagtaga cggtttatta tctgcaaacg
     5581 gtggtttctt ctctaatgtg gcaggaaaac ttgtgaatac agataatgta acggctggga
     5641 tcgaaacaga agtcggtaaa aaacaagtag cagttgatat ggatgtcatt gtggaatatg
     5701 gcaaagatat cgaaaaaatc tttgaagaaa tgcaagaagt aatcggcaaa gaagtaaaaa
     5761 atatgacaca tttagaagtg atcgaagtca acgctaacgt agtggacatc aaaacaaaag
     5821 aagaatttga aaaagatcaa gaaacggtgc aagacaaagt aactgacgca gcgaaaaaaa
     5881 ccggtgaatt tgcttcgaat caaacagata aagtaaaatc agcagctgga agcggcgcac
     5941 aaaaagtaaa agaaaataca gaaccacggg ttcagtaata aggaagtaaa acattaggag
     6001 gaacgaaaca tgggatttat ttggtcactt atcgtcggag gaattatcgg agcaattgca
     6061 ggagcaatca cgaataaagg ttcttcgatg gggattattg caaatgtcgt agcaggttta
     6121 gttggatcag cacttggtca agcattatta ggttcttggg gaccaagtat tgcaggaatg
     6181 gcaatcgtgc catcattgat tggtgccatc attttagtac taatcgtatc attctttgct
     6241 cgaaaactgt aaaaagactt ttaaaaagag gtcgtgaaaa agctgttctt catcaagtaa
     6301 taagaacagc caaacaaaaa tagcttctca tatttttcat ttggttgggc atattatcga
     6361 agatgcaagc ttttgaacac tgtttatacg gaataagagg ctgtgacaag acttttgtca
     6421 cagcctctta tttatgcggg caaattccca attagatttt taaaagtttt tccttaaatg
     6481 tgtctttatt gatacagaga agcttcttta ataaaacaat gcctccctaa attttcttga
     6541 gataggaaag gtgaacatac aaaataatac gaccactttg aaaacccaga tgatccacaa
     6601 atggatcgta tgatactgat gaagtaaaaa gcaatggatc agaaactcaa agagattgaa
     6661 aaagacaagg aagaaataat atctcttttt tactcttgtc tgttttagcc aataatagct
     6721 cgtaaagtaa tctagacaaa caaatagaag taaacaaagt ttcgtaagag cagcaatcgt
     6781 atcatgttgc tctgctagac gattgacgag caggaaaaat gttggtaatg gaatatagat
     6841 cacgagtaat gaaagaaaaa agtaaacaat aaatggcata ctattcctcc taattgtgtg
     6901 agttcgccct cattttaaaa taacgatatt tgtttgtgaa gctttttcgt gtcgtaacaa
     6961 atgttatcta atgtattttt aaa
//
LOCUS       DAJSKS010000132         6883 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJSKS010000132 DAJSKS010000000
VERSION     DAJSKS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.136"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..2009)
                     /locus_tag="ON396_002359"
     CDS             complement(<1..2009)
                     /locus_tag="ON396_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR3114416.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
     gene            complement(2025..3021)
                     /locus_tag="ON396_002360"
                     /pseudo
     CDS             complement(2025..3021)
                     /locus_tag="ON396_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
     gene            3240..4462
                     /gene="sstT"
                     /locus_tag="ON396_002361"
                     /pseudo
     CDS             3240..4462
                     /gene="sstT"
                     /locus_tag="ON396_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            4609..5244
                     /locus_tag="ON396_002362"
     CDS             4609..5244
                     /locus_tag="ON396_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCR3114417.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            5435..6175
                     /gene="truA"
                     /locus_tag="ON396_002363"
     CDS             5435..6175
                     /gene="truA"
                     /locus_tag="ON396_002363"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR3114418.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(6320..>6883)
                     /locus_tag="ON396_002364"
     CDS             complement(6320..>6883)
                     /locus_tag="ON396_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114419.1"
                     /translation="KNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAI
                     AENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVL
                     TTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSI
                     VTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         2181 a         1081 c         1323 g         2298 t
ORIGIN      
        1 attttttgtg tcgcccatgt tgcactgtac tggttacctg caacaagatc atattgtatc
       61 agacgataat cttctagaat ttctttttgt ttctcagtta aatttttttc ttggataatt
      121 tgactatcct ctgtaacaaa gacctgtttt ccattaaaac gattagacaa gctttctgta
      181 ggatcaattg ttaatgcagg tagatgatta gatacttctg tcaacaacgc atagtaagga
      241 gttactttga tattcgcatg ctcaagagcc atagcaccaa agttattagg agcaacaact
      301 tctttattta acgtagctgc ttgttcacga ctatacttgt tgttataaat gaaataatct
      361 gtctggtgct gttctaatcc atattttttc gaatcattcc cactatagat tgcgggtaaa
      421 tggtcaccat aaaaaacaaa ggtaatcggt ttatcgattt tgtcgatttc ttggatgaat
      481 ttttttactg ctgtatcggt atagtgaagg ccttgcatat aagtttgtaa ttcttttctt
      541 ctattaggaa tgacagcagt tccttcagca gtgtagttca attcactata gtaatcatca
      601 taaggcatat ggttttgcat agtggataat tggatgaatt gtgacgtttc tgtattgctt
      661 tgtaaagctt tcaaggtttc tttgtatgct gaatcatccg atatgcgtgg gctttgttct
      721 attttatccg tataagttaa tttatccgga ctgtctaaat aatagaacct gtcaaaacca
      781 aatttatcaa acacttgttt tctgcgataa agagcagctg taaaaggatg gatggctatt
      841 ttttcatcga atagatcggt aatatttgga cttattttct gacgctccac tagctgggta
      901 taaggtgtaa ctaacgaagc agacaggtta ctgatactca agcctgtcag tccttcccat
      961 tccatgttag ctgttccgcc accataaccg acggataaca tcaatcctcc tgttccttct
     1021 gttataatgc ttttgatgta aggaataggg tctgtaggta ctgtcagatt agggacacgt
     1081 gcaggatcac tgaagctttc acttaaattg agaacgatcg tctgattgtt taaccagtca
     1141 cttcttgttt ggttgatttc atctgccttt gtgttgtact ttttcattat tccattaatt
     1201 ttttctttcg aatagccagc aggttcttcc atgattttaa tgtcgatatt gtttaggaat
     1261 tgaatgactg gtccgttatc ttttacggcg ccttgctggt taaagaaagt cttgtttact
     1321 ttaaataaat taaacagcaa gaaagaaggc gaatttttgt gattaatgaa aaagacaccc
     1381 gaaaaacatc ctaataatac agcgatactg ataaatcgac gtttccaatg aatcttcaag
     1441 gaatactgtt tttgtagtct acgttgtaca ataatactag taatcaataa taggagaagg
     1501 atcaatccgc ccacgattaa taagactggg ctaaccatcg ataatagttc tgacaatccg
     1561 gtcagtattt ttaaatctga aggaaagaca ggctcttccc gcatttccat tttgattact
     1621 gtcgaaatag tgagcagtaa gttaatgatc aaggtagcag tcaaggcgta ccaaaaacga
     1681 ttggaaatca acaatagtaa gaagaaaaat cccataataa tgatgattgt caggatcatg
     1741 ggtgcttgtc gctgagtaaa tatttttaag gcaacctgga tagtttcttt ccaaccttct
     1801 tttggttcta aaacgaaaat ctgcacgaag gtaaagcagt aaaaaaatgc tgcaactaaa
     1861 aaataacttt tatgaacaaa aagccaatga attctttttt tccaacgctg agttaattgt
     1921 tcaatcgaat caaaagcgaa gtattcgatg aaacgatgaa tgaaacggat tttttgtata
     1981 taaaataaaa agataactag gagggacact gctaataaat agcaaataaa aaactgcccg
     2041 atatttatca gccatgcctt ccctgagttt ggaaattcac gtttcataaa tgtagatacg
     2101 cgataagaaa ctaaagggaa attacatata acttgagtga caatcaaaaa tacagcaaga
     2161 aaatttcctc ttccttttat acctaattta tcagatagca tttctaaaaa taaaaacgaa
     2221 gagaaagtat aatacattgc gaagagagaa tatggtttag aaaacctcag cgctgtatct
     2281 gtatttcctc tgatgatgag tgaaatttgt gtcatcaaaa taatcaaacc gaataaaaca
     2341 atcaagctga taaatacttg aggaagctta tagcggacct tttctttcca gttccattct
     2401 ttcaaaccat aacccattaa aaataaataa aaaatccata aaatactctg tccattctgt
     2461 aatgcaaacg ggtctttatt aaatagagta gagcagccaa ctaatccaat tgttaaaaat
     2521 aatagtagat atttaattgt tgttttcgat aatttattta aaaatccaac tgtataagga
     2581 atactacaaa aaagcaaagt acaagaaaca gcataagtaa agtaattctg actgataggt
     2641 gttaggataa gccaataatc acgaatattc aagctgccag cttgtacgta aaaaaaagat
     2701 attgcaaact agtgtggcta ttaggaatat aaaggcaaaa cgacttaaat aatgtaaaac
     2761 ttttaaacgg gcgatttttt gatattggta acccatgtaa agtgggatca aatttgcacc
     2821 aataacaacg gaagtttgta aaaatgtata gatgtagaat cgaccagggt ttgttgcata
     2881 tgtaggaata gtgactgctg aaatcaatat acgctgtaaa accatcatta tacaagcgac
     2941 aaagattgtc aggaagccat aactctcctt ctttattttt ttcaagaata aacacctcac
     3001 aaaaaaattg gacaaaaaca taagaaaacg tttcctgata agttgcccat taataacatc
     3061 ctaacttaga atatctcaaa aaacaacgtg tagacagaaa aagtacaaaa ttagcaagaa
     3121 atggttgttt gtattgttag aatttttaga aaattatacc tttagtttct ttgcataaaa
     3181 tgatacaatg attgttaaaa ttgtgaaagg gtggtcattt gttagagaag tttttggata
     3241 tgtcgttagt tcaacgtatt gttgttggta ttttagtcgg aatattgttt ggtatctttt
     3301 tccccacatg gacatttatt tcaatattgg gaacgttgtt tgtcagttgc ttgaaggcaa
     3361 tcgcacctat tctggttttt ttcttgacga tggcgtcaat tgccaagcat aaaattggga
     3421 ataagacatt cgtgaagcct attttgatct tatatttagt gggaacattg ctttctgcac
     3481 ttgtagcagt tattgtaagt tcaattttta ctgttcctat tactttacaa gaaacagtat
     3541 ctgaaaaagc accgcaaagt ttggaaagtg ttttatctac tatgttgacg aatgtcacac
     3601 aaaatcctgt tcaatctctt attgatgcga attaccttgg cgtcttattc tgggctgtat
     3661 tgttgggact agcttttcga gcacgttcag aatcgacaaa agagttggtg gatcagatat
     3721 ctatagcgtt gtcacaagtc gtacaagtga ttattgcatt tgctccgata gggattttag
     3781 gattggttta tcaatcaatt gctacgactg gaatagctgg attggcagag tatctgcagc
     3841 ttttacttgt tttgatcggt acgatgctgt ttgtcgcatt agttgtgtac ccccattcct
     3901 cacgtttttg tttattaaag aaaatcccta tccattgatt ttcttttgtt tgaaagaaag
     3961 cgcacttccc gctttcttta cccgaagttc tgccgcaaat atcccgataa acatgatgtt
     4021 ggctgaaaga ttgaaattga caaaagagtc ttattccata tcgatcccgt taggtgctac
     4081 gatcaatatg ggcggtgccg cgattaccat ttccttgatg acgttgactg ctgtacattc
     4141 tttagggatt gaagcacctt ttgttttgaa agtcctttta tgtattttgt ctgctttagc
     4201 tgcttgtgga gcttcaggtg ttgcgggcgg ttcactgtta ttgattccat tagcatgtag
     4261 tttgtttggt atctctaatg atgtcgcgat gcaaatcgtc ggtattggat ttattatcgg
     4321 tgttatccag gattcaatgg aaactgcctt aaattcgtca agtgatctgt tgtttactgc
     4381 aataggagag ctaggcgcgc gtaaaagaag tggggaaaaa atcaaattga aagattgttt
     4441 agatggatta agtgaacgtt gaacgttaga tatttagtat ttttaaaatg attcgagttt
     4501 ttaatgagtt atatagatgt tcgtgtaaat agaggtttct gttaaatcaa gataaagtcg
     4561 cttataataa caattaaaag cataggtaat tgttattgga ggcgtatcat ggatttttta
     4621 cgtaaatttg gttttttctt tttattatta ggcgtgctga gtgatttttt gactccgtac
     4681 gtattaggat tgtattatcc tgaattagat caaatgaaga gcgtgatcag tgtatttgga
     4741 gaggtttcca gtcctgtaag aaaagcgttt cttatttggt ctgtggtttc agggatcttt
     4801 ttcatcctgt cattgcctgc attgtatgct gcatttgtaa gtacatctcg tgtgttagct
     4861 accctagtta cagcagccat cgcgttctat ggtataggtg attgtatctt tacaggtttg
     4921 ttcagtatca atacccatga atcttcgtgg aatgtctcga catgggtcca taatatcgga
     4981 tcaggtttag gttacagtgg ttttttattg tttccagttc ttcttttctt gatctatcga
     5041 aaaaatgggc aaatggacgt aagccactta taccttggac tgtctgtgat cagtttgctt
     5101 tttgcggcga tttatggact ggcaagaatt ccttctataa attcttggcc attactgaat
     5161 aaaataggtt tttgccaaag aatcagtttt ttctttaatt atgtgcccat tgcctggttg
     5221 agtatcttgc atctcaaaca gtaaaacttg agccgtcaaa ttgaacagtc agcggatact
     5281 tcatccggtg gctgtttttt gtttttctga aatatatatt tccacagttt tcttgtatag
     5341 cttctgtttt agagaagaaa ggagctatat tttgtgctac aatcttaaaa aggtagagag
     5401 aagaccaaat ggaagacaga ggagccacta aaatatgaga aacatcaagt tgacaatcga
     5461 atatgacgga aaaagatatt caggatggca aagacttgga gatgacgata aaaccattca
     5521 aggaaaaatc gaaaaagtat tgcatcagat gacaaatgaa gagatagaaa ttattggatc
     5581 aggaagaaca gatgctggca cccatgcgcg aggacaagta gcaaatttta aaacaaacac
     5641 agaaattgga ctggcagaaa tgatcgactt tatgaatcgt taccttccgc gggatattgt
     5701 catcaaaaga atcgaagaaa tgcctgagcg cttccattca agatataatg cggtgggcaa
     5761 aaaatacagc tattatgttt ggaacaacgt gattccttca gcatttgagc gaaaccacag
     5821 cttttatttc cctcaagagt tggatatgga caagctgaat gcagcttgcg aaaaattagt
     5881 gggtacacat gatttcattg gtttttctgc attgaaaaaa tcaaaaaaat ccacagtgcg
     5941 tacgattgat gaattatcta tcgaacgaaa gggagaaatg cttcatttta cttttgtcgg
     6001 agacggattc ttatataaaa tggtgcggat tatcatgggg acattactag aaatcggcac
     6061 aggtgccatg ccgatagaaa caatcgatga aatatttgaa agcaaagtgc ggcaacatgc
     6121 aggagaaacc gtccctgcac aaggtctgtt tttggatgaa gtttattatc gataaaaata
     6181 ctttgataat ggatgaaacg catgtttcac agtataaata tcgatgttaa ataatgtatg
     6241 aagcaaatta aagaaatata aaaaaagact ttttagcctg tttatgtgta cagctaaaaa
     6301 gtctttctta aataaaaatc tagtgagaat gttgtacttc tcttctcggt tgtttttgtt
     6361 tagaaaaagt aaatcgataa agtaaaaata aaatcaaagt aacgatagat aaatatcttg
     6421 aagcaattgc tagttttgtg tactgataag taataacgac tttatttata cctggaggaa
     6481 tagttaattc agttgtgcca aacttagata attttgtttg tactagtttg ccattaatag
     6541 aagccacttg ccctttataa cgataaatag gtgtcgtcaa gacagtattt ttattattcg
     6601 aattatctaa ttcaattgta agtttgttac tcataaaatg agttgttgga tttatttcct
     6661 ggttacctag taaatattga tcattattaa tcatatcagg atgatagatt gactctttgt
     6721 ttgcataatc agtatgattg taattttcag ctatagcaac agcgtcttca ggtgctataa
     6781 gtgttaatga atctttgtta gcatggatag aattgcttac tcctgagaga tgcaatatta
     6841 gtgcaagaaa cgtaaagacc attattttcc atgaattatt ttt
//
LOCUS       DAJSKS010000133         6816 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJSKS010000133 DAJSKS010000000
VERSION     DAJSKS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.137"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..1140
                     /locus_tag="ON396_002365"
     CDS             <1..1140
                     /locus_tag="ON396_002365"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCR3114420.1"
                     /translation="DRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAVNRANE
                     DIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFARSFEV
                     KEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAMMITQN
                     PRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKELSGRG
                     LEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNLYTGSL
                     YLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQEMLDSR
                     TRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1162..1914)
                     /locus_tag="ON396_002366"
     CDS             complement(1162..1914)
                     /locus_tag="ON396_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3114421.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2278..4944)
                     /locus_tag="ON396_002367"
     CDS             complement(2278..4944)
                     /locus_tag="ON396_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR3114422.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5285..6736
                     /gene="cls"
                     /locus_tag="ON396_002368"
     CDS             5285..6736
                     /gene="cls"
                     /locus_tag="ON396_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR3114423.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2007 a         1412 c         1238 g         2159 t
ORIGIN      
        1 gatcgtcttt ccttttttat tcctaattta tatttagata tgacagagtt ggcagaaagc
       61 cgtggggatg atcctgcaaa ataccatata gggattggtc aagaccaaat ggcagtcaat
      121 cgtgcaaatg aagacatcat tacactagga gcaaatgctg ccagcaaaat cgtaacagaa
      181 aaagaccgtg agctaatcga catggtcatc gtcggaacag aatccgggat tgatcattca
      241 aaagcgagtg cggtaattat ccaccattta ctgaaaatcc aatcttttgc tcgttctttt
      301 gaagtaaaag aggcttgcta cggtggcacc gcagctttgc acatggcgaa agaatatgtc
      361 aaaaatcatc cagaacgaaa agtactagtc atagcaagtg atattgctcg ttacggcttg
      421 gcaagcggtg gtgaagtgac gcaaggtgtc ggtgctgttg cgatgatgat cactcaaaac
      481 ccgcgtattt tatcgattga agacgacagc gtatttctga cagaagatat ctatgatttc
      541 tggcgtccag attatagcga atttcctgtt gttgatggtc ctttatctaa ttctacgtat
      601 atcgaatcat tccaaaaagt ttggaatcga cataaagaat tgtcgggtcg aggactcgaa
      661 gattatcagg cgattgcttt ccatattccg tatactaaga tgggaaaaaa ggcattgcaa
      721 agcgtattag accaaacaga cgaagaaaat caggaacgtc ttatggctcg ctatgaagaa
      781 agcatccgtt acagccgacg aatcggtaat ctttacactg gttcattata cctggggcta
      841 acttctctac tggaaaattc gaaatcacta cagccaggag atcgcatcgg tctattcagc
      901 tacggttctg gtgcagtaag tgaatttttt acaggttatc tggaagaaaa ctatcaagaa
      961 tatctcttcg cacaatctca tcaagagatg ctagattctc gaacacggat cactgttgac
     1021 gaatatgaaa cgattttcag tgagacgctt cctgaacacg gagaatgtgc agaatatact
     1081 tcagatgtac ctttttcgat tacaaagatc gaaaatgata ttcgttacta caaaatataa
     1141 taaaatcata aaaagaaccc ttcacgagtc gtgaaaggtt ctttttttat ctgcggacca
     1201 aaggagcagt ctataggtca gcttagtcgt actaaggatt tctcgttttt cactgaatag
     1261 tttgcgaatg tacctggtca caacaggatc ttttgcgtac gcgtcatagt atagcttcat
     1321 caattcttca gtaattgatt cctgtgtaaa ataagtaaaa tcttttttct tcactttgaa
     1381 tccttcatca attaagtatg tttcaaattg atccattaca actggcgatg tattaggatc
     1441 ttcttctatc attcctagtt gcttttcgat aggataaaaa acatcatcga ttgattccac
     1501 tactctgatt atcacgcacc atttgcttga ctttttcatc aatttttcta attgaataag
     1561 atgtaccaat gctggatttg ctgctgtaaa aaccatttca tatgtctcat ttgattggat
     1621 aaattcttct aacgactgtt tgataaagga aacatttggc aactcatttt tttctgcttc
     1681 tttttttgca ttttctagca ttcctgaaga aaaatcgatc aaatcatagc gattaatttc
     1741 ttttgctaag gaaagaaatc tgccagatcc tccagcaacg tctgctgctg tttcagcagg
     1801 caggactttc tccgttttca gataattttt tacatcttca gcaatagtta cttgcgattc
     1861 ttgctggatt tcataatatt cttcagcaaa ttcatcccat tctttttcat tcatttgatt
     1921 tcctctaatt ttttgttata tgttttcact tttcggaata aatcattctc ctcttttatc
     1981 tattctatct cttttgatga ataataaaat gattggcttt ccgtctatca taaataaaaa
     2041 aagggactgt gaccaaagtc gtgtcaaagt ccctctttcc gaataaacag tgtccaatca
     2101 gatgaccctc agtattagct caaaatgctg attttacaac ttcttcctta aacggtgttc
     2161 tcctgccaac tcttcggtca caagtcacaa tattcaacaa acatgagaag ctgtttgttg
     2221 aaattgcacc ttgtgactca gaattaaacg gcaggctcac aacctcgttg attcgtttta
     2281 tttatttcct tgtactcgta caatgatcaa tttagtgatt tccatcaaaa caattgtcac
     2341 aagtgcgatt cccatacatg taaagacttc tgtcaatcca aatgctgatg ggatagagaa
     2401 gacttctttc agtcctggga tcattgttaa gcagtaaagc gcgctacata caactacagc
     2461 tagtaagaca cttctgttag agaagaaccc tgcttggata gctgtttgtg tgtttgaacg
     2521 tgctgggaat gtttgcaatg tccgacacca aatcaatgtt gaaaatgaca tggctacacc
     2581 gaattcaggt gaatgctgca tgccaagcca ttgagcaaag ataacagcaa tcccaatcag
     2641 cacaccacga tacgttacag aaatcaatgt tttgtccgta aagattcctg aatttggatc
     2701 tcttggcggg cggttcatcg tagcaggttc agctttttct gtacctaatg caattgctgg
     2761 cagtgaatca ttgactaaat tgatgaacaa cagttgtaat gctgtaaatg gattggacca
     2821 gtcagcaact aaggcaaaga tgatcgcaat gatcgcacct aaatttcctg caaatagata
     2881 agaaatagct ttcttgatat tatcaaacac attacgtcct acttctactg cgctgacaat
     2941 agaagcaaag ttatcatccg tcaataccat cgctgctgca tcttttgcca catctgtacc
     3001 cgttcccata gcaattccga tgtctgcttg tttcaatgct ggtgcgtcat ttacaccatc
     3061 acctgtcata gctgagatat gacctttatt ttgccaagca cgaacgatac gaattttatt
     3121 ctcaggtgaa acacgtgcat aaacagtgac tttttctaaa acttcgttta gttgatcttc
     3181 tgtcaattca tctagttccg tcccggtcaa agccaggttt ccttcttcaa aaatcccgat
     3241 ttgttttgcg atagcaacag ctgttgtttt gtggtcacca gtaatcatga ttgttttgat
     3301 tcctgcactt cttgcttctg ttaccgcctg agtcacttca gcacgtggag gatcgatcat
     3361 agcaagcaaa ccaacaagga tcaaatcgtc ttcatcttca aatgataaat caaaattatc
     3421 aagtggacgg taagcaaaag ctagtacacg taatgcgcgt gttgaaaaag cttcattttg
     3481 ctcttggaat tttttcatca attcatccgt catcggaacg acttcaccat ttagcaatac
     3541 ttttttactg cgttggaaga cgatatctgg tccgccttta acaaacaagt aatttttgtt
     3601 atcaattgtg tgtccagtag acattaattt tcgatctgag tcaaaaggaa gttctgcttg
     3661 tcttgggtag ttgtttcgga tttcctgata aggctggttt aatttttcac taaagtcgat
     3721 aaacgctact tccgtagggt cgcccaattt agtaccgtct tcactgatcg atgcatcatt
     3781 cgctaaaaca ctgatttcga tcaagcgacg ttcgtcagct gtccattttg aagggtcatt
     3841 tgtgaaatcc ccagtatcgc cattacctaa aaagtagtca acaactgtca ttttgttttg
     3901 tgtcaatgta ccagtcttat ctgtacagat gatacttgtc gaaccaagtg tttcaactgc
     3961 tggaagcttc cgaataatgg catgacgttt tgccatttta ttcgttccca aagataacac
     4021 gattgttacg atagactgta atgcttctgg aatagcagct acggctacgg ctacggcaaa
     4081 catcactgcg ctgacgatat ctgcagtcat atctcctgta ccttctccaa gataaatacg
     4141 aaacagttgg atacctaaaa tcaataaaga caatgccaaa atagccaaac ttagtttttt
     4201 actgaattta tccagacctc gttgtaacgg agtgacttgc tcagttgttg attccaaaag
     4261 gccagcaact tgtccgatct ccgtattatt cccagttgaa gtcactaaga atttaccgcg
     4321 tccgtaagtt acgatcgttc cactgaatac catatttgta cgatctccga ttggtaccgt
     4381 acttgtgata tccgttactt ctttttctgc agaagtcgac tctcctgtca gcataccttc
     4441 atctactttt aatgatcccg catctagtaa acgtccatca gcaggtacat aatcgcctgc
     4501 gtctaacaac acgatatctc ctaccacaag ttcgtttgcc ggaatcgttt tttccttacc
     4561 atcacgaatc actcgagcat cgggcgctga aaggtttttc aacgcttcta aagaaccttc
     4621 tgcttttttt gtttggacaa cactgattat agaattcaac atcaatactg caaaaataac
     4681 taagaattcg atgtagtcac ccatgaccat ctgaatcagt gcgacaatca gcaatacgat
     4741 aaccattgca tctttgaaag tttctaagaa caatttccaa gttggcttct tcggcttttc
     4801 ttttagctgg ttcaagccat ctctttctaa tttttccttc gcttgcgtgg tgctcaatcc
     4861 atcggttgag ctctgcgttt gctgcatcaa cgagtcgatc gtttctttat agaccgcttg
     4921 ttgtgcttgt tccttgtttt ccaaatttca tcccccattt tttgatcttg gccctagtcc
     4981 tgtctgaaaa aaaagagacc taagacaaat acgcatttgt cttaggtctc accatttcat
     5041 acaataccag aaaaccattt ggttaactta ctgttgatat tatcgcaaca aacgttgccg
     5101 gttactcccc taaggagata ttcaactgta ctaatatcct accttattaa cctacaaaaa
     5161 gcaactactt tagcccgatt tacctacatt ttcatgaaag gagtgaaaag tatattcacc
     5221 ttggtaggct tctttcttct atcatgctat actaatgtta ttgtatgtaa ggaggaacat
     5281 ttttgtggta tctagtatta taaccgccct ttatctatta aatgcactta ttgctttggt
     5341 ggctattttg atcaaacccc gagatgtagc agccatttgg gcatggctct tagtattgtt
     5401 tgcccttcct ggggtgggct ttgtattata tttatttttc ggacgtggat taacggataa
     5461 gaaaaaattc tatctccgac aaagtgactt gaaagaatta gaaaactttc agtcttttag
     5521 aggagatacc attgaacatt acgatcctga catgggcgat aaagacaaac agcaatttgt
     5581 tgacttcttc tcttcattaa atcgtatgcc gctgacaaga atgaattctg tcactcttct
     5641 cacagacgga caagagaaat tggattcact gcttcaagat ctaaaaaaag ccaaacattc
     5701 gatccatatc gaatattacg catttgtgac agataatatc ggccagcaag tcttacatgt
     5761 tttagaagaa aaagccgcag aaggcgtgga agttcgaata ttatatgatg catttggctc
     5821 tcatggcaca aaagcaaaag atttcaatcg tctaatcaaa aatggtggac atgtccatac
     5881 atttgttacc tcacaaaggg cattacttcg tttccgattg aattaccatg atcaccgaaa
     5941 aatcgttgtg atcgatggaa agattagtta taccggtggt ttcaatattg ccaatcaata
     6001 tgtaaataca acaaaaaaat tcggctattg gcgcgatacg catatacgga ttttcggtgc
     6061 cgcttcttct ttgctccagc ttcgcttctt aacagactgg aacgtctcgg tacctgaaga
     6121 aaaaaaggtc ggctatcatt tgaattattt ctttaaaaaa gcagatcgag atgaatctaa
     6181 gcttgctgat acatccatcc agcttgtttc aagcggaccg aataacgaaa gggaacaaat
     6241 caagctttca tttatcaaat tgattacttc tgctaaaaaa cgtgtttgga tacagacacc
     6301 ttaccttgtt cctgatgaaa gtgtcattgc tgctttaaaa atcgcaactg cctctggtgt
     6361 agatgtgaaa atcatgattc ccaacaaacc ggatcatcct tttatttatc gagcaacaca
     6421 atattatgct cggcagctga tcaaggaaaa tgtacaaatc cttgtctatg agaacggctt
     6481 cctccatgca aaaacattga taatggatga tgaaatctgc atggtaggtt cagcaaatca
     6541 agatattcgt agctaccgat tgaattttga aacaagtgct gtcatttacg atcctgagtt
     6601 tttagaagaa cttgctactc agttcaaaga agatgagaca cattgttcat ccatgacaac
     6661 tgaaacagtc aaggaaatgt ctaactggct attattcaag caacaaattt ctcgattatt
     6721 ttctccaatc ctataatttt tttattcaag tagagaaaaa tatactaaga ttaaagggac
     6781 tgtgacaaaa atcttgtcac agtcccttct tccgaa
//
LOCUS       DAJSKS010000134         6756 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJSKS010000134 DAJSKS010000000
VERSION     DAJSKS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.138"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            49..939
                     /locus_tag="ON396_002369"
     CDS             49..939
                     /locus_tag="ON396_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR3114424.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(1012..1836)
                     /gene="nadE"
                     /locus_tag="ON396_002370"
     CDS             complement(1012..1836)
                     /gene="nadE"
                     /locus_tag="ON396_002370"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR3114425.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1848..3320)
                     /locus_tag="ON396_002371"
     CDS             complement(1848..3320)
                     /locus_tag="ON396_002371"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR3114426.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3957..4319)
                     /locus_tag="ON396_002372"
     CDS             complement(3957..4319)
                     /locus_tag="ON396_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCR3114427.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4542..5294
                     /locus_tag="ON396_002373"
     CDS             4542..5294
                     /locus_tag="ON396_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3114428.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5434..6357)
                     /locus_tag="ON396_002374"
     CDS             complement(5434..6357)
                     /locus_tag="ON396_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3114429.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6372..6644)
                     /locus_tag="ON396_002375"
     CDS             complement(6372..6644)
                     /locus_tag="ON396_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114430.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2066 a         1350 c         1156 g         2184 t
ORIGIN      
        1 taacaaatat gttaatatat aagaaaaacc ttttaagagg tgatatatat gcaactacaa
       61 ataaacgaag aatccttacc ggtatatgaa gcattagcta gtaaaacaag gatcaagatc
      121 atccagctac tttccaaaaa aaagatgaat gtcaaagatt tggcaaaaga acttggagtg
      181 agcagtgcta tcacgacgat gcacgtgaaa aagctagagg aagcaaacat catcaaaaca
      241 gaaaaagtag gacaacaaaa aatttcgagc cttcgtgtcg ataaaatcga tattagtttt
      301 ccagaaaaaa tattcaatgc ttttgatacg aaagaaacat cgatacctat tggtcactat
      361 acaaattatg caattgaacc aacatgcggt ttagctacga tccatgattt tatcgggaaa
      421 gtggatgaac cacgctattt tatggatcct cgaaggatgg atgcccgcat tctctggttt
      481 accagcggat ttgtcgagta tcaggcacct aattttttaa atacagagga tacattggag
      541 atgttggaag tttctgtgga aatttcttct gaatttcctt tttcaaatga caattggcct
      601 tcagatatca ctttcagcct aaatggtgtg gagctaggaa catggacgag ccctggagat
      661 tttgctgata tccgcggtaa atatacgcct gattggtatc cagataatct taatcaatac
      721 ggcttgctga agacgatccg aattaccaaa cacttgacga atatgaatgg agaacccttg
      781 tcaaacatca cgatcaacga catccctaaa gagcaagaca cttggcattt gcgaatcgaa
      841 gtgaaagacg atgccaaaca tgtggggggc tgtactttat ttggtaaagg attcggtaat
      901 tacgatcagg atatcaaatt aaaagtttac tacagctgat atttttcttt tctattttat
      961 aaaacaaaaa cagccaatcc ctatgaaggg acggctgttt ttgctttatt gttatttcca
     1021 gaaatcatcc aaaacggaaa ttggcaaatg tcgtttatgt tgtgtcttgt tccaccatgc
     1081 ttcgattttt tcttgtgctt gagcgggaac ttcttttcct tctagataat catcaatttc
     1141 attatatgtg acacctagtg cgacttcatc tgcaatcatt ggtttcccat cttcaagatc
     1201 tgcagtcgga atttttgtgt ataatttttc cggtgcattc aatgcttgaa gcaattgttt
     1261 cccttgtcgt ttgtccaaac ggaataatgg caaaatgtcg gctccaccat caccaaattt
     1321 cgtgaagaat ccagtaatgt tttctgcagc atgatctgtc cctaatacgg ctcctgctct
     1381 ttctccagct acggcatatt gcgtaatcat acgttgacgt gctttgatat ttcctttgtt
     1441 aaaatcgctg attcccacac ctgcttcgct taatacttgt acttgtgcat caacagctgg
     1501 tttgatattt actcgcagac taacatcggg ctggataaag tcaagagcag cttttgcatc
     1561 ttcttcatct gcttgttcac cataaggaag acgaacagcg ataaattgat agttttcatc
     1621 ttttgtttct tcacgcattt cttccatcgt tagttgcgcc aaacggcctg ctaaagtaga
     1681 atcttggccg ccgctgatac ctagtacaaa agttttaaga aacggatatt ttttcaaata
     1741 tgctttcata aagtcaatac ttttacgtat ttcttctttt ggatcaattg ttggtttgac
     1801 acctaattca ttgataattt ctgtctgcag gctcatttat tctcctccta tgcttctgta
     1861 tgaagttctg ctacgctttt tttcattcgt tccatgatag ccattttatg gttccagcat
     1921 tcagtagaca gatcgactgg gtatttttgc ggattcaagt ctcgtttata ctcttcccat
     1981 agtgaatcta gattttcttt tgtatattct ttgatttcgt ttagtgcagg taattcgtaa
     2041 actctttttc cttcgataaa gatatcttgt aaaactgggc gagctgtaaa gtctcgaaca
     2101 gttttattga taaacgtatg cactggatgg aacatgaaga tagcttcttc ttctcgaggg
     2161 tcttcatccc ataatgtcac gtagtctcct tcagatttcc catcaaaatt tcgagtgatg
     2221 cgccacactt gttttttccc tggagtagtt actttttctg cattgcttga taacttgatc
     2281 gtatccatca tttttccttc atcatcttcg atcgatacta gcttgaatac tgcaccaagg
     2341 gctggctgat cataggctgt gatcaatttt gtcccaacac cccatacatc aattttagct
     2401 ttttgcatct ttaaattcaa aatcgttgct tcatccaagt cattcgaagc atagattttt
     2461 gcttccgtaa atccggctgc atctaattgt tcccgaacac gcttagatat ataggccata
     2521 tctccactgt cgatgcgtac gccttggaag ttgattttgt cacctaattc tttcgccacg
     2581 cgaatcgcac ttggcacacc tgatttcaac gtatcataag tgtcgactaa gaacacgcag
     2641 tctttatggg ttttagcgta ggccataaat gcttcataat catttccata agattggact
     2701 aacgaatgag catgggtccc gctagctggt atcccaaata tcttcccagc acgtacattg
     2761 cttgttgcat ctgctccgcc gatatatgcc gctctagttc cccagactgc tgcatccagt
     2821 tcttgtgccc gtctagttcc aaattccaat agtggatctt ctccgatgac agatttgata
     2881 cgagctgctt ttgtcgcgat caacgtttgg aaattgacca tattcaacag tgctgtttct
     2941 actaattggc agtgcgctaa aggcccttcc acttggatca atggttcatt tgcaaaaact
     3001 agttctcctt cacgagcaga acgaactgtt gctttaaatt caaaattttg taaatactcc
     3061 aaaaaacctt caggatagac ttctaatcca cgcaagtaag caatatcgga atcattgaac
     3121 gtcagattct ctaaataatt gactaaccgt tccaatcctg caaaaaccgc gtatccgtgg
     3181 ttaaaaggca tttctcgaaa gtaacattca aaaacagcat gtaaatcagc tcgacctaat
     3241 tcccagtatg tctgcatcat attgatttga tatagatccg tatgcagcgt caaactgtcg
     3301 tctgggtaag tattattcat cttgtattac tcctcttggc aagttttcat ttcctaccca
     3361 ttataacaaa aaaaattaat aggaaaagaa atcttccatt ttttctgaaa aagcacatgt
     3421 tttacggata tttcttgcta aaaatgaact ccatctgcta aaactatcct ttcttattgg
     3481 tatttaacct aaacgagaaa gaatacttat tttattcaat gtatccgaga cacgaagaaa
     3541 ttagcgtttt ctatttttct tacttgataa aataagtaga ttttcctact acaatcgtag
     3601 ccgttaagat accgatgatt gaacaaatat aaccaaatgt ctctacaaca taatcaatgg
     3661 caaaagtttt tgaagcaata atacgtcttt ccttttttgc ttcttatccc caaagaaacg
     3721 gatataactt attatgtatt gaattttttt ggaaaggcga attatgacaa gatatacctt
     3781 gcgttgaaaa aactcgcgga aaaaatatca cacgttttta aaaagataca aagtaagaaa
     3841 gcagctgtca atagaatgga aacaaaaatg gcgagacatc aatcagtctc gccttgtttt
     3901 ttattgtata cattagatat ggagcaggtt accataaaga tgattattag tcgttattaa
     3961 atagaatcct tcgtattttt ttgttctatc actacaatat ctttatttgg acgtttgaat
     4021 tgtattccgg cacgttctag ctcctgtttc actcctacat ggactttgac atcttctcgt
     4081 gtcattttta aaacgtgatg aatcgcatag tcgatttctt gccttgtgat ttttttcttc
     4141 gtattgatca tcacgacttg ctttttatca tatgtgtcta ccaatagcag ttgtgttcga
     4201 tctaacgaaa ataatttgta ataattaata gccgtattcc caaaaaagaa ttccactact
     4261 ttaggataaa atgtcttaat atttaaatta ctattcacag gtgtttcgat tagcttcatc
     4321 tcattcctct tttctatata gtcgatttag atccaatctt aagttatgtt accatctttc
     4381 ctagcattaa ggaatcggtt acaattgaac ctttaaagac aaaaggattt accttttatc
     4441 acttaatagt ataacataat ttcatgagaa aaaaatacaa ttttttcttg accatctcgt
     4501 tacgtaaagg attatgctta catttgtcag gaggaaaaat aatggagtac acaataaaga
     4561 aaatgtcaga gatatcagga gtcagtccga gaaccttgcg cttttatgat gagattggac
     4621 tgctaaaacc agctaggatc aattcttcag gttatcggat ttatggcaaa aaagaagtcg
     4681 atcgtctgca acacatctta ttttatcgaa caatggcatt caagttggat gatatccaag
     4741 aggtcttaga caatccatct tttgaccacc agaaagcttt aatcaagcat cagcaaatgt
     4801 tgctggaaaa acgggcacaa atcgataccc tcttgacaac tgtccaacag acacttgata
     4861 tgtacgaagg aggaagaaaa atgagtgaca aagaaaaatt tgaagggttt aaacaacaaa
     4921 agttgaaaga aaatgaagaa tcctatggac aagagattcg acaaaaatac ggaaaaaaaa
     4981 aggttacagc atccaatgaa aaatggatga atatggataa agaaacgtat gaagaaatgc
     5041 aaactgttga aaagcagatg ttatttgatc tcactcttta cttaaaagaa ccagaagatg
     5101 aaagcttagc agaccgtatc ttccaagctc ataagaagtg gttatcttac agctggcctg
     5161 aatatcagcc aaaagcgcat aaaggactag gaatgatgta cgaagccgat gaacggttca
     5221 ctgcatatta tgacgagcga agtggtcatg gtgcagcaaa agctttaaat gaaattatcc
     5281 aaaaaaaggc gtaatctttt acatgatcca acaaaaaagt acacgtaaaa tgacctaata
     5341 acaaaacagc agacacccaa gttagatttt tatgctaact tttggtgtct gctccattct
     5401 ttcacaagtt ttttcatcct actttaaata tattcatcgt tgaataaacg tataaggtat
     5461 tttcacttta tccttttttt ctgtaagaaa caattgaaat gccatttctc cgcaagcttc
     5521 cagatggtgg tcgatggttg ggaaaccaag aagctcgctg atcagcaaat tatcgcgtcc
     5581 aatcaccact ggtgtcttat cttgcagata gctttgcaag atgcctgctg caaccatatc
     5641 cgaatttgta aagacaccat caactccaag ctctttaaaa aagaagcctg cttgttttcc
     5701 tttctctgca tctatacaat cccaaaaaac aagtgattca tcaaaagatg gaaaaagttg
     5761 atgacatagc tgcaaagtga tttttgagtt ataacttagc ctgccacttc gccccaacgt
     5821 gatgcccaat tgctttatac cttgctgctt caaataagta agaccttctg taattgattg
     5881 ttctcgatca ataaagacac agcttgcctg ttttcccttg atttcttcac aaaaaactag
     5941 aggtccatat ttttgatatg ccaataaatc atctagtgta ttggcacgag acgtaatgat
     6001 caatccatcg aaagctttag ctgcaaattc ttcaagatac cttcgctctt tttctttctg
     6061 atagcccgtc ggtaaaagtg tcactttgta atcataatca aacgctgctt caatgatccc
     6121 actgatcaac tgatcaaaat acggatgatt ggtataaggc agaatcactc caatgctttt
     6181 cgtttttcca taacttaact gtctggcatt tgcattaggg atataatcga cttctttcat
     6241 gaccgcaagt atttctttcc ttttttcctc atctacatat ggatgtccat tgattacacg
     6301 tgaaatagta gaaactgaat aacctgtgag ctttgcgata tctcgaatgt ttgccactgc
     6361 tgcttcgcca cctatacaaa aagattttct tgtaaatagt ctaccacagc tggaccgcat
     6421 tcaaaaaact gatacatttt tccttcatgc gaaaaactga tccgctgccc gccccaatat
     6481 atatcttttg tcaaattttc gatttcattc attggtattt cttttactat attcctattc
     6541 aacccttgaa aactgtacaa cgttaatacg gatcccttga actcgacaaa cgcataaaaa
     6601 taattgttcg ttgtgccatc tgttatctct acataagtat tcattgcaag ccctcctttt
     6661 gatactttga gtatagtgta tgaaacgcgt ctcatagcaa ggagtttgtt ttgccaaaga
     6721 aaagttttat tcctctttca ttattaccaa agtctc
//
LOCUS       DAJSKS010000135         6568 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJSKS010000135 DAJSKS010000000
VERSION     DAJSKS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.139"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..1529)
                     /gene="pglX"
                     /locus_tag="ON396_002376"
     CDS             complement(<1..1529)
                     /gene="pglX"
                     /locus_tag="ON396_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HCR3114431.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AV"
     gene            complement(1593..5264)
                     /gene="brxC"
                     /locus_tag="ON396_002377"
     CDS             complement(1593..5264)
                     /gene="brxC"
                     /locus_tag="ON396_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HCR3114432.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(5289..5864)
                     /locus_tag="ON396_002378"
     CDS             complement(5289..5864)
                     /locus_tag="ON396_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HCR3114433.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(5864..6463)
                     /locus_tag="ON396_002379"
     CDS             complement(5864..6463)
                     /locus_tag="ON396_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR3114434.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         1931 a         1248 c          873 g         2516 t
ORIGIN      
        1 accgctacat atttatcaac tagcatttca gccacgtcaa gaatgtgcaa gtatttttct
       61 tgtatttcct tcatcccata aagaatatcc atattcgtaa attcgttgaa ttttgatata
      121 tactctctta cttttcctaa gtattcttta gttattgcct gattattttt atcgactaac
      181 ttaatgattg atccaagttc tttcccattt tcaaattgat caaaaattaa ctctttgtat
      241 tcattccagc gttcactata cttatcattg ttcaattcac taatacgctc ttgatactcg
      301 tctgtcactt taggagaatc aataaactct cttaaattcg gcgatagact ttttttcaaa
      361 aaaagacgat cgtattgacg ccctttcatc ataattgcaa aataagacaa ttgatacgct
      421 cttttatcga tttcaagacc atataaattg tattcaacga ttgcacgtgc agcatcacgc
      481 tcagaatatc ctaacgtaag ataaatttcc atcaatacat cgaaggcgta aactaaaata
      541 tgcccagacc ccattgcgtt atcgataatt tttagttctt ctggttttaa attacttaca
      601 gatgttgctt gttcaaaagc atatttatct ggaattaagt atttcaactt atttcttaat
      661 tcactattgg gataatgatc taaccaatac tttcctaaac tattgtcaac catataacga
      721 acgacccaat ctgtggtaaa tagttgagtt gctgccggaa tatcttgttt tttgacaatc
      781 cctttataaa tattcaccac ttgattatgt ggctcactat tatagtactg atacaaccac
      841 ccaataatct ctacctgtcc accagcttga acatagaaat catcctctgg aatacttaaa
      901 agatcagcga ttacaccatc ttttttaata taagaagctg taaacaaaag ttctgtataa
      961 tcttctactt cttcaaatag ctcaggtaag taatcattca attgattaca gatttttata
     1021 aaaatcaatt gatacagctt gtccattgct tctgctgaat tatctgtttt ccaagttctg
     1081 acttgttgct gttcattact agataattct tcaaagatat cggcatcata aatatctgta
     1141 ataatatctg gctcaatttt cccacttatt tcactagata atacacgata cgtgtcaggt
     1201 aaataatcat ttacctccat aaaccgaatg gcaatcaatc ggttaaacca cgtataagca
     1261 acctcttcga ttaactcttg aaaagcttct gaataagaag tgacagaagc acgttgtttt
     1321 agttctgcaa tcaatttgtt tctttgcgct atttcttttc cttgaattct tattcctgat
     1381 ttttcgattt cttgtaaatt gctatcaatt ttcttcacat cttcaatttt tgattcatca
     1441 attcctaaac gttttgcttt agtcattact tcatcaatta attttctacg ggcattgacc
     1501 gcgaaattct ttatcgcttt cttatccatt tttctttccc ttctatatgt aacctttgaa
     1561 aagacaggca cctttttatg cctgtctttt cactagaatt gaatcgtgat ttctgtattt
     1621 tcttgaagtt gttccttcag tttttgatta atttcagctg ttaactcatc aatatctgct
     1681 gctgacttaa tagttttaac atgaacgtta agtgttctca ttggtactgt tttctgttga
     1741 atagtaggtt ttactatcgc tatttcttca gcttttgctg cagagtctat ttcatctttt
     1801 tcagtttcac gaatcaagtt tgctgcttca atttgtttac gtgcttgttc ttcttgacgt
     1861 ctgcggtttt cttcattatc gaaacgttga ttaaagatca aatactgttc tgctacgttt
     1921 aatgataaga aaacaagatc atctatagta ttcgcttgtt caatttttgc tttcagttct
     1981 tgaaattctt ggtcaactat tggtacaaat tgatccttaa aatcttttcc ttctaataat
     2041 ttaaatgttg cttgtttttc ttccgttaat ttatttaata tttcctgact ttccaactct
     2101 aacagttgat tatattgttc aataaattct tgaatcaact catttagttt catcatatct
     2161 gaataaggat cagccttttt caaaatggtt ttcatctcat gaatcaattg atcaatttct
     2221 tggctagaag tttgacgccg acttttttcg attaatctaa ttttatcttg tgcattctcc
     2281 cagatttttt tctgagtact attttgcttg tggaaatttt taattttatc atatgtttca
     2341 gcccagtcga ataattcatc gcgatatttt ttgattgtat aaaaaaagtc ttcaacatct
     2401 gacgtttgaa caattacttc aagatatttt tttatttgct catattcata agcactaggg
     2461 taggtataag tgttttgtaa atcaaaataa gagcgtaaat tgtctaattg accttttaaa
     2521 tatttcttga aattttccat tgtatgttca ttgttatttt catcaacaat attacgactt
     2581 tgaaacagtt ctctaccgat ttcgtcaacc aatttttttg cttttggcga aatcaccgcg
     2641 cttggttcaa tttttatctt ttcactttct cgtcgttttt gaataatcat tacaatacta
     2701 tttggatcat ccatccgtga aatttgtaca ttgttataaa gtaaatcaat tttaccagac
     2761 atgaaaagtt tagcgatgca ccattccaca tcaagttctg tgaatccata cggttgtttt
     2821 gtttcaaaac gatcacgaat tgttttcatc gtaatcgtta ggtgatttct cttttgatca
     2881 ttgataaaat tgaaaatttc ctgaactgct tgctcatttg atggatcttc aataatttca
     2941 tttttgcttt ctttcactaa atataagata tcttcttttt ctttaggcgc atcaatttgg
     3001 tttaatttat ggtatacttc ttcagccatt ttctgaagtg ctgattggaa ttttgcttta
     3061 aagtcggagc ctgtcagttg aatttctgca ccgttataga aaaattttgc agatttcaac
     3121 agttcattta attgatcgtt gatacttgca cttagttcct gtaattcacc agatttttcc
     3181 gaacgaatcg cgtcaaagtt ctttactaca ctcgaataag tcgttgttaa ataaagattc
     3241 attctagcat acattgctat atcatcttta aattctttat ctgatggcaa ttctacgaat
     3301 aataagttac gggaactctt aagcgcaagt tctgcctctt gacctgctaa atcagagtat
     3361 ggtgaaataa aattaactgt catttcagca tttccagctt gcttgtatgg acgttgatct
     3421 aacacttgtg cataagtaaa cgtataccgg tttttaaatt tatttccacc tgacatgcct
     3481 aatttttcat aggcaaaact atttcggtct agaacgccgt caaaaattcg tttagatagt
     3541 tctacgatca aatcactatc tgtaatattt tgatttttaa tctcacgagt aatttcctgt
     3601 tcttcatcag tttggaaaat ataactatct aagttcttcc gcacaaagtt ttccttaatt
     3661 aaaaggtcca atgcttcttc tactcgtttt ttagtttcca ggcggtcttc gttgatattt
     3721 cttatcatca agctcgtaat attttcaaca tttggtttga tggcttgatc aacatattta
     3781 atcatgaata aaattcgtaa gacatttacg ttaaagttat ctatatcagt tggtctttga
     3841 atatgagaat tatttgtagc ttgtgtaatc actcggctaa ttgctgtatc aatccaacgt
     3901 tctaacgagt aataaaactg atctaaagaa accaaactgg ttgtatcatt ttccttattt
     3961 cgttcagcgg cttctttaaa cattgctagt aatgaacgtt caccagacga taagtttgat
     4021 ccttgatagc tgtgattacg aatctcggtt agaatttgcc ccaataaatt gaattgatag
     4081 ggaataaatg gataaacttc tgcgtaattt tcagcatctt ggaagagctt taattcagga
     4141 ctatcaacaa agagtaattt atttttcaat tctgtttgaa cagtttcaaa ttgtaaggct
     4201 aaagaatcat tggcaaactc ttttttcttc aaaatacgtt ctttaattac ttcatcggca
     4261 tttgctgaag ataaactcac agttgtggca aaacgatcct tgatcttaga aaaatcatta
     4321 tcagaattgt tatcaaagtt tgttacgtga tcaatcgctt cctgacttgt cactaatacc
     4381 caagccttac cttcacaaat tcgacccaaa tcttccacaa cggtttgtaa ctgcaaagtc
     4441 aactcacgat taccgccgat atattgtccg acctcatcga ctagaaaggc aatatgatgg
     4501 tctgcttctt tggaatcaag atagctttta actagatttg caaagtcatc aatgcttatt
     4561 aaataatttc ctgatgcttt ttctaaaaaa ttatcaatag cggcttcact cataaaatcg
     4621 attttaagtg ccgcttcttt gatatcatct tggatccaat caaactcatg gcgctcttct
     4681 tgccattttg ccccattaat ctcttcaaaa gcagctttga atgcatcaaa cttttcttct
     4741 ttaattaacc gacgttcaaa atctgctaga tgtggaatcg tgcttaatcc ttgcatttcg
     4801 ttaaatacct ttaaaaaaac attcacgatt ccgtttttgt cttttttacc acctgcatca
     4861 ctttttgaat caatattaaa cagaataaca tctgtattta tttgtgagca ccgttccatc
     4921 gttgccaaag tcagtggatc tttgattttc ccatcttctt tgaagtaatc aatggctttc
     4981 ttgtcattta tttctcgatt tgcgagcaag taagaaataa tttttaagaa atgagattta
     5041 cctgaaccaa agaacccctg aatccaaaca cccattttat ctgttggtgt attgatactc
     5101 cgttggtagg catcaaaaat tgttttaaag tgtttttgta gttctttcgt aaccacgtat
     5161 tcttctaatt cttgttgaac attttcatcc tttgcttgac cgaccttaat aactccttga
     5221 atatttcggt caattggttt tgcaaaaata tctttaatta gcatttaatc tcctaccttc
     5281 catcccaact aaattctaaa tgctcgataa taattatcat ctttattggt attaagaatt
     5341 gtcaaactta attcatcata ttcccctgga tacatcatca caactggtac gcgatcaaat
     5401 acctgatgca ttttatttaa aatagagtgc gctctaatta atggatataa ttcgccaatt
     5461 cctgtcagga acacaactga tttttctggt gtatgggcat ccacatattc aaaaaataca
     5521 ttttcttcct caggtaaatc cattttcaat aaacgaccaa tgctttccac taatttttgt
     5581 aaaccacgtc gttcttccat cttaatcgct ttttcagtga aaccgcgttt ttctagatac
     5641 tctaacataa tcacatagag attataaact ttcacttcat aattatcacc aacagatgtt
     5701 ccagatatat cattaatata atctcttaca attaactcgt ctttcggatc gtagtcaaag
     5761 atataatagc ctacttcatt acctaaacca cgattcaata aaaaatcttc tttttgaatt
     5821 acttcattca acttttccaa atgctcttga atcgataatg acattataaa attcctccca
     5881 gtgctagtac aatatcttta cgattcttgt caactaacca ttgaatcaat cgtttatcta
     5941 ataatatcgg agtcattcgt ccatattctt tttcccctct gactaatcct gtttcaatta
     6001 gattcgtttg ataagcggca ataagccgtt taatcgtata atctttccat tttgaaatat
     6061 cttctgaact tctccgcttc tctgtaaaaa aagaacggta atcaactggt gttaattgca
     6121 aatttccttt acgtagattt tcagcatata cctcagtcat aaattcatta aaaagatcat
     6181 caatcataag gatggaaatc aaattcacta acttttgatt atctatattt aactgattga
     6241 aaagttgcaa tccagcttca tccagttgac taactctcct tttcacgttc tgatagattt
     6301 ctttttgacg gttactgctc actgcaaaaa aaatattttc atgttcattt ttctctttaa
     6361 tttctatatc tgtatatcct tctagtcgta attgaatata taacttgaac tctgaaaacc
     6421 aaaagcgttt agaaactaga ccagcagaat attttttatt caatagattg cctcctaatc
     6481 atctataatt caataagtaa acaagtgttt tttgctcatc gttagcattc attttttagt
     6541 atctactata caatacatat ttattata
//
LOCUS       DAJSKS010000136         6413 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJSKS010000136 DAJSKS010000000
VERSION     DAJSKS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.140"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..677
                     /locus_tag="ON396_002380"
     CDS             <1..677
                     /locus_tag="ON396_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HCR3114435.1"
                     /translation="LSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQGMLEL
                     LGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFRMI
                     FAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFFGQ
                     VLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAKKY
                     YPGKGQ"
     gene            679..1623
                     /locus_tag="ON396_002381"
     CDS             679..1623
                     /locus_tag="ON396_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HCR3114436.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1822..2466
                     /gene="pcp"
                     /locus_tag="ON396_002382"
     CDS             1822..2466
                     /gene="pcp"
                     /locus_tag="ON396_002382"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCR3114437.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2545..3822
                     /locus_tag="ON396_002383"
     CDS             2545..3822
                     /locus_tag="ON396_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114438.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            4080..5297
                     /locus_tag="ON396_002384"
     CDS             4080..5297
                     /locus_tag="ON396_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR3114439.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(5618..6367)
                     /locus_tag="ON396_002385"
     CDS             complement(5618..6367)
                     /locus_tag="ON396_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HCR3114440.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2059 a         1203 c         1345 g         1806 t
ORIGIN      
        1 atcttagcct tttaggcgtc ttgattgtta ttctcgggtt tgcatttaaa ttagactcga
       61 tattgatcgt aatggttgct ttagttgtta cagctttgac tggcggtctt ggcattcaag
      121 gaatgttaga acttttaggt acaagttttg taaacaatcg cgggatggcg atttttatca
      181 tcattatgtt ggcaacagga acgcttgaaa gaaatggtct gaaagaatca gcggcttcct
      241 tgatcaagcg attcaagaaa gtatctgctg gtatgattgt agatatttat gggatctttc
      301 gaatgatctt tgcggctttt aacgtgagtt ttggtggagt tgctggattt gttcgtccaa
      361 tcattttacc gatggcattg gggaccatcg aatcaaaagg gttagaagtc catcctaagc
      421 atgaagaaga actaaaaggt atggcttcag ctatggaaaa tgtctgctgg ttctttggcc
      481 aagtactatt catcggagga gcaggggcgt tgctagtcca atcaacactg aaagatctag
      541 gatatgacgt aacattggga cagcttgctt tagtggaagt tccagtggct atcacggctt
      601 tagttgtggc aagtgtctac ttctatctta aagaaaagaa attagcgaag aaatattatc
      661 caggaaaggg gcaataagat ggactttttt acaaatcctg atattttatt aggcgataaa
      721 ttactggaga tcctttatat cgtgatggga ctcgtattga tttacactgg agtaagaaat
      781 ttaacagata aaaccaatcc tcatcgttat gggtcagcct ttttctggac ggtcttaggg
      841 gtagttattg caggaggaag atggcttccg gcaatcgtga acggtggctt gatctttgcg
      901 atgaccatac cagcaattgc taagaaagtc agcaaaggag aaagccggct tccatcaaaa
      961 gaatatatgg aaaaaatgtc agacaaacta ggaatgaaaa tttttattcc agctttgagt
     1021 atcggtgtct ttgcgatctt gtttgctcta tttaccaatt taggtgcttt agtcggcgtg
     1081 ggcgtcggtg tttttgcagc catgctaatc ttgatgttct tttctcgaga taatcgacca
     1141 agtacatttt tagatgatgc agcagatatg ctgggcacag tagggccgtt aagcatgttg
     1201 ccaatgcttt tagcttctct aggtgcggta tttacaagcg ctggagttgg gacagtgatt
     1261 tccaatgcag taggtacgat cgttccagaa ggaaatgtga acatcggcat catcatttac
     1321 gctcttggca tggtgatatt tacggtaatc atggggaatg catttgcggc catcaccgtg
     1381 atgacggttg ggatcggtgc accattcgta tttagtcatg gagcgaatcc agcgttaata
     1441 ggaatggttg cattgacttg cggtttttgt gggaccctgc tgacaccgat ggctgctaac
     1501 ttcaatatcg tgcctgtagc gatgctggag atgaaagaca aatacggagt gatcaaaaac
     1561 cagttgttta ttgcgttatt tatgctagtt ttccaaattg cttatatgat tctatttaaa
     1621 taatagaaga gaggggttgt gacagaataa ttgtcgcaac ctcttctaac attgtagtgg
     1681 aacactattt ttttataaga atatattaag tttgatctgt tatgttattt aaactattgt
     1741 ttcctttatt tacgctgttc aaggaaaatg gtatgataaa agataaccat atgttaaatc
     1801 aatcggacgg aggaacaaca gatgaaagta ctagtaacag gttttgatcc atttggtggg
     1861 gacaaagtga atccagccta tgaagcagta aaaaaaatgc cagatgaaat cagcggtgct
     1921 gaaatcatca aagtggaagt accgaccgta ttcgaaaaaa gcagtcaagt agtcaaagaa
     1981 gccatccaac agcaccaacc agatgtcgtg atctgtgtcg gacaagcagg tgggcggtca
     2041 gcagtctcgt tcgaacgtgt cgcaatcaat ctagcagaag caagaatccc tgataatgaa
     2101 ggaaatcagc cattcgatac ggctttagaa gaaaacggtc cagccgctta ctttacctca
     2161 ttgccaatca aagcaatgac aaaaaatgta caagatcatg gtctaccagc gtacatctct
     2221 tatacagcag gaacatttgt ctgcaatgat atcatgtatc gtttgcttca tttgatcgaa
     2281 acacaattcc cggcaattcg aggtgggttc atccacgtgc cattctcacc cgatcaagtg
     2341 atcgatcgtc cagtgggtac gccttctatg tctttagaag acattgcttc ttctttgact
     2401 tatgcagttg aagcagcagt agaaaataaa gaagatattt ccggaaatgc cggaacaact
     2461 cactaattta gcaagaaaca ctggctggtt gctaacgtta tgttagtagc cagctttttg
     2521 taattagaaa ggaaagaaac agccgtggaa gaatggcaat ccgtctttga agaatggttt
     2581 ccaaaagaaa tcagcaaaag ctatcccatc aaaatttcta aacaatacac aagcagtcaa
     2641 cgttgggaga tctatgcaaa attaacgaaa aaacagcggg aactagtcga taagcaccgc
     2701 cgatatctaa tcagctctcg ttttatggaa gaacattatt tagctgcaac agattgggtg
     2761 ttttctgact tcaagatcaa tccgtttttt cgaacaaaac gaagccagca aaaattgtat
     2821 tgtgagtgtg gtagagaatt aaaagtgcaa tacatcgtta aatcgccaaa aaccggaaag
     2881 atcctaaaac taggcatcaa ccactttgcc gatcacctgc atgtctctcc gacagtggca
     2941 gcttcgatcc atcagggaat gacaaaagtt gatctggcct tagacgaatt attatggctt
     3001 aagcagaaga atatcgattt tcctgaagga ctatggcaaa aatactgttt tgtcttgtac
     3061 caaaatcgtc ggatgaaaca gccatattta ccggatataa aattagctca gcggctcgca
     3121 gaatttcgcc aagtagaaat gccgatttac atagcggatt atcaagcatt ggaaaatgaa
     3181 atcaagaaaa taagtgaaca catcaatggg cagtcaaaaa agcgacagat aaaaaaagaa
     3241 ctatttgacg actttgcaga agaattagta aaggatgtag aagaattctt gatcaactat
     3301 cgcgcatttt tacgtaaaga ttggcaatcc atcgtatatg aagaagtgcc agtacatcct
     3361 aatgcttatt ttgaaacatt tatttccgtg ttgagaaaga caaaacgtca acgaacacca
     3421 gaagtcactg ctcagatgga atattttgcg aaaaaccaac gattcatcca accaaaaatc
     3481 tatctattta tttggaagca atattgtcgc tatggtttta cagaagggtt cttcgacagc
     3541 attcctcgga tagtcagaaa tggtttttta aaagtattgc gtaaagaacg agaagctata
     3601 caatctgctg ataagaaaga ccgtacagtc tcaaaagaaa aatggcaact agtagtgaaa
     3661 gatatccaat ctggaaatgt gcaagagaca atcgacaaat ggaaaggaaa acattatcgt
     3721 tttactgaag ctcagaaaca agcgttggaa tattatcaga agttagaaga atcactgcgt
     3781 ttcaatgatg aggcaagaaa gtatctgaag gaattactat aaaaaacata ctaagattag
     3841 aggggaacat cattgacgaa tgatgttctc ctctattttc tatttttaga ttatagtgaa
     3901 gagtgaaaga aagttcgaac aaagccttga ggactacgag tccctatcaa aaaccgaact
     3961 tctttactgt cattttagaa tcaggtttac gtatgttggg gccatgagcc cgtgtatagg
     4021 aaattggaat cgaaatgaag cagtaaagtt ctttcaaaac ttaaaaagga ggaaaacaaa
     4081 tgccttacat tcttgcatta gatgtttcaa tgggacacag ctacagtgtc ctatacaaag
     4141 acgacacctg cttatttgaa gacgagattg aacacactca aagaggattt catgcattac
     4201 tcgaagagat ccagcacttg cctgagcgtc cattgattgt ttttgaagca acggggattt
     4261 attccagacc agtcgaaaag ttctttcaag atcatcactt ttcttactgt ttgttaaacc
     4321 cgttagaggc gaaaaaacag atggaagaaa gcaccttacg cagttggaaa acagataaat
     4381 cggatgccca ccggctcgca cagactcatc ttaaaaatca acgtcagcca aaagaagtac
     4441 aaagcaattt ttacttagag atgcgcgatt tagcccgttt ttatcaagaa attgagaaga
     4501 aaatcactcg tctgcgcatg gatttacaca actgccttca attgactttt ccagagcttg
     4561 aacagttctt ttcaaacagg ctgacccctt acgcattgac attgatccgt ctttttcccc
     4621 atccagattt tgtattggca tctactcgga caaaaataaa aaataaactg atcaacgaaa
     4681 cacggaaaaa aatctccgcg aatcgtgccg agcaaaaggc agatcaaatc atccactatg
     4741 cacagtgtgc ctatccagct gttgaaaaag acagtatcca ctgtcaaaaa acgatctatt
     4801 acgccgaact tctccaagat ttactcgaac aaaaaaaagc actggcgaca caaatgatca
     4861 aaaaagccga aggctctcct tgctttttgt tgtaccaaac gttccctggg attggcgcac
     4921 tcacagccgc tttattgctc ggtgaattag gagatatcac acgatttaaa acccataaac
     4981 aattgaatgc ctttgtcgga atcgacatcc gacggtatca ttcaggtaag tatactggcc
     5041 aagaccgcat caataagcgt ggtaatccca aagcacggaa aattattttc ttcaccattc
     5101 gaaatatgat tcgccagcaa cgcgcagctc ctaatcatat tgtcgattat tactacaaat
     5161 taaaaaagca acctatccct aagaaagata aggttgccac agttgcttgt atgaacaagc
     5221 ttctgaaatg tatgcacgct atggttaggg cacatacaga gtacgactat gcgtacgcgg
     5281 tctcagtgga ccattaacta aaccacttac agtataccac ttctttgccc agatcgaaaa
     5341 ttttttacga acggggcttt atttggtgtg ctttgatttt gtcgctttcg tttacgagct
     5401 atttttcttg caccttttac ttgactaatc gtaggaaata gaggctggga cagaagcgtt
     5461 taactccaag aaataagaag aaattcacga aaattgcttt tcaaattttt gtgaatttca
     5521 gcttatttcc gaaggagttg cttctgattc cgccgtttat acgtgtttag agcgtgagac
     5581 aaaagtgttc tttacttttg tcccacgctc agttttttta tgcgtgttgt tgtgcgttgt
     5641 agtattcttc ttccattttt tgcatacgtt cacgttcttt ttggttatac caaggtgaaa
     5701 cggtaaatgc aaggacatca ttcagcaagt aaacagagct gttcacgaac atcgccaaac
     5761 ttgcagatcc atgcgtaaat gaaatatacc aaaggaccat ttgagcaagc cctgaagcta
     5821 accaccagta atattggttg ttgtaacgca agaaactgat gataccagca gtcaagctaa
     5881 tagaaaaagc aatggcatct acccacggcc gaggatcatc tgtataacga ccaatcagga
     5941 atcctgaaac aagatagaca aggaaagtac tgatgattgc gataaaccag ctttttccag
     6001 tgaattttcg gatttttgaa gccatattat gattccattc tttacttaac aatacgggga
     6061 tatccaatgt gatcatataa gcaatctgtt caccgatact taaatagttc tttgctgaaa
     6121 acccaacgat aataaaacaa atcgcagaca aaatacctaa aataccgttg actgattttg
     6181 ccgcattgat ggataaaata cacaatacac caagcgtggt accgataaat gtaataacgg
     6241 acagccaagt gatgggacta ttgacaagag tcatgacctg gcagccaaga ctgaaaaaga
     6301 aaagataata attttgttgc ggccagcctt ttagctgatg agctaaaaac tgaaaatatg
     6361 acttcatgta tgaggactcc ttaagacact aaatgtagtg tgttctgtat tta
//
LOCUS       DAJSKS010000137         6319 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJSKS010000137 DAJSKS010000000
VERSION     DAJSKS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.141"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(99..1229)
                     /locus_tag="ON396_002386"
     CDS             complement(99..1229)
                     /locus_tag="ON396_002386"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR3114441.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ON396_002387"
     CDS             complement(1878..3080)
                     /locus_tag="ON396_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3114442.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ON396_002388"
     CDS             complement(3140..3343)
                     /locus_tag="ON396_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HCR3114443.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="ON396_002389"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="ON396_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="ON396_002390"
     CDS             4880..5692
                     /locus_tag="ON396_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114444.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1877 a         1299 c          996 g         2147 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaat
//
LOCUS       DAJSKS010000138         6227 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJSKS010000138 DAJSKS010000000
VERSION     DAJSKS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.142"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(10..879)
                     /locus_tag="ON396_002391"
     CDS             complement(10..879)
                     /locus_tag="ON396_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HCR3114445.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            1032..2492
                     /gene="rhaB"
                     /locus_tag="ON396_002392"
     CDS             1032..2492
                     /gene="rhaB"
                     /locus_tag="ON396_002392"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR3114446.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            2489..3745
                     /gene="rhaA"
                     /locus_tag="ON396_002393"
     CDS             2489..3745
                     /gene="rhaA"
                     /locus_tag="ON396_002393"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR3114447.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            3759..4604
                     /gene="rhaD"
                     /locus_tag="ON396_002394"
     CDS             3759..4604
                     /gene="rhaD"
                     /locus_tag="ON396_002394"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR3114448.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            4606..4917
                     /gene="rhaM"
                     /locus_tag="ON396_002395"
     CDS             4606..4917
                     /gene="rhaM"
                     /locus_tag="ON396_002395"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR3114449.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            4999..6150
                     /gene="fucO"
                     /locus_tag="ON396_002396"
     CDS             4999..6150
                     /gene="fucO"
                     /locus_tag="ON396_002396"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR3114450.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
BASE COUNT         2012 a         1053 c         1451 g         1711 t
ORIGIN      
        1 ttatatgaat cagctgaatt tacgaaagtc tctcggcgat agtcctgttt ttgctttgaa
       61 caggcggcta aagtaagcaa aattctcaat ccctatttct tcggaaatca ttcgaaccgt
      121 aaattctgta taccgaagaa ggtctttagc gtaagaaagt tttttgctga tcaaataatc
      181 gattggtgga gaaccgataa atttggaaaa atctttgctc agctgatatt tattcaataa
      241 gaattgttgt tctaattgat caagtgtaat cgtttctttg aaatgttcgt ctaagtattg
      301 cttcagtttg taaacgtagg cgggaatatc ctcttcttcg aaatctagct gatacttttg
      361 caaaatcagt tcattcagca actcgtggat agctacagag gactggaaat cggtacgggc
      421 attcgtgcct tgttgcatgt ggatcaattc tgtgatcaaa tggtggatct tgttttctga
      481 tggatcccct gttgaatgga aggtgttatg gccgtctttt gtgaactcct gataaaatga
      541 ttggacgttc tgaccattca aatggatcca atccatttcc catggttctt cgctgatcgt
      601 ttgataacgc tgatattctt ggcagtcaat aaaaaataag tctccagctt ttagctgttg
      661 ctgattatca ttatatgtaa gcgaaccttt gccagacaaa gtaaacttta tcaagtaaga
      721 aggtaaatgt gctcgttcag tatagtaagg gattggtgct ttgaagtaac cgatttcttg
      781 gatataaaaa aataaattcc tgcttctttg tgtaggcgtc aagatcactc tttttgagtc
      841 ttccgaccat gagtggttgg ctttttccca gtaatccatg taaacactcc caacttgtca
      901 agataatgca attatatacc aaaatagtgt gaagcaaaat ataagattct gtattatgat
      961 agcattgtta ataaatgata acgctttatt aataaagtgc taatcaacaa taaagtaaaa
     1021 gaggtggaga aatgaatcca acatttgttg ccatcgatat cggtgcatca agcggacgac
     1081 taatgaaaag tacgagggat gcaaaaggtt ctttgatctt ggaagaagta caccggttca
     1141 aaaacggctt tcggaacaaa gacggtcacg atcgctggaa gatcgattat cttatcaaag
     1201 agattttgat tggatttgaa aaattaaagc agacagggat tgatcgcctt tttgcgggga
     1261 tcgatacatg ggcagtcgat tactgtctga ttgatgaaca aggggatcgg ataaatgatc
     1321 cgattgctta tcgggacaag cgtaccaatg gagcagtaga acagttttct gaaatctatt
     1381 ctctagataa gttgtatgag cagacaggga ttcagatcca gcctttcaat accttgttcc
     1441 aattgtttgc tgaagaaaaa gaaaatttgg caaaagcaca taaattgtta ctgatacctg
     1501 attatttagg gtatgtcttt actggtaaca tggtgacgga gaaaactaat gcatctacta
     1561 tgcagctgct gaatgcaggg acaaaagaat gggaaataga acttcttgaa aaaatcggtg
     1621 tctcgaagga attatttcca ccattgacag agccgggaac gatactagga gatctgcaaa
     1681 aagaacaatt ttcaaaatat gatttgccag atgtgacctt tatcacagta gcaagtcatg
     1741 atacggcgtc agctgtatta ggaacgccag gtcaaggaga acgttggggt tatatcagca
     1801 gtgggacatg gtcgctttta gggattgaaa caaagatcgc caatgtttct cgcaatgctt
     1861 tttctgaaaa ttatacgaat gaatggggtg cccataacac ggtgcgtttt ttgaaaaaca
     1921 tcatggggat gtggttgatc caggaagttg cccgaatgca agagtacgag tacagttatg
     1981 cggaattggc agagcttgct gcaaaagaaa aaccgctgca gcaagtgatg gaaatcaatg
     2041 accctcgatt tttgaatccc ccaaatatga tagaagagat tcagcaatac tgttttgaaa
     2101 cagagcagcc agtccctgtc tcatcgggag ctatcgctcg ttgtgtgtat gataatcttg
     2161 ctctttgtta cgcacaagag ctgatcaaag tagaacactt aactggaacg aaattggaaa
     2221 agctgcacat cgtagggggc ggatcgaata atcgtctact gaatcaattg acagcagatt
     2281 atgccggaat cattgtagaa gcaggaccta gtgaagccac agcaatcggg aatgttgcca
     2341 tgcaaatgct cagtacaggt cagttttcga atatagaaca agcaagaaat gtaatcaaac
     2401 agtcgtttcc atgtgaaact ttttacccag atgaaaataa gcaaaacacg atggaatggt
     2461 acgtgaataa aatcaaagga gtaatcgtat gaacacgatt gaaagaaatt acgaacaagc
     2521 aaaagaaaaa tatgcaacta tcggtgtaga tacagatgct gtactagaaa agatacagga
     2581 catcaaaatt tctatgcatt gctggcaagg agatgatgtc aaaggtttct tgacaccgga
     2641 tggcgaattg accggtggaa tcatggcgac agggaatttt ccaggagccg cacgaacacc
     2701 tgaagagctt agacaagatc tggagaaggc ttattcgttg ataccgggca aacacaaatt
     2761 gaaccttcat gctatctatt tggatacaga agaatctgtc gatctaaatg aaattgagcc
     2821 gaaacatttt gaaaaatggg tagagtgggc aaaaaaagaa gaaatcggtc ttgacttcaa
     2881 tccgactttc ttttcgcatc caatgatgaa agacggcttt acattggctc atccagataa
     2941 agaagttcgt gaatactgga tcgaacacgg gaaaagaagc cgcaaaatcg cggagtactt
     3001 tgggaaagaa actgggcaaa caagtatcaa taacttttgg gtacctgatg ggatgaaaga
     3061 taatccgatt gatcgtttta ccccacggga acgtttaatg gaatcactcg atgaaatttt
     3121 tacagaagag ttagatgaac ggtatgtaca agatgcagta gaaagcaaac tatttggttt
     3181 aggcgctgaa gcttatacag tcggttccca tgaattttat atgggttacg ggttgactcg
     3241 tgacaaactt gtttgtctag atgctggaca ttttcatcca acagaagtga tttccaataa
     3301 attatcttca ttagctttat tcagcaaagg aatcatgttg catgtttccc gtccagtgcg
     3361 ttgggattca gaccatgtgg ttttgatgga tgatgaattg caggaaatcg ctaaagaatt
     3421 agtccgtaac gaattgttgg aaaaaacaaa tatcggtctg gatttctttg atgcaacgat
     3481 caaccgtatc gctgcttggg tcatcggtac gagaaataca caaaaagcac tgctgaaagc
     3541 tatgctggaa ccagttgagc gattgaaaga aatggagctg gcttttgact ttacttcaag
     3601 aatggcttat acggaagaat tgaaagattt cccgtatgcc gatgtatgga attatttctg
     3661 ttacaaaaat caagttccag ttggtttgga ttggctggaa gaagtccagg aatatcaaaa
     3721 agacgtgttg gaattaagaa aataggagtg tggctatatt gaaaaaaata gatattttac
     3781 aagcgcctta tgtgaaagaa atgatcgaaa cgacgtcgaa tctttatcgc ttgggatggg
     3841 atgaaagaaa tggcgggaat atttcttatt tactaaaaga agaagagatc acaccgttct
     3901 tggatcctga aaaagtgatc cgccgcatct cgatgatttt tgatgccagc cgtttagcag
     3961 gatgctattt tattgtaact ggttcaggaa gatattttaa aaatgtagcg agcgaacctg
     4021 ccgagaatct tggaatcgtt cgagttgctg aagatggcaa aacactggaa cttttgtggg
     4081 ggctggaaaa cgaatgcctt cctacaagcg agttgccaag tcatttcatg agtcatatcg
     4141 cccgtttaga agtagaccca gataatcgga tcgttatgca ttgccatgct agtcatctat
     4201 tagcgatgag ttttacgcat gaattagacg aacgttcctt tagtcgtacg ctatggcaaa
     4261 tgtgtacgga atgcttagtt gtttttccag aaggaatttc catcattccg tggatggttc
     4321 cgggaacaaa tgagattggg gaagcaacag ctgaaaaaat gaaagaaact cgattggttt
     4381 tatggccaca acatggaatc tacgggtcag gaaaagacat ggatgaagtc tttggtttga
     4441 tcgagacagc agaaaaggca gcagaagtct atacgtacgt caaagcgcaa ggaccaatct
     4501 tgcaaacaat cacggacgaa aacctgtggc atttagcaga tgcgtttggt gtcactccaa
     4561 aagcaggtta tttggaggaa gttcatacga aagcaggtgt ctgacatggt gaaaaaagct
     4621 ttttgcatga aagtttaccc agaaaaacaa gaagaataca aaaaaagaca tgatgaactt
     4681 tggccagaaa tgcgtacagc acttcgtgaa cacggggtca tcgactattc gatctatctt
     4741 gatccgagta caagtttgtt atttggttac ttagtgatcg aagatgaaaa gttatgggaa
     4801 aaaatggcaa atacatctat caatcaaaaa tggtggaagt acatggaaga tatcatggag
     4861 acaaatccag atgcctctcc agtcagttgg gaattgatgc cagtctttga actgtagcaa
     4921 caagcaactg ggcaaccggt tgcttggggt tctttcgttt ttattgtgaa aaaaaataca
     4981 aattaggagg aatagaaaat gagtaatcga atgattttaa atgaaacatc gtattttgga
     5041 aaaggagcga ttgagtcgat cgtccctgaa tttcagcagc gaggattcgg caaagcagct
     5101 gtcattactg acaaaggatt gatcgagcac ggtatagcaa caaaagtaac agaacttcta
     5161 gataaagcag caatcgctta tgctttatac gacgggatcg tacctaatcc aacgatccaa
     5221 aatgtaaaag atggagtggc ttttgttaaa gaggcagagg cagactgttt gatcgcaatc
     5281 ggtggaggtt cgccaatcga tacggcaaaa gccattggga ttattttgac gaatccagag
     5341 ttttcagacg tcattagttt agaaggtgtt gctgatacga aaaatccatg tttacctata
     5401 ttagctatac caacgacttc tggaacagca gcagaagtaa cgatcaacta cgtcatcaca
     5461 gatgaagtaa atcaccgaaa atttgtttgt gttgatcctc atgatatccc aatcgtcgcg
     5521 ttcatcgaca gcgatatgat gatgggaatg ccgaaaaaat tggctgcatc aacaggtatg
     5581 gatgctatga cgcatgctat tgaagggttt atcacaaaag gcgcttggga aatgactgat
     5641 atgctgcatt taaaagcaat cgaaatcatc ggccattctt tggaagtttc tgtagatggc
     5701 gatcaaaatg ggcgagaaaa aatggcatta ggacagtata ttgcgggaat gggcttctca
     5761 aatgtcggtt taggactggt acacgggatg gctcacccgt taagtgcatg gtacaacatt
     5821 cctcatggcg tagcgtgtgc agcacttttg ccaactgtca tgaaatataa caaagaatat
     5881 actggagaaa agtaccgtga gatcgctctt gttctaggga ttaaaggagc agctgaaatg
     5941 tctttagaag atgtaagaga agcggcttgt ggtgagatcg atcggttgag caaagcggtt
     6001 ggaatcccag aaaccatttc tgaattaggt gtaaaagaag cagatattcc agcaattgca
     6061 gaagatgctt tgcgagatgt atgcacacca ggaaatccaa gagaaacgac agtagaagaa
     6121 atcattgctt tatatcaaag tttgatgtaa aaacgaagcg gatcttcaac attaaattgc
     6181 ctgtaaagag aaatggtagt ttatatctgg atatctcttt taaattg
//
LOCUS       DAJSKS010000139         6211 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJSKS010000139 DAJSKS010000000
VERSION     DAJSKS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.143"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..2103
                     /locus_tag="ON396_002397"
     CDS             <1..2103
                     /locus_tag="ON396_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR3114451.1"
                     /translation="NYLTHLYFGKKITHYSGGYRYPRTDRSFSPNNANAQDRLLSLDT
                     LPLEFPGYGHGDFREPAHNIKLGNGSRVNDFRYDSYEIQKGKPSLAGLPALYTEDDDE
                     AETLIIQLKDAISGLTLQLFYTIYQNHSVIIRSTQLVNNSDETVEINRLASQSIDFPN
                     RPLELIHLNGTWARERQMTREKIHTGIKVLDSKRGSSSHQQNPFVALVDAKTDEFQGE
                     AYGFNLVYSGNHETVIQKDPFDQTRVIMGINSFNFGWQLKTGDSFQTPEVVMVYSNEG
                     LNTLSQTYHELYNHRLIRGNYRLKERPTLINNWEATYFDFNEEKIMNIVDDAKNLGIE
                     MFVLDDGWFGGRENDFTSLGDWVETEGKLACGLENLAKQVHDNGMKFGIWFEPEMISE
                     DSELFRAHPDWALGIPGRDRSISRNQYVLDFSRQDVRDNIYQQMTAIFDRVPIDYVKW
                     DMNRNMTEVYSVLLDSTMQGEVAHRYMLGLYEFLEKLTTNYPTILFESCSGGGGRFDA
                     GFLYYMPQTWTSDNTDAVARLKIQYGTSLVYPISSMGAHVSAVPNHQTGRETSLTIRG
                     NAAMAGVFGYELDLTQLTSEEKAEIKEQVAFYKKHRQLLQYGTFYRLESPFEGNDTAW
                     MFVSKDQTEAMVFYFRVLAEAAHPLVTLKLAGLAEEATYQMEDVTFAGDELMNLGFYI
                     DSELHGDYATQRFYLKQI"
     gene            2164..3384
                     /locus_tag="ON396_002398"
     CDS             2164..3384
                     /locus_tag="ON396_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HCR3114452.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            3381..4841
                     /gene="gtfA"
                     /locus_tag="ON396_002399"
     CDS             3381..4841
                     /gene="gtfA"
                     /locus_tag="ON396_002399"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR3114453.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            4918..6024
                     /locus_tag="ON396_002400"
                     /pseudo
     CDS             4918..6024
                     /locus_tag="ON396_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         2131 a          996 c         1229 g         1855 t
ORIGIN      
        1 aattatttaa cgcatcttta ttttggaaaa aagattacgc actattctgg tgggtatcgc
       61 tacccccgaa cagatcgttc cttctcaccc aacaatgcaa atgcgcaaga tcgtttatta
      121 tctttagaca cattaccatt agaatttcca ggatatggac atggtgattt tagagaaccg
      181 gcgcataata ttaaattagg aaacggtagt cgtgtaaatg attttcgtta tgatagctac
      241 gaaattcaaa aaggaaagcc cagcttagcg ggattgccag ccttatatac ggaagatgat
      301 gatgaagctg aaacgctgat tattcaattg aaagacgcga ttagtggatt gactttacag
      361 ttgttctaca caatttatca aaatcacagt gtaattattc gttctacgca attagtaaat
      421 aatagtgatg aaacagtgga gattaaccgc ttagctagtc aatcaattga ttttccaaat
      481 cgtccactcg agttaattca tttaaatggt acctgggcaa gagaacgtca aatgactaga
      541 gaaaaaattc atacagggat aaaagtatta gatagtaaac gcggatcaag cagccatcaa
      601 caaaatccgt ttgttgcttt agtagatgcc aaaacagatg aatttcaagg agaggcatat
      661 ggatttaatt tagtttatag cggtaatcac gaaacggtga ttcaaaaaga tccatttgac
      721 caaacacggg tcattatggg aattaattca tttaattttg gttggcaact aaaaactggc
      781 gactcatttc aaacgccaga agtcgtgatg gtttatagta atgaaggtct gaatacgtta
      841 tcacagacgt atcatgaatt gtataatcac cgtttaattc gtggaaacta tcgtttaaaa
      901 gaacgtccca cgttaatcaa caactgggaa gcaacgtatt ttgattttaa cgaagaaaaa
      961 atcatgaata ttgtcgatga tgcgaagaac ttgggcattg aaatgtttgt tttagatgat
     1021 ggttggtttg gcggtcgtga aaatgacttc acttcattag gtgattgggt tgaaacagaa
     1081 ggcaaacttg cttgtggttt ggaaaattta gccaagcaag tgcatgataa cgggatgaaa
     1141 tttgggattt ggtttgaacc ggaaatgatt tctgaagata gtgaactatt tcgagcacat
     1201 ccagattggg cattgggtat tccaggtcgt gaccgctcaa ttagtcgaaa tcaatatgtt
     1261 cttgattttt caagacaaga tgttcgtgat aatatctatc aacaaatgac ggcgattttt
     1321 gatcgtgtac cgattgatta tgtgaagtgg gatatgaatc gaaatatgac agaagtctat
     1381 tctgtcttgt tggactcaac gatgcaaggt gaagtggctc atcgttatat gttagggtta
     1441 tatgaatttt tagaaaagtt aacaacgaat tatccaacta ttttatttga aagttgttcg
     1501 ggtggaggcg gtcgttttga tgcaggtttc ttgtattaca tgccacaaac ttggacgagc
     1561 gacaataccg atgccgtggc tcgtctgaaa attcaatatg gaaccagctt agtgtatcca
     1621 atttcaagta tgggtgcgca tgtttcagca gtcccaaatc atcaaacagg aagagaaacg
     1681 agtttaacta ttcgtggcaa tgctgcgatg gctggtgttt ttggttatga gctagatcta
     1741 acgcaattaa cttctgaaga aaaagcagaa attaaagaac aagttgcctt ttataaaaag
     1801 catcgccaac ttttgcaata tggaactttt tatcgcttgg aaagtccgtt tgaaggcaat
     1861 gatactgcat ggatgtttgt ctcaaaagac caaacagaag ccatggtctt ttatttccga
     1921 gtattagcag aagctgcgca tccattagtc acattgaaat tagcaggatt agcagaagaa
     1981 gcaacttatc aaatggaaga tgtaacattt gctggcgatg aattgatgaa tctcggtttt
     2041 tatattgact cagaactaca tggtgattat gcgacacaac gcttttattt gaaacagata
     2101 tagtagcttg aatttacaat ataagtgcaa caccaaaaga gactcattcc aaacgaagaa
     2161 aatatgtttt atttacacgc atttactaaa aagcagcctg atttgaattg ggaaaatgaa
     2221 gaagtacgtg aagaaatcta tgcaatgatt aactattggt tagataaagg actaggcgga
     2281 tttagaatcg atgcgatatt aaacatcaaa aaaaaactag agtatggcat ctttgaatct
     2341 gatggagaag atgggttagc cttcattggg ccctggattc ttaatcagtc gggcattgaa
     2401 gtgtggttaa aagaaatgag agagcggaca tttaaaccac ataatagtat gacggtagca
     2461 gaagccgatg ttcctaatga acgtttagat gaatatattg gtgaagatgg ctattattcg
     2521 atggtttttg attttagtta tacggatatt gatgtacctg aaactggtga atggtttaaa
     2581 gatagccaat ggacatggac ggatatgcgt accaatattt ttacgaatca attagttacg
     2641 caagataaag gttggggcgc cttatattta gaaaaccacg atcaaccacg ttcaattaat
     2701 aaatatattc cagaagaatg gatcaatgat tatagtaaaa aaatgttggc gacgcttttt
     2761 atgttactaa gaggtacccc ttttatttat caaggacaag aactaggaat gaccaacata
     2821 gaaatggcat cattagaaga ttttgatgat gtagctacgc attctcagta taaacgggca
     2881 ttagaatatg gattggcacc agagcaagca ttgaaagctg tcaataaacg aagtcgggat
     2941 aattctcgca cacctatgca atggaatacg caaaaaaatg ccagattctc aacttctgat
     3001 aatgtatggt taaaagtaaa tcctaattat ccagaactca atgcagaagc tcagttaacc
     3061 aatcaacagt cggtattcaa ttattatcat caattaatta aattaagaaa aaatagtgta
     3121 tacaaagacg tgctaatcta cggacaattt ctaccagtga aagataaaaa tgaacatatt
     3181 ttgctttatg aacgccaatt agaagataaa atcgtattag ttatcttaaa cgttacagca
     3241 acagaacaag aatattatgt agatgaacag taccaacaag tattaatcaa taattatgaa
     3301 gatgttttat tagatcaaag tcgaaaattg cgtttacgac cgtttgaaag tattgtatta
     3361 gctaattatg gaggacaatt atgaaaatta aaaacgaagc aatgctcatc acctatgcag
     3421 atagtttagg aaaaaacatg ggcgaattaa aacaagtctt agacaaacat ttacaaggtg
     3481 ttattggtgg tgtacatctg ttgccttttt ttccatcgac aggtgatcga ggctttgcgc
     3541 cgagtgatta tacgacagtt gatccagctc ttggaacatg ggaagatgtg gaagcattag
     3601 gtgaagatta ttatttgatg tttgatttta tgattaatca catttctcgt gaatcaaaat
     3661 ttttccaaga tttcaaaaaa aaccatgagc agtcaccata caaagaaatg tttatccgta
     3721 ttcatgaatt tttcccagaa aatcgaccaa cacaagaaga tattgattta atctacaagc
     3781 gtaaagacaa agcgccgttt caaacggtag aatttgcgga tggtacgaca gaagaggttt
     3841 ggaatacctt tggggaagaa caaattgatt tagatgtgac caaagaagtt gtgaaagaat
     3901 ttattcgaga aaccatcaaa gatatggcga accatggctg ttctttaatt cgattagatg
     3961 cgtttgctta tgcgattaaa aaattagata ccaatgattt cttcgtggaa ccagatattt
     4021 gggaactttt agacgaagtg cgtcaagaag cagcgaaata tgacgtcgaa ttattaccag
     4081 aaatccatga gcattattcc attcaaatga aaattgccaa tcatgattat tatatctatg
     4141 acttcgcctt gccaatggtc actctgtatt cactttatag tggaaaatca gaacgtttag
     4201 ccaattggtt gaaaatgagt ccgatgaagc agtttacaac attggatacg catgatggaa
     4261 ttggtgttgt ggacgctcgt gatttattaa cagatgaaga attggattac acctctgagg
     4321 aattgtataa agtcggggcg aatgtgaaga aggtctattc aagtgccaat tacaataatt
     4381 tagatattta tcaaattaac agcacctatt atagtgcatt aggcgataat gaccgtagct
     4441 atctgttagc acgtgcgatt caagtatttg cgccagggat tccacaaatt tattacgttg
     4501 ggttattagc aggaaaaaat gatattgaac tgttggaagc aacgaaagaa gggcgtaata
     4561 ttaatcgtca ttactatgat ttagccgaaa ttgaagaaga agtgcaacgg ccagtgattc
     4621 aacaattatt tgcactttta aaatttagaa atgaatctgc ggcgtttgat ttggatggaa
     4681 cgattgaagt agcaacgcca tcagaatcag aaatagtgat tacacgtaaa aataaaaacg
     4741 gtagtgatag tgcaacatta acggctaact tggatacgaa agaatttatc attgagtcta
     4801 aagatataaa aattttcact agtgttgcat taaaatttta acctattcca tagaacaaaa
     4861 aatcctttat actagcactt ggatgactta tccaagacca ataaaaagga ctcatacatg
     4921 gataagtata aaacaaatac agcatttaat aaatggtttt ctgccataaa attaggaaaa
     4981 ctttctgtca actcccaaaa atgtattttt gattacaatt cgtatcgtaa aaaactgtct
     5041 tttgaagctg tgctcaagct ctttctctac gcagttaatg aagaaaaaga aagtttgcgt
     5101 gatttgagta cagctcttat cgaccgatcg ctccaactag aaaccgatgt gtcatcgatt
     5161 agtcataccc aattgtctcg agcatttaat gcacttgaac ctaaagtgtt agaagaaatt
     5221 tttcaacaac tgttagaaaa agttcaacat caaatcaaac caaccaagcg aaatcatctg
     5281 tatctgattg attccagtac gttttcgctt agtttaaaac gtcataagtg ggcgactttc
     5341 cgtcaaacaa aatcaggtgt caagttacac ttaaactatt gttatatgga cgatactacg
     5401 atgtatccaa ctgattttac cttaacaaat gcccatgaac atgatctcaa tcaactacca
     5461 gtattggtga atcaaccaga ggcaacttac gtgtttgatc gtgggtatta ggactttgaa
     5521 aagatggatc agatgcattg ggatggctat ttcttcgtca ctcggattaa aaaaaacacc
     5581 aaagttcacg tgatagatac tctggaaaca tcgccagaaa cagaaattct acgagacgag
     5641 ctggttcgtt taggatctaa aacctatcta accgccaatt tccgactagt cactgttcaa
     5701 gataagaacg gacgtgtgtt tcaatttatc accaatcgaa tggacgtctc ttcaaaagaa
     5761 atctctgata tgtatcacgc tcgttggcaa atcgaattgt ttttcaagca catcaaacaa
     5821 cacatgacca ttaaaacctt cttttctcaa agtgaaaaag gcgtgcagaa tcaattgatt
     5881 ttaacgatga tttccgctct cctcacgttt ctcatcaaat tggagacgaa aacggaaaaa
     5941 tcagtgttcc aaatcaaacg attctttcgt tatttactgt ttcagccctt tgaatgttgt
     6001 ctagaaaaat taattcccac atgagaaaaa agaaatttgg ctcgttactt ttggttttca
     6061 gttgaataac agataaaaaa ttttctggaa aaagtcatcc tctgcgtcag cacagttttt
     6121 tttgacttat tttcagagga aatagttttc agaatattct aaaatgtttt atgcaacact
     6181 agtgatattt tttagaatta gatttctaat g
//
LOCUS       DAJSKS010000140         6187 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJSKS010000140 DAJSKS010000000
VERSION     DAJSKS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.144"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..465
                     /locus_tag="ON396_002401"
     CDS             <1..465
                     /locus_tag="ON396_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HCR3114454.1"
                     /translation="IGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            481..630
                     /locus_tag="ON396_002402"
     CDS             481..630
                     /locus_tag="ON396_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114455.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            704..1297
                     /locus_tag="ON396_002403"
     CDS             704..1297
                     /locus_tag="ON396_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCR3114456.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            1313..2212
                     /locus_tag="ON396_002404"
     CDS             1313..2212
                     /locus_tag="ON396_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCR3114457.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            2215..2700
                     /gene="ssb"
                     /locus_tag="ON396_002405"
     CDS             2215..2700
                     /gene="ssb"
                     /locus_tag="ON396_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR3114458.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            3066..3326
                     /locus_tag="ON396_002406"
     CDS             3066..3326
                     /locus_tag="ON396_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114459.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            3767..3973
                     /locus_tag="ON396_002407"
     CDS             3767..3973
                     /locus_tag="ON396_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCR3114460.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            3973..4224
                     /locus_tag="ON396_002408"
     CDS             3973..4224
                     /locus_tag="ON396_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114461.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            4239..4676
                     /locus_tag="ON396_002409"
     CDS             4239..4676
                     /locus_tag="ON396_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114462.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            4669..5376
                     /locus_tag="ON396_002410"
     CDS             4669..5376
                     /locus_tag="ON396_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114463.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            5757..5936
                     /locus_tag="ON396_002411"
     CDS             5757..5936
                     /locus_tag="ON396_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114464.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
BASE COUNT         2254 a         1032 c         1222 g         1679 t
ORIGIN      
        1 atcggtgaac gaaaaggaac acaaaccact tttctagcca atatccaaaa atttgtctct
       61 cacttattgg aagtcgtacc agggcaaatc caatccactg attttggatc gacgttacaa
      121 gaagtgaaag cagccagtga aaagcaagaa gccgcgcgtc atttaggaat ttgtccaaaa
      181 tgccaggaac aagaagtctt actctatcaa aaggtggcag catgtacgtc tgaagcctgt
      241 gatttcaaac tatggacaac gattgccaaa aagaaactta cagcgacaca gctgaaagag
      301 atcattcaaa atagccgaac aagccaacca gtgaaagggt taaaaggtca gaagggttcg
      361 tttgaagcaa cgattgtttt aaaagaagac ttcactacag gctttgaatt ttctgaaaag
      421 aaaaaaacga acttcaagaa aagaacacga cgcactacaa aataatggag ggttaatctt
      481 atgcgtaata caacacacaa acaacaagaa atcgaaaatc aaaaagccaa aattcgttca
      541 gaacaggaaa aaatgcgtgc cagcaaacga agactgcagc aattgaagaa ccagcagcgg
      601 attcaaaaag aaatcgaatt agatcgataa attcctttct tgttaatata aaactaacat
      661 gttatagttg tgttagtttt atattaactt taggggaaga gtaatgaaag acaaaatcca
      721 atgtattttt gataaacata atggcaacct atccttgaag gatgctagaa gtgaaggatt
      781 atcgcctatg acgttagatc gattagccga aaaggataaa atagaaaagg tagcaccagg
      841 tttctatacc ttaaaggatg cgttcgtgga tgaattgtat ctcgctcaga gtatctattc
      901 cagagggatt ttttctcatg agactgcgct cgatcttttt caagtaggaa catatatccc
      961 taaaaagatc aatatgatgt ttcctaaagg atacaatgta tcaaaggaga aattagatca
     1021 gtatgccatt caaccgcatt acactaataa agaaaatttt gacctgggag tcacagaaac
     1081 agaaagcttt tacggcaata aaattttagt ctatgattta gagcgaacgt tatgtgacat
     1141 gtggaatcca agatacaaag caagcgtaga ggtaaaacaa gaagcattaa aagaatatat
     1201 gacaagtacc agtagaaata caaataagct aagaagatac atggatattt taaaatcacc
     1261 aaaagaaatg actttgtata tgttgccact ttattgagag gagtaaagat gaatgacacc
     1321 gacacaattt aaagagaaaa caagaaattt cgcagcagag aaacatttaa acccacaaat
     1381 tgtccaaaga tattatatga tggaaaaatt attagaatgt attgttgaat cgccttatca
     1441 tgatgatttt gtattaaaag ggggatttct gattggatca aaatacgggt tagaaaaccg
     1501 ttcaaccgtt gatattgata cgacttatcg aaattcgaaa ctaacaaaag aaaagttaga
     1561 aactgttttt tctgaattaa cagctaatcc tacaaaagat ggaattcgtt ttgaattaaa
     1621 gggattaaca gagacgaggg aagccgatta ttatccaggt tttcaagcaa agtttattgc
     1681 ttatcttgaa aatgcacgtg taccttttaa attagatatt acttcaggtg attctattat
     1741 tccagcagca ttgatgtatg aacacaaatt aatgtttgaa gataaaacga ttcatattcc
     1801 agcttatcct acggaacaaa ttctagctga aaaattacat gccacgttta gttttggaaa
     1861 agataattcg cgcatgaaag atttttatga cctgtatatg attccgaaat tagaacgtat
     1921 cgatgagaaa aatttgtata agagtgttca aggaacattc aaggaaagaa ataatcaatc
     1981 ggtgtttaag catttgtatg atcaggaaat gcctcaattg gtgaaagatg agggcttgag
     2041 agaaaagtgg aaaagatacc aacaagaaaa ctattttgcc aaagacattt ctttcgaatc
     2101 tacactaact tccgttgatt ccctgatgca atcaattgta gagcaagaga aggcagaacg
     2161 aaaacaaaat tttaagagat cacaacaaat gaatcaagaa atggagagat aaagatgatc
     2221 aataatgtaa ctttagtagg gcgattaact aaagatccag atttacgtta taccgcaagt
     2281 ggcacagcag tggcgacttt ttccctagcc gttaatcgta atttcacgaa tcaaaacggc
     2341 aatcgagaag ctgattttat caattgcgtg atttggcgca aaccagcaga aacgatggcg
     2401 acattagcta aaaaaggcag cttgatcggc gttgtcggac gaattcaaac acggacttat
     2461 gacaaccagc aaggacaacg tgtgtatgtg acagaagttg tggccgataa cttccaacta
     2521 ttagaaagta aagcagcgac agaaagccgt atgcacgctg atcaatcgtc cagcagtcca
     2581 agccctacca cctttgaaca acgggataca gcgacgccta acaacaatgg attgaacgct
     2641 tcacagaatc catttggtgg acaatcaatc gatattagcg atgatgatct accgttctaa
     2701 gtagcatttt ctattggttg catttgctac ggtttatgtt ataatttaga caacaaaaag
     2761 aattttcaat ctataaccaa aaaccactag ctggcgggct agtggctttc ttttacgttt
     2821 ctattataag aattaacggg taggcttact gtctttcttg gcaaatttac aatcaagcca
     2881 tttgcagata tagtgcgcgg ctacttctgc catgattgca aaaacaaaat caaaaagaat
     2941 tttcaaacct ataacctcct ccccttgcca gattaggccg gggtagcaga cagcaagact
     3001 attatagcat aattgaaata tccaaatgat tggatttttg ctataatttt agcgaggtga
     3061 ttgtgatgaa caaatatgtt ggtttgcttg ataaaatccg agtaataaaa acgcaaccgt
     3121 tattggttcg ctttacttta caaacgattc atgaatcgat taattgtgta gttgcagata
     3181 ttgaaattat cgataaattg ctaattatgg atgatggaaa atacaatatt gccgtcacag
     3241 gtcacttcaa taaacgaaat cagctagtta ttgctagcat gtatgttaga aatcccgatc
     3301 attttacgcg atcgatgggc atataaataa aagaagagtt gtagtcctaa atgaggattg
     3361 cagctctttt tgttttacat agaaacagga aaagaccttt ttaggaagac acaagtgcct
     3421 tctcaattga aaaacctttc tctcactcca accgccaccg ccgttcgaga agaaaggaag
     3481 tgaacgctgc acgttcacta agatatggtg tcttgcaaag caagacaaaa tagacaaaac
     3541 ttcctctcac caaaaggttc gttcctgttc gttatctctt tattgatttg ccagaccggc
     3601 aacaagttgc cttttatcac caaaaagcga caaggacaag ggtaaatggc acgctgcgcg
     3661 cccttgtcct tgtcgctttt cgttgattta ttctggccaa caaatcaata aagaaagaac
     3721 taacgaaagg aagtaaaacc tttcctaaga ggaggcagaa tcaaacatgc gaaagaaatt
     3781 tgattattgg ggtgttccct tcagcgaatt attcccaaac tatcatgcac cacatactgt
     3841 tgaatgcgat tgtggagaaa gagcgaaatg tatcaaaagc tatcgtttgt atcaatgtcc
     3901 aacatgcggg aaaaaatata ccttgtctta tggcgattac attttgattg atgagaaagg
     3961 aaagaaacgc tgatgaaaac acaacacaaa ttattgactg taaaagaagt cgttcaaatt
     4021 ttggtggaca aagaagtcat ttctgaaaaa gtagcagaag ctttacttat tccgcggtac
     4081 tggacgatag aaaccccttt agtagaggat gcaccgacta tgctatttga agacctagtg
     4141 aagattgcag gatatgaact gcattatcaa gaaaacatgg aaacttatga aatcgaatat
     4201 tggttaacca aattagagaa ctaaaaagga gaaaaaatat ggctcaaaat tactatgacg
     4261 aatttgtcaa attacctttg gataaaatgg cacaaaaaat ggaggatatg acgttcctat
     4321 accatgaaac acgtgtaccc aaaaaacatt acaaggaaaa attgtcagtc gcagtggaag
     4381 aaatgattga atcaggtgtc gaaatgaatc tcattgcgac gtactatcga acactggaag
     4441 aactgaaaaa acaaaatgga aaatggtttt tccaagcctt actatgcttg gaagtaggtg
     4501 tcaaaccaag cactatcaaa ccttccgaat atcaagcctt agaactgact tatgcaaaat
     4561 tcgcacagat taagaaagct aaagcaatgg ctagcgactg gctagatact tttgaaagta
     4621 tcgaaaaaaa tggtgcattt tatacgattg aatcggagga gaaagataat gaataaggtg
     4681 gtttatcagc taacaacaga agatgtttcg caatttttaa caagcaaaga acacttgaat
     4741 gaagtaatca gacggattca aaaagacttt agttgtatgg aagaaatgaa gtactttatt
     4801 caaagagtgg aagaacagtt gtatatggaa agcgaaacaa gtcaaacgaa ggtcacagtc
     4861 agccataatc atttagacgt gtataaacgt cgtcctgaaa cggtaaaaaa aggaacagag
     4921 cttctacgac tagaaacaga aaaatacatc atgaatgtgt acctagaaag gagtgagctg
     4981 gtcgaaaact ttgtcagcta cacgatttgt aggaaacact attactcaga atcagaatat
     5041 tattcaattt tcgagagcat ggaattaatg gaagaagaaa tggaagagct aaacagcaaa
     5101 aatgcgacgg tagacgatta ttaccttgta tttactaggt ttttgacgtg tgggacggaa
     5161 tctatcgtgt taatgggaat ggattatgaa ctcgctgtac ccgaagcatt tttgagggaa
     5221 tatgtagaag aagtagataa cccaggcgac gactggtaca cgactttaac tgatttttta
     5281 gacacctaca cttacgacga tacagaagaa atccaaaatg aacttgaccg taaacgaatg
     5341 ccatatcaac gatttgaaag gaaaaaaggt aaataaccat ttataccgaa aagtgaggat
     5401 gtgtgaaata agaacaaaaa accttgaaat taagggaatg gttcttattt cgcaccaatc
     5461 gctccaaccg cccccgccgc ttaggtggtg cgaaataagc gggcaaagcc cgataaccta
     5521 agagaaagaa ggagatccat atgaataaaa tcattcctct attgttcgta ctgtttgttg
     5581 gcgagatact tatgcgtcat gctgcagctg tacatttaaa cttcgtagta ttcttaggaa
     5641 tactggtatt ttgtttgatc ttttggcgat ttgtattagc ccttgtgagt ctactattca
     5701 agatgattgc agccatattt ttcatagggt tatttcgatg aaaggagaga gtactaatgc
     5761 caattgcaat aggaaataaa cggttacccg tgacattaga tgaaaagaga caaaaagaat
     5821 tgcagcaact aaagcagaag tacggaaaaa gtgaatctaa gattatgtgt attgcgttag
     5881 atctattgat cgcgcaagaa aaagcaggat ttgatatacc agcactaaaa aagtgacgtc
     5941 acttttcatt tgaagaaaca aaagtgatag cacttaattg ttcctactat ttagaatatt
     6001 ttctatgtcc tttttattcc attttgtatc aataactatg aaatctttaa agtatccaga
     6061 agagattatt tttagatcaa tttttttctt acctgattct aaagattttg ttttcttaaa
     6121 catattatga tttcctttcg actattttca tataaatgtt aacatagttt atggattcat
     6181 ggaatgt
//
LOCUS       DAJSKS010000141         6173 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJSKS010000141 DAJSKS010000000
VERSION     DAJSKS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.145"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            725..811
                     /locus_tag="ON396_002412"
     CDS             725..811
                     /locus_tag="ON396_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCR3114465.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ON396_002413"
     CDS             complement(932..1621)
                     /locus_tag="ON396_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114466.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="ON396_002414"
     CDS             complement(1866..2780)
                     /locus_tag="ON396_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR3114467.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ON396_002415"
     CDS             complement(2762..3142)
                     /locus_tag="ON396_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR3114468.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ON396_002416"
     CDS             complement(3592..3879)
                     /locus_tag="ON396_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR3114469.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ON396_002417"
     CDS             complement(3899..4123)
                     /locus_tag="ON396_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HCR3114470.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ON396_002418"
     CDS             complement(4578..4748)
                     /locus_tag="ON396_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114471.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ON396_002419"
     CDS             complement(4763..5296)
                     /locus_tag="ON396_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCR3114472.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="ON396_002420"
     CDS             complement(5317..6159)
                     /locus_tag="ON396_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR3114473.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAJSKS010000142         6136 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJSKS010000142 DAJSKS010000000
VERSION     DAJSKS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.146"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            88..2529
                     /locus_tag="ON396_002421"
     CDS             88..2529
                     /locus_tag="ON396_002421"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCR3114474.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2859..3563)
                     /locus_tag="ON396_002422"
     CDS             complement(2859..3563)
                     /locus_tag="ON396_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114475.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3750..3995)
                     /locus_tag="ON396_002423"
     CDS             complement(3750..3995)
                     /locus_tag="ON396_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114476.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4012..4863)
                     /locus_tag="ON396_002424"
     CDS             complement(4012..4863)
                     /locus_tag="ON396_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR3114477.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5029..6033
                     /locus_tag="ON396_002425"
     CDS             5029..6033
                     /locus_tag="ON396_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HCR3114478.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1968 a         1064 c         1299 g         1805 t
ORIGIN      
        1 aaatttaatc ttagaaaaaa ataactggaa caagagctat ttgtttttac agcaaagaat
       61 ggtatatttt tgtaggaggt ggcttacttg aaagaagtcg ttatgataga tgctgcaaga
      121 acgccgatag gaaagtatcg cggaagttta agtccattta ctgcagttga attaggcaca
      181 cttgtgacta aaggattatt agataaaaca aaactgaaaa aagataaaat cgatcaagta
      241 atatttggaa atgtcctgca agctggtaat ggtcaaaatg ttgccagaca gattgctttg
      301 aacagcggtc tccctgtaga tgtcccagca atgacgatca atgaagtttg cggatctggt
      361 atgaaagcag tgatcttagc acgccagctg atccaattag gtgaagcaga acttgtgata
      421 gccggcggaa cagagagtat gtctcaagca cctatgctga aacagtatca gtcagaaaca
      481 aatgaatatg gtgaaccaat ttccagtatg gtcaacgacg gattgactga cgcattttca
      541 aatgcacata tgggattaac cgcagagaat gttgcaacac aattttctgt gagcagagaa
      601 gagcaggatc gctatgcctt gtcgtcccag ttgaaagcag cacatgctgt cgaagccggt
      661 gtattttctg aggagatcat cccagtcaag atttctgatg aagacgtgtt atctgaggat
      721 gaagcagttc gtggaaatag tacattggaa aaactgggca cgttacgtac agtattctca
      781 gaagaaggaa ctgtaacagc aggaaatgct tccccgttga atgacggtgc ctctgtggtg
      841 atccttgcat ccaaagaata cgcagaaaat aataatctgc cttatttagc aaccatcaaa
      901 gaagtagcgg aggtcggtat tgacccaagt atcatgggaa tcgctccaat caaagccatt
      961 caaaaattga cagatagatc aggaatgaat ttatcaacaa tcgatctttt tgagatcaat
     1021 gaagcatttg cagcttcttc tatcgtagtt agccaagaac tgcagttaga tgaagaaaag
     1081 gtcaatatat atggaggagc aatcgcatta ggccatccaa ttggtgcaag cggtgcacgt
     1141 atcttgacta cattagctta cggattgctt cgtgaacaga agagatacgg tattgcttca
     1201 ttatgtattg gtggaggttt aggcttggca gtcttgttag aagcgaatat ggagcaaaca
     1261 cataaggatg tgcaaaaaaa aaagttttat cagcttacgc catctgaacg gcgcagtcaa
     1321 ttaatagaaa aaaatgtatt gacacaagaa actgcgttga tttttcagga acaaacactt
     1381 tctgaagagc tctccgatca catgatcgaa aaccaagtga gtgaagtgga aatcccaatg
     1441 gggatcgcgc agaattttca aatcaatggc aagaaaaaat ggattccgat ggcaacagag
     1501 gaaccatctg tcattgcagc tgcaagtaat ggtgcaaaaa tctgtggaaa tatctgcgca
     1561 gaaacacctc agcggctgat gcgagggcaa atcgttctat caggaaaatc ggagtatcaa
     1621 gcagtgatca atgcagttaa tcatcgaaaa gaagagctga ttctttgtgc aaatgaaagt
     1681 tatccttcaa tcgtgaaacg cgggggcggt gttcaagata tctctactcg ggaattcatg
     1741 gggagtttcc atgcatactt atctattgat tttttagtgg atgtaaaaga tgcaatgggc
     1801 gctaacatga tcaactcgat tcttgagagt gtggcaaaca agttacggga gtggttccct
     1861 gaagaagaga ttctattcag tattttaagt aattttgcta cagaatcatt ggcttctgcc
     1921 tgttgtgaaa tcccattcga acgcttaggg agaaacaaag aaatcggtga acaaattgcc
     1981 aaaaaaatcc aacaggcagg ggaatatgcg aagcttgacc cttaccgtgc agcaacgcac
     2041 aacaaaggga tcatgaatgg tatagaagct gtagtggcag ctacaggaaa tgatacacgt
     2101 gcggttagcg cttcgatcca cgcctacgcg gcaagaaatg gattatacca aggattaaca
     2161 gactggcaga tcaaagggga taaactagtt ggaaaactaa cggtgccttt ggctgttgcc
     2221 acagtaggcg gagcttccaa tatcctgcca aaagcaaaag catctttagc tatgttagat
     2281 attgattcag ccaaggaact agcacaagta attgctgcag tagggttagc tcaaaacctg
     2341 gctgctttgc gtgcattagt tacagaagga atccaaaaag gccacatggg acttcaagct
     2401 cgttcattag cgatctcaat tggggctatt ggtgaggaaa tcgagcaagt tgcgaaaaaa
     2461 ttacgtgaag cagaaaagat gaaccagcag acagctatac aaatattgga aaaaatccgg
     2521 gaaaaataaa aaagcaatat ctttagaaga ataaagcaag atttacagtt attttattta
     2581 ctgcagatct tgcttttttc tctatcagaa aatatgttta ttattagaat tttttatagt
     2641 taaggttagg catcttaaga aatcaggcaa ctccaagata tcttcaggta gaagagaagt
     2701 cgcctaacat tgtaggatca taggtgattt gttggacagg tcaagctgat atttgagcct
     2761 gatgttagaa ggaatttctt tttacttgct gtagaaatta ttatatgact aaaaaggctg
     2821 ggattttaaa tcccagcctt ttatgacaaa tagaattttt atcgatcagt aatcgctgtc
     2881 gctgcaaatt gttcgatcaa aggaatcaaa gtcaatttga ctttgtattt ccgttcgagt
     2941 gctttttcga taacgaactc attttcagta gtcatagaaa tagttgctaa tttacgaggt
     3001 tcatcagcat ttttttcatt aataactact tcatgttcat tttgttctgt gtaaaattta
     3061 ggatactcag caacggcttt tgaaaaatca gcaaaactta aagcggcatt cgtatcagtt
     3121 gagcgtgtat tttcagttgt gtccgctttt ggtttttccg ttttttcttc tggcacactg
     3181 actgtttctt ctgtaactgg tgtcgcaaca gcttgcgcag ttggttcatc gtttttcaat
     3241 cgttgatcat tccagtaaga cacgatttca ataagaagat tgtcacggaa tcgttcgaaa
     3301 atcccgtttt ctcctgaacg aattctcttt aatacagtat tgaagttgtc atcgattggc
     3361 acgttgatcc gtgtatcttc atttgttttc ccagctaatc cgatcgttaa gatttgacgg
     3421 tttttgggat caagcatgac ttgtaacggc aaaccataac gtcgcaagct ctcttcttct
     3481 agagaacgga ataattgtcc tcttacacca tccaatgaag gacggaattg atttaataat
     3541 ttttgtttct gttttttctt catatgttct ctccttcgct catacccttt cgattataag
     3601 aaaaaaacag ccaaatgaaa aggagtaatg caaaaaaact tgcgtatacg caaattacaa
     3661 cagaaaaatt tgcgtatttt cccgtttttt gaaagaaaaa taaaaaatgg aacggattat
     3721 gtatatatac ataatccgtt ccattctttc tattttttcg gtatcaaggc aaaaacaggt
     3781 agttcaggtg aataaatgac ttcatgttcg tgccagggtt tgaccattag cgaatgatcg
     3841 aaccgttttt tcagttcttc gatgatttga tcatgtgtcc agtttcttgc aggaaaatgt
     3901 tggattttct ctgcatcgat caaaaagaca aaatcggaat gaaaagtctg ggtaacttct
     3961 tccgagagag aattcaagcg ttcgattgga tccataaaat cacctttcga cttaaaattg
     4021 ggtagtatct gggtcttttg ctttgccgac tacaccatct aactgatcga tttgtttcat
     4081 ttcttcttct gttaattcaa aatcaaataa ttcagtattt tctttgattc tgcttggtgt
     4141 cactgactta ggaagaggaa ggaattcgtg ttgcagcgac caacgcaaag caacttgtgc
     4201 aatcgtcttg tcatgtgctt cagcgatttg tttcatttca ggcacatcga agattttacc
     4261 agtgcctaat ggactatatg cttcgaggat catatcatgt ttttttgcat actcaactac
     4321 agttgtctgt aattcgcctg gtgcaaggaa aacttgattg accatcggca taatttttgc
     4381 tgtttttgct agtgtatcta gatggtgggg taagaaattg ctcacaccga ttgctttgat
     4441 tttacctgct tcatacagtt cttcaaatgc tcgccatgta tctgcatttg cttgttccca
     4501 attatcacga aaagcaacag ggtttggcca gtggatcaaa aacagatcta aataatctgt
     4561 ttggagcttc ttcaatgatt cttcaaaaga agacatcaca agttcatagc tgtggtttgc
     4621 attccataat tttgttgtca agaatatctc ttctctagga attccacttt ccttaatcgc
     4681 ttggccgacg ctttcttcat tcttgtatcc ttgggcagta tcgatatggc gataacctgc
     4741 agccagcgct tctttaacag aggaaacagc gacatcccca tcaggtgttt gccatgtgcc
     4801 gaatcctact accgggattt cgtaaccatt tgatagacga taagtgcttg ttaatgaatt
     4861 catactttca gctccttttt tattcgttca actataagtg tagcgcattt ttcgaaaaaa
     4921 agaatccatt ttgtttctac tcttgaaatt tttgtttaaa agaaaatgat tttgattctg
     4981 gtatttttga ttttttttcc gtaaactaag acagaaggga atgagaaaat ggatatctat
     5041 ttaaaaaaag caattctgca tattattgac agagaaactg gttctccagt cttttcacaa
     5101 aaagagctgg atctgacaaa agaatatatt cgtgattttc ttcaaaagaa gatccaaaaa
     5161 atctcttctg ctcaaacaaa aactggacaa ctagcagaag aaagcatgat cgttgaatca
     5221 cttaaaatcg ctcaagaaaa ttttgtggaa gcaagtgaag caatcgttca gcggtggttc
     5281 gatatctacc aagaaagtga agaagcacct agtgcagatg tatttgttgt cttgtatgaa
     5341 ttagatacag tgatgtactt atctttattg aaagtcaatt atcgtgaggc ttacactcac
     5401 tttgttgaag cagacgaagc agggatcgac aaccgcttga ttttgaatcg agcaattcta
     5461 ggggcgaaaa cacaaaaagc agatgaagca ttcgctgtga atttgactga ttggacctac
     5521 gaattaattg aaaaaaaata tgagttttct ggcaaaaaag agctttattt ttctggaaga
     5581 gtgatcgaaa gtgttcctgc accttcttta gaagaaaatg taaaagtgat ccaaaaagta
     5641 gccaagcatc ttggaaaaaa atttgagaca gaagaatttg atatcatggc tgatttgaaa
     5701 gaagctgtct atgatacgat agaagaaaaa ggtcggttgg atcatgagat gattgcggaa
     5761 aaagtcttca aggaaaacat cacggcaaaa atggctttta aagaagaagt acaagagcaa
     5821 ggatttgtcc cagaagcacc tcctgtaaaa gaagtacaag aaatttctga gaaaaaattt
     5881 ggaaaacaaa aattcaagct atcaaacggg atcgaactga tcgtaccagt agatgtatat
     5941 cgcaacccag atttgattga atttgtgaat aatccggatg gtacgatatc cgtgatgatc
     6001 aaaaatgtgg atgaagtatt gaatcgttta taaaaatgac tagaatatta ggtgttcaag
     6061 taccagtgat cagttaaatg aattgtgtca agagaaggat cggaaaagac gaatataaca
     6121 aaaaaagagc gtgaga
//
LOCUS       DAJSKS010000143         5997 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJSKS010000143 DAJSKS010000000
VERSION     DAJSKS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.147"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(38..1489)
                     /locus_tag="ON396_002426"
     CDS             complement(38..1489)
                     /locus_tag="ON396_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR3114479.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(1810..2574)
                     /locus_tag="ON396_002427"
     CDS             complement(1810..2574)
                     /locus_tag="ON396_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HCR3114480.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(2602..3300)
                     /locus_tag="ON396_002428"
     CDS             complement(2602..3300)
                     /locus_tag="ON396_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HCR3114481.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3312..4091)
                     /locus_tag="ON396_002429"
     CDS             complement(3312..4091)
                     /locus_tag="ON396_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HCR3114482.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4107..5051)
                     /locus_tag="ON396_002430"
     CDS             complement(4107..5051)
                     /locus_tag="ON396_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR3114483.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5097..5876)
                     /locus_tag="ON396_002431"
     CDS             complement(5097..5876)
                     /locus_tag="ON396_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114484.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1637 a         1249 c          999 g         2112 t
ORIGIN      
        1 cactttagta atattttcta tttataacac tttcgtccta cattgatccc cccctctgaa
       61 gtacagtttt tactgtctta aataaaattt taaaatctaa ccccatactc caatttttta
      121 tatattccgt gtcaagtttt gctacttctt caaaatcagt aatatcactt ctaccactta
      181 cctgccacat tccagtaatt cctggcttaa tagcaattct tgcacgatga tgataatcat
      241 attcttcata ctctgattta aggcatggtc gaaatcctat aaggctcatc tttcctgtta
      301 gaacgttgaa aaattgaggg aattcatcta aagacgtgga tctaataaat tgaccgattc
      361 ctgtcttttt tgttccatct ggaagtattt tatttccgat aattcttgga tcaaactcca
      421 tcttaaacat aagattattg ctcatcttat tttctttcat taattctact tttctttctt
      481 ctgcatccat atacatactt ctaaacttat acatcttgaa cggtttacca ttctgaccta
      541 tacgttcttg tgcaaagaaa attgggcctg gtgaattaat ataaataagt ggagcaataa
      601 agataaaaat aattcccgtt gcaatacatc caacaatacc tgcaagaata tcaattgttc
      661 gttttataaa tgcctgttga gttgtcataa cattgatact tgttgtaagt actgtatatt
      721 ctcctatacg accaatagat tgttttccca aagcactatc tgatactttt gcaagattca
      781 aatgtacagt aaggcccatt tcagagcatc gttcaataaa ttctttcata cacgaagtac
      841 ttttattcaa aataataaag acttcatcta cccatccctg acataagtaa tccgctgcat
      901 ttttggaatc agaaacaact gtcactccct caacatcttc accaatcatg cttttatcta
      961 tgataatgac accatttatt ttatacattt tatagttatg ctctttcaca ttacgaataa
     1021 ctacttcagc aatactacta gatgtaacta taataagtga tttgttcccc tcatttttca
     1081 ttttatgttt caagtaatgt ttccataaga ggcgagttat atatgtaatt acaacgtata
     1141 aacctcccat caagaaaaga acgactctag aataatcatt tccaccctga acactaatga
     1201 gatacaaact tcctaacagc accaacataa tcgcttgctt tacagaagaa gcaaattcta
     1261 tatagtaccc tctttttaat acacctctat atgcttcaaa catgaaaata actaaaagat
     1321 ctgccagttc aatgaagact gccatgtttt gatacattgg aatcacataa ggattccaat
     1381 ctacctggcg taagacatac gatagataaa aagcaagctg gatacacaat aaatctaaaa
     1441 taataaaatc aatatgttta acccatcccc ttgaactttt cttgtacatt cctttttact
     1501 cccctctttt taataaaata aattgtatta cttgttttct ctcctaaata tctttctgaa
     1561 ggattattga ctcgacaaat tggcttgtgg atattcttat atatagaaca tccgtaagcc
     1621 aacttaaaac tccttaacaa aattttttta ctcatattgt agatcaaaac aagtatccaa
     1681 gaaatcatat tttatttctt ttttctgctt actcaacaac agctaaaaga aaaatcatca
     1741 taaacaaatt caactaagcc aacgatcccc ttgtgaaatc aagttttttc ttgatttacg
     1801 aataaagagc taaaataaac caaacttctt cttttttacc tcttcataga ctggataact
     1861 gatttcatcc ccgttgatca gatcctttgc gacttgtttc atctgtacta ctttttcctt
     1921 accaaagtcc tttgcaatct gctcatagca ctcttttaag taaaaggtgc gcttagagac
     1981 cccatgggca tcagaagcag ccatctgcac gagattatgt ttgaccatcg ttttggctgt
     2041 tttctggatg tctttgccaa ataccccaac ataacttggc gcagtgagtt gggccaaaca
     2101 tcccatatct aggaacggaa gcaaatgatt tgggtcttcg cgaaaatgca cgttgcgttc
     2161 tggatggacg attactggtg tctttcctag ttctaacaac tcaaaaaaaa gccgttctgc
     2221 atataacggc acttccagtg tcggaaattc gatcagaaga tatgtatcat ctaaatctgt
     2281 aaataatatt tcatctcggt ggatgtcttc gatgagcgtt ccggtaatac gtacttcttg
     2341 tccttccaat aacgtcaaag gcaactgtct tttctctaat tcggcttgta acgaagcgac
     2401 tagagaaatg acttgtgatt tttcattgct gtattttcca ttattgtggt gtggtgtgca
     2461 taaaatatgc gtgatgcctt gctgaatcgc tttttcagcc attgcaatac ttgcttctaa
     2521 gttttctgcc ccgtcatcga ttcctggtag gatatggcaa tgtagatcga tcatttctct
     2581 tgtctcctct cttcttattc cttaatttcc gtagtaataa ccagcatctt tgctatgttc
     2641 tgctccgttg tacacaacac ctaagactcg tgcttgtacc atctccagca gttcttttgc
     2701 tttagtcaag gattctttac gtgacatatt ttcacgtacg accaataaag tgccatccgt
     2761 ttttaaagac atgatctgtg catctgttac ggctactact ggtggcatat cgaatatcac
     2821 cacgtcaaat aactgtcttg cttcggatag gatctgatcc attcttggcg agcttaacaa
     2881 ttctgatggg ttgggtggct taggtccact aggcaaaatc gacaagttct cgactggcgt
     2941 tcgttggatc acatctgcta cacttcctga tgaactaagt gctgtactta atccactggc
     3001 attgttcagc tggaatgtct tatagacgac gggtttacgt aaatctgcat cgactagtag
     3061 aactcgttga cctgatttag caaaaacgac tgcaatgttt gctgcagtgg tggatttccc
     3121 ttctccagga cctgatgaag tcacgacaat cgttttgatt tgctgaccag ctgcggaggc
     3181 aaactggata ttcgtccgga tcgtacgata ttgttcggcg attggtgaag atgggtttgt
     3241 taatgtgatc aaactgactg catgagtttg tgctgctttt tgtttctgtg ttcgtgccat
     3301 ttttcttcct cctagactct tgagcggctt ctgcgtgatg gggctgtttt atcgtttgta
     3361 gcttttggtc gtgcaggagc tgttcctttt ttacggatac cagcgttcaa ttctttcgct
     3421 gacatttctg gaactgttcc taagattggg aatcctaatg tatctgtcac atatttttca
     3481 tctttcactg tccgatcgag taattccaat aagaatgcca gacctacacc taccatcatt
     3541 cctaacatta agccagtcat taaattcaat ttattatttg gcgaaacagg tgtcgtattg
     3601 gcaactgcac ctgaaatgat cgagatttta tcaacattca tcacatcttt tgcgttctct
     3661 tggaaaactt gtgaagttgt gttagcgatc tgttgagcag ccactgcatt cgtacttatt
     3721 gcctgaatcg agaacatttg tgaattttct gcctgattaa ctgagatggc atctttgatt
     3781 tgttcagcag tcattttcac attgtcttct gtttctaaac gatctttgac ttcattgagc
     3841 actaaatcac tgacgatcat atctttatac gtattgatca tttgcaggtt cgtattgaca
     3901 tcattgacat ttgcagtgtc tgattgtggc agtgtcacga tcaattgtgc ttgtgaactg
     3961 tattttggtg tcaagatgaa aaatgttacg attcctgaga tggccaagcc agcaaacatt
     4021 gagatgatga ttattaccaa gtgtttgcgt aatgtttcaa aaatttcttt caagctgatt
     4081 gtctcttcca ttttttctcc actttcctat ttattctcta gattcatttg atcttttaat
     4141 tcttttttca cacgatcaag ttcttgatca ctgacttttt gataggagat attatcttgc
     4201 atgaagcctt ctccttgcag ctgatcttgc ttaatcgtac caaatgcctc ccgataatct
     4261 aaagcaattt ttttcatttc atcaaagttc atgtctgttt tcatattcga ctctagagca
     4321 ttcaaaacgg attgatagtt gctgacaccg tttagactca atactttttt cgctacgcct
     4381 tctacgatct ttctttggcg cccttgtcta ccgtaatcac catttgggtc ttcataacgc
     4441 atacgagaat aagataatgc ctgatccccg ttcaatgtaa ttttaccgat tggaaagtca
     4501 tagccatctt gtgagaaagt gaggtcatta ttgacttcga taccaccaac tgcatcaacg
     4561 agttccttta gtcctttcat attgatagag acatagtgat cgattgggat gtctacatat
     4621 ttttgtacgg tatccatcgc catggcagct ccgccgaatg cgtaagcgtg gttgatcttg
     4681 tcttttgtgt cacgacctac gatgtcgaca tacgtatctc gaggaatgct gacgatcgtt
     4741 gtctgtttgt cttcaggact gactgtggca accatcatcg tatctgatct tccttgatcg
     4801 acccgaccta aatctccagt atcaatcccc atcaatagaa cagagaaaga ttcttgtttg
     4861 tccagatcaa ctggtttcgt tcgttctcct tgggttcgtt ccactgattc gtaagttttc
     4921 tcaatagaat gtgagagatc cagatataat tttgctccta ctcctacaat tgctaatact
     4981 aatacgataa gaactgctaa aataatcaaa aatatttttt tccctcttga ttttcttctt
     5041 cgtcttgcca tagtttcctc cgtttgctaa ccttgcttga atcaacgttt taactgtcaa
     5101 tagcttatat tgctatagcc catttgctga gcaacagttt gtgcttttgt tgcgtatgcg
     5161 gctcttattt tttcattccg gatctgctct atttgactga gcgccgtcaa atattgatcg
     5221 taagtagcgg tagctgttac ttgaccgtct ttcagcatcg tatcgaatag ctgatctagt
     5281 tgcgtgactt gtgcaatttg ggtatcagcg aatcctagat attgcataac gactgttttc
     5341 caagcactat ccttaaaaaa gctcatattt tcacgtacat gggcgacatc tgcacttgcc
     5401 aacttttctt tgatgatgac atcatccaca gcttgctgcc agtttgatac gtttttagta
     5461 aataatttat tgacagccgt ctggatcttc agttttgatt ctgcatcctc taactgcgtt
     5521 aagactgctt tcttttcttc ttggatcgca gcagcttctt taggtaattc tgattccttg
     5581 atgtcataat catctgccga aagtttgata gaagatagtt tatgtgacaa atttgaaact
     5641 gtttcttctg ttatttcagg atttaggaaa attttttctt ttgtactgta cagcttatct
     5701 acttcttttg ataaagaggt cacggtgtct ttttgattgg tgatggcaga ttctgcgtaa
     5761 ttgatccgtt gatgatattg ccgctgatta ttgagatggt atccaaacaa gcaaatcaca
     5821 atagcgcctg tgcctaccgc cacagtagcc aataacttga cgaacggctt ttccatagat
     5881 tcactcctaa atattgatga tagataagta aagatgtgat tcattcacat cagaatgagc
     5941 gctttttatt ttaaaagcat aattaaacta atatattcat agaaatattt ataaaca
//
LOCUS       DAJSKS010000144         5864 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJSKS010000144 DAJSKS010000000
VERSION     DAJSKS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.148"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            199..1095
                     /locus_tag="ON396_002432"
     CDS             199..1095
                     /locus_tag="ON396_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCR3114485.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1245..2186)
                     /locus_tag="ON396_002433"
     CDS             complement(1245..2186)
                     /locus_tag="ON396_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR3114486.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2312..3433
                     /locus_tag="ON396_002434"
     CDS             2312..3433
                     /locus_tag="ON396_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR3114487.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3691..5193
                     /gene="eat(A)"
                     /locus_tag="ON396_002435"
     CDS             3691..5193
                     /gene="eat(A)"
                     /locus_tag="ON396_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCR3114488.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5221..5787
                     /locus_tag="ON396_002436"
     CDS             5221..5787
                     /locus_tag="ON396_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR3114489.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         1925 a         1035 c         1228 g         1676 t
ORIGIN      
        1 ctaatgataa caaattttca gacaattaca actattcaga aaacttttag gcgaatttta
       61 atagaagaat gatttatttc atcatatttt catacaaatg ttaaattttc atacaaatat
      121 aggaaacgtt ttactggctt tttactattt aaacaggcta tcataggaaa aagtgacaga
      181 gaaagtgagg cggaaaagat gaacaaaaat gacgtactgc tagttgcaaa aaataaatca
      241 ctttattttt tgccttccat ccgctcttta gatgtttggt ccggaaaaga tttactacag
      301 tcggaattca ttaaatcagc tagacaacag ttggcaaatt atcagctgat cgttttttta
      361 gattatggat ttgatatgga gatggctcga cgcgtgcgcc catttaccaa tgcaaaaatt
      421 gtgttatttt tttggaatca cttcaaagca gaacacgagg cgatgctgaa gcaggcacaa
      481 gatgaaccgg caatcgatga gatttatcat tttgattttt tggaagcaaa agaacttggg
      541 ctgaaacata atagttcatt ttattcgaaa catatgaatt tttcttctag cgaaccaaag
      601 acggatctat tttttggtgc aacgaataat gggagaaaag aacgagcaga aagctataag
      661 caggaattta taaaaagaga tgttacgacc aactatttca ttcttccaca tcgagggaat
      721 gaacaacctg gatatcttac ttatgaagag tacctgaaaa aaacagggga atcgcgcggg
      781 attctggaac tgttgcggga gggtcagcaa ggagtgactt tgcggacatt tgaatccatg
      841 tattttcaaa agaaactagt gacggataat caagcgattg ctcattatca tttttatcgt
      901 cctgaaaata tttttttgct tcgagaacgt gatttagatg aattaccgga atttctgcat
      961 acaccattta agccaatcct atcagagcta ttggaatttt atgatgcaga aaattgggca
     1021 aaacgattta tcactaacga ttccactatt tatgagcaat acgaatatga cctctcctct
     1081 gttgaaaaca aataaaaaac ctacattttg aagagattat gtactgaaac aaacaagcac
     1141 aattcttatg gcatcatgag agttgtgttt tctttttgat cttttattgg gcttaaaaaa
     1201 agagaaccag ctgctgattc agctgattct catcagagag ccttttattc tgcgccaagg
     1261 attccttctt ttgcatggac taattgggtc aaaaaggcac aatatggtgt agcaatatca
     1321 tatttttgtc cttttcgcca aatggcacca ttgatgtagt cgatttcagt caaacgatgg
     1381 tttttgatca ggtcttggta catagatggg taatgcaacc cgatgttgtc aggatcataa
     1441 gtagaagcaa tatgttggta cacttcttct tgatctagta tgattccttc ttttgctgcg
     1501 atagaagcaa attcagatac gattgttcga accatgctct ctgccgcttt ttgcgcgcct
     1561 agttcagcaa tattacaatc caaaatcgtg cataaaccat tcaatgttcc atttacacaa
     1621 gcttttcgcc aaatggaata acgaacgtta tggctataga tcggatttaa atttgcttct
     1681 tgaaagactt ctacaacttt ttttgtaaat gcttcgcctt ctggttccaa gttctctagt
     1741 tcgatttctc catcacctaa caatgtgact tttccgggac cagctagacc agctgtccac
     1801 atcgtgatcc caaataagat atttttcttt ggcacatatt tttccagtac atcttcatga
     1861 cctaatccat ttagtaaaca caaaacataa gtattgtcag taataatgga ttggatcgaa
     1921 caaaacatat catctaattg attggctttt gttaatgcta cgatcaagtc cacgtgttcg
     1981 ttgctttcga tgatttcttc tggtgaatag attggcagtt ttgcgaccac ttctttaccg
     2041 ttaaagtcag caatcaaccc atttgtacga atggcttcaa tgtgagcagg ccaacgatcg
     2101 atcaagagga cctcatttcc gctttgatgt agcatcaatc cgattcggct gcccattgct
     2161 ccggccccag ctattgcgat tttcatgttt tttctctcct ttattcaatg agttcccttt
     2221 tagtatatag aaaaatggaa gaaaatagca ttgttttgtt tgaaaaaaat gaaaaaaagt
     2281 gtcttactta agaagaaagt gagggataca gatggatatt ttggcggcaa ttgattcatt
     2341 taaaggaagt gcttcttcat ttgaactaaa tcaggcagtt ctaaatggct gggaagatcg
     2401 taaaggaaga aaaatcaatg tgccgattgc agatggcgga gaaaatactg cagaagcaat
     2461 ctattacgcg ctcaggggaa catggcgtac cattgatggg atggatctct tattccgtca
     2521 gcgaaaaatc agctatttat tgacaacttg gcaaggaaaa aacaccgcag tcatcgaatc
     2581 tgcacaagca ttggggttag atctacttga taaaccaacg gatcaaacta tccgacttgc
     2641 ttccagttat gccttaggag aacttattaa agatgcactg cttcaagaag tagaacaaat
     2701 tattgtcact ttaggaggaa gcgggtgtag cgatggtggt ctaggattac tccaatcctt
     2761 gggtgcttct ttagaagggt acacagaagg aaatccttta ttatctacta aaaaacttca
     2821 ttttgatcga agaaacgttc ttgtttctaa agtaaaattg attatcgctg cagatgttac
     2881 caatccatat acaggtcaac aaggagctgc ttggcaattt ggaagacaaa aaggaggaat
     2941 agatacgacg ctagctttct tagataaaca agcaggaaag attgctcagc aaataaaaga
     3001 tttctgggag gtggatttag atgatattcc aggaagtgga gcagcaggcg gtattggtgg
     3061 agcccttttt ttgttgggag cgaagatggt ttctggtttc gatttagtca gccaaatcac
     3121 tggattggaa aaaaagatcg aacaagcaga tgtcatcatt acaggagaag ggcggatcga
     3181 tcagcaaacg atccacggaa aagttccata cggtgtagca gtgcttgcaa aaaaatatcg
     3241 aaaaccaata atcgctattt gcggaagtag agaccaagag ctagaaggac tttctgattt
     3301 tctcccaatt gtcttaagta ttcatttggg tccaataggt ttagaagaag caatggaaca
     3361 caggcaaaca ttagaaaaaa caaaaatctt aggacagaca cttagcagat ttttcaccat
     3421 cctcccgaag tagttgacaa aatcattttc tttatgctat cgtaatttca aatataggac
     3481 aaagtgaggg atccgtgcat tgaaaaatta agtcgattga tgaaagctgc attcagactg
     3541 taccttgtgg agacaaggaa gcagtgtgca gattttcggg attggcttgg ttttttcgat
     3601 gtatgggtct ttttgtgtgt atccaaccct tgcatcctct ttagccagtt ctgaaaatag
     3661 gactggtctt tttttacgag gtgatggaaa atgtctaaaa tcgaaataaa aaatctgaca
     3721 ttcggctacg acagccaagg cacattatta tttgaacaag caaatctaaa ttttgacaca
     3781 caatggaaac taggacttat cggacgaaac ggtcgaggaa agacaacttt actgaatatt
     3841 ctacaaaaca aactacctta tcaagggcaa gtaatccatc agcaagaatt tgcctatttc
     3901 ccgcaacaga caaaagataa agaacgttta acctattacg tgttaaatga tattacggat
     3961 tttgagatat gggaaatcga aagagagctc caattgatgc aaacagatcc tgaaatctta
     4021 tggagagaat tcagcacact atcgggggga gagaagacaa aagtcctact ggcactttta
     4081 tttgtggatg acactcattt cccgttaatc gatgaaccaa cgaatcattt ggatatctct
     4141 ggtagaaaac aagtagcggc ttatttgaaa aagaaaaaac aaggcttcat cgtggtcagc
     4201 catgaccggg gatttatcga tgaagtagtg gaccatgttt tagcaatcga aaaaagtcaa
     4261 ctggaacttt atcaagggaa tttctctatc tatgaagaac agaaaaaact tcgtgatgaa
     4321 tttgaaatgg ctcaaaatga aaaattgaaa aaagaagtca gtaggctaaa gaaaacagca
     4381 gctgaaaaag ccgaatggtc tcgttcccga gaaggagata aaacaaagaa acaagtcgga
     4441 ttcatcgata ctgaatctag acgagtgaat aaaggagcag tgggtgctga tgctgcacgg
     4501 acgatgaaac gatccaaagc aatcgtgaat cggatggaga cccagatcag cgagaaagaa
     4561 aaactattaa aagatatcga atatatcgat tctttgacga tgaatagcca agcgtctcac
     4621 cataagcgac ttttaagcgt agaagatctt caattagggt atgaaaatct gttattcgag
     4681 ccaattcatt ttacaatcga gcctcatcag cgggtggcga tttcaggtcc taacggtgca
     4741 ggaaagtcat ccattatcca ttatcttctg ggggcattca acggcaaggt tataggagaa
     4801 aaataccagc caaaacatct gagcattagt tatgcaagcc aaaattatga agacaatcga
     4861 ggaacgttgg cggaatttgc agagaaaaac caagtagact accaagcatt tttgaacaac
     4921 ctccgaaagc ttgggatgga aagagatgtt tttcataaca agatcgagca gatgagtatg
     4981 ggccaacgga aaaaagtgga attggctaaa tctttatcac agccagctga actatataca
     5041 tgggatgaac cattgaatta tttggatgtc ttcaatcaag aacaattaga acaactgatc
     5101 ttgaacgtga aacctgccat gttactagtg gaacatgatc aaaccttcct ggataaagta
     5161 tctactgaga ttatttctct tgagagaatc taacccttgt aatctcaaga acaacaaggt
     5221 atggttaagg ggaactttgc caacgaagga gagaatcaca tgctttatgt aacgagacac
     5281 ggagaaacaa cttggaacgc acaaggatta gtttgcggac gggcagatgt gccgctgact
     5341 gaaaaaggac agatgcaggc acagaaacta gcagaaaagg tagtagattt gcctgttcca
     5401 atcacgaaaa tcatccattc ccctcttcaa cgagcaagag atacagcaca agcagttgct
     5461 gatcgtctca gcttgccgct gaccgttgat gaacgattgg tagaaatgga ttttggggat
     5521 tatgatggac tgccaagcaa agatgagaac tttcaaaaag cacgtttagc gttcgctgta
     5581 cgattcccaa atggggagtc agtcttagat gtgtatgcac ggatcgttcc tttattgaaa
     5641 gaatgcatcg aagacgaaga aaatgtctat ctgttagttt gccataatgc cttgataaga
     5701 gtgatcaatg cttattttca tccgatgcct aatgaggggt tctttacatt catggtagat
     5761 aatacagaat tggtttctta tgagtaatta tgtttttgtg ttatttaaat aaaatatttc
     5821 aaggcactgc ttttgcttag cagtgccttt tttaggttat gaca
//
LOCUS       DAJSKS010000145         5844 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJSKS010000145 DAJSKS010000000
VERSION     DAJSKS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.149"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(70..528)
                     /locus_tag="ON396_002437"
     CDS             complement(70..528)
                     /locus_tag="ON396_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR3114490.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(541..1872)
                     /locus_tag="ON396_002438"
     CDS             complement(541..1872)
                     /locus_tag="ON396_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HCR3114491.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1883..3325)
                     /locus_tag="ON396_002439"
     CDS             complement(1883..3325)
                     /locus_tag="ON396_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3114492.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3312..4076)
                     /locus_tag="ON396_002440"
     CDS             complement(3312..4076)
                     /locus_tag="ON396_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HCR3114493.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4180..5055)
                     /locus_tag="ON396_002441"
     CDS             complement(4180..5055)
                     /locus_tag="ON396_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3114494.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5310..5394)
                     /locus_tag="ON396_002442"
     tRNA            complement(5310..5394)
                     /locus_tag="ON396_002442"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5358..5360),aa:Leu,seq:caa)
     gene            complement(5412..5483)
                     /locus_tag="ON396_002443"
     tRNA            complement(5412..5483)
                     /locus_tag="ON396_002443"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5449..5451),aa:Gln,seq:ttg)
     gene            complement(5493..5565)
                     /locus_tag="ON396_002444"
     tRNA            complement(5493..5565)
                     /locus_tag="ON396_002444"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5530..5532),aa:His,seq:gtg)
     gene            complement(5627..5711)
                     /locus_tag="ON396_002445"
     tRNA            complement(5627..5711)
                     /locus_tag="ON396_002445"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5675..5677),aa:Leu,seq:caa)
     gene            complement(5732..5802)
                     /locus_tag="ON396_002446"
     tRNA            complement(5732..5802)
                     /locus_tag="ON396_002446"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5768..5770),aa:Cys,seq:gca)
BASE COUNT         1777 a         1249 c          891 g         1927 t
ORIGIN      
        1 atatcatttc aaaaaaattt tgtaaattca cccataaata tcaccctatt taactttttt
       61 catcgtacac taaaattcaa aacaactatc tttaatcttt acttcttggt tttgttttaa
      121 taaatttcct tcaaaaatgc ctggagaaac tacaatgatc accgtatctt tttgtgcttg
      181 ctccaacttc ttcaaatcta taatagctaa cagtgtccct tcttttacct cctggttact
      241 ttcaacccgt atctgaaacg gacttccttc gagttcaact gtatctaaac ccatatggat
      301 cagtattttt ctgccagatt cactttcgat cgttatggca tgtcgggtag gaaatatatt
      361 atcgattatt cctttaactg gcgcatggat atatccatca tgattcgtta tagcgtatcc
      421 aactcctatc atttttgaag aaaagacctc gtcttttact gctgttaatg gaataatttg
      481 accactagct acagctttta gaatgtttct tgttttaaag aaatgcactt tttatcacct
      541 ctactttaat gctggccaat attttttgtt tactttgatc atttcatcta gtattttttt
      601 tgcgactctg gcatttggta cggtttgatt caatgtaaat gcttgtaaag ctttttgata
      661 agaaccttca aagtaagcat ctactagtaa cttctctact gcaacttgtg cctccatcaa
      721 tcctttatga aaatcactga ttggttttct tagtgtaatt ggctccacac cagttgcacc
      781 aacatatgcc ggtatttcaa caactgcatc tgaccgtaga ttcggaatcg ccccttgatt
      841 aggaacgatt agcataaagc gacgacgatc atcattcaac aaggaaatag ctatatcgac
      901 gatatattgt ccatgctccc caaaattaaa gttcaatact tcttccacaa taccgcggcg
      961 tattttatca gccatttctt tggtattttt ctctcgtcca tccatgacca tattagcacg
     1021 tgtatactct ttgtcagtat gctcaacgac catatcagga tataagtaat actctaagta
     1081 attattcggg atattacttg gaaaattttt cgtgatgact gacatcattt gaaaggtttt
     1141 ttgccaagtt ttatctccta tattaaaatc ggcgacttgc atttcttttg ttattagttt
     1201 ttcaataatc tctggcataa tttctcgttg caattcttta tcataaatcg atgtatacca
     1261 gccaaagtgg tttaaaccat aatacgtaac gatccaattc ttacgatcat aaccgtaatt
     1321 cactgcaatc gtttcttcaa ttgaaatagt catatcacag gcattaatca tttttacatt
     1381 cggatattta cgacgaacag cttcagaaac gatcgactct ggatttgtat aattcaaaat
     1441 ccatgctttg ggggcatagt cttgtatatg atcgacaatt tccaataaac ctttgattga
     1501 gcgcatccca taagcaaatc ctccgagtcc acacgtttct tgtcctacta atccatgttt
     1561 aagtggaatc ttttcgtcct tttctcgcat ctccaatcct ccaacacgaa tttgtgagaa
     1621 aacaaaatcg catcctgtaa aagcaagctt tggatcatcc gttgcacgaa tgcgaatatt
     1681 tgaaaaacct tttcttttca gcataaattt aacaatcaag tacatatcca tatttctttc
     1741 ttcatcaata tcatataaac gtatttcaga taatggcaac cgtttttcat tgttcaaaat
     1801 ggcttgaacg attcctggtg tatacgtact cccgcctcca gctatagtga taatctgttt
     1861 tttcatcatc atcttttcct ttctattctt ctaataattt attgaattct gccataacat
     1921 aagtcacttc taagccgatg ataatttgat aatttttttc agttggttta atgatcccgc
     1981 ttatccctaa ttgcttgatt cgctgttcct cgaccatggt gggatccact acctctaccc
     2041 gtaggcgtgt attacaacta tagacatcca ggatattggc ttttcctcca aacgcttgta
     2101 gcatttttgt tgccatatat tcataatttt tacttgctaa ccctaactct gtttcttcta
     2161 aggttacttc attgccaaac tctgccttag cttcacgtcc taatgtctga atgtttctct
     2221 tattgatcaa ataataaaaa gtaacgtaat acaaggcaaa aaagaaaatt ccgattggaa
     2281 gaatcaaaat agcattggta gcgattcgaa aattgatgag ataatcaaca atacatgctc
     2341 cgaatgtaaa accaattcgt atattgaata agtaacagac aatccctgct aaaccggcaa
     2401 aaagtgcatg gatcacgtac aacataggag aaagaaacat gaaactaaat tcaacaggct
     2461 cagtgatatt tgcaataaat gaagttcctg caccgcttcc catgattcct ttgaccatct
     2521 ctttattttc tttataagct gctttataga tagctagaca tatagctggt ataccaaaca
     2581 tcattactac aaaaaaaact gataaaaaca gtccagcctg aggatctccg ttgataaagc
     2641 gtgtcatctc tcctctgaaa acttccttgc taggagatgt atagcttcct aaatcgtaat
     2701 aaatatacgt attcaacaca tggtgcagtc caaaggggat cagcaaacga ttcaaaaaca
     2761 taaatatccc cacacctaat gctcccatat caacgagcag ttttgcaaaa ttatcaatcc
     2821 caatttggat gagcggccag ataaagccca aaaaaacagc tgcgattgtt tgtagaatca
     2881 tcaccatagt caatgggaat ttttcgcccg caaaataaga aaaaactact ggtaattttt
     2941 gttctttaaa gcgattatac gtccatgccg ctactaaacc ggaaataatt ccaccaaaaa
     3001 tccccatatt cacttctggg tccataattt ctaagccttt tttcaaggta gcaatcgcta
     3061 aaaaaccagt aagtgcaggt attcctttgt ccttattttt gacaaaaccg attgcaactc
     3121 ctatagcaaa taagatatcc aagtttccaa aaacggcatt tccagctgca ccaattactg
     3181 ggatgttcag catatcgtca gctgataaac gcatgatcaa tcccgctaat ggcatcgctg
     3241 caactggaag tagcatcgct cttccaagct tctgcacatt tgatttaaca ttcattttta
     3301 ttcctcctct attaaatatc ctcatatgtt actaactcaa tcaaaggatc atttttcact
     3361 ttttgagcaa atgctgaatg aattaaaaat tcgacttggt gggtacgagg atacacaaaa
     3421 cttgaaccat ttaacaattc ctgatccaaa tacgctgcgt gacacatgac ttcgatcgtt
     3481 ttatcttctc tcaatatcat gagtaaactt gccaaatatt cttccacact cgtattgatt
     3541 tgtcgttttt tttcatcagt ttctcctacg acatcgaaac aaggctcaaa ataatcgaca
     3601 tgacgaactt catcttttcg ttcaaagttg ctccgaaccg gcaaatcata tttttttgct
     3661 aatgtaacaa ctacctcctg attttcatcc aaagtatgag tatgatgatg actgtctaaa
     3721 tgagttggta tgatccccgc atgataaatt ttttgaatct gagcattcca ttcctgatac
     3781 agttgatctt tgtcaatgtt aaatggatgc tgataataag ataaggatct gaactctccg
     3841 ctttcgataa gagtatcgac agttttgagg agtggtttgc cacacgtcaa tgttaaatgg
     3901 actcctacac ctagcctagg catttccttt cttagtttca cagcatgctc gaaacccggc
     3961 atatttgcca tcaatgtagt tgatgtcaaa attcctcgct gataagcatc cataatccca
     4021 taattcacac catggctata gccaaaatca tccgaattaa aaatcacttt tcccatttca
     4081 ttctccttct tttttctact aaaataggcg caaccaaata atcatagtcc atttactatg
     4141 tgtcatttgg ttgcgcctat agatagttac gcccttattt taatagattc ttgctagatg
     4201 aattgccaaa tagatgattt cattttcttg aatggcaagt tgaaattgct gttcgatgat
     4261 atctttaatc tttagtgctg tacaatacgc tttgctatgt ttaaatgttt cagtaaaaaa
     4321 accaatattt tctactggcg tttcttcctg ataaatcaag cgtttaacca ggaatttgat
     4381 atgaaccatt aagcgatgat aagtcagtga ttctttatca taaacgtttg aataggattt
     4441 ttctagatgg gacaaaatcg tggtcgtcat ttctactatt tttaagctct ttaaactatt
     4501 aggctctcct agctcagcat taatgatatg catagtgatg aatcctactt catttgaccc
     4561 taactcggtt tgatatcgat gattgattcg ctcgaccact tctactgcta tatcatattc
     4621 atttggataa aggaatctca tttctggcaa cataattgct gtgacatctc tatcttctct
     4681 ttttcgttga atagcaaaat aaatatgatc tgtcaatgta atatagatat ttgaatctaa
     4741 tggagaggag atcctagctg ttatcagttc aatcacttct tccgcaaaat ccataaattc
     4801 gattggaatc tgcgagacta tctcactgaa tcgtttgatc ttttctttag aatctaatac
     4861 aaataatttg tcagcatcct caacatcaaa tggcttactc actttagcag aaaagccaat
     4921 ccctttcccc atcgcaattt tttctattcc atcgtcatcg atcaatacag cattatgatt
     4981 cagctttttc atcacataca ccgttaattc acctcctcta tatgataaaa caaaacaact
     5041 tgcagactcc ttcatacata tttttgtctc caaacaattt ttaaagacaa aaaacgacct
     5101 aagaaagaac cctttcttag gtcgtgccta attgaatagt agcaccctac ataatttaca
     5161 aaaatagttt aacacataat aaaagcgtta tcaatatctt ttttactcgt tttatgataa
     5221 tacttgtatc gaattgattt ggaggtaatt acataaaaac aaaaaaaata tagcaactct
     5281 ctataagaaa gttgctatat tgataattat accggcggcc ggggtcgaac cggcacgtcc
     5341 tcaacggaca ctggattttg agtccagcgc gtctgccaat tccgccacgc cggcaaattt
     5401 aaaaataata actggggtag ctggattcga accaacgcat gagggagtca aagtcccttg
     5461 ccttaccgct tggctatacc ccaagaataa aaggcgaatg atgggaatcg aacccacgaa
     5521 tgccggagcc acaatccggt gcgttaacca cttcgccaca ttcgccatca taaaagatgt
     5581 tatttaattt ctcaataaaa aactaagatg aagacatctt agtcaatacc ggcggccggg
     5641 gtcgaaccgg cacgccctca acgggcactg gattttgagt ccagcgcgtc tgccaattcc
     5701 gccacgccgg caagttgatt tattagtact aaggcggtaa ccggatttga accggtgatg
     5761 aaggttttgc agacctctgc cttaccactt ggctataccg ccattatact attatcaatc
     5821 tataaaataa agaataaatt atag
//
LOCUS       DAJSKS010000146         5696 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJSKS010000146 DAJSKS010000000
VERSION     DAJSKS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.150"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(249..926)
                     /gene="nth"
                     /locus_tag="ON396_002447"
     CDS             complement(249..926)
                     /gene="nth"
                     /locus_tag="ON396_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR3114495.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(933..1631)
                     /locus_tag="ON396_002448"
     CDS             complement(933..1631)
                     /locus_tag="ON396_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCR3114496.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1899..3083
                     /locus_tag="ON396_002449"
     CDS             1899..3083
                     /locus_tag="ON396_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HCR3114497.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3089..3532)
                     /locus_tag="ON396_002450"
     CDS             complement(3089..3532)
                     /locus_tag="ON396_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3114498.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3758..4045)
                     /locus_tag="ON396_002451"
     CDS             complement(3758..4045)
                     /locus_tag="ON396_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114499.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4216..5568)
                     /locus_tag="ON396_002452"
     CDS             complement(4216..5568)
                     /locus_tag="ON396_002452"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HCR3114500.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
BASE COUNT         1501 a         1136 c          965 g         2094 t
ORIGIN      
        1 tatttatata atcctttgga atactggact tattttatag gtatattgga aggcattatt
       61 ttctacatcg ggtattttcc caataaaaat aacaactttt atttttatcg caataaattg
      121 taataagtta ccatcctagc tactgagtat gtacatttac tttttcatgc aaaaaagata
      181 aacgaaacag cttttctcca taataacaag ataattcata catttatttg caattaaaga
      241 aaccacgtct acaggcgtgg tttctttttt agcgcctttt cttttagacg catccgattt
      301 ttcccatctt gacatattga tagcaaagga catacctcgc acttgggatt tcttgccgtg
      361 caatgatatc taccaaaaaa aatcagtgta tgatgtgtct ttacccataa ttcttgtggt
      421 actttccgca tcagtgtttc ttctacttcc atgaccgtag catctagttt gcagatcctc
      481 aggcgttttg agacacgttc cacgtgggta tctactgcga ttgctggtat cccgaatgca
      541 tcccccaata cgacatttgc tgtctttctg cctacaccag gaagagacat caattcttct
      601 cttgacgttg gcacttgccc atcgaaacgt tcaatcagct gctgtgcaca cgctttgatg
      661 ttttttgctt tatttcgata caaaccaatc gtttttattt taagaatgat ttcatcgatt
      721 gaagcttctg ctaacgcatc gggtgtcgga aaagaggcaa acaaatctgg cgttgcttta
      781 ttcactgata catctgtcgc ctgggcactt aagatcactg caatcaataa ctcaaatgga
      841 ttattgtgct tgagttctcc atgtgcttcg ggaaacattc catacatcgt ttctagagct
      901 tccattgttt tttgtttgct taacatattc cgctactcct ccaaccaatt tttcagtgag
      961 actttcggca attcttcttg ttgagctgac tgttctactt ctctttgaag aagctgctgt
     1021 tttcttcgtt tttggtcttc ttccacttgt tgttttgtac gtagattttt tctttcccac
     1081 gatagcaaaa tgcggtcaac gtaattaaaa ctataagcct gatttaatac ggcttcccgc
     1141 agtgctagct tcaaaatttc cggctgataa tggtcttctt ctagccattg tccgatccgc
     1201 tggaactcga tcgaagaaag cgggcgtcca aattcttgtt caaataattg atacactgac
     1261 tggatttctt tttcttgtgt tttttcttcg tatttttttt cttgtgtttt taaatactca
     1321 cctaatagat catagatggg gtaaagatta tatcgatctg cttttttacc gttgacaatc
     1381 gtcgtttcta tcttgataaa tccatttgtt acgagacgat ccagtatttg aaaaatctga
     1441 tcttgtttga ttcccatatc catagcaatc atctgcaaat cagggaaagt atctccttca
     1501 agctgtgcca tatgcaactg tagaacaaat aaaaattctt cagattgtag accgatccta
     1561 tgataatttt taagtaaaag attagatatg actgtttgtc cagcgcttaa gtaattttct
     1621 aaattcaaca tgatttctcc tccgctcaat cacagtatac gtaaaaaaaa catgggaagc
     1681 aagaacaggt ttcttttcta tttacttcac tgatcggtca gatttacaat gtcgtctact
     1741 tgatttttgt ttgttcatgt ttttgatgcg tatcttcact tttcggaata aataaattaa
     1801 gtagatttcg ctgttcctta gaatgttgac aaggaaacaa aattactata cactattttt
     1861 gtttccaagt taacattatt gcataaagga gtttttacat gtacagtttc acaaataaac
     1921 attctaaagt ttttggatac gtctctgtct tattcactgg attaggattt actatcgtca
     1981 gtcctgtact tccgttttta acgctgcctt attctcactc catacatcag caagctttct
     2041 atatcacttt gctcatgtct gtttatgctc tagctgcctt cttatctgcc cccattctcg
     2101 gtagcttaag tgatcatttt gggcgtcatc cgatccttat tatcagttta ttaggatcaa
     2161 gtatcggata tttgcttttt ggtttaggaa attcgatatg gatgttgttc cttggtcgta
     2221 tcattgaagg attgacggca ggagaaatct ccaccttata tgcttacttt gccgacatca
     2281 cggaacctaa tgaaagaacc aaagtttttg gatggatggg tgctttagtc ggtataggaa
     2341 caacgctggg tcctattata gggggtctat tagctgagtt ggggaattct gttcctatat
     2401 ttattggagc actctttact ttcttaaatg ccgtttatgg ctacacattt atgctagaaa
     2461 gccttccaat aaaaaaacgt tcagtagatt taagtttctc ccatgtaaga ccttttcacc
     2521 aattacaaca attattcaag atttcatccg ttattccttt attgacagca ggttttgctg
     2581 tttggttggc agctggctct cttcaatcta tcttttctca attttcgatt gacgcctttc
     2641 agtggaaggc tgggttggtt ggtttaagct tctctttgat tggaatcttg gatactgtct
     2701 cgcaattgtt cattatgcct cgattgctca aaaaattttc agaacagcag atcacaagaa
     2761 tagggatgtt cagtgaaatc ctcgcctatc tattcatcac attatctgga attcttctac
     2821 tgccgatttt gtttttattt gggattatct gttatggttt cggtgattct atttttactc
     2881 ctgtattcaa tggccaacta tccaattctg tctcagataa ccagcaaggt cttgtaatgg
     2941 gcggtaccca aagtatacaa tctctatcgc gcgtcatcgg cccgctaatc gcaggacagc
     3001 tttatgctgc agcaccattt cttccgacgg tattcggatt tttgcttatg atttttgcct
     3061 attatcacta caataaaaaa tagtagcttc atttgctact tcgtataaat tctctgtatc
     3121 gaccgataaa acgaaatact gtgtcaaatt cttcttttga catattttca aatacagtag
     3181 catctcgctg tacccatttt tgatggagtt gctcgtgtct attccttatt ttttgtccga
     3241 ttggcgtcaa ttgaaaatag atttcttttt tattctcttc gctttgataa gtgtttatga
     3301 tccctctagc tatcagtttt tttgttagct tactgatggc tcctctagtc atgtaactag
     3361 cattcgctaa ttttgtcacg ttaggatcag ggattttccc gatatgttca atcacttcta
     3421 gttcatttag agtaaatcct gcaaattctt tttccatttc tggtttatta cgatgaataa
     3481 tctcattagc taaatccaga tattcttcta ttagcagttg tcttttatcc atttctacac
     3541 cttctttttc tttcattata ttgttttttt gtttcctatt caacaaagat aaaaatattt
     3601 tgtaggaaaa cccaatctga aaccaagaaa aaaaggggtt gacaaagctt gtcataaccc
     3661 ctttttctga ttaaaagata gtaaagagac agtcatcggt aattgtgatg attttttgtc
     3721 atcacttaag gatgagcggc cttttcaaaa actgtcttta aagatcatca tcagtttcta
     3781 gcacatatgg ctgaccttca tatgtgtact ctgtattcaa tgtgccgtca ttttcatctg
     3841 agtagatgcg tctactgaaa ggaaaacgat gagaaacatt atctttggcg tcatttgctg
     3901 gcatcgtgta atattcctct tgcgtttcat tcaactgaga gagtacttga tctgccaact
     3961 catcaatatt gatcgttaat ttcttcgaag cacttttata atcttctaaa aatgcatctt
     4021 gttggacaaa atattcttta ttcatcttct ttctcacctt tcttttcaaa atcaaataaa
     4081 gctgcttgtt cttttatgta acagctttat ctttgctgct tattgtgaca aaaaattcga
     4141 agaaagacaa ctaattgtac ttttcacaaa aaaagacaga gggtcgtgac aaaattgtgt
     4201 cacaaccctt tttctttatt gtgcagtttc gatttcgcct agcgctgctt gtgcaagtag
     4261 gttcaagtaa ttccatgggc ggtcaaaatg tggttggaag aagaaatctg aaagtgcgag
     4321 atcttctaca gtcattttat tttgaacagc taaagacatg gtatttgctg attgtgtaat
     4381 gtcgtatttg gacatgaact gagcacctac gatacgattc gttccttttt catagactag
     4441 ctccatcatt actttttcag tagttggcat gaactctgga cgataattat cttcaaatac
     4501 agtcgcttgg acttctagat cgttcaaagt tgcgctttct ttggtcacac ctgtcgaacc
     4561 gatcgtccag ccaaataggt aaagtcctga agttccttga gtaccacgat acgccatttt
     4621 atgatctgtc aagttatttc ccacaagcaa accttgacga acggcatttg ttgccaaagg
     4681 aatatagttt tgcgtgccac ttggattgta gttgactact gcagagtctc cagctgcaaa
     4741 aatgtctgga ttacttgtct gcatgtattc attcactttg attgccccgt ttggcagtgt
     4801 atctactttt ccttcgatca gttttgtatt tggacggaag cccacacata aaatgaccat
     4861 atctgtatca aatgtatctg ttggtgtagc tacttgtttc acttttcctt cttcatctgt
     4921 gataaattca gtaacatttt cgcctagcgc aagagtaaca ccgcggtctg tcaattcttt
     4981 ttctaagata tctgtgaatg gtttatctag atatttattc aagatgcgat ctaatccatc
     5041 aatcaatgtc acttgttttc cagattcgac aaaagcttct actaattcaa taccgatata
     5101 tccaccacca acaattacta cacgttttgc ttctttcgct tgtgcaataa tttcatttgc
     5161 ttgattgtag tttttgcata ggagtacatt tttcgcatcg attccactaa tcggtgggat
     5221 aataggccat gaaccagttg tcatcactaa tttatcataa gaaaccgttt ctgtttctcc
     5281 tgttttcaga tcttttgccg ttactgtttt attttctgta tcgacgtctg ttacatcgtg
     5341 ttccatttta actgttgctc ctaatgaaga taattcttca ggatttgagt agaacaagcc
     5401 tgctgggtcc tttactactc cgccgacata aagtgcgata ccgcaagata agaaagaaat
     5461 attgtcattt ctttcatata ctgtcacttc tgcttctgga tgatttttta aaatactttt
     5521 tacagctgca gtacctgcat gagtacagcc aattactact actttcattt atggtccctc
     5581 cgattaacat tttgtccttc gacaaaacta ctataacaga aataaaacgg atacaacagt
     5641 ttaatgcttg tgaattcaca aaggtttgtt tgtagtttca ttccttttca agcata
//
LOCUS       DAJSKS010000147         5692 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJSKS010000147 DAJSKS010000000
VERSION     DAJSKS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.151"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            314..1444
                     /locus_tag="ON396_002453"
     CDS             314..1444
                     /locus_tag="ON396_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR3114501.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            1536..2291
                     /locus_tag="ON396_002454"
     CDS             1536..2291
                     /locus_tag="ON396_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR3114502.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2349..4388
                     /locus_tag="ON396_002455"
     CDS             2349..4388
                     /locus_tag="ON396_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HCR3114503.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4497..5648)
                     /locus_tag="ON396_002456"
     CDS             complement(4497..5648)
                     /locus_tag="ON396_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HCR3114504.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1933 a         1028 c         1148 g         1583 t
ORIGIN      
        1 taaccttgtt aggtgaggct cctatacaaa cataggctgt tgcccagaaa cgtcgaaagt
       61 cgccaatggg taaaacagaa actgttggat taaggctttt tccaatagct aagaatgttt
      121 tctttacgtt gtatagtgct aaaactcgga cgatgagatg atgctaggtc agaattcttg
      181 ttcgaaaagg tttctgacct tttctttata tttttgtgac tgacttattt gatgagacaa
      241 actcgctcct cacctgacaa gtggattgct gtgtgaggag gagatgcggt gaaaaaagat
      301 cgattggtgg attttgggtc cttatttgac tttgtccatg atcggtcttt tagaagtata
      361 cagtgccagt tcttatcgtt tattgcaagc cgatgaaaat acaaaaagtc tattattgcg
      421 tcaactaatt ttcatatttt tgagttggag cgtgatcttc ttagctcgtt cagtcaaact
      481 acactattta cttcacccta aaatagcagg atacggttta gccttatcga ttttcttttt
      541 agtattagta agaataggga tattcggtgt cactgtcaac ggcgcacaac gttggatctc
      601 tctgtttggc attcaattcc agccttctga actggcaaat ctttttttga ttttttattt
      661 aagctggttt tttcgtgacg gaaatagtag cccaaaagat ctaaaaaaac cattcctgat
      721 tacagtaggt ataacttttc tgattttatt tcagccaaag attgctggag cattgatgat
      781 cctttcgatt gcgtgggtca tattttgggc agcggcggtt ccatttaaaa aagggatcta
      841 tctaatcgtt actttttctg cattgctgat tggagcagca ggcggggtat tatatttagg
      901 aaataaaggt tggcttccac agatgtttaa tcatgcttat gaaaggatcg cgactttaag
      961 agattctttt atcgatagtc atggtgcggg ctatcaaatg acccattcgt tttatgcact
     1021 atataatggt ggcatatttg ggcggggatt aggaaatagt atcacaaaaa aagggtatct
     1081 tccagagaca gaaacagatt ttattttttc gattattaca gaagagcttg ggctaatcgg
     1141 tgcattgtgt gtgcttttct tattgttctc attatgcatg agaatctttt gtttaagcag
     1201 tcgctgtaaa aatcagcaag ctggattgtt tcttttagga ttcggaactt tgctattcgt
     1261 tcagacgata atgaacgtgg gaagtatcgc ggggctgatg ccaatgactg gtgtgccatt
     1321 gccatttgtt agctacggag gaacaagtta tctgatcctt tctttaggaa tcggaatcac
     1381 gttgaatatt tcttcaaaga tacaggccga agagttggcg ctctatcgac ctgaaaaaca
     1441 ataaagagaa actgagaaac tttcagtttg aattatcata aagtacaatc ttttattttt
     1501 caagcgagag gttcttttag aaagaggaat tcgcaatgaa agtatctcaa aaaatcatat
     1561 tatctatttt gttactgctt acattagtag ttggctattt ttcgattgag tttatccatg
     1621 gattttcgtc tgcaaaacag agttcaactg tgaaaaaggt agagccgaaa agtatcccta
     1681 ccacactaaa tgtggctttg attggttcag atgctcgttc gaaagacgaa aacggtcgct
     1741 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
     1801 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
     1861 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
     1921 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
     1981 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
     2041 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
     2101 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
     2161 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
     2221 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
     2281 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
     2341 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
     2401 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
     2461 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
     2521 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
     2581 tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag
     2641 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
     2701 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
     2761 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
     2821 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
     2881 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
     2941 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
     3001 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
     3061 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
     3121 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
     3181 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
     3241 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga
     3301 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
     3361 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaaca gcttttgaca
     3421 gtctaggagg aaaagctgga tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
     3481 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
     3541 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
     3601 atgctcctgg atctacgttt aaaatgatta cagcagcaat cggtctcgac aacggcacta
     3661 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
     3721 gatcttatca agcaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
     3781 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
     3841 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
     3901 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
     3961 gatatggaca gggcgaactt ctaattactc ctatccagca agcagcaatg tattctgttt
     4021 ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag acaaaagata
     4081 agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg
     4141 tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga
     4201 aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct
     4261 tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag
     4321 aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt
     4381 atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc
     4441 ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactcac
     4501 acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag tgtacttaat
     4561 aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact ttgctgtcca
     4621 atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc gaccatcaat
     4681 gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag caatttggtc
     4741 aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa gtcagaaaaa
     4801 tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata tcctaaagcc
     4861 tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat cccagcaaaa
     4921 aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat tcctaaacaa
     4981 ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt
     5041 aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat tagctgttca
     5101 atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa agataggctg
     5161 aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa aattgttgat
     5221 ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa
     5281 gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg
     5341 cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca
     5401 ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat
     5461 aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat
     5521 ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat
     5581 ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata
     5641 aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat aa
//
LOCUS       DAJSKS010000148         5689 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJSKS010000148 DAJSKS010000000
VERSION     DAJSKS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.152"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(488..1297)
                     /locus_tag="ON396_002457"
     CDS             complement(488..1297)
                     /locus_tag="ON396_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3114505.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ON396_002458"
     CDS             complement(1297..2043)
                     /locus_tag="ON396_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3114506.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ON396_002459"
     CDS             complement(2061..2528)
                     /locus_tag="ON396_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3114507.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ON396_002460"
     CDS             complement(2528..2938)
                     /locus_tag="ON396_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR3114508.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ON396_002461"
     CDS             complement(2949..3962)
                     /locus_tag="ON396_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3114509.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ON396_002462"
     CDS             4179..4913
                     /locus_tag="ON396_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3114510.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="ON396_002463"
     CDS             4897..5607
                     /locus_tag="ON396_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3114511.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAJSKS010000149         5626 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJSKS010000149 DAJSKS010000000
VERSION     DAJSKS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.154"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(112..1050)
                     /locus_tag="ON396_002464"
     CDS             complement(112..1050)
                     /locus_tag="ON396_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR3114512.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            1541..2758
                     /locus_tag="ON396_002465"
                     /pseudo
     CDS             1541..2758
                     /locus_tag="ON396_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            2892..3131
                     /locus_tag="ON396_002466"
     CDS             2892..3131
                     /locus_tag="ON396_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HCR3114513.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
     gene            3180..3248
                     /locus_tag="ON396_002467"
     CDS             3180..3248
                     /locus_tag="ON396_002467"
                     /inference="COORDINATES: protein motif:HMM:NF018061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HCR3114514.1"
                     /translation="MLVFQMRNVDKTSTVLKQKSSP"
     gene            complement(3286..4458)
                     /locus_tag="ON396_002468"
     CDS             complement(3286..4458)
                     /locus_tag="ON396_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HCR3114515.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
     gene            4497..4595
                     /locus_tag="ON396_002469"
     CDS             4497..4595
                     /locus_tag="ON396_002469"
                     /inference="COORDINATES: protein motif:HMM:NF018061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HCR3114516.1"
                     /translation="MYRKRAVTWPFIFYTIIWTLSKQTKNSDYADK"
     gene            4720..5457
                     /gene="erm(B)"
                     /locus_tag="ON396_002470"
     CDS             4720..5457
                     /gene="erm(B)"
                     /locus_tag="ON396_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCR3114517.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT         1927 a          978 c          962 g         1759 t
ORIGIN      
        1 ttttaaattt ttatatttag gaggaataat cctctgattt tttcatacgt tatgtcacct
       61 cgtaaatatt aattatactg aattagcaat ttttatcaaa taaaacttat tttacttcca
      121 aaacctaaat tcacgttgcc aaaaatcaat ctgcttttgc aattgttttt cgttcgcttt
      181 taaagtcgat ttcattaatt ccgttaaatc aattggagat atttctctaa tcaatttttt
      241 aaatttagtc ttagtattct tacttagctt tccccacata ctttcttcat gcaacaaagt
      301 ataaaccata gcttgctcat taattttttc taaagtagcc cacgcaggtt tcaagatgtg
      361 taaatcatta aaacaatcat tccagtaatc aaccatatct ctttttaatt caacttctac
      421 acgccataaa tgttcagaca caacttcaac atctgcgtta tctttacgtt cttgtttttt
      481 attataaatt ctaataaatc tatcactatc acggacacca aaatattttg tttctggctt
      541 gccattacga ccataaaaaa cagttttctt aactgcttta tcagtcattg catagtaatc
      601 gctcaaatca tcttcaaaat caaaagctaa gtctaatctt gtaaaaccgt catcttccat
      661 gtagtcgata atattttgtt ttaaccaaat catttcttca tgtgtgagtt tattgggatt
      721 aaattcaaca cgcatattac gtctatccca agtatctgct tttactttgt catattcgat
      781 ataaactttt tcttgaagtg ccttagcttt aaactttgtt tgaagtatat cccaaagtcg
      841 tatttgtggc tctacactca taaagtcaga tagcttttta gcattagttt tgttcaaatt
      901 tccaacgatt gtcatggcat caaaacttaa tgcgggttga gattttccca aagtttgacc
      961 acttaaccgg ctattactta accggctatt agagacggcc ggttttttta gtgagatttc
     1021 tgcagctaat ttttcatcaa ttttcttcat tttttatcgc tcctaaaata atttttggaa
     1081 cgaaaaaagg acgagtggaa tcgcaaaata aatttcgcct gtatccattc gtccattttg
     1141 ttttgtattc aaaatcaaaa attgatatac taatcatcga atgaataaca agtgtggttt
     1201 gttattccgg gcgagaacgt tggtagcgtt gctcgtcttt ttttatgtct ttttttcgtt
     1261 aaaactattt taaaactaaa tatatcttaa agcaaataaa ttcaaggggc taaaagcgcc
     1321 ccttaaaaac cccttgcctc tgaaaagttt tgtgtttcct ttccgtcggt ttcaaaagct
     1381 aatttactca aaaaaataga tcaagggtaa atgcactcac tgcgttcgcc cttgagctat
     1441 tttatttcgc aaattttaac gcatttgccg acgaaaagat aaaacacata aaaatatacg
     1501 cagatatatt taaaacgtct tagaacgcaa aattttacgt ttaaaagcta tgcaaggact
     1561 gtatgaagca aaattattga gttatccaag aacagataca ccatttatta cagagaacga
     1621 atttgcttat ttaaaagcga attttggcaa atatagcggt tttttaggac ttgatcttga
     1681 aatggttcaa acagagccta gaaagcgtta tgtggacggt agtaaggtac aggaacacca
     1741 cgccattatc ccaacaaaac aagtacctac cgaatctgca ttagcgaaaa tggacgattt
     1801 acaacgaaaa atttatgctt tagtcgttaa aacgaccgtt gccatgtttt tacctgatta
     1861 cttgtatgaa gaaaccaaga tacaaacgaa agtagctgat ttgctgtttc aatcgatcgg
     1921 aaagacacca aagcaagaag ggtggaaaat tcttttcaaa caacaaacca aagaagaaaa
     1981 agaggacgtt caaacgttac cactcgttat cattggcgaa cgtgccgaag ttggtgttaa
     2041 gagtgttgaa aaagaaacgc aaccgccaaa agcttttaca gagggtacat tattaactgc
     2101 tatgaaaacg gcgaataaaa cggttgatga tgaagaagca atcaagattt tacaagaagt
     2161 tgaggggatt ggaacagaag cgacaagagc aagtattatt gaagcgttaa aacaaaaaga
     2221 atatatccaa gtgattaaga ataagcttgt tgtaactgaa aaaggaaaat tattgtgcca
     2281 ggcagttgaa agtcagcacc ttttaacgag tgctgaaatg acggctaaat gggaaacgta
     2341 tttaaaaaaa atcggtaaaa gagaaggcaa tcaagagaac tttattacga atatcaaaaa
     2401 attcattgtt catttactgg aagctgtacc taacgatata gaaaaactaa atttttctga
     2461 ttaccaggaa cagaaagaaa aagaagcaga aaaaagtatt gtaggaaaat gtcctaagtg
     2521 tggcaacaat attgtattaa aaaaatcgtt ttatggttgt tcaaattatc ctgaatgtaa
     2581 gtttacttta gctgaacatt ttagaaagaa aaaactaacc aaaacgaatg taaaagaatt
     2641 actggaggga aaagaaaccc tggtaaaagg aatcaaaaac aaagagaaaa agccctacaa
     2701 tgccgttgta aaaattgggg aaaagggata tattgatttt atatctttct caaaataaac
     2761 ataaaagccc tttaaagagg gcttttatat attaatcaca aatcacttat cacaaatcac
     2821 ttatcacaaa tcacaagtga tttgtgattg ttgatgataa aataagaata agaagaaata
     2881 gaaagaagtg agtgattgtg ggaaatttag gcgcacaaaa agaaaaacga aatgatacac
     2941 caatcagtgc aaaaaaagat ataatgggag ataagacggt tcgtgttcgt gctgacttgc
     3001 accatatcat aaaaatcgaa acagcaaaga atggcggaaa cgtaaaagaa gttatggaaa
     3061 taagacttag aagcaaactt aagagtgtgt tgatagtgca ttatcttaaa attttgtata
     3121 ataggaattg aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca
     3181 tgttggtatt ccaaatgcgt aatgtagata aaacatctac tgttttgaaa cagaagtcaa
     3241 gtccataatc ctgtgtaaaa tactatacaa tgttttaccg gtctctcatt gatcaaactt
     3301 aatatatttt cttgtagaac tgagccattc gtcatgaagg tccataagga cagctccaat
     3361 taatcgattg gcagacgttc ggttgggaaa aatccgaata atcttttctc ttctgcggac
     3421 ttcctggttc agtcgttcaa gaaggttggt gctttttagc cgattatgac tatttccgat
     3481 aaccgtgtat tgaaaggcat cttcgaagcc attatccaat atttcgcagg cttttgtata
     3541 tttaggctgg tcgatatatt caccgactaa ggcattctta gctgttcgag cgagttcaat
     3601 atccgtaaac ttaaagatcg cttttactgc ttctctaaac ggttttgaat tcttttttgg
     3661 aatggaactg aagatgtttc ttaaaaaatg gacctggcat ctctgccaac ttgcgttggt
     3721 aaatgactta cgaatcgcag acactaggcc tttatgggca tcagaaatga tgagttctgt
     3781 cccctttagg ccgcgttctt ttaagtattc aaagaagatg gaccatgtgt catcactttc
     3841 ttcattttga atcatgaagc caatgatttc acggtcgcca ccttctgtta tcccgatcgc
     3901 aatatggcag cttttagaaa gcactcgatg gtcctcacgg acttttatgt agagaacatc
     3961 agtcatcaga taaggataat tcgtacctga gagtgaacgg ttctgccatt cgttgaccat
     4021 cgggtctaac tgctcagtca ggctagaaac aaaagatttc gatacagatt ttccacatag
     4081 ctcttcaaca atctttgaaa ctttacgtgt cgaaacgccc gaaacataca tctcaagcat
     4141 tgaagcgagc agtgcctttt catttcgctg ataacgctca aacaccgtcg gtgaaaattc
     4201 accatcacgt gttctaggca cttttaattc gagtgtaccc acacgagtcg taaagtctcg
     4261 ctcataatag ccatttcttt gactctgacg actttctgaa cgttcatagt catcggcttg
     4321 aatatattct gttcgttgat tttccatcaa ttggttgaaa acagtggtta aaatattttt
     4381 agaaacatca tcttttaccg aatgttcaat aatactttga acctcttcgt tgttcagtgt
     4441 aaaatgtact tgggtcatgt aacgtcctcc tgggtatgtt tttgtggtta aaaacattgt
     4501 accgtaaaag ggctgttaca tggcctttta tcttttacac aattatatgg actttatcga
     4561 aacagactaa aaacagtgat tacgcagata aataaatacg ttagattaat tcctaccagt
     4621 gactaatctt atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct
     4681 gtatttgttt atagatgtaa tcacttcagg agagattaca tgaacaaaaa tataaaatat
     4741 tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta
     4801 aaagaaaccg ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg
     4861 gctaaaataa gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg
     4921 tcagaaaaat taaaactgaa tactcgtgtc actttaattc accaagatat tctacagttt
     4981 caattcccta acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca
     5041 caaattatta aaaaagtggt ttttgaaagc cgtgcgtctg acatctatct gattgttgaa
     5101 gaaggattct acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact
     5161 caagtctcga ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa
     5221 gtaaacagtg tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg
     5281 aagctatata cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact
     5341 aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccattact
     5401 tatgagcaag tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc
     5461 tatgagtcgc ttttttaaat ttggaaagtt acacgttact aaagggaatg gagataaatt
     5521 attagatata ctactgacag cttccaagaa gctaaagagg tccctagcgc ctacggggaa
     5581 tttgtatcga taaggggtac aaattcccac taagcgctcg ggaccc
//
LOCUS       DAJSKS010000150         5622 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJSKS010000150 DAJSKS010000000
VERSION     DAJSKS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.155"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            2..667
                     /locus_tag="ON396_002471"
     CDS             2..667
                     /locus_tag="ON396_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114518.1"
                     /translation="MAYYLLSEPKQIAASNIFTAVMTLMCVMCFSIFSYLLVQFIRQN
                     LTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIKNFLTTIDQFINGYMYLFEVG
                     FILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVDFGKYEELIHTDIVHTSIHEV
                     TVKGIRSTLKDGQETKTIDQRKEVYNFYKVEASLRYGIGKLRLDGSNIQQNKPYVLGK
                     VVI"
     gene            711..980
                     /locus_tag="ON396_002472"
     CDS             711..980
                     /locus_tag="ON396_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114519.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            1212..1508
                     /locus_tag="ON396_002473"
     CDS             1212..1508
                     /locus_tag="ON396_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114520.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            1510..1932
                     /locus_tag="ON396_002474"
     CDS             1510..1932
                     /locus_tag="ON396_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114521.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            1951..3543
                     /locus_tag="ON396_002475"
     CDS             1951..3543
                     /locus_tag="ON396_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114522.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            3564..3884
                     /locus_tag="ON396_002476"
                     /pseudo
     CDS             3564..3884
                     /locus_tag="ON396_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4045..5186)
                     /locus_tag="ON396_002477"
     CDS             complement(join(4045..4958,4958..5186))
                     /locus_tag="ON396_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR3114523.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            5434..>5622
                     /locus_tag="ON396_002478"
     CDS             5434..>5622
                     /locus_tag="ON396_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114524.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYPF"
BASE COUNT         1986 a          892 c         1129 g         1615 t
ORIGIN      
        1 ggtggcctat tacttgttaa gtgagccaaa acagatcgcg gcgtcaaata ttttcacggc
       61 agtaatgaca ttaatgtgtg tcatgtgctt ttctattttt agttacttac tcgtccaatt
      121 tatcaggcaa aatttaacaa tgagtaaact tttcaaaagt ttattggact tacttatctt
      181 aggcagtatg ttaacgactc taacagatcg gttcgaacaa tctgaaaaaa cggtagaggg
      241 gataaagaac ttcttaacga cgattgatca atttataaat ggatatatgt atttgtttga
      301 agtaggcttt attctcactt tgtttggttg tgtatttata aaactcatta aaagatcaat
      361 cgaaaaagaa atggttattg atctaattcc gacgctgagc ggctatgagt attacgttga
      421 ttttggaaag tatgaggagt tgatccatac ggatatcgtt cacacgagta tccatgaagt
      481 aaccgttaag ggcatcagat ctactttaaa agatggacag gagacgaaaa caatcgacca
      541 aagaaaagag gtctacaatt tttataaagt tgaagcaagt ctacgatatg gcattgggaa
      601 attgaggtta gatgggagta atattcaaca aaacaaacca tatgttttag gaaaagtggt
      661 gatttagaac ttcctatgaa agatgaataa ataaggagaa tcttttgaaa atgattcttc
      721 tttacagaat tgtgcttata gttggcacgg agttaaaaat cttaaagaag gagcagttat
      781 tcaaagtgaa agaacatcat aatcagttac tatgtattaa aacgggtacg atgaacattc
      841 aataccgaac agaatattta gaattaacga atcaaactga ttttttgtcg attcgagagc
      901 aacataagca agaaaaatta atggctactt atagccaatt gattgatgag tgggaacaat
      961 tcaaggaatc taaaacataa aattccattt aaacacctta ttttttaaag agcataagga
     1021 aacgtgaccc cacttttaaa aaaagtggtt ggataggctg tgcctatcac gtttctgaaa
     1081 aaaataaatg accccaaaat accaatatat aggatagtac aaggatagca aaggatagta
     1141 aaaggataga ggtaggatag cgaaaaggat aggatccttt tcactatcct atattatatt
     1201 ggaggatatt tatgaatgtt aaaattagag atttagatcc aaaattcatt tctgaaattg
     1261 atcgaagatg cgttgaacta agtaatcgta caggaaataa atggagtcgt aatgattatt
     1321 taaagctact cgttgaaaat gattttgatc gtcctttgat ggaatacaaa caagaaaagt
     1381 ttgaccagtt attagaaaag ttttcagaaa ttcaaagtta taacacaaag atattagaag
     1441 aatatgttag ccaaaataat cgtattatag aaatcttgat tgagcaaaat agaggaagtg
     1501 aactttgata tggcaaaggt tatagacaaa cacatttact ttcaccaaac acatatcgat
     1561 gtattggatg aaactatttc tactcatgca gaggttaaaa actatagtga agctgttcgc
     1621 tttttatgta tgaactatga tccagaagtg gagaaaaaag taaattccaa tcaagcaaag
     1681 ttgaatggaa tgagtaaaga aatttctatt ttaaccgaaa tggtttcaga atttgcggat
     1741 cttcatttga aagatacagg aatattaatt ggtaaaaatt caactgttta tcaagaagca
     1801 aaagaacgag tggaaggaaa aattaaatcc aatcaaacaa aaaaatattc ccctcagttg
     1861 aagaaacctg tgaatgaatc tgttcatgcc agagtagatc catttgatcc aacgaagatt
     1921 ctaaaaagat aaaaatgaag gaggataata atgactcaat cgccttcagt tattctcact
     1981 tgtcaattta ctttgccgaa taaaaagtca ttttctactt atattgacta tattacaagg
     2041 gaacaggctc ttgaagaaaa ggaacgacgt acgcccgaag aagaacagga acttcaacga
     2101 atcaagaatg ctttagagga gttttatatt cctgaaggag aaactttttc ggagagtaag
     2161 acgggtaaag aattcagtga aacgtcgaat gaagctcaat cgttgatgga acatggaatc
     2221 aattttgaca agttagagga acaagatttt accaattatc tttcttatat gacacgtcac
     2281 tatgcgttag aacaaaagaa agaactgacg caaagtgaac aaaaagaata ccaacgcttg
     2341 aaccaagcaa tgaaaaaata tcaaacagaa ccccataaaa aagcttcagg aaatcttcct
     2401 ggagtttttt ctatagcttc agacgaagtg aaaggaagtg atctgaagga gattaagcaa
     2461 atcttccaaa gaggacaaca aaaaggctca atcatttatc aagatgttgt ttctcatgat
     2521 aattcgtatc ttgaaaaaat aggattgtac gatcccaaaa cagatacttt agatgaagaa
     2581 gggttaaaag cagctggaag aaaaatgatg gagacattat tcgagaaaga acaattgaat
     2641 gaaacaggtt actggatggc aacgattcac cgtaacacta aacatatcca tattcatttt
     2701 gcagttgttg aaaaagaaaa tacgcgtaaa ccttacacgg aggttgaaaa tggtgtgagt
     2761 tatatcgtac cgaaaggtaa gagaagacaa gcaacattag acgcaatgaa gaccaattat
     2821 gtacatgagt tagaacgttt ttcttatgaa aaggatcgaa tgattggtat ggagaaacga
     2881 aatttattga cgcggaaaag tgatttgcgt aacaccttaa caaaagtggt aaaagagcca
     2941 gcgcgttacg atcgacacgc gatgaactta ttaaatgaag tatataaaac cctacctgac
     3001 aaacgttcgg aatggaatta cggggatgaa aaacgaacca aactttcacc atacacaaga
     3061 gagaaactcg atgagttaac caaatatgtt ttaacaaaag aacctgaata tcaagaatat
     3121 atacagattt caaaagcatt gaaagaagaa gcaaaaagtc tttatgggga aagcaagaga
     3181 gaatcaaagg atgcagagaa aaatgctctc tttgatttaa aaaagcgcac aggaaatgcc
     3241 atcctaacag agctgaagag acaggatagt caaataaaac cattaataga aagtgttgat
     3301 cagatgcaat ctatttacaa tgaacgatcc tttcgaacgc ttaaacaatt gccaacgaat
     3361 gaatacaaat atagttacga agaattcatg aagcgcagga aacaattcca acaaagagtt
     3421 gtcacgcagc ggagtttgcg tcaattaaat cgaacgatta atgcccatga agaaaaatac
     3481 cacgcaagta aagcatacga agagcaacaa cgaagaattc aaatggaaca agaaagacag
     3541 taaagacagt aggaggaata gcaatgaaac aatatttaga tcaatggaaa gttatagaag
     3601 gatcattaag agaagaacga atcgaacaac ttcctgattg tttagaaaaa gagcatttgt
     3661 tccagataag agaaatgttg agaaacgaac agtttgatcc aaatcaattt ttagtcgttg
     3721 agtacccagc tactggtgtg tattgttgta atcatgtgaa tggtgaaaaa tattttatca
     3781 ttcaagaata tgagggcaag ttagcacctt attatactac ttgggaaatg aatgaagagg
     3841 gaatcaataa ctttccatgt aaaagcattg gatttgagtc aatacctcgg acacgatttg
     3901 tgagaatttt ttcgagattc cagcgggtga gtatctcatc gtttcgacca gcggtcaaac
     3961 caaaaccgtt tgaccgctga tcgaagcgat gaagttgaaa ccctgtggaa atctcgccaa
     4021 aaaaatgtaa gtggtagcta ggaactaagc ttgcaatgcc aactcctcca tctgatttgg
     4081 tgttaaatag ttgatactac tatggattcg cttgcgattg taaaatcctt caatgtattg
     4141 aaataacgct aaataagcag cttcaaagtt ttcatacgct tttgatggat agacttcttc
     4201 ttttttgagt gaggcgtgaa acgattcgat accggcatta tcgtagggac agcccttacg
     4261 actgaaggag tgccgaatgt tcaattcatt cagctttgcc tcataggctt cgctagtgta
     4321 ctgagaaccc aaatctgagt ggatgatcaa gttctcggag ggtgtttggt ttaagagcgc
     4381 attgtcaagg gtttcgataa ccaattcttt cgtcatctgc ttgccaaact tccaaccgat
     4441 gactttcttt gtgtggaggt cttgaatagt ggagaggtag gtccagccat cttttagggt
     4501 atgaatatag gtaatatctg tcacccattt ttgattgatc gacgtagtgg taaaatcttg
     4561 gtttaacagg tttttacgtc cttcactttt aactttcgct tgtttgccag gcttgtactt
     4621 cttgagggtg actgaacgca agcctaattt tctcatcagt atttgtactt tctttaagga
     4681 caaggtctcg ccacggccaa caagaatctt atgaatttta ggcgcaccat agataccctt
     4741 gttttcaaag tagattcctg aaatcaagcg gtttaagcgt ttgttttctg attggcgctt
     4801 ggaaggtttc tttttcttcc agtcatagta gctggatttc ggtgcttcca aaacggtaca
     4861 catttgtttt acgtcatgat catgaacatt tgattgaata aattcgaaga catcactcat
     4921 ttttactttt gagcgaatat ggttaaagcc ttttttagga tagtgttttc ctcctgcatg
     4981 cgggccattt cctttttcat cgctaggatt tctgcctcag tcatgccggt tgctacattc
     5041 tttgtatgaa gcttcttcca acgataaatc gtttgtgtag cgataccata ttgcgctgcc
     5101 agttcttcga ccgattggcc agactggttt aaatctaaga tcatcttctt aaagtcttca
     5161 tcaaattttt tcttggcttt tgccatcgtg gacacgtcct tttcttagta gttagattct
     5221 accaaatcgt gtccatgaaa tcatcctaac atcacattga agagtccatt tcactcacag
     5281 agtgttagtt gttaagtgaa taaaagaagt aaaagagtcc tgaacgtgat tgtccaggac
     5341 tctttttttc aatgaattgt tttccgccaa ctcattgagt ggtgcgattt gcgactacat
     5401 tatatcaaaa gaaagagaag ggaggaagca gaaatggttc attcaacgat tggagttgtt
     5461 gttgaaaaaa gcagagacaa tctcgttttt gtgacagaaa ttcaaacagg cagatctttt
     5521 gtcgtcacag acaaagcggc aaaagcttat caaagtagcg atattttaac gttaaatctg
     5581 actacgaaga cgttcgtcga tgcgacagaa gattatccat tt
//
LOCUS       DAJSKS010000151         5509 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJSKS010000151 DAJSKS010000000
VERSION     DAJSKS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.156"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            27..845
                     /gene="purR"
                     /locus_tag="ON396_002479"
     CDS             27..845
                     /gene="purR"
                     /locus_tag="ON396_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCR3114525.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1348..2721
                     /gene="glmU"
                     /locus_tag="ON396_002480"
     CDS             1348..2721
                     /gene="glmU"
                     /locus_tag="ON396_002480"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR3114526.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2815..3786
                     /locus_tag="ON396_002481"
     CDS             2815..3786
                     /locus_tag="ON396_002481"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR3114527.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3792..3894
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3960..4529
                     /gene="thiT"
                     /locus_tag="ON396_002482"
     CDS             3960..4529
                     /gene="thiT"
                     /locus_tag="ON396_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HCR3114528.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4610..5383)
                     /locus_tag="ON396_002483"
     CDS             complement(4610..5383)
                     /locus_tag="ON396_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114529.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
BASE COUNT         1800 a          979 c         1160 g         1570 t
ORIGIN      
        1 aaatttctta aaaggagttg gaataggtga aagtacgtcg cagtgaacgt ctagttgaca
       61 tgacacagta tttattgaat cacccgcatg aattgatttc cttaacaagc tttgccgaac
      121 gttatgaatc tgctaaatct tctatcagtg aagatttggg aatcatcaag aaaacattta
      181 aagaacgagg gtatggaacc ttggaaacaa tcccgggtgc tgcaggtggt gtattgttca
      241 tccctgaaat ttcatatgaa gaggcgaaaa aatatattga atctctagca gaacgtctat
      301 cagaacaaga ccgtctgctt ccaggaggat atgtttatct ctctgatttg ttaggagaac
      361 ctgaactgct gcgtcaagtt ggaagaatca ttgcttctaa ataccttggt gaaaaaatcg
      421 atgctgtaat gactgtagcc acaaaaggcg tacctattgc ccaagctgtg tcctattatc
      481 tgaatgcgcc attcgttatt gtacgccggg actcaaaaat aacagaagga tcaacagtaa
      541 gtgtcaacta tgtgtctgga tcttctgaaa gaattgaaaa gatggaactg tctaagagaa
      601 gtttgaaacg tggttcacgt gtccttgtag tggatgattt catgaaaggc ggcggaacag
      661 tcaacggaat gaagagcttg attgaagaat tcgaatcaga acttgttggc atcactgtat
      721 ttgccgaatc aaaattcaac ggtcatcgcg ccatcgatga ttatacgtca ctgctttatg
      781 tcaaagatgt agatactcaa acgaaaaata ttacggttgt gccaggaaat tatttttcag
      841 aataagaggt tgtgaaaaag ccgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
      901 gctttccaca ttttttcata aatttggctt aataccgaga aactggcttt tgaacaccgt
      961 ttatctagag gttgtgaaat cagcgtcctg tcctgagtat tagctcaata aacggaaaaa
     1021 ttgctcctca tattttgccg tttattgagc taataccgaa ggtcagctga ttgaaaaacg
     1081 tttaatgaag aggttgtaag cctaccgttt aactctaagt cacaagaaac gatttcaaaa
     1141 aacagctttt catgttttat gagtagtgtg acttgtgacc gaggagttgg caggagagca
     1201 ccggttacca aatagatagg cccgttatgg tctatctatt tttttatcca gaaaacgctg
     1261 ggataaaaga aaatcacttg ttttgttgaa aaaatgggta tactgggata aactgaaatc
     1321 agcaaactat atttaggagt gacgcagttg gacgcacgtt atgcaattat tttggcggca
     1381 gggaaaggta cccgcatgaa gtcaaaatta tataaagttt tacatcccgt ttctggtaaa
     1441 cctatggtag aacatatcat taatcgggtc agtgaaacga aaccagatga agtgattacg
     1501 atcgttggac acggagcaga acaagttaaa gcacaactag gagaacgcag taaatatgcg
     1561 ttacaagcag aacaattggg aacaggacat gctgttttgc aagctgcttc tttcttggaa
     1621 ggaaaaaaag gaacaacgtt agttatcagt ggcgatacac cattattgac tactgaaacg
     1681 ctgaacaatc tttttgaata tcaccaagga aaaaatgcaa gtgcaacgat cttgacggca
     1741 caagcagaaa acccaacagg atatggacga atcatccgtg accatattgg gatcgtagaa
     1801 aaaatcgtgg aacaaaaaga tgcaacacca gaagaagctc ttgtacaaga aatcaataca
     1861 ggaacttact gttttgacaa cgaagctttg ttcgatgcgt taagcaaagt tggaacaaac
     1921 aatgctcaag gtgaatacta tctaacagat atcatcgaaa tcttaaaaga agaaggccat
     1981 acagttgctg cttatcaaac ggatgatttt gaagaatcaa tgggagtcaa tgatcggatc
     2041 gcgttagcta aagcaaatga gatcatgaga aagcgaatta accagatgca tatggtcaat
     2101 ggcgtaagct ttgtcgattc agcaacgaca tacatcgatg caggcgtaga aatcggtcca
     2161 gacacattga tcgaagcagg tgtacaaatc caaggaaata cagtcattgg ttctgattgt
     2221 gtcatcgggt cacattcaaa aatcgtagac agtaggattg aggatcacgt cgtcattgaa
     2281 aactcagtca ttgaaagcag ccatgtcaag aaacatgcag atgtgggacc ttatgcacat
     2341 ttacgaccaa aagctgaaat tggcgaaaac gtccatatcg gaaactttgt ggaagtgaaa
     2401 aatgctcaaa tcggcaaagg aactaaagtc ggtcacttga cttatgttgg tgatgctacg
     2461 ctgggagaag aaatcaatgt cggatgtggt gttgtcttcg tcaactatga tggaaagaac
     2521 aaacatcata cgactgtcgg cgaccacagc tttattggtt ctagcacgaa tatcatcgga
     2581 cctgtcgagg tagctaaaaa cagttcgatt gctgccgggt caacaattac agacaatatt
     2641 ccagaatatg cgctggcaat tgcgagagca agacaagtca ataaagaagg atatgcaaaa
     2701 aaactccctt atctaaacta agacttttgc aaaatcactt gatttattta aaaaatatga
     2761 ctactattta ttaggtagaa ttctagagtt gtgaaaaaga aagtggaggt ccccatgtca
     2821 aaacattact ttgacccaag attaaaaatt tttgcgttaa attccaatcg cccattagca
     2881 gagaaaattg cagcagcagt cggcgtcgag ttaggaaaat catctgttac tcaatttagt
     2941 gatggagaaa ttcaagtaaa cattgaagaa agtattcgtg gatcgcatgt ctatgttatt
     3001 caatcaacga gcagcccagt caatgataac ttgatggagt tgctgatcat gattgacgca
     3061 ctgaaacgag caagtgcaaa aacaatcaac gtagtcatgc cttattatgg ctatgcgcgt
     3121 caagaccgta aagcacgtgc acgggaacca atcacagcga aattagtagc aaacatgatt
     3181 caaaaagctg gtgcaacaag aatgctgact ttggaccttc atgcagtcca aatccaaggt
     3241 ttctttgata ttccagtcga tcatttgatg ggtgcgccac ttattgccaa ttatttctta
     3301 gaaaaaggaa tcaaaggcga tgatgttgtc gttgtttcac cagaccatgg tggtgtcaca
     3361 cgggcacgta aattagcgga atttttgaaa tctccgattg cgatcataga caaacgccgt
     3421 ccaaaagcaa atgttgcaga agtaatgaat atcattggac aagtcaaagg caaaacttgt
     3481 gtactgatcg atgatatgat cgatacagca ggtacgatca cattggctgc caatgctttg
     3541 aaagaagctg gtgcaactag tgtgtatgct tcatgtacac atccagtatt atccggccct
     3601 gctttacaaa gaatagaaga ttcagctatc gagcgtctag ttgttacaga ctcgatctat
     3661 cttccagaag atcgtaaaat cgacaaaatc gatgaagtaa gtgttggcgg actaatggga
     3721 gatgcaatca aacgtatcca tgaaaacaaa ccagttagtc cattgtttga aacaaaaaat
     3781 aaataataaa taaactcaca aaggggagtc caattgggct gagattgaat ctatttctaa
     3841 acccttcgta cctgtatcgg ttatgcgagc gtaggaattg tgaataaaca gctgtttgac
     3901 tattttcgaa agctgcttcc tcctttgtga cttttttatt caaaggagga agttttttta
     3961 tgggaaaaaa tagagtttgg gtagaaggaa cgattgttgc agcgttagcg atggtgttgt
     4021 cgttgattcc gatccagatc ggcagcagtt tttctatttc attagggcag attccattga
     4081 ctctttttgc gttaagaaga gggacaaagg cagggatgct ggcaggtttc atttggggca
     4141 tcctacattt tccgttggga caagtttatt atttaagtgt cgttcaagtg ttgattgagt
     4201 acccgattgc ctatactttt gcaggcgcag cagggctcat ggcttcaaat gtccaaaaag
     4261 gctatgtaga cgataatacg aaacaaatta gaaaaagcat cgtgtttggt gcattgattg
     4321 gcgcactttc cagatatttt tggcatttta ttgcaggagt ggttttctgg ggagcgtatg
     4381 cactatgggg gatgaatcct tggctctttt ctttcgtaat gaatggtgcc agcgggttga
     4441 gtacagctat tgtaacgatc atcgttttat tagggatcca acaaacgact ccttcgattt
     4501 ttgtgccaga taaacgacag gagctgtaaa taaaaaagaa gcaagaaact tgagacagaa
     4561 tcatgtccta gtttcttgtt tcctttttat tttttgataa gaaatagatc tacttcaatg
     4621 gcagataaga aagatcggca tcttctacca attgccagtg tcgatcggtg acagagtaga
     4681 ttaaagtagc gatgatttcc tcttctcgat accgggtgcc ttttaatttc caaatataat
     4741 cttcaatcac atagattggg atacgatatt taccatcgat caaaccaact tgatcaaaag
     4801 aaaaaatgat cccttcctgc atcaaattac gcatttcttt caataattcc acactgattt
     4861 tttcaacagg tgtttctttt ttgaaaaaag atttttttac attgatctta tctgacaaga
     4921 tcgaacttaa tttactagca aaaatcctat aaaattcatc taaatagcga taataaatcc
     4981 gattcagctg atcattaaga tcgaagtata caaaattatt ctgtagttta taaaaaaacg
     5041 gcgaatgaag atgtgttttc atatgaccaa aatataataa ttctgaaatt tctaaaggtg
     5101 tcaattcttt cagcatcgtt aaatcagtaa agtcgatcca cttcaattca tcgaatccac
     5161 gcctgttacg agaagaggaa aagaaacgct gaatattttc tgtaccacgg attactttca
     5221 gccctgtatg ggattcatat tctccagcag aagcagatgg atcaagcaag agcaagtttt
     5281 gaggaaaatg cacgatactg tttgcaaagt cagtagcagt cagccctttt gttaaaacag
     5341 cattacttgt ggtatcaata tgtacataaa ctgactcagt catcatgttc ctcctcttat
     5401 ttcctcttaa tctcttttat tttacaacat ttgcattaaa aaagcttgtt tcttttattt
     5461 taaaattcca ttacaaaatc acgttttcat tgaaaacact ctcattttc
//
LOCUS       DAJSKS010000152         5482 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJSKS010000152 DAJSKS010000000
VERSION     DAJSKS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.157"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            61..1692
                     /locus_tag="ON396_002484"
     CDS             61..1692
                     /locus_tag="ON396_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR3114530.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1695..2774
                     /gene="uxuA"
                     /locus_tag="ON396_002485"
     CDS             1695..2774
                     /gene="uxuA"
                     /locus_tag="ON396_002485"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR3114531.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2848..4275
                     /gene="uxaC"
                     /locus_tag="ON396_002486"
     CDS             2848..4275
                     /gene="uxaC"
                     /locus_tag="ON396_002486"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR3114532.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            4359..5006
                     /locus_tag="ON396_002487"
     CDS             4359..5006
                     /locus_tag="ON396_002487"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HCR3114533.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            5126..>5482
                     /locus_tag="ON396_002488"
     CDS             5126..>5482
                     /locus_tag="ON396_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3114534.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYI"
BASE COUNT         1883 a          743 c         1160 g         1696 t
ORIGIN      
        1 caatgggttt taaaatataa aatgtatcaa aactaacata tcagtttgag gaggttaatt
       61 atggtttcga ttaaaaattg gcaaaattct atcgaagaat tcaatgaaat gcagattcta
      121 ctgccgtgtt ttgatgtcaa agaagttaag caagaaggcc agcaacatcc agtatggctt
      181 cattttggtg gaggaaatct ttatcgagga tttcatgcag aaattgctca atgcttagca
      241 gatcaaaatg aattagaaac aggagtagtt gtgtgtgaaa catttgatga agaagtagtg
      301 gaaaaagctt atcatgacta taacaatgac attttggaaa ttgttatgag tggagatggt
      361 gaactcaaaa aaagactttt ggcagtaacg gctgatagtt tatattgcaa tccgaagaat
      421 atagcggact atcaaagagt ggttaaatat tttgaagatc ctaaattaca gtttacaacg
      481 tttactatta ctgaaaaagg ttacagttta aaagattcta aagggcaatt cttttcggta
      541 gtcaaagctg atatcaaatc tggacctagc aatcctcaac atacgatgag tattgtaaca
      601 tccttgttat atagcagatt taatgctgga gagttgccga ttgcgatggt ttctactgat
      661 aatttctcac agaatggtca acgttttcaa gatagtattt ttacgattgc agaagcttgg
      721 caaaaagaag gatatgtttc tgaaaaattt attgcttatt taaaggataa agaaaaagtg
      781 acttttcctt ggagtatgat tgatcgaatt acgccaaatc cttcagagaa tgtaaaggaa
      841 gatctattga tgatggggct cgaggattta gatatcattc acacaaaaaa acataccaat
      901 attgctgctt tcactaatac tgaggtagtt cattatttag ttattgaaga tagttttcca
      961 aatggccggc ctaaattgga aaaggctggt gtgattatga cagatagaga gacggttgat
     1021 aaagcagaca tcatgaaggt tacgacaagc ttgaatccac ttcatactgc tttggcagta
     1081 acaggttgtc tgctaggata tcaatcaatt tcagcagaga tgcaggatga tgatttggtt
     1141 ggtttaatca aaggaatagg ttatttagaa ggtttaccag tagttgaaaa tccaggcatt
     1201 attgatccaa aacaatttat tgatgaagta attaataaac gtttgcctaa tccctatatt
     1261 ccagatacac cacaacgaat tgcaacagat acttctcaaa aagtagcgat tcgctatggt
     1321 gaaacaatta aaaagtatgt cgaaaaagac ggaaatgcag aaaatctaac atttattcct
     1381 atcgctattg ctgcttggtt acgttattta ctggcagtcg atgatcaagg ggaaaatttt
     1441 gtgccaagtc ctgatccgtt attggtagag ttgcaggata aattatcagt attgaaatta
     1501 ggtgaacaag atccttcaaa gattcattgt gcggtacagg aaattttaga aaataaagaa
     1561 atctttggtt tagatttata tcaagttggg gtaggaaaga aaactgaaaa aatcttttgc
     1621 gaaatgctag taggtcctca agcagttcgt aaaacaatca ataaatatat aacagaagtt
     1681 ggaggaaagt aactatggaa atgactttta gatggtatgg ggcaaatgaa gaaaaaatct
     1741 cactacaaga aattcgtcag atcccagcaa tgaaaggaat tgttggaaca ttaatggata
     1801 taccagccgg agaggtttgg ccgagagaac gaattaaatc tttaaaagat gaagtcgaag
     1861 ccaatggact taccttgaaa gttattgaga gtgttaatgt tcatgatgat atcaaaattg
     1921 gtttaccaac acgagatcaa tacattgaga attataaaca gacgattcgc aatttggcag
     1981 aatttgggat tgaggttatc tgttataatt ttatgccagt atttgattgg gtaaaatcgg
     2041 atttggatta tcgtttagat gatgggtctt ctacgttagc ctatatttca aaaaatatcc
     2101 ctactgatcc ggaggaaatt attagagaag tagaaaattc tactaataat tttgaattgc
     2161 cgggttggga acctcatcgg ttatcacaaa ttaagcaatt attagaagcc tataaagaag
     2221 ttgatgaaga gaaattacga gagaactttg catattttct caaagcaatt atcccgacat
     2281 gtgaagaagt tgggattaaa atggcagttc acccagatga tccaccatat tccattttcg
     2341 gattacctcg tattgttaaa aatcgtgacg atttagattg gttgtgtaat gtagtggatt
     2401 ctccttctaa tggtattaca ttatgtactg gaagtattgc agaacgacca gataatgatg
     2461 tctatgaaat tctatctgaa ttttgtcaac gggatcggat tccttttgca catgttcgta
     2521 atatcaaata tttaagtgac aatggactcg atttttacga agcgccgcat aaatcagaat
     2581 atggttctct agatatgtat aagatattaa aaacaatgta cgataatgga tttaatggct
     2641 atattcgtcc agatcatggt cggatgattt ggggagaaac cggtagacct ggttatggat
     2701 tatatgaccg agctctaggt gcagtttatt taaatggtat ttgggaagcg cttgaaaagg
     2761 gggcgagtaa ataattatat aagggagttt atttagagga aaaattttct cttctaaaaa
     2821 ctaacggaag gaaaggagtg tttaaatatg tttttagata agaacttttt attagagaat
     2881 gaaatagcta aaagactttt tcataaattt gcaaaacacc aaccaattat cgactatcat
     2941 tgtcatttag acccagcata tttgctagaa aataagcaat acaagaattt gactcaggta
     3001 tggttaaatg ataaaggagc tggagatcat tataaatggc gattgatgcg agctaatggt
     3061 atagaagaag agttgatcag tggtccaggt gatgattatg aaaaattttt agcttttgta
     3121 aaaacagtgg agaaggcgcc aggtaatcct atttttgaat ggtctcattt ggaattacaa
     3181 cgttactttg atattgattt gacaatttct gagaagaatg caccagaaat ttggcgacgt
     3241 gctaacgaaa aaattcaaac ggattcctat cgcccgaaag aattgttgga gatgatggat
     3301 gtgaaagcca tctgtacaac ggatgatcct acagatgact tgtggtatca tactgagctt
     3361 tctaaacagg ataattcttt caaggtatta cctactttcc gtccagataa ggtctggagt
     3421 atcaaatcat caggcttttc gaactatatt ttaaaactta gtcaaacagc ggctatacca
     3481 atcaaagact ttaatgggtt gaagcaagca ttgagccaac gagtggatca ttttcatcaa
     3541 attggctgtc gattagcaga tcacggtttg aatacttatt tttatgaaat agcagatgaa
     3601 aaaatactcg acaatctgtt aataaaagct ttagcaggaa aaacggattt ccatttatca
     3661 gaaattgcac aatttgttac aggtattcaa ttacatttga tgtctgagta tacaagatat
     3721 ggttggacga tgcaaatgca tttaaatgct ttaagaaata attcaagtat attaaaggaa
     3781 caaattggag tagatgtcgg tggtgattcg atgggggatc aagctagttt gacacaaaat
     3841 gttgtagcat tgttcgatac cgctgaaaag agtgggtgct taccaaaaac aattttatat
     3901 tcattaaatc caactgattg gcttccacta gcaacagcaa tgcaaagctt ccaagggggt
     3961 atgattcaaa aaatccaatt gggatgtgct tggtggttca atgatacagc tgaaggaatg
     4021 cgtcaacagt tgactacgat ggcacaacaa agtttgttgt cgaattttac aggtatgttg
     4081 actgattctc gaagttttct aagctatcct cgccatgagt attttcgtcg agtattatgt
     4141 caattactaa gcgagtgggt taaagcaggc cgaattccag atgatgaaat gtatcttagt
     4201 cagatagtgg agaatatttg ttatcgtaat gcgtataatt attttaattt ttttgaaatc
     4261 ggaagtagaa aataatactt gaaataaaag cttaaattta ttctttgtaa gttagtacag
     4321 ctagacaaag tatgtatcca taaaggaagg agatagatat gaaaaaagta gaaattttaa
     4381 gtaagttaga agcggctgga gtcattgcgg ttgtacgtgg gaaaaataag gatgaggcca
     4441 ttaaagcaat aaattcaatt attgcgggtg gaattaaagg gattgaacta acttttacag
     4501 ttcctaatgc tgaagaaata atcaaagaaa ttgctaaaaa atatgctgat caaaaagatg
     4561 taataatcgg agccggtacg gttttagatg cgtttactgc tcgtatcgca attatggctg
     4621 gggcagaata tatcgttagt ccatcttttg atcgacaaac agctgagatt tgtaatttat
     4681 atcaaattcc ttatttacca ggttgtatga caattactga gataaaggag gcacttactg
     4741 ctggcgtaga tatcattaaa ctattcccag gttctgcttt tggtccatcc attgtaaaag
     4801 cctatcaagc tccattacca cacgtaaata taatgcctac tggcggagta agtatagaga
     4861 acttagaaga ttggtttgaa gtaggagttg ttgcagttgg tgtaggtggg aatttattgg
     4921 cttctgttga aaaaggtgat tttgaagaag taagtcgagt agctagtaaa tatatagata
     4981 agttttatga gataaagaat tattaggaga aaaaacaaat aatactctga aatgtaaaaa
     5041 ataaatactg actctttacg cttcagagta tagtacgtac tttcatcaga atgaaaacgc
     5101 ttaacaacag aagggagaga attgtatgga aagaaaattt ggtataagag acaaaatagg
     5161 atatatgttt ggagattttg gaaacgattt tactttcatt tttgctagtt cttttttaat
     5221 gatcttttat acaaaagtat tggggataag cggagctgcc gttggaacat tatttttagt
     5281 agcaagattt atcgatgcag ttactgatgt aactatgggg cgtattgttg atagatcgaa
     5341 agcaactaag cagggtaagt ttaaaccgtg gattttaaga atgagtggtc cagtcgcttt
     5401 agcaagtttt ttaatgtatc aaacttctat gatttcagct tctatgagcg ttaaggtagt
     5461 atacatgtat gttacgtata ta
//
LOCUS       DAJSKS010000153         5346 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJSKS010000153 DAJSKS010000000
VERSION     DAJSKS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.158"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            147..1079
                     /locus_tag="ON396_002489"
     CDS             147..1079
                     /locus_tag="ON396_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114535.1"
                     /translation="MIDYVETQFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEEDK
                     KSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVY
                     KNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLS
                     EKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVS
                     ESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGL
                     ETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1300..3063
                     /locus_tag="ON396_002490"
     CDS             1300..3063
                     /locus_tag="ON396_002490"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR3114536.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3147..3605
                     /locus_tag="ON396_002491"
     CDS             3147..3605
                     /locus_tag="ON396_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR3114537.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3714..4397
                     /locus_tag="ON396_002492"
     CDS             3714..4397
                     /locus_tag="ON396_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR3114538.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4544..5158
                     /locus_tag="ON396_002493"
     CDS             4544..5158
                     /locus_tag="ON396_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCR3114539.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5174..>5346
                     /locus_tag="ON396_002494"
     CDS             5174..>5346
                     /locus_tag="ON396_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114540.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQT"
BASE COUNT         1904 a          842 c         1100 g         1500 t
ORIGIN      
        1 tttttaaaga aaatgattat ttgaaaaata aagaaatcat tatcccagaa tttgactcgc
       61 ggaacatctt gctagcttta gtacgttatg tcaattggga aggctatgaa aataaaaatc
      121 cgtatgtaga agaagcttgt aacgagttga ttgattatgt agagactcaa ttttttaaaa
      181 gaagatattc ggaagatgaa aagtccttta tcatacgaga aatagagatc gcaatcggaa
      241 gaaaggcatt tccccttgga ctgaaagaag aagataagaa aagcgcagaa acgaagccgt
      301 tatttcaatt agtctgtcaa ggattggaag agcaaaaagg aattctagac ttacaagaag
      361 aagatgtcta ttatattttt tctttgttca acagtagaaa ttataccaat gagaacatgg
      421 agctgttaag aaaagatttc gaagttgttt ataaaaattt tgttctcaat gacctatcac
      481 tgaatgaatt gattttacag attattagta tcgctgaaat cgacgatgca gaaaatttta
      541 ttttcaagca atccttttta caatttgtcc gtacgttgtg ggcagatgga caagtatttt
      601 taccggaaag gatctatctt ctatcagaaa aagaacaact tctgtacgga aaaattgtag
      661 aaatattgga agagtggaaa aagaaaaatc aaatccaact aagatggaat gaaaatttca
      721 ttcgaaaatt cgtcaaaagt ctttctttga tcaacagtga acaagcgggg agacaggaaa
      781 tagaaatttt tgttgtttca gaaagttcag tcaaacaatt tttttatcga acccaatttc
      841 atttgtatgc ggaagaaggc atcgctgaaa taaatcctat tttatttcgt tcattagaag
      901 aattgcctga tgaatgtcta tgtgcgaaaa aaagagtggt tttgtgcgat gctgctagct
      961 atcaaaatgg attagaaact gaaaagacaa agatctatcc aatctcatta aaaggtgcaa
     1021 acatccattt gtatcatatt gctcaggaac ttcacctatt aaaaaaacat atttcttgat
     1081 gagaccttaa ttaggagacc ttctacaaag ctttttaatt gtctgtagaa ggtttttatt
     1141 tgagtcctga ttcgtgcgaa gatctactta tataacaagc tttctctgaa atttttttaa
     1201 aatgctattg acttagagtc aactctaagt agtagaatag ataaagtgta acaaatctat
     1261 tttcattaaa agaagatagt acggaggaga gaatgtatta tgagtacagt attacatgta
     1321 ttggatgcaa tcaatagccc cgcagatgtc aaaaaattat ctgttgagga aatgaaacag
     1381 ttggcagaag agatgcgcca aaaaatcttg caaaaggaca gcgatgtagg tgggcacgtt
     1441 ggtccgaatc taggtgtagt agaattaacg attgcgtttc attatgtatt tgattcacca
     1501 aaggataaaa tcgtttggga tgtttcccat cagtcttatg gtcataagct tttaactggt
     1561 cgaaaaaatg gttttgaaaa aggacattac cgtgaagtga gcggatattc caaccaacat
     1621 gaaagtaaac atgactattt tacagtgggg cacacatcta catctatcag cttagcaaca
     1681 ggtttagcta aagcgagaga tctgaagaaa gaatcaggaa atgtggtagc atttatcggt
     1741 gacggctccc taagtggtgg cttagctttt gaaggattga acaatggtgg tgttttacaa
     1801 tccaatctaa tcgtattagt aaacgataat gagatgtcta ttgatgagaa tcatggcagt
     1861 ttatataaaa acttagctga gcttcgtcaa accaacggta aaagcagcaa taacctattt
     1921 aaagcaatgg gatttgatta tcgttacgtg gataatggaa acgatctgga aacggtgatc
     1981 cgtttatttg aagaagtaaa agatattgat catccgattg tacttcacgt ccatacggaa
     2041 aaaggtcgcg gttatcagcc ggcaatcgat aataaaatga aatatcattg gcatgtacca
     2101 tttgatctgt ccaccgggga ggcaaagcaa gtaacttctg gagaaacatt taatatgatc
     2161 atcatggatt atttggataa gaaaatgcaa acaagtacag ctcctattgc cgcagtcaat
     2221 gcagcgatac caggcgcctt tggactgaaa acattccaag aaaaacatcc agatcattat
     2281 tttgacgtgg ggatcgctga acagcactct atcactttcg cttctgcttt ggcttctcag
     2341 ggagtacgtc cagtcgtctt ccaatcaagt actttcttgc aacgggcata tgatcaatta
     2401 tcacatgatt tagcgatcaa cgagaatcca gtgattattt tagtcggtgg cggaacgatc
     2461 tcagctagtg accgaacaca ccaaggaaca tttgacattc cttacttatc aagtatcccg
     2521 aacatcaaat atcttgctcc agcaacaaaa gaggatatga tctctatgct agattgggcg
     2581 ttcgaacaaa cagcgcagcc tgtagcaatc cgaattccta cacatggtgt agaacatggt
     2641 acagctatcg caaatgatta tagtattcca gcctatcaaa cagtaaataa tggaggaaaa
     2701 gtagcaattt tagctttagg cggttttctt gaattaggaa aagaaacagt aaaagctttt
     2761 agaaaaaaag gaatcgaagc aacgttgatc aatccaggaa cgatcacaga aaatgacgta
     2821 gaaacattag ataaattaaa agaaacacac gaattagtca tcacattaga agatggtagt
     2881 ttgtcaggag gatttggcga aaatatcagt cgttattatg gtgattcttc aatgaacgta
     2941 ttaaattttg gtgcagagaa agaattcacg gatagcgttc ctgtagggca gctttatgaa
     3001 cgttatcatt taacaccaaa acaaatcgtt gaagatagtc tgcgtgtctt gaatattcaa
     3061 taattcatta tgctcaccat ccagataatt gtttatacta gtatctggat ggttttttca
     3121 ttataaaaag caaaggggtg gatgttatgt atcagcaagt agaagaatat gtcaaaaagt
     3181 tccaagaaaa atatcctagt tatcaatcag aaaaattagg tttcttttca tttggggata
     3241 cagaaaaaat ggcagacgag ttgagcgaac tagttgtaaa aaagataaag actggtacat
     3301 caagtggtta cgatctgtat aaacaagaga atgagccttt accaaaagtt ggtcaattag
     3361 acgtggtcct gaatggaaaa aatgaaccag tttgtatcat caaaaatata aatgtgacca
     3421 tcttgccttt tagtgaagtc acgaaagagc atgcttggaa agaaggagaa ggggatcgga
     3481 cattagctta ttggcggaaa gtccatgctg agttttttaa acatgcttat aaagaagcgt
     3541 tgaacataga atttacagaa gagaagctgg tcgtatacga agaatttgaa gtaattttct
     3601 tttagtaaga aaggtctgct gaaagaaata gctcattatt gtctcctgaa tagaaaaggg
     3661 ttacattttt atatgaagct gtatttttat ataacattac tggaggagat attgtggata
     3721 cactggaaca aatcaaaggc ggagtcgtcg tttcttgtca agctttggaa aacgagccgc
     3781 tacacagttc ttttattatg gggagaatgg ctttggctgc aaaagaaggc ggagcagttg
     3841 gtattcgagc aaatacgaca gaagatatca atgaaatcaa gcgaatcgtt gatttaccgg
     3901 taatagggat tatcaaaaga gattacgatg ggtcagaagt gtttattact gcgacagaaa
     3961 aagaagtgga agaacttttg gagacgcaag cagaaatcat tgcactggat gcaacttctc
     4021 gtaaacgtcc gacagaaaaa acaactgctg aacttgtcca aatgattcac gaacacggtc
     4081 ggttagctat ggcagatatt tcgacttttg aggaagcgct tcaagcgcaa gaagatggtt
     4141 ttgatctttt gtctacgact ttagcaggct atacagaata ttcacgaaag acgcagacac
     4201 ctgattttga tttattgaat caaatcatag aacaagtcga tatcccagta attatggaag
     4261 gacatactga ctctcctgaa caagtagaga aagcatatga attaggtgct ttttcagtcg
     4321 tagttggatc tattatcacc cgtccgcaat tgatcactaa aagatatgtc gaagccgcaa
     4381 gtaaagcaaa taactaaaaa acacgcacgg cacagattct tgacaaaaaa gaaaaattga
     4441 tagatagttt aaagcagatt ctaagaagat gaaaaaagca ttacttttca ttttgtcttc
     4501 aagatctgct ttttaacgaa ttttatagaa agaaggcgaa accatgtttg aacggagtcc
     4561 ttcagaattg acagaacgtg aaaattacaa gttgctgatt ggttcagtca tccctagacc
     4621 agtagcagta gtaacgacta aatcagcaaa agatatagtg aatatcgcac ccttcagtta
     4681 cttcaatatc gtcagttcga atcgacccat cttatccttg gcgatccaga gaaaacaaga
     4741 tgcagtaaaa gacacagcaa agaatattct tgagacaaga gaagctgtta tccatatcct
     4801 tgatgaggac aacgtagaag aagtaaataa aacagcagcc aatctgccta atgaggaaag
     4861 cgaactgtac ctgactgact tgactttaac tgattcagta atcatttcag taccagcatt
     4921 gacagaagca aaagttcgtt ttgaaacatc cttatatgaa catgtcgaga tacggaataa
     4981 aaataaagta acagctgatt tatttttgct agaagtacag aaatatcatt taaatgaaac
     5041 agtctatgat gataagacag gaaggatcga tccaagaaaa ttgaatgctg taagccgttt
     5101 ggcaggaaat gattatgcag caataggaga aattttcacg attgcacgtc ccgaataaaa
     5161 aggagcacta gttatgttaa aaataagcta taaaccatcg acagattcaa aagaaatgaa
     5221 aaaagaatat gaaacagtga atgattttct gcaagggcag tacttagaag ttccgccatt
     5281 gcaagaccat tttgtcgtta ctactgttac tttagatggt aaggaaatag aaatgccaga
     5341 tcaaac
//
LOCUS       DAJSKS010000154         5312 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJSKS010000154 DAJSKS010000000
VERSION     DAJSKS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.160"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            180..2333
                     /locus_tag="ON396_002495"
     CDS             180..2333
                     /locus_tag="ON396_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HCR3114541.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2302..3717
                     /locus_tag="ON396_002496"
     CDS             2302..3717
                     /locus_tag="ON396_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR3114542.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3750..4784
                     /gene="sppA"
                     /locus_tag="ON396_002497"
     CDS             3750..4784
                     /gene="sppA"
                     /locus_tag="ON396_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR3114543.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4786..5307
                     /locus_tag="ON396_002498"
     CDS             4786..5307
                     /locus_tag="ON396_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR3114544.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
BASE COUNT         1921 a          729 c         1006 g         1656 t
ORIGIN      
        1 tcaagtttat tcttgaatag taaaatttcc aaaataaatt tgattcagga atgaaaaagt
       61 tagtgttcaa gaactgtttt caaagttttt gtggaattat gagacaatat tttcactgta
      121 ttaaagctat attgagaatt tttctataaa aattttttag aaggagttgt gttagataag
      181 tgataaaatc atttcctttt gtgcagcaac aagatgaaaa agattgtggt gttgcttgcc
      241 tttcgatgat tttaaaatat tttaaaactg atatcccgat tcataaatta cgggacctat
      301 ctgggactga tttagagggt acttcagcat ttggattgaa aaagacattt gaaaaattgt
      361 cttttgattg tatggctatt caagcagata gtgagatctg gaaagaaaga gatcttatct
      421 taccattaat tgcacatata ttggttgata acagctatat gcactacgtt gtaatttata
      481 aaacgaaggg aaacgtacta tggattgcag atcccgctaa tggaaaaaat agaaaaacga
      541 ttgaagcgtt tcaaaaagaa tggacaggta ttcttttatt acctatgcct agagaaactt
      601 atattcctat taaagaaaaa gtagctggat taagttcttt tttgcctata atttggaaac
      661 aaaaaggatt agtattccac atcgttttag catcgatttt cattacgttt tttggcatag
      721 gaagctcata ttattttcaa ggtattcttg attttttcat ccctaatcaa gcacgttcaa
      781 ctttaaatat aatttccatt ggcttgatcg ctgtctattt atttcgggta ttgtttgaat
      841 atagtcgtag ttatcttctt attattttag gacagcgaat gagtatggca attatgcttc
      901 agtatttcaa acatgtgtta actttaccaa tgaatttttt tgctacgaga aaatcagggg
      961 aaattatttc tcgttttctt gatgccagta agattattga tgctttagca agtgctacgt
     1021 tatcaatatt tttagatatt ggtatggtgt tgcttgttgg ggttacgtta gctattcaga
     1081 atagtacgtt gtttttaatc actctatgct ctttgccttt ttatgtagtt gcaatattcg
     1141 ctttcgtgaa aagctatgaa aaatcgaaca gggaagaaat ggcagccggt gcagctgtaa
     1201 attctagtat catagaaagt cttaaaggaa tagaaacaat taaagcatat agcggtgaag
     1261 agaaagtata taaccgtata gaccatgaat ttgttaaatt aatgaaaaaa tcatttcgta
     1321 cggttacgct tgataatatt caacaaggaa ttaaacattt gattcaatta attagcagtg
     1381 cattaatttt atggctaggt tcttattttg ttatggataa tagaattagt ctaggtcaac
     1441 ttatcacata caatgcttta ctagtatttt tgacagatcc tttacaaaac attattaatt
     1501 tacaagtgaa aatgcaaacg gctcaagttg caaataatcg tttaaatgaa attttcgcga
     1561 ttgattcaga aaaatcgaac cagtcttttg aatatatgct ttctgaacaa ttatttcaac
     1621 aagatatcac tgtaaaaaac gtttcattct cgtatagcat gaaggcccca acgttgaacg
     1681 aaatttcatg tacaattcct tcgcacagta aaacggctat agttggagtt agtggttctg
     1741 gaaaatcaac tcttgcaaag ttatttgttc gtttttatga agcatcagaa ggatcgatcc
     1801 attatggaaa cattaatcac ttagacattc cttttcagat attacgcgat cgagtgacat
     1861 atttgcctca agagtcattt ttctttagtg gaacaatcat tgagaatctt atattcggtt
     1921 tagatagccc accaagtttc gaacaaataa tagaagtttg tgagatagtc caactaaatg
     1981 aatttattaa tcaacagcca ttacgttacg agacattgtt ggaagaaggt ggaaataatc
     2041 tttcaggagg gcaacgacaa cgattagcct tagcacgggc tttattaaaa aatccggaca
     2101 tattaatttt agatgaagca actagtggat tagatacctt gttagaacat gcaattatga
     2161 attatctcct caatttaaaa gaaaaaacga ttatttttat tgcgcatcat ttaccaatag
     2221 caaaagcatg tgatcaaatt ttagttctcc atgaagggca tttggtagaa aaaggaacgc
     2281 acgatgaact tcgttttgcc gatggtatct atcaaaaatt atgggaaatc taggagaggg
     2341 aaacaattta tgcagaataa taattggttc gaacattcta gtatctatac taaacaacat
     2401 catagcttct accgttgggt gttataccct gtaattcttt ttttatgtat tattggattg
     2461 tttttaacct ttgctaaaaa agaggttgtt attcggacaa cggctcaatt gaaccccgaa
     2521 aaaatagaga agttacaagt gccattagaa gctaaaataa tggaaaactt cctaaaagaa
     2581 aatcaatttg ttcataagga cgatgtgttg gttagattgg attgttcttt aatagagaat
     2641 gaaaaggctc agatagaaca agaaaaccaa cgtattactc aacaaataaa gatggctcag
     2701 ctatttattg aaagtataag taaaggaaaa aatttgtttt caacggatga cagttttggc
     2761 tacagtaatc aattaaagag catgttgtca gaaaaagaat cactccgcta cgctttgaag
     2821 caaagtgaat taaatgatca aaagcaatta gaagtatacg aaaagacaaa aagacaacta
     2881 gaaaaacaaa ttgagagttc agatagtaaa ttacaagaat ggcaacaagt acaggtagct
     2941 tggagtaata atcaatcatt aaaagatttt tcaaaagaaa tgatggcaaa ctatgagaat
     3001 tggcaagaac aactaaataa tgtttctgat gatcaaaaaa atcaagtgaa actgacaatt
     3061 tcagcaagca taaatgaaca aattgagcaa ctaaaaaaag aagtagaaca gtatcagtca
     3121 gaaaaagcta aattagttaa accaactact tctgagaatg acagaattag tcaaacggaa
     3181 aaaggaaagc aagagctaga acaagcaata acaactgtaa aacaaacagt tacagagcta
     3241 aatgaaaaac aagagaagaa ccagtcagtt atcaaaagtt tggacgatca actttcaaaa
     3301 ggaatactaa aagctcctgt atctggaacg gttcatttaa atgaagaaac aaagggacaa
     3361 gcagaactag ccaaaggaac agtgcttgca gaaatttatc cgatccacga aaaatcaaaa
     3421 atgaaattta cagcactttt acctgcgaat gaaagtacct atattaaaga agggatgaag
     3481 gttcatttta aattggatca aaaaggtggg gctccgatta cgatcgacgg aactttagat
     3541 gaaatttcag aaaattctac tgccactgag aaaggtgttt tttatgcagt gaaaggaacg
     3601 ttaaaaacaa ctcaatcgtt tccgtttcgt tatggattaa ctggagagtt gtctttaatt
     3661 gtaggagaaa aaacatattg gcaacaaatt gtcgatgtgt ttctaaataa aaaataatta
     3721 tttttactaa gaatatagga ggagtttata tgaataaaag aagatggatt gctgtgggag
     3781 tagcgtgcgg attattgtta ctatccatta tcgtcgcgct aattccagga aaagataaag
     3841 aggaagcatc aaatacaacc ctaacaggaa taaataagat tttttacgga tctaacgaaa
     3901 tcacagaaga aacgttagaa gaaggcgcat caaataaaaa gattgtcaaa ctatccgtca
     3961 atggagttat tgcagataca ggtgaaagca atctttttag tagggaacaa tataaccatc
     4021 agaatttttt aacacagcta aagaaaattc aagaagacaa agcggttaag ggagtgttat
     4081 tagaagtaaa ttcacctggg ggaggaatat atgaaagtgc tgagattgct aaagaaatgg
     4141 caaaaattaa aaagctagat attcctatct atactgcatt taaaaataca gcggcaagtg
     4201 gagggtacta catttctgct ggttcagata aaatatttgc tacggaagaa acaacaactg
     4261 gatcaattgg tgtcattatt tctggtttaa attattctgg cttattagaa aaacttggcg
     4321 taactgatgc gacgtataaa agtggtgcat taaaagatat gatgtcacct caacacaaac
     4381 cttcagagga agagaataaa gttatacagg aatttgtgat gagtgcttat gatcgttttg
     4441 tcaatgttgt agcaaaagga agaaatatgg atactaatgc agtgaaagaa cttgcggatg
     4501 gaagaattta tgatggtaac caagcagtag aaaatgggtt ggtggatcaa attggttact
     4561 ctgaagatgc attagattcc ctaaaaaaag agaagaaatt aactgatgcg acggttatag
     4621 aatataaaaa tgacacaact ggctttgctt cttcttggtt aggtaataag attgctgaat
     4681 ggcaagggct aaaggcaact acatcagatc gtgtaatttc agtttttgaa aaacttggat
     4741 tttctgatac tccaaaacca atgtattact atggagggct ataaaatgaa taagaagagt
     4801 ggcattgaaa ctttgcctaa ttatgtattt gcgggatttt ggttacgatt ttttgctttt
     4861 cttattgatt taatttgtat tagcatcatc acccatgcta ctataggtct cttttacaaa
     4921 atcatgggac tggaatctga caaatccatt attacttttt atggtatagc gtcattagct
     4981 atttatttaa cctattttgt acttttgact aagctgaatt caggacaaac aattgggaaa
     5041 atgatttttg ggataagagt agtgagcttt gaggaaaaag agctaagttg gcaaactatc
     5101 tttattcgtg agtgtatatg taggtttata ttaaaaaatt ttgtatttca aattgggtat
     5161 ttggtggctg ctttcacgcc caagaaacaa catattgggg attatttttc taacacgtca
     5221 gtggttgtta ttcgttcatt agaagctatt aactatggtc aaaaaatttc taattctaaa
     5281 acacttaatc tggagagaat taaatgaata ag
//
LOCUS       DAJSKS010000155         5311 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJSKS010000155 DAJSKS010000000
VERSION     DAJSKS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.161"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            22..879
                     /locus_tag="ON396_002499"
     CDS             22..879
                     /locus_tag="ON396_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR3114545.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            994..1914
                     /gene="pstC"
                     /locus_tag="ON396_002500"
     CDS             994..1914
                     /gene="pstC"
                     /locus_tag="ON396_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCR3114546.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1914..2798
                     /gene="pstA"
                     /locus_tag="ON396_002501"
     CDS             1914..2798
                     /gene="pstA"
                     /locus_tag="ON396_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR3114547.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2813..3619
                     /gene="pstB"
                     /locus_tag="ON396_002502"
     CDS             2813..3619
                     /gene="pstB"
                     /locus_tag="ON396_002502"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCR3114548.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3635..4393
                     /locus_tag="ON396_002503"
     CDS             3635..4393
                     /locus_tag="ON396_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCR3114549.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4406..5083
                     /gene="phoU"
                     /locus_tag="ON396_002504"
     CDS             4406..5083
                     /gene="phoU"
                     /locus_tag="ON396_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR3114550.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
BASE COUNT         1669 a          949 c         1190 g         1503 t
ORIGIN      
        1 taggattaaa ggagatttct aatgaaaaaa cttaaattat tcttaccaat aataggatta
       61 gccattcttt tatcaggctg ttcaaagtgg attgatcgag gagaatccat tacggctgtt
      121 ggctcttctg ctttacagcc tttagtagaa acagttgctg aaacttacca gacagaaaat
      181 cctggaaaat tcatcaatgt acaagggggc ggaagcggaa ctggtttgtc tcaagtacag
      241 tcaggagcag tcgacatcgg aaactctgat ttgtttgcgg aagaaaaatc ggggatcgat
      301 gcctcagcat tagtcgatca tcgagtagca gttgttggga tcactcctat tgtgaacaag
      361 gatattggcg taaaagacat cagtatggaa aacctgaaaa aactatttac aggcgagatc
      421 aaaaactgga gtgaagtcgg tggaaaagat cagccagtag ttatattgaa ccgcgcttca
      481 ggaagcggaa cccgtagcac gtttgaaaaa tgggtattgg acggagaaac agctgttcag
      541 gcacaagaac aagattcaag cggaatggtg cgccagatcg ttgccgacac acccggtgca
      601 atcagctatg ttgctttttc ttatgtttcc gatgatgtag atacgttgaa tgttaacggt
      661 gtagaaccga cagatgaaaa tgtgacaacg aacaaatgga cgatttgggc atatgagcat
      721 atgtacacaa gagatcatcc aaaaaaactg acaaaagaat ttttagacta tatcttatct
      781 gatgaagtac aagataacat cgttggagaa ctaggttata tccctgtttc tcatatgaaa
      841 gtcgaacgtg attggcaagg aaatatcgtt aaagagtaat ctttacacaa aatttacaaa
      901 cttagctaaa cttttacatg ttattaaaac caaattgatg ttcaatttgt acactgagtg
      961 agtatgttaa acctgattaa gggggagtca tctgtggaag atgtgaaaaa agtattatta
     1021 acaaaatcaa aacgttcaaa aatggaacag cgtggaaaat tcatcagttt tttatgtatc
     1081 gcgctgatcg tgttggttgt attatctatt ttttattttg tagctagtaa aggattggcc
     1141 acctttttta ctgataagat caatgtattt gattttctgt ttggtacaga ttggaatcca
     1201 agccaaatcg gagcaaatgg gaaaccaatg gtaggggcat tgcctatgat cacaggttcg
     1261 tttattgtga cctttttatc tgctatcgtt gccacgccat ttgccattgg tgcagcagta
     1321 ttcatgacgg agatctctcc taaaaacgga acgaagatct tacagccggt catcgagctg
     1381 cttgtaggta ttccttctgt cgtttatggt tttatcggtt tatcagttat cgtgcctttt
     1441 gtcagaagta tttttggcgg gacagggttt ggtatcttag ctggtacatt tgtgttgttc
     1501 gttatgatct tgccgactgt cacaacgatg actgttgatg cattaaaggc agtaccacgg
     1561 cactatcggg aagcttcatt agcgcttggt gccacaagat ggcaaacaat ctacaaagtc
     1621 gtgctacgag cagccgtgcc cgggatttta acagcagtcg tttttggtat ggctcgtgcg
     1681 tttggtgaag ccttagctat ccaaatggtc atcggtaatg cagcattgat gccaacagga
     1741 cttactactc ctgcttctac attgacgtcc atcttaacaa tgggaatcgg aaatacaatc
     1801 atgggaacag ttgaaaataa tgtgttatgg tcattagcgc tgatcttgct attgatgtca
     1861 ttattcttta atatcgtgat ccgtatgatt gggaagaaag gagagctgaa ataatgaacg
     1921 caaaacgctc agataaaatt gctacaggtg ttttatatat agtttcgggg atcatcgtat
     1981 tgattttagc agctttacta ctttatatct tagttcgggg aataccgcac atttcatggt
     2041 ccttcttgac cgaaccttcc aaagcctacc aagaaggtgg tggaatcggg atccagctat
     2101 ttaactcgat ttatttgctt ttgatcacga tgatcatcag ttttcctatt tctttaggtg
     2161 cgggtatcta tctttcagaa tatgctggaa aaaactggct aacagatgtg attcgcacgt
     2221 cgattgaaat cttgagctcg ttgccttctg tcgttgtcgg tttattcggt ttcttggttt
     2281 tcgttattca attccagtat ggtttctcga ttatttctgg tgcattggct ttgactgtat
     2341 tcaacttgcc tcttttgact agaacagttg aggagtcttt acaagcggtc cattacacac
     2401 aacgagaagc aggattggcc ttaggtttgt cacgctggga aacagtgatg aaagtggtgg
     2461 ttccagaagc gacaccaggg attttgactg gtgtgatcct aagttctgga agaatttttg
     2521 gtgaagcagc tgcattgatc tatacggctg gacaaagtgc acctgcgtta gattttacga
     2581 actggaatcc attaagtgtt tcaagcccga tcagcatttt ccgtcaagct gaaacattag
     2641 ctgtccatat ttggaagatc aatacagaag gcacgatgcc tgatggtgtt tctgtttcgg
     2701 ctggcgcttc tgccgtgttg atcattgctg ttttactgtt caacttcggc gctcgtgcga
     2761 ttggcaaacg attataccga aaaatgactt cagcctaagg agaagaagca aaatgaaaaa
     2821 atataactgg aatgaaacaa atgtcatcac catgccaaaa gagaaagaag tagcactata
     2881 tacggatgat atacatgtct ggtatggcga caatgaagca atcaaaggag tggatctgga
     2941 atttgaaaaa aataagatca ctgcactgat tggtccttcc ggctgcggaa aatctacgta
     3001 tcttcgttcg ttgaatcgaa tgaatgatgg gatcgctaat acgaaaatca ctgggaaaat
     3061 catgtatcaa ggcgtagata ttaatgcgcc tcaagtggat gtttatgaga tgcgcaaacg
     3121 gattggcatg gtttttcaac gtcctaatcc gttcagcaag tcgatttatg acaatattac
     3181 atttgctttg aaacagcacg gcgaaaaaaa caaaaagaaa ttagatgaaa tcgttgaaac
     3241 aagcttgaaa caagctgcct tgtgggacca agtaaaagac agcttggaca aaagtgcctt
     3301 ggctttatct ggcgggcaac agcaacgttt atgcattgct cgtgcgatcg cgatgaaacc
     3361 tgatatcttg ctgctagacg agccggcaag tgctttggac cctatttcga caggaacggt
     3421 tgaagaaaca cttgtaaact tgaaagaaga ctatacgatc atcatcgtga cgcataacat
     3481 gcaacaagca gctcgtatca gcgactatac tgcctttttc tatctaggaa aagtaatgga
     3541 atatgacaaa actaacaaga tatttaccag accaaaaatt caggcaaccg aagattatgt
     3601 gtccggtcat tttggttaag acggaggcct caacatgaca aaagaaatta tttcatctaa
     3661 agatcttcat ctgtattatg gaaagaaaga agcactaaaa gggatcgatc taagtttcaa
     3721 tcaaggggaa atcactgcga tgatcggtcc ttctggttgc ggaaaatcca cttatctgcg
     3781 ttctttgaac cggatgaatg acctgattcc gaatgtaacg atcaccggaa gtgttttata
     3841 taaagacaaa gacatctatg gtccaaaaac agatgttgtt gaactaagaa aagaaatcgg
     3901 catggtattc cagcagccaa acccatttcc tttttctatc tatgaaaatg tcatctatgg
     3961 attgaaatta aaaggggaaa aagataaaaa agtccttgat caagtagttg aagaaagcct
     4021 gaaggcagcg tctgtttggg atgatgtgaa agacaaattg cataaaagtg ctttatcttt
     4081 atccggcggg cagcagcagc gtgtatgtat cgcacgtgtt ttggcagtta atccggaaat
     4141 catcttattg gatgaaccaa caagtgcgct tgatcctgtt tctactggga aaatcgaaag
     4201 tatgctgtta gaattaagag agcggtatac gatgatcatc gtgacccata atatgtctca
     4261 agcttcacgt atttcagata agacggcatt ctttttagat ggtcacttga tcgaatacaa
     4321 cgatacgaag aagatattca tgaatccaga aaaacaagag acagaagatt atatttcagg
     4381 tcgatttgga taaggaggaa acggaatgct acgtacacaa tttgaagaag aattgctgaa
     4441 tctccataat cagttctacg aaatggggat gatggtgagc agcgccgtgc acaagtcagt
     4501 tcgtgcatac atcaaacacg ataaaatctt agcccaagaa gttatcgaaa acgatataaa
     4561 tatcaataac atggaaacac gcttggagaa gaaaagtttt gaaatgatcg cattgcaaca
     4621 gccagttaca actgatttgc gtatgatcat tacggtcatg aaagctagct cggatttaga
     4681 acgtatggcc gatcatgcgg tgtctgttgc taaatccacg attcgcgtaa aagggcagac
     4741 gagaataccg gaaatcgaaa aagagatctc cgatatgtcc gattatgtga aaaaaatggt
     4801 ggacaacgtc ctagttgctt atgtcaaaac agacgaaaaa gacgcacgta cgatcgccaa
     4861 tatggatcaa agagtcaatg actatttcaa tcgaatctat aatgcaacaa tcaaaaatat
     4921 gcaggaaaat cctgagacag tcatcagtgg aacagactac ttgaatgttg ccagttatct
     4981 agaacggatc ggagactatg tgacaaacat ctgtgaatgg atcgtctact tggctacagg
     5041 gaaaatcact gagttaaata cgaaccacaa tgagattttc taaactgaag ctgtgattat
     5101 gccgtttgtt tatgaaaaga ggttgtgaaa aagctgttct acatcaagta ataagaacag
     5161 tcaaacaaaa aatagctcct catatttttc gtttggctgg acttattacc gcagatgcaa
     5221 gcttttgaac accgtttatt cgggaaaggg tttgtgacaa aacatgtcac aaaccctttt
     5281 catcatttca tagaaaggat aaagaatgca a
//
LOCUS       DAJSKS010000156         5291 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJSKS010000156 DAJSKS010000000
VERSION     DAJSKS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.162"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ON396_002505"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ON396_002505"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="ON396_002506"
     CDS             complement(580..1353)
                     /locus_tag="ON396_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3114551.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="ON396_002507"
     CDS             1497..1847
                     /locus_tag="ON396_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HCR3114552.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="ON396_002508"
     CDS             1828..2544
                     /locus_tag="ON396_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3114553.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="ON396_002509"
     CDS             2544..3992
                     /locus_tag="ON396_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3114554.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="ON396_002510"
     CDS             complement(4062..4571)
                     /locus_tag="ON396_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114555.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="ON396_002511"
     CDS             complement(4561..5286)
                     /locus_tag="ON396_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114556.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1787 a          929 c         1062 g         1513 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt t
//
LOCUS       DAJSKS010000157         5281 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJSKS010000157 DAJSKS010000000
VERSION     DAJSKS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.163"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(3..278)
                     /locus_tag="ON396_002512"
     CDS             complement(3..278)
                     /locus_tag="ON396_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HCR3114557.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(250..1203)
                     /locus_tag="ON396_002513"
     CDS             complement(250..1203)
                     /locus_tag="ON396_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3114558.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1815..3308
                     /locus_tag="ON396_002514"
     CDS             1815..3308
                     /locus_tag="ON396_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HCR3114559.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3443..3739
                     /locus_tag="ON396_002515"
     CDS             3443..3739
                     /locus_tag="ON396_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCR3114560.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3840..4691
                     /locus_tag="ON396_002516"
     CDS             3840..4691
                     /locus_tag="ON396_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114561.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            4712..4995
                     /locus_tag="ON396_002517"
                     /pseudo
     CDS             4712..4995
                     /locus_tag="ON396_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1802 a          821 c          998 g         1660 t
ORIGIN      
        1 ttttaatctt ttgatcgtct attatatata atccgttcct cgtcgtttaa agtatcaaca
       61 taggcgtcca ttgcaagttt gactatttca tacataggaa tatcttttgc ataactaaga
      121 tcccgaatgg tttttagtac gggaggatct acttgaactg gttttctttc tttcgcagtg
      181 tactttcgtt cccctaactc gacattaata tcatctttaa ttgaaatatc ttcagcacca
      241 attttcttct tatctttgtt cgttggtaat aatgccattt agtttaatct cctttccaag
      301 aggtaggata tgattggcca ttgagtcagc atatttaggt gtataatcaa atccttcaat
      361 atcaccatgt tgttcatagt attcaattct ttcttctaat tctgtaaaga tgtctgcaaa
      421 aatagcccac catcttcgat ctacataatc atttaattgg ataccattct caccatacat
      481 ttctaaccga tccgagcctt taacaatgct tttaaaaatg tttgattcac catacttatc
      541 aattgttttt tgatagtttg cgtgttgaga ggaacgtcgc actgagaaaa gaacaggcaa
      601 aattccaatg atttgaagat gagactcttc aaaattcgtg acaatcggaa ctaaaacttt
      661 attaataaaa tcttctgttc cttccattgc gtaccgtttt aattcttgca ttgcaataat
      721 ataatctgca gcagttaaga atgcacgaac cacattatct gttgatggtg ggcagtcgat
      781 aaagatataa tcgaaatttt ttctcaattc ttctaaaggt ttaatgaagg ccaatgatct
      841 cttacgacga tcaggatatt tttcggacaa gtattcatta agttcttcag atccaggaga
      901 gccagccata atatataaat gtggacttac ttgagtaata cctgaaaccc agtccccttt
      961 tttgattgct gcagacatgg atcgatcaaa gctagtaatt tgatacgttg atccaatgtt
     1021 ggaagtagta tttctttgtt ggtcataatc aattagcaac acgtcttttt caaaaagttg
     1081 actagaaaca atcgctaata aatcattgtc tgtagtctta ccaatgccgc cttttctacc
     1141 tgtaagcatt acaacttttc ctagtttatc tgatgtttta aatgcagtcc gtaagttttc
     1201 catagttccc tccaatttcc tatactaaat atatttagta tacttcatat acttaatata
     1261 ttcgatatat ctaatatact ccaaattgat aagaattcag actttataag gaatatagtg
     1321 gatatactaa atatatttgc tatattcagt atatcagata tattcaaaat gtcaaaatat
     1381 atttgtgcgt tttgcgttga caatttagag tgaaaagagg taaacttaat tctatagaaa
     1441 ccacagataa ttcactattt acaaagtagc aaaagcagtg gtactcttta aacagaaaaa
     1501 ttacataaaa aaaagagacg gaaataattg attttagcag aatcaattat ttctgaatca
     1561 catggttagg acatgtgttc acgctcgtct caagaatatg taagtagcta ccaactactt
     1621 atattaaaag ttgatcggat accttttccg attgccttta ttctaccaaa aaaaggcgag
     1681 caactcaaca gaagaacgtt tatttagtgt acttaaatta aatattctta aacactgaag
     1741 agagaacaca tactatggct gagattaaag ctaaactgtg ttctcttttt gtttgagaaa
     1801 ggacgttaca atcgatgttg agcgaaattt attcagctat tcttaaaaac ggcttacgta
     1861 catacaaata tcgaaacagc accatgaaac ctgcaggtca tattgattcc agtaggagtg
     1921 gtgctatctt tgcgtaccgt agcaagcaat taatgaatgt tgggcgtggt atggtcatta
     1981 cttcggaaga agctatttta gagaacgaaa agaaactaac gcattggaca cctaatacct
     2041 atcgctttgg tacttatgcg gatgattatc gaacagtggt taaaggtcat tctgagaaaa
     2101 acttaagtca aatcaacaca tttgtagttg atatcgattc aaaagagaac catcaaggcg
     2161 aaattatttt agcctgttta gatcaagtgg gctacatgcc aacgctaatt ttagaatcag
     2221 atcatggtta tcaagtttat tttgtactaa agacaccagg ctatgtcaca aagaaaagca
     2281 atttcaaggt gatcgatgta gcaaaaaaaa tttcaaaaac aattcgtcta cagttggctg
     2341 aaaaattgtc cggagtggat ctaggatgta accacttcgg aattgctcga ttcccacaag
     2401 aaaataatat tgtttttaaa gaaatgaact atcagtattc ttttgctgac tggattcaat
     2461 ggtcgatgaa gcttgaatca gatcaaaaaa caaaagaaga tcgagaacga aaactaattg
     2521 tctttcctga gaaaaaagaa attcgtcaag tagatgaacc gtggttcgat atgttacttc
     2581 ataaagcaaa catcgtcggc ggagaaggac gtcttggtcg aaataatgtg attttcacgc
     2641 tttctttagc ttattactct tcaggttatg cattggatac ttgcgaatac aatatgttca
     2701 cattcaacga acggttacag gaaccgctat ctgaagcaga attagtaaag attgtacgta
     2761 gtgcttattc tggtaattat caagcagcaa gcagagaatt cattattgaa ttgtgccagc
     2821 aatgggtttc accaacctta aaaaataatg atctgttcat tcaacgacgt ggctggtgga
     2881 aatttaaaaa gccacgagat caaagaaaat atagtcataa aaatgaatgg aaagaagatc
     2941 tattggctta tctaagcgaa aaaagtcttg ctagtgagcc ttatttgaac ctgtcaaaaa
     3001 aagaattggc ttcacaacta acgatgccca agcgaacact tgataagatc ttaaatgagt
     3061 tacgagagga gaataaagtc ttctacaagg taacacgagg aagaaatggc ggaatgatca
     3121 ttgcttcagt caaaacgttg ttcgcgaggg ttattcaact aagtaaagaa aagagactag
     3181 attattcaac aaggatcaca gaggtatttg gactcaacga gagcttagta gaagagacat
     3241 ttagacaatt atcaaccccc ttgaaccctc ttaaagaagt gaatcttttt gaagtagata
     3301 caggttgaaa aataaaaggt gcaaattgcc ctatacaata tatctatgat acttctgtag
     3361 gtgttgctcc ctttgcctag ctacgtgtag gtggtatact tataactgcg tatgcagtct
     3421 tatgtatcat ggaggaagtt gtatgtcgtt aaaaaattat gtctattcac atcctgttaa
     3481 tgtgtttatc attggtcgtt tgggattatc tgtggaagtg ttctgtgagc tttatggttt
     3541 taagcagggg actttgtctt cttgggttac cagggaaaaa acagttgctt ctttgccgat
     3601 tgagtttctt catgggttgt ccttagcttc tggggagact atggatcagg tatatgattg
     3661 tttgtgtgtg ttagagcagg agtatattga gtataaaata gctaacgagt taagaaaaag
     3721 gaagaagtat atccagtagt agtttttatt tacttaaaaa attggttcat tgagcaacct
     3781 atcgctgcta aagtagcaaa ggaaaacgtt aaaggaaaga atttagggaa gaaatcgaaa
     3841 tgaaatcgga cgactggatt gaacacacct ggcttcctct gaaaagaaat agcaaacgaa
     3901 agaaattttg ggtattgcaa ttatggccat taggaaaact ttgtagttac ggggcacgac
     3961 ttattcttga tttaaccgtt tactcgattg gagtatttgg aacggcttat tacttgataa
     4021 atgatcccaa taggattgca gcatcagaca tttttacagt gctaatgacg ttgatttttg
     4081 tcatgctatt gtctattttt actcaattag tcatgcaatt taccaggcaa aaattaacag
     4141 caggaaaact tttcaaaagc ctattcgatt taattgttgt aaatgcaatt ctagcgactt
     4201 taataaaaca aattgaacaa tctggaagaa caacaaaagg agtgaaggat tttttattga
     4261 cgtttattca ctttgtaaat ggacacatgt atttgtttga agtgggtatt attctctttt
     4321 ttgtcgggta tgtatccacg aaacttatta aacgttcaat tgaaaaagaa attgaaattg
     4381 acctgattcc gacacttctt ggttatgagt acaatgttga ttttggaaga tatgacgaat
     4441 tgatcaatac ggatatcgtt cacacgagta tccatgaatc tactgttaag ggcatcagat
     4501 ctattttaaa aaatggccag gaaatgaaag caatagatcg aagaaaagag atctacaatt
     4561 tttatgaagt cgaagcaagc ctacgatatg gcatcggaaa attgtggtta gatggaagta
     4621 atattcgtca aaacaaacca tacatttttt ggaacagtag tgatttagaa cttccaatga
     4681 aagataaata attatggaga atcgtttgaa aatgattcct ctatacagga ttgtgcttat
     4741 agttggcatg gagttaaaaa ccttaaagaa ggagcagtta ttcaaagtga aagaacgtca
     4801 taatcagtta ctatgtagta aaacgggtac gatgaacatt gaataccgaa cagaatattt
     4861 agaattaacg aatcaaactg attttttgtt gattcgaaag caacaagagc aagaaaaaaa
     4921 ttaatggcta cttatagcca attgattgat gaatgggaac aattcaagga atctaaaatt
     4981 ccagaacgtc tctaaaagcc tttatttgta aagagcataa ggaaacgtgg gaacactttt
     5041 taaaaaaagt gttccaatag gctgtgccta ttacgtttct caaaaataaa tgtgggaaca
     5101 aaatatttaa aaataggata gtgaaaggat agtgaaggat agtaaaagga gggtagtagg
     5161 atagcaaaaa ggataggctc cttttcgcta tcctttttaa attggaggaa ttaatatgga
     5221 agtaagaata agaaatgtgg atgagaaaat cgtaagagaa attgatcgaa gatgtgacga
     5281 a
//
LOCUS       DAJSKS010000158         5204 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJSKS010000158 DAJSKS010000000
VERSION     DAJSKS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.164"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            16..1134
                     /locus_tag="ON396_002518"
     CDS             16..1134
                     /locus_tag="ON396_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3114562.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1262..2638
                     /locus_tag="ON396_002519"
     CDS             1262..2638
                     /locus_tag="ON396_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR3114563.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3039..4619
                     /locus_tag="ON396_002520"
     CDS             3039..4619
                     /locus_tag="ON396_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR3114564.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            4817..5041
                     /locus_tag="ON396_002521"
     CDS             4817..5041
                     /locus_tag="ON396_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HCR3114565.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT         1749 a          845 c         1083 g         1527 t
ORIGIN      
        1 aaaggaggaa atctattgtt tgaaaaactt agacagtcta aactaatgtt ttggtcgctt
       61 gaattattga tcatagctgc attgatcatg atttcatcag aaataaactt tattttcgaa
      121 ccgatcggga catttttttc cacgttattt gcacctgtac tgattgcagg ctttctttac
      181 tatttattga atcctattgt caagctgttg atgaaagcaa aaatcaaaag aatatgggct
      241 gtcgcaattg tgctgctcct tttagttgca gcaattattt ggattttact tagtgtcatc
      301 ccaagtctag tacagcaaat ttcctcattg gcatctaata tgcctgattt catcaaacag
      361 gtagaaagct ggttaaaaga ggtagccgaa ttgcctttgt tcaaggaagt agatatcaat
      421 aaatattttg agcaattaga catttcttac gggacaatca ttcagcaatt ccttagtgga
      481 gtatccaata gtttaggttc gatcgttgga acgatcgctt cggcaaccgt agtaatcatt
      541 acagcaccat tcatcttgtt ctatatgttg aaagacggag aaaaactagt gccaaacatc
      601 caacagtttt tcccagaaaa aagaagaaac caaattgtgg agttattggg gcaactgaat
      661 aaaacattgg ctaattacat tagcggacaa gcaatcgaat gtttattcgt tggtaccttc
      721 acatttttag gatatatggt aattggtgtg gattatgctt tcttatttgg ggtcattgcg
      781 ggattgacaa acctaattcc atatttagga ccttatctag gactagcacc tgcatttcta
      841 gtaacagtct tcaatgatcc ggtaaaagca ttactttgtt gtgtggttgt gctaatcgtc
      901 caacagttgg atgggaatat catttatcct aatgtaattg ggaaatcact gaaaatccat
      961 ccattaacga tcattatcgt tcttctagta gctggaaata ttgccggatt gcttggtatc
     1021 ttcttaggtg ttccttttta cgcaatctgc aagacgatca tttcctatat aatcaaaatc
     1081 gtcaaagaag acaagcaaaa tgaaaacaag aagaaaattg ctacgacctc gtaggtagtt
     1141 ttagtacact tgtttttatt gcaaagaaaa ttgctttgtg ataccatttt aacgtggcta
     1201 tagtgccacg tttttttcta tcgtaaacta aaaattaatt ttataaggag agaatgcgat
     1261 tatgaatgct gaccctgaga gtcagtcgct gttagcgcag attttattat tgatcgtttt
     1321 gacattgatc aatgcttttt tagcagcttc cgaaattgct gttgtttcga tcaataagaa
     1381 tcgaatcgag caaaaagctg atgaaggaga tgtgaaatct caaaagcttt taaaaatatt
     1441 gcagaatcca aataatttcc tatcgacaat ccaagtagga atcactttgg tcaacatttt
     1501 gtctggtgct tcactagcta atactttatc ggaacggtta gctccggtat taggtggcgg
     1561 cacagcggca aaaaatattg caaatatcat tgtgctggct attttgacct atgtatcgat
     1621 tgtttttgga gaattgtacc ctaaacgtat tgctttaaat aaatcagaag aagtcgcaaa
     1681 gttcacttca gggctgatac gcttgattgg tgtagtagct aagcccttcg tctggctgct
     1741 ttctgcttcc acaagtttat tggcgcgtat cacgccgatg acttttgatg atgaagattc
     1801 aaaaatgacc agagatgaaa tgcgttatat gcttgaaaac gaaggcgtat tgaataatga
     1861 agaattagaa atgctccaag gggttttttc tcttgatacg aaagttgccc gagaagtcat
     1921 ggtcccacga acggatgcat ttatgattga tatcaacgac actgtggaag aaaatgtgaa
     1981 tgaagtttta tccgaaaact attcaagaat ccctgtttat aacgaagaca aagataaagt
     2041 agtcggaatc cttcatacaa agaatttact aaaagctgcc cataaattcg gctttgataa
     2101 tttagatatc aagaaaatca tgcaggagcc tttattcgtt cctgaaacgg tctttattga
     2161 tgatctgtta tatgaaatga aaaagacgca gaatcagatg gctattttat tagatgaata
     2221 tggcggcgtc gttgggttag caacactaga agacctttta gaagaaatcg tcggagaaat
     2281 cgatgatgaa tcagatgaag ttgagaatct ttatgaaaaa atcgatgaac acgaatatat
     2341 catccaagga cgtatgctga ttgatgagtt caatgaagct tttgacagtg atctgcatat
     2401 gagtgatgta gatacgatgg ctggttattt gattacagca ttaggcatga ttccggatga
     2461 aggcgaaaag ttatcgtttg atgtcgataa tattacgctg gtttctgaag aaatggaagg
     2521 ttctcgaatc cttaaaatcc gtgtcatttt tcatgatccg gaagaaacag aagcggaacc
     2581 tgaagaagaa cgaagatact tcagaaaaga attcgaagat gacgagccaa gaagataatt
     2641 ggaaacttgg ttatgaaaga cgtgttgctt gaataaacaa gcaacacgtc ttttctttta
     2701 gtcagctgac aaaaaaatat ttgactctta tggtttggtg gctttttcat tcgctgaatc
     2761 catgctatac tgattcagtt gtagtagttg acaaaggttg tgaaaaagct atcctgcatt
     2821 aagtaataag agcagccaag caaaaacagc ttttcgtatt tttggcttgg gtgggcttat
     2881 tgctgatagg ctgtctctag aacgccgttt actcggaaaa agagactgct acacggcttt
     2941 tgtcacagcc ctatatgaaa ttgtctttct ttaagagatc ggacaatctg tgcctagaga
     3001 aaatataaat aattgaatgg aagaaatgga gttaggttat gaataatcca aaattaaagc
     3061 aacaagtgga caatcgcaga acttttgcga tcatttccca tccggatgct gggaaaacaa
     3121 cgattacaga acaattacta ttgtttggtg gggctatccg tcaagccggg accgtaaaag
     3181 gcaaaaaaac aggtaatttt gccaaatcag actggatgga aatcgagaaa caacgtggga
     3241 tttctgttac tagttctgtt atgcagtttg attatcaagg gaaacgagta aatatcttgg
     3301 atacaccagg acatgaagat ttttctgaag atacttatcg tactttgatg gccgtggata
     3361 gtgcggtcat ggtaatcgat agtgcaaaag ggatcgaagc acagacaaag aaattattcc
     3421 aagttgtaaa aaaacgtggc atcccgatat ttacttttat caacaaacta gatcgtgatg
     3481 gtcgtgaacc attggaattg ttagaagaat tagaagagct attagatatc gaatcttatc
     3541 cgatgaattg gccaatcggt atgggtaaag ggctagaagg tctttatgat atctaccata
     3601 atcgtgtaga gttttatcgt ccggaaaatt atcaggatga acgattagta gaattggatg
     3661 aagatggact gcttcccgaa aatcatcctc taactgaaaa ttccttatat gaacaagtct
     3721 tagaagaagt agaactaatc aaagaggctg gagatgcatt taatcaagaa aaaattgctc
     3781 gtggcgatca gacaccagta ttttttggtt ctgcattgac taacttcggt gtacagactt
     3841 ttttagagac tttcgttgaa ttggcaccag ctccttatgg acacaaaaca caagatgatc
     3901 agatggtaag tccttatgag gaagaatttt caggtttcgt attcaagatc caagcaaata
     3961 tgaatccagc ccatcgtgac cgaatcgctt ttgtccgaat ctgttctgga acattcgaac
     4021 gaggaatgga tgtctggttg gaacgtacaa ataaaaaact gaaattaagc aatgtcacac
     4081 aatttatggc agattcgaga gaaaacgtcg aaaaagcagt agcaggcgat atcatcggtg
     4141 tatatgatac aggcaattat cagattgggg atacattgtt cgaaggaaaa ctaaaagttg
     4201 cttatgaaga actcccttca tttactccag agctgtttat gaaagtgact gcaaaaaatg
     4261 tcatgaaaca aaaatccttc cacaaaggaa tctatcaatt agttcaagaa ggagcgattc
     4321 agctttataa aacatatcta acggaagaat atatcattgg agctgttggt cagctacaat
     4381 tcgaagtatt ccaatatcgt atgttaaatg agtataacgc cgaagtaatc atgtctccaa
     4441 tgggacacaa aatcgctcgc tggatcgatc cagaagattt ggatgagaag atgagttcaa
     4501 gtcgcaatat cttggctcgt gaccgtttcg accaaccgtt gttcttattc gagaaccaat
     4561 ttgcagaacg ctggtttgcg gataaatatc ccaatgtaaa attaaaaagt ttgatgtaaa
     4621 gaagtcttga cacaagctgc cctgcatcaa gtaataagaa tagccaaaca aaaacagcct
     4681 ctcatatttt gtttagagcc gtgacggatt ttccgtgacg gctcttttta ctatattaaa
     4741 atcatagaaa tgtctaatca aaaaaatata aaaattattt tgcgtacata tgattaaaga
     4801 aaggagagtg tgacacatgg aaacagaatg gcaaagacgt gaaagaaaaa aacagaaaaa
     4861 agaagagatg ttacgattat gtcgattatt agatgtaaaa gactgggaca aaaacgagca
     4921 aattgcacaa aaagtacgat caatcatcca taaagaggaa aaagttaaaa ataaagacac
     4981 gcgtagaact gaaaatgaaa agagaaaagt acaaaagaaa agagaaaaat caaaaagcta
     5041 gaaagaaaaa gaggctgaga cagaagcgtt taactccaag aaataagctg gaattcacaa
     5101 aaatttgaaa agcaattttc gtgaatttca gcttatttcc gaaggagttg cttctgcttt
     5161 cgccgtttat acgtgtttag agcgtgagac aaaagtgttc ttta
//
LOCUS       DAJSKS010000159         5157 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJSKS010000159 DAJSKS010000000
VERSION     DAJSKS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.165"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            6..863
                     /locus_tag="ON396_002522"
     CDS             6..863
                     /locus_tag="ON396_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3114566.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            885..1394
                     /locus_tag="ON396_002523"
     CDS             885..1394
                     /locus_tag="ON396_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3114567.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            1431..1832
                     /locus_tag="ON396_002524"
     CDS             1431..1832
                     /locus_tag="ON396_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114568.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            1944..2297
                     /locus_tag="ON396_002525"
     CDS             1944..2297
                     /locus_tag="ON396_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114569.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            2331..2774
                     /locus_tag="ON396_002526"
     CDS             2331..2774
                     /locus_tag="ON396_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR3114570.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            2791..3876
                     /gene="uxuA"
                     /locus_tag="ON396_002527"
     CDS             2791..3876
                     /gene="uxuA"
                     /locus_tag="ON396_002527"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR3114571.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            3869..4582
                     /locus_tag="ON396_002528"
     CDS             3869..4582
                     /locus_tag="ON396_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR3114572.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(4789..4938)
                     /locus_tag="ON396_002529"
     CDS             complement(4789..4938)
                     /locus_tag="ON396_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114573.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT         1789 a          793 c         1137 g         1438 t
ORIGIN      
        1 gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag
       61 atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac
      121 gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag
      181 atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa
      241 gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag
      301 aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg
      361 gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata
      421 tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc
      481 cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt
      541 tcactatggg ccgtaatgct acaggcctaa agcaaaacac tggtgttcag acagcgattg
      601 aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag
      661 tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg
      721 atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata
      781 cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc
      841 tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt
      901 tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa
      961 gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag
     1021 atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga
     1081 aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa
     1141 aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata
     1201 ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt
     1261 taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata
     1321 tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt
     1381 ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga
     1441 acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa
     1501 aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag
     1561 atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag
     1621 atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag
     1681 gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat
     1741 tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc
     1801 ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga
     1861 gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc
     1921 gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg
     1981 tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat
     2041 cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc
     2101 tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt
     2161 tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct
     2221 atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta
     2281 ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa
     2341 tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg
     2401 tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat
     2461 tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat
     2521 ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga
     2581 ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag
     2641 aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca
     2701 aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg
     2761 aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa
     2821 aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt
     2881 ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg
     2941 aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac
     3001 gatgcaatca aggcagggac agatgaacga gattactata ttgataacta tatacgaaca
     3061 atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt
     3121 ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac
     3181 gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag
     3241 tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg
     3301 gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta
     3361 gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat
     3421 ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa
     3481 attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg
     3541 gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt
     3601 gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc
     3661 tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc
     3721 gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga
     3781 tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata
     3841 agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat
     3901 ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca
     3961 aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat
     4021 ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg
     4081 agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa
     4141 aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc
     4201 ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt
     4261 gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt
     4321 tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc
     4381 gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca
     4441 tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata
     4501 tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga
     4561 aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc
     4621 ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt
     4681 atttgtagtg aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat
     4741 gcgtttaaag agaagaacga gaatcgtcca tatgagggtt tccactcctt atgcagattt
     4801 ttttaattct tgcactgctt gttcgatcgt aacacctgtc gctacatgat tggtattttc
     4861 tttgtcaaaa acgacaaata gattcaaatc tgtatccaag gttactcgat attgtatatc
     4921 tttgttatag aatgtcatag ccattctcct ttctttaaat acttgctgaa aggcaacgaa
     4981 cgcgttgata acgaaattcc atttataagt ataccaccaa ttgaaaggtt ttcatagtta
     5041 agaaatttta aagtcaaaag ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa
     5101 tcaatcgcgg ttattcaact ttttcttcga ttcgaagaca atagaagaca atgaaat
//
LOCUS       DAJSKS010000160         5130 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJSKS010000160 DAJSKS010000000
VERSION     DAJSKS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.166"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            185..1900
                     /locus_tag="ON396_002530"
     CDS             185..1900
                     /locus_tag="ON396_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3114574.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            1903..3666
                     /locus_tag="ON396_002531"
     CDS             1903..3666
                     /locus_tag="ON396_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3114575.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(3706..4242)
                     /locus_tag="ON396_002532"
     CDS             complement(3706..4242)
                     /locus_tag="ON396_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCR3114576.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(4565..4987)
                     /gene="mgsA"
                     /locus_tag="ON396_002533"
     CDS             complement(4565..4987)
                     /gene="mgsA"
                     /locus_tag="ON396_002533"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR3114577.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
BASE COUNT         1539 a         1028 c          986 g         1577 t
ORIGIN      
        1 actggcatgt tttctcattt tctttttttt aaaaaaaata gtctattcaa tttctaaagc
       61 ttcttcataa taaaactttc atttatgaaa aggcttgttc tcttttcatt ctaaatttcc
      121 taagctacaa tgtggtagta cttgtttttt caattgtgga caaataaata ataggaggaa
      181 tcatatgatt cagttattac gttatgcaaa agactatcgt aagcaaatca ttttgggccc
      241 gttcttcaaa tttcttgaag ctgtcttcga acttgtcctg cctttaatga tggcttcctt
      301 gatcgataat ggactaaaaa tgaatgaccg aggaaaaatc attgaaatgg gcttatggat
      361 ggtcgctatg tctgtgattg gtctgatttg tgctatcatt tgccaatact atgcttcagt
      421 agcttcacaa ggatttggta cagagttacg taatcagcta atcaaaaaaa tcaatacttt
      481 ctctcacaac gagttgaatc attttggaac agataccttg atcacccgaa tgacaaacga
      541 catcaatcag cttcagctag ctttagcaat ggtcattcgt ttattgatca gagcaccgtt
      601 tttaagtatc gggtcagtgg tcatggcttt tgtgatcgac tgggaagtag gtctgttttt
      661 cttagcatta ttgcctattt tctcaatcat tttatttttt atcatcaaaa aaaccgttcc
      721 tctctaccaa aaagtgcaag aaaaactaga tcagctcaat gaacacgtta gtcagaatct
      781 tagcggtgtt cgtgtcatcc gcgcatttgc gcagacaaaa aaagaaacag agaagttcga
      841 taaaagcaca aatgatctag cgaacaatta tttacgtgtt tccaatatct ctgccctatt
      901 atcacctgct acaaccttga tcatgaatgt cgggatcatc tgtttgctga cagttggcgg
      961 gatcaaagta aatattggta gcttgcaaca aggacaagtt cttgctttga tcaattatat
     1021 gaatcaaatg cttttggcac tgatcgttgt ttcaaacctc gtcgtgatct tcacacgtgc
     1081 cgaagcttca ggaaatcggg tgaaagaggt gctagacaca gaaaattcga ttcttgaagc
     1141 tgaggtcagt actactcccg atttttcttc tgaagcaatt atcgcttttg accatgttga
     1201 cttccgttat actcctgatt ctggtctttc tctccaaaat attacatttc agctgaagcg
     1261 aaatacagtc cttggaatca ttggtccaac cggaagcggg aaaacgactt tgacacaatt
     1321 gattccgcgt ttctatgatg tcagttccgg tacagttata atggacggaa tggatgttcg
     1381 ttcttggcag ctggatcatc tccgtcagca aattgctctt gttccccaaa cttctgtact
     1441 atttaccggg actattcgtg aaaaccttca atggggtaag aaagacgcca cagaaaaaga
     1501 atgttgggaa gctctgaaaa tcgctcaggc agaagaattt gtccgtcagc ttccaaatgg
     1561 tttagataca aaagtcatgg aaaatgggaa aaatttctct ggcggacaaa aacagcgact
     1621 gacgattgca agagctttga ttgccaagcc agcattactg attttagatg actctttaag
     1681 tgcgttggat tatcaaacag acttggattt gcgtaaggcg ctacagactt atcttggcag
     1741 tacagtgatt attatctccc aacgtgtccg ctctattcaa gaagcacatc atatcttagt
     1801 gatggatcaa ggtaagattg ctgctcaggg tacccatgaa gaattacttg ctcaatcagc
     1861 agaatatcag gaaatcgtcg catcacagga ggaagaatag ttatgaaagt aaaaaaatta
     1921 tccttgcaga cgctccgccg attcattcct taccttactg cctatgcaaa agacatttgg
     1981 ctagctgtga tactaggaat catcaatggg attgcaacag ttgtgatgac gttacaaatt
     2041 ggtcaaagta tcgatcaaat gattggagaa ggacaggtcc gcttcaatcg actgttccat
     2101 ttacttattc tattcggtgg tatcgtttta atcaacgtag tcagtcaatg gctcatccaa
     2161 gtgttaagca atcggttagc ttacttatcc gttgcaaaat taagaaaaga tgcatttgct
     2221 catctgaatc gattgccact tcgttattat gatcaaaact cccacggaaa tatcgtcagt
     2281 cgatttacaa atgatatgga caatatttcc attgcaattt ctgcggcctt caatcaatta
     2341 ttttctggag tggcaatcat cattctttcg tttattttta tgatgcgtct aagtccactt
     2401 ctgacattgg tcgttctttg ttctacacca attattttcc ttgtgagttg gatcgttgct
     2461 cgtgcttcac aaacaaattt cgatagacag caaaggatta ttggggatat ctcgggcttt
     2521 gtgaccgaac gaatcggtaa ccagaaaatc gtgaaagctt ttcagcaaga agaagctagt
     2581 caacagcaat ttgaactgct aaatgctgat ctatatgaaa aaggacaacg ggcacaattt
     2641 tcttcttcct tgacaaaccc gtcttctcgt tttgtagatc accttgctta tttatcgatt
     2701 ggtttagttg gcggcctatt ggcattgaag agtggttcgt caatcactgt tggtgtgatt
     2761 tccagcttta cgatctactc aagtcagttt tctaaaccat ttatcgaact ttctggaatc
     2821 acgacacaac tgcaaaccgc tttttctggt ctagaacgag catttgattt gatcgatcag
     2881 ccagaagaaa agcctgattt gccagatgcc tatgccttgg aacccaaaaa tatcaaagga
     2941 aaagtctctt ttgatcatgt ttctttttct tatgacccca agcagcgttt gatcgaagat
     3001 ttcaatttta ctgcttatcc aggtcaaacc attgctatag taggaaaaac aggggccgga
     3061 aaatccacac ttgttaattt gctgatgcgt ttttatgaca caacaaaagg aacaattaca
     3121 gtcgatgacc atgacatccg ctctatccaa cgtgatacat tacgtaaaag ttttggaatg
     3181 gtgttacaag atacttggct atttgacagt acattgcgag aaaatcttcg ttttgggaat
     3241 ccggaagcaa gtgacgcaga aatcaatgat gccctaaaag cctcttatat gtatgaattt
     3301 gtagaacgtt tgccggaaaa attagatact aagattggcg gtcaagggtt aaagatatca
     3361 gatggacaac gccaactgtt gacgattgct cgaacgatga tcagtaatcc ccctatgttg
     3421 atcttagatg aagctaccag ctcagtcgat acattgacag agaaaaaaat ccaacaagcc
     3481 ttcttagcaa tgatggaagg aaaaaccagt tttgtgattg cccatcgcct gtccacaatc
     3541 aaaaatgcgg accagatatt agtgatggat cagggagcta ttgtcgaaat gggcacccat
     3601 gatgagctgc ttcaaaaaga tggttattac cgacggctct atcaagcaca attcacagca
     3661 caataaaaac aagcagtctt ctgggatggg aagactgctt gttttttatt tagcgtggat
     3721 cgctaaactt cttaaaattt ttttgtaagg aatactacgt cctacgtaat aaatggcaat
     3781 tgattgaatc ggtaaccata ttactgtttt taacaggcgg ggcgcccaaa cgacccagct
     3841 gaacaatggc acatggtaca tcaacgcaag ccataatggt gtcaaaaata agttgatgat
     3901 cagcgtgaca ctaagcgtag ccagaatggc atttttccaa gtgatctctt ttcgatagaa
     3961 aaagaatccg tagattgctc cttcaataaa tgcattcaat gtaaatccaa taaagaatgc
     4021 agatttcgag aagagggcca ttcctactaa atcagctaaa actgcgccta tccctgacca
     4081 aaaaggacca aaaagaatgc ccatgatcac ttgtggaata aatgtgacac tgattttaat
     4141 aaatggggtc tcgattgcta ccagtcggga caaagtgacc atcaatgcaa tcaataagcc
     4201 cataatagca atcatacgtg cgtctaaccg gtgtttcttc atttttagca tctcctttaa
     4261 gagctgctac atcgtatcat gcagcgaatg cgaaaataaa accgcgaaca ttacgtcctt
     4321 cgtccaagag gcaacatccc atcctcttag cacttaacgc tttattttct actctgtatt
     4381 atttttggga tcttttccca cagaaactat agcatatttt cacatttact gcaaatacga
     4441 aaaactaata ttattgttta tattatccaa tacgttcgta ttatatcgca tattatcagt
     4501 ttgtttagct tttatagcta catataggcg aacattaaac gttatttttc catacgctta
     4561 tccatcaaaa tgaaagtgga tgcttgtcta tttcatggac gattcttcga aaatctagga
     4621 agccggcttc cagtcctctt agtagcacct cagctgttcc gatattcgta gctaacggaa
     4681 tctcatagac atcagataaa cggataagtg ctgttacatc tggttcatgg ggctgtgcag
     4741 ccagcgggtc tcgaagaaaa atcaccaaat ctaactgatc ctccgaaatc atggctccaa
     4801 tttgctgatc cccgcctaac ggtccggatt taaaccgatg gatcggcaag ccggttgctt
     4861 ccgtgatcct ctgcccagtc gtcccggtag caaaaagttc atgtttttcc aatattgttt
     4921 tatacgctgt tgctaatttc accatcatag gtttttttcg gtcatgagcg atcaaagcga
     4981 ttttcataag agacactcct tttgatattg ttaacatact tatttactcc agtgtatcag
     5041 tttttatttc ttatggatat ttattaaagc ttaaaactag cacattgaca aaaaaaagaa
     5101 aaaattcttg ttcaacatga cattgaagtt
//
LOCUS       DAJSKS010000161         5114 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJSKS010000161 DAJSKS010000000
VERSION     DAJSKS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.167"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            42..464
                     /locus_tag="ON396_002534"
     CDS             42..464
                     /locus_tag="ON396_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114578.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(630..1823)
                     /locus_tag="ON396_002535"
     CDS             complement(630..1823)
                     /locus_tag="ON396_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3114579.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            2047..2424
                     /locus_tag="ON396_002536"
     CDS             2047..2424
                     /locus_tag="ON396_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR3114580.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2412..2750
                     /locus_tag="ON396_002537"
     CDS             2412..2750
                     /locus_tag="ON396_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3114581.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            2884..3327
                     /locus_tag="ON396_002538"
     CDS             2884..3327
                     /locus_tag="ON396_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HCR3114582.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            3589..4404
                     /locus_tag="ON396_002539"
     CDS             3589..4404
                     /locus_tag="ON396_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR3114583.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            4414..5076
                     /locus_tag="ON396_002540"
     CDS             4414..5076
                     /locus_tag="ON396_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR3114584.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
BASE COUNT         1713 a          883 c          975 g         1543 t
ORIGIN      
        1 cttgtatgta gtatagtaaa gtagatacag gaggagaaaa tatgcagact tttttaacaa
       61 aagtttctca aaagaaaatt aaaatgattc atttactgct tgaaacagaa cgttggtgtt
      121 caatagaaga actacaaaac gaattgaagg tatcaagtaa gtctatcttg aattatctaa
      181 cagatttaga agaattgttc caaaaatatc ctgataaggt cattttgaag aatgagaata
      241 atcggcgctt ttctatggaa aagcaggaga acttcccaat ctacattatt tatcttcatt
      301 tctacagaaa atcatataat tatcatttga ttgaattcat gtatcaacat cctgagaaaa
      361 atttggaaga ttatgcagac gctcaatata cgagtgtatc cacagttttt cgttatgcta
      421 agttattagt aaagtacttt gaaatgacgg acgtcaaaga atagtcgccg ccagatagaa
      481 ggaaattagc agttgaaaaa gctgccctgc atcaagtaat acgaccagcc aaacggaaac
      541 agtgtctcat gtttttgatt tggctggtct tattaccgca gatgcaagct tttgccacac
      601 ctctttcttg tcatttacag actcaactat caatgctgca agctttccct taatgctgta
      661 acaccagtaa gagttgtcaa gaccagcaga ctgactaggt aaataccgct caaaaacaag
      721 attacggcag ttaaactata atgatccatc aagaaaccga tgacgataga agaaaaacca
      781 ccaacagctc gtccaaggtt cataatgaaa ttattcgccg tcgcgcggat ttctgttgga
      841 tacaaactac taatcactgc gccatatcca ccatacatgc cattgataaa gtagccgact
      901 atcacagcgg ctgctaccaa actccactgg ctatgggcta acacaagtag atagacagat
      961 aaggatgaac aaattaaaaa caaagaaaag gctagtttag gccccaattt atccatgatt
     1021 atgccaaaag tcatcatccc caaagacatg cctaaaatag tactgaccat ccacaatgaa
     1081 gacccagaaa ccgaaagccc taatcgattt tgcataattg aaggcagcca attcattaac
     1141 ccaaaatagc cggcaatttg gacagttacc atgatactaa ggccacatgt ttgccaagca
     1201 agatgttttg attcaaacag tctgctaagt ttggttttct tttcggcaga ttgtacagat
     1261 ttgaattcct ttggttcagt caagccgcga cgaatgaaca agaccatcaa caccggcagt
     1321 acacctacga catacaacat cttccaacca aacgttggaa tgatcacagc agctaaaatc
     1381 gcagccaaag ccgcaccgac ctgcgcacca ataccagcaa ccgaagaagc tcgtccaatt
     1441 tgtttttttg aaaaactttc ggaaatcaag gacatacacg ccccgtattc tcctcccgct
     1501 cctacacctg cgataaagcg aaagagataa accaagtaga tgtttgaagc aaaatacatc
     1561 aataaagaag caatcgaaaa cagaaggacc gtttgagaaa atactttcac acgtccatat
     1621 ttatcagcta tgatgccaaa aaaaatacct ccaatgagca tccccaaatt agtgatcgtt
     1681 gaaatgaaac ccgcttgcgt accactaata tgaaaagaag taatcattga agataatgaa
     1741 aaagctaaaa acataatatc catattctct aaagcaattc ccgccgatgt cgataataat
     1801 acctttcgct gatacgttgt catttttata ccccttttcc taaaatttcc ctaaattgtt
     1861 aaaagtatat ccaaaaaaga ataaaaaaac aagacttttt tatttgaaat aatacacaaa
     1921 caaccgatat agataatcca gcttagtttt aatagaaatg agtattgact tggagtacac
     1981 ttaaagttgt agaataaata aaatacataa gtaaagtggc aagaaattaa gagaaagagt
     2041 gatcagatga atatcaaaca agtcagtgaa gaaaagggca tttcggccga tacattgcgc
     2101 tattatgaac gaatcgggtt gattccacca gttaatcgga caaaaggcgg aatccgtgat
     2161 tatacagaag aagacctaaa atgggtcgac tttacgatct gtatgcgaaa tgccggatta
     2221 tcgatcgagt ctttgattga atatattcgc ctatctagcg aaggagaagc aactgtattt
     2281 gagcggcgta agcttttgat tgaagaaagt gaacttttga cgaaagaaat tgctgaaatg
     2341 caagagtgtt tggaacgttt acaaggaaaa atcaagaaat atgatcgtgt gttatctaga
     2401 aataaaatgg aatgtatcaa gtaaaaaaat caaggagcat cgctatggag ttacgcttac
     2461 taaaatattt ttggacaatt gcagaagaag gaacaatctc tcaagccgca gaagttctac
     2521 atcttacaca accaacctta agccgacaac tgaaggagtt agaagaggaa ctaggaacag
     2581 aactgtttgt acgcgaaaac cgcaaaatga tcttgacaga agcaggttat tttctaaaaa
     2641 agccgagcag aagaaatttt agatttaacg aatcaaacca ctcaagaatt cgagaacaga
     2701 aaaaaacaat tattcagcgg ccgatttacg atcggctgtg tggaagctga taattcgaat
     2761 acattggcac tattactaga aaagttgatc cgtgattatc ccagcgttca atttcatatc
     2821 cacagcggaa caagtgatga tatcacggag tgactagaaa aaagcctgtt agatttagcg
     2881 attttgctag atccagtaga tacagagaaa tacgaaacga ttttgttacc aagaaaggaa
     2941 aaatggggat tgctcatttt agaaaattct gttttagcaa aaaaagacgt gataacaaaa
     3001 gaagactttg cgaatgtacc tcttttgatc tcaaagcgtc cagaaataca gcgaatgttt
     3061 gctgactggt taggcataaa aaaggagcag ttgctgatca caggaacgta caatttgatt
     3121 ttcaatgtat ttgcgctaat tgagaatcag gtaggtgctg ctttagtcat agaaggtgct
     3181 gtaagtcatc tttcaaagcg agtgacattc gttccgcttg acccaatgat tgaaacgaac
     3241 tgcatgctag tctggaaaaa gaatcggatt ttatcacctg tagtcaatga attgattcac
     3301 cgtttcaaag aagcttttga acagtaataa gtatctctaa tcgaagaaat ttcaatacaa
     3361 aagcgagacg tatgacagtc ttgcttttgt attttttcat ttttaaatca aaaaaaataa
     3421 agagatttac ataacttgtt aaataagcgc ttacaaacat aagaagaacg gaaatgaatc
     3481 agctataata accgttgttc tggattgtaa tcattacaaa gaaatgaaga ttataacttt
     3541 ggaggtgttc gaaatgaaag aaaaaaagca aaaattcgtt caaaaaacat gccctttatt
     3601 caatggttgg gtatgattct gctgattgta ttagctttat tgacgatcat tcctttttta
     3661 tggatggttt ctgcttcatt caaagcaaat aatgaagtgt tcacgattcc gattcaatgg
     3721 atccctaaaa cttggcattt agagaattat ctggttattt ggtctcgtat tcctttagtg
     3781 acatttttta agaatacagc ttttttaagc gttgtgatca cgttgattca gttgtttact
     3841 tctagttttg ctgcttacgg gttttctaaa ctaaatttta aagggcgcga tattttattt
     3901 ttagcatata tcggcacgat tgctgtccct tggcagtctt atatgatccc gcagttcatt
     3961 atgatgagaa aagtgggttt aacatatacg ttgtggtcgt tggtgctatt acaagcgttc
     4021 aatgcttttg gtgtgttctt gttgaagcaa tactacagta gtatcccaga ttctttatgt
     4081 gagagtgcgc ggatcgatgg cttatcagag tggggaatct atcgcaagat cattttacct
     4141 cttacaaaac cggcgattgc cagtttgacg atcatcacat tcgttaatac atggaacgat
     4201 tatatggggc cattcattta tttgtcttct acggaaaata aaacaatcca gttagggttg
     4261 aaaatgttta ttgggctatt tgatgcagag tacgcattaa tcatggctgc ctcagttgtt
     4321 tctgttcttc ctgttgcttt tatattttta gcgatgcaaa aatattttgt agaagggatc
     4381 gcttcatcag gaatgaagga ataggaggtg attatgaaag agcagcgttt tgacaaaaat
     4441 gtttatatgg aattctttta ttggttgtat caatcattgg caagtagcct ttgtttttgg
     4501 tgtgtcaata tcttgtatat agcagctgcg ttccttttgt cgatagatgg aaggaatgtc
     4561 ttttccttta ttttttcttt actttttatt ggtccaggtt gcatcgtgat gataggtatg
     4621 acagaagaat tagcaaaaga aaaaagtttt tcaccagtac gtttgttcat aaagacgttt
     4681 cgccaatttt ggctaaaggg atttttttat tggctatttg cttggatagt tagtgtgata
     4741 atgatttttg attgtttctt ttttatacgt ttttcttatg gaaaatggtt gattccgttg
     4801 tttgttttac ttgcttgttt atcagtttcc tttagcatca actactggta ttttcaggtg
     4861 agaaatccag cgtcgaaacc aaatcaagtt ctacggatag cgttctacta tacgttgaaa
     4921 aaatggtatg tgagtttact agatttttta ctattgacaa gtttgtttct cttctttttc
     4981 gtaaagcccc aatggtgtat tctactaggt ccttctatcg tatttggttt gatttacttc
     5041 aacaatcgta aattgatgag aactatggat ctgtagactt tataaattga aactttcaat
     5101 taagaatgac agtt
//
LOCUS       DAJSKS010000162         5080 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJSKS010000162 DAJSKS010000000
VERSION     DAJSKS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.168"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            206..1480
                     /locus_tag="ON396_002541"
     CDS             206..1480
                     /locus_tag="ON396_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3114585.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            1534..2961
                     /locus_tag="ON396_002542"
     CDS             1534..2961
                     /locus_tag="ON396_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCR3114586.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEVKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            2974..3846
                     /locus_tag="ON396_002543"
     CDS             2974..3846
                     /locus_tag="ON396_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR3114587.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            3941..4936
                     /locus_tag="ON396_002544"
     CDS             3941..4936
                     /locus_tag="ON396_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3114588.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKNN"
BASE COUNT         1753 a          699 c          991 g         1637 t
ORIGIN      
        1 tataaaaaat agtgccatta aaaaagaaaa gcgacacacc aataaaagtc atatcctgtg
       61 acattatatg gcattatcca aatagaataa ccggttgaca tgtaagcgga tttcttttat
      121 aattttagga gtacaagtcc ttttctaaaa atgttaaccg gttatcatga aaggggatac
      181 aaatatatcg ttggaggata aaaatatgtc aaaagttaac gtatttaaaa atcaatctta
      241 tttacaaagt tcagctacat tattactatt ttttgcttct tggggtgttt ggtggtcatt
      301 ttttcaactt tggctaacat ctgaatcaaa tggtttaggg ttatctggca gtgctgtagg
      361 aacagtattc tcggcaaatt cgttagttac cttaattttg atgtttattt atggaacatt
      421 acaagataaa ttgtatatta aacgaaattt attaattttt gcttctgtat tagcgacact
      481 tgttggacca ttttttatat ggatatatgg gccattgcta gataacaatt ttaatttagg
      541 cattattatg ggagcgctat ttttgtcagc tggatattta gcttctgtag gagtttttga
      601 agctgtgtca gaaaggttta gtcgtttatt tggctttgaa tatggacaag caagggcgtg
      661 gggatcattt ggttatgcct tggtagcgct tttggcagga tttttatttg taaaaaatcc
      721 tcatttaaac ttttgggcgg gatctttctt tggttcttta ctattgttaa atttattatt
      781 ttggaaccct aaagttgaac gggaagcaaa tcaaaatttt aatcaagaac aagctgaatc
      841 aaatagtatt ccttctttaa aagaaatgtt tgatctaatg aaactgcctc aattatggac
      901 gataatcatc tttattgttt ttacatggac attttatacg gtattcgatc aacaaatgtt
      961 tccgggattt tatactggtt tgttttcaac atcagctaat ggtgaaaaaa tatatgggac
     1021 attgaatgct attcaagtat tttgtgaagc gttaatgatg ggaattgttc caatcattat
     1081 gagaaaatta ggggttcgaa atactttgtt attaggtgta accattatgt gtgtacgaat
     1141 tggattgtgc gggtttgcct cgacaccatt atctgtttca tgcataaaaa tgttgcatgc
     1201 tttagaagta ccattattta cattaccaat gtttcgctat tttacacttc attttgatac
     1261 aaagctatca gcaaccctct atatgatagg atttcagata gctgctcaaa ttgggcaagt
     1321 gattttatca acaccattgg gaatattaag agacaacgtt ggctatcaac caacatttaa
     1381 aattatttct cttattgtat tactagcagg catatatgca ttctttattc ttaaacaaga
     1441 tgatagagat gttcaagggg atccatttat tcgaggataa tggaaaccaa cagaataaaa
     1501 aacaatttta tctagaaagt gagcgaatat attatgacaa aaagagctat agaattaaca
     1561 aatacacgtt atcgtttggg atatcatgtg tctgcaccat caggatggat taatgatcca
     1621 aatggatttt gctattttga tgggtattat catgtgtttt atcaacatca tccatatagt
     1681 gctgaatggg gaccaatgca ctgggcacat gctagaagta aggatttagt tcattgggaa
     1741 tctttaccac ttgctttgac tcctggagat caagaagatg aaggtggttg tttttcagga
     1801 agcgctattg aaaaaaatgg tgtgttgtat ctgttctata caggacatca ctattatgga
     1861 gacaatgatc cagatcattt ttggcaaaat caaaacatgg cgtatagtac ggatggcatt
     1921 cactttacta aatatgaaaa aaatcctgta atagcaaaag caccagaaga taacacacat
     1981 catttcagag atccaaaagt atggaaacac gatgaaaaat attacatggt tttgggtagt
     2041 caagaaaaag atggattagg tcgtgctatt gtttatcagt cgttagattt attaaattgg
     2101 gattacattg gaccaatttc taaagcgaag gaagtactta cagaaggatt tatgtgggaa
     2161 tgcccagact tttttgaatt aaatggaaaa tctattcttt tattatcccc tcagggaatt
     2221 gaagcaaatg gaaaagatta tttaaatcta tatgaaacag gctactttat cggtgattat
     2281 gattatgaga cagcaaaatt cattagagga aatttttatg aattagataa gggacacgat
     2341 ttctatgcaa cacaaacaac tttatctcca gatggtagac gtattgttat ggcctggatg
     2401 gatatgtggg aaagccctat gcctgaaaaa gtagacggat gggcaggagc attaacgctt
     2461 cctcgagaat tagagttaat tggagatcgt ttgtatatga aacctgtgag agaattagaa
     2521 caacttcgag ttggcagcgg cgttgaaacg acagttaatt tggcaggaga aataataatc
     2581 gctcaagatg cttcattcaa agaagctcta attgatgtac tattgactaa atcaaaaaat
     2641 caagaagtca aattttcatt aaaaacaaaa gatgaagagt taatcaatat tttttattct
     2701 aaggataaaa atgaatgtac cttaactcgt aacggcaaaa atgatccaag gtatggaacc
     2761 attcagatgt gtgatagatt atcacttcga gtgtttatcg ataaaagttc ggcagagatt
     2821 tttattaatg atggcgagct tgtttttaca gagcgttatt atacagaaaa tcaaccaagt
     2881 atgcatttga gtgttagtga agaagctttt gtctcgagta caatttatca tctaaaaaaa
     2941 gatgcaattt cttacgagta aggaggtttt atgatgttaa ttggtagcat agaagcaggt
     3001 gggactaaat ttgtatgtgc cgtgggagac gaggattacc gagtacaaga ccaaataatt
     3061 tgtccaacga ccacccccag ggaaacattg aaaaaaactg ttgactattt taaacagttt
     3121 gatataaatg cattaggtat tgcatcattt ggtccaatag aaattcgaaa aaattcacca
     3181 aaatcaggat atattacgtc tacaccaaag gtgggatggc gaaatgttaa tgtgttaggt
     3241 tatttacaag atgagctgaa tgttccaatg tcttggacaa cggatgtgaa cggatcagct
     3301 tatggagagt acatcatgtc cactttatcg aatgaaaaaa taaattcatt agtttattac
     3361 accgttggta ctggcgttgg tggtggagct gttgttgacg gaaaactatt aggatcgttt
     3421 ggtcattcag aaatgggcca tacgtttgta aaacgccatc ctgatgatat tgattttaaa
     3481 ggaatttgtc cttatcatgg tgattgttta gaaggcttag ttgcgggacc aacgtttgaa
     3541 gcacgttttg gaaaaaaggg tgaagacgtt tctttaacgg atcctatttg ggatgtaatg
     3601 gcctattatc tagcacaagc tgtgattcaa acaacattga ttatacgacc ggataaaata
     3661 gtatttggtg gaagcgtagt aaatgaaaaa ttgttagaaa aaattcgtgt taatgttaat
     3721 aaattattgg atgattacgt ggaaatacca ccgttaagta aatatattac gcgtccttta
     3781 attaaaaata atggatcggc aacactagga aactttgctt tgggcttgaa tcaattacaa
     3841 gaataaaaca tagaaaaaat atcgattatc aggtatagta gttctataga gttctttttt
     3901 tcgatagtta attattgcat tatttaggag agacaagaga atgcgcccaa aattagaaga
     3961 tgttgccaaa agagcaaatg tatcaaaaac aactgtttca cgtgtattaa acaatcgtgg
     4021 ctatttaagt caaaaaacga ttgataatgt ttataaggct atagaagaac tcaattatca
     4081 accgaacgtt gtagcgcgtc aattatttca aaagaaaact aacattgtcg ggttactatt
     4141 tcctacagta gccaatcctt tttttagtga actcgttgag gcattggaaa aaaagttata
     4201 tgaaattggt tataaggtat taataggcaa ttcgatgaat aataaagaaa aggaaacaaa
     4261 ttatttaaat cagttattgt ctgatcaagt agatggttta attgttggta cacataacca
     4321 aggaattcag gaatataaat atcagaattt accgattgtt gcaattgatc gagtgatgaa
     4381 tgaagatatt ccagttgttg aatccgataa ctacaatggt ggaaaacttg caaccaagtt
     4441 attaattgca caaggtgcaa aaaatattat tcatacaaac ggtccaattg acttacagac
     4501 tccggctaat cgtcggagac tagcctatga agatactatg aaagcatatc agttaattcc
     4561 acgaactgtg acattagatt tcaatatttc ttatgtaaag aaaaaacaga tttttttcca
     4621 aatgtttgag gattatccta aaatagatgg tatttttgct tcaaatgaca tcgatgcggc
     4681 cctaatatta caagtagcaa aagaaaaggg gttaaatgta ccagctgatt tattagtagt
     4741 cggatatgat ggtactttaa tgactcggag tatattacca gatctgacaa cagtaataca
     4801 accaattaat gatatagcag acacagctgt tgctattttg atgaaacgaa taaataaaga
     4861 agaaacaaaa aaggaatata ttttacctgt cacattatgg atggggaaga catcagtaaa
     4921 gaaaaagaac aattaaattt ataaagacta tcaatgataa tttcattatt aattcgtgat
     4981 ataaaaaatt gttaaatctc ttataataga ctatgataca aaatcatgct tttaatctgt
     5041 gtagatgttg ccaaatattt gaaagacata gaacttgtca
//
LOCUS       DAJSKS010000163         5047 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJSKS010000163 DAJSKS010000000
VERSION     DAJSKS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.169"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..606
                     /locus_tag="ON396_002545"
     CDS             <1..606
                     /locus_tag="ON396_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR3114589.1"
                     /translation="FILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYCWK
                     VFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLLPI
                     IWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLSRV
                     VSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            679..1608
                     /locus_tag="ON396_002546"
     CDS             679..1608
                     /locus_tag="ON396_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCR3114590.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1677..2503
                     /locus_tag="ON396_002547"
                     /pseudo
     CDS             1677..2503
                     /locus_tag="ON396_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            complement(2551..3729)
                     /locus_tag="ON396_002548"
     CDS             complement(2551..3729)
                     /locus_tag="ON396_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR3114591.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            3752..3925
                     /locus_tag="ON396_002549"
     CDS             3752..3925
                     /locus_tag="ON396_002549"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3114592.1"
                     /translation="MCCGNSILLKGLPFFYLHKIFYTLGLKERGLSGVKMVISDAHIG
                     LTKAVKEYLLCAS"
     gene            3956..4357
                     /locus_tag="ON396_002550"
     CDS             3956..4357
                     /locus_tag="ON396_002550"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3114593.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4564..>5047)
                     /locus_tag="ON396_002551"
     CDS             complement(4564..>5047)
                     /locus_tag="ON396_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR3114594.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1579 a          868 c          979 g         1621 t
ORIGIN      
        1 tttatactac ctacacttta tagcctttta tgtgtaatgg caccatgtat tatttatatg
       61 gtattttttt gcaaaaaaaa taccctgaaa cacaatatcc tgattttgat ttttatgctg
      121 tactgttgga aagtttttga attgactggt acaggtggac taacagatat tttttataga
      181 cctgatacag gtaggaaatt ttctctagta cagggcagtg ttaatcttgt accattcagt
      241 ggactaaaca ttagctttat attaaatata gtaatgtgtg tgcccttagg attcttacta
      301 ccaatcatat ggaaaagtta taggaaaata gttcctgttt tcttggctgg ggctctattt
      361 tcactaatta ttgaagtctc acaaattata actacaagag caacagatat tgatgattta
      421 atagcaaata cctttggtgc ggttataggc tttatactgt gggctgtttt aagtagagtg
      481 gtgtccttga aggacaagtc atctacagta gattatttag aaccttatat ttatatattg
      541 attggtttta ccggaaaatt cttactgtat tttcctttct ggtttgcttt tgaagtatta
      601 tcctgataat tccatagaaa ataatgagtt tgagttgaga aagaatattc ttgtacacaa
      661 ctctggaaag gaggaagtat gaaaagattc ttaaaatggg ggcttgttct tttagcgtct
      721 ttgattgtga ttctaatgtg tacagcggtt gttatttctt atgtgaatcg gccgtatgca
      781 agagctgttt atgataggat taaacgcatt cttgacaaag atttttatga ccatctatat
      841 ctaggttctt catttgaaat aataaacctt gatacaatgg atattcatag ctatatgaaa
      901 aaagaccaag aagaattacc cgcctcattg agcaaattat ttacgattga ttatgcgctt
      961 agtgtcctaa gtttagatga acagattaca attagtccta ctgcacttga catgcagaaa
     1021 gctgactcat ctactgcaca tcttcaagaa ggagatattt tatctgttta tgacattatc
     1081 tgtgctatgg tcttaccctc aggaaatgat gcagcttata gtttggcaat ttatacaggt
     1141 ggaaaacttg atcctgatgc aaagaatgat gaagaacgct atcaaagctt catgaaaaac
     1201 ttagatcgtt atctaagtga gcaaggctat aaacatactc attttactga cgctgctggc
     1261 tatgactcca atgctacaac atcagcggaa gatttgacaa aagtagctgc aaagctacta
     1321 aagaatgata aatttgcaga aatagttgcc aaggaaactt atagagcaaa atcaattaag
     1381 ggaaatgttt ataactggaa tactacgaat caatttatgc aacaagataa tagatacttt
     1441 actgaaggtg taaaaggtgt aaaaactgga tcgtttgatg ggagatacaa tctatgtgtg
     1501 tattatacat ctagttctga taaccactat ttacttgtaa gttttggttc agagagtgat
     1561 agtaggcgtt atgtagatgt tcaaaatcta ttgcataatc tgccttgaaa tttcgtgcct
     1621 ttaagcgcgt atctaaagtt tctttgaaaa gtttgagaat gaagaaagtg agcagtcgaa
     1681 aaacggttgc tcactttttt gccctaaaat cgaaaaaata taaaaaagtc ctttacaaca
     1741 cgtctcagga aaaactgcta agtcaattct aatatcttgt ggagcaagac ttgcaccttt
     1801 tgatataaga gagcttagag aactaatgag cgaagatgaa ctagaactcg ataaaattgg
     1861 agatagaaaa actgccttgt ttgtaataat atcagataca gatgatacat ttaattttgt
     1921 agtttcaata atgtattctc aattatttaa tctactatgt gataaggcag atgatgtgta
     1981 tggtggaaga cttccagttc atgttaggtg tctacttgat gagtttgcaa atatcggttt
     2041 gattcctaaa tttgaaaaac taatagcgac aattcgttca agagaaatat cggcaagtat
     2101 aatactgcaa gcacaatctc aattaaaagc aatttataaa gaccatgcag atacaatagt
     2161 tggtaactgt gactctacac tttttctagg aggaaaagaa aaaacaacag taaaagaatt
     2221 atcagaaacc ttaggaaaag aaaccataga cctatataac acatcagaaa caagatcaaa
     2281 tcaaaaatct tttggtctaa attatcaaaa gactggcaag gaacttatga gtcaagatga
     2341 aataactgta atggatggtg gtaagtgtat ttaccagcta agaggagcaa gaccttttct
     2401 atctgataaa tttgatatta caaagcataa gaattacaaa ttgttggaag attatgataa
     2461 gaaaaacttg tttgatgtag aagagtattt aaaacaaaga tagcgtaaaa tattttgtgt
     2521 aaataactcg tccttctgca aaataatggg ttactcagta aacattgaag ctaatgtatc
     2581 cgtcacttgt tggaagcctt tatggcttct atttaagaat ttttgattgt atgtatcaaa
     2641 gatggatact aggaaacgct ctagtgattc ttcattttga aactgctctt ttctgtggct
     2701 gtatttcttg atttgcttat taaaggattc aatcaagttc gtagagtaaa tagtcttgcg
     2761 tatggctaat ggaaaatcat agaaagtgag caggtaatcg ttcgaaagga tcgattccac
     2821 catttttgga taactggttt tccatttttc cgcaaaagca ccacgtgctt ccaatgccgc
     2881 ctttttagat gacgcttgat acaccatttt aaaatcatca cagacttcct tacgatcatc
     2941 gacacgcact ttgtgactga tattacggga aacgtgcaca cagcaatgtt gaaaacgggc
     3001 tttggggtag aaccgctgca cggcccctgc catccccttt aagccatccg tgatgaaaag
     3061 gaggacgttt ttcacgccac gttcctggag gtctattaaa atctcttccc aaatcgtgat
     3121 agattcagtt ggtgcaatcg catagctcaa cacttccttt gaaccgtccg ggcgaatgcc
     3181 aactgcgata tgaatggctt ccttggcgac ggttttccgc tttaaaggga tataggttgc
     3241 gtccatataa atagcggcat aacggtcatg aagctcacgt tccttaaatg aagtgacttc
     3301 ttccgtaaat gatttagtca tattggacat ggtttggggc gtgtagtgat gcccatacat
     3361 tttctcaatc aagtctgcga tttccgacat ggtaatacct tttcggaaga ggtgaatgac
     3421 ggtctcctct aaggtatcat tcgtccgtct ataagcagga acagtctgtt gcttgaactc
     3481 gccgttgcgg tcgcgcggaa tctggagatg aagttccccg tactcggtct tgaccgtacg
     3541 gtcataggag ccgttacgag aattacccgt gttaaaacca atgcgatcgt acttttcgta
     3601 atcgaggaaa gccgttaact ccgttttgag aagcgtattg acagcttttt ccaaatggac
     3661 acggaataat tcattcaaat cgcctttatt cgctagagtc tttagaattt ctgtagtaaa
     3721 atcgttcata tggaagtcct cttttctgtg aatgtgttgt ggtaactcta ttctactgaa
     3781 gggacttcct tttttctatt tacacaaaat attttacact ctcggattga aagaacgcgg
     3841 tctttcagga gtaaagatgg tcatctcgga tgcgcatata gggctcacaa aagctgtgaa
     3901 agaatacctt ctttgtgcga gctagcagag atgccaagca catctccttc gcaacatttc
     3961 cgacaaattc tccaagaaaa tctcaagcga tatcaaagaa gactttaaag ccatcttcaa
     4021 aacggcaagc atcgagctgg ctggagaacg taaacaactt attatggata aatatgggga
     4081 agacaagaaa gtcgcaactg cctgtaaaat attggatgag ggcttcgaag atgccatgca
     4141 agtaatggtt ttaccagaaa atatccgtca acgattgaga acaacgaatg ttcttgaaag
     4201 actaaatgaa gaagtacgcc gtcgtgaacg tgttgtccga attttcccaa attgtgattc
     4261 tgttattcgt atcatcggtg ctttattgat ggaaaaggat gacgaatgga cttctgcacc
     4321 tagaaaatat cttgaatttg atcgtaacga gatttagcac attaagccgc caatagtata
     4381 atatactggt gagaagtatc aagagacgct tcgctttttg ctcttgacac ttcttaccag
     4441 catattggac cgtagctagg ctcaacaagg caaaacatcg tactttgaga tttccttttt
     4501 acacaagatt ttggacttga cacagcccct atggttctta ccttgtttat gcttatttaa
     4561 atcttacttt attacagtac tttcaaactt cgttttgtaa gccgggtatt ttgatatttc
     4621 ataattatgt ccctcttttt gaatatattc tttgatatct gcttgttcat ctacactttc
     4681 tttgcgtaaa atacgttcga tcattgagca gcagtgcatg taaagcaaga acaattgatt
     4741 gtttgaaaat tcgatcccca ttagtttttc gagttctttg acggcctttt gtaccaatct
     4801 caaaaccttc attgcatcaa ggatcgttaa attttctacg atcttgttaa tcgagaaatt
     4861 aaagtattga gcacaattat aggcgataaa tggagcattt gattctatca cctgttgttc
     4921 cttggcgtat tcataaattt tttctgccaa aaaggttttt cctgaaccgc ttacacccat
     4981 caataaaatc ggtaatccgt tatgaggata caagacagcc gtcttgattt gatcgatcgc
     5041 ttccttt
//
LOCUS       DAJSKS010000164         4979 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJSKS010000164 DAJSKS010000000
VERSION     DAJSKS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.170"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            63..1204
                     /locus_tag="ON396_002552"
                     /pseudo
     CDS             63..1204
                     /locus_tag="ON396_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1330..2409)
                     /locus_tag="ON396_002553"
     CDS             complement(1330..2409)
                     /locus_tag="ON396_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR3114595.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2512..2952)
                     /locus_tag="ON396_002554"
     CDS             complement(2512..2952)
                     /locus_tag="ON396_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3114596.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3009..3419)
                     /locus_tag="ON396_002555"
     CDS             complement(3009..3419)
                     /locus_tag="ON396_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR3114597.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3599..4033)
                     /locus_tag="ON396_002556"
     CDS             complement(3599..4033)
                     /locus_tag="ON396_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3114598.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4133..4960)
                     /locus_tag="ON396_002557"
     CDS             complement(4133..4960)
                     /locus_tag="ON396_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR3114599.1"
                     /translation="MWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGILLEAALG
                     FDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITSDRLLSVI
                     FGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVSLNLFIFG
                     IEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIGRGLTLLE
                     AKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGFTYHPIE"
BASE COUNT         1814 a          959 c          844 g         1362 t
ORIGIN      
        1 tataggtaga atctctacta tcttatttct tgcatacaaa aaaatcaagg agtgagagag
       61 ttatgagata ttcaaccatc ttaatatcaa catttgcagc cttaagctta tcgcttacta
      121 taaatcagac agtccacaca gaggagacag aaacagccaa atggacagaa accaagtggg
      181 ccaacgtcaa tcgaattacc tttcaggatg aattttcatc cttcaattca ggagtcgcaa
      241 aattaagaaa aggacaaaca gcgaccatca aagcaaaaat tgactattcg ggagacttag
      301 cacctatact acaatcagct gttacatttg agaatgttga tcccactttg tcgattggct
      361 atgacgagaa cactttgact atccaacaca agcaagcgat atttaccttc accgttacat
      421 taaatcagga cttgttaaaa ccagcgcttt ttactgtcaa ggtatcagat ggactaccga
      481 acgatcatca tttaacgccg tatagtcagc ggcagacaat tgagcaagat tcagccatcg
      541 actcaggagg agatcttgtt gaagaaccaa cagataaacc agaaaatgaa aataagccag
      601 aagtaccgcc tacagaaaat ccagacggag aacagaaacc ggaaatagaa cctggagaag
      661 aacccgatac agaaactcaa ccagagccag acaataaatc aaaacctgaa ataacccctg
      721 gagaaaaacc agatgtaact ccagaaccag atacggattc tggaaaccaa acagtaccgg
      781 aaacaaatcc agacacagat aatgaaactg aaaatccaga aaaaccagag gtagatccag
      841 aagaaaaacc agatgtgact ccagaaccag atacggacgc aagagaccaa ggaataccag
      901 aaaaaataaa caaaaaaaca attcaagagg atggaaaaaa agagagtaaa aaatcaaatc
      961 tagcaattct aaaaataaat gaagagcagc tgaataaaaa atcacgaata tttgatagcg
     1021 cccaatcagc tgaaacctta aaaagtagca aggatacaac ctttgctgtc ctgaaacgaa
     1081 aaataaacag ctaccaaaat ccggagagtc acaaaacaaa gttatactgt ggagcggaat
     1141 cattctatta agtatagcaa caatgctttc tgcaaaaaga tttaaacaaa acagatctct
     1201 ctaacatttg aataatgtct gtgaacgcat ttttcaaata aaaagaaata tccagaaaat
     1261 gaccccaaaa taccagattg aaatctgata tttggggtcg ttcatacttt atctttcttc
     1321 atatttattt taagggatat ctgtttcttt tggaagatgg ttcgggatct ttttcacttt
     1381 caatacatct aaaaagaagg taaagatcgg aatgccgaca atcaagcccc aaacgccaaa
     1441 gaaccgttcg ctaacaagta agaccacaaa tgtataaaaa atcggcagtt ctgttttgct
     1501 ggacatcaac ttcggattca gcacataaga ctcaattaga tggacaatca aaatcgtaat
     1561 caggatataa acaacatctc ggatgcctcc ttgggaataa gctatgaaag atagaggaat
     1621 acaagaaatg ataacacctg ctactgggat cagacttaaa atgaaaatca tgatcgctaa
     1681 actcagcagc tgatgaaagc caaatacagc caaggcgatt gtagtgagca acgtatttaa
     1741 aagagcgaca acaaattgag cttctaatac caaaccaaat gtattgacaa acttatcggc
     1801 aaaatagtaa atatcttgaa agaaccaaga aaattcactt tttaaaaaca atcttgagaa
     1861 tacaattgtt tttttctttt caatcataaa aaagaaactt aagatgaatg acatagcaaa
     1921 cgccactgcc atttttccta tatcctgtaa atagccaagc gcaatcgaag cgccattttg
     1981 aagctgttca aaaaaatcgt ttcgctcaat ataagtatgg atatattgta aaacaggatc
     2041 tgcatctttt ggctgatgct cgtaaaaatt aataacggac tggatcatgt caaatgtttg
     2101 atgaataagc atcgggatat actttgtgaa tgccagatag ataaggtaaa cgatcagtgt
     2161 ataaatcatg atagctaaaa cacctgtagg gattcggaca aatcgctgaa tgatttgaac
     2221 aagatgaatc atcaaataag taaagataaa tgtgagcaaa atggttgtaa tcatacttct
     2281 aactaagaaa agaacaaaaa cgacaaataa taaaacagtg agacggcgta ttttttcatt
     2341 cgccaaaaaa cgctgataaa cggtcaaatc gacaacctcc aaataacaaa aaataaacac
     2401 gttattcatt atatgatgga atatgaaaat aagaaagagc aaaaagaaaa accgctaaat
     2461 gacgttatct tatgagttgg ttttcctcat aaaatcgttc atctagcagt ttcagccctt
     2521 tttctggtct tgatgaaaat tatctagcat ttttcgggtc agatgttgca gtgtatccaa
     2581 ttcttcctgt tcaagtggaa gataagactt gatcttttgt ggaatacctt ctgcttgttt
     2641 ttgcaacgca gcaccttgag cagtcagcgt aacatttacg attctttcat cacccgtttc
     2701 acgctgacga ttaatataat ttttttctgc taactttttt agtaagggag tcagcgtacc
     2761 agaatccaga cacaaatcat ttcctagctc tttcactgaa gtctttttct tttcccataa
     2821 aactaacatc acaagatact gggtataggt aagattcagc ggcttgagga ggggagtgta
     2881 gtgtctaatg atttctttag aggcggcata taaaggaaaa catacttgat taactaaata
     2941 tagatcttcc atttttccac ctccgctgtt ttactatagt gtatcatagc ggatttacac
     3001 aaaaaagtct attcactaac tgctttcaga gtaacttcta tatttccagc tgtagctttt
     3061 gaatacggac acactttatg cgctgcctct aataattctt gcgtttcttc cagagaaaga
     3121 ccttcgatag atccttcaat ctctacacct aagacaaagc tcgtttcact ttctgaataa
     3181 agggaaactt gtgcactgac agtagaagcc ccagagatat ttttttgctg tttgatcaat
     3241 tctaatgcac tgttaaaaca tgcactgaaa ccagctgcaa acaattgttc agggttggtt
     3301 gcattttcga cgcgtttacc aggagctgca atattcaatt cgaatgtacg atcgggatta
     3361 tgcacttctc cagagcgtcc gcctgtgttg atcattttcg tagaatacat ttttttcata
     3421 aaattaccac ctttaattat attttataca attgaatttt acacaatata attgtggaaa
     3481 gcaaagaaat ggattggcat aagataaata agagaaagca catgaaacag acgcgctttc
     3541 tcgaatagca aacatataca aaaaatatca gagacaaaaa ataatctcat cataaacatt
     3601 acatatattt ctcaaaaagt tgttttcttt cttcaaaaat tacttcacga tggacctgat
     3661 gaagcatcca taaataacca aaaggatctc tgaacattga gttagtcaca ccaaagtcca
     3721 tcatttctgt catttcttga atctctacac agccatttcg taatgcttta ttatgtgtgt
     3781 cctcgatatc agagacgatg atattgaacc agatcgtatt tggatgttca gcagtaggag
     3841 cagtcaaacc aagagacgtg ttttcatcca gcatatggaa tctcgtacca tacaaggtaa
     3901 aaatgacttc attattgcct ctagggtaat ctgttgcctc aatcagttgt atttcatcaa
     3961 agattgtttg atacagttct agtgctttca aactatcatt taatacaaaa tcgatttccg
     4021 ttcctttatg cataaagatc tcctctcctt ttcagtcgaa cagttactag attcttttat
     4081 tataccagct aaagaaaacg cattcaaaaa aatagcggat ttacttacca actcattcga
     4141 ttggatgata agtaaatccg cgtcccatta cttctgctac gctgctgaca atgacaaagg
     4201 cacttggatc gatttcttct atcaatggtt tcattcggga aaattcatga tcagtcacaa
     4261 cgatcaataa aacctctttt gactttcgat tatacccgcc ttttgcctct agcagggtaa
     4321 ggccacgtcc aatctcagca cttaagcgaa ggcggatgtc ttccaaataa tgttcactca
     4381 tgaccataac agatttcttt cgattgatac ctgtttcgat ataagtcatg acaattgaag
     4441 tgattgcaat agataaaata gcataaagaa actcttctat tccaaaaata aacagattaa
     4501 gagaaacaac gaatgcgtct gtaaaaaata agccaatcga agtattcaag tggaaatatt
     4561 ttttaaaaat gaggggaggg atcgtcgtac cgccgctaga agagttgttt ttatataaaa
     4621 tcgcaactcc taaagcaaaa atcgcacttc caaaaatgac agacaacaac cgatctgaag
     4681 taatcattgt ttctggaact attgcaatga ttaaaggaaa aaccaagctt ccgaaaagta
     4741 ctttaaaaaa gggtttttta cctaaaaata gaagagctag taccaacata acgatattca
     4801 acactaaaat aactatagct cggtcaaagc ctaaagcagc ttccagtaat attcctagac
     4861 cgctgacacc tccagcagca atatgatgag gccctaaaaa catattgata gcgactccta
     4921 atatgaagag ggcaaaaata atcttaatgt atgtccatat aaattgtctc gtttctttc
//
LOCUS       DAJSKS010000165         4780 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJSKS010000165 DAJSKS010000000
VERSION     DAJSKS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.172"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(112..1260)
                     /gene="nagA"
                     /locus_tag="ON396_002558"
     CDS             complement(112..1260)
                     /gene="nagA"
                     /locus_tag="ON396_002558"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR3114600.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1336..2172)
                     /locus_tag="ON396_002559"
     CDS             complement(1336..2172)
                     /locus_tag="ON396_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3114601.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2356..3573)
                     /locus_tag="ON396_002560"
     CDS             complement(2356..3573)
                     /locus_tag="ON396_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR3114602.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3722..3901)
                     /locus_tag="ON396_002561"
     CDS             complement(3722..3901)
                     /locus_tag="ON396_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114603.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3956..4039
                     /locus_tag="ON396_002562"
     tRNA            3956..4039
                     /locus_tag="ON396_002562"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3990..3992,aa:Leu,seq:gag)
     gene            complement(4279..>4780)
                     /locus_tag="ON396_002563"
     CDS             complement(4279..>4780)
                     /locus_tag="ON396_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114604.1"
                     /translation="ILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
BASE COUNT         1449 a          997 c          772 g         1562 t
ORIGIN      
        1 aggaaattaa agaaactttt gaaacttatg taaagtgatt tttactagaa cacaaaaaaa
       61 accttaaaac caaacttaag aagtttagtt ttaaggttaa cttaaattat attatgcgtg
      121 gaaacgttcc acgccatcta gataagttgc ttccaatgtc atatcagggt tcaatacgat
      181 aaagtctgca tcgcgtcctt gagcgatttt tccacatgta tcatcgattt tacagctgat
      241 tgctggaatc agactagcca tcatgactgc ttgttctggt gttgctaatc cccagtctac
      301 aacattttta attgcttctt tcaatttcaa tatacttcca gctaagttgc cagattctag
      361 acgtgctgtt ccttctttta ctactactgg aaattcgcct aaattgtaat ttccatcagg
      421 catcccaccg gccatcatac agtctgtaat taatgcgaca tgatcatgtc ctgctttttc
      481 cattaggatt cccgcagcat ttggatgtac atgatggcca tcacaaatca attcagaaaa
      541 gacatgttct aaagataata acgcacccac catacctggt tcgcgatgat tcaatccacg
      601 cataccattg aaagcatgga caaatacact tgcgcctgct tcaacagctt tttgcgcttc
      661 ttctagtgta gcattactgt gtcctagaga aacaactact ccttcttcag tcacttgttt
      721 gacaaactct tctacacctt gacgttccgg tgctaaagca atttttttga ttaaaccacc
      781 tgaagcttct tgccattcat ggaaagtatc caaatcagga tccccgaaat aacttgggtt
      841 ttgtgcccct ttgtgttctt ctgtgaagaa aggaccttca aaatagattc ctttgatttt
      901 agctcctggt gcttcctgat acattttgcc gattgtctca gctacatctt ttaaacgttc
      961 tttgcttgac gtcaatgttg ttggtaaaaa tgaagtcact ccacaagata acagaccttc
     1021 agacatcact ttgatgcctt ctgcatcatt atccattaca tcatgattca tatagccgtg
     1081 aatatgtgta tctaccaaac caggagcaat ccatttgccg ctttggtcaa tgatgttgac
     1141 ttctccctca ggtagttctt tggtgtattc accaaaaagt ccatcggtaa tttcaagata
     1201 gcctgcatgt ttgacacctg acgctaaaaa gaatttgtca gcatagataa aagttctcat
     1261 tctattctct ccctttgttt ttctacctct aaagtactct tatttagaga agaagtcaag
     1321 aatggtctac accatttaca aattttttga aatttgtttc acagcttgtg ctaagccttc
     1381 atagaatgct gctctttctg gttcttcttt acgcttgtat attaagcttt taagcacaaa
     1441 taaatcattg aggtaatagt agctattctt ctcttgagcc caatagattc cttgattcac
     1501 ctgttctaac gcttcgtcga tttcattttg atggaataag aaatttccat aattataaaa
     1561 aatttgcgaa aattcacttt ttgatcgttc atcagctgat tgatgcagca gttggatgct
     1621 tcgactcaaa tagtagcgtg cttcagccgt ttttcctgcc acaccatata tttttccgat
     1681 acaactaatc agttgtattt ctgtgtcaga aacatggatt ctttcttttt ggctagagta
     1741 gcttaatgct tctttcaaat aataaaggga ctgagagata ttttgacgca acgtgaattc
     1801 gcaaacaccg agataataat aatagcgttg aaagtcagtt gcttgataca gattttcaga
     1861 aatatcagaa gattttagta cttgttctaa cttccgataa tcacgttttt ggaaacaact
     1921 atccaacagt tcaaataatt tatcgtttgt ccgagtagtt tttcgagaga gattcatcac
     1981 atgatcaatc gttaccccta gccgttgaca aatctgcatc atcaccaata cattcggcag
     2041 ttcctcatta ttttcgatcc gacttaatac actttgtgaa caaatatcat gcgaaagcat
     2101 tttttgcgtc attttttgtt cttttcgaat ctctttaatg atcgttccaa aggactcgat
     2161 taacgtttcc ataagtatcc tccccaaaat atgcgtattc gcattctatt cgtattttac
     2221 tttaggaagt cgcaaacttc aagaaaagac ttgcaactaa ataatatact ttagttattt
     2281 aagaaaactc cactagaaag agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa
     2341 atttggtttt taaaatcatt tttggcgata ggttgctagg aattctctca ttttcttagc
     2401 tgtttgtttc aaatcatcca ttttaggaag ataaacgatt cgaaaatgat ctggctggtt
     2461 ccaattaaat ccaccaccat gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac
     2521 gaatttttca tcatccaaaa tattgaatcg ctttgtatca atttttggga atatataaaa
     2581 agcagctttt ggttttacgg ctgaaagacc aggaatatca ttgattgcat tatagatata
     2641 ttctctttgt tcatagatcc ttccgcctgg caggagcagc tcatcgacac tttgataacc
     2701 gcctagcgcg gtttggataa tttgctgaga caatacattg gaacacaaac gcatggagga
     2761 caacatattc agcccttcta tatagccttt gacgtgtgac ttatctccac ttaggaccat
     2821 ccagcctact cgaaatcctg ctacacgatg agatttggat agaccgttca gcgtaacaac
     2881 gaacaaatca ggcgctaatg tagcaatcgg aatatgttct agaccatcca tcactaagcg
     2941 atcatagatt tcgtcggaat aaataatcag gttattttgt cgagctactt caacgatttc
     3001 ttctaataat tcttttggat aaagtgcccc agtaggatta ttaggattaa tgataacaat
     3061 agcttttgtc cgactcgtta tttttgattt gatatcatca atatcaggat accattctgc
     3121 ctcttcatcg caaatataat gaacaggtgt gcctccagct aatgcaatag aagccgtcca
     3181 caacggatag tccggcattg gaaccagtac ttcatctcca ttgtccagca acccctgcat
     3241 acacatcgtg atcaattcgc tcactccgtt gccggtgtag atatcattga tcgtcacatt
     3301 cggaaaatgt ttcagttggc aatattgctc aattgctttt ctagcagaaa agatcccctt
     3361 ggaatctgag tagccttctg agtcacgggc attcatgatc aaatcacgaa tgacttcgtt
     3421 tggtgcttca aatccaaaag gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc
     3481 ctcttcttgc atccgctccg cttcttctag aacgggaccg cgaacatcgt aactgacacc
     3541 ttctaatttg ttggattttt caaattctct catcacttag ccaatccctc atttcaaaaa
     3601 gttagaactt tccaaatttt ttgttattta ctatttcaat atcatactcc atcctagaaa
     3661 attttgccac ttatttagga aaaacgtaag tattaaaaaa acacaaacaa gcgttgagaa
     3721 cttaattgtc atgatagtca ttccatagat tcgctgccca atccattgca attccacaaa
     3781 tgattcctag cataaaacca agcaaaaaac gtccgaatgc aagtccaatc aatacaccta
     3841 agatcattcc cagtcctgaa aattctccgt catatttctc catcttaata agtcctccca
     3901 tttttgagct tgacaactat gtaaaacctc gctatcatta gtgtgttatt attacgcaga
     3961 tgtgttggaa ttggcagaca agcatgattg agggtcatgt gagcgttagc tcgtgtgggt
     4021 tcgagtccca tcatctgcac tacaactcgt gaagttaatt ttgcttcacg agtttttttg
     4081 tttgaacgtt tctttatgcc attatataca tatcactgta tcctttacaa aaagaacgta
     4141 caatcatgta aacagcctga ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa
     4201 cttgcaagtg ttaaaacaat taatcgaaaa acgagtaaaa tccctcacct aatactaaag
     4261 cgagggattt tttgtatatt atctgtttat caccgttatt ataaaggtac aaataccaag
     4321 cacggttaat ccaaattgca taaattcatt tttttgtaca ctcatgcttt tcattagctc
     4381 tttaactgcc attagtacta agatggcgat aacaacataa agtatatagt acgtccgcat
     4441 tccggaagcc catactgtgg gagaaaatcc cataattaac cttgaacaaa atcccattat
     4501 catcaaaata actaaaaaaa tagaagtttt cttattgtca aaacttaaat aaattccaac
     4561 aattaaacat agtaataatg ccaagactac taaagtagct atccaagtac ctggataaaa
     4621 aagtgacagt ttcgttccta actctgtaaa gagattattc aggttactac tactccaaat
     4681 cattgccctt ttatttccat tgacgtaagt aaagctttga cccattgtat taccaaaata
     4741 aataatcagg tttaagaaaa acggtaatgc agtcaatata
//
LOCUS       DAJSKS010000166         4757 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJSKS010000166 DAJSKS010000000
VERSION     DAJSKS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.173"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            51..746
                     /gene="vanR"
                     /locus_tag="ON396_002564"
     CDS             51..746
                     /gene="vanR"
                     /locus_tag="ON396_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCR3114605.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="ON396_002565"
     CDS             724..1878
                     /locus_tag="ON396_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCR3114606.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="ON396_002566"
     CDS             2093..3061
                     /locus_tag="ON396_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HCR3114607.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="ON396_002567"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="ON396_002567"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HCR3114608.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="ON396_002568"
     CDS             4091..4699
                     /locus_tag="ON396_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HCR3114609.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1471 a          910 c         1115 g         1261 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaacggttct gttgcaaagt
     4741 tttaaataaa gaataaa
//
LOCUS       DAJSKS010000167         4646 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJSKS010000167 DAJSKS010000000
VERSION     DAJSKS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.174"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(365..1156)
                     /gene="msrA"
                     /locus_tag="ON396_002569"
     CDS             complement(365..1156)
                     /gene="msrA"
                     /locus_tag="ON396_002569"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR3114610.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
     gene            complement(1347..1982)
                     /locus_tag="ON396_002570"
     CDS             complement(1347..1982)
                     /locus_tag="ON396_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCR3114611.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(2248..3300)
                     /locus_tag="ON396_002571"
     CDS             complement(2248..3300)
                     /locus_tag="ON396_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3114612.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(3313..4251)
                     /locus_tag="ON396_002572"
     CDS             complement(3313..4251)
                     /locus_tag="ON396_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR3114613.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT         1512 a          980 c          716 g         1438 t
ORIGIN      
        1 tagttattgt ttgcgctttt atacggaatt tagttcatca ttaatgtgga attcgattag
       61 gtgtctatta attatcaaga tgattctcta caaaattcca tagaaaaatc aagattctac
      121 aattcgaatt ctctttcaca tggaaataaa ggctagtaaa aaaacaggag ttgtgacaaa
      181 agacttgtca tacctcctgt ttccttgcga cttgaagctg aatgctttgc gccacaacct
      241 tttcctcggt gttctcaaag caactcctcg aaataattca taactgtcca aaaatatgaa
      301 aagctatttt tgtcagttgc tcattatttt gtcggagctg aacgctttgc gccacaacct
      361 tcctctatct atgtcgctga aaaaataaat gaagctgatg cgaatgcttg atctttttat
      421 atcttttagg ttgcttctga taaaattgtt gatgatgatt ttcagctggc caaaaagtct
      481 ttgcttggcg aatctctgta acgataggac ggctaaatcg gttagattga atcagttgtt
      541 ctcttgattt ttcagccaat tgcctttgct tttcattaac aacaaataga attgaacgat
      601 attgtttccc ccgatcttga aattgaccaa aggcatctgt agggtctgaa atttgccaat
      661 aaatctccaa cagctcttca taggttattt gacgtgtatc aaatatgatc tcaacagctt
      721 caacatgccc agtatatcca ccactgactt gatcgtaatt tggttttttg gtctctccac
      781 ctgtataacc agataacact gagattacac ctggttttgt ttcaaaaggc tcaaccatac
      841 accagaaaca gccaccggca aaaatcgccc gttcttggta taagggtgaa gttatcttat
      901 gtttggcata atcataagcc tttgcactcg ctgactcccc tgtgattctt aaataaaaat
      961 ccatcgcatc tggtgtcaaa ttatctcgta gtgctagcgg acgcaagttg acctctagtt
     1021 tttctatcac ctgctttatc gacatttttg ctaggtcttc ttttgcctct tcaaattgtg
     1081 ttctttccca ttctcttgta tttcgattta aaataatatt ataaaggtct atttccatct
     1141 gctccacatc taacatgaag tcaccctctt tcaagttttc ttcttattca ttcaaaaaat
     1201 gactacctga aatttagcac aacgcataac aagttaagac taacttgctt ttcgctctcc
     1261 tcatctctat tcttagtcga acacatccac acgaatggtt acccatagag aaattctttt
     1321 acttaatcag cttcacactg ctttttctac tttcgtagtt gaaaaaaact taaacaaatc
     1381 atcggcaaat agttaaagaa aaaacttaag cgttgaaaga agcccaattg cttaaagaag
     1441 gcaaactgac taatcgacgg aatccgtgct aatccgtaaa gacctgcaat tagtaaacta
     1501 ataactgtta aaacaagata aaaatggcta agcgtacctt gaccactttg acgataaagc
     1561 agaacaagta acaatgggaa aagtagaaag cctgcatagc ctagcccaga acctatatta
     1621 tggatccaag tcgatagatt ccaagagctt tcattcgtat tgatactgaa taacccagta
     1681 aaaatacaat cacctatccc atataaacca actgttgcaa cagcaagtat tgcaagtgtt
     1741 tttgatgttc caataaatag gtgatacaac gcaggcaacg aaagaacaaa aaaaagtcca
     1801 gatacaactg accagaccaa gaaagcacgt ctgactggac tatctacgtc accaaacaca
     1861 ctaattacgg tagtcatttg attcagtttg ggataaaaaa ggccaaggat atacggtgtc
     1921 gataaatcac taatcacacc taataataaa aaataaaaac catatttttg caaaaaaatc
     1981 attttcttct cctacttttt agtgattatt cgatacatcc aacaatcaaa ttttttctca
     2041 ttttaacaca tatttttatt ctagttgcct tttgaacaat caataacata aaggtgagtt
     2101 tcttctacat tttaaaaaat cgaatggcac tgaagagttc agttgtcagc atcaatctcc
     2161 tactcaaaaa ggcaaaaaaa cgtaagtcag atgttgttga catctcactt acgcatccat
     2221 tgattgaaaa tttcttagta aaatctacta gcctaaaaat ttgaagactt cttctcgatc
     2281 atacagttta aatactttct cgcataaaaa ttgagtaatt agtgccgcaa aaacagggat
     2341 gactaaccaa caaagaacaa caagaaagcc atttagccct gcatctaaag aagccaacgg
     2401 accaacaagt ccgaccacgc cgaaaccagc agaagcaggc gtacctgaaa tagaaaatag
     2461 agcgacagga atagctgtaa tcactgcact aactaaacaa ggaataagaa tcacaggttt
     2521 tctaaataga ttaggcatca tcatcttcat agcgcctaaa gcaatcgcaa ttgtcacacc
     2581 tgacttatta accttccaag agttcactac taaaacaaaa gtggttgcag ctacgcccat
     2641 cgcagccgca ccagcagaaa caccatctag ttggattgcc attccgattg ctactgttga
     2701 aataggagag ataatcaaaa aggcaaagga acaaccaatc aagatagaca ttaacagcgg
     2761 ttgtaaggta gtgaagttat taatcaatga tccaattgca gaagtaattg ctgttacaaa
     2821 agggaataaa taaaaaccaa gtaaacctgc acctacacct acaacaatcg gtgttaacac
     2881 aatcgacact gacccaaaac tatttccaat cagtaaaatc agcccgacag caatcgatgc
     2941 tgtcagcatc gtattaataa tatcgcctgt accagcacca acgtaaacac ccgtttcacc
     3001 caaatcaggt gtgaatttga ctacaccaga accaacaaaa gaagctccgg ctacaaccat
     3061 cattctcatc ggatcaaatc caaattgtaa ggcaatcaaa ccaccaatga tcaaaggtgt
     3121 tgctagttga aaaataactg ctaactgtgc gatcgttacc gcaaagggat acgcactaaa
     3181 atattttaaa atggcaccta atactgcatt aggaatcaat ccaataatga ttcctgttgc
     3241 tgtaccagcg aggactttat tcagaaaaat tttcggtgat attttttgtg aagtttccat
     3301 tcaatctctc ctttatttta cgttcaaact atcttctttt gcatggacaa gctcagtcaa
     3361 gaaatcacaa taaggcgtcg caacaccata tttcttccct tttctagaaa ttgcgccatt
     3421 gatataatcg atttctgtta atcgatggtt cttaatcaag tcttgataca ttgaaggata
     3481 atgtaaccca attgtttccg ggtcaaaaca agattcacaa tgtgcaatga cttctgggac
     3541 atctagttca atcttctcta ctgctgctac tttggcaaac tcattgacaa tcgtcgccac
     3601 cattttatga gcagtcgatg tttttcctag ctcagccata ttaacgtcta agattgtgca
     3661 tagtccgttc attgttccgt tcacacatgc tttgcgataa atagaatagt gaatattgtc
     3721 agaaaaatga gcatttaatc ctgattcaga caatttgtct gctaattttt ttgcagctgc
     3781 ttcttttcca tcacctaaat tttgtaattc tactgaacca cttccaaata atttgacctg
     3841 accaggacct tcaagacctg ccgtccacat ggtatttcca atatagatat tttccattgg
     3901 tacaaatttc tcaataatat cttcatgccc gataccattt aatagacata agacctctgt
     3961 atcttttttg attaatgatt ggatatcctg cagcattttt tccagctgca tcgctttggt
     4021 aaatagaata atcagatcaa cttgatcttc tttttctact tcggattgaa gaacgattgg
     4081 tagttttgct tctacctctt taccattaaa atttgcttgt aatccatgct ctttgatttg
     4141 ttggacatgt tctgcccagc catcgattaa taatacttcg ttcccacttt ggtgaagcat
     4201 taacccaaag cgacttccca ttgcgccagc gcctgcaatt gctattttca ttgattcttc
     4261 ctcctttttc tttctattat attcatttga gaatcatttc acaaccttgt tttgttattc
     4321 ttctcttatt tatcatgttt atatccacca ttcaagttat tttatcagca aatcaataaa
     4381 aactggaata gaatcccaat ttcatcgtaa gagaaactag actagcaata agaaaataga
     4441 aacattcata gaaaaaacct cactcctctg attataaaag agggggcatt aacctcgtgt
     4501 atactaaaac cttccattca ttcgatggat gattttcttt ttgtgaaaaa atattcattg
     4561 tagtacgatg ttgcgcaaca cagacggatg gtattgctta ttatttatta aaatcgaaca
     4621 atacacttat ttaaaaactt ctttcc
//
LOCUS       DAJSKS010000168         4593 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJSKS010000168 DAJSKS010000000
VERSION     DAJSKS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.175"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            189..2858
                     /locus_tag="ON396_002573"
     CDS             189..2858
                     /locus_tag="ON396_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HCR3114614.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2949..3569)
                     /locus_tag="ON396_002574"
     CDS             complement(2949..3569)
                     /locus_tag="ON396_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3114615.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3683..4222
                     /locus_tag="ON396_002575"
     CDS             3683..4222
                     /locus_tag="ON396_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114616.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4219..4557
                     /locus_tag="ON396_002576"
     CDS             4219..4557
                     /locus_tag="ON396_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HCR3114617.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1423 a          790 c          860 g         1520 t
ORIGIN      
        1 aaaattttta agggtgggac ttttgattcc tacccttttt ttgcgtttat ttttgttata
       61 cgaacaaatt atgaagttat ttattctaaa ttttattttt ttgtgaaaat tagactcttt
      121 ttaagcagag aaaaacaagg atatgataaa ctgaatacaa cttcatagaa atttggtgga
      181 aaataaatat gaaaaaaaat ttaaagtcat tctttagaga aaacggacgc tacgcagcag
      241 ctagtttcct gattccgttt ttgattttag cacttgttta tctaagtatc gggatctatc
      301 ctggaagcag cagaagtatt ttggcaagtg atgccttttc acagttttct aattttcatg
      361 ccagcttccg aaatgttctt cttggaaagc aaagtatttt ttatacatgg aatgcttctc
      421 taggattgaa ttatttatct ttaatttctt actatctagg cgggatcttc acgccactgg
      481 tgttcttttt tccaaaccag atgatgccag atgcactgta tgttctgact ttgcttaagg
      541 tcggctgtgc cggattggct ttttggtttc ttgcacgtac gttcaagatt cctcgctggg
      601 ggcatgttgc acttagcgtc agttacgcat tgatctcatt tatcactgca cattcggaga
      661 tcatcatgtg gctggatgca tttgtttatc ttccattagt gattttgggg attcatcgtg
      721 ttatggatct taaacggccg aaacttttat ttatcagtta tttactcttg tttttatcca
      781 gtttttatat gggttttatg attggcgtat tttctttctt atattttatc gcccgtctat
      841 tgacaaactg gtcagaatat aaaaaaacga ttgttcctta tggtatcact tctttactgg
      901 caggcggcgc ttcaatgatc attgtattgc cagcagtgct ggatcttcgg tcaaatggcg
      961 aggcactgag tcagatcact acgttgaaaa cagaagcgac ttcttatctg gatctaatca
     1021 tgaaaaatat gattggtgtt tatgacacga caaaatacgg atcgattccg ttcgtttacg
     1081 caggtctgct accattagtc ctctgtctgt tttattttgc cagtcggaaa gtaccgttga
     1141 aaaataaatt tttgtacggc agtcttttcg ccatacttat cgcaagtttc tatcttgttc
     1201 cattgaattt gttctggcat ggtatgcatg caccgaatat gtttttattc cgttatgcct
     1261 atctgttctc tttcttagtg ctcctgcttg caggaaaagg atgggaaaag atagaacaag
     1321 acgatcgtgg gctgttgtta ggaatcattt tggtcgtagc tgctctcttt gcggtggcat
     1381 ggggcatcaa acctgaagga agctacacct atgtgacgat gacttccttt gtattgacag
     1441 tggttttctt agggatttat gcttttgctg tgggatttta tcagcataaa aaactttcat
     1501 tcaatcatct gagtatattg ttattattga tcatgtctgc tgaagcactg gtcaatacga
     1561 acagtatgat ccatggcatt cttgatgatt ggaattatgc ttcccgcagt ttatatactg
     1621 atccttatcc tgacttgaaa gctcttgtaa atgaaacaaa aaaagattcc gactcgtttt
     1681 accgcttgga aaatctggat ccagtatctg caaacgattc gatcaactat ggttacagtg
     1741 gtatcagttt gttttcttct ataagaaatc gtaattcttc ctcttacctg gataaattag
     1801 gtttccgttc aagagggaca aatttgaata tccgttataa caataatact ttgctaatgg
     1861 atgggtttac aggaatcaaa tataacattt ctaaaaacga aagcacgttc agtaaatatg
     1921 gcttccgcca agctggtcaa agtggcgact atacactgta tcagaatgtc aatgcattgc
     1981 cactagcatt tactgcaccg ttgtccgtta atgatgttga gcagccagtg acagataatt
     2041 tgaccagtca gacaaacttg atcaatacat tgtctggttt aaatgaacag tattttacat
     2101 tctatacacc tactttgaag aatcagttga atacgacgat cacaaagaca acgactggcg
     2161 tcacttactc agaaacagca gataaccagc caaaagagct cacttgggaa gtccatgtgc
     2221 ctgcaaatac acaagcttac ttaagtttgt tcccaaccaa ttttgcacaa ttagaaagtt
     2281 ctagtgcgac gatcactgtc aatggtacat cgcgaaaaac gcaaatcaac atttctggtc
     2341 aatactatga tcttggctat tatcctcaag agacaacagt gacatttact gcaagttttt
     2401 atggaacgaa agaagtaagt ttcatggagc ctaaagtcgt tggtctagat gttgtagcct
     2461 atcaagccgc aatgaatcag atcaaagaaa aaggtgtcga aatggaagcc actggcagaa
     2521 aagcttctgg gactgtaaat accgctgaag attccatgtt ggtgacaact attccttatg
     2581 acaaaggatg gaaagctaaa attgatggga aaccagtcaa ggtcagcaat ttcaaagacg
     2641 cattcttgat gataaaagtt ccagcaggta aacatactgt cactttcagt tacttgcctg
     2701 aaggatttac gataggtgca atcctttttg tcctatgtat tgcattgttt attctttatg
     2761 tctggtacct tgaccgcaat cgcccctttt atggcacgat tgatccagca atagaagaaa
     2821 aaccgcgaaa tacacacaga agaagacgaa gaaaataaag aaacgatgga atctgtgtca
     2881 ctcttctatt taaatggcaa aaatttcaat ctttaaatga caaaaaccac ctgatttttg
     2941 gtggtttttt atatacctat gactaaacta tctcttgctg cttcgacaac ttctacagtg
     3001 atcttatcca tttgattgat tcttaaaatc cgatcaatct gcgcaaataa acgatgaata
     3061 agcctaaaat ttccttttgt gatcttaatg atagttgtga tagcctcata atcagaaaaa
     3121 tcttctagct tgatatcaat tcctaaatct gcccatttgt attccaaaat atgatgtgtt
     3181 tcgtccttac taaggttatc aaattcatgt gcaaatccta tgcgagagta aagctgcgga
     3241 taacgagaca atcttttttc aataccaggc ataccgataa gaaccattgc cagatcattt
     3301 ctatcgtaaa tatctcttag ctgttctaaa tgctgaagct ttaaacgatc aatttcatcg
     3361 acaataatta agtcaattgc ttcataggtt ttaggcacag gaggatcttc ttgattgtat
     3421 tttagtacca tatgcattgc tttagtcaga cctattctac tagttattgt gttaatatcc
     3481 gaactgacac gagacatctt ttcagctggt acagtataaa aaactttatt tacacttaat
     3541 atttcatctg ttgcatttat caagaacaag aaaaagtctg aatattcaaa aaaaataacg
     3601 gtgtacattt ttaaaaggag atgtataatc gagatttaga aagcgttttc ctcaaaattt
     3661 aatgaattag gaagtgatta ctatgaaaaa attatttagc gcacttttta ttctgttatt
     3721 tggctttatt ggtattggga cttctggaaa tatagttaaa gctagcacga tggttgaaca
     3781 aacaagtgaa atggcaaaaa cttttagtca attaacacca gaagaacaaa gttacttcaa
     3841 atccaaagga tttagtgata aagatacttt ttacactcaa aagatagagc ctcaagatcc
     3901 gaaaacacgt ttttctgcaa atgtattgcg gttaaccgca tctactaaaa aaattactaa
     3961 tacgcagggg cggacagaat atattataac aagtcccaat tcggcgatgt atcgtatttc
     4021 tacacgcttg agtttaggta gtgcaaaatc atatactcaa acagtaaatc tagctaattc
     4081 ttatggttat ggtggaggaa tgtattttac atttactggg aaaagacaat attacagtgt
     4141 aaaattagct atgcaagttt ataatacatt tggtgtaggg tcagtctctt gcactgcggg
     4201 aggattgaca ataggaaaat gataatttct tactatgtct taaatagaag tgatgatgaa
     4261 ggactcttcg tttttcaatc attacatgag gatacgatgc taaaagagtt ttataatcgg
     4321 attaagaata aacagataga aacaaattta tattttttaa gtgactctat cttgcctttt
     4381 attagcaacc ctaaaattct taacttgttg cagacaaaag atttgaaaag tttaccgatt
     4441 attgtcattg atgaggaaat tgtaaaatat ggcgcttatc taagcgtaga agaattagaa
     4501 aaattaacta acattggaat gagtattcag gaggataatg gggtacatgg aacatagaat
     4561 gaatatgcac agttgagaac ggaatttgac att
//
LOCUS       DAJSKS010000169         4585 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJSKS010000169 DAJSKS010000000
VERSION     DAJSKS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.176"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(12..482)
                     /locus_tag="ON396_002577"
     CDS             complement(12..482)
                     /locus_tag="ON396_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCR3114618.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="ON396_002578"
     CDS             complement(641..1099)
                     /locus_tag="ON396_002578"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR3114619.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="ON396_002579"
     CDS             complement(1270..2415)
                     /locus_tag="ON396_002579"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR3114620.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="ON396_002580"
     CDS             complement(2428..3345)
                     /locus_tag="ON396_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCR3114621.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="ON396_002581"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="ON396_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR3114622.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1431 a          841 c          869 g         1444 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaagc
//
LOCUS       DAJSKS010000170         4498 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJSKS010000170 DAJSKS010000000
VERSION     DAJSKS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.177"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..1970
                     /gene="pglX"
                     /locus_tag="ON396_002582"
     CDS             <1..1970
                     /gene="pglX"
                     /locus_tag="ON396_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HCR3114623.1"
                     /translation="YASFMTPFVWMFISSYEKLRSFLINERNISSLIQMEYSAFEEAT
                     VPICTFTIKNSNEQDGSYIKLSDFKGGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKK
                     IPGMPIAYWASDNVITDFAQGKLTNDVADAKQGLATGNNNKFLRYWFEVEFNKIKFDA
                     KTLEDAKKSRKKWFPTTKGGRFRKWYGNNEYVVNWENDGDEIKNYVNENGKLKSRPQN
                     LNYYFKKGITWSTLSSGQVSFRLQNQAIFETKGSVLFSKDITDFEEIFGYVNSKLVNH
                     FLTFISPTLDYHEGPMGKLPFLKVTSERKLKKIREKVDENIDIARKDYNALETSYEFR
                     RNILLSYMFKKDLSEAFNCLSRQLVNQFNTLKSNEEELNDIFINHYSLEKELTKEVQD
                     EYISARLPDKAREIKDFISYSIGCIFGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNV
                     LLITDDEYFKDSQIDIVNRFIDFVEITFGKETLEENLQFIADTLGGKGTSREIIRRYF
                     VQDFYKDHLKTYSNRPIYWQLDSGKQNGFKALMYLHRYTPDQLGKVRTEYLHELQKAY
                     DNRIELRQQQISTSDNPKERNAMTKEIVKIQKQLKEVREYDEKLGHLALKRIELDLDD
                     GVIVNYDKLQRDPKTGEKFQIFAKGVKEPKK"
     gene            2086..>4498
                     /gene="pglZ"
                     /locus_tag="ON396_002583"
     CDS             2086..>4498
                     /gene="pglZ"
                     /locus_tag="ON396_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HCR3114624.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYT"
BASE COUNT         1639 a          626 c          854 g         1379 t
ORIGIN      
        1 gatatgcttc ctttatgacg ccatttgttt ggatgttcat ttctagctat gaaaagttac
       61 gctcattttt aattaatgaa agaaacattt ctagtttgat tcaaatggag tattcagctt
      121 ttgaagaagc aaccgttcca atttgtacgt ttacgataaa aaattcaaat gaacaagatg
      181 gaagctatat aaaattatca gattttaaag gtggaatgga agttcaaaaa attaaaacgt
      241 tagaagcaat tcatcagcat ggtgaatgcg attattttta tagtactaat caagacaact
      301 tcaaaaaaat cccaggaatg ccgattgctt actgggcaag cgataacgta atcactgatt
      361 ttgctcaagg aaaattaact aacgatgttg cagatgcaaa acaagggttg gcaaccggaa
      421 ataacaataa atttttgcgg tattggtttg aagtagaatt taacaaaata aagtttgatg
      481 ctaaaacatt agaggacgct aaaaagtcta gaaaaaaatg gtttcctaca actaaaggtg
      541 gtcgttttag aaaatggtat ggaaacaatg aatacgtggt taactgggag aatgatggag
      601 atgaaataaa aaattatgtt aatgaaaatg ggaagttaaa atctagacca caaaatctta
      661 attactattt taaaaaggga attacttggt cgacactttc atctggacaa gtatctttta
      721 gattgcagaa tcaggcaatt tttgaaacta aaggatctgt tctgttctca aaggatataa
      781 ctgactttga agaaatattt ggctatgtaa actctaaatt agttaatcac tttttaactt
      841 ttatttctcc caccttggat taccatgaag gaccaatggg aaaactacct tttcttaaag
      901 ttacatctga gagaaaatta aaaaaaataa gagaaaaagt ggatgaaaat atcgatatag
      961 ctagaaaaga ctataatgct cttgaaacgt catacgagtt tagaagaaac attttgttaa
     1021 gttatatgtt taaaaaagat ctctcagagg catttaactg tctaagtagg cagttggtta
     1081 atcaatttaa tactctaaaa tcgaatgaag aggaactgaa cgacatattc attaatcatt
     1141 attctcttga aaaagaatta acaaaagagg ttcaagatga atatatctct gcaaggttac
     1201 ctgataaggc gagggaaatt aaggatttta tttcttattc aataggttgt atcttcggtc
     1261 gttactccct agacgaagat ggtctcatct tcgctggcgg cgaatgggat ccttcccgct
     1321 atcaaaaatt taaacctgat actgataatg ttttactcat caccgatgat gagtatttca
     1381 aggatagcca aattgacatt gtcaatcgtt tcattgattt cgttgagatt acttttggta
     1441 aagaaaccct tgaagaaaat ctccagttta tcgctgacac tttaggtggc aaagggacaa
     1501 gtcgagaaat tattcgtcgt tacttcgtac aagattttta caaagaccat ttaaaaacat
     1561 actccaatcg accaatttat tggcaattgg actcaggaaa acaaaatggc tttaaagcgt
     1621 taatgtactt gcatcgctat acaccagatc aattaggaaa agtacgtact gaatacctac
     1681 acgaactaca aaaagcgtat gacaatcgaa tcgaattgcg tcagcaacaa atttcaacaa
     1741 gtgataaccc aaaagaaaga aatgcaatga caaaagaaat tgtaaagatt cagaagcaat
     1801 taaaagaagt tcgtgagtat gacgagaagt tgggtcactt agcattaaaa cgaattgaat
     1861 tagacttaga tgatggtgta attgtcaatt atgataagtt gcagcgtgat cctaaaaccg
     1921 gcgagaaatt ccaaattttt gcaaaagggg tcaaagaacc aaaaaaatag ttatctaaga
     1981 aagttgtttt ctaacaactt tcttttttat tcttaaattg cgtgcgaaat tatccaaatt
     2041 atgtcaatat aaggggtata gaaagagaac gattaggtga gattgatgac agatataaat
     2101 acgcaacaaa ttgaagaaaa attaaatcaa cgttttccta attatggatg taggaaaatc
     2161 attttttggt ttgattcaaa tcaggatttt cttgaagaaa ttgactcatt ggtcattcaa
     2221 aatgcaaaaa tctatcgatt agaaaaggac gcacaattta aaactaagcg tttattggaa
     2281 tatgaagatc ccgaaactaa ctatttaatc tatgcgccat ttcctcgttg ggatgataaa
     2341 gatgaagata atcatttgtt gagtgtattg aattattcag aggaatttag tgcagaccgc
     2401 ttagcgatta tcatgaatga attggctatt ccacttagat ttcatcagac agttaaacag
     2461 tatcgtaatt tctttaatgc aaaggatcgc atgagaagtt ttggtgaatt tagtacgcat
     2521 atgacaatta ataaatcaga aacgattgaa ttaatgataa tggctgtttt gttgaaatcg
     2581 aaaagtattc gattaaatga tttactgcgt gtagttctaa aaagctttac agaaagtagg
     2641 gagaatcctt taagggaact tgataagtat cagttaacag ctaaattttg ggaatttatt
     2701 caaatgaaat ttggctttac ggatagccag ccaacattgg aaaagctgac gatgtgtctt
     2761 tatttgaact atttttatta tcaaattgat caagagatgc cgaagaagtt caaaggattt
     2821 gaaatactac atttgaaagc aaacattatt actttcatgg atcagtttat gaatgataca
     2881 cgttacatgg aaagctttga acagctatct aaaaaagtat atcaggctat tgatggtgat
     2941 ggattattag acagtataga aattgatgat ctaattgaag cagacgtatt ttctgagatt
     3001 catgcaaaaa tattaatgtt ttatgcagaa cgattaaagg cttctgacac tacaacgaaa
     3061 attgcaggac aaacaatttt tgatagttta aagcagcgcg aaaaaatgca tttcgcatcg
     3121 gattatattt atgagtatca attattgaaa catgcttatt atcttgtgga taaaaacaga
     3181 ttgtgcttgc cgatgaacat ggataatttg ctaactcaat atgagaagga aatgtattgg
     3241 gttgatacgc attatcggaa atttatttgg aatagtgatc atatagaaga aaagcaaaaa
     3301 tatggccaat tgtatcgatt agttgaagca aattattcga agtttcttga tcaatcagga
     3361 caaatttgga atcaaacttt tgaatttaag aatcgtccat ccattcgaaa attttataat
     3421 caagctgtaa tgaaacgctc tgttaaaact gtagttatta tttcagatgc ttttcgatat
     3481 gagttaggga tgaatttaga acgtaagtta gttagagaaa agaagtattc aacaaacatg
     3541 gctagctttc tttctgtatt accttcagtt actgaattcg gtaaagccgc cttattacca
     3601 aacaatcaac taacttacaa tgaaggaaca gaagtactga ttgatgggaa aaaaacgaat
     3661 ggtttatcca atcgaaatgc gattctccag caacgagatg caaatgctgt agctttaaat
     3721 tatgatgatg tggttaagat gaataaggct gaggtacgag aagtatttaa tggcaaatca
     3781 gtcatttata tttatcacga tcaagtggat agaacaggtg atcactcgaa tgaaaatcga
     3841 gtttttgagg cctgtgattt agcgataagc gaaatttccc gacttattaa tcgcttgcat
     3901 aatgatggca gtatctatcg ttttatagtt acggctgatc atggatttat ttataaaaga
     3961 agccacgttg aagaagtaga taagattgaa aatccatcta gaaatgaaaa agatcgtgta
     4021 gaacgtcgtt ttattcttag tgaagaagaa tacgaaataa ttggtacgaa aaattactct
     4081 ttgggagatt ctttaggaaa tgatgacact agaaacattt gtgtcccgat tacttcaagt
     4141 atttttaaga aagctggcgg gggacaaagc tacgttcatg gagggtcatc tattcaagaa
     4201 atgctagtcc ctgttctaga aattacggta gccaaaggta gcaatgcaaa ggaaccagta
     4261 gcaattgaat taatgactag caaccgcaga attacaggat tatcaaccac gttagaattc
     4321 tatcaaaaaa atgcaattag tgatgtttat gaccccgcat ctttctggct ttattttgaa
     4381 gatgaatatg gtgaacgtat ctcaaatgaa gaaatctatg ttgcggattc aaaaacagaa
     4441 gatgcaaatc agcggtttac taaatttgtt tttgaatttg taaatcgtcg atatacag
//
LOCUS       DAJSKS010000171         4425 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJSKS010000171 DAJSKS010000000
VERSION     DAJSKS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.178"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            690..1658
                     /locus_tag="ON396_002584"
     CDS             690..1658
                     /locus_tag="ON396_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3114625.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="ON396_002585"
     CDS             1669..2484
                     /locus_tag="ON396_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR3114626.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ON396_002586"
     CDS             2552..3262
                     /locus_tag="ON396_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3114627.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ON396_002587"
     CDS             3298..>4425
                     /locus_tag="ON396_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR3114628.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAJSKS010000172         4364 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJSKS010000172 DAJSKS010000000
VERSION     DAJSKS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.179"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(38..934)
                     /locus_tag="ON396_002588"
     CDS             complement(38..934)
                     /locus_tag="ON396_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3114629.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(927..1607)
                     /locus_tag="ON396_002589"
     CDS             complement(927..1607)
                     /locus_tag="ON396_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3114630.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1608..2390)
                     /locus_tag="ON396_002590"
     CDS             complement(1608..2390)
                     /locus_tag="ON396_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3114631.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2383..3300)
                     /locus_tag="ON396_002591"
     CDS             complement(2383..3300)
                     /locus_tag="ON396_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR3114632.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3464..3787)
                     /locus_tag="ON396_002592"
     CDS             complement(3464..3787)
                     /locus_tag="ON396_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114633.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         1280 a          951 c          760 g         1373 t
ORIGIN      
        1 atacattttt ctactagttc tcactaatct tgtataatta aaccctccgc aacttgacgg
       61 agatggagaa gatgcccccg tctaatctag cggtcatctc tcctcccatt ccttccacca
      121 atttttttga caggtaaagc ccgattccgc tagattccgt atttgtgcgg ctggtatttt
      181 cggtataaaa acgctggaag actttttcaa cagaggtttg cgttcctggt ttgataggat
      241 tggccactgt taatcgaatg aatggatcct cttttgaaaa tgtcaccttt gcttgtccat
      301 ctgcataacg caataaattc tgtatgatat tttgcagcac tcgcagcaac atgagcggat
      361 ctgctaatac agttgtattt atcgagtgtt cagcgaacgc tacctcgata tttttctcag
      421 aaaattgttc ataaaaagag aaaaatactt cttccacctc tttagaaaaa gaaagcgtat
      481 ggagatccat ttctttttgc ttcgtctcta ttcgagcaag atcataaaac tgatccgtca
      541 tactgagtaa tcttgcaaca gattgtttga tgatcgttaa atacttctcc cgtttctccg
      601 tatcctctac ctgttccagt aattgtacat aaccgttaat agcagtcaaa ggagttctga
      661 gatcatgaga aaggccgacg atcgatagtc tgatgttttc ttccatttct tgaaatgctt
      721 gtcctttttg ttcattttca tccaaccatg tattcagtgc atctactagg tccattagat
      781 tcttttctcg aaattcgact gaaagacgac caccatattc ttgtcgttca gaaagagtgg
      841 cgattttctt agttaatctc ttgatttccc tttgcaggaa gaaaataaaa cttcccataa
      901 agataacaac gagaagcaac agccatttaa tcaaatctaa accccactcc ccacaccgta
      961 tgaatataca cagcatgtgc ctttgctagc ttccctcgaa gattggaaat atgcatattc
     1021 actgttttct cactatcaaa atatggttct tgccaaactg tttcataaag atttgctcgc
     1081 gaaaatactt ttttcggatg ttctaaaaaa agcttcagca gatcgaattc tcttcctgtc
     1141 aaatggacag ccacaccatt cacatttaca tccctcgact ctttgtccag taagatttcc
     1201 ttgtaataga tgcgttgcga cagatctttt acttggacag aagtctgatg acgaagttgg
     1261 atgccgattc gtaacaaaag ttcttcgata tcaaatggtt tcgtcacata gtcatccgct
     1321 cctgctgcca acaaggaaac tttatctctt tgatcgcttt ttgctgaaag aacgatgatc
     1381 ggaacattcg atgtctgtcg gatttctgct aacaatgctt cacctgaaag ccctggaagc
     1441 atgagatcta gaaggatcaa atcgacagga tatgtgctta acaataattt tccttccgta
     1501 cctgaataag catcaagaag tttgtatctc tgttcaagaa cctcctccag taaggaatga
     1561 acagcttcat cgtcttcgat aatcaaaata gtttccagca ggaccaccta cttaatgtca
     1621 gatttttgga aaataaacat tcctagagct gatgatacca cgattgttac tagactgacc
     1681 agaatcattt tcggtgcttg cgaataagtt gcttgttgcg caaagtccgt atacatgagc
     1741 cacgtttccg taaaccactc cgttagaaat tccaagcctt tgatttttgc gaaaacaccg
     1801 ggaatgaagg ccgttcccat gatataaaga aaagaaagaa gcaaactgac actggtactt
     1861 ctagtgatga atgtcaaagc cactggaaca gataatgtcc ctaatgtcag cagaagcaat
     1921 acaggaaata cttttagaac ataatttcca tagtttttga ttccctcaac ttcaccaaat
     1981 ccgaaagcaa gtgtgtaagc aagcatggct agtcccgtga tcaaaaccac gccaagtgtt
     2041 gtgatcaaaa gcgtaacgag aagtttcgac acataataag tcattcgatt ccgtcctaaa
     2101 gataacgtat tgcgaatcgt accgttttga aattcttccc caatataaaa gcacgcaaat
     2161 gctgcgaaag ccagaagaat ccatgcagcc attttttcta ccattaaaat ccctgttgca
     2221 ccgttgatga ctgtatccgt tgcttggttt aattcattgc cgtattttgt atgcatatac
     2281 cattcgttcc aagaataaaa aagcggaagt acgaccatca gtcctagtga gacccacatt
     2341 ttacggctct tccatacttt tattttttca gcagttaata gattaaacat gtggattgcc
     2401 tccagttaat tgtaagaaat aatcttccag tttttgccca actaggtgaa taccatccac
     2461 aggaactttt gcttctacca atgcttgatt gatggctgct acttggtcaa ttccttcaaa
     2521 aagctggata acacggctag attgtacaaa ataatcccta taaccaagag agtcgagaac
     2581 cgttactgct tgttccgtcg ccgaagtgtc aatttttata aattgacgag attcttgtgc
     2641 caattcttct ttcgataatt ctttgatcaa gtgtccttca tgcaaaatac catagtgagt
     2701 ggcgatctgt gacaattcat ctagcaagtg gctagaaagc aagacggtga tgccccgttc
     2761 ttttaccaaa cgttggatga tttctcgcat ttctgccatc cctgctggat ccatcccatt
     2821 cgtcggttca tccaaaatca aaaattctgg attcgtgatc agtgcatttg caatagctaa
     2881 acgctggcgc atacctaaag agaaattctt tgctttcttt ttccctgtat tttccaatcg
     2941 catcattttc agcaggtctt cgatttctct atccgacaca ccgccgttcg ctgcttggat
     3001 acgtaggtta tctcttgcag ttaattctgg ataaagagca ggggtttcaa tcgattgacc
     3061 catacgtcgt cttgctgcct gcaaatccgt cgttccaaat aattcgatcg aaccttcatc
     3121 aatcgagatc aaacccataa tagttctcat aaaggtagac ttacccgctc cattttcccc
     3181 aatcaatcca tagatcattc cacgtttgat ttcgattgat actttatcta atgctttggc
     3241 agcaccgtat tttttggtta taccagttgc tttcaagacc gtttcatttc caaaatccat
     3301 acttatcctt ctttcttatt gatgagtgaa gtatagttga cgggtataaa gaaagcagcc
     3361 agaaatgtat aaagaaagtg taaattttcc aattgaatgt caaaaaaaac aatcggacaa
     3421 tacgtgtctt tcctattatc aggcagtttg ttatacgctt gtatcagttt cctgattctt
     3481 tcttttcgac caaagtcgtt tctctgtatt cctcttcact gaaaattttt gcttctttca
     3541 gatcaagctt gagtaatttt accagaccgg ttataaggat aactatagca agactccctc
     3601 ccagaaaaat tagactgtct cgcagacgtg gttgcgttgt cttccaatca aagagcgcac
     3661 caaacagtgc taatccaagt ggactggcca ccatggtaat cgttgttatc gttgacataa
     3721 gtcgtccttg tatatgcgtt ggaacttctc gctgaagcca gaccatataa aaagcaaaag
     3781 aaaacattaa tccttaaaaa cggacttatg aaaaataata catgccgttt gggatttaca
     3841 attagctacc aaataagagg ttatgacaaa agacttttca caatcccttc ttccgaataa
     3901 acggtgttct cctgccaact cctcggtcac aagtcacaat atttatcaaa catgagaagc
     3961 aatttttgta aattccttct tatttcttga agctgaccac ttctgtcaca acctcagaag
     4021 cggataaata atattgtttg atgatacttt atctttttcc cgtttgttta tagatcgaat
     4081 gatattatag accaagaatc ctacctatta acatagctaa aaacacaaaa aaggcacttt
     4141 ccaaattgtg gaaagtgcca gaaaagttag gattaaggta actgcgctca gcattgcttc
     4201 gcccagtacg attctcgtaa ctcgtgaact cgaacgataa tcgcaattaa cgttttgaga
     4261 actgtgaagc tttacgggct ttcttaagac ctggtttttt cttcgtatca ttgaataagt
     4321 ttgaaaaacc ttgatttata aaggttttcc cgtaaatata tttt
//
LOCUS       DAJSKS010000173         4266 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJSKS010000173 DAJSKS010000000
VERSION     DAJSKS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.180"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..265)
                     /locus_tag="ON396_002593"
     CDS             complement(<1..265)
                     /locus_tag="ON396_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR3114634.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYE"
     gene            complement(288..1688)
                     /locus_tag="ON396_002594"
     CDS             complement(288..1688)
                     /locus_tag="ON396_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HCR3114635.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(1692..2102)
                     /locus_tag="ON396_002595"
     CDS             complement(1692..2102)
                     /locus_tag="ON396_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR3114636.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2120..2611)
                     /locus_tag="ON396_002596"
     CDS             complement(2120..2611)
                     /locus_tag="ON396_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR3114637.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(2621..3412)
                     /locus_tag="ON396_002597"
     CDS             complement(2621..3412)
                     /locus_tag="ON396_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR3114638.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3405..4178)
                     /locus_tag="ON396_002598"
     CDS             complement(3405..4178)
                     /locus_tag="ON396_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3114639.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1326 a          898 c          565 g         1477 t
ORIGIN      
        1 attcatatcc taaatcttga attgaattga atacctcatc tatgtcatcc gtatctaatg
       61 ctagatgatc aatggctcct ctacactcag tagttttatc ttcccatatt tctattatcg
      121 ttccattcaa ctcgaaaaag ccgactttac ttcccacatt catttcttca ttaatcagac
      181 taaaccctaa ggattcataa aagatttttg aagctccaaa atcttttgtt ggaattccaa
      241 tatgttgtaa accatttaat ttcacttaca tcatcccttc aattgtttta aaatccacat
      301 tcataattta ttgaatagtc tagtttatct accttgattg ctccctccgt aacagtcata
      361 cttaatgctg ttccgacttt atcatacatt ctagtactta aagcaacatt atcaatatat
      421 aaaacagcta tcgtatcatc aacaattaac tttagttcat attctttgcc tatttccaaa
      481 aatagtggtc gttgcagccc cttatcaaac atttgaggcc aaggatagtt cggagttttt
      541 ccgaaagtaa gctgattatt aatcaatgaa atacgaaatt cgtatccttc ttgagtatca
      601 ctatctacaa ataattttaa agcaaactcc tttgttaacg atgtaatagt caaagttgtt
      661 tcaaatagaa aataatcttc gattttagga acaattactc tttctttttt tccaaattct
      721 ttctctaact taaaacttga aaatggagtg atttctttaa aattctcaat tattttttca
      781 ggcagtttaa caccaagagt tttgtctgtc ttttgaataa tttgatgtgg aacaagtgca
      841 ccgccccaat cccagttatt ttgatcgttc tccccttctt ttgttgccac ccagccagtt
      901 agaaaacggc cagtttcatt agttccgact gttcttgctg cataataggc ttttccgtcg
      961 aaagcttcgt ctactggtga tttccaatct gaaaataatc ctgctccgac tcgatattta
     1021 gtctttttat cttcacaata ctctgaaaaa agtaaatacc acgttccatc aatttcaaat
     1081 atatcaggca tttcaatcat gttaaattca ttattagccc agtagtctcc tttgaaattc
     1141 caatctttta aatcttcgga ttcaaaatag accaatctcc ccgtcggctt tttcttactt
     1201 gctggttttc tagctcctag tatcaaaacg tattttccat gatcttgatc aaaaattgca
     1261 aaaggatcgc gccaatcatt cttatcatat ccttcttgag gaacaagttt tgctgcttcg
     1321 ccatcctttt tccaatgaat taaatcttta gaacttgcaa tcattaacac ttctgaagtt
     1381 tttccatcca acttagcttt gcgattatgt ccagtataca aagctaaata ctcattgatt
     1441 ccttggacaa tacttccagc gtaaatgtat tgatcaatgt catcgttgcc accacgatga
     1501 attacttccc cgtattcttc aaatttcaca aaatcagtgg ttgtaactag tgaccaccca
     1561 aaaggatcag ttaaaggttt aggatttctc gtatctcgtt gatgaaatag ataaaaagtt
     1621 ccatctttat aaaaaggcat gcaatcccca aaccaaaaat ctttaggctt gtaaaaaatt
     1681 ttttgcattt atcactcatc ctcttcgtta gtatgtcttt gcatatctgc aagacacgca
     1741 attgtattca tatttcttag attttcgatg atatcatcgc ttttttttcc ttgtaatctc
     1801 aaattgacag cctcaatcaa aattcctaaa ttcatgccac caagaagttg aaacccattt
     1861 tgaaatactt cacttctttt agcaaattcg ttgaaaggtg ttccaccaat caagtcagca
     1921 aataccataa cttcttgatt ttttacttga gaaactattt tttcaatgtt ttcacggaat
     1981 tcttcaatgc ctgcatctcc aagtgttacc acatgacaat ttaaattatc gccaaaaaac
     2041 atttttaatg tatctacaaa tccaatggca atatttccat gagatgtaat tataaactcc
     2101 atccactcat cctctgttct taataaataa aatcctttaa aaattctttt gaatctctag
     2161 gagtttgtat cactgtcact tcaactccat cactaattag ttctttaaaa gcctctctct
     2221 cttcttcagt aatatttatc gtcggacgga tgcgttctgt tccattcctt cttgataaat
     2281 ttcctacgtt tacacttgtt attggtaaac cgttatcaat caaaaattta acatcgattg
     2341 gagaaactgc aacaataaga actttttgtc cttcatattt accagaaaga atatttttaa
     2401 ctgctacttc ccttgtaatt attgaagttc taacattagg tggtgcagcg aggcgaacta
     2461 cagctttttt tacgtcatca ttggctactt cattattaat aaccataatt ctgttagcac
     2521 tcaattgatt tgtccattgt gttgctactt gtccatgaat cattcgatca tcaatacgaa
     2581 catgtataat acctttcatt ttttcatcca tattcaacac ctacttcaaa atatttaacg
     2641 ctcttaacgc aatagaaact actaagatta atagaattac ataaactggt ttaacctttc
     2701 gtcctaacaa ataatacaca attgctacta cacatgcagg taataaacca ggcataactt
     2761 gatcaaagat ttcatttccg gtcattgtta agtcgccttg tttaaattca aagccaacat
     2821 tcacttttac aactgtggca atgagtgcac cgaccaccat taaccctaaa attgatactg
     2881 catcagtaat agactttaat tttgtttcaa tttgagttag taggctcgca cccgattctt
     2941 tcccagcaag gtataaagga taagcagtaa tccttaataa aattgatgcc agtatccaca
     3001 aaataattcc aacaaaacta ccttccaatg ccatattagc tgcaattgct ccaaaaatag
     3061 tccatggaat aactacaaat acactatctc caattcccgc taaaggaccc attagacctg
     3121 ttttcagagc acttactgct tccaaagact tatttttttc ttctttttca attccaacat
     3181 tcataccaat aatgtaaggc gctgtatacg gcgtagtgtt aaaaaattgt aggtgagcta
     3241 aaacggattg tttcattcct tcaggatcat ctttatatgt gtccttgata aaaggaagca
     3301 tagaatacgc ataacccaac ccttccattt tttcataatt taatgaagtt gcaccattga
     3361 gaatccaccg gaaaattact tgtttgtcga tttttgtcag tttcttattc atcttcgtca
     3421 aaacctcctg ttgcagctgg tgctacttga gtattatttg atctattttt atatacgatt
     3481 agtgcgattg cagcgccaag taacgaaatc cctaaaattg gtatttggaa ataaactgcc
     3541 aaaacgaaac caactaacaa ataggcaaag taatcttttg taggtaaata tttaagtagc
     3601 attcctatcc caacagcagg taatagtccc cctgctacct ttaaaccacc ggtaacccat
     3661 tcaggaatat aattgattgc acttcccact aacgttgggc ctaatatcac agttaacaaa
     3721 acaggaattc cagatacaaa gcttgtatta attgatgcca aatattgcat ccactctaat
     3781 ttttgataat cttctttttg tgcgaatttt tctgcctttt gttgaaaaaa aatgtttgtt
     3841 gaccacttta gaacatctag ttgtacaatt aacattgcta ccggtattgc taaagttatt
     3901 cctatagaag catcctggtt agttgagatt gctaaataag taccgatcaa tgctgctgtt
     3961 tgataatccg gtactgatgc accaccaaaa ctagtaatac ctagtgtcat taattgcaaa
     4021 gtcccaccga tataaagtcc agtttgaata tctcccataa taagtccggt caagaaacct
     4081 gcaacgacag gttgggaggt tccgattttg ggcccttcct tatcccagtt aatgaaaaat
     4141 ccatacaaca caactaaaaa tgtttgccaa aacgccattt tgttctctcc tttcttctta
     4201 tacttttata taagcaatta ctgtgccaac taacattatc caataaagcg ggaaaataca
     4261 gtaaat
//
LOCUS       DAJSKS010000174         4243 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJSKS010000174 DAJSKS010000000
VERSION     DAJSKS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.181"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            75..503
                     /locus_tag="ON396_002599"
     CDS             75..503
                     /locus_tag="ON396_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3114640.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="ON396_002600"
     CDS             490..732
                     /locus_tag="ON396_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114641.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="ON396_002601"
     CDS             complement(1032..1247)
                     /locus_tag="ON396_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114642.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="ON396_002602"
     CDS             1354..1668
                     /locus_tag="ON396_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114643.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="ON396_002603"
     CDS             1772..2950
                     /locus_tag="ON396_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3114644.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            3353..3646
                     /locus_tag="ON396_002604"
     CDS             3353..3646
                     /locus_tag="ON396_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HCR3114645.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1449 a          638 c          821 g         1335 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3241 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
     3301 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
     3361 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
     3421 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
     3481 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
     3541 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
     3601 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
     3661 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
     3721 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
     3781 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
     3841 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
     3901 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
     3961 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
     4021 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
     4081 cattttttaa gtgaatacaa gatcagctag ttgaacaccg tttattcgtg atgagggaca
     4141 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
     4201 gaaaaatagt agataatccc gtcctaactc ttttattctt ccc
//
LOCUS       DAJSKS010000175         4240 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJSKS010000175 DAJSKS010000000
VERSION     DAJSKS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.182"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            40..477
                     /locus_tag="ON396_002605"
     CDS             40..477
                     /locus_tag="ON396_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114646.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            572..1456
                     /locus_tag="ON396_002606"
     CDS             572..1456
                     /locus_tag="ON396_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCR3114647.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1678..>4240
                     /locus_tag="ON396_002607"
     CDS             1678..>4240
                     /locus_tag="ON396_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114648.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
BASE COUNT         1387 a          712 c          959 g         1182 t
ORIGIN      
        1 aagaaaacga tattaaaaaa tgcacgtaga agtagataaa tggagtggtt ttgcaaattt
       61 cttttatttc tagaagttat tcacgtatac ggatttgttt ttctcacaaa taaaaaggca
      121 ggaagtacaa tggatcattt gtatataaaa tatattcatc aattttccgt tttggtatta
      181 ggcacactaa ttatagcggg gattgttgca tttattattc aaggtcgtga catattaaca
      241 gtacatcaag agtttttcag ttatgcattt ggtattcctc tgatcgctat aaggtctgat
      301 aacaaaaagt ggaagaaacg ctattacatt ctcttgctat ttttgtgttt ttatctgcca
      361 cctaagattt ttactttatt aggtatacaa tgcctatcat tttcttttat ttcctatatc
      421 attgttttaa ttttcctcag tttcatagta tgggtatttt atcttgtcaa aaaataaatg
      481 aatgtttcat gaacagtact tcatcacagc ctaatttttt tctttatgct atcgttatat
      541 taaagaaatc aatcagaagg gaaagaaagt gatgaaaaaa gttttattag tggtaaaccc
      601 cagttcaggc ggtgaacaag caaaggaatt tgagcaatta gcgatagcaa aattggaatc
      661 ggtgtttgat gaagtggtcg tcttgcatac aaaaaaagca ggggatgcaa aaaactttac
      721 tcgcgaagcg gctacggaag ggtatcatag tgtgtttgtc atgggcggag atggaacagt
      781 caacgaagga atcagtggaa tcgctgaaca agaacatcgg ccaaatttcg gatttttccc
      841 attgggtacg gtgaatgact tggctcgagc attagggata ccgctagaac ctgaggaagc
      901 gatcaatcat ttttcgattg aatcagtcaa gccattggat attggaaaaa taaatgacga
      961 ctattttatg aatgtcgtcg caattggctc tatccctgaa gcaatcaatg atgtagatgc
     1021 ggagaagaag acgaaatttg gaaaactagc ttatttcatg tcaggaatca aacagttagc
     1081 ttccacacaa tcctactcgt ttcacgtaga agttgatgga aagaaggaag aaattgagag
     1141 cagtatcttg ctgataggac taactaactc agtaggcggt tttgaaacat tacttccaaa
     1201 tgcaaaagtg gatgatggta agctccattt ggtttattta aaagattctt ctttgatcga
     1261 tacgcttaaa acattgccag acctattaaa aggagtagat gaatctacga aaaatctagc
     1321 ctatcttact tgtgagcaat tagttgtgtc attagctgat gaggaagaat tgacgacgaa
     1381 tgttgatgga gatgaagggg ataaattacc tgtgacaatc aaagtgttac cctcacattt
     1441 acaggtttat tgttaaaatg gaggttgtga ccgaagagtt gtcaggagga catcggttgt
     1501 tcggaataag ggactgtgac aagacttttg tcacagcctt tttttgtaca aaaaagaaat
     1561 tttcacctaa cagaaaaatt tgccataaaa atacatattt taaatatgaa agtacaatta
     1621 caaataaatt aaaacgttaa aataaaattg tcaaaacgtc agagaggatg gtggagaatg
     1681 aatcaatatg taaaaaagct gattgcgtcg attttgtttg tcttccctct cttgatggtg
     1741 acatccgttt ttgccgatgt agagaaaaca agcgaatcaa cgcaatcaga gaatcaaagt
     1801 tttgacaaga ctactttttc agaagaatca attacttctg aaactacaga atcccaaagt
     1861 gaggcgccta cttctaatac aaggcaagaa gaaaataaga caagtgagac ctcagaagta
     1921 gctgaacaaa cgtctgatag cagtgaagag gtacaggtta gagttgtttc agcggatggg
     1981 tacagcgaag ctgccacaac agaagaactg acgcagcttc tcgcagacga atcagtagct
     2041 aagattcgtt taactcagcc tttgacttta gacagggaat tggagatcaa gcgagatatc
     2101 gttatcgatt ttggcggact tgctcataat ttcggcacac atcacatcta tatcaatgag
     2161 acacctcagt cgatcgagtt tcagaatttt aaaggaacgg cagctcatcc aggtgggttg
     2221 ataccaaata tcgatggaaa tgccattatc attgcttata tgtccgattt ttggggaaac
     2281 agatatcatt ttacgggaga aataaaattt accgggacac ttgacttata tgatggttct
     2341 aaattaggcc tgatttacgc ccctcgtgca actgtcacat tagatggtgt ttccggtgta
     2401 ttggatgtcc aaccagtgaa agaagggatg aatgccgctc cgggtaaagc ttatttttgt
     2461 agaagctatc agctaaatgt aatcaatggt agtcagcttt atggaccata tctaggaaaa
     2521 ttctatggtt ttctagattc aggtgatgag acgggcagtg gaaaaacagc accaggactc
     2581 catatcttga gtggttcaaa ggtctctctt gactatgatc gggcagatgg cgtcagcgat
     2641 gaaggagagg ctgtcgatac gctacccggg aatgtcacat ttaaagtcag tggctcagga
     2701 agtgaatttt cggtaaatac aaagatcaat attaccaacg acaacaatcg cggaattatc
     2761 caaatgagag gctcaggatc aagagtcgct gtgtcgaatg atgggaaaat cacaatcaat
     2821 acagcgacta ctggcggatt tcgcctgcaa ggagattcta gtcaaatcca tgtatcaagt
     2881 ggtggacaaa tctcggtcaa aatggctgga gatggcgacc aacctggaaa caatgggatg
     2941 cgttttgtag ggaaaggact ttctttgatc gtagaaggag ccggatcagt catcgatatt
     3001 gataagcaaa gtggtcgttc tagcgcagtt cgttttgaga acggtacgca aaaactaaca
     3061 gtaactaatg gaggctcatt gaaggtaaga aatgcagggg atggcaaatc ttatgtgaac
     3121 agactcaatc aaggaaacca agcgatccaa ttttatgacg cttctggttt tctagaaagt
     3181 ccagggtcag ctgattttac ggttactgga gaaaaatcaa atatcgatat acgcgcagat
     3241 tccggagcta ccgtagatac aactggagat attgatttga actttatggc tggcgaaaaa
     3301 acttatttag tcatggtcgg gaaaactggg gaagataaga ctggcatcat cagtggggat
     3361 gtgttgaatg tagacttaga atctcctacc tatttcgatt tccaaaacaa acggatcgga
     3421 agcgagacta atggcatagg tggctgggta tttcaaggaa atggcgattc ttctttgaag
     3481 atcgctcatt cagaaattgc tttatggcga aaggcaggag taacgttcga taactttgaa
     3541 aaagatccgg attatttttc tgatatgaca gacttatcga tcaatggaac cgacttaggt
     3601 aattttgtat caagccctag tgcaggttta acaaatgaat ttgctcagac aggtgccgga
     3661 atgaaaaatt ataatcgaat cagtgccaat aaccagcttc cgatgattga atccttaaga
     3721 gtaccaacga atgcagataa aaaaatctat gggcatgtcg ttgttcccga aggtgtcgag
     3781 gcaaagccaa gagatgcttg gacaggcgaa gtagaagtga ctttacgtat tacttatgca
     3841 gatacgacga aatcacctgt agaaattact gctctcacac aaggacaaac agatgaaaat
     3901 gaagggacag gatataatcc gtggggagaa ggcgaacaag gcggtttgtt tgaggttcaa
     3961 gtacccaacg aagaaagact aacaacgggg gatcaagtgc aaatagttgc tgctaaaaaa
     4021 gtaagaggcg gccaagaagt gacagaactg gactctgcca aaacgacagt cgatgtgatt
     4081 cctcctgagc ctgctaaatt aagtaccaca ttgatcaatg cagcttctcg ctcaatcaca
     4141 ggtacaggcg aagcaggagc tttggtttca ttgaaaaaaa atcaagctgt tttgtccgaa
     4201 acaactattg atgatacagg taaatttgtt ctttctatcc
//
LOCUS       DAJSKS010000176         4144 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJSKS010000176 DAJSKS010000000
VERSION     DAJSKS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.183"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(20..1786)
                     /locus_tag="ON396_002608"
     CDS             complement(20..1786)
                     /locus_tag="ON396_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3114649.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1746..3500)
                     /locus_tag="ON396_002609"
     CDS             complement(1746..3500)
                     /locus_tag="ON396_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3114650.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3610..>4144)
                     /locus_tag="ON396_002610"
     CDS             complement(3610..>4144)
                     /locus_tag="ON396_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HCR3114651.1"
                     /translation="TVISLVIYFFSIMVSSIGGAFGHSISPGIFGLLSGIIFVYISYN
                     YPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQVASW
                     ALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLVVASL
                     SMIGVLIGRKVLKKYKK"
BASE COUNT         1396 a          790 c          586 g         1372 t
ORIGIN      
        1 taatttaaag cctatttcat catcgtgatt gagcttcttt ttcatagacg aatttctcag
       61 atagtttagt tttctttatg agatttttgt atatttctga ccttcgatac aactcattat
      121 gagtaccttg atcaactagt tttccttgat ccatcactac aattttattt accttttcaa
      181 tagacttcaa gcgatgcgat ataattagta ctgtcttatc ttgtattaaa cgatttaaac
      241 tttcctgaat acgtttttca ttttccgcat ctaaactggc agtaatttca tctaaaatta
      301 aaattggcgc atttttcaaa aaagcccgtg ctatggatag gcgttgtcgc tctcctccag
      361 aaagtgaact tccgttttct ccgaccaatg tatgataacc gtcaggtagt ttttcaataa
      421 agtcttcgca gttagctaaa cgagctgctt ttttcacttc ctcatctgac gcttgactat
      481 ttccaatacg tatattttcc aaaatacttg tattaaacaa agttacctct tgaaaaacaa
      541 tagcgatttt gctaaacaaa gaagctggag atacacgttt aatatcatac ccatcaatta
      601 aaatacagcc ttcatcataa tcatacagtc tagacactaa tttcaagata ctagttttcc
      661 cggaaccact agcacctact aacgcagtca cttctccttg tttagccgta aaagagatat
      721 gatctaaaat tggggtattg ttatcatatg agaaagagac atctcgcaac tcaatgtcaa
      781 aagatttcag aggactatct gatccctctt ggatactggt ttccttcata gctctaattc
      841 gctgtatttt aggagctaaa taaaatattt caagcactgc ttctttcata gaatcgaagg
      901 aatcctttat ttttattgcg gctagtaagt aaccaacgac gtagagtata gtcacttcac
      961 ccgttaacag taagtgaaca cctactagaa tgactactgc taaactaaca tatgagaaaa
     1021 tagaggataa ggccatgact gaaaaagtac ttaattctac ctttaaatga atcctttcac
     1081 tctcttccat ttttttgaaa agtctgtctt gaactttttt agatagatta aagctattaa
     1141 tctcttgctg caattctata gtttcttgaa attcttccga gttttctctc aaagtatcgt
     1201 aatatttagt attggctttc gtttgggatt tctttgataa caagattaag acaaaactaa
     1261 ataacgttgg caaaataaca gctaatccca ttttgacatt accaaccaaa agcatcactg
     1321 aaataaaagg gaaaaacaga gccataccgc cggctttagg tattgcatga ctcatcgcat
     1381 gctcaatacc ttcaacgtca gacatgatag tttgagataa atctgataaa ttatgttttg
     1441 aaaaatagga taatggtaaa tttgacaaat cgtctgcaat ttgcactctt aaatgcgcac
     1501 tttctttata agtactatta tataagtttt catactccct atctaacaac caatacatga
     1561 aaagcaaagt aacaaacgat acggcaaaat aaagccaacg gggctttgta ttttctaaaa
     1621 ctaattcgtc tatcagcatc attataatga atgctggtac catattaatg cagtatacaa
     1681 aaaaactaga aatagaagct ttagttaaag cttctgcgcc ctgatcactt aaagcaaatt
     1741 tgtttttata aaaacttttc attgtcaagc ctccaatcat ttgcattctc ataattagtg
     1801 accaattgtt tatacacttg atgtttagcc attaattcat tatgatttcc acgttctact
     1861 attttccctt ctgacaagac aagaatttca tctacagatt taatgctgga taatcgatga
     1921 gctatcataa taactgtttt ttcttttatc agatgttgaa aagccttttg tagttcatac
     1981 tcattatctg gatcaataga tgcactggct tcatccataa taacaatctt tgcatctttt
     2041 aaaattgctc tggcaatagc tatacgttgt ttttctcctc ctgacagata aactccttta
     2101 ctaccaatta aggtatgctc tctttcggga aatttttcta aaatcgattc gcaaccggct
     2161 aatcttagcg cgtcaaatac tttacttgct ggcgcttgag gatttgctat agaaacgtta
     2221 tcgtaaatac tctgattgaa tagtttcgta tcttgaaaga caaaagcaat tgtgttaata
     2281 attgtgtctt ttgaatagtg atttaaaggc ttaccaccaa ttttaatctg tcccttatcc
     2341 acattgtaga aacctgaaat tagtttagct atcgtactct ttccgcttcc tgattttcct
     2401 actaatgcat aaattctttt ttgttctaaa actaagttga aattttctaa aacacactct
     2461 tctttgtatg aaaagctgac attttcaaat gcaatgtcac aatttttaat ctgtgtctcg
     2521 ttcccatatt ttagttggtc ttgcttcatt tctaaataaa gggagtctag tgtttcgact
     2581 gcatatcttg cttcaaacaa gtgcatactt acatacatta ttctcatgaa agaaacaaaa
     2641 ataattccac ttaaaaaaaa caacatcaac aagtcaacag ctaatatctt gggagtggta
     2701 agagaagtta taaaaaaggt tataggaact atcaaaatgc aaactatgcc aaaaaatagc
     2761 cattgaaaaa gaacaaaagg tgttttccca ctcaaagaat acttatatgc aaacttagca
     2821 taatcgttaa ttgctttatt caaggcttta aatgatgtta ctttagcacc aaatatcttt
     2881 ataacttgaa ttccgcggat atattctacc gtttcagagc ttaacacatc taacgattct
     2941 tgatattttt gaataaaatt tgtattctct gtcattttct ttaacaagaa tacaccaatt
     3001 actaacatgg cgattagaac caatccaatt ttaaagttaa tgaaaaagcc taaggcaact
     3061 gataaaacag gtgttactat ggcttgagca ctatctggaa tcaagtgagc aattgccata
     3121 tgtgttttag ccgcgttgtc atcaattgtc tttcgaatat atcccgaaga atttaaatca
     3181 aaaaaacgaa aacttgcttc tgttaaaccg ctaattccct tttttctcaa attagtctct
     3241 aagcgaaacc caaggatatg agaaaataag ccagaaaaaa tataaaataa agctcctaaa
     3301 gttaaccaag caacagtttg aaccgcataa ctcttagcta aatattcatc tcctacaata
     3361 attagagact gaagaaattt gtaaatactg taatatccat agactgttaa tacgacagaa
     3421 aaatcagaaa ataaagtggc taaccatccg tagtatttca ttttagggac ataactaaat
     3481 aattttttgt atactttcat tttttcctcc cccgcattca atttcttctc ttattcgttt
     3541 acaactgtta atgctttccg taatagcgta aattagagat gtacaaacag ataaaaggta
     3601 aactacgttc tattttttgt atttttttaa gacttttctt ccaatcaata caccaatcat
     3661 agataaactt gctacaacta acactgaaat gattccccat atacctaccg catagtggat
     3721 cattgcatcc attgttgcag cagattgtcc tttatctatc caatcttgac gaaaacgatc
     3781 tgtaaagaac catataggaa tccactgtcc ggcagcactg cctaattgca ttagagccca
     3841 agacgcaact tgaggaattg ctcgatcata tccaaataca gatagaataa tatcagctaa
     3901 gatcgccata ctaatcgaac tgataaacca aggtaagtac gccccgccca ttaacgtaaa
     3961 aaataacaat aaaacaattg tgtaaatagt aaaaattcct tttctagggt agttatagct
     4021 aatataaaca aatatgattc cagataataa tccaaaaatc ccgggggata tgctgtgacc
     4081 aaacgcccca ccaatactac taaccattat tgaaaaaaag taaattacta gggaaataac
     4141 tgta
//
LOCUS       DAJSKS010000177         4097 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJSKS010000177 DAJSKS010000000
VERSION     DAJSKS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.184"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            54..1355
                     /locus_tag="ON396_002611"
     CDS             54..1355
                     /locus_tag="ON396_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3114652.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1382..1642
                     /locus_tag="ON396_002612"
     CDS             1382..1642
                     /locus_tag="ON396_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR3114653.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2641..3876)
                     /locus_tag="ON396_002613"
     CDS             complement(2641..3876)
                     /locus_tag="ON396_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCR3114654.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1165 a          664 c          859 g         1409 t
ORIGIN      
        1 ttgcgttgtc atgcaatgaa tggttcatat tttattgaaa aggagtggtt ttagtggaaa
       61 agctgatgaa atgggtcgaa gagaaacttg tcccaccaat ggcaaaaatt ggtacacagc
      121 gtcatttatt agcaattcgt aatggggttg tttcaacatt gtcattgatt ttgattggga
      181 cattttttat ggtctttatc aatttaccgt ttcctggatg gaatgaattt attgcccctt
      241 attcggcaac gattgtttta ccctttagga tgacgatggg attgatggct atttatgcaa
      301 cttttgttat gggttcggat ctagctaaat catatggatt agatagtgtg acaggtggta
      361 tattatcttt aggaacgttc tttatgttac aagttcctgt aaacgtttta actcctgaag
      421 aggcacctct tggctgggtc ttacctatga gttctttggg agcatctggg atgtttgcgg
      481 ggatcttatc tatgattttt gcagtggaag tatatcgttt ctttaaaaat cgaaatatta
      541 ctatcaaaat gccagaacaa gtgccacctg ctgttgcccg tagttttgag gcattgattc
      601 caggtgcagt cattttaaca attacttggc tgattcgaag cgtcgcgggc tttgatgtaa
      661 acgctgcctt attatcgtta ttcgagcctt taaccaatat tttagggaat aatttgcttg
      721 gtgttttact accaatgttt ttgatccatt tgttgtggtc atttggtatc catggcatga
      781 gtattgtagg ggctgttgtt cgtccaatgt ggctgatcat gttggatgag aatgcaaagg
      841 cattggcaga tggtacagct gccacccaat taccctatat cgctcctgaa cagttttatc
      901 aatggacggt aacaattggt ggtgcaggag caacgatcgt ggtatctgtt ttgtttatat
      961 tcttctgtaa atcaaaattc ttgaaagagg ttggtcgttt ttctattatt cctggtatct
     1021 tcaatatcaa tgagcccata atttttggag ctcctatgat gttgaatcct tacatgttca
     1081 tcccgttcaa cttagttcct ttagttttga ctattatcag ttattttgca gtcaaattag
     1141 aaatggtcaa tagatttact gttttgccaa catggacatt accagctcca atcggcggtt
     1201 atctttcagc aggaaatgac tggcgtgttg ttatcttgat catgatcaac acattgatag
     1261 catttattat ctattatcca tttgtaaaag cttatgataa aaagatgctg tcagatgaac
     1321 aaagtcgtgg agaaaacgaa gtccagactg tctaagtttg gtttttagaa ggtgggaaac
     1381 aatgtattgg ctgattttat tttttgtttt tatcttttta ttaacggcta gtcaccttat
     1441 attgaatatg ctagctacat atcatattca aatcaatcga tggatttggg cactggcatc
     1501 atttttgatc gtgattcttc caaagatcat tgtaccgcat atgaatgttt tgttttcatg
     1561 ggggacatat gtactttgtg gaatttttgc tatcaatttt atgatcgagc agcatagatg
     1621 gtttgtcaca tcgaagcttt aaatgttcta aaagtggttg taacagaagt ggttagcttc
     1681 aagaaataag aagaaattta cgaaaattgc ttcttaaatt tttgtgaaat gttggcttat
     1741 ttttcgtttg gttgaactta ttaccgcaga tgcaagcttt tgaccaccgt ttattcggaa
     1801 tatgggactg tgacaaggct tttgtcacag ccctttttta cataaagatt attattgttt
     1861 taactgtttt ggcagaaaga aggaggaaaa aatggaaatt ttatcagcaa tggatttaga
     1921 attagtagat ctactaccaa agtgatcaag tcaaatatag atttgggagc ttacaaccat
     1981 atagtagggg ctgcagtatg gtgtatgaat ggtaatatgt atgatgtgag tgtttattca
     2041 aatcatgaaa cgtgtataga aataattgtg ctgggaatag caatcagtca tggtgaacgt
     2101 gacttttagt gtatcgttgc tgacgacata gaacattctg acatgatcta ctcaatatgt
     2161 ggggattgct ggcagtttat ataggagtat gcatcggatt gcgagatcat tgtctcaaca
     2221 gaagagggag gttgattatc aaagataaag tggtaaatcc tacatttgat ttagtaattg
     2281 ttgaggggac tagcggcaca aatctctaca aagcgatgct tctagatcta tatgacacat
     2341 tttaagaaaa tgtatttgcc taattattat ggtctttctt ttgaagaaac gttgagatga
     2401 tccaaccaga gaaaggtgtc aaagaatttg actaagaata aatggcagac gctacctatg
     2461 ctcatttgga tagcgtcaga tcttttattt taaggatgat tttgtgtttt ttagacaccg
     2521 taatttaagt aactctaact tttcaatcca ctatttactt tttttgtatt ttcactgtct
     2581 gggtctcgaa tagcgaaatt actgatgagt ctctattaaa aaagcttttt ctacttgaaa
     2641 ctatattggg tggttgctca ggaaggtttc gatttcttga attgcttgtt gtggaccatt
     2701 tattggaaaa acggcttgca gcgtgcgtgc ctgctgacga atcttgggtg aagaaacttc
     2761 gcgcagagca atctgcagtt ttctttcagt cactggtttt gtgagggcga ttgcgtttcc
     2821 gtattttaca caatagtcaa tattacagct ttgctcaaat tgcatcccca caccgataaa
     2881 cggtttgccg gattcacagg cagtttggac ggtcccttcc ccgccgtgga taaaagaaaa
     2941 atcaatgaat tcatgcaaaa gatgactaga cagatattct gttattaaaa gatcttctga
     3001 gtcagattga atgtcatcaa gcagatagtc aataggtgca ataaccgcta gatccggttg
     3061 tttttgtaag aagaacaaaa ttttgcgtaa catacgcgga ttgcctgaac tacccattgc
     3121 gaaatagata atacgttttc tttcttgttg ctttcgctgt ataaaagtta gcacttcttc
     3181 aggcaaggaa gtactgagct tagcaaaaat tggaccaacg attcgataat tttccggcag
     3241 ctgttcttct tccaaaaata gtttaggcgt tgtgatgaga tttagatcag catccatcat
     3301 ttgtaaactt gtttttggta aaggaagcat ttctttttgt gcagcgtgtt tgaaggcatt
     3361 ggggatccat cgggtattta ctatacgctt ttttgtccag tgccaaaggg gattgcttaa
     3421 agggccgaag aaacgtaact cgtggggcat cagcgcatgc tctgattgaa aaaaaggttc
     3481 actcaaagca tagggtttaa catatactaa aggaatcttt ttattgcggg ccgataaaaa
     3541 aattgtcaca ttcgaacctg tcacaatcag tcgaggctca aacttagcaa tcagactctg
     3601 ttcactagtg atacgagagg caaccatttc tttggtaaaa gggtttttaa gtgtacgcaa
     3661 ctggtcaaat tttatgattt gttcttcttg ttttttcgta aggatcggcg ctagtaaatg
     3721 atactcaaag ccactttctg tgatcaaagg agcaaaagtt ttagaaaagc caaagaatac
     3781 acagtaataa cggtctttca tagctttggc aacctcaatc attcgtgttg tctcagctaa
     3841 attgaatact gcaggagcaa aaagaattgt cttcatgaaa tcctccttat tggaatgagt
     3901 ctatcccttc gtttcttact agtatactaa aaaacacagc gttcgagtag cctatgtata
     3961 ttttgaatat taaactgtct ctcgctgttt tcacaacttc taaatttaaa ttttgtaatc
     4021 aagttgaaac tccaattttc aaaaaaacac gcaacaattt tttgatcgat ttatatgttg
     4081 tgccagataa atacgag
//
LOCUS       DAJSKS010000178         4088 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJSKS010000178 DAJSKS010000000
VERSION     DAJSKS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.185"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            558..3197
                     /locus_tag="ON396_002614"
     CDS             558..3197
                     /locus_tag="ON396_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR3114655.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3365..4063)
                     /locus_tag="ON396_002615"
     CDS             complement(3365..4063)
                     /locus_tag="ON396_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCR3114656.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
BASE COUNT         1234 a          784 c          904 g         1166 t
ORIGIN      
        1 aaaaagccga gctctcgata agctcggctt tcttgtacct cggaaggatt caaataaaca
       61 gaaatgattg taactttcca tcatacatgc aaagcacggt gttgaatttc ttcatgttaa
      121 acaaaaattt tgttttcgtt acatcttcaa catttgaaac atctcttgtt cctattcaat
      181 tttcagacgt ttacgttatt caattgttac gtatcgcatt acttcttgtt tcggatactt
      241 cgtgatccaa acatccatac gcattcacta tttataggtt tgtactctac acgcatgtct
      301 gggtgaaacc tgtacttcta acaactttgt ttgcttacac tgttctatcg agaaattctt
      361 ctgtctattt gatggtatcc tccttcctta catctatata atagcatcct ttggtagcgt
      421 ttgcaaatat aagcacttta aattttacgc tttcttaaca aataatcgat aagcccttta
      481 tttgaagtaa ataaaaaaag gatgttatag taaaataagt accttttttt gtacgaagat
      541 tttaaaggag gaataggatg gaagaataca agcagacaat agaagaagta aagaaggtaa
      601 cagaagcaga tccagatact gggttgtctt cacaagaagt ggatgaacgg cgaaaaaagc
      661 agggattgaa taaattcgat gaggcgccga aagaatcgat gatcaaaaaa ttctttcgaa
      721 gcttgtccga ttttacaacg atcatccttc ttgtagcagc ggtgatttcc ttttataccg
      781 catttgctac agaacatggg gatttatttg aaggactgct gatcatcgct atcgttgtga
      841 tcaactcggt attggcgatt gtccaagaag gaaacgcaga aaaagcactg gaatccttgc
      901 aagatatgaa taaacaaacg gcaactgtca tacgagacgg caaagtgacc actgttgagt
      961 cagagcaact ggtagtgggt gatattcttg ttttagagtc cggtgatgct atttctgccg
     1021 atgcccgttt gatcgaagca tcacaattac gtgtagaaga atcggcgttg actggagaaa
     1081 gtgaagcagt tgaaaaagat gcagcattta ttgctgaaga agatgagtca ttaggtgacc
     1141 aacttaatat ggtatttaaa ggatgtacgg ttgctgcagg tcgtgggaaa gcgatcgtca
     1201 ctgccattgg catggctaca gaaatgggta agatcgcgga tcttttgaac gaaaatacta
     1261 tgcaaaaaac acctttacag gtccgattga atcagctagg caaacgaatc agtatgatcg
     1321 ctttagcggc tgcggcttta gtatttgtga ttggtgaact gcaaggagaa ccactccttg
     1381 agatgttcat gacttcgatt tccttggctg ttgcagctgt accggaaacc ttgactgtta
     1441 ttgttacgtt gacacttgca tacggtgtgc agaagatggc gaggaaacat gcgatcatcc
     1501 gacagttgcc agcagttgaa acgctaggaa ccgctaatgt tatctgctca gacaaaacag
     1561 gaacgctgac acaaaatgaa atgcgcgtcc gccgggtatg gagcaaagaa gatgaagtaa
     1621 cgaatatcga agattcaatg acgaacagcg cgatggaaat attgaaaatg gctgcactat
     1681 gtacagatgt gacggtcgag caagaagatg acgaccttgt tatcaaaggt aatccaacag
     1741 aagtagccat cgtaagggct gttgaagaga attatcatac aaaggcagaa ttagaagaaa
     1801 aatatccacg agtaaacgag cttccttttg attctgaacg aaaaatgatg acaacagttc
     1861 accaaatggg cgaaaaatac atctcaatca ccaaaggagc attcgatgtt cttgctccgc
     1921 gctttagttc aggcgatgta gaacaagcgg cgatcgttaa tgatcgcttt ggtaaacggg
     1981 cactgagagt gattgctgtt ggttatgcta cttatgacga agaaccgcaa gatatttctt
     2041 ctgaagcact ggaaaaagac ttacgtttga ttggtctgat cggaatgatc gatccaccac
     2101 gtccagaaag taaaggagca atcaagcgag cgaaaaaggc tggcatcaaa acagtcatga
     2161 tcactggaga tcatgtggtg actgccagtg ccatagcgaa agaattaggt atcttgaatg
     2221 atccgtcaga agcactttca ggatcagaac tgcatcaatt atcagacgaa gaattagatg
     2281 cgcgtgtcaa agctctctct gtctatgctc gggtaacgcc agaagacaag atccggatcg
     2341 tcaaatcttg gcaacgtacg ggtgcagtcg tagcgatgac tggggatggt gtgaatgatg
     2401 caccagcgtt gaaagcaagt aacgttgggt gtgccatggg gatcacagga acagatgtgg
     2461 cgcaaagtgc cgcagatatg attttgacgg atgacaactt tgcgacgatc gtagacgctg
     2521 tcagtcaagg acgttcggtt tatcagaata ttcgtaaagc tatcaacttt ttgctaagct
     2581 gtaatatttc ggagatcttt attgttttga ttgcgatgct gttaggatgg ggagcaccat
     2641 ttacagctgt gcaactactg tttgtcaatg tagttgctga tggacttcca ggatttgctt
     2701 taggaagaga gccagcggaa cacggcatca tggatcagcc acccattccg aaaaatgaag
     2761 ggatctttgc tcgaggttta ttgcaaaaaa ttgccataaa tgcaggggtc ttcaccatcg
     2821 taaccttgtt tggttattat ttaggtagtt atgtcgatac tatcagccca tgggtagacg
     2881 caagtcagca tgtcggtcaa actgttgcat tcttgatctt agcttattca tcgattctgc
     2941 atgtgttcaa tgtacgcagc agccaatcga tcttcaaagt caatttagcg accaataaag
     3001 ctttggtaga aatggcgtta ctggcattag ctattacaac ggctgttgct ttgctgccat
     3061 ttacgcaaga actattcggc ctagtacaca tcagtttgaa tcactggttc ttggtaggga
     3121 ttttatctat cgtaccaatc gcggtgaatg aactgatcaa gttccatcga ttaccggaag
     3181 cagaagaaga ggaatgatta actgtttcta agttgatttt ctttttactg gaactataca
     3241 aaaaggaggt gtgacatgaa tgttgtcaca cctccttttt gctaatgggc tgaatgtttc
     3301 ttttactgcc tctcgataaa cggtgttctc ctgccacccc ctcggtcaca agtcacactc
     3361 tcttttaaat atcttttggc aattgttcat aaaatgctgc ttttgttgca gaaatgtatg
     3421 ggctgagcca tagataacca ataccagcag ttgctaatgc taagatatgc cagccgataa
     3481 atgaaagatc taaccagaaa agttgtcctt tataaccatc catcaatttt cgacttgcag
     3541 tgatcgtatc cagtacttta ggtgtttgtc ctgtttcttg gacgatatcg tagtaaataa
     3601 aatagctttg agaataagaa tagcttttaa tgattccagg aataactaat agcaaacccc
     3661 ataatgttgt aaagatcgag atcagcacag ctaagctgat cactccgccg ataaacaaac
     3721 ctttgaatcc acgaaatgca tctttgaatg gttcgatctg atctttttgt ccacgtaaaa
     3781 tatcaagata agtccaagaa ataccgctca tgaacaaagc gctaatgatg ctggaaataa
     3841 ttccgccacc gcctcctgaa ctaccgcctc ctgaagttgt cgcttctgag acgctcgctg
     3901 atccatcata tatggtggtg gcaaacaaga taataggaat cataataaga agaatagtag
     3961 caataccaat caatgtaggg attagattta gtaaaacagc ttgtccccaa cgtcctttta
     4021 gactctcttt tgcttggcgt tttaattctg cagaagattg catgattttt ccctcctttt
     4081 ctttaata
//
LOCUS       DAJSKS010000179         4087 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJSKS010000179 DAJSKS010000000
VERSION     DAJSKS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.186"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(318..467)
                     /locus_tag="ON396_002616"
     CDS             complement(318..467)
                     /locus_tag="ON396_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114657.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            929..1141
                     /locus_tag="ON396_002617"
     CDS             929..1141
                     /locus_tag="ON396_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114658.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1419..3218)
                     /locus_tag="ON396_002618"
     CDS             complement(1419..3218)
                     /locus_tag="ON396_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR3114659.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3342..3938)
                     /locus_tag="ON396_002619"
     CDS             complement(3342..3938)
                     /locus_tag="ON396_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR3114660.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1467 a          718 c          700 g         1202 t
ORIGIN      
        1 atcgaacacc tttggaagta tttttgagtt acgtaagtat tgatgatctg tctaacttaa
       61 tttgacaatt aagattatat taaatattat acagataata acggaatatt atcatcgaaa
      121 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
      181 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
      241 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
      301 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
      361 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
      421 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
      481 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
      541 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
      601 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
      661 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
      721 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
      781 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
      841 atggataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
      901 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
      961 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
     1021 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
     1081 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctgctgaat tggtttctta
     1141 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
     1201 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
     1261 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
     1321 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
     1381 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
     1441 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
     1501 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatagca
     1561 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
     1621 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
     1681 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
     1741 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
     1801 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
     1861 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
     1921 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
     1981 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
     2041 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
     2101 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
     2161 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
     2221 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
     2281 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
     2341 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
     2401 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
     2461 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
     2521 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
     2581 gtcaatgacc agctttcccg aatggctgct cgaaaaggac gatctcgtag aatcatttcc
     2641 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
     2701 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
     2761 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
     2821 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
     2881 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
     2941 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
     3001 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
     3061 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
     3121 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
     3181 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
     3241 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
     3301 tttaacattg tcaatcaatg actaatttta caaaataaat attatccaag aagaccactt
     3361 agatattgac ctccataaat caaggccata ctatataaga aaatcgacag tggtttagca
     3421 atcaaaatga ttagtgtata tcgtttcact gacatgtttg tcaatccagc catcaaacat
     3481 aacgcatcgt caggagcaac tggaagaaaa atagctagag caaacagctt ctcaaaacgg
     3541 ttttgattat ccagccagcc aatatatttg ttatatgtct tatcgctaat catggaaagt
     3601 atgaatggct ttccataacg acgccctagc agaaaaatga taatcgaacc aatgcagatt
     3661 ccaatatagt tatagataaa tccagcataa gggccaaata tcaggacacc agctgcagta
     3721 ctgatccctc caggaatgat tggaataact acttgcagga tttggatgaa gacaaaaata
     3781 atcggtccta aaatgatgga atcacctact aatcctctta acgcattcaa atctttgaaa
     3841 acacctaaat taataaaata aatcgtcagc gcgattgata ggcctaatcc gataatggat
     3901 atgaaattta tcagttttcg agaggcagca atattcatta ttcatcaact ccttagaggt
     3961 tctttgttaa ctatacttta ctagaaaagc aactaactgc gccatttttt gatatgacag
     4021 aaaacaaaca attttgtaag ttcatcataa gtataaaaga ttgatctcaa aaaaacatcc
     4081 aactaaa
//
LOCUS       DAJSKS010000180         4070 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJSKS010000180 DAJSKS010000000
VERSION     DAJSKS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.187"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            86..1435
                     /locus_tag="ON396_002620"
     CDS             86..1435
                     /locus_tag="ON396_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3114661.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1563..2153)
                     /locus_tag="ON396_002621"
     CDS             complement(1563..2153)
                     /locus_tag="ON396_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCR3114662.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2300..3652
                     /gene="brnQ"
                     /locus_tag="ON396_002622"
     CDS             2300..3652
                     /gene="brnQ"
                     /locus_tag="ON396_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCR3114663.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1176 a          744 c          789 g         1361 t
ORIGIN      
        1 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
       61 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
      121 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
      181 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
      241 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
      301 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
      361 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
      421 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
      481 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
      541 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
      601 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
      661 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
      721 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
      781 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
      841 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
      901 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
      961 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
     1021 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
     1081 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
     1141 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
     1201 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
     1261 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
     1321 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
     1381 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
     1441 tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa aattgcttct
     1501 taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc ccagcctctt
     1561 ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata ggaaaaaatc
     1621 caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa aaagacaccg
     1681 gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt tgacaaaact
     1741 ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa gataccaaaa
     1801 gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa agcttcaggg
     1861 ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag caaaaagata
     1921 ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg atgatggttc
     1981 acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt atttcggatg
     2041 acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac aagaactctc
     2101 aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt cacgatgatt
     2161 cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata accatcgttt
     2221 tatataggga attttctgaa aattgttgca tatttttcga aattttgtta cttttaaaaa
     2281 aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc tttttatagg
     2341 ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg ttcatttggg
     2401 tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta ccggtatcgg
     2461 tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg tatatgaatt
     2521 agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt acttagttat
     2581 cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg gtttagcacc
     2641 atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg tattttttgt
     2701 gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg gaaagttcct
     2761 gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt tgaatccact
     2821 aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt ttttcacagg
     2881 gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg ggatcatcat
     2941 tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta aagacacgat
     3001 caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc tcgcttattt
     3061 aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca cgctggctca
     3121 gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta tcgttatatt
     3181 agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat tcacagattt
     3241 gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc cttgtttagc
     3301 ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga tgttcatcta
     3361 tccattagca atgacactga ttttactagt gttagttggt cctttgttta agcagcgtac
     3421 ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg atgggttgaa
     3481 cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat atgttgcgga
     3541 aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag ttatcggttt
     3601 tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt gataaaagag
     3661 gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa tggcttctca
     3721 catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc gtttattgag
     3781 aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa aatggcttct
     3841 cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca ccgtttattg
     3901 agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc tccatatact
     3961 ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat ttcttttata
     4021 atatagattt tagacatata tatgatataa aaatagtttt cgcaaaaaaa
//
LOCUS       DAJSKS010000181         4024 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJSKS010000181 DAJSKS010000000
VERSION     DAJSKS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.189"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(133..1638)
                     /locus_tag="ON396_002623"
     CDS             complement(133..1638)
                     /locus_tag="ON396_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR3114664.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1635..2321)
                     /locus_tag="ON396_002624"
     CDS             complement(1635..2321)
                     /locus_tag="ON396_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3114665.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2514..3935)
                     /gene="gndA"
                     /locus_tag="ON396_002625"
     CDS             complement(2514..3935)
                     /gene="gndA"
                     /locus_tag="ON396_002625"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR3114666.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1107 a          852 c          690 g         1375 t
ORIGIN      
        1 ttaattaacg gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg
       61 aatttctcac ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt
      121 aaagcacatg ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt
      181 cccttgccct actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt
      241 agcgatagac aaccccagac catttccacc cttcgttcta gctcttgcat tatccactcg
      301 ataaaaacga ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg
      361 aatcgcgatc tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt
      421 agttgaatac tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg
      481 ataaatttgc agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg
      541 atataatatc ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc
      601 attcgcataa tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa
      661 gcttttcata cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg
      721 atcatctttc ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt
      781 ccttaactca tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat
      841 acggtcaagc atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat
      901 ttcaggcatg tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag
      961 taccttgagt ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat
     1021 caaagaaaca acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa
     1081 agaagataat tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata
     1141 tataggctgg atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt
     1201 acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc
     1261 caaatcataa acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat
     1321 cttcattaag tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt
     1381 cgctttcgtc agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa
     1441 acgagaagct acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag
     1501 attgatagat gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa
     1561 agaactcgca aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga
     1621 ttttttcggc tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag
     1681 ctttcctccc cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg
     1741 tttgtttcaa cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat
     1801 accacgttca cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca
     1861 attacttcat ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa
     1921 gtaatagtag tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat
     1981 gcacgaagac gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct
     2041 ccgtggtcaa gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt
     2101 ggtgtatttt tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc
     2161 atcagatcca acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg
     2221 ccgttgtagt ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt
     2281 gccaagttct tttcatcttc aatgattaga atattgctca tttattattt tatcctttct
     2341 attctactgg ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag
     2401 tcattttatc atggatagca attttaaaaa agagctgaag tctactcata acaatcgact
     2461 tcagctcttt cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct
     2521 tcatggtacc atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg
     2581 ccaaagtaat cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa
     2641 tcgaagtaag cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag
     2701 gcaacgacgt ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct
     2761 agcaataaat tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat
     2821 cgagctcgga tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc
     2881 caatcaaatt cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt
     2941 ttactgaaat acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac
     3001 tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca
     3061 gaaatatagc gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc
     3121 gcgctttggc ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct
     3181 actactggtt tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct
     3241 atcaaatagc tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct
     3301 actgaaaggt ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct
     3361 ccatactcaa taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata
     3421 tatgtgacac atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca
     3481 actagcgcat aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct
     3541 tcttcaccac cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca
     3601 ttacgacgca tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta
     3661 tcaaggtgag gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt
     3721 agcaagatcc gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct
     3781 ttgaaatttt tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg
     3841 aataaagcca ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg
     3901 gccataccga caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt
     3961 tctccctaat ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat
     4021 attt
//
LOCUS       DAJSKS010000182         3905 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJSKS010000182 DAJSKS010000000
VERSION     DAJSKS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.190"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            43..945
                     /gene="gnd"
                     /locus_tag="ON396_002626"
     CDS             43..945
                     /gene="gnd"
                     /locus_tag="ON396_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HCR3114667.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            947..1948
                     /locus_tag="ON396_002627"
     CDS             947..1948
                     /locus_tag="ON396_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HCR3114668.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1945..2229
                     /locus_tag="ON396_002628"
     CDS             1945..2229
                     /locus_tag="ON396_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR3114669.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2262..3059
                     /locus_tag="ON396_002629"
     CDS             2262..3059
                     /locus_tag="ON396_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR3114670.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3073..>3905
                     /locus_tag="ON396_002630"
     CDS             3073..>3905
                     /locus_tag="ON396_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR3114671.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
BASE COUNT         1246 a          638 c          894 g         1127 t
ORIGIN      
        1 atttttttgt ataatgaggg tataagaaat ggaggcgcta tcatggaaat tggtgtaatt
       61 ggtttgggaa aaatgggatt gaatttagct ttaaacttaa aagagcacca tcacaaagta
      121 caaggactcg acatttcaag tacagctgtt agtcaggcaa gagaaaatga cattcctgct
      181 tatcaagaga tctctgaatt tctagctact tttgagcaga aaaaaatcat ctggttaatg
      241 cttcctgctg gagagataac cgaaaacatg ttaagaaaac tttttccaaa actcggacca
      301 ggagacatta ttattgaagg cggaaactct tattataaag attcaatccg tcgagcagaa
      361 gagtttcaga aaaatgaaat cggttacttg gattgtggga cttctggagg aatcgaagga
      421 gcacgacatc aagcctgctt aatgattgga ggagataaag aaacttttct atccgtggaa
      481 caattattca aagatgtagc aattgaaaga ggatatctgt atgctggtgc atccggaagc
      541 ggacacttct tgaaaatgat ccataatgga atcgaatatg gaatgatgca agcaatcggt
      601 gaaggattcc aacttgtaca aaaaagtgag tatgcttttg acttggaaca agtcgcacgt
      661 gtttggaatc atggttcggt gattcgcggc tggctgatgg aaatcatgga agatcagtta
      721 aacgaacatc ccaatttaag taattatcgg ggaatcgttt ctgcatcagg tgaagcaaaa
      781 tggacggttg aaacagcgct agaaatggct gttccagttc cagtaattgc aactagtttg
      841 ttcatgagga atctttcaca ggaagaagat agtttttcag caaaagtagt ttcagcacta
      901 agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg agtaaaatgc cagattatca
      961 tatagcaatc gttaattcca gcagtttcgg aagagtcttt ccacaccact tggaaagatt
     1021 aaagtcagtt ggagacgttc atttctttac tgttgatcaa gagattccag gaaaagaact
     1081 agcggagtta cttaaaggat tcaatatcat tattgcaagt gttacgccat tttttcgacg
     1141 agattttttt gaaaacaaag atgaactgct attgatttcc cgacatggta ttggttacaa
     1201 caatattgat ttaaaagcag caaaagaaca ccagacaatc gtatctatca tacctgcttt
     1261 agttgaacga aatgctgtag cagaaaataa tataactaat ttgttgtcca tgcttcgacg
     1321 cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg gaagatcggg cggagttcgt
     1381 cggtaggacg ctgtttaaca aaacagttgg tgtgattggt gcaggaaata ccggaagcgg
     1441 agtaatcgaa attttgcgta acggttttcg ctgtgatgtt ctagcttatg atccttacaa
     1501 atcagattta tacatcgaaa gctttggtgc aaaaaaagta gaacttcaag aattactgga
     1561 acaatcagat atcatttgtt tatgtgcgaa tttgacagaa gagaactatc acatgatttc
     1621 tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta tctaatagtg caagaggcgc
     1681 tctagttgat gaagaagcga tgattaatgg tctgaaaagc ggaaagatcg caggatatgc
     1741 cacagatgtt ttagaagaag aaccgggacg tcaagatcat ccgtatttag cgtttgaaca
     1801 agtagtgatt acgccacata tatcggccta cacaagggaa tgtttagaag caatgggtga
     1861 aaaatgtgtc agagatgtcg aacagatcag tcagcacctt cttcctgaac gttctgtcca
     1921 gacaaagagc aactatataa aaggatgatg aagaaaatgg tgaacggttt aaaagtgaaa
     1981 acaggccctc aattttattt atatgaagaa ggtggtattt caaaagtttc tgatctcttg
     2041 aaatcatacg gcgcgaaaag agtactggtc acccacggaa cggtatcatg ggaaaaggcg
     2101 ctacctaaat tagtattttt gaatgacgaa acgattcaat ttttttatca tcgttattct
     2161 ggagaatgta gttatgctga agccagacga atagctacaa tcatcaaaaa aatgaaatcg
     2221 atttcgtagt tggcgttggc ggaggaaaac tgaccgatct agtgggttat agtgccaatc
     2281 ttgcaagaat cgatttcgga gtcatcccta ctttagctag taattgtgct ccatggacac
     2341 ctcttgctgt catgtataag gagaatggac aagcagaagg gaaaacagaa cacttttaca
     2401 gacaagctgc tttttttata acagatcccc aattagtgat tgattcccct gttccatatt
     2461 tcattgccgg gttagcagat acactagcaa aatggtatga atctgagctc atcttagaac
     2521 aagatttttt gcaagatgaa tcatttttga agctagcaca agatactgcg aagatctgca
     2581 aagaagagat tttgaatcat tctgctaaag caatccaaga tatggcacaa agaaaactga
     2641 catcggagtt caaacagctt tcagagatta tctttgctgt tgctggttta gttggaggat
     2701 taggagataa atacgcaaga aatgtggcag cacatgctat gcatgacgct atcagtaaat
     2761 atattccgga aagccatcgt tttctacatg gagaaaaagt agcgtatggg attttctatc
     2821 aactggcact agaaggtcgc tgggaaaaaa tagacgagtt atatcctttg tatcgggaat
     2881 tagaactgcc ggtttctcta gaacaaatgg gattatttcc gaaagataaa cagataatag
     2941 ataaaatggc tgaatttatt gattcgaaag agaaagtgca tttgattcct atagaggtaa
     3001 cacagaaatc actgatacag tcaatgatgg aactggaagg ttatatcaga aagatctgaa
     3061 ggaggaggac acatggaaac attaagtgta gtacaaagtt tgttgattgc tttatgggta
     3121 gccgcggtta tgtcccgctg gttaggtgga ggagccacgt tgaccttacg tttttctcct
     3181 ttgatgactg gattagtcgt cgggttgatc atgggggaag ttgcacaagc gatgattgtt
     3241 acagccgctt tgcaaatgat ttatatgggg gttttttctc caggaggatc aatgccagct
     3301 gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat tattagggga tctgtcgcca
     3361 gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg gaagttactt gtatcagttt
     3421 cgttttttca tcaatacttt tttaggtaaa tatacagaca aagctgtaga agatttgaat
     3481 acaaaagcta tggcccgatc aattattctt tatccaacgg ttgcctcatt tttattattc
     3541 gttccgcttg ttttcattgc tttatattta ggggcgccat tgatcgctga tgtaatcaaa
     3601 gctttagagg gaacagtcgt tatccacatt cttgaagtag taggtggagg gttagcggca
     3661 atcggtatcg cgacaactgt atacgttatc ggtcgaaaag attatctggt ctttttcttc
     3721 cttgcttatt ttatgagtgt tgttttgaaa tctttagata ttacgatggt tacttatgcc
     3781 atttttggga ttattatcgc tttgatcttt gtccagtcgc aaaaagggaa acaagtacag
     3841 cctgccacaa gcaataacaa ttcagcatct tttgatgacg aagatgacta tgatgacgga
     3901 tttta
//
LOCUS       DAJSKS010000183         3759 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJSKS010000183 DAJSKS010000000
VERSION     DAJSKS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.191"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(456..881)
                     /locus_tag="ON396_002631"
     CDS             complement(456..881)
                     /locus_tag="ON396_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114672.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1255..2226
                     /locus_tag="ON396_002632"
     CDS             1255..2226
                     /locus_tag="ON396_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3114673.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2258..3655
                     /locus_tag="ON396_002633"
     CDS             2258..3655
                     /locus_tag="ON396_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3114674.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
BASE COUNT         1289 a          607 c          772 g         1091 t
ORIGIN      
        1 tactacgtga tgtagaataa gcctttctat cgtttggtta ttttatattc aaatatttga
       61 aataaataaa tctttactct ttgatttgta aactgaaatg atccactgtt tgttttactg
      121 atgaagcttg atcaaaggaa gaagctgagt ttgaagttca taaaaatcag ccatagtgag
      181 ttttgcttgg atttgtttat cttcttctac tttttacata attttgaaca ggataagatt
      241 caaatgtttt aaatgaatct ttgactagtt tgtgacagtt gtcgcatatg ctcaagcttc
      301 cttggtcatt aaattctgta tggatatttt tgttgttatt acatgtacaa taatcaggac
      361 tatcgatcat ccatttcgtc tcctttacaa tcatcttgct tctttactat agcagaaaag
      421 aaggggagaa acaaaagaag atccaacaga cggcactata gactgtagat gttgccatcc
      481 tcatccttca aacggtaatt accgattttt gtaaatatga ctgttgtttc ttttttattt
      541 tcttttcctg aaataatata ttcgtcatct ttggtagtga tcttgatatc tgtcagcact
      601 gttttttctt cagatgattc agaagaatca gttccttgtg tctgatacgt gcgtgtccat
      661 tccatttttt tatctgtaaa tgtcaactgt cccgcggata aatccatatc tttacgtact
      721 tttagcgtat atacactgtt ggtggtatct gctttttccg cttttaagac agaagaatcg
      781 gttgtttcgt ccgaggattg gtcagagccg ccaaacaaat cacagccagt caatgtcaaa
      841 agtcccgcag acaaaataaa tagtacgatt aatttcttca aaatacttcc cccatttgat
      901 aggataaaga aattatacca ttggaatgta aaacttcaaa cggatggaag agaaaaacgc
      961 acaaaacacc cacaaataga gtaaatttta gaataaaatt ttaaaaaggc aaaaaatccg
     1021 ccactttcag aaagcgtttt aaaaaagtgt tgcaatcttt taaaaaaatg ctatattaaa
     1081 aatgaaccgg ttcaaatact tgtttaagta taattaatag tgagaggaag ttgctctact
     1141 gaagacttta gttatatgga tattgatgac aagcaacact atgcaaagaa tgcggtcatc
     1201 ataatcgaac aacaagaaag agaaatcaaa tgtaactggc aggagtgaga atgtatggca
     1261 tcgatccgtg atgtggctaa gatggctggt gtttcagtgg gaacagtttc ccgttacttg
     1321 aatggtcagc aactgaagga aaagaacatg aaaaaaatag cagaagcgat ttcagcgttg
     1381 gactataaag aaaacatcat tgctaaaggg ttgaagaata atcgaagctt ctctattgga
     1441 cttttgataa acgggatttc cagccgtttt gggtcggaag ttgtctcagg gatcgaaaga
     1501 gtagctgaag aaaatgggta tagcttatta ttaagcgggt ttgccgatca gccagaaaaa
     1561 attgaacaaa aaattgaata cctgatgaag catgtgatcg atgggctgat tgtcttctta
     1621 tccgaagaag agtggtctgg atttgaaatg ctcacaaaaa tgagtattcc agttatcgca
     1681 ttgaattgtc cagaaggacc agcaggtatt gatacaatat tagtgaatga tcgagaaagt
     1741 gtagcaaaag tgatcgctca tcatggtgaa caaggacata aaaaaatcgg atttattgct
     1801 gccactcaaa cagactacgt agcaagagaa cgtttagcag gagccaaaga agcggtccaa
     1861 aatgatccat cgatccaatt agaggtcttt acaggagact attctagaaa aagcggctat
     1921 tacggggcaa aagcattatt agaaaaaggg gcaactgcta tttttgtcag caattacaat
     1981 atgtcgattg gtgccatcga atgtttcaat caagaaggtg tcagaatcgg gaaggatatt
     2041 gcgttcagtc actatgatta tttagataaa aacgatctga gtatccttcc gaaaatcacg
     2101 atcacgccac caacagataa aatcggggaa cgagcagcag aacagctgtt aaaacgaatc
     2161 aaaaaagaag aggaagaaaa tgaaaaagtg attgtgatgg aaaatatcat acatggaatc
     2221 aactaaaacg aactaaaaaa gaaaaagagg tgctttgatg aaaaaatatc aatttccagc
     2281 aaatttttgg tggggttctg cagcaagtgg cccacaaaca gaaggaagga tttcaggcga
     2341 tggaaaagga gagaacattt ttgattattg gtacaaaaaa gaaccgcaaa agttctttga
     2401 tcaagtagga cctgaaaaaa cctcgcaagt ttatacgaag tatcaagagg atgtacagtt
     2461 gatgaaacag actgggcata attcatttcg aacatctatc caatggagcc gtttgatacc
     2521 tgatgaatct ggaacaatca acgaagaagc cgttcgtttc tatcatcgtt attttgatga
     2581 gttgattgaa aacggtatcg aaccctttgt gaatctgtat catttcgata tgccgatgta
     2641 tctgcaagaa aaaggcggat ggttgaaccg agaaacggtt gaagcctatg aaaaatatgc
     2701 caaaagttgt tttgaattgt ttggttcgaa agtaaaaaaa tggtttactc ataatgaacc
     2761 aatcgttcca gtcgaagcag gttatttata tcgatggcat tatccagaag agtcagatat
     2821 gaaaaaagcg atccaagttg gctatcatga agcgttggct tcagcgttag ccatcaaatc
     2881 ttaccatgaa atggagcaag gtggtgttat tggtatcatc ttgaatctga caccaagcta
     2941 tccaagagac gaatcaaacc ctgaagatgt taaagcagcg caaatagcag atgcgttctt
     3001 caatcgttca ttccttgatc cagctgtaaa aggcgagttt ccaagtgaat tgattgagat
     3061 agtaaaagaa cttgatctca tgcctgtgat ccatgcagag gatttgacgg ttatcaaaga
     3121 aaatacagta gatcttttag gtatcaatta ttaccagcca cgccgggtaa aagcaaaaga
     3181 aacaccaatc gatactaaaa acggtccaat gcctgaggat tatttcgaca attatgaaat
     3241 gcctggtcgt aaaatgaatc cttatcgcgg atgggagatc tatgaacaag gaatctatga
     3301 tattttgatc aatgtacgag atcattatgg aaatatcgac tgctttattt ctgaaaacgg
     3361 tatgggtgta gaaaacgaag cacgatttat caaagaagat ggcatgatcc acgatgatta
     3421 tcggatcgaa tttgtccaat ctcatctgaa atatgtccat caagccattc aagaaggtgc
     3481 caattgttta ggctatcata tgtggacatg tatggataac tggtcgtgga ccaatgcata
     3541 taagaatcga tatggcttta tttcagtaga cttagcacaa gatggaaaaa gaacaatcaa
     3601 aaaatcgggt cattggttca aagacgtatc agataaaaac ggatttgagg cttaagaaag
     3661 aatagttgag agcaaatgcg tgaaattctc cattcttacc attttgtgaa aaagaagttc
     3721 aattatttac gatctttttc aacttttttg tctttctat
//
LOCUS       DAJSKS010000184         3757 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJSKS010000184 DAJSKS010000000
VERSION     DAJSKS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.192"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(591..1148)
                     /locus_tag="ON396_002634"
     CDS             complement(591..1148)
                     /locus_tag="ON396_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114675.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1327..1938)
                     /locus_tag="ON396_002635"
     CDS             complement(1327..1938)
                     /locus_tag="ON396_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114676.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2051..2308)
                     /locus_tag="ON396_002636"
     CDS             complement(2051..2308)
                     /locus_tag="ON396_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114677.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2905..3183)
                     /locus_tag="ON396_002637"
     CDS             complement(2905..3183)
                     /locus_tag="ON396_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114678.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3217..3468)
                     /locus_tag="ON396_002638"
     CDS             complement(3217..3468)
                     /locus_tag="ON396_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114679.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1127 a          775 c          509 g         1346 t
ORIGIN      
        1 gcttctgtcc cagcctcctt attccgaata aatggtgtcc aaaagcttgc atctgccaga
       61 ttatctcatt tcttatgaca agctaagcaa attatctcca ccaaataatt tattcatatt
      121 tttgacttgc gcatactaac taattcttaa attttcatac aaaatttctc aatttccctt
      181 aacgagtaat atttatgtac ggtgattctc actagtatcc caatcacttt ttaagttatc
      241 ccgatttcaa ttctacttca aaaatagttg cttttatgta tgctgttagt tgattagatg
      301 gtcatttgtt gagctaggtc gtttatttta aacaagttgt ttctttagct tatacctatc
      361 tcgtctacca aacgcaagat ggcaaacaca aatgcatacg catctgtatt tgccactttg
      421 attcgtataa agctaacaac tattaactcc tctcagctaa tagttgctct cttttttatt
      481 cagattcatt tttgctgatc acagcctagt atttcaatgg ttttgaacac taaaccaaat
      541 tgttccatta caacattcac tggctgatga aatggattag tagatttcga ttattcaaag
      601 cgattttcta ttggtggatc gtgttcccaa ctaataagac ggattccaaa cctatcgtac
      661 agatcttcta aaccacttat aaattttgca cctatttcag gtgttttatt gcgtggatat
      721 tctattgtta cgccattaaa tggataccaa ttaggattag gctctctttt tttctgtaca
      781 tctcccatag aatagttagc actttctggg acataatata ttcttcgacc atcctctaat
      841 tcatctaata agatatttcg tgatcgtcta tcacttgcta aatgtagacg ccacgtgcga
      901 taccatgcca tgctacctgc atctgcagaa aatccatcaa ataatgcaac aattgtatct
      961 tctatcacaa tcctttcccc atctacttca tattctaatc taaaatcaaa ctcccctcgg
     1021 gtaatttctg gtcttggtgg cttaggaccc aatattattc tacttaaaac cgcattcgtt
     1081 cccagatgaa acacagctat aacaacaata atgattaata ttttgtttct atttgataaa
     1141 tttctcatta ttcgcacatc acctctacat tctcacatat tagttcgtaa tactccttta
     1201 atatcaaaat atctactggt cgtaataata ttcgctatga attcctacta ctcgtataag
     1261 aaaggccaaa tcatcagctt ttacttgctg atgatttggc ttgtttctat ttatcgtaat
     1321 tgttttctaa tcaccaaaat tgttaacaat cggttcagat atttcccagt tgattaaggt
     1381 aattccgtat tgtgtcaaaa cttcttgcgc ttctccgaaa ttaagtacag ttcttatgta
     1441 tccactttct cctaatataa agtctacctc tcttttaaaa ccttcctcga gatttctaag
     1501 ggctatatgt ggttttaatg acattgattg tacattttcc cccatataat attgcgcact
     1561 tcctcgacta aaatagattc ctagatcgtc cccaaaaggt agatcttctc taccactagc
     1621 taatctactt ctccatcttc gttctctttt catagaacct gcgctgaact cacacccgtt
     1681 aaactcagct atgacagtat cttcaattat gttaatctgt ccatctagtt cgtattcaat
     1741 cctaaatgga aattctccat aggtgatttc cggttcgggt attttaggtg agattaatgc
     1801 aaagaaagga aatattaagc ttggcaacat caaaaaaact cctcctatga tcacaattcc
     1861 cgatgtaatc caaaatataa tttgtacaat tctcattgat ccattgtatt ccattttatt
     1921 ccccctatta ctattcatat ccgcctatct atatcgatct tccaatgcag agataattgc
     1981 ttccatacct tgatttttta ctgagttacc tatgcgagat atcattctcc actaaggtgc
     2041 atcatattgt ctatctaaca tgattaattc tccaataggt gtatactcct ccaaaaacaa
     2101 catttaatgc atctccctcg acaacataag ctaccatgtt tgctgtatat gtgtttgagt
     2161 ctaattcata agcagataaa tttactgtcg ctggattaaa tataatcgca ttcgtattta
     2221 tagctactga atttgctgct tcagcaccac cttttgaatg tccgatcatt gtgatttcaa
     2281 tagtaggatt tacgtggaca gccctcaccg tttaccctga ccattataga aatcgcttac
     2341 ttttttgaac tacgcaacaa aggatacaat gctcctaact tcactgtctc catttttgtc
     2401 cactaaggaa tataaaaaat aggcattaat tcatttaatt cggtcacaac acgttacctt
     2461 ttaaatcatt atactgtatc tcatacatta ttatcaataa actattataa aaatgcaaaa
     2521 aatgctttat caatctattt tgtaattggt cataatatca ttgaaatcat accctgtatc
     2581 gatttttcct atgaatctcg tctatttgtg attgacgaaa aagaggcaag acagacactg
     2641 gaatggtaat tttacccact attttgctcc aactatttgt aatcgtaccc acttttgaaa
     2701 taccgagatg atcattttaa ttgatatgct gattcccaag caaaacccca agaaattaaa
     2761 tgctctatcg catctagctt cttgggtttt gcttatataa attccaacat ttaaattttt
     2821 tatgctgttc ggtctcagaa atgccgtttt tgcttatatc acaatatcaa gatgtcaaat
     2881 tattcgtgtt gccgatgact ttgattaatt tttttcaaat tgtttattaa gactaatgct
     2941 gtctttttgc agataccaat gttcgaactt tttaacatta tcatatgatc ctgcaagttt
     3001 taacttctta ccattagatg ttatcaaagt tataattaac cttgcctttt tcccattagg
     3061 atcttttatt ttatgaattt tctgcaggta atctctccta atggcagcta tttttacctc
     3121 atcagtgctg ataaaccatt gatcatccac atacatactg cctgttatgt tgtgtttttc
     3181 catttcttcc gagaacgaaa agtttaaggc tcgttcttac ttccaaagtt gaatgagtcc
     3241 atggattgga cttagtgtga gaattgagaa caacaataag aatccatatc ctcccgcaaa
     3301 catccctaag gctatactgt ctctccatcc ataagtagga ttagaagaga aaagagtcac
     3361 cgtttgtaat aatatgaaaa ggaataatcc agcacaaaca ccaaagataa caactccaaa
     3421 cattgaattt ttatacaatg accatttatt tataggcttt tctttcattt tacttccccc
     3481 tactgttctg ttcacacgga atgaattcaa agttcataag ccctcctctt tgaacatatg
     3541 aatacgactt gctaactata ctttaaaaaa gcttgtttta agcggttata ggcgagttgt
     3601 aatctctatt catctcatta gcaaataact tgccgaaaag tctactgaaa ttaaagcaga
     3661 tagtggaaga attatttagc caggatagac actatttgtg aaacacatgt ctttactttt
     3721 gaaattctat gatgaaaagc cttggaatac agtagct
//
LOCUS       DAJSKS010000185         3719 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJSKS010000185 DAJSKS010000000
VERSION     DAJSKS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.193"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..1353)
                     /locus_tag="ON396_002639"
     CDS             complement(<1..1353)
                     /locus_tag="ON396_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR3114680.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     D"
     gene            complement(1364..2032)
                     /locus_tag="ON396_002640"
     CDS             complement(1364..2032)
                     /locus_tag="ON396_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR3114681.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2162..2746)
                     /locus_tag="ON396_002641"
     CDS             complement(2162..2746)
                     /locus_tag="ON396_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR3114682.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(3077..3310)
                     /locus_tag="ON396_002642"
     CDS             complement(3077..3310)
                     /locus_tag="ON396_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HCR3114683.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3325..>3719)
                     /locus_tag="ON396_002643"
     CDS             complement(3325..>3719)
                     /locus_tag="ON396_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCR3114684.1"
                     /translation="LDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFSKAYNVPSAYIW
                     EINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTNIPIYSTIFTDS
                     IAEKGQDGDSYYAMMKWNLDKIAEGLSK"
BASE COUNT         1245 a          646 c          471 g         1357 t
ORIGIN      
        1 atcttttttt attccaccat tttttaaata aataattcta ttgcatgttt tataaagaaa
       61 ttccggatca tgagtagcta taactactgc acccttttct tttaagtctt taataaggtc
      121 agcaatcctt tccatattaa tataatccaa cccacttgta ggttcatcaa atagaaataa
      181 attagttttt gacaatgaag ctatagaaac caataatctt tgtttttctc caccggataa
      241 atcaaacggt aattgatctt tcattttata aagttccatt ttttttaaaa ttttttcttg
      301 ctgatcttta ctaactattt cattccccag ggctaactcg ctcctcactg attctgtgaa
      361 aaattggtaa tccatttctt gcattactaa aaacggcgat gacaaatttt ttttattttc
      421 gattatcttt cctttggtag ggcgaataat cttcatgagt attcttagta aggttgtttt
      481 ccccacaccg tttttcccta ccaaaccaat aacatctcca actttgacat caaaatttat
      541 gtcttttaga atattttcat aagtaatttc ttcaactttt aatacatctg cacttacctt
      601 ttcgatatag ggaacttcaa tcgaactatt aaaaatgtca aatttcctta atgcgttatt
      661 gttttcagtt tttaacaact gaatagttgt agtttttaca gttctatttt taacaaatag
      721 aacttgatca attaattctt tcaaatagta aaaacgatgt tcagctataa caatagtaaa
      781 tcctttattt tttaaatcac aaagtaagtc agcaagtttc ttagtcgctt tgaagtccaa
      841 atttgaagac ggttcatcca atataattaa tttagagttt agcgataaac tacatccaat
      901 agaaattttc tgccgttcac cacttgatag cgaaaaaatg tttttatcta atatatttga
      961 aagatctaat aattcagcta attcattgat tcttttatcc atatgctctt ttgtaaatcc
     1021 aaagttttcc atcgaaaata ctaactcact cgttgtattt gttgtaaaga attgtcctct
     1081 aggatcttga cttacgtagc caatattttt tgatatactt tcttgtgtta aggattgtag
     1141 cgagttattt tcaaataaaa tttccccaac caattttccg tcataaagtc ttggaataag
     1201 tccattcatt aactttaata gtgtggattt cccactccca gaagaaccag taatcaatgt
     1261 aagactgcct ttttttattg aaaaattaag gtcaacaagc gcttgttcct taacatcctt
     1321 atatgaaaaa gaaacattgt taaattcaaa caattctttc acctcaaatc aaaatactta
     1381 agaaaaaaat agtaacggaa aacaggatga tatagtctaa taagctcatg tttgttttat
     1441 agtaagaact tcttttcaca ggagattcta tcccctttgt tattgcatta gcggtcattt
     1501 cttcagcaat catagttgca cgtataagaa cgggaactaa cagaaattcc aaatattgca
     1561 gaggctgaaa aaaagacaag cgaatgtttc tcatttttaa agattcttta atcattctta
     1621 tttcagaacg ataagtcgga aagaatctga tcactactgt aatacttaac attatttttt
     1681 tcggtaaatg tattttttct aaagaggata ataattcatt gctagtatag acactaacca
     1741 acaaccatga gatcattacg accggcaaca ttcgaaatac aatatataag gatgtcgcaa
     1801 aaaatatagt aaaaaactct atcttgatag catcaataat tttcattact aaaaacagaa
     1861 ctgaataaat caagatgaaa taaatacatg tctttatttt acgaaacatc aatactaaaa
     1921 ctgtagcaat aatgaaacac cccacttgaa atatatttcc ctttcctaaa aaaataatga
     1981 ccggaaaaaa aatatttaaa gtaatcaagg ttaacggatt gatttttgac actctttcac
     2041 ctccaaaaaa taagatcgca ctgttatctt aagctgataa aaatgcaccg ttattcagat
     2101 aacagtgcga tcttatcaaa aagcataact gagaacaatt ctcaatgcaa gcttttttta
     2161 attaactcag taattcaatc cacccaggaa cagaaaattg atacaatctt ctggttaata
     2221 gcatagtctt ttcttttgaa tagctatgct tcaatatatc atagaatgta gcgtagaatg
     2281 agtaaatgta gaagtggatt tcttcttcat taaagactaa attttgattg atcattccga
     2341 tagtctttaa cttatctaga caacagactg tctttttcgt cacaaatgat gtaatttttt
     2401 caacaatatc ttgatatttg gttccagctg attggaataa aatcaaatcg aatatacgtt
     2461 tattctcaaa aatataatta ataaatggat ctaattcatt gaagttctct ctccaacttt
     2521 tctctatatc tatattttct aagtttaaga ggtatttggt aaactcttca ttatacatat
     2581 taataagcgc attaatatgc ggctgaataa tttcttcaaa taacgcttcc ttagaagtaa
     2641 aatgtttata aaaagcaccc gtagttacgc ctgcggtcag gcacagttgc cttatcttag
     2701 tttgctgaaa tccttctttt tcaaaatggc ttagtccact ttctattaac ttttggtgag
     2761 ttataatata atctctggtt tctatgggta cttcctccta ataagatgaa ttaactttat
     2821 tatcttacac aggacttgaa gtttcaatcg ttaacgttta ttactgaact cattccttag
     2881 agctaaacta atgagaaaga gcagaataac tgatacaaaa agcaatccta caagaattaa
     2941 tagactcatc taaatcattc accttttcta aaataatccg agaaagattt aaaaaaatta
     3001 tatccagcta ttaggataag tcctatcata atcactccaa tagccatttt cttatcctcc
     3061 ctaaaaataa agtcttttac ttaaaacctt ttttgtaatc gtcaataaat tttttccctt
     3121 tatcagttaa ttgaaatcct atatctaaat agtcctcata tctaagttta gccagcatct
     3181 cacttacaga tgctggacta acacttagaa cttttgctaa atcattattt tttaatttct
     3241 tttttctttt tgataattcc tgaattgcta gtaaataaat tagtatagta gtcgttttaa
     3301 tttgtaccat acctaccctc caaattattt cgaaaggcct tcagcaattt tatccaggtt
     3361 ccatttcatc atcgcatagt aactatcacc atcttgtcct ttttctgcaa ttgaatcagt
     3421 aaaaatcgtt gaatagatag gaatattggt atcttttgat actgttttca ttggtctatc
     3481 atccacactg ctttctacga ataaggaggg aacttttgtt gtgcgtaatt tttcaactaa
     3541 gtgttttatt tgatctggtg ttccttcttc ttcagtgttg atttcccaaa tgtaagcaga
     3601 aggcacatta tacgctttcg agaaatattt aaagcatcct tcacttgtaa caatcatttt
     3661 tttatcattc ggaattgaag caaatttaga tttagcttct ttgtctagag aatccaatt
//
LOCUS       DAJSKS010000186         3700 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJSKS010000186 DAJSKS010000000
VERSION     DAJSKS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.194"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            52..1851
                     /locus_tag="ON396_002644"
     CDS             52..1851
                     /locus_tag="ON396_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HCR3114685.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(1919..3493)
                     /locus_tag="ON396_002645"
     CDS             complement(1919..3493)
                     /locus_tag="ON396_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR3114686.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         1038 a          695 c          750 g         1217 t
ORIGIN      
        1 taaaaaagtg ctatacttaa ttttgtatta caatatgaag ggagtttttt catgagttat
       61 tcattaaatt ggaatctaga ttctattttt tccggaggca gtcattctga tgctttgaac
      121 caacggatga agcaattgga agaccaaatg aacgagtatt atcaccgcgt aacaaaatgg
      181 tcacctagtt ctgacaatgc agagcagtta aacgctattc ttcaattgca agaaacgatt
      241 acgaatggtt ttacccaatg cagcagctat attactgcat tgctttctgc caatgtcaat
      301 gattcagatg caaaggtctt atcaggcaag ctttacgcaa tgctgcctcg tctgcaatca
      361 gccgaaactg tattgtcaaa gaaatttgca gaaatatctg acaatgattg gaatcagatg
      421 ctttctcaat cttcgtttga aacaattgct tttcgtttaa acgagattcg tcgtgatggc
      481 tcacaattat tatcagaagc tgaagaaaac attataaata cgctttcttt agatggtcta
      541 aatgcttgga gcagtcatta tgatacgatt gtagccagca tttcgattcc cttcgaacaa
      601 gatggacaag tagtcgaatt atctgctgga caagccttca ataagatgat gggcgatcca
      661 gatccaaaag tcagagaaac acttttcgca gcatgggaag aagcctggaa agaaaaggca
      721 cccctcttca cggatacttt gaatcattta gatggtttta gactttctga ctatgaactt
      781 catggtgtca cagattttct acaaaaacca ttagagtaca atcggttgaa gaaagaaaca
      841 ttgtctgtca tgtgggatac aatccagaaa aacaaacaac cccttgttaa ttacttgact
      901 agaaaagcta atcttttcgg taaagaaaaa atggaatggc aagatcaaga tgcacctatt
      961 attttaggcg atttgaaaga aaaaaccttt agttttgacg aagcagcagc atttattatc
     1021 gaaaacttta acaaattcag tccaaaaatg gctaaatttg cccaatctgc ttttgaaaag
     1081 agctggatcg aagcagaaga tcgtcctggg aaacgtcctg gtggttattg tactgagtta
     1141 ccagaaacaa aagaatcaag gatctttatg acctacagta attctgttaa tgaggttgcg
     1201 acacttgctc atgaactagg acatgctttc catagcagca cgatgtggga tttaccttct
     1261 ttaaatcgcg aatatgccat gaatgtcgca gaaacagcaa gtacattcgc agaactgatc
     1321 gtagccgatg ctacattaaa agtcgcaaaa acaaaagaag aaaaaatcaa tttgctagat
     1381 acaaaactgc aaaatgcttt agctatgttc atgaatatcc attcccgatt catttttgaa
     1441 aatcgattct atgaagcacg gcaagaagga ttagtctcag aagataagat cacagagatg
     1501 atggttgagg cgcaaaaaga aggctatcaa ggagctttag ctacttacca tccatatttc
     1561 tgggctgcaa aattgcattt ctttattgat gacgtaccgt tttataactt cccatacaca
     1621 tttggctacc tctttagttt gggcatctat gcttacgcca accaaaaggg agcaaatttt
     1681 gaagaagaat atatccattt actacgcgat actgcctcaa tgacgacgga agatcttgcg
     1741 aagaaacatt tgaatgtcga tcttacccaa ccagattttt ggcaagcagg tatcgacatg
     1801 gtgactgaag atatccaaac gttcatggaa ttgacagaag aatttatctg atttctattt
     1861 tatcacaata aaaaaagagt tagtccagaa tgggctaact ctttttgatt aatcattctt
     1921 acatgctgac agcaaagtca ggggcaaacc attgaactga accaactttt acgctttcgc
     1981 ctggttgagg agcatggata tattgtcctc cgcctaaagc aatcgctacg tggtaagttc
     2041 cgccttgtga cccccagaat aacaagtctc cagcttttgc ttgagaaact gaaatttttg
     2101 ttccagctga ttcttgagga actgtccaac caccgatgtc acgaccagtt acttgcatgt
     2161 aaacatagcg tgtgaaccct gagcagtcaa agccacttgg atctttaccg ccccaaacat
     2221 atggagtacc aatatatttg taagcttctg ctacgattgc tgcaccattc acacttccag
     2281 aaggagctgg agcaggtgtt acaggttttg gttgttcagg tgttggtgtt ggcgttggag
     2341 ccggagtact acttcctgtg ccgtttcctg tatcaacact ttgatctgtt gatggtgttg
     2401 taggcgcagg tgttgttgat tcttctgttg ttgaagattc aggcactgtt gttgattctt
     2461 ctgttgctga agattcaggc actgttgttg attcttctgt tgctgaagat tcaggcgctg
     2521 ttgttgattc ttctgttgct gaagattcag gcgctgttga ttcttctgtt gaagattcag
     2581 gtgttgttga ttcttctgtt gctgaagatt gtgtagctga tgatgattct tcagtcattg
     2641 aagattgtgt agctgatgat tcttctgttg ctgagctttg tgcagtagat gaagcttgag
     2701 tagcttgtgc ttcagcctct gcttgttcgc gtgcttcttt ttcagctttt tcttgtgcag
     2761 cttgttgacg agcttgttct gctagacgtt gttgttcacg aatacgagct tgctctgctt
     2821 cagcttctgc tttttgacgg ttcaagtcag ctttcttgtc ttcagcagtt gcttgttcag
     2881 cagctaatga agttttcagt acgttcaaat cagcttgttt agataaaaga tcgccttttt
     2941 gagattccaa agcagcttgg ttttcagcta attcttttag tttagcatcg ttttcagctt
     3001 ttttgtcttc tacagctttt ttatcttgtt tttgttgttc catcaagtcg ttgtttgctt
     3061 ttaccatcgt tgtcattgct tgaacgcgtc caatagcatc tgctaatgaa tcagcattca
     3121 atacagcatc gatgtagttt gagcttgtgt tgcttacttg tgcttcgcgt gcttgttttt
     3181 ggattgtatc ttcacgtttt tcaatacgtt cttgtaaatc tgcaatgtct tttactaatt
     3241 gtgcagattc ttgacgcaat gtatcttgtt ttgctaatag gtcttgtgct tgtgtgttga
     3301 tttcagaaac ttggctttct aaggcatcga tttgtgattg tgcatctgct tgttgatttt
     3361 tcaagtctgc aatcttttga tcttgctgtt gtatctgaga atcaaaatcg tccgccgcgg
     3421 cagcgatcgg tgatgctacg gcagtcaagg tcattgaaca taccattact gctgatatta
     3481 aactcttttt cactcttcat tcctccgact ggcttaattt aataaatatc taaatatatt
     3541 taagtctttc ttaacgatat aaaaattgta ccatagcagt gtgtcaacga tataatagtt
     3601 atgttacaaa aatgtttcaa aacaactttt aaaaatgaca aaactgttcg tttcagaaaa
     3661 tgatcatgaa aaagccgcgt taagtctagc atttcttaag
//
LOCUS       DAJSKS010000187         3688 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJSKS010000187 DAJSKS010000000
VERSION     DAJSKS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.195"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            58..510
                     /locus_tag="ON396_002646"
     CDS             58..510
                     /locus_tag="ON396_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114687.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            524..1213
                     /locus_tag="ON396_002647"
     CDS             524..1213
                     /locus_tag="ON396_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114688.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1338..>3688
                     /locus_tag="ON396_002648"
     CDS             1338..>3688
                     /locus_tag="ON396_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HCR3114689.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1256 a          479 c          769 g         1184 t
ORIGIN      
        1 caattatctt atcgtagata tatattataa atttaataag aaagcgaggt gagaatcgtg
       61 aaaaaaacga tactaagtat tttttcagta tttacaatat taattggggg ctattttctt
      121 ttgaatttag attctgttaa ttcaaacgct caaataaaag atcaaagagt agctgaacat
      181 aataataaaa aaatgagttt gaccaaaaat atctattcta tgtcagatga agaattgtat
      241 aaagaatttg gtgatttatc cggggaacag ttagaagaaa gtattccagg aattagtgac
      301 tcagataaaa ttgttgtctt gtctgctggt gggcaattaa tgggagatgg caaatccgaa
      361 ttagttagtt ataaacaaaa tcacagacag tttattgatg ttaatactga ttccaacgct
      421 atttcgatta aggatctaaa agagctttta aatagtaaaa gaaatgaatt aaagcagata
      481 aaagaaacta attcagtgga attagagtaa aggaagattc attatgaaaa agatttttgg
      541 tgtttctgtt gggctactta tgatgttaac atttggaaat gcagtatatg ctgctgagaa
      601 agaagttatt tcagatccag agcagtacgc ctcacgatac tttgctgatt ttgaagaaga
      661 agataaagta cttgcattgc cagaaggtgg ttttttaaaa ggggaagcta aaattgtatc
      721 tatggataat ccagatataa ttcaaggttc atataattca gaaactgatc ctgcttcagt
      781 ttcagtgaca gaagcaaaaa aaattgttgc tgaaaaattc aaaaatgata ttgaaactac
      841 tccaattatg cctcgaggag catttccacc cgatgttggt actagcggac accttcttgt
      901 actagcagga ggaggaagct ataaatcaca gccatttagt ggtgcgggat ggcgttttgg
      961 aggtatgggt gtaaaaccag ctgatggtac aggcaacttt ttacgttgga aatcctacat
     1021 tgacgatgga agagtaggaa atgtaaatca agccaacgca actaaatctg gttctattca
     1081 aggaatggca ttgtatgctc gtgaaggatt caaatacttt gcaccaaacg ggcctggttg
     1141 gacaatgaat ataggtattt attacactta taatcctgtt gcaggtacgt attatgttgt
     1201 tcaaaatatt taataaaaca gcacaaagag atttctttgt gctgttttat tatttttagt
     1261 tgttgacaaa cactaaaata taaaatataa tcaacacata aacgaacgta tgttcggcaa
     1321 gaaagcaggg gatttttatg gatgagatgg ttttaagtac tcagaaatgg ctaaacaaga
     1381 aatacagtaa tgttacaggg tttgataagg ttccagaaaa tgggcgaaca gggtggccta
     1441 caatctatgg acttattgaa ggactacaag tagaacttgg tattactaat ctagtagcta
     1501 attttggacc taccactgaa aaaatgtacg ataatcaggt tactcctaaa tggggcaaaa
     1561 atctacctaa aaatattgta tttcttattc aaggtgcctt ttggtgtaag gggattaatc
     1621 caggtggctt cgatggggta tatactccat atttagatac tgctgtaaaa gagttgcaga
     1681 ctgatgcagg gtttaatgga agcgctgtaa ctggagtttt ggatgcgaaa tgggccaaag
     1741 cgttatttga tatgagtgct tttgttcttg ttcccggcgg agatagtaaa attagaaaaa
     1801 tgcagcaatg gttaaatgta aattatttag aatacacggg aataatgcct tgtgatggta
     1861 tttatcaaag aaattcaaat caagcattaa tttttgcatt acaagctgaa ctaggatatt
     1921 ctcccagtga agcaacaggg tcttatggta atgggacaac tagtaaaact tatcctgtgt
     1981 ctgaaggtaa ctcgggaaac tatgtaagaa taattcaata tggattatat gttaatggtt
     2041 attttgaaga cggtacattc gatggtattt tcactaaata tatgggttta gaagtactgg
     2101 cattccgaaa atttatggta ttgcctgaat atactgaaat agcagatgtt gttgtaataa
     2161 aaggactatt gtctagcgct ggagatatta gaagatctgc agatggcgca gatacttcta
     2221 cgcaattaac tagatctcaa atccaaacat tggttgataa tgatataaaa atagttggta
     2281 gatatcttac tggtactgtt ggaattggaa aagatgaacg aaataagtat ctaactacag
     2341 aggaattgaa taatattttt tctaaaaatt tatctgtgtt tcctatctat caagatggag
     2401 gtgcagcatt agcttatttt acttatgatc gtggcttatc agatggtaag aaggctatag
     2461 atgcagcaaa aaatctaaat ataccattaa caacagttat ttattttgct gttgatctag
     2521 acatgttagg ggaagaaatt cttgcatatg ctggagaata ttttaagggg gtatctgctg
     2581 taatgtcata tagcgggtat caaacgggtg tttacggaac aagaaatgtt tgttctcaaa
     2641 taatcaataa cgggtatgct tcgtttgcat ttgtttcaga tatgtcgact ggttatagtg
     2701 ggaatttagg ttttcctatg cctagacaat ggtcttttga tcaatttgtt gaatttacca
     2761 taggaagtgg caacggagct gttggtatcg atttaatcgc tacatcgggt agagatagcg
     2821 gatttaatga gttatcaaat gatactaata atgataatta cattgccaaa tacaatcaga
     2881 aggttataaa tcagatggta cgtgcgtatc aatatctatc acaagctggt cttgataatc
     2941 cttggaaccc tcatttgaca ttttatagat atgtaaatta ttctggtttg tcatgggaca
     3001 tcatttcttc tcctgtaaca gagcatgata gaaaaattta tgatgaatat aggaataaat
     3061 taactaatag cgagggcctg tataattatt ttatagatcc taatactggt tcaataatag
     3121 gtttgcctca cttaattgtg acgttacaat cacagatgtt tctagccgac acaatcaatg
     3181 atagtgtttc tgactttgca ggttggttag gagatttaat gacttgctgg ggtgaagtta
     3241 agaaattagg aatacaaatg gagcaaggtg tatttgcatt agtaggtacg gctgcaggtg
     3301 gaacatttag tctagaagat ttataccaag atgttgacgc ggtaaatgtt tataccttag
     3361 caaaaagaaa ttctgattca acagctttat cggctttaaa aacgtactat tgtgataagg
     3421 gtgctattga tagatttaat caatttgtta agaacagatt tggttcttca gataaaatcg
     3481 cttcagtaac aaatagttta ttagaaaatg acgaaacagt tgatatctta ggtgtaggga
     3541 taatttcatt tttcattgat aaaatagtta ataattcact tgggacagat tttacttatc
     3601 ttgattttat tggagatgag gggaaagttg caaatggatt ttctgaaaaa attattcaat
     3661 ttcaagaaaa tagaagtaaa cttaatta
//
LOCUS       DAJSKS010000188         3602 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJSKS010000188 DAJSKS010000000
VERSION     DAJSKS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.196"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..186
                     /locus_tag="ON396_002649"
     CDS             <1..186
                     /locus_tag="ON396_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3114690.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
     gene            329..553
                     /locus_tag="ON396_002650"
     CDS             329..553
                     /locus_tag="ON396_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3114691.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            568..1437
                     /locus_tag="ON396_002651"
     CDS             568..1437
                     /locus_tag="ON396_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCR3114692.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1418..2152
                     /locus_tag="ON396_002652"
     CDS             1418..2152
                     /locus_tag="ON396_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3114693.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2185..3093
                     /locus_tag="ON396_002653"
     CDS             2185..3093
                     /locus_tag="ON396_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HCR3114694.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3090..3570
                     /locus_tag="ON396_002654"
                     /pseudo
     CDS             3090..3570
                     /locus_tag="ON396_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1251 a          521 c          789 g         1041 t
ORIGIN      
        1 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
       61 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
      121 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
      181 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
      241 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat ggtttataat
      301 attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta aagaactgtg
      361 tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga ctccacagaa
      421 tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg ccattgctga
      481 tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag aaataaaaat
      541 atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat agtaacagag
      601 aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg tgcaagaggc
      661 acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga ctcatttgac
      721 ctgacagcga ttaatcaaat tgctacagaa ttggatgata aaaatagaaa caaccttgtt
      781 gtgcctcccg gagcgtgggg tgattggatt aatggcggcg gatggttatt tataaatggg
      841 tatcatgttg acttgatttt acgtgatata aaacgggtag aacaaataat caaagatacg
      901 gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta tataagtgca
      961 atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga aagcttatgc
     1021 gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt gatgaacttt
     1081 tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg ggtagaggat
     1141 aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca agtactattt
     1201 gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct tgaaactttt
     1261 gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt actcggtctt
     1321 tctctttttg aatgctacga tatgaccgag aaactttata aagaagtgaa tgaaattgta
     1381 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga
     1441 taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc
     1501 gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga
     1561 tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt
     1621 agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc
     1681 acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga
     1741 tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa
     1801 gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt
     1861 tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc
     1921 ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac
     1981 aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata gttttataat
     2041 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
     2101 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
     2161 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
     2221 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
     2281 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
     2341 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
     2401 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
     2461 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
     2521 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
     2581 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
     2641 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
     2701 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
     2761 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
     2821 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
     2881 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
     2941 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
     3001 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     3061 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     3121 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     3181 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     3241 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     3301 ataagataat atatctttta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     3361 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     3421 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     3481 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     3541 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     3601 tt
//
LOCUS       DAJSKS010000189         3559 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJSKS010000189 DAJSKS010000000
VERSION     DAJSKS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.197"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            4..1770
                     /locus_tag="ON396_002655"
     CDS             4..1770
                     /locus_tag="ON396_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HCR3114695.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            1793..>3559
                     /gene="uidA"
                     /locus_tag="ON396_002656"
     CDS             1793..>3559
                     /gene="uidA"
                     /locus_tag="ON396_002656"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR3114696.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQ"
BASE COUNT         1283 a          445 c          731 g         1100 t
ORIGIN      
        1 aaaatgacta taaaagtaga tttacgaaaa aaaccatata atttagatga agatgctatt
       61 tcttggattg aagagacgat tgattctatg actttggaag aaaaaatagg tcaattgttt
      121 gttaatatgg gatctagtcg tgataaaaat tacttgagta atatggtcaa tcagtaccat
      181 attggagctg tacgatataa tccaggaact gctgatgagg tatatgatca aaactatatc
      241 ttacaaacag aaagtaaaat tcctctatta attgctgcta atacggaagc aggtggtaac
      301 ggagcttgta cagatggtac tgaagttggt gtagaagtaa aaattggtgc aacaaatgat
      361 gcgaaatatg cttatgaatt agggagagtt tctgggattg aagcatcggc tattggatgt
      421 aactggagtt ttgctcctat tgtagatatt aatagaaatt ggagaaatcc tatcatttct
      481 agtcgatctt ttggacaaaa ccctgataaa gttttagaac ttagtttagc ttatatgaaa
      541 ggtattcagg agagtggaat tttgcctgca gctaaacatt tccctggtga cggtattgac
      601 gaacgagatc agcatctatc ttttagtatt aatagtttag gtacggaaga atgggataat
      661 tcttttggaa aagtatactc aggcttaata gaagctggat taccttcaat tatggcaggt
      721 catatccata tgccagctta ttcaaaacat tttaatcctg atctgaaaga tgaagattta
      781 cttcctgcaa ctttatcaaa agaattaatc actgacttac ttagaggaaa actcggattt
      841 aatggattag tagttactga tgctagtcat atgttaggaa tgactggggc aatgaaacga
      901 tcagaattat tgcccacttc tattgcagct ggatgtgatc tatttctatt ttttaatgat
      961 ccagatgaag actttaatta tatgatggaa ggctatcgta aaggaattat cactgatgat
     1021 cggttattag atgccttaaa aagaatttta gggttaaaag ctaaattggg attacacaaa
     1081 aaagcaaaag aacaaatttt attacctaag caagaagctc tggaaaaaat tggtttaatt
     1141 gaaaataaat caatttttaa agaggtggct gaccaagcaa ttacattagt taaagataaa
     1201 caagatattt ggcctattgt accagaaaaa tataaaagga ttttactagt agatgtacat
     1261 ggggtaaaag gtgggtttgg tgaagtagtt gctggaaata gacctcaagc cattgattta
     1321 ttgaaacaga ttttgtcagc aaaaggattt gaagttgaaa aatacatttc acctgaagaa
     1381 gaattgaaag gattatcgca agaagaaaaa attaaaaata tagctggaat atatgctgct
     1441 aagagaccaa ttaaaaattt aactaaaaaa tatgacttga tcattaatat agctaatgtt
     1501 aatccaggca cagttcaacg aattgtctgg ccagcaagta aaggaactcc ggatataccg
     1561 ttttacattc atgaaattcc aacaattttt gtatctgtac agtgtccgtt tcatttggct
     1621 gatgtacctc aagtaaagac ttatattaat acttatgatg gaaaacaaga aactttagaa
     1681 atattggtgg acaaactaat gggtctttct gaatttaagg gagtaagtcc aatagatgct
     1741 tattgtggtt taaaggatac atttatttaa tatttagata ggagtggttt atatgttgta
     1801 tcccattatg acagagacga ggcaagtaat tgacttaagc ggtctctgga gatttaaatt
     1861 agatacaaat gatgacttac aggaagaact tgctttagaa ccaataaatg aaaaagagag
     1921 ttatccgatt tcagttccct cttcttataa tgatttattt gaaacagaga acattagaaa
     1981 tcatgttgga tgggtgtggt atgaaaaaga agtgactctt cctaaaagat tattagaaga
     2041 aagattagtt cttcgttttg gatctgcaac acatgaagca aaagtttttt tgaatggaaa
     2101 gtttattact caacacaaag gtgggtttac accatttgag tctgaaatta atcggtttgt
     2161 aaaagacggg aataatcgaa ttacagttgc tgtaaacaat attattgacg atagtacttt
     2221 acctgtgggg attatgaagg aaacagtaca ggaaaatggg gatataaaaa aggaaaactt
     2281 agttaatttt gatttcttca attatgcagg tattcataga cctgttaagt tatatacaac
     2341 tccgaaagac tatataagtg atattactat taatacaaga ataactgatt tggatgcaag
     2401 tgtagaattc aatatcgata cagaatggac cggaaaaatc ttaatagcga tatatgacga
     2461 agaggctcaa cttgtagctg aagctgaagg aaagaaagga atgattcagc ttaagaatcc
     2521 aattttttgg gaacctctag acgcttattt atatagtatg aaaattagtt taattgatga
     2581 taataaagtg atcgatacct atacagaaga atttggtatt cgcagtgttc aggttaccaa
     2641 tggcaaattt ttgattaata ataaaccttt ttattttaaa ggatttggta agcatgagga
     2701 ttcgtatgtt aatggacgtg gtttgaatga ggctgtaaat attaaagatt ttaatttaat
     2761 gaaatggata ggtgctaatt cttttagaac atcacattat ccttactcag aagaaattat
     2821 gcgattagca gatcgagaag gtattgtagt tattgatgaa gtacctgcag tagggttaca
     2881 tttaaatttc atggccactt tattagatga taatgtggaa aaacataata cgtggaaaga
     2941 gatacgaaca gaaaattctc ataaaacggt gatcaaggaa ttgatagaac gcgacaagaa
     3001 ccacccaagt gttgtaatgt ggtctattgc aaatgaacca gattcggatt ccgaaggtgc
     3061 taaagaatat tttgaacctt tagttgaatt aactaaagaa cttgatccgc agaaaagacc
     3121 tgtaacaatc gttacttatc taaaatcaac gccagatgtt tgtaaggtag gagatattgt
     3181 cgatgtttta tgtttaaatc gttattatgg ttggtatgtt tcgggagggg atctggcaca
     3241 tgcaaaatat ctgttgaaaa aagagctaga tggatggatg gaaagatgtc ctgatacgcc
     3301 aataatgttt acagaatatg gagcagatac agtagctgga tttcatgata cagtaccaac
     3361 aatgtttaca gaagagtatc aagtggctta ttacgaagca aatcacgaag taatagatac
     3421 atgtgaaaat tttgttggtg aacaagtgtg gaattttgca gattttgcaa caagtcaagg
     3481 gattttaaga gtgcaaggaa ataagaaagg aatctttact agagatagaa aacctaaaat
     3541 gattgcttac tatctacag
//
LOCUS       DAJSKS010000190         3474 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJSKS010000190 DAJSKS010000000
VERSION     DAJSKS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.198"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..675
                     /locus_tag="ON396_002657"
     CDS             <1..675
                     /locus_tag="ON396_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HCR3114697.1"
                     /translation="YEFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILE
                     LLDGYNSDLISEYKNEIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKL
                     SHKSYKDVYDEYSKETDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEH
                     RGELIKNLVTNRAKIQTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVK
                     SYLKMN"
     gene            678..1574
                     /locus_tag="ON396_002658"
     CDS             678..1574
                     /locus_tag="ON396_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR3114698.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            1624..2013
                     /locus_tag="ON396_002659"
     CDS             1624..2013
                     /locus_tag="ON396_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR3114699.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            2000..>3474
                     /locus_tag="ON396_002660"
     CDS             2000..>3474
                     /locus_tag="ON396_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3114700.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPLSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGG"
BASE COUNT         1233 a          515 c          709 g         1017 t
ORIGIN      
        1 tatgaattta cacaagagaa tggtctggtt gaaaaagaaa cagaacggtt gagaatgatt
       61 tccgataagg tgagtatata ctctcaaaaa caaatttatg aaatttcaaa aaatcaaact
      121 agtatactag aattactaga tggatataac tctgatttga tttcagagta taaaaatgag
      181 attgaaacat gtgtaaacga aataagaaaa ttaaattggg atattgtttc tgtgaaaaaa
      241 gaaatacaag ataaggcgaa ggtagagctt gaaattgaag atttaaaaaa acaagtagaa
      301 aagctatctc ataagagtta taaagatgtt tatgatgagt attcaaaaga gacagatatt
      361 tatagggaac tgaaacgtga tgtggaggca cttaaggaaa ttcccaaaaa tctaactgat
      421 agtgttgaca aaattgactt tggcaatggt ataaaagtta cagacgaaat tgatgaacac
      481 cgcggagaat tgattaagaa tttagtgacc aatcgggcta agattcaaac aataattaat
      541 gaaatgtcag atgaaattga tagttataga gcagagctga ataaatcaga ttggaaagtt
      601 aactataatg aggtttcaaa gaggtatcaa agaggtatca aaaacttagt gaagagttat
      661 ctcaagatga attaaaaatg ttacaaaacc ttgattcttt atatcaatct ttatcaaaaa
      721 ctgaagagcg attggaagaa attattcaac aagaaataaa gcttaaagat taccaagttg
      781 aggttgactc agcaatgtca agaatgaata aatcatatca aaatttctat tctgagagaa
      841 gtagttcctc gcgcgaaatt ttttctgata ttacaggaat agaggctact attcaaccaa
      901 atagggattt caataattat gttaaagagc tcagagcttg tattaataag gaggacactt
      961 ttgacagtga gtttggatct cttattgata agctgacaaa aaattccatt actaatcaag
     1021 atatatataa tgacatactt gatataagag ttagtgggga aagtagtatt ttttcagata
     1081 aaaaattaat caattctata agaaaattga atgatgcaca agttcttgaa ttacaaaccc
     1141 ttaagccaaa tgatttagta gttatccgac ttgaactaaa tggaaaaaaa gttagtttat
     1201 cgaatgctag tgctggtcaa aagacatctg caattctttc tatgattttg gcgtatggaa
     1261 attcaccact catattggat cagcctgaag atgatttgga tagtcaatta attaataact
     1321 taatcgtaag aagtatcatt acaaaaaagg aaaacagaca aattttaatt gtaactcata
     1381 atgcaaatat cccagttaat ggtgattcag aatggttgat tagcatggga gattcaaaag
     1441 aaatatcggc tgatcagtgc gggtcggtag atgaacccaa tatcaagcca agaatatgta
     1501 ctgtgatgga aggtggcgag gatgctttta caaatagagc gaaacgctat ggttttaaac
     1561 aaattgtaga ataacactag agggtgagac atattattat gtccgccctt tttaggagga
     1621 aaaatgaaaa aaataaaaag ctataccagt atctgggcag ttgaaaaagt catttacgca
     1681 atcaatgatt ttcagttgcc ttttcctgtg acttttaacc aaatggcttg gttcgtttta
     1741 tctttactgt ttgtcatcgt atttgcccat gtgcctccac tatctatgat agaggaggtc
     1801 tttttaaaat acttaggcat accggtggct gtcacttggt ttatgtcgca aaagacgttt
     1861 gatggcaaga aaccactggg atttttacga tcctttatca gctatcactt gcgatttaag
     1921 gtgacttttg caggaaagaa agtcaaagaa cagaagaaac gatgggatga acccagtact
     1981 cttgtaagga gtgtgaacta tgtatccgat taagtatatt gaaaataatc tcgtctttaa
     2041 ccaagaaggc gagtgttttg cctactatga attagtgcct tacaattact cctttttgtc
     2101 acccgaacag aaatttcaag tgcatgataa ctttcgccaa ctaattgcgc aaaatcgtga
     2161 aggcaagatc catgcgttac aaattgccac agaaagtagt attcgcgcca cacaagaacg
     2221 atcaaaaaaa gaaattaccg gtagactcaa agaagtagcg aaacaacgaa ttgatataca
     2281 gacagatgca ttagtatcca tgattggcga tagtcagatt gattatcgct tttttattgg
     2341 ctttaaacta attgcgacag atgaagaagt caatctaaag agtctcaaga aatctttctt
     2401 ttctgggctt caagaatttg tctatggagt gaatcatcat ttgatgggcg attttgtttc
     2461 cttatccaat gaagaaattc gctgctattc taagcttgaa aaattaatgg aaagcaaatt
     2521 ggctcgaaga tttaaagtca ggcgagtcac accgagtgac ctgacgtatt tgattgaaca
     2581 tatttatggc gaaaaaggga taccgtttga agaatacgaa tttcagctac caaagaaaaa
     2641 gctaaaatca gaaacactgg tgaaacgtta tgacttatta cgtccaaatc gttgtttaat
     2701 tgaagaaaaa ccaaggtgct tacggatgga acatgagaat catgagtcgt acgtagcgta
     2761 tttaacaatt aatacaattg tgggggagat ggaatttcct tcctcagaac ttttctatta
     2821 ccagcaacaa caatttacat tcccaatcga cacaagtatg aatgtagaga ttgtgacgaa
     2881 taaaaaggct ttagctaccg ttcgtaataa gaaaaaagaa ctgaaagatt tagacaatca
     2941 tgcttaccaa tctgacaatg aaacgaattc caatgtctta gatgctttgg attccgtgga
     3001 tgaattggaa accaccttgg atcagtcaaa agaatctatg tacaaactga gttatgtggt
     3061 tcgagtgagt gccgaatcag tggatgaact aaagcgacgc tgtgatgaag tgctcgattt
     3121 ttatgatgat accaatgtaa aactagtccg gccctttggc gatatgatgg ggctacatga
     3181 agaattctta cccttaagta aacgatatat gaacgactat attcagtatg ttacgtctga
     3241 ttttttggcg gggcttggtt ttggggctac tcagatgcta ggagaattag aagggattta
     3301 ctttggctac aatgtggata caggtcgtaa tgtttacctg aaaccggcat tggcttcaca
     3361 aggagtcaag ggttcagtaa ccaatgcgct ggccgccgct ttcctgggtt ctcttggtgg
     3421 aggtaaatcc tttagtaaca atctcttagt ctactatgct gttttgttcg gtgg
//
LOCUS       DAJSKS010000191         3383 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJSKS010000191 DAJSKS010000000
VERSION     DAJSKS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.199"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..188
                     /locus_tag="ON396_002661"
     CDS             <1..188
                     /locus_tag="ON396_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR3114701.1"
                     /translation="WSSIVYGGKIAFYFSAIIEFLQLFLRLGTFQISDFVYNTLGGIL
                     GVILYKLILITKRDGER"
     gene            185..715
                     /locus_tag="ON396_002662"
     CDS             185..715
                     /locus_tag="ON396_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HCR3114702.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1581..2540)
                     /locus_tag="ON396_002663"
     CDS             complement(1581..2540)
                     /locus_tag="ON396_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HCR3114703.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSIDFRE
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            3102..>3383
                     /locus_tag="ON396_002664"
     rRNA            3102..>3383
                     /locus_tag="ON396_002664"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1153 a          498 c          667 g         1065 t
ORIGIN      
        1 tatggtcctc aatagtatat ggaggaaaaa ttgcatttta tttttcagca ataattgaat
       61 ttttacaact ttttttgagg ctaggaacgt ttcaaatttc ggattttgta tataatacgt
      121 tgggcggtat attgggtgta atcctttata aactaatttt gattacaaaa agagatggtg
      181 aaagatgaga ctcggattga taataaagaa tatattaaat ttacctaaaa cgatatactt
      241 caattataaa tttcttcata tttcagatgc gtggagatta cctattttgg tgcatcgtaa
      301 agtacggatt acacgcaaat cgagagggaa aattgagata gtattacaat ctggaaaaat
      361 agtttatttg ggctttccag tattaaatac tgcgggaaat gaagaaacga ttataagtat
      421 tgaaggaata ctaagaatac aagggccagc gtttatcgca caaggggctc atattgacat
      481 aaggaaaaat gcagttttag atattgggtc aaagtttaat tcgacaggaa aaatgagtat
      541 aatttgcttt aaggaaattc aattcaaaga tggctgtaca gtatcgtggg atacattatt
      601 tatggatacc gattcacata cgatttttga attgaatact cataggattt tgaataaaaa
      661 taataaaata gttataggta aacattgttg gattggagca aaaagcgtga tatagaaagg
      721 tgttcattta gcagataata ttgttgttgc ttgtaattcc acggtagtaa aaagtcatga
      781 aaataataat gagattatag ggggggctcc agttcgaacg ttgaaaagaa atacaagttg
      841 gtgtttgaac gagccaaagt gaaaccaaat aattataaaa ctatggaagt ataataaagt
      901 ttttgagaaa tttgaacgac aagaccctaa aaaaatccat ggaccattat tatggatgga
      961 cttcccaaaa ggaatagcca cttttgagca cgtaaatccc ctaataagtg gatcgacatc
     1021 gcttgtgcag cagatgatac tatcaagaaa ggtgtttata atgtagaact tgaactaaat
     1081 agatttgtaa tcacccatta ggattgtaaa gaatatgaat aggatatatc ttaagatgaa
     1141 aaaatttgca aattctaatg tatatttatt aagggcactt tgctgtgttt ttttcagaga
     1201 taagatgaaa gaaagatgga agtcttggag tatacgaaat aaagaaaaat attatatctg
     1261 tttaataagt tgatattgaa caaaggacta gtcggagtca gcaacacata agttgtgagt
     1321 gatgctgact cgtttttggc agaaaagaat cgaatagtaa gtaggtgttt tgatgttgct
     1381 tgacaatgga gagagaaccc aagaagatta ctagcaaagc ttatagatgt tactgtagat
     1441 ttttaactcc agatacagag tttgatgaat attctgatta tatcaataac actataaaaa
     1501 aggaaatttc cttttcacag gagaaaggta aaagatattt tcataggaga atcaatgtta
     1561 attttaaatt atatgatttg tcaaattaag ctagaataaa atgcttcttg tacatagctc
     1621 aaaaatattt cgattggtgt tctgtaattc aatgattttc ttggaatatg attgcgttga
     1681 ttgctgacac tggaaataaa tgtctgattc acttctctaa aatctattga tttcggcagt
     1741 ccattacgac gcagaatccc gttagaattt tcgtttaatg ggcgttgaga aggtgttcca
     1801 gggtccgcaa aatatatatc aatatcatgt tggttactaa tggctttcca gttagaaaat
     1861 tcttttccac agtcaaacgt aatagattta aagaagtttt tagggaagcg agaaaaccat
     1921 tgattaaggg cagtttcaat atctaatgcc ttacggccgt tgggtttaat cgtgataatg
     1981 actttagata atctttcaac taaagtaatg acggcacttt tatgatgaat gccaacgatc
     2041 gtatcacctt caaggtgacc aaactcagaa ttgaaatcag gataattatc aggtcgatca
     2101 tggattgagc gctgatactg ttgttttccc cgtttttcct gatggccatt gggttttctt
     2161 ttacctttca tcggtagtgt gtcaatatca aatattcctt tagaaaataa acgataaagt
     2221 gttctcatac cgcatgaaac aggcctttct tttcgcccga taatgacgtc aggcgtccaa
     2281 ccaagagtga ctttctcttt aatgtaatct acttcatgag caggtaattg aatgactttt
     2341 ctaccacatt ttttcttatt ttctttatac tggtgccaat aatcaagagc agtcttgcct
     2401 tgcttgaact tattgactac attataaata gtttgtatag cacgtcccat tcggttagcg
     2461 atttcaaccg gcttattatg ttgaagataa tatgattcta tcattgtcag ttcgtctatg
     2521 gtaagatgtt tgtaggtcat ttatggttac actcctttgt tttctttcgt cggaaataca
     2581 atttgagtgt accataaatg cctttttatt tttctaactt aattttacaa ttcgcgatat
     2641 attaaacaac gttgtcgcgc ttcattgaaa cattttatga aaattccaaa aaagagttga
     2701 cacatgtttt tagacgtgat aagataacta agtcgttaaa aagaaccact tgcgaagctt
     2761 gattctatcg aaaaaaaact tcaaaaaagt tcttgacaaa taacaaacga taagttatga
     2821 taaatgagtt gctgaaagat ttcgaaaaac gaaatcgaaa aaaactttta aaaaagagtt
     2881 gacaatcgct tgtctctctg atatgatata aaagttgcta cggcagccaa aaacgaatcg
     2941 ggaaacactc ccgatgaagt agatctttga aaactgaaca aagtaagaca aaccaaatgt
     3001 gtagggcgtc ttgattcaat tcaagacaat aaacattttt aacaagcaag caatatgcta
     3061 gcaaacaaat tgagcttaac aatcgcaaga ttgttcgaac tttttatgag agtttgatcc
     3121 tggctcagga cgaacgctgg cggcgtgcct aatacatgca agtcgaacgc ttctttttcc
     3181 accggagctt gctccaccgg aaaaagagga gtggcgaacg ggtgagtaac acgtgggtaa
     3241 cctgcccatc agaaggggat aacacttgga aacaggtgct aataccgtat aacaatcgaa
     3301 accgcatggt tttgatttga aaggcgcttt cgggtgtcac tgatggatgg acccgcggtg
     3361 cattagctag ttggtgaggt aac
//
LOCUS       DAJSKS010000192         3368 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJSKS010000192 DAJSKS010000000
VERSION     DAJSKS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.200"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..1157)
                     /locus_tag="ON396_002665"
     CDS             complement(<1..1157)
                     /locus_tag="ON396_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3114704.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
     gene            complement(1160..>3368)
                     /locus_tag="ON396_002666"
     CDS             complement(1160..>3368)
                     /locus_tag="ON396_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR3114705.1"
                     /translation="NQQFHLFNQEISYIFKISEDGKLLHLYYGENLPDNDYSHLIEMH
                     HRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNGSRLSDFVYQGYEVE
                     EGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYRDYPVITRSARVMNK
                     GKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQGIQSIESTRGISSH
                     QHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTRLQVGINPFGFNWKL
                     NPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKDRPVLINNWEATYFD
                     FDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVNPKRLPRGIGSLSEK
                     IRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRNQYVLNFGLDEVVEN
                     IFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHRYVLGVYRLYEKLIT
                     KYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQYGTSMLYPLSSIGAH
                     VSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIVKEQIAFYKKYRTII
                     HNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAPYRRLKLKGLDNDGL
                     YNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSKIWVITQEEK"
BASE COUNT         1094 a          631 c          488 g         1155 t
ORIGIN      
        1 actctagtat tttttttatt tgtcgcaaaa aatgttacaa ataggatcac aataaatatt
       61 cctgcgaata caaaaaatgg agcagtcaaa tttgtacttc ttgttagaaa ttgtgctaat
      121 tgcatagcaa taggagctat aaaattacca atattacagc caataaaaat caatgaagta
      181 gcaaaattaa ttgtattctt tgtagtgact ttatctaagt tactaaagat aaatgggaag
      241 caccatgatc ctgttatact tgataaaaat aaacccgtta gcaggaacgt catattaccg
      301 tttgacacag caattaacaa atttgaaatt ataaagaata taattcccaa gtatagggtt
      361 ccttttccta tccacttatt tattggtcca aacataaatc ctgctagtat tcctaagata
      421 ggcattagag ctaaatagtt actagcatta aagtttaccc ctttaatttc agttgcaatt
      481 gatgcaaatc gtacagaaat agcaatacta ttcattacga gtaagattgc aaatagaact
      541 agggcaaaaa ccattggatt catcttttct tttacatgat tttcaggtat gtcactattt
      601 tcatctctaa tttcaggaac ttttagcgcg aataagagag ctacaggaaa cgcaattgca
      661 tagactaaaa acgaagccgt ccaattaatg ttcactaaaa ctccagcaag aaaaataagg
      721 attgtctgac ctaaagcttc cattgaattt ctaataccta atagcgttgc tctagtattg
      781 ccagagtata aagagctgat atatgtcaca gccaatgaat tatatactcc taaaccggca
      841 cctaatatta ctcgactaat aagaacggtt tggaaggtag ctgtaaatac tggaataatt
      901 cctcctatac cagctaataa taaacctaaa attattgtct ttttcattcc tattcttttg
      961 gcaaagtaac tagataataa gataaacaaa ataacagtta tacttggtgc agttcctaaa
     1021 agttcacttt gactttgtga tatccccaaa gactgtttca tttgtggaat aacaccatta
     1081 attgcttgag agcttgttaa cattagtgaa atacttaaga ttgctccttt ttgaagtaag
     1141 cttttttggt tattcatctc tacttttcct cctgtgttat aacccaaatc ttggaatcaa
     1201 aatcaaatgt ttctgggcgt cgattctgat catttatttg tccactagat gcatctgaac
     1261 agactagtcc tgctctcatc aattccattc cagatcgcaa actattttgt cctacattat
     1321 aaagtccgtc attatctaac ccttttaatt tcagtcggcg gtaaggagca tttacctcat
     1381 ttaatgtttt ataatgagca acaatagccg ttttcttatc atgactaaca accatccacg
     1441 cggtttgatt atcatcatcg aatggtgata atagtctata aaaagtacca ttatgaatta
     1501 tggttctata ttttttataa aaggcaattt gttcttttac aatcctcaaa tcgccttcac
     1561 ttaattcatt taagtcaagc tcatatccaa atgcaccaaa aaaagctaca tttcctctag
     1621 tttttatagg tgttatacgg tttgtttgat gatttggaac aattgataca tgagcaccaa
     1681 tagacgaaag aggatacagc atactagttc catattgaat ttttaacctt tcaattgcat
     1741 ctgaatcatc actagcccaa gcttgaggtg catagtgaag cattccagga tcaaagcgcc
     1801 caccacctga tgaacaagac tcaaatagta tgttaggata ttttgttata agtttttcat
     1861 ataagcgata tacgcctagt acatatctgt ggaatacttc tccttgttga tctgctgaca
     1921 agtgactgtc aaatacatca gtcaatggac gattcatatc ccacttaatg taatctaagt
     1981 ttgattcatc aatgatttta cacatttgat cgaaaatatt ttcaacaacc tcatctaacc
     2041 caaaatttaa tacatattgg ttcctaccat gattaggttt tcgattaggt gtagaaataa
     2101 tccaattcgg atgtgtgcga tataactcac tatccttatt caccatttca ggttcaaacc
     2161 ataaaccaaa tttcatacca aaactacgaa ttttttctga aaggctgccg attcctctag
     2221 gtagtctttt aggatttaca tgccaatctc ctaatcctgc acaatcgttt tctctttctc
     2281 caaaccagcc atcatctaat acaaataatt caataccaac atcaaatgct tttttagcaa
     2341 tatttaccaa cttttcttcg tcaaaatcaa aatatgttgc ttcccaatta ttaattaaga
     2401 caggacggtc tttttctcgc cacttaccac gagctaagcg ttttctaaat agttgatgaa
     2461 aagtttgact catggcattt aaacccttac ttgaataaac cagaatcgct tctggtgctg
     2521 taaatatttc tcctggattg agtttccaat taaatccaaa tggattgatg ccaacttgta
     2581 gtctagtaac atcccatgta tctacttctg cttgaattaa gaagtttcct gagtaaacta
     2641 gtgctgcacc aatagcttct ccttgatttt catcagtatt ttttcttttt aaagtaacaa
     2701 atgggttatg ctgatggctt gaaatacctc gagtagattc gattgattga attccttgtt
     2761 gtaaagtacg cgacttaata gttctttctc taccccaagc accagataat tgttgccatt
     2821 cataatttgc atcgggtaaa tccaatgata aactgctaat attctcgatt atttgaatat
     2881 cttttccttt attcataact cgcgcgctac gagtaataac aggataatct ctataaattg
     2941 tatataataa ttctacttga acatttgtaa ctgaatctaa aatataaact ttcaatgttt
     3001 tgctttcatc attattatca acataggtag caggtaaacc ttctagttta ggttttcctt
     3061 cttccacttc gtacccttga tagacaaaat ctgataagcg agagccattt ttctgtagta
     3121 atgaaatagc aggatgtcta aaatctgttg taccatattc aggaaattct tgttttaagt
     3181 gctctaacga atatagcaaa tcgttttcat ttctataggt tgtcataggc cgatgatgca
     3241 tctcaattag atgactatag tcattatctg gtaagttctc accataatat aaatgcaata
     3301 atttaccatc ttccgatatc ttaaaaatgt aacttatctc ctgattaaac aaatgaaatt
     3361 gttggttt
//
LOCUS       DAJSKS010000193         3340 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJSKS010000193 DAJSKS010000000
VERSION     DAJSKS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.201"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..2233)
                     /locus_tag="ON396_002667"
     CDS             complement(<1..2233)
                     /locus_tag="ON396_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114706.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTSNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2905..3173)
                     /locus_tag="ON396_002668"
                     /pseudo
     CDS             complement(2905..3173)
                     /locus_tag="ON396_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          920 a          745 c          539 g         1136 t
ORIGIN      
        1 aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct ccttgaagga
       61 cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca aggatgtttt
      121 caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca tgtaccgtcg
      181 catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta cctacaggaa
      241 gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc acttgtgaat
      301 cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca tcagttttaa
      361 ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc aatcgattgt
      421 cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac gcctctacat
      481 tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc gcactgaaac
      541 gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat agaaatgaca
      601 caaattccat gccttcttcc aaaacatctt caataacaag cgtattgctt gttccagaaa
      661 acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata acatttttgt
      721 ccacactttt tcttaaagag attgccggaa gattactttc agggattctc aagcggtagt
      781 ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa aacccatcct
      841 cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat tcaataaaat
      901 acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt tccttattaa
      961 aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt gcttcacctg
     1021 gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt ccagtggaac
     1081 tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg gtaaccacta
     1141 caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta ctgttggtca
     1201 cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt tctctagagc
     1261 catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt gaaaattccg
     1321 tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt ggctcagtac
     1381 cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac tgagcggctt
     1441 catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct tcttggtaaa
     1501 atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac tcaattaagc
     1561 caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga atatcgccag
     1621 cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa gtggggcgct
     1681 cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc tgagattcct
     1741 gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag ctttcttctg
     1801 gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca cttgtgcttt
     1861 cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg cttcttgcgg
     1921 tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg taatcattga
     1981 gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat gcatttagca
     2041 ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc tcctcttgat
     2101 caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct agaaccgtta
     2161 tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac agtttatgaa
     2221 atttgttcct cactattact cccccttata attttgacta aacaacaagg tacccgataa
     2281 ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta ttttcggttc
     2341 aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt gtcttctgtt
     2401 gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc ataattatga
     2461 tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta atagttattt
     2521 tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta aatcgttgat
     2581 accaaggtca gactctagaa tttcatcagt gagtccctgt attggttaac cttcatcaat
     2641 cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga atacttggga
     2701 ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc aactaaacgc
     2761 atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta ctaataaact
     2821 gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc tttttgctct
     2881 ttatttgggc gtagttttac tttcttaact tgtatcgtct tccatcaact ccttaatcat
     2941 cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag tgacaatctg
     3001 tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta ccatatattg
     3061 caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta tagaataact
     3121 accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc ttttgttgta
     3181 gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg tattgctatt
     3241 tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca ttgattcccc
     3301 tctaaaatta ctatccataa aaaattgcat aaatgacaat
//
LOCUS       DAJSKS010000194         3304 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJSKS010000194 DAJSKS010000000
VERSION     DAJSKS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.202"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            75..464
                     /locus_tag="ON396_002669"
     CDS             75..464
                     /locus_tag="ON396_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114707.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            468..1232
                     /locus_tag="ON396_002670"
     CDS             468..1232
                     /locus_tag="ON396_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR3114708.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            1361..2308
                     /locus_tag="ON396_002671"
     CDS             1361..2308
                     /locus_tag="ON396_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR3114709.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            2438..3178
                     /locus_tag="ON396_002672"
     CDS             2438..3178
                     /locus_tag="ON396_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114710.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1090 a          543 c          701 g          970 t
ORIGIN      
        1 attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt ataaagatag
       61 gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac gtgctaagtc
      121 tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag gtagagaaca
      181 ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc tttttgatac
      241 tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct atttatccac
      301 tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt cccgagccat
      361 tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact gttggagcat
      421 ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg aatccaatta
      481 tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa gaattaagag
      541 aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag gagttagttt
      601 tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca gatgcgatga
      661 atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt tgggatagta
      721 tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa agaggtcttc
      781 tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca tgcgccttgt
      841 tggctaaaca tcctcatata gtagcgggcg tgtgtttgat gggttcccct gcacctttgg
      901 aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta ccaaaagact
      961 attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg gaaaagttag
     1021 ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct tatatacaaa
     1081 cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt gtttttcgtt
     1141 cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct tccacttttt
     1201 tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact catgacgata
     1261 gaggattttc cgttttttat tttgggctat aataaactct gttaggaaaa tttggtataa
     1321 ttgatagtaa gaatgacaac atgttagaaa ggaagagagc atgcccactt tagcagatgt
     1381 tgcaaaacgt gccaatgttt caaaaatgac ggtctctcga gtcatcaatc atccggaaca
     1441 ggtgactgac gaattgaaag agttagtttt ctcagcaatg gctgaactgg attaccgtcc
     1501 aaatattgct gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat tatttatact
     1561 ggaagaaatc gacacaactg agccctatta catgaatttg ctgatgggaa tcgctaaacg
     1621 gatcggtaaa gcgcattatt ctctccaatt agttacaaat gatgccttcg acgtcggttc
     1681 ttgtgatgga tacatcatca caggtgtcag aaaaaaagac ttcgattgga tcgaccgttt
     1741 ggaaaaacca gttgtcttgt ttggggaaaa ttctgaagga tatgattatg tagacagcga
     1801 taatgaatat ggtacagcaa aagcaacgca atatgcctta gaaagagatt atcaaacgat
     1861 tatttatatc ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa ctggttattt
     1921 atctgtgatg aaggaagttg atcttcctag taaaatcttg cggtttccaa atcattcgac
     1981 agaagtagaa agatatatga cagaaaactg ggcagatttt acagataaaa cttgttttgt
     2041 atgtagttca gatcggctag cactaggagc agtaagaggg attataaatg ctggaggaca
     2101 cttgcccgaa gaatttggtg tgattggatt tgatggggta tttttagacc aaatcagttc
     2161 acctaaattg acaacgatca agcaagatat tatacgtatg ggtgaagtat gtggagaaat
     2221 gttgttaaaa aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt tttttcctga
     2281 actagttatc agagagacaa cgaattaagt cttattacta tatatatatt tatgaaattc
     2341 atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat ctgtgctatt
     2401 attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca gacgaaaaca
     2461 tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg acaagaagcg
     2521 agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc cgtttgaaca
     2581 gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca agcgtcgtgc
     2641 aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata aaggaaaatt
     2701 accggattat accggccatt accaatcaga aatcacatcg acaggacgaa aaaaactttt
     2761 tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca agcatactac
     2821 acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc ctaaatatgt
     2881 acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc atttaatcga
     2941 atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga cgccgtatca
     3001 agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa cgttagagga
     3061 aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag aacgctcatt
     3121 acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt ttaaataagt
     3181 gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt ttccttattt
     3241 tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta agcgaatttt
     3301 ttct
//
LOCUS       DAJSKS010000195         3221 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJSKS010000195 DAJSKS010000000
VERSION     DAJSKS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.204"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            301..585
                     /locus_tag="ON396_002673"
     CDS             301..585
                     /locus_tag="ON396_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114711.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ON396_002674"
     CDS             588..1004
                     /locus_tag="ON396_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114712.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ON396_002675"
     CDS             complement(1141..1860)
                     /locus_tag="ON396_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR3114713.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2535..2630
                     /locus_tag="ON396_002676"
     CDS             2535..2630
                     /locus_tag="ON396_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCR3114714.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..3177)
                     /locus_tag="ON396_002677"
     CDS             complement(2932..3177)
                     /locus_tag="ON396_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114715.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DAJSKS010000196         3204 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJSKS010000196 DAJSKS010000000
VERSION     DAJSKS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.205"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..164
                     /locus_tag="ON396_002678"
     CDS             <1..164
                     /locus_tag="ON396_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114716.1"
                     /translation="LARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILP
                     DGNEIKIGN"
     gene            180..1022
                     /locus_tag="ON396_002679"
     CDS             180..1022
                     /locus_tag="ON396_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114717.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1085..2887
                     /locus_tag="ON396_002680"
     CDS             1085..2887
                     /locus_tag="ON396_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR3114718.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(2946..>3204)
                     /locus_tag="ON396_002681"
     CDS             complement(2946..>3204)
                     /locus_tag="ON396_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCR3114719.1"
                     /translation="GITISIPPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQN
                     FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1148 a          445 c          649 g          962 t
ORIGIN      
        1 aacttgctag acgtattgga cagactccac agaatttcaa taagaaatta caacgagaaa
       61 cggtaacctt agatgagttg aaagcgattg ctgatgtgct aggtgtcaag tttgtgcagg
      121 cttttatatt gcctgatggc aatgaaataa agataggtaa ttaggaggtg taatttgtat
      181 tgaaaatcat aacaataaat gatgttgagt atgctgtatt tgctgctaat gaaggtactt
      241 ccaaaccaca acctcatatc atagaaacga aatctggtac tattccagaa ggaaaacaac
      301 tgagtttatt aaaagaatat cttaagcaga atgatatttc cccaataaaa ggagcaacaa
      361 catattggtg cattgacaag gttcttaagc ttgattcaag taaagaaaaa actataagtg
      421 aaacaatcca taaacaaaaa tacttatctt taactgaaga aaatattgag aaacagcata
      481 agtttgtagg tgcaagctct aattatggga aagaaggtct aataatacat gatgttttga
      541 atgcatttcc tcttcataat gatttaaata ctattgcgat gaagattgcg gtaattgatg
      601 taacaaattc aacacatctt tctcaatata agagcaggtt gtctctatat gatttggcta
      661 aagtaattct ggagattcca aattttgatg atcggttggc aaaaggagat cctcagttga
      721 taaatattat cgcaaggaac attggtgcag ttaatatgtt ctcatttgca tccaagtact
      781 gtacatatca taatgtggaa gtgtgtggga gagatgatta ctccatcttt gatggtatcg
      841 ttaaaaatac tcttccacac tatatccaag gactaactac gaataagatt gatacgtggc
      901 gaagaagttt tgattacgag gcatttaacg aatgtgttgg aaaactactt gatgaaaata
      961 atatacatat tccttttaga agaagaaagt tagatcactt cttgtggtat gcgaataggt
     1021 agaatataag aaaaaggcat cgtaaaagtg attctggata aaattaatgg gggaggaaat
     1081 aatagtggga ttatttgatt ttctaaagaa aaaacaagga aaggttagta aacacgttga
     1141 ttatcaactg gcttctagtc tatttcgtac tggtaaagtt tttgctgaaa aattggaagt
     1201 ggacatatca aatgtagatg agtttcgtaa aacttttatc gcttttgatg tggaaacaac
     1261 tggcctaaat tcttcttcag atagaattgt agagattggt gcggtgctat ttaaagaagg
     1321 agttccaatt caatcattcg gaactcttgt caatccaaaa gttagaattg cagcctcagc
     1381 ttcggcaata aataatataa ctaatgaaat gctaaactct gctccaaatg agcaggaagt
     1441 ttacccccag ttggtagaat ttttaggaga tgcactagat ggaaaaatca ctatgtgtgc
     1501 acacaatgca agtttcgatt ttggattttt aagtaacaca ttttctagac ttgggtatga
     1561 tgctaaaatt aagtatgttg atactctgag tctctctaga aaacatataa aaggcattgt
     1621 aaatcataag caagagactg tagcggaata tttcggatta ataaatgaca ctgcccatag
     1681 agcggaatca gatgcagata tatgcggaaa gattttttgg ggtgttttag atattattga
     1741 ggagtcgttg gaagaacaaa aaaagcaaat agaaaagatg actccagaca taaaagaact
     1801 tgaagcttgt gcttttattc aagatattat tgagaaaaaa ggatgcgaca ccagttggat
     1861 caggtatcga aaaaacagta ataattatgt cgatgtaact tgtctatata cattcttaaa
     1921 gtttaagttt gctaagaagg gaaattacat cattattaga aaagatgcta caaatggaat
     1981 tgatttacct atagaacctt gtactgtaag tgaaggcggt acaacaaatg tgcgagtcta
     2041 ttttcgtagc ccctttgact taacgcctct ttcaaattat attttcagtg cttatagcga
     2101 ttgttataat tctatgaatg aatatatgag tgttagtaat tacactaaaa aagaagcaga
     2161 gcaaagcctt agtatgctga aatctatttc aaaatcagcg atgaaagagt tattattcga
     2221 tgcaaaaaat cgtggatatg atgagactga agttaaccta aatattgaaa aagtgatatc
     2281 tagagaagat gtaattatta acgggttgca taatcgagtt tcattaaaca agatacgaaa
     2341 tatagggaat tgggagaaag gatttgatgc aggttatcca tattgggaac gtggagaaac
     2401 tgctaggaaa aatggcaaag tagatgaagc tattgcactt tttgataaag ctcgatataa
     2461 cggctatgat gctcctgcgc tatatgattc ttatgctaaa gcttatcgaa aaattaagga
     2521 ttacgataat gaaattctca ttcttgatga aggaattatg cgtaaaacac ggcacgatgt
     2581 tggtacttta gaggctcgta gggataaggc aattaagcta ttatttgcta aacaagaggc
     2641 agagcggata gctaaggaaa aatctgattt tctaaaagcg aacaaaaaag aggatgcgat
     2701 tagtgagcct agcaagaagc gtggaagaca gattattcaa atgactgatg atggaacaat
     2761 aataaaagaa ttcgatacta tagcttctgc tgtaaaagaa gttggcgtta actcaaaaag
     2821 tattagggat gctgcaaatg gagttcaaaa acatgctgga ggatactact ggaaatacaa
     2881 agattagcag ataatctaag ataacctagg atcttcttaa tagatacccg tggtgctagt
     2941 taattttaat atcctaaaga atatctcaaa gttccttcaa aacgtcgttg tgctcgactt
     3001 aatagaacac tgtaagccag taatatgctt tcaaatctac tctctagccc tttaacagaa
     3061 cgtgagttga aattttgaca acctaatttt tctaaggaag aaaaaacagt cccaactgtt
     3121 tttctttgtt ttccaagtaa gctgtggtct agtttttctg atttatccat gtttttacgt
     3181 ggcggaatgg atatagtaat gccc
//
LOCUS       DAJSKS010000197         3076 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJSKS010000197 DAJSKS010000000
VERSION     DAJSKS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.206"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(296..910)
                     /locus_tag="ON396_002682"
     CDS             complement(296..910)
                     /locus_tag="ON396_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR3114720.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1516..1893)
                     /locus_tag="ON396_002683"
     CDS             complement(1516..1893)
                     /locus_tag="ON396_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114721.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2193..3074)
                     /gene="galU"
                     /locus_tag="ON396_002684"
     CDS             complement(2193..3074)
                     /gene="galU"
                     /locus_tag="ON396_002684"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR3114722.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          932 a          643 c          474 g         1027 t
ORIGIN      
        1 ttaagagaca agaaagaatc catatggatt ctttcttgtc tctttttcca ctacttttta
       61 tttttctgac actacatatt cttataactc atttctcata actgtgaaac aacttataaa
      121 aataacaaac tttaaacaaa acaagcttaa aatcaaagat gatccatctt aaaataacta
      181 aaacaaaaaa agtagtcgtt gttaatcctc attaataatc gttttctttt tttcaaacgc
      241 tccattttta tgtggaacat tattttttta aggaactttg tcaacatgtg gataactatt
      301 ttccacttgt gtcctggact agctgttttg ctgtaaaata caaaacttgc tgcagcaaaa
      361 agatcgttgc aagagaggct gttccgcaga tcgctggatc tagatttggc aaagtcaaga
      421 ctgcatcatg taaattggcg tggttgtcca aaaccaaatc agcaaattca aataatcgca
      481 aaccactgct atgttgcgat ttgatacttc gtgacaaatc aaatccagta acaatgatta
      541 atggatgacc attttctttg atccactgag ctagttcgat gattgccggg tttcttcctt
      601 cattcgaaag aagaagaaaa atgtcttctg atttgacaga aagctgatca ataattgcct
      661 gtcctgcacc ttctagcgtt tcatagacac ctccggctgg attttcaatc aatatacttg
      721 gtacaagatc cggggtttga tcccaaaatt caaatgctac accacttagt gtgcggctag
      781 catatatctg caatctacca ccttgattaa tttgtttcgc gatccaagtg ccagcttgaa
      841 gcatcagcgg ctcttctatc cttaatttct cttccaattg gtgataatag tcaaaataac
      901 ataaactcat ttatctctcc cgcctagcta tacttgacat gaatagaaac agctgcctaa
      961 tctaaggaag tacataaaaa aacagtagtt agacagtaac acatgtggtc tttgctgttt
     1021 caaacacaaa aaaggcattg aacgaatacg cccaatacct tgacatagca aataaaacaa
     1081 cgtgcagacc atggaaaata tctacatagg ggtagaattt tcaccctaga ttaggtacaa
     1141 taaattaggt cgatgcactg cattgactat ggcaaaatga tcggtcaggg tgtatgtgtg
     1201 tgactggtca ttccctgatt cttagcgcca actaagggtc agggagcctt tttttattca
     1261 gctgtttcta tctgaattta ttgtaagtaa tctcttatcc aagtgcaagc aagaaaatac
     1321 aaaaaaacat tggtatgatt tatatatata taaataacat aaaaaaagga atggcttatc
     1381 ccgatacaac aagcattcct ttaaaaaaga ttcttttata taagtgaaaa aagctttttt
     1441 ccaccaatta aataaatctc tcatctcaat agtttatcca ttattttatc tttttttgcg
     1501 tatacctact aaatattacc gtcttcttct cgttcttcta ctttgttcga gagaatctgt
     1561 actagaaaaa gtagttgtat gtctaccttt tctgcctatc cgagactttc ttttcatata
     1621 tgggatataa atgattagat acagtacagc aaaaaataaa gcaaatacgc aagcttccca
     1681 actacctaac gccaattcaa ccatcaaggt agcaacccca acggatgctg cataccagat
     1741 aaaaatcaga aacgtttctt tttttctgac catgcgaaac atgataaata gactaataag
     1801 caatagtggc aacacaaata tccaattgct tccttccatc agccacttga gtatatcaag
     1861 cagcgactgt gcaacagcga ctaattttcc catagtgaac acacaccttt ttttataatt
     1921 aaaaaattcg cctttttatt ctactatgaa tcttctggct tggctttcat ttctcttatg
     1981 taatcatttt cttttataca acttaaaaaa aaattaaacc attagcataa tattttctga
     2041 atctattcac gaaagtcttt ttctatcttt cgttttccac gactcaactg atctagagtc
     2101 aaaaaactaa aacgacagtt gcgcaaatga cgcacaagct gccgctttaa taacaaatag
     2161 cctttcattc tcagcaggcc acttatttcc agttaacttt ctgttcttag ctttttactc
     2221 aactggataa tatatgcact taatgaagtc ccaatttccg aatgttgaag tccatattca
     2281 atatttgtct tcatatatcc taacttatcg cctacgtcgt atctttttcc tttaaactct
     2341 ctcgcataga ccgcctctgt ctggttaaga gtctgaagtg catccgttag ctgtatctcg
     2401 tttcctgctc ctggttcttg agtttctaat atctcaaata ttttaggagt cagtagataa
     2461 cgtccgataa tggctaaatt acttggtgct tttcccattt caggtttttc tacaaaccct
     2521 tttactctgc aacgaccctt ttcgctatct gttttaggat caataattcc gtatttagat
     2581 gtttcatttt ctggtacacg catgacggca acagcggaag cttgtgtttc ttcataatcc
     2641 atcatcaatt gttgagtcaa tgggatatca tctttcatca aatcatcccc cagcatgacg
     2701 acaaacggtt catttcctat aaatgctttt gcttgaagca cggcatcccc cagtcctctt
     2761 ggatgagatt gacggatgaa gtgaagattg acttgagtcg tttcttcaac taaagcgagt
     2821 aattctgttt tttccttttc ccgcaaattg ttttccaatt cgatatttgc atcaaagtga
     2881 tcttcaatgg gtcttttttc ttttcccgtt acgatcaaga tgtcttcgat tcccgaatcc
     2941 aatgcttctt ccacaataaa ctggatcgtt ggtttatcca cgattggaag catttctttt
     3001 gccatggctt ttgttgccgg caaaaaacgt gttcctagcc cagcggcagg aatgactgct
     3061 tttttgacct tcactt
//
LOCUS       DAJSKS010000198         3068 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJSKS010000198 DAJSKS010000000
VERSION     DAJSKS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.207"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            101..1765
                     /gene="alsS"
                     /locus_tag="ON396_002685"
     CDS             101..1765
                     /gene="alsS"
                     /locus_tag="ON396_002685"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCR3114723.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            1778..2488
                     /gene="budA"
                     /locus_tag="ON396_002686"
     CDS             1778..2488
                     /gene="budA"
                     /locus_tag="ON396_002686"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCR3114724.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            2618..>3068
                     /locus_tag="ON396_002687"
     CDS             2618..>3068
                     /locus_tag="ON396_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCR3114725.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDL"
BASE COUNT         1022 a          509 c          696 g          841 t
ORIGIN      
        1 tgctatactt ttgaatgaaa caggtttaga aactgtatca attatttgtg attatttcta
       61 actaataatt aaaaataaag cataaaaagg acgatgttca atggacgaaa aaatgaaaca
      121 aggttcgaaa tctgtagtag aaagtttgat taatcatcat gttgattatg tattcggtat
      181 accaggtgca aaaatcgatg gtgtatttaa tgaattagag gatcaaggtc ctgaattaat
      241 cgtgactcgt cacgaacaaa atgctgcatt tatggcacaa gcaatcggac gtattacagg
      301 agagccagga gtagttatcg caacaagtgg tcctggtgct agtaacttag ctacaggttt
      361 ggtaacagcg acagcagaag gtgacccagt tttagcaatt gccggacaag taaaacgaag
      421 cgatctattg aagttgaccc atcaaagcat ggataatgca gcactgttcc agccaatcac
      481 aaaatacagt gcagaaatcc aagatccaga aacaatttca gaagtgattg ccaatgctta
      541 tcgtatggct aagagttcaa aaaaaggcgc gagctttatc agtatcccgc aagatgtagt
      601 ggatgcacct gtccaaggga atgtcatcaa gccattgagc gatcctaaat taggttctgc
      661 ttctgcagat gatatccgtt acctagctga acgcattaga gaagcaaaac taccggtact
      721 attagtaggt atgcgaggat caagtgaaaa agaaacgtta gccatcaggc aattagttgg
      781 aaagacagca ttgcctgttg tagagacatt ccaagcagct ggagtgatct cacgtgaatt
      841 agaagcgcat ttctttggtc gtgtgggttt atttagaaac caaccaggag atatgctttt
      901 gaaacgaagt gatctagtta ttgctattgg ctatgatcca atcgaatatg aagcaagaaa
      961 ctggaatgcg gaaaaagatg cgcggatcat tgtgatcgat gaagcgccag ctgaaatcga
     1021 tccttttatg cagcctgaaa gagaattgat cggtgatatc tcagccactc ttgatttatt
     1081 gacaggttca ttagagcctc aacaagtgtc agaagatgca aaagaatatt tagcatctct
     1141 tcaagcagaa ttaacggagc gggacattgt tcaatcaaaa ggagaagcag gtatcttgca
     1201 tcctttagaa gttattaaca cgcttcagtc aaaagtaaca gatgatatga ctgttaccgt
     1261 agatgtaggt agtcattata tttggatggc acgccacttt agaagttatg aacctcgtca
     1321 tttgttattc agtaatggta tgcaaacgct tggtgttgca ttgccatggg cgatttctgc
     1381 tgcattggtg cgtccaaata cacaaatcgt atcagtttcc ggcgatgggg gatttttatt
     1441 ctctgcccag gatttagaaa cggcggtacg taaaaaattg aatatcgttc atttgatttg
     1501 gaatgatgga cactacaata tggtagagtt ccaagaaaaa atgaaatatc agcgggcgtc
     1561 aggtgtagac tttggtccag ttgattttgt gaaatatgca gaagcttttg gcgctaaagg
     1621 aatccgagct acaagtgtag aggaattaga aaaagcttta gaagaaggat ttgcgacaga
     1681 aggacccgta attattgata ttccgattga ttaccgtgat aatgagaaat tgggagaaac
     1741 aatcttacca gatcaattct attaaggaag tgtcgacatg actgaaaaaa tcttatacca
     1801 gcacgggaca ttaggagctt tgatggctgg cttgatggat ggaacagaaa cgattgctca
     1861 tatattagaa aaaggaactc tgggattggg aacattgcat gggcttgatg gtgaagtcat
     1921 ctttttagat ggcgtagctt atcaaggaag atcagatggc tcagttgccc aattagatgg
     1981 aagtgaactt accccctatg cagcaattac agattttacg ccagatacta gtttttctgt
     2041 ctcaaaaact gcagatggag aacaattgaa aaaagacata ttagtaagag aagccgggga
     2101 aaacctattt ttggcagtga agataactgg attattcaaa aatatgcaca tccgtatcat
     2161 gccgaagcaa gaaaaacctt acagaagact agcaaaaatt tcagagtctc aaccagaatt
     2221 ccaacaaagc aatgtccaag gaacattagt cgggttcttt acgcctgaat tattccaagg
     2281 agttgctgca gcgggatttc acttgcattt tatcgatgat acacatacat ttggcggaca
     2341 tgtaatggat tttgaagtgg ctgaaggaaa agttgagatt agccgcatct cgtcccttgt
     2401 acagcatttt ccagttgatg atccgacttt tgttcacgaa acgattgatt atgctgattt
     2461 agcagcagaa atccaacaag cagaatagaa aaagacagcg agatgcgttc aaggtgatta
     2521 acctgacgta tctcgttttt ttgaggagga aacctagcgt ttacaaaaaa actgagctat
     2581 aatcttctcc atagatcaaa aaggagcggt ttttatcatg tggacagaaa aacagaaaaa
     2641 agatgcatac gaaattttat tattacagca acaaggatta ttagaagcag aagataactg
     2701 gttagcaaat ttagcaaatt catctgcatt gttgaatgag actttgcctg agactgtctt
     2761 tgcaggatat tatttattcg atggtaatga attgatttta ggaccgttcc aagggcgagt
     2821 ttcttgcaca cgtatcaaga tgggaaaagg agtttgtgga gaatcagctg aaaaacaaga
     2881 aacattgatc gttgatgatg tcaaaaccca tgcaaattac atttcctgcg attcagccgc
     2941 acaatcagaa atcgtagtgc caatggtaaa agaaggtaat ttaatcggtg ttttagatat
     3001 cgattgcgga gtaacgaaag ggtatgatga aatcgatcaa gcatttctag aaaaatatgt
     3061 agatcttt
//
LOCUS       DAJSKS010000199         2994 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJSKS010000199 DAJSKS010000000
VERSION     DAJSKS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.208"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            154..948
                     /locus_tag="ON396_002688"
     CDS             154..948
                     /locus_tag="ON396_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR3114726.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            938..2980
                     /locus_tag="ON396_002689"
     CDS             938..2980
                     /locus_tag="ON396_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3114727.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT          959 a          482 c          606 g          947 t
ORIGIN      
        1 aggtgaaaac ttgtagctgg tggatttgtt ttttgcatat ttaattttgc tgaataaaat
       61 aattgaataa aattttttat tcagtccaag taaaagtgac aaaaacataa gagcaaatta
      121 ttcgtagata tgctactatg caaataaata gaaatgaaaa atgaaagagg aggagaagaa
      181 ttggaaaaga tgatcgaagt gaaacatcta agtaaaagct acggcagcaa actttctccc
      241 gtcaaagcct tagataatat ctcattcaca gtagaagaag gcgagtttgt tgggattatg
      301 ggcccaagtg gtgcaggaaa gacaacgctg atgaatatct tgtccacgat caatttgcct
      361 acgatgggca gtgtctcgat ccaaggaaaa gaaatcacaa aaatgaaaaa cagtgagctc
      421 agtgactttc gtcgaaaaaa actaggattc atttttcaag agttcaattt gatcgatacc
      481 ctaaatgcaa aagacaatat cctattgccc ctagcagttg aacggatgac taaagaggaa
      541 atggaaaaga gagtaagaca tgtagctcaa ttgttgaata tagaagaact gctcaaacgg
      601 tatcccgatg aactttctgt cggacagaga caacgaattg cagctgctag agcacttgtt
      661 gttcagccac aagtgatttt tgcagatgaa ccaactggct ctttggactc gaagtcggct
      721 acagaattgt tgaactactt gaagaccatg aatcaaaaag aaaaagcaac gatactgtta
      781 gtcacccatg atccatatac agcaagctac tgcgatagaa ttttatttat taaagatggt
      841 gtgatttttt ctgaagttgt cagaagaggg acaagaagag agttctttga gaaagtaata
      901 gacatgcagg cgacgattgg cggaggagga aaaatgaatg ctgtataaac tagccgttaa
      961 aagttttttt cggcagtcac gaggctatct tgtttatttt ttcagtttga ctttatccgt
     1021 aatgatctat tattccttca gcgcaatgac ctacgatcaa tctttagtca gaagagcaag
     1081 tcaagatact tcgatagatg gtggattgag ttttggtgga tttatcatta ctgttgtttt
     1141 gctttttttc gtattcagtg ccaaccgatt tttcttgaat agaaggcaaa aggaaatagg
     1201 catctatcag ctttttggta tgaataaact gcagatatca ggtatatatg tgttagaaat
     1261 catgataatt ggattgttcg cctgtatttc tgggatatta ctcggtatta ttttttccaa
     1321 actcttttca atgattttgg ttcgaatgat ggatatggat cttactagtc cattttttat
     1381 ctccattcca tcagtagtag atactttatt tgcttttttc atcatccttt tagcagtatc
     1441 tgtatattca atatggaaaa tatggcgcta tccaatgatt cgaagttttg gagaaaaaga
     1501 acaagcagat agttcaacaa tgcgcttcag gacaaggcac cgattactcg cagtcatcgg
     1561 gttactactt ttaacgagcg gatattttgg agctagtcat tttagggaaa tcacttcatg
     1621 gatgatcagt tatcattatc ttgatacatt agttttcttt tttccttttt taattttttt
     1681 cgtttgtgtg gtcggtactt atttgttttt ctgttataca tttcggttat ttttgatcat
     1741 attgagtaaa tggaagattt attactatgg tatcaatttt ttgataatgg gtaacacgca
     1801 agttcatttg ctcaaaagtt ggcgtacaaa ttctctgatt acagtcattc tcggaatttc
     1861 tttaatgaca attggcggca cggctagtat ctctactatt ttatcctacc aggagaaagt
     1921 ttcctccccg atggactatc agtttgatgt tgagacagag aaaaaaatca agccgattct
     1981 agcagaggaa gggcagaaga taactaaaga aatcaccttg cattataaag tcattgggag
     2041 tatctatagt caaaatctcg aagaacctat gtttcaaact gctaatctga tcacggaaac
     2101 acaatatcaa gccttcagaa aagttaatcc agatttgccg aacatttctt tgaaatcacc
     2161 tggtcatact gttttgcttg atcaaatgca aactcttttt cggaatattt cattatatgg
     2221 atctagtatt ggactgccag ataatcaaga attgaagatt caacaaatgc ttccaagtgt
     2281 attaggtgac gaaacaatgg tatatctaag tcctgtactg attgtagatg ataccgtttt
     2341 tgaaaaagca aaaggtgtgg attatcaagt agtcaatgta gatgtatcag gtggaaataa
     2401 tgaaaaaata gatgaacgac tgagcaatga cctgtcgata aagtggctca atgctgttta
     2461 ttattcttat gatatcgtaa atggaagtct acaaggagaa attagcacaa agaaacttcc
     2521 ggatgaccaa atgaaggaag cacaatttag ccaagaaagc tctcgtttga atttctcttc
     2581 cagctattcg aaattgcgtg cagttaatcg gcgaatcggt atcaatattt ttgtcacact
     2641 atttgtcggg atgatcgtga ttattgctac tggtagtatt ttgacagtcc ggcaattagc
     2701 agaagcagag gaagaaaaag aaaattatca cctattgact aagctgggga ttccacaaag
     2761 aaggatcaga cggatgatct tcgagcaaaa cgcacttacc ttctttccac ccatgatatt
     2821 agggatcact cacgcagtat ttgcaatcaa tgtcttcact cagtatataa aaactgccga
     2881 ttactggttg gcttatcttg tgagtggctt attgatcctt atctatcttt tgctttatgt
     2941 cattacgagt cagttatatt gtcggattat cgaggaatga tatacggaat aaga
//
LOCUS       DAJSKS010000200         2968 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJSKS010000200 DAJSKS010000000
VERSION     DAJSKS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.209"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(37..1257)
                     /locus_tag="ON396_002690"
     CDS             complement(37..1257)
                     /locus_tag="ON396_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HCR3114728.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ON396_002691"
     CDS             complement(1277..1888)
                     /locus_tag="ON396_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR3114729.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ON396_002692"
     CDS             complement(1931..2863)
                     /locus_tag="ON396_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCR3114730.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
BASE COUNT          812 a          622 c          515 g         1019 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaac
//
LOCUS       DAJSKS010000201         2965 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJSKS010000201 DAJSKS010000000
VERSION     DAJSKS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.210"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(230..592)
                     /locus_tag="ON396_002693"
     CDS             complement(230..592)
                     /locus_tag="ON396_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114731.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(666..1046)
                     /locus_tag="ON396_002694"
     CDS             complement(666..1046)
                     /locus_tag="ON396_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCR3114732.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1110..2303
                     /locus_tag="ON396_002695"
     CDS             1110..2303
                     /locus_tag="ON396_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR3114733.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2379..2963)
                     /locus_tag="ON396_002696"
     CDS             complement(2379..2963)
                     /locus_tag="ON396_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3114734.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          922 a          569 c          492 g          982 t
ORIGIN      
        1 atttatcatg tatttcttcc ttggtcaact ataaattaag cattactttg tgaaatgatt
       61 tttcgatcaa ttattattat tcgttataca accgttttca acatattttg aaaataaaat
      121 gcactaaaaa actatcttta gcagaacatt tcattgtatt ttttggacca aacaataaaa
      181 aaagaagttt gaaaaaaatt caaacttctt tgttcgctta atctgtattt tagaagaaag
      241 gcactccctc tacttcgtaa gttccaagaa cagagcgcat ttgacctggt tcttcccaaa
      301 tatatccttc gcctaattct tctctttgat tcttttgttc agtttcaaag acaaacaacg
      361 gaacattttc ctctttaata tgtgtatttg tcaattccat caaactaatc ttgctaacat
      421 cgatatgaac tatttctttt aagaaattaa gtatattcgc taaaccagtc tttccttcag
      481 aaagttccgc taacgcaaat tccaacttgt catcaattga atggaccaaa aattttttag
      541 taccatcctc caaatgcaac ataattgtac cagcaactct tttgcttgcc aaaatttcca
      601 cctcgatcaa cttgttctat ggcttacata aaaattctag cacattttca gaaaaaatac
      661 actttttatt tcttattttc tttccgttct agttcagata atgcttcttc tatccaaata
      721 ggtaactgct ccgcaatagt ggtaaatccg atcttacgat ttttattcgt gaaatacctt
      781 tttctgcgat atggaacagc tggatttgtt ttttctaacg ttcttaaaga taaactgatt
      841 tttttggaat attcatcaat gtcgatgatc tgaactgtta ctggctgtcc gacttttaac
      901 aatgaatgga tatttttcgt ataaccggat tgtacttctg aaacatgaat aagtccttgc
      961 gtctcgttat caagagaaac gaatgcgcca taaggctgta tccctgtcac ttttccttca
     1021 attatttgtc caattttata agtcatctac ttccctctct tcgcatttat cattatactg
     1081 taaatagctc aaaaaaagaa gcgacgaaaa tgactggagg tcttaagatg atcgaatttg
     1141 ataaacagat ttcaagaaaa gatacagaaa gtgtaaaatg ggacgcaatt gctgagacat
     1201 atcaaatgga tgacttactt cctttgtggg tagcagatat ggacttcttg tctcccggtg
     1261 gggtagctaa agcgtttagt gaatacatca aacatggaat ctttggttat gccactctgt
     1321 ctgataaact atatcaggct atcattcatt gggaacgcca acaccatgct gtggagttga
     1381 cgaaagaaaa tatcgttttt acaagtggtg tgttaaccag tttagctgca gcagtacaaa
     1441 cttttacaaa accaggcgat tctattttga ttcatgaccc agtttatcct ccattttcct
     1501 ctattataga aacaaatcaa cgtcacatcg ttcgaagctc tttgctagaa aaaaataatc
     1561 atttcatcat ggacttgtct gacatggaga aaaaaatcga agaaaacaat gtaaaagcaa
     1621 tgattttatg caatcctcac aatcctggag ggcgtgtttg gagcaaagaa gaattgaaac
     1681 agttaagcga attgtgttta aaacatcaag tgattctgtt tagtgacgag atccatcaag
     1741 atttaacttt attcgatcat acatttactt ccatgctaac gattgatcct aatttagatg
     1801 atctactagt tgcttttact tccgcaacca agactttcaa tctagctgca atcaaaaatt
     1861 caatggtatt tatcaaaaat ccagagatga aagctgattt tgaaaaacat ttagtgatga
     1921 accaacagca cgaaatcaac acttttggac tgattggaac ccaagcggct tatgagacgg
     1981 gtggagaatg gttaagccaa ctgattcctt atctagaaaa aaatgttcaa acagttaatc
     2041 agttttttga acagtatctt ccgaaagtga gaataatgaa ccctgaagga acttacttaa
     2101 tgtggttaga ttttaccgcc tatatttcag acgaccaaga attggagaac actcttgttc
     2161 ggaaaggaaa agtcgtatta aatcctggca tcacgtttgg tccttccggt catgggcata
     2221 tgcgcttgaa tattgcttgt ccagaagaaa ctttactgga aggacttaat cgaatcaagt
     2281 atgctttaga gacaatcgat taaatattta ttcaaaataa aaggacgaga ccttactagg
     2341 tttcgtcctt tttcataatt actccgtacc ataccaaact attctgaaat ttcaattgtt
     2401 tcaatcacta catcatgtac aggtcggtct tgtggaccac gttgtacatt tgcgatttca
     2461 tctactgtgt ccatcccttc tagtacatgt ccaaagactg tatgtcggaa atctagccaa
     2521 ggagttccgc cacctttata cgcttcaatg atttctgatg ggaatccagc accttctagt
     2581 tgtccaagca tattagctgg cacatttgtg ttatttacaa taaagaattg gcttccgttt
     2641 gtgtttggac ctgaatttgc catagataaa gctccgcgaa ggttgaacag ttcacgactg
     2701 aattcatctt caaaactttc gccataaata ctttcgccac ccattcctgt tccagttgga
     2761 tcgccgcctt ggatcatgaa atcaggaata acgcggtgaa aaatcacgcc atcataataa
     2821 ccttttttgg aaagttcaat gaaattttga accgtcttcg gtgctagttc agggaataat
     2881 tgaacagtaa tgtcaccacg gtttgttttg ataacagctt ttggtccttt ttcacttttt
     2941 aaatctagtt gtgggaatgc catat
//
LOCUS       DAJSKS010000202         2931 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJSKS010000202 DAJSKS010000000
VERSION     DAJSKS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.211"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            87..983
                     /locus_tag="ON396_002697"
     CDS             87..983
                     /locus_tag="ON396_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3114735.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1081..1290
                     /locus_tag="ON396_002698"
     CDS             1081..1290
                     /locus_tag="ON396_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HCR3114736.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1308..1580
                     /locus_tag="ON396_002699"
     CDS             1308..1580
                     /locus_tag="ON396_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR3114737.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            1582..2445
                     /locus_tag="ON396_002700"
     CDS             1582..2445
                     /locus_tag="ON396_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HCR3114738.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1162 a          464 c          510 g          795 t
ORIGIN      
        1 aagagggctt ttatatatta atcacaaatc acttatcaca aatcacaagt gattaatcac
       61 aaatcacttg tgatttgtga ttcttaatga tacaatatta ctatacaaaa aaagaatggg
      121 gcgtagttat ggagaaggaa aaactaaaaa tacttgaaga attaagacgt attttaaaca
      181 ataaaaatga agcaattatt atcttgaata attactttaa aggtggtgtt ggaaagtcca
      241 aattatcgac tatgtttgct tacttgacag acaaattgaa tttaaaagtt ttaatgatcg
      301 ataaggactt acaggcaaca ttgacaaaag acttagcaaa aacttttgag gtagaattgc
      361 cacgtgtcaa tttttatgaa ggcttgaaaa atggaaactt ggcttcttct attgttcatt
      421 tgactgataa tttagacttg atccctggca cgtttgattt gatgttactg ccaaaattaa
      481 ctcgctcatg gacttttgaa aatgaaagta gattgcttgc tactctttta gcacctttaa
      541 aaagtgacta tgatctcatt attattgata ctgtaccaac gccaagcgtt tatacaaata
      601 atgcaatcgt ggcgagtgat tacgttatga tccctttaca agcagaagaa gaaagtacaa
      661 acaacattca aaactatatt tcctatttga ttgatttaca agaacaattt aaccctggac
      721 tagatatgat cggttttgtt ccttatttag ttgatacgga cagcgcaacg ataaaatcaa
      781 acctggaaga actgtacaag caacataaag aagataactt ggttttccaa aatattatca
      841 agcgaagtaa taaagtaagt acctggtcta aaaatggaat tacagaacac aaaggctatg
      901 acaaaaaagt tttatccatg tatgagaacg tattttttga aatgcttgag cgaattattc
      961 aattagaaaa cgaaaaagaa tagaatcaca aatcacaagt gattaatcac aaatcacttg
     1021 tgatttgtga ttgttgatga taaaataaga ataagaagaa atagaaagaa gtgagtgatt
     1081 gtgggaaatt taggcgcaca aaaagcaaaa cgaaatgata caccaatcag tgcaaaaaaa
     1141 gatataatgg gagataagac ggttcgtgtt cgtgctgact tacaccatat tataaaaatt
     1201 gaaacagcaa aaaatggcgg aaacgtaaaa gaagttatgg atcaagcctt agaagaatat
     1261 atacggaaat atttacctga taaactttaa aaaggagtga aaatgaaatg gcagttacgt
     1321 atgaaaaaac atttgaaata gagatcatta acgaattatc ggcaagcgtt tataatcgag
     1381 tattaaacta tgttttgaac catgaactag atactaaaaa tactcgttta ctagaagtga
     1441 atcttttaaa tcaattagaa gtggcacaag aagttgattt atttcaacaa ccatttgaag
     1501 aattacaagc tattcatgag tattggcggt caatgaatca atattcaaaa caaattttga
     1561 caaaagagaa agtggcttaa catggcgaat attactgact tcaccgaaaa gcaatttgaa
     1621 gatcgtttag aaaagaatgt tgaacgacta actaaaaata gactagcggt tgaatcgcca
     1681 accgcttttt tacttggtgg gcaaccagga tcaggtaaaa ctagtttgcg atcggcaatt
     1741 tccgaagaga cacaaggaaa tgttgttatc attgataatg atacgttcaa acaacagcac
     1801 cctaattttg atgaactagt gaaactttat gaaaaagacg tagtaaaata cgttacccct
     1861 tattctaatc gcatgacaga agcgatcata agccgtttga gagataaagg gtataattta
     1921 gtgattgaag gtacaggacg aacaacagac gttcctattc aaaccgcaac aatgcttcaa
     1981 gccaaagatt atgaaacaaa aatgtatgtc atggcagtac ctaaaatcaa ctcatattta
     2041 ggaacaattg aacggtatga aactatgtat gcagatgatc caatgacagc cagggcaaca
     2101 ccaaaacaag cgcatgatat tgttgtcaaa aacttaccga ccaatttaga aacccttcat
     2161 aaaacgggct tatttagcga tataaggctt tacaatagag aaggagtgaa actctattca
     2221 agcttagaaa cgccttccat tagtccgaaa gagaccttag aaagagaatt aaatcgtaaa
     2281 gtatcaggga aagaaattca accgactttg gagagaatag agcaaaaaat ggttcaaaat
     2341 caacaccaag aaacccctga atttaaagca attcaacaaa aaatggaaag cttgcagcca
     2401 cctacaccac caatacccaa aacacctaaa cttccaggga tttaaacctt aaaaaagtaa
     2461 aagagtagtt accaaaaaac ggtaactact ctttttttat aaaaacattt cctcattttt
     2521 taaaagctga tcgagaaata aagcacacaa ctgtattctt tcttttttca gttggtacat
     2581 tttgcagtct gtaacgtgcc atttactgtt tgttacctta acatatccag tcgaaaacac
     2641 aagggtatat aaacgagcaa gctcgccctt gtgttttctt tgtgtctatg ttgcgttctc
     2701 gtcacagcaa acgacacgaa cacactggcg aaaatgaacc aacaaaacga aaaaagaaag
     2761 gagcgtacac tgtaatttat ttctagcttt taaaacatga ggaaaacagc ttcacttcct
     2821 caaacgccta acaaaaatgg ttgtcaaaaa actaaaaatc aaaaaaagaa aagaggaaaa
     2881 gaaaatggaa aaaacaaaaa aattacaatt agaagatttt acagaaaatg g
//
LOCUS       DAJSKS010000203         2913 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJSKS010000203 DAJSKS010000000
VERSION     DAJSKS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.212"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            349..1674
                     /locus_tag="ON396_002701"
     CDS             349..1674
                     /locus_tag="ON396_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR3114739.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1667..2017
                     /locus_tag="ON396_002702"
     CDS             1667..2017
                     /locus_tag="ON396_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114740.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1999..2184
                     /locus_tag="ON396_002703"
     CDS             1999..2184
                     /locus_tag="ON396_002703"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114741.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
BASE COUNT         1029 a          457 c          547 g          880 t
ORIGIN      
        1 ttattacaaa aactattaag aaaacttcta catacaacta ttacaaaact attaagaaac
       61 tgaatactcc tattttttgt ttttaaggaa agttttttct tttttgaatt tcttattttt
      121 ttctaatata actaagcaaa actggataat acgacactta ttttcaaatt ttcacaagac
      181 taatagtaaa tatccattta ataaagtgat acaatgtgtt gattttacaa atcaacagaa
      241 gaagtaggga aataacttac ctatgcttct ttttttgtac atttaatcga ttcttcttta
      301 caaaaacgaa cgtatgtttg tataatgtgg aagaatggag tgttatttat gatggattat
      361 tcaaaagagc cagtaaatga ttactttttg atcgatatga aaagctttta cgcaagtgtc
      421 gaatgtatag aaaggaattt agatccatta acgactgaat tagtcgtcat gagtcgagcg
      481 gataatacag gatcagggtt gattttagct tcttcaccag ctgcaaagaa gaagtacggt
      541 atttctaatg tcagtcgacc acgagatcta ccgcaacctt ttcctaaaac gttacatgtc
      601 gttccaccac gcatgaatct ctatattaag cgaaatatgc aggtaaacaa tattttcaga
      661 aggtatgtag cagacgaaga tttattgatt tactcgatcg atgaatcgat cttaaaagtg
      721 accagatcaa tgaatctttt tacgactgaa ggaacaagaa gtcaacggag aaagaagctg
      781 gctcaaatga tccaggagaa tattaaagaa gagctaggat tgcttgctac agtaggtgtc
      841 ggagataatc ccttattagc taaacttgct ttagacaatg aagcaaaaca taatgatgga
      901 ttccttgctg aatggacgta tcaaaatgtt cctgataaag tatggaatat tcctgaaatg
      961 acagaccttt ggggtattgg atctcgaatg aaaaaaagac tcaaccaaat tggtatcatg
     1021 agtattaaag acttagctaa ttggaatcca tacactatta agagccattt aggtgtgatt
     1081 ggcttacagc tgtattttca tgcgaacggg atcgatcgaa cagatatagc gatcccacca
     1141 gaaccgacaa aggaaaaatc atatggtaat tcacaagtgt taccacgaga ttataccagg
     1201 agaaatgaaa tcgaactggt ggtaaaagaa atggcggaac aagtagctat tcgtatcagg
     1261 caacataact gtaaaacagg ttgtgtccat ttatacattg gcacgtcgat cttagaaacg
     1321 agaccaggtt tttcacacca gatgaagatc cctataacag ataatacaaa ggaattgcag
     1381 aattactgtc ttttcttgtt tgataagtat tatgaaggac aagaagtaag gcacgttgga
     1441 ataacctata gcaagcttgt gtacactgat tcgcttcagc tggatttatt tagtgatcca
     1501 caaaagcaaa ttgacgaaga aaatttagat aaaattattg acaagattcg gcaaaaatat
     1561 ggttttacgt cgattgtaca tgcttcttct atgttagaag gcgctcgtag tattactaga
     1621 agtacattgg ttggtggcca tgcaggcggc gcaggagggc ttaaaaatga ttgattataa
     1681 tgatcgtgga cgtttaaagt gggcaggttt ttatttatct gatcatactg aaaaaattga
     1741 tgccgacaat gaacaacgag ccaatcaaaa tttggcaaaa gaacaaatga cgactgaaga
     1801 ggttgcggaa gtattgaacg ctgcagtcat aaaaaataga tcagtatgta ttcaaaaaga
     1861 agaacgaaac gctgaaggat actatccaga agatattgtt agcaagatct taggtagtga
     1921 tgagctgggg atctacgttg gaaaaaacaa agttcactac gacgaaatac gtcatatcga
     1981 attttaccag gataataaat ggggtcgatt agaatgaaca ttatccagca atatgagcta
     2041 aagtatataa cctttgatca gctatcagaa gagatttggg gttatggcca gcgtttgata
     2101 aatgaggttg gtgttgaacg cttctctttc tatgtagaag ctgcagcagg ataccataaa
     2161 tacgaatact atattggcta ttaaagtgca ataaaattat atttctttta tgttttacat
     2221 tttgtatcaa ttctgataaa attacctata ggtaattaaa aaatataaaa aaactagtta
     2281 caaccttagg acgttgtaac tagcttgaag catactagag ctaactaaaa tagcactaga
     2341 aagcaatgtt aaacccgtcc aacaaacttg atttaacatt gagaccagct tattcagcta
     2401 aatatagttg aataagcttc ttttgtttta aaaaaacaaa aggtgtacta cgaataacgc
     2461 aagtaaaaga aatactatta caaccagtag gctacaagta agtattacta aacattgcgt
     2521 agttagaacc tttgctctat tgtctaaagc cacaaaatta gagatcagct ttatgatgct
     2581 aagagcgagc tgcatccatg cggacatagg ttcttttgac actattaatt ctccttttta
     2641 ggataaggga aattgctagt agttttttca ttagcaccat cccccttcat ttgattgagt
     2701 ctagtgctgc tgtgatagtt tgctgtttaa tatgcttaca ttcttattat aacataaatg
     2761 aaacaaacac atggttagag gtcgtatcaa tcaaaatagt gtctcacaaa cgatttatta
     2821 cttactcctg ctgactaacg gactgttctt cagtaactgt aactatatgt ttgttaaaac
     2881 caccattacc tgtagataaa aaccaatata ttt
//
LOCUS       DAJSKS010000204         2907 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJSKS010000204 DAJSKS010000000
VERSION     DAJSKS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.213"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            123..938
                     /locus_tag="ON396_002704"
     CDS             123..938
                     /locus_tag="ON396_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114742.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1064..1321
                     /locus_tag="ON396_002705"
     CDS             1064..1321
                     /locus_tag="ON396_002705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114743.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1573..2145)
                     /locus_tag="ON396_002706"
     CDS             complement(1573..2145)
                     /locus_tag="ON396_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3114744.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2161..2766)
                     /locus_tag="ON396_002707"
     CDS             complement(2161..2766)
                     /locus_tag="ON396_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HCR3114745.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          950 a          528 c          451 g          978 t
ORIGIN      
        1 ttaaataaaa aataattact atctagtacg aaattaatta cataggataa tatattaagg
       61 tgctggacta caagtaaaaa atactgtatg atcatgacag attcaagaaa ggagcaataa
      121 gattggttaa gatagcagga ctttctaaag gtggaaaaac cgtttcacaa ctttataaac
      181 aaaactacag agactggaga cagttcagaa aaaacattaa ggattcttac tttattctac
      241 caacagaact tgaaaaatat ttaccgaata acaaagctat taatttatat ctgtattact
      301 gttttaaggc aaagaatgaa ggcggggatt cctggcattc ggtcaatacc atagcagagt
      361 ctctaggagt gacaactaaa actataaaca cctggaataa aaccttagtt gatttaggta
      421 tgattgcacg tatagacgat aaccatctca gtaaatcgac aattttactt cctctctcta
      481 gttactataa attagccctt gatagttctt taaaggatgt taccgagagc actgtccatg
      541 aaattgatgg caacttggtt gcagtttatc atttgtttga atggagaaaa aatgaggctg
      601 atgaaaatta taacgttcct tataacacac tctgttgtgt ttttaaaaga aaaacacaaa
      661 aagatactat ctataaattt atcttggtcc aagatgagaa aataagtaat ttcgaattaa
      721 gcactccttc tagtaagatt tatgacgatg catatagatt tgaatgtcaa gatctagaag
      781 ccctcctttc cgcaaataat ctaaacaata tagaacatga aaactttgtt gtaaacacta
      841 gatttaattt agtttctgaa aaggactcag atttgttaga tttgctgata gagcttacta
      901 aaaatctaga taagaaagaa aacattactg tactataact agcatcggtc catcattaag
      961 tttggactat tagaacactt gctattacag aaaaaggtat gctagccacg cttactattt
     1021 attggtatca aacaagtatc ttaatttgaa aggaagatga tttatggcta atttatatat
     1081 agagaacaaa ggagacttga atgccattat tactttaatc gtagttatgg taaaatcaat
     1141 aggtaatatc acaataggaa tgctgtttat tatattagca gcagtgctga tttttatgat
     1201 agttatttgg ttcttaaatg aaagatctaa agacaatcaa caccgtaggg atatggagat
     1261 aaaatcaata gagtcaatag attatttatc gataataaca ctaaaaaagc cacgttccta
     1321 aacgtgactt acgctgaacg atatgtgttg tttggtagac gaacaaatcg ttcattataa
     1381 atatatgatg gtatcctaat atagtaagcg tggctagcat acctttaatt actttacctt
     1441 ttacagttac catacacatt ttttgagcag aaatcaagta aataaaaaaa tattatattt
     1501 cacaacatgc taatagactc atatctagaa cgcattaagt atttacctgt ctggttcgct
     1561 gagacttctt ttttaacttt cttcaatttt cttctttatt ctaaaaagcg tactgcgact
     1621 gaaaccggtt atagcttcaa cttccttata cgatttacct gaagtaagca gttcatatgc
     1681 gtgcctcttc ttcggtgtga ttgttgcttt cggacgtcct tctctgaaat taggattatt
     1741 ctttcttgca aacatttttc cttcttgggt acgttccaca atcatatcac gttccatttc
     1801 tgctacactc aacaacgtcc gtgtcaccat tcgccccatg gacgtatttt caatcgttcc
     1861 tagattcaaa accttgatcg ttacgttatc atcgagtaaa ggttcaatga tctctaatgc
     1921 ttctctcgtg ttacgagcaa atcgatcaag cttcgtaaca actatttcat ctccaggctt
     1981 cagaccagcc aacagctgat tgaattttgg ccgatcccgt tttgtacccg taaatttttc
     2041 tgaaaaaata ttcttcttct ctacgccgtt ctcaagcaac tgttgaattt gttcttttgt
     2101 actttgagaa gcggttgaga cacgtgcata tccatatttc gtcatttggt ccacctactt
     2161 ttacaaatca tctatcgtat actcagaata cccttcatag aaatagctga catcgattcc
     2221 tttcatatat aactcgcgat cgtcaatttt atcagtcaga gcattggaaa ttagatagcg
     2281 tatttccaaa tcgtttatgg gacttcgctc cattgcagaa agatagtctg ctttatcgat
     2341 taagttccaa tctacaactt tttgcaattc ttcctttaaa atgagatcca gccaaattct
     2401 agtacttcgg ccatttcctt ctcgaaatgg gtgagcgata ttcatctcca catatttctt
     2461 gataatctct tcaaaagaag tttgtggcat ctgatcaata tgctgtaacg aatgttctaa
     2521 atacataact ggtgcaaatc gaaaatttcc ttttgcaata ttgaccgatc ggatttttcc
     2581 ggcaaaatcg taaatatccg aaaatagaaa ttcatgaatt tcagataaac ctttaaatgt
     2641 accaacttcg atttcattta tttttccaca atcgtacaat tctttcgctt tttgtttact
     2701 gagtttttct tcaaccttag ccaactcaac ttgattggtt aatcccaatt tattctctaa
     2761 aatcatgcga aatctcctta actttatgac ataagtttat gaaacactct caaccccatt
     2821 atatcgcgat tttgtttatg tgtcaaaaac tattaagttt ctgacccccg atagattttt
     2881 caagtgtctc tattttgcac agtctag
//
LOCUS       DAJSKS010000205         2887 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJSKS010000205 DAJSKS010000000
VERSION     DAJSKS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.214"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(59..1369)
                     /locus_tag="ON396_002708"
     CDS             complement(59..1369)
                     /locus_tag="ON396_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCR3114746.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1381..2799)
                     /locus_tag="ON396_002709"
     CDS             complement(1381..2799)
                     /locus_tag="ON396_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3114747.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          900 a          602 c          450 g          935 t
ORIGIN      
        1 cattaaaaaa aataaaggtc aagctaaaat attatttaag cttgacctta ttataatttc
       61 aacgattgtc aaaagtattg tttgcagctg tgttgccagt ttctatagat aaatcacagc
      121 ttttgaaaca taagttttgt actgcttcga ttcgcccgaa ttcttttgca gttatttggc
      181 aatgagaaaa gcagacatct tctataggct tttcaggaaa tgctactaaa tcaaaagctc
      241 gggaaggaag tgagtaacct ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt
      301 tgactttcgt catttgcttt gattcaggta tttcttcagc cactgcttgc caactttctg
      361 gcatttgagc aaggtcattg ctattttttc tattgtattc attatgccag tccattatcc
      421 aagaaaaagg aaattgtaca tcaatcatat tcagattttc taccctgata ttttcgatca
      481 ctccccctct ttcttttgaa gatttcattc gaataccaca gtctgactga aaaaaattga
      541 tatcgtgaat atatacgtca ctaactccag cactaacttc gctaccaata gttacaccgt
      601 atcctgaacg gattttacat ctagcaacct cgattcttga tgattttcga ttgactcttg
      661 caccattgcc atctctgcca gacttaatag caatacaatc atcaccacag gccaaatcac
      721 attcatagac acgcacattt gttgaagaat caatatcaat cccatctgta cttggtccat
      781 cattgttgcg gatgattact ttttctacta atacatcgtt agaataagtg atttgcaagt
      841 tccaaaaacc tgatttctga aaaatcaaat cacgaacaac aacatgttct gcatgataca
      901 gtaaacaagc gcgtggacgt ttaatagcat aatccgcaat ccaacgaagt cccttttgat
      961 cgtattcagc tcttgtcccg cttttttgat cttttcccca atacagctcc caccaatggt
     1021 ctccttgacc gtcgattatt ccttttcctt ctataagaat atctttcgcc gagagaacat
     1081 tcaaaatcgc tgctggccat ttcatttcta cgccggctac tcgtgtatcg atttctggaa
     1141 atgcttcgat atctgttctc cccttgagga ctgcaccttc ttcaaagata agatgactct
     1201 tatcttttaa aaaaagggct ccgactaaat aaatgccttt tggaataaat acaggtaagt
     1261 gttctttcgc accttgatcg attgcttttt gcagtattat tgtatcatct tgttgagtgt
     1321 cgtttgtttt aaagttcaaa gcattgataa tccctttttt tgtccgcaat acgtttctcc
     1381 ctatctttca ttgatttttt tcaattcttc accaataaat acagtttttg aacgattcag
     1441 tgggtaacct actagcaaaa tgattgcgga tacaaataag cagaatgctg gtaacaagtt
     1501 tgccatcgta taaatccttt cttgtactaa aactgattgg atggctccac ctgttgtaga
     1561 cgattggtaa ccaatgagtg ccagcataaa tccgcctacc gctcctgcac atgcttgtcc
     1621 tacttttctc gcaaaagaat ttactccata tactgttcca tcttctctta agcctgtctt
     1681 atattgatga taatcaatca cgtcagtaat gaatgcccag accatcaaat tgaataaacc
     1741 agatcctaaa gtagcgataa ataaaagaat cagatatacc catgcatttg ttatatgaag
     1801 gaaatacatc agcaagtaca tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt
     1861 tttaccaaat aattttgtca gctttgtagc aaatggtgct aaaactacta ctgttccgta
     1921 agtaaataaa agagcaacag acattgcttg tttattatgg aagtaatcat tgaataaata
     1981 agtcatattc gtcccagcta aaatctgatt gatcacaatg aaaatgtcaa caatgactaa
     2041 aataatcagt gctcgattag ctaacaaacc ttttaccaac tgagaagcag atactttttc
     2101 gtttttgaca actcttactc tttcagtagt caatttacaa gttaacatat atgcgacaaa
     2161 tgctattgca gcacatccga ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg
     2221 tccactcttg ttatgggtat acattaaaat cggaatgaag aatcctgtag ttgatccgcc
     2281 aatcgcagaa ccaatgcttc ggaacgtgga caacgaggtg cggtcattag gatttgagct
     2341 aattgctgct gccattgctc catacgggat attgattgtt gagagaaaaa tcccataaac
     2401 aatgtatgaa acaaaaatat aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg
     2461 caaaaacaaa atgacagtca ctaagcaaaa tggatacttc atttttctga tccaaggaat
     2521 aaatcggcca gtttctgtta actctgagac atcacataat cttccaacca tcacatcagc
     2581 aaacgcatcc aaaattctcg aaatcaagaa taacgtacct acgattgccc ctgaaacgcc
     2641 aagaacattc gtataataaa tcatcaagaa actattgact aaacctaaaa taaaacagtt
     2701 acctagatca ccgaacatgt agcctatctt atcggaaatc ccaaattcct ttttttcttt
     2761 ttttactgta tttgctgtaa cagcattttg tgaaagcatt tgaattcctc ctacctcttc
     2821 tagtaaacgt tttcaaaatt ataactcttt ttttcttgtt tttatattca caatgttggc
     2881 tttttat
//
LOCUS       DAJSKS010000206         2887 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJSKS010000206 DAJSKS010000000
VERSION     DAJSKS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.215"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            14..1141
                     /locus_tag="ON396_002710"
     CDS             14..1141
                     /locus_tag="ON396_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3114748.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="ON396_002711"
     CDS             complement(1359..2501)
                     /locus_tag="ON396_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3114749.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2506..2691)
                     /locus_tag="ON396_002712"
     CDS             complement(2506..2691)
                     /locus_tag="ON396_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCR3114750.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
BASE COUNT          955 a          528 c          453 g          951 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga
     2521 aatatggaga acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata
     2581 aaaaaagtat cctttctcaa ctaaaagttc tttacattcc ttgaagattt tattagcata
     2641 gtactgagaa aaacctagat taatcaaatc ttttcgagta gctgttttca ttatagcacc
     2701 ttccttactt tttttcattt atttctatca gctgcttttg tcaaaagcac ataggaattt
     2761 cacctctccc caagtttacg gcgagcctta cggaagtatc attatcccaa ctttaaatca
     2821 tcgcttttta ttgactataa aaattatttt agaatattta tcgctccttc tttacagcaa
     2881 gaaatca
//
LOCUS       DAJSKS010000207         2870 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJSKS010000207 DAJSKS010000000
VERSION     DAJSKS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.216"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            227..394
                     /locus_tag="ON396_002713"
     CDS             227..394
                     /locus_tag="ON396_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114751.1"
                     /translation="MVYLNKKQTCEFLGIDNNTLDKWIRIYNFPVIKIDGIIRFDVED
                     IKGFMDTFKKI"
     gene            468..1694
                     /locus_tag="ON396_002714"
     CDS             468..1694
                     /locus_tag="ON396_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3114752.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFKEITGISSEKFQKTFPTRQLAIKA
                     ENEMKKKIAKVLREENANSLELKGQIKFKEFYKSKWLPRYELGQTTRSHKAPSDITII
                     NTKNLFRLHILPIFGEYAMSYLNANTEIISDELTKKSKEYANIKIIKCYVRSMFDIAE
                     VLNYIEFNRTTKIIQSITAPKKVALEEKRIREGKQALSSKELTDWLDAVRDDFNDQLL
                     SFHDYTLFMLTLYLGDRKSETYALQWKYIDFENQTVRLKYALDKHTKKKFTKGRKDTI
                     IQVPEIVMTLLQEWKSVQAEQLLKLKIKQTPDQYLFSYSKPSGEVNCPVHTDYLNYRI
                     NSIKKRHPHLVHLTPHKLRHTYATLARQGGADMNQISNALTHSGISTTKIYVNTPDLV
                     DKAVFEAFQRGLQKSN"
     gene            complement(1852..2469)
                     /locus_tag="ON396_002715"
     CDS             complement(1852..2469)
                     /locus_tag="ON396_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3114753.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTLKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
BASE COUNT         1009 a          485 c          440 g          936 t
ORIGIN      
        1 ccctgctatt cgagctaaga attcaagtac gccatgacct atttagcgat aactacttag
       61 agatttcttt ttgttttggg aagacaaaat gccatgacaa tagcagtaga taatgatact
      121 tccgtaaggc tcgccgtaaa ctggggagag gtgaaattcc tatgtgctta cccaagtcaa
      181 ttcaaaaatt ttagttcatt tataagggga tgattttatt aaatcaatag tctatctaaa
      241 caaaaaacaa acctgtgagt ttttaggaat tgataacaat actttagata aatggattcg
      301 tatatataat tttcccgtaa taaaaataga cggtataatt cgctttgatg tagaagatat
      361 caaaggcttt atggatacgt tcaaaaagat ttgaatttta cttttgactg catatactaa
      421 aagtagcgaa atatactgcc ttactttttt attaaggagt gaggaagttg cctacgaagc
      481 tttcaaatgg aaaatataaa actaatttga gatatccaaa aaaatttaaa gaaattacag
      541 gtatcagttc agaaaaattc caaaaaacat ttccaactag gcaattggct attaaagccg
      601 aaaatgaaat gaaaaagaaa atcgcaaaag tcttaagaga ggagaatgca aattcattag
      661 agctcaaagg tcaaataaaa ttcaaggagt tttataaatc taaatggctt cctagatatg
      721 agttagggca aactactaga tcccataaag ctccaagcga tataaccatc attaatacaa
      781 agaatttatt tcgattgcat attctgccta tatttggtga gtatgccatg agctatctta
      841 atgcaaatac cgaaataata tcagatgaac tcacaaaaaa gtcgaaggaa tatgcaaaca
      901 taaaaatcat taaatgctat gttcgttcca tgtttgatat tgcagaggtt ctaaattata
      961 ttgaatttaa tcgtacaaca aaaataattc aaagtattac tgcacctaag aaagttgctc
     1021 tggaagaaaa aagaatacga gaaggaaaac aagctttatc ttcgaaagaa ctcactgatt
     1081 ggttagacgc tgtaagagat gatttcaatg atcaattact ttcctttcac gattacactc
     1141 tttttatgct tactttatat cttggggata gaaagagtga gacctatgct ttgcaatgga
     1201 aatacattga ttttgaaaac caaacagtcc gtcttaaata tgccttggat aaacacacaa
     1261 aaaagaaatt tacaaaaggt cgaaaagata ctattatcca agttccggaa attgtaatga
     1321 cactattgca agaatggaaa tcagtgcaag ctgaacaact attgaagttg aaaattaaac
     1381 agacacctga ccaatatctt tttagttact ccaaaccttc tggtgaagta aattgtccag
     1441 tccatactga ttacttaaat tatcgaatta attctataaa aaaaagacat ccccatttgg
     1501 ttcatttaac tcctcataaa cttcgacata cttacgcaac tttagctaga caaggtgggg
     1561 ctgatatgaa tcaaatttct aacgctttga cacattcagg tatttcaact acaaaaattt
     1621 atgtgaatac acctgattta gttgataaag ctgtgttcga agcgtttcag cgaggattac
     1681 aaaaatctaa ttaatgtgtt ctagaatgtg ttctaaatat aaaaaaaaga cactttccaa
     1741 ataattggaa agcgtcttga acgttgatat ataaaggata ttaacgtttt gagaattgag
     1801 atgctttacg agctttctta agacctggtt tcttgcaaaa ttatattagc ctcaactgaa
     1861 agatttattt ttttgctccc taacttcatt agtaatacca tgtttcattc gatatcttct
     1921 aaacgttcta cgattaatcc ctgttagctc ttcaacttct ttatctgtag atccagtcaa
     1981 aaacaaatta aatgctaatt gaagtcgcgg atcattctca cgatatttta gcgtacctcc
     2041 cttgtatttc ccttttgctt tagcgagtgc aattccttgt tgttgcgttt cgcgaatata
     2101 ccgacgttct tcttcggcct ggtacttgta tagttcgata attaagttat taagtaatcg
     2161 acgtaaatta tcatcagtga tacctgttaa cgctggcaaa ttcaaaactt ccagtgtagc
     2221 tttcttcagc tgaattaaat tcatcgtttc tgttagctct ttattatttc gtccaagtcg
     2281 ttttaattct gtcacaacga caatatcacc ttcacgtatg aatttcatca tttcttgaaa
     2341 tccttctcga ttggtatctt tcccacttgc tttatcacta aaaattttat cacactctga
     2401 taataattct aattgtcggt ctaaattttg atctaagctg cttactcgag catacccaat
     2461 tttcgccata ataaaactcc tttaaaattc tgaaatccgg atcattttta aattctgttg
     2521 cccaactctg ttgtcatttt tctagataaa tggcataaat tttcagcgct aagtgacaaa
     2581 agcaactaat ttttcatgat agatttttaa atatctcaat ctatctccaa aattagttgc
     2641 ttttgtagct atagttgact atgaatatga caaataatta gaaaaaactg tgccaattaa
     2701 atataaaaat gacactcatt tacattcaat agaacaccaa gagcccaaca gggtatactc
     2761 tggtgtcctt ttaaaaaagt aactttctga cctaattaat tgagctacta aaaaagctat
     2821 gaaatgagga ataaaaaagg gatcaaaaac ttttaagttt ctgacccttt
//
LOCUS       DAJSKS010000208         2728 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJSKS010000208 DAJSKS010000000
VERSION     DAJSKS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.217"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..172)
                     /locus_tag="ON396_002716"
     CDS             complement(<1..172)
                     /locus_tag="ON396_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114754.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQ"
     gene            complement(173..1030)
                     /locus_tag="ON396_002717"
     CDS             complement(173..1030)
                     /locus_tag="ON396_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR3114755.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(1315..1601)
                     /locus_tag="ON396_002718"
                     /pseudo
     CDS             complement(1315..1601)
                     /locus_tag="ON396_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1591..1920)
                     /locus_tag="ON396_002719"
     CDS             complement(1591..1920)
                     /locus_tag="ON396_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3114756.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(1997..2269)
                     /locus_tag="ON396_002720"
     CDS             complement(1997..2269)
                     /locus_tag="ON396_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR3114757.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(2269..2532)
                     /locus_tag="ON396_002721"
     CDS             complement(2269..2532)
                     /locus_tag="ON396_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR3114758.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT          810 a          543 c          365 g         1010 t
ORIGIN      
        1 tctgtctttc aacattggag gaagttttta tttcttgaac ttggtgcact tctaattgct
       61 tattttcaga tggtgtaatt gcttgctgct ttttctttgc ttctgcttct ttttttattt
      121 tggcgagtcc tttgttgttc ccaaaaatat caagtccttt gatgtcagcc aattactcca
      181 cctccgcata ccaattctcg tgttcttcca attctttcaa aatactgata aataattcgt
      241 ggcttttgcg atcccattgt tttaagagac ctttctttgt cttctccatc gtaatacctg
      301 atccgtcgta tcgttttaag cgcgcatttt tgagtacaac atttttaatc aaatgttccc
      361 cataaatctc ttgggcttgt ttatacattt cctgatctac tgcgtcaccg ctttccaaca
      421 tgaaaggaat aacaccagca acatataaat ttgtatcaaa ttctttcaca aaaaagtcca
      481 tatactcaag atattgcatc gcatttctca tcgattttac ttgggtctgt aagacgacga
      541 ttgcccagtc cgcaatgtac atcgcggatt tactatatac agaaatagaa ggtgggacat
      601 ctaaatagat gacgtcaaac tcatcaaata atggttctag cttttctttc atatatttga
      661 attgggataa tgaatcatta tattggtctt tgatccaatc atcatacatt tcaaaggaca
      721 tgtctgctgg aagtatatag aggttattga ttactttata gacagcctct ttaggtggaa
      781 gattgtttat caacacgtca gtcacagtat tttcgtaaac aatcggttca ccttctccgc
      841 ctccttcttc gttagtgaag ttcatatgcg ttaagttcat cacatcagaa gtatttcctt
      901 gcatatccaa gtcaattatc aaggttcttt ttccttcatt aactgctgat tcaaacccta
      961 acatttgcgc gcacgtactt ttacctacgc cacctttaaa attaccgatt acatatttct
     1021 tcgctaccac gttcctacct cttttctttt agcaatattc atttgatact attataacat
     1081 atttattttt agttactacc aaaaaaaaga tatatatgta ctaccttata attaataagt
     1141 aaaaggtagt acataaataa ggaaaagctg aaatatattg atttatagct ttcctcaaag
     1201 taatgacgtt atttaattta tcgagaagca gagaatactc gcgactttag tcatgtagac
     1261 gaatcagatt ttcgataaaa agaagccgtc cttcttaaga acgacaacaa atcactagat
     1321 taattttttc aactgttcat aaaagttttc atgcgttcca cataacaata ctggaataac
     1381 cttgttttct ataatctcgt aagctactct atatgttgtg cccgcatatt tgaacccact
     1441 agaccaaata cctgataaat ccccagtttt ttgttcaccc ttatatggat caatcgcaat
     1501 ttcatcataa attaaattca aaaatttttc tttcaatggc ttctctttta gctttttaaa
     1561 tacttaatga catgttttga aggaacgatt tcataagcca atttctgttg ccaactcctc
     1621 tttagtcatt actttagaat tagtaagtgt atcttcagca atatctctaa aggaagaatg
     1681 cattttgttt tttctattct taaattctac aagaatctca tctttatcgt agccttctgc
     1741 aattatatca gacaaaatat cttcgctaaa atcataaaat tctttttgtg gttcaacaaa
     1801 ttcataaaga ataccattct ctaccaactt tgcttcaact tcgactccat taggaacatt
     1861 aaaatcttta ggtactgtca acatgattga attgccttgt tttcttgttt ttgtaatcat
     1921 ttaaatccct catttctaac agtatgtaat tacttagtaa atacattata ccacgagata
     1981 atgtatttac aagtaattac tgtgactcgt aatgcgtcct tgctgaataa atttcaacca
     2041 cgtgttcatc ttcatctacc ttataaacaa cccggtgttg tctattcaat cttctagaat
     2101 acctaccttc atgtgtaggt tttaacttct caaatgattg ggtagggaga tagggatctt
     2161 cctttaaagt ctgaataacc ttctcaaatt gaggtttcaa attagactgt ttgattttct
     2221 ttaaatcagc tttagccgag tttttaatca tcacagaata gttattcatt ataagttatc
     2281 ccaatcaatt tcatcaacat tagtaaatcc actatcatct ttttctcgct tacgaacaac
     2341 acccaaagtg cctatttgct caagtaatag cgtttctttg attgctttcc aatcatccaa
     2401 accaataagc accgcattat tttcactgcg ctcactaatg atttcaattt ccgtatgatt
     2461 ctcatttaca tctttcaaca acttataaaa atttttccgt gctgagctag gatttaagac
     2521 tgtttttgcc atatctactc cccctctaat ttatatctcc attctaacgt acttaattaa
     2581 gtacgtaaat tcgattgttt tgtttaaatt ggagtaccaa aacagcattg taaagtgtta
     2641 tccgtttcct ttatacccgt ggtgctagtt aattttttcc tagaggaaaa tgtctaaaca
     2701 tgttggtaaa tcaacaaaaa aacgcata
//
LOCUS       DAJSKS010000209         2692 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJSKS010000209 DAJSKS010000000
VERSION     DAJSKS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.218"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..472
                     /locus_tag="ON396_002722"
     CDS             <1..472
                     /locus_tag="ON396_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114759.1"
                     /translation="KNFNQKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLEN
                     KEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPV
                     KEQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            647..1480
                     /gene="msrA"
                     /locus_tag="ON396_002723"
     CDS             647..1480
                     /gene="msrA"
                     /locus_tag="ON396_002723"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR3114760.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1593..2549
                     /locus_tag="ON396_002724"
     CDS             1593..2549
                     /locus_tag="ON396_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR3114761.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT          989 a          429 c          524 g          750 t
ORIGIN      
        1 aaaaaatttc aatcaaaaaa taatttctca gccgggacat attgttgtaa acaaagaaac
       61 agtagaggcg tataccacta aaggaatgga ttatttttta tatgattatt ctcctatcgc
      121 ttcattggag aataaggagc atataataaa acacgaagca aagattggca gtcaatgggt
      181 aaaccttcct tataaaacaa ctggtagttc tatcatatat agagtaacta atgagaagag
      241 ccaagtaatc acacttcctg tatatgctta tttaggaacg aatgtagaaa taaatcatca
      301 acctgtaaag gagcaggtga atgctaatgg attggtacaa ataagcggag tcaaaggagt
      361 tgctgatatt aaagtaagca caagttatac agtaacgact attatttctt atcttgttac
      421 attaactgca tatttatgtt tattttttaa aatagttttc cataaaaggt aaattaactt
      481 ttcatttata aaataaattg attaaaaagg agaatctgtc gataaggatt ctctcttttt
      541 tatacgaaaa atctgtaggg ggatgaagta atcgcttatt tcctcacttt atgataaatt
      601 acagctaaag tcactagctt gtcttcttcg gaaaggagga gaatctatgg atgaggaaca
      661 aatattcatt caaacacttt ataatctgat tttgaatccg aatacacgag attgggaacg
      721 aaaagtattg attcagacaa agaatgacat aagagaaaat atatccgtta aagaacagtt
      781 atcaaaacta gaagcaacac tacgtccatt agctatccga atgaatctga cgcctgatgt
      841 aatggacttt tatttattga ttacggaagg atttgataag gagcaaaaat atgacttttc
      901 caagcatgcc atgcaagatg ctgactatca agaacgtgca gtatttgcag ggggatgttt
      961 ctggtgtatg gtcgagccgt ttgaatcgaa aaaagggatc ctatctgttt tgtcaggata
     1021 cacgggcgga catgttgaaa aaccaaatta tgatcaagta agtggtggtt acacaggtca
     1081 tgtggaagct gtggagatca tttatgacac aagagaaata agttatagcg agttgctgac
     1141 aatctattgg caaattactg atcccacaga tacatttgga caatttcaag atagagggaa
     1201 gcaatatcgt ccagtgattt tttatcaagg cgaacgacaa aaagaattag cggaacaatc
     1261 aaaacagaaa ctagacagtt caggaacttt ccaccaacca atcgtcacaa aaatcgaacc
     1321 agccggtacg ttttggccag cagaaaacta tcatcagcaa ttttacaaaa aacaaccgaa
     1381 acgttataaa aaaatccagc aagcacgtaa tcaatttctg atctatcaac gagtaaaaaa
     1441 caaatggcaa aaaaatatac gaaaaaatca ttttgattaa ctccttttct ttaaaatgta
     1501 gtaaactaaa aatagaaaaa agaaagcgtt ttaaaaagat ttgatgagga ggagaaaaat
     1561 tgtatattgc agatagtgca cgttatgaaa aaatggaata tcgtaaatcc ggtcattcag
     1621 gtttgaaatt accgatttta tctctcggat tatggcaaaa ttttggtgat tatgatccta
     1681 tccataatca aagagagatt ttaagaggcg cattcgacat gggaattacc catttcgacc
     1741 ttgcgaataa ttatggaggc cctgctggag ctgcagagaa aaactttggg cgtattttta
     1801 gagaagattt tcaagcctac cgtgatgaac tgattatttc tagcaaagca ggttatcata
     1861 tgtgggaagg gccatatggg gaatggggct ctcgtaaatc catcatttca agttgtgacc
     1921 aaagtctcca aagaatgggg ctagattatg ttgacatctt ctatcatcat cgcccagacc
     1981 ctgatacacc tttggaagaa acagcagaag ctttgatgca acttgtaaga caaggaaaag
     2041 cattatatat tggtatatcc aactataatg gagaagatac aaaaaaaatg actgaaattt
     2101 taaaaagaaa ggaagcaccg ttcattattc atcagatgcg ctacaatatg ttttctcgtg
     2161 cactactaga agacgatcta tctccagtat tagaagaaga agggctaggg gcaatcacgt
     2221 tcagtccgct tgctcaaggg ttgctgacaa atcgttatct acatggtatt cctgaagatt
     2281 ctagagcaca tcgtaaggaa ataccattcc tttcagaaga acaagttgga tcaactctgg
     2341 aaaaaatcaa agcactgcaa acaatcgccg tatcacgagg acaatcatta gcgcaaatgg
     2401 cccttgcatg gaatttgcga caaaaatctg ttacaagtgt actggtcgga gcaagtcggt
     2461 taagtcagtt acaagaaagt gttcgaatga tggataattt agatttctca ccagaagaag
     2521 aattgagaat cgaccaaatt ctagaataga aacgatgtaa tcaaactttt tatccatata
     2581 ttaaaaaaaa caaaagatga catcaagaat cgaatgaaga tcgtctgtta ttgaacatca
     2641 tattttttgt cgttcaaaat aaaaatgatc gaaggctagg agtgaatttt at
//
LOCUS       DAJSKS010000210         2692 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJSKS010000210 DAJSKS010000000
VERSION     DAJSKS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.219"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            64..1149
                     /gene="xerS"
                     /locus_tag="ON396_002725"
     CDS             64..1149
                     /gene="xerS"
                     /locus_tag="ON396_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HCR3114762.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(1433..2098)
                     /locus_tag="ON396_002726"
     CDS             complement(1433..2098)
                     /locus_tag="ON396_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3114763.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(2252..2401)
                     /locus_tag="ON396_002727"
     CDS             complement(2252..2401)
                     /locus_tag="ON396_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR3114764.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            complement(2394..2603)
                     /locus_tag="ON396_002728"
     CDS             complement(2394..2603)
                     /locus_tag="ON396_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCR3114765.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
BASE COUNT          923 a          477 c          378 g          914 t
ORIGIN      
        1 ataattggta tagtccaatt atagagatac tcttctcccc tacttattga aaggatgaat
       61 gacatgaaaa atacagaaat atatcaaaaa atagatacaa tcatacttca tatgcccgat
      121 tatataaaag attttgttca agatcgtgag gataaagatc aatcacctcg aaccacttta
      181 gaatatctaa aaaattataa gctcttttat gaatggcttc tgagtgagtc tatcgttcct
      241 gatacttcta tcacctcgat aagacagtta actgcctatg acctaaactt gtacaaatct
      301 catttgaaac gtcgcacaaa agaaaatgta aaaaaagaga caacaaaaac taagctcaaa
      361 gataataata acttaggtct atctacttct actatcaata gaaatatcac ggcattaaag
      421 gttctattta aatatctttc gaaaagttcc aataatcctc taggaaaacc atacttagac
      481 aataatccta tggatcaagt agcaaccatc acagataaaa ctacgttagc tgcacgtgcc
      541 aatgccattg agaaaaaatt atttctagat aaggatacac aaaattatct agattatatt
      601 gccaatgaat ataaaaatac tttgagtaaa cgagctttga tctattatca acgagatgta
      661 gaaagagacc tggcgatcaa tgcactgatt ttaggatctg gattgagact ctccgaggtt
      721 ataaatatta atttagatga cctatcttta gataagaata atgtggtagt aactagaaaa
      781 ggaaataaaa gagatgctgt caatatagca gcattcgcaa tggaatattt agctaattat
      841 ctagctattc ggaaagaacg atataaagta acagaaaacg aaaaagcttt atttctagca
      901 atttaccaag gagaagccaa gcgtatatcc ggtattgcaa tcgaacggat ggtagcaaaa
      961 tattcaaaag gctttagaat tcaagtcagc cctcacaaac tgcgtcatac attagctact
     1021 agactctacc aacagacaaa ttcattggta ttaactgccc aacaattggg acattcaagt
     1081 accaatacaa caactttata tacccatata gacaatgcgg ctacgatcga tgcattaaat
     1141 gctttatgag tataatgtat aaataggcgg aaataagaaa caacacgcta gaaaagcctg
     1201 tattatagtt tttctagtgt gtttcttttg gtgtgatcct acttttgttt atcatttatt
     1261 ttttattatc gtaaataggt gtcggtttga ttttagaaaa attagtttgg atataatctg
     1321 tgggatcatt tgttatctct ctcaatgaat ttagatacaa atgaccataa gttcctaaag
     1381 tcgtttgtac atcagcatgt tttaaccttt ctttaattcg aagtgcatct ttttattgtg
     1441 atataagcaa agaagcatga ctatgcctta atccgtgtat gcggattata tgaacaccaa
     1501 cgatcgttgc atgtcttctc ataatatgac ggattgtata tttctgagta ggtgttccat
     1561 tataagaaag tacaaacgac atgcctccta ttgaaacttg acgctccttc caagctttta
     1621 aaaaggtaat tgtattgtcg tctagttcga ttgtacgaat actagaagcc gtttcgggtt
     1681 ctacaaactc ataggttttg gctctcttat aatagagtgt tttattcaca cgcaagtttt
     1741 tattttcaaa atcaatatct ttccaagtta atgcagtggc ttctcccaaa cgcataacgg
     1801 tcataaaaag taaccacata caaataaaac caaaatgttg gtacaaatca tcaatataaa
     1861 aagttgaaat aacttttgta aattcttctt ttgtccaaaa atcaactgta gttttccttt
     1921 tcttcacatt tccaataact tttgcaggat ttttttcaag tagtcccaaa acaactgctc
     1981 gatctaatga catctgaaaa atcccataca tgtttcttac ataagaattt ttatatttgc
     2041 tcaataaatt attttgccac ttttgaatgt caattggacg tatttctctt aactccattt
     2101 taaagaaata agaaaagttt ttttgattgt agataaccga tttttatatg ttctttcttc
     2161 tactttccct tgataataag gtaggaataa tttataaaaa aactcctcaa acaacatagt
     2221 actttgagaa gtttctgctt cttttccata attagaaaca agttcggtat acgctttttt
     2281 tcttctttaa ctgttttaac ccctctacgt ttttttgtac tcttttccct gttcgtttgt
     2341 cgaatcccaa actagcagta aaatgatatt tcccattttt atctttttta aaattaacca
     2401 tgattctgaa aatcctcctc tacctctatc ttcataccta aaacttcttc tacagattca
     2461 acaggtacta gtcctaatct tctattttcg taataggtat agctttttga aaccataatt
     2521 gcttttgcct gtcgtatgat tctctcagac tgatacttcc cataacctaa tttcattaat
     2581 tcattttttg ttataaccac catttaacca ccacattttt tattcaaata taaaaaagaa
     2641 cactactaaa aatagtagcg ttcctgcata tttttattca aatttaatca gc
//
LOCUS       DAJSKS010000211         2667 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJSKS010000211 DAJSKS010000000
VERSION     DAJSKS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.220"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            751..2577
                     /gene="ltrA"
                     /locus_tag="ON396_002729"
     CDS             751..2577
                     /gene="ltrA"
                     /locus_tag="ON396_002729"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR3114766.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          955 a          466 c          593 g          653 t
ORIGIN      
        1 aaagggggtg cgaaacgttc ctacttgaaa agagtaggca cagcacttca ttaaatcaat
       61 atttaatgtg tgttggagaa ctaaacacac gaatagtcga atgacggggt aacgccctga
      121 aaggctactt taatactctg actggcagaa acttgagtaa acaaggctct gtcagaagct
      181 cggtgaagtc ggcagacaga caccgtaaca gataatgctg acgaaagatt cccagaggtg
      241 ggcgttgtgt cctatatgcc ggaagtctat aaaatatcta tggttagaat gtagatgaga
      301 ggtctactga cgaatccttg aatgtacagg tctatatcgg ggctggatag aaatgtccag
      361 tgacacaaac agtgtcgcta ctgtgggtga gtaagactcc gcatttatga aaatccatat
      421 aacgttaaag gcgttatcaa ggtagcaggc ttatagagga aacctaagga tatatgtaca
      481 gatagtgtgt ttggaacgtt gaaagctgac aacatggaga ttttactgtc tgtgaagtgt
      541 tcacatcaga aagttattgt gagattagca atgatataag gtgtgttaat gtcagtgaga
      601 gtggtggcac agtacctatg aaaccgtgat aacaagcggt ggagggatag ccactagtca
      661 tatgaagaat agaattacta agaaaaatgg tatgggttcg agtaagacta agagatgtac
      721 ttgctcctgc aaacaaggag ggtacagacc atgctaaaga aaaacaagct ccgctataac
      781 gagtattatg atatgcaaca tatttatgac gagttatacg cacagagcaa gaacggtaat
      841 aacttttata agttacttga aattattggt tcggaacaaa atatttgttt agcttaccgt
      901 aatctgaaat caaacagtgg cagtaaaacg gctggaacgg atggaatgac gatagacgat
      961 ataaaacaac tcagcaatgc tgagattgtt gctaccgttc gggaaagcct tagcaactac
     1021 cgccccaaat cagtcagacg tgttttcatt ccaaaagctg ggagcgataa aatgcgtcca
     1081 ttggggattc cgtgtatctg ggacaggctg gtacaacaat gtatcctgca agtcctagag
     1141 ccgatatgcg aaccaaagtt ccataatcat tcctatggat tccgtgcgaa tcgaagtgcc
     1201 caccatgcag tcagcagagt aacgacactg ataaatttat caaaatatca ttactgtgtt
     1261 gacgtagaca ttaaaggatt tttcgataat gtcaatcatg gaaaactatt aaaacaaatc
     1321 tggacacttg gtattcggga taaaagattg atttgcatta tcagcaaaat gctgaaagcc
     1381 gaaattgacg gcgagggtgt cccagaaaaa ggaacgccac aaggcggtct gttaagtcca
     1441 ctgctctccc tcatagtcct taatgaatta gactggtggg taagcagtca gtgggaaaca
     1501 ttccagccga agaaccgcag taaaaatggg tggcttcaat acgcaaagaa atataccaaa
     1561 ctgaaaagcg gttttattgt acgttacgct gatgatttta aaatcatgtg ttcaacctat
     1621 ggagaagcac aaagatttta ccacagtacc gtagattttc ttaataaaag gctgaaatta
     1681 gaaatcagtc cagagaaatc aaaggtagta aatctgaaga aaaattcatc agattttctg
     1741 gggttcaaaa tcaaagtcat tccaaaagga agaacgaaac acggttatgt tgccaaaaca
     1801 gatatgaacc aaaaagcatt aaagaaagcg aaaacaaatc taaagttaaa ggtaaaagat
     1861 atagccagac atacgactgg cttcaatatc agcagatata atctgacagt tatcggtatg
     1921 cagaattact actgcatagc aactaacgta tataacaacc tgacagaagt aagttacgct
     1981 ctcttaccga ccatacgaat ccgtttaagg aacattgcaa aatcagttcc atttgaaagt
     2041 acaagttcag aatttcagag caggacaaaa ggaatcagac caaaaacaaa aatagtgatg
     2101 attgcggata atccgctatt gccgattcaa ggagtacaac acaaaaatcc tatgaatttc
     2161 tcgcaagata tttgtaattt tacaaagcaa gggagaaaca aggtgcatga ggacgttgta
     2221 gttgtaacaa aagaagaaat ccgagcgttg ttggaaaatg aaaaccctgc cgatagcgta
     2281 gagtttaacg acaatcgtat ttcggcatat atcgcacaac aaggcaactg ctatgtaatg
     2341 aatcgtcgag gaactccgtc cactctgata tgtattcaca aatcagacaa aggagataac
     2401 cttgacagat attctaatct ggcatttgtg gaaattccca ttgcaaaggc aatcctaaca
     2461 gaatcagcag acgaagcaaa atcactgtta aaaggttatg ttcttaatag tcagcagaag
     2521 aaaaaactga acagaatacg gacgaattac ggataccaga caataactgg gaaataactt
     2581 ataaatctat ttggatatga tggaacgccg tatgaagcga aagtttcatg tacggtgtga
     2641 agcgggggaa aacttggaga taacatc
//
LOCUS       DAJSKS010000212         2659 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJSKS010000212 DAJSKS010000000
VERSION     DAJSKS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.221"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            68..442
                     /locus_tag="ON396_002730"
     CDS             68..442
                     /locus_tag="ON396_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3114767.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            447..875
                     /locus_tag="ON396_002731"
     CDS             447..875
                     /locus_tag="ON396_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCR3114768.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            925..1578
                     /locus_tag="ON396_002732"
     CDS             925..1578
                     /locus_tag="ON396_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114769.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            1714..2280
                     /locus_tag="ON396_002733"
     CDS             1714..2280
                     /locus_tag="ON396_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HCR3114770.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            2396..>2659
                     /locus_tag="ON396_002734"
     CDS             2396..>2659
                     /locus_tag="ON396_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3114771.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKE"
BASE COUNT         1006 a          439 c          517 g          697 t
ORIGIN      
        1 aattgtcaac caaaaatata acttttaagc tatttatttt cttttcagct ttgaaaggtt
       61 gataataatg agtttagtta ctaagattaa agaattagca gacgaaaagc atgtgactat
      121 agcagaagta gaaagacagg tgggcatctc taatggacaa ataagaagat gggataaagc
      181 ctcgccaaaa tctgagaact taaagaaagt tgctgattat tttggtgtca caactgatta
      241 tttattggga aataataatg ttcccaagtg ggctacaaaa gaggaagtgg ttgaacttga
      301 taaactacta gactcaaatg ttaatatgtc ttatggtggg gaaacattga cacccgaaca
      361 aatacagcgc gtaaaagata tcctgatagc gactttctgg gatattgtga aagaagacaa
      421 agaaaaaggc aaaaagatgt gagcttatgg agatggatac gattaattta gtcgaggagt
      481 tgaagcggaa ataccagtcc gctaatcctt tttatatttg tgaaaagatg ggcattaaaa
      541 ttcaatacgt tccttttatc gagaatccca aggggcagtt tcaagaaatt agagatcgtg
      601 cgataatctt tttaaatgat gaactgcgag actctgagga aagattctac atttgcgctc
      661 acgaattagg tcacgctatt tttcatcgtg gcttatccag ctattatgta tcaacaagaa
      721 catctagaag caaatctgaa agcgaagcta attgctttgc tgctaatctc attgtttctc
      781 tttataaaga agataatgat caatatccta aaaaaatcga ggaattaaag aatctttacg
      841 ggcttccaga aagcgcttat cgttttctta tataaaaaag cccgtgttag cacacatatt
      901 acaacgagaa agaggaatta taaaatgaaa aaagttagcg ttatgttgtt gttaagtact
      961 gctctgctac tttcagcttg ttcaaataat aaaaaagctg aatcaacaga taccacttct
     1021 aaccaagaaa caaaaataag taaaacaaaa gaaacaactg aaaccagttc atctactagc
     1081 aaatctacat ctaaaacaga ttctagttca acagttacaa gctccaacca agctacggcg
     1141 gaacctagcc caacagttat aagctccagt cagagtacaa tccaaaccgc acctcaagaa
     1201 gaaacatatg aacagatgaa acaacgcact ttacagtcaa ctccagctga tcgtgcaaat
     1261 tggtccaaca aagagtggga agctttcggc gtggcccttt atgaaaatgg attgactaca
     1321 gatgatgctg gcaatattat cagtcaagat cagaaagaac aacaagcagc atctcaacag
     1381 aatccagaag accaacaaac aagcgctcag caagacgctg acactttatc acttactgat
     1441 tttgttaaca aatacgggat gtcgcctgtt gcatggaaag tacagaatgg aatgtctgaa
     1501 gaagaagcat tgcgtacaac acagcaaaag acttccggtg aagttcaatt aggattttct
     1561 aaatacggaa ttcaataata catttttatg ccctactatt ttgcctataa tctctaaaaa
     1621 agttataaag aaaaaagccc gtgctgcaac acggactcat acctcatttc tgagatcaca
     1681 aatatattat aacaagaagc gagggatatt taaatggcaa aaaaagttat gggtcaagat
     1741 gggaaaatgt ataaagttaa gaaaccgttt tataaacggg tatggttttg gttgttagcg
     1801 gttgttgtgg tgtttattgc tataggttcg caaggaggca gtgatgatgc taaaaatacc
     1861 gtcgctgaaa caactaaaga aagcgtgaca gaagtgtctt ctgcagaatc ggtagcagaa
     1921 tctacagtcg ttgaagaaga aactgaaact actgaaacta ctatagaaga agttactcaa
     1981 gaagaaagtg ttcctcgtga atacagaaat gcattgagca aagctgaaag ctatctaggt
     2041 tgggctggta tgtctgaaca aggtttgcgt gaacaactag agtttgaaga atatccaagt
     2101 gatgcaatcg attatgcgct ggctaatgtt gatgtcgatt acaacgaaca agctttggct
     2161 aaagcggaaa gttacgatga ttgggcatca atgtcagatt cgcaattgta cgatcaactt
     2221 atatttgaag gttttacaaa tgagcaagca caatacgctt tagataacct accacaataa
     2281 ctaacaaaaa cacgcccctc cctcgccaaa gtttgtggac gtgatgaaaa ataaacctgc
     2341 tataatcggc ttacttatct attcctatta tagcaacaaa taggagatga aaacaatgtg
     2401 gatcgaaact aaaactgata aaaacggaaa aaaagtatat aaatataatg agcgatatat
     2461 tgatccaaaa actagaaaaa gaaaaaaagt gagcattact tataaaaata aatctcgaga
     2521 aactcagaaa gtggcattgc tagagttaaa taaaaaaatt gatataaaat taaacgaaaa
     2581 aacacttcat aagcctgatc taacatttca tgagcttgtt gaagaatggt tagttatata
     2641 caaaagacaa gttaaggaa
//
LOCUS       DAJSKS010000213         2630 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJSKS010000213 DAJSKS010000000
VERSION     DAJSKS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.222"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(61..534)
                     /gene="dfrF"
                     /locus_tag="ON396_002735"
     CDS             complement(61..534)
                     /gene="dfrF"
                     /locus_tag="ON396_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HCR3114772.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(953..1171)
                     /locus_tag="ON396_002736"
                     /pseudo
     CDS             complement(953..1171)
                     /locus_tag="ON396_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1202..2416)
                     /locus_tag="ON396_002737"
     CDS             complement(1202..2416)
                     /locus_tag="ON396_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCR3114773.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT          827 a          577 c          401 g          825 t
ORIGIN      
        1 tatatatgca taaaaagcaa tatatttatg aaaacaccct aaatccaaaa tttactcaat
       61 tcattttttc cttacataaa atgttctcgt atatttcact tcctcaccaa gtgtttcccc
      121 tatcaaaact tcaaaatcat tgatatcaaa ttctggaaag aatgtatctc catcttcaat
      181 atttaaatct acttctgtga tatacatttt atctactatt tgcaaagctt ccttaaatag
      241 tccatatcca ccagatatgt atacatctcg tcctttagcc aataataatg catcttctaa
      301 tgatttaact gaaactaaat tatctccttg atactctgtt gtggtggaaa caacaatatt
      361 cattctatta ggcaacggat gaccgatttc ttcataagac tttcgcccca taataaccac
      421 attacccgtt gttaactctc taaattgctt ttgttctccc tttattttcc atggtatatt
      481 accattcttg cctataacat tattctttga cctcgcaaca atcaaaccta tcatctattt
      541 catcctccat aatatgatgt cgtacctgtg attccatcaa gtgcaccaca tcttaacaaa
      601 ctcaaaatta tctcgaacta accagctcga caaattcctt ttttgtcttc atctgcatta
      661 tatcatattg ttataagcat tgccatttct tttctgcttt tgaaaagtta cttatcagcc
      721 tatgttttgt aaagggtgat attcagcatt tctcacgcaa caagcccttg acaaaccata
      781 gactaataag ttttaaggta atcaagcaga aatgaaatag tctgctgttt cacggcaaca
      841 aaaaagagat accaacacac ttatgtagta tctcttagtc tagtagcaat attcttttgt
      901 ttgcaagcag gtaaacatgg gctgtttctt aatatctttt tgtttgggaa acaccttttt
      961 cccatccttt caccgttgcc atctctgctg cgcacaatgc cacagggata aaccatagca
     1021 aaccgcctaa taacaagaaa aatactaagc tgaagcctga ggtcgcgaat gtgggatatt
     1081 catataatga aacgaccatt gagatggtca tagccaaaaa gccaaagaac gttaattttt
     1141 ttgcaggaat tttcgttttg ctttgttgca tgaatcttcc tcttttctat ttgaatttgt
     1201 ttcaattgag tttgttgcca aagaagaaaa agtccaatcc ttcttcttgt tcttcttttg
     1261 tagtagtgat cgttgtaaag tgcatcgccg attttaaaat cactgttgtc agcacatagg
     1321 catacacggc agtaatgatg acaccaatca cttgaatccc taagaagtac cagctgcttg
     1381 gattcgttag taaatggctg ttagatgcaa agatccctat cagaatcgta cctgtgaatc
     1441 cgcccatgcc atgaacaccc cacacatcaa gtgcatcatc ccattgtttc tttttacgaa
     1501 aatcaacagc agcatgacag acgatacccg caatgatccc aatcagcatg gcactttttg
     1561 cttctacata accggcacaa ggcgtaatgg ctgccaatcc agcaacggat cctgttagta
     1621 cgtcgacaaa atcaaagcca tttcctttta cttttgcaat gatcagccaa gtaatcatcg
     1681 caaatgcaag agcgatgaat gttgaaccaa atgcattgac tgcttgttca tctgctcgaa
     1741 gtgcaccacc ggaattaaaa ccgaaccagc caaaccaaag cagtcctgta ccgatagcag
     1801 ctgccataag actgctatgc tgacttcttt ctcctaaagg aatctttctt tgaccaagtg
     1861 tgaggataca tgctaaagag ccgaatccag ctgtcgtgtg gatgactgct ccaccagcga
     1921 aatctctaaa tcccatctga tccaaaaatc cgccacccca tatccagtgg caaacaggga
     1981 aataaatcag cagattccaa aaaataacaa gtataatata gccttttaaa ttcaatctcc
     2041 ctgcaaaagc tcctgtcatc aaaggaacgg taatcacgat aaacatcagt tggtaaagaa
     2101 agaacattaa aaatggaatc gttgcaccat gaaacatgtt ggggaagctt gtaacatgtc
     2161 tcatcaaaaa gaaatctgcc gggtttccaa taacaccgcc gatatcccgt ccaaaggcca
     2221 atccgaatcc accaaatacc cacataatcg ttgttatccc aacggagaga aaagattgag
     2281 acatgatcgt tagtatatta ttcttccgtg ctaatccgcc gtaaaaaaaa gctagaccag
     2341 gagtcatcag gcaaaccatt gctgtacata aaatcatgaa tgctgtatct cctgtattga
     2401 taggtaatgt attcattttt ttcgcctcct ttctacgatt acgagtataa aagaaggtgt
     2461 agttgttttt ttcttctgtt tttttgaatt aatacttgca tttataaaag acagacaatt
     2521 aataaaagct tgatgtgttg tgctcttttt cgtttttcac acaaataaag acaaaaaata
     2581 aacggtcttc tttttataga aaaccgttta ttttcgtctt tttttatttt
//
LOCUS       DAJSKS010000214         2549 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJSKS010000214 DAJSKS010000000
VERSION     DAJSKS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.223"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            8..1015
                     /locus_tag="ON396_002738"
     CDS             8..1015
                     /locus_tag="ON396_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3114774.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1027..2031
                     /gene="neuB"
                     /locus_tag="ON396_002739"
     CDS             1027..2031
                     /gene="neuB"
                     /locus_tag="ON396_002739"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HCR3114775.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2028..>2549
                     /locus_tag="ON396_002740"
     CDS             2028..>2549
                     /locus_tag="ON396_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HCR3114776.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAP"
BASE COUNT          929 a          331 c          511 g          778 t
ORIGIN      
        1 gatataagtg tttaaaggaa aaacaatatt agtaactggc ggaactggtt ccattggaag
       61 tgaaattgtt cgtcagcttt taaaatatga accaaaagct atcagaattt atagtagagg
      121 tgaagaaaaa caattttata tgcaacaaga gctctcaaat cacactaatt tgagattttt
      181 aattggtgat atcagagact ctcaaaggat tgattatgca tgtcgtgatg tggatattat
      241 ttttcatgct gccgcaatga aacacgttcc agcaagtgaa tataatccta tggaagctgt
      301 aaaaacaaat gtgatgggaa ctcaaaatgt aatagatgca gcgatcaaaa ataatgtaaa
      361 acattttgta ggtatcagta cagataaagt agtgagtcct actaacacca tgggtgcgac
      421 aaagttattg agtgaaaaat taataatagc tgccaatcag tataaaggaa aaacagaaac
      481 aatattttct tgtgtaaggt ttggaaatgt catggggtca agtggatctg ttattcctct
      541 ttttagtaaa caaataaaaa acaataaaga tattactttg acagatgttg atatgacacg
      601 atttatgatg tcaataccgg atgctgcaag attagttctg tctgctgcta tgaattcaaa
      661 atttggagaa acatatgtct taaaaatgcc ggcaatcaat attttaactt tagctcaatc
      721 attaataaaa atatataatg atacacatga tgaaaaatat agaggtgaaa taaaagttgt
      781 aggtatcagg ccaggagaaa aattatatga agagctaatg actacagacg aaattgaacg
      841 ctcatatgaa aatgataaaa tgtatatcat tccaccacac tatcatgcta ctgacgaact
      901 gtacaaagat tatgaaaaaa ttaaaatcac tgcatactct tcagatgcaa cagaattaat
      961 gagtggacag gaatttatta gaatcgcaaa agagaataat gtcgtgactt ggtaaggagt
     1021 tttatgatga aatcagaagt atatgtaatt gcagaaattg gtgtaaacca taatggaagt
     1081 ctagatttag ctttagaaag tattgataaa gctaaagaat gtggagctaa cgcggttaaa
     1141 tttcaaacat ttaaaacgaa taagctagtt tccaaaaagg ctccaatggc aaaatatcag
     1201 aaggataatc ttaaagagga cagttctcaa tttgaaatgc taaaaaaatt agaattatct
     1261 caatctgatt ttattagaat aaaagattat tgtatacaaa ataaaattga ttttttgtct
     1321 actccttttg atgaagaaag tgcagacttt ctaaaacaaa ttggtgtaca aggatttaaa
     1381 attggctcag gagatttaac aaatttacct tttcttagaa aaatagatat gtatgggttg
     1441 ccagttttat tatctacagg tatggctgat attcatgagg tcagtgaggc tgtagattgt
     1501 ttcatagata gtccagttac tattttacat tgtacttcaa attatccggc tagttctgaa
     1561 gatattaatt tattggcgat taacacatta aaagaaatgt tcgggatacc cgttgggtac
     1621 tccgatcatt cattgggata tgatgttggt atttgtgctg ttgcgatggg tgctaaggta
     1681 attgagaaac attttacctt ggataaggaa ctacctggtc cagatcataa agcttcttta
     1741 gatcctcagg aatttgcaga ttttgtaaag catattagaa acactgaaat atttttaggt
     1801 gatggtgtta agcgagcaat gccttctgaa cttagtacaa aagaagtatc aagaaaaagc
     1861 ctagtggtca ctgagaattt aaatgtggga gatattctta cagagaaatc tcttacaata
     1921 aaacgtcctg gaaatggaat agctcctaag tttttatatc aatatattgg caaaaaagtt
     1981 aaaagaccaa taagtgcaga tagtgttctg cttgaggaag atgttgaatg aaaaaaatac
     2041 ttatagtaag tggaacacga gctgattatg gaatatataa acctgttgct gagaatttac
     2101 tacaagataa ttgggatgta tcatttgttg tttctggaat gcatttatcc cataaatatg
     2161 gatttactaa agaattgatc ttagctgacg gatttagaat tagtggagaa gttcaaagtt
     2221 tgtttttaga gaacaataag ggaaacatgg ctcggtctgt ggcattagag ataaatggtt
     2281 tcacaaatat atttgaacaa actagccctg attttgtatt attactaggg gatagaggag
     2341 aaatgttggc agctgcgata gcttgtttgt atcttggaat caaaacagca catattcacg
     2401 gaggcgaagt gtctggtact gtagatgaat caataagaca tgccataacg aaaatagcat
     2461 ctgttcattt tgctgctacg gaaaaaagca aagagcgttt aataaaaatg ggggaagatt
     2521 cttggagagt ttttcccgtc ggagctcct
//
LOCUS       DAJSKS010000215         2529 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJSKS010000215 DAJSKS010000000
VERSION     DAJSKS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.224"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            10..666
                     /locus_tag="ON396_002741"
     CDS             10..666
                     /locus_tag="ON396_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCR3114777.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            848..1378
                     /locus_tag="ON396_002742"
     CDS             848..1378
                     /locus_tag="ON396_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3114778.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            1924..2166
                     /locus_tag="ON396_002743"
     CDS             1924..2166
                     /locus_tag="ON396_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HCR3114779.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            2163..>2529
                     /locus_tag="ON396_002744"
     CDS             2163..>2529
                     /locus_tag="ON396_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCR3114780.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSY"
BASE COUNT          931 a          332 c          504 g          762 t
ORIGIN      
        1 ggattgaata tgaataagga atggtctgaa cttaataaaa caatgcaggc acaaataaaa
       61 aagaaagata cttataagag gggtattgat actttactta ctttacgaag tcagttaata
      121 caaaccttag tatctttcaa agaagaatta tgcagagaag attttaactc aatccccttc
      181 ataaacgctg atggttatca tagtaagacg attgcttatt ctatttggca tattttccgc
      241 attgaagata tagttgtgca tacagtgata aatgaagatg aacaagtatt ttttgcaggc
      301 aattatcaag agcgtatcaa ttcacctatc attacaacag gaaatgaact catgaaacag
      361 cagattgcag acttttcaaa acagcttaat ctggaagaat tatatttata tattttcgaa
      421 gtatgggaaa gcactgagaa aatgcttgaa cggttatctt atgatgaatt gaaaagaaaa
      481 ataccgaaag aaagaaaagg atacttagaa tcgttgaatg tagtaaacga caatgaaaaa
      541 gcaatttggc tgattgatta ttggtgtaat aaagatattt gtggactaat tcaaatgccg
      601 ttttcaagac attggattat gcacacagaa gcctgtttgc gtataaagaa caagatacat
      661 tcataggcaa aaatttcagt ttgtagaact aaggaaattg gaatttaagg aggaataaag
      721 tatggttagt gattttataa gagcagcatt tccatggatt ttaatgggtt tatttgtagc
      781 aatcagttgt tcccttatga gtaaaaaaga gaaataaatt cctgtttgta tttataaaag
      841 agaaaaaatg agaaaagtag ttttatttat tgccatgagc cttgacggat atattgcgga
      901 cggtaatggt ggagtagctt ggctaaatgg tcatggaaat gacaatgaaa acatcgacac
      961 ttatacagaa tttaccaaag atatagatac tgtcttaatg gggtggaata cttctcatca
     1021 ggttgttact gaactttctc cgcaagaatg ggtatataat aaatttacaa cttatgtatt
     1081 aacacataaa gagtgcaatt cttcccaaca aattcatttt acaagtgaaa atcctgtttt
     1141 attattggaa cggctaaaac aagaagcagg aaaggatatt tggatttgtg gcggagcaaa
     1201 tcttgttcag cagttggtaa gcaaaaatat gattgataaa tattacattt ctgttattcc
     1261 tactctgtta ggaaatgggg ttcgtctttt gggcaacata gacagggaaa taaagttaag
     1321 gctttgtaaa acacagactt ataacggaat aaccgatttg atttatatgc gtagataact
     1381 agagagaaac agaaaagctg ttaaaccgac ggagcgatcc gaccacgttg gcgggaaaat
     1441 ccttaagatt tttccgccat gcttgcaagg gggcggttcg cttcggcggt tcggcgagct
     1501 tgcgagccgt tcaaagcccc cgttatctaa caaggggggc acaaaaaaca agaaacacta
     1561 tacactaaca agcggaaaac gctgtattta taaggcaaaa ccccattttt acagtagtga
     1621 tataaattgt acctatatca ctatgaaaat tgaatagtaa atatgagaaa gacagttgat
     1681 ttcaaaaaat aagaaattga ttgtcgtttt cttttgcaga aatttaagtg caggcaatta
     1741 tggtagttgt cgtaattgct gtcagctcag tgtttatcac tgatggtgtg aaagatttac
     1801 ggttggagat gttcaataag attattggtg cttaattatt tttattgtag tgctgatatg
     1861 agaatgttat aattggggta taaaacatac aaataaattt gctgaaaaat gaggtatcaa
     1921 aatatggaaa atgaattact ggaaaactta ggaagacttc acacaactga acttggcgtt
     1981 attagaatta aaaagaactt atctttgaat gtagaaaatg taattgaatg gtgcaaagaa
     2041 aaaattagcc tttctaatgc ggaaatcaca agaaaaggaa aaaactggta tataacgatt
     2101 gataattgta ttataacggt aaatgcgtac agttataccg ttatcactgc tcacaaggta
     2161 aaatgataaa tttttctata aaggaattaa acgcatttgc agttattggg caggaagtag
     2221 aactgactaa ttatcaaaaa aagaatattc agattagtac acagttttgg agaaagttta
     2281 acagtagttt gaaaaaagcg tatctttcac aatcaggaaa ttgggtaaaa tatgctttta
     2341 tggaaagaag aaatggaaaa ctttattatt tctgttctat tccccaaaga accattaccc
     2401 cagagggctt tctgtataaa gaaataccgt cttataaata tttggttgtg gagcatattg
     2461 gtgctatgga taaaatatat gaaacttatg gcaagattta tgaagaaatt attcctagta
     2521 catcgtata
//
LOCUS       DAJSKS010000216         2432 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJSKS010000216 DAJSKS010000000
VERSION     DAJSKS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.225"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..602)
                     /locus_tag="ON396_002745"
     CDS             complement(<1..602)
                     /locus_tag="ON396_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR3114781.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNNSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDEL"
     gene            985..1545
                     /locus_tag="ON396_002746"
     CDS             985..1545
                     /locus_tag="ON396_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114782.1"
                     /translation="MIFYEEETVMAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMF
                     EHQRKTNGQPMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMG
                     YNKYGGLDMTIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKE
                     NLLPEVQAIIDSGRVTQWKKIRPFIF"
     gene            1572..>2432
                     /locus_tag="ON396_002747"
     CDS             1572..>2432
                     /locus_tag="ON396_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114783.1"
                     /translation="MNENGEFEESKKYQATSEEEKAAVRELLEEAVPTDFLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVREIEATPEDKKR
                     IESGQIEVIKK"
BASE COUNT          922 a          399 c          513 g          598 t
ORIGIN      
        1 agttcatcca atgtgaaatc ttccgtcatg agtgaaatga gggttgcact aaaaagactc
       61 gtcgattcgt ctcgcatttc tcgagcaagc gtattacatt tgtacgttag attttcaaga
      121 aaatgttgct ttgtacgaat caattgctta ataagttgat agcgagtacg tgttaaacgc
      181 tgtaaggcca tgtatttctc ttctttgata ggggaagttg tgaatcgttg aatacgcaag
      241 aaatcagcga ttcttaatgc atcgtttcga tcagttttat tctcatcaaa catgcgacta
      301 aattgtttca cccgaaaagg attttcaatc gttacaagtg tgttgagctt ctttagtttc
      361 tcatcttcat gaaaaaacat ggaaggatgg aagctgtaca tggacgttga ctccatgcca
      421 atcacaattt gatcaaagtg gtagctgtta ttgaattcaa gaattgtttc tcgtatgaat
      481 gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa ttgattgtcg
      541 tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac aaataatttc
      601 atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac tgtgggattt
      661 cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat aaaagagcta
      721 gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca gctaatgagt
      781 tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac gctacaggag
      841 gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga gaaatcacac
      901 aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa tctagaaaca
      961 ttttgaataa aaactattag acaaatgata ttttacgagg aggaaacggt catggcagaa
     1021 gaaaaagaac cactagacaa tagtcgtttg ggaaaatcca aaagaaaatt agtacgattg
     1081 caaaatgaat taaacgaaca aattgaaaag atgtttgaac accagagaaa gaccaacggt
     1141 caaccaatga acgataagag aaatgggcat tcttggttta ggcaacaaga gcgaatcgaa
     1201 aataaagtgc atagtctgag agaagagatc aagcaacaag aaaaacaagt agaaaaactt
     1261 gaaagacaag aagagataaa ggaaatgggc tataacaagt acggcggact ggatatgacg
     1321 atcgagaata ttccaagaat caaagaagaa atcgaacgct ttgaaaaagg tgaatcgact
     1381 ttttcagccg ctacgatccg aaaataccaa cggaaattgg aaacattaga acagctgaaa
     1441 gaaagatcgg aaaaaggaaa agaaaacctt cttcctgaag tacaagcaat cattgacagc
     1501 ggacgagtga cgcaatggaa aaaaatccga ccatttattt tctgaaaggc tatcgaaaag
     1561 tcgctttaga actgaatgaa aacggggaat ttgaggaatc taaaaaatac caagccacgt
     1621 cggaagagga aaaagcagct gttcgtgaat tacttgaaga ggctgtacca actgattttt
     1681 tgaaggagaa agaaaacgat catttttatc gagtagaatt caacgaaaat gatccagaaa
     1741 aaggattgaa aagttatggt ggggagatcg taacattcga attgatcgat caactcaaaa
     1801 cgttggataa ccaatacgcc gcagaagcag gttattataa attctatttt gaagaagtga
     1861 agaacggaga agtagtcgat cagatccgaa tggatctagg ggatggcgtc aacagtaatc
     1921 aacccatcta tttagagtta gcaaaattgt gcgaacaagt acctgagaag gaacaaaaac
     1981 aatcggtcaa aaatgaacct gtttcgaaag aacaaatgca gtcgaaagaa acaaaaacaa
     2041 aacaaaaggg ctatcaaaaa cgaacggcag aagagattaa acaagaagtt aaatcccttt
     2101 cgcaacaggc actggatgcg gtgaaaaagc atactcattc tcctgaagac gtcaaagaac
     2161 ttttgaactt tatgtctcgt tttccagaac gatcctttag aaatcaatta ttgattgagg
     2221 aacaatttcc aggtgcaacg gcttgtttag gtcgtgccgc gttgaaaaaa gaaggaatct
     2281 atatcaaaaa aggggaacaa gggaacaaga tctttgtcag aaaaacggtc aaaggatttt
     2341 atgagaaagg acgtggcttt gttcgagaaa tagaagctac accagaagat aaaaaacgaa
     2401 tcgaaagtgg tcaaatcgaa gtgatcaaaa aa
//
LOCUS       DAJSKS010000217         2398 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJSKS010000217 DAJSKS010000000
VERSION     DAJSKS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.226"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            207..2069
                     /locus_tag="ON396_002748"
     CDS             207..2069
                     /locus_tag="ON396_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3114784.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="ON396_002749"
     CDS             2069..>2398
                     /locus_tag="ON396_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3114785.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAJSKS010000218         2371 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJSKS010000218 DAJSKS010000000
VERSION     DAJSKS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.227"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            45..>2371
                     /gene="esp"
                     /locus_tag="ON396_002750"
     CDS             45..>2371
                     /gene="esp"
                     /locus_tag="ON396_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HCR3114786.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          861 a          372 c          484 g          654 t
ORIGIN      
        1 tattcttact aggaaagtaa ttattaaggg agttgatttg aataatggtt agcaagaata
       61 ataagagagt atttctagaa aaaacaaaga aacgagtatt gaagtacagt atcaaaaaat
      121 taagtgttgg ggttgcatct gttttgattg gtgttggtct tgtcttggga acaactgaac
      181 tagttaaagc acaagatgaa atttctccaa gcagcccact tgaaacagcg ataagctccg
      241 tacaagtagg tgacaaagta gcttcgggga atacttttca ggagaatcct ggttatacga
      301 aaaattataa cttttcggat ttacaattta gtcctcaaga actaactgga gacacactaa
      361 aaggcaatac aattggattt gaagtttacg gaaaacataa tattgcagca tcaactaaaa
      421 actgggaaat ccgtcttcaa ttagatgaac gattagctaa atatgtagaa aaaattcagg
      481 tcgatccaaa gaaaggaatt ggatcttcta gacggacctt tgtaagaatt aatgattcgc
      541 tgggtagacc tacaaatatt tggaaggtca attatattcg tgcaagtgat ggattatttg
      601 ctggggcaga aacaaccgat acgcaaaccg ctcctaatgg ggtaattacg tttgaaaaga
      661 gcctagatga aatttttaaa gagataggca tagacaatct caagaccgat cgtttaatgt
      721 atcgaatcta cttggtaagt catcaagacg atgataaaat tgtaccgggt attgatagta
      781 cgggctactt tttaacagat tcagatgatt tctataatag cttagatgta tctgaaaata
      841 atcctgacca gtttaaacat ggatcagtaa gtgcaaaata tgagaaacct aatactcaaa
      901 caaaagatgg ttcaggatcc acaggtgcta atggagctat aatcttagat cataagttaa
      961 cgaaaaatta taatttctca tattcatctt ccgcaaaggg cacgccttgg tatgctaatt
     1021 acaaaattga tgaacgatta gttccatatg tagcgggtat acagatgcac atggttcagg
     1081 ctgacaaagt aacatatgat gtttcgtttg aatccggaac aaaagtagct gatttagcaa
     1141 tcgagcgacg ggaaggtcat gaaaattatg gcataggttc aataactaat aatgatttaa
     1201 ctaaacttat cgattttgct aatgcaagtc cacgtccagt cgttattcga tatgttttac
     1261 aattgactaa gccattagat gaaatcttag aagatatgaa agcaacagca caagttgaag
     1321 aaaataaacc atttggtgaa gatttcatct ttgattcttg gttgtcggat acgaataaaa
     1381 aattaatcca gaacacttat ggaacaggtt attattattt gcaagatatt gatggtgatg
     1441 gaaaccctga cgataaagaa gagagcggag acacgaatcc atatatcggg aaacctgaat
     1501 tagaagaagt atatgatgtt gacacaacag ttaaggggaa agtattcatc cacgagttag
     1561 cgggaacagg tcacaaagcc caacttgttg ataaagaagg tactgtatta gcagaaaaaa
     1621 ctatcgctcc aaatgaaaaa gatggggctc caatttcaga tactgtagaa tttgaattta
     1681 cgggtgtaga ttcaagtaaa ttaatcgcga aagatgaatt aaaaatccaa atcgtttctc
     1741 caggttttga taaaccagaa gaaggttcaa ccgttattaa ggaatcacca aaagcggttg
     1801 ataaacaaac cgtggtagtt ggatttaaac cagatgctaa agaatcaatt cggaataata
     1861 aaaacttacc tgaagatgca gagtattcat ggaaaacaga gcctgatact tctaacgtta
     1921 ctgatagtac gaaaggtatt gtaactgtta agatcggaaa tcgaactttc gacgtggatg
     1981 tagagtttgc tgtaaaagct tctcaagcta tggaaaatga tgcaacatac gtacctataa
     2041 caacaactcc agaaacgaca gttcaaagtg gaaaacctac atttgataaa ccagatgttc
     2101 ctttagctaa agatgccttt tcaatttcag atgtctataa taagtacttc ggtaatgcaa
     2161 gtgttgatgc aaacactggt attgttacat tcactccagc taaaggtgta ggagaatcgg
     2221 agccgattac tggaacaatt cctattaaaa ttgtttacca agatggttct gtaggcacga
     2281 ccgatttagc agtaactgta agtaaagata tttatgaaaa tccaggagaa aacattcctg
     2341 caggctacca caaagtaacc ttcaccgcag g
//
LOCUS       DAJSKS010000219         2358 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJSKS010000219 DAJSKS010000000
VERSION     DAJSKS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.228"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..427)
                     /locus_tag="ON396_002751"
     CDS             complement(<1..427)
                     /locus_tag="ON396_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HCR3114787.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYV"
     gene            complement(444..905)
                     /locus_tag="ON396_002752"
     CDS             complement(444..905)
                     /locus_tag="ON396_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3114788.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(919..2169)
                     /locus_tag="ON396_002753"
     CDS             complement(919..2169)
                     /locus_tag="ON396_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HCR3114789.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
BASE COUNT          724 a          500 c          350 g          784 t
ORIGIN      
        1 atacatactt gttaaacatc cgaactcttg tttctttaac ggcatcttct aaattttcta
       61 attcataaca aggatagata atattcatct catcaaattg tctaattaac tgcagttggt
      121 gatcatcgac atgctcacca tacttagcca atcgaggaac agcaatgacc cttttacctt
      181 tttttacggc cccaattata gcacctgtac ctccatgagt aatcaccatt tctgcttttt
      241 gcatagtttc agtaaactca tccctattta gaaatgcttt ataggaataa tttagtggtt
      301 tataatcact ataaccactc tgtgcaaatg tactttcgat tattactcct ttttcaatta
      361 aatcatcaat agcttttaac aatctatcaa attgaaattt ttgtgaacct aatgttataa
      421 atatcatttt tttctcctat tatttaatat atcccaccca aataaatagc attaggataa
      481 aattgtagca tagtttccca ctgaacataa aattgatctg caaaatgata catcaatttt
      541 cctgtctgag tagggctggt tacttttgca aacgattcaa tataaatcag tttttttccc
      601 atcatttttg ttattaagca gatagggatc atagctaaaa cacctgtaca aatcacgaga
      661 tccggttttt cctttatata tataataatt gatttgaata aattaataag tatagtacac
      721 aaaaaaaact tttcttttct atttacttgt ctcatgtagt acgttttttc gccttttgac
      781 tcagctgaat attctgtctg ttctgttata acaaaactct catacttctc catcagtggt
      841 tttaacatca ttaactgttc ataatgcccc ccagaggaag ccgcaaaaca tatctttttg
      901 ttcatattaa cctctctatc agtcacgctg aaaaatatct ctagtgtaaa ctttttcttt
      961 aatatcatcc aagcaggtat catatcgatt ggcaataata gaatcacaca tcgccttaaa
     1021 ctgctctaaa tcgttgataa ccttacttcc gaaaaaagtc tctccatctt tcagtgcagg
     1081 ctcataaatc accacagtcg cacctttagc ttttacgcgc ttcataactc cctggattga
     1141 actctgacga aaattatcac tgttgctctt catggtcagc cggtatatac cgattaaaac
     1201 tggattttcc ctgttgacat catactgatt atattcagcg tagttgtaat agcccgccat
     1261 ctgaagaaca cgatctgcga tgaaatcctt acgggtacga ttagattcaa cgatagcctc
     1321 aatcaaattt ccaggaacat ctgcatagtt tgccaacaac tgcttggtat cttttggcaa
     1381 gcaataacct ccatatccaa aggatggatt gttgtagtga gttccaatac gaggatcgag
     1441 acaaacacca ttgattatct gctgagtatt cagccccttc atttcagcat aagtgtctaa
     1501 ttcattgaaa tagctgactc gcagtgctaa ataggtattt gcaaacagct ttactgcttc
     1561 ggcttcagtg aatcccataa aaagagtatc cacgttctcc ttaattgcac ttttctgcag
     1621 aagtgttgca aaaacatgag cagcatttaa caagcgctca tcagtcatat cagtaccaac
     1681 aataatacga gatggataca gattatcata taaagccttg ctctcgcgca gaaactctgg
     1741 actaaaaata atgttcttac tccccgttgt ctcacggact gattcagtgt agccaacagg
     1801 aattgtagac ttgatgacca tgatggtatc aggattcacg tccatcacaa cctcaataac
     1861 tgactcaaca gcagaagtat cgaaatgctg agtacgactg tcatagttag tcggtgtagc
     1921 aataacaacg aaatctgcgg atttataagc gctccttgca tccaaagtag cagttaagtc
     1981 aagttctttt tctgtaagat acttttcaat atattcatcc tgaattggag acttccgttt
     2041 attaatcagt tcaatctttt cagggataat gtctaccgcg gttacctggt gatgctgcgc
     2101 caatagtgtg gcgatactca aaccaacata accggtgccg gctactgcga tgttgtactt
     2161 tttcttcaca atatctcctc tttcctctct taagtatgga gaatgttact cctacggcta
     2221 ttcatcatgt caaaataaca cagcttagaa tgttgataaa ataattttcc agaatgtgat
     2281 aaaatctctt tcatttataa accacacatc taattttatc tattctccac aagacatgta
     2341 gagtattctt taataaca
//
LOCUS       DAJSKS010000220         2345 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJSKS010000220 DAJSKS010000000
VERSION     DAJSKS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.229"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            49..2202
                     /locus_tag="ON396_002754"
     CDS             49..2202
                     /locus_tag="ON396_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCR3114790.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          802 a          416 c          526 g          601 t
ORIGIN      
        1 tttttgggta caatgaagca tgaatgtaaa taggcggaag gataggaaat gatgaaagat
       61 tttatgaaaa aaatattaaa tacttcttgg ttagaaaaaa taagaaaagg tgcacaagaa
      121 cctaagaaca aaacgtttcg ccggtcccat gttccttttc gcttaaattt tctttttttc
      181 attatattta cgttatttgt tgcgttgatt gctagattgg gttatttgca gatcgtgaac
      241 ggcgaagaga tggaggcgcg agtgaagtct acttctacga ttacggtcca agaaagtact
      301 cctcgcggaa tgatctacga ctccacagga aaagctttag ttacgaataa agcgaatcag
      361 gccatcacat atacgagagg aacacaagtt acggctaaag atttgcttga aatcgcaacg
      421 aagctgaact cgctgatcga tgttccggta gatgaaaatc tgacagatag agataaaaaa
      481 gatttttggc tggctgatcc caaacatttg aaagaagcca caaaaagact ttcggcaaaa
      541 gaaaagcaat tagggaacag cgaacaatat agtgcgatgg tcaataaagt cacagctgat
      601 gagattaatt taaacgagga gcaattaaaa attgctacca tctttaaacg gatgaatagt
      661 gcatatgaat taaacactgt ctatatcaaa aactctggtg ttactgatca agagttagct
      721 gttgtggcag aacgcgcttc tgaacttcca ggagtttcga ctggcacaga ctggacacga
      781 gaatataatg cagcaagttc actgaaaagt attcttggca gtgtgacgac tgaaaagcaa
      841 ggacttccag cagatgaagc agagaaatac ctagcaaaag gttattctcg gaatgaccgt
      901 gtaggacaaa gttatttgga aaaacaatat gaagatgtcc tgcaaggaac caaaacccaa
      961 tatgaagttt cccttgataa tgaaggaaat gtttcgaatc aaaaagaaat cttctctggg
     1021 gaaaaaggtt ctaatttaat gttatctatg aatgcagaat tccaatcaaa agtagaagaa
     1081 attttgaaaa ggaattatca aacattgatc aacaacggca aagcacagta ttcgcctggt
     1141 gcttatgcag tggctatgaa tcctcagaca ggagaagtgc tagcaatgac tggtttcagt
     1201 catgaacaag gttccaaaga aatcaccgag aatgctttag gcacgattac tagcgccttt
     1261 gatccagggt ctgtagtcaa ggctggtact ttgacagcag gctgggcaag taacgcgatc
     1321 agcggaaatc aagttttgat tgatgagccg attcgtctgc aaggtgcttc agaaaaaagc
     1381 tctgtcttta atcgaagcgg acaagttgct cttgatgcag tcaaagcatc ggaattgtct
     1441 tctaatacgt atatgatcaa agtggcgtta aagatgttag gactagacta tacacctgga
     1501 atgggactac ctagtttaga tgaagaagct aaagcttatc agcaattgag agactctttc
     1561 aaagagtttg gtcttggtac aacgactggg atcgatttgc cgaatgaatc tcctgggatc
     1621 tcacgttctg ttgattatat gaagaagttc aatgcggata atggcaaaga atggtatgct
     1681 ccaggtaact ttaccgattt ggcatttgga cagtttgaca catatacacc aatccaatta
     1741 gctcaatatg cttcaacagt cgctaatggc gggaaacgag tacagccgca cctagtcaaa
     1801 gcgatctatg gcaatgacga aaatggaaac ttgggtgaag tgaagaaaga aattggcaca
     1861 acagtagaaa atacggtcaa tatttctgca gaaaatatgg cgatcctccg tgaaggattc
     1921 caccaagttg tccatggtac cgatccttac acaacagcca aaccattagc cagtgcgaaa
     1981 atggaccttt ccgcaaaaac agggactgca gaaacggttg cggaaggtca tccagatatt
     2041 gcgacagtca atagtaatat cgtggcatac ggaccaacag acaatccgga aattgccatc
     2101 agtgttgtct tgccgaactt attagacgaa caagaccaca taaacttgac gattgctaaa
     2161 gaaataatgg acgcgtatta tgatatgttc atggcaaaat aaaaaagagg tcgtgaaaaa
     2221 gccgcttagt ttcgagtatg aagaaaaatt ttgtgaaaat agcttctcac atttttgctt
     2281 aaatttgact tcataccgag aaactggctt ttgaacaccg tttactcggc aaaaaaggtt
     2341 gtgac
//
LOCUS       DAJSKS010000221         2336 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJSKS010000221 DAJSKS010000000
VERSION     DAJSKS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.230"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            25..261
                     /locus_tag="ON396_002755"
     CDS             25..261
                     /locus_tag="ON396_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HCR3114791.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            280..495
                     /locus_tag="ON396_002756"
     CDS             280..495
                     /locus_tag="ON396_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HCR3114792.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1198..1950)
                     /locus_tag="ON396_002757"
     CDS             complement(1198..1950)
                     /locus_tag="ON396_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR3114793.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1965..>2336)
                     /locus_tag="ON396_002758"
     CDS             complement(1965..>2336)
                     /locus_tag="ON396_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR3114794.1"
                     /translation="NKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDR
                     ELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNP
                     NMFVNYQNDPYQFTTQVILLF"
BASE COUNT          758 a          373 c          393 g          812 t
ORIGIN      
        1 gtaaaaaata ttatttaaag aagcgtgttt aaacatgcaa aatgtaaagg aactaagtat
       61 tgtggaaatg cacaaactgt tggtggaggc aataatgctt ggggaaaatt ggggcaagtt
      121 gtaggtggac ttaccacagg ggcagtaggt ggtgcaggtt taggaacagc aatttgcgga
      181 ccagcttgcg gtgttgttgg agggttatat ggagctgtcg ctggaggtgc tgcagctggc
      241 tgggatgctc gaaaaaaata aaaaaggaga ataagtagta tgcatattaa aaacacaaaa
      301 acaacattta tattatcttc agaagagttg aaaaatattc aaggtgggag tgccgtggga
      361 gttttaggca ctactttttc tggggcaaca gcaggagtaa aactttgttc agccggagga
      421 ccttatgcaa tagctgcttg tggtgtaggt ggagctttat taggtgctgg attcagcatg
      481 tggaccggtg cgtaaacaat taaaaaatat caaacctcaa gattggtttg tgtcgattac
      541 gaacataggt atgttcttta tcttttcaaa agtttggaat ggacagaact ttttactaaa
      601 gcttcttgtg ctagttttta taattatatt aaatttttat cttttattaa agtcagataa
      661 aaattctttg gaaatgtaat ggtagagata tttttacaaa aggacttgca gcgctgtagt
      721 tggtgctgca ggaggaccta tttcggccgt tacaggaggt attggtaatg tacttgtcgg
      781 atgtgcatta aagcctgttc ctgcaagtta aatttataaa aacaggaaag taattatctc
      841 aaatcctatc aagtacaatc ataggattgc acttgacagg acaaattttt taactaaaaa
      901 atgaaaaaca ctttctaaaa attagtagaa tttgccaaat ctttcgcatc ttccaccata
      961 catttaattt taattcccct ttatggtact taataaaaac aagaaaagaa ctattcattt
     1021 ggcccttttt tggttccaga ccatagaaat attcctccag taactaatat tgctgttatg
     1081 cacgtaacta ataacagcta taaaaattgg tacaacttta ataggattca gaaaaatttg
     1141 cactagtgaa ggaatctatt ttattagaaa aatgcaatct ccctaaagaa aaaaccacta
     1201 aacagtgctt tctttcaact tctccttaat tttttgtgct ttccctaaag taaagtttct
     1261 ggccaactct gaatcaaaat atagtgaacg ttttttaaaa tctatttctt taacgttaaa
     1321 gatatttact aaacacgctc gactaattct tgttaacata ggatatttct tttcaagctc
     1381 actaattctt ccataaaatt cgtactgccc atctttagta cacaatgata atctatgtgg
     1441 taattttgaa gcttctacaa aataaatgtc atttatatca aaagtaaatg tttgagaacc
     1501 tatggaaaaa acaaatgctt gattcgtaac ctgttttgtt gcatctatcc tttcttgcgc
     1561 taataacaat aactcaacaa tctcgtttcg atactcatct agtgtttgat cttttgttac
     1621 aaatcctaac gtttcaactc ttctttttat tgttacgggt aacatctcat catgagtagt
     1681 tgtaaaaatg atttttgctt gaacatcata ttttctgatt acttctgcta attctatacc
     1741 gttaacttca tgatttaaat caatatctaa gaaatatatt ccttgctttg gtcggaattg
     1801 tttgaggtat tttttaactt ctaacggact ttgtgttttc aatactatct taaatacttt
     1861 atcgtgaaaa agaatgaaat tatcaataat tcgttctaac tgatttaatt gtataatttg
     1921 atcttcacat aatattattg gataactcat aaatcctcct tccctcaaaa caacaaaata
     1981 acttgagtag taaattgata cggatcgttt tgatagttca caaacatatt gggattcttt
     2041 ttattgattt cctctatact agataaacct aacccttgat gtcccttttt tgttgttatt
     2101 ccttttttca ttagagtatc tatagagagt agtatctctc tacaactatt cgatatcgaa
     2161 atttctatct gtttatcatc tcggtaaata gctagagaca gttctctatc ttgactctct
     2221 atagatgctt caattgcatt atcgagaata attccaagca ccctaataca gtcaaatatt
     2281 ggaacaggaa cttcaataat agtacttgga cattcgaatt tacaacaaat cttatt
//
LOCUS       DAJSKS010000222         2261 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJSKS010000222 DAJSKS010000000
VERSION     DAJSKS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.231"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            278..820
                     /locus_tag="ON396_002759"
     CDS             278..820
                     /locus_tag="ON396_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114795.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..1425
                     /locus_tag="ON396_002760"
     CDS             976..1425
                     /locus_tag="ON396_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HCR3114796.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1455..>2261)
                     /locus_tag="ON396_002761"
     CDS             complement(1455..>2261)
                     /locus_tag="ON396_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HCR3114797.1"
                     /translation="TYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGL
                     QLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDL
                     TYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDR
                     GKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLR
                     LELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          784 a          372 c          429 g          676 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccagatggaa tggcaacact ttttttgaca aaaattataa ggtgttgtga atgctgcggt
     1381 gttatggcta cgattgccct tgacaatgag catcctcgga tctgattttc tccggcagaa
     1441 gcacaatcat tcaattacgc tgcctctcca gaggtatcag atccgcgctc attatcaagg
     1501 attcgcttcg ccaatctctg ttgtcgaatt tggatcggtg tttcatacgc caacgtgcca
     1561 tgtaagcgta gatagttcca ccaatgcaca taatcaaaca attctagacg tagctgtgcc
     1621 agtgtctcaa attggtattg atgcacgaat tctactttga cagatttata ggttgattcc
     1681 acaacggcat tatcatacgg acaacccttc ctactcaatg aacgagtaat tccaaaacca
     1741 ttcagaatct catgaatggt ttggttatca aattcctttc cccggtctgt atgaaaaagc
     1801 ttgacgtctg ttaaagaata cggtatccgt ccaaaggctt cttttaccaa tgaggcatct
     1861 ttcttctcac cacaggaata accaataatt tctcgattaa acaaatcaag tattaagcag
     1921 atataatgcc actttttccc cacgcgaaca taagtaagat ccgtaacgat ggcttccaat
     1981 ggctgttctt gtgtaaaggt ccgatctaat acattcgctg ttttcgcttc attacaagct
     2041 gttcgttgcc ctttaaaatg agcgatcgta taggtagatg tcaatccgcg gcgtttcatg
     2101 attcgaccga ttcggcgccg actgagttga agcccacgct ttgctaaaca cttcttcaat
     2161 tttcgggtac cgtaagcctt tcggtttcga ataaactctt cctgaacgat ctcttctaag
     2221 tcggactcat tttcgatcgg tttcgcttga taatagtagg t
//
LOCUS       DAJSKS010000223         2248 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJSKS010000223 DAJSKS010000000
VERSION     DAJSKS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.232"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            84..1697
                     /locus_tag="ON396_002762"
     CDS             84..1697
                     /locus_tag="ON396_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR3114798.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          705 a          399 c          444 g          700 t
ORIGIN      
        1 aataaacaat aattttccgg cacaattatt tgctgttttt taccataaca tagtacgatc
       61 aacgtagaaa aggagatgtt tttatgaaag ttgttgtcat tggcggtgta gctggcggtc
      121 cttcatttgc cactcgtttc cgtcgattga atgaagcgca cgaaatcatt atctatgaac
      181 gcggagaaaa tatttcttat gcaagttgtg ctttgcctta ttatttagga ggtgtgatca
      241 ctgaccgtga ctcgctgatc gaacgtacac cagaaatatt gaaaacaaaa aacaacatcg
      301 atgtctttac taaacacgaa gtaacagcaa tcgatccttc tactaagcga ttaacagtta
      361 aagacctatc cacaaatgaa gaaataaaaa cagattacga taagttgatc atctcttctg
      421 gtgctagacc agattatccg gatattcccg gagtttttga agcagaaaac ggctttgtcc
      481 tccgtagtgt gacggatgcg gatcgaatca aatcgttcct tgaagaaaaa aatccacaac
      541 atgtcgtcat tcttggtgca ggtgttatgg gtctggaatt agctgagaat ttcaagcatc
      601 gcggcttaga cgtgacttta atcgatcaat tgccacaagt cgctttccct tatgatccag
      661 aaattgctaa tttagtttat gacaaattgc tgaaagaagg attagccgtt catttagaaa
      721 caagagttac tgagatccgt gataaaggtc gagaaatagt attatcagat ggctccgtcc
      781 tttctgctga tatgctaatt tttgctgttg gtgtttctcc gaagaatgaa gtggtgaaag
      841 caaccggcat acaattatca gatacaggtc agatcattgt tgatgaccag ttacaaacca
      901 atctgccgga catctatgcg attggcgata ttatcgaaac gactagtgta gtgactggtc
      961 agccgatcca aagtatgctt tccagtgcgg ccaatcgtca aggacatatg ctggcagata
     1021 ttttaaatgg tacgcctatg cgctatcgcg gatatattgg tgcaggtgtc gcaaaaatct
     1081 ttgatcatac agcaagttat gcgggaatga cagaacatga attaaaagca tcaggcataa
     1141 caaattataa aactgttttt atcactccct ttgaccatgc ctatttctat ccagaagcta
     1201 caagattaaa tctaaagctg atttttgatg cagatagcgg tcgtatttta ggtggacaag
     1261 catttggaga aaaaggtgtc gataaacgga tgggagaact ttctgtagca atcaccggaa
     1321 acttgacagt ctttgatttg cctgatttgg agttgcctta ctctccaccg tattctacta
     1381 cccgtgaccc gttgaatata gctggttatg tcgcaatcaa tcaaatgacg aatatcgtag
     1441 aaacgatcaa agcaagtgat atacccgaaa acgatttgaa agaagcgttc tttttagata
     1501 tacgtgaacc taataaagca ccttctggca gtatctcggc aacaaaaaat attccaatga
     1561 atgagttacg agatcgaatc aatgaaatcc caaaagataa aaaagtttac attactttca
     1621 gaagaggatt gaatacttat acttctgccc gaatcttggc aggtttgggt atcaaagcga
     1681 ttttgattga agaataaaaa aacgaccgcg gtgggtagcc gcggtctagg agttattttt
     1741 acttgaggag taaaaatgaa aaagtatata gggttgttgg ttggtatgta tatagaatag
     1801 aagataaatg tgaagaaatt gtgcattatt tcttcacatt tattaggtta tagtttaaga
     1861 aagtttaaac attgcgcaat ttaattattt tagctgtttc ttttcataaa aaaacatgtt
     1921 gtgactctga gacaaatcat agtccatagt gatgtctcta gagtaactac gcctaatatt
     1981 acgaggcatc atattaatct atcagcacta gtacctaaac tagcatccct ttcgaatatt
     2041 ttttcttata taataaagtt tgctttcact cctctaatct caccaaatcg actggcttgc
     2101 gttgtgtgat actaggaact gattggttta gaacgacttt tctattgtaa tcatattttt
     2161 cgccatcctc gttgtcttct aatccttcaa tgaatgatcc ctaatgtgtg tattaaataa
     2221 tttaattttt ttgttcctat ttatttaa
//
LOCUS       DAJSKS010000224         2169 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJSKS010000224 DAJSKS010000000
VERSION     DAJSKS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.233"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(132..1058)
                     /gene="cysK"
                     /locus_tag="ON396_002763"
     CDS             complement(132..1058)
                     /gene="cysK"
                     /locus_tag="ON396_002763"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR3114799.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(1198..2085)
                     /locus_tag="ON396_002764"
     CDS             complement(1198..2085)
                     /locus_tag="ON396_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR3114800.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          601 a          485 c          362 g          721 t
ORIGIN      
        1 aagacgtaaa acagtcgcgt tcactattct acgtctctat tcaaataatt tttctcatca
       61 taaatttttg tgtgttcttt tcatctttaa aagtttttta cataaaccta tctggagaaa
      121 aaccaagtgc cttatatctc ttggtaaagg gctgtagata aataacgttc accattgtcg
      181 ggaaccacag ctaatacctt cttgcctttt cctaattctt ttgcgacttt tagcgcggca
      241 gcaattgcag cgccagatga gatgcctact aaaatccctt cttttcgccc aacttctcta
      301 gctgtttcca tcgcttcgtc tccactaata gaaaggactt tatcatacac agaagtatcc
      361 agtgttttag gaacaaaacc tgtaccgatt ccttggattt tgtgaggtcc cgcttctttc
      421 ccttctaaaa tagcagattc tgctggttct accccgatta attcgatttt cggatacaca
      481 cgttttaatt ctcggccagc gccagtgatc gttcctcctg taccgatacc tgcaacaaac
      541 gcgtccaagc catttactcc aaatgcttga tgtatctcag gaccagtcgt cttctcatgc
      601 acaagaggat ttgcttcatt ttcaaattgt aatggtaaaa agtagccgtt ttcttttgcc
      661 aaacgttctg cttctctgat tgatccagaa ataccatccg caccaggagt caaaatcaac
      721 tctgcgccgt accctttcat caataaacgt ctttcgatac tcatcgtctc aggcatcacg
      781 atgattactt tatatccttt tgcaactcct accatcgaca atccgattcc tgtatttcca
      841 gaagtaggtt caatgatagt atcacctggt ttcagtagac catcgtgttc tgctttttct
      901 atcatactta aagcaatacg atcttttaca cttcctcctg gattaaaaaa ctcaagcttt
      961 acaaaaacat ctgctgaatc ttctggtacg attttattta atttgacgat tggcgttttg
     1021 ccaatcaatt cggtgactga gttatagata gctgtcatct tcaattcctc cttgtctttg
     1081 ctcttcttac ttatcataat acaatctgtt catttatggc gagtgattcg tctttgacta
     1141 gcaaaaaaaa gacaaagata taccatatat ggtaatttct ttgccttttt atctaaatca
     1201 aacgtttagt tcttgaccaa ctaataaaga ataactggac atagacatgc catttttcgc
     1261 cattagatca tcaacagaca tgccaaattt ttgtgcaata tcccatagcg tatcacctga
     1321 cacgactgta tatgtctgac cattatttgt atttacatct gttgttgaag atgatgacgt
     1381 tgaagtcgtc gtagtagtcg ttgttgttgt acttgtggtt gttgtagccg aacctgttga
     1441 tggcgtatca aaacgggtta aattataagt attaatcaat gagttcaatt tggccccata
     1501 atttggatcg gtagcataac gacctgtcaa gtacgctgtt gcatcataaa aacttgttgt
     1561 attgcttttc cacacgccag cataatgata atcgcccgtg gaaaaactgg ttgaacgaag
     1621 aacattcgca tggtcttgga aggattccca ataagaagaa tattttcgga atggctcatt
     1681 catcgttacc cattgtccgt ttacatattc tttggttgcc atataaacga cctgtccgtt
     1741 atagcttcct ttcacaccga atagattata atttggcgca ctagctaatc cggaagtgcc
     1801 atatgcactt tccaacaacg cttgagcgat catgactgaa gcatacaagt cattacttgc
     1861 agcaacttct gacgcagacc agccgatttg atcgataaac gcttgaggat cagcgactgt
     1921 ttgagatcct tgttcatctg catgaacatg ttgcggtacc gctgtaacca tcatcggccc
     1981 aactaaaaga ctcgttccaa ttaaaggagc aatcgctttt ttattgatca ccatttttct
     2041 tttaggtcta tggtgccggg cactgcgggt cttcagctct tgcattcaaa ttcctcctca
     2101 aaataaaaaa acttaataaa aagttctctt cttaaaacaa acttaattaa ttaaattata
     2161 cttttaatt
//
LOCUS       DAJSKS010000225         2123 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJSKS010000225 DAJSKS010000000
VERSION     DAJSKS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.234"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..1525
                     /locus_tag="ON396_002765"
     CDS             <1..1525
                     /locus_tag="ON396_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR3114801.1"
                     /translation="NRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAGLNN
                     LFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVFTLG
                     LLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVINVS
                     MLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVGELE
                     KETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIMSIF
                     AIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMIIWVG
                     SDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNIILV
                     NWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVASYL
                     SYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLVKR"
     gene            1703..1924
                     /locus_tag="ON396_002766"
     CDS             1703..1924
                     /locus_tag="ON396_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HCR3114802.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT          709 a          226 c          404 g          784 t
ORIGIN      
        1 taatcgtaaa caaaatgcta aaaggaatat aattactgga acaattttta agttatatca
       61 gattatcatt ccctttttta tgcgtacact tatgatttat cagatgggaa tggagtatgc
      121 tggattgaac aacctgttca catctatatt tcaaatgtta aatttagcgg aattgggaat
      181 aggtgctgca ctaactgtaa gtatgtataa acctatagca gaagacaata gaaaagagat
      241 ttgtgcttta ctgaacgcat ataaaaagta cttttcaatt attggatgga tagtgtttac
      301 attaggattg ttatgcattc cttttttgag atttttagtt aaaggttcga ttcctggaaa
      361 tttaaatatg acagttttgt atttaatgta tttgataaat actgttttgt cgtattggtt
      421 attttcatat agaaagagtc taatatatgc acatcaaaga aatgatgtaa ttaacgtgtc
      481 tatgctaatt acttttacat tccaatttgt aatgcaggca tatgttctga ttttcttaaa
      541 aaattactat ttgtatttat tggcttctat tttgggacaa atagttctta acttaatgtg
      601 tgctgttgtt tctcaaaaga tgtatcctga ttatactcct gttggtgagt tagagaaaga
      661 aactattgac gatattataa aaaaagtaaa aggattagtt acaaacaagg taggcggtac
      721 aatattacga tcttcagatt caattattat ttcatctttc ttaggtcttt cagtacttgc
      781 gatttatcag aattattact ttattatttc tgccataatg agtatatttg caataattta
      841 tgaatcatgt atagcaggta taggtaacag tcttattgta gaaagtcaag aaaaaaatta
      901 tatagatttt tgtgatatta caacaattat aggttggttt ctggcaatta gttgtgcatg
      961 ctttattaca ttatttcagc cgttcatgat tatctgggtg ggaagtgata atcttatgag
     1021 tatttcattt gttatattta tgatagttca ttttttctta tatgaaattg atcagttaat
     1081 tgggttatac aaagatgctg ctggaatatg gtacgcagac aggtttagac cattaattac
     1141 agcaatgata aacattgtat taaatatcat cctcgttaat tggttcggat tatatggagt
     1201 tttggcggca accattgttt cagtattgtt ggttgattta ccctggttat tatataatgt
     1261 tttccaaaat atatttaaat ttgaatcggc aggtaaatat gtttggaagc tcataagttt
     1321 gtgtgtaata gcttttgttg cttcttattt aagttatttt gcttgttcgt atgttaatat
     1381 cggaaatttc aaagtgataa gtttagtgat aaatttagtt atatcggttg tcatatcgat
     1441 tttagttatt cttctgttta atatccgtaa taagaagttt aagtatattt tgaaagttct
     1501 tacgtcggta ttggtaaaaa gatgagaaga gacatcagaa tgatatataa agtaactaca
     1561 atttttaggc tgccgaatag gcgaacgatg tatagttttt gttccaacga agactcttgt
     1621 tgaaattgga gatgatatga aggagttaca attttgactc atgtttatga ttgatcggtt
     1681 ttaaaagtag tatggagaag ctttgggatc atcggagagg atcaaaatag gtaataatgt
     1741 ttttatagga atgaagtcta gaatactaaa gggtgtacat ataggaaata atgtagttat
     1801 aggtgcaaat agtttagtaa acaaggacgt atcagacgat tgtgttgttg caggaaatct
     1861 agcaaaagta attatgtccc tagatgaata ttataaaaag ataaacaata tgaagaatcc
     1921 gtaaaacttg tgaaactata ccgagaataa tatgggaaaa atattgatga aaaagcatta
     1981 tatgagtttt tagggctgtt tacagatgga gatgacaatt tgtcgaagag ttgggagcaa
     2041 tgcaaaattt agtaggggat tataaacaga ctgacaaggt atttaaagaa catgcacaag
     2101 tatttcataa tattgaagaa ttt
//
LOCUS       DAJSKS010000226         2109 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJSKS010000226 DAJSKS010000000
VERSION     DAJSKS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.235"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..528)
                     /locus_tag="ON396_002767"
     CDS             complement(<1..528)
                     /locus_tag="ON396_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCR3114803.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFY"
     gene            complement(525..1367)
                     /locus_tag="ON396_002768"
     CDS             complement(525..1367)
                     /locus_tag="ON396_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR3114804.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(1369..2088)
                     /locus_tag="ON396_002769"
     CDS             complement(1369..2088)
                     /locus_tag="ON396_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR3114805.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT          717 a          410 c          242 g          740 t
ORIGIN      
        1 atagaatttt ttattatcag gatctttttc cgctaactgt ttttcaatat ttttagcgta
       61 aataataccg ttttctaaat ttaaccaagc atgaggatct tccttccctt tctcactctg
      121 accctctaag taaataacat ctatgccatc acttgctgca aaatagtctt tgttttcctc
      181 tttgttcgca tttttaacta attttgtaaa ccaagcattt cctccagttt ccaagttaac
      241 accgttataa aaaatcaaat ctgcttttga agttttttga acatcttcag gcaaaggttc
      301 atattcgtgg ggatctttcc caataggtac gatactgtgt aaatcaattt tatcttttgc
      361 tatattttcg gtaatatctg ctaaaatcga attagtaact actactttta atttttcgtt
      421 cgaagccact ttatctttgt ctttactatt tattgaacat gccccgaata taaaaagagc
      481 aatcaaaatc cccagacttc ccagtactgc tttccatttt ctcatcataa cgatctctcc
      541 ttctttctta aaaatttttg ttttggtgag attagaaaac taattacaaa taagaatgcc
      601 gctgttaaaa caatacttga tcctgctgct atgttaaaac tataaccaat aaacaatcca
      661 agcaatgatg aacttgctcc gactgttgca gaaataaata tcatagtttt caagctattt
      721 gcatataaat atgctgtagc tgctggtgta attaacattg caacaattaa tatagttccc
      781 acactttgca tagctgttac tgctactaaa gttaatagaa ccatcaatag ataatgataa
      841 aaactgacat tcatccctat tgcttttgcc attaatggat caaaagaagt gattaataat
      901 tctttaaaaa atagaactac tataaccaac actaaaaccg aaacgccaat tgtaatccac
      961 ttatcaatat cttgtacagc taaaatattt ccaaataaaa tatgaaataa atccgttgag
     1021 ctatttgcaa ctcctataag gataactcct agtgctaaaa aagaactgaa agtaatacca
     1081 attgctgtat ctccttttat tacactattg ctttttataa aagtaataat gatagaagct
     1141 aatagaccaa atacaatcgc accaataaaa aaattaatac ctaaaatata agaaattgct
     1201 actcctggca aaacagcatg agaaatagca tcacccataa gagacattcc tcttaaaata
     1261 ataaaacaac ctactgttcc agaaacaatc cctataacta cagatgtaat tagagcattc
     1321 tgtaaaaagt gaaagtcata cagaccatta ataaaatttt gaagcatatt attccccctc
     1381 tccaataaat atagtatctc cataggcttt ttttaaattt tcttcattaa atacagattc
     1441 tacacttccg tgagcaatta gatttcgatt caaaagaata atattatcaa aatacttctt
     1501 taccttgctt aaatcatgat gcacaattag cactgttttt ccctgatgtt tcaatttttt
     1561 taacgtatcc ataattattc gttcactaac taagtctatc cctacaaatg gttcatctaa
     1621 aaatatgaaa tccgcatttt gagcaagaca tcttgctaaa agtacacgtt gaaattgtcc
     1681 tccagataat tctcctattt gatgattaga atattttccc atatcgacct tttccaaagc
     1741 ctttgaaact ctttgccatt ctgcattttt aattctttga aaaactttca ttccggcata
     1801 tgttcccata gatacacatt cttttacttt tattggaaaa gtaaaatcaa tatcagcttt
     1861 ttgctctaca tatgcaatct tcaccagttt tttattgata tcttcattat taatggagac
     1921 ctctccctga tgagggataa tatttaacat tgctttaagc aatgtggatt ttccagcacc
     1981 atttggacca ataatacctg ttaaagcacc ttcttgtatt tttaaagaaa tctcgttcaa
     2041 tgctaaaaaa tcgttataac aaacattaag ctttttcact tctaacataa ctttctcctt
     2101 ctaaatgat
//
LOCUS       DAJSKS010000227         2106 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJSKS010000227 DAJSKS010000000
VERSION     DAJSKS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.236"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..525
                     /locus_tag="ON396_002770"
     CDS             <1..525
                     /locus_tag="ON396_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114806.1"
                     /translation="LEKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTI
                     SNEINQYREREPEIQPAEVKQEHKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRT
                     LHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKN
                     EIYYEQQLSQKERV"
     gene            586..2055
                     /locus_tag="ON396_002771"
     CDS             586..2055
                     /locus_tag="ON396_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR3114807.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
BASE COUNT          804 a          327 c          457 g          518 t
ORIGIN      
        1 ttggagaaag ctattcctta tattgcagca tggacgaaga atggtcagac gttggatgat
       61 aaggaagctg cggaacgtgg gaaaatcatg aatgatgtca gccgtgtcgc aaatgaattc
      121 attcaaacca tttctaatga aatcaatcaa tacagagaaa gagaaccaga gatccaacca
      181 gcagaagtga agcaggagca taaaaatact gtagaaacgg cagaacaaaa gaatattgtt
      241 cctgttggtt cactttggat cgacaagaaa gacgggaaag taatggaaga agatacgggt
      301 cgaacgttac atttgaagga tgcggcgttt attgaagatc cacagcataa tcaagtaatt
      361 ttaaaaggaa aaagttatgg aaaagagatc caaacggcgt taccttcaga agaatttgcg
      421 ctaggaaatt cattccgatt ttcacttgag acagtgtcag aagtggcaaa acgaaaaaat
      481 gaaatctatt atgaacaaca attgtcacaa aaagaacgtg tataaaacaa gtaaaaaaga
      541 ctatctgaaa aacggatagt cttttttctt ttgggaggtt ttaaaatgac cgagaaaaaa
      601 atgagtctta ttgatcgatg taaacagatc gatatagtgg attttgctcg aaacaatgga
      661 atggctgtag tcaataaagg acgagactat cgcttagaag accatgattc atttgttttt
      721 gatcgaagaa aacaacgttt ttattggaac agtcaaaata ttagtggcga cattattgaa
      781 ttagctaaac tttttttcat agacaaagag atccaagacc ctaagcaaca atttaaagca
      841 gccactgatt ttattctaaa gaatgaagac aaaacagaac gagtggaaaa tcttcatttt
      901 gagacagaaa agtacaaaga tcaccccgtt gattatcaac cactcacaga aaaaggaagg
      961 aattatctga aggaagaacg aaaactacca gagtggctga ttgattatgc agaaaaagaa
     1021 gggttgattg ctgaactaaa gccgaaacat gaacggcaaa attttttagt tggagacgac
     1081 cgtttagatc atgcggttgc ttttttatgg aaagatccac agacaggaga gacagtaggg
     1141 gcaagttatc aaggaacgat cgtcgatttt aatcgtttcg gtaaacgggg aacgtataaa
     1201 cacattgaca agaatcctac gccaaatcac ggatttaatt taaaaatagg cgatccgaag
     1261 catttaaaat tctttgaaag tagtattgat ctgttaagtt acgctgcatt gaatcgggag
     1321 aaactacaag atgcctggtt agtttcgatg gacgggttaa aacatcatgt aatcagtcat
     1381 tacgttgaag agtcgatttc tgaattaaga cgaaaacaaa catttcctca atcgattgaa
     1441 atatgtgtgg acaatgatcg tgcaggacat attttttatg aaaaagaaca gatgaaggga
     1501 atcgttgatc cttttaccaa taagaaaatc cgttgtgagc gaggaattcc aaatgattgg
     1561 caagtaccta aagaatacaa agctacttat gaagcagtcg caaaagaaat gagtgttgaa
     1621 cctgaagcaa ttatggccat acacaaaact gaaactaatt tacagctgac aaatcaattg
     1681 gtgtctgctc atgatgttca atctacgttt ggaaagatgt tggcgaaggg cgaaccagta
     1741 gaaacaattg atttgaaaga agcatgtacc acggtcgcga aggaactgaa agtatgtgaa
     1801 cgcgcagatg gaacatacaa ctttgatcgt ttttatagta gaaaagccaa cataaaagac
     1861 gttaatgcag gaatccttct ttcctataaa gcagaacaat actacaaagg ctataaaaag
     1921 catgaacatg aatttgtacc tgaagtgaaa aaggattgga atgatcaatt gaagcatgag
     1981 atccagcaac aagaaatcag aaagcaaaaa cgtgcgatgt tgtttcaaca aggcagacaa
     2041 caagaaaggg agtgaagtcg tcactatgaa aaccgtcatt ttagcagaaa aaccgtcaca
     2101 agcgaa
//
LOCUS       DAJSKS010000228         2097 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJSKS010000228 DAJSKS010000000
VERSION     DAJSKS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.237"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..525
                     /locus_tag="ON396_002772"
     CDS             <1..525
                     /locus_tag="ON396_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR3114808.1"
                     /translation="SEKAIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTI
                     SNEINQYREREPEIQPAEVKQEHKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRT
                     LHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKN
                     EIYYEQQLSQKERV"
     gene            586..2046
                     /locus_tag="ON396_002773"
     CDS             586..2046
                     /locus_tag="ON396_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR3114809.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
BASE COUNT          795 a          329 c          453 g          520 t
ORIGIN      
        1 tcggagaaag ctattcctta tattgcagca tggacgaaga atggtcagac gttggatgat
       61 aaggaagcgg cggaacgtgg gaaaatcatg aatgatgtca gccgtgtcgc aaatgaattc
      121 attcaaacca tttctaatga aatcaatcaa tacagagaaa gagaaccaga gatccaacca
      181 gcagaagtga agcaggagca taaaaatact gtagaaacgg cagagaataa gaatattgtg
      241 cccgttggtt cactttggat cgacaagaaa gacgggaaag taatggaaga agatacgggt
      301 cgaacgttac atttgaagga tgcggcgttt attgaagatc cgcagcataa tcaagtgatt
      361 ttaaaaggaa aaagttatgg aaaagagatt caaacggcgt taccttcaga agaatttgcg
      421 ctaggaaatc cattccgatt ttcacttgaa acagtgtcag aagtggccaa acgaaaaaat
      481 gaaatctatt atgaacaaca attgtcacaa aaagaacgcg tataaaacaa gtaaaaaaga
      541 ctatctgaaa aacggatagt cttttttctt ttgggaggtt tgaaaatgac cgaaaaaaaa
      601 atgagtctta ttgatcgatg taaacagatc gatatagtgg attttgctcg aaacaatgga
      661 atggctgtag tcaataaagg acgagactat cgcttagaag accatgattc atttgttttt
      721 gatcgaagaa aacaacgttt ttattggaac agtcaaaata ttagtggcga cattattgaa
      781 ttagctaaac tttttttcat agacaaagag atccaagact ctaagcaaca atttaaagca
      841 gccactgatt ttattctaaa gaatgaagac aaaacagaac gagtggaaaa tcttcatttt
      901 gaaacagaaa agtacaaaga tcaccccgtt gattatcaac cactcacaga aaaaggaagg
      961 aattatctga aggaagaacg aaaactacca gagtggctga ttgattatgc agaaaaagaa
     1021 gggttgattg ccgaactaaa gccaaaatat gaacggcaaa atttcttagt tcgagacgat
     1081 cgtttagatc atgcggtcgc ctttttatgg aaagatccac agacaaaaga gacagtaggg
     1141 gcgagttatc aaggaacgtt cattgattat gaacgttttg gtgagagagg cacatataag
     1201 catatagata aaaattcgac agcgaatcat ggctttaacc taaagatcgg tgaccctaag
     1261 cagttaaaat tttttgagag tagtattgat ttacttagct atgctgcact gaatcgtgat
     1321 cagttaaacg atacgtggtt agtctctatg gaagggttaa aacaccatgt gatcagtcat
     1381 tattttggtg aggcagtatc tgaattgaga aagaaacaag cctttcctca atccatcgag
     1441 atttgtgtgg ataatgaccg tgcaggacat attttctacg aaaaagaaca attaatgggc
     1501 gcagtcgatc catttacgaa tcagaaagta cgttgtgaac gaggaatcgc taatgattgg
     1561 caagtgccaa aagaatacaa aatcatttat gaagaagtcg caaaagaaga aaaggtcaca
     1621 ccagaagcta ttatggccat acacaagaca gaaaacaatc tgcaattaac gaaccaactg
     1681 gtttccgctc acaaagtaaa tgcgtttttt ggccagcagc tttctgttaa cgattcaata
     1741 gaagccatta atttgaaaga tatttgtaga gaagtcgcaa aagaattaaa agtttgtgaa
     1801 cgcgtggatg gtacgtacga ttttgatcgt ttttatcaag aaaaagggga tattaatgcg
     1861 cagattcttt tctcttataa agcagaacaa tactacaaag gctataaaaa tcatgaacat
     1921 gaatttatac ctgaagtaaa aaaggactgg aacgatcaat tgaagcatga gatccagcaa
     1981 caagaaatca gaaagcaaaa acgtgccatg ttgtttcaac aaggcagaca acaagaaagg
     2041 gagtgaaatc gtcatgatga aaaccgttat tttagcagaa aagccgtcac aagcgaa
//
LOCUS       DAJSKS010000229         2085 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJSKS010000229 DAJSKS010000000
VERSION     DAJSKS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.238"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..1280
                     /locus_tag="ON396_002774"
     CDS             <1..1280
                     /locus_tag="ON396_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3114810.1"
                     /translation="LSIKDATDRASKETFANITNSFSMEINRQVNPGTPRGGGNVKGE
                     DIKKISQTDSIDSYVKRINSVADLVDYDIIETQETLANQSPERAKNFKRTVMLTGVND
                     STKETKFVSEAYKLVEGKHLENEDKNKILMHKDLAEKNNLKVGDKIKIKSNLFDADNE
                     KGADETVEVEIKGLFDGHNSGVVSAAQELYENTLITDIHTAAKVYGNTEDTAVYQDAT
                     FFVKGDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSISNKLFFGSLI
                     FAGVVVSLLLFLWMNARKKEIAVLLSLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQ
                     YTADKLGNNILNKVTGDIAKQIAKQSASSQLGGGAEAEGFNKTLSSLDINVLPKFMIY
                     VVIFMSLVLLVSLIISSFNILRRNPKELLIDNN"
     gene            complement(1566..>2085)
                     /locus_tag="ON396_002775"
     CDS             complement(1566..>2085)
                     /locus_tag="ON396_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3114811.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIV
                     MFLWLFRMGVIP"
BASE COUNT          755 a          308 c          378 g          644 t
ORIGIN      
        1 gtttatctat taaagatgct acggacagag cttcaaaaga aacatttgct aatataacaa
       61 atagtttttc tatggagata aatagacaag taaatccagg aacacctaga ggtgggggaa
      121 atgtaaaagg tgaagatatt aaaaagatat ctcaaacaga tagtatagac tcctatgtaa
      181 aaagaataaa cagtgttgca gatttagttg attatgatat tattgaaact caagagactc
      241 ttgcgaatca atcacctgaa agagcaaaga attttaaaag aacagttatg ttaactgggg
      301 ttaacgactc aacaaaagaa acaaaatttg tatcagaagc atataaatta gtagaaggaa
      361 agcatttaga aaatgaagat aaaaataaaa tcttaatgca taaagattta gctgaaaaaa
      421 ataaccttaa agttggagat aagataaaaa taaaatctaa tttatttgat gctgataatg
      481 agaaaggtgc agatgaaaca gtagaagtag aaattaaagg tctattcgat gggcataata
      541 gcggtgtagt aagtgcagca caagaactat acgaaaatac attaatcact gatattcata
      601 cagctgctaa agtttatggt aataccgaag atacagctgt ataccaagat gcaacattct
      661 ttgtaaaagg tgataagaat cttgatagtg taataaaaga tcttgggaaa ttagatataa
      721 attggagaga atataatttg attaaaagtt catcaaatta ccctgcatta caacaatcta
      781 tatcaggtat ttactcaatt tcaaataaat tattttttgg ctcattaata tttgcaggag
      841 ttgtagtttc attattatta ttcttatgga tgaatgctag aaagaaagaa atagcagtat
      901 tactatcatt aggtatatca aaattagaaa tttttggtca attcctaatt gagatggtat
      961 ttatatcaat ccccgcattc gttggttcct atttcttagc tcaatataca gctgacaagc
     1021 ttgggaataa tatattgaat aaggttactg gtgatatagc taaacaaata gctaaacaat
     1081 ctgcatctag ccaattaggt ggtggggcag aagctgaagg atttaacaag acattatcaa
     1141 gtttagatat caatgtatta cctaaattca tgatttatgt agttatattt atgagtttag
     1201 tacttcttgt atcattgatt atttcttcat tcaatatatt aagaaggaat ccaaaagaat
     1261 tattaattga caataattaa aattttcggt gctttttagc accaaaaatg agagtgtaat
     1321 ataaaccgtg taagtaacca tcaatcctag gcgggtggtt gcttcacggt ttttattttt
     1381 ctaaaataag atcaagaggt gattacacga tggctagaaa gaaaagaaat cctgatgctg
     1441 agaagttagc tgaatccatt ttgaatactt atcaacccga atctgtagag gatatgcaag
     1501 atgttttaaa agatgtgttt ggtcccttat ttgaaaaaat gcttcaagga gaattgaata
     1561 accatttagg gtatgacgcc cattctaaag agccaaagaa acatgacaat cgacgaaacg
     1621 gttatggcaa taagacgctt aaaacgagct ttggtaaagt aaacattgat gttcccagag
     1681 accgggaggc ttcttttgaa cctgagttga ttcctaatag aaaacgagat gtctccgaca
     1741 tcgcagactg tagacaaaag ctgttccaaa ctcgtaactc gagcttggaa cagcttttta
     1801 tctacttcgt ttcgaatttt tgaacaaaaa aataaaggct ccttgccttt ccatagccag
     1861 gtggctaatt tctttaaatt cattgcagca aacactagca tcgtatgcgt cgcgactttt
     1921 tccaagcctc gatacttggt ccaacgcatc ccatgctttt cttttgcatc cgcaaatatt
     1981 cgttcaatcg tttgtttccg ttttttgtaa attgatttat tcagatctgt taggcgaaga
     2041 tgttccactt catccatcag atctccccaa acatgtctcg tgatg
//
LOCUS       DAJSKS010000230         2074 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJSKS010000230 DAJSKS010000000
VERSION     DAJSKS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.239"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(605..895)
                     /locus_tag="ON396_002776"
     CDS             complement(605..895)
                     /locus_tag="ON396_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114812.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1103..1794)
                     /locus_tag="ON396_002777"
                     /pseudo
     CDS             complement(1103..1794)
                     /locus_tag="ON396_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          588 a          388 c          307 g          791 t
ORIGIN      
        1 tgatgcaaaa gaaatctacg atagtcaaca aaaaaatttt cattatatcc agaaaaattt
       61 ttcttagtta aggacctatg gattgcagta atgcagaatt cgtcgaataa actgccaaag
      121 acatacaatc atattgcttt taaaattgac gaacaagata tagattcgtt tgtctcaaaa
      181 atccaaatgt taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg cgaggcttct
      241 tcaatttact tttacgacta tgataatcac ctcttcgagt tacataccgg tactctccag
      301 gaacgtttaa aatcctacaa ttccactacc tgagactagt aatgactagt ctctttttat
      361 ggcctctgat ccccccattg agcgaatact aatagggctt cgaattgatc tgttcaatct
      421 ttaagcaata tccctcaaat gagtttggaa agtttagtga ttcatcacaa atacttaaat
      481 acatgtagtt atatatagat tgaggttctt gtgagcgtat gctcgtgtgg gttcaaatcc
      541 catcgtctgc atccatcttt ccaaaacaag cggctaggac atttttgtct cagccgcttg
      601 ttttctagtt tattctttca tcttgtcgga ccttttttct tgggtataga cttttctcat
      661 attctaccac aattttgggt ttccctaaca catgatagga tctcacaaca aattgctttc
      721 tattattata ataacctgta acgactaact tcatcccttc gtctacttca gctaggaagt
      781 tcaaactatg ggaagcaatc aaacaattga ccccatttaa actgaaacgg atcaacggtc
      841 tggcactaac ttgcaaagta cggatcttat ctactgttcc atattttttc tccataacct
      901 tcacctcaat accattatac gaacatacat tccctttgta aagaagaaac tctgttttat
      961 ctttttgact ttttgtctat tacctttctt aaaatcatat tggcacttcc tgcgaagatg
     1021 aaggacatgt actaaaaaaa agaggcagca gtatagatta ctgttgcttc tttactaatt
     1081 taggtttact tgttaagaaa gattatttac gcaaatgttc ttcaacaatt tgctgagatt
     1141 tatcagctgt atcttccact tcatcttttg ctttttctaa cttttgaata tattgtttca
     1201 agcttgagtt ttcagcagtt gcttcgtcta atttcttttg tgtatctgtt aactgatttc
     1261 tacatttgtt ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt
     1321 ttgtaattct gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag
     1381 atgattgatt tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg
     1441 tttgctttgt agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc
     1501 tgccgattga tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc
     1561 tgcctttgaa tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat
     1621 cgcttgctct ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc
     1681 aatatctgtc tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc
     1741 aattagacat agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat
     1801 agctccttaa tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag
     1861 cgtttttaaa ttacatatgt aattttaata taaatactat caaatactca tttaatcctc
     1921 cttttttatc tatttaaaaa atctttttta tagagaaaaa tgcaggttaa tactttctta
     1981 aataaatatt tatggtgaac aaaaacaaaa aagcaagttt tattaaacgt aacccttttc
     2041 atttcataga ttactgtatt tattacaaaa aacg
//
LOCUS       DAJSKS010000231         2013 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJSKS010000231 DAJSKS010000000
VERSION     DAJSKS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.240"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..655
                     /locus_tag="ON396_002778"
     CDS             <1..655
                     /locus_tag="ON396_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR3114813.1"
                     /translation="LKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFDDKQNKMQD
                     LKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIAVHPSAIVA
                     KEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPGVCLAGGVH
                     VGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKKEGINFEF"
     gene            645..1787
                     /locus_tag="ON396_002779"
     CDS             645..1787
                     /locus_tag="ON396_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR3114814.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            1800..>2013
                     /locus_tag="ON396_002780"
     CDS             1800..>2013
                     /locus_tag="ON396_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3114815.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSP"
BASE COUNT          717 a          265 c          417 g          614 t
ORIGIN      
        1 attaaaatat actagtgatc catacattat tattggggct ggcggacacg caaaagtcat
       61 tattgaaacc ttaagattag aaaataagtt aatatatgga ttttttgatg ataagcaaaa
      121 taaaatgcaa gatctgaaat atcttgggaa aataaacgaa ttgaatagct gggcggatag
      181 caaaaaaaaa atctataaat ttattgtagc tattggtgac tgtgtagtaa gaaaaaagat
      241 tataaaaaaa ttgaatatgc caagtgagca atatggaata gctgttcatc caagtgctat
      301 agtggcaaaa gaaatttcaa ttggttatgg aactgttatt tttgcgaatg ctgtaattaa
      361 cccagatgct gtaattggag agcatgctat aataaacaca ggtagcattg ttgagcatga
      421 ttgtagaatt aataactatg ttcatttatc tccaggtgta tgcttagcgg gtggagtaca
      481 tgtaggtgta ggaactcaaa taggaattgg ttctcaatgt atccagatga agaaaattgg
      541 atcttggtgt atgataggcg cagggagcac tattgttaag gatattccct cacattcctt
      601 agcttatggc aatccagcta aaattaagaa agaaggaatt aattttgaat tctagaattt
      661 tattatcaag tcctcatatg ggaggaacag aagaaaaata tgttgctaag gcttttaaag
      721 ataattggat agcgccatta ggtgaaaatg tggataaatt tgaagaaagt attacaaaat
      781 atactggttc taagggcgga gcttctgcgt taagttcagg tacagctgcc atacatcttg
      841 ccttaagact gcttaatgtt ggaaaaaacg atattgtctt tgctccttca tttactttta
      901 tagctacagt aaatcctatt ttatatcaag gggctgtacc agtgttcatt gactcagaac
      961 ctaatacatg gaatatgagc cctgttgcac tagagagcgc attagaagaa gctgaaagaa
     1021 acaaccaatt acctaaggca atcatcgtag tgaatttata tggtcaaatg gctgattatg
     1081 aacgaatttt agctattgca aaccaattta atgttccagt gatagaagat gcagcggaat
     1141 cgttaggttc tacttataag ggaatttcgt caggcttatt tgggacaatt gggatctatt
     1201 catttaacgg taataagatc atcacaacct caggtggagg agctctagtg tctagtgata
     1261 aaaaatatgt tgagcattca agatttttag caacacaagc aagagatcaa gcaccgtatt
     1321 atcaacatag cgaaataggt tataattacc gtctaagcaa tatttctgcc ggtatcggac
     1381 gtggacaaat ggaagtattg aatgatagag tagctaaacg aagagaaatt ttcaatcgat
     1441 ataaggaagc tttttctgga attgatagaa ttgaaatgat gccagaagtg gatggcatgg
     1501 tgccaaatcg ttggttgtct actttaacga tcaatgatta taattctatt gatcaagtag
     1561 ttaacgtaat aaataaacta aatgaaaaaa acattgaaac aagagctttg tggaaaccac
     1621 tacatttgca accagtattt caaggggtaa aaatgtatta ccatgataat aatcttagtg
     1681 tgtcagaaaa gttatttaat agcggcttat gcttaccttc tggaagtaat atgacagata
     1741 gtgaacaaga atttgttatt gatgagttta aaaaaatatt caagtaggtg tgatatttta
     1801 tgaacatagg aattattggt ttaggttcca tggggaaaag aagacttaga ctcattcaag
     1861 aacactttaa tgatatttta ctatgtggtg ttgatactaa tgaggcgaga cgaaaggaag
     1921 ttgaaaagga atttggaata ccaacttata atagtctaac agaggcaaaa aatttgtttg
     1981 aaataaattc agtttttgtt tgtacatccc cta
//
LOCUS       DAJSKS010000232         2003 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJSKS010000232 DAJSKS010000000
VERSION     DAJSKS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.241"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..378
                     /locus_tag="ON396_002781"
     CDS             <1..378
                     /locus_tag="ON396_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR3114816.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="ON396_002782"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="ON396_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR3114817.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="ON396_002783"
     CDS             1859..>2003
                     /locus_tag="ON396_002783"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114818.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAJSKS010000233         1993 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJSKS010000233 DAJSKS010000000
VERSION     DAJSKS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.242"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..>1993
                     /gene="acm"
                     /locus_tag="ON396_002784"
     CDS             <1..>1993
                     /gene="acm"
                     /locus_tag="ON396_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HCR3114819.1"
                     /translation="VHVYADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEKKQNI
                     QPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKNLDSV
                     EGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTGDMLP
                     EDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYRGEEG
                     IQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFENHSEA
                     WFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTFELRR
                     ADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELKFSIN
                     ENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDAETKR
                     LDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNENKEV
                     ISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPKYRDG
                     QEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTEREKIR
                     NLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSI"
BASE COUNT          785 a          330 c          396 g          482 t
ORIGIN      
        1 gttcatgtct atgcggatgc aggcagagat atcagcagta atgtcacttc gttgaccgtg
       61 gacccaacga atattacaga tggaggaaac ataaaagtaa aattctcgtt tgacgaaaaa
      121 aaacaaaata tccaaccagg tgattatctt tggatcaatt ggccaagtga aggaaatatt
      181 cgcggtgaag gattccaaaa agaaatccca ttaatgattg aaaataaaaa tgttggaact
      241 ttaacggtca gaaaagattc tgcgcaggtt gtgtttaatg aaaatattaa aaacctagac
      301 tcagtagaag gttgggggca atttgagata caggccagaa acgtaaccga taccggtgaa
      361 gaaaatacgg gaccattcac cgtgacgagc ggtgataaaa cagctactgt aaatgtgaca
      421 aagccagctt ctggttcttc ttctagcgtt ttttattata aaacaggaga catgctacct
      481 gaagatacta agcacattcg atggtttttg aatattaaca ataacggcac ttatgttgaa
      541 caaccagtta aaatcagtga tgaaatccaa agtggacaaa gattagatcc aagtacgttt
      601 gaaattaatc aaatacactt aggtgaacag aaggtttata gaggagaaga gggaatccaa
      661 caatttttac aagatttccc cagcgctaca tttaattttt cagtaactga taattacatt
      721 gaaataacta taccaaaaaa ctttgtcaat cttaggaaaa taatggtgag ttataagacg
      781 atcatagaaa acccagaaca aataaatttt gagaatcatt cggaagcttg gtttaaagaa
      841 tttaataaac ctgctgttga tggagaaagt ttcaatcata cagttaaaaa tatttcagct
      901 tctggtggag tcaacggtac agttagaggc gaattgaaga ttttcaaata tatcaatgac
      961 acagagattg gtatcccaaa tgtgactttc gaattaagac gagcagatga acagcctatt
     1021 caaggacagt ctagtatcct gttgacttcg aatgaacagg gagaagctag tatcaaaggc
     1081 ctgcaagtag gagactatgt tgtaaaagaa aaagaagcac ccaattggat tgattttgat
     1141 ccactaagtt caaatgaact caaattctcc ataaacgaaa atgatacaga gggagtcagt
     1201 ttacctattt ataacaagaa aaaggttacg aacatcacag caactaaaat ttggaacggt
     1261 ggaaccactc caagaccgag tatttatttt aaattattca gagcaagtac aaacaattgg
     1321 gaaccagttc ctgatgcaga aacaaaacga ttagacaatg gaataacttc agtgacttgg
     1381 gaagatatcc aacagtatga tgatagtgga aacgaatata cgtttaaagt gcaagaagta
     1441 gatcaaaacg gaaatgatta tgtcccttca ggttatcaaa aaattgaaaa cgggctagtc
     1501 gttacaaatg agaataaaga agtcatttca gtctcaggac aaaaaacatg ggaagacaat
     1561 gagaaccaag acggcaaacg accgacgacg atcacagtaa atctattagc cgacggtcaa
     1621 ccgatccaac acaaagaagt aagtgaaaaa gatgattggc gctatcgttt cacaaatctg
     1681 ccaaaataca gagacgggca agaaatcatc tatactgtaa cggaagacaa cgtgcccgaa
     1741 tacagtacga cgattgacgg gtataacatc aaaaacagtt atacaccgga aaaaacaaat
     1801 atatctatac caggtagcca tataacaccg tggaaaaatt ttccagagaa gacagagaga
     1861 gagaaaatta gaaatctatt ctctcaaaaa ttgcaaatat ttgctcatac aggagaatcc
     1921 agcgaacaaa gaagcaatta tattagtaag agagctgtac ctaaacagat aacaaataaa
     1981 tcaaaaagca ttc
//
LOCUS       DAJSKS010000234         1979 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJSKS010000234 DAJSKS010000000
VERSION     DAJSKS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.243"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(325..528)
                     /locus_tag="ON396_002785"
     CDS             complement(325..528)
                     /locus_tag="ON396_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114820.1"
                     /translation="MSQLGKRYSLSKSNRKVYTLFISDSVKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(728..1744)
                     /locus_tag="ON396_002786"
     CDS             complement(728..1744)
                     /locus_tag="ON396_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCR3114821.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAJSKS010000235         1978 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJSKS010000235 DAJSKS010000000
VERSION     DAJSKS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.244"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            51..260
                     /locus_tag="ON396_002787"
     CDS             51..260
                     /locus_tag="ON396_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114822.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="ON396_002788"
                     /pseudo
     CDS             467..667
                     /locus_tag="ON396_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..>1978)
                     /locus_tag="ON396_002789"
     CDS             complement(1379..>1978)
                     /locus_tag="ON396_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3114823.1"
                     /translation="KIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRPEF
                     QKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGNEL
                     LDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQKR
                     LVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
BASE COUNT          640 a          338 c          355 g          645 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttt
//
LOCUS       DAJSKS010000236         1947 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJSKS010000236 DAJSKS010000000
VERSION     DAJSKS010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.245"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            324..941
                     /locus_tag="ON396_002790"
     CDS             324..941
                     /locus_tag="ON396_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3114824.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            982..1281
                     /locus_tag="ON396_002791"
     CDS             982..1281
                     /locus_tag="ON396_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HCR3114825.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1315..1482
                     /locus_tag="ON396_002792"
     CDS             1315..1482
                     /locus_tag="ON396_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR3114826.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1527..>1947)
                     /locus_tag="ON396_002793"
     CDS             complement(1527..>1947)
                     /locus_tag="ON396_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3114827.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          654 a          309 c          381 g          603 t
ORIGIN      
        1 agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa ataaaatata
       61 ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag accgttaagg
      121 cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg taaacttact
      181 gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta tggcataata
      241 accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa acaattttag
      301 tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc agtagttatg
      361 atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct gaagaatata
      421 tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa ttaatgaaac
      481 ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta ggcagaaata
      541 aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt gatatttctg
      601 tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg ttagagacgc
      661 ttatctctga cttaattctg caattactca gctatatggc cgaagacgag cggaaacgaa
      721 ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta aaattcggca
      781 gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg aaagcaggaa
      841 agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc ttttatcgac
      901 gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa cagctattag
      961 acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt tactggaacg
     1021 ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa cggccattgt
     1081 caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc taagtcgttg
     1141 tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat tgcgttaggt
     1201 catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca acagttgaaa
     1261 gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat ttttatgaag
     1321 cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa tattgctttt
     1381 cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa ttacttttac
     1441 tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa tagtgcggta
     1501 gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat tttataaata
     1561 gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc ttgctttaac
     1621 atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt aatagaatac
     1681 tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc ttgccgctga
     1741 cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga taaaaacata
     1801 tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa ctgtgcatct
     1861 aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt tatatcttca
     1921 taatctcttc ctaaacgatc tagtgat
//
LOCUS       DAJSKS010000237         1944 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJSKS010000237 DAJSKS010000000
VERSION     DAJSKS010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.246"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            557..889
                     /locus_tag="ON396_002794"
     CDS             557..889
                     /locus_tag="ON396_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCR3114828.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1508..1849
                     /locus_tag="ON396_002795"
     CDS             1508..1849
                     /locus_tag="ON396_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HCR3114829.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          673 a          262 c          414 g          595 t
ORIGIN      
        1 ttatttaaac aagctaaaaa tcttgatatt acttcttttc aggcaatacg aatattattc
       61 gcttacattt tttgtataaa tttaattgtc tgctatgctt gtgatgaatt gattttcgtt
      121 ttgcactttt ggatgataaa tttggacaat tgaaaataca aaaaaaagaa aggcggtatg
      181 aactcggaag gctggtaacc ttcgtcagcg ttcatacacg gcattaagat ttggccctta
      241 atgcgttaac ctttcagtgt tacggtatta cccgcaatgg atttagtata acagatattc
      301 aaaagtgata ccactttttt tggctgtgtt aaggttaaaa atacgtgata ttacgatgaa
      361 tctatgccta agcataatgt tataaaaatg tccgtgtggc tctttgccgt atgacgaacg
      421 ctgaaattag agggaacgca tgtttggatg cgttccctct tttttgtttg tcatgaacga
      481 aaatcaattc tctatttttg aaaaataaca agaaaaactt gttaaaaata tttaaagaaa
      541 ttgggtggtt tattttatgt catatacagg aattttgagt ctcaaagata tttgtcacta
      601 cggtaaacgg tgtaccgcta cagagaaaat cacgaagaaa ctatcaactg gccaaagtaa
      661 aaccgttgtg cagtgtaaga aatatataat ccagaaagac aaagtctcag aggaaatgat
      721 ttattacatt gggaaacaaa agcaaataat tttaaaagat cctatcccct taaaagagct
      781 atatccaaca atcaaacatg tttatgacca aaacggtgta ctgattggtc gaagaaaaaa
      841 tggagtcttg cgttgtaccg caaaagggat ggggcgtttg atcggctgaa ttgatcattc
      901 tcataacttg tattttatcg tttttcgctt gcttgaaaat attgtcgaga cttctaaagg
      961 aacttagagt cactagaaaa ataaggagta gacttgatct atcaacaatt cccttcgggg
     1021 agtgacgggg taggttttaa cgattggtaa cgtaaaaaat tggtcaatcg agtaaatgcg
     1081 cttgtttgga tagtggtgaa atgaatttgt gtaaataata agcgcagagt aaacaagagg
     1141 gaatttagaa gttagacaag ttagaatagc gtctagatta atcatgagca gctactaagg
     1201 gatctagact ttgacgcaaa ctcatggaat aaggagtagg tcttctcgta atggggagga
     1261 cgcacaagaa attgtgtcgt gtgctgcagt acatgtagat acttgcgagg acgttgatta
     1321 ttggatcaat cgagcattta ccgctgtcta ctagggtttc tcgtttcgga cgccataaac
     1381 gagtcgaaat tttttatgta tttttaagta aacgaaaaac gataaaatat gaacaagtcc
     1441 tgaataaagt gaaaaaatta atataaagca tagagaaaat gaaatttacg taatggagga
     1501 atgacaaatg aaaaattatg atccgaatat acgattgggc acacatacta ttaaggtttc
     1561 tttccaacgt tgggattaca aaggatttgt gacgtttaga aggggcggaa attgtaaagg
     1621 attagatgtt ttagcattag acgaggatga tttatacgat caaacattga ctgataatcc
     1681 aattggattc ggcttgctac ctgaagatga tgaaggtaat gaatggttta aaatgacgct
     1741 tatgaatgat aatggagacg aattatctgt tgaagatact tggtcatatt tgagtgatta
     1801 tattgtcagc tttgaaatca ttgaatttgt agcggataaa gaagaataaa aactggagga
     1861 ataaatttta cagataaagg cttagaagga gtcacgacga ttaataaaaa ggtagtatta
     1921 ccttaggcat tgagtaagga attt
//
LOCUS       DAJSKS010000238         1939 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJSKS010000238 DAJSKS010000000
VERSION     DAJSKS010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.247"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            268..834
                     /gene="amaP"
                     /locus_tag="ON396_002796"
     CDS             268..834
                     /gene="amaP"
                     /locus_tag="ON396_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCR3114830.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            847..1035
                     /locus_tag="ON396_002797"
     CDS             847..1035
                     /locus_tag="ON396_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCR3114831.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1048..1605
                     /locus_tag="ON396_002798"
     CDS             1048..1605
                     /locus_tag="ON396_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR3114832.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1637..1879
                     /locus_tag="ON396_002799"
     CDS             1637..1879
                     /locus_tag="ON396_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR3114833.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          711 a          301 c          400 g          527 t
ORIGIN      
        1 atagaatctt gtaataataa ctgagcataa tctttgcaat ttttaatctt gaataatatc
       61 ctttaagtat agccagcaat caaaagtaaa tagagaataa gtaagcaatt gttatttcag
      121 tatcagactg gcaataaaac gccgttatga tccagcaatg tttggtagcg ctttatttac
      181 tgatgctatg ctaactgttc gctatctata ctgtgattgt cggtagagtt atttacaggg
      241 taaaaataag tttataggag gtggaaaatg aataaggggt taaaaataat cggtgttatc
      301 atgagtttac tgctactttc agtatttcta gcgattgcgt tgatcaatgc gccgtatatt
      361 atgcctgaac aattggaacg atttcgcttc tttactttaa cgaattacta catgcaacaa
      421 tatattttct gggcagctgt agtatttgcc gttcttgcta ttatcatctt gctagtcgtt
      481 cttttctatc caaagtcaag aggaactttc gttatgaaac gggaagatgg caaactaaca
      541 atcgacaaaa aagcgatcga agggttggta cgttcccatt tacacgaaga agagttcatt
      601 cattcaccta aagtacggat acgttcaacg aaaaatcgaa tccatatcca tgtcaacgga
      661 gacttgaaac gaacttcttc attagtaggg aaaacaggtg tattgatgca agatatcgaa
      721 gaagaagtaa ctaaagtact cggaacaaaa gaaacggtca aagtggcagt cacatacagc
      781 ggttatgaag aacaagaaga tacaagagac tacaagcact ctcgagtgga ataggagggc
      841 caaataatga aagaactatt ttcagcatac aagctcccta ttatctttgg gggtcttgga
      901 ctactgctag ctgttttatg gttaacgatt ggatttttca agacattgtt gatcataatc
      961 atgacagcaa ttggcgcagg aatcggtttt tatttgaaac agtcaggaat agttgatcaa
     1021 ttttttaaat cataggagga aacaaagatg gaaaacaaaa acatgaatac aacaaatgga
     1081 tcagttttgg caaatgaaaa aggagaaaac cgcatgatga atggtgaatt gacttatgaa
     1141 gacaaagtca tccaaaaaat catcggttat gcattgaaag atgtagatgg tctattgaca
     1201 ttagatggcg gatttttctc aaatatggct gaaaaattag tcaacacaga taacgtaaca
     1261 gcaggtatca atacagaagt aggtaagaaa caagtagctg tggatctaga tgtgatcgta
     1321 gaatacggta aagacatcga agatatttac aaccaaatca aagaattgat cagtactgaa
     1381 gtaaacaata tgacacattt agatgttatc gaagtgaatg tgaatgttgc agacatcaaa
     1441 acacaagaag aataccaaaa agatcaagaa acagtacaag acaaagtaac agaagcagca
     1501 aaatccacag gacaatttgc ttcacgccaa acagataaag caaaatctgc tgtaggcaaa
     1561 ggtgcacaaa aagtcaaaga aaataacgaa ccacgtgtac aataagaagt aagaagtcag
     1621 ttaggaggaa tttaaaatgg gatttatttg ggcattaatc gtcggcggta tcattggagc
     1681 aattgcagga gcaatcacga ataaaggatc atcaatgggg atcatcgcaa acatcattgc
     1741 tggtttagta ggttcagcaa ttggacaagc tttactagga acttggggac caacaattgc
     1801 aggaatggct atcattccat cattgatcgg tgcaatcatt ttagtattag tcgtttcatt
     1861 ctttgcacgt aaattataag caaattgaag aaaaacttat gttgaaatga agaacaaatt
     1921 tgagaaccgt agcgacact
//
LOCUS       DAJSKS010000239         1887 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJSKS010000239 DAJSKS010000000
VERSION     DAJSKS010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.248"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(34..735)
                     /locus_tag="ON396_002800"
     CDS             complement(34..735)
                     /locus_tag="ON396_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR3114834.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(751..1191)
                     /locus_tag="ON396_002801"
     CDS             complement(751..1191)
                     /locus_tag="ON396_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCR3114835.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1191..1766)
                     /locus_tag="ON396_002802"
     CDS             complement(1191..1766)
                     /locus_tag="ON396_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCR3114836.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          531 a          404 c          293 g          659 t
ORIGIN      
        1 aatttttctt tcccgtcgtt tggtttttta tttttagcct aattcttgac cttttgcatg
       61 ctggttatcc gtaaaatatt ttccagcagg ctttccttcc aattcagcta atttttcttg
      121 gatctgacgg atcacctttt ttgcatcttc tggattttct acaaaatcga atttatcccc
      181 atccacttgt attttaggac atacatcaaa atcttcatac cattgattat agcgggtgtt
      241 caacgtttca taataggtat ataacgtctc atcgctttct agctgttcat agtcacgccc
      301 acgttttttg atacgttcta gcatcgtgtc caaagaaact ttgatatgga ccatcaaatc
      361 aggccgtttt tttggagcaa catcatctaa ctcgtttagc atcgtatcta ataagctatc
      421 gtactgctgt acttcgatat ccgttacccg acctaaatca gcgtttagat ggaacaacag
      481 gctatcttca tagatggatc ggtctagcac attgtcatcg ttcactaaag catttttcat
      541 tgctaaaaat cgcttgttca aaaagaagat ttgcaataaa aaagcgtact gctcaggatt
      601 tgaataaaag agcggcaata cttcgttatc ttcgacattt tcataaaatg gtttagaatc
      661 aatctctttt gcaagcatat ccgtcaaact gctcttacct gcaccaatcg ttcctgaaat
      721 caaaatcact gacatacaac ccttcctctt ctataagtac tcgcctacgt aagtttcaac
      781 aggcaatgtt tcaaaatcat attcacgtac agcttggtct gattttaaat acgtatggag
      841 actttgtgta atcatcaagt tcgtaggtac cgtctcttct tgtacaatga tcatcggttt
      901 tttcaacata gttgccacac ctaattcgta agccgttcct ggatcgattg tttgatgctc
      961 aaagtcaagg atagcaacaa caaattctgc atttgtcagt tcttccatat ctttttcgta
     1021 tacttcctgc gcccatccag cgctgaatag ttcataattg ctttcttggt gatgacgtgg
     1081 agagtagaaa cttgtgactg ttttgttttc ctccaacgct ttttcgatac gacttactcg
     1141 atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat atttgactca ttattttgcc
     1201 tccttcaata ataactgatt tttaatagat actgctcgta attcattgac cagttccaat
     1261 ggattgttgg aaaagattac ttgatccaac cattttttat ccataaaatc ctcttttgca
     1321 aaagtttcta ataattgttg caagccgctg taataaccgc caatattcaa tattccgatt
     1381 ggcttgtcgg gcgtcagacc gattgctatc caagaaagaa gttgtgagaa ttcctccaat
     1441 gtacctagac ctccagggaa aacgaggaca gcatctccct tttcaaataa taataccttt
     1501 ctttcatcga tcgtttctgt aggaataaat gtagtaacat cttttccagg caattcagtt
     1561 tcaaaaagcc cattcggata gatacctgtg acttttccgt tattttccag tactgtttta
     1621 ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat ttcttcttgt
     1681 gccatgtatt ttcctaacgt ttgcgcaata aatttgtaac tttctttatt accaaaacgg
     1741 gaaccgcaaa aaacagtaag tttcataaaa aaacactccc taactcaaaa gtctatcttc
     1801 attctacacg tgttttttta aaataaaaga ctagattttg tagtttttta gtggtatata
     1861 ggatagacgc cactatatat agtattt
//
LOCUS       DAJSKS010000240         1857 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJSKS010000240 DAJSKS010000000
VERSION     DAJSKS010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.249"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            59..868
                     /locus_tag="ON396_002803"
     CDS             59..868
                     /locus_tag="ON396_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR3114837.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            908..1405
                     /locus_tag="ON396_002804"
     CDS             908..1405
                     /locus_tag="ON396_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR3114838.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT          646 a          334 c          353 g          524 t
ORIGIN      
        1 tcatttaatc ttaaggtaac aggaaaatag atatgtaaaa agaaagtagg aagataaaat
       61 gaaactagca gcgattgatt tagacggtac cttgcttgat cgaaatggaa agataccaga
      121 agaaaatatc caagcacttc ggaaatttga tgaaaatggc ggaatcgtaa ccgtcgctac
      181 cggaaggaac agcatttctg ctaaagatat tttttccgaa ttaggaatcg gtggctattt
      241 gatttcttcc aatggttcac tgattgctga aatgaaggat ggaaaaatgg cgaaagttct
      301 cagacgttca aaaatagata gcgatacact gagaaaagcc ttcttctatg ctcaagaagc
      361 gaaaatatcc atcattgcta gccgagaaac ccaagatgat cagctgacat tcgatgaatc
      421 aaatctagtc aaagatgacc cttattatga acatttcaat ttacagaacc attcttttga
      481 tcaaatttta aatatgttgg aagatccttc tcttagctat cttaaattag cattaacaga
      541 taagagtgaa gaaaaactac agaagctaca aagagatctg caagcagatg gtatccaatc
      601 agtattctct gatccgcatt tcttagaaat cacgcctaca ggtatcacta aagctcattc
      661 acttgttttt ctggcggatt accttggaat cgaatcgagt gacatcatgg cttttggcga
      721 tcaagaaaac gacattgcta tgttgaatta cagcgggttg agtattgcga tggggaatgc
      781 acagcgacaa gtaaaagaaa ttgcaaacca agtaacactg acgaatgaag aagctggtgt
      841 tgcttatatt ttaaaaaact tctattaaaa agaataaaat gacgatatga atgtaggagg
      901 aaagaaaatg ttagttatta ttgatatgca aaatcatatt cttgatccaa acagcgaatt
      961 ttatattcca gagtcagatc aattggcaga acgtattgct caaagattag ctaaagcacg
     1021 ccaaaataat gaatatattc tcttcacacg ggatatacca gttgaactga aggacaaaga
     1081 agaagaacga gaagatttgc aattgatccc tcttctttct ccacaagaaa atgaacgagt
     1141 gattaaaaag tattacttta cgattccacc tgaaacttta gctgaaatcc aggaaacttt
     1201 atttgaaagc aaagaagaac aaaaagagat cgaagtagtc gggattgaaa caaacttgtg
     1261 tgtactctct aatctgatag gcctgcagag tgcttttcct gaagcagact tttttgttga
     1321 tccaatgtta gtctccagta gaaaacacgg agaatccgct ttagagctgt tgaaagattt
     1381 caacgtctct atcgtcgaat cataaaaata tctatcttca tttacgatat ttaacacaca
     1441 ataaaaaaag cacgcagaac tgttttagtc ctacgtgctt tttctatcaa agtagattgg
     1501 cttttttatg acacagagtg aaacggcagg ctcacgacct ctttcttaaa tggtgttcaa
     1561 tcagctgacc ctcggtatta gcttaacaaa cggcaaaata tgaggaacta tttttccgtt
     1621 tgttaagcta atactcaggt caaaacgctg atttcacaac ctcttcctta aacggtattc
     1681 aaaagcttgc atctgcagta ataagaacag ccaagtaaaa acataagaag ctgtttttac
     1741 ttggctgttc ttattacttg atgcagggca gctttttcac aacctcttat gtaccttgta
     1801 ggactcgaac ctacgaccgg acggttatga gccgtctgct ctaaccaact gagctaa
//
LOCUS       DAJSKS010000241         1766 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJSKS010000241 DAJSKS010000000
VERSION     DAJSKS010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.250"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(227..1693)
                     /locus_tag="ON396_002805"
     CDS             complement(227..1693)
                     /locus_tag="ON396_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3114839.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT          562 a          359 c          309 g          536 t
ORIGIN      
        1 aaaaacaagg gactgtgaca aaagtcgtgt caaagtccct tgtttcgaat aaatggtgtt
       61 caatcagctg accttcagca ttagctcaaa aaacggcaaa atatgaggag ctatttttgt
      121 ttggctgttc ttattacttg atgcaggaca gctttttcac aacctctgat tcacggtgtt
      181 ccagaagtag cttctgtcac aacctcttgt aacaagtatt tctattttaa ggctgaacca
      241 ctgtttctgg ttcagtaatt tctttgtttt tgtctcgacg agcaattgct ggcaagatca
      301 tacctaataa gatcaagaca attggtgtga tgatattcgt gaataattgg aaccaccatg
      361 ctttaggatc agcagtataa tctacttttg gcaccattcc taggatacat gcaaacgcag
      421 tgaaggcaaa gcaccaagca ccaaagataa aaccgatttt tggattttta acaaatttat
      481 attcagaatt gaatttctta taagctttat tcaacatcat atatgccaag aacacccata
      541 ggtaacgcat tggcatgaca actgagttca agttcgtcaa ccattttact aattcattca
      601 tatctttgat accaaagatc ggcaacaaaa taatgattcc tactagtata cctgtcagaa
      661 gataaccatt caccaatgta cctttacgat tacgtttacg tagccagttt ggtacaaatt
      721 ctggatccgc atctgctaac aagattttta gtggtgcatc aattgaaaag gctaatgcgg
      781 agatttgtcc aatcatgttt gtcaatgcgt aaatgatcat caatgagtta ccgataccat
      841 agtaattacc taaaatttga aaagcactgt atgccccatt tgccatcaag tctttaggga
      901 tattatcgct agcaaacagc atacccattg cgactgagcc taatacagca caaatcccta
      961 ccattgctgc taagaagatc atccctttag gaaattcctt tgcaggattt cttgtagagt
     1021 tgacataagg ggatattttt tccgcccctc caactgcaaa tactagcatc gaaacggttg
     1081 tgaaataact gaaatcaaat gttggaatat atgttttcac ttgattcata tgtggtgtgg
     1141 caaattccat cgttggattg atgaacggtg ccgttacagc taaaagaata aataagatcg
     1201 acatgacgaa catcgctgtt ccggccagtc cgccaatcac tttcaaagta gaaagtcctt
     1261 ttgttgataa atataaaaag gcaagaaata taataagtga aataactgct accatttgta
     1321 ctgacatcgt atttacgata tttccatttc cttggactgc ccaaccacca gcaatcagaa
     1381 ttgcttgagg cttttgtgcc aaataaggaa tatggacgac ccagtaagtc caagcggcat
     1441 agtatgccag tcgtttatta gatgtcgtat tttcgaccca tgaactgacg cctcctttac
     1501 tatccttgaa ggctgatcct aattgtccaa cgatcaatgc gtatggaata aaataaatgg
     1561 ccaaaatcaa tagccaagag gtgacaactg atactccttg ctgagcatag ttattaacta
     1621 cattccccat gccccaaacc atgttaaatg caattaacgc aacagtaaac cagcgtaatt
     1681 gtttgttgtt cattcgttct ctcctttatg ataaaaattt gttatccaga tgtaatcata
     1741 ttcaattttt aacaaactta caagac
//
LOCUS       DAJSKS010000242         1747 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJSKS010000242 DAJSKS010000000
VERSION     DAJSKS010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.251"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            96..389
                     /locus_tag="ON396_002806"
     CDS             96..389
                     /locus_tag="ON396_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114840.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            382..678
                     /locus_tag="ON396_002807"
     CDS             382..678
                     /locus_tag="ON396_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114841.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            753..>1747
                     /locus_tag="ON396_002808"
     CDS             753..>1747
                     /locus_tag="ON396_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HCR3114842.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMI"
BASE COUNT          669 a          239 c          344 g          495 t
ORIGIN      
        1 ttaaaaatac atgtgatgat acatgttata tgaattatca atgtacccag taatatgcta
       61 ttttcattat gtaaaaaata acagggggtg tgtagatgga gattgatgaa acttgtttga
      121 gtaactctta taaaagtatg aatgactttt ttgaggaaat ggaactggat tcagaattgc
      181 tctatcaatt ttatcttgct tttcgagggc aacagatttc ttttccaatg aaaatgtacg
      241 atcgtgagtt agttagaaaa agaatcgaaa acatgataga acaggaaaaa actgtagata
      301 tccgtcaatt gacagaagtt tatggtttct ctgcaagatg gattcaaaaa gtaattaaac
      361 aaaaagaaag gagacgagta catggctaag ctgattgtat taggtggttt aatttacggt
      421 ggatattggt tatatcaata tatgcttgcg catccgaata tagataaagg aaaagtaatt
      481 aagacagttg gggtaatcat agttggactc atagctctaa ctaagaaaaa taatactaat
      541 agcccaaaaa taatgagaac ttatcgctgt agaggatgtg gaagggaaat ttctactagt
      601 tttaatccat ctgagagtcc tcaaggaaga gaccgctgcc atacgggcta tcgacattat
      661 tgggaaagaa tcgattaaaa gattaccatt tagatcaatt aattgtctga tagaataagt
      721 acgtgaggga gaaattgaaa gggggaaata taatgaaaaa atggctgtta ttactaatag
      781 gaatgggaat tttattcact ggttgtacat ctgagaatac gaggactaaa gcgactatag
      841 aaactgatac aacaattgag actaaaaaaa gtaaaacgca atcaactgaa caaactacaa
      901 cagaaatgaa gcaatctgtg gaaacaagtg caagttcaat cactgaatct tcttctgaag
      961 aggttaagaa tactttgtgg gatacgaata aagcttccaa attagaaact tttgtcacac
     1021 aatggggaaa gacattaggt caagaatata aaagctacaa tcttcagaat aatgtaagtc
     1081 tgtatggcac tcctttacca caagccgtga ttaatggcga ttggaaaatg gcaatcaatg
     1141 aagcaccagt taccgtacaa tggtcagaag atggaactgg tcatgcagat ttcaatttag
     1201 tagcagttta ttctgatgta gaaactggag aatatttagg gcagcatgtt tactttttcg
     1261 gttttcaaaa tggacaacct aaagtttatt taacgcaaca aaatcaaggt aatgaaaata
     1321 attatctgta ctttaacgaa actcaaaatc aacagttaaa acaaggtttc agtgatattg
     1381 tgaatggaca aactcctcaa acaccagtag tagaagaatc tactcaacaa gcaacaacta
     1441 caacgacaca atctacaagt agttcaggaa tttcaagttg ggaagctgct aaagaatctg
     1501 tgctaaataa taaagcttta tggttgtatg attctcctga taaaaatgta tcaaatacta
     1561 atttaattag cacaaatgaa tatatgaaag atctaatgca agatgaaaat ggaagctact
     1621 attcaattaa tattgtaacg gatgatgatg gaaacacgac catggctggt ggaaaacaat
     1681 ttcgtgttta tacagatgga agaatttctc aacgtattgg aatgactaac aattttcaaa
     1741 tgattaa
//
LOCUS       DAJSKS010000243         1677 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJSKS010000243 DAJSKS010000000
VERSION     DAJSKS010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.252"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..425)
                     /locus_tag="ON396_002809"
     CDS             complement(<1..425)
                     /locus_tag="ON396_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HCR3114843.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="ON396_002810"
     CDS             complement(534..1565)
                     /locus_tag="ON396_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3114844.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          521 a          291 c          290 g          575 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagcta
//
LOCUS       DAJSKS010000244         1620 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJSKS010000244 DAJSKS010000000
VERSION     DAJSKS010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.253"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            15..497
                     /locus_tag="ON396_002811"
     CDS             15..497
                     /locus_tag="ON396_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HCR3114845.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            534..1544
                     /locus_tag="ON396_002812"
     CDS             534..1544
                     /locus_tag="ON396_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HCR3114846.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT          582 a          254 c          349 g          435 t
ORIGIN      
        1 aaagagacat aaaaatgatt gacttggcaa agaaattctt acacgaaaat atatattttg
       61 agttgtatca tcatcgagcc atttatacaa atgaagatgc tttggtaata aagaaagaac
      121 aaggatttac aggtacagag accaaaagct tattcttaaa agataaaaaa gataataact
      181 acgtatatct gacttttact acgaaacaaa cggatttcaa agcattaggg aagatcgtcg
      241 gtaaaaggct ttctattgtt tcatcagaac gcatggaaga acaaacggga caaaagcagg
      301 gcgctgtttc tccgtttggt tatgaaaaac agattccgat tattattgat gaagagctct
      361 tatcaaatga aaaacttgtt tttgcaccag gtagaccaga tcaaacgatg gtcatccaaa
      421 cggttgactt aaacaaaatc atccagctac ttgagatgcc gatatttttg atgccggcaa
      481 acgaaaaaaa caattaacca agtaaaatag tgcatacttg gaggtgaagc aacatgataa
      541 gttatccatt attagaaaga aatgcaacga ttggggttac tgcaccatcc tcaggcgtat
      601 cagaggaaca ccattcgtta ctcgaatcag ctttaaagag aatggaagaa aaatctttcc
      661 aagtagttac tggaaagacg ctttggacgc aagataagga aaaatcaggt ccagcaagaa
      721 aacgagcaga tgaattcaat caaatgatgc aagatgaaaa tatccaaatg atctttccga
      781 cttggggagg agaattattg attgagattc tcgattttgt cgattttgat gctctacaac
      841 caaaatggat agtaggttat tcagatatta gtacgttgct acttgctgtc acattaaaaa
      901 cagggatagc cactgcacat ggaactaacc ttattgattt aagaggagag tacttggatg
      961 aaacgacagc aatgtgggaa agtgtactga aaacaagaga aggagaatcg attctacagc
     1021 aatcttctga aaagtatcaa aaagtgtggc cccatgagca ttcatcatct tctgttttcc
     1081 atctcactga accaacaaat tggcaatcag tcagcggaga tgcagtaagg atgaagggac
     1141 gattattagg tggttgtatt gatacgatca ggcatttaat cggtactcct tatggagatg
     1201 tccaatcttt taaaagaaag tataatgatg gaaagccgat cttgtggttt ctagaaaact
     1261 gtgagctatc gtcagttgat ttaagaagat cacttgttca gatgaaactg gcaggctggt
     1321 ttgatggcag ctcggggatc atgttcggta gaagtgcggc agaggatttg ttagattata
     1381 aaatgaagga tgtatacgaa gatctgtcaa aggaattaaa actaccgatc ttgtatgata
     1441 ttgattgtgg tcatgtaccg ccacaactga cattgattaa tggtgcctat gcagaagtag
     1501 cggtagaaga tagaaaagcg actgttttgc aaacgtttat ttaaaaaatt ttttcctgcc
     1561 ttggagtcta tcaaagacca ataaatctaa atagaaaaga aagtgaaaga agctgacaaa
//
LOCUS       DAJSKS010000245         1610 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJSKS010000245 DAJSKS010000000
VERSION     DAJSKS010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.254"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..843)
                     /locus_tag="ON396_002813"
     CDS             complement(<1..843)
                     /locus_tag="ON396_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HCR3114847.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDE"
     gene            complement(1002..1268)
                     /locus_tag="ON396_002814"
     CDS             complement(1002..1268)
                     /locus_tag="ON396_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3114848.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1258..1605)
                     /locus_tag="ON396_002815"
     CDS             complement(1258..1605)
                     /locus_tag="ON396_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010490045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3114849.1"
                     /translation="MSFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNGMLV
                     AQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISELKH
                     AKELREEWFTNDD"
BASE COUNT          453 a          314 c          197 g          646 t
ORIGIN      
        1 ctcatcaaaa gaagaatcgt attcttcctt taaaattccc gatttactta atttttctag
       61 tacatcaaat actctatcac tcaagtaact ccttaaattg ttatcacttt cccattgaac
      121 tacttttcta taagttgaat tatcagttga aaagatagaa ttttcatcca taaacgcata
      181 tattagcagc aatctttcat cattgtcaaa ttctattgga tcaaaaatat ctaagttgtc
      241 atcaaaatct ttatcagttc cggtaacttt tttgttaatt actgaaggtg tcttgtaaga
      301 aatagaatct aaatcaaagc gttttgattc taaattattt tgtgctttct caattaaata
      361 attcactttt tttgtgagaa accttctgat attgtctttg ctatatgttc cataaaaacc
      421 cttatcttct aaagagtccc tgtacttctt aacttctata tactcttcag ccaaaacttg
      481 tggaactggt tcatctaaga aaaaaacccc tacctcgcct ttgctatttt tataaaagta
      541 atcaatttct gctatagttc ccgatctata attatcaact ggagaaccca acttatggcc
      601 aaaaatcgct aataatatat cactattttc tacaatttgt ttattaataa cactttgccc
      661 agagctatta tttatataag taggaatcgc attcgtttcc catcgaattg gcatcaaaat
      721 tacttttgta ttatctgtat tttctatatt ccatctatat atggtttctt ctacttcatt
      781 cctctttgct gctatatcac ctggtgatgc tataaatact tttattagat ttatggtttt
      841 catttattgt acctttctct ttatgtctct ttaaagtaat ttttgtataa aatttattga
      901 cttttacact ataggacaac ttttatctat ttagtacaaa aaggcccact aaactaattg
      961 attagttagg agcctattac aggtatattt tcaactcgct tttaagcaaa ttctactacc
     1021 agctttttgc ctaatgccct agcgatatca tcaagtttat cgaaagatac gttatcccct
     1081 ctctcaattc gagcaatagt agattgagtt gtatgtgctt tttgtgcaag ctccgcttga
     1141 gttaggcctg cttcctcccg tgcatgataa agagcaaccg cagtttcgag ttttcttcct
     1201 tcacggtcat atgcttctgc aaaatctttg tctttttgct tttcttcaat caatttttta
     1261 atcatcattt gtgaaccact cctctctaag ttcttttgca tgtttaagtt cacttattgg
     1321 tgttttctcc gtttttttag taaatccatg ggtaatgaca taccttccat tgaccacatg
     1381 gaaatagata gctctttgga tgtttgatcc taccttactt cttaattcgt acaaattagt
     1441 gtctaatttt ttcacccatt tcaaccgttg agctactagc atcccattct tttctgtttc
     1501 ttctattgta cgtaaaagct tcgcactatc tttttttggt aatgtttcca accattctaa
     1561 aaattcatca tgaccattag gtcttttaaa tgtttcaaaa gatatttttt
//
LOCUS       DAJSKS010000246         1532 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJSKS010000246 DAJSKS010000000
VERSION     DAJSKS010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.256"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            22..1488
                     /locus_tag="ON396_002816"
     CDS             22..1488
                     /locus_tag="ON396_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCR3114850.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          422 a          289 c          325 g          496 t
ORIGIN      
        1 taaagaattg gggtatagat tatggaaaaa cagcaagaca agggcttctt cgggcagccg
       61 aagggattgt caactttgtt cttcactgag atgtgggaac gatttagtta ttacggtatg
      121 cgtgcattac ttatctatta tatgtacgat acagttgcca acgggggact tggattacca
      181 cgagcaacag cattagctat catgtcgatc tacggatctt tgatctacat gtctagtatc
      241 gtcggcggat gggtatctga ccgagtgtta ggagcaaaac gcactgtatt cttcggtgga
      301 gttttcatta tgcttggcca catcgtcttg gctttaccat ttggtgtaag tacattgttt
      361 gtttcgatgg cattgattat tatcggaacc gcctttttga aaccaaatgt ttctggcatg
      421 gtcggacatc tttattcaaa gactgacttg cgaagagatg caggattttc gattttttac
      481 atgggtgtca acttaggggc attattagca ccactaatcg ttggtacaat cggtcaagaa
      541 tataactatc atttagggtt ctctttagct gctattggga tgttctttgg attattacaa
      601 tacatcatcc aaggacgcaa gacattagac ggagtgggta caacaccgcc gaatcctttg
      661 acaactgaag aacgtaaagg attcatccgt aacttgatca tcgcacttgt tgttatcgct
      721 gcagtattcg gcggggcaca actaactggt catctgacaa ttgacttttt tgtcaataca
      781 atcagtgtat tggggattat cttaccgttg tattacttta tcaaaatgct gacttcaaaa
      841 gatgtaactg ctgaagaaaa accaagagta tgggcttata tcccattatt ccttgcagca
      901 atcattttct ggtcattaca agaacaaggt tcatctatct tagctttatt tgctagtgaa
      961 cgtacacaat ctacgctatt tggtgtaacg atccaagcaa gctggtatca atcattgaat
     1021 ccaattttca tctttattct gacaccagta ttcgtaacga tgtggacgaa acttggtgaa
     1081 cgtcaaccat ctacagtagt gaaattctct cttggacttt tatttgcagg attatcgtac
     1141 ttgctattga tgttgccggg tatgctttat ggtacaagtg gaaaagtaag tccattgtgg
     1201 ttgttgggaa gtttcttcat cgtggttatt gctgaattgt gtctttcacc agttggtttg
     1261 tctgtgacaa caaaattagc accacgagca tttgaatcac aaacaatcgc tatttggttc
     1321 ttagctgatg cttcttcaca agcaatcaat gcacaaattg cacgcttcta cacaccggga
     1381 acagaatctg cttacttcgg tattatcggt gcagtagcaa tcgtcggtgg tatcttgcta
     1441 tttactgtga aagaaccaat caaaaaacta atgggctcta ttcattaatc aatgaacata
     1501 aaaacaggaa ttgcttattt gcagcagttc ct
//
LOCUS       DAJSKS010000247         1523 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJSKS010000247 DAJSKS010000000
VERSION     DAJSKS010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.257"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            236..1405
                     /locus_tag="ON396_002817"
     CDS             236..1405
                     /locus_tag="ON396_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR3114851.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          562 a          217 c          245 g          499 t
ORIGIN      
        1 tattactatg tttgggtttt atagcaaaga aagtttaata agagtttgga cctagcaaac
       61 aatattaatg aggtcattaa atcttattat ctttagtgat ataacgaaat atctacacag
      121 acaaaatgat agcgtaaaat attttgtgta aatagatatt tagggtagaa aaaaagaaag
      181 tccattctgt aaaattaaag ttaccacaac cgaaatttta taggaggact ttctcatgcc
      241 caattttact acagaaatta tggaaacact aatcaataaa ggtgatttag atgaattatt
      301 tcgatgtcat ttagaattag ctgttaattc attattgcag gctgaattaa cagcttttct
      361 tgattatgaa aaatatgatc gtgctgggtt caattcaggt aattcccgta atgggaatta
      421 ttcacgttca ttcagaacag agtatggaga attaaatttg gtaattccta gagatagaaa
      481 tggtaagttt tctcaacaaa ctttacctgc ttataaaaga accaatgact ctttagaaac
      541 cactattatt cagctatttc aaaaaggaat tactatgtct gaaatctctg aattgattga
      601 aaaaatgtac gggcatcact atacgccaca aaccatttca aatatgacta aaatagtttc
      661 tgaagatatt attgccttta aagaaaggtc cttagaatcc agatactctg tcatttttat
      721 ggatgctact cacattcctt taaaaagaca aactgtttca aaagaagcag tttatattgt
      781 aattggcatt cgattagatg gaaccaaaga ggttcttggg tttagtcttg ctccaactga
      841 atctgcttat gtttggaaag agattcttca agacctaaaa gaccgtggtt taaaagaggt
      901 tttattagtc gtaactgatg gtttaagtgg cattaacgat agtattcata gtgtttatcc
      961 aaatgctcaa tttcaacaat gttgtgtcca tatatctaga aatatcgctc ataaggttcg
     1021 tgttagtgat cgacaagaaa tctgtagcga tttcaaatta gtttatcaag caagttcaaa
     1081 agaagaagca aataatcaaa taaggtttat gatagataaa tggaaaaagc agtatccacg
     1141 agtggttaaa ttgctcatga atcctgctat attaaccttt tataatttcc ctccgtcaat
     1201 cagaagaact atttattcaa ctaatttgat tgaaggtttt aacaaacaat taaagaaata
     1261 taccaaaaga aaagagcaat ttcctaatga agaatctctg gagagatttc tagtatctca
     1321 attcaacaac tataatcaaa agtttctatg ccgagttcat aaaggtttta aagaaataca
     1381 tgatacatta gaatcaatgt tttaacttga tcaacagaat gggcttttcc atttacacaa
     1441 aattattgac gctcccgaca aaattatgat cttgcgtaaa gttataaaat attaataaaa
     1501 atattttatt aatgaaaagc ttg
//
LOCUS       DAJSKS010000248         1515 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJSKS010000248 DAJSKS010000000
VERSION     DAJSKS010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.258"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            133..507
                     /locus_tag="ON396_002818"
     CDS             133..507
                     /locus_tag="ON396_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR3114852.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(621..1250)
                     /locus_tag="ON396_002819"
     CDS             complement(621..1250)
                     /locus_tag="ON396_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR3114853.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1384..1456)
                     /locus_tag="ON396_002820"
     tRNA            complement(1384..1456)
                     /locus_tag="ON396_002820"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Thr,seq:ggt)
BASE COUNT          513 a          228 c          265 g          509 t
ORIGIN      
        1 gtctacactc tgagatgtaa gaaatatcat aagatatttc ttacatcttt ttgttttgag
       61 caggacttta gataatttta ttaatatatg ctagcatgct tttgtaagaa aaagaaagag
      121 ggcgtattag ttatgtatct taaaattacg gaacaagcac aagaaagatt acaaaaatat
      181 attgatgaag gcgcaacagt tatattggat ttagatgatg gcgttgggga atattctaaa
      241 atgggtgttt gctctttaga tactagtttt cgtttacttc ttcttgataa agaacagaga
      301 aaaaatgatt ataaactcca attagatagt gatattgggg atgtttatat aaaagattat
      361 tcaaaaatgt acatggatga acatatgacc ctttctttag atccacgtct aggtgtattt
      421 actcttgaaa gcccaagtgg ttctttagac agtcatgttc aattagtaga tctacgacca
      481 gaaaaattct ctggaataaa acaataactt tctctctatt gtccataaaa ctagtctcaa
      541 tcaagctgta ttgaaaaaac aagacaacat tcttaccatg gtaaataaaa agacgacaca
      601 ttttgtcgtc tttttttaaa ttaactattt tcgataaaag atcgcatagc ttgtgccatt
      661 atttctggct tttctctatt caattcatgt tttccgtttt taataaagag tagccggcca
      721 ttcgttgaaa gtttatataa acgttttgct gctggtttat tcgcatgatc tttggtaccg
      781 cataaaatca atttaggaag tcgtgatgta cgaagctgtt ttttgcagtc taatgtttca
      841 agtgaacgaa gtagggtaag ggtttgtttt ttagatagac ctaatttttc aaatatctta
      901 gaaggtacgc agtaaaaata aatattctgg aaagtaagca ataagcgatt aggagactca
      961 aattgtggag cgactaaaac aacctttttt aaatatggag aaggagttgc aagatagttt
     1021 aaagcaataa cagcgcctaa agataatcca cataaaataa aaggttcctt tatctgattc
     1081 aattgtactt cgatgtattt ggttaagtcg tctaaagtag cagtgtctgt gctgtttaaa
     1141 tgattgaaca atgaaagact tttgcaagaa taactattgg gtagataaga gattacatct
     1201 ttccatgatt gttcgtcttg gcctagacca tgcaaaaaaa caattttcat attgctctcc
     1261 tttttatagt tatcatatag actttctttg tactattaaa atataaacat tcatttctta
     1321 cgtcataaaa taaaaaaaca gcgcagacac attgttaaac caacatgttg cactgtttaa
     1381 ggaagccact tgccgggctt gaaccggcga cctcttcctt accatggaag cgctctacca
     1441 gctgagctaa agcggcatta tcattaatac aaataatagg ataacgaata ttaatgtatt
     1501 tgtcaacaga atttt
//
LOCUS       DAJSKS010000249         1514 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJSKS010000249 DAJSKS010000000
VERSION     DAJSKS010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.259"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..267
                     /locus_tag="ON396_002821"
     CDS             <1..267
                     /locus_tag="ON396_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114854.1"
                     /translation="LFNILKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPI
                     QLKDMSYPEEAVREITDNINKLEQTISSIGKKYTKKKMKGGDTI"
     gene            306..1448
                     /locus_tag="ON396_002822"
     CDS             306..1448
                     /locus_tag="ON396_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3114855.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT          603 a          240 c          265 g          406 t
ORIGIN      
        1 cttttcaata tcttaaaaat agaagaatta ttcatcaatg ctattgtaga agaatttgac
       61 aataacattc ttttcctcac tagcagtata gaagaactac tagaaagaat ggtagatgaa
      121 tggctaccta ttcaattgaa agacatgtct tatcccgaag aagctgtgag agaaattact
      181 gataatataa acaagctaga acaaacgatt tcatcaattg ggaaaaaata cactaagaaa
      241 aaaatgaagg gaggtgatac catctagtgg taagtactcc ttgtaaacta agaggagatg
      301 atttcatggc aaaaatatca aagtacaaaa agaaaaacgg tcaagtggcg tggatgtata
      361 aaggacacat tgccaccgat ccacgaactg gtgaaaaaat aaatactacg agaagaggtt
      421 ttagtagcaa acaagaagct caaaaagatt atgacgagta cagacatcaa atattatacg
      481 gtgttaaaaa gaaagcacct gatatgacat ttgaagacct gtataatgaa tggataactc
      541 atcagcgtac cagtgttaaa gcttcaacaa tcgcaatctc agtaagatac gcaaataatc
      601 aaatacttcc agcatttggt aagcttaaac tatcgaatat ctctgtccca tattgtcaaa
      661 aagtggtaga tgaatggcac agtaaatatg aaagttacga ttatatgaga aaacagactg
      721 cacaaatttt acgctacggg gttgcaatgc aatatattga caataatcct atggaaaaaa
      781 cacttcttcc acgaaaaaaa gagtatgaga aaaatcgtaa attttactca aaagaggagc
      841 ttaataattt actggatgct tttaaagatt ttggtaacat gaaacaatac gccttcttca
      901 gattattggc atacacaggt atgagaaaga gtgaagtact cgctttacag tggaaagaca
      961 tagacacgtt taataaagaa ctacatgtca ataaaactct tgctgtagat gaatttggta
     1021 aagttataat tcaatctccg aaaacaagag cttctagacg agttatctct ttagatactg
     1081 agactctttc tatcctcaac aactggaaat tacagcaaaa agaagagtat ttaaaacttg
     1141 gatacaatac tagctctaaa gagcaacatg tttttacaac agttaaaaac actctataca
     1201 ttccaaacac tgttaatgat tggttaagat atattttgaa aaaatataac ttgcctcgta
     1261 ttacacctca tgggtttaga catacacatg ctagtttatt attagaagca ggagaatctg
     1321 tcaaggtagt tcaacaacga ttagggcatg aaaatagtaa agtgacatta gacatctatg
     1381 cacatattac aaataatgca cctaagaaaa caggtcaaga ctttgctgat atgatggctc
     1441 atcagtaaaa gagctggtca aaatctggtc atttctagta tacaggcaaa acaaaaaagc
     1501 tagaaccctt atat
//
LOCUS       DAJSKS010000250         1499 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJSKS010000250 DAJSKS010000000
VERSION     DAJSKS010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.260"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..189
                     /locus_tag="ON396_002823"
     CDS             <1..189
                     /locus_tag="ON396_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3114856.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(275..1369)
                     /locus_tag="ON396_002824"
     CDS             complement(275..1369)
                     /locus_tag="ON396_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR3114857.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT          461 a          305 c          231 g          502 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatggattt tagaaaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgtaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcataac ctataaaaaa acaaaataaa aaagcagaaa taactggcta
      241 tttctagcgg attatttctg caataactaa ttatctattt tttattttca attaatttaa
      301 agcttgacca tggtttcaaa aagtcaagga ggaataattc gtcatcggag attcttccta
      361 caacattgac acgtccgtcg tttttcatat ccttcaaggc gatttgtgtt tctcctttgt
      421 attgaccgta cccttcattg tcaatcagta catccccatg atgcatatct cgcgtattat
      481 gaggcgggaa atctttatct ttgtagtata cacgagtcat cgttgaacga aggatatatt
      541 ctgagcgatc ccctcgataa ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg
      601 taataccttc ttccgtatct actttcaatg ttggataatc cgcgttaaat gcttctgcca
      661 tttctttcaa ttcttcttca cttgcgtacg catttccaat agaaatatca tcaatcaatc
      721 ctgtaaggat taaatgcttc acttgtgtcg caatttcaag atctcggtgg tcttccagcg
      781 tgcacaagcc atcttgcgta ggccaagggc caaactcagc agattgcgaa ttgacgaatg
      841 ccatcgtatt caaattatat tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc
      901 ctaaaccaga gtatcgatga ggataaaaat tatgagaacc taataaatta tccgtgtttg
      961 gcgaataact catgatgttg tcaacgtaat tcgtgcccga actcatattg atctcgattt
     1021 tgataccaaa aggattgcgc gtcatttttg cttcttctgc tcctgtgaat ccgatatcta
     1081 agcggacccc gtaggcaccc atcttatgga aaaaagataa gtcatcgtaa gaaatctcca
     1141 actgttcaaa taacgctgga ttgatatcaa ccattacttc catgcctagg ctattcgcgt
     1201 aatcaactac ttctttaaat tctgaaagaa ctttttcttt atcatcattg atctgcagca
     1261 aactggtaaa aacccgttta tacccatact tatgagctaa atccaagtac gctttgtcct
     1321 tttcaaatgt tgaccgttct ggataaatcg agattcctaa ttttcccata ttaccatttc
     1381 ctcctgcatc ttttcgtttt tgtatccact tacattatag caaacacaaa gtaaatggtc
     1441 tacacctttt ctttttcttt taaaataaat gataaattcc aataatttaa gtaggtatt
//
LOCUS       DAJSKS010000251         1481 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJSKS010000251 DAJSKS010000000
VERSION     DAJSKS010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.261"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            230..844
                     /locus_tag="ON396_002825"
     CDS             230..844
                     /locus_tag="ON396_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3114858.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(868..1218)
                     /locus_tag="ON396_002826"
     CDS             complement(868..1218)
                     /locus_tag="ON396_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR3114859.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1212..1475)
                     /locus_tag="ON396_002827"
     CDS             complement(1212..1475)
                     /locus_tag="ON396_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HCR3114860.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          475 a          269 c          235 g          502 t
ORIGIN      
        1 ataaaatata tttttaatgt cataatcgtt taaattttct tgattgacat ttatttacaa
       61 aaaatattgc catttaaaaa gaaaagtgac acatactatt tcctccatca ttagtttttg
      121 tctatataag gtatacccta actagacacg accgcgacat tgaaaaatct ggttttctcg
      181 tgtatgatta gggtgttttt taaatagttg cagaaagtag gtaaaaattt tgagagttgc
      241 atacatacgt gtctcatcca tcgatcaaaa tgaacaacgg cagattgaag aaatgaaaaa
      301 attcggtgca gagagaattt tcattgagaa acaatctggc gcaacgatta ctcatcgact
      361 tgtttttcaa gaagcactgg atttcgtccg agatcaagat atttttattg tggaagctat
      421 tgatcgactt ggaagaaatt acgacgagat catcgcttca gtcaattact tgaagaagaa
      481 aaatgtgcag ctgatcatca caagtctacc gatcatggcc gaagcaattg gcaatcctct
      541 cctggataaa ttcattaaag atttgatcat tcaaattttg gccatgatcg ctgaacaaga
      601 acgtaccgaa tctaaacgac gtcaggcaca aggcattaag attgcaaaag cgaacggtgt
      661 ttataaagga cgtcctaagc tatacagtgc caatgcaaaa gatccccaac gtcgtcttgt
      721 ttataaaaac atcgttgagg atctaaaaaa aggtgttgcg atcgctaaga ttgcaaaaga
      781 ctacaatgtt acgagacaaa ccgtatatag aataaaaaaa gacagcatgg tcaatcacga
      841 atagttgatc atgctgctgt ttctatttta tttgcttact ttttgaaaaa ctgttctaac
      901 tttcatcaat aactcctcaa ctaaactatc atgcgctttt tccaaaaaaa tacactctct
      961 ggcttcataa tcaattgtcg ttagttgatc taataatact tttcctgttg tcttcatttc
     1021 atattctgag tttatattta catatagcgg atagtttcta gttgtactag aaattggagc
     1081 aactgtaaca acgttactat aatccgcaat aatatcatga cttaaaacaa tataaggtct
     1141 tttacctgtc tgttcgtgtc cagatcgagg tgctgtattc aataatagaa tatcgccttg
     1201 tctaggtttt ctcaccattt ttcattacct actgcttcac ctaaattttg aatcgtcaca
     1261 tgatttcttt catcagtgta attttcaaat aactgttcaa ttgttaattc attttctata
     1321 ttagccgctt cgataattag ccgcttctga ccgtttactt cttcttgtat tatcttcact
     1381 ttgtcttctg tatgaattcc aagatattcc ataatatact ttggcaatct aaggccatta
     1441 ctatttcccc atttttttat aggtaacatt tccatagtag a
//
LOCUS       DAJSKS010000252         1450 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJSKS010000252 DAJSKS010000000
VERSION     DAJSKS010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.262"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..1382
                     /locus_tag="ON396_002828"
     CDS             <1..1382
                     /locus_tag="ON396_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR3114861.1"
                     /translation="GGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIE
                     VTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGT
                     TVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKV
                     TTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEV
                     DVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTA
                     GNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTV
                     TLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSG
                     KNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLG
                     YKNRKKENE"
BASE COUNT          568 a          229 c          314 g          339 t
ORIGIN      
        1 ttgggggcac agtagatcta acagataatg ttactaactt gccagcgtta ccagaaggaa
       61 caacagtaac agatgttact cccggcggta caattgatac caacacacca ggtaattacg
      121 aaggtgtcat tgaagtaacg tatccagacg gtacgaaaga tacggtgaaa gttccagtag
      181 aagtgacaga caatcgttct gacgccgata aatatgaacc aacagtagaa ggcgaaaaag
      241 tagaaattgg aggcacagta gatttaacag ataatgttac taacttgcca gcgttaccag
      301 aaggaacaac agtaacagat gttactcccg gcggtacaat tgataccaac acaccaggca
      361 attatgaagg tgtcattgaa gtaacgtatc cagacggtac gaaagatacg gtgaaagttc
      421 cagtagaagt gacagacaat cgttctaatg cagataaata caatcctgaa ggacaaaaag
      481 tgactacaga cttaaataaa gagccagatg catctgaggg aattaagaac aaagaagatt
      541 taccaaaagg aactacttat gcttggaaag agaaagtaga tgttagcaca gctggaaata
      601 aaaaaggtac tattgtagtg acatatccag atggatcatc tgatgaagtt gaagtagacg
      661 ttacagtgac agatactcgt tccgatgcag ataaatataa tcctgaagga caaaaagtga
      721 ctacagactt aaataaagag ccagatgcat ctgagggaat taagaacaaa gaagatctac
      781 caaaaggaac tacttatact tggaaagaaa aagtagatgt tagtacagct ggaaacaaaa
      841 aaggtactgt agtagtaaca tatccagatg gatctaaaga agaagtagaa gttactattt
      901 ctgtagtaga taaaaaagca ccgaataaac ctcaagttga tcctattaca gagggtgacc
      961 aaattgttac tggtaaaact gaaccaaatg cagaggtgac agttacatta ccagacggaa
     1021 gtcaacacca tgggacagct gataaaaatg gtaattttac agttaaagtt cctaaattag
     1081 aggcagggac aaaagtgata gtaactgcaa ctgatgaatc tggtaatact agtgaaccta
     1141 ctaatgtagt tgtttcttcc aacaaaaaag acagtggaaa aaacggtagt aaaggtagta
     1201 agactgacaa tcaaggtagc aacagcaatc aagacaaaaa tagaggcaaa agccagtcct
     1261 caaaagtttt tcctaagacg ggagagagtg acagtaatat ctttactatt agcggtggtt
     1321 taatactgtt aggaacgtta ggattattag ggtataaaaa tcgaaaaaaa gagaatgaat
     1381 agaaaatcaa tttcttaagt cttctctgag aagaggaaga atctttttct tcctcttttt
     1441 gcttttttat
//
LOCUS       DAJSKS010000253         1440 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJSKS010000253 DAJSKS010000000
VERSION     DAJSKS010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.263"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            19..1188
                     /locus_tag="ON396_002829"
     CDS             19..1188
                     /locus_tag="ON396_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCR3114862.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          516 a          262 c          233 g          429 t
ORIGIN      
        1 gagactaaat aaactattat gaaaaaagga aaacatgcta aaaaaagaaa atattcattt
       61 ttaatgatcg ttgtcgtgtt gtttagcctt agtgcgttct ctacaggaac tattcttgga
      121 tggcaatttt acaataatga acactctcag atagttgccc atgctcaatc agctcaaaaa
      181 aagttgatac tgactaccaa tgaacaacag ctcgttgaaa aaatcgatca actattaaga
      241 aaaaatgatt atattggctc aatttacgta aagaaaaata atcgcatcat catggaaaaa
      301 ggctatggct atgcgaataa actcacgcaa caaccaaatg atccttctct ttattatcag
      361 attggttcgg ttcaaaaagc attgactact cttttgattt taaaacaagt cgagcgtggg
      421 cacctttctt tagatactaa gctggctcaa ttctatccac aaattgctgg cagccagcaa
      481 attactatcc gtgacttgct gtatatgcgc tcaggcttgc accgaactgc ttctccaacc
      541 ataccgatga ctgatgaaga agtcgttcaa tttgcgattc agcacttaaa atttacagat
      601 tatcagacct ctcattatga ttcattgaac tttaccatat tgactggaat tttgatcaaa
      661 ttaacaaatc aaccgtatga aaaattattg accgatgata tcatcgaacc tttaggatta
      721 cagcatacaa aattttatga ccaagtcaaa aattcatcca atcatgcact atcctatcaa
      781 atgtctaaag aggatgatta ctttaaacca ctgacagaat ctgaaacaga gatccataac
      841 gaattaggga ctggtaatat cagtatgtcc gtctatgatt taaatacgct gtttacacaa
      901 gttttatctg gaaaactagt ttccaaagac ttactcttct cattatggcg tgaaaatgcg
      961 aatggacatc catatagtgg tggggtatat agcggagata attatatcct gtcgcaagga
     1021 aatatcgact cttttgatac atctgctata tttagcaaag attcacagga tgctatcatc
     1081 atggaaagca atataaaagc tgacaaaact atcaaactgc ctactgctga tctaagaaac
     1141 caaatctatg acctgatgga aggaactact aagttggcaa atagctgata tgatgaaaag
     1201 atagcaaata ctacttcttt gaatagcatt tgcttaaaaa aagtactcga ctaaacgtag
     1261 gaaagagtgt gggacaaatc taaaaatcaa tttttgctac agactttaat tccaaataaa
     1321 cagaacagaa agaggtgttc ttctttctgt tctgtccaaa ttattctaag atttctcttt
     1381 accaacatta tttgacaaag ggcctattaa atctaaaata gtatctttag attgctaaaa
//
LOCUS       DAJSKS010000254         1417 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJSKS010000254 DAJSKS010000000
VERSION     DAJSKS010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.264"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(150..1337)
                     /locus_tag="ON396_002830"
     CDS             complement(150..1337)
                     /locus_tag="ON396_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HCR3114863.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAJSKS010000255         1412 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJSKS010000255 DAJSKS010000000
VERSION     DAJSKS010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.265"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            97..363
                     /locus_tag="ON396_002831"
     CDS             97..363
                     /locus_tag="ON396_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCR3114864.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            390..689
                     /locus_tag="ON396_002832"
     CDS             390..689
                     /locus_tag="ON396_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HCR3114865.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            863..>1412
                     /locus_tag="ON396_002833"
     CDS             863..>1412
                     /locus_tag="ON396_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HCR3114866.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
BASE COUNT          514 a          201 c          301 g          396 t
ORIGIN      
        1 caatcttctt taatagaaga ttgggctatt tttatggcaa aggagggaaa agaaatgttt
       61 caaaataaag gataacgtta tgtcacaaag ggagtaatgg acagtttgcc ggtggaatta
      121 caagctttat gttggaatct aatcgatcaa aacgttcaaa agcaatttcc tttagactat
      181 cttcaaattt ttgaattttc tacggaaaaa ggaaatcaaa agttggttca tcgtcaagaa
      241 gaaccggaag aaagaaaaga gtatctgatt tcacctaagc ttcgtttgaa atcagtgtct
      301 caaaaaatat gggccattga ttcgggtgag tatcaaacga tgttattacc agaagaatac
      361 tagtatctaa aagaaaggaa cgaaaggact tgttagaaac ggctatcgaa gtactggaaa
      421 agtgtgcaca gctggtcact gcttctgaag agtggggcta tgaatccgtt acgatggaaa
      481 aagaggaaat tgaaatgggt atgcttcctg aagatgttca tctaccacgt ttagtgatga
      541 cacatcttta tatttattgc gcaccagaag atggaaaaga ctatgtagtt tatttcatta
      601 cagatattac cagccagcga gaatttgttc gtgggttact ggtagaagga cgattggttt
      661 ggagtcagat tggaggaacg gatgaataag atcgattatt tgagaatgat tgcgaaatgt
      721 ttagaagata tccttaccgc gattcatcaa attctgaaga aaaaaggaga aagaatatga
      781 aatattttca actagtattg acacttataa cggcaatggt aattgaagtg cttgaacaaa
      841 aaatagcata ggagaaaaag agatgattga aaaagaatta gtgaccacac aaacagaagt
      901 ttggcgagat cctcatcata cccatcgcta tgttttcaaa cgacaatggg cgatcaaagg
      961 aaaagaagcg gaagataaat tagcagtagt cattactatt cgtccaacag atacagaacc
     1021 atttacgaat gatttgagta tgttattaat cgaaaagaat atccgacaat taggatttac
     1081 tggttttatt gctgtgaatc ttttttcgta cacaaaagcg aaaagtcctc gggtatttct
     1141 tcgaggaaat gatgaacaat ctttggaaat attaacgact gtttttacag aaaagaaaat
     1201 caataccatt atttttgctt gtggttcgat tacagcgacg agtcaagtgg cttatgaaca
     1261 agcaaagact atttacgatc aattgagtgc gaaacaaaag aagatgacca aagtattgat
     1321 aaatgcagaa ggtaagttat cccacccact aagtattcat gttcgtaatg aatggcaatt
     1381 ggcagatagt caattattat ttcaagaaaa at
//
LOCUS       DAJSKS010000256         1411 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJSKS010000256 DAJSKS010000000
VERSION     DAJSKS010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.266"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            92..>1411
                     /locus_tag="ON396_002834"
     CDS             92..>1411
                     /locus_tag="ON396_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114867.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRI"
BASE COUNT          504 a          195 c          263 g          449 t
ORIGIN      
        1 ataatccatc ataaaatcag tataggtgaa aagatgaact ttcatctgca tatcaagtaa
       61 ttggtatgag gcattttttg aggtgtagga tatgaagttg gattacctta tagaagagga
      121 agatagagaa aaactgctgc tagctagata tttagaattt gagaaaaaga attatgtagt
      181 gctgcctaag ttatatgaaa cattaggttt gtctaaattt aaaacaagaa attatatcat
      241 agaattaaat aaagaattta ctgaattcaa aacaaagcca gagatccaaa tttcagaaga
      301 agcagagatc catgtcgaac gtctgaattt gacggatatc aaaaatttaa gaagaatcta
      361 ctttcaacga tcaaaaattg ctcgactgtt aacggagatc gtggattcaa aagtaacgat
      421 cgaaaaattt gcagagaatc attttttaag catatcaaga gcctatgtaa aaagaagaga
      481 attagtctct tttcttgaac agcatcaaat aaagttaaag aaaaacatat tagttgggga
      541 agaaataaac atacggaacg tattatttgg ggtatatttc ggggtcttta atggtttttc
      601 tatgccattt agagaaagcg tcatgaatga agcaaagagt atcgtgcaat attttgaata
      661 tctgttcaat ttaaaactgt ctgcaacaaa acgaatcaaa ttcaatttgc taaccactat
      721 tagtttgttg agggcctatg ggaatcaacc aatcggaaaa cgtttctttt cagatgaata
      781 ctttaagagc tcagaaggaa tgaagatcat caaggattta tcacatattt cttttcttca
      841 tatccctaaa gagcagatca ttgatgagat ttcttatatc atgttgtttt tgatcacgga
      901 agaagaattt gaattagatg gcgcatttgg acttgcggaa gctcattttc atgatatcca
      961 catgattagt caaaaaatgt cagtggaatt gattgatcaa ttaccatttt cgattgaaat
     1021 ctctgaagaa gaaagactat atatacgaaa agaacttcgt atgggtatga tgcatgtcaa
     1081 tcgaaaaaat tggatattcg actttgagat gactagcttt acgtctaaaa agcagctaca
     1141 gtttttcttc gagacgtatc caatattcag tacagcaatc caaaaggtca tacaagagaa
     1201 taaacgctat ttcttgggaa aagatgaacg tttcatgatt cgcttatttt atgattatct
     1261 ctttttattg gtcggtattt gtccggtatc atattttgaa gcaccgattc atgtctgtat
     1321 tgatttttcg caaggatctg cttataccga ttacattatt tctcaagtga aaggatttaa
     1381 aaattacaat attgtgattg agagtcgaat c
//
LOCUS       DAJSKS010000257         1398 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJSKS010000257 DAJSKS010000000
VERSION     DAJSKS010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.267"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            63..1295
                     /locus_tag="ON396_002835"
     CDS             63..1295
                     /locus_tag="ON396_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR3114868.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT          457 a          266 c          308 g          367 t
ORIGIN      
        1 tcataattaa atggtatcat gctaatagtt accagaaaaa atggtgagga ggaattttta
       61 ttatgttatc tgattttaat gaacttctaa aaaaatatgc acgcttaatt gcagaaactg
      121 gcgtagccac agaaaaagga cacacagttg ttttacagat cagcgtggaa caggcacccc
      181 ttgctcgatt gatcacacaa gaagcttata aactaggagc cgctgaggtt atcgttcaat
      241 ggactgacga ccagatccaa agagaatttc ttttacatgc tgcaacggat cgtattgaga
      301 atgtaccaca atctaaaatc gatcaagcgg atgaatggct tgaaaaagga gcaagcagga
      361 tcagcgttgt ttctgccgat cccgatgcat ttgctggtgt agatagccat cgagtagcgt
      421 cttatcaatc agcgaatggc aaagcactaa tgaatttacg caaggcaact caagcaaata
      481 aagtcagttg gactgtcgtg gctgcggctg gtaaacaatg ggcagcaaaa gtcttcccag
      541 aactgccaga agaaaaacaa gttgaggcat tatgggatca gattttcaaa acgactcgtg
      601 tctatgaaga agaccctgtc ttagcatgga agaagcatga tgaaaaatta gcaaaaaaag
      661 cagaagaatt gaatcgagaa caattttctg cccttcacta tactgctccc ggtacagata
      721 tcattatcgg attgcctaaa aaccatcttt gggaaggcgc tggaagctat aatgctcgtg
      781 gagaaaaatt catggcaaat atgccgacgg aagaagtgtt cacagcacct gacagtcatc
      841 gagtagatgg ttacatttca agtacaaagc cactgagtta tgctggcacc atcatctcgg
      901 gtatgaaatt tacttttaaa gatggaaaag ttgttgattt ttctgccgaa caaggagaag
      961 atgtcttagc aaaactgctt gatacagatg aaggtgcgcg tcgtttagga gaagtcgcac
     1021 ttgtacctga cccttcacct atttctcaat ctggcatcat ctttttcaat acattatttg
     1081 atgaaaacgc atcgaatcac ttagcactag gttctgctta tgccttcagt gtgaaaggcg
     1141 gtacagaaat gtccgatgaa gaattagcag aagctggatt gaatagaagt caaacccacg
     1201 tagatttcat ggtaggatct gacaagatgg atatcgacgg aatccgtgaa gatggctcta
     1261 cagtaccgat tttccgaaat ggcgattggg cttgagtata ctttcgcaca catgcaaaaa
     1321 ataatataaa gcatagaata aaggatctaa gctattggct tagatccttt atttcgctaa
     1381 acaaccccac cgtttttt
//
LOCUS       DAJSKS010000258         1391 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJSKS010000258 DAJSKS010000000
VERSION     DAJSKS010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.268"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            6..416
                     /gene="spx"
                     /locus_tag="ON396_002836"
     CDS             6..416
                     /gene="spx"
                     /locus_tag="ON396_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCR3114869.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            430..786
                     /locus_tag="ON396_002837"
     CDS             430..786
                     /locus_tag="ON396_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCR3114870.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          510 a          208 c          259 g          414 t
ORIGIN      
        1 agaaaatgat aaaagtatat acttcgccta gctgtacttc ctgtagaaaa gcaagagcat
       61 ggttgcaagc gaatcaactt gaatttgaag agaagaatat ttttgccgaa ccgttaacag
      121 aaagtgagat caaaaagatc ttggcagcga ctgaaggagg agttcaagat attatttcca
      181 ctcggtcgaa aatttacgaa aagctgggca tagattttaa cgaattaact ttaaagcaaa
      241 tgatcgtctt attcaaagaa tatccttcat tattgagacg acctattttg attgatgaaa
      301 aaagaatact aatcggattt aatgaagatg agatccggtc atttgtccct cgtgaaattc
      361 gatgtgttgc cagaaatctg atctatttac aaaacaataa catcttaaat tcatagggag
      421 ggagccttcg tggaagtgaa agtctatgga tcagcgcact gtgtaacaag tctgaaggcg
      481 aaacaatggt tagaacacca tcaattgcct tatcaatttg ttgacttaga agaacgcgat
      541 ttaagtacag aagaagcaga agaattatgc agttttgaag aagtgcaagc agaacagcta
      601 tttgcgactt ggtctgaggc ttttcacaac attgcgattg acctttcctg tgtaggaaaa
      661 aaacaactga tttttttatg cacacaacat accaatctcc tcagacgtcc tttgattatt
      721 atcgatgaag ttctatttat tggatacaat gagcagttga tggaacagct tatcttaaat
      781 aaatagactt taactgtttt aatcaagtca ctgagcattt catacagttg taacagtcga
      841 acacttagca tcaagcgtgt tatgtgttct ttttttgact ttatttttct actatagaga
      901 catgctcaat ttatgtggtc aaaattcaaa tctaggtggg gatatagagt taaaaatata
      961 ttgacatgtg taaatttaca tgatatattc atttctgttg ttttaagtag caaagcaata
     1021 aaatagtatt ttaaaaaaca tataaaatag ttgttgacat gttagaaatg acatgataag
     1081 ataactgagt tgttaaaaaa gaaccacttg tgaagcttga ttctatcgaa aaaaacttca
     1141 aaaaagttct tgacaaataa caaatgataa gttatgataa atgagttgct gtaagatttc
     1201 gaaaaacgaa atcaaaaaaa cttttaaaaa agagttgaca attacttgtc tctctgatat
     1261 gatataaaag ttgctacggc agccaaaaac gaatcgggga acactcctga tgaagtagac
     1321 ctttgaaaac tgaacaaagt aagacaaacc aaatgtgtag ggcgtcttga ttcaattcaa
     1381 gacaacaaac a
//
LOCUS       DAJSKS010000259         1383 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJSKS010000259 DAJSKS010000000
VERSION     DAJSKS010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.269"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            33..1367
                     /locus_tag="ON396_002838"
     CDS             33..1367
                     /locus_tag="ON396_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114871.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT          488 a          200 c          247 g          448 t
ORIGIN      
        1 gtttttatta tggagaaaag ttttttaaaa agatagatgc tgctttttta tttagttgtt
       61 tatatacttt tgcaacttat cgaacagttg atatctatta tcgatccgca attgcagaag
      121 caatcgctct tacgattatc ccagctgttt tatattatgc ttatggattg atattcaata
      181 gagaaaaaaa agtcatcgct ttatctgtca gtataagtct acttgtgtat actcatgtct
      241 taactacact attgtgtagt gcattgattg cttgtatgat tatagttcgt ttgtttttaa
      301 gcaagcaaat aaaaaacgat ataaaatttg tggtcgtgaa tttagtaaag gctgtttttt
      361 tgacttgtgc attaactagc tatttttggt atcctatgtt gcagcagatg aattatcaag
      421 aaatacacag tcccttcaaa acagatttgc agctagaagc actttctatt tcagatagta
      481 tcattcaagc gttaaataac gatattacaa catacactat ggggttatta ggattggttg
      541 cgttgatttt tcctttattc atactgaaag aaatggagaa aaaagaaaaa atcatctatg
      601 ttggcgtagt ttctacatgg gtatgcgtaa ctaatttatt tccatggttt ttgtttcaaa
      661 aaacaccgat aagcttgctc cagtttcctt ggcgtatctt aggtatccaa atcttttttt
      721 cttcaatgat cataacgctt gtttttatga aaaagactat gaataaaaaa tattcatggt
      781 taatcattgg cgctactgtg gtgtttctac tcgtcacgtc tatagcagca aaagaaaatt
      841 acgcaaaagt tatccaatca aaacacggtc atattgttat caatgaagaa acactcccac
      901 gttatacaac atcagatatg ggaggattat acgattacgc cccaacagaa gcgataaaag
      961 aaaggcctca cttagaaaaa catgaagtaa aagtaggaga atcgtgggaa aaagggaagt
     1021 ataaagctgc agataataac attacttaca cagtggcaag caacaaaaag caaaaagtga
     1081 cagtaccagt atatgcttat ttaggtacac aggtaaaagt aaacggtaaa gtcgtaaatg
     1141 actcttataa aaaacatggc ttagtgaatg tggatgtaaa tgctggagaa aataagatag
     1201 aaattacaac aaaatatact ccaactgctc tcatggcttt atggatcagt attctgactt
     1261 attgtttagt ggtgtattca agttttaaac gatttgcacc taataagttg aaagattctc
     1321 gatatgtgac taaaaaggag ataaacaaga agaatgaaaa taaataaaaa agtagtaata
     1381 ttg
//
LOCUS       DAJSKS010000260         1368 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJSKS010000260 DAJSKS010000000
VERSION     DAJSKS010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.270"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            82..>1368
                     /locus_tag="ON396_002839"
     CDS             82..>1368
                     /locus_tag="ON396_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HCR3114872.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DAJSKS010000261         1361 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJSKS010000261 DAJSKS010000000
VERSION     DAJSKS010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.271"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            14..775
                     /locus_tag="ON396_002840"
     CDS             14..775
                     /locus_tag="ON396_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR3114873.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(890..1330)
                     /gene="fabZ"
                     /locus_tag="ON396_002841"
     CDS             complement(890..1330)
                     /gene="fabZ"
                     /locus_tag="ON396_002841"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR3114874.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          452 a          265 c          259 g          385 t
ORIGIN      
        1 gaaaggaaca gttatgaaga aaaataaagt tattttaagt attagtttag cagcagcatt
       61 gctgtttgct gctggttgtt ctgcaaacaa cacgacttct tctagttcgt ctacagaaag
      121 ttcaaaaagt acatctactg cggaaagcac gactgccagt agcgtcgatt tgaacgcatt
      181 ggaattacca caattgacga aagatgtcgc cgaaaatgaa gatctggtcg aaatggtgac
      241 gaataaaggc acaatcaaaa tcaaattatt tcccgaacac gcaccaaaag cggttgaaaa
      301 ctttatgaca catgcaaaag acggttacta caacggactg actttccatc gcgtcatcaa
      361 agatttcatg atccaaggtg gcgatcctaa aggcgatggc actggagggg aaagcatttg
      421 gggagaagga tttgaaacag agatcaacaa ccacctttat aatataagag gtgcgttatc
      481 tatggctcgc tcacaagatc ctaacagtaa cggcagccag ttctttatcg ttcaaaatac
      541 agatgatatg catgatggtc ttttaaaaga cgactatcct caagcaatca tcgacgctta
      601 taaaaacggc ggttatcctt cactagatgg ccattatact gtcttcggcc aagtgattga
      661 aggaatggat gtcgtggatg ctattgcaaa tatcgaaaca gatagttcag acaaaccaaa
      721 agaagaagta aaaatcgata aaatcaatat actgcaagaa gcaaaacaaa aataaaaaaa
      781 taacgtctga atcctgcata gatgacaacg tatgctagga ttcaggcgtc ttctttttac
      841 ataaaaagca agtaagcttc cttttgtttt gctatatcag gttttatagt caagcatttt
      901 ggttgacgat gaacgtgaac tcgcactcac agactttttt atcatctaca tatgctgcag
      961 ataacgctgt accgactgat cccttcattt tggtgatttc aaaatgacat ttcagcacat
     1021 ctcctggaac aactttttga cggaatttgg ccttattgat cccaccgata taagctcttt
     1081 ttcctagaaa ctcttctgat tttaggataa gtatcgaacc tgcttgtgcc agtgtttcta
     1141 gaatcaacgc acccggcatc actggattgt caggaaagtg accttggaaa aaatcttcat
     1201 tgattgttac attttttgtc gcaacgatct tttttcctgg ttctaaagta tctacataat
     1261 caatataaca aataggataa cgattaggaa tcaattccat tacttcagta gcagataata
     1321 cgtaagacat aattaaactc cttctttcag gcaattgcca t
//
LOCUS       DAJSKS010000262         1304 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJSKS010000262 DAJSKS010000000
VERSION     DAJSKS010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.272"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..565
                     /locus_tag="ON396_002842"
     CDS             <1..565
                     /locus_tag="ON396_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3114875.1"
                     /translation="AQGIVLMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSA
                     IVYLILFLIKASNMYVFLILSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTV
                     YAVYSFARKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIV
                     GLILFFLYPLSKDKVLENCELLKQKHK"
     gene            575..>1304
                     /locus_tag="ON396_002843"
     CDS             575..>1304
                     /locus_tag="ON396_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR3114876.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLS"
BASE COUNT          463 a          141 c          251 g          449 t
ORIGIN      
        1 tgcacaggga atagttttaa tgaattttat caatcctatt ttggttttaa tagttgtttc
       61 gcctttaact ttgtttttaa gtaaaaaata tggaaaaaaa gaactggcat cagtaggaat
      121 gttcttttca gcaatagtat atttgatctt atttttgata aaagctagta atatgtatgt
      181 attcttaatt cttagttcgg ttggctatat gggtttgggt gtgtttaata ctgttatttg
      241 ggcaaatatt actgatgtta ttgatgatca agaagtaaag tctgatcaaa gagaagatgg
      301 gaccgtatat gcggtatatt cttttgctag aaaaataggt caagctctag ctggaggcgt
      361 cggaggttgg actctttcta ttataggcta tgactcttta gcaaatgtac aaacgggaga
      421 tgttttagaa aagttatata acgcttcaac attaattcca tcaatttgtt ttctgattgt
      481 aggattaatt ttatttttcc tttatccatt atctaaagat aaagtcttag aaaattgcga
      541 gcttttaaaa caaaaacata aataggagag aaatatgaat aattggatat tttgtattga
      601 ttcagatggc tgtgttatgg atacgatgac ttataaacac aatctctttt tcggaccaat
      661 tgctgcagag atttttgatg ttcatcatag agaaaaattc ctacaggatt ggaataatat
      721 taatttatat tcagagactc gtggaataaa cagattttta ggtttagtta aaacattaga
      781 aaaaaatgat gtaacatcga ttaatcgatt aaagcactgg gtatctctaa caaaagaatt
      841 gtctaatcca tctttgaaga gggaaataga atattctcag tcagaggatt tattaaaagc
      901 tttaaaatgg tcggaagaag tcaatcaaca gattagtcaa gcaaaaggat tagataaacc
      961 ctttttaagt gctagagata ctataaaagc tctgaaaaaa tatgggaaga taattattgt
     1021 aagctcagct aataaggaag ctgttcaaga agaatgggaa agacatgaat tattatcatt
     1081 agttgatgaa ctgtgttgtc aagataaagg aaaaaaagaa gatattattc gaagtgtaat
     1141 tgaaaatggt tgtgatttgg ataagatatt aatgattggt gattctcctg gtgatctaga
     1201 agctgcaaat aaaaataaag ttttctttta tcctattttg gtcaataaag aaaaagaatc
     1261 atgggaaaac ttaagaactg atgttttatt taaatgttta tcgg
//
LOCUS       DAJSKS010000263         1294 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJSKS010000263 DAJSKS010000000
VERSION     DAJSKS010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.273"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..1244
                     /locus_tag="ON396_002844"
     CDS             <1..1244
                     /locus_tag="ON396_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR3114877.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAJSKS010000264         1268 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJSKS010000264 DAJSKS010000000
VERSION     DAJSKS010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.274"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            36..509
                     /locus_tag="ON396_002845"
     CDS             36..509
                     /locus_tag="ON396_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HCR3114878.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            518..745
                     /locus_tag="ON396_002846"
     CDS             518..745
                     /locus_tag="ON396_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3114879.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          403 a          198 c          211 g          456 t
ORIGIN      
        1 atattttagc tatcaaagat aaaagaggga taaaaatgaa atttaaaaca ttgctattaa
       61 aatttgtcgt tttaggtata agtgccgtat ttattttgtt cggcgtactt ttttatactc
      121 agatcgctac aagcgaaaaa ggattcaatt ttgattggca gaccacatta tttgtttctg
      181 tcatttactt tgcccttctc ctagggatcg ttattgctta tttcttattg aaactattga
      241 aaaaaatcga agataaccaa gctttttcca ctcaaagctt aaacatacta aaaaaaatcg
      301 aacgaaccat tttgtttatt ggtttcgcct tttttggctt acttcctaaa tttatgaaag
      361 tagcggattt agacgatgct ccaggtttga tgttggtagg aatcgctgtc gtattcattc
      421 cttttgcagt atacacatta acaagcgtgc tggaaaaact gcttgaacat gcgattttac
      481 ttaagacaga tcatgatttg acagtgtgag gtgcgacatg gcaattatcg ttaatttaga
      541 tgtagtatta gctcaaaaaa aaatgagtgt gactgaattg agtgagaaag tgggcattac
      601 gatggcaaac atctctattc taaaaaatgg aaaagcaaaa gccatccgtt tttccactct
      661 cgaaaaaatc tgccaagttt tggagtgtca accaggtgat ttgttgacct ataaaaatga
      721 ctcgaatact ataccagaag aataaaataa agtagaacaa acagtaaata aaaaataatc
      781 tgaatgatag atgtgtgaga cttttagaag ttatttacgt ctattattca gattttttat
      841 ttcgattgaa taccgtttta tctataaata agatatgtac tgactcatac aaagggggcc
      901 ttttcttctc catgtactat cacttttcct gatacaactg gtttcctgtt acgttgtagt
      961 tggatctgct tttagtataa tactttattc attccaccag actgatagca gtttgagcag
     1021 tctaaataat catgtagtat tacaggccat tctgtttcat accttgaagt gcttaaaact
     1081 tcagctaatg gcccattgtg ttaactctta ctccattctt ttttagtaag ctcccatata
     1141 tactgtaaag ccttctatat attatgtcat gttgcttttt aacatttact atgtagtttt
     1201 gataaaatga tttgaataac agtgatggac aattcttttt tatttttaac attattttga
     1261 taataatg
//
LOCUS       DAJSKS010000265         1249 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJSKS010000265 DAJSKS010000000
VERSION     DAJSKS010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.275"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..>1249
                     /locus_tag="ON396_002847"
     rRNA            <1..>1249
                     /locus_tag="ON396_002847"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          327 a          282 c          384 g          256 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccc
//
LOCUS       DAJSKS010000266         1241 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJSKS010000266 DAJSKS010000000
VERSION     DAJSKS010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.276"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            109..1194
                     /locus_tag="ON396_002848"
     CDS             109..1194
                     /locus_tag="ON396_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR3114880.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
BASE COUNT          465 a          128 c          248 g          400 t
ORIGIN      
        1 acctaaacaa aaatttgttt atagattaaa atcaacaatt ggacaaaaaa aatttaacag
       61 gttacttgca ctgaaaaaat ttttacactt ataaaggaga aaagtacttt gaagaaaatt
      121 tgtttcatta gtggtgtaat ttctcgttct gggggaactg aaagagtagg gagtattatt
      181 gccaatagct tgtctgattt aggttatgat gtcactattt taagcttttg gaatagtgga
      241 gagccttatt ttgggttaga taagacgatt aaggttgatt atatgttaaa tcctaaaacg
      301 gagggaaaat tatatagaac ttatttatat ccggtttgga aattgcacaa gtatatatgc
      361 aaaaatgaat ttgatatagt aattgatatt gatactttgt tgtctttgta tactacaagg
      421 gcaattaaag gaacaaagtg caaattgatt tcttgggaac attttaatta ctggacaatg
      481 gaaatgctga aagaaaagaa gagattcaag gctaaaaaat tgataaaaaa atatgcaaag
      541 aggcttgtgg ttctcacaga agaagatcgt caaaaacata ttgatgaata tagtttatca
      601 gaagattttg ttataactat gccaaatccg tgtatatcta atatcgagta cgaatatcat
      661 tatgataaaa agagatttct tgcagtaggg cgattagtgg aagagaaaaa ttttgctgac
      721 atgataagtg cttggaaaat tgtagaaaaa aaagtagatg attgggatct tatcattgta
      781 ggaaaaggtg aattagaaaa agatcttcgc cagcagataa aagatttaaa tctgcaaaga
      841 gtaactatag cagggcattc agaccatgtg gaggaatatt ataaatctgc atcttgttat
      901 gtgcttagtt ctgtttatga aggatttcct atggttatat tagaagcaca gtcttatgga
      961 ttaccagtta tttcttatga ttgtaaaact ggtccgagag atttggtaca tcataatttt
     1021 aatgggatgt tggttgaaga taagaatata gaccaattag ctaagagcat gataatgttt
     1081 actaagaata ctgatttagc aatgagaatg tcactgaacg catataataa tgtacaaaaa
     1141 tttaatttga aagaaattac aaaacaatgg gttgctttga ttgaaaatgt atagtttttt
     1201 gtggttgtcc aaacatgaaa attagcgtaa ttaatcaaag a
//
LOCUS       DAJSKS010000267         1238 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJSKS010000267 DAJSKS010000000
VERSION     DAJSKS010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.277"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(240..1100)
                     /locus_tag="ON396_002849"
     CDS             complement(240..1100)
                     /locus_tag="ON396_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3114881.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT          372 a          237 c          179 g          450 t
ORIGIN      
        1 acaaacaaca catttttgtg tgttgcttat ttccctctaa ctatcgtttt ctatttgatg
       61 gttaacctac ttaaaataaa tactgcaacg acaaacggag atcaagcatg ctttgtaacg
      121 tattttacga taattacgac tttattttat tactctatct tgctgccata cccgtctatc
      181 cctaatcatc tttggtgaat tttttcaaag ttagcataac aaaaataagt attttttcct
      241 tagttaatca atagtatcat ttttttcata tgatttaatt catcaattaa ttttttgttt
      301 ttattaattt tagcaaaagc tagtgcatct tgaatcaatt cgagcacttc tgccttcgac
      361 ttatttttag cctgtgcgtt cttcgctaat tgggcagcag ctcgtgctaa gtaataagtc
      421 aaatggttat ccgaacaaat atcgattgcg taatttagta gtgcatcact tgtttccaaa
      481 tcaccgtctt gcgcaaaaaa aacaccacaa tgaaagacga tatttaacac ccgccagata
      541 tcttctgttg tacgaatcgg atacgtataa attttttcaa acactcgatt gaaataaact
      601 tctgcttttt ccaactcttt ttctctagca tagaccattc caatacctgt atatgccaat
      661 aaacgaaaca acgtgttggc tgtttctgcc atcaacaact gatcaaaatg gaacaacaca
      721 tccgtaattg gtgcattcgt aaaaatcatt tcaaatcctt ttagatagta gtaacgtgca
      781 atcagctcag actcgtcaat taccttggga tcaatggctg ctaaaatctg tgtcaattgt
      841 tcatattcac tcgtaatcag tgaaaactct gctttttcta gctgttcatc aagttgtgta
      901 ttgatcacgt tcatcgaaga aaaaagttca actaacggaa gattgattcg attacataat
      961 ttgattaaaa tttttaacga agggacttga ccattgttct caaatttact taatgttgct
     1021 tgtgtacaaa ttccttcaga cacttctttt tgagataagt tggcagcctt tcttgccgta
     1081 ataaattttt caatgttcat tattttctct ccaatcagac ctcacacgaa aatcatttct
     1141 gtctcgattt tagcatgttt tctttgggaa tgctttaggt tctgttgcaa agttttaaat
     1201 aaagaataaa gtcccttacg gtatctatga tttaagct
//
LOCUS       DAJSKS010000268         1205 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJSKS010000268 DAJSKS010000000
VERSION     DAJSKS010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.278"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(495..860)
                     /locus_tag="ON396_002850"
     CDS             complement(495..860)
                     /locus_tag="ON396_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114882.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(936..1181)
                     /locus_tag="ON396_002851"
     CDS             complement(936..1181)
                     /locus_tag="ON396_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3114883.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
BASE COUNT          408 a          215 c          233 g          349 t
ORIGIN      
        1 tgataaaaaa actattttta ctatcaatat taacgctagc aatggcaggt tgttctgact
       61 ctggcttagc aactgaactg atcacaaaat caactgaagc cacgacagag gagttaaaat
      121 gtggaaacga tcacagtgac tacagaggct taaatcgacg atgcatggga atattaggca
      181 gaagcaattg taagcatcga tgtagtaatc gaagaaggtg tagaagaagc aacagaaaaa
      241 tcaaaagaaa gatcaaaaga agcaatctgt gctgtgcatc gtatacagac gtcgaagatg
      301 cggtactttt aaaccacgtc aggagatgtt catttatgtc tagggaagtt ggcgatgaac
      361 caaaactaga gtacgctttt agttttgcac gtgaggatga agataaccct aaaacttacg
      421 acattttagc ttgatctaag atggttaaag ctttttaatg aagtaagagg agatagaagg
      481 cttttctagt tatattaggt aagataaatt cctttttgct ccaaatttgc taaacattct
      541 gctagataaa cttcatcgtg ttccggataa atcggcacgt tgcgctctac agtaggtaaa
      601 tcaaaaaatg cttcgcatga tttgccacga tacataggat ttttccactc tggtgctggg
      661 ctgtaaccac gtttttccat ttcatccatg acaagctgat gatattggaa taatctataa
      721 ggagaatagt gaaaaacata atccacagta gcatgtttct ttccccagcc atttcctctt
      781 aatgcacagc attcccgatg ttgtcctaaa agctgttgac gaggaagctt tgaaatcaat
      841 ttttcatgcc ataatcgcat agaaatgtct cctttatata tagattgtta accttagacg
      901 ttaaatgaaa aaaccagcac caaggctgga aaaaatcact tcagtttgct tgctgtttct
      961 gagcgaccaa ttaggtaatc aacggaaata tcgtaaaagt cagcgatata gattaattca
     1021 atgatgcttg gaatttcttc tccttcttcg tacttacgat atttgaataa aggaatactt
     1081 aacgcgctag aaacagctac ttgagataaa gatcggccag ttcttaagaa attcagacga
     1141 caacgtaaag tatccagtaa aatagcttct tcgatactca tttaatcacc tttcattata
     1201 gtaga
//
LOCUS       DAJSKS010000269         1203 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJSKS010000269 DAJSKS010000000
VERSION     DAJSKS010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.279"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            566..724
                     /locus_tag="ON396_002852"
     CDS             566..724
                     /locus_tag="ON396_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114884.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            796..>1203
                     /locus_tag="ON396_002853"
     CDS             796..>1203
                     /locus_tag="ON396_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3114885.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELT"
BASE COUNT          466 a          169 c          198 g          370 t
ORIGIN      
        1 tttgaaacta cgcgtatagt aagtagcttt caaaatatat aaaaaaggag cgttctcaaa
       61 tgagaacatg ctcctaaaac tactttattg gcgtatggaa aaattatact tgaagtggat
      121 agcgggttga ataagttgat aagcatcaaa acatttataa aaattattca agactatgtc
      181 tgttccatat acaaatatga aaatcagtaa actccattta acttttttcg tagtgtgtct
      241 ttttatacat gtttatcgtt attaatagtg aataggaaaa aaactatttt tgatcattga
      301 caattaaata caatttttga ataacgatcc ctgctattcg agctagaaac tcaagtacgc
      361 catgacctga ttagcgacaa atacttagag atttcttttt gttctgggaa aacaaaatgc
      421 catgacaata gcagcaaata atgatacttc cgtaaggctc gccgtaaact ggggagaggt
      481 gaaattccta tgtgcttacc caaagcaatt caaaaatttc ataactacta ttttaggaga
      541 tgattttata gaatccagag tctacctgaa taaaaaacaa acctgtgcat ttctaggaat
      601 cgataataat actctagata agtggattcg tatatataat ttcccagtaa taagagttgg
      661 tggtgtagtt cgctttgacg tagaagatat taaagacttt atgaatacat ttaaaataac
      721 ttgaaatttt cttctcgccg catatactaa aattagtgaa atatactgcc ttactttttt
      781 taaggagtga ggattttgcc tacaaagctt tcaaatggga aatataaaac taatctgaga
      841 tatccaaaaa ggtttaaaga aataacagga attgcatcag aaaagtatca aaaaacattt
      901 ccaaataggc aattggcgat taaagccgaa aatgatatga agaagaaaat agaaaaagtt
      961 ttgagagagg agaatgcaaa ctcattagaa ttaaaaggaa aaataacgtt taaaaaattt
     1021 tacgaatcta aatggcttcc tagatatgaa ttaggtcaaa caattagatc aaacaggcct
     1081 cctagtgata taaccattag taatacaaaa gatatatttc ggttgcatat tttacctatg
     1141 tttggtgaat acgcaatgaa ttatctaaat atcaatactg agataatatc agatgaatta
     1201 acc
//
LOCUS       DAJSKS010000270         1195 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJSKS010000270 DAJSKS010000000
VERSION     DAJSKS010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.280"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            3..341
                     /locus_tag="ON396_002854"
     CDS             3..341
                     /locus_tag="ON396_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3114886.1"
                     /translation="MNGIEDENLRRLINSLVIELYKYQAESERKKIKERQAQGIEIAK
                     KKGKFKGRQLKFKKNDPRLKHAFDLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVD
                     QRKNNEKRDS"
     gene            433..1138
                     /locus_tag="ON396_002855"
                     /pseudo
     CDS             433..1138
                     /locus_tag="ON396_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320596.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
BASE COUNT          453 a          190 c          244 g          308 t
ORIGIN      
        1 caatgaatgg tattgaagat gaaaatttaa ggcgtttgat taatagcctt gtcattgaat
       61 tgtacaagta tcaagcagaa tcagaacgaa aaaaaattaa ggaacgtcag gcacaaggaa
      121 tcgaaattgc taagaaaaaa ggcaaattca aaggtcggca actgaaattt aaaaaaaatg
      181 atccacgttt aaagcatgct ttcgatttgt ttttgaatgg tttatccgat aaagaagttg
      241 aagaacaaac tgggatcaat cgccgaacgt ttagaaggta tcgagcaaga tataacgtga
      301 cagtagatca aagaaaaaac aatgaaaaga gggatagtta atgagtacgg ttattttagc
      361 tgaaaaacaa gccaggcatt agcctacgca agtgctttaa aacaaagcac caaaaaagac
      421 ggttattttg agatcaaaga cccactattt acagatgaaa cgtttatcac ctttgatttt
      481 gggcatttag tggaattagc agaaccaggt cattatgacg aaaagtggca aaattggaaa
      541 cttgaatctt tgccgatttt tcctgatcga tacgattttg aagttgcaaa agataaggga
      601 aagcagttta aaattgttgc agaacttctc aaaaaggcaa atacaattat tgttgcaaca
      661 gatagcgaca gagaaggtga aaatatcgcc tggtcgatta tccataaagc aaatgccttt
      721 tcaaaagata aaacatttaa aagactatgg atcaatagct tagaaaaaga tgtgatccga
      781 agcggttttc aaaatttgca accaggaatg aattactatc ccttttatca agaagcgcaa
      841 acacgccaaa ttgccgattg gttgatcggc atgaacgcaa gccctttgta tacgttaaat
      901 ttacaacaga agggcgtaca aggtacattt tcactaggac gtgttcaaac gcccacctta
      961 taccttattt ttcagcgcca ggaagctagt ataaaagtaa aacaagggtc atttaagggc
     1021 gttctaagcc ccacacagcg ttttaaaacc caagaggagc ttttttgctt ttgtttcttc
     1081 taaacaagct aaaataggca atcaagaggg gataattgct gatgttcaaa ccaaagagat
     1141 ggataggcta caattaacta gacagaaaaa ttaaggtgtg tagactagaa gaaaa
//
LOCUS       DAJSKS010000271         1175 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJSKS010000271 DAJSKS010000000
VERSION     DAJSKS010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.281"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(200..1015)
                     /gene="thiD"
                     /locus_tag="ON396_002856"
     CDS             complement(200..1015)
                     /gene="thiD"
                     /locus_tag="ON396_002856"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR3114887.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          310 a          257 c          195 g          413 t
ORIGIN      
        1 caaacatgag aagctgtttt ttgaacttgt tccttgtgac tcggagctgg acggcaggct
       61 cacgacctct ttcttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
      121 gttaaaaaca taagaagctg tttttacttg gctgttctta ttacttgatg cagggcaact
      181 ttttcacaac ttctttcttt tactctaatt caccagctgc ccaatgattg atggggccat
      241 atttgtgccc tacatttatt tcttcacgaa ttgcacggtt tacatagtct tttgccaatc
      301 ggatcgcttg ttcaactgtt tttcctttcg ctaactcagc tgcaatacat gcagatagcg
      361 aatcgccagt tccgttgatc cggtcggtag gataaaatgg tgcatttaac caaaatgatt
      421 ttcctgattc gaacaatacg tagtcattga ctgtttcttg ctgcgcattt ttagcatgat
      481 gtcctttgat cataacgttt tttgcaccca tatcttgcag ttcttttgct gcttgcacaa
      541 aatcttcttc agctgctaat gtccgtccag tcagcttttc tgcttcataa aaattaggtg
      601 tcagtacttc ggccaaaggg acaagctttt ctttcaagct ttgcaaagca gattcttcta
      661 gtaaaagatt tccatgtttc gttacgatta cgggatccac gactaatggt ccaaaatcat
      721 atttttggta attttttaca actgtctcaa tcaactttga atctgctagc atacctgttt
      781 tagctgcacg tatttgaaag tccttcgcaa tcagttcaaa ttctttgtcg ataaaatctg
      841 ttggcaatgt cacacttgct ccgattccgt aagaattacc tgcaacacat gcggtcatga
      901 cagaaacacc gtaaacattt cttgtaaaga atgtatgcat atctgcttgc atccctgcgc
      961 ttccatcgct atctgatcct gcgatcgtca ttgcttgtac atactgatct tccatcaaat
     1021 cgattcctcc atttttataa aaagattttt cataatactg ttactgtttc tatcatatgt
     1081 ttttcaaagt atccctcagt aaatatacca ctaccttttc ttggtaatca aatttatttg
     1141 aaaaaacatt tgcaataaat cagaaaatag tggtc
//
LOCUS       DAJSKS010000272         1137 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJSKS010000272 DAJSKS010000000
VERSION     DAJSKS010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.283"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..355
                     /locus_tag="ON396_002857"
     CDS             <1..355
                     /locus_tag="ON396_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114888.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="ON396_002858"
                     /pseudo
     CDS             615..910
                     /locus_tag="ON396_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            985..>1137
                     /locus_tag="ON396_002859"
     CDS             985..>1137
                     /locus_tag="ON396_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR3114889.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          431 a          135 c          237 g          334 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag
      961 aaaaaagaca gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac
     1021 gtcattattg tcgctgttgg ttactacctg cgttacaatc taagctatcg tgaagttcag
     1081 gaattgttat atgatcgtgg aataaatgtt tgtcatacta cgatttatcg ttgggta
//
LOCUS       DAJSKS010000273         1126 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJSKS010000273 DAJSKS010000000
VERSION     DAJSKS010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.284"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            82..>1126
                     /locus_tag="ON396_002860"
     CDS             82..>1126
                     /locus_tag="ON396_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HCR3114890.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKD"
BASE COUNT          377 a          207 c          219 g          323 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
     1081 tatccctatt ttcatgaatt agttgaagcc tttcgagaca aagacc
//
LOCUS       DAJSKS010000274         1114 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJSKS010000274 DAJSKS010000000
VERSION     DAJSKS010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.285"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..75
                     /locus_tag="ON396_002861"
     CDS             <1..75
                     /locus_tag="ON396_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCR3114891.1"
                     /translation="EFLFPKFLEDSSELQDIYNYLYRR"
     gene            80..1027
                     /locus_tag="ON396_002862"
     CDS             80..1027
                     /locus_tag="ON396_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR3114892.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          356 a          170 c          237 g          351 t
ORIGIN      
        1 gaatttttgt ttcctaaatt tttagaagat tcttcggagc ttcaagatat ctataattat
       61 ttatatcgga ggtaaaagga tgagctgtta tcttatcacc ggtggggctg gcttcattgg
      121 ttctacctta gccaatcatt taggagaaga acatgtggta atcgtcgtcg atgacttatc
      181 aatgggaaaa gtagaaaatc ttgatatgac gaaaaatatc acttttattg agggagatgt
      241 cgctgagaaa tccttgatgg aagagatcat gaaagctaat aaatttgatt atatctttca
      301 tttagcagca gttgctagtg tggcagattc agttgcccga ccagtagaaa cacatcgtgt
      361 gaattttgaa agtgtattaa tgattttaga attgatccgc aagtatcaac ctgatttaaa
      421 acggatcgtg tttagttctt cagcggctgt ttatggggat gaaccgactt taccaaaaaa
      481 agaagagtcc gtgattcgcc cattgacgcc ttatgcggtt gataaattcg ctgcagaaca
      541 atatgtgcta gattattgtc atttatatga tgtacccggt agcgcagttc gttttttcaa
      601 tgtctatggt ccaaatcaaa atccgaattc tccatacagt ggtgtgattt ctattctcgt
      661 agatcgctat aagaaacaaa tcgtaggaga agcaacttca tttacattat ttggtgatgg
      721 aagccagtct agagactttg tttatataga tgatgtaatc caagcattac ttttagttgc
      781 gaatgaggaa gctgcactgg ggaaacaatt caatgttgga acgggtaaag caaccacact
      841 tttggaattg attcatgcaa tcaatgaaat tctcggtgta gaattgacac ttgaatacaa
      901 agaagagcgt tcgggtgata tccatgactc tctagcagat attactaaga tccagtcaat
      961 tggctatcaa ccgaaatatg gtattttaag tggaatgact aattatttga aaactgaaat
     1021 caattaaaac atactaagat aagattagag ggtaaattca ggctctttgt caataaggac
     1081 tgatgagttt tgtaaaatca aatctgggat agaa
//
LOCUS       DAJSKS010000275         1106 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJSKS010000275 DAJSKS010000000
VERSION     DAJSKS010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.286"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..214)
                     /locus_tag="ON396_002863"
     CDS             complement(<1..214)
                     /locus_tag="ON396_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114893.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHIN"
     gene            complement(231..>1106)
                     /locus_tag="ON396_002864"
     CDS             complement(231..>1106)
                     /locus_tag="ON396_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HCR3114894.1"
                     /translation="RLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYT
                     ENLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIP
                     SVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKV
                     RDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRES
                     FKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSP
                     KQYQLGLVDNFNNIS"
BASE COUNT          380 a          191 c          127 g          408 t
ORIGIN      
        1 cattaatatg actgatagat tctctagctt cttcacataa actttttact ttattaatta
       61 ctactttgtc atattcattt aacacactat tattaccatt acttagacgc tcagattcta
      121 cgaaacaact caaatcagaa atttcccaaa aaaatttttt gttaaaatcc aatttcctca
      181 actcctccaa actacgtaaa tgatatagtc tcataataaa ataaaacctc tcagctaatg
      241 ttgttgaaat tgtccactaa acctaattga tattgcttag gagatacacc tgcgtacttt
      301 ttgaatactc tcgaaaagtg tgaagaatct gaaaatccta aacctaaaga aacttcactg
      361 atagtatggt ttgatttcaa cattatttta gccgtagaaa tttttcgctt agagatataa
      421 ttataaatcg taatatgcat ttcattctta aagctttctc ttaatttagt ttcacttaca
      481 gaaaaataat tagctatttc ctttacagta attcttttat agatatttaa gccgatgaat
      541 tgaacgacag aagaaattaa gggagaaaga tgctctatct tagtttttcc gatttcctta
      601 gtataataaa caatcccaga atccctcact tttaagacct caggtaacga tacagctaat
      661 tcgttttctt gtatcaaagc atctctactt tgatacgctt ctattatatc cattcctaat
      721 gttatagacg tttgtgctaa cttttcaaaa acaatgatag aataattttt ttcagaccga
      781 atcgtatctc cactaacact tggaattatt ccattactca gattaaaaac catgttttct
      841 aaatttcgaa tatctccatt tttaacatat tccagtattt tattttcata aagaaacaga
      901 tatatttctg gatcgtaatt ttgtgataac agattatcta ttttgatttg ctcgatagtt
      961 ttacttagat tttctgtata ttcaattaac ggttcatgga atagatcttc tatttttcca
     1021 gtaaaaaaat aatgaactaa aatcagaata tctcgaatgt ctcctaaaga atacaaagtc
     1081 aaattttgca atgaattata gagtcg
//
LOCUS       DAJSKS010000276         1102 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJSKS010000276 DAJSKS010000000
VERSION     DAJSKS010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.287"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            191..445
                     /locus_tag="ON396_002865"
     CDS             191..445
                     /locus_tag="ON396_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR3114895.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ON396_002866"
     CDS             446..763
                     /locus_tag="ON396_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR3114896.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          389 a          171 c          222 g          320 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat ac
//
LOCUS       DAJSKS010000277         1094 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJSKS010000277 DAJSKS010000000
VERSION     DAJSKS010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.288"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            28..1068
                     /locus_tag="ON396_002867"
     CDS             28..1068
                     /locus_tag="ON396_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR3114897.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          449 a          166 c          187 g          292 t
ORIGIN      
        1 aaacaacgtg aaagtagagg atctacaatg actgatttta aattttttaa ggcggataga
       61 gtttacaacg aattatttta tcaatttcca aaagtcttta ttgtttctga cgaatacaaa
      121 aaaatgaaag attcaactaa gattgcctat atgcttttaa aagcaagatt agagatcgca
      181 atcagcaaac ggcaaatcga tgaagaaggt aatgtttatt ttacttatac gacaaatgaa
      241 ctatgtagag tattaaactg ccaaaaacaa aaagcgatag caatcaaaaa agagttggaa
      301 tcctttggtt tattattaca aaagcagatg ggatttaaca aacagttagg gaaaaataat
      361 cctaatagac tatatctagc agaattaaaa gtctcagaaa atgatatcta cttactcgaa
      421 aaatttgata gagagaatag ggaaaacgtt gataaatcag agggtatgaa aatcataccc
      481 accctcgacg aaaaatcaga cgctgaatcc cttggggctc aagagggtat gaaaatcata
      541 ccgtgccaaa acgttgataa atcagagggt atgaaaatca taccagaact taataataat
      601 atattagaca ctaatagaca caatatagac actgaaaaag accgcctaca agatcaattg
      661 ttgttagaca attttgagac aattatgaca aacgacagca ttgctacgtt tgtccctgaa
      721 cgagtattaa atttgataaa aacattttct tcaagttaca gtgaagctca aaaaaccgtc
      781 cagactattc ataatgcaaa gaaaaaagct gaaatagaaa gtgatatttc gatagttttt
      841 gaagaactcg atagttatta tgtcaatgca gaacaagaat tatacacgac actgttaaaa
      901 gcctatcaaa aattaaaaac cgaaaaagtc gaaaatatcc agaacctgat ttttgtctat
      961 gtaaaaaatt ggtttatcga aaaaccaata gctgctaaag tatcaagtga aaaacgtttg
     1021 aattatgcaa gctccccaag cactattacg aaagactggt tagagtgaaa ctttactgac
     1081 aaagtgattg ttgc
//
LOCUS       DAJSKS010000278         1094 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJSKS010000278 DAJSKS010000000
VERSION     DAJSKS010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.289"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(22..531)
                     /locus_tag="ON396_002868"
     CDS             complement(22..531)
                     /locus_tag="ON396_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114898.1"
                     /translation="MKKVLFAVSLVVNIALAGGLFYTITEMNKLDDQRESLAISNEQY
                     KRKLDEASSTNVDTQEAVESSTIVYSNESSETLTNSERSTAPATETVSQKATITIGSK
                     VFTFIVNGLTIEHDSSRTGKMTELSHEFPVIVTTPSATLNGILVQDNNDNNCYIMVDG
                     QTLYRGTYQ"
     gene            647..1094
                     /locus_tag="ON396_002869"
                     /pseudo
     CDS             647..1094
                     /locus_tag="ON396_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          352 a          197 c          197 g          348 t
ORIGIN      
        1 agtcatatta agactcaaaa tttattggta tgttcctctg tatagagttt gaccgtctac
       61 cattatatag caattattat cattgttgtc ttgcaccaat ataccgttaa gggtagcact
      121 aggagtagta acaatcactg gaaactcatg agaaagctcg gtcatttttc ctgttctgct
      181 agaatcgtgt tctattgtca agccatttac tataaacgtg aatactttac ttcctattgt
      241 tatagtagct ttttgagaaa cagtttctgt agctggagct gtgcttcttt ccgaatttgt
      301 tagggtttca ctggattcat tgctataaac aattgtagag gattctacgg cttcttgagt
      361 gtccacgttg gtgctgctag cttcatctag cttccttttg tattgttcgt tactgattgc
      421 taagctttcc cgttggtcat caagcttatt catttctgta atggtataaa ataatcctcc
      481 tgctagtgct atattaacaa ctaaactaac cgcaaataat acctttttca ttcttccttc
      541 tcttctttct ctttttgtaa ttccttactt ttggataggc tacaattaac tagacagaaa
      601 aattaaggtg tgtagactag aagaaaacat accaggagga atttttatgt ctaagagaac
      661 acgaagaact ttttcacaag aattcaagca acaaatcgtc aatctttact tagatggaaa
      721 gccacgtgta gaaatcattc gagaatatga actaacggct tcagcatttg acaaatgggt
      781 aaagcaatct aaaacgagtg gttcattcaa agaaaaagat aatcttacgc ctgaacaaaa
      841 agaattgtta gaactacgta aaagaaacca gcaattagaa atggaaaatg atattttaaa
      901 gcaagcagcg ctgatattcg gacgaagaga caagtaatcg atgcgaataa gcatctttac
      961 cctatatcag cgatgtgcag aatattaggt ctatcacgtc agtcctatta ttattaatca
     1021 aaaccaaaga aagacgaatc agaacttgaa gaagtagtcg ctgaagaatt tatccgcagc
     1081 cgaaaggcct acgg
//
LOCUS       DAJSKS010000279         1073 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJSKS010000279 DAJSKS010000000
VERSION     DAJSKS010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.290"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..153)
                     /locus_tag="ON396_002870"
     CDS             complement(<1..153)
                     /locus_tag="ON396_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR3114899.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(475..696)
                     /locus_tag="ON396_002871"
     CDS             complement(475..696)
                     /locus_tag="ON396_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114900.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(794..1039)
                     /locus_tag="ON396_002872"
     CDS             complement(794..1039)
                     /locus_tag="ON396_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114901.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
BASE COUNT          326 a          208 c          141 g          398 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
      241 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
      301 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat
      361 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
      421 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
      481 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
      541 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
      601 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
      661 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
      721 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
      781 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
      841 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
      901 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
      961 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     1021 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat cta
//
LOCUS       DAJSKS010000280         1060 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJSKS010000280 DAJSKS010000000
VERSION     DAJSKS010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.291"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            23..871
                     /locus_tag="ON396_002873"
     CDS             23..871
                     /locus_tag="ON396_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR3114902.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT          363 a          191 c          185 g          321 t
ORIGIN      
        1 attatgaaaa tgaggaattc tcatgagcgt ctccataaga ctacaaatca aaacaatgta
       61 caacgacttc agcccaaaag aacaggctat cgccgattat attttagaaa atccaagcaa
      121 agtaagtcat agtcctatta gcaatttagc aagtgaactg ggcattgctg actctacttt
      181 ttttcaattt acaaaaaaac ttggttataa tggatttaaa gattttaaaa tggccatgtt
      241 gatccaagaa aatgatctat ctgctatttc tatccacgaa aatattcaga aaacagatac
      301 tgagctcact atggcgcaaa aggtgttcga gtctagtatt tcaactttaa atgacacaaa
      361 aaagttgtta caacaaaagg atctaaaaaa agcggctact attatcaatg aatctaaacg
      421 tgtctatttt ttcggtgttg gcggttcaga gattgtagca actgacgctt atcataaatt
      481 tttgcgttcc cctatcgcaa cttctcacag cacggattac catattcaat tgatggaagc
      541 ttctttattg acggaaaaag actgtgctgt tttgatttct cacacagggc aatcaaaaga
      601 aacaatccat attgctgaaa ccgtaagaaa aactggagca aaaacaatcg ttattactag
      661 ccaagctaat tcttcccttg ctaaacttgg cgatgtcgtt tttatttcta tttctgaaga
      721 aacagaattc cgatctgaag ctttagcttc ccgtatttct caactaagca ttttggactc
      781 tctatatgtg attttgatgt tttacaacga aaaagaatca cgtcgttcta tcgctaaagt
      841 tcgacatact atttctgaaa tcaaagaata gcaccataac agaaaaagcg aaaaatccat
      901 aaaacaaaaa aagccactac caaatatttg gtagtggctt ttttggttct gcaagggtgg
      961 tacagaacat caatagtcgg tgggttagaa cgtatgtcat cgcatctttg gagaaggttg
     1021 tattggtatt attcgagtta cttgttagat gcgataacaa
//
LOCUS       DAJSKS010000281         1059 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJSKS010000281 DAJSKS010000000
VERSION     DAJSKS010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.292"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(37..993)
                     /locus_tag="ON396_002874"
     CDS             complement(37..993)
                     /locus_tag="ON396_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114903.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgt
//
LOCUS       DAJSKS010000282         1032 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJSKS010000282 DAJSKS010000000
VERSION     DAJSKS010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.293"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..837
                     /locus_tag="ON396_002875"
     CDS             <1..837
                     /locus_tag="ON396_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR3114904.1"
                     /translation="SYRGSFSRKYVLNKWFRLLNIPVIIHLHGSEFKKWYDSVSVKKQ
                     NQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENSIVVPEYTIKWNSKMCRFLF
                     MGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDEEISLKELIEEYNLQSYFEF
                     TGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYGMPVIATDVGDISSAVRDGE
                     NGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAITKFNDGNFFDRILNLYQKGS
                     KE"
BASE COUNT          385 a          109 c          219 g          319 t
ORIGIN      
        1 tcgtacagag ggagttttag taggaaatat gtgctgaata agtggtttag attattaaat
       61 attcctgtaa tcatccatct tcatggatcg gaatttaaaa aatggtatga tagtgtttct
      121 gtaaaaaaac aaaatcagat tcgcttgctg ttaagagagt ctgattccac gattgtactt
      181 ggagaaaaat ggaagaagat aattaaagaa attgagccaa attgtaaaac tgttgttgta
      241 gaaaattcta ttgtagttcc agaatatacc ataaagtgga attcaaaaat gtgccgattt
      301 ctgtttatgg gagttttgat aaagcgaaaa ggtgtacacg atttggtaga agcagttaaa
      361 aacattgcag aacaatatcc agaaaaaaag tttatttttg agattgctgg agcaggtgat
      421 gaagaaatat cgctcaaaga attgatagaa gaatataatt tgcaatcgta ttttgaattt
      481 acaggttggg ttgtgggaga aaaaaaggag gagtgctata gaagatcaaa tgtgatgatt
      541 ttaccgtcct ataacgaggg attgcctata tctattctag aggcgatttc ttatggaatg
      601 cctgtaattg ctacagatgt aggagatatt tcttcagcag tacgtgatgg agaaaatgga
      661 tatttgtttc aacctggtga cgtatcagcg ttgacatctg caatactaaa aatcaatgat
      721 aaagataaat acgtaaaatt ttcacgttgt tcgaaggatt tggcaataac gaaatttaac
      781 gatggaaatt tctttgatag aattttaaat ctgtatcaaa aagggagcaa ggagtgatga
      841 caaggaaaat aaaaaatgtt tcgattatgt aatgaactta ttaagtgaat aaaatagaga
      901 gaaaaaatat tgaaatagac gatgtaaatt tttatccgtg taagaattca aatgaatagt
      961 agtgttttag tgcttgtaat agagcaagag tacaagcacg gtacattata taaaaacatt
     1021 aatgatattt ct
//
LOCUS       DAJSKS010000283         1021 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJSKS010000283 DAJSKS010000000
VERSION     DAJSKS010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.294"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..455)
                     /locus_tag="ON396_002876"
     CDS             complement(<1..455)
                     /locus_tag="ON396_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114905.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKS"
     gene            complement(472..876)
                     /gene="tnpA"
                     /locus_tag="ON396_002877"
     CDS             complement(472..876)
                     /gene="tnpA"
                     /locus_tag="ON396_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HCR3114906.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          308 a          175 c          167 g          371 t
ORIGIN      
        1 gatttgataa tcccttttgg ttgtctatga agcttaattc ttactaatga cttcaattta
       61 ggaactttga tgaatttact atcaatcaaa gcaactgtac cattttgatt attcgttgta
      121 taactctgaa caggattttt cttacttttg aaacgtggaa atccaacgga tttatcccga
      181 aaaaaattct tgtatgcttt atctaaatta agttgggcat tggctaaagc aaggctatca
      241 atttctttca aaaacggaaa ttctttcttg tatttagctg gtgtcggaaa agtcattttt
      301 ttagaagaat catttttaac ttcttcatac gccttttttc ggtcatcaag cattagattg
      361 tagaccttac ggacacaacc aaaagatttg gcaaagaaga tttcttgttc ctctgttgga
      421 tagattctga atttgtatgc ttttagttgt tccataaagt tcacctactt tctatttatg
      481 attgtttcct tgattttgga tatacttctt aatcacatca attggtgcgc caccagttgt
      541 cagaagacaa aaacttttag accagaacat ttctttccaa agaaattgtt tgactcttgg
      601 aaagtcacgt tttatcaatc ttgagctggc acttttatag gcattgatga attttgtcaa
      661 ttctgtttta ggttgagctt tgaacataat atgaacgtgg tctttatcat gattccactc
      721 cactaaagta atgtgatacg attctgaaat tctttcaaaa gtagttttgg cataatcaga
      781 tatttcatca tcaatcactt gtctacgata tttcgttact aaaacaaggt ggtaataaag
      841 aagaaatact gaatgattat ttgtatctaa ttccatgtta tatcaactca tttcttgtat
      901 agactgattg taacatggaa caaaataaaa aggcaatacg ccttagttgt cggtcttcga
      961 gttaccaacg gtaaccctca taccgaacgg cattcatcac ccacctatag aggatgggcg
     1021 a
//
LOCUS       DAJSKS010000284         1003 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJSKS010000284 DAJSKS010000000
VERSION     DAJSKS010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.295"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            370..1003
                     /locus_tag="ON396_002878"
                     /pseudo
     CDS             370..1003
                     /locus_tag="ON396_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          184 g          295 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta cct
//
LOCUS       DAJSKS010000285          985 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJSKS010000285 DAJSKS010000000
VERSION     DAJSKS010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.296"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            268..852
                     /locus_tag="ON396_002879"
     CDS             268..852
                     /locus_tag="ON396_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3114907.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          336 a          135 c          192 g          322 t
ORIGIN      
        1 gattctgaag aaaaaagaaa aaaaagagac caaaaaacat atcaaaaaga aaaataatga
       61 ttttaaaaaa agagtgtcta gtcgacattc tttttttcta attgaaagtg ttcataaaat
      121 cacccatttc tggacacttt ctttttttgc tttatttttc aaaaaaacaa ttcatttcat
      181 gttcataaac ccataacaat atctatgttc agttaaacta tatggataaa gttatgttac
      241 aataaaatta atgaaggagt gatttttttg aaaataggat acgcccgggt atctactggt
      301 ttacaaaatt tggatttaca agaagacagc ctaaataaat ttggttgtga aaaaattttt
      361 actgatcata tgagtggggc aaaaagtaat cgaccaggat tagaaatggc cattgatttt
      421 gttcgctctg gcgatacact tgtcgtttgg cgcttagatc gtttaggtag aaatatggaa
      481 gatttgattt caatcgtcaa tcgactgaat gagcgtggtg tgagttttca tagtattcaa
      541 gaaaatatta cgatggacaa atcaagttct acaggacagc tcatgtttca tttgtttgcg
      601 gcatttgcag aatttgagcg taatttgatc ctggaacgat ccgcagcagg acgagcagct
      661 gctcgtgcaa gaggacggtt tggtggtcgt cctgaaaaat tgtctgaaca agatttggaa
      721 ttactgaaga ctttagtgga tagcggtaca cctattaaga ctattgcgga acggtggaac
      781 gtctcaagaa ccaccattta tcgttatttg gcaaaaatgg aggagaaaaa tggaaagaca
      841 aattctcatt agtttttgtc atttaatgct gaaaattttt gccatttatc tagaaaagtg
      901 acaatagtat gagaattttt tgtaagtgtc tactgataac ttattctcaa atgttgtaag
      961 gattttattc atttgttaat attaa
//
LOCUS       DAJSKS010000286          980 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJSKS010000286 DAJSKS010000000
VERSION     DAJSKS010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.297"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            151..384
                     /locus_tag="ON396_002880"
     CDS             151..384
                     /locus_tag="ON396_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HCR3114908.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            381..788
                     /locus_tag="ON396_002881"
     CDS             381..788
                     /locus_tag="ON396_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCR3114909.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT          376 a          132 c          171 g          301 t
ORIGIN      
        1 aagggagcaa tctaaatttt ttgaattttc ttaattaatt atatagaaag aacaatcttt
       61 ctatagagtt agacatgaga gggactatgc ttgatttaaa atcaacaagc gtatatactg
      121 tatatacaaa gaaaaaggag ggagaagact atggcaatta aagaaagaaa atttagggaa
      181 gtggggaact cagtagtatt aactatttca aaggagtttc ttaaagaaaa tgggttaaat
      241 cccggagata cggttttttt agatgaagaa aaacttcgag cggcaattac aaaaaaacaa
      301 gccttatctg atgaaattga tttagcaata tctcaagcat taactgaata tgatactact
      361 tttagagagc ttgtcgataa atgagtattc attacctaga agccaaagat attataaaaa
      421 tgaatgtaat ccaaataaaa aaatactctc ctaatgaacc ccttggtgta aaggatgcga
      481 atgctttaga aatgtgtgta gggcaacttc gagcctcagc ttttggtgaa gaggtttatc
      541 cttcgattta tgaaaaagca gctatcctac ttatacaatt gataaagaaa catccttttc
      601 acaatgcaaa taaacgtact gctttcttag ctacttttgt ctttttgaaa atcaatggac
      661 agcttttagc tattgatcaa aaagatgctg taaatttagc ggtatatatt gctacgtatg
      721 ataaggattt cgatcaacta aaatatgaag tgatcaacac tataaagaca catacgcagc
      781 ccatttaagg agttactgta tgtgtcttta tagggttatt agctgctata gcgaaataat
      841 tgaagtaaat tacaaatagc tataattcaa atccaactat atagaaaacg taaattttct
      901 atatacttgg atatgctgga atgtttaaga gaataattac atttctagaa aatacaataa
      961 aaattacagg ataattagcc
//
LOCUS       DAJSKS010000287          971 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJSKS010000287 DAJSKS010000000
VERSION     DAJSKS010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.298"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            1..286
                     /locus_tag="ON396_002882"
                     /pseudo
     CDS             1..286
                     /locus_tag="ON396_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
     gene            320..>971
                     /locus_tag="ON396_002883"
     CDS             320..>971
                     /locus_tag="ON396_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HCR3114910.1"
                     /translation="MPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQMYEKIE
                     SNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLSQKKNV
                     IVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTICVLSTT
                     EFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTKLRFL"
BASE COUNT          353 a          112 c          182 g          324 t
ORIGIN      
        1 ggcggatatc agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt
       61 tttgctgtct aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa
      121 tgagatttgg agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat
      181 actttttctt gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat
      241 gcacggtaaa ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata
      301 tgtttatgag gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat
      361 caacaatttt tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg
      421 atcattggaa caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt
      481 aaacaatgtt tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc
      541 aattagtcat ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa
      601 aggattatca caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga
      661 aaaagttaag gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga
      721 ttttaacgca ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat
      781 ttgtgttctt tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa
      841 tctaattaat gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg
      901 tgagttctgc ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct
      961 tcgcttttta g
//
LOCUS       DAJSKS010000288          967 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJSKS010000288 DAJSKS010000000
VERSION     DAJSKS010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.299"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..775)
                     /locus_tag="ON396_002884"
     CDS             complement(<1..775)
                     /locus_tag="ON396_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114911.1"
                     /translation="MMGNLVLSYNIYYFFITFLTMVVSYFSMKQIKNNRYISLLFSII
                     YTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTH
                     LLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQE
                     LKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAFIFIKKLTADDLFIFF
                     LGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFI"
BASE COUNT          403 a          145 c          155 g          264 t
ORIGIN      
        1 ctataaataa agaagaaaaa ccatttaacc gccagacaaa ttgtagactc ttaactggtg
       61 tatggttaaa aagttgccaa ggaaataaat tggttgaaca aaaaagaaca aatacaccaa
      121 gaaagaaaat aaataaatca tcagcagtta atttttttat aaaaataaaa gcaccaatca
      181 atccgagaaa taagaataat ccaatagtat agttgttata gttattattt aaaatgtgtg
      241 tcagcatatc actaggagcc ataccactta attcttttcc cagtgggact ttcaattctt
      301 gtgctctaga ttgttgaata aaaggaatta agaatcccgc acttaaaaaa aaggtgagga
      361 cggtagcttt taaaagactt aagagtctag cgattttttg gtcccaaaaa taaaaagaaa
      421 gaaacaacgt tcctccaata aatacagaaa ccatagcgac agaaagtaaa tgagtataca
      481 ctactagcgt catgccaata gacaaccagt accatttttg gtaatctcta atataaattt
      541 cgtaacaccc cattagtatc aaaggcaaaa atgttaaagc gacagcttct cccaatgaag
      601 cacgatggaa tatgtcaatg gcacgatagg cggaaaatgt ataaataatc gaaaataaca
      661 gcgaaatata acgattgttt tttatttgtt tcatagaaaa ataagaaacg accattgtta
      721 aaaaagtgat aaaaaaatag tagatattat aacttaaaac aagatttccc atcattttat
      781 aaaataaaaa agctggataa agagtaagcc atggataaaa aatattcatc attgttccat
      841 gatgatcgaa gttattgaag tttactggag aactccatac attatctagt cctatgattc
      901 gagataaatg aaagtaagta tcctgattga cactatgata gattcccata gacatgaata
      961 gagggat
//
LOCUS       DAJSKS010000289          943 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJSKS010000289 DAJSKS010000000
VERSION     DAJSKS010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.300"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..>943
                     /locus_tag="ON396_002885"
     rRNA            <1..>943
                     /locus_tag="ON396_002885"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAJSKS010000290          937 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJSKS010000290 DAJSKS010000000
VERSION     DAJSKS010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.301"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            63..383
                     /locus_tag="ON396_002886"
     CDS             63..383
                     /locus_tag="ON396_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3114912.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            401..832
                     /locus_tag="ON396_002887"
     CDS             401..832
                     /locus_tag="ON396_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCR3114913.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT          338 a          136 c          159 g          304 t
ORIGIN      
        1 aaattatttc tgcgtatttt tctaaacttt ttttgtaaac atttagaaag gttgttttat
       61 cgatgaatac ctatgaaata ataaaagact tggcgaagag gaaaaagata tctatacggc
      121 agttagaaat taattttgga tattctaacg gttatctagc aaagtggaaa aataatactc
      181 ctaattcaga tgaattaagt cggattgctg actatttcga tgtctctgta gactaccttc
      241 taggtagaga agaaagagaa acccctaaac atgtggattt atcagaagac gatactgttt
      301 tttcttttga tggaaaagaa atatctaagg agacaatgcg taaagcgatt gcaattgcta
      361 aagctttaga ggaaaatgaa tagttggagt gatggtttgt atgtatttga aattgaaaga
      421 aatgttgagc gaatacaatt tgaaagtagt ttatatggag atgaaagagc ctggtttcta
      481 ctatccaaaa cccagaatca tttttttgaa tgaaaatcta tacggtgaaa ctgcagaagc
      541 ctttcattta tctcatgaac ttgcacattt cagcgcctct cattttgaat tttcagtttt
      601 gtacgatact tctacaactt ttcactcaaa atttgaaaca gaagcagata aaatagctat
      661 tctaatttta ttaaatatct atattgagaa cgaactgact gatgaatctc agtttaatct
      721 cgaaaaattt atggagtatt attctattca gaataagctc agatacactt gttatgctgt
      781 ctgccaatgc tattttaaga aaaaatattc ttacgcaagg caatatgtat gaatacatca
      841 agaattatta aaaaaaccga tttcagcaaa acattaaaaa agcccgtgct gcaacacggg
      901 ctctttcctc atatatgagc ttctacaaaa aaaatca
//
LOCUS       DAJSKS010000291          910 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJSKS010000291 DAJSKS010000000
VERSION     DAJSKS010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.302"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..258)
                     /locus_tag="ON396_002888"
     CDS             complement(<1..258)
                     /locus_tag="ON396_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114914.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFM"
     gene            490..>910
                     /locus_tag="ON396_002889"
     CDS             490..>910
                     /locus_tag="ON396_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3114915.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQE"
BASE COUNT          328 a          154 c          139 g          289 t
ORIGIN      
        1 catgaaacta tcaatatcag attttttaaa tctaatgacc gtatcaattt ttataacagg
       61 taacccgtat tgagaaacat attttctaat cgttcctact gatacaccta gatattcact
      121 tgcctgctga tagtccatat actctagagt gactccagaa ataatgtctt ctagcatagg
      181 ctttagcact gtaattatct ctttcacttg ttgttcaact tgtatagaaa caatgctatc
      241 aatgatttgt agagacatat ttccccctcc tttcgtgatt gcttgaacaa attttctaag
      301 aattatcttc gtattttttg ctcaaaataa aagtattttg ttattaaccg attaatcaaa
      361 caaaaaatga ttaccagttc atcgacatac acataatacc tcgataaaaa agaggtgtca
      421 atatctttgt aaaatttatt tacaacaccc ctttttcgag gtatactaat tttaggaggt
      481 gtggtcaata tgataaaacg tccaccaata aattatttag aacgaaaaaa gatactcgga
      541 acaaaaataa aagctataag aaagagtaaa aaattaacac aaccagcatt tggtcttatg
      601 attaacaacg gtcaattaat tgacaaaaaa actatctacg aatgggagaa gggaacctat
      661 ttaccaatac cagagcgatt atcccgaatt gctgatttgg gaaatatgag tatagaggag
      721 ttagtttgtg gcaatgtaga agaatatatt ttgggaatta ttctttatag agatagcatt
      781 gtactagatg gtataacatt tccagataaa aacctgttcc agcatcttcg tcaacagttc
      841 ccaccagtac actctaattt agatacatgg ctggatagat attccaaatt agaaccagag
      901 atgcaagaat
//
LOCUS       DAJSKS010000292          899 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJSKS010000292 DAJSKS010000000
VERSION     DAJSKS010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.303"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            256..879
                     /locus_tag="ON396_002890"
     CDS             256..879
                     /locus_tag="ON396_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3114916.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          333 a          129 c          167 g          270 t
ORIGIN      
        1 aataaactaa tgtccttatt tttgtgttca catttctgtt acctaagaaa tacttaaagg
       61 aggtgatgta gccagcaacg actacaactt gaacccgcta tctacagttt atttgttgtt
      121 tcttttgggt atactctaat ggtacaaatc catatttgct taaagcgttg atttttcagc
      181 ggtttttctt tattgcaaag tgtaccctaa gacctgtata ctaatagtaa atagattgga
      241 taggaatgat ggcaaatgta tttagcttat ttaagagttt cttccgacga tcaaagttta
      301 gctcggcaac ataagctaat tgaaaattgg tcagtcaaaa ataatattgc tgaagacgat
      361 ttaaaaattt tcgaggaaaa agtctctgga aaaaatattg aaaacagacc agagctgcaa
      421 gagcttattt ctttcatacg tgaacgagat acgattatta ttcctagtct tgatcgatta
      481 gcaagaaata gtaaagatac aaaagactta cttcaattgt ttcgtagtaa aggtgcttct
      541 attgaaatat tagacctacc gtcgtttaat ggggtaactg accctgcatt aagagacttg
      601 ctaacaaatt tagtcataga agtgtttagc tatgttgctg aaaacgaacg gaaaaaaatc
      661 aaagagcgtc aaaaacaagg cattgaaatt gcaaagaaaa atggtcgtta tactggtaaa
      721 aagattcaat accatgccaa tgcaacagga aaaaacaaat tgatttacga tgaaattgtt
      781 gatggtctga agaaaaaaga aactatttct aaaatagcaa agaacgcagg agttacaaga
      841 aaaaccgttt atcgagtaaa aagagattta ggaatgtaat atgttccagt tagaaagaa
//
LOCUS       DAJSKS010000293          887 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJSKS010000293 DAJSKS010000000
VERSION     DAJSKS010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.304"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            4..>887
                     /gene="bsh"
                     /locus_tag="ON396_002891"
     CDS             4..>887
                     /gene="bsh"
                     /locus_tag="ON396_002891"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCR3114917.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQIT"
BASE COUNT          300 a          121 c          162 g          304 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattac
//
LOCUS       DAJSKS010000294          875 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJSKS010000294 DAJSKS010000000
VERSION     DAJSKS010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.305"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..792
                     /locus_tag="ON396_002892"
     CDS             <1..792
                     /locus_tag="ON396_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR3114918.1"
                     /translation="FCGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKK
                     GQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSF
                     KGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEI
                     IRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEY
                     SIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
BASE COUNT          325 a          118 c          147 g          285 t
ORIGIN      
        1 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
       61 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      121 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      181 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      241 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      301 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      361 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      421 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      481 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      541 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      601 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      661 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      721 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      781 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      841 tttatgtgag cgttttttat aatgcaataa agaaa
//
LOCUS       DAJSKS010000295          867 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJSKS010000295 DAJSKS010000000
VERSION     DAJSKS010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.306"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            15..>867
                     /locus_tag="ON396_002893"
     CDS             15..>867
                     /locus_tag="ON396_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR3114919.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEE"
BASE COUNT          301 a          137 c          172 g          257 t
ORIGIN      
        1 aggaggactt tctcatgact aaatttacta tagaaattat gcaaacacta ctcaataaag
       61 gggatttaga tgaattattt caggatcatt tagagcgagc gattaattca cttctacaag
      121 cagagctaac tgctttttta gattacgaaa aatatgatcg aaatggattc aattcaggaa
      181 attcccgtaa cgggaattat tcacgtacat ttaaaacaga atatggcgag ttgaatttaa
      241 ctattcctag agatcggaat ggcgaatttt ctcaacaaac actaccagca tataagcgaa
      301 ccaatgattc attagaaacg accattatcc aattatttca aaaaggcatt actatggctg
      361 aaatctccaa attaattgaa aaaatgtatg gtcaccacta tacgccacaa acaatctcca
      421 acatgagtaa attggtggct gaagatgttt tagcttttaa agaaagaacg ttagaagcca
      481 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
      541 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      601 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      661 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      721 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      781 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      841 tttaccaagc gaattcaaaa gaagagg
//
LOCUS       DAJSKS010000296          850 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJSKS010000296 DAJSKS010000000
VERSION     DAJSKS010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.307"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            101..814
                     /locus_tag="ON396_002894"
     CDS             101..814
                     /locus_tag="ON396_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114920.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT          287 a          160 c          149 g          254 t
ORIGIN      
        1 aagaagatac actctattta tattcataga caaggtttta cggattcaaa aattcaggat
       61 tatgctatac tggtattaaa aaaataatga ggtattttat atgaaaaagt taggttttat
      121 tttattgttg gtctctttat tccttgtagg atgtacgtct cctagtaaag aaaatagtga
      181 tccatctgcg accacagaca aaagttcaat cagacaaacc agttcagctg aaaaatcaag
      241 tactcagaaa tcagcttcta ctgaaacgaa tacatcttca acaagcagta aagtctcaga
      301 gcccaatact tcttctacat ctagtagtac agttgagaca aatatttctt cttcaacaat
      361 agacacaacc tcctcacagt cttctcagtc aatagatccg ttagcaggat attctgatct
      421 acaaattgag tacgcacgtg tttggctagc tattagaggt aatactttta gagatacatc
      481 gggtgatttc gaattacatg ttattcatta tcctgcagga acacctatcg ccccttatga
      541 tgcagggagt gcagtctatc cgaaagatgt ggttatgctc actgggaaat acagttacca
      601 gggagtttta gtgtattcaa gtaaccatga cgggacaatc actagttatc cagttccttc
      661 acattggcaa atgcctgcag atcaaacatc ttatccagcg ttcattagag aaacaactca
      721 agaaatcatt gataatgcat ctgtagtagc tattcctaca ggaaatccca atgacgtgaa
      781 acagttaatc gatgtgacag ttatagataa ttagactttc taatatgtta tacaaaaaga
      841 agctgacagc
//
LOCUS       DAJSKS010000297          850 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJSKS010000297 DAJSKS010000000
VERSION     DAJSKS010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.308"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(37..109)
                     /locus_tag="ON396_002895"
     tRNA            complement(37..109)
                     /locus_tag="ON396_002895"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(74..76),aa:His,seq:gtg)
     gene            complement(112..185)
                     /locus_tag="ON396_002896"
     tRNA            complement(112..185)
                     /locus_tag="ON396_002896"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(151..153),aa:Trp,seq:cca)
     gene            complement(192..272)
                     /locus_tag="ON396_002897"
     tRNA            complement(192..272)
                     /locus_tag="ON396_002897"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(236..238),aa:Tyr,seq:gta)
     gene            complement(274..349)
                     /locus_tag="ON396_002898"
     tRNA            complement(274..349)
                     /locus_tag="ON396_002898"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(314..316),aa:Phe,seq:gaa)
     gene            complement(385..457)
                     /locus_tag="ON396_002899"
     tRNA            complement(385..457)
                     /locus_tag="ON396_002899"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(422..424),aa:Asp,seq:gtc)
     gene            complement(467..539)
                     /locus_tag="ON396_002900"
     tRNA            complement(467..539)
                     /locus_tag="ON396_002900"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(504..506),aa:Val,seq:tac)
     gene            complement(555..626)
                     /locus_tag="ON396_002901"
     tRNA            complement(555..626)
                     /locus_tag="ON396_002901"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(591..593),aa:Glu,seq:ttc)
     gene            complement(641..730)
                     /locus_tag="ON396_002902"
     tRNA            complement(641..730)
                     /locus_tag="ON396_002902"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(694..696),aa:Ser,seq:gga)
     gene            complement(770..843)
                     /locus_tag="ON396_002903"
     tRNA            complement(770..843)
                     /locus_tag="ON396_002903"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(807..809),aa:Asn,seq:gtt)
BASE COUNT          237 a          234 c          193 g          186 t
ORIGIN      
        1 tgccttaccg cttggctata ccccaagaat aaaaagggcg aatgatggga atcgaaccca
       61 cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc atggcagggg
      121 cagcaggaat tgaacccaca ctaacggttt tggagaccgc tgttctacct ttaaactatg
      181 cccctataaa atggaggaga gtggattcga accactgaac cctaaggaac ggatttacag
      241 tccgtcgcgt ttagccactt cgctactcct ccatggtggc gcgggacaga atcgaactgc
      301 cgacacacgg agcttcaatc cgttgctcta ccaactgagc taccgcgcca tattttaaaa
      361 ttattaattg aataacaaga acgacggttc cgacgggaat cgaacccgcg atctcctgcg
      421 tgacaggcag gcatgttaac ccctacacca cggaaccagt tcattatgga ggttaacggg
      481 atcgaaccgc tgaccctctg cttgtaaggc agatgctctc ccagctgagc taaacctcca
      541 acattcaatt aaaatgaccc gtacgggatt cgaacccgtg ttaccgccgt gaaagggcgg
      601 tgtcttaacc gcttgaccaa cgggccaggt aaatttgcta cggagagtaa gggattcgaa
      661 cccttgagac agtttggcac catctacatg atttccaatc atgctccttc ggcctctcgg
      721 acaactctcc agagtgaaag aagctcaata ctctttctca aaatgagaac tccggcagta
      781 ggactcgaac ctacgacatc atgattaaca gtcatgcgct actaccaact gagctatgcc
      841 ggaataacat
//
LOCUS       DAJSKS010000298          847 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJSKS010000298 DAJSKS010000000
VERSION     DAJSKS010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.309"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(60..305)
                     /locus_tag="ON396_002904"
     CDS             complement(60..305)
                     /locus_tag="ON396_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114921.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(299..700)
                     /locus_tag="ON396_002905"
     CDS             complement(299..700)
                     /locus_tag="ON396_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3114922.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT          280 a          146 c          114 g          307 t
ORIGIN      
        1 attccttaac tattagaaga ttcttttaaa cagaaaaagt ggaaagggga tttttttaat
       61 tagttaattt taaattttaa gatcgcttct attaatttaa tttctagctg acttatcata
      121 tattcatcta cataggtaac gaattgacct acctcgttaa atccatttgt caaacataac
      181 ttagccatat aagaatgata atgtttgagt atcatcttga tcgaaattgg atctccttct
      241 agagcagata agataatgga tagtggaact aataggtact gccgcttttt agatttatct
      301 accataggtc ataccccatt ttcaattgtt caactgcctt ctttcgttga taccagatac
      361 cacttgtgct catattgaga attttaccga tttcagcatc attaaaacct gcaaaataat
      421 aaagtaggat aatctttctt cttatctccg ataactgatc aattgctgct gataactttt
      481 gatctgaaat taatatttcc attccaaata agaaatagac atcttgttca tcaaaatgag
      541 tttgggaata cctgccttca tttgaatctg ccaatggttc ctcattatcc tttatgttag
      601 cgtttcgttt tcgaatagac ttagcttcgt ttcgtaatac ttttttacag taggcatcaa
      661 attggtgttg tatagatatg tcattgtaat gttgttgcat gataatatgc tcctttttcc
      721 ccctttccac taatacaaca atcagaaagc cccttttttt cgaaaaaact agaaaaaaaa
      781 cataaaaata tttatcattc tttaaacaga aaatctttct ataaaaaaca aaaaaaaacg
      841 attaggc
//
LOCUS       DAJSKS010000299          828 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJSKS010000299 DAJSKS010000000
VERSION     DAJSKS010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.310"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            30..>828
                     /locus_tag="ON396_002906"
     CDS             30..>828
                     /locus_tag="ON396_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR3114923.1"
                     /translation="MNQSVAKTAHCLNRSRQTIHKVYLFFKQGKSALEYYQQYKKNKS
                     NCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSD
                     LPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVIT
                     LVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNV
                     NDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSV"
BASE COUNT          281 a          141 c          158 g          248 t
ORIGIN      
        1 aacttgtaat aatagagtct tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt
       61 gcttgaatcg ttcaagacaa acgatccata aagtatatct gttcttcaag caaggaaaat
      121 cagccttaga atattatcaa cagtataaga aaaacaaatc aaactgtggt agacgtccgc
      181 ttgttttacc cgaggaacaa tcagaatata ttcaaagaaa ggttgttcaa ggatggacac
      241 ccgatgtgat tgttggtcgt gcagcgtttc ctattagttg ttctgctcgt accatttatc
      301 gtatgttcaa aaaggggcta tttgattctt ctgacttacc gatgaaaggc aagcgtaaac
      361 cgaatggaca tcaagaaaga cgtggaaaac aaactttccg ccgctctatt catgaacgtg
      421 aaaaggatta tagccaattc tcaaatgagt ttggtcacct tgaaggtgac actatcgtag
      481 gtctgaaaca taaaagtgct gtaattacct tagttgaacg attatcaaaa gttatcatca
      541 cattgaaacc gtgtggtaga caagcgattg atattgaaaa aaaattaaat caatggtttg
      601 aatctgtacc gaaaaaccta ttcaaatcca tcacttttga ttgtggaaag gaattttcaa
      661 attggaaaca gatcagtaat gtcaatgata ttgccattta tttcgctgat ccaggaacgc
      721 cgtctcaaag aggcctaaac gagaattcta acggattgtt acgtagagat ggtttattga
      781 aatctatgga tttcaattca gtagatgaat tttttattca atctgtcg
//
LOCUS       DAJSKS010000300          823 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJSKS010000300 DAJSKS010000000
VERSION     DAJSKS010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.311"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            22..>823
                     /locus_tag="ON396_002907"
     CDS             22..>823
                     /locus_tag="ON396_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR3114924.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT          253 a          142 c          186 g          242 t
ORIGIN      
        1 cgaggagggt ggaaagaaac cttgccagaa attgcggatg aaatcagtat cctcaacttg
       61 acgagtaagt cagaaaatca aggtctgctt gatccttatg tgattatgaa gaaaaagaaa
      121 gactcggaaa gtttagcaat tgatattctg acttttttaa caggaatttc aagtcgagac
      181 ggggagaagt ttcctgtgtt acgtaaagcc attcgaacgg tgactcaaag tgatcaacgg
      241 gggttattac aagttattaa cgaattgcga aaagacgggt caccaatcgc ggaaaatatc
      301 gccgatcata tcgagtcctt taccgattat gattttgctc atttgctttt ctcagacggc
      361 tcgattaccc aatcgattag tttagataaa cagctaaata tcattcaagt ggcggatttg
      421 gttttaccgg atgcagaaac gacttttgaa gaatatacca ccatggaatt gcttagtgtt
      481 tcgatgctaa tcgtcatttc cacttttgcc ttggacttta tccatagtaa ccgagaaatc
      541 ttcaaaattg tcgatttgga tgaggcttgg agttttcttc aagtggctca aggaaaaacg
      601 ttatcgaata agcttgttcg tgctggtcgg gccatgaatg ctggggtcta tttcgtcact
      661 caaaatagcg acgatttatt agatgaacgt ttaaaaaata atatcgggtt gaagtttgct
      721 tttcgcagta ccgatattca tgaaatcaag aaaaccttgg aattctttgg tttggatcag
      781 gaggatcaaa gcaatcaaaa acgcctgcga gatttggaaa acg
//
LOCUS       DAJSKS010000301          804 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJSKS010000301 DAJSKS010000000
VERSION     DAJSKS010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.312"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..>804
                     /locus_tag="ON396_002908"
     CDS             <1..>804
                     /locus_tag="ON396_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114925.1"
                     /translation="KNTQSIYTTGDLSFHLSRIKGLSTIFTSPINYETFNYTGYGVNY
                     FYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNLCTTLVAYHYGERFLKQKKAAFLFSC
                     LYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFYAYQIISGKEEKYPSVKLALSMSLLV
                     YSHVLSTLMSTALIIIFIFIRLVSKGFKNADFIAIFKKLFSAAGMTLVLTSAFWYPMF
                     EQMLYQKINKPSVTNLYAHASNVFDSLTEAMNNDLTTYSMGLVGLLSLC"
BASE COUNT          240 a          131 c          134 g          299 t
ORIGIN      
        1 tcaaaaatac acagagtatt tataccactg gagatctttc ttttcactta tctcgaatca
       61 aggggttatc gactattttt accagtccga ttaattatga gacattcaat tatactggat
      121 atggtgtaaa ttatttttat ccctttctga ctttttttcc agctgtaatg ttatattgga
      181 tgacaaaaaa tttgattgta agctatatta tttacgtttg gttattgaac ttgtgtacaa
      241 cactcgtagc ttatcattat ggcgagcggt ttttaaaaca aaaaaaagcg gcatttttat
      301 ttagttgtct ctatattttc tctgcttatc ggacagtaga catctactat cgttccgcaa
      361 ttgccgaagc tatcgcaata acacttgtta tccctgtatt attttatgct tatcaaatca
      421 tctctggaaa agaagaaaag tatccgtctg taaagctagc attgagtatg agtttgctag
      481 tgtacagcca tgtactatca acattaatga gtacagcatt aattatcatt tttattttta
      541 ttcgtttggt ttctaaaggt ttcaagaatg cagattttat tgctatattt aaaaaattat
      601 ttagtgcggc aggaatgaca ttggttctga cgagtgcttt ttggtatccg atgtttgaac
      661 aaatgctcta ccaaaaaatc aacaaaccta gcgtgacaaa tttatatgct catgcatcta
      721 atgtgttcga tagtctgact gaagctatga acaacgactt aactacttac tcaatgggac
      781 tagttggtct gttatcttta tgta
//
LOCUS       DAJSKS010000302          802 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJSKS010000302 DAJSKS010000000
VERSION     DAJSKS010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.313"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            121..735
                     /locus_tag="ON396_002909"
                     /pseudo
     CDS             121..735
                     /locus_tag="ON396_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          294 a          131 c          145 g          232 t
ORIGIN      
        1 aaaaccgttt gtctatttag agtttaccct aaatagacaa aaatataaca ttgaaaaatt
       61 tagagttcta acgtatgatt agggtgtatt ataaacgtta tagaaaagta gggaataaat
      121 ttgaaaattg cttatattag agtttcatcc atcgatcaaa atgaacaacg tcaaattgaa
      181 gaaatgaaaa agtttggcgc tgaaaaaatt tttatcgaaa aacaatctgg agcgacgatt
      241 agtcatcggc cgattttcca ggaagcttta gattttgtca gagagcaaga tatttttatt
      301 gtagaagcca ttgatcgact tggacgcaat tatgacgaga ttatcgattc agtgaattat
      361 ctaaagaaga agaatgtgcg acttattatc accagtttac cgatcatggc tgaagcaatt
      421 ggaaatcctc tcctagataa attcatcaaa gacttaatca tttaaatctc agccatgatt
      481 gctgaacaag aacgtactga atcgaaacga cgccaggcac aaggaattaa aatcgctaaa
      541 gcaaacggcg tctataaagg tcgtcctaag ttatacagtg cagacaccaa agatcctcaa
      601 cgtcgccttg tctataggag tatcgttcag gatcttgaga atggggttgc tatctcaaag
      661 attgcaacag actacaatgt gactaggcaa acaatttacc gaataaaaaa agaaatcgac
      721 caactaattg tatgatttct tttttgaatc acgtaattat taattagctt tgtcatcaat
      781 acagacaatc attttagatt ca
//
LOCUS       DAJSKS010000303          801 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJSKS010000303 DAJSKS010000000
VERSION     DAJSKS010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.314"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            63..653
                     /locus_tag="ON396_002910"
     CDS             63..653
                     /locus_tag="ON396_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HCR3114926.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
BASE COUNT          293 a           88 c          169 g          251 t
ORIGIN      
        1 ctcttgttgc aagaaatccg aataaaattg ttaagtataa attgaattaa taggagatat
       61 gaatggaaat tagcagtaaa gaatatttaa aagcggttct tcgtatggaa aagaatctat
      121 atatagaatc taaaggacaa cacacaaaat tactaaaaat aatgaaatca catccggact
      181 acaacgcttg gcggtatact cgtcgaatga gaattacatc ttattattat tcaaagagaa
      241 agaaaaatat catttatgcg attatgtata ttgtgtcttg cagaagaatg aatagtttgg
      301 gaagaaaatg tggtatagag tgtggtgaaa atgtatttga tgaaggtgta agattttacc
      361 atacacaagg aattgttatt aatggtaacg ccataatagg aaaaaattgt tacttatatg
      421 ggaataactg tattggaaat gatggaatcg atcctaagtg tcctgtaatt ggaaataatg
      481 tacgtgtatg cgtgggtgca aagattattg gtaatgtaaa aatagctaac aatgtagttg
      541 ttgcggcagg agctattgtt atcaaagatt gtctagagaa tggtgcaatt cttgcaggtg
      601 taccggcaaa aataataggg tatgttaatt taacaaaagt atttttagaa taatttttag
      661 cgaaagaagg tgaataacga tgcgtatttc aataaaaaga tggtcaggag cagattggac
      721 attgttcacg ttggcatttt ttgcaatctc catagttctt gtatatactt ttaacgcccc
      781 aagagcaatt ttatatttgg g
//
LOCUS       DAJSKS010000304          793 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJSKS010000304 DAJSKS010000000
VERSION     DAJSKS010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.315"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            2..718
                     /locus_tag="ON396_002911"
     CDS             2..718
                     /locus_tag="ON396_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HCR3114927.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
BASE COUNT          283 a          151 c          159 g          200 t
ORIGIN      
        1 attgaaaaaa aggattttag taacaattgg tctagctttt ttgttagctg gttgtaccaa
       61 cgggaatgaa tcgaaaaaag aatcaacaaa agaaacagcg aaagcaacag ttgcaactac
      121 tacaacaact agcacagaga gcaagtcaac tgcagcaagt acagaaacag cagatacctc
      181 tacttcgtca gagtcttcca aaacgactga aacaactcaa acatctactt caagttcttc
      241 tgtagaggct tcatcttcct cttcagaaag cagcgaacaa gttgaacaaa cactgtggaa
      301 tgcagacaaa tccgcacaat tagaggcatt tatgaccaaa tggggccaaa caatgggaca
      361 acaatacaaa agttatacga atcaaatgag tgtggactta tatggtctta aagtccctca
      421 agctattttg aacggggagt ggaagatggc tattggtgga gtgcctgttt ctgcagaatg
      481 gtcagaaagt gggacaggtc aagcagacta tcaaatcaca gccgtctatt ccgatgcaga
      541 gacagaacca tatctgaaaa agcacgtcta tctattcggg tttcagcaaa atcagccaaa
      601 agtattggtg acacaacaaa atcaggggaa tcccgataat tatctttact tcaacgagac
      661 agcgaataat gaattgaaaa atggctttaa tcaaatcgtt tatggacaag caaattaatg
      721 agataaacaa ggggttgtga catgtctttt atcacaaccc cttgctcagt ttatttgtta
      781 gtcttttatt ttt
//
LOCUS       DAJSKS010000305          785 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJSKS010000305 DAJSKS010000000
VERSION     DAJSKS010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.316"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            121..>785
                     /locus_tag="ON396_002912"
     CDS             121..>785
                     /locus_tag="ON396_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR3114928.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFK"
BASE COUNT          308 a          116 c          121 g          240 t
ORIGIN      
        1 ttttcttttt ttcatgtatt cttttatttt tataactagt gtattacttc aaaatttgat
       61 attatatggc atgaacagaa ttaaaatatc agttaaaata aaagttaagg agcattgtgt
      121 atgacaatta aaactagtct ccaaaatcaa gcttaccaat ctattcgccg acaaattatc
      181 tatgctgact tagaacctgg aaaaaaagta tcagaaaaaa aattagaaga aacactttct
      241 attggtcgta caccaatacg tgaagcactg attcaactcc gacaacaagg tcttgttgat
      301 acaattcctc aaagcggaaa ttatatctca ttaattgatt tagattcggc caaaaatgct
      361 cgatttgtca gagaacattt ggaacgacaa attatgttag agtgttgtgc caaaatgaat
      421 aaccaaaaaa aacggatttt agaaactata atttcagaac aagaaaaagc tgttagtcaa
      481 aaaaatgctc gtagtttttt tcataatgat aatttattcc ataaagcttg ttttgaaatt
      541 gcagagcgag aagaagtatg gcaatggtta gaaaataaca atacacactt agaacgattt
      601 cgatggctac gagtaaaaac ggaaggatta aagtgggaaa aaattatgga acaacattat
      661 caactatttg aggcattaac aaaacaagat ccagaagaag ctaactttct aacatcgttg
      721 catcttcatt taatgcttaa tgagcaaaat attgttttag ctactcatcc aaaatatttc
      781 aaaaa
//
LOCUS       DAJSKS010000306          780 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJSKS010000306 DAJSKS010000000
VERSION     DAJSKS010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.317"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(4..258)
                     /locus_tag="ON396_002913"
     CDS             complement(4..258)
                     /locus_tag="ON396_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114929.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(422..745)
                     /locus_tag="ON396_002914"
     CDS             complement(422..745)
                     /locus_tag="ON396_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114930.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          328 a          131 c          117 g          204 t
ORIGIN      
        1 tactcaatgt tttttattta aacgtatcaa ataaagaatg acgatcaata aagacaaaac
       61 cagtcctgat aattcttcta taaaaacact atcgggttca aagtagttgt aattaaaaac
      121 tttccacata aagtagatag ccggaagagt caaaatagct aaataaaacc gttctttgtt
      181 ccaattcata agataaacaa tacttgcgac cagtgcgacc gcaggcaccc aaaacaaaac
      241 tctcaccaac gtaatcatgt aacggactcc tcaacgattc atataataaa agataacatg
      301 ttattgcata catcacaaat gaaatgctag gaaaataaaa gaagaataga tcaatgaaaa
      361 atgaaaaagc agaacagttt tctgctgata agcaggaact gttccgctga atgtgtctta
      421 tctatcgaga aaaaatcaat ggattgatat ctaagtaatt caatacatat aaaaagagga
      481 cgatcgcatt cactaaaaag gaaagaatga tgatcccata gcgaatggaa acatatttaa
      541 caaatacttt tgtttgaata cgggcatatc tttttccttt atagctgagc atcatgacaa
      601 gaagcaaaag ggtaatcaca ccttgaaaaa tcattccaat aaacagaatt cctgaggttt
      661 gataatttaa ttggagaaaa taagctgctg ccgcaactaa atcgaataaa atcgcaataa
      721 atggcaaaat aatcactcct aacattgaga ataaataaaa gaataacatt ttttctaaaa
//
LOCUS       DAJSKS010000307          780 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJSKS010000307 DAJSKS010000000
VERSION     DAJSKS010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.318"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(27..>780)
                     /locus_tag="ON396_002915"
     CDS             complement(27..>780)
                     /locus_tag="ON396_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR3114931.1"
                     /translation="SRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILL
                     VLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEIS
                     AGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHN
                     GFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMV
                     EAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          294 a          143 c           93 g          250 t
ORIGIN      
        1 tccaagcaat gaaagctgtc ttcatattaa ttctcctttt ttaaatacaa gtaagaaatg
       61 cttcccaaat aataccagaa aacaattgaa atgacatcgc gcttattgaa aagtacatga
      121 gcctccacca tttcatctat aaaaatagat agtataaaaa ttaaaacaat accaagaaac
      181 gcataattca ctttacttct aaaaaatgtt ttgagtaaac taaagatatt taacacatag
      241 aaagcataga tacatataaa agcaattata ccacttgata caagtacttg aagaaatcca
      301 ttatgcatgt ttccatgtgc tctttttagt tcattcacat ctgtagtatc aagatctact
      361 tttttcaaaa attttgcatc gctattccca tagaaatcgg tctctccata accaaataac
      421 aaagttggtc ttataacttt aagcgcagct ttccaaatcg taaaacgacc agctgaaatt
      481 tcggagttat cactagaatg ctgtaatatt acttcttttt ctggaagtgg agttttatct
      541 gattcatttg actctataac ttctactttt ctatcaatcg atacaggaat tgttgctaaa
      601 aaatccttag cataattagt accaaaagaa aaatatacca atagaactaa taatatgctt
      661 accatccatt tgatttttac aggtagtact tctctaatgc tctttctaaa ttttgaattg
      721 gcaaacaaca aaagaaataa tactaaaaaa gaacaaatta ctacctgggc acctctagac
//
LOCUS       DAJSKS010000308          774 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJSKS010000308 DAJSKS010000000
VERSION     DAJSKS010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.319"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
BASE COUNT          270 a           95 c          165 g          244 t
ORIGIN      
        1 aaaaaattta taaaaggagg tttcttgata tgataagaaa caaaaagggt ttgttgaaag
       61 ttgctttact ggcagctttt ttctaagtaa ctttttgaca agctcaatgg caacacaagc
      121 ggaagaagta atacaagaac taactgttat gaataacagc tagataaaat gggctatgta
      181 tgttatccaa caatttagta atcactagaa gcctagcgaa agttcgcttg ataagttata
      241 gatgagtatt gatttgtagt gtttccaaat caaaagggta cagagccaca tgaaatatgt
      301 tgtcctctgc agcgtcagga tgggaatcca taccatggag attcggagat ctgtgaggga
      361 atacttacgt ggttaatatg tcagttgtgt tggacgtagt aatggtcata gtgggtagat
      421 tgactgaatt cttatatgag aaaatgaaac caacaagaag atagggttat ttatacagaa
      481 ttggggatct tttctggaaa agtaatcgga taatgcgaga ttcgtacggt cttttttaaa
      541 tttctctaaa aaaaacggca tcgaatgggt tgggtgattg ataagcaatg aatcatagac
      601 tgatatagaa taaaaaatga ttctatttat ctttttacat ctatcgtttt ttcaagtagc
      661 cgcaaattat atcagcattc aacaattttt gtatacaagt tctagtaaaa ggatctgaag
      721 aaaaagttgg ttgttgtgaa gataaaaaaa gaatcaggtt ttactgagtt tttc
//
LOCUS       DAJSKS010000309          760 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJSKS010000309 DAJSKS010000000
VERSION     DAJSKS010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.320"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            68..253
                     /locus_tag="ON396_002916"
     CDS             68..253
                     /locus_tag="ON396_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3114932.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            266..514
                     /locus_tag="ON396_002917"
     CDS             266..514
                     /locus_tag="ON396_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114933.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            545..715
                     /locus_tag="ON396_002918"
     CDS             545..715
                     /locus_tag="ON396_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114934.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
BASE COUNT          275 a          103 c          165 g          217 t
ORIGIN      
        1 aataggtgtc aacgatttag ctaatttttt agcttacaaa ttatttgttt agaaaggagc
       61 tatttttatg tctgagaatt tagacttaaa aattcgagcg gagatgagaa aaagaagaat
      121 gactttcaaa gaactagctg cgcttatcgg tatttcagga gcttatttat cagatattct
      181 aaacggcaat cgtgatggaa agaaagcaca acagcatatc gaaacagtga aaaaaatatt
      241 ggacatccga tagggggggt aggcgatgac aaaactaaaa aaacaagatt ttgtaaaaaa
      301 atataattat tctccatcta cttatcaacg tcgaatgtcg gaactaaaaa atacagcaat
      361 tttctcagcg gcgtatgaac gggtcacagg acaagaagtt tggatcaata cagaattata
      421 cgataaattt ttgtctttca aatcctataa caggttacgc acaagaaagg taacacctaa
      481 agaatttatc gagaagcatt tagttgattt ataaaaaaca gaacattttg agaggtgaag
      541 gttaatgggt aaattcaaca gagcattagt attcagcgca ccgctaatcg tctatgcttt
      601 agggctttgg ggaagtagac aagcgttgat aggaacgatt gtgtacatgg tttggatttt
      661 tatagggctg gatgaagctg agtacaaaac aaaaaagcca gtcgggaggg actgactaat
      721 gagtaaggac gatattgtat ttctgttaat tatgttttgt
//
LOCUS       DAJSKS010000310          745 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJSKS010000310 DAJSKS010000000
VERSION     DAJSKS010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.321"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..>745)
                     /locus_tag="ON396_002919"
     rRNA            complement(<1..>745)
                     /locus_tag="ON396_002919"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          152 a          231 c          166 g          196 t
ORIGIN      
        1 aaggggaagc tctatctcta gagtggtcaa aggatgtcaa gacctggtaa ggttcttcgc
       61 gttgcttcga attaaaccac atgctccacc gcttgtgcgg gcccccgtca attcctttga
      121 gtttcaacct tgcggtcgta ctccccaggc ggagtgctta atgcgttagc tgcagcactg
      181 aagggcggaa accctccaac acttagcact catcgtttac ggcgtggact accagggtat
      241 ctaatcctgt ttgctcccca cgctttcgag cctcagcgtc agttacagac cagagagccg
      301 ccttcgccac tggtgttcct ccatatatct acgcatttca ccgctacaca tggaattcca
      361 ctctcctctt ctgcactcaa gtctcccagt ttccaatgac cctccccggt tgagccgggg
      421 gctttcacat cagacttaag aaaccgcctg cgctcgcttt acgcccaata aatccggaca
      481 acgcttgcca cctacgtatt accgcggctg ctggcacgta gttagccgtg gctttctggt
      541 tagataccgt caagggatga acagttactc tcatccttgt tcttctctaa caacagagtt
      601 ttacgatccg aaaaccttct tcactcacgc ggcgttgctc ggtcagactt tcgtccattg
      661 ccgaagattc cctactgctg cctcccgtag gagtttgggc cgtgtctcag tcccaatgtg
      721 gccgatcacc ctctcaggtc ggcta
//
LOCUS       DAJSKS010000311          738 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJSKS010000311 DAJSKS010000000
VERSION     DAJSKS010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.322"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            142..>738
                     /locus_tag="ON396_002920"
     CDS             142..>738
                     /locus_tag="ON396_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR3114935.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALG"
BASE COUNT          255 a          109 c          123 g          251 t
ORIGIN      
        1 tctcagggca attctaagaa cgtttcaaga gatagcgaca ctatcgttat cgaaaaaggc
       61 gaaaacctat ctcattttct gctaatgtaa gttggtttag cttttgttaa tatgacttta
      121 tgaaaatgga ggtaattaaa tatgattttt cctaaagtta atgaagcaag cttgaataaa
      181 ggatcctttt ttcatatcta tgaaaacgaa gattttgaac tgtataatat cctttcacaa
      241 aaagaggtca tcgaaaacca aataatctgc catccaatta tttacaaatc tattttcaag
      301 tatgcatttt ttgcttccaa tgattttcaa agaatggaga ttaccgactg tatattcgaa
      361 aactgtgatt tttcaggctg tatattgaca aaaagcatgt tgcatcgcgt gaaatttgtc
      421 aattgtaaat taacaggtac taaattgatg gaaagttata ttgggaacac gttattcgag
      481 aattgtaaaa tggactatgt aaatttatct ggttcaaaca taaaagaaag tacctttgag
      541 cactctgtct tatcaagcgc agattttgta gactgttccc tcactaaaat aatgttttct
      601 actaatgatt tagaaaatgc tagctttttt gatactgatt taaaaaatat cgatttaagt
      661 agcaactcct ttgaaatgat tgaagtacgc tcggaacatc taaaaaattg tacgcttaat
      721 cagttgcaag cacttggt
//
LOCUS       DAJSKS010000312          728 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJSKS010000312 DAJSKS010000000
VERSION     DAJSKS010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.323"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            73..690
                     /locus_tag="ON396_002921"
     CDS             73..690
                     /locus_tag="ON396_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114936.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          262 a           99 c          139 g          228 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccgagaa ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt
      121 ttaaagtgga aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt
      181 atttttactg tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa
      241 ggtcgtttag ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc
      301 ctatatgact atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa
      361 gtaaaagttg gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc
      421 tatacagtag aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga
      481 acagttgtga aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct
      541 attaaaggta cggggaaaag aactatagtt gaggtttcat ctacgtatcc aactacgatg
      601 attctatctt tattaattag ttcaataact tttatattct tatttacacg ttatttaata
      661 agatgctgcg gtgaaatgaa aatagattag atagatatta taaatatgtt gattctattt
      721 tcatagca
//
LOCUS       DAJSKS010000313          716 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJSKS010000313 DAJSKS010000000
VERSION     DAJSKS010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.324"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            319..>716
                     /locus_tag="ON396_002922"
     CDS             319..>716
                     /locus_tag="ON396_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCR3114937.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWV"
BASE COUNT          233 a          137 c          116 g          230 t
ORIGIN      
        1 gttgttaggt gattcatcta gtaaaaacac cagatttcaa atttcacaca atttaaacta
       61 gatcaatcat caaccttctt caaattgtta gaaccctttc ccgaggagct gcagcataag
      121 caaaaaaaca aacaggaagc caaatggcta agacattttt tctattagtc tagttgtttc
      181 tcgtcgcaag tttaagactt tagaaagtta tttctaatga tgtttgggtt gttttgatca
      241 atgcttatgc acagctcctt acctattatt tatctcaaca gatctaacaa attaatggac
      301 tatttaagga gtttttttat gcttacgatc tattcttctc gcacacatag ttaccatctg
      361 atggcaaact ggctgaaaga ccatcaaatg ccttacagag aacgaatttt cacacctaaa
      421 cagcctttaa caaggaaaga gatattttat ttattgtcat taacagaaaa cggatttgac
      481 gatctattag ctaaacgctc gattgattat aaaaatttga aagaccaaat cgaccaatgt
      541 acgacctcag agcttgtcga tctgatcgtc tcgcatttta ctttgttaca acgtcccatc
      601 attactgacg gaaagaaatt attagtcgga ttcaatgaag atagcatgcg tctttttatc
      661 cctgtacaat ggcgcagaag tcaactgaaa tctgtctata acgattttac atgggt
//
LOCUS       DAJSKS010000314          709 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJSKS010000314 DAJSKS010000000
VERSION     DAJSKS010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.325"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            132..344
                     /locus_tag="ON396_002923"
     CDS             132..344
                     /locus_tag="ON396_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114938.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>709
                     /locus_tag="ON396_002924"
     CDS             506..>709
                     /locus_tag="ON396_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR3114939.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMY"
BASE COUNT          254 a          104 c          123 g          228 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtat
//
LOCUS       DAJSKS010000315          693 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJSKS010000315 DAJSKS010000000
VERSION     DAJSKS010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.326"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            93..461
                     /locus_tag="ON396_002925"
     CDS             93..461
                     /locus_tag="ON396_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HCR3114940.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT          242 a          116 c          103 g          232 t
ORIGIN      
        1 ttacaacatt acttgtgttt tgttcgtttt ttatacataa ctattgtttt tctggtaaaa
       61 taaatcaaac cacttaatgg aggcgtttta gaatggaaaa atttcagccg atcatcaatc
      121 aaatcaaaga aaaagaacat caacaaaaga tcaaagacct ttttgctaag ataacagaaa
      181 cgtatcccca gctagacaca actgtaaaat ggaaccaacc tatgtttact gaccatggta
      241 cctttattat tgcatttagt tttgctaaaa atcatttttc tatcgcaccg gaaaaagcag
      301 cgattcgtgc ccttgaaaaa aatatccaag aagcaggcta cgtatatacg gataacgtca
      361 tcaaagttcc ttggaaaagt gtaatcaact gggaattgat tgaacaattg atttcattta
      421 atattgaaga taaaaaaggt catgataaat tttggcgata aagctttata cgaataaacc
      481 ttttactggc gaggaaacta ttcatcacct tatacaggtt ttgttcaatc ctatcctttt
      541 tatcatccct ggttaaacac ttaattgaca aagtatctgc tatttttata aggagtcttt
      601 cttgtgctga aaaaaatcac tgattgaatg gctttgaatt ttctacttag taaaattatt
      661 ttttcagcaa ttttcctgca aaacttttga ata
//
LOCUS       DAJSKS010000316          672 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJSKS010000316 DAJSKS010000000
VERSION     DAJSKS010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.327"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            58..297
                     /locus_tag="ON396_002926"
     CDS             58..297
                     /locus_tag="ON396_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114941.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          221 a          126 c          106 g          219 t
ORIGIN      
        1 ttgcctaata ctgagcatag gtgtatgcta aacgatacta taaggagtgt gtacataatg
       61 aaaagaattc tgtttatcag tttatcaaat ttattgttac ttactgcttg tgggacttct
      121 gcatcaaaga acataccttc agatacgcaa atttcgaata gtatcgattc aacaatttct
      181 acaaatgaga aaaattctcc ggtagaaact tccgatataa aagtttctac ctctgcaaaa
      241 aacagggtaa agaaactgtc agcacgtctc ctttatttca aacaaactct gaattagctt
      301 cttcatcttc ttcagcaaat aatgatgata gtttcgcgtt tactcctaac taaatgcaac
      361 ctttacaaat gctttacgat aacaagcctg agctaaatga tcctgatatc agttttgcat
      421 tttttgaaat gattggatcg gatgatcttt ttaaaactac tagtttgtct atccgaaaat
      481 aggacagttc tggcagtata ggtttttatc gagtttctcc accaggcctc gttacagaga
      541 cagattctct aggcgaacct tttgaataaa tcaaaaatca tgaacaatca ctgccaacta
      601 aataaagtgc cttcaaaagc ttggtcttct cgcttttgaa ggcactttat ttaaatcttt
      661 atgaaactaa ag
//
LOCUS       DAJSKS010000317          662 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJSKS010000317 DAJSKS010000000
VERSION     DAJSKS010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.328"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..208)
                     /locus_tag="ON396_002927"
     CDS             complement(<1..208)
                     /locus_tag="ON396_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114942.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNE"
     gene            complement(215..>662)
                     /locus_tag="ON396_002928"
     CDS             complement(215..>662)
                     /locus_tag="ON396_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR3114943.1"
                     /translation="NTVDLEIKNKLVDELTFYFDTVGFLDEHGVYHIKDFGAIRERIS
                     QDNEQGRAARVFYEHIIVPRMEKVDWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALK
                     AFNFEKAADLLLKIAEKQLGKAAAKELAKVNIVAGIAAAAASCALE"
BASE COUNT          216 a          144 c           73 g          229 t
ORIGIN      
        1 tttcattgac tataactagt tcatctggta aaaaaattaa ccaacaaaca tcgacaaaca
       61 gtaaatgata taatccattt ccacctccaa gcatattcaa tactggtgaa catgccttag
      121 aaacacgaat atagttatgg tttaaataga tattttcttt aaacattgct tgtttaaaag
      181 ccatatgctc ttgttctaaa cttttcataa acttttactc taatgcacag ctagcagcag
      241 ctgctgcaat tcctgctaca atatttacct tagctaactc tttagctgca gcttttccta
      301 attgtttttc tgcaattttt aataataaat ccgcagcttt ttcaaaatta aaagctttta
      361 atgcttcaat aaaatctccc tgcgtaccta tagcccaagc cactcctcct ccaggaagag
      421 aattaatggc tacacatcca gcatatcgtt tccaatcaac tttctccatt ctaggaacaa
      481 taatatgttc ataaaagact cttgctgctc ttccttgttc gttatcttga ctgattcgtt
      541 cacgaatagc accaaaatcc tttatatggt atacaccatg ttcgtctaaa aaaccaactg
      601 tatcaaaata aaaagttaat tcatctacta atttattctt tatttctaaa tcaactgtat
      661 tc
//
LOCUS       DAJSKS010000318          656 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJSKS010000318 DAJSKS010000000
VERSION     DAJSKS010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.329"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ON396_002929"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ON396_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR3114944.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..>656)
                     /locus_tag="ON396_002930"
     CDS             complement(481..>656)
                     /locus_tag="ON396_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114945.1"
                     /translation="VPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNYI
                     LQAILKELLSNAL"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DAJSKS010000319          652 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJSKS010000319 DAJSKS010000000
VERSION     DAJSKS010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.330"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            62..637
                     /locus_tag="ON396_002931"
     CDS             62..637
                     /locus_tag="ON396_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR3114946.1"
                     /translation="MKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDDN
                     PTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIAN
                     YKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYV
                     MMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          216 a          120 c          127 g          189 t
ORIGIN      
        1 ttttcttcta gtctacacac cttaattttt ctgtctagtt aattgtagcc tatccacagg
       61 aatcaaatcg catttatggg taacacaaaa tatttggggc gatcatacag atccttgcgg
      121 ttatctttct gaaatgggtg taagtaaaga aaaactagcc tatgatttgg ctcatggttt
      181 tacggatgat aatccaacta cttcggatga taatccaact acttcggagg ataaaccagt
      241 aattgatcca actcgagcag gtgctgcaaa ttctacgctg acagatggaa caaattacgc
      301 ccacgttgat cagttcggag aaatcgaaaa cgcaaacttg catgtagctg gatggcacat
      361 tgctaattat aaatacgagt atattttcat tatggactac aatactggaa aagaactagc
      421 tagagtaaga gctgatggga tttatagacc agacgtaaat caagcttata atactttagg
      481 aaatgttggc tatcatgtat ctttcaatat gcgtgatttc cctaataaga aagtatacgt
      541 catgatgcgt gcgacaaacg atccagaagg aaatactaag ggtggagcac aagattttca
      601 tgacaagcgt tggtatttaa atattccaca acgataaaaa attcccccct ct
//
LOCUS       DAJSKS010000320          650 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJSKS010000320 DAJSKS010000000
VERSION     DAJSKS010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.331"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
BASE COUNT          202 a          100 c          102 g          246 t
ORIGIN      
        1 gatcgtgtaa tcggtaaagt aattttatta attatacggg atgtaatcaa ttagagaata
       61 aactaatcat aaaaggtact tttacattca taattgtcgc tttgagattc tactttggtt
      121 cactatctga cccaattgca cattttcgat cattacaagt attggtgtcg ttagaacaac
      181 taaataaaaa ctagcggtag atttttcaga atctatgcta atttctgtat ggatgttcca
      241 gtgatggcaa atttctcttt tcattgtttt tcttgtcgca tgatatgcct ttttgtgtta
      301 ttcagttaaa ctatagaaat tctttcctag acagatcttg atcccaaatt ttacgttttt
      361 gagcatcgat atagtttaaa atgacattac tcatatctaa agagtttaga ttttttagga
      421 ctaaaactgt cagttgttct ttttcttctt tctgttaaag tattgtcgcg acttttcgta
      481 tacgttaaga tatgtcgtga aaaaacaagt tttgtgacac aaaaaaacga atgacttgag
      541 gaaatcacct caatcattcg tttaaaatgc tatatattct ttagtttaag atacggaaat
      601 taatgccatc gaccacacta gtagaatgaa cgacatttac ttggcctttt
//
LOCUS       DAJSKS010000321          635 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJSKS010000321 DAJSKS010000000
VERSION     DAJSKS010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.332"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(227..592)
                     /locus_tag="ON396_002932"
     CDS             complement(227..592)
                     /locus_tag="ON396_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114947.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          268 a           76 c          121 g          170 t
ORIGIN      
        1 agaagaaaag aagtaaaaaa tagaaaaact agtgatagtt ttggtgatag tgatattaaa
       61 aatcagaaaa gatagaaaaa gcaggagata gataaaatgc tgatatttca gcatttttta
      121 agagtataaa agagaaaaaa agtaataaat gtatacatga tataagaaaa acatatatgt
      181 ataaaaagaa gaaagtagct tctctttgta catatatggt ttgtttttat ggatgccgag
      241 cttttttata agataaggct accgcccata acgacccaag gatttgtgga actaagtaaa
      301 tacctgcaag aagcaagatc agctgggtcg gttttaaagg atgatagaag taacgccatt
      361 cagtaaaatt ggcgcaaaat aaataggaag tgacagtaga tataatattg gcaatgaata
      421 aagatataat acgcctgttc ctttgaaaat aatatccagc aaaaagagat aaaaatatca
      481 atgtgatgaa cccgcttacg gaatgataac gaaaatcaag cagtaaggcg aaatgcacat
      541 aaggaacaaa agataaaagc aacggaaggt ttttccagca agtttttatc atctttcatc
      601 accacctaac aagtgttatt attttgttag tttta
//
LOCUS       DAJSKS010000322          634 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJSKS010000322 DAJSKS010000000
VERSION     DAJSKS010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.333"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            74..>634
                     /locus_tag="ON396_002933"
     CDS             74..>634
                     /locus_tag="ON396_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3114948.1"
                     /translation="MKIAYIRVSSIDQNEQRQIEEMKKFGAEKIFIEKQSGATITHRP
                     IFQEALEFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PHLDKFIKDLIIQILAMIAEQERTESKRRQAQVIKIAKANGVYKGRPKLYSADTKNPQ
                     RRLVYENIVKDLNEGIAISKIAKNYNI"
BASE COUNT          233 a          105 c          119 g          177 t
ORIGIN      
        1 ctttatagtg tataaaaaaa ttagtgttga ttttgagtgt attttaaata gtttgaggaa
       61 gtaggtaaaa attttgaaaa ttgcttatat tcgagtttcg tcgattgatc aaaatgagca
      121 acgccaaatt gaagaaatga aaaagttcgg cgcagaaaaa attttcatcg agaaacaatc
      181 tggagcaacg attactcatc gaccgatttt ccaggaagct ttagagtttg tccgagacca
      241 agatattttt attgtcgaag caatcgaccg acttggaaga aattacgacg aaatcattgc
      301 ttcagtcaat tacctaaaga agaaaaacgt gcagctgatc atcaccagtc taccgatcat
      361 ggctgaagca attggcaatc ctcatttgga taaattcatt aaagatttaa tcatccaaat
      421 tttggccatg attgcagagc aagagcgtac cgaatctaaa cgacgccagg cgcaagtcat
      481 taaaattgca aaagcaaatg gcgtctataa aggtcgtcca aaactctata gtgcagatac
      541 aaaaaatcct caacgacgtt tagtttatga aaacatcgtt aaggatttaa atgagggcat
      601 agctatttca aaaattgcta aaaactacaa tatt
//
LOCUS       DAJSKS010000323          602 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJSKS010000323 DAJSKS010000000
VERSION     DAJSKS010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.334"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(52..246)
                     /locus_tag="ON396_002934"
     CDS             complement(52..246)
                     /locus_tag="ON396_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114949.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(346..432)
                     /locus_tag="ON396_002935"
                     /pseudo
     CDS             complement(346..432)
                     /locus_tag="ON396_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          203 a          106 c           92 g          201 t
ORIGIN      
        1 tgtatatgtc atttaggtat ttcagttatg ttataatagt tttgctgtct gctaccccgg
       61 cctaatctgg caaggggagg aggagatata atgaaatacc tttttgattt tgtttttgca
      121 atcaagccac ttgcagatgt agtgtgcagt tacctctgca aatggcttga ttgcaaaatt
      181 gccaaaaaag acagtaagcc tacccgttaa ttctataata tagaaacgta aaaaaaaccc
      241 catcagcctg cacgctgatg gggtttggtg atataatgaa atacctcttt gttactcata
      301 attataccat aattagtcac gaatgcaacc aatagaaaaa actacttaga acggtagatc
      361 atcgctaata acaatgcatt gtccaccaaa tggattttgt gaagcattaa atacattgtt
      421 attaattgtc gctgctttta actaggcctc tccccttcta ttataaaaga agatatatat
      481 catttcttaa ttacctacta ccttaaacca aaatttaagt actacctaat ttaagtagta
      541 cttaaacaat ctaaaaaata gttatcccaa ttgttatgct tttataatac gattatttgt
      601 aa
//
LOCUS       DAJSKS010000324          597 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJSKS010000324 DAJSKS010000000
VERSION     DAJSKS010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.335"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..409)
                     /locus_tag="ON396_002936"
     CDS             complement(<1..409)
                     /locus_tag="ON396_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114950.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRN"
BASE COUNT          173 a          111 c           86 g          227 t
ORIGIN      
        1 gatttctttt tagtctttgt aatactttat aagccacaga ataactcacg tgatgctgtt
       61 tagcaaaatt ttctactgag atatattttt cctgaaagaa agccaatacc attttcatag
      121 ataaagaatc cgttatataa cgagataaca gagctttgct agaacatttt ccattaattt
      181 ccagacatat atccaattca ctgacggtta ctcgcaaatt tttctctaga tcgaactttt
      241 gtaaatcatg tttcagctta tcgatcgttt ttttcaagag aaatgtcgat agcgacaact
      301 cttcttgaac attcgatatt gactctgata aatttgagct tgcttctaaa tatttaagta
      361 atgaataact tctgtgatct tctttgctta aaaaaaactc ataattcatt agtcatcagc
      421 tctttctacg tatattactt tccatgcatt caccaactag ctggggataa aactgatttc
      481 ctggatccga gtcactgttg tcagcgattc ttgtttgtat aatatacatc gtcctgtctt
      541 ttttttaaca ttttggagag cgttttattt ggcctttctc gcgatcaaac ctgttat
//
LOCUS       DAJSKS010000325          589 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJSKS010000325 DAJSKS010000000
VERSION     DAJSKS010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.336"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            24..>589
                     /locus_tag="ON396_002937"
     CDS             24..>589
                     /locus_tag="ON396_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HCR3114951.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT          209 a          101 c          116 g          163 t
ORIGIN      
        1 aaaagatagc aggaggaatg acgatgaatc attttaaagg aaagcaattt cagcaggatg
       61 tgattattgt agccgtgggc tactatcttc gttataacct tagctatcgt gaagttcaag
      121 aaatcttata tgatcgtggc attaacgttt ctcatacgac gatttatcgt tgggtgcaag
      181 aatatggcaa actactctat caaatttgga aaaagaaaaa taaaaaatcc ttttattcat
      241 ggaaaatgga tgaaacgtac atcaaaatta aaggaaaatg gcattatttg tatcgagcca
      301 tcgatgcaga tggtttaacc ttggatattt ggttacgtaa aaaacgggac acacaagcag
      361 cctatgcttt tcttaagcgg ttagtgaagc agtttgatga accgaaggtt gtagtcacag
      421 ataaagcccc ctctattaca agtgccttta agaaactaaa agaatacggc ttttatcaag
      481 ggacagaaca tcgtaccatt aaatacctga ataatttgat tgaacaagac catcgtccag
      541 taaagagacg caataaattc tatcgaagtt tacgcactgc ctctaccac
//
LOCUS       DAJSKS010000326          575 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJSKS010000326 DAJSKS010000000
VERSION     DAJSKS010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.338"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            10..552
                     /locus_tag="ON396_002938"
     CDS             10..552
                     /locus_tag="ON396_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCR3114952.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
BASE COUNT          177 a           99 c          124 g          175 t
ORIGIN      
        1 gttttacaaa tgattgatcc aaaaatgttg gggatgatct tcctcattaa ttttgcttat
       61 atcactctga acacgttacg tttcatgttg acaatgaaag gctatcgact gattgctcca
      121 cttgtcagta tggtcgagat cacgatttat atcgtcggct tatcccttgt tttagatcgt
      181 ttagataatc cactgaattt atttgtctat gcgttaggtt acgcagtagg tatcagtgtc
      241 ggaattttga ttgaagacaa attggcatta ggatacatca tggtcacaac gattttgcca
      301 gcaaatagcg cagaagataa aagtttgccg ggtatactga gagaacacgg ttatggtgtg
      361 acacagactt ctgcaatggg gctggaaggt gagcggctag ttctcgaaat actttctcct
      421 agaaaaagcg aacgtgaact gtatcgcctg atcaaagaaa tcgaagagcg tgcttttatc
      481 atttcttatg agccaaaata tatttcaggt ggtttctgga ctaaaaaagt gagaaaacga
      541 caaggaaaat aactcacaaa cttaaaaact gtcta
//
LOCUS       DAJSKS010000327          570 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJSKS010000327 DAJSKS010000000
VERSION     DAJSKS010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.339"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            48..494
                     /locus_tag="ON396_002939"
     CDS             48..494
                     /locus_tag="ON396_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR3114953.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT          192 a           85 c          119 g          174 t
ORIGIN      
        1 tgtaaaaata gtatgataag acgagaataa caacgtgggg tgtttacatg gaaaataagt
       61 tagtcgaaga ggttatcgaa aagttaaaaa aagagaacat tcgaatcaca cctcaacggt
      121 acgcggtttt agaatatttg attgaacatc attcccatcc aacggctgat gagatttata
      181 aagcacttga acatcggttt ccaaatatga gtgttgcgac tgtttacaat aaccttcgct
      241 tatttacttc gattggattt gttcaagaaa tgaagtacgg cgatgcttct agccgatttg
      301 atttcagttc aaagcgtcat tatcatgtaa tttgtgaaaa ctgcggtaag attgttgatt
      361 ttcattaccc agggcttgaa gatatagaaa tggctgcaag cagactgact gattttgaga
      421 ttaatgaaca tcggttggaa ttgtatggac tctgtccgga ttgtaaagag tcagcaaaaa
      481 aggcgcaaat gtaaaaacag tttctacaag tgttatacct aaaaaaagct agctgtgtaa
      541 aaaaaacgct agctttttta tttttttgtc
//
LOCUS       DAJSKS010000328          568 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJSKS010000328 DAJSKS010000000
VERSION     DAJSKS010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.340"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..>568
                     /locus_tag="ON396_002940"
     CDS             <1..>568
                     /locus_tag="ON396_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR3114954.1"
                     /translation="KGKGRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVRE
                     IRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKL
                     AKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYK
                     EVPKHQHKRALVLTARKFTRLVDTLLRKH"
BASE COUNT          212 a          109 c          103 g          144 t
ORIGIN      
        1 aaaggaaaag gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt
       61 accatgagtt accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt
      121 ctcgtacgag aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa
      181 caagtaattg ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa
      241 gtctatgctg ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca
      301 aaattggcaa aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg
      361 caaaatacac ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct
      421 gccaactctg taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag
      481 gaggttccaa agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt
      541 ctggtggata cgctactacg taaacacc
//
LOCUS       DAJSKS010000329          559 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJSKS010000329 DAJSKS010000000
VERSION     DAJSKS010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.341"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(48..121)
                     /locus_tag="ON396_002941"
     tRNA            complement(48..121)
                     /locus_tag="ON396_002941"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(85..87),aa:Ile,seq:cat)
BASE COUNT          194 a           91 c          100 g          174 t
ORIGIN      
        1 actcttcaaa aaattctaac acagaacgat tcaattggct acattaatgt accctgtagg
       61 actcgaacct acgaccggac ggttatgagc cgtctgctct gaccaactga gctaagggta
      121 gtggttgttg ccacataaag ccataaacaa tcaaccagta gaatgcgtgg caacaaacct
      181 gttatcgcat atcttggagt gtgactattt atgagtgata gtgaagatat gcgtctccat
      241 atatgtatgg ttgagacaga taaacatgta tgaaaaacgt tgttaaatca acgttttttt
      301 attttggtca aggagagaaa agtagagaaa tacaaaattt tttgcattga ttttgcattc
      361 aaaaaaacat tcaaacattt agaaatcccc aatacgtatt caggaaaatc tattttaagg
      421 tttacaaaaa gaacttttgt tcgtacactc tttttgaggt gaacttttat gctgatggaa
      481 ggaaaaacac aattatggtt taagcatgtt ccttcaaata agttttttaa ttataaactc
      541 tagagaaatt aattcatcc
//
LOCUS       DAJSKS010000330          549 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJSKS010000330 DAJSKS010000000
VERSION     DAJSKS010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.342"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            124..522
                     /gene="spxA"
                     /locus_tag="ON396_002942"
     CDS             124..522
                     /gene="spxA"
                     /locus_tag="ON396_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCR3114955.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          195 a           87 c          110 g          157 t
ORIGIN      
        1 aaaaaattat gaaaaaaagg tagctattta aaagttgatt atgtataata gaaacagatg
       61 ggagattatt atgatcttct gttacaaaaa agtgattctt atcacgaaaa ggagtggtgc
      121 acgttgttaa cattatatac ttccccaagt tgtacttcct gcagaaaggc tcgtgcgtgg
      181 ttgcaagaac atcagattcc atttgttgaa cgtaatattt tttcagaacc attgaacagt
      241 tctgaattaa aagcgatttt acaaatgaca gaagacggaa cagaagaaat catctcgaca
      301 cgttccaaag tattccaaaa actgaacatg gatttagatg atctgccgtt acaagaatta
      361 ttggaacttg tgcaaaacaa tcctggattg cttcgccgtc caatcatgat tgatgataaa
      421 cgtctgcaag ttggttttaa tgaagatgaa attcgtcggt tcctaccgcg cgatgtacga
      481 caattagaat tacgtcaagc acaattgatg gctggattat agaaggataa tacgaaactt
      541 ggaaaagac
//
LOCUS       DAJSKS010000331          542 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJSKS010000331 DAJSKS010000000
VERSION     DAJSKS010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.343"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(1..542)
                     /locus_tag="ON396_002943"
                     /pseudo
     CDS             complement(1..542)
                     /locus_tag="ON396_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          191 a           84 c           87 g          180 t
ORIGIN      
        1 ggtcaagtac ttctttttta aaaaaagtat tcatcataaa agccactact tgcggatctt
       61 catcataaaa ttctgtagca gtacttgaaa agatgttcca ataaatatct aatgctgcat
      121 attgaatttg tacctctttt ccagaaaaag actttctgcc attattagaa aaggagatag
      181 acaaaccgta ctatgctaaa aatgcattaa tctttttcat tttcgtatac aaataagagg
      241 cactatagtt tgtttgctga ctgaaactga taatcgaatt tgtctgacag ctgaacaaag
      301 aagtcaagat ctgataactg actgattcgt tgcagtatct tccaaataga cgataatagc
      361 agtaagccgg atcaattcct tcaaaattca actttaaaaa aggtttttgc ttatcaatat
      421 gcagttctgt tagattgaga cgctcaaagt cttgttctaa ttctttgatg tatagtaaaa
      481 aatttgattc agacatatgg aaatgatctg ctaaatattg tgtagtaata aagaaacgat
      541 gt
//
LOCUS       DAJSKS010000332          515 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJSKS010000332 DAJSKS010000000
VERSION     DAJSKS010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.344"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            100..>515
                     /locus_tag="ON396_002944"
     CDS             100..>515
                     /locus_tag="ON396_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HCR3114956.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYEL"
BASE COUNT          200 a           59 c           94 g          162 t
ORIGIN      
        1 gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact
       61 tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt
      121 ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca
      181 agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta
      241 gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat
      301 ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat
      361 gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga
      421 agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt
      481 gaattttcta tcaaagagct aaactatgag cttga
//
LOCUS       DAJSKS010000333          512 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJSKS010000333 DAJSKS010000000
VERSION     DAJSKS010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.345"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..343
                     /locus_tag="ON396_002945"
     CDS             <1..343
                     /locus_tag="ON396_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114957.1"
                     /translation="DNIRDKKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDL
                     IRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRA
                     SLYRKLSEVNN"
BASE COUNT          206 a           69 c           93 g          144 t
ORIGIN      
        1 cgataacata cgagataaaa aggcaagttt aaaatcacta aaagatacat ggcttgattt
       61 atcagaagat aatccataca gccaattctt aattactgta atggctggtg ttaaccaatt
      121 agagcgagat cttattcgga tgagacaacg tgaagggatt gaattggcta agaaagaagg
      181 aaagtttaaa ggtcgattaa agaagtatca taaaaatcac gcaggaatga attatgcggt
      241 aaagctatat aaagaaggaa atatgactgt aaatcaaatt tgtgaaatta ctaatgtatc
      301 tagggcttca ttatacagga aattatcaga agtgaataat tagccattct gtattccgct
      361 aatgggcaat atttttaaag aagaaaagga aactataaaa tattaacagc ctcctagcga
      421 tgccgaaaag ccctttgata aaaaaagaat catcatctta agaaattctt agtcatttat
      481 tatgtaaatg cttataaatt cggccctata at
//
LOCUS       DAJSKS010000334          510 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJSKS010000334 DAJSKS010000000
VERSION     DAJSKS010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.346"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(103..303)
                     /locus_tag="ON396_002946"
     CDS             complement(103..303)
                     /locus_tag="ON396_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR3114958.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          179 a           80 c           87 g          164 t
ORIGIN      
        1 ctattatgat aacatttctt ataataacaa acacaacaga agacgtgtaa tagttagtca
       61 ggaacgtaca aataacccct gtatcctttg cggtacaggg ggttattttg tataagtaga
      121 cattgtagtt tctaaactgc tatgtcctaa acgtttcgat acgctaagta tatttactcc
      181 ttgataaagt aaaatagatg catgcgtatg tcttagtcca tcaataaaca gtcgaagaga
      241 aaatagggaa aaattgatta ctaaagaaaa acttactagg tcgacggtgt agttgatttt
      301 catacttcaa tagattcgaa ttaaacgatt tcgattggct tataccagtc ctatccaacg
      361 aaagatatat cgttcttaac tctagcggta taacttgaag ctttacgtaa agccaatggg
      421 aatgaactgg agatgacttt ttattatttt tagtaatagc tgatgggtta atacttacaa
      481 ctttcctttt caaagtacca tataaaaaaa
//
LOCUS       DAJSKS010000335          508 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJSKS010000335 DAJSKS010000000
VERSION     DAJSKS010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.347"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..461
                     /locus_tag="ON396_002947"
     CDS             <1..461
                     /locus_tag="ON396_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3114959.1"
                     /translation="DLDALQVVSVVSFYESFIDGADPLDMQGYLQTQLTTEILAVELA
                     QSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLGQRLLDEANRYAKT
                     KQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPILEKEI"
BASE COUNT          179 a           73 c           98 g          158 t
ORIGIN      
        1 atgatctaga tgctttacaa gttgtaagtg ttgtcagttt ttatgaatcg tttatcgatg
       61 gcgcagatcc acttgatatg caaggatatt tacagacaca attgacaact gaaatattgg
      121 cagtagaatt ggcacagtca accagtaaat ttattggtat taaggatcat caaatactca
      181 ttggttatat gaaagtcaat gatgaaaaag atgctataga gattcaacgg ctctatctgc
      241 tcaaagatta tcaaaacaaa gggttagggc aacggttact cgatgaagcg aatcgttatg
      301 ctaaaacgaa acaaaaacgt tatttacgtt tgactgttta tgaaaaaaat catgcaggta
      361 ttcgttttta tgaacgtcat ggatttaaaa aaatcgggat caaacatttt cctttaggaa
      421 aacaagatcg tatttgtccg attttagaaa aagaaatcta aaaaaacgtc ctcacttttg
      481 atgctgagga cgttttttta gactggta
//
LOCUS       DAJSKS010000336          494 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJSKS010000336 DAJSKS010000000
VERSION     DAJSKS010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.348"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            1..268
                     /locus_tag="ON396_002948"
                     /pseudo
     CDS             1..268
                     /locus_tag="ON396_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
BASE COUNT          184 a           73 c           87 g          150 t
ORIGIN      
        1 atctatattt tgatatacaa attacagctt cagatactaa gttgcataaa ccttttccgg
       61 accccataca aaaggcaatt gatgaattgg gcataaaaaa taagaaacga tttatattgg
      121 tgattcatta tatgacatgc aaagtgcgaa agcttgtggg atagcatttg gtttagctaa
      181 atggggagcc aaaaatatag aaaattttca aagtgtagat ataattattg attctcctca
      241 agaaatactt gatttgttag aaaaatagac aaagagcgtg agataaggat agggtctcac
      301 gctctttgtt tttcgccatt taaagattga aatttttgcc atcgccgatt gtaaaattaa
      361 gctagacaac taaaaaagtc atttgtgcta cactcaaaat agttccgacc aaagaattat
      421 aaggagtgag tacaaatgac ctatacccat cttacaacgg atgaacttgt aataatagag
      481 tcttatttca aaat
//
LOCUS       DAJSKS010000337          479 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJSKS010000337 DAJSKS010000000
VERSION     DAJSKS010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.349"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(93..392)
                     /locus_tag="ON396_002949"
     CDS             complement(93..392)
                     /locus_tag="ON396_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR3114960.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          148 a           96 c           56 g          179 t
ORIGIN      
        1 aaaaaaatac agactctgtt caaataaatg aacctagcct gcattatgag attgactata
       61 ctttttaatt cattgagatg aataactcgt ttttataaat gcccaaaagt aatcggtgcc
      121 gaagccattc ctcgacctaa ttctttttca aattttccta ttgttgatat ctccatataa
      181 aacggtgata ctggtttaga caattctccc ctgattgcta acggacgtaa actacgttcc
      241 aaattattta tgactttagg ataatactga cctttttcta agcctgtctt tgcgtctatc
      301 aaaagtatac gttccttttc tttgatctct ggatttaaaa cgagatcata taattttagt
      361 accatgtttt ctatttcttc acttttagac atcacgattt cctcccaaaa atatatttct
      421 tcccactaca ttctataaac aaagtatacg ctacatctag atttttttaa atcattact
//
LOCUS       DAJSKS010000338          456 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJSKS010000338 DAJSKS010000000
VERSION     DAJSKS010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.350"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(14..400)
                     /locus_tag="ON396_002950"
     CDS             complement(14..400)
                     /locus_tag="ON396_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114961.1"
                     /translation="MKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRFEVLKIQKNE
                     LLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTDVVMITDIYV
                     EDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          137 a           89 c           49 g          181 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg ttcctttatt tttattaaca agttttatca
       61 caggcttcat atatttgatt atatcttcat cttctacata gatatctgtt atcattacga
      121 catctgtact atttcgattg agtactttca cttgatctcc agcattgatt ttccctctaa
      181 atcttttagg aatgaaccaa ctatacatat gattagcatc accaaaatgt gcttttatcg
      241 cattctcagg atataactta ttcaatagtt cattcttttg aattttcaaa acttcaaatc
      301 tatacttatc atcttctgct aatctaaata aatcttctaa acgcatgttt tctaactgta
      361 agttttgaaa atcttccggt ataaattttc tatatttcat taataatgaa cgcccagccc
      421 gatttattag aatctggcca ttttggtcat ctctat
//
LOCUS       DAJSKS010000339          454 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJSKS010000339 DAJSKS010000000
VERSION     DAJSKS010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.351"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(194..>454)
                     /locus_tag="ON396_002951"
     CDS             complement(194..>454)
                     /locus_tag="ON396_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3114962.1"
                     /translation="PKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVR
                     RREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          158 a           59 c           80 g          157 t
ORIGIN      
        1 tattaaacag gaatttatcg aaaatggtag aaaagaaatt tataaaagga cttataaaga
       61 ttgattatat aatatatgaa aagctattat aaaagacatt agtattaaat agtttaaaaa
      121 aatgaaaaat aataaaggaa gtgagtcaat tccagactcc tgtgtaaaat gctatacaat
      181 gtttttacca tttctactta tcaaaattga tgtattttct tgaagaataa atccattcat
      241 catgtaggtc cataagaacg gctccaatta agcgattggc tgatgtttga ttggggaaga
      301 tgcgaataat cttttctctt ctgcgtactt cttgattcag tcgttcaatt agattggtac
      361 tctttagtcg attgtgggaa tttccttgta cggtatattg aaaggcgtct tcgaatccat
      421 catccaatga tgcgcaagct tttgaatatt ttgg
//
LOCUS       DAJSKS010000340          450 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJSKS010000340 DAJSKS010000000
VERSION     DAJSKS010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.352"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(102..>450)
                     /locus_tag="ON396_002952"
     CDS             complement(102..>450)
                     /locus_tag="ON396_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114963.1"
                     /translation="FADSTNEPTTVVDNNQELTPLDRNGIAIGNPTNDLTVTENYLIV
                     SPLLARAASKTIRVTGTMIFDKVGWPSRPPQSRNWKEKRNGYWYYGKLYLQSYKSAGN
                     NWIATYSGTLTRK"
BASE COUNT          134 a           80 c           70 g          166 t
ORIGIN      
        1 acttgtaaag ctgattcttc tttcggtaaa ttaattttac ttagaatatc gctatcttga
       61 taaaaatttg gtgtgttcac aaattctggc ataaaaaatc cctactttct agttaaggtt
      121 cctgaataag tcgcaatcca attgtttcca gctgatttat aactttgtaa atacaatttt
      181 ccatagtacc aataaccatt tcttttttct ttccaattcc gtgattgagg aggtctagaa
      241 ggccaaccaa ctttatcaaa aatcatcgtt cctgtcactc gtatcgtttt agaagctgct
      301 cgagcaagta atggagaaac tattaaataa ttttcagtaa cagttaagtc gttagttggg
      361 ttaccaatgg ctattccgtt cctatctaaa ggagttaatt cttggttatt atctactaca
      421 gtagttggtt catttgtact atcagcaaat
//
LOCUS       DAJSKS010000341          445 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJSKS010000341 DAJSKS010000000
VERSION     DAJSKS010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.353"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
BASE COUNT          140 a           87 c           52 g          166 t
ORIGIN      
        1 aaaaaattat tacgttatta aaaaaacaga tgaaagatca ggttttatta gatctcattc
       61 tttcatcact tgaagggatt taatgaacta ctttttcctc tttgtcgttt ttatatattc
      121 gtattccttc aaacagtagg atagcctctt tcaatgcttg atatttgaac tgtccgttac
      181 ttaaaaattt agatgtgccg ccctcttcct agaacatatt cttttttact ttttcattaa
      241 ttgacagacc aatcgaacga aactgtaaat ttcgatagga taatgattgg tcaaactcat
      301 tcattttcaa gacatctcga ttcatattca agataggaat ctttttatca acatcacttt
      361 attcgtattt tcctctacca taaaagcttc acgctccaca aaattgtttt atacactact
      421 attcagccca ttcaacgacc aatga
//
LOCUS       DAJSKS010000342          445 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJSKS010000342 DAJSKS010000000
VERSION     DAJSKS010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.354"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
BASE COUNT          154 a           59 c           78 g          154 t
ORIGIN      
        1 actgtagaca tcatccataa aatggacttt gtctacagtc tggataacgt gattttttgc
       61 tgtaatgaca aggttttagc tgtttcagat accatgtatc caaaaatagt ttattgagaa
      121 aataggagag tatttgatac taactagtag gaaaaaatga gataaaaagt aaatggaatc
      181 atttaaggta atctgaaata aaaaatatac agccctgtgg tactttacct acaaggctgt
      241 atatattttt tactctcagc gtttttgact ccgtgtctga ctccgtttta ttgattttga
      301 atgttattca atgaccattt aacaggtcaa atttgatgtt tttgattccg tatgacgccg
      361 tttgatatct agtgatatag tgatttgagt ctacatcgaa taagggaata aaccaataaa
      421 aaatgattta gaatattaaa ataac
//
LOCUS       DAJSKS010000343          426 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJSKS010000343 DAJSKS010000000
VERSION     DAJSKS010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.355"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..>426
                     /locus_tag="ON396_002953"
     CDS             <1..>426
                     /locus_tag="ON396_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR3114964.1"
                     /translation="KISYQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPW
                     IIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTI
                     IGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDN"
BASE COUNT          148 a           53 c           80 g          145 t
ORIGIN      
        1 aaaaaatttc ttatcaatac catgtaggac agtatttacc cgactggcat ccttgggaag
       61 attatcgcaa tttttttgtt tcaaacaaaa aaactaatgg gtgtagagaa atattcgcta
      121 ttgaattacc ttggattata gatacatttg ggaaagttaa atcgtttact gtaatgaccc
      181 agaaattatc caatcttgat attgattatt cggacagttt cactcttatt attgagcatg
      241 aaactggtat agttggaact ttaagtgtag atgttgtttc aagagtagct aaaagaaatt
      301 taactataat tggtgaaaag attttaatag aatgggaagg taacccggat tctctatttt
      361 catggaatga agaaacttct agtatgacag atattcaatt gtacgatgag gttatgcatg
      421 ataata
//
LOCUS       DAJSKS010000344          421 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJSKS010000344 DAJSKS010000000
VERSION     DAJSKS010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.356"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            55..249
                     /locus_tag="ON396_002954"
     CDS             55..249
                     /locus_tag="ON396_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114965.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          179 a           56 c           77 g          109 t
ORIGIN      
        1 tcacttacat atatttgaat attaactaaa aacagcaaga aagaagagga tcaaatgtat
       61 tatgtagaag taaaaacaaa aggcgtcaaa aacaaacaat atgtgaaagg tatcagtaac
      121 gaatatcctt tacttggttc atggaaagaa gctgctcctt tttccaaacc atgtgccata
      181 aaaataaaga acgagctaga aaaagaactg acttgtggta aagctgttgt agatatcatc
      241 gaaaagtaaa tccaatgtat agaaaggaag atcacacatg aaaagcgacg tacaaattgc
      301 taagattata gaattagaag aagaagacgc agtgttgaca attgaaaaaa aataaatgtt
      361 tgtataccat atgattattt caatatgtat cctggaattg gagatctcgt agagctatat
      421 c
//
LOCUS       DAJSKS010000345          421 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJSKS010000345 DAJSKS010000000
VERSION     DAJSKS010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.357"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(170..355)
                     /locus_tag="ON396_002955"
     CDS             complement(170..355)
                     /locus_tag="ON396_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HCR3114966.1"
                     /translation="MQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRA
                     WRKMLDDELIPLENELR"
BASE COUNT          120 a           85 c           62 g          154 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagaggtt
      361 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
      421 a
//
LOCUS       DAJSKS010000346          416 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJSKS010000346 DAJSKS010000000
VERSION     DAJSKS010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.358"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..236)
                     /locus_tag="ON396_002956"
     CDS             complement(<1..236)
                     /locus_tag="ON396_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114967.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDAR"
BASE COUNT          151 a           67 c           55 g          143 t
ORIGIN      
        1 cgtgcgtctg gcacatcagt cacgatatca aacaaaaaat acacatcttt cttcattctg
       61 gtttttgttg ccggaatttt aaagtagttc ttattagagt aatagagatt gattaataag
      121 ctatcttcga ttgctaaaaa gaaaacttct gaatcccaca ccttataaaa atctttgaca
      181 aatctattcg aaggatcaaa tttaaaccat aattgtgttt ttccttccat caacataatg
      241 ttcacctcaa aaagagtata cgaactaatg ttctttttgt aaagtaggaa tatcattgat
      301 aatatggatg gaacatatat attaaaaagt gttctttttg aatgctattt tgaatgccat
      361 tttcattcat ttaacgaaaa tctgcgaaaa taaaaaatgc tcaaaccctt ataaaa
//
LOCUS       DAJSKS010000347          415 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJSKS010000347 DAJSKS010000000
VERSION     DAJSKS010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.359"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..>415
                     /locus_tag="ON396_002957"
     CDS             <1..>415
                     /locus_tag="ON396_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114968.1"
                     /translation="VSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKL
                     DDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKG
                     TVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          169 a           62 c           95 g           89 t
ORIGIN      
        1 gcgtaagctt accagaagat aaacttccag tgttgaaagc aaaagatggt tatacagatg
       61 cgaaatggcc agaggaagca actcaaccaa ttacagcaga tgatacagaa tttgtatcaa
      121 gtgcaacaaa actggatgat aaatctgacg ctgacaaata taatcctgaa ggtcaaaaag
      181 tgactacaga attgaataag gaaccagacg catttgaggg aattaagaac aaagaagatt
      241 taccaaaaga tactaagtat acttggaaag aaaaagtaga tgttagcgca gctggaaata
      301 aaaaagggac tgttgtagtg acatatccag atggatcatc tgatgaagtt gaagtagatg
      361 ttacagtgac agacaatcgt tctgacgccg ataaatatga gccaacagta gaagg
//
LOCUS       DAJSKS010000348          404 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJSKS010000348 DAJSKS010000000
VERSION     DAJSKS010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.360"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
BASE COUNT          150 a           63 c           66 g          125 t
ORIGIN      
        1 agaaaacctc cagacttctt gatagctcaa gtatatcttg aggtgcattt gttagtaagg
       61 tactgttcta tggggcgctt actctaggag tgagcatttt tcgaaaaata ctaaaaattt
      121 ttcaaaataa aaaaaggcat acagacaata aactgtctat acgcctcaca gactctattc
      181 tatacatcta attttggctg tcctgcaaaa aatatgtgtt agcctccatc gaaagatcaa
      241 actttggaaa acatcattat aactttgctt ggtaagagct ttgctaaaac atgatatgac
      301 ttgtatacaa aatgtggagt gtattccaat aaataggaag gattacaaaa tttgaaacca
      361 aataagtaaa aaatactgtc actttgaatt aatttgaaat agta
//
LOCUS       DAJSKS010000349          398 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJSKS010000349 DAJSKS010000000
VERSION     DAJSKS010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.361"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..323
                     /locus_tag="ON396_002958"
     CDS             <1..323
                     /locus_tag="ON396_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3114969.1"
                     /translation="EALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIY
                     STNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTL
                     ESMI"
BASE COUNT          148 a           73 c           57 g          120 t
ORIGIN      
        1 aagaggcatt ggatcacatc gactttatga ctaggaaatg gaaaaagcag tatccaagag
       61 tcgtcaattt actcttgaat cctgccctat taacctttta taatttccct cacgccatca
      121 gacgaacaat ttattcgacg aacctgattg aaggctttaa taagcagcta aaacgatata
      181 ctcgaagaaa agaacaattc cctaatgaag aatctctaga gagattcttc atttctcaat
      241 ttaatcaata taaccaaaaa tttttaggta gaattcacaa gggatttaaa gaaattcagg
      301 atacattaga gtcgatgatt taactgtaat taacagaatg gattttccat ttacatataa
      361 ttcttgacgc taccataatc taaataacca tcatattc
//
LOCUS       DAJSKS010000350          388 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJSKS010000350 DAJSKS010000000
VERSION     DAJSKS010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.362"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..136)
                     /locus_tag="ON396_002959"
     CDS             complement(<1..136)
                     /locus_tag="ON396_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR3114970.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     K"
     gene            complement(155..316)
                     /locus_tag="ON396_002960"
     CDS             complement(155..316)
                     /locus_tag="ON396_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR3114971.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT          128 a           74 c           41 g          145 t
ORIGIN      
        1 ccttttctat ttcattgctt gatttcatta ttgttaaaca agaatacaag caagaaagaa
       61 ctatagttaa tatgataaaa attattaaag ttctattttt ctttcttgta atatatgcta
      121 tagcattttt tatcactttc ctaactactc ctctctattt tcttactcca taactaattg
      181 catccactgg acaagcattt ttacatcgtc cacatctgat acactccaga tgattgcaat
      241 tctcaactga atctttattc atttggcaaa cttttttaca ttttccacaa ttaatacact
      301 tatccttatc ctgaatcggc tacactagac tagacagaaa aaataaggtg tgtagaataa
      361 taaaaaacac actggaggat ttttcatg
//
LOCUS       DAJSKS010000351          382 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJSKS010000351 DAJSKS010000000
VERSION     DAJSKS010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.363"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..>382
                     /locus_tag="ON396_002961"
     CDS             <1..>382
                     /locus_tag="ON396_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114972.1"
                     /translation="FDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQAVANN
                     IGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIHELAHA
                     RMHKFSELDTATKELQAEMTSYIV"
BASE COUNT          140 a           68 c           76 g           98 t
ORIGIN      
        1 tgtttgatat cacacaaaca cagttaaaac ctgaagacta tccgaaaatc tttccgaacc
       61 gtgtctttga ttttcaattg gataaaaaag gacagtcaga acttcaagct gggattcaag
      121 cggtagccaa caatattgga atacaaattc gagatatgac agaaagtgaa gtctatcaaa
      181 gagaattagg acaagcacgt ggggcgtatg ttcgtaacga atttactggc gaagaagaaa
      241 tcgtcatgaa tactcgaaat actcctacgc agcatttagc tacaagtatt catgaattag
      301 cccatgcaag aatgcacaaa ttttcagaat tagataccgc aactaaagaa ttgcaggcag
      361 aaatgacttc ttatattgtg tg
//
LOCUS       DAJSKS010000352          354 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJSKS010000352 DAJSKS010000000
VERSION     DAJSKS010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.364"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            118..318
                     /locus_tag="ON396_002962"
     CDS             118..318
                     /locus_tag="ON396_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR3114973.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          125 a           56 c           66 g          107 t
ORIGIN      
        1 atctattttg aaacgtaatt tgagcgatat atctacatag ataaaactcc ttttgcctat
       61 caaatattta ttgcaacgaa aaaacgtaca ttgatacgta tcaggaggaa tttatttatg
      121 aataacggta cagtaaaatg gttcaacgca gacaaaggtt ttggctttat cactggtgaa
      181 gatggaaatg atgtatttgc acatttttca gctatccaag gtgacggctt caaaacttta
      241 gaagaaggcc aagcagtgtc ttacgatgtt gaagatggtc aacgtggccc tcaagcaaca
      301 aacattgtta aagcttaata aaaattgact tttaaccgtc ttttcgaaag aaaa
//
LOCUS       DAJSKS010000353          353 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJSKS010000353 DAJSKS010000000
VERSION     DAJSKS010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.365"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..341)
                     /locus_tag="ON396_002963"
     CDS             complement(<1..341)
                     /locus_tag="ON396_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114974.1"
                     /translation="MDPAIVIPQYFAQLSEALVVIEADVFAHFDYGFRVFGLSVAEFK
                     QYEAQFLPILDQVIKNKLAFELNAKSAYLYDNLALYEYVIDLYLSRGGTLFSVGSDGH
                     YLEHFRFHFDDL"
BASE COUNT          131 a           69 c           57 g           96 t
ORIGIN      
        1 agatcatcga aatggaaacg gaaatgttca aggtaatgcc catctgatcc aacactaaac
       61 aaggtaccac cacgtgaaag atataaatcg atgacatatt catataacgc taaattgtca
      121 tacaagtaag cgctcttagc atttaattcg aatgccaatt tatttttgat tacttgatct
      181 agaatcggta aaaactgcgc ttcatattgt ttaaattcag caacggatag accaaatacc
      241 cgaaaaccat agtcaaaatg agcaaagaca tctgcttcta taacaactag tgcttctgaa
      301 agttgtgcaa aatactgagg aatcacaata gcaggatcca tatctttgac ttc
//
LOCUS       DAJSKS010000354          347 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJSKS010000354 DAJSKS010000000
VERSION     DAJSKS010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.366"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
BASE COUNT          143 a           34 c           58 g          112 t
ORIGIN      
        1 aataaataaa aatagggaat tttctattgc aattttataa agagattgct atattattat
       61 ttgttctaaa tgattccttt aaggtgtcac taaaaattat aaaaaatagt gttgacgcac
      121 ttttgatgtc atgttataat aacgaagtcg tttggatgag atagctaata aagcttgatt
      181 ttatcgaaaa aaacttcgaa aaagttattg acaaataaca ataaagatgg tatgataatc
      241 aagtcgctgc taatgagtgg caaagacgtt tagaaaaaat aaacaaagaa gttgttgaca
      301 ttctcttctg aatctgataa gattaaagag ttgttaaaac aaaaaga
//
LOCUS       DAJSKS010000355          343 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJSKS010000355 DAJSKS010000000
VERSION     DAJSKS010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.367"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..>343
                     /locus_tag="ON396_002964"
     CDS             <1..>343
                     /locus_tag="ON396_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR3114975.1"
                     /translation="WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQ
                     SVGLYTKTEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNR
                     RNGTLFGFSVST"
BASE COUNT          115 a           72 c           70 g           86 t
ORIGIN      
        1 ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac gactccataa
       61 acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg gctccgcctt
      121 tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg tgaagtatct
      181 taacaattta atagaacaag accatcgacc tattaaacga cgaaataaat tttatcaaag
      241 tctccgtaca gcctcttcca cgattaaggg catggagacc attcgaggaa tatataaaaa
      301 gaaccgaaga aatggaacgc tcttcggctt ttcggtgtct act
//
LOCUS       DAJSKS010000356          340 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJSKS010000356 DAJSKS010000000
VERSION     DAJSKS010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.368"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            62..280
                     /locus_tag="ON396_002965"
                     /pseudo
     CDS             62..280
                     /locus_tag="ON396_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          115 a           51 c           61 g          113 t
ORIGIN      
        1 tattttttaa cgtaattagg cagaagtcac tcatcctcta taggtttgtg atgaaattgc
       61 cattgatcca tttaagtaag agcaaaaaac tggaggttta tcaggtaatt ggtctaatgg
      121 atttaagtat gcggtcaccg tgtatagagt tgcttatgag attcaagaaa ataaagttat
      181 tccagtattg ctatatggaa cacataacaa tttttatgaa ttagccgaga acactcgcaa
      241 ctttattcgt gtagatgaat cggcttcggt tgagttataa ctttagctat atcaatcaac
      301 cgaccgacat atgttaaatc tatatataat aaaaatacct
//
LOCUS       DAJSKS010000357          335 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJSKS010000357 DAJSKS010000000
VERSION     DAJSKS010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.denovo.369"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..>335
                     /locus_tag="ON396_002966"
     CDS             <1..>335
                     /locus_tag="ON396_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HCR3114976.1"
                     /translation="YVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGER"
BASE COUNT          125 a           53 c           77 g           80 t
ORIGIN      
        1 ttatgttcgc cgatttagag aaacgacggt gtttgaggat atttttgcct atattttaga
       61 acaagcagtt aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat
      121 caaagctaac gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc
      181 ctatcaggat gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc
      241 ttttactttg gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga
      301 tccggaaagc ggctactacg tcaaaggcga acgag
//
LOCUS       DAJSKS010000358        24952 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.guided.026, whole genome shotgun sequence.
ACCESSION   DAJSKS010000358 DAJSKS010000000
VERSION     DAJSKS010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.guided.026"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(<1..276)
                     /locus_tag="ON396_002967"
     CDS             complement(<1..276)
                     /locus_tag="ON396_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114977.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACG"
     gene            complement(539..3355)
                     /gene="uvrA"
                     /locus_tag="ON396_002968"
     CDS             complement(539..3355)
                     /gene="uvrA"
                     /locus_tag="ON396_002968"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR3114978.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(3365..5359)
                     /gene="uvrB"
                     /locus_tag="ON396_002969"
     CDS             complement(3365..5359)
                     /gene="uvrB"
                     /locus_tag="ON396_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCR3114979.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            5618..7120
                     /locus_tag="ON396_002970"
     CDS             5618..7120
                     /locus_tag="ON396_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HCR3114980.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            7122..7859
                     /locus_tag="ON396_002971"
     CDS             7122..7859
                     /locus_tag="ON396_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR3114981.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(8030..9223)
                     /locus_tag="ON396_002972"
     CDS             complement(8030..9223)
                     /locus_tag="ON396_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR3114982.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(9302..9508)
                     /locus_tag="ON396_002973"
     CDS             complement(9302..9508)
                     /locus_tag="ON396_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR3114983.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(9501..10643)
                     /locus_tag="ON396_002974"
     CDS             complement(9501..10643)
                     /locus_tag="ON396_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR3114984.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            11155..11790
                     /locus_tag="ON396_002975"
     CDS             11155..11790
                     /locus_tag="ON396_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3114985.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            11944..12021
                     /locus_tag="ON396_002976"
                     /pseudo
     CDS             11944..12021
                     /locus_tag="ON396_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            12266..12382
                     /locus_tag="ON396_002977"
     CDS             12266..12382
                     /locus_tag="ON396_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCR3114986.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            12398..14317
                     /gene="tet(M)"
                     /locus_tag="ON396_002978"
     CDS             12398..14317
                     /gene="tet(M)"
                     /locus_tag="ON396_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR3114987.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            14436..14603
                     /locus_tag="ON396_002979"
     CDS             14436..14603
                     /locus_tag="ON396_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR3114988.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(14663..15016)
                     /locus_tag="ON396_002980"
     CDS             complement(14663..15016)
                     /locus_tag="ON396_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114989.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            15520..15939
                     /locus_tag="ON396_002981"
     CDS             15520..15939
                     /locus_tag="ON396_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR3114990.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            15965..16171
                     /locus_tag="ON396_002982"
     CDS             15965..16171
                     /locus_tag="ON396_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114991.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(16399..16572)
                     /locus_tag="ON396_002983"
     CDS             complement(16399..16572)
                     /locus_tag="ON396_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3114992.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            16548..17213
                     /locus_tag="ON396_002984"
     CDS             16548..17213
                     /locus_tag="ON396_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR3114993.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(17295..18461)
                     /gene="dnaJ"
                     /locus_tag="ON396_002985"
     CDS             complement(17295..18461)
                     /gene="dnaJ"
                     /locus_tag="ON396_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR3114994.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(18612..20441)
                     /gene="dnaK"
                     /locus_tag="ON396_002986"
     CDS             complement(18612..20441)
                     /gene="dnaK"
                     /locus_tag="ON396_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR3114995.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(20492..21055)
                     /gene="grpE"
                     /locus_tag="ON396_002987"
     CDS             complement(20492..21055)
                     /gene="grpE"
                     /locus_tag="ON396_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR3114996.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(21149..22192)
                     /gene="hrcA"
                     /locus_tag="ON396_002988"
     CDS             complement(21149..22192)
                     /gene="hrcA"
                     /locus_tag="ON396_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HCR3114997.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(22426..23591)
                     /locus_tag="ON396_002989"
                     /pseudo
     CDS             complement(22426..23591)
                     /locus_tag="ON396_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
     gene            complement(23607..23834)
                     /locus_tag="ON396_002990"
     CDS             complement(23607..23834)
                     /locus_tag="ON396_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR3114998.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            24318..24908
                     /locus_tag="ON396_002991"
     CDS             24318..24908
                     /locus_tag="ON396_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HCR3114999.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
BASE COUNT         7228 a         5033 c         4548 g         8143 t
ORIGIN      
        1 accacaagca atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg
       61 aagcatcgtc tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac
      121 aattttactg tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc
      181 gtatgcttca ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat
      241 agggataaat tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc
      301 ttgtttttct tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag
      361 gaaagtcctc cgattgctca attctttttt gaacggattg aacttatagg aagtttttat
      421 ctataccaat cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata
      481 aaacaagcaa aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt
      541 ataccattat ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt
      601 ctggtgttcc agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa
      661 tgatataatc tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac
      721 cagcatctac taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc
      781 ctgtcgttgg ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac
      841 tggcaagttt catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg
      901 tcacataacc taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt
      961 gcttgaagaa ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc
     1021 ctttgtaatg cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag
     1081 gaacatagac atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag
     1141 cttcacaacg accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt
     1201 ttgcctcgtt cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg
     1261 ctggattact tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga
     1321 tactttcgta gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt
     1381 tttgcgctaa cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca
     1441 cacctgtgac tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt
     1501 tttctgaagc accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta
     1561 cagggattga ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta
     1621 cttcttcagg agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga
     1681 cgtcaatcaa ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg
     1741 tgttgccaag atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat
     1801 gcaatccgat cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt
     1861 gtgtagccaa tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca
     1921 aagttagata gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta
     1981 aaatcggttt ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta
     2041 ctgctttttg aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag
     2101 atagagcttg gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat
     2161 aaagacgcat ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat
     2221 tcttcaatac cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat
     2281 aatggaaatg gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta
     2341 attcttcgaa tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt
     2401 gaggataata ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct
     2461 tcgttctatc tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac
     2521 agtctggaca agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa
     2581 cagtaaatcc gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga
     2641 tcacgtcaac cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata
     2701 ggcgagaacg aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt
     2761 ttttgttttt ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa
     2821 cgcggacgta tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct
     2881 ttaccacgat cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat
     2941 ctgccatttg atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat
     3001 gtcctacacg tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg
     3061 tcgaccgagg atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt
     3121 cgatgctatc tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa
     3181 ggctctctac gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt
     3241 ttccagaacc ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta
     3301 tatttttcaa gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt
     3361 cctcctactt tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa
     3421 aatccaaagc ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt
     3481 tttcttcctt cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg
     3541 attctttcgt aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc
     3601 catgttcttc attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta
     3661 gacgcataga atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag
     3721 cagctcgtcc aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg
     3781 catccaagat agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga
     3841 ttccaactaa cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca
     3901 gcgttttgat atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg
     3961 tgaggtcttc tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga
     4021 ctcgttcatt tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga
     4081 tcactgggtc aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt
     4141 catgttcata tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa
     4201 actcttctaa ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta
     4261 ctagcatctg ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta
     4321 cgtgcgattc gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag
     4381 gcggttcacc ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt
     4441 agcccatttc taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt
     4501 ccagaagttt gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat
     4561 tcgctacggc atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg
     4621 ctacgtgttc cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt
     4681 cgacctcatc tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa
     4741 tctccacgac atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt
     4801 cgaattggat actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc
     4861 gaatcgaaac tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa
     4921 cggatgcaat gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta
     4981 atttgtcgat ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca
     5041 cataagcttc aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag
     5101 ggaaaaattc cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa
     5161 tcagtgttgg tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc
     5221 cagtcgcacc taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt
     5281 taatcgcttc aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg
     5341 aggtatctct ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac
     5401 caataaaaag aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca
     5461 tactttcgcc acttttttat cttattatac atgttatgtt ttataacata aaatgttaga
     5521 aactcgcatt tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc
     5581 tataataata aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca
     5641 ctcatttgtt ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc
     5701 tgacaacact acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa
     5761 aaaagacgta tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc
     5821 aaagggcgag tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg
     5881 atttaacatc cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg
     5941 acaagcagac ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga
     6001 tttttctgat tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc
     6061 gatcaagtct tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag
     6121 tgctgaattt ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc
     6181 tgctgatcaa ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta
     6241 tccggtaatc ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga
     6301 atctggcgga aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat
     6361 gttcaatgaa ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga
     6421 atatgtagcg gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt
     6481 tcttggactt atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct
     6541 cctaactttg atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag
     6601 tgtagcacca atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg
     6661 aattccaatg atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt
     6721 tacgattcct gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat
     6781 cgcagagatt gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag
     6841 aagtttaggt ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg
     6901 catcatgatc ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat
     6961 ttccgcaatc ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac
     7021 acaaagtacg tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt
     7081 gacaaaatta gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag
     7141 ttgagcattt agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca
     7201 tcaacgaagg agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct
     7261 tgcgttgctt gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc
     7321 acttgatgga taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc
     7381 aacactttaa cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg
     7441 acctgaagaa agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag
     7501 ttggtctaga agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc
     7561 gtgtggctat tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta
     7621 cttctgcact cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag
     7681 aacaaggtat gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca
     7741 atcgcgtcat gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct
     7801 ttgaaaatcc gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa
     7861 ataaattata tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct
     7921 tattttatag gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat
     7981 cttcttatat tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta
     8041 gacgctgaac ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga
     8101 tactgatatt tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg
     8161 ccagtcttgt gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt
     8221 ccttgtggtg cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg
     8281 caactttggg cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa
     8341 atggttctgc ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc
     8401 ttaatggtac ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt
     8461 ccttgctctt tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg
     8521 ctactgtcag tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat
     8581 catcataatg cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct
     8641 gttcctctgt caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga
     8701 aatcaaaggg atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta
     8761 acgagcgttt atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg
     8821 cccattcctt agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct
     8881 ctttcaataa ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc
     8941 tctgtgcgtt ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga
     9001 taccgtcctc aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc
     9061 gttttccctt tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt
     9121 ttccaaatgc gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag
     9181 tatgcaggat acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc
     9241 ttgttggaaa gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc
     9301 attagatagc gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc
     9361 ggttgccatt cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact
     9421 tgttttctcc gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc
     9481 atacgggaat atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct
     9541 atgtccgtac acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca
     9601 tcaatcattt tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt
     9661 gacggcatga cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg
     9721 ttcatttcaa aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga
     9781 aattgcacaa aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac
     9841 aattcctcaa ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata
     9901 atatccgtat gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat
     9961 tttgtgcttg cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt
    10021 agttttccac cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg
    10081 tatttttcag acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca
    10141 aaacgtcgga taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg
    10201 ttctgtgcct gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat
    10261 acgcccatgt gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct
    10321 ttatagccat aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc
    10381 tttcggataa tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc
    10441 agataaaaag gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt
    10501 tccttgctgg cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg
    10561 gtaagacttg caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca
    10621 agtgcaaatt ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct
    10681 gtaaacctct attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact
    10741 cacaactttg ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta
    10801 gataacgggg gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct
    10861 tgacacaagg tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt
    10921 tgtcagcaag ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct
    10981 acggatttaa tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct
    11041 tattcgatta caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc
    11101 tttacaaatc atattaagca tgatagactt ataatagcga agatataacg agatatggag
    11161 ggttatgcaa tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg
    11221 ggaatgaaac aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag
    11281 attgaaagag ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg
    11341 gacgttacgc ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag
    11401 gaagtctgtc agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac
    11461 tatgataact tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg
    11521 gaatatatta cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc
    11581 aaagaattta tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac
    11641 agtatggata tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc
    11701 gtgaaaatga taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg
    11761 aataacaatt ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa
    11821 acaaaaagcc cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc
    11881 tttttctttt ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta
    11941 aatcaggcaa aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt
    12001 aattggaaga ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg
    12061 tactctcttt gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt
    12121 taagtatcta tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc
    12181 aatttgatag tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta
    12241 cccagtttaa gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct
    12301 ttgtatgcct atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt
    12361 ttatgccctt ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt
    12421 tttagctcat gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg
    12481 agcgattaca gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt
    12541 agaacgtcag agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac
    12601 gaaggtgaac atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc
    12661 attatcagtt ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca
    12721 aactcgtata ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa
    12781 taagattgac caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact
    12841 ttctgccgaa attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa
    12901 ctttaccgaa tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa
    12961 atatatgtcc ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt
    13021 tcagaattgt tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga
    13081 taaccttata gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga
    13141 actttgcgga aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat
    13201 acgcctttat agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa
    13261 aataaaagtt acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc
    13321 ttattctgga gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg
    13381 agatacaaaa ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac
    13441 aactgttgaa ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat
    13501 ctcagatagt gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact
    13561 ttctttctta gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca
    13621 tgtggagata gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc
    13681 agaatatacc attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc
    13741 tgtatcaccg cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata
    13801 cttaaatcaa tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg
    13861 attgtatggt tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag
    13921 ccctgttagt accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt
    13981 aaaaaaagct ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca
    14041 ggaatatctt tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac
    14101 tcaattgaaa aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga
    14161 atatcgtagt gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa
    14221 agggtaccat gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat
    14281 agataaagta cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa
    14341 tatggtttct tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa
    14401 aggaggagat agttattata aactacaagt ggatattgtg tcctgtatgt ggaaataaaa
    14461 cacgattaaa gataagggaa gatactgaat taaaaaaatt ccccctctat tgtccgaaat
    14521 gcagacaaga aaatttaatt gaaataaagc agttcaaagt aactgtgatt acagagccag
    14581 acgcaaagac gcagagccga taaaatgaga ttaatacaat ctcattttat cggctctttc
    14641 cgttatgtat ggattctttt aattagtctt cgatgtttct tgcttcgttg ataccgctgg
    14701 ctaaagattc cattaaggat agttctttgt ctgtaaagct atccatgtat ttctctatct
    14761 gtaatcgtcg ggtgcttttt accaagttat tagcaggtaa gaaaaattca tcaacggaaa
    14821 catgaagtaa cgatacaagg tcataaagaa cttgtatgct ggggtgttgc cctttatttt
    14881 caatattagt taagtaccgt gggtcaattt caatcaatgc tcccacttgt tcacgagtta
    14941 aacctcgttt caatcgagct tctttaatgg ctaaaccaaa ggctctaaaa tcatatttat
    15001 cttctttttt acgcatagta gaccacctct atacatttta ttgttcctac tgaattaaaa
    15061 acaggtatag aaaaacgtgt tatatggttt ataggtttat atttaataaa aagcactact
    15121 aaacgccaat aaaaaaaacc gttatatggt agtgctattt acgctgttaa aatattgtat
    15181 attacttcca aatggcggtt tgttggaggt caacgtcgcc atgaagtaca tcatatacaa
    15241 taaatttcct tacattgggt tcttgtcaaa aaaagtcgtc tatctgcaat agataagtac
    15301 gtccaccaat gtggttttat aaatcatata gatagaataa cagaagcatg taaacagaga
    15361 aataaatctg tttatatgct tttttggcta ttcagaactt ttttacaaag tttatttatc
    15421 agtaatgcaa caaatccccc tttcacattg ggactaagag tgaaaggaga taaacgagca
    15481 aggctcactt cctttcctag acagaaaggg ggtgagatta tgaggacatc ttctttcgag
    15541 catatcgtca gattacagtt caattcttta atgctgattg tcattaagaa caaagtgaaa
    15601 agtcgaaaca gacagcttgc aagacattct aaacgtgaag tattgttttg tgaaatagcc
    15661 gaaacgaaac aagttgaacg tggaacgatt gatacctgtt tttatgattg tgtatcattt
    15721 aaagtcttgc actttacaat ttatgtatct gatgaaacac ttggtacagt tttacataga
    15781 ctatcagaaa aacagcgtag tgcgatttta ttacgttatt ttcaaggaat gaacgacaga
    15841 gaaatttcag aactgtacca tgtatcacgt tctgctattt gcagtagaag aactagagga
    15901 ctaaggaagt taaaaatgct tttgagcgaa aggaagtaaa gattatggct atccctcttt
    15961 tgaattgatt tgcaaagcgt caagcggaga tgaaatggca gttaaagaga ttttgaagtt
    16021 ttatgacggt tatatttcta aattatgctt gcgtcctttt taccactctg aaagtggtaa
    16081 aatcattatg caggttgatg aagaactaaa aggagaaatt catacagaca tgatgaaagc
    16141 aatattgaaa tttgaaatcg gagtgaagta atcaactgta taaaaaaatc gggagataca
    16201 cagtttcgtg ttatctccct tagtagtttt acggtcatat caaggctcat tcaatcgtgg
    16261 taaattcgtg gtaagatgag tgtttttcta tacttcaaaa tgcttgaatg tatagtgttt
    16321 atgcggataa ttgaaaatta gatataaaat ttaataaaaa ccaaaaatgc acggtcgcca
    16381 tgaagggacc gtgtattttt atcttcttat tttctcacct aatcgtatca gccgcatcgc
    16441 ttgttttcct gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat
    16501 cggttcattg atgatatcaa gtacatagtc gataccaccc tcacttaatt gagcttgatt
    16561 tttcttctcc atattatgta gttgtgggac aactttctga caatcagcaa ttgtgtcaag
    16621 aacggatcaa tcctttattg gagttgctcg aaagggtctt ctcttattac ggacaggctt
    16681 tgctcactgt acatagacaa caaatcatta tcctagtcaa tcggatttcc agagcctcat
    16741 tgctatcatt gcttgataaa atccagacaa agtttaataa aatgtatcag ttacagctaa
    16801 actttggaat tgggtcgctt tgttatacag agcaagagac accgcaatct ttccttcatg
    16861 ccaaacaagt ttgcgaatgg atagctttcc atcaatctgt gaacgagatc cgcttttttg
    16921 aagatttaga cctcgggatc gttttaccag caatcccgtc agatcaacga acattatatg
    16981 taaaacgtat tttgaaaagc ctgacagaag aagaagtaca tttgttcaag aagactttgg
    17041 catgcttcag taaaaataac ggaagcatca aaaattgctc agaagaatta tttattcata
    17101 aaaacacctt gcaatatcga ttgaacaagt tccattctct cactggttat acccctagaa
    17161 actatgacga ttatcatatt ctaaagctgg cttttttgtt ggtacaaaca tgacaactac
    17221 aaaaaaacca ggcgaacata tgattgttcg tctggttttt aattattaat gacttagcgt
    17281 ttttactaga aaaactactt ccgctttttc ccgccaaaag catctttaaa cttatcgaag
    17341 aaaccgtctt cttgctgttc ttcaactttg atgccgccag cttcagcgaa tgcacgtaat
    17401 gcttctcttt gttgttcatt tagatttttc ggtgtgatca gttttacttt gacatgttgg
    17461 tcgcctgttg caccaccacg taacttcgga gcgccttttc cacgtaaacg gaagttcgta
    17521 cctgtctgtg ttccagcagg gatcttcaat ttcacatctc catggacagt tggtacttgg
    17581 acttcatccc ccaaagctgc ttgtacaaaa cttaaaggca attcatagta aatttcagaa
    17641 ccttcacgat caaagatatc actttcttct acacggaaga ccacatacaa atcaccaaat
    17701 ggtccgccgt tttctccagc ttctccttga ccagctaaac gcatttgttg tccttcttct
    17761 acaccagctg gtacattcac tttaactgaa tgtgctttct tctcatgtcc tgagccatga
    17821 caggttggac aaggttcttt gatttctttc cctgtcccat gacaaacatc acaagtttga
    17881 cggctcatca ctcgtccaag tggtgtttgg cgttctacat taatcgtacc tgcaccatga
    17941 catttatgac aagtagttgg ttgtgtgcct ggttttgcac cgttcccacc acaagtatga
    18001 cagatttctt cccgattata cttgatttct ttttctacac caaaaatcgc ttcttcaaaa
    18061 gacaagtcga ccgtatattg caaatcagca ccttgacgag gcgcatttgg atcgactgac
    18121 cgaccgcctc ctccaaagaa agagtcgaag atatcttcaa aaccaccgaa gccgccacca
    18181 gagaagccgc cgaatccacc aaatccgcca ccagctccac cagcaccgta atttggatct
    18241 gtgcctgcat gcccgtattg gtcatatgca gcgcgttttt gcgggtcact taaaatctca
    18301 tatgcttctg atacttcttt gaatttttct tccgcatcag gttctttatt gatgtcagga
    18361 tggtattttt tcgaaagctt acgataagct ttctttattt catcttctga tgctcctttt
    18421 gagagcccta agacttcata ataatcacgt tttgttgcca taggctttcc tcctattggt
    18481 tctcatcatt ttcacgtcgc ttgttatcat atcatatttt gtttgaaaca aaaaccgata
    18541 cttcaaacta tttttcaaaa gaaaaagcca aagtagcaac tttggccttc tctcgaaatt
    18601 cagtgaactg attatttatc gtcgccatcg acttcttcaa aatcagcatc aacgacatca
    18661 tctgctccgc cttgagctgc ttctgggttt tcttgagctt gttgttgtgc cgcttgttcg
    18721 tatagtttaa cagtcaagtt ttgaacgatt tcgttcaatg catcgcgttt agttttcatt
    18781 tcttcgatgt tgtttgcttc aacagcggct ttcaattcat cgcgagctgt ttctgctttt
    18841 ttcacttctt cttcgtccac tttgccttct aattctttca atgttttgtc cacagagaat
    18901 agcaatgcat ctacatcatt acgtaggtca acttcctctt tacgagcttt atctgcttct
    18961 gcatttgctt ctgcatcttt taccatacgt tcgatttctt cgtctgttag acctgaagaa
    19021 gatttgatcg tgattttttg ttctttttgt gtacctagat ctttcgcaga tacatttaca
    19081 atcccgtttt tatcaatatc gaatgttact tcgatttgtg gaataccacg aggagctgct
    19141 ggaatatctg tcaattggaa tcttcctaga gttttgttgt cagcagccat tgggcgttca
    19201 ccttgtaata catggatgtc tactgctggt tgattatctg ccgcagttga gaatacttgt
    19261 gatttacttg ttggaatcgt tgtattgcgg tcaatcaatt tagtgaacac tccacccatt
    19321 gtttcgatac ctaatgacaa tggtgtaacg tcaagtaaaa caacatcttt cacatcacca
    19381 gtgatcacgc caccttggat agcagcaccc atagcaacta cttcatcagg gttaactgat
    19441 ttgtttggtt cttttcctgt ttctttacgt actgcttcta caactgcagg gatacgagta
    19501 gatccaccga ctaaaataac ttcgtcgatt tctgattgtg ataaacctgc atcttttagc
    19561 gcttgacgta ctggtacttt tgtacgttct actaagtcag ctgttaattc atcaaatttt
    19621 gcacgtgtca atgtcatttc caaatgcaat ggtccagatt ctcctgctgt gataaatggc
    19681 aagctgattt gtgtgcttga tacgccagat aaatcttttt tcgctttttc agcagcgtct
    19741 ttcaagcgtt gtaaagccat tttgtcttga gacaagtcaa tgctgttttc ttttttgaat
    19801 tctgctacca tgtagtcgat gattttgtta tcaaagtcgt ccccacctag atggttgtcg
    19861 ccagctgttg acaatacatc aaagacaccg tctcctagtt caaggataga tacgtcaaaa
    19921 gtaccgccac caaggtcgaa tactaagatt ttttcgtcgc gatctgtttt atctaaacca
    19981 taggcaagag cagctgcagt tggttcattg acgatacgtt ctacttctag accagcgatc
    20041 ttaccagcat cttttgttgc ttgacgttgt gcatcattaa agtaagctgg cacagtgata
    20101 actgcttttt cgactttttc acctaaatag tcttctgcga atcctttgat atattgaagg
    20161 atcattgctg agatttcttg tggtgtatat gatttacctt ctacatctac tttgtaacct
    20221 gcttcaccga tatggcgttt gattgatgaa atcgtatttg ggtttgtcac agcttgacgt
    20281 tttgcaactt cccctacttg gatttctcca tttttgaatg atacaacaga aggtgttgta
    20341 cggtttcctt ctgggttagc gatgattttt gcttcgtttc cttccaatac tgctactgca
    20401 gagttagtcg tacctaagtc aataccgata attttactca taattaacgt ctccttcttt
    20461 aattgaaagt gtaagtttta tatccgcaca attattgtgc aacaatgacc atggttggtc
    20521 ttaacacgcg atcatgtaat ttgtagcctt cttgtagtac ttctacaatc gtatcggctg
    20581 gtgtatcttc agtcgctgga acagtttgta ctgcttgatg aagattagga tcaaaggctt
    20641 ctcctttagc agggattttt tcaattcctt cttcttccag tgcgttacgc aaactttcca
    20701 agaccatttc cacgcctttt ttcaaacctg ccccttgatc atctgacact tctgtggcta
    20761 atgcacgttc caggttgtca attgatggaa gtaatttttt ggctaaatct tgtgaacggt
    20821 atttttgtaa ttgctcacgc tcattttttc ctcgatttgc catatttgcg atttcagcac
    20881 gagcccgcaa gtatttatct tccatttctt ctaactcagc ttttaatttc gctgtttcga
    20941 attcttccgc ttcagcttct gaaatacccg aatcttctgc agcttcgaca tcgatttccg
    21001 gttctggctg agcatctgtc atttctttat ctagttcttc ttgattttct ttcacgatac
    21061 agtcttcctt tcattattca gtcagctgtt tgattgctct tactaagaaa gagagaacga
    21121 tcagctgcaa atagcttcgt tattcacatt acgagtttga caagtcaagc gaacggtagt
    21181 aatcagcaag tttagctgct agttctcttc gataaacatc gactagaccg aacatccgag
    21241 aataaggcat actagtaggc cctaacaagg ctattgtacc tcttccatga ccatctatct
    21301 cgtaacttgc ttgaatcata ctcatattct gcaataaatc attgcctaat tcagcaccaa
    21361 tcttgatatg aatattgcta tccatcggag tgatcatttg tgtaagtccg tctgagtctt
    21421 tcataaaaga aaacatcgat ttgaactgat caagatcttg acggggctca taatccaaga
    21481 gattcatttg tccactgaca taaaccttat cttcaaaaac atgaccaaga acggtgtcaa
    21541 ataactgaga catcccttct gtcgtttgga aatatttatg aagaatcatc ggaatctccg
    21601 tacgaagttt atggtacacc gtcatcagag gatctccgac tagacggtcg tttacaatct
    21661 gtaccatttt ttctagttcc tgacttccca tatattcggg aagagtgaac acttggcttt
    21721 ctacattccc catatctgtt acgacgatag caataatctg tcgattattc aagggtacga
    21781 tacgaaagcc agtcagtcgg cggtctttta tttcaggtcc caatgaaaaa gtcgtatagc
    21841 ttgtcagctc agataatatc tcagcagatt gcttaataat ctcattgatt tcatgaaatt
    21901 ctctgcctag agactgacga atcagcagca aatcatcttc tgctacgcga gcaggtttta
    21961 acaaatgatc cacgtaataa cgatagccta aagcagatgg aatccgccca gaagaagagt
    22021 gagtcttcaa cagcaatcct tcatcttcta atgctttcat atcattgcga atcgttgctg
    22081 agctggcctt gactccctca gacatcaatg ttttggaacc aacgggcacg ccactactcg
    22141 tatagtgttg gatgatcaga cgtaaaatgt cgttttgtcg ctttgttaac atcttcctca
    22201 cctctcatta gcactcatat attctaagtg ctaatccact tataaatata gcaagaagcc
    22261 cttctcctgt caagagaaag actccatttt ttagcactct aaacacaaga gtgctaattc
    22321 taaataattg atgtaaatcc agttatatca acgtttcaat agtcctttat tgtatttttg
    22381 aacttttttt atttaaattc agataaaaaa caaatattct ttaaactatt tttcaaacaa
    22441 aaagcgctca aacacattgt tccccaaaaa ggtccctctt ggtgtcaaat aaacacggtc
    22501 ttcttcatta acaagtaacc cttcttgctc taattccctt agcacatcac cgtacaccga
    22561 ttcaattgtt ttttgatagc gatcatagaa atgtttttta gtaatccctg tttttttctt
    22621 agtcctaaga acatttcttc ttctatttga ttttttcgtg tcaatttttc ttcttccaat
    22681 accgggcgtt catttgctcg taatgggcgt aaatagtgtt ggattggtcc tttgttgcgg
    22741 taacgaatat ctccaagata accgctagca ccagctccaa acccaaaata gtggtcattg
    22801 ttccagtaca tgaggttatg ttgagattca tgtccggaga gcccaaagtt acttatttca
    22861 tattggtgac gtccatgctt ttccattgct tcaatcgtct cttcaaacat tctggtttcc
    22921 gtttcctgat caggcagttc gagtcttcct tgtcgtaccc agttcatgaa cattgttttg
    22981 ttttcgagga tcaatgagta caaagaatag tgaggcagat ccaaagccaa agctttttcc
    23041 aaagtatcca gatatccttc tagtgtttgt cccggtaatg cataaatcaa atcgatgctt
    23101 acattggtaa aattttctgc ttctaaaaat tgcatagttt gatacacatc ttctgcggtg
    23161 tgtttacgtc caattttttt caataagcga ttatcaaatg tctgcactcc catggataaa
    23221 cgattgacgc catagttttt cattacttga agtttttcac gggtcaaatc acctggattc
    23281 gcttctacag taaactcatt accattttga aaaggcagga tctctctagc acctgataac
    23341 agtcgatcaa gttgtttagc agataaagaa gttggtgtcc cgccccctac gtaaagtgtt
    23401 tctaaattcg ctgtcggatg actttctagc attattttca tttcattcag cagcatttcc
    23461 acatactcat ctactggctg cccttctaaa aaaactttat taaaatcaca gtaatagcag
    23521 atatgctcgc aaaatgggat atgaatgtac gcagaaacct tgttttctgc gggatttatt
    23581 gtttctatca ttgaaattct ttctccttac gtcgaaaagt cgtgcctgtt ttcgaccttc
    23641 ttaaaatcgc gttcaagcac caactcattg aaataagccg catatttaga atcaaggaaa
    23701 cctagctcta aaaatgcttg cttattgtca tcggagctac acggtgcttt tacagcctct
    23761 cgataaacgg taatcgcaaa gctgaccttc ggcattaagt caaaatctgc aaaaatatga
    23821 aaagctattt tcacagattc ctccttaagt gctcagacca caacgctttg cttacaacct
    23881 ctcatatacg catactaaac gtatttggcg aacaaatcca ctaaaaaatc caagaatcga
    23941 cttcttggat gattttttct ttcattaaac tggttaccta gttatatatc tctagggttg
    24001 ttttcatgtc tgagtgcccg atatgattta tttttcgttt ctataattac tttttgcctt
    24061 ccagtttctt ttaatattgt actcaagcaa gtgggaattg gttcttttgt catccatgta
    24121 tgcaaaatgt tataatgctg gtaaacaaaa atgattacct gtcattttta tttagttcgt
    24181 atttaattaa gttgactatc tgtatactct gtctaaaact taagaattgt tgttttcgct
    24241 gtcgctaccc aattttaatg aatgtctgta ggttaaaact gttgtccttg ttatagatag
    24301 taaaggagag cttgaaaatg aaaactgcga ttacgtttct attagaggaa tatgccgatt
    24361 gggaaggtgc ttatattact agcacactaa accaaaatga gaaatggcaa aacaaaactg
    24421 cttctatcca atcagaggtc acttccattg gaggattaaa aacaaaaatc gattacttaa
    24481 tcgaacaaat acctgaaaaa gttgaccttt ttattatgat cggtggcaat tcatgggaca
    24541 taaaaaatca tcatttatat caaataatcg ataaattgtt aaaaacagac gtaccagtag
    24601 ctgctatttg tggggcagtt gattatttag ctcagagcgg attattgcaa ggttacaaac
    24661 acaccggaaa tgcgcaattt ccttggttag aatcggaaaa ttatcagaat aaagaagatt
    24721 ttatagaaca acaagctgtg aaagatcgta atttgattac tgcgaatgga acagctccta
    24781 tagaattcac agacttgatt cttcaagcag tccattttga aacagaagaa ataagagaaa
    24841 aaacgattcg tctatacaaa cttggattct atgattacta taaaaagtac ggagatcctt
    24901 tctcctaatc attctcttca attaaatact ccttatcaat agtgtgacaa gt
//
LOCUS       DAJSKS010000359        13213 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.guided.072, whole genome shotgun sequence.
ACCESSION   DAJSKS010000359 DAJSKS010000000
VERSION     DAJSKS010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.guided.072"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(204..2162)
                     /locus_tag="ON396_002992"
     CDS             complement(204..2162)
                     /locus_tag="ON396_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR3115000.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="ON396_002993"
     CDS             complement(2159..3031)
                     /locus_tag="ON396_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR3115001.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="ON396_002994"
     CDS             complement(3373..4245)
                     /locus_tag="ON396_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR3115002.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="ON396_002995"
     CDS             4373..4942
                     /locus_tag="ON396_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCR3115003.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="ON396_002996"
     CDS             5009..5746
                     /locus_tag="ON396_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR3115004.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="ON396_002997"
     CDS             5977..7245
                     /locus_tag="ON396_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR3115005.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="ON396_002998"
     CDS             7732..8196
                     /locus_tag="ON396_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR3115006.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="ON396_002999"
     CDS             8180..8809
                     /locus_tag="ON396_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR3115007.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="ON396_003000"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="ON396_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCR3115008.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="ON396_003001"
     CDS             10688..11134
                     /locus_tag="ON396_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCR3115009.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="ON396_003002"
     CDS             complement(11333..11848)
                     /locus_tag="ON396_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR3115010.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="ON396_003003"
     CDS             11965..13167
                     /locus_tag="ON396_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR3115011.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DAJSKS010000360         8160 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.guided.121, whole genome shotgun sequence.
ACCESSION   DAJSKS010000360 DAJSKS010000000
VERSION     DAJSKS010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.guided.121"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(134..577)
                     /locus_tag="ON396_003004"
     CDS             complement(134..577)
                     /locus_tag="ON396_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCR3115012.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ON396_003005"
     CDS             complement(795..2135)
                     /locus_tag="ON396_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR3115013.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ON396_003006"
     CDS             2404..3300
                     /locus_tag="ON396_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCR3115014.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ON396_003007"
     CDS             complement(3450..4391)
                     /locus_tag="ON396_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR3115015.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ON396_003008"
     CDS             4517..5638
                     /locus_tag="ON396_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR3115016.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ON396_003009"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ON396_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCR3115017.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="ON396_003010"
     CDS             7426..7992
                     /locus_tag="ON396_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR3115018.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         2709 a         1514 c         1605 g         2332 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
//
LOCUS       DAJSKS010000361         7959 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.guided.126, whole genome shotgun sequence.
ACCESSION   DAJSKS010000361 DAJSKS010000000
VERSION     DAJSKS010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.guided.126"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            128..1564
                     /locus_tag="ON396_003011"
     CDS             128..1564
                     /locus_tag="ON396_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR3115019.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="ON396_003012"
     CDS             1753..4200
                     /locus_tag="ON396_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR3115020.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="ON396_003013"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="ON396_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCR3115021.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="ON396_003014"
     CDS             complement(5690..6793)
                     /locus_tag="ON396_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR3115022.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="ON396_003015"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="ON396_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCR3115023.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2665 a         1359 c         1729 g         2206 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DAJSKS010000362         5626 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.guided.153, whole genome shotgun sequence.
ACCESSION   DAJSKS010000362 DAJSKS010000000
VERSION     DAJSKS010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.guided.153"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            260..1171
                     /locus_tag="ON396_003016"
     CDS             260..1171
                     /locus_tag="ON396_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HCR3115024.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1324..1809
                     /gene="vanZ"
                     /locus_tag="ON396_003017"
     CDS             1324..1809
                     /gene="vanZ"
                     /locus_tag="ON396_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HCR3115025.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            2328..2612
                     /locus_tag="ON396_003018"
     CDS             2328..2612
                     /locus_tag="ON396_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3115026.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            2978..3277
                     /locus_tag="ON396_003019"
     CDS             2978..3277
                     /locus_tag="ON396_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3115027.1"
                     /translation="MNHDECKTYIKNSLLEIRKLANIYTLETFKKELEKRNIYLETKS
                     DKYFSSEGEDYIYKLIENNKIIYSISGKKLTYKGKKSFSKHAILKQLNEKANQVN"
     gene            3320..4810
                     /locus_tag="ON396_003020"
     CDS             3320..4810
                     /locus_tag="ON396_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HCR3115028.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            5158..5328
                     /locus_tag="ON396_003021"
     CDS             5158..5328
                     /locus_tag="ON396_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR3115029.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            5342..>5626
                     /locus_tag="ON396_003022"
     CDS             5342..>5626
                     /locus_tag="ON396_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR3115030.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMN"
BASE COUNT         2064 a          810 c         1065 g         1687 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt caaggaggta aggatggcgg aatgaaacca acgaaattaa
     1201 tgaacagcat tattgtacta gcacttttgg ggtaacgtta gctttttaat ttaaaaccca
     1261 cgttaactag gacattgcta tactaatgat acaacttaaa caaaagaatt agaggaaatt
     1321 atattgggaa aaatattatc tagaggattg ctagctttat atttagtgac actaatctgg
     1381 ttagtgttat tcaaattaca atacaatatt ttatcagtat ttaattatca tcaaagaagt
     1441 cttaacttga ctccatttac tgctactggg aatttcagag agatgataga taatgttata
     1501 atctttattc catttggctt gcttttgaat gtcaatttta aagaaatcgg atttttacct
     1561 aagtttgctt ttgtactggt tttaagtctt acttttgaaa taattcaatt tatcttcgct
     1621 attggagcga cagacataac agatgtaatt acaaatactg ttggaggctt tcttggactg
     1681 aaattatatg gtttaagcaa taagcatatg aatcaaaaaa aattagacag agttattatt
     1741 tttgtaggta tacttttgct cgtattattg ctcgtttacc gtacccattt aagaataaat
     1801 tacgtgtaag atgtctaaat caagcaatct gatctttcat acacataaag atattgaatg
     1861 aattggatta gatggaaaac gggatgtggg gaaactcgcc cgtaggtgtg aagtgagggg
     1921 aaaaccggtg ataaagtaaa aagcttacct aacactatag taacaaagaa agcccaatta
     1981 tcaattttag tgctgaggaa ttggtctctt taataaattt ccttaacgtt gtaaatccgc
     2041 attttcctga cggtaccccc actagcagcc atgctttttt tgaatctttg taggacgttt
     2101 taaagagaat tacccacttt aaaattaaac atgcgcttag aatcgcttta ggaaacacga
     2161 tccagtccaa taatcgtcga taaaaacttt tgaaaaaggt tggtgaaatt acctactttt
     2221 ggaataatca caaatcacaa gtgattaatc acttgtttat taagatatta aaagctataa
     2281 tttaaataaa gcgtgaattt tattacacaa aaagaggggg gagaaacttg gaactagcat
     2341 ttagagaaag cttaaaaaag atgagaggta ccaaatcaaa agaaaaattc tcccaagaat
     2401 tagaaatgag tagatcaaat tattcacgaa tagaatcagg aaaatcagat ccaaccataa
     2461 aaacactaga acaaattgca aagttaacta actcaacgct agtagtggat ttaatcccaa
     2521 atgagccaac agaaccagaa ccagaaacag aatcagaaca agtaacattg gatttagaaa
     2581 tggaagaaga aaaaagcaat gacttcgtgt gaataatgca cgaaatcgtt gcttattttt
     2641 tttaaaagcg gtatactaga tataacgaaa caacgaactg aatagaaacg aaaaaagagc
     2701 catgacacat ttataaaatg tttgacgaca ttttataaat gcatagcccg ataagattgc
     2761 caaaccaacg cttatcagtt agtcagatga actcttccct cgtaagaagt tatttaatta
     2821 actttgtttg aagacggtat ataaccgtac tatcattata tagggaaatc agagagtttt
     2881 caagtatcta agctactgaa tttaagaatt gttaagcaat caatcggaaa tcgtttgatt
     2941 gctttttttg tattcattta tagaaggtgg agtttgtatg aatcatgatg aatgtaaaac
     3001 ttatataaaa aatagtttat tggagataag aaaattagca aatatctata cactagaaac
     3061 gtttaagaaa gagttagaaa agagaaatat ctacttagaa acaaaatcag ataagtattt
     3121 ttcttcggag ggggaagatt atatatataa gttaatagaa aataacaaaa taatttattc
     3181 gattagtgga aaaaaattga cttataaagg aaaaaaatct ttttcaaaac atgcaatatt
     3241 gaaacagttg aatgaaaaag caaaccaagt taattaaaca acctatttta taggatttat
     3301 aggaaaggag aacagctgaa tgaatatccc ttttgttgta gaaactgtgc ttcatgacgg
     3361 cttgttaaag tacaaattta aaaatagtaa aattcgctca atcactacca agccaggtaa
     3421 aagcaaaggg gctatttttg cgtatcgctc aaaatcaagc atgattggcg gtcgtggtgt
     3481 tgttctgact tccgaggaag cgattcaaga aaatcaagat acatttacac attggacacc
     3541 caacgtttat cgttatggaa cgtatgcaga cgaaaaccgt tcatacacga aaggacattc
     3601 tgaaaacaat ttaagacaaa tcaatacctt ctttattgat tttgatattc acacggcaaa
     3661 agaaactatt tcagcaagcg atattttaac aaccgctatt gatttaggtt ttatgcctac
     3721 tatgattatc aaatctgata aaggttatca agcatatttt gttttagaaa cgccagtcta
     3781 tgtgacttca aaatcagaat ttaaatctgt caaagcagcc aaaataattt cgcaaaatat
     3841 ccgagaatat tttggaaagt ctttgccagt tgatctaacg tgtaatcatt ttggtattgc
     3901 tcgcatacca agaacggaca atgtagaatt ttttgatcct aattaccgtt attctttcaa
     3961 agaatggcaa gattggtctt tcaaacaaac agataataag ggctttactc gttcaagtct
     4021 aacggtttta agcggtacag aaggcaaaaa acaagtagat gaaccctggt ttaatctctt
     4081 attgcacgaa acgaaatttt caggagaaaa gggtttaata gggcgtaata acgtcatgtt
     4141 taccctctct ttagcctact ttagttcagg ctattcaatc gaaacgtgcg aatataatat
     4201 gtttgagttt aataatcgat tagatcaacc cttagaagaa aaagaagtaa tcaaaattgt
     4261 tagaagtgcc tattcagaaa actatcaagg ggctaatagg gaatacatta ccattctttg
     4321 caaagcttgg gtatcaagtg atttaaccag taaagattta tttgtccgtc aagggtggtt
     4381 taaattcaag aaaaaaagaa gcgaacgtca acgtgttcat ttgtcagaat ggaaagaaga
     4441 tttaatggct tatattagcg aaaaaagcga tgtatacaag ccttatttag tgacgaccaa
     4501 aaaagagatt agagaagtgc taggcattcc tgaacggaca ttagataaat tgctgaaggt
     4561 actgaaggcg aatcaggaaa ttttctttaa gattaaacca ggaagaaatg gtggcattca
     4621 acttgctagt gttaaatcat tgttgctatc gatcattaaa gtaaaaaaag aagaaaaaga
     4681 aagctatata aaggcgctga caaattcttt tgacttagag catacattca ttcaagagac
     4741 tttaaacaag ctagcagaac gccctaaaac ggacacacaa ctcgatttgt ttagctatga
     4801 tacaggctga aaataaaacc cgcactatgc cattacattt atatctatga tacgtgtttg
     4861 ttttttcttt gctgtttagc gaatgattag cagaaatata cagagtaaga ttttaattaa
     4921 ttattagggg gagaaggaga gagtagcccg aaaactttta gttggcttgg actgaacgaa
     4981 gtgagggaaa ggctactaaa acgtcgaggg gcagtgagag cgaagcgaac acttgatttt
     5041 ttaattttct atcttttata ggtcattaga gtatacctat ttgtcctaat atgattttag
     5101 cagtataatt gacttggtga ataggtcatt taagttgggc ataataggag gagtaaaatg
     5161 aaaaaattta tttatcgagt tttagaaaat gacgaagtgg tggctatttt taatgagcaa
     5221 caatatgcgc aagattttat cgcttacgaa aagacaattt ctgataagca atttgaaatt
     5281 gaaaaagtag atattgctga ttggttattg caaccgagag aattttagag gttggttgaa
     5341 aatggctaaa attggttatg cacgtgtcag tagcaaagaa cagaacttag atcggcaatt
     5401 acaagcgtta cagggcgttt ctaaggtctt ttcagacaaa ttaagcggtc aatcggtcga
     5461 acgcccacaa ttacaagcta tgcttaacta tattcgtgaa ggggatattg ttattgttac
     5521 tgaattagat cgattaggac gaaataataa agaattaaca gaattgatga atcaaattca
     5581 aattaagggg gcaaccctgg aagtcttaaa tttaccctca atgaat
//
LOCUS       DAJSKS010000363         5313 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.guided.159, whole genome shotgun sequence.
ACCESSION   DAJSKS010000363 DAJSKS010000000
VERSION     DAJSKS010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.guided.159"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            272..1486
                     /locus_tag="ON396_003023"
     CDS             272..1486
                     /locus_tag="ON396_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCR3115031.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1517..1735
                     /locus_tag="ON396_003024"
                     /pseudo
     CDS             1517..1735
                     /locus_tag="ON396_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2154..2627
                     /gene="dfrF"
                     /locus_tag="ON396_003025"
     CDS             2154..2627
                     /gene="dfrF"
                     /locus_tag="ON396_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HCR3115032.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            2754..3410
                     /locus_tag="ON396_003026"
     CDS             2754..3410
                     /locus_tag="ON396_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCR3115033.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            3592..4122
                     /locus_tag="ON396_003027"
     CDS             3592..4122
                     /locus_tag="ON396_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR3115034.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            4668..4910
                     /locus_tag="ON396_003028"
     CDS             4668..4910
                     /locus_tag="ON396_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HCR3115035.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            4907..>5313
                     /locus_tag="ON396_003029"
     CDS             4907..>5313
                     /locus_tag="ON396_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCR3115036.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIGSLSNDVGKEKS"
BASE COUNT         1807 a          747 c         1107 g         1652 t
ORIGIN      
        1 tcttttttat atattttata tgaagtattc tttattcctt atgaaaaaat atctttaaaa
       61 ataaaaaaag acgaaaataa acggttttct ataaaaagaa gaccgtttat tttttgtctt
      121 tatttgtgtg aaaaacgaaa aagagcacaa cacatcaagc ttttattaat tgtctgtctt
      181 ttataaatgc aagtattaat tcaaaaaaac agaagaaaaa aacaactaca ccttctttta
      241 tactcgtaat cgtagaaagg aggcgaaaaa aatgaataca ttacctatca atacaggaga
      301 tacagcattc atgattttat gtacagcaat ggtttgcctg atgactcctg gtctagcttt
      361 tttttacggc ggattagcac ggaagaataa tatactaacg atcatgtctc aatcttttct
      421 ctccgttggg ataacaacga ttatgtgggt atttggtgga ttcggattgg cctttggacg
      481 ggatatcggc ggtgttattg gaaacccggc agatttcttt ttgatgagac atgttacaag
      541 cttccccaac atgtttcatg gtgcaacgat tccattttta atgttctttc tttaccaact
      601 gatgtttatc gtgattaccg ttcctttgat gacaggagct tttgcaggga gattgaattt
      661 aaaaggctat attatacttg ttattttttg gaatctgctg atttatttcc ctgtttgcca
      721 ctggatatgg ggtggcggat ttttggatca gatgggattt agagatttcg ctggtggagc
      781 agtcatccac acgacagctg gattcggctc tttagcatgt atcctcacac ttggtcaaag
      841 aaagattcct ttaggagaaa gaagtcagca tagcagtctt atggcagctg ctatcggtac
      901 aggactgctt tggtttggct ggttcggttt taattccggt ggtgcacttc gagcagatga
      961 acaagcagtc aatgcatttg gttcaacatt catcgctctt gcatttgcga tgattacttg
     1021 gctgatcatt gcaaaagtaa aaggaaatgg ctttgatttt gtcgacgtac taacaggatc
     1081 cgttgctgga ttggcagcca ttacgccttg tgccggttat gtagaagcaa aaagtgccat
     1141 gctgattggg atcattgcgg gtatcgtctg tcatgctgct gttgattttc gtaaaaagaa
     1201 acaatgggat gatgcacttg atgtgtgggg tgttcatggc atgggcggat tcacaggtac
     1261 gattctgata gggatctttg catctaacag ccatttacta acgaatccaa gcagctggta
     1321 cttcttaggg attcaagtga ttggtgtcat cattactgcc gtgtatgcct atgtgctgac
     1381 aacagtgatt ttaaaatcgg cgatgcactt tacaacgatc actactacaa aagaagaaca
     1441 agaagaagga ttggactttt tcttctttgg caacaaactc aattgaaaca aattcaaata
     1501 gaaaagagga agattcatgc aacaaagcaa aacgaaaatt cctgcaaaaa aattaacgtt
     1561 ctttggcttt ttggctatga ccatctcaat ggtcgtttca ttatatgaat atcccacatt
     1621 cgcgacctca ggcttcagct tagtattttt cttgttatta ggcggtttgc tatggtttat
     1681 ccctgtggca ttgtgcgcag cagagatggc aacggtgaaa ggatgggaaa aaggtgtttc
     1741 ccaaacaaaa agatattaag aaacagccca tgtttacctg cttgcaaaca aaagaatatt
     1801 gctactagac taagagatac tacataagtg tgttggtatc tcttttttgt tgccgtgaaa
     1861 cagcagacta tttcatttct gcttgattac cttaaaactt attagtctat ggtttgtcaa
     1921 gggcttgttg cgtgagaaat gctgaatatc accctttaca aaacataggc tgataagtaa
     1981 cttttcaaaa gcagaaaaga aatggcaatg cttataacaa tatgatataa tgcagatgaa
     2041 gacaaaaaag gaatttgtcg agctggttag ttcgagataa ttttgagttt gttaagatgt
     2101 ggtgcacttg atggaatcac aggtacgaca tcatattatg gaggatgaaa tagatgatag
     2161 gtttgattgt tgcgaggtca aagaataatg ttataggcaa gaatggtaat ataccatgga
     2221 aaataaaggg agaacaaaag caatttagag agttaacaac gggtaatgtg gttattatgg
     2281 ggcgaaagtc ttatgaagaa atcggtcatc cgttgcctaa tagaatgaat attgttgttt
     2341 ccaccacaac agagtatcaa ggagataatt tagtttcagt taaatcatta gaagatgcat
     2401 tattattggc taaaggacga gatgtataca tatctggtgg atatggacta tttaaggaag
     2461 ctttgcaaat agtagataaa atgtatatca cagaagtaga tttaaatatt gaagatggag
     2521 atacattctt tccagaattt gatatcaatg attttgaagt tttgataggg gaaacacttg
     2581 gtgaggaagt gaaatatacg agaacatttt atgtaaggaa aaaatgaatt gagtaaattt
     2641 tggatttagg gtgttttcat aaatatattg ctttttatgc atatataaat tgttgtaaga
     2701 caaattccag tttgtatggg gcgataaaat cagaatttga gagaggattg aatatgaata
     2761 aggaatggtc tgaacttaat aaaacaatgc aggcacaaat aaaaaagaaa gatacttata
     2821 agaggggtat tgatacttta cttactttac gaagtcagtt aatacaaacc ttagtatctt
     2881 tcaaagaaga attatgcaga gaagatttta actcaatccc cttcataaac gctgatggtt
     2941 atcatagtaa gacgattgct tattctattt ggcatatttt ccgcattgaa gatatagttg
     3001 tgcatacagt gataaatgaa gatgaacaag tattttttgc aggcaattat caagagcgta
     3061 tcaattcacc tatcattaca acaggaaatg aactcatgaa acagcagatt gcagactttt
     3121 caaaacagct taatctggaa gaattatatt tatatatttt cgaagtatgg gaaagcactg
     3181 agaaaatgct tgaacggtta tcttatgatg aattgaaaag aaaaataccg aaagaaagaa
     3241 aaggatactt agaatcgttg aatgtagtaa acgacaatga aaaagcaatt tggctgattg
     3301 attattggtg taataaagat atttgtggac taattcaaat gccgttttca agacattgga
     3361 ttatgcacac agaagcctgt ttgcgtataa agaacaagat acattcatag gcaaaaattt
     3421 cagtttgtag aactaaggaa attggaattt aaggaggaat aaagtatggt tagtgatttt
     3481 ataagagcag catttccatg gattttaatg ggtttatttg tagcaatcag ttgttccctt
     3541 atgagtaaaa aagagaaata aattcctgtt tgtatttata aaagagaaaa aatgagaaaa
     3601 gtagttttat ttattgccat gagccttgac ggatatattg cggacggtaa tggtggagta
     3661 gcttggctaa atggtcatgg aaatgacaat gaaaacatcg acacttatac agaatttacc
     3721 aaagatatag atactgtctt aatggggtgg aatacttctc atcaggttgt tactgaactt
     3781 tctccgcaag aatgggtata taataaattt acaacttatg tattaacaca taaagagtgc
     3841 aattcttccc aacaaattca ttttacaagt gaaaatcctg ttttattatt ggaacggcta
     3901 aaacaagaag caggaaagga tatttggatt tgtggcggag caaatcttgt tcagcagttg
     3961 gtaagcaaaa atatgattga taaatattac atttctgtta ttcctactct gttaggaaat
     4021 ggggttcgtc ttttgggcaa catagacagg gaaataaagt taaggctttg taaaacacag
     4081 acttataacg gaataaccga tttgatttat atgcgtagat aactagagag aaacagaaaa
     4141 gctgttaaac cgacggagcg atccgaccac gttggcggga aaatccttaa gatttttccg
     4201 ccatgcttgc aagggggcgg ttcgcttcgg cggttcggcg agcttgcgag ccgttcaaag
     4261 cccccgttat ctaacaaggg gggcacaaaa aacaagaaac actatacact aacaagcgga
     4321 aaacgctgta tttataaggc aaaaccccat ttttacagta gtgatataaa ttgtacctat
     4381 atcactatga aaattgaata gtaaatatga gaaagacagt tgatttcaaa aaataagaaa
     4441 ttgattgtcg ttttcttttg cagaaattta agtgcaggca attatggtag ttgtcgtaat
     4501 tgctgtcagc tcagtgttta tcactgatgg tgtgaaagat ttacggttgg agatgttcaa
     4561 taagattatt ggtgcttaat tatttttatt gtagtgctga tatgagaatg ttataattgg
     4621 ggtataaaac atacaaataa atttgctgaa aaatgaggta tcaaaatatg gaaaatgaat
     4681 tactggaaaa cttaggaaga cttcacacaa ctgaacttgg cgttattaga attaaaaaga
     4741 acttatcttt gaatgtagaa aatgtaattg aatggtgcaa agaaaaaatt agcctttcta
     4801 atgcggaaat cacaagaaaa ggaaaaaact ggtatataac gattgataat tgtattataa
     4861 cggtaaatgc gtacagttat accgttatca ctgctcacaa ggtaaaatga taaatttttc
     4921 tataaaggaa ttaaacgcat ttgcagttat tgggcaggaa gtagaactga ctaattatca
     4981 aaaaaagaat attcagatta gtacacagtt ttggagaaag tttaacagta gtttgaaaaa
     5041 agcgtatctt tcacaatcag gaaattgggt aaaatatgct tttatggaaa gaagaaatgg
     5101 aaaactttat tatttctgtt ctattcccca aagaaccatt accccagagg gctttctgta
     5161 taaagaaata ccgtcttata aatatttggt tgtggagcat attggtgcta tggataaaat
     5221 atatgaaact tatggcaaga tttatgaaga aattattcct agtacatcgt atattggctc
     5281 tttgtcaaat gatgttggta aagagaaatc tta
//
LOCUS       DAJSKS010000364         4803 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.guided.171, whole genome shotgun sequence.
ACCESSION   DAJSKS010000364 DAJSKS010000000
VERSION     DAJSKS010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.guided.171"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            92..787
                     /gene="vanR"
                     /locus_tag="ON396_003030"
     CDS             92..787
                     /gene="vanR"
                     /locus_tag="ON396_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCR3115037.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            765..1919
                     /locus_tag="ON396_003031"
     CDS             765..1919
                     /locus_tag="ON396_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCR3115038.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2134..3102
                     /locus_tag="ON396_003032"
     CDS             2134..3102
                     /locus_tag="ON396_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HCR3115039.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3095..4126
                     /gene="vanA"
                     /locus_tag="ON396_003033"
     CDS             3095..4126
                     /gene="vanA"
                     /locus_tag="ON396_003033"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HCR3115040.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4132..4740
                     /locus_tag="ON396_003034"
     CDS             4132..4740
                     /locus_tag="ON396_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HCR3115041.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1482 a          922 c         1119 g         1280 t
ORIGIN      
        1 catggccttt tatcttttac acaattatat ggactttatc ctataatctg ataaattatt
       61 aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta ttgtggatga
      121 tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt atacggtttt
      181 caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga ttgaccttgc
      241 catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa aaataaggga
      301 caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag ataaaattac
      361 agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac tggagttaat
      421 tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa aggagcagaa
      481 cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg agtgttatct
      541 gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa tcctctgtga
      601 aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg gcgacgaata
      661 tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg aaaaaatgaa
      721 cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt ataaaattga
      781 aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat atcgttgcaa
      841 ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga gggaaacttg
      901 gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg gacgcgatga
      961 aattatatca atattccata cggaacaata tagatatctt tatttatgtg gcgattgtca
     1021 ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac tttgacgaga
     1081 taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag ctttctgcgg
     1141 aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa aagcgagagc
     1201 aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg gcgcacgata
     1261 ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag gctccagaca
     1321 tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg tatcgactcg
     1381 aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg ataacgctaa
     1441 caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa ttttatcctc
     1501 agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg accgtgtccg
     1561 gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc gctgcataca
     1621 gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg gtgtcaatcg
     1681 aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt gaaaagttct
     1741 ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga ttggcgattg
     1801 caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat gataactata
     1861 cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg aggtcctaag
     1921 agatgtatat aattttttag gaaaatctca aggttatctt tactttttct taggaaatta
     1981 acaatttaat attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga
     2041 gccgttttcg ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt
     2101 tggtgccttt cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat
     2161 ggatgtgagc aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg
     2221 gcaacgataa ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa
     2281 tgtatcagtg tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga
     2341 gccggtgtga aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct
     2401 gctaagagaa tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat
     2461 tatactatga tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg
     2521 gaaaaacatg atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt
     2581 ggtgtggtgg gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga
     2641 tgtaaagtgt tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat
     2701 gagttgctgc aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac
     2761 tatattatca gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact
     2821 gggcgcggtc cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg
     2881 ggcggtgccg cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc
     2941 caaaaaccaa ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc
     3001 acaccgcata cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt
     3061 aaaaactgtt tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact
     3121 gtttgggggt tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc
     3181 taacattaat aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg
     3241 gaaaatgtgc gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact
     3301 ctcgccggat aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa
     3361 ccatgttgat gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca
     3421 aggtctgttt gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat
     3481 ttgtatggac aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc
     3541 cttttgggtt attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt
     3601 tgttaagccg gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga
     3661 attggactac gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc
     3721 tgtttcgggc tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagttgttgg
     3781 cgaggtggac caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc
     3841 ggaaaaaggc tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg
     3901 aggacggata caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc
     3961 ccgtgtggat atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct
     4021 gcccggtttc acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact
     4081 tcccgaactg attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata
     4141 ggatttactt ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact
     4201 tgggataatt tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca
     4261 tacgagttgg ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga
     4321 ttgcttctat gggacggtta ccgtcctaag cgtgctgtaa actgttttat gcaatgggct
     4381 gcacagccgg aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag
     4441 atgatttcaa aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat
     4501 cttacgcttt atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt
     4561 atggatgaac gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc
     4621 agacgtttgc gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg
     4681 cactatgtat taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa
     4741 acttttaacc gttgcacgga caaacggttc tgttgcaaag ttttaaataa agaataaaat
     4801 ccc
//
LOCUS       DAJSKS010000365         4034 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.guided.188, whole genome shotgun sequence.
ACCESSION   DAJSKS010000365 DAJSKS010000000
VERSION     DAJSKS010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.guided.188"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            complement(1..998)
                     /locus_tag="ON396_003035"
                     /pseudo
     CDS             complement(1..998)
                     /locus_tag="ON396_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
     gene            1489..2427
                     /locus_tag="ON396_003036"
     CDS             1489..2427
                     /locus_tag="ON396_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR3115042.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            2557..3204
                     /locus_tag="ON396_003037"
     CDS             2557..3204
                     /locus_tag="ON396_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR3115043.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(3897..4034)
                     /locus_tag="ON396_003038"
                     /pseudo
     CDS             complement(3897..4034)
                     /locus_tag="ON396_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
BASE COUNT         1350 a          584 c          674 g         1426 t
ORIGIN      
        1 aatacaatat tgttgccaca cttaggacat tttcctacaa tacttttttc tgcttctttt
       61 tctttctgtt cctggtaatc agaaaaattt agtttttcta tatcgttagg tacagcttcc
      121 agtaaatgaa caatgaattt tttgatattc gtaataaagt tctcttgatt gccttctctt
      181 ttaccgattt tttttaaata cgtttcccat ttagccgtca tttcagcact cgttaaaagg
      241 tgctgacttt caactgcctg gcacaataat tttccttttt cagttacaac aagcttattc
      301 ttaatcactt ggatatattc tttttgtttt aacgcttcaa taatacttgc tcttgtcgct
      361 tctgttccaa tcccctcaac ttcttgtaaa atcttgattg cttcttcatc atcaaccgtt
      421 ttattcgccg ttttcatagc agttaataat gtaccctctg taaaagcttt tggcggttgc
      481 gtttcttttt caacactctt aacaccaact tcggcacgtt cgccaatgat aacgagtggt
      541 aacgtttgaa cgtcctcttt ttcttctttg gtttgttgtt tgaaaagaat tttccaccct
      601 tcttgctttg gtgtctttcc gatcgattga aacagcaaat cagctacttt cgtttgtatc
      661 ttggtttctt catacaagta atcaggtaaa aacatggcaa cggtcgtttt aacgactaaa
      721 gcataaattt ttcgttgtaa atcgtccatt ttcgctaatg cagattcggt aggtacttgt
      781 tttgttggga taatggcgtg gtgttcctgt accttactac cgtccacata acgctttcta
      841 ggctctgttt gaaccatttc aagatcaagt cctaaaaaac cgctatattt gccaaaattc
      901 gcttttaaat aagcaaattc gttctctgta ataaatggtg tatctgttct tggataactc
      961 aataattttg cttcatacag tccttgcata gcttttaaac gtaaaatttt gcgttctaag
     1021 acgttttaaa tatatctgcg tatattttta tgtgttttat cttttcgtcg gcaaatgcgt
     1081 taaaatttgc gaaataaaat agctcaaggg cgaacgcagt gagtgcattt acccttgatc
     1141 tatttttttg agtaaattag cttttgaaac cgacggaaag gaaacacaaa acttttcaga
     1201 ggcaaggggt ttttaagggg cgcttttagc cccttgaatt tatttgcttt aagatatatt
     1261 tagttttaaa atagttttaa cgaaaaaaag acataaaaaa agacgagcaa cgctaccaac
     1321 gttctcgccc ggaataacaa accacacttg ttattcattc gatgattagt atatcaattt
     1381 ttgattttga atacaaaaca aaatggacga atggatacag gcgaaattta ttttgcgatt
     1441 ccactcgtcc ttttttcgtt ccaaaaatta ttttaggagc gataaaaaat gaagaaaatt
     1501 gatgaaaaat tagctgcaga aatctcacta aaaaaaccgg ccgtctctaa tagccggtta
     1561 agtaatagcc ggttaagtgg tcaaactttg ggaaaatctc aacccgcatt aagttttgat
     1621 gccatgacaa tcgttggaaa tttgaacaaa actaatgcta aaaagctatc tgactttatg
     1681 agtgtagagc cacaaatacg actttgggat atacttcaaa caaagtttaa agctaaggca
     1741 cttcaagaaa aagtttatat cgaatatgac aaagtaaaag cagatacttg ggatagacgt
     1801 aatatgcgtg ttgaatttaa tcccaataaa ctcacacatg aagaaatgat ttggttaaaa
     1861 caaaatatta tcgactacat ggaagatgac ggttttacaa gattagactt agcttttgat
     1921 tttgaagatg atttgagcga ttactatgca atgactgata aagcagttaa gaaaactgtt
     1981 ttttatggtc gtaatggcaa gccagaaaca aaatattttg gtgtccgtga tagtgataga
     2041 tttattagaa tttataataa aaaacaagaa cgtaaagata acgcagatgt tgaagttgtg
     2101 tctgaacatt tatggcgtgt agaagttgaa ttaaaaagag atatggttga ttactggaat
     2161 gattgtttta atgatttaca catcttgaaa cctgcgtggg ctactttaga aaaaattaat
     2221 gagcaagcta tggtttatac tttgttgcat gaagaaagta tgtggggaaa gctaagtaag
     2281 aatactaaga ctaaatttaa aaaattgatt agagaaatat ctccaattga tttaacggaa
     2341 ttaatgaaat cgactttaaa agcgaacgaa aaacaattgc aaaagcagat tgatttttgg
     2401 caacgtgaat ttaggttttg gaagtaaaat aagttttatt tgataaaaat tgctaattca
     2461 gtataattaa tatttacgag gtgacataac gtatgaaaaa atcagaggat tattcctcct
     2521 aaatataaaa atttaaaatt taggaggaag ttatatatga cttttaatat tattgaatta
     2581 gaaaattggg atagaaaaga atattttgaa cactatttta atcagcaaac tacttatagc
     2641 attactaaag aaattgatat tactttgttt aaagatatga taaaaaagaa aggatatgaa
     2701 atttatccct ctttaattta tgcaattatg gaagttgtaa ataaaaataa agtgtttaga
     2761 acaggaatta atagtgagaa taaattaggt tattgggata agttaaatcc tttgtataca
     2821 gtttttaata agcaaactga aaaatttact aacatttgga ctgaatctga taaaaacttc
     2881 atttcttttt ataataatta taaaaatgac ttgcttgaat ataaagataa agaagaaatg
     2941 tttcctaaaa aaccgatacc tgaaaacacc ataccgattt caatgattcc ttggattgat
     3001 tttagttcat ttaatttaaa tattggtaac aatagcagct ttttattgcc tattattacg
     3061 ataggtaaat tttatagtga gaataataaa atttatatac cagttgctct gcaacttcat
     3121 cattctgtat gtgatggtta ccatgcttca ctatttatga atgaatttca agatataatt
     3181 catagggtag atgattggat ttagttttta gattttgaaa gtgaatttaa ttttatacac
     3241 gtaagtgatc ataaaattta tgaacgtata acaaccacat tttttggttg cttgtggttt
     3301 tgattttgaa tttggttttg aacttatgga ctgatttatt cagtccattt tttgtgcttg
     3361 cacaaaaact agcctcgcag agcacacgca ttaatgactt atgaaacgta gtaaataagt
     3421 ctagtgtgtt atactttact tggaagatgc accgaataaa aaatattgaa gaacaactag
     3481 caaaagattt taaagagtta ttttatttta agtctttata acatgagtga agcgaatttt
     3541 taaatttcga tagaaatttt tacatcaaaa agccccctgt caaaattgac gaagggggtt
     3601 ttttggcgca cgcttttcgt tagaaatata caagattgaa aatcgtgtat aagtgcgccc
     3661 tttgttttga acttagcacg ttacatcaat tttttaaaat gatgtataag tgcgcccttt
     3721 taaattttga gtgattatat tttttgagtt agaaaaaggg attgggaaaa tttcccaaaa
     3781 taatttaaaa aataagcaaa aattttcgat agagaatgtg ctattttttg tcaaaggtgt
     3841 ataccttgac tgtgcttgct gttacattaa gtttattttt aagttattaa aaaagaaata
     3901 gcttttaaag tttggctcgc tgtcgcttta taaagctgat tgacttttga ttgcaaacta
     3961 cttaaagaaa acaaactcgg actattcgtt ttcttctctt tggtttgaac atcagcaatt
     4021 atcccctctt gatt
//
LOCUS       DAJSKS010000366         3278 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.guided.203, whole genome shotgun sequence.
ACCESSION   DAJSKS010000366 DAJSKS010000000
VERSION     DAJSKS010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.guided.203"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..188
                     /locus_tag="ON396_003039"
     CDS             <1..188
                     /locus_tag="ON396_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR3115044.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGEEIKISNE"
     gene            203..1072
                     /locus_tag="ON396_003040"
     CDS             203..1072
                     /locus_tag="ON396_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCR3115045.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="ON396_003041"
     CDS             1053..1787
                     /locus_tag="ON396_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR3115046.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="ON396_003042"
     CDS             1820..2728
                     /locus_tag="ON396_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HCR3115047.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..3205
                     /locus_tag="ON396_003043"
                     /pseudo
     CDS             2725..3205
                     /locus_tag="ON396_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1146 a          465 c          718 g          949 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagaattgga tgataaaaat agaaacaacc ttgttgtgcc
      421 tcccggagcg tggggtgatt ggattaatgg cggcggatgg ttatttataa atgggtatca
      481 tgttgacttg attttacgtg atataaaacg ggtagaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgcaatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggtag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgatatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc ttttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     3001 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     3061 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     3121 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     3181 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtg
     3241 gttctgttgc aaagttttaa ataaagaata aaatccct
//
LOCUS       DAJSKS010000367         1533 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.guided.255, whole genome shotgun sequence.
ACCESSION   DAJSKS010000367 DAJSKS010000000
VERSION     DAJSKS010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.guided.255"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            228..1103
                     /locus_tag="ON396_003044"
                     /note="aph(2'')-Ia"
     CDS             228..1103
                     /locus_tag="ON396_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HCR3115048.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEIYKRTYKD"
     gene            complement(1233..>1533)
                     /locus_tag="ON396_003045"
     CDS             complement(1233..>1533)
                     /locus_tag="ON396_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR3115049.1"
                     /translation="LFDSRFKTLQQNQPKYSKACASLDDGFEDAFQYTVQGNSHNRLK
                     STNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          610 a          144 c          260 g          519 t
ORIGIN      
        1 gtacaagata tattaaattg atttttgaat ttttgaaaaa agaaagaaat gctaatgcag
       61 ttattttaga ccctcataaa aataatccaa gagcaataag ggcataccaa aaatctggtt
      121 ttagaattat tgaagatttg ccagaacatg aattacacga gggcaaaaaa gaagattgtt
      181 atttaatgga atatagatat gatgataatg ccacaaatgt taaggcaatg aaatatttaa
      241 ttgagcatta ctttgataat ttcaaagtag atagtattga aataatcggt agtggttatg
      301 atagtgtggc atatttagtt aataatgaat acatttttaa aacaaaattt agtactaata
      361 agaaaaaagg ttatgcaaaa gaaaaagcaa tatataattt tttaaataca aatttagaaa
      421 ctaatgtaaa aattcctaat attgaatatt cgtatattag tgatgaatta tctatactag
      481 gttataaaga aattaaagga acttttttaa caccagaaat ttattctact atgtcagaag
      541 aagaacaaaa tttgttaaaa cgagatattg ccagtttttt aagacaaatg cacggtttag
      601 attatacaga tattagtgaa tgtactattg ataataaaca aaatgtatta gaagagtata
      661 tattgttgcg tgaaactatt tataatgatt taactgatat agaaaaagat tatatagaaa
      721 gttttatgga aagactaaat gcaacaacag tttttgaggg taaaaagtgt ttatgccata
      781 atgattttag ttgtaatcat ctattgttag atggcaataa tagattaact ggaataattg
      841 attttggaga ttctggaatt atagatgaat attgtgattt tatatactta cttgaagata
      901 gtgaagaaga aataggaaca aattttggag aagatatatt aagaatgtat ggaaatatag
      961 atattgagaa agcaaaagaa tatcaagata tagttgaaga atattatcct attgaaacta
     1021 ttgtttatgg aattaaaaat attaaacagg aatttatcga aaatggtaga aaagaaattt
     1081 ataaaaggac ttataaagat tgattatata atatatgaaa agctattata aaagacatta
     1141 gtattaaata gtttaaaaaa atgaaaaata ataaaggaag tgagtcaatt ccagactcct
     1201 gtgtaaaatg ctatacaatg tttttaccat ttctacttat caaaattgat gtattttctt
     1261 gaagaataaa tccattcatc atgtaggtcc ataagaacgg ctccaattaa gcgattggct
     1321 gatgtttgat tggggaagat gcgaataatc ttttctcttc tgcgtacttc ttgattcagt
     1381 cgttcaatta gattggtact ctttagtcga ttgtgggaat ttccttgtac ggtatattga
     1441 aaggcgtctt cgaatccatc atccaatgat gcgcaagctt ttgaatattt tggttggttc
     1501 tgttgcaaag ttttaaatct actatcaaat aag
//
LOCUS       DAJSKS010000368         1149 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.guided.282, whole genome shotgun sequence.
ACCESSION   DAJSKS010000368 DAJSKS010000000
VERSION     DAJSKS010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.guided.282"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            12..77
                     /locus_tag="ON396_003046"
     CDS             12..77
                     /locus_tag="ON396_003046"
                     /inference="COORDINATES: protein motif:HMM:NF018061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HCR3115050.1"
                     /translation="MFYTIIWTLSKQTKNSDYADK"
     gene            202..939
                     /gene="erm(B)"
                     /locus_tag="ON396_003047"
     CDS             202..939
                     /gene="erm(B)"
                     /locus_tag="ON396_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCR3115051.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          421 a          200 c          185 g          343 t
ORIGIN      
        1 catggccttt tatcttttac acaattatat ggactttatc gaaacagact aaaaacagtg
       61 attacgcaga taaataaata cgttagatta attcctacca gtgactaatc ttatgacttt
      121 ttaaacagat aactaaaatt acaaacaaat cgtttaactt ctgtatttgt ttatagatgt
      181 aatcacttca ggagagatta catgaacaaa aatataaaat attctcaaaa ctttttaacg
      241 agtgaaaaag tactcaacca aataataaaa caattgaatt taaaagaaac cgataccgtt
      301 tacgaaattg gaacaggtaa agggcattta acgacgaaac tggctaaaat aagtaaacag
      361 gtaacgtcta ttgaattaga cagtcatcta ttcaacttat cgtcagaaaa attaaaactg
      421 aatactcgtg tcactttaat tcaccaagat attctacagt ttcaattccc taacaaacag
      481 aggtataaaa ttgttgggaa tattccttac catttaagca cacaaattat taaaaaagtg
      541 gtttttgaaa gccgtgcgtc tgacatctat ctgattgttg aagaaggatt ctacaagcgt
      601 accttggata ttcaccgaac actagggttg ctcttgcaca ctcaagtctc gattcagcaa
      661 ttgcttaagc tgccagcgga atgctttcat cctaaaccaa aagtaaacag tgtcttaata
      721 aaacttaccc gccataccac agatgttcca gataaatatt ggaagctata tacgtacttt
      781 gtttcaaaat gggtcaatcg agaatatcgt caactgttta ctaaaaatca gtttcatcaa
      841 gcaatgaaac acgccaaagt aaacaattta agtaccatta cttatgagca agtattgtct
      901 atttttaata gttatctatt atttaacggg aggaaataat tctatgagtc gcttttttaa
      961 atttggaaag ttacacgtta ctaaagggaa tggagataaa ttattagata tactactgac
     1021 agcttccaag aagctaaaga ggtccctagc gcctacgggg aatttgtatc gataaggggt
     1081 acaaattccc actaagcgct cgggacccct tgtaggaaaa tgtcctaagt gtggcaacaa
     1141 tattgtatt
//
LOCUS       DAJSKS010000369          584 bp    DNA     linear   BCT 02-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium strain AUSMDU00051363
            SAMN29549366-rid15779893.guided.337, whole genome shotgun sequence.
ACCESSION   DAJSKS010000369 DAJSKS010000000
VERSION     DAJSKS010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29549366
            Sequence Read Archive: SRR20032845
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001474306.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001474306.1
            Genome Coverage       :: 134x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/03/2022 00:50:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,048
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,910
            CDSs (with protein)               :: 2,910
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 76
            CDSs (without protein)            :: 76
            Pseudo Genes (ambiguous residues) :: 0 of 76
            Pseudo Genes (frameshifted)       :: 39 of 76
            Pseudo Genes (incomplete)         :: 32 of 76
            Pseudo Genes (internal stop)      :: 19 of 76
            Pseudo Genes (multiple problems)  :: 13 of 76
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29549366-rid15779893.guided.337"
                     /strain="AUSMDU00051363"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2014"
     gene            <1..>584
                     /locus_tag="ON396_003048"
     CDS             <1..>584
                     /locus_tag="ON396_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR3115052.1"
                     /translation="MYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIF
                     EFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRY
                     DDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGY
                     AKEKAIYNFLNTNLETNVKIPNIEYSYISDELSIL"
BASE COUNT          245 a           50 c           97 g          192 t
ORIGIN      
        1 atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat agtctatggt
       61 atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac aagatatatt
      121 aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat tttagaccct
      181 cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag aattattgaa
      241 gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt aatggaatat
      301 agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga gcattacttt
      361 gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag tgtggcatat
      421 ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa aaaaggttat
      481 gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa tgtaaaaatt
      541 cctaatattg aatattcgta tattagtgat gaattatcta tact
//
